LOCUS       DASSJH010000001         6074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1514666, whole genome shotgun sequence.
ACCESSION   DASSJH010000001 DASSJH010000000
VERSION     DASSJH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514666"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VFJ67_00005"
     CDS             complement(<1..912)
                     /locus_tag="VFJ67_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HET7288790.1"
                     /translation="MELKKLRTDAVAIFEAGLSAVDPENAVNAHLSLGDGALVVNGRS
                     YPLSEFENVYVIGAGKAAAPMARAVEDILGGRVTAGIINVKYGHTVGLRKVKINEAAH
                     PVPDEAGQSGASEIVKLLGGTGERDLVVFLISGGGSALLPLAAEGLTLEDKQILTAKL
                     LECGASIHEINALRKHVSRVKGGRLARLACPSTLISLILSDVIGDDLDSIASGPTVPD
                     RSTFGDCERIIEKYGLRGKLPERVVEYIDRGARGGEEETPKPGDPAFARTQNVIVGSN
                     TSAASAAKDKASALGYNSIVLSTYIDGE"
     gene            1062..1700
                     /locus_tag="VFJ67_00010"
     CDS             1062..1700
                     /locus_tag="VFJ67_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288791.1"
                     /translation="MRNALRTASIPLLLLLFVCIYIARGADDPGPVSCPESCAGSRKN
                     CELSCSQIVGGGVESGKKRECFGACADEEVSCNERCLNPTPRPTLKPGAYSDESCSGA
                     CEFMSRDCKEACTKHVGGGAAGIERAKCENACDNELGKCTSHCSSPTPAPVVDPGVYE
                     NNPCSGECSSELEKCEGNCAMFSGESENGGKRGECMTGCKGAEYNCLEACPR"
     gene            complement(1726..2286)
                     /locus_tag="VFJ67_00015"
     CDS             complement(1726..2286)
                     /locus_tag="VFJ67_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HET7288792.1"
                     /translation="MDRERKNILITGLPGIGKTTLIKQLVYELSRYGPRGFYTEEIRP
                     KEERIGFQLVSLDGKRSMLAHVLIDSPYQIGKYRVDVSAFDSFLDSLRLGMPEGGIVI
                     IDEIGKMECLSMKFVRMIQELLDSGNIVIATIAHTDGGIKGRIKERDDVMLFKMNLEN
                     RDVLKDEITGALRDMPVFERLRAESG"
     gene            complement(2371..3513)
                     /locus_tag="VFJ67_00020"
     CDS             complement(2371..3513)
                     /locus_tag="VFJ67_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET7288793.1"
                     /translation="MEYREIYLDNNATTKPLPEVREEVYKILGDEFGNPSSAHSVGER
                     ARKHLSNAREKTAALIGSRPSRLIFTSSGTESNNMAFYSRTRNKGKKYRVITTTVEHS
                     SIRKMCSYLCINGVEIKLIKVDSKGLIDLDELRKAINGDTTLVSVQWVNNETGVIQPV
                     IEAGEMSRENGVAFHTDAAQAVGKIEMSVEELPFDFLTFTAHKFHGPQGAGAIYTKDK
                     FLLAPILFGGFQEEGFRPGTENLPGIVGLGKAAELRKKGLASLTAHMKELRDRFEAMV
                     LDFVPGTRVNGDVRRRICNTTNIMFGGIDGRKLVRELDAAGVMCSQSSACTNFEVSPS
                     YVLCSMGLSESEAYSSIRFSFSAENSFEEADRAVGLIRDICDRLRT"
     gene            complement(3627..4025)
                     /locus_tag="VFJ67_00025"
     CDS             complement(3627..4025)
                     /locus_tag="VFJ67_00025"
                     /inference="COORDINATES: protein motif:HMM:NF042459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6573 family protein"
                     /protein_id="HET7288794.1"
                     /translation="MTADARTRKQALEEGRQRDISETAREAGLLVPVFITSTVWDNWI
                     ASGVSGIKTEEVEKRKLRSVLDKLLYFIRVHRQTNKSNLIYFPVPLTREGKEENVQLM
                     SHLGPLERADQRPCITIMTPEEYDLESGVQ"
     gene            complement(4043..4867)
                     /locus_tag="VFJ67_00030"
     CDS             complement(4043..4867)
                     /locus_tag="VFJ67_00030"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7288795.1"
                     /translation="MKNIIKAALLVLAVLISADFARTVFSEEAALRFPKGEWIDLTHD
                     FSSDTVYWPTGKGFKLEKVSEGTTVEGYYYLANKYSAEEHGGTHIDAPVHFAKGRQTL
                     DQIPLENLIGSAVVIDVSKKAASDRDYLISVADFTEWESVNGRIPEGAIVLLNTGYAK
                     YWPDRVKYMGTDKKGQEAVKDLHFPGLDPAAAKWLTEKRSISAIGLDTPSIDYGQSAL
                     YESHRILFDKNIPAFENVANLDMLPAKGALVVALPMKIKGGSGGPLRIVALIPATD"
     gene            complement(4890..5675)
                     /gene="budA"
                     /locus_tag="VFJ67_00035"
     CDS             complement(4890..5675)
                     /gene="budA"
                     /locus_tag="VFJ67_00035"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET7288796.1"
                     /translation="MTVIRYSVLLTAIFMTSALARPAYAGDVIFQTSTLDALMEGVYD
                     GNLTYGELKKHGDFGLGTFNDLDGEMIGLDGVFYQVKSDGKVSVVPDDARTPFAAVTF
                     FEPDESVELTEKLGCRELENYIESLLLSKNILYALKIEGRFDYVKTRSVPRQEKPFPP
                     LTEAVKRETYFELKNVKGTMAGFWLPGYVKDINATGFHLHFITDDRKAGGHVLDCTVR
                     DVKIGIDYSRGLDISLPDTSSFYNADLTGASGSDVSSVEKGKK"
     gene            complement(5689..>6074)
                     /locus_tag="VFJ67_00040"
     CDS             complement(5689..>6074)
                     /locus_tag="VFJ67_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7288797.1"
                     /translation="PQVSVTKRIFIISSRPNVRYPNAPVMEPEAVINPEIISRSEETV
                     KDWEGCLSIPGIRGLVPRHMWVEVSYVSREGNVVERRFSDFIARIFQHEYDHLNGVLF
                     LDRIISSTDLITEKEYVKLLRRETE"
BASE COUNT         1107 a         1828 c         1635 g         1504 t
ORIGIN      
        1 ctcgccgtcg atatatgtcg agagtacgat tgaattatat ccgagagccg aggctttgtc
       61 tttcgcggct gaagcggctg aggtattgct ccccactatg acgttctgag tgcgcgcgaa
      121 ggcggggtct cccggtttcg gcgtctcctc ctcaccgccg cgtgcgcccc tgtctatgta
      181 ttcgacgact ctctccggca gcttcccgcg gaggccgtat ttctcgatga tcctctcgca
      241 gtccccgaac gtgctcctgt cgggcactgt cggtcccgat gcaatcgaat cgaggtcgtc
      301 cccgatgacg tccgaaagga taagggatat cagggtcgaa gggcaggcga gtcttgcgag
      361 cctgcccccc tttacgcggg agacgtgctt ccggagcgcg tttatctcat gtatggacgc
      421 gccgcattcg aggagcttcg ccgtgagtat ctgcttgtct tcgagcgtta gcccttccgc
      481 cgccaggggc aggagcgccg accctccgcc ggagatgagg aatacgacta ggtcccgttc
      541 tccggtgccg cccagcagtt tcacgatctc gctcgctccc gactgcccgg cctcgtcggg
      601 caccgggtga gcggcttcgt ttatctttac cttcctgagc ccgacggtgt gcccgtattt
      661 cacgtttatt atccccgccg taacgcgccc gcctagaatg tcctcgactg cccgggccat
      721 cggcgctgcc gctttcccgg cccctattac atatacgttc tcgaactcgg atagcgggta
      781 gctcctcccg tttacgacga gcgccccgtc cccgagagag aggtgcgcat tgaccgcgtt
      841 ttcggggtca accgcgctca ggcccgcctc gaatatggcg accgcgtcgg tcctcagctt
      901 tttcaattcc attggttgac agtataacga gcgcctccgt atgtaacaac aaacctgcgt
      961 cttgctccgg cttgacgatt tttgaggccg ccctcaccgg gattttaaaa tctccgaaat
     1021 cacgggtaga ttcttcggta tgccggttgc cggataaaac catgcgaaac gctctcagaa
     1081 ccgcttcgat accgctcctt cttttgctct ttgtttgtat atacatcgcc cgcggcgctg
     1141 acgatcccgg cccggtctcg tgtcccgaga gctgcgccgg ctccaggaaa aactgcgagc
     1201 tctcctgttc tcaaatcgta ggcggcgggg tcgagagcgg taagaagagg gagtgcttcg
     1261 gagcctgcgc cgacgaggag gtttcctgca acgagaggtg cctcaacccc acgccgcgtc
     1321 ccacgctcaa gcccggggct tacagcgacg agtcgtgctc gggagcctgc gagttcatga
     1381 gcagggactg taaagaggcg tgcacgaagc atgtgggcgg cggcgcggcg ggcatagaac
     1441 gggctaaatg cgaaaacgcg tgcgataacg agctcggtaa atgcacgagc cactgctcga
     1501 gcccgacccc cgctccggtc gtagaccccg gcgtctacga gaacaacccc tgttctggtg
     1561 agtgctcttc ggagctcgag aagtgcgagg ggaactgcgc tatgttttca ggtgagagtg
     1621 agaacggcgg gaagcgcggc gaatgcatga ccgggtgtaa aggtgcggaa tataattgcc
     1681 ttgaagcctg cccgcggtaa gggttacacg tcaggcgcag caggtctatc cgctttccgc
     1741 ccgcagcctc tcgaacaccg gcatatcccg gagcgctccg gttatctcgt cttttaaaac
     1801 gtccctgttc tcgaggttca tcttgaagag cataacgtca tcccgctcct tgatcctgcc
     1861 ctttatgccg ccgtccgtat gggctatcgt cgcgatgacg atatttcccg agtccagaag
     1921 ctcctgtatc atacgcacga atttcatcga gagacattcc atcttgccta tctcgtctat
     1981 gatcactatg ccgccttcgg gcatgccgag tcggagcgaa tcgaggaacg agtcgaacgc
     2041 cgagacgtcc accctgtatt tccctatctg gtaggggctg tcgataagca cgtgggcgag
     2101 catcgacctc ttcccgtcga ggctcacgag ctgaaaccct atcctctcct ccttggggcg
     2161 gatctcctcg gtatagaacc cgcgcgggcc gtatctcgag agctcgtaaa cgagctgctt
     2221 tatgagtgtg gttttcccta ttccgggtag ccccgtaata aggatgttct ttctttccct
     2281 gtccatttcg tcttgttccg tgaatttata gaaaggaggt tattcaccgc tgccgcaggc
     2341 cccggttgag gtttggtgag cgggatgtat ttacgttctg agcctgtcgc agatgtccct
     2401 gatgagacct acggccctgt ccgcttcttc gaatgagttc tccgcggaaa aactgaaccg
     2461 gatgcttgag tacgcctcgg actcggacag tcccatcgag cacaatacgt acgagggcga
     2521 gacctcgaag ttggtgcatg cagagctctg ggagcacatg acgcccgcag cgtcgagctc
     2581 cctcacgagc ttccttccgt ctattcctcc gaacatgata ttcgtcgtgt tgcagatcct
     2641 ccgccttacg tctccgttca ctcttgtccc cggcacgaaa tcgagcacca tggcttcgaa
     2701 cctgtccctc aattccttca tgtgtgcggt gagactcgca aggcccttct ttctcagctc
     2761 cgcggccttg ccgagcccga ctatgcccgg caggttctcg gtcccgggcc tgaacccctc
     2821 ttcctggaac cctccgaaca gtatcggcgc gaggaggaat ttgtccttcg tgtaaatggc
     2881 cccggccccc tggggtccgt ggaatttgtg agccgtgaat gtaaggaaat cgaatgggag
     2941 ctcctctacg ctcatttcga tcttccccac cgcttgcgcg gcgtccgtat ggaacgccac
     3001 accgttctcc cggctcatct cccccgcttc aatcacgggt tgtatgacgc ccgtttcgtt
     3061 attgacccac tggacggaaa cgagggtggt atccccgttt atcgccttcc tcagctcgtc
     3121 gaggtctatc aggcccttcg agtccacctt tatgagcttg atctcgacgc cgttgatgca
     3181 gaggtagctg cacatcttcc tgatggagga gtgctctacg gtggtggtga tgacccggta
     3241 cttcttcccc ttgttcctcg tgcgggaata aaacgccatg ttgttcgact ccgtccccga
     3301 gctcgtgaat atgagcctcg agggtctcga gccgatgagc gccgctgtct tttctcgggc
     3361 gttcgagagg tgcttcctcg ccctttcacc tacagagtgg gcgctcgacg ggttgccgaa
     3421 ctcgtcgcca agtattttat agacctcttc cctcacttcc ggaagcggct tcgtagtcgc
     3481 gttgttgtcg agatagattt ccctgtattc cataacccgg cccggctatg agatattata
     3541 cattatttgg cttgtttatt cgtacgcggt cccggtcctg aattccggcc ccgccggctg
     3601 ccgcaggccg gtccgggaag ttgcgattat tgcacgccgc tttcgaggtc gtattcttcc
     3661 ggggtcatta tagtaatgca gggcctctgg tcggcccttt cgagcgggcc caggtgcgac
     3721 atgagctgta cgttttcctc tttcccctcc ctcgtgagcg gcaccgggaa gtagatcagg
     3781 ttggacttgt tggtctggcg gtggaccctt atgaagtaaa ggagcttgtc gaggacgctc
     3841 ctaagcttcc ttttctcgac ttcttccgtt tttatgcccg aaactcccga tgcgatccag
     3901 ttgtcccaca ctgtcgatgt gatgaacacc ggcacgagca gccccgcttc cctggcggtc
     3961 tcggagatgt ccctctgcct gccttcttcg agcgcctgtt ttctcgtccg ggcatctgcg
     4021 gtcatttcaa tattgtacca gcttaatcag ttgcaggtat gagcgcgacg atcctgagag
     4081 gtcctccgct accgcctttt atcttcatcg ggagcgcgac gacgagagcg ccctttgcgg
     4141 ggagcatgtc caggttcgcg acgttctcga acgccgggat gttcttatcg aagagtatcc
     4201 tgtggctttc gtagagtgct gactgcccgt aatctatgct cggggtgtcg aggcctatgg
     4261 cgctaatgct ccttttctcg gtcagccact ttgcggcggc agggtcgaga cccgggaaat
     4321 ggagatcctt taccgcttcc tgtcccttct tgtccgtccc catatatttc accctgtccg
     4381 gccagtactt cgcatatccc gtgttgagca agacgatagc gccctcggga atccttccgt
     4441 tgacactctc ccattccgta aagtccgcga cgcttatgag gtaatcccgg tcggaagcgg
     4501 cttttttcga aacgtcgatc acgacagccg agccgataag gttttcgaga ggtatctggt
     4561 cgagggtctg cctgcccttt gcgaaatgta cgggcgcgtc gatgtgtgtc ccaccgtgct
     4621 cctcggccga gtacttattc gccaagtagt agtagccctc caccgtggtg ccctcggata
     4681 ccttctcgag cttgaaccct ttgcccgtag gccagtaaac agtatcgctc gaaaaatcgt
     4741 gcgtgaggtc gatccattct cctttaggga atcggagggc ggcctcctcg gagaataccg
     4801 tccgcgcaaa atctgcggag atgagaactg cgagaacgag tagagctgcc ttgataatgt
     4861 tcttcatctt tcacccttgc tgataatgtt tatttctttc ccttctccac actgcttacg
     4921 tcgctcccgc tcgctccggt caggtccgcg ttataaaaac tgctcgtgtc gggaagggat
     4981 atatcgagcc ctctggagta atctatcccg atcttaacgt ctctgacagt gcagtcgagc
     5041 acatgccctc cggctttcct gtcgtccgtg atgaaatgca ggtgaaaccc cgtcgcattt
     5101 atgtccttca cgtacccggg gagccagaac cccgccatcg tccccttcac gttctttagc
     5161 tcgaagtagg tctcccgctt gaccgcttcc gtgagcggcg ggaacggttt ctcctgtctc
     5221 ggaacgctcc tcgttttaac gtaatcgaaa cgcccctcta tcttaagcgc gtagagaata
     5281 ttttttgaga ggaggagcga ttcgatgtaa ttttcgagct ctctgcatcc gagcttctcc
     5341 gtaagctcga cactctcgtc gggctcgaag aacgtcaccg ctgcgaaagg ggtcctagcg
     5401 tcgtcgggca cgaccgagac ctttccgtcc gatttcacct gataaaagac cccgtcgagc
     5461 ccaatcatct caccatctaa atcgttgaac gtgccgagac cgaagtcccc gtgctttttc
     5521 agctcgccgt acgtgaggtt cccgtcgtaa accccttcca taagggcgtc cagggtcgag
     5581 gtctgaaaga taacgtcgcc cgcgtatgca gggcgcgcga gggccgaggt catgaagatt
     5641 gcggtcagca gtacggaata tcgtattacg gtcatgtata tctccctgtc attcggtctc
     5701 tctccgcagc agtttcacgt attccttttc cgttatcagg tccgtactcg aaataatacg
     5761 atctaggaaa agcactccgt tcaagtggtc gtattcgtgc tggaatatcc tcgcgatgaa
     5821 gtccgagaac cgcctctcca ccacattccc ctccctcgag acataactga cctcgaccca
     5881 catgtgacgt ggcacgagcc cccttatccc cggtatgctg agacagccct cccagtcctt
     5941 gaccgtttcc tctgagcgcg agattatttc ggggtttatc acggcttcgg gctccattac
     6001 gggcgcgttc ggatacctga cgttcgggcg ggaggagatt atgaatattc gtttcgtcac
     6061 actcacctga ggag
//
LOCUS       DASSJH010000002         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_163, whole genome shotgun sequence.
ACCESSION   DASSJH010000002 DASSJH010000000
VERSION     DASSJH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..986
                     /locus_tag="VFJ67_00045"
     CDS             54..986
                     /locus_tag="VFJ67_00045"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET7288798.1"
                     /translation="MKCPSCGYNSFDHLEYCKKCGSPIGREAESQTSETGERDSKKSR
                     ARPEKESAGPVTGELFLDIPIRDTEPHTRPEARGTGAARRSRTSGPGTEPEAESYELF
                     PASKGSEEGSLAGVRPGVVHTGAQETQPGPEHGLDAPQPDPFAFIENDGGAYEPEPDR
                     PEERYGREADVYNLAGFIPRALALAIDIVVVSLIALLAYFAGLFVLYGFDFGSYGSGN
                     MQKPLYMTLFLLSSTYFVFLHGYGGMTVGKMALGIKLINRYGEGVGIWDAFIRWIGYY
                     ISGAFLCAGFLWSLVDSEGQTWHDKIAGTYVVKD"
     gene            994..2253
                     /gene="gshA"
                     /locus_tag="VFJ67_00050"
     CDS             994..2253
                     /gene="gshA"
                     /locus_tag="VFJ67_00050"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF020460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET7288799.1"
                     /translation="MRHIDELNIRISENRKKIESWIRGKTEKEFIPLYSSVDVRVSNH
                     KLAPVDTNIFPAGFNNLSQSFRDSASGLFRDYFLRKYPKVKSILIIPELHTRNPYYWE
                     NILVLKSILEKVDYEVEVGIVSDDFDRDHASFQSQSGAEVEAYKVMKEDNRIFTSKMS
                     PDLLLINNDFSEKCPKTLKDIVQPVEPPVEIGWHTRRKSVHFEYYNKLAGEVSKLLGI
                     DPWMITIDTVAVEGIDFDSPGDRERAAEVADSMLERLRKTYSEKGIPEEPYLFLKSNS
                     GTYGMAVISVSDGDEVRRLNAEGRKRMRVSKGGKPVRDIVIQEGIPTALKLESGASAE
                     PVFYLVEAEVAGGFLRVNKGKDDHENLNTKGMEFSPMPGEGEGAGGDGRLIFSPAHEL
                     VSCIASIAAGYEIEAILREGGCKEETP"
     gene            complement(2378..>3631)
                     /gene="clcA"
                     /locus_tag="VFJ67_00055"
     CDS             complement(2378..>3631)
                     /gene="clcA"
                     /locus_tag="VFJ67_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008988487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HET7288800.1"
                     /translation="WSLIVGALTGLAGAVFQLSIEKVQDSRELLLRNFPQSSAFQLAA
                     SVLFSAVLVYIAFLLVRRLAPEASGSGVHEIEGALDDVRPIRWKNVLPVKFLGGLCSL
                     GGGMVLGREGPTIQMGGNIGKMVADIFRADREDTHALIAAGAGGGLTAAFNAPLAGIL
                     FVFEEMRPEFKYNFLSVQSVIIASVASDIALRSIMGQSPDIPMKMLATPPLTSLWIFL
                     IFGILFGIFGVLFNFLLVKTLDFFHNLKGPLFTLTGLYVGAFVGLLAWLYPNLIGGGY
                     KVIPEAISGTIPVTLLLLLFFGRFGTTMISYGSGAPGGIFAPMLALGTLFGMWYGSIA
                     HSYVPDLIVRPELFAVAGMAALFCATVRAPLTGIALTIEMTGNYDMILPLILTCLTAT
                     LVAEGLGGKPVYTVLLRRTLHLVKK"
BASE COUNT          919 a          909 c         1044 g          759 t
ORIGIN      
        1 attttttttg taagtggttt tgtcctctat aatttgttct gctgattaaa ccgatgaaat
       61 gcccgtcgtg cggatacaac agctttgacc atctcgaata ctgcaaaaaa tgcgggtctc
      121 cgataggcag ggaggctgaa tctcaaacgt ccgagaccgg ggagagggac agtaaaaaat
      181 cccgagcgcg cccggagaaa gagtcagccg ggcctgtaac gggcgagctg tttctcgaca
      241 taccaataag ggatacggag ccgcatacac ggcccgaagc ccggggcaca ggagctgcac
      301 gtcgcagtcg gacatccggc cccggcacgg agcctgaggc tgaatcttat gagcttttcc
      361 ctgcctctaa aggtagtgag gaagggagcc tcgcgggtgt aaggccggga gtcgtgcata
      421 ccggcgctca ggaaactcag ccgggcccgg aacacggact cgatgctccc cagccggacc
      481 ctttcgcttt tatcgaaaac gacggtggag cttacgaacc ggaacctgac aggccggaag
      541 aacggtacgg gcgggaggcc gacgtataca acctcgccgg ttttataccg cgcgccttag
      601 cgctggcaat agatatcgtc gtcgtatcat tgatagccct gctcgcgtat tttgcgggtc
      661 ttttcgtttt atacgggttc gatttcggat cgtacggttc cgggaatatg cagaagcctc
      721 tatacatgac tctctttctg ctctcctcca catacttcgt gtttcttcac gggtacggag
      781 gtatgactgt gggaaagatg gcattgggta taaaattgat aaacagatac ggcgaaggag
      841 taggcatttg ggacgcgttt ataagatgga tcggttatta catatccggg gcgtttttgt
      901 gcgccgggtt tttatggtcg ctggtggatt ccgaaggtca gacgtggcat gataaaatcg
      961 ccggaacata cgtcgtaaag gattgagacg ccgatgaggc atatcgacga actgaacatc
     1021 agaatttccg aaaacaggaa aaaaatcgaa tcctggatca ggggaaagac cgaaaaggag
     1081 ttcatacccc tttactcctc tgtggatgta agggtatcga atcataagct ggccccggtc
     1141 gataccaata ttttccccgc tgggttcaac aacctgtcgc aatccttcag ggacagtgcg
     1201 tctggtctat tcagggatta ttttctccgt aagtacccaa aagtgaaaag catccttatc
     1261 atacccgagc tccatacgag aaacccgtat tactgggaga acattctcgt gctcaaatcc
     1321 atactcgaga aagtcgatta cgaggtggaa gtcggtatcg tcagcgatga ttttgaccgc
     1381 gaccacgcaa gcttccagtc gcagagcggg gccgaggtcg aggcgtataa ggttatgaag
     1441 gaagataaca ggatattcac gtcgaagatg tctcccgacc tcctgctgat aaataacgac
     1501 ttctccgaga agtgtcccaa gaccctgaaa gacatcgtac agccggtcga gccgcctgtg
     1561 gagataggct ggcacacgag gagaaagagc gtccatttcg agtactataa caagctcgcg
     1621 ggagaggtgt ccaagctcct cggcatcgac ccgtggatga ttacgataga tacggtcgcg
     1681 gtggaaggca tcgacttcga tagccccggc gacagggaaa gggcggccga ggtcgcggac
     1741 tcgatgctcg agagattaag gaaaacatat agcgaaaaag ggataccgga agagccgtat
     1801 ttgttcctaa agagcaattc aggtacttac ggcatggccg taataagcgt ctcggacggc
     1861 gacgaggtac gcaggctcaa tgccgagggc aggaagcgga tgagagtcag taagggcggc
     1921 aaacccgtga gggacatagt catacaggaa gggataccca ccgcacttaa actggaatca
     1981 ggcgcgtcgg cagagcctgt attctatctc gtcgaagctg aggtcgccgg agggttcctg
     2041 cgagtgaata aggggaagga cgaccacgag aacctgaaca caaagggcat ggagttttct
     2101 ccgatgcccg gtgaaggcga gggagccggg ggcgacggca gattgatatt ttctcccgct
     2161 cacgagctcg tatcgtgtat agcgagcata gccgcggggt atgagatcga ggccatattg
     2221 agagagggcg ggtgtaagga agagaccccc tgactcaggt ctggttcatt tccccggctc
     2281 cgaacgtacc gttcaaatca cttcaagaaa ttctgtttat ccgatcccga taaagcagag
     2341 tcttaaatta agttgaagga atgcttgcaa tccgcggtca ttttttgacc agatggagtg
     2401 ttcttctaag cagcaccgtg taaacgggct ttcctcccag accttccgca acgagcgtgg
     2461 ccgtgaggca tgtgagtatg agcgggagta tcatgtcgta attacccgtc atctctatcg
     2521 tgagcgcgat gcccgtaagg ggcgccctta ccgtggcgca aaagagggcc gccatgccag
     2581 ccacggcgaa aagctccggg cgcacgatga ggtcgggcac gtaactgtgc gcaatgctgc
     2641 cgtaccacat gccgaagagc gtgccgaggg cgagcatcgg cgcgaaaatg ccgcctggag
     2701 cccctgagcc gtagcttatc atcgtggttc cgaaacgtcc gaagaaaagg agaagcagca
     2761 gcgtaaccgg gatagtaccc gaaatcgctt ccggtataac cttgtagcct cctccgatga
     2821 ggttcggata gagccatgcg aggagaccga cgaatgctcc cacgtaaaga cccgtgagag
     2881 tgaacaaggg gcctttgaga ttatgaaaga aatcgagtgt ctttacgaga aggaaattga
     2941 agagcacacc gaatatcccg aacaaaatac cgaatatgag gaatatccac agagacgtga
     3001 gcggcggggt ggcgagcatt ttcataggta tatccgggct ctggcccatt attgagcgga
     3061 gcgcgatatc agaggctacg gaagcgatta tcacagattg tacggacagg aaattgtatt
     3121 tgaattcggg gcgcatctcc tcgaatacga aaagaatgcc ggctagaggc gcgttgaatg
     3181 ccgcggttaa tccgccccca gcgccggccg cgatgagcgc atgggtgtct tccctgtcgg
     3241 ccctgaagat atccgcgacc atctttccta tattcccgcc catctgtatc gtcggcccct
     3301 ctcttccgag caccatgccg ccgccgaggg agcagagacc gcccaggaat ttcaccggca
     3361 atacgttctt ccacctaatc ggtcttacgt cgtcaagcgc cccttcgatc tcgtggaccc
     3421 cgcttccgct cgcctcaggg gccaatctcc gtacgagaag aaaggcgatg taaactagaa
     3481 cggccgagaa aagtactgaa gcggctaact ggaacgcgga actctgagga aaatttcgca
     3541 gaaggagctc tctcgagtcc tggacttttt ctatagagag ctgaaagacc gcgcctgcca
     3601 gtcctgtgag cgcgcctact atgagggacc a
//
LOCUS       DASSJH010000003         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_757724, whole genome shotgun sequence.
ACCESSION   DASSJH010000003 DASSJH010000000
VERSION     DASSJH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757724"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..458)
                     /locus_tag="VFJ67_00060"
                     /pseudo
     CDS             complement(1..458)
                     /locus_tag="VFJ67_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032454.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(570..1925)
                     /locus_tag="VFJ67_00065"
     CDS             complement(570..1925)
                     /locus_tag="VFJ67_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7288801.1"
                     /translation="MQKILVIDDEKGIRDSLSMIFGIEGYEAVSLDNAKAGLSLIDEG
                     ERYDFIICDIKLPGMGGMEFLEEIRERDLAPVIIMISAYGTVESSIEAIKKGADDYIN
                     KPIITEELILRMRMAEERRRLRQRTVYLEKEIGTRGEFGDIIYVSSQMEEVIRLAEKA
                     SQYKTTVLISGESGTGKELIAKAIHNGSPRRDAPFMAVNCAAIPETLLESELFGYAKG
                     AFSGADSSKRGLIEEAHGGTLFLDEIGEFPLTLQPKLLRVLQEGEIRRLGDTRLIKTD
                     LKIIAATSRDLARETERGRFRNDLYYRLNVLPVTIPPLRERKEDVPPLVRYFIAKHNR
                     RLNRRITDATPEAMSEIMSYDWPGNVRELENAVERAMILSDSEYIQKIDIGATLPKRG
                     PSPESWIETLTYEEARHRIEKSYIEKALAESRGNRTKAARILGISRRSLLYKLKEQKE
                     G"
     gene            complement(1903..>2830)
                     /locus_tag="VFJ67_00070"
     CDS             complement(1903..>2830)
                     /locus_tag="VFJ67_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7288802.1"
                     /translation="RPVHELINTTEKIGAGRFPESAELGNVKEINQLYSALRAMYGEI
                     EDGKRKLRDKIMELEETNAELRLTQKELIATEKLASLGQLSAGIAHEIGNPLSAIKGY
                     TEVLRRASGMDDAKRGEILGDILREVSRVDRIIRTLLDYSRPRKSSPQIVDVNEVIKD
                     TAEIIMSQGALKGIGLTLELSEDTPQIIADPGQLSQVIVNLLLNSRDAVEDGGEIVIS
                     TARDEDSGAVICVRDDGEGIPEEIIDRIFDPFFTTKDPGHGTGLGLSVSARIVETFEG
                     VIEVESSEGSGALFRIIFPAARGYRNAENFGN"
BASE COUNT          486 a          898 c          665 g          781 t
ORIGIN      
        1 gtctttatgt cgatcttgtc catctgaaga agcgccttca tgactctctt tgatttcccg
       61 gcgtccttgt cctggagcat cttgcctaaa gcgacgggaa cgatttgcca cgaaacgccg
      121 tacttgtctc tgagccagcc gcaacgttgt ttttctccgc cttcggacag cttttcccag
      181 aacccattca cctcttcctg cgtctcgcag ttgacgacga acgatatcgc gggcgagaaa
      241 gtgaaatacg gcccaccgtt caacgccacg aatttttgtc cttcgagctc gaattccacg
      301 gtcattacgg ttcccttcgg ttgtcccgag acctcggccc cttcttctcc ttaacaggcg
      361 acgctcccga tcttcgaatt cttaaaaacg gaagtgtaaa agttcgccgc ctcttcggcc
      421 tggttatcga accacaggaa cggtgttatc ttttgcatgc gatttttccc ctgattcctg
      481 aattgtaaag taagctatag attaccacgg tatcctctaa ggcggccgtc gtatgctccg
      541 gcctttgtgg ttcatactga aggcgagcct cagccctcct tctgctcctt cagtttatag
      601 agcagagagc ggcggcttat tcccagtatc ctggccgcct tcgtgcggtt gccgcgggac
      661 tcggcgaggg ccttctcgat gtatgacttt tcgatcctgt gcctagcctc ttcatacgtc
      721 agcgtttcga tccaggattc cgggctcggc ccccgtttcg gcagtgtcgc tccgatgtct
      781 attttctgta tatattccga atccgagagt atcatcgccc tctcgacggc gttctcgagc
      841 tccctcacgt tgccgggcca gtcgtaactc attatctccg acatcgcctc gggcgtcgcg
      901 tccgtaattc gccgattcag ccttctgtta tgtttggcga taaaatagcg tacgagcggg
      961 gggacgtctt ccttcctttc cctgaggggc ggtatggtaa cggggagcac gttcagcctg
     1021 tagtataggt cgttcctgaa cctcccgcgc tcggtctcgc gtgcgaggtc ccgtgacgtg
     1081 gcggctataa tcttgagatc ggtctttatg agcctggtat ccccgagcct ccttatctca
     1141 ccctcctgaa gcacacgcag cagtttgggt tgaagcgtca gcggaaactc cccgatctcg
     1201 tccaggaaca gtgtgccccc gtgggcttcc tcgatcagtc cccttttcga gctgtcggcg
     1261 ccgctgaacg cccccttagc gtatccgaag agctcgctct caaggagcgt ttccggtatg
     1321 gcggcgcaat tgaccgccat gaacggcgcg tccctcctgg ggctgccgtt gtgtattgcc
     1381 ttggctataa gctcctttcc cgtgccgctc tcgcccgata tcaggacggt ggtcttgtac
     1441 tggctggcct tttccgcaag ccgtatcacc tcttccattt gactgctcac atagattatg
     1501 tccccgaact ctcccctcgt gccgatctcc ttctcgaggt acaccgtcct ctgcctgagc
     1561 cgccgtctct cttccgccat cctcatcctg agaatgagct cttccgtaat tatcggtttg
     1621 ttgatatagt cgtcggctcc ctttttaatt gcttcgatgg agctttcgac ggtcccgtac
     1681 gcggagatca ttattatgac gggcgccaaa tccctctccc gtatctcttc cagaaactcc
     1741 atccctccca ttcccggaag ctttatgtcg catattatga aatcgtatct ttcgccctcg
     1801 tctatcagtg agagcccggc cttcgcgtta tccaagctca cggcttcgta gccttcgatc
     1861 ccgaatatca tcgaaagcga gtctcttatt cctttctcgt cgtcaattac caaaattttc
     1921 tgcattccga taacctcttg cggccgggaa aattattctg aacagcgctc cgctcccttc
     1981 cgagctttcg acctcgatca cgccttcgaa cgtctcaacg atcctggccg agaccgagag
     2041 gccgagcccc gtcccgtgtc ccgggtcctt ggtcgtaaag aaaggatcga aaatcctgtc
     2101 gattatttct tccggtatgc cctcaccgtc gtctctcacg cagatgacgg cgcctgaatc
     2161 ttcgtcccgc gcggtcgaga ttacgatctc ccccccgtcc tccacagcgt ccctggagtt
     2221 aagcaggagg ttcacgatca cctgcgagag ctgtcccggg tcggctatta tctgaggcgt
     2281 atcctccgat agctcgagcg tgagcccgat gccctttaac gcgccctggc tcattattat
     2341 ctcggccgta tcctttatga cttcgttcac gtcgactatc tggggggagc tctttcttgg
     2401 acgcgaataa tccagcagcg tcctgataat tctatccacg cggctcacct ctctcagtat
     2461 gtcgccgagt atctcgcctc tcttcgcgtc gtccatgccg gacgccctcc tgagcacttc
     2521 cgtgtacccc ttgatagcag aaagagggtt ccctatctcg tgtgctatgc ctgccgagag
     2581 ctgcccgagt gaagcgagct tctctgtcgc gatcagctcc ttctgcgtca acctgagctc
     2641 cgcgttcgtt tcctcgagct ccattatctt gtccctgagt ttcctttttc cgtcttctat
     2701 ctccccgtac atagccctga gcgccgaata tagctggttg atctccttga cattccctaa
     2761 ctcggccgac tccgggaacc tccccgcgcc tattttctcc gtcgtattta taagctcgtg
     2821 cacgggcctg
//
LOCUS       DASSJH010000004         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_433276, whole genome shotgun sequence.
ACCESSION   DASSJH010000004 DASSJH010000000
VERSION     DASSJH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433276"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFJ67_00075"
     CDS             <1..679
                     /locus_tag="VFJ67_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET7288803.1"
                     /translation="ANGSGVVGRHYMGHVNSILMALSKKPNPTIFQKTLALNDFYFGS
                     GDYEFPMGHISFVGKLDAATLSAGAPAIVPGMTLDIMAKHSLDFWITSEDLPDPENRV
                     TLDSKGNIVLSYTPNNQESHKRLIAKLKGLMNTIGCHEEHLIPRNLFIGQQIPLAGVA
                     HQNGTIRFGNDPASSALDADCRAHEVDNLYVVDASFFPSCGAVNPALTIMANSLRVGD
                     RILGRLG"
     gene            723..1778
                     /locus_tag="VFJ67_00080"
     CDS             723..1778
                     /locus_tag="VFJ67_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7288804.1"
                     /translation="MNKMNTGHTKPIRYSLYILVILLVTLTGAGFGSAEDRASVHAVD
                     AVGMTVSDMDRSVAFYADVLGFEKISDVEVLGDGYEHLHGIFGLRMRVVRMQLGDETI
                     ELTEYLTPKGRPFPADTRSNDRWFQHIAIVVSDMDRAFERLRSNKVRYASTAPQTIPA
                     WNKAAAGIRAFYFRDPDGHFLEIIYFPEGKGNPKWRRNSNDDKLFLGIDHTAIVVNDT
                     EESLKFYKDALGMHVAGESMNYGTEQEHLNSVFGAVLRITSLKAGQGPGIEFLEYLAP
                     GDGRPAPADERSNDIQHWQTTLDSDDPNGLAERLKDDGYSLISAETVELPGGELGFTK
                     GFLVKDPDGHVMQVIEK"
     gene            1800..2366
                     /locus_tag="VFJ67_00085"
     CDS             1800..2366
                     /locus_tag="VFJ67_00085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET7288805.1"
                     /translation="MGSERPLFIPVILGTTRKGRMSKHVANFMAGEIAKQEGIETEVV
                     DILDLQLPFNDAGEQIKNEGFSATAMKADAFVIVAPEYNHGYPGPLKHALDSNLKEYI
                     HKAVGICGVSAGPFGGARVIENLLPVMRELGLVTIFWDVNFSLVQKTFDESDRLLDEA
                     FVKRAEKFIKELVWMAKVLRYGRDNVEI"
     gene            2411..2587
                     /locus_tag="VFJ67_00090"
     CDS             2411..2587
                     /locus_tag="VFJ67_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288806.1"
                     /translation="MPKKELKCPRCGAEMNNHAEKISYETDSGGHRPDPDFGGIVEDV
                     YACPGCGAIEMRPT"
BASE COUNT          733 a          713 c          793 g          532 t
ORIGIN      
        1 cgccaacggg tcgggtgtcg tcgggaggca ttatatgggg cacgtgaact cgatactcat
       61 ggcactctcc aagaaaccaa atccgacgat attccagaag acgctcgcgc ttaacgactt
      121 ttatttcggc tccggggatt acgaattccc gatgggccac atttcattcg tcgggaagct
      181 cgacgcggca actctgtcgg cgggagcgcc cgctatcgtt ccgggcatga cgctcgatat
      241 tatggccaag cactccctcg atttctggat cacgtctgaa gacctgcccg atcccgaaaa
      301 cagggtcacc ctcgactcga aagggaatat tgtcctttcc tacacgccca acaaccagga
      361 gtcgcacaag cgcctcatcg cgaagctcaa aggcctcatg aacactatcg ggtgccacga
      421 ggaacatctg atccccagga acctgttcat cgggcagcag ataccgctcg cgggcgtcgc
      481 gcaccagaat ggaacgatac gattcggcaa cgaccccgcg tcctctgcac tcgacgccga
      541 ctgcagggcg cacgaggtcg ataacctcta cgtagtggac gcgagcttct tcccttcctg
      601 cggcgcagtg aatcccgcac tcactataat ggccaactcg ctccgtgtag gtgaccggat
      661 actcgggaga ctcggatgat accggggaaa atcatttcaa tgcggaattc agggacaaaa
      721 ccgtgaataa aatgaatacg ggacacacaa agcccatcag atacagttta tatatactgg
      781 tcatcctgct cgtcactctc acgggtgccg gattcggttc ggcagaggac agggcctccg
      841 tgcacgcggt cgacgcggtg ggaatgacgg tctcagatat ggacagatct gtcgctttct
      901 acgctgacgt gctcggcttc gaaaagatat ccgacgtaga agtgctcggg gacggttacg
      961 agcacctgca tgggatattc gggctcagga tgagggtagt gcgcatgcag ctcggagatg
     1021 aaacgataga gctgaccgag tacctgactc ccaaaggcag accgttcccc gccgataccc
     1081 ggagcaacga ccgatggttc cagcacattg cgatcgtcgt gagcgatatg gatagggctt
     1141 tcgagcgcct gcgcagcaat aaagtgcgtt acgcttccac ggcgcctcaa accatacccg
     1201 cgtggaataa ggcggccgcg ggaataagag cattttattt cagggacccg gacggccatt
     1261 ttctcgaaat catttatttc cccgagggca aggggaaccc caaatggcgg cgtaatagta
     1321 atgatgataa acttttttta ggcattgacc atacggctat agtagtgaat gatacggaag
     1381 agagcctgaa gttctacaag gacgcactcg gcatgcacgt agcgggcgag agcatgaatt
     1441 acgggaccga gcaggagcac ctgaatagcg tattcggcgc ggtgctccgc atcacgtcat
     1501 taaaggccgg acagggaccg ggaatagagt tcctcgaata ccttgcgccc ggcgacggaa
     1561 ggcccgctcc agccgatgag agatcgaacg atatacagca ctggcagact acgctcgatt
     1621 cggacgatcc gaacgggctt gcggaaagac tgaaagacga tggttattcc ttaatatctg
     1681 cggaaacggt tgagttaccc ggcggagagc tcggttttac gaagggtttt ctcgtgaagg
     1741 accccgacgg tcacgttatg caggtcatag aaaaataatt agagtccaag gagccgaaga
     1801 tgggatcaga gagacctcta ttcatacccg taattctggg tacgacgaga aaggggcgca
     1861 tgagcaagca tgtggccaat ttcatggccg gcgagatcgc gaagcaggag gggatcgaga
     1921 cggaggtcgt cgacatactc gatttacaac ttcccttcaa cgatgcgggc gagcagataa
     1981 agaacgaggg cttttccgca acggcgatga aagcggacgc gttcgtcata gttgcccccg
     2041 agtacaacca cggctatccg ggacccctta agcacgcgct cgactccaac ctcaaggaat
     2101 atatacataa agcggtcggc atctgcggtg tttcagcggg accgttcggc ggcgcgaggg
     2161 tgatcgagaa cctgcttccg gtgatgagag agctcgggct cgtcaccata ttctgggacg
     2221 tcaatttctc gctcgtgcag aaaacgttcg atgagtcgga cagactcctc gacgaagcgt
     2281 tcgtaaaacg cgcggaaaaa ttcataaagg agctcgtatg gatggcgaaa gtgctcagat
     2341 acggacgcga caacgtggaa atatgaggat catgacaata tcgagtgagg tatgacgaag
     2401 gagaatatac atgcctaaga aagaattgaa atgccccagg tgcggagccg agatgaataa
     2461 ccacgcagag aaaataagct acgaaacgga tagtggcggt cacaggccgg acccggactt
     2521 cggtggaatc gtcgaagacg tgtatgcatg cccgggatgc ggcgccatag agatgagacc
     2581 gacctaatga agccggcctt caagctcatt gcgtacagca gttgagaata actaccggtt
     2641 tagaatctaa tcacggatac cggaggagac gcacaatggc cgacataaaa aaggcaagga
     2701 caacttcagt ccgaaagaga cccgggaagc acgatggcaa tctcgaggaa gccatagcga
     2761 gaaaggctta c
//
LOCUS       DASSJH010000005         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_865874, whole genome shotgun sequence.
ACCESSION   DASSJH010000005 DASSJH010000000
VERSION     DASSJH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865874"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VFJ67_00095"
     CDS             <1..965
                     /locus_tag="VFJ67_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7288807.1"
                     /translation="MEDAREQFEKAERMDVENPDLMLRLGLLYIEGGEYDKAMEEFRL
                     ILAGNPGDDRAKYYLAVCYIETGQYDTAISMLDTIPQYSEFYDESLVQKAYIFEKRNM
                     LPRSLALMEDVYSREPNNEVIVNYLGSVYRKSGRNEDAVNLYKKFLEANPKSETIYYS
                     LGVTYYLIDREQDSIDTMRKLIEINPKHSDALNFVGYSYAEKGENLDEAEALIRKALA
                     LSPNKGYILDSLGWVYYKKRNYPEAIRLLKQAAALQSDDPAILEHLGDAYRDMGDTAS
                     ALEYYNKGLKMLYEVQTDNPEDIELRDRLRQKVDQLSKNPKIQS"
     gene            968..1762
                     /locus_tag="VFJ67_00100"
     CDS             968..1762
                     /locus_tag="VFJ67_00100"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HET7288808.1"
                     /translation="MKQYRNALYIALILLASACAPRVAEVDMTAADPGRIIEKIELLE
                     SGVRTVKGLARVRIATAADKVSYTQVTVAERPNLIRLEALNPFGSTVGFISSDGTHIY
                     IISSGERGVYDAGEEFDLSYVYPGLDIRITAESLVNLVTGRLPYSIYESPVKPSVSAD
                     SGMIKLTWSGGNAGGDNALWVNRDNYRVEKAEFTLANGERASVLYEYFDGLVDGHYFP
                     RKIDFDTGGLSIAIVYEPGVKLNGGVDRSLFIPTAMIEKSSGQNYN"
     gene            1841..2125
                     /locus_tag="VFJ67_00105"
     CDS             1841..2125
                     /locus_tag="VFJ67_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET7288809.1"
                     /translation="MPETEVVRVFTIKNKLGLHARAAAVFVRLSSRFLCDVKIVKNGY
                     EVDGKSILGVLSLAAAKGTDIKIITKGEGSEQAMNEIEELIESGFGEGVL"
     gene            2122..>3322
                     /locus_tag="VFJ67_00110"
     CDS             2122..>3322
                     /locus_tag="VFJ67_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET7288810.1"
                     /translation="MRVERKGIPISAGIALGKVALLDRSKMIVERVQVEEHLISAEKE
                     RFLQAVKRSKDQLLSIRDKLEPLEAGDHLQILNLNIMMLEDELLSDEVLRFIESEHVN
                     AEWAVNHILSIKSEAFRKVEDQYMKERLADIYYMGQRILRNLHGVAEDIPDLGHDSIL
                     VAHEISAVDVVAFAKHRAIGIATDTGASTSHSAIVAKSLGIPAVMGLEDITLRASPGD
                     AIYIDGFRGIVILDPAPGEQEEFKRRKSSYIALEKKLLEYAKLPGRTLDGRNIKVNAN
                     IEIIEELTHAVCYGAEGIGMYRTEFLFTKSDAFPGEEEQYENYMKVVSALDSPLVTIR
                     TLDIGGDKFPSGMEQAQRLNPALGLRGIRFSLRDEETFRTQIRAILRTAGEKKVRILI
                     PMVSDMAE"
BASE COUNT          854 a          890 c         1004 g          574 t
ORIGIN      
        1 ggatggagga cgcgagggaa cagttcgaga aggcggaaag gatggacgtc gagaaccccg
       61 acttgatgct ccggctcggg ctcctctaca tcgagggcgg ggagtacgat aaagctatgg
      121 aggaattcag gctcatcctc gcgggcaacc ccggagacga ccgcgcgaaa tattatctcg
      181 ccgtctgcta catagagacc ggacagtacg atacggccat aagcatgctc gataccatac
      241 cgcagtactc ggagttctac gacgagagcc tcgtgcagaa ggcatacata ttcgagaagc
      301 ggaacatgct cccccgctcg ctcgctttaa tggaagacgt ctacagccgg gagcccaaca
      361 acgaggtgat agtaaactat ctcggcagcg tttacaggaa gtcgggcagg aacgaggacg
      421 ccgtgaacct gtacaagaag ttcctcgaag cgaacccgaa gagcgaaacc atatactact
      481 cgctcggcgt tacgtattat ctcatcgacc gggagcagga ttccatagac accatgagga
      541 agctcataga gataaacccg aaacactcgg acgcccttaa cttcgtcggt tactcctacg
      601 ccgagaaggg ggagaacctc gacgaggccg aggcgctcat aaggaaagcg ctcgcccttt
      661 cgcccaataa ggggtatata ctcgacagcc tcggctgggt gtattacaag aagagaaatt
      721 acccggaggc gatcaggctc ctcaagcagg cggcggcgct ccagtcggac gatcccgcca
      781 tattggagca cctgggggac gcttacaggg acatgggcga cacggcatcc gcgctcgaat
      841 attacaacaa gggcctcaag atgctctacg aggtgcagac cgataacccc gaagacatag
      901 agctccggga caggctccgt caaaaggtcg accagctctc gaaaaacccg aagatacaaa
      961 gctgaaattg aaacagtacc gcaacgcatt atacatagcc ctgattctgc tcgcgtcggc
     1021 atgcgcgccg agggtggccg aggtcgatat gacagccgcc gacccgggaa ggattatcga
     1081 aaagatagag ctcctcgaga gcggggtgcg caccgtgaag ggactcgcaa gggtcaggat
     1141 agcgacggcc gcggacaagg tctcctacac gcaggtcacg gtcgccgaga gacccaacct
     1201 cataaggctc gaggcattga accccttcgg gagcacggtc gggttcatct cttccgacgg
     1261 cacgcacatt tacataatat cctcgggtga aaggggcgtt tacgacgcgg gcgaggaatt
     1321 cgacctctct tacgtctatc cgggcctcga cataaggata acggccgaga gcctggtcaa
     1381 cctcgtcacg ggacggctcc cctacagcat ctacgaatcc cccgtaaaac cctccgtatc
     1441 cgccgactcg ggtatgataa agctcacgtg gagcggcgga aacgcgggcg gagacaacgc
     1501 gctctgggtc aacagggaca actatagggt ggagaaagcc gagttcacgc tcgctaacgg
     1561 cgagagggcg agcgtcctct acgaatattt cgacgggctc gtcgacgggc attatttccc
     1621 gcgcaagata gacttcgaca cgggcgggct ttccatagcg atagtgtacg agccgggcgt
     1681 caagctcaac gggggcgtcg acaggtctct cttcattccg acggccatga ttgaaaaatc
     1741 ctccgggcaa aattataatt aagccgggat gacaaaacgc gctcacgctc gtccgggtac
     1801 agcagaaacc aagtcataga tcggttgagg ttatccagag gtgccggaaa cggaagtagt
     1861 cagggttttc accataaaaa acaagctggg tcttcacgcg cgcgctgccg ccgttttcgt
     1921 aaggctgtcg agcaggttct tatgcgacgt gaagatcgtt aagaacggct acgaggttga
     1981 cggtaaaagc atcctcggcg tgctttctct cgctgcagcg aagggcaccg acataaagat
     2041 aatcacgaag ggagagggtt cggagcaggc gatgaacgaa atagaagagc tcatcgaaag
     2101 cgggttcggc gagggcgtat tatgagggta gagcggaagg gcatccccat atccgccggg
     2161 atcgcgctag gaaaggtggc gctcctcgac agatcgaaga tgatcgtcga gagggtgcag
     2221 gtcgaagagc acctcatcag cgccgagaag gagaggttcc tccaggcggt caaacggtcg
     2281 aaggatcagc tcctgtccat cagggacaag ctcgagccgc ttgaggccgg ggaccacctc
     2341 cagatactta acctcaatat catgatgctc gaggacgagc tgctctcgga cgaggtgctc
     2401 aggttcatag agtccgagca cgtgaacgcc gagtgggccg taaaccacat cctttcgata
     2461 aagagcgagg ctttcaggaa ggtcgaggac cagtacatga aggaaaggct cgccgatatc
     2521 tattacatgg gccagaggat actccggaac ctccacggcg tcgccgagga catacccgac
     2581 ctcgggcacg attccattct cgtcgcgcac gaaatatcgg ctgtggacgt ggtcgcgttc
     2641 gcgaagcaca gggccatcgg tattgccacg gatacgggcg catcgacctc ccacagcgct
     2701 attgtggcaa agtcgctcgg cataccggcg gtcatgggtc tcgaggacat cacgctcagg
     2761 gcgagccccg gggacgcgat atacatagac gggttcaggg gcatcgttat actcgatccc
     2821 gcgcccggag agcaggagga gttcaaacgc cggaagtcga gctacatcgc gctcgaaaag
     2881 aagctgcttg agtacgcgaa gctccccgga aggactctcg acgggaggaa cataaaggtc
     2941 aacgcgaaca tagagatcat cgaagagctg acgcacgccg tctgttacgg ggccgaaggg
     3001 ataggcatgt accggacgga attccttttc acgaagtcgg atgcgtttcc cggagaagaa
     3061 gaacagtacg aaaactacat gaaggtggtg tcggcgctcg attcgcctct cgtcacgata
     3121 aggaccctcg acataggggg agataaattc ccgtccggca tggagcaggc gcagaggctg
     3181 aaccccgcat tgggcctccg gggaataagg ttctcgctca gggacgagga gacgttcagg
     3241 acgcagataa gggccatact ccggacggcg ggggagaaga aagtaaggat actcattccg
     3301 atggtatcgg acatggccga aa
//
LOCUS       DASSJH010000006         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_434393, whole genome shotgun sequence.
ACCESSION   DASSJH010000006 DASSJH010000000
VERSION     DASSJH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434393"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /gene="mgtE"
                     /locus_tag="VFJ67_00115"
     CDS             complement(<1..1207)
                     /gene="mgtE"
                     /locus_tag="VFJ67_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET7288811.1"
                     /translation="MEGTPLEFYVKKFSEYDPVISAHVIETLDAHDSAEVLKSLPAEV
                     SKTIFENLQNEYAALLLGELPDELAGEIIRGMEPLKVSLIVSVCPMALKNKIATNLTE
                     KQKREIGELLEYPKDSVGYIMSPLFLALRDELRISEAVNKIKSMAGQEHMPLSYVYVV
                     DADSRLTGVLSMRDLLIRDPHKKLSEVMIKNVFYLNAFMNAEDAASIVSKQKFFAVPV
                     VDNTNKLVGIVNTSQLLSDMQEEAATDIQTIFGVSSEERPFSSTFFSLKKRLPWLNIN
                     LATAFLAGGVVAVFESTIAKLTVLAIFLPIVAGQAGNGGAQSLAVVMRGLVMREISMR
                     DFPKLLWKEMLLGLINGVAIGIVTAVIAYIWKGDPTLGLVVCLAMIINLIIAGLSGAV
                     IPIGLKSFGL"
     gene            complement(1367..2053)
                     /locus_tag="VFJ67_00120"
     CDS             complement(1367..2053)
                     /locus_tag="VFJ67_00120"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7288812.1"
                     /translation="MHTRSPKQPPAKRSYGLASEEWIDITVPLSDGMVNWPGDPPVKV
                     FRVRDMGKGDEFNMTALSMSVHTATHLDAPSHFTKLKTGIDKMPLSAVVGNARVIEIE
                     DKELITPEELRPHKLKKGERILFKTRNSKRRWDKKPFNENFVHLSTDAAKYLVERQVQ
                     TIGVDYLSIGGYEGNVSEVHHIILNARIWVIEGLDLSQIEPGNYELICLPIKIENCEG
                     APARALLRPV"
     gene            complement(2155..2682)
                     /locus_tag="VFJ67_00125"
     CDS             complement(2155..2682)
                     /locus_tag="VFJ67_00125"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET7288813.1"
                     /translation="MSPKKSSKPKNIFLVGFMGAGKTTVGRILARRLGWRYCDADKVI
                     ETVAGKSVADIFSGRGEEYFRQLESETLRDLAGKERQVVATGGGAVMREENMRAMKGG
                     GVTVYLKAPMSVIWERIKHSKTRPLLNVENPLEAAGELLNKRIPFYEAADVTVDTESL
                     TPEEAAGEIIKRLGI"
     gene            complement(2690..3304)
                     /locus_tag="VFJ67_00130"
     CDS             complement(2690..3304)
                     /locus_tag="VFJ67_00130"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET7288814.1"
                     /translation="MFTGIVEDIGTVAAVKRRTKESTITFRVRKIDPRGIVTGESISV
                     SGACLTVTSVERDTFTVDASRETLSRTSLRKLRAGQAVNLERSLRAGDRMGGHIVSGH
                     VDGVGKVKAKKKRGGSVEFTFTAPESVLKYIVEKGSVAVDGVSLTVNSVKSGEFTVNI
                     IPYTLAETTFGSLRTGSPVNIECDIIGKYVEKLMTGKRDEYTVY"
     gene            complement(3307..3792)
                     /gene="lspA"
                     /locus_tag="VFJ67_00135"
     CDS             complement(3307..3792)
                     /gene="lspA"
                     /locus_tag="VFJ67_00135"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7288815.1"
                     /translation="MHKYRTAAIITVLVVVIDQISKWLIITSVPLYGKINLLPFLDVT
                     HIRNPGAAFGIFRELPESLRLPLFILVLIAAVVVIIYFLSKTGNNDRLLVVSLSLILG
                     GAIGNSIDRFRLRYVTDFIDFHWFGNPSLHWPPFNLSDSAITIGVILILFDTFILKRG
                     R"
     gene            complement(3827..>4265)
                     /locus_tag="VFJ67_00140"
     CDS             complement(3827..>4265)
                     /locus_tag="VFJ67_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7288816.1"
                     /translation="LPPDLRREIDVKLSAVGTGGTPGETVREFFSTRDKALQTVSLSE
                     SLLNSPDYAPPYYLQGRLLFNMGEYERAVPYLSMAEYLGLPSDELTRENLRILGISLF
                     ASGRYDESDIIWSRLVSDGDPESMAYAGDFIQRTGWARKNRLK"
BASE COUNT          826 a         1242 c         1186 g         1011 t
ORIGIN      
        1 cgagaccgaa cgacttgagg ccgatgggta tgaccgcacc cgagaggcca gcgattatca
       61 agttgataat catggcgaga cagacgacca gcccgagcgt cgggtcccct ttccagatat
      121 acgcgatcac ggcggttact atgcctatgg cgaccccgtt gatgagaccg agcagcatct
      181 ctttccagag gagcttcggg aagtctctca tcgatatctc tcgcatgacg agccccctca
      241 tcacgacggc gagcgactgc gccccgccgt tccctgcctg gcccgcgacg atggggagga
      301 atatggcgag caccgtgagc tttgcgatcg tgctctcgaa gaccgcgaca accccgcccg
      361 cgaggaatgc cgtcgcgagg tttatgttca gccaggggag tctctttttg agggagaaga
      421 acgtgctcga aaatggcctc tcctcgctcg atacgccgaa tattgtctgt atatcggtcg
      481 cggcctcttc ctgcatgtcg ctcaggagct ggctcgtgtt cactatgccg acgagcttgt
      541 tagtgttgtc caccacgggg acggcgaaga acttctgctt cgacactatg ctggccgcgt
      601 cctcggcgtt catgaaagcg ttcagataaa agacgttctt aatcatgacc tccgagagct
      661 tcttgtgagg gtcccttatg aggaggtccc tcatgctgag gacgcccgtg agcctggagt
      721 ccgcatccac gacgtaaacg taggaaagcg gcatgtgctc ctgcccggcc atcgacttta
      781 tcttgttcac ggcttccgaa atcctgagct cgtctctcag cgcgaggaac agcgggctca
      841 ttatgtatcc gacgctgtcc ttcgggtatt ccagcagctc ccctatctcg cgcttctgct
      901 tctcggtgag gttcgtcgct atcttgttct tgagcgccat cgggcagacg ctcacgatga
      961 gcgatacctt gaggggctcc atgccgcgta tgatctcccc ggcgagctcg tccggcagct
     1021 cgcccaggag gagagccgcg tactcgttct ggaggttctc gaatatagtc ttcgacacct
     1081 ccgccgggag ggacttcaga acctctgcac tatcgtgcgc gtcgagggtc tctataacgt
     1141 gcgccgagat aacggggtcg tattccgaga atttctttac gtagaattca agcggtgtgc
     1201 cttccataat tctggacctc cgtgtattga ccggccttga agcctggata tacaagaata
     1261 cataacggag aggggttatg gtaacgggac gctcggccgg ggaggctctg ttaatgctcg
     1321 tttaattaga tcgcgccggg atggcgctcc tacggttctt aattaatcaa accggcctca
     1381 gcaatgcccg ggcgggcgcg ccttcgcagt tctctatctt gatgggcagg catatgagct
     1441 cgtagttccc gggctcgatc tgcgagaggt cgagtccctc gatgacccat atgcgcgcgt
     1501 tcaaaataat gtggtgcacc tccgatacgt tcccttcgta tccgccgatt gagaggtaat
     1561 cgacacctat cgtctgcacc tgccgctcga ccaggtactt cgcggcgtca gtcgacaggt
     1621 ggacgaagtt ctcgttgaaa ggcttcttgt cccatctcct cttcgagttc ctcgttttga
     1681 aaagaattcg ctcgcccttc tttaacttgt ggggcctgag ctcttcgggg gttatcagct
     1741 ccttgtcctc gatctctatc acgcgcgcgt tgccgacgac ggccgagagc ggcatcttgt
     1801 cgatccctgt cttgagcttc gtgaaatggg atggggcgtc cagatgcgtc gccgtgtgca
     1861 cggacatcga gagcgcggtc atgttgaatt cgtccccctt tcccatgtcg cgcacgcgga
     1921 atacctttac cggagggtcg cccggccagt tgaccatccc gtccgagagc ggcacggtta
     1981 tgtctatcca ttcctcggag gcgaggccgt agcttctttt cgccggaggt tgtttagggg
     2041 agcgtgtatg catagccaat taatgttaaa caatatttcg gggaaggaca aacgagattg
     2101 ccgcgcggag cctgtcctga gtttatcgaa gggctcgcaa ttacaatgcc cctctcaaat
     2161 cccgagtctt ttaattatct ccccggcggc ttcctcggga gtgagggact cggtatcgac
     2221 ggttacgtcg gccgcttcat aaaaggggat acgtttatta agcagctctc cggcagcttc
     2281 gaggggattt tccacgttga ggagtggacg cgttttactg tgctttatcc tctcccagat
     2341 gacgctcatc ggggccttca ggtaaacggt caccccgccc cctttcattg cccgcatgtt
     2401 ctcttccctc atcaccgctc ctcccccggt cgccaccacc tgtctttcct tcccggcgag
     2461 gtcccggagc gtctcggatt cgagctgcct gaaatattcc tcgccgcggc cggagaatat
     2521 atcagcgacg gacttcccgg ccactgtctc tatgaccttg tcagcgtcgc agtacctcca
     2581 cccgagcctg cgggcgagta tcctgcccac ggtggtcttt ccggctccca tgaagcccac
     2641 gaggaatatg tttttcggct tgctgctttt tttgggactc atctcggaat taatagaccg
     2701 tgtattcgtc tcttttgccc gtcatgagct tttccacgta cttcccgatt atatcgcact
     2761 cgatgttgac ggggctccct gtcctgagag agccgaacgt agtctcggcg agcgtgtagg
     2821 ggatgatgtt caccgtgaat tctccgcttt taacggagtt cacggtcagg ctcacgccgt
     2881 ccacggcaac cgagcccttc tccactatgt atttcagcac gctctcgggc gctgtgaacg
     2941 tgaactcgac agagcctccc cgcttctttt tcgcctttac cttccccacg ccgtcaacgt
     3001 ggccgctcac gatgtgcccg cccatcctgt ccccggccct gagagagcgc tcgaggttga
     3061 cagcttggcc cgccctcagc ttccggaggc tcgtcctcga aagcgtctcc cgcgacgcat
     3121 cgacggtgaa cgtgtccctc tcgacggacg ttaccgtgag gcacgcgccg cttaccgata
     3181 tgctctcgcc ggttacgatc ccgcgcgggt cgatcttcct cacgcggaac gtgatcgtcg
     3241 attccttcgt gcggcgtttt acggcggcga cagtccctat gtcctctact atccccgtga
     3301 acatcgctat cttccccgct tgagtataaa cgtgtcgaaa agtattagta taactcctat
     3361 ggttatcgcc gagtcagaca ggttgaaagg aggccagtgg agggaggggt taccgaacca
     3421 gtggaagtcg atgaagtctg tcacgtagcg gagcctgaac ctgtctatcg aattccctat
     3481 agcaccgcct agaatgagcg aaagcgagac gacgaggagc ctatcgttat tcccggtctt
     3541 ggagagaaaa tagataatga cgaccacggc cgcaatcagc acgagtatga acagcgggag
     3601 cctgagagac tcgggcagct ccctgaatat gccgaaggcc gccccggggt tcctgatgtg
     3661 cgtaacgtcg aggaacggga gcaggtttat cttcccgtaa agcggcacgc tcgtaatgat
     3721 gagccacttg gaaatctggt ctataacgac gaccagcacg gtgataattg cagctgttct
     3781 gtatttgtgc attttacgga agctaagatt atataggatt cgaagattac ttcaaccggt
     3841 ttttccgggc ccagccggtc ctttggatga aatcgccggc gtaggccatc gattcggggt
     3901 ctccgtccga gacgagacgg ctccatatta tgtccgactc gtcgtacctg ccggaggcga
     3961 aaagggaaat gccgagtatg cggaggttct cgcgcgtgag ctcgtccgag gggaggccca
     4021 ggtactcggc catagagaga tacggcaccg ccctctcgta ctctcccatg ttgaagagaa
     4081 gcctgccctg gaggtaatag ggcggagcgt aatcagggga attcaataac gactccgata
     4141 acgagaccgt ctgaagcgct ttgtccctgg tcgagaagaa ctccctcacg gtttcgcccg
     4201 gagtgccgcc cgtcccgacc gcggagagct tcacgtcgat ctccctcctg aggtcaggtg
     4261 ggaga
//
LOCUS       DASSJH010000007         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_975826, whole genome shotgun sequence.
ACCESSION   DASSJH010000007 DASSJH010000000
VERSION     DASSJH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975826"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFJ67_00145"
     CDS             complement(<1..528)
                     /locus_tag="VFJ67_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HET7288817.1"
                     /translation="MSRIKDLSKVGQSIWYDYIRRSFITSGELQALIEEGLRGETSNP
                     SILEKAIAGSSDYDQDLKALVEQNKSVNEIYEALALKDIAMAADLFRPLYDKTDGRDG
                     FISLEVSPTLANDTKGTIREARRYFLTLGRPNVMIKVPATKAGIPAITELIGAGVNVN
                     VTLMFSIEQYTAVAEA"
     gene            complement(600..2660)
                     /gene="tkt"
                     /locus_tag="VFJ67_00150"
     CDS             complement(600..2660)
                     /gene="tkt"
                     /locus_tag="VFJ67_00150"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7288818.1"
                     /translation="MESKPKISRDKLEQLIINTIRTLSMDAVQKANSGHPGTPMALAP
                     VAFAIWDKFMKFNPRNPDFPNRDRFVLSNGHASMLLYSLLHLTGYDVSLNDIKTFRQL
                     HSKCAGHPEYGLAPGVETTTGPLGQGVATSVGMAIAERWLAAYFNKPGHEILNYNVFA
                     ICGDGCMMEGISGEAASLAGHLGLSNLIWFYDNNHITIEGHTALAFSEDVAARFMGYN
                     WHVQRVGDANDLEMLAEAIDRALKENEQPSLVIVDSHIGYGSPNKQDTSAAHGEPLGD
                     EEIRKTKINYGWDPDKKFYIPEEVKEYRETVLRKGAVIEDEWNKKFNAYEHVYPELAK
                     QFRELEDREMPKGWEKCLPVFPPSPKGTATRSANSKILNAIVPVYPFLLGGAADVGSS
                     TKTYIDNAASFEKGIYDGRNFHFGIRENAMAASANGMALSKLRPYTATYFVFSDYMRA
                     PIRLACLMQIPVIFIFTHDSIGLGEDGPTHQPVEHLASLRAMPNLDVIRPADANELSV
                     LWKYILGLKNRPVALILTRQDVPVFDRGVYAPAEGALRGAYVLADCKSEPDIILIGTG
                     SEVQLCLGAYEILKKQVVKARVVSMPSWSLFEMQGAEYKEEVLPSSVTARVTVEAGST
                     FGWCRYAGSGDRSGMIGISTFGESAPIGDLLPEFGFTVDHVVAKANEVLNVKDKKAKK
                     SSKK"
BASE COUNT          511 a          805 c          802 g          716 t
ORIGIN      
        1 cgcctcggct acggccgtgt actgctcgat gctgaacatg agcgttacgt tgacgtttac
       61 gcccgcccct atcagctctg tgatggccgg gataccggct tttgtagccg ggacctttat
      121 cattacgttc gggcgcccga gcgtgaggaa gtacctcctc gcctcgcgga tggtgccctt
      181 cgtgtcgtta gcgagcgtgg ggcttacctc gaggctaata aacccgtcgc ggccgtccgt
      241 tttatcgtaa agcggcctga aaaggtcggc ggccatggcg atgtccttga gagcgagcgc
      301 ctcgtatatc tcgtttaccg atttattctg ctcgacgaga gctttgagat cctggtcgta
      361 atcggagctc ccggctatgg ctttttcaag gatggaaggg ttcgaggtct cccccctcaa
      421 gccctcttct attaaggcct ggagctctcc tgaagtaatg aaagaccgcc ttatgtagtc
      481 gtaccagatc gactggccga ccttggatag atcctttatt ctggacatcg tctctctctc
      541 ccgttcatcg agttatttgt tccacaaaac ggcgcacgac cgcaagactt aaatctacat
      601 tatttttttg aagatttttt agcttttttg tctttaacgt tgaggacctc attcgctttc
      661 gcgactacgt gatcgaccgt gaacccgaat tcgggaagca ggtctcctat cggggccgac
      721 tctccgaatg tgctgatgcc tatcatgccg ctccgatcac ccgagcccgc gtacctgcac
      781 catccgaacg tggagccggc ctcgacggtc acccttgccg taacggagga aggaaggact
      841 tcctccttgt attccgctcc ctgcatttcg aagagggacc aggagggcat gctgacgacg
      901 cgcgccttga ccacctgttt cttgagtatc tcatacgcgc ctaaacacag ctggacttcc
      961 gatcccgtgc ctatcaatat gatgtcgggt tcggacttgc agtctgcgag cacgtaagcc
     1021 cctctgagcg ccccctcggc cggggcgtat acgcccctgt cgaaaacggg gacgtcctgc
     1081 ctcgtcaaaa taagcgcgac cggacggttc ttgaggccca gtatgtattt ccacaggacg
     1141 ctcagctcgt tcgcgtcggc gggccttatg acgtcgaggt tcggcatcgc acggagcgac
     1201 gccaggtgct cgacgggctg atgcgtcggt ccgtcctcgc cgagtccgat gctgtcgtga
     1261 gtgaatatga atattaccgg tatctgcatg agacaggcga gcctgatggg agccctcatg
     1321 taatcggaga atacgaaata agtcgccgta tagggcctca gcttgctgag cgccatgccg
     1381 ttcgcggaag ccgccatcgc gttctctctt atcccgaaat ggaagttcct gccgtcgtat
     1441 atacctttct cgaagctggc ggcgttatcg atgtaggttt tcgtcgaaga gccgacgtcg
     1501 gccgccccgc ccaggaggaa cgggtacacg gggactatgg cgttcagtat cttgctgttg
     1561 gccgagcgtg tcgccgtgcc tttcgggctc ggcgggaata ccgggaggca tttctcccac
     1621 cccttcggca tctccctgtc ctcgagctca cggaactgct tcgcgagctc cgggtacacg
     1681 tgctcgtacg cgttgaattt tttgttccat tcgtcctcga tgacggctcc tttcctgagg
     1741 acggtctccc tgtattcctt tacctcttcc ggtatgtaga atttcttgtc cgggtcccac
     1801 ccgtaattga tcttggtctt gcgtatctcc tcgtcgccga gcggctcgcc gtgcgcggcg
     1861 gaagtgtctt gcttgttcgg gctgccgtag cctatatggc tgtcgacaat aacgagcgaa
     1921 ggctgctcgt tctccttgag cgccctgtcg atcgcctcgg ccagcatctc gaggtcgttc
     1981 gcgtctccca cgcgctgtac atgccagttg tatcccatga acctggccgc cacgtcctcg
     2041 ctgaaggcga gcgccgtgtg cccttcgata gttatgtggt tgttgtcgta aaaccagatg
     2101 aggttgctga gccccaggtg cccggctagg gatgccgcct cgcccgatat tccctccatc
     2161 atgcagccgt ccccgcagat ggcgaataca ttgtagttaa gaatctcgtg gccgggcttg
     2221 ttgaaatagg ccgcgagcca tctctcggca atcgccattc cgacggaggt ggcgacgccc
     2281 tggccgaggg ggccggttgt ggtctcaacg ccgggcgcga gcccgtattc ggggtgtccg
     2341 gcgcatttgc tgtggagctg cctgaacgtc tttatgtcgt tgagcgatac gtcgtaaccg
     2401 gtaaggtgga gtaggctgta aaggagcatc gacgcgtggc cgtttgacag aacgaacctg
     2461 tcgcggttcg ggaagtcggg gttcctgggg ttgaacttca tgaacttgtc ccagatcgcg
     2521 aacgccaccg gggcgagcgc catgggggtg ccgggatggc cggagttagc cttctggaca
     2581 gcgtccatgg acagagtcct gatcgtgttt atgatcagct gctcgagctt gtccctgctt
     2641 attttcggtt tggattccat tctgcttctc ctcgtttatt aattttgaga attattgtga
     2701 atttagtatt aagccggata aaaacgaaac cgacaacgca tttcgcgccg ttgtcgttgt
     2761 atcgggaatt acaccggcta ccgagtaaga aattgcgcct ttctcacaaa cgcggtgtaa
     2821 gcgttgaatt tgac
//
LOCUS       DASSJH010000008         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_543151, whole genome shotgun sequence.
ACCESSION   DASSJH010000008 DASSJH010000000
VERSION     DASSJH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543151"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2062
                     /gene="rpoB"
                     /locus_tag="VFJ67_00155"
     CDS             <1..2062
                     /gene="rpoB"
                     /locus_tag="VFJ67_00155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7288819.1"
                     /translation="RALMGSNMQRQAVPLIRTSSPIVGTGFESTVAKDSGVTVIAKRD
                     GIVEYVDSARVVVSSTGDTSDTEAGVDMYNLLKFQKSNQSTCWNQKPIVTKGQHIKSG
                     QVIADGPSTDFGELALGQNVLVAFMPWGGYNFEDAILMSERLAKEDRFTSIHIEEVEC
                     IARETKLGREEITRDIPNVSEDALRNLDESGIIRIGAEVKPGDIIVGKISPKGETQLS
                     PEDRLLRAIFGDKAGDVKDTSLRASPGIYGTVIDVKMLISRVVDKDERAKSIEDFEVT
                     RLKQDRDDEIKILKDDAFERIKSLVVGKTSSAKLTVSRKTVLKKGDKITEDILKTIPF
                     EKFADLTIEKGDEAEAEIKRIIERTLEQIDLTNVVYDQRISKLTKPDELPPGVLKVVK
                     VNIAVKRKLQVGDKMAGRHGNKGVISKILAEEDMPYLPDGAPVDMILNPLGVPSRMNV
                     GQVLETHLGWAAKELGDQINEVMEREFSPGALKTKLKEIYKSTGTEKLIDKMSDDDVL
                     AVARRLRYGIPVKSPVFDGASEEEIKNMLAATGLPEDGKTILFDGQTGEPFDQKVTVG
                     VMYMLKLHHLVEEKIHARAIGPYSLVTQQPLGGKAHFGGQRLGEMEVWALEAYGASYT
                     LQEFLTVKSDDVIGRTRIFDSIVKGDMNFEPGLPESFKVLQKELQALALDVDLLEEEE
                     QGGN"
     gene            2088..>2285
                     /locus_tag="VFJ67_00160"
     CDS             2088..>2285
                     /locus_tag="VFJ67_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288820.1"
                     /translation="MDVDTLFEKPKNPSEYSAVKISIASPDKIKEWSHGEVRKPETIN
                     YRTFKPERSGLFCAKIFGPVKD"
BASE COUNT          635 a          550 c          696 g          404 t
ORIGIN      
        1 cagggcgctc atgggatcga acatgcagcg tcaggcagtg ccgctgatac gcacgagctc
       61 tccgatagta ggcacgggct ttgaaagcac cgtggccaag gattcggggg tgaccgtgat
      121 cgccaagaga gacggtatcg tcgaatacgt cgattcggcg agagtggtag tcagctcgac
      181 cggagatact tccgacaccg aagcaggcgt cgatatgtac aacctgctta aattccagaa
      241 atcgaaccag agcacctgct ggaaccagaa gccgattgtc acgaaaggcc agcacataaa
      301 gtccggtcag gtcatagcgg acggcccttc cacggatttc ggcgagctcg cgctgggcca
      361 gaacgtgctc gtggcgttca tgccgtgggg cggatataac tttgaagacg ccatcctgat
      421 gagcgagagg ctcgcgaaag aagacaggtt cacctccatt cacatcgagg aggtcgagtg
      481 tatcgcgcgc gagacgaagc tcggaaggga agagataact agggacatac ctaacgtaag
      541 cgaagacgct ctcaggaacc tcgacgagag cggaataatc aggataggcg ccgaggttaa
      601 gccgggggac ataatagtcg gaaagatctc cccaaagggc gagacccagc tctctcccga
      661 ggaccgtctc ctgagggcca tattcggaga caaggcgggg gacgtgaagg acacgtctct
      721 cagggcctca cccggaatat acggcaccgt catagacgtg aagatgctca tctcgagggt
      781 cgtcgataag gacgagcgcg cgaagagcat cgaggatttc gaagttacgc gtctcaagca
      841 ggacagggac gatgaaataa agatattgaa ggacgacgcg ttcgagcgta tcaagtctct
      901 cgtcgtcggc aagacatcat ccgcgaagct caccgtatcg aggaagaccg ttctcaagaa
      961 aggggataag attacggaag atatattgaa aaccatcccc ttcgagaaat tcgccgactt
     1021 aaccatagaa aagggagacg aggccgaagc cgagatcaag aggataatcg agcggaccct
     1081 cgaacagata gatcttacga acgtagttta tgatcagagg atatccaagc ttacaaagcc
     1141 agacgagctg cccccgggcg tcctcaaggt ggtgaaggtc aatatcgccg taaagaggaa
     1201 gctccaggtc ggggataaaa tggccggaag gcacggcaac aaaggcgtaa tctcaaaaat
     1261 acttgccgag gaggatatgc cctatcttcc tgacggagcg cctgtcgata tgatactcaa
     1321 cccgctcggc gtgccgtcga ggatgaatgt ggggcaggta ctcgagactc acctcggatg
     1381 ggcggcaaag gagctcggcg atcagataaa cgaggtaatg gaaagggaat tcagtcctgg
     1441 cgcgcttaag acgaagctca aggagatata caagtccacg gggacggaga agctcataga
     1501 caagatgagc gacgatgacg tgcttgccgt cgccagaagg ctccgttacg gcataccggt
     1561 gaaatcgcct gtattcgacg gagcgtccga ggaagagata aagaacatgc tggccgcgac
     1621 aggactgcct gaagacggca agaccatact cttcgacggc cagacgggcg agccgttcga
     1681 ccaaaaagtt acagtgggcg ttatgtacat gctcaagctc caccatttag tcgaagagaa
     1741 gatacacgcg agggcgatcg ggccttactc gcttgtcacg cagcagccgc tcggaggaaa
     1801 ggcgcatttc ggcggacagc gtttggggga aatggaggtc tgggcgctcg aggcgtacgg
     1861 cgcatcgtac acgctgcagg agttcctgac ggtgaaatcg gacgacgtga taggaaggac
     1921 aaggattttc gattcgatcg tcaagggaga catgaatttc gagcccggcc tgccggagtc
     1981 gttcaaggtc ctgcaaaaag agctccaggc tcttgcgctg gacgttgacc ttctcgagga
     2041 agaagaacag ggcgggaatt gaaaaaaagt ttagggaggg agtgaacgtg gacgtagata
     2101 ccctatttga aaaaccaaag aatccgtcgg agtattcggc tgtaaagatt tccatagctt
     2161 ctcccgacaa gataaaagag tggtcccacg gcgaggtgag gaagcccgag accatcaact
     2221 acagaacttt caagcccgaa agaagcggcc ttttttgcgc gaagatattc ggtccggtga
     2281 aggat
//
LOCUS       DASSJH010000009         6349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_759903, whole genome shotgun sequence.
ACCESSION   DASSJH010000009 DASSJH010000000
VERSION     DASSJH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759903"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFJ67_00165"
     CDS             complement(<1..969)
                     /locus_tag="VFJ67_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7288821.1"
                     /translation="MIEKIIEYSARNKFLTITLVGFFAIWGYWAMKNTPLDALPDLSD
                     VQVIVFTDWEGRSPDLIEDQITYPIVTSMIAAPKVKYVRGQSMFGTSLVYVIFEDGTD
                     LYWARSRVLEYLNEVRGKLPEGVNPTLGPDATGVGWVFEYALVDESGKHDLAELRSFQ
                     DWYLRYWLESVPGVAEVASVGGFVKQYQVEIDPNKLVAYGIPINKVIEAIRMSNNDVG
                     GRVVEFASTEYYVRGRGYIKSVEDIENIPVSTNGNGTPVYIRDIANVELGPDIRRGVA
                     ELDGKGETVGGIVVMRHGENALNVIESVKEKIDDIKPSLPEGVKIVT"
     gene            complement(1031..2521)
                     /locus_tag="VFJ67_00170"
     CDS             complement(1031..2521)
                     /locus_tag="VFJ67_00170"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7288822.1"
                     /translation="MKGMKKVLKAGSLLIILALILLAAYRYGYKIPLLKEIPFFSQGH
                     EHQWRPVLAENGEIEYWTCAMHPSVRMKEPGKCPICGMDLIPVTNKPDPGKEPDAVQA
                     EKEEPAPAGDEGMSDMEGMEGMDHSKHGMGVPQAPEKQGEEKSVFTVSPERQQMIGVK
                     TEPVEMRSMDKTIRTVGRVTLDETKTEQIHTKFSGWIDKLYVDYTFEHVRKGEPLFSV
                     YSPELVSTQQEYLLALKSKEILSGSEFSDISGGAGSLLAATEKRLRLWDISESQIREL
                     AETGKVKDSLVIYSPVSGIVTEKNVFENMYVEPGTNLYTIADHSDVWVEVDIYENEIP
                     LVKEGTQAVMTLRSFPGEEFRGVVTFIPPHLDMKTRTVKARLEFPNPDMKLLPEMYAD
                     VNIDIPLGEKLAVPESAVISTGADNLVFVDKGGGNMEIRKVALGQRADGYYEVLRGLT
                     EGEKVVSRANFLIDSESRIQAAVATWGEDSPGEGSAGEHEGHDQGK"
     gene            complement(2573..2971)
                     /locus_tag="VFJ67_00175"
     CDS             complement(2573..2971)
                     /locus_tag="VFJ67_00175"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET7288823.1"
                     /translation="MRGRKLSIVTVLILVAVTALPYKTGYGTSMHEGISSFSQWHEQG
                     PQTILSAQGEIEYWTCPMHPDVKTGEPGKCPECGMDLIPVMKKPASGTQTAPDNSAEK
                     NGESPDTNTSKEETHEHSAHVKETPPPQKE"
     gene            complement(2968..4251)
                     /locus_tag="VFJ67_00180"
     CDS             complement(2968..4251)
                     /locus_tag="VFJ67_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7288824.1"
                     /translation="MKAALGALLLILFLTPGIYAEEAVTLDSLVQEALQNNPEIKAAK
                     ARWQASSKRPSQEGTLPDPMIGVDWQNVTFDGITLDEDPNSMLRLEFEQEIPFPGKLS
                     SKKKIALRDAEAEEQSYLATERRVIADLKAAYYDWYLAGKAIEITERSKEVLQKFTKI
                     AEVKYEVGKGIQQDVLKAQVENSKFIEQLEVLKERKELVEAKIRSILNREHDNPLGSP
                     VDIEITPLTLTSEEAGELTRENAPLLAMREREIAKGEEALVLAKKEIYPDFVIGASPG
                     IMGREGNGVDGIWEVSLGLKVPLYFWSKQKPGIEQAALELKGAQEEYSSVGQELNYSV
                     RESYLRARTAEKLMSLYESGIIPQSKLSLESAISGYQVGSVDFLTLLDNLVAVFSFEL
                     EYYNQLAEYEKAVARIEELSGIQIANQTNQKEEQK"
     gene            complement(4369..4764)
                     /locus_tag="VFJ67_00185"
     CDS             complement(4369..4764)
                     /locus_tag="VFJ67_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288825.1"
                     /translation="MAMMRFASLFLALNILLCYAGVYRNALADSGLPRATTSGGCHGM
                     SRESNASESNAVTGIVNDSESRGASCCQDTLENAHPDLNTRIQVVTIDRSHLHVINES
                     KTNLHKFLDGSPREHDPPDLQVSFSTFLL"
     gene            4944..5939
                     /locus_tag="VFJ67_00190"
     CDS             4944..5939
                     /locus_tag="VFJ67_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7288826.1"
                     /translation="MEYIKIGGTDITSSRIGLGCWAIGGWLWGGSEERESVDTILAAL
                     GRGINLIDTAAIYGFGRSEEIVGKALSEYGRRDKVVIATKVGLDWTSGKVYRNSTRDR
                     IMQEVEDSLKRLRTDYIDIYQIHWPDSDTPIEETASAMDELYRSGKIRAIGVSNYSTQ
                     QMDDFRRKAPLHISQPPYNLFERGIEVDILPYCQSNGITMFFYGAICRGLLSGRMRAD
                     TKFEGDDIRKADPKFIEPRFGQYLEAVEKLEEYARDKYKKRVIHLAVRWILDKAPNHI
                     ALWGARRPAQLDPIAEMMGWSLDTDDFRSIDKILSETIKDPVGPEFMAPPHRPKA"
     gene            complement(5949..>6349)
                     /locus_tag="VFJ67_00195"
     CDS             complement(5949..>6349)
                     /locus_tag="VFJ67_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7288827.1"
                     /translation="VSSLSPLLETLRQRRYMLAVASSSPLRVINFVLDMFSLHAYFDA
                     VVSGECTENGKPQPDIYLHTAERLGVDPSECVAIEDSINGVRSAIGAGMYCIAVPDKR
                     LSREPFESAHMILESLDQLRPEVFESLRTL"
BASE COUNT         1255 a         1845 c         1626 g         1623 t
ORIGIN      
        1 ggtcacgatc ttcactcctt cgggcagcga cggttttatg tcatcgatct tctctttcac
       61 actttctatc acgttgagag cattttcacc gtggcgcatg acgacgattc cgccgaccgt
      121 ctctcctttc ccgtcgagct cggcaacgcc tctccttata tcgggaccga gctccacatt
      181 ggcgatgtcc cgtatataga cgggggtccc gttcccgttc gtcgagacag gtatattctc
      241 aatatcctct actgacttta tatatcctct ccccctcacg taatactcgg ttgacgcgaa
      301 ttcgacgacc ctgcccccga cgtcgttgtt gctcatccgg atcgcttcta tcaccttgtt
      361 tatcggtatg ccgtaggcta ctagcttgtt ggggtcgatc tccacctggt actgctttac
      421 gaagccgcca acgcttgcca cctccgcgac gccggggacg gactcgagcc agtatctcag
      481 gtaccagtcc tgaaaagagc ggagctccgc caggtcgtgc ttgccggatt catccactag
      541 cgcgtattca aaaacccatc ctaccccggt ggcgtccggc cccagcgtcg ggttgactcc
      601 ttcggggagt tttcccctga cctcgtttaa atattcgagc acgcggctac gcgcccaata
      661 gaggtccgtg ccgtcctcga agatcacgta gacgagagat gtgccgaaca tagactggcc
      721 tctcacgtat ttcactttgg gagcggctat catgctcgtg acgatagggt aggtgatctg
      781 gtcttctatc aggtccgggc ttctcccctc ccagtcggtg aacacgatga cctggacgtc
      841 ggacagatcg ggcagcgcgt caagcggagt gttcttcatc gcccagtatc cccagatagc
      901 gaagaagcct acgagcgtta tcgttagaaa cttgtttctg gccgagtatt ctattatttt
      961 ttctatcatc tacatttcct ctaaagtcgc atgaaccgta cgctgcgccg ctccccgtcg
     1021 tccgccctga tcatttgccc tgatcgtgtc cttcgtgctc tcccgcgctc ccttcgccgg
     1081 gactgtcctc gccccacgtc gcgaccgcgg cctgtatcct gctttccgaa tctatgagaa
     1141 aattggcccg tgagacgacc ttttctccct ctgtcagccc cctgagcacc tcgtagtatc
     1201 cgtccgctct ctgcccgagc gccacctttc ttatctccat attcccgccg cccttgtcga
     1261 cgaaaacgag attgtccgcc cccgtgctga tgaccgccga ttcgggcact gccagtttct
     1321 cgccgagggg gatatctata ttgacatcgg cgtacatttc agggagcagc ttcatgtcgg
     1381 gattggggaa ttctagacgc gccttcaccg tcctcgtctt catgtcgaga tgcggcggta
     1441 tgaacgtcac aacgcccctg aactcctctc ccggaaacga cctgagcgtc atcacggcct
     1501 gagtaccctc cttcacgagg ggtatctcgt tctcgtatat gtccacctcc acccaaacgt
     1561 ccgaatggtc ggcgatcgta tagagattgg ttccgggctc gacgtacatg ttctcgaata
     1621 cgttcttttc cgttacgatt cccgatacgg gcgagtagat gacgaggcta tccttcacct
     1681 tccctgtttc cgcgagctcc cgtatctggc tctcggagat gtcccagagc ctgagacgct
     1741 tctcagtagc tgcgaggagc gagcctgcgc cgcccgatat atcggagaat tcgcttcccg
     1801 acagtatctc cttcgacttg agagcgagga gatactcctg ctgggtggat acgagctccg
     1861 ggctgtatac ggagaacagc ggctcgcctt tcctaacgtg ctcgaacgtg taatcgacgt
     1921 agagcttgtc gatccatccg ctgaatttcg tgtggatttg ctccgtcttc gtctcatcga
     1981 gcgtcaccct cccgaccgta cgtatcgttt tatccatcga gcgcatctcg acgggctcgg
     2041 ttttgacccc gatcatctgc tgcctctccg ggctcacggt gaaaacggat ttctcctcac
     2101 cctgtttttc cggggcttgc gggactccca taccgtgctt ggaatggtcc atgccctcca
     2161 taccttccat gtcggacatc ccttcgtcgc cggccggtgc gggttcttct ttttcagctt
     2221 gtacggcatc cggctctttc cccggatcag gcttgttcgt tacgggtatg agatccattc
     2281 cgcatatcgg gcatttcccc ggctccttca tcctcacgga aggatgcatc gcgcacgtcc
     2341 aatactcgat ctccccgttc tccgccagca cggggcgcca ctgatgctca tgtccctgcg
     2401 agaagaacgg tatttcctta agcaaaggaa tcttgtaacc gtatctgtat gcggctaaga
     2461 gaataagcgc caggattatt aataaagagc ccgctttcaa aacttttttc atacctttca
     2521 ttgtttttgc tccttaccga aagtttgttt ccgaaactcc gttgcatcat gcctactcct
     2581 tttgcggagg cggcgtctct ttaacgtgag cgctatgctc atgtgtttct tcttttgaag
     2641 tatttgtatc cggtgattct ccgttcttct ctgcgctgtt atccggagcg gtttgagtcc
     2701 cagaggccgg ttttttcata acggggatca ggtccatgcc gcattcgggg catttacccg
     2761 gttctcccgt cttgacgtcc gggtgcatgg ggcatgtcca atactcgatc tctccctgcg
     2821 cggaaagtat tgtctgcggc ccttgctcgt gccattgtga aaaggacgat attccctcat
     2881 gcatagaagt cccgtagccg gttttgtagg gtagtgctgt tactgccaca aggattaaaa
     2941 cagtcactat cgataatttt cttcctctca tttttgttct tccttttgat tggtttgatt
     3001 tgcaatttga attcctgata actcctcgat cctcgccacg gccttctcat attcggcgag
     3061 ctgattgtag tattcgagct cgaagctgaa cacggctacg aggttgtcga gaagcgtgag
     3121 gaaatcgacc gaccccacct gataacccga aatcgcggat tcgagcgaca gcttcgactg
     3181 cgggattatc ccgctctcgt agaggctcat gagcttctcg gccgtcctcg ctctcaggta
     3241 actctccctc acgctgtaat tgagctcctg gcccaccgag ctgtactctt cctgcgcgcc
     3301 tttcaattcg agggccgcct gctctatgcc cggcttctgc ttgctccaga aatagagcgg
     3361 cacttttaat ccgagcgaca cctcccagat tccgtcgacc ccgttccctt cccgtcccat
     3421 tatcccgggg gacgccccga tcacgaagtc gggatagatc tccttctttg cgagaacgag
     3481 cgcttcctct cccttcgcta tctccctctc tctcatggcg aggaggggcg cgttctccct
     3541 cgtgagctcg cccgcctcct ccgacgtcag cgtgagaggg gttatctcta tgtccacggg
     3601 gctgccgagc ggattgtcgt gctcgcggtt gagtatgctt cttatcttcg cctcgacgag
     3661 ctcctttctt tccttgagca cctcgagctg ctctatgaac ttcgagttct ccacctgggc
     3721 tttgagcacg tcctgctgta taccctttcc cacctcgtac ttcacttccg cgattttggt
     3781 gaacttttgg agaacttcct tactcctttc ggtgatctct atggcctttc cggcaaggta
     3841 ccagtcgtag taggcggcct tgagatcggc gatcactctc ctctccgtag cgaggtagga
     3901 ttgctcttcc gcttcggcgt ctctcaaggc gatcttcttt ttcgaggaca gctttccggg
     3961 gaacgggatc tcctgctcga actcgagcct cagcatgctg ttcgggtctt cgtcgagcgt
     4021 tataccgtcg aaggtcacgt tctgccaatc gacccctatc atcgggtcgg gaagcgtccc
     4081 ttcctgagat ggacgcttgg acgacgcctg ccacctggct ttcgccgctt tgatctcggg
     4141 gttattctga agcgcctcct ggacgagtga atccagtgtc acggcttctt cggcataaat
     4201 gccgggcgtc agaaataaaa tcagtaacaa tgctcccagt gccgctttca tatatgtgcc
     4261 tcctcttaag cgaggaattc caaagattta ggattatgga aatagagaaa tccccggccg
     4321 ccctgttata cggtcaggcg gttacgggaa ctgaaggaaa ggtcggaatt atagtaaaaa
     4381 ggtggaaaaa gaaacctgta aatcgggagg atcgtgctcc ctcggggagc cgtccaggaa
     4441 tttgtggaga ttggttttac tttcgttgat tacatgcagg tgtgatctgt ctatggttac
     4501 tacctgaatc ctggtgttta gatccggatg tgcgttctcc agcgtatcct ggcagcatga
     4561 cgcgccccgg ctttccgaat cgttaacgat tcccgtaacg gcattagact cggaagcatt
     4621 gctttcccgg ctcatgccgt ggcacccgcc gctagtcgtt gcccggggta ggccgctatc
     4681 tgcgagtgca ttcctgtaca ctccggcgta gcagaggaga atattcagcg ctagaaacag
     4741 gctcgcgaat cgcatcatcg ccatttatca tagcatatag cttaataccc cgatgtcaaa
     4801 tatatttgag tccggctacg gttgccggcc ggtccgtgcg tcgaatagta ggggcgccgg
     4861 cgggcgggta atatataacg ctgagataca ccggttggct gcattttccg ttaaaaatca
     4921 atgttccccg ggggtactgt cacatggaat acattaaaat cggcggaacc gatatcacct
     4981 catcgcgtat cggtctcggc tgctgggcta taggagggtg gctctggggc gggagcgaag
     5041 agcgcgaatc tgtggacact atactcgccg cgctcggcag gggtattaat ctaatcgaca
     5101 cggccgcaat ctacggcttc ggaaggtcgg aggagatcgt cggtaaggca ctctccgagt
     5161 acggccggag ggacaaggtc gtcatcgcca ccaaggtcgg tctcgactgg acttcgggca
     5221 aggtctacag gaattcgacg agggaccgca ttatgcaaga ggtcgaagac tctctcaaga
     5281 ggctgcggac ggattacata gacatatacc agatccactg gcccgactct gataccccga
     5341 tcgaggagac tgcatcggca atggacgagc tctacaggtc aggcaaaatc agggcgatag
     5401 gcgtgagcaa ctattcgacc cagcagatgg acgacttcag gaggaaagcc ccgctccata
     5461 tctcacagcc cccctataac ctcttcgaga ggggcatcga agtcgatatc cttccctatt
     5521 gtcaatcgaa cggcatcacg atgttttttt acggtgcaat ctgccggggc ttactcagcg
     5581 gcaggatgag agccgatacg aaattcgagg gcgacgacat caggaaagcc gaccccaagt
     5641 tcatagagcc caggttcgga cagtatctgg aagcggtgga aaaacttgag gagtacgcgc
     5701 gagataaata taagaaaaga gtcattcatc tggccgtgcg atggattctc gataaggcgc
     5761 ccaatcacat agctctatgg ggggcgagac ggccggccca gctcgatccg atcgctgaaa
     5821 tgatgggatg gtccctcgat acggacgact tccgctccat agacaagatt ctctcggaaa
     5881 ccattaaaga ccctgtgggt cccgaattca tggcgccccc gcataggccg aaggcctgaa
     5941 ttgtattttt ataaagtacg gagggactcg aacacttccg gtctcagctg gtccaggctt
     6001 tcgagtatca tgtgagcgct ttcgaacggt tccctgctga gcctcttgtc agggacggct
     6061 atgcagtaca ttccggcccc tatggcggac ctgactccgt tgatagaatc ctcgattgcg
     6121 acacactcgg acggatccac cccgagcctt tccgctgtgt ggagatatat atccggctgc
     6181 ggttttccgt tctccgtgca ttccccggaa accacggcgt cgaagtaagc gtggagcgag
     6241 aacatatcga gcacgaagtt gatcaccctg agcggcgaac tcgaagccac agccagcatg
     6301 tatcttctct gcctgagggt ttccagaagc ggtgaaagcg acgatacga
//
LOCUS       DASSJH010000010         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_218964, whole genome shotgun sequence.
ACCESSION   DASSJH010000010 DASSJH010000000
VERSION     DASSJH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218964"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VFJ67_00200"
     CDS             <1..993
                     /locus_tag="VFJ67_00200"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7288828.1"
                     /translation="IFLKRIDDGFIKDCHGDLHLDHINIRPEGICIYDCIEFNERLRY
                     IDTASDIAFLAMDLDYNGCPYLARFFVAETAGAMGDPGMYRLLDFYKCYRAFVRGKVE
                     SIMAFEPEVPGDEKALSLEKAKKYFRFALRYALFGSAPAVIVTFGIIGTGKSTLARML
                     GDELSCPIVSSDAVRKEITGTPAGERKYEGWEGGIYTSDITERTYGEIIGRALAEAAN
                     SLTVILDASFSKRKWRETLMREAGGSGVSVYFVRTTAPVEEVELRLLRREKEGTSISD
                     ARAAILPRFLAEWEEPVAAEGALIFRADTSKPGHEVIDSLLKDVIDAGFSRSKQ"
     gene            1058..>2589
                     /locus_tag="VFJ67_00205"
     CDS             1058..>2589
                     /locus_tag="VFJ67_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7288829.1"
                     /translation="MITTGEPWKISEEDVLTGLGVSPEEGLGNSEALERLEKYGLNRL
                     VEAKPASALSILIAQFRSLIVLLLVAAALLSFLFDDLPEGAAIVAVIILNAAIGFFTE
                     IRAVRSMEALYRLVRVEATVLREGSVSVIRAEELVPGDIVVLEAGDVVPADVRLIEAS
                     RVEANESSLTGESAPVRKFTDTLTGEAPLAERFNMLFMGTALTKGSARGVVTATGAFS
                     ELGKISVLAKEAGDDRTPLEKKLDRLGNRLIWVTLVVAAAVIAGGAITGRDFLLMVKT
                     GIALAVAAIPEGLPVVATIALARGMLRMARRNALVNRLASVETLGATNVICTDKTGTL
                     TENMMTVEEIATEGGDLRFDWKNGKFLINGRESDAVTDRAAVAAIETGVLCNRASLGA
                     GPPGEGGDIGDPLEIALLRAGVLAGIEREPLLSVFPETGVEAFDTLVNMMATFHESDG
                     AFRVAVKGAPDAVLGCCDSILTPSGAAGLTEDRREFWRLRNEELASTGYRVIAHAGKT
                     VSV"
BASE COUNT          561 a          706 c          864 g          458 t
ORIGIN      
        1 atattcctga aacgcatcga tgacggcttc attaaggact gtcacgggga cctccacctc
       61 gaccacatca atatccggcc ggaaggcata tgcatctacg actgcataga gttcaacgag
      121 aggttgagat acatagacac ggcctcggac atagccttcc tcgcgatgga cctcgactac
      181 aacgggtgcc cgtatctcgc gcgttttttc gtcgcggaga ctgcgggggc catgggcgac
      241 cccggaatgt accggctgct ggatttttat aaatgctaca gggctttcgt cagaggtaag
      301 gtcgagagca taatggcgtt cgagccggag gttcccgggg acgagaaggc cctgtccctg
      361 gagaaggcga aaaaatactt caggttcgcg ctcagatacg ccctcttcgg ctcggctccc
      421 gctgtcatag tcacgttcgg aataatcggc acgggaaaat ccacgctcgc ccggatgctc
      481 ggcgacgagc tctcctgccc gatagtttca tcggacgcgg tgaggaagga gataaccgga
      541 acgcccgccg gggagaggaa atacgagggc tgggagggcg gaatatatac gagcgatata
      601 acggaaagga cttacggaga gatcataggc cgcgcgctcg cagaggccgc gaatagcctg
      661 accgtaatac tcgacgcgag cttctcgaag agaaaatgga gagagacgct gatgcgcgag
      721 gccgggggct cgggggtctc cgtttacttc gtcaggacga cagcgcccgt ggaggaggtg
      781 gagctcaggc tgctcaggag ggagaaggag gggacttcga tctcggacgc gagggccgcc
      841 atactgccga ggttcctggc cgagtgggag gagcctgtgg cggcggaggg cgcgcttata
      901 ttcagggccg atacctcgaa gcccgggcac gaagtgattg attcgctgtt aaaagatgta
      961 atagacgccg gattttcccg gtcgaagcag taacctcacc gtctgcggcc gcgtgacggt
     1021 cacggaccgt accgcaatat tttcattacg atagatcatg atcacgaccg gagaaccctg
     1081 gaagatttcc gaagaggacg tgcttaccgg cctcggggtc tctcccgaag aagggctcgg
     1141 taacagcgag gcgctcgaga ggctcgaaaa atacggtctc aaccggctcg tagaggccaa
     1201 acccgcaagc gcactctcca ttctcatcgc ccagttcagg agcctcatag tgctcctcct
     1261 cgtagcagcc gccctgctct cttttctctt cgacgacctg ccggaagggg ccgcgatagt
     1321 ggccgtgata atccttaacg ccgcgatcgg gtttttcacc gagatacgcg ccgtgaggtc
     1381 tatggaagct ctctacaggc tcgtccgcgt ggaggcgacc gtgctccggg aggggagcgt
     1441 atcggtcata cgcgccgagg agctagtacc cggcgatata gtcgtgctcg aggcgggaga
     1501 cgtagtgccc gcggacgtga ggctaatcga ggcctcgagg gtggaagcga acgagtcctc
     1561 gctcacgggg gaatccgctc ccgtgaggaa gtttacggac acactcacgg gagaagcgcc
     1621 cctcgcagag cgttttaaca tgctgtttat gggcacggcg ctcacgaaag gctcggcaag
     1681 gggagtcgtc accgcgaccg gcgcgttctc ggagctggga aagattagcg tcctcgcaaa
     1741 ggaggcgggg gacgacagga cgcctctcga gaagaagctc gacaggctcg ggaacaggct
     1801 gatatgggta actctcgtcg ttgccgcggc cgtgatagcc gggggcgcga taacggggag
     1861 ggacttcctt ctcatggtga aaacggggat agcgctagcc gtggccgcca tacccgaagg
     1921 gctccccgtc gtggctacga tagcgctcgc tagggggatg ctgaggatgg cgcgaaggaa
     1981 cgcgctcgtc aaccgcctgg cctcggtcga gaccctgggc gcgacaaacg tcatatgcac
     2041 ggacaagaca gggacgctga ccgaaaacat gatgacggtc gaggagatcg ctaccgaagg
     2101 cggggatctg aggttcgact ggaagaacgg gaaatttctc ataaacgggc gtgaatcgga
     2161 tgcagtcacg gacagggcgg cggtcgcggc catagagacg ggcgttttat gcaacagggc
     2221 gagcctcggc gcggggcctc ccggggaagg aggagacata ggcgacccac tcgagatcgc
     2281 tctcctgagg gcgggggtac ttgccgggat agagagagag ccgcttctgt ccgttttccc
     2341 cgaaacgggt gtcgaggcgt tcgatacgct cgtgaacatg atggccacgt tccacgaatc
     2401 ggacggggct ttcagagtgg ccgtgaaagg cgcccccgac gcggtgctcg gatgctgcga
     2461 cagcatcctt acgccctccg gcgcagccgg gctcactgaa gacaggaggg aattctggcg
     2521 tctgaggaac gaggagctcg cgtccacggg atacagggtt atagcccacg ccgggaagac
     2581 cgtctccgt
//
LOCUS       DASSJH010000011         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_436103, whole genome shotgun sequence.
ACCESSION   DASSJH010000011 DASSJH010000000
VERSION     DASSJH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436103"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2039
                     /locus_tag="VFJ67_00210"
     CDS             <1..2039
                     /locus_tag="VFJ67_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7288830.1"
                     /translation="EGGGSGVRNLYYLSKPKILGRDIKRFHDIVGFKKMAILYMPLLK
                     ELMPGIENTLRKISADLDVVITLVPARASVGETLAAIPPDTQAVYVTPLLDYSDAERI
                     ELYDGLLERNLPSFSMLGKGEVETGALVGLAPDHDFTREARRVALVVQSILLGNEAGD
                     YPVEFSEEETLTINMATARAIKFSPSWSVLTDAVLINEEPAQKGRTLTLEQAVNESVE
                     VNLDLLAANQFVAAGAQDIKLAMAQLLPQSDIFLDNTYIDKDRAEASLGTQPQRDLSA
                     GGNASQLIYSETAWSNLTVQRRLQESREFTRDEVRLDIIAATAIGYLNVLRAKTIERI
                     QKENLGLSKSNLELAKVRRDIGVASPAEVFRWESQISSDRINVVNSEADRKNTEILVN
                     RLLHRPQGENFTTVESGLEDPSLLVSDPRLKRYISNPADFKTFMDFMVSLGLNQSPAI
                     SAIDSQMAAQDRILTSTKRAFWSPTIFAFGELRHFLYEGGAGSNVNIPSDFPIDITNT
                     DDTEWTVQLQASFPLFRGGAKIADYRKARSELSRLRYERDSTAEKVEEQIRTSLNASG
                     ASYPSIGFALDAAEAAHKNLELVTDEYSRGVVSIIDLLDAQNAALSADLNAANSVYNF
                     LIDLMNVQRAAGKFDFFTSPAEREQWFRMLEEFYNKTTEADETNSADAEGGATN"
     gene            2083..3159
                     /locus_tag="VFJ67_00215"
     CDS             2083..3159
                     /locus_tag="VFJ67_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7288831.1"
                     /translation="MRRTTNGLVLRTAAALALLIILGSCGGEEKDETQVQVIRPVKTI
                     ILGGGQGVGRSFPGKVQGSQRVNLSFRVRGPLIEFPVNEGDEVKQGQLLARLDPRDYQ
                     IAYDEAYAQFVEARADYKRYKDLYERNAVPIADLDVRRAKLDTAKAKLDYAKANIDYT
                     YLKAPFSGYVGAKFVENYQEVNSFDQILSLQDLSKVEIVIDLPENILASTKEGDVAQL
                     YATFQSAPGEEFPLIIKEVSAQADPRTQTYRATLSMDQPGTIRVLPGMTAQVHLKGKT
                     NNISNGNYFIIPAIAVVTGDGPDQYVWVVDQNDMTVHKRVVQVGAVTGQKDIEILGGL
                     KAGEMIAVTGITQLKEGMQVTLLN"
BASE COUNT          851 a          823 c          921 g          594 t
ORIGIN      
        1 ctgagggtgg agggagcggc gttaggaacc tctactatct gtccaagcct aagatactcg
       61 gcagggacat aaagaggttc catgatatag tcggctttaa aaagatggcc atcctgtata
      121 tgccgctctt gaaggagctt atgcccggga tcgagaatac gttgagaaaa atttcagctg
      181 acctggacgt ggtcataacc ttagtccccg cgagagcttc cgtcggagaa acgctcgccg
      241 caatacctcc tgacacacag gcggtttacg ttacccctct tctcgactat tccgacgccg
      301 agaggatcga gctctacgac ggtcttttag aaaggaacct cccctcgttc tcaatgctag
      361 ggaagggaga agttgagaca ggggcgctcg tgggccttgc tccggatcac gactttacga
      421 gagaggcgag gcgggtggcg ctcgtcgtac agagcattct tctcggaaac gaggcagggg
      481 attatcccgt ggaattctca gaagaagaaa cgctcacgat caatatggcg accgcccgcg
      541 cgatcaagtt ctccccgtcg tggagcgtcc tgacggatgc cgtcctcata aacgaagagc
      601 cggctcagaa aggcaggacg ctcaccctcg aacaggcagt gaacgaatcc gttgaagtga
      661 acctcgacct cctggccgcg aaccagttcg tagccgcggg agcccaggac ataaagctcg
      721 ctatggcgca gcttctcccg cagtcggaca tattcctcga caacacatac atagacaagg
      781 acagggcgga agcgagcctc ggaacacagc cccagcgaga cctgagcgcg ggaggcaacg
      841 cgagccagct catatattcg gaaaccgcgt ggtcgaacct gaccgtacag cgtcggctcc
      901 aggaatcgag agagttcacg cgggacgaag tgaggctcga catcatagcg gcgacggcga
      961 taggatatct gaacgtgctc cgcgcgaaga cgatagaaag gatccagaag gaaaacctcg
     1021 gcctatcaaa atcgaacctt gaactggcga aggtaaggcg agatatcggg gtggcaagcc
     1081 ctgctgaggt tttcaggtgg gagagccaga tatcctcgga cagaataaac gtggtaaact
     1141 ccgaagccga caggaagaat acggagatac tcgtcaacag gctccttcac aggccgcagg
     1201 gcgagaattt tacgaccgtt gagtccgggc tcgaagaccc ttcgctcctc gtgagcgacc
     1261 cgaggctcaa gcgctacata agcaaccccg ccgatttcaa gacattcatg gatttcatgg
     1321 taagccttgg gcttaatcaa tctcccgcaa tatcggcgat cgattcccag atggccgcgc
     1381 aggacaggat cctcacctcg accaaaagag ctttctggtc gccgactata ttcgccttcg
     1441 gagagctgag acacttcctt tacgaaggtg gagccgggag caatgtaaat atcccgagtg
     1501 atttcccgat agatataaca aatacggacg atacagaatg gaccgtccag ctccaggcgt
     1561 cgttcccgct tttcagagga ggagcaaaaa tagccgacta caggaaggcg aggagcgagc
     1621 tgtcgagact caggtacgaa agggactcca ccgcagaaaa ggtcgaggag cagataagga
     1681 cctctctcaa cgcgtcaggg gcgtcttacc cgagcatagg gttcgcgctt gacgccgcgg
     1741 aggccgcgca taagaacctc gagctcgtca cagatgagta ctcccgcggg gtggtatcca
     1801 tcatagacct tctcgacgct cagaacgccg ccctctcggc cgatctgaac gccgcgaact
     1861 ccgtctataa tttcctcata gacctgatga acgtgcagcg ggcggcaggg aaattcgatt
     1921 tctttacgtc tcccgccgag cgggagcagt ggttcaggat gctcgaggaa ttctacaaca
     1981 agacgacgga agcggacgaa acaaatagcg ccgatgctga aggcggcgcg acgaattaga
     2041 ctacgcagat tgatgcatga tccattgtca gagggacaaa gaatgagaag aacaacgaac
     2101 ggtttggttt tgagaacggc agcggccctg gccctgttga ttatactcgg ctcctgcggt
     2161 ggcgaggaga aagacgaaac ccaggtccag gtcataagac cggtgaagac gattatattg
     2221 ggcggcgggc agggtgtcgg aaggagtttc cctggcaagg tacagggatc acagagggta
     2281 aacctctcgt tcagggtgcg gggaccgtta atcgagttcc ccgtaaacga gggcgacgag
     2341 gttaagcagg gccagcttct cgcaaggctc gacccgaggg actatcagat agcttacgac
     2401 gaggcttatg ctcagttcgt cgaggcccgg gcggattaca aacgatataa agacctatat
     2461 gagaggaacg ccgtgcctat agccgacctt gacgtcagaa gggcgaagct ggacacggct
     2521 aaggcgaagc tcgactatgc gaaagcaaat atcgactata cctatttgaa agcaccattc
     2581 agcggatacg tcggcgcgaa attcgtcgag aactatcagg aagtaaattc cttcgaccag
     2641 atactgagtt tgcaggacct ctcaaaagtt gagatcgtga tcgatctccc ggagaatata
     2701 ctggcttcta cgaaagaggg ggatgttgcg cagctctatg ccacattcca gtccgctccg
     2761 ggcgaggaat tccccttaat tataaaagaa gtaagcgcgc aggccgaccc gagaacccag
     2821 acgtacaggg cgactctcag catggatcag cccggaacga taagggtgct tccgggaatg
     2881 acggcgcagg tacacctgaa gggaaaaaca aacaacatca gtaacggaaa ctacttcatc
     2941 atcccggcga tagctgtcgt cacaggggac ggccctgatc aatacgtttg ggtagtggat
     3001 caaaacgata tgacggttca caaaagggtg gttcaagtgg gggcggtaac cggacagaag
     3061 gacatcgaga ttctgggcgg actcaaagca ggcgagatga tagccgtaac cggcatcacg
     3121 cagctcaagg aaggtatgca ggttacgctc ctcaactaga tgaggtttct ccccaatgaa
     3181 aatagctga
//
LOCUS       DASSJH010000012         3946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_652465, whole genome shotgun sequence.
ACCESSION   DASSJH010000012 DASSJH010000000
VERSION     DASSJH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652465"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..788
                     /locus_tag="VFJ67_00220"
     CDS             27..788
                     /locus_tag="VFJ67_00220"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET7288832.1"
                     /translation="MRKDDGAIAELTINRPESYNSLSIGCMEALTSEFESLARDNNVS
                     VVILSGAGKGFCAGHDLKEMRSNPGREFYEKTFDTCSRLMMSIVGCPKPVIAMVHGVA
                     TAAGCQLVATCDLAVADENARFATPGVNIGLFCSTPMVALSRNVSRKHAMEMLLTGDF
                     ITAPRAYEIGLVNRVVPSGDLDLITHELAGKIASKSPLTLRIGKKAFYEQADRDLRGA
                     YEYCSRVMVENMMARDAEEGIDAFLEKREPHWKGV"
     gene            978..2057
                     /locus_tag="VFJ67_00225"
     CDS             978..2057
                     /locus_tag="VFJ67_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288833.1"
                     /translation="MRLTYGLIIAVLLYFAQAAASQAQSNSVSLPVIMGSKQVLVIGE
                     SYGHTESAEFFSKTVTEYLNNGGCIKVGLEISSDQQATLDSAMKGEVPVSELKINDIV
                     DSPAYRRMLTDLSGQVRAGKCLSVSAIDAPASVPVTRDAWMEKQVTALTGDTPVILLV
                     GNVRAVKDTGAGDSGGLLAERMSGKMPAVASVLQYWTPGQCENRTVEYINAEDERAGV
                     YLKETVKDMTPVMPATPTAIADGVLVWSCESENVTEKMNIDDSGKGESVKDQVGVTME
                     KTETVVRDEKALKKIKWGMKNEYPAIGMTKDEALQAMGEPENKEDVNGVERWSYQCFD
                     EDGYWHTCFTLDFKDDIVVKFLDLE"
     gene            complement(2244..2579)
                     /gene="rplS"
                     /locus_tag="VFJ67_00230"
                     /pseudo
     CDS             complement(2244..2579)
                     /gene="rplS"
                     /locus_tag="VFJ67_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(2595..3896)
                     /gene="trmD"
                     /locus_tag="VFJ67_00235"
     CDS             complement(2595..3896)
                     /gene="trmD"
                     /locus_tag="VFJ67_00235"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7288834.1"
                     /translation="MRFDILTIFPEFFESPFSLGIFKKASEKGIVELKTHDIRDYTED
                     RHRTVDDSPYGGGGGMLMKPGPIGKAVENAKRKGPNPLVILTTPAGEIFTDKTARELS
                     GYDQLIIICGRYEGIDDRVREIYVDREISIGDYVLSGGEYAAAVIVDSVARFIPGFLG
                     NVSSPESDSFKDWLLEYPQYTRPEEYMGRKIPEVLLSGNHKEIDEWRRRESVKRTYLR
                     RPELLDRARLTLDDITAANDLKKTSRPDFKAYIALVHYPVYNNRLTVITTAFTNLDVH
                     DISRAGKTYGIEKFYLVQPNPEQQKLAERVLRHWTEGEGPALNKSRAEAMEFVEISDS
                     LGDAVSDIEKIEGRKPVIVVTDARPRNNMIGYGELRDLMAGEDSCPFLLVFGTGWGLS
                     EEIMQGADYTLKPVSGYTGYNHLSVRSAAAIILDRLFSCTI"
BASE COUNT          868 a         1055 c         1067 g          956 t
ORIGIN      
        1 ccgaacaaaa ttcagcacaa ctcgtcttgc ggaaggacga tggagcgatt gccgagctta
       61 cgataaacag gccagagtcc tataactctc tgtctatagg ctgcatggaa gctctgacct
      121 ctgagttcga gtcgttagcg cgggacaaca atgtcagcgt cgtaatactg tcgggcgcgg
      181 gaaaggggtt ttgcgccggt cacgacttga aggagatgag gagtaatccg ggcagagaat
      241 tctacgagaa gacattcgat acctgctcaa ggctaatgat gtccatagtc ggatgcccga
      301 agcccgtcat tgcaatggtg cacggcgtcg caacggcggc cggatgtcag ttggtcgcca
      361 cgtgcgacct cgccgtggcc gacgagaacg ccaggttcgc cactcccggc gtgaacatcg
      421 gactcttctg ttccactccg atggtcgccc tctcgcggaa tgtctcgagg aagcacgcca
      481 tggagatgct gctcaccggg gattttatca ctgccccgag ggcttatgaa atagggctcg
      541 tcaaccgcgt tgtcccatcc ggggatctcg acctcataac tcacgagctc gccggtaaga
      601 tagcgtcaaa gtccccgctc acgctcagga taggcaaaaa ggctttttac gagcaggcgg
      661 accgtgacct tcgcggcgcg tatgagtact gcagccgcgt aatggtggaa aacatgatgg
      721 cccgggacgc tgaagagggc attgacgcgt ttttggagaa gcgcgagccc cattggaaag
      781 gcgtctgatc cagatcttaa tatttcctta acattactcc ggtaatgtct ttcagatttc
      841 cgttcccggg ctgaaaggcg gcgcatcgtt acatttatat ttcagtctcg tttgattata
      901 taattgttaa tacgggtaca tcatcggcga agcgatactc gcttgagcgg atgaaaacta
      961 tttatacggg tggaattatg cgtttaacct atggattgat catcgcggtt ttactttact
     1021 ttgcacaggc ggctgcgtct caggcgcaat cgaacagtgt ctcgctgccg gtcatcatgg
     1081 ggagcaagca ggtcctcgtc ataggcgaat cctacggaca taccgagtca gcggagttct
     1141 tttcgaaaac cgtaacggag tatctgaata atggaggatg cattaaagta gggcttgaaa
     1201 tatcctccga ccagcaggcg acgctcgaca gcgccatgaa aggtgaggtt cccgtctcgg
     1261 agcttaagat aaacgacatc gtggacagcc ccgcctatag aagaatgctc accgatttga
     1321 gcgggcaggt aagagccggg aagtgcctgt ccgtgagcgc catagacgcc ccggcttccg
     1381 ttcctgtaac acgtgacgca tggatggaaa agcaagttac ggctctcacg ggcgatacgc
     1441 cggtaattct tctcgtcggc aacgtcaggg cggtcaagga tacgggagcg ggagattccg
     1501 gcgggctcct cgccgagcgc atgagcggta aaatgcctgc cgtcgcgtct gtgcttcagt
     1561 actggacgcc cggacaatgc gagaaccgga cggtcgaata cataaacgcg gaagacgaaa
     1621 gggcgggggt ttatcttaag gaaacggtga aggacatgac gcccgtaatg cctgctacgc
     1681 cgacggctat cgcggacggc gttctcgtct ggagctgtga gtctgagaac gtgacggaga
     1741 agatgaatat cgacgacagc gggaaagggg agtccgtgaa agaccaggtc ggcgtaacta
     1801 tggaaaagac cgaaacggtc gtaagggacg agaaggcatt gaagaagata aagtggggta
     1861 tgaaaaacga gtatccagcc atcggaatga cgaaagacga agctcttcag gcgatggggg
     1921 agcccgaaaa taaagaagat gttaacggcg tcgaaaggtg gtcctaccag tgtttcgatg
     1981 aggacggata ctggcacaca tgtttcacgc tcgattttaa agacgatata gtggtcaagt
     2041 tcctcgacct cgaataaacc tgtcccggaa tgcgcaaggg cccgagcccg cgttcgtaat
     2101 cggtttccct tcagtgcgta tgtagaaaat taccgggccg cggaattcta ttctgcgggg
     2161 cctgcggatt cttcggcctt cgattccgct tctgccgggg gcagggttga gccgtttgtc
     2221 gccgcaatct gttcgtacct cgacctctcc ttgatcctgc tcgccttttt ggagcggccc
     2281 ctcaggtagt agagcttggc tttccggact ttacctttct tcttgacctc gatcttgtcg
     2341 ataagcggcg aatggagcgg gaatatcctc tcgacgccga cgccgtatga aacctttctt
     2401 actatgaaag tctcacgtac gccgccgttt ttcttggcga tgaccacgcc ttcgaatacc
     2461 tggatccttt ccctgtcgcc ctctttgatc ttgtaatgga cggctatggt atcgcctgaa
     2521 cggaattcag gcaggtccga cctcatcatg gatttttcaa gttcgagtat tacgttcatc
     2581 ttacttctcc tgtctcatat tgtacacgaa aacaatctgt ctagtattat cgccgccgcc
     2641 gatctcacgg aaaggtgatt gtatcctgta tatccgctga ccggcttgag cgtgtaatca
     2701 gcgccttgca ttatctcctc tgaaagtccc cagcctgttc cgaacacgag aagaaaagga
     2761 cagctgtctt caccagccat caggtccctc agctctccgt aaccgatcat gttattccgg
     2821 ggcctcgcgt ccgtaaccac aataacgggt tttctgccct cgatcttttc tatgtccgat
     2881 acggcgtccc cgagcgaatc gctgatctcc acgaattcca tcgcctcggc ccttgactta
     2941 ttgagcgccg gcccctcgcc ctccgtccag tgcctgagca cacgctctgc cagcttctgc
     3001 tgctccggat tgggctgaac gagataaaat ttctcgattc cgtatgtttt tccggccctg
     3061 gatatgtcgt ggacgtccag attagtgaac gccgtggtta taaccgtaag ccgattattg
     3121 tatacgggat aatggaccaa tgcaatatat gctttgaaat cgggccgtga ggtctttttt
     3181 aaatcattag cggcggttat atcgtcgagc gtgagcctcg ccctgtcgag gagctcgggt
     3241 ctccttaaat acgtcctctt tacgctctcc cttcttctcc attcatctat ttccttatgg
     3301 ttgcccgata gcaggacttc cgggatcttc ctccccatat attcctcggg cctcgtgtac
     3361 tggggatatt cgaggagcca gtccttaaat gaatcgctct ccggagacga tacgttcccg
     3421 aggaaccccg ggatgaacct cgccacgctg tcgacgataa cggcggccgc gtattccccg
     3481 cccgagagca cgtagtctcc gatcgagatc tccctgtcga cgtagatctc ccttacccta
     3541 tcgtcgatac cttcgtatct cccgcagatg atgataagct gatcgtatcc ggaaagctcc
     3601 cgcgcggttt tgtccgtgaa tatctcgccc gcgggagtcg taaggattac gagaggattc
     3661 gggcccttcc tctttgcgtt ctccaccgcc ttgcctatcg gcccgggttt catgagcatg
     3721 cctccaccgc ctccgtaggg agagtcgtcg accgtcctgt gcctgtcttc cgtgtaatcc
     3781 ctgatatcgt gcgttttcag ctccacgatg cccttctcgc tcgccttttt gaaaattccg
     3841 agcgagaaag gggattcgaa gaattcgggg aatatggtca gaatgtcgaa tctcatggtt
     3901 ttaactctgg gggcagcgct cgccggttat tctccggaaa gcagtc
//
LOCUS       DASSJH010000013         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_976729, whole genome shotgun sequence.
ACCESSION   DASSJH010000013 DASSJH010000000
VERSION     DASSJH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976729"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..852)
                     /locus_tag="VFJ67_00240"
     CDS             complement(82..852)
                     /locus_tag="VFJ67_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET7288835.1"
                     /translation="MKNIITLMTDFGLRDHYAGVMKGVILGINPDAVIVDITHEIRKY
                     HIYEAAFKLRGCYNYFPKGTIHVAVVDPGVGGPRRPLAAEAGGYYFVGPDNGIFSPVL
                     ESVDKSAVVEITRTEFMLENVSRTFHGRDIFAPAAAHLSNGVSILDLGRTLPQPVSID
                     VPTPSVKGDEIRGVVLYADSFGNLVTNVPGNMVVPGMAVYAGQYRIGPVKATYGDVEK
                     GNLLAIIGSSGLLEISVNQGSAREVLNKERVFVRIVRE"
     gene            1152..2033
                     /locus_tag="VFJ67_00245"
     CDS             1152..2033
                     /locus_tag="VFJ67_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7288836.1"
                     /translation="MELMEAIGTRRSIRFFKPWKEVEDWKIQRILQAARYASCQGNCG
                     STEAIVIDKRTYPADKFEQLIECASTFNEIQLRTAPVVIAWLVNMDAWYKELVESFAV
                     LFPARAITAAHGWTYKILTETTYPRLMSFPRDRAEDLLRIEAGQAIANALLAATELGL
                     GCCLLATGRKPAEFPKVLGVPENIVPLWLMAVGYAAETPGQRPRKRFDKLFHYNEYGT
                     PLKEDPIVKEQLRECRMIQPMDPLPGREEELRHICRMFGLKEDMPDMPKDKIKALYEK
                     DSIYYGEVPPGLEEKGV"
     gene            complement(2053..>3026)
                     /locus_tag="VFJ67_00250"
     CDS             complement(2053..>3026)
                     /locus_tag="VFJ67_00250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7288837.1"
                     /translation="LYPALSELRNHYADHTIDVLAEKRNSGIFAGCPYINNLLLYDRH
                     TLDTLYGVIKGGYDIVIDTEQWHRMTSALSYLTRAPERVGFATNERAELYSRRVRYSQ
                     DDYEAVSFLNLVSALTGEKYEFDGAKPFLPIASGLNGELGAAVSDFRAGKKAVLGIFT
                     GATVPERKWGSDKFAGLAGELSKENIGIVLLGGGQEIRDAGVFSRTREDGFALDLIGK
                     TPLRETASVISELDLFISADTGLMHIAYGVGTPTVSLFGAGIQKKWAPRGDKHIVINR
                     NEPCSPCTRFGYTPRCPYGVRCLAEIDVEEVKESVLELLRLTAHERE"
BASE COUNT          738 a          861 c          783 g          644 t
ORIGIN      
        1 cagaatagaa tgggaagcat agtcatgctt cacctggcta tagccctgcc aatgtaaacc
       61 gttaagggta gaggtgttaa ttcactccct tacgattcta acaaaaaccc gctccttatt
      121 gagaacctcc cgcgcgcttc cctgattgac cgatatctcg agcagccccg agctcccgat
      181 aatcgcaagg agattcccct tctccacatc cccgtaagtc gccttgacgg ggcctatacg
      241 atactggccc gcatatactg ccatgcccgg cacaaccatg tttcccggaa cgtttgtgac
      301 gagattgccg aaggaatcgg cgtacaaaac cacgcctcta atttcatcac ctttcacgga
      361 aggcgtgggg acgtcgatag agaccggctg ggggagagtc ctgccgagat cgagaatgct
      421 cactccattc gacagatgag ccgcagccgg agcgaagatg tcccttccgt gaaaggtcct
      481 gcttacgttc tcgagcatga attcggtcct cgtaatctct acgacagccg atttatcgac
      541 agattcgagc acgggcgaga atattccatt atcaggtccg acgaagtaat accccccggc
      601 ctcggccgcg agggggcgcc tcggtccccc gacaccgggg tcgacgactg cgacgtggat
      661 cgtccctttt gggaaatagt tataacagcc cctgagtttg aaagcagcct cgtatatatg
      721 gtacttcctg atttcgtgag tgatgtccac gatcacggca tccgggttta tgccgagtat
      781 cacacccttc atcacacccg cgtagtggtc tctcaacccg aagtccgtca ttaacgtgat
      841 tatattcttc atagccgttt gcccttttag ggaataatgc cgaattaccc cttaataaaa
      901 aatagcttaa taaacaacga ttgaaaacaa aattcacgat acagattact taggaacggg
      961 cgcgtgcagg gcacattccc cacgaccaaa ccggcgacct cgcgccttcc ctgttctcca
     1021 ccaaaaacgc gttcggctcc ccctgcggct cgaaacgcga aaaccgctta aactatgggt
     1081 tggtccgtgg ctaaatagcc ggttgacatc aacagacgat actgctttaa tgagttcagg
     1141 aggcaacgac tatggagcta atggaagcga tagggacgag aaggagcata agatttttca
     1201 aaccctggaa ggaagttgag gactggaaga tacagaggat acttcaagcg gctaggtacg
     1261 cgtcatgcca ggggaactgc ggctcgacgg aagcgatagt catagacaag aggacatatc
     1321 ccgccgacaa attcgagcag ctgatcgaat gtgcgtccac attcaatgag atacaattgc
     1381 gaacggcccc tgtagtgatc gcgtggctcg taaacatgga cgcgtggtat aaggagctcg
     1441 tcgagtcgtt cgccgtgctc ttcccggcaa gagccatcac cgccgctcac gggtggacgt
     1501 ataaaatcct aacggaaacc acttaccccc gcctcatgag cttccccagg gatagagcgg
     1561 aagacctgct gagaatagaa gcgggacagg caatcgcgaa cgccctttta gccgcgaccg
     1621 agctcggcct cggatgctgc ctcctcgcga cagggaggaa accggccgaa ttcccgaagg
     1681 tgctcggcgt ccccgagaac atcgtccctt tatggctgat ggccgtcgga tacgcagccg
     1741 agaccccggg acagagaccc cgtaaaaggt tcgacaagct ctttcactat aacgaatacg
     1801 gcactccgct aaaagaagac ccgatcgtaa aggaacagct cagagagtgc aggatgatcc
     1861 agcccatgga cccgctcccg gggagggaag aggagctgag acacatatgc aggatgttcg
     1921 gcctcaagga agacatgccg gacatgccca aggataagat aaaggcgctt tacgaaaagg
     1981 actccatata ctacggcgag gtcccgccgg gactcgagga aaagggagta tagaatcccg
     2041 gctacctata ccttactctc tttcgtgagc agtaagcctg aggagctcaa gaacggattc
     2101 cttaacttct tcaacgtcta tctctgcaag gcatctcact ccgtaagggc atctcggcgt
     2161 gtatccgaat cttgtgcagg gactgcaggg ctcgttcctg tttatcacaa tatgtttgtc
     2221 cccgcgcggg gcccacttct tttgtatgcc cgcgccgaat aaagacactg tcggcgttcc
     2281 gaccccgtat gcgatgtgca ttaaaccggt gtcagccgag atgaagagat cgagctccga
     2341 gataacggag gccgtttccc gaagcggcgt tttccctatg aggtccaggg cgaatccgtc
     2401 ctcccgcgtt cgggaaaaca cgcctgcgtc cctgatttcc tgcccgccgc ctaaaagaac
     2461 aatgcctata ttctctttcg acaattcccc cgcaagcccc gcgaatttat ccgatcccca
     2521 cttcctctcg gggaccgtag ccccggtgaa tattccgaga acggcctttt ttcctgccct
     2581 gaagtccgac actgccgcgc ctaattcacc gtttaaaccc gacgcgatcg gaagaaaagg
     2641 cttggcccca tcgaactcgt acttctcccc tgtaagagcc gatacgagat tgagaaagct
     2701 gaccgcctcg tagtcgtcct ggctgtatct cacccggcgg ctatagagct ccgccctctc
     2761 gttagtcgcg aagccgaccc tttccggggc gcgcgtcaga taggaaagag cggacgtcat
     2821 gcggtgccac tgctccgtgt ctatgacgat gtcgtagccg ccctttatca cgccgtagag
     2881 cgtatccaga gtatgcctgt cgtaaagtag gaggttattt atatacgggc atccggcgaa
     2941 tatcccggag ttccttttct cggcaagcac gtcgatcgtg tggtccgcgt aatgattcct
     3001 gagctcggaa agagcgggat agagga
//
LOCUS       DASSJH010000014         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_868359, whole genome shotgun sequence.
ACCESSION   DASSJH010000014 DASSJH010000000
VERSION     DASSJH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868359"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..2545
                     /locus_tag="VFJ67_00255"
     CDS             50..2545
                     /locus_tag="VFJ67_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7288838.1"
                     /translation="MGNERKTIDRLTREIEDLRLGLSSHKDREEELERRIEQLTVCFR
                     SIGDGVIVSSSDGTIILINRKAEELTGWSAEEAVGKPLQEVLRLHHKDSPGTPIAVAG
                     LVLETGEKLGLPRDTVLVTRSGKTHFVSASIAPVNYEQTDFRGVVVAFRDISRLRETE
                     LGLIENEKKYRTIIENSYDLIYEVDSSGNILFVNPMCAELTGYTQSELQGRNAFDFMH
                     PDDRPQAMAVFMRAVKNYTTEKATFRALHKSGEYQWLECVGKPYLTASGEIRGVIITR
                     DISERKRREEELLALNTLMKAVHKFLDLREVYDVALDVIMNMENMDMAMIYLVDPEKR
                     EAVIQASRNLPEFYLKRAGRIPCPKGLTWKIIESGAIKNIEDAQKDPEIGDAGRELGH
                     HSLLGIPIFQGTEVIGVIWFLIYKERKFSEHEVHLLSAMGDQIAIAVAKAKMLEEIKS
                     AQERLVQSEKLASLGQMVSSIAHEINNPLTPIMGYSQRLLTKPGLDKGEKKSLEIINA
                     SAQRVAKIIENLLSFSRNYRPYRTYQDINALVREAIESRGQQLKLGNIEVIADYGEGI
                     PRTMVDSDQVRQVFTNIILNAEQAIGGHGPGGRLRIATGLKNGNAIKITFEDDGPGFG
                     KEALGKLFDPFFTTKEPGKGTGLGLAVAYGIVKEHGGEIRAENREGKGALIIVELPVL
                     EEASPPEVRSEKKQARRSGRRANEKRILVIEDEELVVDLIKGVLEHDDMTIDVARDGE
                     EALAKAVSGEYDLIVCDIKMPVMGGIAFYNKINIINPVIARKIVFITGDPSSETMEFL
                     NTTGKEYITKPFKVEKFRERVCELLAPSVRSDA"
     gene            complement(2690..2839)
                     /locus_tag="VFJ67_00260"
                     /pseudo
     CDS             complement(2690..2839)
                     /locus_tag="VFJ67_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013694520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          874 a          754 c          929 g          604 t
ORIGIN      
        1 gtctgtaata aaccggaaac gattcgcccg gtatcaaaag ggaaaataag tggggaacga
       61 acgaaaaacc atagatcggc tgacaagaga gatcgaagac ctccgtctcg gcttatccag
      121 tcataaagac agagaggaag agcttgaacg ccgaatagag cagctcaccg tatgcttcag
      181 gagcataggc gacggcgtca tcgtttcatc ttctgacggg actataatcc ttatcaaccg
      241 gaaagcagaa gagcttacgg gctggagcgc ggaagaagcg gtcggtaagc cgctgcagga
      301 agttttgcgt ctgcatcata aagacagccc cggtaccccg atcgcggttg ccgggctcgt
      361 tctcgaaacc ggggagaagc tgggcctgcc gagggatacc gtgctcgtca cccgcagcgg
      421 gaagacacat ttcgtctcgg cgagcatcgc cccggtgaac tacgagcaga cggacttcag
      481 gggagtggtc gttgcgttcc gtgatataag caggctgagg gaaacggaac tgggccttat
      541 agagaacgag aaaaagtacc gcacgatcat cgagaacagc tacgacctca tctacgaggt
      601 ggattcatcc gggaatatac tgttcgtgaa ccctatgtgc gcggagctta cgggttacac
      661 gcagtccgag ctccagggga gaaacgcctt cgatttcatg caccccgatg acaggccgca
      721 ggccatggct gttttcatgc gcgccgtgaa gaattacacg acggaaaaag ctactttcag
      781 ggcgctccac aagtcgggtg aataccagtg gctcgaatgc gtggggaagc cttatttgac
      841 ggcttcggga gagatacgcg gcgttatcat cacgagggat atctctgaac ggaaaaggag
      901 ggaggaagag ctcctggcgc tcaatacgct gatgaaggcc gtccacaagt tcctcgacct
      961 cagggaggtt tacgacgtag ctctcgacgt cataatgaac atggaaaaca tggacatggc
     1021 catgatctat ctggtcgatc ctgagaagag ggaggctgtg attcaggcaa gcaggaatct
     1081 ccccgagttc tatctgaaga gggccgggag aataccctgc ccaaaggggc tgacgtggaa
     1141 gataatagag tcgggagcca tcaagaatat agaagacgcg cagaaagacc ccgagatcgg
     1201 cgacgctggc cgggagctcg gtcatcacag cctgctgggt atcccgatat tccagggaac
     1261 agaagtgata ggcgtaatat ggttcctcat ctataaggag cgcaagttct cggagcacga
     1321 ggtgcatctc ctttccgcga tgggcgatca gatagcgatt gcggtagcca aggcgaagat
     1381 gctcgaagag ataaagtccg cccaggagag gctcgtacaa tcggagaaac tggcgtccct
     1441 cggccagatg gtctcgagca tcgcgcatga aataaacaac cccctcactc ctataatggg
     1501 atattcgcag aggctcctga cgaaacccgg actggacaag ggagagaaga agtcgctcga
     1561 aatcataaat gcgtccgcgc agagggtcgc aaagataatc gagaacctcc tctcgttctc
     1621 aaggaattac aggccgtaca ggacgtatca ggatataaat gcgctcgtca gggaggctat
     1681 cgagagcagg ggccagcagc tgaagctcgg gaacatcgag gtgatcgctg actacggcga
     1741 aggcatcccc aggacgatgg tcgattccga ccaggtaagg caggttttca ccaatatcat
     1801 tctcaacgcc gaacaggcta ttggcggtca cgggcccggg gggcgtctca ggattgcgac
     1861 cggacttaag aacggaaacg caataaaaat cacgttcgag gatgacggtc ccggtttcgg
     1921 gaaagaagcg ctcgggaagc tgttcgatcc gttcttcacg acgaaagagc ctggaaaggg
     1981 caccgggctc ggtctcgccg tcgcgtacgg gatcgtaaaa gaacacggcg gcgaaatacg
     2041 cgcggaaaac cgggagggaa agggggcgtt aataatcgtc gagctgccgg tattggagga
     2101 ggcgtcaccg cccgaggtca gatcggaaaa gaaacaggct cggaggagcg gaaggagggc
     2161 caacgagaag cggattctgg tcatcgagga cgaagagctt gtcgtcgatc tgataaaggg
     2221 cgtcctcgag cacgatgata tgaccatcga tgtcgcaagg gacggagaag aagcgctcgc
     2281 aaaagccgtt tccggtgaat acgaccttat agtctgcgac ataaagatgc cggtaatggg
     2341 agggatcgcg ttctataata agatcaacat cataaacccg gttatagcca ggaagattgt
     2401 tttcataacc ggcgacccga gcagtgaaac catggaattt ctcaacacga cgggtaagga
     2461 atacattacc aagcctttta aagtcgaaaa attcagagag cgcgtgtgcg agctgttggc
     2521 gccctctgtg agaagcgacg cttaatatac ctccgtgtga acatcattgt aacttgaatt
     2581 gacggctccg ttcgaaatcg actttcacgt ttgtgaagct gacataactt tgcggaaacg
     2641 aaccagagcg gagatcaaaa aagccctccc agtatcacca cggccgcgaa gtcgcgcccg
     2701 agaggagcga catgacgagg aacaccggaa tcgcgaggag cccgaactgg ggcatcgaac
     2761 gagcgatagt ctcgaggaat atgcccagcg atgatacaga gaagaggtgg gcgatcgtcc
     2821 cggctacgaa taattgaagc tcgcaatttc tccgccgtcc cgcatacggg cttgagccgc
     2881 gtgtgagaag gatcgataac agtgaggcat tcaagcacat ttcggtcttg cctgagaaga
     2941 attcaattct tgtactattt tctgacagac gacacaatag ctcgccgaaa aatcaacgat
     3001 catgggatgc cgattattgg catattactt tcatgattta cggacggtgg aatatcaggg
     3061 tgtaaaaata caataaatta atccaaggag gtaaggagat gaaaaaggcc aaatggctct
     3121 ttatatttgt gctggcgctc ttcacggctg caccgtcgtt c
//
LOCUS       DASSJH010000015         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1084964, whole genome shotgun sequence.
ACCESSION   DASSJH010000015 DASSJH010000000
VERSION     DASSJH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084964"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFJ67_00265"
     CDS             <1..939
                     /locus_tag="VFJ67_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288839.1"
                     /translation="GFRNPSVPERADIVALGDSHTYGVNVKSENAWPAQLGRMSGMTV
                     YNLGVGGYGPLQFYYLMDKALSFKPRHIILGLYPANDLSDVCKLIDESEYWMDWAKKR
                     GYDVGVCAGSESFFGLVNRTLSGLHVYWMVASGIKRVNESVNFGDALEVREGDNKTIM
                     KYATISSHMKKMDLSREKVALGLAVTEDVIREMKSKADAEGVELGVVLIPSKEIAFYD
                     YLKANGYEIPDDYEKLVVNEDEITGELAGFLAGLGIDYADARPYAVNEVGKSITAYSP
                     TDDGHPLEAGYGAYARAVYDIIITDRVPENRRVRVK"
     gene            complement(963..1649)
                     /locus_tag="VFJ67_00270"
     CDS             complement(963..1649)
                     /locus_tag="VFJ67_00270"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HET7288840.1"
                     /translation="MTTSEYSRKNREILAVCLATVAGYVDAYGFLTFSTFVSFMSGNT
                     THAGYNIGLGLYHAALPAALAIISFVAGVFSGTLLTRSRARRSRRALYSSITSLIALY
                     IIASHLGAVTTFPGIVLLSFTMGVMNTALSRVGKQTVNLTFVTGTLSQIGGHLALAAR
                     REPLGDGEGAGDTHLRRALMLSVIWTGFLGGAVLGGLATSLLREWMLVIPLLIMLTLA
                     LIGQARYAED"
     gene            complement(1774..2520)
                     /locus_tag="VFJ67_00275"
     CDS             complement(1774..2520)
                     /locus_tag="VFJ67_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7288841.1"
                     /translation="MRIPMKIENKIAIVTGASSGIGRALAGLLSEKGARVALVARSRD
                     KLEALSKKIPHSLAVPADMTVRADIERAVNKTIDHFGGVDMLVNNAGRGYDAPVESID
                     IEEYRRTLELNLIGPVIAMQLVIPEMRKRGGGSIVNVSSGTALMHLTNMSPYSSVKRA
                     LAGISLTAREELKKDNIIVSVLYPFITDTDFEKNTVKGDVVDESPEAGQKFTPDTPEY
                     IARRICEGIEAEEAEIFAHDWMKDLSGKAG"
     gene            2695..3306
                     /locus_tag="VFJ67_00280"
     CDS             2695..3306
                     /locus_tag="VFJ67_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7288842.1"
                     /translation="MGVKERREREKLAVRKEILDAARELFLKEGYESVSMRKIADKIE
                     YSPTTIYLYFDDKAQLVHSLCEEAFVKLVKMFETLGGDLSDPVSALKKCGRAYIEFGL
                     KYPNDYKVTFMLNLRPDKSPEEYLREDSMGMRAYGYLRKIVEECVRQGKFHGADVETT
                     TQTVWIVVHGVTSLLIAHPKFPWVDRECLIDFTIEKIVNGLSG"
     gene            3360..>4007
                     /locus_tag="VFJ67_00285"
     CDS             3360..>4007
                     /locus_tag="VFJ67_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7288843.1"
                     /translation="MSKTIAITGCSSGFGRATALHMASRGWRVFASVRRKADGDSLLS
                     EASERGSAEFITPLVCDITNAVQVSAMGRSIAEAAPGLDALLNNAGTAFPAPLELLPL
                     DALRSQLEVNLIGHLSVTQTLLPLLKAARGTIINVTSVGGRIALPLIGAYNASKFAME
                     AVSDVLRFELAPFGVRVVVIEPGTSATSIWETSVNRANELLGERADVKPYIPLIDS"
BASE COUNT          890 a         1074 c         1198 g          845 t
ORIGIN      
        1 gggttcagga atccctcggt cccggagcgg gccgatatag tcgcgttagg cgactcgcac
       61 acctacggcg tgaacgtgaa gtccgagaat gcgtggcccg cgcagctcgg ccgcatgtcc
      121 ggcatgaccg tttacaacct cggcgtaggg ggatacggcc ctctccaatt ttactatctg
      181 atggataagg ccctctcgtt caagccacgg cacataatcc tggggctcta tccggccaac
      241 gacctgagcg acgtatgcaa gctgatcgac gaatcggaat actggatgga ttgggccaaa
      301 aagcgcggct acgatgttgg cgtctgcgcg ggctcggaga gttttttcgg ccttgtaaac
      361 cggacgctct cgggcctcca cgtttactgg atggtcgctt ccgggataaa gcgcgtgaac
      421 gagagcgtga atttcggaga cgcgctcgaa gtcagggaag gggacaataa aacgatcatg
      481 aaatacgcga caataagctc tcacatgaaa aagatggacc tctcgcgcga gaaagtcgca
      541 ctaggcctcg ccgttaccga ggacgtgatc cgcgaaatga agagcaaagc cgacgcggaa
      601 ggcgtggagc tcggcgtggt actcataccc tcgaaagaga tcgcgttcta cgattactta
      661 aaggcgaacg gctacgagat accggacgat tacgaaaagc tggtagttaa cgaagacgag
      721 atcacgggag agctcgcggg ctttctcgcc gggctcggga tagattacgc cgacgcgcgg
      781 ccttatgccg ttaacgaagt gggaaaatcc ataaccgcct attcgccgac cgacgacggc
      841 cacccgctcg aagcaggtta cggggcctac gcccgggccg tgtacgacat aatcattacc
      901 gacagggtcc cggaaaacag gcgcgtgaga gtcaagtaag taacggcgct tcaaatccct
      961 cgtcagtcct ccgcgtatcg ggcctgaccg atcaaagcga gggtgagcat tatgaggagc
     1021 gggatgacga gcatccactc gcggaggaga gaagtcgcta acccgccgag tacggcgcct
     1081 ccgagaaagc ccgtccatat taccgagagc ataagtgcgc ggcggaggtg agtatcgccc
     1141 gcgccttcgc cgtcgccgag cggctcgcgc ctggctgcga gcgccaggtg tcccccgatc
     1201 tggctcagcg tacccgtaac gaatgtgagg ttcacggtct gcttccccac gcgcgagagc
     1261 gccgtgttca ttacgcccat cgtgaagctg agcaatacga tccctggaaa cgtcgtgacg
     1321 gcgcccaggt gcgaggctat gatgtagagg gcgatgaggg acgtgatcga ggaatagagc
     1381 gcccggcggg accggcgcgc gcgcgatctg gtcaggagcg tgcccgagaa gacgcccgcg
     1441 acgaacgaaa ttatagcgag ggcagccggc agggcggcat ggtacaagcc gagtccgatg
     1501 ttgtagcccg catgcgtggt gttgccgctc atgaacgaaa cgaaggtgct gaaggtgagg
     1561 aagccgtaag cgtccacgta acccgcgact gtcgcgagac agacggcgag gatttcacgg
     1621 ttttttctcg aatattcgga tgtcgtcact gctttcaccc ggctttgaat ctggataatc
     1681 gccggcacaa aattctacaa tttgacaagg gaaaacggaa tacccgccgt cggggttatt
     1741 ttcacgcatc gatacgaaat catccgttag gttttatccg gctttaccag acaggtcctt
     1801 catccagtca tgggcgaata tttcggcttc ttccgcttcg atgccttcgc atattctccg
     1861 cgctatgtat tcgggggtat cgggggtgaa cttttgcccg gcttccgggc tttcgtcaac
     1921 gacgtcgcct ttgaccgtat ttttctcgaa atccgtgtcg gtgatgaaag gatataagac
     1981 cgagactatg atattgtcct ttttcaattc ctctctcgcg gtaagggaaa tgcccgcaag
     2041 cgcccgtttc actgaggaat aagggctcat gttcgtaagg tgcatcagcg ccgtgcccga
     2101 gctcacgttg acgatcgatc ccccgccgcg ttttctcatt tccgggatca cgagctgcat
     2161 ggctattacg gggccgatca ggttcaattc gagcgtgcgg cggtattctt cgatatcaat
     2221 ggattccacg ggggcgtcat aacctctccc cgcgttgttg acgagcatat cgactccccc
     2281 gaaatgatct atcgttttat tcacggccct ctctatgtcc gcccgcaccg tcatgtcggc
     2341 cgggacggcg agcgagtgcg gaatcttttt agagagggct tccagtttat ctctcgaccg
     2401 ggcgacgagg gcgaccctcg cgcccttttc ggaaaggagc ccggcaaggg cgcgtcctat
     2461 tcccgaggac gcgccggtta cgatagcgat cttgttctcg atcttcatgg ggattctcac
     2521 caagagttta tcctgtgtct gcaataaccg catccgtcat gctatggaat atagcgggtc
     2581 cgatggccgt tcccgcgggc atatgatccg cgttaaaaaa aatccttgac ttcatgtacg
     2641 cccttaatat acttaacact gttaagtata atactggagt taaggggacc ctgcatggga
     2701 gttaaagaac gacgggaacg ggaaaagctg gccgttagga aggaaattct ggacgcggcg
     2761 cgggagctct tcttaaagga aggttatgaa agtgtttcga tgcgtaagat agccgataag
     2821 atcgagtact cacctaccac tatatatcta tatttcgacg ataaggctca gcttgtccat
     2881 tctttgtgtg aagaagcgtt cgtaaagtta gtgaagatgt tcgagaccct tggcggggat
     2941 ttgagtgatc cggtctcggc cctgaagaaa tgcggacggg cttatataga gttcgggctc
     3001 aaatacccga acgactacaa ggtgacgttc atgctcaatc tcagaccgga caagtcgccc
     3061 gaagagtacc tccgcgagga ctcgatgggt atgcgggctt acggttatct gaggaagatc
     3121 gtggaggaat gtgtgaggca ggggaaattc cacggcgcgg acgtggaaac cacgacacag
     3181 accgtttgga tcgtagttca cggagtcact tcgctcctca tagcccatcc gaaattccca
     3241 tgggtcgaca gggaatgtct gatcgatttt acgatagaga agatagtaaa cggcctcagc
     3301 ggctgaatcc ctgacggcgg aatgcatcat aggatcgctt tcgccgggga gagtcgagaa
     3361 tgtcaaagac aatagcaatc actggctgct cttcgggctt cgggcgtgcg acagccctcc
     3421 atatggcaag ccggggctgg agggtgttcg catccgtgcg caggaaagcg gacggtgata
     3481 gcctgctctc cgaggcatcg gagcggggct cggccgaatt cattacgccg ctcgtctgcg
     3541 atataacgaa cgcggtgcag gtgtcggcga tgggccgctc tatcgccgaa gcggcgccgg
     3601 ggctggacgc cttactcaac aacgctggca ccgcgtttcc cgctccgctc gagctcttgc
     3661 cgctcgacgc tctcaggtct cagttggagg tgaacttaat cgggcacttg agcgtgacac
     3721 agacgctcct tcctctcctg aaagccgccc gcggcaccat aatcaatgta acgagcgtcg
     3781 gaggacggat cgctctgcct cttatcggcg cgtacaatgc cagcaagttt gcgatggaag
     3841 cggtgagcga cgtgctgagg ttcgagctcg ccccgttcgg cgtgcgagtg gtggtgatag
     3901 agcccggcac gagcgcgact tcgatctggg agacgagcgt caaccgggcg aatgagctgc
     3961 ttggggagcg cgcggacgtt aaaccgtaca tcccgctcat cgactct
//
LOCUS       DASSJH010000016         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_761064, whole genome shotgun sequence.
ACCESSION   DASSJH010000016 DASSJH010000000
VERSION     DASSJH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761064"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFJ67_00290"
     CDS             <1..641
                     /locus_tag="VFJ67_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288844.1"
                     /translation="QNGIFRIHALTFSFRDTAVIFSANAGGGKSTMALKMLESDVLKI
                     ISDDEALVNASGRLLPFPIRIGTLDRGRISSVPDEYVYELDRMEFGRKFFVDLDYWRK
                     RLETRELDKKIYFVAQRLINGDPCIEKISGYGIFKSLIGGAIIGFGLYQGMEFVFNNP
                     LRDTLSLLSVFLRRTASAIRFARETTGYRILLSRDTEKNFRKFEEFIVNLRM"
     gene            645..1604
                     /locus_tag="VFJ67_00295"
     CDS             645..1604
                     /locus_tag="VFJ67_00295"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7288845.1"
                     /translation="MTNNNQIIDISLVVLTYNRKESLRRCLGSVSSMKKGDITFEVIV
                     VDDGSETDNRPVIDQFRNSLDIRYFKKANEGVASTRNFGLSKSRGKLVGFIADDYTLP
                     DSYLTDVIEFFENHPEAMIITHNIRPTGPSVFRYVQSIYFQMTLIQMLDERDLNKDVV
                     TSFHLPPSRGAVFRREIFETLGNFNEDFLTGEDGEFGMRMASRGIPVYFFPRKFIDHY
                     EDKYLKGYLDQRIRYGGSFYRAVLARDGERTRKPTIYGISKSTIQKYYSWLKLSGAIG
                     REAEYLLLSPFIALFLIFYYAGFYSESRKTVHSYESTQSRISS"
     gene            1642..>2607
                     /locus_tag="VFJ67_00300"
     CDS             1642..>2607
                     /locus_tag="VFJ67_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET7288846.1"
                     /translation="MSLKAFLFKISRSKGGGIIGRWAYILCSRFVSLVFRLTPEIRSA
                     YLAGSMSNGDIAPGLSDIDFVIVIKDLSALDEYRLVNRLEKALRYLLPPFGKDKLGTH
                     VLIYSAGEWALVGDLFSGKSYGQPRKLFQKDEIIYSHTFGRIVKGLHHFYKAHWRLEA
                     IQQNLHLPADNGLERALRERVLERSFQSIENGFREVLEYDETPASYMSLRNEIRKLMS
                     SADKTDSLYETILIRLLHFFDMSAGICFPDSPREEREDIPMKASVNTEFEKQGLKEFI
                     TGVPEAIEREARTHSILFSRMMYYDIYLYDPAYYETSEKLMGCYSR"
BASE COUNT          727 a          579 c          663 g          638 t
ORIGIN      
        1 atcagaacgg tattttcagg attcacgccc tcaccttctc attccgggac accgcggtca
       61 tattctcggc aaacgcgggc ggaggaaagt cgacaatggc tctcaagatg cttgaaagcg
      121 acgtcttgaa aatcatttcc gacgatgagg cgctcgtgaa cgcttcgggc cgattattgc
      181 cattccctat caggatcgga actctcgacc gcgggagaat cagctcggta ccggacgaat
      241 atgtttacga attagacagg atggagttcg gacgcaaatt cttcgtcgat ctggattact
      301 ggcgaaaacg cctggaaaca agagagctcg acaaaaaaat ttatttcgtc gcccagagac
      361 tgattaacgg agatccatgt atcgaaaaga tatccgggta cggtatattt aaaagcctga
      421 tcgggggggc tattatcggc ttcggcctgt atcagggtat ggaatttgtt ttcaacaacc
      481 cgctaagaga taccctgtcg cttctatctg tttttttaag aagaacagcc tcggcaataa
      541 gattcgccag agaaactacc ggataccgaa tactattatc gagagacacc gagaagaatt
      601 tcagaaaatt cgaggaattt atagttaatc tccggatgtg agccatgacg aacaataacc
      661 agataatcga tatatcgctc gtagtgctga cttataaccg taaggaatct ctgaggaggt
      721 gtctcgggtc cgtatccagc atgaaaaagg gagacataac attcgaggtc atagtagtgg
      781 atgacgggtc tgaaacagac aaccgtcccg taatagatca attcaggaat tccctcgata
      841 tcagatattt caagaaggca aacgaaggcg tagcgtcgac gaggaacttc ggcctgagca
      901 aatcgagggg aaagctcgta ggatttatcg ccgatgacta tacactgccg gacagttatc
      961 tgacggatgt gattgagttt tttgagaacc atccggaggc gatgataatc acgcacaata
     1021 taagacccac gggtccgagc gtgttcagat acgtgcagag tatatatttt cagatgacat
     1081 tgattcagat gctggacgaa cgggacctca acaaggacgt ggtcacgagc tttcacctgc
     1141 cgccgtcgcg aggagcggtt ttcaggagag agattttcga gaccctcggg aattttaacg
     1201 aggattttct taccggcgaa gatggagaat tcggcatgag gatggcatcc cggggcatac
     1261 ctgtatattt cttccccagg aagtttatcg atcattacga ggataaatat cttaagggct
     1321 acctcgacca gaggatcagg tacggcggca gtttttacag ggcggtgctg gcccgtgacg
     1381 gggaaagaac gagaaagcct acaatatacg gaatttccaa atcgacaatc caaaaatact
     1441 attcctggtt aaagttatcg ggcgcgatag gcagggaagc cgaatatctt cttttatccc
     1501 ctttcatcgc tcttttcctg attttttatt acgcgggctt ttattcggag tcgcggaaga
     1561 ccgtacattc atacgagtct acacagtcgc ggattagcag ttgaaccgta tcaccaattt
     1621 agacattcga tattcaggga aatgagcctc aaagcctttt tatttaaaat cagccggtca
     1681 aaggggggcg ggataatcgg aagatgggca tatatattgt gctcgcgatt cgtatctctg
     1741 gtcttccgtc tgacccccga aatacgctcg gcatacctcg ccggtagcat gtcgaacgga
     1801 gacatcgcac ccgggttgag cgacatcgat tttgttatcg tgatcaagga ccttagcgcc
     1861 cttgacgaat acaggctcgt taatagactc gaaaaagcac tcaggtatct gctgcccccc
     1921 tttggaaagg ataaattggg gactcacgtt ctgatttact cggccggcga atgggctctt
     1981 gtgggtgatc ttttttccgg taagagctac ggacagccgc ggaagttatt tcaaaaggat
     2041 gagataatct acagccatac attcgggaga atcgtgaaag ggctgcacca cttttacaaa
     2101 gcgcactgga ggctcgaggc cattcagcag aatctgcatc ttccggccga taacggacta
     2161 gagcgcgcgc tcagggagag agttctggaa agaagctttc aatcgataga aaacggtttc
     2221 cgcgaggttc tggagtacga cgaaacgccc gcttcatata tgagtttgag aaatgagata
     2281 agaaagctaa tgagcagcgc agataaaacc gactcccttt atgagacgat actaatcaga
     2341 ctcctccatt tttttgacat gtcggcgggt atctgctttc cggattcccc gcgggaggaa
     2401 cgcgaagata tacccatgaa ggcttctgta aatacggaat ttgagaagca gggattgaaa
     2461 gaattcataa cgggagttcc cgaagccatc gagcgcgaag cgcgaacaca ttccatattg
     2521 ttctcacgca tgatgtatta tgacatttat ctctatgacc ctgcgtacta tgaaacttcg
     2581 gaaaaactca tggggtgtta cagccgg
//
LOCUS       DASSJH010000017         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_328561, whole genome shotgun sequence.
ACCESSION   DASSJH010000017 DASSJH010000000
VERSION     DASSJH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328561"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VFJ67_00305"
     CDS             complement(<1..1217)
                     /locus_tag="VFJ67_00305"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HET7288847.1"
                     /translation="MKLTENAKKVLEARYLRRNDEGDVIETPDELLKRVAGAVSAAEL
                     QYGDPSSAQMWEERFYDMLSTLRFLPNSPTLMNAGTPLGQLSACFVLPVNDSIEDIFD
                     AVKNMALIQRTGGGTGFSFSKLRPKGSVVSSTGGESSGPVSFMKIFDSATENIKQGGR
                     RRGANMGVLRVDHPDILEFITAKLEEGELRNFNLSVGITDAFMDALERDAECELKDPR
                     SGEVSTTLRAKDVFQKIAECAWRTGDPGALFLDTINRSHPLRGLGEIESTNPCGEVPL
                     LPYESCNLASVNLSAMLRDNEATVDWERLGETVRNGVRFLDDVIDVNRFPVGETAAMT
                     RGNRKIGLGVMGFAEMLIKLGISYNSPEAADFAGKLMSFIYDEAFRASTELAGKRGVF
                     PNWEKSVYAKRGVG"
     gene            complement(1319..2764)
                     /locus_tag="VFJ67_00310"
     CDS             complement(1319..2764)
                     /locus_tag="VFJ67_00310"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET7288848.1"
                     /translation="MNDRIEVRKIHDYLWEIPKTGGMLVPGHVYTTESMLEAIREEKA
                     LEQVMNVAYLPGIQKYSIAMPDIHWGYGFPIGGVAAFDAEEGVISPGGVGYDINCGVR
                     LMRSSLTEEEVTPHLKELVTEIFRRVPCGLGEGGKIKLTREDYGRIISKGIGWAIENG
                     YGDREDAQRVEDYGTFPGGDPSAVSERAYERGKDEIGTLGSGNHFLEIDVVDEVFIPS
                     VANSFGLFKGQVCVLIHCGSRGFGHQICTDYIDVMLSYMRKKNLELPDRQLACAHINS
                     EEGRRYLSAFAAAVNYAWVNRQVIMSFVRDSFYSVFGIGPRELGGSLVYDVSHNIAKF
                     ETHKVNGERKKVCVHRKGATRAFPKGHPLIPEIDKDVGHPVFIPGDMGRASFVLVGTE
                     AALDETFGSSCHGAGRVLSRTKALKLGRGRDIISELGERGIIVMARGRKTVFEEMPEA
                     YKPIEDVVDVVHNAGIANKVARLRPIGVVKG"
     gene            complement(2827..3375)
                     /gene="raiA"
                     /locus_tag="VFJ67_00315"
     CDS             complement(2827..3375)
                     /gene="raiA"
                     /locus_tag="VFJ67_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET7288849.1"
                     /translation="MKIELIGKGIEISEDLRSLTEHGLSKLERHLKEMGEGEVEAVVT
                     FTVEKHRERHRVDIDLYLKTPGGGALHAWEESNDTFMSLEFTLDDIDKQLNRLKDRRI
                     EVRKEIAREKAKRKNEEPPVREAGLISEEKLSIEKPLTTEEALMVLQDEDRFFLVFRN
                     KDTGDLNVVYRKKSGKYGLVAP"
BASE COUNT          660 a         1121 c          851 g          834 t
ORIGIN      
        1 ccgacgccgc gcttcgcata aacgctcttc tcccagttgg gaaacacgcc cctcttcccc
       61 gcgagctcag tcgaagcccg gaaggcttcg tcatagatga aggacatcag cttccccgcg
      121 aaatccgcag cctcgggaga attataggat atgccgagct tgataagcat ttcggcgaaa
      181 cccataacgc ctaatccgat ctttctgttt cctctcgtca tcgcggccgt ctcccccaca
      241 ggaaacctgt tcacgtcaat cacatcgtcg aggaaacgca cgccgttcct taccgtttcg
      301 cctaatctct cccagtctac ggttgcttca ttatcacgga gcatcgccga aaggttcacg
      361 gaggcaaggt tacagctctc gtagggcagc agaggaacct cgccgcacgg gttcgtggac
      421 tcgatctcgc caaggcccct cagggggtgc gatctgttaa tcgtgtcgag gaagagcgct
      481 ccgggatccc ccgtcctcca cgcgcactcg gctatctttt gaaacacatc tttagcgcgg
      541 agtgtggtcg atacctcccc cgacctcggg tcctttagct cgcattccgc gtccctttcg
      601 agggcgtcca tgaaggcatc cgttattccg acggagaggt tgaagtttct gagctccccc
      661 tcctcgagct tggccgtgat gaattcgaga atatccgggt ggtccaccct gagaaccccc
      721 atatttgcgc cccttctcct cccgccctgc tttatattct ctgtcgcgct gtcgaatatc
      781 ttcatgaacg acaccgggcc agaagactcc ccgcccgtgc tcgaaaccac tgagcctttc
      841 ggccggagtt ttgagaagga aaagcccgtg cccccgcccg tcctttgtat cagagccata
      901 tttttcacgg cgtcgaaaat atcctcgatc gaatcgttca cagggaggac gaagcacgcg
      961 ctaagctgac cgagcggggt gccagcgttc attagtgtcg gcgaattggg aagaaagcgg
     1021 agcgtgctaa gcatatcgta aaaccgctct tcccacatct gagccgagga cgggtctccg
     1081 tattgaagct cggccgccga gaccgctccc gcgacccttt tcagaagctc gtccggggtc
     1141 tcgatcacat ccccctcgtc gttcctcctg agatagcgcg cctcgagcac ctttttagcg
     1201 ttttcggtta gtttcaacaa tatctccgga ttgaataagt ataggataga aaaaacgtca
     1261 gtaatagtga atccgcaggc actccggacg gacggccttc agtatttccg ggcggctctc
     1321 accccttcac gacgcctatt ggcctcaggc gggcgacttt attcgctatg cccgcgttgt
     1381 ggacgacatc gacgacgtcc tctatcggtt tataggcctc cggcatttcc tcgaatacgg
     1441 tcttcctgcc tctcgccatg acaattattc ccctctctcc gagctcggag atgatgtccc
     1501 tccccctacc gagcttgagc gccttcgtcc gcgagagaac gcggcccgcc ccgtgacagc
     1561 tcgagccgaa cgtttcgtcg agcgcggcct ccgtaccgac taatacgaac gacgccctgc
     1621 ccatgtcccc gggtatgaac accgggtggc ctacgtcttt atctatttca ggtatgaggg
     1681 gatgaccttt gggaaaggcc ctcgtcgcac ccttcctgtg gacgcacacc tttttcctct
     1741 ccccgttcac tttatgcgtc tcgaacttgg cgatgttgtg gctcacgtcg taaacgaggc
     1801 tcccgccgag ctccctcggc cctatgccga acacggagta gaacgagtcc ctcacgaagg
     1861 acatgatcac ctgcctgttc acccaggcat agttcacggc ggccgcgaac gccgagaggt
     1921 acctcctccc ctcttccgag ttgatgtggg cgcaggcgag ctgtctatcg ggcagctcga
     1981 ggttcttctt cctcatgtag gagagcatga cgtctatgta gtccgtgcat atctgatggc
     2041 cgaagcccct cgacccgcag tgaatgagca cgcacacctg acctttgaaa aggccgaagg
     2101 agtttgcaac ggacgggatg aagacctcgt ccactacgtc gatctcgagg aagtggtttc
     2161 ccgagccgag tgttccgatc tcgtccttcc ctctctcata agccctctcg ctcaccgctg
     2221 acgggtcgcc gcctgggaac gttccatagt cctcgaccct ttgggcgtcc tccctgtcgc
     2281 cgtacccgtt ctctatagcc cacccgatcc ccttcgatat tatccgcccg tagtcctccc
     2341 gggtgagttt gatctttccg ccctcgccaa ggccgcaggg tacgcggcgg aatatctccg
     2401 tgaccagttc tttcagatga ggcgtcacct cctcttccgt caagctcgac ctcatgagcc
     2461 taacgccgca gtttatatcg taacccacgc ctcccgggga tatgacgcct tcctcggcgt
     2521 cgaacgcagc gacgccccct atcggaaaac catagcccca gtgtatgtcg ggcatggcga
     2581 tagagtattt ctgtatcccc gggagatagg cgacgttcat cacctgctcg agcgccttct
     2641 cctcccttat ggcttcgagc atgctttcag tcgtatagac gtgcccgggg acgagcattc
     2701 cccccgtctt cggaatctcc cagaggtagt cgtgtatctt ccttacttca atcctgtcat
     2761 tcacccgaat cttcccatta tgacgtaatc actgaaaacc gaagcccggt cctcctcccg
     2821 gattatctat ggggcgacaa ggccgtactt cccggatttc tttctataga ctacattgag
     2881 atcacccgta tccttgttcc tgaagacgag aaagaacctg tcctcgtcct ggagcaccat
     2941 cagggcctcc tcggtcgtga gaggcttctc gatagagagc ttctcctcgg atataagccc
     3001 ggcttcgcgg acaggcggtt cttcgttctt gcgcttcgcc ttctcacggg ctatctcctt
     3061 ccgcacctcg atgcgcctgt ccttgagacg gttgagctgc ttgtcgatat cgtcgagtgt
     3121 gaattcgagc gacatgaacg tatcgttcga ttcttcccat gcgtggagcg ccccgccgcc
     3181 cggtgtcttg aggtagaggt ctatgtcgac tctgtgcctc tccctgtgct tctccacggt
     3241 gaaagtaacg accgcctcga cctcgccctc tcccatctct ttcaagtgcc gttccagttt
     3301 cgagaggccg tgctcggtta agctcctcaa gtcttccgaa atctcgattc cctttccaat
     3361 gagctcgatc ttcatatatt cacctcttct atatcgtagt taccgggagc gcaagcacaa
     3421 ccctgcactc tatactccgg ctattcctga atcacgtaaa gccccg
//
LOCUS       DASSJH010000018         8003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_4132, whole genome shotgun sequence.
ACCESSION   DASSJH010000018 DASSJH010000000
VERSION     DASSJH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4132"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFJ67_00320"
     CDS             complement(<1..799)
                     /locus_tag="VFJ67_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7288850.1"
                     /translation="MKAIAVIPGKPGSVHLTDLPKPGVNDVPGGRGVLVKVLRVGVDG
                     TDKEINAAEYGASPSGYDFLVIGHESFGIVEEVGKNVTEFAPGDYVTATVRRPGGSIY
                     DKIGKYDTTTDDTYFEHGINLLHGFLTEYYVDEPEYLVKVPKGLKEYGVLLEPTSVIE
                     KGIAQAYEIQRRLRVWHPKRAAVLGAGTIGLLATMSLRLRGMQVHTFGRTKPPYLNSN
                     LITEMGACYHSTDNINLLEASEKHGPFDIIFEATGFSPIVFEAMNVLG"
     gene            complement(925..1695)
                     /locus_tag="VFJ67_00325"
     CDS             complement(925..1695)
                     /locus_tag="VFJ67_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7288851.1"
                     /translation="MFKDLKDIFYRKESPTPPTEEMQFIEGPHSRLRELWFLFRVMRE
                     FIRGFRILHFVGPVVTVFGSARTKEGSPKYELARNIGYELATMGFAVMTGGGPGIMEA
                     ANRGAKEAGGKSIGCNIKLPMEQKPNPYLDIMVTFKYFFVRKLMLLKYSYAFVVLPGG
                     AGTMDELFETLTLIQTKKIYNFPIILLGRDYFKPLMDFLDLMVIERTLDENDLGFLVF
                     TDSVEEAVSYIQKNIVDKFRIRLKKIPKPSKILLEKKV"
     gene            complement(1793..2959)
                     /locus_tag="VFJ67_00330"
     CDS             complement(1793..2959)
                     /locus_tag="VFJ67_00330"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HET7288852.1"
                     /translation="MTKHLFPLLVCVAVVISGCAAKTAVHDQPPPVEHQFTFSWEFTD
                     SDAMKPRGGVTTGAPVELDKSPNPGWVSVQEPGVSKYERDRRAILSMAGTYRVTFNFI
                     ETIPLRPGYKPDRPYQSWATEYIKVIEDTGNFISLQHILVMYFVDDKGNVEGPSVSKH
                     WRQDWTYEDEEILEYKGNNTWQTVHVPKSEASGKWSQAVYQVDDTPRYEALGEWTYDG
                     NYAAWTSPVTWRPLPRREFSVRSDYNVLSGVNRITITPTGWVHEQDNIKMVVDGNGRE
                     IAKDPYLTKETGLDRYERLSNFNDTAAKEYWGHTAPFWADVRAVWVDVIEANPTLKLN
                     NTYQDRKLYEYLFDYSDKLAEGGVYDSKEGMEYVTGIINSYVVESGKPSDKTVY"
     gene            3260..3916
                     /locus_tag="VFJ67_00335"
     CDS             3260..3916
                     /locus_tag="VFJ67_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288853.1"
                     /translation="MLTLFFIYPGPQASRADVLCANDKSGTVRIRPEECESNETVVDL
                     SGIALSPAVKPPRWRWLGTGEGTYWYVPKAGLTAVEWDTSDPQSYSPVVDQTVWHIDN
                     YANGYFSGTVVAQIGIHPPQCQYLIGSVTPSGAVYITFNSLTDQPVGSPALTTGIGRM
                     VLKGNDWTFNMQMASGPSSSQITHWAYMLECTPDQKCWTDLPGIQQSLPDFLANCSSE
                     "
     gene            4060..4269
                     /locus_tag="VFJ67_00340"
     CDS             4060..4269
                     /locus_tag="VFJ67_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7288854.1"
                     /translation="MAERTIGTVKWFNSTKGFGFIEVENGDDVFVHYSEIKATGFRTL
                     EEGQRVEFTVVKGQKGPQAQNVVLA"
     gene            complement(4326..5162)
                     /locus_tag="VFJ67_00345"
     CDS             complement(4326..5162)
                     /locus_tag="VFJ67_00345"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HET7288855.1"
                     /translation="MINPAQYRLKEKRPISLSILLALIISACVSNFFPAVARAQDVNP
                     NIDPKAASILQDMSYYLGSKYEYTYKAEMMFDDVLESKQKIQYNAEETVYLKKPDKFY
                     VGYVTDIGGYKLWFEAGKATLLEVPVNDYSLATLPSTIDQSLNKLKEEYQFTPALSEF
                     MYIDINKLMTADVISGAYFGISKVMGTNCHHLVFVQKDIDWQIWIEDGKRPVPRKLVI
                     TYKNVAESPQFIALIKDWVTDKPITNFAFKPEIPDVNNRVEFNQITGNQKFKIGSIRA
                     YE"
     gene            5495..6715
                     /locus_tag="VFJ67_00350"
     CDS             5495..6715
                     /locus_tag="VFJ67_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288856.1"
                     /translation="MRSKLAAIAVLFILQIFLVPSSRAGFSFVPNNLMTTPYGPAYAD
                     ILLESTNFLPCQGGPFALCYYSGPEPETCELVGDGTFANCKCFEIAFGQYFVDINAIL
                     DLDIYLETIDVCGADGSGCQTTNSAPVCASINSGNFIPGSDTVSVFSFDCVPEEGIGD
                     TNCPQSLYAGCMTAPCFRTGEEGIVECSCPTYDGPYQVGLDDQACTLGDDLVWSAAYN
                     PNITGTAPVPPPGGCIPDAPESLGGCPLISSDVPPPPPDVDCGEVCEEYSGCVTSNDV
                     EAGYTCDATLCTSRCNDRNLVGDACSGLQGCDISEIVKLEEEVGCSCCASQICGCEAS
                     NKTEHAVYDLNKRQREKDITPQCDINGTLCGEKGGSGSGCALASPSGDSGNPILFLLT
                     GLTVIVMLRRRSRP"
     gene            6741..6941
                     /locus_tag="VFJ67_00355"
     CDS             6741..6941
                     /locus_tag="VFJ67_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288857.1"
                     /translation="MRYLFIVLTVIMLAAAAVYITAKPVMCDDCNQGKKCNTSYDCGE
                     IGVCYCQYTFEEEQGEGTCYFE"
     gene            complement(6980..7684)
                     /locus_tag="VFJ67_00360"
     CDS             complement(6980..7684)
                     /locus_tag="VFJ67_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288858.1"
                     /translation="MKNISPISISFAVLLSICASPQALAQTEGFNVKTPVQTISDGSS
                     EFINGIASDAKADPKTSIPRELLCGAECILALPSVQAASGRDDFEGTGLLGCLRAGSG
                     DFAPPIFFRITNIESFNENGGGLIILVTERKGVKSILGDQLQLNPQNSGTGGIGPQAD
                     LSILKPFIAYVKPAGGGIKAFDASGSTLVYDSGDTFNAYQQDLEPVDIMLFGIDVPPA
                     LRGFKNALEAWRGACK"
     gene            complement(7790..>8003)
                     /locus_tag="VFJ67_00365"
     CDS             complement(7790..>8003)
                     /locus_tag="VFJ67_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017658540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7288859.1"
                     /translation="TLTSEYLNGDYEYKSSVDGKTRVLPRWILAAHMFNHQTHHRGQL
                     TTLIMQMGIDPGVTDIPWLPCLKERA"
BASE COUNT         1774 a         2108 c         2100 g         2021 t
ORIGIN      
        1 tcccgaggac gttcatcgcc tcgaacacta tgggcgagaa gcccgtagcc tcgaagatga
       61 tgtcgaacgg gccgtgcttc tcggaagctt ccaggaggtt gatgttgtcc gtgctgtggt
      121 aacatgcccc catctccgtg atgaggttcg agttcaggta agggggcttg gttctcccga
      181 acgtgtgcac ctgcatgcct ctcagcctga gagacattgt agcgaggagc cctatcgtgc
      241 ccgcccccag cacggcggcc cgcttcgggt gccacacgcg cagacgcctc tgtatctcgt
      301 aagcctgggc tatgcccttt tcgatgacgc ttgtcggctc gaggagcacg ccgtattcct
      361 tgagtccctt cgggacctta accagatatt cgggctcgtc gacatagtat tcagtaagga
      421 acccgtggag gaggttgatg ccgtgctcga aataggtatc gtcggtagtc gtatcgtact
      481 tgccgatctt gtcgtaaatg ctgccgcccg gtcttctcac ggtagccgtt acatagtctc
      541 cgggcgcgaa ctcggtcacg ttctttccga cctcttcgac tatcccgaag ctctcgtgcc
      601 caatgacaag gaaatcgtat cccgagggcg aagcaccgta ctcggcggcg tttatttctt
      661 tatccgtacc gtccacgccg acgcggagca cctttacaag cactcccctc ccgccaggga
      721 catcgtttac gcccggtttc gggagatcgg tgaggtgcac actgcccggc ttgcccggaa
      781 tgactgctat cgctttcatg actcggctct ccttacatct gaagttgcca agttagacga
      841 gtggagggga ggtatggatt taaagcttat ctatgtgtag gagtgatgta gaaagcggcc
      901 gtcagaagca gcgtgccgtg aatatcaaac ctttttttcc agaagtatct tcgaaggctt
      961 cggtatcttt ttcagtctga tcctgaactt gtcaacgata ttcttctgta tgtaagaaac
     1021 ggcttcttcg accgaatctg tgaagacgag gaatccgagg tcgttctcgt ccaatgtccg
     1081 ctcgattacc atcaggtcga gaaaatccat gaggggcttg aagtaatccc ttccgagcag
     1141 gattataggg aagttgtata tcttcttcgt ctgaatcagg gtcagggttt cgaagagctc
     1201 gtccattgtg cccgctccgc cgggcaggac tacgaacgcg taggaatatt taaggagcat
     1261 cagcttcctg acgaagaaat acttgaacgt caccattatg tcgaggtagg ggttgggctt
     1321 ctgctccatc ggcagcttta tgttgcatcc gatggacttc ccgcccgctt ccttcgctcc
     1381 cctgttggcg gcctccatga ttccggggcc gcccccggtc atgacggcga atcccatcgt
     1441 cgcgagctcg tagcctatat ttctcgcgag ctcgtacttt gggctccctt ccttagtcct
     1501 cgccgatccg aatacggtga ccaccgggcc tacgaaatgg agtattctga agccccttat
     1561 gaactccctc ataacgcgga acaggaacca gagctccctc aacctcgaat ggggaccctc
     1621 tatgaactgc atctcctccg taggaggggt agggctttct tttctgtaga aaatgtcttt
     1681 caggtctttg aacaagatct ttatgtctgt gttggattta aaaaacgctc cccgatactc
     1741 agccgcatac tttttaaata actcaagtcg aggggtaatg tcaagagacc gctcagtaaa
     1801 cggttttatc ggagggcttg cccgattcga cgacgtagct gttaattatc ccggttacgt
     1861 attccatgcc ctccttgctg tcgtacacgc cgccctccgc cagtttgtcg gagtagtcga
     1921 aaagatattc gtagagcttt ctgtcctggt aagtgttgtt caacttaagc gtcgggttcg
     1981 cttcgatcac atctacccac acggccctca cgtccgccca gaaaggagcc gtgtgccccc
     2041 agtattcctt ggcggccgta tcgttgaagt tcgaaagcct ttcgtacctg tcgagtccgg
     2101 tctctttcgt cagatagggg tctttcgcta tctccctgcc gttcccgtcc actaccatct
     2161 tgatgttgtc ctgctcgtgt acccagcccg ttggagttat cgtgatgcgg ttcacgcccg
     2221 agagcacgtt gtaatcgctc ctgacgctga attcccgcct cgggagcgga cgccacgtga
     2281 cagggctcgt ccaggccgca taattgccgt cgtatgtcca ttccccgagt gcctcgtacc
     2341 tgggggtgtc gtccacctga tacacggcct gtgaccactt gcccgaagcc tcgctcttgg
     2401 gcacatgaac ggtctgccat gtgttattac ctttgtattc gagtatctcc tcgtcctcgt
     2461 acgtccagtc ctgccgccag tgcttggaga cggaaggccc ttcaacattc cctttatcat
     2521 cgacgaaata catgaccaat atgtgctgga ggcttatgaa gtttcccgtg tcttcgatca
     2581 ctttgatata ttcggtcgcc cacgactgat agggacggtc gggtttgtat cccggacgaa
     2641 gcggtatcgt ttcgatgaaa ttgaatgtga ccctgtaagt tccagccatc gacaaaatcg
     2701 cccgcctgtc gcgctcgtat tttgacacgc ccggctcttg taccgagacc cagcctggat
     2761 tgggggattt gtcgagctcg acgggtgcgc ccgtggtaac gcctcccctg ggcttcatcg
     2821 cgtctgaatc ggtgaactcc cagctgaaag tgaattgatg ctctacaggc ggcggttgat
     2881 catgaacggc ggtcttcgct gcgcagccgc tgattacgac cgcaacgcat acgagcaatg
     2941 ggaatagatg tttggtcatg ggaagtcctt ttctctccgg atatcagcgg tttcgggaca
     3001 acagtctttc attccggtac cgaacgatat acgacggata aaagcttacc taaacccctc
     3061 aaagattcga atcgttatcc gatttttata gaaatgtggg gaagataatt ttaatgacag
     3121 agagtttgtg tcctcctaat tccggcacct gacacggagt ttcaaaaaag cagtacaatt
     3181 cgtattgagt tcaaaatacc gaatctgaac ggaaatggag gaaattatga aaattcgtca
     3241 acatgtaccg gcacttattt tgctgacttt gttcttcatt tatcccgggc cccaggcttc
     3301 gagggccgat gtcctctgcg ccaatgacaa gagcgggacc gtcaggatcc gtccggaaga
     3361 gtgcgaatcc aacgagacgg tagtggattt aagcggcata gcgctgtccc ccgcggtcaa
     3421 acccccgaga tggaggtggc tcggaacagg cgagggcaca tactggtacg tacccaaagc
     3481 gggcctgacc gccgttgagt gggatacctc cgatccccag agctattctc cggtcgttga
     3541 ccagaccgta tggcacattg acaattacgc gaatggttac ttctccggaa ccgtggtcgc
     3601 ccagatcggg atccatcctc cgcagtgcca gtatcttata ggatcggtaa ctccgagcgg
     3661 cgctgtgtat attacattca attctctcac cgatcagccc gtgggctcgc ccgcgctgac
     3721 gacggggatc ggaagaatgg tgcttaaggg aaacgactgg acgttcaaca tgcagatggc
     3781 ttcgggcccc tcctcgagcc agatcacgca ctgggcttac atgctcgaat gcacgcccga
     3841 ccagaagtgc tggacggacc ttcccggaat acagcagagc ctgcctgatt tccttgcgaa
     3901 ctgttcctcg gaatagttgg aattattaat agttgacgcc gggaattaaa taagttaaaa
     3961 taaagttaag gttgcagtag ttcttgtcaa atgtaagatt accatcttaa tgcgtgaacc
     4021 acttgcataa cccaatttaa aataggaggt agcaccctaa tggctgaacg tacaattggc
     4081 actgtaaagt ggttcaattc tacaaaaggc ttcggtttca ttgaggtcga gaacggcgat
     4141 gacgttttcg tacactacag tgaaatcaaa gcgaccgggt tccgcacgct ggaagaaggt
     4201 cagcgcgtgg agttcaccgt tgtaaaggga cagaaaggtc cccaggctca gaacgtagta
     4261 ctggcttaac ggtaaaaaaa aacagtgagc cgggtttccc cggctcactc ccttattctc
     4321 cgtccttatt catacgctct tatacttccg atcttaaact tctggttccc ggttatctga
     4381 ttgaattcca cgcggttgtt tacgtccggt atttcgggtt taaaggcgaa gttcgttatc
     4441 ggcttgtccg taacccagtc ctttatcaac gctatgaact gcggagactc ggcgacgttt
     4501 ttatacgtga tgaccagctt tctcggtacc ggacgcttcc cgtcttcgat ccatatctgc
     4561 cagtctatat ccttctgaac gaatacgagg tgatgacagt tcgtccccat gaccttgctt
     4621 attccgaaat aagcgcccga gatcacatcc gccgtcatga gcttgtttat atctatatac
     4681 atgaactcgc tcagcgccgg cgtaaactga tattcttctt tcagcttgtt taaagactga
     4741 tctattgttg aaggcagcgt ggcgagcgag tagtcgttta caggcacttc gaggagagtg
     4801 gccttgcccg cctcaaacca gagcttgtaa ccgccgatgt ccgtaacata cccgacgtag
     4861 aatttatcgg gcttcttgag atagacggtt tcttccgcgt tgtactggat cttctgtttc
     4921 gactcgagca cgtcgtcgaa catcatttcc gctttatatg tatattcgta tttacttccc
     4981 aagtagtaac tcatatcctg aagtatgctt gcggctttcg ggtcaatgtt ggggtttacg
     5041 tcctgagctc gggctacagc gggaaaaaag tttgagacgc aggctgatat aatgagggcc
     5101 aataatatcg agagcgagat cggccttttc tcttttagtc tgtattgtgc ggggtttatc
     5161 ataatatcct cctttttctt tatactatgc ccgtcccgta attttgtaaa gcccgagggt
     5221 gtcgaggcgg aatttataga ttatcatatt aaacccggtg cttacaataa gtccgatcag
     5281 tacccgtatg cttctcataa tatgaaggct ttatccgacc ggacatgatg actcgccggt
     5341 cgcgatctcc cgtataaatc ccccgtattg acataaaatt gaaagagaca aataattggg
     5401 aaaaccgttt aataactcac gggttaattt tggagacgag agtgcggttt ggaggttggt
     5461 tccgaaaata caatattaca gggagattat agatatgaga tccaagctcg ccgcaatagc
     5521 agtcctgttt atactccaaa ttttcctagt tccgagctcc cgcgccggat tcagcttcgt
     5581 gcctaacaac cttatgacca caccttacgg tccggcttat gccgacatac tgctcgaatc
     5641 aacaaacttc ctgccctgcc agggcggccc gttcgctctc tgctattact cgggcccgga
     5701 gccggaaacc tgcgagctcg tcggggacgg tacattcgct aactgtaaat gcttcgagat
     5761 cgcgttcggg cagtatttcg tcgatataaa cgcgatactc gatctggaca tataccttga
     5821 gacaatcgac gtatgcggtg cggacgggag cggctgtcag actaccaact cggcccccgt
     5881 atgcgcatcc attaacagcg ggaactttat accgggctcg gacaccgtat ccgtgttcag
     5941 cttcgactgc gttcctgaag agggtatagg agacaccaac tgccctcagt ccctgtacgc
     6001 gggatgtatg accgcccctt gtttcaggac gggggaggaa ggaatagtcg aatgttcgtg
     6061 cccgacctac gacgggccgt atcaggtggg actcgatgac caggcctgca cgctgggaga
     6121 cgacctcgta tggtcggccg cttacaaccc caatattacg ggcacagcgc ccgtccctcc
     6181 gccgggaggc tgcattccgg acgcacccga atcgctcgga gggtgtccgc ttatatcctc
     6241 agacgtcccg cctcctccgc ctgacgtgga ctgcggcgag gtttgcgaag agtatagcgg
     6301 ctgcgtcacg tcaaacgacg tagaggcggg ctacacgtgc gacgcgaccc tgtgcacgtc
     6361 tcgctgtaac gacaggaatc tcgtcgggga tgcatgctcg ggcctccagg gctgcgatat
     6421 atccgagatc gtgaagctcg aggaagaggt cgggtgcagc tgctgcgcga gccagatatg
     6481 cgggtgcgag gcgagcaata agaccgagca tgcggtgtac gaccttaaca agaggcagcg
     6541 ggagaaggat atcaccccgc agtgcgacat aaacggaacc ctctgcgggg agaagggcgg
     6601 gagcggctcc ggctgcgcgc tcgcatctcc gagcggagac agcggcaatc cgatcctgtt
     6661 tctgttgacc ggattaacag ttatcgttat gcttcgtaga agatcgcgtc cttgatctgt
     6721 atataacagg gggtgcagga atgagatatc tgtttatcgt cctgacggtg ataatgttag
     6781 ccgcagcggc tgtctacatc acggcgaaac ccgtgatgtg tgatgactgc aaccagggta
     6841 agaaatgcaa tacctcatat gactgcgggg agatcggggt ctgctattgc cagtatacat
     6901 tcgaggaaga gcaaggcgag gggacgtgtt attttgaata aggcttgatt taattttccg
     6961 gaccggcctg aacaggcgtc tatttacacg cgcctctcca cgcctccaga gcgtttttaa
     7021 accccctcag cgccggcggg acgtcgatgc cgaagagcat tatgtcgacc ggctcaaggt
     7081 cctgctgata ggcgttgaaa gtgtccccgc tgtcataaac gagtgtgctg cctgaagcgt
     7141 cgaacgcctt gattccgcct cctgccggtt ttacgtacgc tatgaaaggt ttcaagatgc
     7201 ttaaatccgc ctgaggacct attcctccgg tgccggaatt ctggggattg agctgaagct
     7261 gatcgcccag gatcgacttt actccctttc tttccgtgac gaggattatc agccctccgc
     7321 cgttttcatt gaatgattct atgttcgtaa tacggaagaa tatcggggga gcgaaatctc
     7381 ccgagcccgc gcggaggcag ccgaggagac ccgtgccttc aaagtcgtcc ctgccggatg
     7441 ccgcctgaac ggacggaagg gcgagtatac attcggctcc gcataagagc tcccggggga
     7501 tcgaggtctt ggggtctgct ttcgcgtcag acgcgatccc gtttataaat tcggaagaac
     7561 cgtcggatat ggtctgtacg ggggttttaa cattaaagcc ctcggtctgc gccagcgctt
     7621 gaggggatgc gcaaatcgat aacaacactg caaatgagat tgagatcggg gaaatatttt
     7681 tcatccgtgt gttctccggg gacttaggta atttcaatcc gaacgacgca ttaaattatt
     7741 cgagaaaagt taagaaacgg ttaagggaaa gttaagtgcg tatttgccct caggctcttt
     7801 ccttaagaca cggtagccac ggaatgtcgg taacgcccgg atcgatgccc atctgcatta
     7861 tcagagtcgt cagctgtccc ctgtggtgag tctgatgatt aaacatgtgc gccgcgagta
     7921 tccatctcgg aaggaccctc gtttttccgt ccacgctgct tttatattcg taatcgccgt
     7981 tcaggtactc agacgtcagc gtt
//
LOCUS       DASSJH010000019         9058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_761486, whole genome shotgun sequence.
ACCESSION   DASSJH010000019 DASSJH010000000
VERSION     DASSJH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761486"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFJ67_00370"
     CDS             complement(<1..578)
                     /locus_tag="VFJ67_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7288860.1"
                     /translation="MQLGMIGLGRMGGNMVRRLIKGGHECVVFDRSQNVVEEYAKEKA
                     VGAASLQEFVKKIKKPRAVWMMVPAAAVDETISGIIPYLESGDILIDGGNSYYVDDIR
                     RAKELTAKSIHYVDVGTSGGVWGLERGYCMMIGGEADIVKHLYPVFATLAPGIGDIPR
                     TPGRDKVGGTAEQGYLYCGPNGAGHFVKMVHNG"
     gene            complement(907..1272)
                     /locus_tag="VFJ67_00375"
     CDS             complement(907..1272)
                     /locus_tag="VFJ67_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288861.1"
                     /translation="MQRIRMFAFSLMIAVACAGLFTVRSFAADEGMKATPGSVTGTVI
                     CLIPDYKKGTVTPVIATGPCNTLPPHQHILMTKEGTVYTLQGLQEGLMKVSLMPQREN
                     VTIAGKIEGSNQGWILWVD"
     gene            complement(1387..1602)
                     /locus_tag="VFJ67_00380"
     CDS             complement(1387..1602)
                     /locus_tag="VFJ67_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7288862.1"
                     /translation="MAGRSESNNILKTVTTCRDATDLLLDFINGELKPETNIEFEKHL
                     GICPDCVAFLNTYKKTIELTKSFLKKR"
     gene            complement(1738..2328)
                     /locus_tag="VFJ67_00385"
     CDS             complement(1738..2328)
                     /locus_tag="VFJ67_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7288863.1"
                     /translation="MSDENLVRMIAESGSEEALNEILKRYRERIYRTALKVTDNHNDA
                     EDVVQEVSIRIFKKAHSFRTDSKFSTWLYRLVTNEAISRLRKIRRERTVSLDDYMPRF
                     EDDGHHAERPVIDWSQEVEKKVADKEIHSIIEMAMRQLSPLDRTVVVLSEIEELTNPE
                     IGKILGLSVLAVKGRLHRARLYLRSKLAVQLGYSAA"
     gene            complement(2446..2709)
                     /locus_tag="VFJ67_00390"
     CDS             complement(2446..2709)
                     /locus_tag="VFJ67_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288864.1"
                     /translation="MITKIKHDNENITLKLDGKIEGVYLHELEGICKCHLAERNLDVT
                     LDFSGVTFIDDRGLEMLLKIRDMRLKIVKCSPFIKTLLNGLAD"
     gene            3077..5296
                     /locus_tag="VFJ67_00395"
     CDS             3077..5296
                     /locus_tag="VFJ67_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7288865.1"
                     /translation="MSGNKLLDESNKKVSLSITPDNIESKRSPGAVPTPPEGRAVRKN
                     GDRHIDDLEILNHLAQGTASVVGANFFNSLVQHLASAMRVRYAFIAQCTDSTMTRVRT
                     LSMWLDDRFGENIEFELRGTPCENVIAGNVCVYPDRLQTLFPEDVGLVEWGARSYLGI
                     PIRDAWENVLGHIAVLDDKPMADEPLGINVLQIFASRAGAEIERKRAEDSLRENYMIL
                     AKKNRYETIISNVTRVVHQSLKPEEVFENAVEALSTNIEMVNCAGIYLIEGDEAVLKA
                     ERGYPAKYLEFTSRIKRPKGFTWETLIKGEPNYCPDIDTVEAANVARRAGVKSFLCVP
                     IRSRGEAIGTLDITSFHKHAFDEDELKLLEIVSRQIETAVNNARQAAELSESRLSLQR
                     ALAEVEELKNRLQIENVYLREEISTENNFEEIVGRSDEIKSVLRSVERVAHTGTTVLI
                     HGETGTGKELVARAIHNLSDRKQRTLVKVNCGAISAGLVESELFGHEKGAFTGALQKR
                     VGRFELADGGTIFLDEVGELPPDVQVKLLRVLQEGEFERVGSSETIKVDVRVIAATNR
                     DLEESVKDKSFRSDLYYRLNIFPIHVPPLRERKSDIPLLANFFLTKFSKKLGRAFTGF
                     SQGTLDRILEYHWPGNIRELQNIVERAVVISNPPEIEIDDSVLGKTESFPGNGTEDAM
                     TLEDVERSHIINVLEDSGWVIDGKRGAAAVLNINPNTLRSRMKKLGIKKPDRAGRAL"
     gene            complement(5307..7319)
                     /locus_tag="VFJ67_00400"
     CDS             complement(5307..7319)
                     /locus_tag="VFJ67_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET7288866.1"
                     /translation="MTIEFGRDICYDPRTSERKEWLVTNGIGGFASGTVAGILTRRYH
                     GLLVAALKPPLGRNLLVTKLDETASYEGRNYPLSANRWSGGTVDPQGYINIERFYLDG
                     AIPVWEYACADAILEKRIWMAEGLNTTYIRYELKRASRPLSLDIKAFVNYRDYHSSTH
                     AGGWRMNVEKTGKGVRVLAYGGAVPFYVLSDTAESEAENDWYYGFNLAIEEYRGLDHI
                     EDHLHAARLRRTLAEGQSFTIIASTDESAGTDGDKALSLRKEYERDVLARFLTKQKPT
                     GLEIDSDLNQLALAADQFIVSRPSEQDKNGKSVIAGYHWFGDWGRDTMISLPGLTIAA
                     GRPEIAKSIIRTFSAYVDQGMLPNRFPDAGEKPEYNTVDATLWYFEAVREYFEDTGDK
                     AFLGEIFPVLDGIIDWHNKGTRYNIKVDPEDGLLYAGENGVQLTWMDAKVGDWVVTPR
                     IGKPVEVNALWYNALRTMAKFAGYLGKPAKKYETAADKALSGFSRFWNESTGYCFDVI
                     DGPSGDDPSLRPNQLFTVSLPESALDSDRQEKIVEACALSLLTSHGLRSLSPGHPDYK
                     GRYGGDSRSRDGAYHQGTVWGWLLGHFALAHMKVHGDPEKARGFLMPMLGQLRSHGVG
                     SLSEIFDGDAPMTPRGCIAQAWTVAETIRAFSEIQKRSDKKSKVKA"
     gene            complement(7358..>9058)
                     /locus_tag="VFJ67_00405"
     CDS             complement(7358..>9058)
                     /locus_tag="VFJ67_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HET7288867.1"
                     /translation="EKIFRERISEADEFYRRINPFNTSGDLKSVQRQAFAGMLWTKQF
                     YYYVVEDWLKGDPNNPSPPAERKHGRNRDWAHLYNDDILSMPDKWEYPWFAAWDLAFH
                     TIPIAIIDPEFAKRQLTLLTREWYMHPNGQIPAYEWAFGDVNPPVHAWAAWRVYNIEK
                     KMYGRKDFRFLESVFQKLLLNFTWWVNRKDREGRNVFEGGFLGLDNIGVFDRSAQLPT
                     GGHIEQADGTSWMGMYCLNMLAIALELAMENPSYEDIASKFYEHFLYIAKAMNMISMD
                     RDGLWNEEDGFYYDVLHLPDGKRLPMKVRSMVGLIPLFAIQTLEPEVLEKLKGFNGRL
                     SWFVENRPDLKENVACMLTKGAKERRLLSIVDKDKLKSVLRKMLDENEFLSPYGIRAI
                     SRHHKDNPYRFNVNGNEYRVDYEPAESTSGLFGGNSNWRGPIWFPVNYIIIESLQKFH
                     YYLGDDFKVECPTGSGNYMTLWEAAAEISRRLIKIFIKDGEGRRPVFGGTEKFQTDPA
                     WNEYIPFHEYFHGDNGAGIGASHQTGWTGLVAKLIQQTAEYSQAPAENKKETPRWDHT
                     "
BASE COUNT         1933 a         2434 c         2385 g         2306 t
ORIGIN      
        1 ccgttatgca ccatctttac gaagtgtccc gcgccgttgg gtccgcagta gagatatccc
       61 tgctccgccg tcccgccgac cttgtcacgt ccgggcgtac gcgggatatc gcctatgccg
      121 ggcgccaggg tggcgaaaac cggatagagg tgctttacga tatccgcctc tcccccgatc
      181 atcatgcagt aaccgcgctc gagcccccaa actccgccgc tcgttcccac gtctacatag
      241 tggatggatt ttgcggtgag ttcttttgcg cgcctgatat cgtccacata gtaggagttt
      301 ccgccgtcga tgaggatgtc tcctgattcg aggtagggaa ttatgcccga aatggtttcg
      361 tcgacggccg ccgcggggac catcatccag acggcccggg gcttctttat cttcttcacg
      421 aattcctgca gtgaggccgc gccgaccgcc ttctccttcg cgtattcctc caccacattc
      481 tgagacctgt caaaaaccac gcactcgtgc cctcccttga tgagccgccg caccatgttc
      541 ccgcccattc tccccagacc tatcatacca agctgcataa ttgcctcctt tgtcggtatt
      601 atctgttctg cactagagtt tagtgcacta ctcggtctgt gacaaccggg aatcttttga
      661 aattatttag ccgctgtcag tagtgatact ggataaacct aatgctttcg tgatgtttta
      721 gagccggcga aatcgagcta tccgtattgt ctccgctact cgatttaacg taatagcgat
      781 ttttgactca cgagttttaa tttagagtga taatcagggt taaggcggcg tttaatcgat
      841 acttataatg attgggtttt aagaggggaa atgagcatct cccctctttt tctgaattta
      901 taatatttaa tccacccaga ggatccaacc ttgattgctg ccctctattt ttccggcgat
      961 agtcacattt tctctctggg gcatcaagga gactttcatc aatccttcct gaagaccctg
     1021 gagagtgtaa actgtgcctt cctttgtcat gagaatatgc tgatgcggcg gcagggtgtt
     1081 gcaggggccg gtagctataa cgggtgtaac ggtgcctttc ttgtaatcgg gtataaggca
     1141 tattacggta cctgttacgc tgcccggcgt agccttcatc ccttcatctg cggcaaaaga
     1201 gcggacagtg aaaaggcccg cacaagcgac tgctatcatt aaactaaatg caaacattct
     1261 tattctttgc atctctaata cctccttatg ggattttata cagctatgaa catttatacg
     1321 cagtaaatga aaaatgggat ccattttttt atggatgctt tagatttaat tggaatatgg
     1381 ccatggtcat cttttcttga gaaaggattt tgtgagctcg atcgtttttt tgtatgtatt
     1441 tagaaatgcc acgcagtcgg ggcagatccc gaggtgcttt tcgaactcga tgtttgtctc
     1501 cggtttcaat tccccgttaa tgaagtcgag caggaggtcg gttgcgtccc tgcaggttgt
     1561 taccgtcttt aatatgttgt ttgattccga tcgtccggcc atgttacccc tttttaaacg
     1621 attagacgaa ccgtgaatag agatggatcc ggaatttacc gggacgggtg caaaatagga
     1681 tttatgtaca tttcaacctt gttcctttcg catatctcct cttcgaagga tggttgatca
     1741 agcagccgaa taaccgagct gtaccgccag cttcgagcgg aggtacaatc tggccctgtg
     1801 gagcctccct tttacggcga gcacgctcaa gccgaggatc ttgcctatct ccgggttcgt
     1861 gagctcctcg atttcgctta gaacgacgac tgtcctgtcg agcggagaaa gctgcctcat
     1921 cgccatctcg ataatcgaat gtatctcctt gtctgccacc tttttctcga cttcctgcga
     1981 ccagtcgata accggcctct ccgcatgatg tccgtcgtcc tcgaaccttg gcatatagtc
     2041 gtcgagcgaa acggtccttt ctctccttat ctttctcagc ctggagatcg cctcgttcgt
     2101 aacgagccgg tagagccagg tcgaaaactt ggagtcggtg cggaagctgt gcgccttctt
     2161 gaatattctt atggaaacct cctgcacaac atcctcggcg tcgttatggt tgtcggtcac
     2221 tttgagcgcg gtcctgtata tcctctccct gtaacgtttc agtatctcgt taagcgcttc
     2281 ctcgctgcct gattcggcga tcatacggac gagattctcg tccgacatat tcggtaaatc
     2341 ctcacgtgag atgcgcacat tcagcattcc atcctcaacc ttactcttca tccttaccgt
     2401 attcattttt aaattccccc tggaattgtt tttttccttc agtaattaat ccgcaagccc
     2461 gttgagcaat gtctttataa aaggagagca cttaactatc ttcaaacgca tgtctcttat
     2521 cttgagaagc atttccaagc ctctgtcgtc tataaaggtc acccccgaga aatccagcgt
     2581 gacgtcgaga ttcctttccg cgaggtgaca tttacagatt ccctccaatt catgcaaata
     2641 caccccttct atctttccgt ctagcttcag ggtaatgttt tcgttatcgt gtttgatttt
     2701 ggttatcatc agcatttctt cactcttcct ctgtttaacg gttatcgatt atgaattcga
     2761 tcctctgtat tccacgtatc ctgcatcgtc aagtttcgtg atcgtcgatt tcatatgacc
     2821 catgtaagtg caatcaccgt gccagatgaa tataggcctg ccaaacctga cctaagtgat
     2881 acatttcatt gagaatattg attcaagcgc cggattgtcg tcaaaagcgg cccggagaac
     2941 gctatgaaat atggtggctg tcacgatata tcgtggcggc tgcggccgtt tataaggaat
     3001 tattagtttt tatcgccgta ttgactttaa cctgccccgg aaaaatactt tataaccggg
     3061 aatcattcgg atacatatga gcggaaataa gcttctggac gaatcgaata agaaggtatc
     3121 gctttcgatt actcccgaca atatcgaatc aaaacgttca ccgggcgctg ttccaacgcc
     3181 gcctgagggc cgcgcggtgc gtaaaaacgg tgaccgccat atagacgatc tggagatact
     3241 caaccacctc gcccagggga cggcttcagt ggttggcgcg aatttcttta actctctcgt
     3301 ccagcatctc gcctccgcga tgagggtgcg ctacgctttc atagcccagt gtacggacag
     3361 cacgatgacg agggtgcgca cgctttctat gtggctcgat gaccgtttcg gagaaaatat
     3421 agaattcgag ctcaggggca ctccctgtga aaacgttata gcgggaaacg tatgcgtata
     3481 tccggacaga ctccagacgc tctttcccga agatgtgggc ctggtcgaat ggggcgcccg
     3541 cagctatctc gggataccga tccgggacgc ctgggagaat gtgctcggcc atattgcagt
     3601 gttagacgat aaaccgatgg ccgacgagcc gctgggcata aacgtgcttc agatattcgc
     3661 gtcgcgcgcg ggcgccgaga tagagaggaa gcgcgccgag gactcgctca gggaaaatta
     3721 tatgatcctg gcgaagaaga accgttatga gaccataatc agtaacgtta cgagagtcgt
     3781 ccatcagtcg cttaagccgg aagaggtatt cgagaacgcg gtcgaagcgc tcagcactaa
     3841 tatagaaatg gtcaactgcg cgggaatata tttgatcgag ggggatgaag ccgttctaaa
     3901 agcggagagg ggctatcccg ccaaatacct cgaattcacg agcaggatca agcgtccgaa
     3961 gggttttacc tgggagacgc tgatcaaggg cgagccgaat tattgtcccg atatagatac
     4021 cgtagaggcc gcgaacgtcg ccagaagggc gggtgtcaag agctttctct gcgtgccgat
     4081 aaggagcagg ggagaagcca taggcaccct cgacatcacg tcattccata agcatgcgtt
     4141 cgacgaggac gagcttaagt tacttgaaat cgtatcgaga cagatagaga cggccgtaaa
     4201 caacgcgaga caggcggcag agctcagcga atcgcggtta tcgctgcaaa gggcgctcgc
     4261 ggaagtcgag gagcttaaaa acaggcttca gatagagaac gtgtatctgc gcgaggagat
     4321 aagtacagag aataacttcg aagagattgt cggaaggtcg gatgagataa agagcgtgtt
     4381 gaggagcgtc gagagggtcg ctcacaccgg aacgacggta ttgatacacg gtgagacggg
     4441 cacgggaaag gagctggtcg cgcgggctat tcacaacctg agcgacagga aacagaggac
     4501 tctagttaag gtcaattgcg gtgcgatatc ggccgggctc gtcgagagcg agctcttcgg
     4561 acacgagaaa ggggccttca ccggggcgct ccagaagagg gtggggaggt tcgagctcgc
     4621 agacggcggt acgattttcc tcgacgaagt cggggagctt ccgccggacg tccaggtgaa
     4681 gctcctgaga gtgcttcagg aaggggagtt cgaaagggta gggagcagcg aaacgatcaa
     4741 ggttgacgtg agggtgatag ccgccacgaa ccgggacctg gaagaatcgg taaaggataa
     4801 atccttcagg tccgacctct attacaggct taacatattt cccatacatg ttccgccgct
     4861 cagggaacgg aaatccgata tccccctcct cgccaatttc ttcctgacca aattctcgaa
     4921 gaagctcggg cgcgccttca cgggcttctc gcaaggcacg ctcgacagga tccttgaata
     4981 tcactggccg ggaaatatcc gtgagcttca aaatatcgtg gaaagggcgg tcgtgatctc
     5041 gaatcccccg gagattgaaa tagacgactc ggtgctgggt aaaacggaaa gtttcccggg
     5101 taacgggacc gaggacgcaa tgaccctcga agacgtcgag cgcagtcaca taatcaacgt
     5161 gctcgaggac tcgggatggg ttatagacgg caagagggga gcggccgcgg tgctcaatat
     5221 caaccccaac acgctaaggt cgcgtatgaa aaagctggga atcaaaaagc ccgatagagc
     5281 cggccgggcg ttgtaagcat gggttgctaa gctttgacct tcgatttttt atcgcttctt
     5341 ttttgaatct ccgaaaaagc gcgaatagtt tcagccactg tccatgcctg ggctatacat
     5401 cctctgggcg tcatcggagc gtctccgtcg aatatctcgc tcaggctgcc gacgccgtgc
     5461 gagcgcagct gcccgagcat cggcattaaa aagccccggg ctttctccgg gtcgccgtgg
     5521 accttcatgt gcgcaagcgc aaagtgcccg aggagccagc cccagaccgt cccctgatga
     5581 taggcgccgt ccctcgacct cgaatccccg ccgtagcggc ctttatagtc cgggtggccg
     5641 ggcgagaggc ttctcagccc gtgcgatgtg aggaggctca gggcgcacgc ctcgacgatt
     5701 ttctcctgac ggtcggaatc gagagcgctt tccgggagcg atacggtgaa cagctggttc
     5761 ggcctcaagg acgggtcgtc gcccgacgga ccgtcgatta cgtcgaagca gtagccggtg
     5821 ctttcgttcc agaaacgtga aaagcctgaa agggctttat ccgcggcggt ctcgtatttc
     5881 tttgccggct ttcctagata cccggcgaat ttggccattg tgcgcagcgc gttgtaccat
     5941 agagcgttta cctcgacggg tttacctatg cgcggcgtca cgacccagtc tcccaccttg
     6001 gcgtccatcc atgtgagctg gacgccattc tcgccagcgt aaagaaggcc gtcttccggg
     6061 tcgaccttga tgttatatcg tgtccccttg ttgtgccagt ctattattcc gtcgagaacc
     6121 ggaaaaatct caccgaggaa agccttatcc cccgtgtcct cgaagtattc gcggaccgct
     6181 tcgaagtacc agagcgtcgc atcgactgta ttgtattcgg gcttttcgcc cgcgtcgggg
     6241 aaacggttgg gaagcatccc ctgatcgacg taggccgaga acgtcctgat tatggacttc
     6301 gcgatctcgg gcctgcccgc ggctatggtc agtccgggaa ggcttatcat ggtgtccctt
     6361 ccccaatctc cgaaccagtg gtagcccgct atcacgcttt tcccgttctt atcctgctcc
     6421 gagggtctcg acactatgaa ctgatccgcg gcgagagcga gctggttcag atcggaatct
     6481 atttccaaac cggtcggttt ttgcttggtt aagaatcgcg cgaggacgtc tctctcatac
     6541 tccttcctta atgatagcgc cttgtctccg tccgttccgg ctgattcatc ggtgcttgct
     6601 attatggtga aggattgccc ctccgcgagc gtgcgtctca gccgggccgc gtggaggtgg
     6661 tcctctatat ggtcaagccc cctgtattct tcgatagcca gattaaatcc gtagtaccag
     6721 tcgttctccg cctccgactc cgccgtgtcc gagagtacgt agaaaggcac ggctccgccg
     6781 taggccagga cccgtacgcc ctttcctgtt ttctcgacgt tcattctcca tccgccggcg
     6841 tgtgtagagc tgtgataatc cctgtagttg acgaacgcct ttatatcgag gctcagaggg
     6901 cgtgaggctc ttttcagctc gtatctgatg tatgtcgtgt ttaacccctc cgccatccat
     6961 attcgtttct ccagaatcgc gtcagcgcag gcgtattccc agaccggaat agcgccgtcc
     7021 agatagaatc tctctatatt gatataaccc tgcgggtcga ctgtgccgcc tgaccagcgg
     7081 ttcgcgctta gggggtaatt cctcccctcg taggatgcag tctcgtcgag cttcgtgacg
     7141 agaaggttcc tgccgagcgg gggcttgagc gccgctacca ggagcccgtg gtatctccgt
     7201 gtcaggatgc ccgcaacagt gcctgaagcg aaccctccga tgccgttcgt gacgagccat
     7261 tcctttcttt cagaggtcct agggtcgtag cagatatctc ttccgaactc gatcgtcata
     7321 gtttagcctt cctctcccgg ccgatgatat attcgcctta cgtatggtcc caccgcgggg
     7381 tctctttttt gttctctgcc ggagcctgcg agtattcggc tgtttgctga ataagcttgg
     7441 ccacaaggcc tgtccatccg gtctgatggc ttgcgcctat cccggctccg ttgtccccgt
     7501 ggaaatactc gtggaagggt atgtactcat tccatgccgg gtccgtctgg aacttctccg
     7561 ttccgccgaa aacaggtctt ctcccttcgc cgtcctttat gaatatcttt atcagtctcc
     7621 gcgatatctc cgccgccgcc tcccataacg tcatgtagtt tcccgagcct gtcggacact
     7681 cgaccttgaa gtcgtccccg aggtagtagt ggaatttctg aagggactcg atgatgatgt
     7741 agttgaccgg gaaccatatc gggccgcgcc agttggaatt gccgccgaag aggccgctcg
     7801 tggattcggc gggttcgtaa tcgaccctgt attcgttccc gttcacgtta aacctgtagg
     7861 ggttgtcctt gtggtgcctg gagatcgcgc ggataccgta ggggcttaaa aattcgtttt
     7921 cgtcgagcat cttacgaagg acacttttca gcttgtcttt atccactatc gagaggagcc
     7981 tcctttcctt ggcccccttt gtcagcatgc aggcgacgtt ctctttgaga tcggggcggt
     8041 tttcgacgaa ccacgagagc cttccgttga agcctttcag cttctcgagc acctcgggtt
     8101 cgagcgtctg tatcgcgaac agcgggatca gcccgaccat tgagcggact ttcatcggca
     8161 ggcgctttcc gtcagggagg tggagcacat cgtaataaaa cccgtcttcc tcattccaga
     8221 gcccgtccct gtccatactt atcatgttca tggctttcgc tatgtagaga aagtgctcgt
     8281 aaaacttgct cgcaatgtcc tcgtagctcg ggttttccat tgcgagctcg agcgctatcg
     8341 cgagcatatt gaggcagtac atacccatcc agctagtgcc gtcggcctgc tctatgtgac
     8401 cgcccgtcgg gagctgcgcg ctcctgtcga atacgccgat gttgtcgagt ccgagaaagc
     8461 ctccttcgaa tacgttcctg ccctccctgt cctttctgtt gacccaccag gtgaagttga
     8521 ggaggagctt ctgaaacacg ctctcgagga atcggaagtc cttcctgccg tacatcttct
     8581 tctctatgtt atagaccctc caggcggccc aggcgtgaac cggggggtta acgtcgccga
     8641 acgcccactc gtaggcgggt atctgtccgt tagggtgcat ataccactcc ctcgtgagca
     8701 gtgtgagctg ccgcttcgcg aattcgggat cgattatcgc gatgggtatc gtatggaacg
     8761 cgaggtccca cgctgcgaac cacgggtatt cccatttgtc gggcatcgag agtatgtcgt
     8821 cgttgtagag gtgcgcccag tcgcggttcc tgccgtgctt cctctcggcg ggaggggagg
     8881 ggttgttggg gtcgcccttg agccagtcct ctactacata atagtagaac tgttttgtcc
     8941 agagcattcc cgcgaacgcc tgtctctgaa ccgatttcag atcgccgctc gtgttgaacg
     9001 gattaatacg tctgtagaac tcgtccgctt cagatatgcg ttccctgaaa atcttttc
//
LOCUS       DASSJH010000020         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1410577, whole genome shotgun sequence.
ACCESSION   DASSJH010000020 DASSJH010000000
VERSION     DASSJH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410577"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..1070
                     /locus_tag="VFJ67_00410"
     CDS             420..1070
                     /locus_tag="VFJ67_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7288868.1"
                     /translation="MQNVNLIELIQQGWIATYPLILFSVVMVAILGERMFALSGVASA
                     TRRLTGMVVPPLARGDIGTAISVCNKHRKSLQARIYHSILADSKATDPFEASEKLEEN
                     RYEETQDLKRNIWILGTIGSSAPFIGLFGTVVGIIKSFHSMSIAGTGGFSVVAGGISE
                     ALVATAAGLVVAIIAVIAYNYLQVRVAAINAELRINHAKFIQAYKQGRSNNGDREI"
     gene            1051..1470
                     /locus_tag="VFJ67_00415"
     CDS             1051..1470
                     /locus_tag="VFJ67_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7288869.1"
                     /translation="MAIGKFDDKTEEDIVAEINITPLTDIFLVLLIIFMITSSALIES
                     GANINLPEVSKTETVSRNVVVALTTSGKIFINEKEITKDSFEVELKRALAESNTKVVI
                     LEGDKDALLGDAVKLISQAKEAGAKEIAIAAEKSEAH"
     gene            1487..2224
                     /locus_tag="VFJ67_00420"
     CDS             1487..2224
                     /locus_tag="VFJ67_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7288870.1"
                     /translation="MSSSRLAIFIVLSLILHAAVFSAIVLLKPVEPDEIARPLMVGVT
                     TQYNDPGKGKGSSLDPAPAKKPENKAEKPVEKKVVKKTVREKPAVKSEKKEIISRDAV
                     SVSREPTAVTSAVTPANGSEKESVSPDGRGKGTGLGESGTGLDTSGSGGGTEIGYPNY
                     GVNPKPDYPRIAMRHGYEGLVVLNVFVMESGSVGKVEIRKSSGYDVLDNSALDAVRKW
                     IFIPGKKNGRAIQSWVVVPIRFDLTNG"
BASE COUNT          658 a          600 c          627 g          472 t
ORIGIN      
        1 aatgacgaag tccggccaat gaaagcaaag cgcttgagac agttggtaag cccgatcctg
       61 ggcatcgtaa tgtgacttta cgaaaagtgc cgccttgaca ccaatttatc ctcacgtatc
      121 ttacttatac tgataacgat attcattctc atatcaaaat ctttttaaaa tgaatcttcg
      181 gcatatcgca tttgtgaaga atccgatcca gtatccgcag acaatcgccg gccgtgcgtg
      241 attttggtca gagcgcttac ggacatcacg gtggaaaaac aaccgagcaa tttttcattc
      301 gtaccttttc cttccgcgat tgaggaaaga atcgtttagg atatgattat gtttgtgcag
      361 ttatactttc agcctgaaac cgccgattta aagaaacctg aatctagagg tgatatataa
      421 tgcagaacgt gaatttgata gagctgatac agcagggatg gatagcaacc taccctctca
      481 tcctattttc ggtggttatg gtagctatcc taggcgagag gatgttcgcg ctgagcggcg
      541 tcgcttcggc gaccagaagg ctcacgggaa tggtcgtacc cccgcttgcc cgcggcgaca
      601 tcggcacggc tatttcagtc tgtaacaaac acaggaagag cctgcaggcc aggatatacc
      661 attccatact tgcggacagt aaagcgaccg acccgtttga agcgtccgaa aaactcgaag
      721 aaaacaggta cgaagagacg caggatctca agcggaatat atggatcctc gggaccatag
      781 gcagcagcgc cccgttcata gggctgttcg gaacggtcgt cggcatcatc aaatcgttcc
      841 acagcatgtc catagcgggc acaggcggat tctccgtcgt agccggcggc atctccgaag
      901 cgctcgtcgc aacggcagcg ggcctcgtcg tggccatcat agcggttatc gcctacaatt
      961 acctgcaggt aagagtggcc gctataaacg cagagctaag gataaaccac gcaaaattca
     1021 ttcaggctta taaacagggg agatcgaata atggcgatcg ggaaatttga cgataagacg
     1081 gaagaggaca tcgtagcgga aatcaacatc acgccgctca cggacatctt cctcgttctg
     1141 ctcataatct tcatgatcac gagctcagcg ctcatcgaat cgggcgcgaa cattaacctc
     1201 cccgaggtct cgaagaccga gaccgtatcg aggaacgtcg tcgtcgccct caccacgtcc
     1261 ggaaagatat tcataaacga gaaggagatc acgaaagata gcttcgaggt ggagctgaaa
     1321 agggccctcg ccgaaagcaa caccaaggtc gtcatactcg agggagacaa ggacgctctt
     1381 ctcggcgacg ccgtcaagct gatcagccag gcgaaggagg ccggggctaa agagatagct
     1441 atcgccgccg agaaatcgga agcccactga acccggagaa aattgcatgt cctcttcgcg
     1501 gctcgcaata ttcatcgtac tatcgctcat actgcacgcg gcggtctttt ccgcaattgt
     1561 tttactcaag cctgtagaac cggacgaaat agcccgcccg ctcatggtcg gagtaacgac
     1621 acaatacaac gacccgggaa agggtaaagg gagctctctc gatccggccc ctgctaagaa
     1681 gcctgagaat aaagccgaga agccggtcga gaaaaaggta gtcaagaaaa ccgtaaggga
     1741 aaaacccgca gtaaaatccg agaagaaaga gattataagc cgggatgccg tctccgtcag
     1801 cagagagcca accgcggtaa cgagcgccgt cactccggcg aacggctccg agaaggagag
     1861 cgtttcgccc gatggcaggg gaaagggaac cggtttggga gagagcggca cagggctcga
     1921 tacgagcggc tccggaggag gaaccgaaat cggttacccg aattacggag tgaaccccaa
     1981 gccggattat ccgaggatcg caatgcggca cggttacgaa gggctcgtcg tacttaacgt
     2041 gttcgtaatg gagagcggga gcgtcggcaa ggtcgagatc cgtaaatcgt ccggatacga
     2101 cgtgctcgac aactccgcgc tagacgccgt aaggaaatgg atattcatac ccggaaagaa
     2161 aaacggccga gcgatccaga gctgggtagt cgtgcccata aggttcgatc tcacgaacgg
     2221 gtagcgtaaa cccggtgccc cgcccctaaa taaattccgc ttgatattcc acaaccctta
     2281 catagactat ttttaatgga gcgtgcattc gggaaaatgg atacatatac cgcttcagag
     2341 tataaatacc ttgcagc
//
LOCUS       DASSJH010000021        11625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_221238, whole genome shotgun sequence.
ACCESSION   DASSJH010000021 DASSJH010000000
VERSION     DASSJH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221238"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFJ67_00425"
     CDS             complement(<1..1051)
                     /locus_tag="VFJ67_00425"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET7288871.1"
                     /translation="MKSGSMTLGILGGGQLGRMSALAAARLGIRVHIYTPEKDSPASQ
                     VAVKTFTGGYGDKKKLKKFAESVDVASYEFENIPVDTVRYIREFRPVYPDCRLLEVSQ
                     NRISEKKYLNGIGILTAKWAPVRSAQDIGRAIDMIGGDRFILKTARLGYDGKGQLVHR
                     RRDDPVKSWGKLNSKELILEEAVDFDCEVSVIIARDMRGKSTLYGPVLNEHKHHILSR
                     SVVPAPIPARTSERALETAHLLADSVGLTGVLALEMFVTKDGSILANEIAPRTHNSGH
                     WTIDACACSQFEQHVRTVCGLPMGEPGRHSDAEMINLIGKDVTKIAKWYKTENACVHL
                     YGKSEIKDGRKMGHVT"
     gene            complement(1061..1558)
                     /gene="purE"
                     /locus_tag="VFJ67_00430"
     CDS             complement(1061..1558)
                     /gene="purE"
                     /locus_tag="VFJ67_00430"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020051581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET7288872.1"
                     /translation="MTTSSKNPAVGIIMGSQSDWETMKNAAETLDALKIGYETKIVSA
                     HRTPERLFEYARGARGRGLKVIIAGAGGAAHLPGMTASMTSLPVLGVPVESKALKGLD
                     SLLSIAQMPAGIPVGTLAIGVAGAKNAALLAAAILSTSDEKVADRLDRFRKKQTAGVA
                     VKPRR"
     gene            1902..3485
                     /locus_tag="VFJ67_00435"
     CDS             1902..3485
                     /locus_tag="VFJ67_00435"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET7288873.1"
                     /translation="MESFLSGNLSLVIFFPLIGIPLILLLSYIYKESDAELKIATLAV
                     TLVEFFLSVPLFTSFAGGVPGMQFETKIPWIASLGISYHVGLDGISLFLVLLTTFIMP
                     ITVLSSWHAIHKGMREFLILMLILETALIGTFAALDMILFFVFWEAVLIPMYLIIGIW
                     GTERRIYAAVKFFLYTAFGSALMLIAILYLFFEHLKQFGAPSMDVLDFYKLTIPFDGI
                     LSPQGLAFLAFFLAFAIKVPLFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFVRF
                     LMPFFPQATEKYLPILIAIAIIGIIYGAFVAFAQKDLKKLVAYSSVSHLGMVMLGIFV
                     LNIQGVEGGIYQMINHGLSTGALFLIVGMIYERRHTKKIAEFGGVAKVMPVFAAFFML
                     ATLSSIGLPILNGFIGEFLILLGTFEFNYVYAALGATGIILGAVYMLTAYQKVMFGPL
                     DKPENKALHDINLREILVLLPIAVLMFVMGIFPKPFLSKMEPSVEALLQSKFPNTAVV
                     RKLGDERPREAQSSPVSPE"
     gene            complement(3424..4455)
                     /locus_tag="VFJ67_00440"
     CDS             complement(3424..4455)
                     /locus_tag="VFJ67_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7288874.1"
                     /translation="MNNGLETQEDRQKADELAAVRGEIVREIGKVIVGQEKVVDHLLI
                     SLMSSGHSLFVGVPGLAKTLLVSTLSQVLNLKFNRVQFTPDLMPSDITGSEVLEKDPA
                     SDTRFFRFIHGPIFCNILLADEINRASPKTQAALLQAMQEKKVTVGGETYPIAAPFLV
                     FATQNPIEQEGTYPLPEAELDRFMFQIDVSYPSYDEEVEIVKTTTGTYKPEPAKILDS
                     KKILEYQELVHRVPVPDHVAEYAVRVARATRPEDPGTPESVRQGVSWGVGPRASQYLI
                     LGSKARAVLSGRYTPTTDDVKAIAPAVLKHRVVLNFRAEAEGVKPDDIIREILVKIEP
                     LGDARLRAS"
     gene            complement(4448..6070)
                     /locus_tag="VFJ67_00445"
     CDS             complement(4448..6070)
                     /locus_tag="VFJ67_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7288875.1"
                     /translation="MSRNLSVIKIILLLMLLSSAAFGASESKDDFGSLLQDGEADIRK
                     WDVSGASAKADKALGLAETDEQKHDAYYLKAVTEYYKGDYGAAGEYAGNALRTAGSGK
                     KDEEDGFMEFIQYSVGKKPEFREVKSEHFTIRYAHPEDYIIAEYGKDVLEKSRFEIGL
                     DLEEYPEEPVIVEIYPDLESFTTASTLPPENVEKTGVVGICKFNRIMVLSPRLLPKGY
                     AWSDTLAHEYTHYLIFLKSENTVPVWLHEGIAKFEESRWRESKRNVLSPFYETILAQA
                     FKKNGLVPIEKMHPSLALLDSAREAQLAFAQAGTSVSFLVDKWGNEGLVGLLEAMKAR
                     DDYKAAISDVTGLDFDTFYGSWKKYLEKKNLTEKIPGIKVKGIRISNNEGEKGDGSED
                     LVDIDNGKARGHTRLGDLLGARGRLRSASFEYEKALWFDPGSPLISTRLASALNSSGE
                     TDRALGILDPLLEFYPENVDIHIVLGRIYLERGNLSKAEESYLSAVSINPFDPEVHTA
                     LAAIYEKEGRTKEAERERKVLDILAKKDGQNE"
     gene            complement(6067..9096)
                     /locus_tag="VFJ67_00450"
     CDS             complement(6067..9096)
                     /locus_tag="VFJ67_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HET7288876.1"
                     /translation="MSGADSREKLERLIVGVEKKRKKYLLLRGLSLTALVFLAALAGV
                     SLLSLASGAPLFYAVLKWALVLCSAAAVYRFIYLPLIKNKSNADTLAEIEKSRAGLGE
                     ETLSACELNEAVSAGDYTRGTSESLALAHISNVAAMLESLDLRDLFPLGRLRKYAIPA
                     AGTAAVAVLALIVAAPEWRGFIFSPELTPSSGGPYLRLADIEITLSYPGYTNIPPAAL
                     RDGTGDIEALKGTRVRFEAKPLDRLGSGSLTTDSGASYPVVIKDGKLTAEFPVLGNGS
                     YRITDGGLETRPFKITASEDKKPEIKISSPQGTEIESGPDGRIEIDYDASDDFGLTEF
                     RLTWEGASGKSGKLIGKAKNSPSAYEGKYTLDTGGLDFMGGDTLKLRVEAADNDSVSG
                     PKAGVSNAITVRLKDASKTHRDVMNYAERLMEEMIGVLGDEIEVSGGGKKSFGQDSGD
                     TDAAGTDTDAITKTQGKLTNAIESASATLKKTLESMKEDNRSDYTYFAGLANMDVRID
                     QLLYERRGIFENFASVDIPRAGRLMKREIAEFEEDILFLDSMIKGEKLRDSLLAGKEL
                     MKEYAELSEMMKKLGETGDQALKAEIEKKLAGLEKLMSELARKMSAMSGDIQEGFLNR
                     DAFKSIDMQKQLDEIAKLMKEGNIGKAMQMMAALEQNLQSMIASLESGMQSFGMSSLN
                     QDMSKLNEIIARLDGLEKEETGLKDKTQGLKDSLLKENGSGGDNLRKFIDEEKEKVEK
                     IIKNLSDAKSKVAGNRTEESSPEGTYLLDSLAQKAAELKNWLDSMEMKEAYKNAKNVE
                     ETSKGLNDLGKAGVGNLNSARGEIGNSERLAGEVARDLERLMGSENQAPGSSAMAKRQ
                     DEVKEETGKLSDELEDLRKEMLLSPDIGGELSDAENHMGRASDNLGGNELSKAISNQD
                     EAVKSLREAKEKAESMLQKMQASAQGQGSPVPMVLGQQRMGQGTQGVDTEYVEIPEAA
                     ENEIGREFKEKILKAMKGGSPDGYSELNKKYYDRIIK"
     gene            9196..9624
                     /locus_tag="VFJ67_00455"
     CDS             9196..9624
                     /locus_tag="VFJ67_00455"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET7288877.1"
                     /translation="MGYKKLLTKKMEFSASHRYWNPGWSEEKNREVFGKSAGPHGHGH
                     NYSLEVTVEGEVDPRTGMIINLYDLKTIMKDVLEDFDHKHLNDDNPHFRDLIPTTENI
                     ARVLWDIFEGRLGDEESCRLYRIRLYETPDLYVDYWRERS"
     gene            9630..10052
                     /locus_tag="VFJ67_00460"
     CDS             9630..10052
                     /locus_tag="VFJ67_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET7288878.1"
                     /translation="MTSVTKRYRFSAGHRLYIRGLSDEENLRIFDTCSNPNGHGHDYY
                     LEVRVAGPVDPGNGMVMCPGELDRTVEDTLAELDYKRLDVEVPYFRERQPTGENIAEY
                     LWEKLRGRLRDRLVHLRLSETSSSYFEYFDEEGYPYER"
     gene            10042..10596
                     /gene="folE"
                     /locus_tag="VFJ67_00465"
     CDS             10042..10596
                     /gene="folE"
                     /locus_tag="VFJ67_00465"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7288879.1"
                     /translation="MSVENAVKEILLEIGENPEREGLRDTPDRMASMFKELTAGYRAS
                     PYAIIKEALFNIKYDEMVVVKDIEFYSLCEHHMLPFFGKCSVGYIPNGKIVGLSKIPR
                     IVEVYSRRLQVQERMTAEIADFLMEVLNPLGVAVVAEAQHLCMAMRGIMKADASMLTS
                     SMRGAFKKDERTRAEFLSFIGKHV"
     gene            10673..>11625
                     /gene="hemA"
                     /locus_tag="VFJ67_00470"
     CDS             10673..>11625
                     /gene="hemA"
                     /locus_tag="VFJ67_00470"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET7288880.1"
                     /translation="MQRIIVVGLSHKTAPVEIRERFSFNQEELEDGLSALASKPHIEE
                     CMIVSTCNRVEVYAVSENTDGCVESINEFLSEFHSISADAFSPYLYTSIGHMAVRHFY
                     KVASGIDSMVVGEPQITGQVKEAFQRAQDAAVVGHELTRLMNRAFAVAKRVRNETGVG
                     SSAVSISFVAVELARKVFEDLKGSAVMLLGAGEMAELAAKHLLNYGASRLLIVNRTFE
                     NAVALAGELNGEPVPFEEFEKRLPEAEILICSTGAPDYLIRPDHVRRALERRRNRPVL
                     LVDISVPRNIDPAIGRLDNAFLFDVDDLEAVAEANRAERKR"
BASE COUNT         2158 a         3410 c         3241 g         2816 t
ORIGIN      
        1 tagtcacgtg tcccatcttc cggccgtcct ttatttccga ttttccgtag aggtggacgc
       61 atgcgttctc cgtcttatac catttagcga tcttcgtgac gtctttgcct atgaggttta
      121 tcatctcggc gtccgaatga cggccgggct cgcccattgg cagcccgcac acggtgcgca
      181 cgtgctgctc gaactgggag cacgcgcagg cgtcgatagt ccagtgcccg gagttatgcg
      241 tgcggggcgc gatctcgtta gcgagtatcg agccgtcctt cgtcacgaac atctcgagcg
      301 cgaggactcc tgtaagtccc acggagtcgg cgaggaggtg cgctgtctcg agcgcgcgtt
      361 cagacgtgcg cgcggggatc ggggcgggga cgacgctcct cgaaagaata tggtgtttgt
      421 gctcgttcag caccgggccg tagagggtgc ttttcccgcg catgtcgcgc gcgattatta
      481 ccgatacctc gcagtcgaaa tcgaccgcct cttcgagtat cagctctttt gaattcaatt
      541 tcccccagct ttttacgggg tcgtccctcc gcctgtggac gagctgtccc ttgccgtcat
      601 atccgaggcg cgcagttttc agtatgaacc tgtcgcctcc gatcatgtcg attgcgcgcc
      661 cgatatcttg agcactccgg accggggccc atttcgcggt aaggatgcca atgccgttca
      721 ggtatttctt ttccgatatg cggttctggg agacctcgag caggcggcag tccggataga
      781 cgggccggaa ttcgcggatg taacgcacgg tatcgacggg tatgttctcg aactcgtagg
      841 atgcgacgtc cacgctctcg gcgaactttt tcagtttctt cttatcgccg taccctccgg
      901 tgaacgtctt caccgccact tgcgaagcgg ggctgtcttt ttcgggcgtg tatatgtgga
      961 cccttatgcc gagcctggcc gcggcgagcg cgctcatgcg gcctaactgt ccgccgccca
     1021 gtatgcctag tgtcatagat ccggatttca tcgggttaat tcaacgcctt ggttttaccg
     1081 ctacgccggc ggtctgtttc ttgcggaacc tgtcgagcct gtcggcgacc ttttcgtcag
     1141 aagtcgaaag tatagccgcc gctagaaggg ccgcgttctt cgcccccgca acgcctatcg
     1201 cgagcgtgcc taccggtatg cctgcgggca tctgcgctat tgagaggagg ctgtccaacc
     1261 ccttgagggc tttgctctcg acgggcacgc ccaggacagg cagcgaagtc atgctggccg
     1321 tcatgccggg caggtgcgcc gctccgcccg cgcccgcgat tatgactttg agaccccgcc
     1381 cgcgcgctcc cctcgcgtac tcaaacaggc gctcgggtgt cctgtgcgca gatactattt
     1441 tcgtttcgta tcctatcttg agggcgtcga gcgtctcggc ggcgttcttc atcgtctccc
     1501 agtcggactg ggagcccatt attatgccga ccgctggatt tttcgatgac gtggtcatgt
     1561 ttttaatgtg cctgccgcgg tgatcgcgat ttacgtatcg gtatttacga tccggtccgg
     1621 cttattgtgc cggaaatttc gttatgaccg ccgcttatgg ggttttatta ttatggaaaa
     1681 cgccccgcaa agtcaaatca tgctgttatt ccgtccttgc ggctccggcg gatcgagcag
     1741 tttcaggcac cggaattccc gtccggtaaa gggaatggaa cgcgcgttat cggaggggat
     1801 tctctaaata aagactgatt cttgaaacat cccccgcctc aagtatattt aaagccctgt
     1861 aatcggcgtc tccggcttca taaagaggaa tcaaccataa tatggaatca tttctgagcg
     1921 gaaacctctc cctggtcata tttttccctc tcatcggaat accgctcatc ctgctccttt
     1981 cctatatata taaagagtcg gatgcggagt tgaagatagc caccctcgcg gtgaccctag
     2041 tcgaattctt cctctcggta cctctcttca cctcgttcgc gggcggagtg cccgggatgc
     2101 agttcgagac gaagataccc tggatcgcaa gcctcgggat cagttatcac gtaggcttgg
     2161 acgggatcag cctcttcctc gtactgctca cgaccttcat aatgccgata actgtactga
     2221 gctcgtggca cgcgatccac aaagggatga gggaattcct cattctcatg ctcatcctcg
     2281 agacggcgct catcggcacg ttcgctgcgc tcgacatgat cctcttcttc gtattctggg
     2341 aagcggtgct gataccgatg tacctcataa tcggcatctg gggaaccgag aggaggatat
     2401 acgccgccgt gaagttcttt ctctacacgg cgttcggaag cgccctcatg cttatcgcca
     2461 tactgtatct tttcttcgag catttaaagc agttcggcgc gccttccatg gacgtgctcg
     2521 atttctacaa gctcactata cctttcgacg gtatcctgag cccgcagggc ctggctttcc
     2581 tcgcgttctt cctcgcgttc gcgattaaag tgccgctctt cccgttccac acgtggctcc
     2641 ctgacgcgca cgtcgaggcc cctacggcgg ggagcgtgat acttgcgggc gtgcttctga
     2701 agatgggcac gtacggcttt gtcaggttcc tcatgccgtt cttcccgcag gctacggaaa
     2761 aatatctccc gatactcatc gcgatagcga taataggcat aatatacggg gcgttcgtcg
     2821 cgttcgcgca gaaggatttg aaaaagctgg tcgcttattc gagcgtaagc cacctcggca
     2881 tggtcatgct cggcatattc gtgcttaaca tacagggagt cgagggcgga atataccaga
     2941 tgataaacca cgggctctcg actggggcgc tcttcctcat agtaggcatg atatacgaga
     3001 ggaggcacac gaagaaaata gcagagttcg gaggggtcgc gaaggtgatg ccggtattcg
     3061 cggcgttctt catgctagcg acgctttctt cgatcgggct cccgatatta aacggattta
     3121 taggcgagtt cctgattctg ctgggcacgt tcgagttcaa ctatgtttac gcggcgcttg
     3181 gggcgacggg catcatactc ggcgctgttt acatgctcac ggcttatcag aaggtgatgt
     3241 tcggccctct cgacaagcct gagaacaagg cgctccacga tataaacctg agagagatac
     3301 tcgtccttct accgatcgcg gtgttgatgt tcgtgatggg tatattcccg aagcctttcc
     3361 tctcgaagat ggagccgtct gttgaagcgc tccttcagag caagttcccc aatacagccg
     3421 tcgtcaggaa gctcggagac gagcgtcccc gagaggctca atcttcacca gtatctcccg
     3481 aataatatcg tcgggcttga cgccttcggc ttcggccctg aaattcagaa ccactctgtg
     3541 cttgagcact gcaggcgcta tggctttcac gtcgtcggtc gtcggcgtgt atctgcccga
     3601 cagcaccgcc cgcgccttgc ttccgagtat caggtactgg gaagccctcg gccccacgcc
     3661 ccacgacaca ccctgtctca cggattcagg cgtgcccgga tcttcgggcc tggtggcgcg
     3721 cgccaccctc acggcgtatt cagcaacgtg atcggggacg gggacccgat gaacgagctc
     3781 ctggtattcc agtatcttct tcgagtcgag tatctttgcc ggctcgggct tgtacgtacc
     3841 cgtcgtcgtc ttgactatct cgacctcttc gtcgtaggag gggtaagaga cgtctatctg
     3901 gaacatgaac ctgtcgagct cggcctcggg gagagggtag gtgccctcct gctctatcgg
     3961 gttctgcgtc gcgaagacga ggaaaggggc cgcgatgggg tatgtctctc ccccgaccgt
     4021 gactttcttt tcctgcatcg cctgcagcag cgccgcctga gtcttgggcg aggccctgtt
     4081 tatctcgtcg gcgaggagaa tgttgcagaa tatggggccg tgtatgaatc tgaagaacct
     4141 cgtgtcggat gccgggtctt tctccagcac ttccgagccg gttatgtcgg acggcatgag
     4201 gtcgggcgtg aactgcacgc ggttgaattt gaggttgagc acctgcgaca gcgtgctgac
     4261 gaggagcgtc ttcgccagcc cgggcacgcc cacgaagagc gaatgtcccg aggacatgag
     4321 ggatatgagc agatggtcga ccaccttctc ctggccgact atcaccttgc ctatttcgcg
     4381 cactatctcg cccctcacgg cggcgagctc gtccgccttt tgtctgtctt cctgtgtttc
     4441 gagtccgtta ttcattttgt ccgtcctttt tcgctagtat gtcgagaacc ttcctttccc
     4501 tctccgcctc tttcgtccgt ccttccttct cgtatatcgc cgcgagagcc gtatgtacct
     4561 cggggtcgaa cgggtttatc gatacggccg agaggtaaga ttcctcggcc ttgctcaaat
     4621 tcccgcgctc gagatatatt cttccgagaa cgatgtgaat gtccacgttc tcgggataga
     4681 actcgaggag cgggtcgaga atgccgagcg ccctgtccgt ttcccctgac gagtttaacg
     4741 cggaagcgag gcgtgtggat atgagcggcg agccggggtc gaaccagagc gccttttcgt
     4801 actcaaagga agccgagcgg agcctgcccc gggcgcccag gaggtcgccg agcctcgtat
     4861 ggccgcgcgc cttgccgttg tctatgtcga ccaggtcttc actgccgtcg cccttttccc
     4921 cttcattgtt gcttatcctg atgcccttca ccttgatgcc cggaattttt tccgtcagat
     4981 tttttttctc gaggtatttt ttccacgagc cgtagaacgt atcgaagtcg aggccggtta
     5041 cgtccgagat agcggccttg tagtcgtccc tcgccttcat cgcttcgagc agtcctacga
     5101 gcccttcgtt cccccatttg tcgacgagga agctgacgct cgtacccgcc tgtgcgaaag
     5161 cgagctgcgc ctcacgcgcg ctgtcgagga gcgcgagcga agggtgcatc ttttctatcg
     5221 gtacgaggcc gtttttcttg aacgcctgcg cgagtattgt ctcgtagaac ggactgagca
     5281 cgttgcgctt gctctccctc catctcgact cctcgaactt cgctatgccc tcgtggagcc
     5341 acacgggaac ggtgttctcg ctctttaaga atatgaggta gtgggtgtat tcgtgcgcga
     5401 gtgtgtctga ccaggcgtaa cccttcggaa ggagcctggg cgataatacc attatcctgt
     5461 tgaacttgca tatgccgacg acacccgtct tctccacgtt ctcgggcgga agagtggaag
     5521 ccgtggtgaa gctttcgagg tccgggtata tctcgactat cacgggttct tccggatatt
     5581 cctcgaggtc aagacctatc tcgaacctcg acttctcgag cacgtccttt ccgtactcgg
     5641 ctattatata gtcttccggg tgcgcgtacc ttatcgtgaa atgctcggac ttcacttccc
     5701 tgaactcggg cttcttcccg accgagtact ggatgaactc catgaacccg tcttcctcgt
     5761 ccttctttcc ggagccggcg gtcctgagcg cgttcccggc gtactcgcct gccgctccgt
     5821 agtcgccctt gtagtattcc gtgacggctt tcaaataata agcatcgtgt ttttgctcgt
     5881 ccgtttccgc gagcccgagc gccttgtccg ctttggcgga agctccgctc acgtcccatt
     5941 tccttatgtc ggcctccccg tcctgaagga gactgccgaa atcgtccttc gattccgatg
     6001 ctccgaatgc cgccgacgag agcagcatga ggaggagaat tatttttatg accgagagat
     6061 tccggctcat tttattatcc tgtcgtagta cttcttgttg agctcgctgt aaccgtctgg
     6121 cgagccgcct ttcatcgcct ttagtatctt ctccttgaat tccctgccta tctcgttctc
     6181 ggccgcttcg ggaatctcca cgtattccgt atcgactccc tgcgtcccct ggcccatacg
     6241 ctgctgcccc agcaccatcg gcacggggga gccctgcccc tgcgcgctcg cttgcatctt
     6301 ttgaagcatg ctttcggctt tttccttcgc ctccctgagc gattttacgg cctcgtcctg
     6361 gttcgaaatg gctttggaga gctcgttccc gccgaggttg tcggaggccc tgcccatgtg
     6421 gttctcggcg tccgagagct ctccgccgat gtcggggctg aggagcatct cttttctcag
     6481 gtcttcgagc tcgtccgaaa gttttcctgt ctcctccttg acctcgtcct gtctcttggc
     6541 catggctgag gacccgggcg cctggttctc tgagcccatg agccgctcga ggtcgcgcgc
     6601 gacttctccc gcgagacgct ccgaattccc tatctcaccg cgcgcggaat tgaggttgcc
     6661 gacgcccgct ttccccaggt cgttcagtcc cttggaagtt tcctcgacgt tcttcgcgtt
     6721 cttgtaagcc tctttcatct ccatggaatc gagccagttc ttgagctccg cagccttctg
     6781 cgcgaggctg tcgaggaggt acgtcccctc gggcgagctt tcttccgttc tgttcccggc
     6841 caccttcgac ttcgcgtcgg acaggttctt tataattttc tctacctttt ccttttcctc
     6901 gtctataaat ttgcggaggt tatcgccgcc cgacccgttc tccttgagga gcgagtcctt
     6961 gaggccctgc gttttgtcct tgaggcccgt ctcttccttc tcgagcccgt cgagccgcgc
     7021 tattatctcg ttcagcttcg acatgtcctg gttgagcgag gacattccga acgactgcat
     7081 gccgctctcg agggacgcga tcatgctctg gaggttctgc tcgagtgcgg ccatcatctg
     7141 catcgcctta cctatgtttc cttccttcat gagcttcgct atctcgtcga gctgcttctg
     7201 catgtctatc gacttgaacg cgtcgcggtt gaggaaaccc tcctgtatgt cgccgctcat
     7261 ggcgctcatc ttccgcgcga gctcggacat gagcttctcg agcccggcta gcttcttctc
     7321 tatctcggcc ttgagggcct gatcgcccgt ctcgccgagc ttcttcatca tctccgagag
     7381 ctcggcgtac tccttcatca gctccttccc cgctaggagc gagtccctga gcttttcgcc
     7441 ctttatcatg gagtcgagga acagtatgtc ctcttcgaac tccgctatct cccttttcat
     7501 gagcctgccg gcgcgcggta tgtcaacgct cgcgaaattc tcgaatatac ctctccgctc
     7561 atagaggagc tggtctatcc tgacgtccat gttcgcgagc ccggcgaagt atgtgtaatc
     7621 ggagcggttg tcttccttca tgctctcgag tgttttcttg agcgtcgcgg aggcgctctc
     7681 tatcgcattt gtgagcttgc cctgcgtctt cgttatcgcg tccgtatccg tgccggcggc
     7741 atcagtatcg ccgctatcct gtccgaatga ctttttcccc ccgcccgaga cctcgatctc
     7801 gtcgccgagc acgcctatca tctcctccat gagccgctcc gcgtaattca tcacgtccct
     7861 gtgcgtcttg gatgcgtctt tgagcctcac ggtgatcgcg ttcgagacgc cggccttggg
     7921 cccggagacc gaatcgttat cggccgcttc gacgcggagc ttgagcgtat cgccgcccat
     7981 gaaatcgagc cctcccgtgt cgagcgtgta tttcccttcg tatgcggagg gggagttttt
     8041 cgccttccct atcagcttgc ccgacttgcc ggacgctcct tcccacgtga gcctgaattc
     8101 ggtaaggccg aagtcgtcac tggcgtcgta gtcgatctct atcctgccgt cggggcctga
     8161 ctctatctcc gtgccctggg ggctgcttat cttgatctcg ggtttcttgt cctcgctcgc
     8221 ggttatcttg aacggtcttg tttcgagccc gccgtcagtt atccggtagc tcccgttccc
     8281 gaggacgggg aattcggctg tcagctttcc gtcctttatt acgaccgggt acgaggcccc
     8341 gctgtcggtc gtgagactcc ccgatccgag cctgtcgagc ggcttcgctt cgaaacggac
     8401 cctcgtgccc ttaagcgcct cgatatcccc ggtgccgtcc ctgagggcgg cgggcggtat
     8461 attcgtgtaa cccggatagc tgagcgttat ctctatatcc gcgagcctga gataaggccc
     8521 gcccgaggac ggggttaatt ccgggctgaa aatgaaacct ctccattcgg gggcggcaac
     8581 gataagggcg agcacggcaa cggctgccgt tcccgccgct gggatggcgt actttctcag
     8641 tctgccgagg gggaagagat ccctgaggtc gagggattcg agcatcgccg caacattact
     8701 tatatgcgca agagcgaggg attcggacgt gcccctggta tagtcccctg cgcttacggc
     8761 ttcgttcagc tcgcaggcgc tgagggtttc ctcgccgagc ccggcccggc tcttctcgat
     8821 ctcggccagc gtatcggcgt tcgatttgtt tttgataagt ggcaaataaa tgaatctata
     8881 gacggctgct gctgagcaca acacgagcgc ccacttaagc accgcgtaga ataaaggcgc
     8941 gcccgaggcg agcgaaagga gggacacacc ggcgagagcc gcgaggaata cgagcgctgt
     9001 tagagagagt ccccgtaaga gcaggtattt ctttctcttt ttttcgaccc cgactatgag
     9061 tctctcgagc ttttccctgg aatcggctcc actcatgtat aaaaaaggtt acctcactca
     9121 tccggcttac ggaagttggt accgtcaatt tactatgcta tcctaatcgg tcgccggatg
     9181 ataagaagga tactgatggg ttataagaaa cttctaacga aaaagatgga attttccgct
     9241 tctcaccgtt actggaatcc gggatggagc gaggagaaga accgggaggt gttcggaaag
     9301 tccgcaggcc cccacggaca cggacataac tacagcctgg aggtgaccgt ggagggagag
     9361 gtcgatcccc ggacgggaat gatcattaac ctctacgacc taaaaaccat aatgaaggac
     9421 gtgctcgagg atttcgacca caagcactta aacgacgaca acccgcattt cagagacctg
     9481 ataccgacta ccgagaacat cgcgcgcgtg ctctgggata tattcgaggg aaggttgggg
     9541 gacgaagaaa gctgccggct atacaggata aggctctacg agacgcccga tctctacgtc
     9601 gattactgga gggagcgatc ataaaggaag tgacttcggt aacgaaaaga tacaggtttt
     9661 cagccgggca caggctctac ataagagggc tgtcggacga agaaaatctc aggatattcg
     9721 atacctgctc caaccccaac ggtcacgggc acgattatta cctcgaagtc agggtcgcgg
     9781 ggccggtaga ccccggaaac ggcatggtta tgtgtcccgg tgagctcgac cgcactgttg
     9841 aagacacgct cgccgagctc gattacaaga ggctcgacgt cgaagtgcct tatttccgcg
     9901 agcgccagcc tacgggtgag aacatcgcgg aatacctgtg ggagaaactc aggggcaggc
     9961 tccgcgacag gctcgtccat ctcaggctga gtgagacttc gagcagctat ttcgaatatt
    10021 tcgacgagga gggttacccg tatgagcgtt gaaaatgccg tgaaggagat actactcgag
    10081 atcggtgaaa atcccgaaag ggaagggctc cgggacacgc ccgacagaat ggccagcatg
    10141 ttcaaggagc tgaccgccgg gtacagggct agcccgtacg cgataatcaa agaggcgctc
    10201 ttcaatataa agtacgacga gatggtcgtg gtgaaggata tcgagttcta cagcctctgc
    10261 gagcaccaca tgctgccgtt cttcggaaag tgcagcgtcg ggtacatacc gaacgggaag
    10321 atagtgggcc tgagcaagat accgaggatc gtcgaggtgt attctaggag actccaagtg
    10381 caggagagga tgaccgccga gatagcggac ttcctcatgg aggtccttaa cccgctcggg
    10441 gtcgccgtcg tggcagaggc ccagcacctc tgcatggcca tgagggggat catgaaggcc
    10501 gacgccagca tgctcacgag ctcgatgcgg ggggcattca agaaggacga gaggacgagg
    10561 gccgagttcc tgagctttat cggcaaacac gtatgatccc ctccgggata gaaataatcc
    10621 atacctcgta tataattaag gcctgagaat ctgatataag ggtaatgccg agatgcagcg
    10681 catcattgta gtcggactca gccacaagac cgcccctgta gagataaggg agcggttctc
    10741 gttcaatcag gaagagctcg aggacgggct ttcggccctc gcctcgaagc cgcacatcga
    10801 ggaatgcatg atagtctcca cgtgcaacag ggtggaggtt tacgccgtct ccgaaaacac
    10861 cgacggctgc gtcgagagca taaatgaatt cctgtccgaa ttccattcca tatccgcgga
    10921 cgccttctct ccttaccttt acacttcgat aggacacatg gccgtaaggc atttctacaa
    10981 ggtcgcttcg ggcatcgatt cgatggtcgt gggcgagcct cagatcaccg ggcaggtcaa
    11041 agaggctttc cagcgcgcgc aggacgccgc cgtcgtgggt cacgagttga cacggttgat
    11101 gaaccgcgcc ttcgccgtcg ccaagcgcgt ccgcaacgaa actggcgtcg ggtcgagcgc
    11161 ggtctcgatc agcttcgtcg ccgtcgaatt ggcgcgcaaa gtcttcgagg acctgaaagg
    11221 gagcgcggtg atgctgctgg gcgcgggcga gatggcggaa ctcgccgcca aacacctttt
    11281 aaattacggc gcgagtcgcc tgttgatcgt caaccgcact ttcgagaacg cggtcgcgct
    11341 ggcgggcgaa ttgaacggcg agcccgtgcc cttcgaggaa tttgagaagc gtttgccgga
    11401 ggccgaaatt ctgatctgtt caacgggcgc gccggactac ctgatacgac ccgaccacgt
    11461 ccgccgcgcg ctcgaacggc gccgcaaccg ccccgtgctg ctcgtcgaca tctcggttcc
    11521 gcgcaacatc gatccggcga tcgggcggct cgacaacgcc ttcctcttcg acgtcgacga
    11581 cctggaggcg gtcgccgagg ccaaccgcgc cgaacgcaag cgcga
//
LOCUS       DASSJH010000022         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1411016, whole genome shotgun sequence.
ACCESSION   DASSJH010000022 DASSJH010000000
VERSION     DASSJH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411016"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..518
                     /locus_tag="VFJ67_00475"
     CDS             195..518
                     /locus_tag="VFJ67_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288881.1"
                     /translation="MAEKKYYFILSIPRGEIIEYSTKLSSYISKFFPGKMNPFTLKPL
                     AILDNEAVTKISIECTEQEIRDFAGRFFEGGYELREQENGEVEIVRTGSNSEPSADSS
                     GITKH"
     gene            901..1101
                     /locus_tag="VFJ67_00480"
                     /pseudo
     CDS             901..1101
                     /locus_tag="VFJ67_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
     gene            complement(1247..1534)
                     /locus_tag="VFJ67_00485"
     CDS             complement(1247..1534)
                     /locus_tag="VFJ67_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288882.1"
                     /translation="MPASNGLKKAFLILILILFIAVPVMSAFSDDDKSGGDVENFPLA
                     PNDPDISDVCTLSDCNPAKPCANPDENCYTLNGVFYCCTMAPDPGMEAVGD"
     gene            complement(1608..2459)
                     /locus_tag="VFJ67_00490"
     CDS             complement(1608..2459)
                     /locus_tag="VFJ67_00490"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET7288883.1"
                     /translation="MYIKIRHTTRYKYTESVFLDPQTVRLRPKSDGSQRLISFDIEID
                     PKPDGIVDIVDVAGNDSTCLWFSGKHDHLTITTRSEVETLRANPFDYIITEPSVAKIP
                     VFYPKYYVPSLRPYYTRNTEPEGQVDQFVSDVIEESGVDTMSFLNTLNTRIYEGFEQL
                     VRHEGPPYPAEITIGTGRGSCRDLTVLFMEACRAVGIAARFVSGYREHEMITEERQLH
                     AWAEVYLPGGGWRGYDPSEGLATADQHVTVSAGVIPDEAAPLTGTYRGSGARSIIDYE
                     IEIVSHA"
     gene            complement(2480..3220)
                     /locus_tag="VFJ67_00495"
     CDS             complement(2480..3220)
                     /locus_tag="VFJ67_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HET7288884.1"
                     /translation="MTFCVGMKVRKGLVGIADTLVTTGSESITARKVTIHQYGRHSLF
                     LMTSGLRSVRDKALTYFNEVLDQDDNSFDKLYKAVNAFAAQVRRVADEDKKALEESGL
                     HFDLFSIIGGQLENDTEHKLYMLYPQGNWVEVGQGTPYYLIGESTYGKPIMDRALSYE
                     LNMETALKIGYLAFDATRTSAIDVDFPIDVILYRADTYDIVEHRYERAELIQISEWWQ
                     KRIREAIRELPHDWVEAAFSKLGSDGAK"
     gene            complement(3217..>3630)
                     /locus_tag="VFJ67_00500"
     CDS             complement(3217..>3630)
                     /locus_tag="VFJ67_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HET7288885.1"
                     /translation="VGTPIDNIQWAALLKSASALDMYRRKYEQIIPDSVVHFLMLDRN
                     FPRAILYCVIMAESSLHSITGTPAGTFRNEAERRMGRLHAELTYARMDEIIKEGLHEY
                     LDRFQTELNSVDDSISSTFFAVGSDASRKFGGIEE"
BASE COUNT          734 a         1096 c          950 g          850 t
ORIGIN      
        1 gagagcgaga ttatcttcct ccggggcttg cagtaaccga ggtgtctcct ctggttcgag
       61 aacacgagct tccagtcctc gaggctccat ttctcctgtt ctctcaccca aattctgagt
      121 atcttttcgc gggaagtcat agcggacagc ttattattat accgtgtata atgaatatgg
      181 aagggacaaa ttgcatggcc gaaaaaaaat attattttat cctctccata ccccgcggcg
      241 agatcataga gtactcgacc aagctgagct cctacatctc gaagttcttc ccggggaaga
      301 tgaatccttt cacacttaag cccttggcaa tattggataa tgaagccgtc accaagatct
      361 cgatagagtg cacggaacag gagataaggg actttgccgg gcgctttttc gaaggcgggt
      421 acgagctccg cgagcaggag aacggcgagg tggaaatcgt ccgcacaggc agcaactcgg
      481 agccctccgc cgactcgtcc ggcatcacca aacactaaca cacacacgta gtgtgtatta
      541 tgattcttcc caacagttgt cagccctcac cagctatgta atttttactt aaatgtagga
      601 gcggcttcca gccgcgacac acacgcagtg tgatttatga ttctcccgtg actcatttgc
      661 acaaccggca cattggttag aaccgtcagc ggcgactcgt cgcccgttcg ccctgagcct
      721 gtcgaagggt cgaatgggtg tcattcatga accccttcat cggaaatctc gtagttcaga
      781 tttaaataat cgcggctgga agccgctgct acagtgatgt gaagttttgt acgcattcta
      841 tcacccggaa atccgctata cttctccata gtgaagaaac aatccctcgc gtcacgcttc
      901 atgaacgaag tcaaagtgta caggcacgtg atgacccacc cccggtgccc gcggctcgcc
      961 cgcatctgcc tcggcgcagc cgtcgcctac gccctctcgc cgatcgatat aatccctgat
     1021 ttcatcccgg tgctcggata cctcgacgac gcgatcattc tccctgcact cgtcttcatc
     1081 gcgctccgcg ccctccccga aggactcgtc gacgaggtgc gcgcggagat gaggttggaa
     1141 gcgtccgctc aatgaagcct tatccgtttg ccgaggttac tggctgggcg gaggaggata
     1201 gggagatcat atacgagcgc aggttctgcg acacaaattg tgtgcgtcag tcccccacag
     1261 cctccatccc cggatcggga gccatcgtgc agcagtagaa gacgccgttg agcgtgtagc
     1321 agttctcgtc agggttggcg cagggcttcg ccgggttgca gtcggaaagc gtgcagacgt
     1381 ccgatatgtc cggatcgtta ggcgcgagag ggaaattctc cacgtccccg ccgctcttgt
     1441 cgtcgtctga aaaagcggac attaccggaa ccgcgataaa taaaataagg attagaataa
     1501 ggaaggcttt tttgagtccg ttcgatgcag gcattgtctt tcgctccttt cgggggaatc
     1561 gtctcccccc tattgagaat tctaacgcct gtccccggct aactcaacta tgcatgcgac
     1621 acgatctcta tctcgtagtc gattatcgag cgcgcgcctg agcccctgta cgtgcccgtg
     1681 agcggagcgg cttcatccgg gatgaccccg gccgacaccg ttacatgctg gtccgccgtc
     1741 gcgagccctt ccgaagggtc gtaacccctc cacccgccgc ccggcaggta aacctcggcc
     1801 cacgcgtgga gctgcctctc ctcggttatc atctcgtgct ccctgtaccc gctcacgaaa
     1861 cgcgccgcaa tgcccacggc acggcaggcc tccatgaaca gcaccgtgag gtccctgcac
     1921 gaccccctgc ctgttccgat cgttatctcg gccggatagg ggggcccttc gtgtctcacg
     1981 agctgctcga agccctcata tatgcgggta ttaagcgtgt tgaggaacga catcgtatcg
     2041 actcccgact cttctatcac gtccgagacg aactggtcca cctgcccttc gggctcggta
     2101 ttgcgcgtat aataaggcct cagcgaaggc acgtaatatt tcggatagaa gacgggtatc
     2161 ttcgccacag acggctcggt tatgatgtaa tcgaacgggt tcgccctcag ggtctcgacc
     2221 tcggagcggg tcgtgatggt aaggtggtcg tgcttccccg agaaccagag gcacgtgctg
     2281 tcgttacccg cgacgtccac tatgtcaacg atcccatcgg gcttcgggtc tatctctata
     2341 tcgaaactta tgagcctctg cgacccgtcg ctcttgggcc tgagcctgac cgtctgcgga
     2401 tcgaggaata cgctttccgt gtacttgtac ctggtcgtgt gtcttatctt tatatacatt
     2461 gtcgcactcc cttcgggatt catttagcac cgtcactgcc tagcttggag aaggccgctt
     2521 ccacccaatc gtgagggagc tcccttatcg cctcccttat gcgcttctgc caccattcgg
     2581 agatctgtat cagctccgcc ctttcgtacc tgtgctcgac gatgtcgtaa gtgtcagccc
     2641 tgtagaggat gacgtctatg gggaagtcaa cgtcgatcgc gctcgtcctt gtggcgtcga
     2701 acgcgaggta gccaatcttg agcgcggtct ccatgttgag ctcgtaggag agcgccctgt
     2761 ccattatcgg tttcccgtag gtcgactcgc cgatgaggta atagggcgtt ccctgcccca
     2821 cctcgaccca gttcccctgc gggtagagca tgtagagttt atgttcagta tcgttctcga
     2881 gctggccgcc gatgatcgag aagagatcga aatggagccc gctttcctca agagccttct
     2941 tgtcctcgtc agcaaccctc ctcacctgcg cggcgaacgc gttcaccgcc ttgtagagct
     3001 tgtcgaacga gttgtcgtcc tgatcgagca cctcgttgaa atacgtaagg gccttgtccc
     3061 tcaccgagcg gagccccgag gtcatcagga acagcgagtg cctgccgtac tggtgtatcg
     3121 tcaccttcct cgccgtaatg ctctcagacc ccgtcgtcac gagcgtatcc gcaatgccga
     3181 cgagcccctt tctcaccttc atgcctacgc aaaacgtcac tcttcaatac ctccgaattt
     3241 tctcgacgcg tccgagccga ccgcgaagaa cgtagagctg atcgagtcgt ccacgctgtt
     3301 gagctcggtc tggaacctgt ccagatactc gtggagcccc tcctttatta tctcgtccat
     3361 cctcgcgtac gtgagctcgg cgtggagcct gcccatgcgc cgctcggcct cgttcctgaa
     3421 cgtgcccgcc ggggtgcccg ttatcgaatg gagcgacgac tcggccatta tcacgcagta
     3481 cagtatcgcg cgagggaagt tcctgtcgag catgaggaaa tgcacaaccg aatcgggaat
     3541 tatctgctcg tatttcctcc tgtacatgtc gagcgcgctc gccgacttca gcagcgccgc
     3601 ccattgtatg ttgtctatcg gcgtgccgac
//
LOCUS       DASSJH010000023         7509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_762247, whole genome shotgun sequence.
ACCESSION   DASSJH010000023 DASSJH010000000
VERSION     DASSJH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762247"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..931)
                     /locus_tag="VFJ67_00505"
     CDS             complement(206..931)
                     /locus_tag="VFJ67_00505"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HET7288886.1"
                     /translation="MIDIHCHLLPNIDDGPKSWEESLEMIRIAYGDGIKGAVTTPHWI
                     QGTNWQPSPDTVRDLVGEFNDRIKKEGIEFTVYPGMEIGIIPDLPRIVSNGEVLTLAG
                     SDFILIEVPFYNLPLGIEEVIFGLEAMGKTPVLAHPERNREFQGSPRRITDLVSMGAL
                     VQVTASSLSGGFGDAAKDCAIEFAKMGVLHFVSSDAHSRKQRAPVVSQGLAVIGKKIG
                     KDAVNDLVSNTYQIINGSSRLRE"
     gene            1052..2515
                     /gene="rfaE1"
                     /locus_tag="VFJ67_00510"
     CDS             1052..2515
                     /gene="rfaE1"
                     /locus_tag="VFJ67_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET7288887.1"
                     /translation="MRVDETLKRFGRQKILVVGDIMLDRYVRGAVERISPEAPVPVLR
                     VEGEESAAGGAGNVAVNLRTLGAKVELVGSVGKDREGDEVLRLLGDNGIGTSGVVRDG
                     VKHTTTKTRLIAGNQQIARIDSETVSGKAGKSEKALLKSIERALARFKPKGIIISDYS
                     KGVLSNNLISHIIRLAKKSGVFVTVDPKGDNFARYRGADAITPNLKEAEHASGIKIAD
                     ERTLGKAAGKIMKETEADFVIITRGAAGISYFSRSGESGTVPSHPVEVFDVTGAGDTA
                     ASVFTLSYLASRKLEESVKIANAAAGIVVSRIGTSSVTKSDLEAHFAREEEKEKSKIR
                     TRGTLAKTLASLRAKGRSVVFTNGCFDLFHTGHLKLLREAGKLGDALVVAINSDGSVR
                     RLKGPDRPYISEADRALLIAALDCVDYICVFEEDTPLKLIKELKPDVLVKGGDYSRED
                     IVGGDFVESTGGRVAIVPLIKGISTSQLAEKIKNSKK"
     gene            complement(2584..3351)
                     /locus_tag="VFJ67_00515"
     CDS             complement(2584..3351)
                     /locus_tag="VFJ67_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7288888.1"
                     /translation="MKRISLVCLFITLVLLSCGGGKKKADVEVDQSKNFENQKSLAAA
                     SSWRGNFPQALKEIEEAEKINDKDPDVYVIKGAIYMGLKEYPLAEQNYKKALTLSPDY
                     TPAHFNLCGLYLIQKNFDQVISECGLVVADPTYKARANAYTNIGIAYFNKGDMAKARE
                     NYEQALKLNPSFVYAHNEMGELYMSSGRYGEAVNEFQLAVKGYPTYDEAYYNLGLAYL
                     KLNDNVNACEAFRKVMEISPDSESGVNTNRYINTVCK"
     regulatory      3576..3660
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4000..>7509
                     /locus_tag="VFJ67_00520"
     CDS             4000..>7509
                     /locus_tag="VFJ67_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288889.1"
                     /translation="MNTILGKVVLKETGLGIPDLLVVIFDIDPNTQPEEARASGVSIA
                     VPSVIPQADRLGSVLTERNPAQPERNGSFSLTYEDEEFRIRNADEKRPDLLLMVCAPE
                     EPGQDPSAQVLFTSPVLRQNAGRTESYLIKIPSKKLEEAGVPVPIAPSLRSEEPNALI
                     HNVTQVVTQRSMIEKEIQKIAGKQVTAARQKSQKIEAEMESRLIEKLTGVPGSMAERL
                     NFVPPGGDVEKVAFNALNKSIEKNFNTQPPVAGYIVLPDADVERFKDANGNFRTDIAP
                     GEIEPYIFGGANDSERPMSLLREDPVALLCETLSANPDPLGDAGDGGTHTNGHTQPGD
                     TDGDGNGSNGHATPEDISRYIGNLMNGMTPPEELITLGPNKRPTNDDVHNDIQDFTLH
                     TGPADVPALYDFHNLQIAFDYVWQQAIDEGVIQTGTELSRQLIEMGGDPLGAMQNGHD
                     PVKVLRSELKNVQVAHAGLNASGINFSQPGVVYMRKNDTDGIPDPPPGPPRPGWPDIV
                     GPFPEVAVLEPLRPRQPHELLDLLEELLGQRYKFETYAPGSINFGILVTYREMWKLLN
                     HQVGDLVKTITLSPKETRKVSTKRTVKKERSVKEMENNLRVRKDESSDTMRDEAEIVR
                     KAVGKTNFTLTADGSYNIGISKGDSKSTFGKDAETTSQEVKKSFREAVMKAALEYRDE
                     HNITVDTKESFDEETTESAEITNPNDELTVTYLFYELQRRFKISEHIHRLTPVVLVGM
                     EVPNPGRKAVDQILLTHSWIINRVLLDDRYRAPLDYLCTRIVGDELALREMYQTVSKL
                     REIVDEMKKKLSVIVGQVEVRNSTLEKALEKRAALTASESTEGMGEKVWEWAVGSGDD
                     EAIEAARLREEAAREAYDKAVRERDALLDELRSESAALNAATESYAKAYAEHSNHLLE
                     IAGLRVHFKENMLFYMQAIWSFTFQDQIFFMLHKLKAPRLSSTVKTYSVAPAAVIPAS
                     IVVKPGHTVLEVHATVKLKSGLDPAEDFVTLAEIADLDSPLGYKGNYMIFPLKQSNSL
                     TDFMMMPYVDSELGLHDPDELGNWTPEDFTKYVACLRANLPAGEFDEVKDQLREQYMR
                     ILSAPRRAEDEVVVPTGSLFIEALPGAHPILEDFKLLHRVVDVKKVHAEVRRLEMENI
                     RYAARILSGEREDPEIEKKITVEG"
BASE COUNT         1813 a         2087 c         2144 g         1465 t
ORIGIN      
        1 agtctagatt gaggagcgtc aacgtcccgg ccgcgaaaca cacgatcgaa atactggaaa
       61 taatcaagga caagaccgcg ggcaacctcg acgaagacga agacaagctc ctggacgaca
      121 tcctttataa ccttagactg aagttcctcc agcattcgaa ggtggaataa aataagcgtt
      181 tatattctga aaccggcggg ggctgtcact ctctcaaacg cgagctgccg ttgattatct
      241 ggtaggtgtt cgacaccaga tcgttgaccg catccttccc tattttcttt cctattactg
      301 ctaacccctg tgacaccacg ggcgctcttt gttttctcga atgcgcgtcg gaggaaacga
      361 aatggagcac gcccattttg gcgaattcga tagcgcaatc cttagccgca tcgccgaaac
      421 ctcccgagag gctcgacgcc gtgacctgca cgagggcgcc catgcttacc aagtcggtga
      481 tcctcctggg cgagccctgg aactcgcggt tccgctccgg gtgggccagg accggggtct
      541 tgcccatggc ctcgagtccg aaaatcacct cttcgatccc gagcggcagg ttatagaacg
      601 gtacctctat aagtatgaag tcactcccgg cgagtgtgag cacttccccg ttcgacacga
      661 tccgcggcag gtcggggatt atcccgatct ccatgccggg ataaacagtg aactcgatcc
      721 cctctttttt tatcctgtcg ttgaattccc ctacaaggtc tctcacagta tcggggctgg
      781 gctgccagtt cgtcccctgt atccagtgcg gagtcgtgac ggcccccttt attccgtccc
      841 cgtacgcgat ccttatcatt tccaggctct cttcccagct cttcggtccg tcgtcgatat
      901 taggcagcag gtggcagtgt atgtcgatca tcccgtagaa gtatatacga cgaaaacggc
      961 ccgtaaaccg ctttaattta cagtattttc gatcttcatt ataattagag cggttccggg
     1021 cggatttatt ccggtacgga aaaggaaaat catgcgcgta gatgagacac tcaaacgttt
     1081 cggcaggcag aagatactcg tcgtcggaga catcatgctc gaccgctacg tgaggggcgc
     1141 cgtggaaagg atatcgcccg aggcccccgt gcccgttctc agggtcgagg gggaagaatc
     1201 cgcagccgga ggcgccggga acgtagccgt gaacctgaga acgctcggcg cgaaagtcga
     1261 gctcgtcggc agcgtgggaa aggacaggga aggagacgag gtactgaggc ttctcggaga
     1321 caacggcatc gggacgagcg gagtcgtccg cgacggggtc aaacacacga ccactaaaac
     1381 gagactcatc gccgggaacc agcagattgc gcgcatcgac agtgagaccg tctcggggaa
     1441 agccggtaag tcggagaaag cgctccttaa atcaatagag cgcgctttag ccaggttcaa
     1501 acccaaagga ataataatct cggattactc aaagggtgtt ctcagcaata atctgatctc
     1561 gcatatcatc aggctcgcaa aaaagtccgg cgtcttcgtc acggtcgacc cgaaagggga
     1621 caacttcgcg aggtacagag gggcagacgc catcacgccc aatctcaagg aagcggagca
     1681 cgcctccggc ataaaaatcg cggacgagag aacgctcggt aaagcggccg ggaagataat
     1741 gaaggaaaca gaagcggatt tcgtcatcat cacgcggggc gcagcgggca taagctattt
     1801 ctcgaggagc ggcgaatccg ggacggtgcc ttctcacccg gtcgaggtat tcgacgtgac
     1861 gggcgcgggg gatacggcgg cgagcgtatt cacgctctca tatctcgcat cccggaagct
     1921 cgaggaatcg gtaaagatag caaacgccgc agcggggata gtcgtaagcc gcatcgggac
     1981 ctccagcgtg actaaatccg atctcgaagc ccacttcgcg cgggaggagg agaaggaaaa
     2041 gagcaagata cggacgcgcg ggacgctcgc gaaaacgctc gcctcactca gggccaaagg
     2101 gcggagcgtc gtattcacga acgggtgctt cgacctcttt cacacaggac atctcaaact
     2161 gctgagggag gccgggaagc tcggggatgc gctcgtcgtg gcgataaaca gcgacgggtc
     2221 cgtgaggagg ctcaagggac cggacaggcc ctacataagc gaggccgacc gggcgctgct
     2281 catcgcggct ctcgactgcg tcgattacat ctgcgtgttc gaagaagaca ctccgctcaa
     2341 gctcataaag gagctaaagc cggacgttct cgtaaagggc ggagattaca gccgggaaga
     2401 tatagtcggc ggggacttcg tcgagagcac aggcggacgt gtggcgatag tgcctctcat
     2461 aaaaggaatc tcaacgtctc agcttgccga gaagataaag aacagtaaga aataaccgcc
     2521 gctatcatca ttacccgcac ggcgtaataa tccggtatcg gagacgaccc gtctttcctg
     2581 cagttactta catacagtgt ttatgtaccg gtttgtgttc acgccggatt cggagtcggg
     2641 agagatttcc atgacttttc tgaacgcctc gcaggcgttc acgttgtcgt tcagtttcag
     2701 atatgcgagt cccaggttat agtaagcctc gtcgtacgta gggtaaccct tcaccgcgag
     2761 ctggaactcg ttaaccgctt ccccgtacct gcctgaggac atgtagagct cgcccatttc
     2821 gttgtgcgcg tatacgaagg acgggttcag cttgagggcc tgctcgtagt tctccctcgc
     2881 cttggccata tctcccttgt tgaaataagc gatgccgatg ttggtgtacg cgttggctct
     2941 cgccttgtac gtcgggtcgg ccacgacgag gccgcattcg gaaatgacct ggtcgaaatt
     3001 cttttgtatg agatatagtc cgcagaggtt gaaatgcgcg ggcgtatagt cgggactgag
     3061 agtgagcgcc ttcttatagt tctgctcggc gagcgggtat tccttgagcc ccatgtatat
     3121 agcgcccttt atgacataga cgtccgggtc cttgtcgtta atcttctcgg cctcctcgat
     3181 ctccttaagc gcctgaggga agttgcccct ccacgatgac gccgcggcta gtgacttctg
     3241 gttctcgaag ttcttcgact ggtcgacttc gacgtcggct ttcttcttcc ctcccccgca
     3301 ggagaggagc actagagtga tgaaaagaca gactagggat atacgtttca tttagccgta
     3361 gttaacctac acaattgcga aatataatat cacaagatta caagcactta caaggtagga
     3421 ttttatatcg tcctatcccc cggcgcggca gccgtccatg ccttcatctt gccgcctccg
     3481 gaaccgccgc cgtaacactt gtattaccgc agacaaaatt tcaaagatcg gaatttccga
     3541 ttgacactgt catgtagttt gtattagaat cgtaattgaa aaaagggcaa accatttgaa
     3601 agaatgggac gcaaagtcac aggcctaaga acttccgttt acggcggctg gattgcgatt
     3661 tcaacctacc tgacttccgt acttgggctc cggcttttaa atcccatatc gataattgaa
     3721 ccctttttat tccgaaacgg ttttagaggg tgattggaat ataaccgttc cgaaaaacac
     3781 aggagagctg tggtatccga aggggataac agggccgacc gacctctcaa cagaacaaac
     3841 ttttaattcg atatagcgcc gcgtcatttt cggggccata gcccccggtt cggctcagtc
     3901 atgcagtgtg taaagctgta agcagcacgg gtctctgcct tccagattat ttacgtaatg
     3961 acttttcata aaccttaaaa aaattacgcg gaggtaatca tgaatacaat tctcggaaag
     4021 gtcgtgctta aagaaacagg tctcgggata cccgacctgc tggtcgttat tttcgacatc
     4081 gatcctaaca cgcagcctga agaggcgcgc gcgtcgggtg tttccatagc ggtaccgagc
     4141 gtgatccctc aggcagaccg tctcggctcc gtgctgacag agcgcaaccc ggcgcagccg
     4201 gagcgcaacg gttctttctc gcttacctac gaggacgagg agttccgtat ccgtaacgcg
     4261 gacgagaagc gccccgacct cctccttatg gtttgcgctc ctgaggagcc cggacaagac
     4321 cctagcgcgc aagtcctctt cacctcaccg gttttgcggc agaatgcggg gcgtacggaa
     4381 tcctatctga taaaaatacc ctccaaaaag cttgaagagg cgggcgttcc ggtcccgata
     4441 gctccctcac tgaggtcgga ggagccgaac gcgctcatac acaatgtgac ccaggtagtc
     4501 acacagcggt cgatgattga aaaggaaata cagaaaatag ccggcaagca ggtcacggca
     4561 gcccggcaga agagtcagaa gatcgaagcc gaaatggaaa gccgcctcat cgagaagctg
     4621 accggcgtgc cgggtagcat ggcggagcgt ctcaacttcg tgcctcccgg cggagacgtt
     4681 gagaaggtcg ccttcaacgc gctcaataag agcatcgaaa aaaacttcaa cacacagccg
     4741 cccgtagcgg gatacatagt gctgccggat gccgatgtcg agcgcttcaa ggatgcgaac
     4801 ggcaacttcc gtacggacat cgcccccggc gagatagaac cgtacatatt cggcggcgcc
     4861 aatgactccg agcggcccat gtccctcctc cgtgaagacc cggtcgcgct cctatgcgag
     4921 acgctgtcgg cgaatccgga ccccctgggg gatgccggcg acggcgggac ccatacgaac
     4981 ggccatacac agccgggaga caccgatggt gacggaaacg gctcgaacgg ccacgccact
     5041 cctgaggaca tatccagata tatcggcaat ctgatgaacg gcatgacgcc ccccgaagaa
     5101 ctcatcactc tgggtccgaa taaacggccc acgaatgacg atgtgcacaa cgacatacag
     5161 gactttaccc ttcacactgg acccgcagac gttccggcgt tatatgactt ccacaatctt
     5221 caaatcgcct tcgattacgt ctggcagcaa gccatagacg aaggcgtgat ccagaccggg
     5281 accgagctgt ccaggcagct tatagagatg ggcggcgacc cgcttggcgc gatgcagaac
     5341 gggcacgacc cggtaaaagt gctgagaagc gagttaaaga acgtgcaggt cgctcatgcg
     5401 ggcctcaacg cctcagggat taacttcagt cagccgggcg tcgtatacat gcggaagaat
     5461 gacaccgacg gaatcccgga tccccctccg ggacctcccc gtcccgggtg gcctgacatt
     5521 gtcggcccgt tcccggaagt agccgtattg gagccgctgc ggcccagaca gcctcacgag
     5581 cttctggacc tgctcgaaga gctgctcggg cagaggtaca agttcgagac ctatgccccc
     5641 ggcagtatca atttcggcat cctggtcacg tacagggaga tgtggaaatt gctgaatcat
     5701 caggtcggag accttgtcaa gacgatcact ctttccccca aggaaactcg gaaggtcagc
     5761 acgaagcgca cggtcaaaaa agaacggtcg gtcaaggaga tggagaataa cctccgcgtc
     5821 cgcaaggacg aatcgagcga taccatgcgg gacgaggccg agatcgtgcg gaaggccgtg
     5881 ggaaagacga acttcacact gaccgccgac gggagctaca acataggtat ctccaagggc
     5941 gacagtaaat cgacgttcgg caaggacgcc gagacgactt cgcaggaagt caaaaagtct
     6001 ttccgggaag cggtgatgaa ggcggctctc gaatacaggg acgagcataa tatcactgtg
     6061 gacacgaaag agagcttcga cgaggaaacg accgagtcgg ccgagatcac gaatccgaac
     6121 gacgagctca cggtgactta tcttttctat gagctccaga ggcgcttcaa gatttcagag
     6181 cacatccacc gcctcactcc tgtagtgctc gtcgggatgg aagtccccaa ccccgggaga
     6241 aaagccgtcg accagatact gctcacccac agctggatca tcaacagggt gctgctcgac
     6301 gacaggtaca gggcgccgct cgattacctc tgcacccgca tcgtagggga cgagctcgcg
     6361 ctcagggaga tgtaccagac ggtctccaag ctgcgcgaga tcgtcgatga gatgaagaag
     6421 aagctctcgg taatcgtcgg gcaggtcgag gtgcggaact cgaccttgga gaaggctctc
     6481 gaaaagaggg ccgcgctcac ggcttcggag tcgacagagg gtatgggaga aaaggtatgg
     6541 gagtgggccg taggcagcgg cgacgacgaa gcgatagagg ccgcgcggct gcgcgaggag
     6601 gcggccaggg aagcgtacga taaggctgtg agggagcgcg acgcgctgct cgacgagctg
     6661 aggagcgagt cggcggctct caacgcggct accgaaagct acgcgaaggc ctacgccgag
     6721 cacagcaacc acctgctcga gatagcggga ctccgcgtgc acttcaagga gaacatgctc
     6781 ttttacatgc aggctatctg gagcttcacg ttccaggacc agatattctt catgctgcac
     6841 aaactaaagg ctccgaggct cagcagcacg gtgaagacct acagcgtcgc gcccgctgcg
     6901 gtgattccgg cctcgatcgt cgtcaagccg ggccacacgg tgctcgaggt ccacgcgacc
     6961 gtgaagctga aatcgggcct agacccggct gaggactttg ttacgctcgc cgaaatagcg
     7021 gacctggaca gcccgctcgg ttataaaggc aactacatga tcttcccgct caagcagtcg
     7081 aactcgctga ccgatttcat gatgatgcct tacgtggact cggagctcgg cctccacgac
     7141 ccggacgagc tcggcaactg gacccctgaa gacttcacga aatacgtagc ctgcctgaga
     7201 gcgaacctgc cggccgggga gtttgacgaa gtgaaagacc agctgcgcga gcagtacatg
     7261 cgcatcctga gcgcgccgcg ccgcgccgag gacgaggtcg tcgttcccac gggctcacta
     7321 ttcatcgaag ccctgcccgg ggcgcacccg atcctcgagg atttcaagct gctccaccgc
     7381 gtggtggacg tgaagaaggt ccatgcagaa gtgcgcaggc tggagatgga aaatatccgc
     7441 tacgccgccc gtatcctctc gggcgagcgc gaagacccgg agatcgagaa gaagatcacg
     7501 gtcgaagga
//
LOCUS       DASSJH010000024         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_978328, whole genome shotgun sequence.
ACCESSION   DASSJH010000024 DASSJH010000000
VERSION     DASSJH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978328"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFJ67_00525"
     CDS             <1..488
                     /locus_tag="VFJ67_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HET7288890.1"
                     /translation="YIFAGQWIHVPALELFISTVEIIILPVVLGAGLRLLLGKRIDHV
                     LPVLPGVSSLSIIFIVGVIVAANAASIAKVGAGVAVIVMLHNAAGLVLGYSAARLSGM
                     DVTKARAVSIEVGMQNSGLAVALANLHFSPLAALPAAIFSVWHNISGSAIAWWWRRRT
                     V"
     gene            505..1431
                     /locus_tag="VFJ67_00530"
     CDS             505..1431
                     /locus_tag="VFJ67_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7288891.1"
                     /translation="MTATTTITKRKDRFPIAGYLYLVGTALFTALSYVFGRAIDRSFH
                     PAAITFYWFFGAFVCSVLVAAVIPSQRAEVRHLGKYTKIFIYSSVLTAIGAALWISAI
                     KTIGIPLTSFLMKAQTLFSLLLGMIFLGERLNKWETFGIVVTITGGVIVAYQRELSLL
                     IGTLTALGAAFFYSSLSFTVKKMGQNLNMLTVANLRALGVSICLILYLAATGTLQTPG
                     PVDLAFMALGGITGAYIAKACQFQAIKLIDVSRTTAVLPLESLFVVIFSYYIFHEIPS
                     LIKLIGGAGIIIGVIFLVVFRAEKPEDLGEGE"
     gene            complement(1549..2178)
                     /locus_tag="VFJ67_00535"
     CDS             complement(1549..2178)
                     /locus_tag="VFJ67_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HET7288892.1"
                     /translation="MDLTFFIKGIIVGIVITAPIGPVGALVVQRTINEGRTAGIISGF
                     GSAAGDAVYAAIVAFGLTFISEMLMQREAWVHMIGGIILFVFGVRVYFSKPTPYSENK
                     AARSHFGLFGSAFLLTLSNPMVILSILALFAIIGIHNPANYYRTAGLLVAGIFTGCAF
                     LWTVLCYVISNIRGRLSERGLVIVNKVTGVVIFGCGAYAFISLIRLQMV"
     gene            complement(2379..>2743)
                     /locus_tag="VFJ67_00540"
     CDS             complement(2379..>2743)
                     /locus_tag="VFJ67_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET7288893.1"
                     /translation="VSYLYTPFHPAVLRLVKGVVDSAHSKGIPVSVCGEMASQLACVP
                     LLVGMGVDELSMNTHSIPKVKKLLSTITEEESREIARDCLRLKTGAEIRERLTGSLIE
                     KWGEALPPEFLTEIVTNS"
BASE COUNT          578 a          767 c          791 g          607 t
ORIGIN      
        1 tgtacatttt cgccgggcag tggatacacg tccccgcgct cgagctcttc atatcgacgg
       61 tcgagataat aatcctcccc gtcgtcctgg gcgcggggct gaggctcctt ctcgggaaaa
      121 ggatagatca cgtcctgccc gttcttccgg gcgtttcgtc actgtcgata atattcatag
      181 tgggcgtcat cgtcgccgcg aacgccgcct cgatcgcgaa ggtcggagcg ggcgtagccg
      241 ttatagtgat gctccacaac gcagccgggc tcgttctcgg atactcggcc gcgaggctct
      301 cgggcatgga cgtgacgaaa gcgagggccg tgtcgataga ggtaggcatg cagaattcag
      361 ggctcgccgt ggcgctagcg aacctccatt tcagcccgct cgccgcgctt ccggccgcca
      421 tattcagcgt ctggcacaat atatcgggct cggcgatcgc gtggtggtgg aggaggcgca
      481 ccgtttaacc gggcgggcga tactatgact gcgacaacaa ccatcacgaa aagaaaagat
      541 aggttcccga tcgcgggata cctttatctc gtgggcacgg cgctctttac agccctttca
      601 tacgttttcg gcagggccat agacaggagc ttccacccgg cggcgatcac gttctactgg
      661 ttcttcggcg cgtttgtctg ctcggtgttg gtcgctgccg tgatcccctc gcagagggcc
      721 gaggtcagac acctcgggaa atatacgaag atatttatat acagctccgt actgaccgcc
      781 atcggcgctg ctctctggat atcagctata aagacgatcg gcatcccgct aacgtcgttc
      841 ctcatgaagg cgcagaccct cttctcgctc ctcctgggga tgatattcct gggcgagagg
      901 ctcaacaaat gggagacttt cgggatcgtc gtcacgatca cgggcggggt catcgtcgcc
      961 tatcaaagag agctctcgct tctcatcggt actctcacgg ccttgggggc cgcattcttc
     1021 tattcgagcc tatcgttcac agtgaagaaa atgggccaga atctcaacat gctgaccgta
     1081 gccaacctga gggccctcgg cgtgtctata tgcctcatac tgtatcttgc cgctacgggg
     1141 accctccaga cgccgggccc ggtcgacctc gcctttatgg cccttggcgg tataaccggc
     1201 gcttacatag cgaaggcgtg ccagttccag gcgataaagc tgatagacgt ctcccgtacg
     1261 acggccgtgc tgccgctcga atcgttattc gtcgtgatct tctcgtatta catattccac
     1321 gagattccat ccctcataaa gctcataggc ggggcgggga tcatcatcgg cgtcatattc
     1381 ctcgtcgtat tcagggccga gaagcccgag gacctgggcg agggggagtg aggagcgggt
     1441 ctccgcggac atacgtgttt cgacaggctc agcacgtatg ggtttgataa agttcttggc
     1501 cgggcaaaaa gaggatggga aggaacgtat acgagcgcag gttttgcgct acaccatctg
     1561 gagcctgata aggcttatga atgcgtaagc gccgcagccg aagatgacta cgcccgtgac
     1621 cttgttcacg atcacgagcc ctctttcgga cagtcttccc ctgatattgg atattacgta
     1681 gcagagcacc gtccagagga acgcgcagcc cgtgaagatt cccgcaacga ggagaccggc
     1741 cgtcctgtaa tagttagcgg ggttgtgtat gccgattatg gcgaagagcg cgagtatcga
     1801 gagtatgacc atcgggtttg acagggtgag gaggaatgcg gagccgaaca ggccaaagtg
     1861 gctcctcgcg gccttatttt ccgaatacgg ggtaggcttc gaaaaatata cccttacgcc
     1921 gaacacgaag agtattatcc cgccgatcat gtgcacccac gcctctctct gcatgagcat
     1981 ctcggaaatg aacgtgaggc cgaacgcgac tatggccgcg taaacggcgt ctcccgcggc
     2041 cgagccgaag ccggatataa tcccggccgt cctgccctcg ttgatcgtac gctggacgac
     2101 aagcgcgccc acgggcccta tgggggccgt gatgacgatg cctactatta tcccctttat
     2161 gaagaaggtt aaatccatgc ctgaaactca ggtaacaata tacccgaacg ggaattattt
     2221 ttctcaagcg gaagttatga ttgggaaaaa tagatatcct gataataatt atcaatatag
     2281 acgggtgcaa agggctcgga taatttcggg cctggaatta ttgcgggatg gaattttatg
     2341 gaagaccggt cttgcagtca gccggtaagc accggcggtc acgaattcgt gactatctcg
     2401 gtcaggaatt ccgggggcag cgcttcgccc cacttctcta tcagagaccc ggtgagacgt
     2461 tcccttatct cggcccccgt tttaaggcgg aggcagtcac gggcgatctc cctcgactcc
     2521 tcctccgtta tcgtgctgag gagctttttt actttcggga tggagtgcgt gttcatgctg
     2581 agctcgtcga cgcccatgcc cacgagcaga ggcacgcaag cgagctggct tgccatctcc
     2641 ccgcacacgc tgacgggtat gcccttcgag tgagccgaat ccacgacgcc cttgacgagc
     2701 ctcagcaccg ccgggtggaa cggtgtgtag aggtaggata cgt
//
LOCUS       DASSJH010000025         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_113215, whole genome shotgun sequence.
ACCESSION   DASSJH010000025 DASSJH010000000
VERSION     DASSJH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113215"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFJ67_00545"
     CDS             <1..508
                     /locus_tag="VFJ67_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HET7288894.1"
                     /translation="DVNGWRVTSAFGDRKFYKGNYTLRAAAALAGIYGNDAVEAMYPI
                     AYTDSAGGKLDGSKSAYTITFPPGQLPPVNAFWSVTLYDGKTQLLVANPIDRYLINSP
                     MLPGLKKNPDGSLTIYVQKGSPGKDKESNWLPAPDGTFYMVMRLYWPKKEALEGKWSP
                     PPVMPVEK"
     gene            649..1242
                     /locus_tag="VFJ67_00550"
     CDS             649..1242
                     /locus_tag="VFJ67_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288895.1"
                     /translation="MKIRLRIKIVLFLIAAVMLCGQKGIAQQGNETGVELKPSPDVLK
                     EAENPAPPPDAQQKASIGNDGMKVNTENSPGDTDFIWVQQIDVNSDGTAESANLIWDD
                     EDKILYFYYEGDFKCKNGKTGNGAALIGVNGKDNPRQKPAGSGFFAVSLDKDECGAGE
                     AGLWGCRFDPAGNPTVCGIVGLDDKYDDIIILKASEE"
     gene            1296..1694
                     /locus_tag="VFJ67_00555"
     CDS             1296..1694
                     /locus_tag="VFJ67_00555"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HET7288896.1"
                     /translation="MKIPHYRFLIFSALAYVALLASSVSHAGDVNSDSIVGKWQDPDN
                     PAIVQIANKGGYYEGVVVENTDNPALVSTVVFKNLVYDSSEGVWEGTVYSVKKNKDFD
                     AKISMENSGNFTLTVKAGLGSKKFEWARVE"
     gene            1964..>2320
                     /locus_tag="VFJ67_00560"
     CDS             1964..>2320
                     /locus_tag="VFJ67_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288897.1"
                     /translation="MWKGIFLGSICVIITSVAGCGGSYSPKPPEEVSFLDRAETQSRN
                     GLTVTVAVLSREDSEEVFGVDLAANGVQPVWLDIKNDTKKPFFFMPIALDPDYFSPNE
                     VAFMNHFKLNRKANREM"
BASE COUNT          592 a          582 c          651 g          495 t
ORIGIN      
        1 ggacgtaaac gggtggaggg ttacgtccgc gttcggcgac agaaagtttt acaagggcaa
       61 ttacacattg agagcggccg cggcgctcgc cggtatctac gggaacgatg cggtcgaagc
      121 tatgtatcct atcgcataca cggacagcgc gggaggcaag ctcgacggga gtaaatccgc
      181 ttataccatc actttccctc cggggcaatt gcctcccgta aacgccttct ggtcggtgac
      241 tttgtatgac ggcaagaccc agctcctcgt ggcgaacccg atagacagat acctgatcaa
      301 ttctcctatg ctccccgggc tcaagaagaa cccggacgga tcgctcacga tctacgtgca
      361 gaagggatcg cccggcaagg acaaggagtc aaactggctc cctgcgcccg acggcacttt
      421 ctatatggtt atgaggctct actggccgaa aaaggaagcg ctcgaaggga agtggagccc
      481 cccgcccgtc atgcctgtcg agaaatgatc catagattat ggagaacagg ctcgcgtaag
      541 cgagatattt acccgcacgt atgacggtcg tatcttaata tccgttcatg tcgaatccgg
      601 gttgcaaatt atagaagcgg cattaaaatt cgggcaaagg agcgccatat gaaaatacgc
      661 ttacgaataa aaattgtctt gtttctgatc gccgctgtca tgctctgcgg gcagaagggg
      721 atagcgcagc aggggaacga aacgggtgtc gagctcaagc cctctcccga tgtgctcaag
      781 gaagcggaga accccgctcc tccgccagac gcacagcaaa aagcatcgat aggaaatgac
      841 ggaatgaaag tcaacacgga aaactctccg ggcgacacag attttatctg ggttcagcag
      901 attgacgtca acagcgacgg aacagcggag tcagcaaatc tcatatggga cgacgaggac
      961 aaaatactct atttttacta cgagggcgat ttcaagtgca aaaacggaaa aacgggcaat
     1021 ggagcggcgc tcataggcgt taacggcaag gataatccga ggcaaaagcc tgcaggctcc
     1081 gggttcttcg ccgtctcttt agacaaggac gagtgcggcg ccggggaggc gggactatgg
     1141 ggctgcagat tcgacccggc gggtaaccct accgtctgcg gtatcgtggg cctggatgat
     1201 aaatacgacg atataattat cctgaaagcg agcgaagaat aaccgcttcg caggtctatg
     1261 tttaaagtat ccatgaagat atcaaggagg gagttatgaa gataccgcat tatcgttttc
     1321 tgatattctc ggcgctggca tatgtagccc ttttagcttc atccgtgtca cacgcgggtg
     1381 atgttaattc cgacagcata gtcgggaaat ggcaagaccc ggacaatccc gcaatcgtgc
     1441 agatcgcgaa taagggggga tactacgagg gtgttgtcgt cgagaacacg gataaccccg
     1501 ctcttgtttc cacggtggtt ttcaagaacc tcgtgtacga cagcagcgag ggtgtatggg
     1561 aaggcaccgt ttactcggtc aagaagaata aagacttcga cgccaagatc agcatggaga
     1621 acagcggcaa ttttaccctg acggtgaagg ccggcctcgg gtcgaagaag tttgagtggg
     1681 cgcgggtcga ataatcggtc cggtaaatga atccccccgt tcaggtatcc gataccggct
     1741 gcggcgcttt ataccataaa tccgtgcggg ttttagtgcc cggacccggg ctgacagtat
     1801 gtccgccata tctgtctgat attgaataat ttttatgaag gaataaaatt aactgaggcc
     1861 ccggaagcga tttgacggga cggtgatcgg ccgcattttt atcgaaaggg gggcacgtcg
     1921 gtggtttcgt ctcgtccgtc cccgagcccg gatcattata tatatgtgga agggtatttt
     1981 cctaggctct atatgcgtca ttatcacgtc cgtggcgggc tgtggcggta gctattcccc
     2041 gaaaccgccc gaggaggttt cattcctcga cagggccgag acccagagcc gtaacggcct
     2101 caccgtaact gtagccgtgc tcagccgcga agacagcgaa gaggtcttcg gtgttgacct
     2161 ggccgcgaac ggtgttcagc ccgtctggct cgacataaaa aacgatacga aaaaaccttt
     2221 cttctttatg cctatcgcgc tcgatcccga ctatttctcc ccgaacgaag tcgccttcat
     2281 gaaccacttc aagctcaaca ggaaagccaa cagggagatg
//
LOCUS       DASSJH010000026         5806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1086415, whole genome shotgun sequence.
ACCESSION   DASSJH010000026 DASSJH010000000
VERSION     DASSJH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086415"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /locus_tag="VFJ67_00565"
     CDS             complement(<1..1360)
                     /locus_tag="VFJ67_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HET7288898.1"
                     /translation="MRKKHLYILAVLGFMAVFAYTAWASQNAPSESASFPPPLESYND
                     SNLGSIPAILIDRIRQEPFNLVATILFFCAIVHTFLASKFMNIAHKWEHRHEEGIKRG
                     EAEENSVHFGAELFHFLGEVEVVFGIWALVLIFAILLTFGWPTVVNYFSHGVNFTEPA
                     FVVVIMTLAATRPILKLAESMMWKVAGLFGGSLAALWFTILTIGPLLGSFITEPAAIT
                     ISALVLSNKFYDLKPSSKLKYATLGLLFVNISVGGTLTHFAAPPVLMVSGPWNWGTDF
                     MLTHFGWKAFIGILVSNGLYFVFFRRELARLQESFALRTLKDRIESKFVTEELIRKEF
                     DRIKASVELETSILESIREKTELLLVLTRERMEKESLPKLKAEGIDESLIREAFEKRF
                     EEIRLKKIRKYLPGVLPEDMRPEFLDPDWDKREDTVPHWVTAVHLLFMAWTIVNAHHT
                     QLF"
     gene            complement(1551..2114)
                     /locus_tag="VFJ67_00570"
     CDS             complement(1551..2114)
                     /locus_tag="VFJ67_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET7288899.1"
                     /translation="MKHIILACSLMLVVTATYPSFAQYDDTDAGGEETPAQDSNQSPN
                     GGGGEPVIVPQQPYEYYYWPEENLDNFGLSTLGQGEGGSTLGRSLNRKSNIEVNTPKK
                     PAEDEDTENGEPGGEDTDTELYPPDESGLTDGSLSGEQPPPAPKESEFYEWVDENGNV
                     HITNNLGDVPPDQQEEAYKRKSAPGGE"
     gene            2271..2648
                     /locus_tag="VFJ67_00575"
     CDS             2271..2648
                     /locus_tag="VFJ67_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288900.1"
                     /translation="MKILITLASLVIPVVFAAAFPSYTARAEGVSRVSSESETVSAAP
                     GKSVSARCGCPERTKLIGGGGECFGFLVTEGRAGLSRSAPAPDGFSWYVECVNSGTRP
                     GEIRARAWAFCADPGAFGADKKK"
     gene            complement(2658..>5806)
                     /locus_tag="VFJ67_00580"
     CDS             complement(2658..>5806)
                     /locus_tag="VFJ67_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012765668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7288901.1"
                     /translation="ITITLTIVVIYAGLKAFQSLPRLEDPEFTIKEAIITTPYPGASA
                     SEVEKEVTNIIEKAVQELGQLKRVESTSSRGLSTVKAVIKDEYDKKSLPQVWDELRRK
                     VNDYQSQLPPGAGPSLVNDDYGDVYGVYLAITGEGYSYAELKDYADLLKRELLLVKDV
                     KKIVLYGEQPEAVYVEMSRPKMAALEISQQQIYDALRAKNLPADAGDIDVGKEKYPIN
                     PTGEFTSEAMFGDLLVSSQGSKNLVFLRDVADVRRGYIDPPKNMLRYNGKQAIGLAIS
                     TVLGGNVVTMGEGIEEKVKELEPLAPLGMELEVISLQSESVTQSINGFVVNLIEAIVI
                     VVVVLLFAMGLRSGLIIGAVLFITICGTFVFMGMWSITLERISLGALIIALGMLVDNA
                     IVVVDGMRIKMEQGEDAISAANEIVGQTAMPLLGATVVAVLAFAAIGTSKDSTGEYCR
                     SLFQVILISLMFSWVTAVTVTPLLCKYFLIGKKKPGKEDQKAKDPYAGKFFQGYKKLL
                     IAAISKRYITVGIVVGIFILSLIAFGFVKTMFFPTATRPQFFVELYLPEGFRIEDTAK
                     ELEAAEKYLKGLDGVMNVITEIGGGDPRFLLTYVPGKGSSSYAVILVSVEDYNAIDPI
                     FQKTQNELEKLLPNAVVNVRKFLLGPGEGGKIQLRISGPDRMVLRELAGKVKDILNAD
                     PQAKAVRDEWKEKVKVVRPQLEEAQASQLGISRPEVARAFEESFSGTRTGIYRDKSTF
                     EPQLLPIIARAPDYERMNLDTLQSLQIWSTAAGRMIPIGQVISGFNTETEDANIGRRD
                     RVTMIKIHADPRTELPSELLARVKPEIEKALNVDVGKITGQDFGPGDNPFEDFNGEVI
                     PIMDSDQLPLKGLPGYYLAWGGEAEDSARANAGLQKSIPVFFGMMVLIVVFLFNSVRQ
                     PLIIWLTVPLSIIGVTVGLLLFKQPFGFMALLGLLSLSGMLIKNAIVLIDQIDTEIKS
                     GKDRYRSVVDSGVSRLNPVMMAAATTILGMIPLLQDAFFVSMAVTIMFGLLVATVLTL
                     VVVPVLYTIFFKIPNPSK"
BASE COUNT         1176 a         1658 c         1506 g         1466 t
ORIGIN      
        1 cgaagagctg agtgtggtga gcgttgacga tcgtccaggc catgaagagc aggtgcaccg
       61 ccgtcaccca gtgaggcaca gtgtcctccc gcttgtccca gtccggatcc aggaactcgg
      121 ggcgcatatc ctcaggcaaa actcccggaa gatactttct gattttctta agccgtatct
      181 cctcaaagcg tttctcgaaa gcctctcgta taaggctctc gtcaatgcct tccgccttca
      241 gcttcggcag cgattctttc tccatccttt cccttgtcaa taccagaagt agctctgtct
      301 tctctcttat cgattcgagt atgctcgtct ccagctcaac ggatgcctta attctgtcga
      361 attccttccg tatgagttcc tccgtaacga acttcgactc gatcctgtct ttaagcgtgc
      421 gcagcgcgaa actctcctgg agtctcgcca attccctgcg gaagaaaacg aaatacagtc
      481 cgttcgatac gagaatgcct atgaacgcct tccatccgaa gtgcgtgagc ataaaatcgg
      541 tcccccagtt ccagggaccc gataccatca gcactggcgg cgccgcgaag tgtgtgagag
      601 tgccgcccac ggatatattg acgaaaagga ggcccagggt cgcgtatttc agcttcgagc
      661 tcggtttgag gtcgtagaat ttattcgaaa gtacgagcgc cgatattgtg attgctgccg
      721 gctccgttat gaacgagccc agcagcgggc cgatcgtcag tatcgtgaac catagcgcgg
      781 cgagtgagcc gccgaacagg cccgccacct tccacatcat ggactccgcc agcttcagta
      841 tcggccgcgt cgccgcgagg gtcataatga ccacaacgaa cgcgggttcc gtaaaattta
      901 cgccgtgact gaagtagttg acgactgtcg gccatccgaa cgtcagcagt atcgcaaaga
      961 tgagcacgag cgcccatatg ccgaacacca cttccacctc gcccaggaaa tgaaacagct
     1021 ctgcgccgaa gtggacggaa ttctcttcag cctcacccct cttgatgccc tcttcgtgcc
     1081 tgtgctccca tttatgcgcg atattcatga atttgctcgc aaggaacgtg tgcacgattg
     1141 cgcaaaagaa caggatcgtc gccacgaggt tgaacggctc ctggcgtatc cggtcgatca
     1201 gtatggccgg gatgcttccg aggttcgaat cgttatagct ttccagcggg ggcggaaacg
     1261 aggccgattc ggaaggcgcg ttttgagatg cccatgcggt gtaggcgaat acggccataa
     1321 agcccaggac cgctaatata tacaggtgtt tttttctcat gacgctgatt ttgtcctttc
     1381 agatatcagg ggtttcgata aagcccgacg acggcgcttt aaatacccga tgcccatgtc
     1441 gggtctcggc tatcgatatg gttcgcagac gatccggaaa taaaatcgga cccgtctcga
     1501 agttgcggct ttcattgtac acaattcccg gatatttata aagccgggat ctattctccc
     1561 ccgggtgcgg acttgcgttt gtatgcctct tcctgttgat caggcggcac gtctcccagg
     1621 ttgttcgtga tgtggacgtt gccattttcg tccacccatt cgtagaattc gctctccttc
     1681 ggagccgggg gaggctgctc cccggataat gagccgtccg tcaagccgga ttcgtcgggc
     1741 ggatagagct cggtgtccgt gtcctctccg ccgggttccc cgttttcagt atcttcgtcc
     1801 tctgcgggtt ttttaggggt attcacttcg atgttgcttt tccgattcaa actcctgccg
     1861 agggtgcttc cgccctcgcc ctgcccgagg gtcgagagtc cgaagttgtc gaggttctcc
     1921 tccggccagt agtagtattc gtagggctgc tggggaacga ttacgggttc gcccccgccg
     1981 ccgtttggac tttggttact gtcctgagcg ggcgtttctt ccccacccgc atccgtgtca
     2041 tcgtattggg cgaaggacgg gtatgtggcg gtaacaacga gcatcagcga acaagcaagg
     2101 attatatgtt tcatcagggc tggccttctc aggcatgtct ccttctaatt ataacccgta
     2161 tcccagtgta ttcctcggtc cctcgttaaa atcccgttta atcaccattt tgtccgcgtt
     2221 gtgcatacct gtgttagatt attcgtatag ctttttcagg aggcacacaa atgaagatat
     2281 tgatcaccct cgcgtctttg gttattccgg ttgtctttgc ggccgccttt ccgtcttata
     2341 cggcccgagc agaaggcgtg tccagggtaa gctcggaatc ggaaacggtc tctgccgcgc
     2401 cagggaagtc cgtttccgcc aggtgcggct gtcccgagcg cactaagctc ataggcgggg
     2461 gaggggagtg tttcggtttt ctcgtgaccg agggccgggc gggcctttcc agaagcgccc
     2521 ccgcgcccga cgggttttcg tggtatgtcg aatgcgtaaa ttccggaacg cgtccgggag
     2581 aaatacgggc gagggcatgg gccttctgcg cagaccccgg agccttcgga gcggataaaa
     2641 agaaataatc cccctgttta tttggaagga ttcggtatct tgaagaatat tgtatagagc
     2701 accgggacca cgacgagcgt gagcacggtc gcaacgagga gaccgaacat tatggtcaca
     2761 gccatcgata cgaaaaatgc gtcctgaagg agcgggatca tgcctagtat cgtcgtcgcg
     2821 gcggccatca tcacggggtt caaacggctt acccccgagt ccacgacaga ccggtaacgg
     2881 tccttcccgc tttttatctc ggtgtctatc tggtcaataa gcacaattgc gttctttatc
     2941 agcattccgg agaggctcaa aagtcctaat agcgccataa acccgaacgg ctgcttgaac
     3001 agaaggagcc ctaccgttac gcctattatc gagagtggga cagtgagcca tattataagc
     3061 ggctgcctca cggagttgaa taggaatacg actatgagca ccatcatgcc gaagaatacc
     3121 ggtatgctct tttggagtcc ggcgttcgcc cgggctgaat cctcagcttc tccaccccag
     3181 gctaaatagt agccggggag gcctttaagc ggcagctggt cggagtccat tatgggtatt
     3241 acctctccat tgaaatcctc gaaggggttg tctccggggc cgaaatcctg tccggttatc
     3301 ttacctacat cgacgttaag cgccttctcg atctcgggct ttacccgcgc cagcagctcg
     3361 cttggcagct cggtcctcgg gtcggcgtgg attttaatca tagtcaccct gtcccgcctg
     3421 cctatattag cgtcttcggt ttcggtgttg aatccggata tgacctgacc gatgggtatc
     3481 attctccccg cggccgtact ccatatctgg aggctctgta aagtgtcgag attcattctt
     3541 tcgtaatcgg gcgccctcgc aattatcggc agcagctgag gctcgaatgt gcttttgtct
     3601 ctgtaaatgc ccgtccgggt gcccgagaat gactcttcga acgccctcgc gacctccggt
     3661 ctggaaatac cgagctggct tgcctgcgcc tcttccaatt gcggtctcac gaccttgacc
     3721 ttttccttcc attcgtccct caccgctttt gcctgcgggt cggcattcag aatgtctttg
     3781 acctttcccg cgagctccct tagcaccatc ctgtcaggcc cgcttatgcg gagctgaatc
     3841 tttcctccct cgccgggacc aagcaggaat tttctcacgt taacgaccgc attgggaagg
     3901 agtttttcca gttcgttctg cgtcttctgg aatatcggat ctatggcgtt atagtcctcg
     3961 acgcttacga gtatcacggc ataactggag gaccctttgc cgggcacgta tgtaagcagg
     4021 aaccgcgggt cgccgccccc gatctccgtt attacgttca tcaccccatc cagacctttc
     4081 aggtacttct cggccgcctc gagctccttg gccgtgtctt ctatacgaaa tccctcgggg
     4141 aggtagagct cgacaaagaa ctgcggcctt gtagccgtgg gaaagaacat cgtctttacg
     4201 aatccgaatg ctatgagtga caggataaaa atccccacca cgatacctac cgttatgtat
     4261 cttttgctaa tggccgcgat cagcagcttc ttataaccct gaaagaattt tcccgcataa
     4321 gggtcctttg ctttctggtc ttctttgccg ggtttcttct tcccgataag gaaatattta
     4381 cagaggagcg gcgtgacggt taccgccgtg acccagctga acatgagcga gattagaatg
     4441 acctggaaaa gcgatctgca gtactcaccc gtgctgtcct ttgatgtgcc tatcgccgca
     4501 aacgcgagta ccgcgaccac agtcgccccg agaagcggca tcgccgtctg tcccactatc
     4561 tcgttagccg cgcttatagc gtcctctccc tgctccattt tgatccgcat gccgtcgacg
     4621 accacgatcg cgttatcgac gagcattccc agagcgatta tcagtgcacc gagcgagatc
     4681 ctttcgagcg ttatgctcca cattcccata aaaacgaaag tgccgcatat ggtgataaag
     4741 aggaccgcgc cgattatcag cccgctcctt aaccccatcg cgaagagcag tacgaccact
     4801 acgatcacta tagcctcaat gaggttgact acgaacccgt ttattgattg tgttaccgat
     4861 tcggactgca gggaaatgac ttcgagctcc atacccaggg gagcgagcgg ctcgagctcc
     4921 tttaccttct cctctatccc ttcccccatc gttacgacgt taccgcccag gacagtcgat
     4981 atggcgagac cgatggcttg tttcccgttg taacggagca tatttttcgg gggatctata
     5041 tagccccttc tcacgtccgc cacgtccctg agaaagacga ggtttttgct cccctggctg
     5101 ctcacaagga gatccccgaa catcgcctct gaagtgaact ctccggtcgg gttgatcgga
     5161 tatttttcct tccccacatc gatgtcaccg gcgtccgcgg ggaggttctt cgcgcggagc
     5221 gcgtcgtaaa tctgctgctg cgatatctcg agagccgcca tcttcgggcg ggacatctcg
     5281 acgtaaaccg cttccggctg ctccccgtat aggactatct tcttcacgtc ctttacgagc
     5341 aggagctccc ttttgaggag gtcggcgtag tccttgagct ccgcgtagct gtacccttca
     5401 cccgttatag ccagatatac gccgtaaacg tccccgtaat cgtcgttgac gagagaaggg
     5461 cctgcgcccg ggggcagctg gctctggtaa tcgtttacct ttctcctcag ctcgtcccat
     5521 acctggggga ggctcttttt gtcgtattcg tccttgatta ccgcctttac cgtcgagagc
     5581 cctcttgaag aagtggattc caccctcttc aactgcccga gctcctggac agctttttcg
     5641 atgatgttgg ttacttcttt ttcgacttcg gaggcgctgg cacccgggta aggggtagtt
     5701 attatcgctt ctttaatggt gaactcggga tcttcgagcc ggggcaggct ctgaaaggct
     5761 ttaagccccg catagattac tacgatcgtg agtgtaatcg taatga
//
LOCUS       DASSJH010000027         4431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1303363, whole genome shotgun sequence.
ACCESSION   DASSJH010000027 DASSJH010000000
VERSION     DASSJH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303363"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFJ67_00585"
     CDS             <1..204
                     /locus_tag="VFJ67_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7288902.1"
                     /translation="EKRGEGIHHICFLVDDIEAALERLKKEGVRLIDESPRPGAYHSR
                     VAFIHPKAMNGVLIELAEIDKGA"
     gene            complement(234..1577)
                     /locus_tag="VFJ67_00590"
     CDS             complement(234..1577)
                     /locus_tag="VFJ67_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019441125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HET7288903.1"
                     /translation="MNKNLIILISGLAALAVYFFLPVEKNVLKGLSILVFIAVLWFTE
                     ALPITITALLVPILAVLTGIFDVKEALSHFANPVIFLFLGGFALAAALHKHGLDRLIA
                     AQIMKSAGANPLLSVLGLFASTALISMWVSNTATTAIMLPVALGLISNIKDSSISTEE
                     FILLGVAYAASIGGLGTLVGSPPNAITAADLGMDFRDWLFVGMPFVFILMPVMVIILY
                     LVLRPELAGGRILEKDGFKEIAIDKNTYKLAAIFGVTVALWIFSGPISSRLGVDKEFD
                     SIIAILAIFMLVVTGTIKWKEIERFTDWGVLLLFGGGIVLSAVLSETGASKFLADLVR
                     AHLSIHGPFLLILGSVLFVILLSEFASNTATAAIMVPIFLALGQETFRDSPKAIALSI
                     GIAASCGFMLPVGTPPNALVFGTEKVRQRSMIKAGFFVDLACGIVITLVSYYFFF"
     gene            complement(1661..2056)
                     /locus_tag="VFJ67_00595"
     CDS             complement(1661..2056)
                     /locus_tag="VFJ67_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HET7288904.1"
                     /translation="MTETSDFVIGRHKTSQKHYDLVLGINGVTRHWIIPNNIPKKYGE
                     KRIAIEEKGPAGFPPEGRDGQAAEDSWGKGRWEIWDEGSYEIEASSGIKLVIKSSGGR
                     FKGRYLLLVPGWGRWTGKRLWVIEKIRVR"
     gene            complement(2040..2804)
                     /locus_tag="VFJ67_00600"
     CDS             complement(2040..2804)
                     /locus_tag="VFJ67_00600"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET7288905.1"
                     /translation="MVLAVDIGNTNIVFGVFDGGRIMCHWRIGTDKSKTSDEYGILFR
                     ELMKLRDIDPDTIRGAVISCVVPTIQSTVTSAIQTYFNVNPVVVGPGVKTGMPILYHN
                     PKEVGADRIVNGVAAYDKYRSAVIVVDFGTATTFDCVSDKGEYMGGVITPGLVISAEA
                     LYREASKLPRVEIVKPETIIGKTTVESIQSGLIHGYAGLVDGVVEKIKTEMRTEPRVI
                     ATGGVANLIAPESRTIKEVDEFLTLRGLKLIHDRNV"
     gene            complement(2896..>4431)
                     /locus_tag="VFJ67_00605"
     CDS             complement(2896..>4431)
                     /locus_tag="VFJ67_00605"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET7288906.1"
                     /translation="SGGPAGLKIPVSLYEGGKLVSIKEVYIDPQTREAEAEFDINPVT
                     LGRKTYTVSIPVLSDDLIPENNEKSFYTDVIINKIRVLHVAGSPSWDVRFLRKALKRN
                     PNIDLVSFFILRDPSDLVFATENELSLIPFPVNEIFGNELPTFDIVIFQNFNFQPYGI
                     FGFHLRSIRDYVLNDGGAFLMIGGNKSFDSGNYGSTPISEILPVELDYNARTLGDSIK
                     GEEVRPKLTEAGRSHPIMKIVPDKGENEKQWNSMPALEGFNMVEGINPDAVPLLVSPE
                     GEPILAVSKVGAGKAATFLSDSSWRWNFVYGSEGDVSPLYEKFWNRLFLWLVNDPELN
                     DLRVETDKAVYSPGESAEVGIWDLGGDEKGGEHIKTRLTLPDGTEAEIEPARDSSGQR
                     FGARVMLSESGVYKITAGPRDAGYKDTETSEASFIVEPPADELRGVTSNLGLLKSIAG
                     ATGGKYITTRDNPEGLGIGNSRKKTITGYKTVLLWDNPAFFLLIAGFLFSEWSLRRRW
                     GLK"
BASE COUNT          925 a         1257 c         1182 g         1067 t
ORIGIN      
        1 gagaagaggg gcgagggcat ccaccatatt tgttttctcg tggacgatat agaggcagcg
       61 ctcgaaagac tgaaaaagga aggggtgcgg ctcatagacg aatctccgcg tccgggcgca
      121 tatcattcga gggtggcgtt cattcatccg aaagcaatga acggtgtcct tatagagctt
      181 gccgagatcg acaagggcgc ctgaatccgg gaagcagacc gttcaaataa tcattaaaag
      241 aagaaatagt agctgacgag cgtaatcact attccgcagg ccagatctac aaaaaatccc
      301 gcttttatca tggacctctg acggaccttc tcggtcccga acacaagggc gttaggcggc
      361 gtgcccacgg ggagcatgaa accgcacgaa gcggctatgc cgatgctgag cgctatcgcc
      421 ttgggtgagt cccggaacgt ctcctggccc agagccagga agattgggac cattatggct
      481 gcggtcgccg tattactagc gaattcggag agaagtatca cgaacagcac ggagcccaga
      541 ataagcagga aagggccgtg tatggagagg tgggccctga cgaggtcggc aaggaatttg
      601 ctcgcgcctg tctccgacag gaccgcgctc agcacgatgc cgcccccgaa gagcagcagc
      661 actccccagt ccgtgaacct ctctatctct ttccatttta tggtgcccgt cacgaccagc
      721 atgaagatgg cgagtatggc aattatggaa tcgaattcct tgtccactcc gagccgcgat
      781 gatatgggcc cgctgaatat ccagagcgcg acggtaactc cgaatatggc ggcgagcttg
      841 tatgtgtttt tatctatagc tatctccttg aatccgtctt tctccaggat cctccccccc
      901 gccagctccg gtctgaggac gagataaagt attatcacca tcacgggcat caggatgaaa
      961 acgaaaggca tacctacgaa aagccagtcc ctgaaatcca tgcccaagtc ggctgccgtg
     1021 atcgcgttgg gagggctgcc cacgagggtt cccagtcctc cgatgctggc cgcgtacgcg
     1081 acccccagaa gtatgaattc ctcggtcgag atcgagctgt ccttgatatt ggatatgagg
     1141 ccgagcgcta cggggagcat tatggccgtc gttgccgtgt tgcttaccca catgctaatc
     1201 agagcggttg atgcgaacag ccccagtacg gagagaaggg ggttcgcgcc tgccgatttc
     1261 ataatttgag ccgcgatgag acggtcgagg ccgtgtttat gcagtgctgc tgcgagggcg
     1321 aatcctccca ggaagagaaa tatgaccggg ttcgcgaagt ggctgagagc ctcttttacg
     1381 tcgaatatgc ccgtaagcac ggccagtatg gggacgagga gcgccgttat cgttatcggc
     1441 agggcttccg taaaccagag gaccgctatg aaaacgagga tggataagcc tttcagtacg
     1501 tttttttcga ccggcaggaa gaaataaaca gcaagagccg caagtccgct tatgagtatt
     1561 atcaggtttt tattcatgat cgattatgcc gccgggccga gtctattcct tccgcgccgc
     1621 gcgttcaatt cgtaccgccg gggctgttgc ggattgaatg ttaccgcacc cttattttct
     1681 caatgaccca cagtcttttt ccggtccatc tcccccagcc gggaaccagc agcagatacc
     1741 ttcccttgaa tctaccgccc gacgatttta tgaccagctt tatgccgctc gatgcctcga
     1801 tctcgtaaga gccttcgtcc catatctccc atctcccctt cccccagctg tcctcggccg
     1861 cctgcccgtc ccttccttcc ggagggaaac cggcgggtcc cttctcctcg atcgcgattc
     1921 tcttctcgcc gtattttttg gggatgttgt tagggatgat ccagtgtctc gtcactccgt
     1981 taatcccgag gacgaggtca tagtgttttt gggaggtttt gtgccttccg atgacgaaat
     2041 cagacgtttc tgtcatgaat cagtttaagc ccgcggagcg tgaggaactc gtcgacctct
     2101 ttgatggtcc tgctctcggg cgcgatgagg ttcgctaccc cgcccgtcgc gatgacgcgt
     2161 ggctccgttc tcatttccgt tttaattttt tccacgacgc cgtcgacgag gcccgcatag
     2221 ccgtgtatga gtcccgactg gatgctctcg accgtggtct tgccgattat cgtctcgggc
     2281 ttcacgatct ctaccctcgg gagcttcgag gcttcgcggt aaagggcctc ggccgagatc
     2341 acgagaccgg gcgtgattac gcctcccatg tattccccct tgtccgagac gcagtcgaaa
     2401 gtcgtggcag tcccgaagtc gactacgatg acggcgctcc tgtatttatc gtaggcggcc
     2461 acaccgttca cgatcctgtc ggcgcctacc tccttggggt tatgatagag aatgggcatc
     2521 cccgtcttta cgccgggtcc tacgacaacc ggattgacgt taaagtatgt ttgaatggcg
     2581 ctcgttacgg tcgactgaat ggtcggcact acacatgaaa tcacggcgcc acggatcgtg
     2641 tcggggtcga tatccctcag cttcattagt tccctgaaca ggatgccgta ttcgtccgat
     2701 gtctttgatt tgtctgtgcc tatcctccag tggcacatta ttctgccgcc gtcgaatact
     2761 ccgaaaacta tatttgtatt ccctatatcg accgcaagga ccattttttc tccgaatccc
     2821 tataattaaa tccacattat attttgattt caagccggct ggtaaatcag cgagaccgcc
     2881 cgtcaggacg ctcctctact taagccccca ccttctcctg agcgaccact cggagaataa
     2941 gaaacccgct atcagcaaga agaacgccgg gttgtcccag aggaggacgg tcttatatcc
     3001 ggttatcgtc tttttacggg aattccctat gccgagtccc tccggattgt cccgggtcgt
     3061 aatatactta cccccggtcg cgccagctat ggacttgaga agaccgagat tcgatgtcac
     3121 tcccctcaat tcgtctgccg gcggctcgac gataaaagac gcttccgacg tctccgtatc
     3181 cttataaccc gcgtccctgg ggccggcggt gattttgtaa acgccggatt cggacagcat
     3241 gaccctggcc ccgaaccgct gcccggagga gtctcttgca ggctcgatct cggcctcagt
     3301 gccgtcgggc agagtcagcc gggtctttat atgctccccg cccttttcgt ccccgccaag
     3361 gtcccagatg ccgacctcgg cggattctcc ggggctgtat acggctttat ccgtctctac
     3421 tctcaggtcg ttcagctcag ggtcgttcac gagccagagg aatagtctgt tccagaattt
     3481 ttcatataaa ggcgacacgt ccccttcgct cccgtaaacg aaattccatc tccatgagga
     3541 gtccgaaagg aatgtcgcgg ccttgcccgc cccgactttc gacaccgcga gtatgggctc
     3601 tccttcgggg gagacaagta gaggcactgc gtccgggttt ataccttcga ccatgttaaa
     3661 gccttccagc gcgggcatgc tgttccactg cttctcgttc tctcccttgt cgggaacgat
     3721 cttcattatg ggatggctcc ggcccgcctc cgtaagcttt ggccggacct cctctccctt
     3781 gatcgaatcg cctagcgtgc gcgcgttata gtcgagctcc acggggagta tctccgagat
     3841 cggcgtgctt ccgtaattgc cgctgtcgaa gctcttgttg cctccgatca tcaggaacgc
     3901 cccgccgtcg ttgagcacgt aatcccttat gcttctgaga tgaaatccga agatcccgta
     3961 gggctggaaa ttgaaattct ggaatatcac gatgtcgaac gtcggaagct cgttgccgaa
     4021 tatttcgttc acggggaacg gtatgagact cagctcgttt tcagtagcga agacgagatc
     4081 cgaagggtca cgaaggatga agaacgagac gaggtcgatg ttcgggttcc tcttgagcgc
     4141 ttttctcagg aaccggacgt cccaggacgg actgcccgcg acgtggagca cccttatttt
     4201 atttatgatc acgtcggtgt aaaaggactt ctcattgttc tcgggtatga ggtcgtcgga
     4261 cagaacgggt atcgataccg tgtaggtctt cctgccgaga gtgacggggt tgatatcgaa
     4321 ctcggcttcc gcttccctcg tttgcggatc gatgtaaacc tcttttatgg aaacgagctt
     4381 gccgccttca tagagcgaca caggtatctt caatcctgca ggcccgcccg a
//
LOCUS       DASSJH010000028         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_221906, whole genome shotgun sequence.
ACCESSION   DASSJH010000028 DASSJH010000000
VERSION     DASSJH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221906"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFJ67_00610"
     CDS             complement(<1..632)
                     /locus_tag="VFJ67_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7288907.1"
                     /translation="MNISGIFIRRPVMTSLIMAAVLLFGIVGYRSLPVSDLPNVDFPT
                     ILVSASLPGASPETMSSSVATPLERQFSTIEGIDSMTSVSSLGSTQITIQFDLSRELD
                     AAAQDVQTAISRATRLLPQDMPQPPTYRKVNPADQPILYYAVTSPSLQPWELNEYADT
                     IMAQRISMVSGVAQVQIFGAKKYAIRIQLDPNALSSKGIGINEVEEAVNGA"
     gene            complement(673..1782)
                     /locus_tag="VFJ67_00615"
     CDS             complement(673..1782)
                     /locus_tag="VFJ67_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7288908.1"
                     /translation="MPVLVARVEQKDVPVQLNAIGNVEAYSIVEVKPQVDGELSKVYF
                     KEGENVSRGELLFLIDTRPYENVLRQAEANMARDTAMLKKAEQDALRYEDLIKKGVVS
                     AQQYDQVRAEYESLGATVRADRAAVESAKLELEYCSIRSPIDGRIGEILVHEGNTVES
                     RSTLLAVINQTKPVNVNFSVPEQFLALIRSYMADAGDLKVYASVPEGSRTAERKGADA
                     QRIKHGVDLTKENEPTVGTLSFIDNQVNESTGTIMMKAVFQNESETLWPGKFINVTIN
                     LTTQEDAVVIPARAVQTGQQGDYVFVVKPDSTVESRPVVPGARIDGEVVITKGLKPGE
                     LVVTEGQLRLAPGVKVRTNESSQGISGDGSDVSRK"
     gene            2289..2579
                     /locus_tag="VFJ67_00620"
     CDS             2289..2579
                     /locus_tag="VFJ67_00620"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HET7288909.1"
                     /translation="MAYDKLFLMKYSVNSDVATWRVLDGEAVIINNQTSYYYSLNRTG
                     TFVWNLLIDNEMTLDEIVENVSSSYELEGDEIRGDITAILDNLYNEKLLERK"
     gene            2582..2722
                     /locus_tag="VFJ67_00625"
     CDS             2582..2722
                     /locus_tag="VFJ67_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288910.1"
                     /translation="MEKKKTDKTEKTKSKNKPGKSPQREKYEPPKLVKFDKLEKLIVS
                     GE"
     gene            complement(2817..>3291)
                     /locus_tag="VFJ67_00630"
     CDS             complement(2817..>3291)
                     /locus_tag="VFJ67_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HET7288911.1"
                     /translation="SHADNNMDRKKITLAAKELQLEKSGVLGPVSFHGNSMVPFLVDG
                     DELVSVPVAWDEIKTGDIVTYRLSDKFPTYRVIEKHRDKLVVKADDWPELIEVGPGDV
                     LGKVIERRRGGKTLRAGDPSWILYSEYIVWKFRKDKLRSRIRYYKRTIRGIFKKG"
BASE COUNT          744 a          920 c          827 g          800 t
ORIGIN      
        1 gcgccgttta cagcctcttc gacttcgttt attccgattc ctttgctgct gagcgcgttg
       61 ggatcgagct ggatacgtat cgcatacttc ttggccccga atatctgtac ctgcgccact
      121 ccgctgacca tggaaatacg ctgcgccatg atggtgtcgg catactcgtt gagctcccac
      181 ggctggaggc tcggcgacgt taccgcgtag taaagtatcg gctggtcggc gggattaacc
      241 tttctatacg tcggcggttg cggcatgtcc tggggcagga gccgcgtcgc cctcgatatc
      301 gcggtctgaa cgtcctgagc ggccgcgtcg agctccctgc tgaggtcgaa ctggatcgtg
      361 atctgagttg aaccgagcga gctcacagag gtcatcgaat cgatgccctc gatcgtggag
      421 aactgccgct cgagtggcgt tgcgacggag gatgacattg tctccgggct cgctccggga
      481 aggctggcgc taacgaggat ggtagggaag tccacgttgg ggaggtcgct aaccggaagg
      541 gacctgtaac cgacgatccc gaaaagcaat acggcagcca tgataagcga cgtcattaca
      601 gggcgcctga tgaatattcc ggaaatgttc aaatttggat tcccccgttt cccctaattc
      661 tagggttttg cactatttcc tgcttacgtc cgagccgtct ccggaaattc cctgcgagct
      721 ttcattcgtc ctcaccttga ctccgggagc gagcctcagc tggccttccg ttacaacgag
      781 ctccccgggt ttgagccctt tggtgatcac gacttctccg tcgatccttg cgcccggcac
      841 aaccggacgc gattccaccg tagaatcggg cttcacgacg aagacgtaat caccctgctg
      901 gccggtctgg acggcgcggg cgggtattac gaccgcgtcc tcctgcgtag tgagattgat
      961 agtcacattg ataaacttgc ccggccagag cgtctccgac tcgttttgaa atacggcctt
     1021 catcatgata gtccccgtgg attcgttaac ctggttgtct atgaagctga gcgtcccaac
     1081 cgttggctcg ttttccttag tcagatcgac gccgtgttta atgcgctgcg catccgcacc
     1141 cttccgctcc gcggtccggc tgccctcagg cacgctggca tagaccttaa gatccccggc
     1201 atccgccata tagctcctta tcagggcaag aaattgctcg ggtacggaaa aattgacatt
     1261 cacgggtttc gtctggttta taactgcgag gagcgtcgag cgggattcta cggtattgcc
     1321 ttcgtggacg agtatctccc cgatccgccc gtcgatcggg gatcttatag agcagtattc
     1381 gagctcgagt tttgcgctct cgacggccgc tctatccgcc cggaccgttg ctcccagcga
     1441 ttcatactcc gcccggacct ggtcatattg ctgcgctgat accacccctt tcttaatgag
     1501 atcctcgtaa cggagagcgt cctgctccgc ctttttgagc atggcggtat ccctcgccat
     1561 attggcttcc gcctgcctga ggacgttctc atacggcctg gtatctatca ggaacagaag
     1621 ctcgcccctg cttacgttct cgccctcctt gaaatacacc ttcgacagct caccgtccac
     1681 ctgcggctta acctctacga tagaatacgc ttccacgttg ccaatggcgt tcagctggac
     1741 gggcacgtcc ttctgctcaa ccctggctac aagaacaggc actccggctc cctcatcctg
     1801 ccggcccata ttatcggtgg tcttgtcgcc gtcgcctgaa caagctgtga tcagaattaa
     1861 aactgctgta aaaatgaaaa tgtaacccgt ccggaggcga gtcattaaat tctccttccc
     1921 ttattgttat tcggatacag gtggaagcca agattattca caacggcctt acctcgaacc
     1981 agtgacctag taatgctgaa agatcgaccg ttcaatgtga aattcgcgat atttcagcgt
     2041 ccgctaggtc catctttatt atagtacgca agcgaaaacc tgtgcagaaa gtgaaagtaa
     2101 gtgcttactt agaatgtaat aaatattcgc tacaaacccc attatgtcaa tcagctattg
     2161 cttctgcccg tatccggatc agcctgtatt tactaggatt caggcggtct tgaaaacgga
     2221 aaagttacgt ctatgcgatg ggccatggac gccggttagt agggcgataa tttaatattg
     2281 tgaatcgtat ggcttatgat aaattattcc tgatgaagta cagcgtgaat tccgatgtcg
     2341 cgacgtggag ggtcctcgac ggggaggccg tgataataaa caatcagacg agttactact
     2401 atagcctcaa caggaccggc accttcgtct ggaacctgct gatcgacaac gaaatgacac
     2461 tcgacgaaat cgtggaaaac gtaagctcca gttatgagct cgagggggat gagatcaggg
     2521 gagacataac ggccatactg gacaatctat acaatgaaaa gctcctcgag aggaaatgag
     2581 cgtggaaaag aaaaaaacag acaagactga aaaaaccaaa tcaaaaaata aacccggcaa
     2641 aagcccgcag cgcgaaaaat acgagccgcc gaagctggtc aagttcgata agcttgaaaa
     2701 attgatcgtc agcggcgaat agacattttt ggcaggatta cccgcatccc cgacatgata
     2761 tgctgcccgc tagcagtccg gcagacgtaa atcgctccac cccgtccggc agtgcatcag
     2821 cccttcttaa aaattccccg tatagtcctc ttataatatc tgatccttga ccggagctta
     2881 tccttcctga atttccagac tatatattcc gagtataaga tccacgacgg gtcccccgcc
     2941 ctgagagtct ttccgcccct ccgcctttcg ataaccttgc cgagcacatc gccgggcccg
     3001 acttctatga gctcgggcca atcgtccgcc ttcaccacta atttgtcacg gtgtttctcg
     3061 ataacccggt aggtggggaa tttgtcagag agcctgtagg taacgatatc tcccgtcttg
     3121 atttcgtccc acgcgacagg caccgagacg agctcgtccc catcgacgag aaaagggacc
     3181 atgctgtttc cgtgaaaaga tacgggcccg agtaccccgc ttttttcgag ctgaagctcc
     3241 tttgccgcaa gcgtgatttt tttcctgtcc atgttattgt ccgcgtgact t
//
LOCUS       DASSJH010000029         4160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_5671, whole genome shotgun sequence.
ACCESSION   DASSJH010000029 DASSJH010000000
VERSION     DASSJH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5671"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..567
                     /locus_tag="VFJ67_00635"
     CDS             373..567
                     /locus_tag="VFJ67_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7288912.1"
                     /translation="MGQAEGIKTSTEEAEVCRAGLIQRCGLHLVTPIPAISEWGMIAA
                     AVGLGIIGLLITARRKKAAA"
     gene            706..1653
                     /locus_tag="VFJ67_00640"
     CDS             706..1653
                     /locus_tag="VFJ67_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HET7288913.1"
                     /translation="MKRRYNSYHDYLKERFGCRVNKVSLDMGFTCPNRDGSLARGGCV
                     YCNNDSFVPPYARARYSMRDQLTGGMEYLKKRFKAEKFIVYFQAYTNTYGSVSELERM
                     YREALAYEGVVGIAVGTRSDCIDEEKIDMFERLAKECYVSLEYGIESVYDKSLEFMNR
                     GHDYRSVLDAFGMSKGRGFELGAHIILGLPTETRDEMLAMADEVSSLPIDCLKIHNLH
                     IVKGTPLARMYKENPFGLFGYEEYLDFIVRFLERLSPDIIVERFFTDTPRSLLIAPGW
                     NKSHIEVIRGIEEELEKRDTHQGRLYSAGGPHELRSSAF"
     gene            complement(1711..2385)
                     /locus_tag="VFJ67_00645"
     CDS             complement(1711..2385)
                     /locus_tag="VFJ67_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7288914.1"
                     /translation="MIVENSWDFSAFMRDLLTLAGYTSLPVIASVEDAVGTAENSRPD
                     IVLIDAEGDDRLDSLESGEDISERVDIPVIFITGHKDDDTPNRIRAKRLSSYLVKPFD
                     RTELKAAIEIALYKHRTETGAWGGGESNEAYENVNHTARKFSASGINKPDGERDDDLR
                     KEVREFRAEIYRLNMEIAEYKEIIASLNMSLHQPQEDEHTALLIEEEAAPPGIGPVSV
                     IQFQRG"
     gene            2784..3524
                     /locus_tag="VFJ67_00650"
     CDS             2784..3524
                     /locus_tag="VFJ67_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET7288915.1"
                     /translation="MLKSSNPALGEKVFQRLAPGYAGAGEMTINGTINKTLVLLVLVT
                     VPALWIWDMFSKSGIQAVSPWMYGGLIGGLVFAMITIFKKTWAPYTAPVYAILEGLVI
                     GGISAYAESQLKGIVFQAVALTFGTLLSLLVAYRSGVIKVTEKFRLGVVAATGAIFLV
                     YLVSIVLGFFGVSVPLIYSGGTFGILFSLFVVVIAALNLVLDFDFIEQGASQGAPKYM
                     EWYGAFGLMVTLIWLYIEFLRLLTKLRQ"
     gene            3591..4142
                     /locus_tag="VFJ67_00655"
     CDS             3591..4142
                     /locus_tag="VFJ67_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288916.1"
                     /translation="MKTKFIYTAALVVMAAVMLAVPFRTAGAQSGAATPQELGNKVLE
                     AIKAQNKDALKSLVHPQVVAYIEQNNTGDLDKVLDNLLSLKIPGNTEFIVQPMEEVSE
                     YDKATQTLTFRDNALFFPIPPSDLLVLVTETEIPKKDEKGEETKVKAKVGVMVNTVTQ
                     YNKNWYIVLPMKKGTAPAEEKPK"
BASE COUNT          979 a         1064 c         1125 g          992 t
ORIGIN      
        1 ttgaatgatc ttgtatattg gcaggtttgc ataagagact gctcgtggtt ataatctgcc
       61 tgacttatat gcttgtaagc ccatgtactt aacggaggaa ggataatgaa gggtatcaat
      121 ccccttatat tgttaacttt cttgttttta actccttatg cctttgcgca gccgatttct
      181 tgtccttgcg atactcatga attgccgaac gggaccactg gaaatgatat cattgagctt
      241 ctctgtcccg gcggtgagct tgttgaaggc gtctcattcg atataacttc gcatgtagct
      301 cgtatttcgg gagatggtaa gggttattcg gtgcaaatta ttccggatag cacgggctgc
      361 gggataactt ctatgggtca ggctgaaggg ataaagactt ctaccgagga agccgaggtc
      421 tgcagagctg gcttgataca aagatgcgga ctgcatctgg tcacgcccat acctgcgata
      481 agcgaatggg gcatgatcgc ggcggcggtc gggctgggaa taatcggttt gctcattacc
      541 gcgcgcagga agaaagcggc ggcgtaacca agtttaaaaa aatcgcgcag acaaaaggaa
      601 tcttcggctc ccttttctgt ttctaccggg tctgtcccgg gttgacccgg gaactcgctc
      661 ctacaaaaat taatcccata ccctgtataa tatcccgtat aaatcatgaa gaggcgctac
      721 aactcctacc acgattatct gaaagagagg ttcgggtgca gggtcaataa ggtctccctc
      781 gacatggggt tcacgtgccc gaacagggac gggtcgctag cccggggagg ctgcgtttac
      841 tgcaacaacg acagcttcgt ccctccctac gcccgcgccc gctattcgat gcgcgaccag
      901 ctcacgggcg gcatggaata tctcaaaaag aggttcaagg ctgagaagtt catagtctat
      961 ttccaggcgt acacgaacac gtacggcagc gtctcagagc tcgaaaggat gtacagggag
     1021 gcgcttgcct atgagggcgt cgtcggcata gcggtcggca cgaggtcgga ctgcatcgac
     1081 gaggagaaga tagatatgtt cgagaggctc gccaaagagt gctacgtgtc gctcgaatac
     1141 ggcatcgagt ccgtctacga caagtcgctc gagttcatga acaggggaca cgattaccgc
     1201 tcagtgctcg atgcgttcgg gatgtcgaag ggcagggggt tcgagctcgg ggcgcatata
     1261 attctcggcc tgccgacgga gacgagggac gaaatgctcg ccatggccga cgaggtttca
     1321 tcgctcccga tagactgcct taaaattcac aacctgcaca tagtcaaagg cacgcccctg
     1381 gcaaggatgt ataaagaaaa cccgttcggg cttttcggct acgaggaata cctcgatttc
     1441 atcgtgaggt ttctggagag gctctcgccc gatataatcg tcgagcgctt ctttacggac
     1501 acgccgagaa gcctcctcat agctccggga tggaacaagt cgcacatcga agttatcagg
     1561 ggaatcgaag aagagctcga aaaaagggac acgcatcagg ggaggcttta ctcggccggc
     1621 ggcccgcatg agcttcgttc atccgctttc tgatttccgg cccgcgcaat cggaaagaat
     1681 tgccgcgtct ctggttggat ctgaattaaa tcagcctctc tggaattgaa tcacggaaac
     1741 cggtcctatc cccgggggag cggcttcttc ctcgataagg agtgcagtgt gctcgtcttc
     1801 ctgcggttga tggaggctca tgttgagcga cgcgattatt tccttgtatt cggcgatttc
     1861 catgttgagt ctgtatatct cggctctgaa ttccctgact tcttttcgta gatcatcatc
     1921 acgctctccg tccggtttgt ttattcccga tgcggagaat ttacgagccg tgtggtttac
     1981 gttttcgtat gcttcattac tttcaccgcc gccccacgcg cctgtttccg ttctgtgttt
     2041 gtagagagcg atttctattg cggccttaag ctcagtccgg tcgaaaggct taacgagata
     2101 gctcgacaac cttttcgccc tgattctatt gggcgtgtcg tcgtccttat gcccggttat
     2161 aaaaatcacg ggtatgtcga cgcgttccga tatatcttct cccgattcca gagaatcgag
     2221 cctgtcgtct ccttccgcat ctataaggac tatgtccggt ctggaattct cggctgtgcc
     2281 gaccgcatcc tcgaccgagg ctataaccgg cagggacgtg taccctgcta aagtgagcag
     2341 atccctcata aatgctgaga aatcccagct attttcgact atcattatcc tcgcgcccct
     2401 aatcatgaca atcacctcac cttctcagtc tctccgaatt catcggattt agagggatga
     2461 agtgcaagaa atgtgccact atgaaacgct gtaatagccc ttcaacattg aagataacgg
     2521 ccagcaaatc tgtctgaatc gaatcaaacg accaaaaaat gtcatgcatg acaattattg
     2581 ttgcatacgg aggatcggag gatatatcgc ggccggcgta catcagccgg aggtcggggt
     2641 tcggacaagg agcagatatc cgatgcgggc agcggtttct ccgctctccg attatccgaa
     2701 tcagcatcaa cctgctactc aggtgatata cttttctcta acgtaagtta atattcatag
     2761 tcaatatcgg aggtaaaagg taaatgttga aaagctcgaa tcccgcactg ggagaaaagg
     2821 tgttccagag gctcgccccc ggctacgccg gcgcgggcga gatgaccata aacggcacga
     2881 taaacaagac gctcgttctc ctcgtgctgg tgacggtccc cgccctctgg atatgggata
     2941 tgttctcgaa gtccggcata caggccgtga gcccgtggat gtacggaggt ctcataggtg
     3001 ggctcgtttt cgccatgata accatattca agaagacctg ggctccatat acggcccctg
     3061 tgtacgcaat actcgaaggg ctcgtcatag gcggcatatc ggcttacgcc gaatcgcaat
     3121 tgaaggggat agtgtttcag gcggtggcgc ttactttcgg cacgctcttg tcgctgctcg
     3181 tcgcgtacag gtcgggcgtg ataaaggtga cggagaaatt caggctcggc gtcgtggccg
     3241 cgacaggagc gatattcctc gtgtatctcg tgtcgatcgt gctggggttc ttcggggtga
     3301 gcgtaccgct catatactcg ggcggcacgt tcgggatact gttcagcctg ttcgtcgtgg
     3361 tgatagcggc gctcaacctc gttctcgatt tcgacttcat agagcagggc gcctcgcagg
     3421 gcgcgcccaa gtacatggaa tggtacggcg cgttcgggct catggttacg ctcatatggc
     3481 tctacatcga attcctgagg cttctcacga agctgagaca atgacatgtg agcgcgttcc
     3541 cggctgacaa tctttcggct ttcgaaaata actcattacg gaggaagaat ttgaagacca
     3601 aatttattta caccgcggct ctcgtcgtta tggcggccgt tatgctcgcc gttccgttcc
     3661 gcaccgctgg cgcgcagtcg ggcgcggcca cgccccagga gcttggaaac aaggtgctcg
     3721 aagcgataaa ggcgcaaaac aaggacgcac tcaaatcgct cgtccatccc caggtcgtcg
     3781 cgtatataga gcaaaataac acaggggatc tcgacaaagt gctcgacaat ctcctcagcc
     3841 tcaagatacc cgggaacacg gagttcatcg tgcagcctat ggaagaggta tccgaatacg
     3901 ataaagcgac gcagaccctg acgttcaggg acaacgccct ctttttcccc ataccgccgt
     3961 ccgacctgct cgtgctcgtg acggagaccg agataccgaa gaaagacgag aagggcgaag
     4021 aaactaaggt caaggccaag gtcggtgtaa tggttaacac cgtcacgcag tataataaaa
     4081 actggtatat cgtccttccc atgaaaaaag gaacggctcc cgcggaagaa aagccgaagt
     4141 agggtttaca gcattgcggg
//
LOCUS       DASSJH010000030         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_113822, whole genome shotgun sequence.
ACCESSION   DASSJH010000030 DASSJH010000000
VERSION     DASSJH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113822"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFJ67_00660"
     CDS             complement(<1..345)
                     /locus_tag="VFJ67_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7288917.1"
                     /translation="MSIDERVNERLRKAKERWEKDYGSAPKREADFTTISGMEVPPLA
                     TPLDLNSFDYETRLGFPGFYPYTRGIHNSMYMGRLWTMRQFAGFGTPEDTNRRFKYLM
                     EHGQTGLSTAFDM"
     gene            complement(604..1521)
                     /locus_tag="VFJ67_00665"
     CDS             complement(604..1521)
                     /locus_tag="VFJ67_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288918.1"
                     /translation="MRLRKLFKHKSVRVTSVLLFMFLLLGFQYACLDGLNGDSSGNPI
                     NTAIIGLSANPPALFGNAGKDSVIQVNTNLVPDGTPIEFEITFSPTGLRPELRGCLFN
                     STGTVEGNLAFVNYLAGVHKGVGATVEEIAADTATVNISATIHPVDGDEESDFITMTL
                     RGVGIVAPEDQDITVPNPMDTEAEDIFLTLIFNTVGIEPGTIAVVSVSDPALGSLNEG
                     VSEVEVPVLGSSEGGQFIVQYNAFRVGGTQIITARIQLEVPSEFTAFCPLPPAEDLLI
                     EAVVVITQSVESLEPSPSPSSSPTPTMTP"
     gene            complement(1550..>2069)
                     /locus_tag="VFJ67_00670"
     CDS             complement(1550..>2069)
                     /locus_tag="VFJ67_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288919.1"
                     /translation="AVMLTAGVIAVSCGGANPPFAPFGSTVEILDPPGDVAIPNNSLT
                     TLLVQAIVLGPDGEPLNSVRVIWIASFAGQNDLVVDTNGDNVGDARAVQLVNDDACEP
                     KRCALTPITEWFAMGAFVDSPFETLTDDRGIAFVDILISGEDIIDPAFLEASTDSGSV
                     DVVEFSINVPAQ"
BASE COUNT          478 a          598 c          533 g          460 t
ORIGIN      
        1 catatcgaac gctgtgctga gtccggtctg cccgtgctcc atcagatact tgaagcgcct
       61 gttcgtgtct tcaggcgtgc cgaaccctgc gaactgccgc atcgtccaca gacggcccat
      121 gtacatagag ttgtgtatgc cgcgcgtata gggatagaag ccggggaatc ccagccgggt
      181 ctcgtaatcg aaactgttca gatcgagcgg ggtggcgagg ggcggcactt ccatacccga
      241 gatcgtggta aagtcagcct ctctcttcgg ggcgcttccg taatcctttt cccagcgctc
      301 tttcgctttc cttagccttt cattcactct ctcgtcaata gacatccaag cctccggccc
      361 tacctatcct cacaattctc cctgcccgta gaacaacggc acaacgtact ataaatatta
      421 ccaaaattat gcgtatcagg aaacagccgc caaataaata gtaaaaaatg cgggaggcga
      481 gtcaaataat gaccggagtt aacaaagata ttagaattcc tgttgaaaaa tcggacagaa
      541 gtcttctatt acccgaatcc cgggtctcat gtcaatttat tcagaattag cccggtctgg
      601 agattacggg gtcatcgtcg gagtcggaga cgaagacggc gacggtgacg gctcgagaga
      661 ctcgaccgac tgcgttatga caacgaccgc ttctatgagc aggtcttccg cgggcggcag
      721 cgggcagaaa gccgtgaact ctgacggcac ttcgagctgg atacgagcgg tgatgatctg
      781 agttccgccc accctgaagg cgttatactg gacaatgaac tgtccccctt ccgagctgcc
      841 caggaccggc acttccactt cgctcactcc ctcgttcaac gacccgagcg cgggatcgct
      901 tacggatacc acggcaatag tcccgggctc gatgcctacc gtattgaaaa taagcgtgag
      961 aaaaatatcc tcggcttccg tgtccatagg gttgggaacg gtgatgtcct gatcctcagg
     1021 cgccactatt ccgacgcccc tcagcgtcat ggtaatgaaa tcgctctcct cgtccccgtc
     1081 cacgggatgt atcgtggcgg atatattcac ggtcgccgta tcagccgcta tttcctcgac
     1141 cgtcgcaccg actcccttat ggacccctgc caggtaattc acaaaagcca ggttaccctc
     1201 taccgtaccc gtactgttaa acagacaccc ccttaactca ggtctcagtc ccgtgggcga
     1261 gaacgtgatt tcgaattcga taggagtgcc gtcgggaacg aggttagtgt tcacttgtat
     1321 gaccgaatcc ttgccggcgt tgccgaagag agcaggcgga ttcgcgctca gtcctattat
     1381 ggccgtgttg atggggttcc cggagctgtc gccgttaaga ccgtcgagac aggcatattg
     1441 aaatccgaga agcaggaaca tgaacaggag aacagaggtg acgcgcaccg atttatgctt
     1501 gaacaatttt cttagtctca tggaattcca tccctccttg aattaactct tactgcgccg
     1561 gcacgttaat cgagaactcc actacatcga cagagccgct atccgtggac gcctccaaga
     1621 atgcaggatc gattatatcc tcgccgctta taagaatgtc gacgaaggcg attccgcggt
     1681 cgtccgtgag cgtctcgaac ggagaatcga cgaatgcccc catggcgaac cattccgtta
     1741 taggcgtgag cgcgcacctc ttgggctcgc aggcatcgtc gttgacaagc tgcaccgccc
     1801 tcgcgtcacc cacgttatct ccgttagtat cgaccaccag atcattttgg cccgcaaacg
     1861 aggcaatcca aattaccctg acactgttca aaggttctcc gtcagggccg aggactatgg
     1921 cctgcacaag aagcgtggtc agtgaattat tcggaatagc gacgtcaccg ggcgggtcga
     1981 gtatctcgac ggtcgagccg aagggggcga aaggcgggtt tgcccctccg cagcttacgg
     2041 ctatcactcc cgccgtcagc atcaccgca
//
LOCUS       DASSJH010000031         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1195277, whole genome shotgun sequence.
ACCESSION   DASSJH010000031 DASSJH010000000
VERSION     DASSJH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195277"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..846)
                     /locus_tag="VFJ67_00675"
     CDS             complement(88..846)
                     /locus_tag="VFJ67_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7288920.1"
                     /translation="MLTRREFLKAAFAGTIGAAAFPLLSVYEGSKFRFTQLEYNGAWD
                     PRPRDYKRLMSALEVRTSVIASYDRKTLRLSDTDLFNFPFLYLAGDESFEPFAEEERR
                     RLRKYLKLGGTLLIDDASGTDASPFDSQIRDELKRILPENPLSRLAQDHVVYKSFYLL
                     YSASGRVIINPYLEGVTFADEDRTAVFYSRNDLGGAWSEDEFGKWEFECLPGGESQRE
                     LAFRLGINLILYALTGNYKEDQIHVPFIKRRQLL"
     gene            complement(1007..1222)
                     /locus_tag="VFJ67_00680"
     CDS             complement(1007..1222)
                     /locus_tag="VFJ67_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET7288921.1"
                     /translation="MQGKVKWFNSSKGYGFIQSDEIGKDVFVHYSVIEGDGYKSLTEG
                     ETVEFDITEGPKGPQATKVVKLSSGQS"
     gene            1351..2520
                     /locus_tag="VFJ67_00685"
     CDS             1351..2520
                     /locus_tag="VFJ67_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7288922.1"
                     /translation="MNNQGHSALDPAAIEKYRKEFPIVETGIYLDHAGVAPVSLRVIA
                     EVEEFLGEAARRAVMDYERWMERVGAVRALSARLLGADNDEIAFIKNTSHGISIVTSG
                     LDWREGDNVIVFEKEFPSNIYPWLDLKREGVEVRFIPYKGDRIGLDDINGLIDRRTRL
                     VTMSSVQSLNGFMIDLRELGEICRSKGVLFFVDAIQSLGVIPMDVRDCGADFLAADGH
                     KWLLAPEGTGIFYCRRELAPELRPSLIGWKSVKSENDYENLDFTLKENALRFEEGSYN
                     VMGIRALGAALELLFEIGIDSICDRVHDLGDLVMNEAGKRGCRIRTPEDRNERGGIVS
                     FSGDFDPAAVKKKLRESDIVVNNRGGALRLAPHFYNTEDEILKLFEEIDRILERS"
     gene            complement(2592..3047)
                     /locus_tag="VFJ67_00690"
     CDS             complement(2592..3047)
                     /locus_tag="VFJ67_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HET7288923.1"
                     /translation="MNAIISTVLAGIIGTAGMSIVLWGVSQTGIANAAMIRAIGSMFT
                     RSYEDSFGPGLVVHFIAGVIIAFIYVALISLLAPETLAGTIAYGGMIGLAHGIAFGFL
                     LVVAVAEHHPLERFRRAGLEVAIAHLIGHVIYGVLMGTIVGLTGIRFFS"
BASE COUNT          796 a          777 c          844 g          781 t
ORIGIN      
        1 gggagctgtt ttcgcggtta ttaaactcga acaaatgtaa tggcggaggg gacaggcacg
       61 gatttcctcc ctctaatacg aaatcgacta caagagctgc cttctcttaa tgaacgggac
      121 gtgaatctgg tcttctttgt agtttcccgt gagcgcgtaa agaatcaggt ttatccccag
      181 cctgaacgca agctcccttt gagattcgcc gccgggcagg cactcgaact cccatttgcc
      241 gaactcgtcc tcggaccagg ctcctccgag gtcgttcctc gaatagaaga cagccgtcct
      301 gtcttcgtcg gcgaacgtaa cgccttcgag ataagggttg atgattactc tcccgctcgc
      361 ggagtagaga aggtagaacg atttatagac cacatggtct tgcgcaaggc gcgagagcgg
      421 attttcggga aggattctct ttaattcatc ccttatctgt gagtcgaagg gtgaggcatc
      481 ggtgcccgat gcgtcgtcta tcaaaagagt gccgccaagc ttgagatact tcctcagccg
      541 ccttctttct tcctcggcga aaggctcgaa gctttcgtcg ccagcaaggt agaggaacgg
      601 gaaattgaaa aggtccgtat cggagagcct gagggtcttt ctgtcgtaag aggctataac
      661 gctggttctc acctcgagcg cgctcatgag cctcttataa tcccttggcc tcggatccca
      721 tgccccgtta tattcgagtt gagtgaaacg gaatttagag ccttcgtaga cgctcagaag
      781 cggaaaagcc gccgctccga tagttccggc aaaagccgcc tttaggaatt cccttctcgt
      841 aagcacacta ttaaaaatat tgcggacatt cggcaactct gtcaagtata taatagagac
      901 gggtatccgt taacgtgaaa aacggacaaa gaaatccggg ctagcaattt tgaaggaatc
      961 ttcttggaaa gacagagcgc ttgctattcg taaatcgtta tgtctatcag gactgtccgg
     1021 agctcaattt gactactttc gtggcctgag gtcctttcgg cccttccgta atatcaaatt
     1081 ctacggtctc accttcggta aggctcttgt acccgtctcc ttcgataacg ctgtagtgaa
     1141 cgaatacgtc tttgcctatt tcatccgact ggatgaagcc gtaacctttg gagctgttga
     1201 accactttac tttaccctgc ataaagcgat attcctcctg aaaatctact caaaaacctt
     1261 actatcgcac gtttttttta atttgtcaaa tagtatttat tgtatattgt aattgatacc
     1321 atccgttcaa tgcgttagtt ttatccttct atgaataatc aaggacattc cgcgctcgac
     1381 ccggccgcga tagagaaata cagaaaagag ttccctatcg tcgaaacagg catttatctc
     1441 gaccacgcgg gtgttgcgcc ggtgtctctc agggtgatcg ccgaggttga agaattccta
     1501 ggagaggccg cccggagagc cgttatggac tatgagcgct ggatggagcg tgtgggggcg
     1561 gtgagggcgc tctcggcgag gctgctcggc gcggataatg acgaaatcgc tttcattaaa
     1621 aatacatccc acggtatctc catagtcacg agcgggctcg actggagaga aggggataac
     1681 gtaattgtct tcgagaagga attcccgtcc aatatatatc cctggctcga cttaaaaagg
     1741 gagggggtcg aagtcaggtt cataccttac aagggtgacc gcatagggct cgatgatatt
     1801 aacggtctca tcgataggag gacgaggctc gtgacgatga gctccgtaca gtcgttgaac
     1861 ggcttcatga tagacctccg ggagctggga gagatatgcc ggagtaaggg agtccttttt
     1921 tttgttgacg cgatacagag cctcggcgtt attcccatgg acgtcagaga ttgcggagcc
     1981 gatttccttg cagccgacgg ccataagtgg ctccttgcgc ccgaggggac ggggatattc
     2041 tactgccgga gggagctggc tccggaactg aggccgagtc tcatagggtg gaaatcggta
     2101 aaaagtgaaa acgattacga gaacctcgac tttacgctaa aggaaaatgc cctcaggttc
     2161 gaagagggat cttacaacgt gatgggcata agggcgctcg gagcggccct tgagctgctc
     2221 ttcgagatag gcatcgacag tatatgcgac agggtgcacg acctcggaga cctggtcatg
     2281 aacgaggccg ggaagagggg atgtcggata aggacacctg aggacaggaa cgaaagggga
     2341 gggatagtct cgttctccgg ggatttcgat ccggcggctg tcaaaaagaa attaagggaa
     2401 tccgatatcg tcgttaacaa caggggcgga gcgctcaggc tcgctcctca tttctacaat
     2461 acagaagatg agattttaaa attgttcgaa gagatcgata ggattttgga aagatcttaa
     2521 tatcagctga ttatctctat taaaggattt cccaattttg agtaatgagg ttttgaactt
     2581 tttgcgttcg tctaactgaa gaatctgata ccggtcagtc cgactatcgt acccatgaga
     2641 accccgtata taacatgacc gatcaaatgt gcaattgcca cctcgagccc tgctctcctg
     2701 aaccgctcga gcgggtggtg ctcggctact gcgacaacga gaaggaatcc gaacgcaata
     2761 ccgtgagcca gaccgatcat cccgccgtac gctatcgtgc cggcgagcgt ttcgggcgcg
     2821 aggaggctta tcagcgccac atagataaag gcgatgataa cccccgctat gaaatgcacg
     2881 acaagaccgg ggccgaacga gtcttcgtag gagcgggtga acatgctccc tatcgctctt
     2941 atcatcgcgg cgttagctat accggtctgt gatacacccc ataatacgat gctcatgcct
     3001 gcggtaccga tgatccctgc cagaacagtt gaaataatgg cattcacggc acacctcctc
     3061 aatcgaattc gggctcgggt ccggcgttct ctgtaaccgc accgtccgcc ctaatccaat
     3121 cctctgaaac taatgatagc ctgtgtgtgg agacactttc aatgaatttt tttgaggcgc
     3181 gcgccgaatg attgacat
//
LOCUS       DASSJH010000032         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1412110, whole genome shotgun sequence.
ACCESSION   DASSJH010000032 DASSJH010000000
VERSION     DASSJH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412110"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..634
                     /locus_tag="VFJ67_00695"
     CDS             146..634
                     /locus_tag="VFJ67_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288924.1"
                     /translation="MGNTRYSLILAMLLTVLISCGGKPPPEIPERKVLDPSPTKVVEE
                     LLKSWQKEDFKRAFKYVYAPYSDEDGYVIQMKNYFKDNQITILDFQVLGTQIYDRTSI
                     VIVEIKQRVKSLKTGSVIDLTQRSQYDLGIFDDEWKVTSGICIANCIEQEQPLKGTES
                     NK"
     gene            631..1431
                     /gene="dapB"
                     /locus_tag="VFJ67_00700"
     CDS             631..1431
                     /gene="dapB"
                     /locus_tag="VFJ67_00700"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET7288925.1"
                     /translation="MIKVSVMGAAGRMGQRIIYYLSRDDGIKVVGAKESEKHPSLGID
                     AGILAGVGEIGVSITSDIAGSSFGADVIVDFSSPKSTLLNASYASGEGKAMVIGTTGF
                     TDKEESELKELCRVIPCVISANMSIGVNVMLEATRILAQALGDDYDVEITETHHKHKV
                     DSPSGTALMIGQAAAEGLRRDFRKVARFERHGRIGERQSHEIGIQSVRGGDVVGEHTV
                     MFLGDGERIELTHRAFSRDNFARGVLRAVKWVYGKPPGIYSMKDVLEL"
     gene            1481..2590
                     /gene="tgt"
                     /locus_tag="VFJ67_00705"
     CDS             1481..2590
                     /gene="tgt"
                     /locus_tag="VFJ67_00705"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET7288926.1"
                     /translation="MFRFEILKQDSGKGPRLGRIHTAHGAVDTPAFMPVATQASVKSL
                     SPAEVESLGFRMIIVNAYHMYLRPGHRTIEKLGGLHRFMSWPHPITSDSGGFQVLSLS
                     RTRKIKEEGIIFQSHLDGGRHILTPGKCIEIQEALGVDIMMCLDECPPYPSTREYMKR
                     SVGLTSRWAELCKDAKKRHDNGLFGIVQGGVYSDLRRESAEGLTEIGFDGYALGGLGI
                     GEDSDQTCEITESTLEHLPAEKPRYLMGLGKPGDIVDAVSRGVDLFDCVIPTRNARNG
                     TLFTSHGKLVIKNARYAEDEGPIDEDCGCYTCGNFSRAYLRHLFMSKEILVLRLLTLH
                     NLSYYGRLMSDIRESIARDDFSALLNRVKSLGGTD"
     gene            2590..2979
                     /gene="yajC"
                     /locus_tag="VFJ67_00710"
     CDS             2590..2979
                     /gene="yajC"
                     /locus_tag="VFJ67_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET7288927.1"
                     /translation="MHRNLIAIVSLSLLAMGGCPAPGGEGGGGGSPLAFLPFVLIFVL
                     FYFLILRPQQKQSKKKEEMLKSLKRGDNVITAGGIYGRIMNISEDDIVTLEISKGVNI
                     RIARSGIAGPEVQGKEEPKDKEDKSKK"
     gene            2976..>3370
                     /locus_tag="VFJ67_00715"
     CDS             2976..>3370
                     /locus_tag="VFJ67_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288928.1"
                     /translation="MRALRSWIIAIIVCTVLSLLLLLPTIAGDALPGWWGKVFPNRGI
                     RLGLDLRGGIFLVIGVDTEKAVEHELNSIEDFMTEELKKDKVLIKGDEIKGDELKLQF
                     FSKENLDSAAKIANENFRDRADIDESDLS"
BASE COUNT          858 a          842 c          969 g          701 t
ORIGIN      
        1 ctggtcttta cttatctccg gcaccgcctg aagggtgcgg caagacctga agggcatatt
       61 atctgaaagt gttaatttcc ttgatatatc cactccgact acttattcta attgcttaaa
      121 accgctttgc ggttctgaga aacttatggg aaatacacga tattccttaa ttcttgcgat
      181 gttattaacc gtgctcatat cctgcggcgg gaagcccccg cccgagattc ccgagcggaa
      241 agtcctagac ccttccccga cgaaggtggt tgaggagttg ctcaagtcct ggcagaagga
      301 ggatttcaag agggctttca agtatgtcta cgccccttac tccgacgagg acgggtatgt
      361 tatccagatg aagaactact tcaaggataa ccagataacc attctggatt tccaggtatt
      421 ggggacacag atatatgaca gaacatctat agtaatagtg gagatcaagc aaagggtcaa
      481 atctttgaag actggctccg tcatcgacct cactcagagg agccagtatg acctcggaat
      541 ctttgacgat gaatggaagg tgacgtcagg gatttgtatc gcgaactgca tcgagcagga
      601 acagccgctc aaggggacag aaagtaacaa atgataaagg tatcagtgat gggcgccgcc
      661 gggaggatgg gtcagaggat aatttattac ctctcccggg acgacgggat aaaagtagtc
      721 ggagccaagg agtccgaaaa gcatccttca ctcgggattg acgccggtat cctcgcgggt
      781 gtgggagaga taggggtatc cataacgagc gatatcgccg ggtcctcttt cggcgcggac
      841 gtcatagtcg atttctcatc cccgaaatcg acgctcctca acgcctctta cgcctcaggc
      901 gaaggtaagg cgatggttat aggcacgaca gggttcacgg ataaggaaga aagcgagctg
      961 aaagagcttt gccgtgttat cccgtgcgtg atctcggcta acatgagcat cggcgtcaac
     1021 gttatgctgg aggcgaccag gatcctcgcg caggcgctgg gggacgatta cgacgtcgag
     1081 atcacggaga cccaccacaa gcataaggtg gactccccga gcggcacagc gctcatgatc
     1141 gggcaggcgg cggcggaggg tctccgcagg gacttccgga aagtcgccag gttcgagagg
     1201 cacggcagga taggggagcg gcagagtcat gaaatcggaa tacagtccgt gaggggcggc
     1261 gacgttgtag gtgagcacac cgtgatgttc ctcggcgacg gcgagaggat cgagctcact
     1321 cacagggcgt tcagcaggga caatttcgcg agaggagtgc ttcgagcggt caaatgggta
     1381 tacggcaagc cccccggaat ctattcgatg aaggacgtcc tcgagctttg acgtttattc
     1441 cacgccggtt tccgtcgagc gaagccggtt caatcccgat atgttcagat tcgaaatcct
     1501 gaaacaagat agcggtaaag gcccgagact cgggagaata cacaccgcgc acggcgcggt
     1561 cgataccccg gcgttcatgc ccgttgcgac ccaggcctcc gtcaaatcgc tctctcccgc
     1621 cgaggtggag tcgctcgggt tcagaatgat catagtgaac gcctatcaca tgtatctccg
     1681 tcccggccac aggactatag aaaagctggg cgggctgcac aggtttatgt cgtggccgca
     1741 tccgattact agcgacagcg gcggattcca ggtcctgagt ctttccagga ccaggaaaat
     1801 caaggaagag gggataatat tccagtccca tctcgacggc ggccggcata tcctcacccc
     1861 cgggaaatgc atcgagattc aggaggccct gggcgtcgat attatgatgt gcctcgacga
     1921 atgccctccg tatccctcga cgagagagta catgaaaaga tcggtcggac ttacctcccg
     1981 ctgggcggag ctatgcaagg atgcgaaaaa aagacatgat aacggcctct tcggcatagt
     2041 ccagggaggc gtttactctg acctcaggcg cgaatcggcc gaggggctca cggaaatagg
     2101 gttcgacggc tacgccttgg gcggactcgg aataggcgag gattccgatc aaacatgcga
     2161 aatcacggaa tcgacgcttg agcacctgcc cgccgaaaaa ccgcgctatc ttatgggact
     2221 cggcaagccc ggggacatcg tcgacgcggt ctcaaggggt gtcgacctct tcgactgtgt
     2281 cattcccacg aggaacgccc ggaacggcac tctctttaca agtcacggaa aactggttat
     2341 aaaaaatgcc cggtatgcgg aggatgaagg cccgatcgac gaggattgcg ggtgctatac
     2401 gtgcgggaat ttctcgaggg cttatctaag acatctcttc atgtcgaagg aaatactggt
     2461 attgcgtctt ctcactcttc ataacctcag ttactacggc agattaatga gcgatatcag
     2521 agagagcatt gcccgtgatg atttctcggc tctgttaaat cgggtaaaat cccttggagg
     2581 tacggattag tgcatagaaa tctaatagca attgtatccc tgtcgctcct tgcgatggga
     2641 ggatgtccag ctcccggagg cgaaggggga ggcggcgggt cacctctagc gttcctgccg
     2701 ttcgtcctga ttttcgtcct cttttatttc cttatactgc ggccccagca gaaacagtcc
     2761 aagaagaaag aagagatgct gaagagcctg aaacggggtg acaacgtcat caccgccggg
     2821 ggtatttacg gaaggataat gaatatatcg gaagacgaca tcgtgacgct cgagatatcg
     2881 aagggcgtca atatccgcat agcccgctca ggcatagccg gtcccgaagt ccaggggaag
     2941 gaagagccca aggataagga ggataagtcc aagaaatgag agctttaaga agctggataa
     3001 tcgcgatcat cgtatgcacc gttctgtcgc tcctcctcct ccttcccact atcgcgggcg
     3061 acgccctccc tggctggtgg ggaaaggttt tccctaacag aggcataaga ctgggactcg
     3121 acctcagggg aggaatattc ctcgtcatag gtgtcgatac ggaaaaagcg gtcgagcacg
     3181 aactcaacag catcgaagat ttcatgacgg aagagctgaa gaaggacaag gtgctgatca
     3241 agggagacga gatcaaggga gacgagctca aactgcaatt cttttccaaa gagaacctcg
     3301 acagcgccgc aaagatcgca aatgagaatt tcagggacag ggccgacata gacgagtccg
     3361 acctgtcttt
//
LOCUS       DASSJH010000033         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1087291, whole genome shotgun sequence.
ACCESSION   DASSJH010000033 DASSJH010000000
VERSION     DASSJH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087291"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFJ67_00720"
     CDS             <1..680
                     /locus_tag="VFJ67_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7288929.1"
                     /translation="HRWRDGEAGITGSLDDYSFFIWGLLELYESTFDIEYLKTAIELQ
                     NEMKELFWDDRNGGFYFTSHDAEELISRQKEVYDGAIPSGNSVAMLNLLRIGRITGDS
                     SYDEMASKLSRAFSQAVEQAPMAYTQLLAGLDFAIGPSYEVVVAGDPAAEDTKVMLSA
                     LRKKYIPNKVLLFKGTGDAPGITAIADFTKGQSALNGKATAYVCLDHVCNLPTTDLKK
                     ALELLGK"
     gene            918..1094
                     /locus_tag="VFJ67_00725"
     CDS             918..1094
                     /locus_tag="VFJ67_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288930.1"
                     /translation="MEQKKAGKRGRRKIKDLVDMLEDPENSGYIGGDYEDMMDLLEQE
                     LAESEENEDQEEKE"
     gene            complement(1076..>2793)
                     /gene="mnmG"
                     /locus_tag="VFJ67_00730"
     CDS             complement(1076..>2793)
                     /gene="mnmG"
                     /locus_tag="VFJ67_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET7288931.1"
                     /translation="ALGGEMGKAADHSAIQWRRLNTRKGAAVQATRIQADRQAYRAYM
                     KRTLEAEPNLDIKQRMAEGFLAANGRVVGVRTTLGENFYSDAVVVTPGTFPNGLIHIG
                     TTKISSGRAGESAAIDISDSFGSLGFRLGRLKTGTPPRLDGRTIDWDMLEPQPGDENP
                     MPFSFTSGPVMREQLPCYITYTNENTHRVITENLHKSPLYSGEIKGIGPRYCPSIEDK
                     VVKFRDKERHQIFLEPEGVHTYEIYPNGISTSLPLEVQYELVRTIDGLSRVEIMRPGY
                     AVEYDFVDPMQLKPTLETKLVDNLYFAGQINGTTGYEEAASQGLMAGINAALRVKGRD
                     PLVLDRSQAYIGILIDDLVTKGVDEPYRMFTSRAEYRLVLREDNADIRLCEAGYRIGL
                     LGEESFERFYRKKKTISDELLRLGDIKIMPSPSVNGILSGLGSSPLKKPQSLKEILRR
                     PEISYGDLVLITGSGYEAHPGEDFIPQIEMEVKYEGYIKLQVEQIAKFRKLEELSIPG
                     DFSYEGIPGLSREIVQKLSNVRPGSLGQASRISGVTPAAISVLMVYLKKCEFEREAGH
                     HSFSS"
BASE COUNT          637 a          751 c          751 g          654 t
ORIGIN      
        1 tccaccgctg gagagacggc gaagcgggaa taacagggag cctcgatgac tattctttct
       61 ttatatgggg gctgctcgaa ttatacgaga gcacgttcga catagagtat ctgaaaaccg
      121 caatcgagct tcaaaacgag atgaaagaac tattctggga cgacaggaac ggcgggtttt
      181 atttcacgtc gcacgacgcc gaagagctta tatcgaggca gaaagaggtg tacgacggcg
      241 cgattccctc ggggaactcg gtcgcgatgc tgaacctcct caggatcggg aggataaccg
      301 gcgactcgtc ctacgacgaa atggcgtcaa agctctcaag ggcgttctca caagcggtcg
      361 agcaggcgcc gatggcatac acgcagctcc tcgcgggcct cgacttcgcg ataggacctt
      421 catacgaagt ggtcgtggcc ggagaccccg cggctgagga tacgaaagtg atgctttcgg
      481 cgctcagaaa aaaatacatc ccaaacaagg tgctgctatt caagggaacg ggcgacgcgc
      541 cgggcataac tgccatagcc gatttcacta aagggcagtc agccctgaac ggaaaagcaa
      601 ctgcttacgt atgtctcgat cacgtatgca acctgccgac gaccgatttg aagaaagcac
      661 ttgaactctt gggcaagtga gggcattaat ataacttatg agcggccggc cctgacaggg
      721 ttgttgattt tccggattta tgggtataaa tattattaga aagaattttc agacgctgtt
      781 tgtaaatcga ccggccgcgc attatttcat acttgaccag aaagcaggtg tatgaagtgt
      841 ctttatataa ctcccggaat atggaataga gtctgtgtta tagtattcaa ttagttcaaa
      901 caaatgaggt gaacgtgatg gagcagaaga aagcgggaaa gcgaggcaga agaaaaataa
      961 aggacctagt tgatatgctc gaagacccgg aaaactcagg ctacatagga ggagattacg
     1021 aggatatgat ggacctcctc gaacaggagt tggccgaatc ggaagaaaac gaagatcagg
     1081 aagaaaagga atgatgacca gcttctcttt cgaactcaca ctttttcaaa taaaccatga
     1141 gcacggaaat cgcggccgga gtgacgccgg aaatcctgct tgcctgccct aaagaccccg
     1201 gacgaacatt ggacagtttt tgcacgatct ctctcgacaa accggggatg ccctcgtaag
     1261 aaaaatctcc gggtatcgac aactcttcga gcttcctgaa tttagcgatc tgctcgacct
     1321 gaagctttat atacccctcg tatttaactt ccatctcgat ctgcgggatg aaatcctcgc
     1381 ccggatgagc ttcatatccg gagcccgtta tcaacacgag gtctccgtaa gatatctcgg
     1441 gtcttctcag tatttccttg agcgactgcg gcttcttgag aggcgatgag cccaatcccg
     1501 acaatatacc gtttacggag ggagagggca tgatttttat atccccgagc ctcaggagct
     1561 cgtccgatat cgtctttttc ttacgataga acctctcgaa cgactcttcg ccgagcaggc
     1621 cgatcctgta gcccgcttca cagagcctta tgtcggcgtt gtcctctctc agcactaggc
     1681 ggtattcggc cctcgacgta aacatcctgt agggctcgtc tacccctttc gtcacgaggt
     1741 cgtctatcag tatccctata tacgcctgcg agcgatccaa gacgagcggg tcgcgtcctt
     1801 ttacacggag ggcagcgtta atgcctgcca tcagaccctg gctcgccgct tcctcgtacc
     1861 cagtcgtccc gtttatctgg cccgcgaagt aaaggttatc gacgagcttc gtttcgagag
     1921 tcggctttaa ctgcatggga tcgacgaagt cgtactcgac cgcgtagccg ggcctcatga
     1981 tctccaccct cgagagtccg tcaatcgtcc tcacgagctc gtactggact tcgagcggca
     2041 ggctcgtcga gattccgttc ggatatattt cgtatgtgtg tacgccctcg ggctcgagga
     2101 atatctggtg tctctccttg tcgcggaact tgactacctt gtcttcaatt gaggggcagt
     2161 acctgggacc gatgcccttt atctcgcccg aataaagggg cgacttgtgg aggttctcgg
     2221 ttattaccct gtgtgtgttt tcgttcgtat acgtgatata gcaggggagc tgctctctca
     2281 tgacggggcc gctcgtgaat gagaagggca tcggattttc gtccccgggc tggggctcga
     2341 gcatgtccca gtctatggtc ctgccgtcga gcctaggcgg cgtcccggtc tttagcctgc
     2401 ccaatctgaa cccgaggctc ccgaaagaat ccgatatgtc tatcgcggcc gactctccgg
     2461 cgcgcccaga ggatatcttc gtagtcccta tgtgtatgag gccgttcggg aacgtcccgg
     2521 gcgtgacgac gaccgcgtcc gagtaaaagt tctcgccgag cgtagtcctc acgcctacga
     2581 ccctgccgtt cgccgcgagg aaaccctccg ccatgcgctg ttttatgtcg aggttgggct
     2641 cggcctcgag cgtccttttc atgtacgccc tgtaggcctg cctgtcggcc tgtatcctcg
     2701 tcgcctggac ggccgcgccc ttcctagtgt tgagcctcct ccactggatg gcagaatggt
     2761 cggccgcctt ccccatctcc cctccgaggg cgt
//
LOCUS       DASSJH010000034         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_331171, whole genome shotgun sequence.
ACCESSION   DASSJH010000034 DASSJH010000000
VERSION     DASSJH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331171"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VFJ67_00735"
     CDS             complement(<1..883)
                     /locus_tag="VFJ67_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288932.1"
                     /translation="MPTLTAKKPLIALLVLAALIAVSIPLQKRIDDMRGRFRSVEGSP
                     YLSSSSLRKVSLGYNELMADIYWLRALQYFGSKKPTEQNPDLLYHYFDIITDLDPKFV
                     NAYRYGGTFLAEPPPFGLGDTKKGIELFDKGRKNNPGDYKLPLEEAFVYYFYPKDYEK
                     AAELFREASEKPGVSPFRKSSLKGMAASAHVKGGNNELSRKIWEIVFETSPSEERREF
                     ALRRIGEIDTMSLEEKLTQSLREYKKRYGGLPSSLHDLAGAGIVKGQIPEAPVGGGFI
                     IAAKLEAVKSIELSKRKV"
     gene            complement(887..1690)
                     /locus_tag="VFJ67_00740"
     CDS             complement(887..1690)
                     /locus_tag="VFJ67_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7288933.1"
                     /translation="MRKVLSVAHNTFIEAIRDRVLYSMILFAIIMILSTLVFASISAE
                     QYNKIVKDLGLTAISIIGILISVFLGIGLVYKEVERKTVYNIFSKPIKRYEFILGKYL
                     GLGFTIFVITLGMALILFLLVLFIEIRYEGLINFYYGGHYYAEFFNAVYFEYLEFLVV
                     IGIALVFSSFTTPVMSALFTFFAIAIGRFSSDVRLFAEEVKSPVTALFSEAVYRIIPN
                     LEKFDFRSEAVYGGVVDPDLILNTTAYALIYTLALLILAAIIFERKEFK"
     gene            complement(1724..2668)
                     /locus_tag="VFJ67_00745"
     CDS             complement(1724..2668)
                     /locus_tag="VFJ67_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7288934.1"
                     /translation="MSESILNITNLVKDYKTGFTGKKTRVLRDVSFDVRKGEVFGFVG
                     PNGAGKTTTFKLILGFVSPTEGKIELLGEEHSNSGAKSRIGYLPENPYFYDYLTGEEL
                     LRYMGELHGLGRKVLNERTDELLRKVSMEHAKKLQMRKYSKGMLQRIGVAQALVNDPE
                     FLILDEPMSGLDPIGRREIKDLILEEKRKGKTILLSSHMLSDVEALCDRVGIIMGGTV
                     IKIGNIGDLLKEIHTDYEMHIEGSGEDVRQCVKDLRVEMDQRAGYIVLKFDEDMKRKV
                     LEAVTRTPAEIVSIHPLRKSLEGLFVEEARKGNTRNEE"
     gene            complement(2748..>2944)
                     /locus_tag="VFJ67_00750"
     CDS             complement(2748..>2944)
                     /locus_tag="VFJ67_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288935.1"
                     /translation="DADTSAQALRGDFGWEASMTSARINGRKDFSAYADKVIKCTATS
                     QASGAAGTFESGFDDPACDG"
BASE COUNT          605 a          801 c          757 g          781 t
ORIGIN      
        1 ggaccttccg tttcgagagt tcgatgcttt taacggcttc gagcttcgct gctatgatga
       61 atccgcctcc taccggggct tcgggaattt gacctttgac aatcccagcg cctgccagat
      121 catgaagcga cgacggcagt ccgccgtagc gttttttata ctctctcagc gattgcgtga
      181 gcttttcctc gagcgacatc gtgtctattt ccccgattct ccggagagcg aattccctcc
      241 tctcctcgct ggggcttgtc tcaaagacta tttcccagat ctttctcgag agctcgttgt
      301 ttcctccctt cacgtgggcg gatgccgcca tacctttcaa ggaagacttt ctaaatggag
      361 agactcccgg cttttcggag gcttccctga agagctcggc ggccttctcg tagtctttcg
      421 ggtagaagta gtagacgaaa gcctcttcga gaggcagctt gtaatccccg gggttgtttt
      481 tcctcccttt atcgaagagc tcgatcccct tttttgtatc gcccagcccg aagggcggtg
      541 gctcggcgag gaaagtaccc ccgtacctgt acgcgttcac gaacttcgga tcgaggtccg
      601 taataatatc gaagtagtga taaaggagat cggggttctg ctccgtcggt tttttactgc
      661 cgaagtactg gagcgccctc agccagtata tatcggccat gagctcgttg tagccgagcg
      721 aaaccttcct gagggatgag gaagagaggt acgggctccc ttccaccgac cggaacctgc
      781 cccgcatatc gtctatcctt ttctggagcg ggatggatac cgcaataagc gcggccagga
      841 ctaatagtgc gattaacggt ttcttcgcgg tgagtgtagg catctgctat ttgaattctt
      901 tcctctcgaa gataatggcc gcgagtataa ggagggcgag tgtgtatatc agcgcatatg
      961 ccgtggtatt gagaatcaga tccgggtcga cgacccctcc gtagacagct tcactcctga
     1021 agtcgaattt ctcgagattg gggatgattc tgtacacagc ctcggaaaat aacgccgtga
     1081 cgggactctt cacttcctcc gcgaaaagtc tgacgtcgct cgagaaacgg ccgatcgcga
     1141 tcgcgaaaaa agtgaacagc gcgctcatta cgggcgtcgt gaagcttgag aatacgagag
     1201 caatgccgat taccacgagg aattccaggt actcgaaata cacggcgttg aagaactccg
     1261 catagtaatg tccgccgtaa taaaagttta taagcccctc gtacctgatc tcgatgaaaa
     1321 gcactagcag gaacagtatg agggccatgc cgagggtgat cacgaatatg gtaaacccga
     1381 gccccaggta tttcccgagt atgaattcgt acctctttat cggcttcgag aaaatattgt
     1441 aaacggtctt gcgctccact tccttgtaga cgagtcctat gccgaggaat accgagataa
     1501 ggatcccgat gatggatatc gcggtcagcc cgaggtcttt gactatcttg ttatattgct
     1561 cggccgaaat gctcgcgaag acgagcgtgc tcaggatcat tattatcgcg aacaatatca
     1621 tgctatagag aaccctgtcc cttattgcct ctatgaacgt attgtgcgcg acgctcagta
     1681 cttttctcat cttatcctgt atgcgtggtc tatgccgatg gttctactct tcgttacgcg
     1741 tgtttccctt cctggcttcc tcgacgaaaa gcccttccag ggattttctg agcggatgga
     1801 tggacactat ctcggccggc gtgcgcgtaa cggcttcgag gaccttccgt ttcatatctt
     1861 cgtcgaattt gagcacgata tatcccgccc tctggtccat ttcgacccta aggtccttta
     1921 cgcactgcct tacgtcctcg cccgaccctt ctatgtgcat ttcgtagtcg gtgtggatct
     1981 ctttgaggag gtccccgata ttcccgatct tgattaccgt gcctcccatt attatcccaa
     2041 ccctgtcgca gagcgcctca acgtccgaga gcatgtgtga gctcaggagt atcgtcttgc
     2101 ctttccgttt ctcttcgagg atgaggtcct ttatttccct ccggccgatg ggatcgagcc
     2161 cgctcatcgg ctcgtcgagt atcaggaatt cggggtcgtt aacgagagcc tgggcgaccc
     2221 cgatacgctg gagcatgccc ttcgagtact tcctcatctg tagttttttc gcgtgctcca
     2281 tactgacttt tcggagcagc tcgtccgttc tttcgttcag cacttttcgt ccgaggccgt
     2341 gcagctcgcc catgtaccgg aggagctctt cacccgtaag gtagtcgtaa aagtaggggt
     2401 tctccgggag atagcctatc ctgctcttgg ctccggaatt cgaatgctct tcgcctaaga
     2461 gctcgatctt cccttccgta ggggagacaa aaccaaggat caatttgaac gtcgttgttt
     2521 ttccggcacc gttcggaccg acgaagccga acacctctcc ctttcgaacg tcgaatgaga
     2581 cgtccctgag gacgcgggtt ttcttccccg tgaatccggt tttgtagtcc ttgactaggt
     2641 tcgtgatatt gaggattgat tctgacattg tgggacaact aaatatatgt taatcgctgc
     2701 cgggaggcaa tatacggggt ggcatggccg gagtgtatac gtcttcgcta tccgtcgcag
     2761 gccgggtcgt cgaaacctga ttcgaaagtg cccgccgctc cggacgcctg ggatgtcgcg
     2821 gtgcatttaa ttactttatc cgcgtacgcc gaaaaatcct ttctgccgtt gatcctcgcg
     2881 ctcgtcatcg aagcctccca accgaagtct ccgcgcagag cctgagcgct ggtatcggcg
     2941 tcgt
//
LOCUS       DASSJH010000035         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_980085, whole genome shotgun sequence.
ACCESSION   DASSJH010000035 DASSJH010000000
VERSION     DASSJH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980085"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1067
                     /gene="metF"
                     /locus_tag="VFJ67_00755"
     CDS             132..1067
                     /gene="metF"
                     /locus_tag="VFJ67_00755"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET7288936.1"
                     /translation="MRLPEYFKCGDKRPVISFEIFPPKTEGGMKNLEKVLTDLAALGP
                     DFITVTYGAMGSTRERTLEIASLIKKGFGVETACHLTCVGASREGLDTMLSRIYDAGI
                     ENIVAIRGDIPSDDTGSAGHPDGYKYGSDLVRHIRQFEERTLKRRYFGIAVGGYPEKH
                     TEAPDMETDIKNLKLKVDAGADIIITQLFFDNALYFDFVGRARAHGIKNPIVPGLMPI
                     LSVRQIERITSMCGATIPHGLKKKLEACGDDDEKARSIGIAQCIGQSKELIECGAPGI
                     HFYVLNKSEHMKEIISALPVARCRHTGVGIASNIP"
     gene            1088..1771
                     /locus_tag="VFJ67_00760"
     CDS             1088..1771
                     /locus_tag="VFJ67_00760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HET7288937.1"
                     /translation="MPDTVGLFNRVAKRYDALNTFFSLGMDSRWRKRLSDEIRGSLRV
                     LDVATGTGEVAMETVRNLGACRVVGADPSARMLELARRKVALSGLEGRISLARCGAEN
                     LPFRSGAFDAVTIAFGIRNTADPLKSLSEMERVLKPGGKAGIMEFAIPRIRFFAPLYL
                     FYFRNILPLVGSLFGAGSEYKYLSESTSAFPQRESFVKLMEEAGFRTAKTIELMMGIV
                     VIYMGTKKT"
     gene            complement(1814..2449)
                     /gene="pdxH"
                     /locus_tag="VFJ67_00765"
     CDS             complement(1814..2449)
                     /gene="pdxH"
                     /locus_tag="VFJ67_00765"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013190970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET7288938.1"
                     /translation="MEKSESKQYNAFELLEKDLDPDPFKQFGFWFDAAQDAGLVHPDA
                     FALATVSEEGSPSARMLLLKGFDESGFVFYTNSESRKGQELHGNSNAAICFWWDKLER
                     QVRIEGKVERVPGEEADLYFSTRPRGSRLGAWASRQSRVIESREYLDRQYHKFELDYA
                     DMDIPRPGYWNGYRLVPSTIEFWQGRPNRLHDRLRYRKRRDGGWVIERLAP"
     gene            complement(2474..>3178)
                     /locus_tag="VFJ67_00770"
     CDS             complement(2474..>3178)
                     /locus_tag="VFJ67_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HET7288939.1"
                     /translation="HLAGENIAGRWTPEKMKRIEESRVIGTRLLSETLAGLVSKPDVI
                     VSASGIGFYGDRGSEVLTEDSAHGQGFLARLSMDWEAALWPALEAGIRVVNMRLGIVL
                     ATEGGALPKMLTPFRLGLGGRLGSGGQYWSWIAIDDVLSAIYHSLVSGALRGPVNFTA
                     PAPVTNAEFTETLGHALGRPAFFHVPAFALRALLGDMAGETMLTSTRALPSRLLGSGY
                     EFRYPDLKSALNHLLS"
BASE COUNT          726 a          887 c          912 g          653 t
ORIGIN      
        1 tcctctaata catcgagcgc atcagagaga aaaagtagta taacagacaa tgtcggatgt
       61 aacagcatca atttgacaaa gagggcgtga ggtgaaagaa ttaacgggtt aaattcgaga
      121 cagggtcaat aatgcgttta ccggaatact ttaaatgtgg agacaaacgg cctgtaatct
      181 cttttgaaat ttttccgccc aagaccgagg gaggtatgaa gaacctcgag aaggttctta
      241 cagatttagc ggccctcgga ccggatttta taacggtgac atatggggcg atgggttcga
      301 cgagggagag gactctagaa atcgcgtctc taataaaaaa agggttcggc gtggagacgg
      361 cgtgtcacct cacgtgcgtc ggggcatcga gggaagggct cgacactatg ctgagccgta
      421 tatacgatgc ggggattgaa aacatagttg ccatacgggg agacatcccc tcggacgaca
      481 ctggatcagc cgggcacccg gacggatata aatacggctc tgacctcgtg agacacatac
      541 ggcagttcga ggagaggaca cttaaacgga gatatttcgg gatagcagta gggggctacc
      601 cggaaaagca taccgaagcg cccgatatgg aaacggatat caagaacctc aagcttaaag
      661 tcgatgcggg cgcagacatc ataatcacgc agctcttttt cgataacgct ctctacttcg
      721 acttcgtcgg aagagcgagg gcgcacggga taaagaaccc gatcgttccc gggctgatgc
      781 cgatactctc cgtaagacag atcgagagaa taacctccat gtgcggagcg acaattccgc
      841 acggcttgaa gaagaagctc gaagcctgcg gcgacgacga tgagaaagcc cgctctatcg
      901 gtatagcgca gtgcatcggg cagtcaaaag agcttatcga atgcggagcg cccggaatcc
      961 acttttatgt gcttaacaaa tccgagcaca tgaaagagat cataagcgcc ctgcctgtcg
     1021 cccgctgcag gcacacgggc gtcggaattg cctcaaatat tccttaaacc gggaaccccg
     1081 cgaacagatg ccggatacag tcggtctttt caatagagtc gccaagcgct acgacgcact
     1141 caataccttc ttcagcctcg gtatggacag ccgctggaga aagcgccttt cggacgagat
     1201 cagaggctcg ctgagggtcc tcgacgtcgc tacaggcaca ggggaggtgg cgatggagac
     1261 ggtgaggaac ctcggagcgt gcagggtcgt aggcgcggac ccgagcgcgc ggatgctcga
     1321 gctcgcacgg agaaaggtgg ccttaagcgg tcttgagggg cgtatctcgc tcgcgcggtg
     1381 cggcgccgag aatctgccgt tcaggagcgg cgctttcgac gccgtgacca tagccttcgg
     1441 cataaggaac acggccgacc cgttaaagtc gctctccgag atggaaaggg ttctgaagcc
     1501 gggagggaaa gcaggcatca tggaattcgc aataccgagg atcaggttct tcgccccgct
     1561 ttatctcttc tatttcagga acatactgcc gctcgtcggg tcgctcttcg gggcagggtc
     1621 cgaatacaag tacctctctg agtccacttc agcattcccg cagcgcgaga gcttcgtgaa
     1681 gctgatggaa gaagccggat tcaggaccgc aaaaacgatc gagctgatga tggggatagt
     1741 ggttatatac atggggacaa agaagactta atacctgaag taaagtcccg atattcgtta
     1801 cgacccgggt ctttcagggg gcgagccttt cgatcaccca gccgccgtcc cttcgcttcc
     1861 tgtacctgag acggtcgtgg agcctgttgg gccttccctg ccagaactcg atcgtggacg
     1921 gcactagcct gtaaccgttc cagtagccgg gcctgggtat atccatatcg gcgtaatcga
     1981 gctcgaactt gtggtactgc ctgtcgaggt attccctgct ctcaatcacc ctgctctgcc
     2041 gggacgccca ggctcccagg cggctccccc tgggtctcgt ggaaaagtag aggtctgctt
     2101 cctcacccgg gactctctcc acctttcctt ctatccgcac ctggcgctcg agcttgtccc
     2161 accagaaaca gatggctgcg ttcgagttcc cgtggagctc ctgccctttc ctgctctcgg
     2221 agttcgtata gaaaacgaac ccgctctcgt cgaagccctt gaggaggagc atcctcgcgg
     2281 aagggctgcc ctcctcggac accgtcgcga gagcgaacgc gtccgggtgt acgaggcccg
     2341 cgtcttgagc ggcgtcgaac cagaatccga actgtttaaa agggtcgggg tcgaggtctt
     2401 tctcgaggag ctcgaatgcg ttgtattgtt tagattctga tttttccaat ttcagcctcg
     2461 tcgtgcaaga tcgtcaggac agcaggtgat ttaaagcgct tttaagatcg ggatagcgga
     2521 actcgtaacc cgacccgagc agtctcgaag gcagagccct cgtacttgta agcattgtct
     2581 cgccggccat atcgccgagc aaggccctga gcgcgaacgc gggaacgtgg aagaaagccg
     2641 gccggccgag ggcatggccc agcgtctctg taaactccgc atttgtcacg ggcgcgggcg
     2701 cggtgaagtt cacggggccc ctgagtgcgc ctgacacgag tgaatgataa atcgccgaaa
     2761 gcacatcgtc aatcgcgatc cagctccagt attgcccgcc gcttccaagc ctccctccga
     2821 ggccgagcct gaacggcgtg agcatcttcg ggagcgctcc gccttccgtt gcaagcacta
     2881 tcccgagcct catattcacc accctgatgc ctgcctcaag agcgggccag agcgcggcct
     2941 cccagtccat gctgagccgg gcgaggaacc cctgcccgtg cgcgctgtct tctgtcagga
     3001 cctcgctgcc cctgtccccg tagaatccga tacccgacgc cgagacgatc acgtccggct
     3061 tcgacacgag accggcgaga gtctccgaga gtagcctcgt tcctataacg cggctttctt
     3121 ctattctttt cattttttcc ggggtccatc tcccggctat attctcaccg gccagatg
//
LOCUS       DASSJH010000036         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1089265, whole genome shotgun sequence.
ACCESSION   DASSJH010000036 DASSJH010000000
VERSION     DASSJH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089265"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..521)
                     /locus_tag="VFJ67_00775"
     CDS             complement(231..521)
                     /locus_tag="VFJ67_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288940.1"
                     /translation="MRNPVPIVFLLVLLLFPVSNAAFADDYLSEAVTPSGSEDRSDQL
                     YPVDDFEDVIFNREYFYTLEEGKAQYDRSGGSTWKTLDFDLGIFGKNRGNKE"
     gene            996..1673
                     /locus_tag="VFJ67_00780"
     CDS             996..1673
                     /locus_tag="VFJ67_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288941.1"
                     /translation="MKYLITITFALALTVVITSCGGSEDSKTAPSPTPQASAAITPAP
                     SVTPGPGQKQAEDYDFRGTTWGMSMAEVKKAEPYPPRAETADSLTYKGKYKNQITHVN
                     YKFKDDKLFRGGVLYVTKQDSASHYIDNYELIKKDVIAAYGKPVIDGEKQTNPGAVID
                     PDKKAEAVCRGDLMYAAQWNLPRSMVVLMVRGNGKDCFTSLIYLDQKALHSIAEQDGS
                     GSIDVDK"
     gene            1969..2643
                     /locus_tag="VFJ67_00785"
     CDS             1969..2643
                     /locus_tag="VFJ67_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003348490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET7288942.1"
                     /translation="MCGRFVRKEDKERLEGRFGTEVPGDLLLPPEYNIAPSQDCPVVT
                     VEGDRRVLGLMRWGLVPAWAEDLKIGYRMINARAETLDEKRSFKTLLKKSRCFVPASG
                     FYEWKKLAGGGKTPYFFGLRGGGPFAFAGLWTVWHAGREDELRSFTIITTSANEIMAP
                     IHDRMPVILREKDEARWLDPELGKPGDLLPLLAPYPPGEMEFWEVSPYVNSYKNRGEE
                     CVRPVS"
BASE COUNT          671 a          739 c          697 g          570 t
ORIGIN      
        1 gtatacgggg aagtcaatga agattaaaac aaatcaaaat aaacaaacgg agattatgcg
       61 gcgcatatct tcctcaaaaa aaaaccctcc cccctcctta actacaaata atgaggggcg
      121 agggtagaga aggggatatt caataattat tataccagtt atgcgggact aaccaagcga
      181 aatttcgctc ttgttaaaaa attgtgaaga cgggtataaa tgggccgggc ttactcctta
      241 ttgccccggt ttttgccgaa aatgcctaga tcgaaatcga gcgtcttcca cgtgcttccg
      301 cccgagcggt catactgcgc cttcccctct tcgagcgtgt agaaatactc gcggttgaag
      361 atcacgtcct cgaagtcatc gacgggatag agctggtcgc tcctgtcctc agagcccgac
      421 ggagtgacgg cttccgagag ataatcgtct gcgaatgccg catttgagac cgggaacagc
      481 agtaagacga gaagaaaaac tatcgggacc ggatttctca tttcatcacc ccctgtaaat
      541 taccccatac gatactaagg ataactgatt agcgagtgcg tttcatccgt ttttttccgc
      601 cgcgcgcaca cgtagtgtgt tttacgactc catccaacaa ttctgcacaa gccggcaacc
      661 caatccgttc gtactgagct cgtcgaagta tgaaggggtt gggcttccgc tcctgcaaat
      721 ttattagttg attgcgagaa aggttatccg ccgggcctgc cgtactccat gcgttggtat
      781 cggcacccct gccgcgggtg gctcggttcc ccttctcgaa gacgttgcca ccgcagcaag
      841 ggaatgccgt aatgcgcttt acccctctgc ctgctagaag atctacgtat cccgccctta
      901 ccccggatta aaaccccgaa aattctcctc ccctcccccg gcggctcagg taaactattt
      961 ccccgtccgt ataaacacca taggaggaaa aggatttgaa atacctgatt acgattacat
     1021 tcgcgctcgc cctgactgta gtgatcacct catgcggggg cagcgaagac agcaagaccg
     1081 ctccctcgcc gacgccgcaa gcctcggccg cgataacgcc agccccttcg gtaacgccgg
     1141 gtcccgggca gaagcaggcg gaggattacg atttcagggg tacgacatgg gggatgtcca
     1201 tggccgaggt caaaaaagcg gagccctatc cgccgagagc cgaaaccgct gattcgctca
     1261 cttacaaggg caaatataag aaccagatta cgcacgtcaa ctacaagttc aaggacgaca
     1321 agctcttccg cggcggggtc ttatatgtca cgaaacagga cagcgcctcg cactatattg
     1381 acaactacga gcttataaag aaagacgtga tcgccgccta cgggaaaccc gtgatcgacg
     1441 gagagaagca gacaaatccc ggcgccgtca tagatcccga taaaaaggcg gaggccgtat
     1501 gcaggggcga tctcatgtac gcggcgcaat ggaacctgcc ccgctcgatg gttgtcctta
     1561 tggtgcgcgg caacggtaag gactgcttca catccctcat ctacttggac cagaaggcgc
     1621 tgcattcgat cgcggagcag gacggctcgg gcagtatcga cgtagataaa taaccgtgcg
     1681 gcacacacag ataggattta gtttttgtct tccccgacat tacagaattt gcgttaagaa
     1741 aatcgattgt ttacgcgtta aaaaacattt ttgggctgcg gtggaataaa aatgttttag
     1801 cgttaacatg agatttttag taaattctgt tcgtcggatg atttttgctt cttttcatct
     1861 aggaaaagaa ggataaaaca attgcctttc tccatcttca ataaacagga gcggagactc
     1921 ttcaccgtga gccatcaatc aagctttgcg aaatggtaaa attatcccat gtgtggaagg
     1981 ttcgtacgca aggaagataa agagcgcctc gaaggcaggt tcggcaccga ggtccccggc
     2041 gacctgcttc tcccgcccga atacaacatc gccccttcgc aggactgccc cgtcgtcact
     2101 gtcgaaggcg acaggcgcgt gctcggcctc atgcgctggg ggctcgtccc cgcgtgggcg
     2161 gaggacctaa agatcggata caggatgatt aacgcgcgcg cggaaacgct ggacgaaaag
     2221 aggagcttca aaacgcttct caagaaatcc cgctgcttcg tcccggcctc ggggttctac
     2281 gaatggaaga agctcgcggg aggcggcaag actccctact tcttcgggtt gagaggaggc
     2341 gggccgttcg cgttcgcggg gctctggaca gtatggcacg cgggcaggga ggacgagctc
     2401 cgctcgttca ccataatcac gacgtccgcg aacgagataa tggccccgat acacgacagg
     2461 atgcccgtta tactccgcga aaaagacgag gcccgctggc tcgatcccga gctcggaaaa
     2521 cccggtgacc tcctcccgct gctggccccc taccctcccg gtgaaatgga attctgggaa
     2581 gtctccccct acgtcaactc ctacaaaaac cgcggcgagg agtgcgtaag gccggtttca
     2641 taactcacca cgatgcccgg cccgttcgtc ctgcttg
//
LOCUS       DASSJH010000037         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_872805, whole genome shotgun sequence.
ACCESSION   DASSJH010000037 DASSJH010000000
VERSION     DASSJH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872805"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFJ67_00790"
     CDS             complement(<1..207)
                     /locus_tag="VFJ67_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET7288943.1"
                     /translation="MESENKFRSGFITVVGRPNVGKSTLINAVTGMKIAAVSDKPNTT
                     RNRILGIKTLPGAQLVFIDTPGIHK"
     gene            complement(252..1688)
                     /locus_tag="VFJ67_00795"
     CDS             complement(252..1688)
                     /locus_tag="VFJ67_00795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7288944.1"
                     /translation="MIFLLGLGLRLYNLGGESIWFDEAVSVAASKLGFLEQIRWSLSS
                     SDNNPPFYYAFLRLWVLLCGDSEFAVRLPSAVFGSLSILIIYAVGFRLFNRKTALLAA
                     LVLAVSVFHIKFSQEGRAYSLSTLLTLLSYYFFLKCAARGKRSDSVGYIVSSELLIYT
                     HYYGLFIILSQNIYCAWQYISRRESGAPGFRKWIMLQVILGILYIPGLYILYRHSIGI
                     KKGFWLSPPDLKVLFDCFELYSGSVYLLILLTVLSLSAVFNIRGYGISRYPGGDSGPS
                     ARYPGEFNPPYSESVLLLILWLFIPVAVPFLISHVSTPIFFYRYTIVGSLAFYMLASV
                     GAVRTGGRKFIFLAAGLILIFSTINIFGYYERVNKPDWRSGIGHIESEAGAGDVIVLY
                     PWYETEAAGYYAERGDLTIVALTDEFYSADYTRDKDFWLVLCDRWLDDVGHNKRTIEE
                     RIAQGYDVVDTIEFPYLYIYRLERKKNE"
     gene            2000..3796
                     /gene="aspS"
                     /locus_tag="VFJ67_00800"
     CDS             2000..3796
                     /gene="aspS"
                     /locus_tag="VFJ67_00800"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET7288945.1"
                     /translation="MLEHMGDWKRTDYCGALRSQDIGRSVVLMGWVQSNRDHGGVIFV
                     DLRDREGLVQVVFNPQANREIHEKASALRDEWVIAVTGKVTGRSPETVNPNLPTGEIE
                     VLAESLRILNTSRPLPFLIENDIRVDELLRLKHRYLDLRRPHMKDNIVTRHKAVSATR
                     GYLNGEGFIEVETPYLTRSTPEGARDFLVPSRLNPGEFYALPQSPQLLKQTLMISGLD
                     RYYQIVRCFRDEDLRADRQPEFTQIDLEMSYVDENDIMAVVEGILRAIFKETKGIDIP
                     VPFKRMKHEEAMLRYGSDKPDTRFGLELTELTEVFRDSGFQVFSGAIKKGGIVKALKL
                     PGKAGELSRKEIDDLGETAKSLGAKGLAWIRVSGEEWQSPIIKFMSDKEKEDLAGKLE
                     LKDGDIVFFAADTAYIVNLVLSHLRLGLGERLGMIDQGRFDFLWVEDFPLLDYDHTEK
                     RYVAMHHPFTSPKEEDIHLLDSEKGMVRSRAYDVVLNGVEIGGGSIRIFQKDIQEKIF
                     KTIGLTAEQAREKFGFLLDALEFGAPPHGGIALGLDRIVMLITGAGSIRDVIAFPKTQ
                     KGVCPLTEAPSPVEPGQLLELGLRLDVKEKNK"
BASE COUNT         1031 a         1018 c         1096 g          795 t
ORIGIN      
        1 cttatgaatt ccgggagtgt cgataaatac gagctgcgcc ccggggaggg tctttatgcc
       61 gagtatcctg ttccgggtcg tattcggctt gtccgatacg gccgctatct tcatgccagt
      121 gactgcgttt atcagggtcg atttgcccac gttgggccta ccgacaaccg ttatgaaacc
      181 ggacctgaac ttgttttcgc tctccataaa gccgatcacc ctcaaggatt aaaatctatt
      241 ttcatgcctt tctactcgtt ctttttccgc tcgagacgat atatgtagag ataagggaac
      301 tctatcgtat ccacaacgtc gtaaccttga gcgattcttt cttcgatcgt ccgtttattg
      361 tgtcccacat cgtcgagcca gcggtcgcac agtacgagcc agaagtcctt atcccgcgta
      421 tagtccgcag agtaaaattc atccgtgaga gccacgatcg tcaaatcacc ccgttcggca
      481 taataccccg cagcctccgt ttcgtaccag ggatatagaa ctataacatc cccggctccg
      541 gcctcggatt cgatatggcc gatgcccgac ctccaatcgg gcttgttcac acgttcataa
      601 tatccgaaaa tattgatggt tgaaaaaatc aggatcagtc ccgcagcgag aaaaatgaat
      661 ttcctgcctc ccgttcttac cgccccgacc gaggcgagca tataaaatgc gagagacccg
      721 actatcgtgt atctgtagaa aaaaatcggc gtcgaaacgt gggatatcag gaaaggcacg
      781 gctaccggga tgaataacca cagaatcagg agcaggacgc tctccgagta aggcgggtta
      841 aattcccccg gataccgggc ggagggcccg gagtctcctc cgggataccg ggaaattccg
      901 tagccccgaa tattgaatac ggccgacaga gaaagcacag tcagcaatat gaggagatat
      961 accgatcccg aatacagctc gaaacagtcg aataagacct ttaggtcggg aggggaaagc
     1021 cagaatcctt tcttgatacc tatcgaatga cggtacagta tatatagccc cggaatatat
     1081 aggattccga gaatcacctg gagcataatc cactttctga agccgggcgc tcccgattcc
     1141 ctgcgggaga tgtattgcca cgcgcagtat atgttttgag agagtatgat gaagaggccg
     1201 tagtagtggg tgtagatcag aagctcgctc gacacgatgt aacctactga atcggaacgc
     1261 ttgccgcgag ccgcacactt taggaaaaaa taatatgaaa ggagcgtgag aagcgtagag
     1321 agactataag cccttccttc ctgagagaac ttgatgtgaa aaacggaaac agccagaacg
     1381 agcgccgcga gcaaggccgt ctttctgttg aacaggcgga agccgacagc gtaaataatc
     1441 aggatgctta acgacccgaa aacggccgag ggaagcctga ccgcgaattc ggagtcgccg
     1501 cacaggagaa cccagaggcg gagaaatgcg tagtaaaagg gcggattgtt atcgctcgaa
     1561 gacaggctcc accttatctg ctcgaggaac ccgagcttcg acgccgcgac cgaaaccgcc
     1621 tcgtcgaacc atatactctc tcccccaaga ttataaagcc tgaggccgag cccgagtaaa
     1681 aatatcaata ttaataagta gttgtttctt agaaaaaccg acgcagacgt ctcaagcacg
     1741 cgagaattca ttttacggaa accggaatca cgccgcgtaa agcccggacc tgcgccctcc
     1801 cgggaatatg tgtacgatcc tgaacgccat atgctgcatc cgacaattgt aaacactaat
     1861 gcactccttt gccagcggaa tgatgtgcag cgcccgcagc cggagtgccc ggattcgggc
     1921 cgtccgtctt gccaaatccc aagcaaaggt taagatttgt gggttttaag acatatttat
     1981 acgataagga gcatttctca tgcttgaaca tatgggagac tggaagagga cggattactg
     2041 cggggcgctg aggtcgcaag acatagggag aagcgtcgtg ctcatgggct gggtacaatc
     2101 gaacagggac cacggaggag tgattttcgt cgatctcaga gacagggaag gattggtgca
     2161 ggtggttttc aatccgcagg cgaacaggga gatacacgag aaggcctcgg cgctccggga
     2221 cgaatgggtt atagcagtca cgggcaaagt gaccggcagg tcccccgaga cggttaaccc
     2281 caaccttccg acaggtgaga tagaggtcct ggccgagagc ctcaggatac tgaacacatc
     2341 gagaccgctc ccgttcctca tagaaaacga catcagggtg gacgagcttc tgaggttgaa
     2401 gcacaggtat ctcgacttga gacgccccca tatgaaggac aatatcgtaa cccgccacaa
     2461 agcggtttcc gcgacgaggg gctacctcaa cggggaaggc ttcatcgagg tggaaacccc
     2521 ttatctgacg aggtcgacgc ccgaaggggc gagggatttt ctcgtcccga gcaggctcaa
     2581 cccgggggaa ttctacgcgc tcccgcagtc tccccagctc ctcaagcaga cgctcatgat
     2641 atcggggctc gacaggtact accagatagt gcgctgcttc agggacgaag acctgagggc
     2701 cgaccgccag cccgagttca ctcagataga cctcgaaatg tcttacgtgg acgagaacga
     2761 cataatggcg gtcgtggaag gcatactccg ggccatattc aaagagacta aaggcataga
     2821 catacccgtc ccgttcaaga gaatgaaaca cgaggaggcg atgctcagat acgggtccga
     2881 caaaccggat acgaggttcg ggctcgagct cacggagctt acggaggtgt tcagggactc
     2941 cgggttccag gtattcagcg gggcgataaa aaagggcggt attgtcaagg ccctcaagct
     3001 cccggggaag gccggggagc tttcgaggaa ggaaatagac gacctcggag agaccgccaa
     3061 gtccctcggc gcaaaggggc ttgcatggat ccgcgtatcg ggagaagaat ggcagtctcc
     3121 cataatcaag ttcatgagcg acaaggaaaa agaagacctg gccgggaaac ttgagcttaa
     3181 ggacggcgac atcgtgttct tcgctgccga tacggcttat atcgtgaacc tcgtgctgtc
     3241 gcacctgagg ctcggtttag gggagcgcct tggcatgata gaccagggga ggttcgattt
     3301 cctctgggtc gaggactttc ctctcctcga ttacgaccat acggaaaaac gctatgtcgc
     3361 catgcaccac ccgttcacct ctccgaaaga ggaggacatc catcttctcg attcggagaa
     3421 gggtatggtg aggtcgaggg catacgacgt tgttctgaac ggcgtagaga tcggcggcgg
     3481 cagcatcagg atattccaga aagacatcca ggagaagata ttcaagacca taggcctcac
     3541 ggctgagcag gcgagggaga aattcgggtt tcttctcgac gcgctcgaat tcggggcacc
     3601 gccccacggc ggtatcgcgc tcggactcga caggattgtg atgctcatta cgggtgcggg
     3661 ctccatcagg gacgtaatag ccttccccaa gacacagaaa ggggtatgcc ctctcactga
     3721 agcaccgtcc cctgtagagc cgggccagct cctcgagctc ggtctcaggc tcgacgtcaa
     3781 agaaaagaac aagtgaggcg gctcccttgt ccacacgacg cagaggacgg cagacgagcg
     3841 gacgattgga atgggcctaa gccggaagcg gcggctgtct gaagaagcta cttgctcagg
     3901 tagtcctcgt tatttttagg aagaataatg aaataattct
//
LOCUS       DASSJH010000038         2824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_765891, whole genome shotgun sequence.
ACCESSION   DASSJH010000038 DASSJH010000000
VERSION     DASSJH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765891"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFJ67_00805"
     CDS             <1..389
                     /locus_tag="VFJ67_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288946.1"
                     /translation="VFTELGKEAGERLGLSSGLARAFGGGYSWVRTGWFDLINLEIDD
                     LEILEDHLTRKILFFRLFFPLKEDFGWVFDSPDVTSSMKSIFDRFSSWQTDPVGYDKD
                     LESYKNEIAPIREGLASALEIGSGRC"
     gene            900..1391
                     /locus_tag="VFJ67_00810"
     CDS             900..1391
                     /locus_tag="VFJ67_00810"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET7288947.1"
                     /translation="MVLIIMGVSGAGKTTVGRVLARKLGWNFFDADDLHSEKNKEKLK
                     NGIPLTDEDRLPWLGALRGLIWGLDAPSVIACSALKKSYRDKLAGSGRDVRFVYLGGD
                     KELIRGRLERRKGHFAGAGILESQLETLEEPDDALREDIRHDPETIADDVIRRLKLKA
                     FSG"
     gene            complement(1397..2263)
                     /locus_tag="VFJ67_00815"
     CDS             complement(1397..2263)
                     /locus_tag="VFJ67_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET7288948.1"
                     /translation="MKDKKFGKVAIVGLGLIGGSLAGALKKSGKVADVTGIDIDRGSI
                     DYALRKKIIDHGYTNIGAGVSGAEVIVIATNVGDIVKTAESIIPLARKGAVITDVGSV
                     KGRIVSKLGKLTPGHLRFVGGHPIAGTENSGVKASQPGLFRGKRFIITPTSKTDPGAV
                     KMVSDLWRAAGAEIYEMDPRAHDRVFGFVSHLPHIVAYSLVDSILSAGDSKSLFDFAG
                     GGLRDYTRVAASSPEMWTDIFTANRVSALKAIRKFRKSLDKIETAIKKKDAGSLRKIL
                     SKSARAKREDIK"
     gene            complement(2260..>2824)
                     /locus_tag="VFJ67_00820"
     CDS             complement(2260..>2824)
                     /locus_tag="VFJ67_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7288949.1"
                     /translation="WFLGNVPDDLLVVIDEAYFEYVEEPEYPDSLSYQDTGKSILTVR
                     TFSKIYGLAGLRIGYGVAGEGMISAMQRARHPFNTSSVAQEAAMAALDDHGHVDKSRK
                     INREGLAYLKDELGKLGISYTPTYTNFILIDLKKDPMPLYNALLREGVIVRPVGGYGL
                     MTHLRVTIGLPGENERFVRAMKKVMNL"
BASE COUNT          603 a          742 c          786 g          693 t
ORIGIN      
        1 acgtgtttac cgaattgggg aaagaagcgg gagagaggct cgggctgtct tcagggctgg
       61 ccagggcatt cggcggaggc tacagctggg tgaggaccgg gtggttcgat ctgataaacc
      121 tcgagatcga cgatctcgaa atcctggaag atcacctgac ccgtaagata ttatttttca
      181 ggctgttttt cccgcttaaa gaggacttcg gctgggtctt cgactcgccg gacgttacct
      241 cgagcatgaa atccatattc gacaggttct catcctggca gacggacccc gtgggctacg
      301 acaaggacct cgagtcttat aagaatgaga tagcgcccat tagagagggt cttgcatcgg
      361 ccctcgaaat agggtccggc cgttgctgat ctcggtaaag accgcgtgag ccctgtgttt
      421 gggggaagaa attccggttc caaacccgaa ttatccgagt ataatttcac taggtcatac
      481 gaataaagtt taagcgagga tataaaatgc gcgcttatat tactctggcc gcggtcctgt
      541 tcgtcgccct tttcatcgcc ggctgcaaat gcaatgtcgg ctgaattctt tcaggcggcc
      601 gcgcggacgc atccgtttga tggatttccg ggccgataaa gattaatcgg caacctggtt
      661 atcccgcgtc acggaacaga tgaatcggga ataagtattt ggagagcatg tttttaattg
      721 tataattcca gataggtgtg aataaatact cgaaaaggat accggaatgc gcttatacct
      781 gacattgttt tcgattcttc tcgtcactct ccttgtcacg gcctgccagt gcggcatcgg
      841 ctgagggctt acgggaaggg ccgcatacct gaaaattaac ttattatatt cccggaaaca
      901 tggttctgat aatcatgggt gtctcagggg cgggtaagac taccgtcggg agagtcctgg
      961 cccgcaagct gggatggaac ttctttgatg cggacgacct ccattccgaa aagaataaag
     1021 agaagttaaa aaacggcata cctctcactg atgaggacag gctgccgtgg ttaggggcgc
     1081 tgcgcgggct tatttggggg ctggacgcgc cttcagtgat cgcgtgctct gcgctcaaga
     1141 aaagctacag ggataagctc gctggaagcg gccgcgatgt gcgtttcgtg tatctaggcg
     1201 gcgataagga gcttatccgg gggaggctcg agagaagaaa ggggcatttt gcgggagccg
     1261 gtattctcga aagccagctc gagacgctcg aagagcccga tgacgcgtta cgggaggata
     1321 taaggcacga tccggaaacc atcgcggacg acgtcatcag gagactgaaa ctcaaagcgt
     1381 tcagtggctg atcgagctat ttaatgtcct ctctctttgc ccgagcgctc ttcgagagga
     1441 tcttcctgag cgatcccgca tcctttttct ttatagcggt ttctatcttg tcgagggatt
     1501 tcctgaattt cctgatggct tttagcgcgc taaccctgtt ggccgtaaat atatctgtcc
     1561 acatctctgg cgagctggcc gcgaccctgg tgtagtccct gagcccccct ccggcaaagt
     1621 cgaaaagtga tttcgaatcc cccgccgaaa gtatcgaatc gacgagtgaa taagcgacta
     1681 tatgcggaag gtggcttacg aagccgaaga cgcgatcgtg cgcacggggg tccatctcat
     1741 atatctccgc gcctgcggcc ctccataaat cggagaccat tttaaccgcc ccggggtcgg
     1801 ttttggatgt gggggtaatt atgaacctct tacctctgaa aagtccgggt tgggaggcct
     1861 taactcccga attctccgtt ccggctatcg ggtggccgcc gacgaagcgg aggtgtccgg
     1921 gggtgagctt gcccagcttg ctcactatcc tgcccttcac gctcccgacg tcggtaatca
     1981 cggctccctt acgggcgagt gggattatcg attcggctgt tttgactata tccccgacgt
     2041 tagtcgcgat cactatgact tccgcgccgg aaacgcccgc gccgatattc gtatagccgt
     2101 ggtcgattat ctttttccga agtgcgtagt cgatcgaccc cctgtcgatg tctatgcccg
     2161 tgacgtccgc aaccttaccc gatttcttga gcgctccggc gagggagccc ccgatgagac
     2221 cgagcccgac tatggctacc ttcccgaatt tcttatcctt cataggttca tgaccttctt
     2281 catagccctc acgaaacgct cattctcccc gggaagcccg atagtcacgc ggagatgggt
     2341 catgagtccg tatccgccga ccggcctcac tatcacccct tccctgagaa gcgcgttata
     2401 gaggggcatg gggtctttct tgaggtctat gagaataaag ttggtataag tgggcgtgta
     2461 gctgattccg agcttcccga gctcgtcttt cagatacgcg agcccctccc tgtttatttt
     2521 tctcgattta tcgacgtgcc cgtggtcatc cagtgccgcc attgccgcct cctgcgcgac
     2581 ggagctcgta ttgaacgggt gtctggccct ctgcatggcc gatatcatgc cttcgccggc
     2641 gacgccgtag cctatcctga gccctgcgag cccgtatatc ttggagaacg tcctcacagt
     2701 gagaatggat ttgcccgtgt cctgatatga gagggaatcg gggtattcgg gttcctccac
     2761 gtactcgaag taggcttcgt ctatcacgac caggaggtcg tcggggacgt tacccaaaaa
     2821 ccat
//
LOCUS       DASSJH010000039         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_766040, whole genome shotgun sequence.
ACCESSION   DASSJH010000039 DASSJH010000000
VERSION     DASSJH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766040"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            506..727
                     /locus_tag="VFJ67_00825"
     CDS             506..727
                     /locus_tag="VFJ67_00825"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HET7288950.1"
                     /translation="MANKKEAAPKKTAVKKVATPKTTSKKSSPAKESVQDKIARVAYE
                     LYEKSGYVHGRDVEHWIEAERIVLGKKKK"
     gene            1028..>2790
                     /locus_tag="VFJ67_00830"
     CDS             1028..>2790
                     /locus_tag="VFJ67_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011319522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HET7288951.1"
                     /translation="MNTGTKLTAEEKRLRLDTEKKAYWRRWGPYLSERQWGTVREDYS
                     PGGTAWEYLPHDHARSRAYRWGEDGLAGISDNHQRLCFSVALWNGHDPILKERLFGLT
                     GDEGNHGEDVKEYYYYLDNTPSHAYMKCLYKYPQKAYPYDDLVEVNKQRSRHEPEYEL
                     IDTGVFDDERYWDVFVEYAKKTPEDIHILITVANRGPSEAVLHLLPTLWFRNTWSWKG
                     GEKPSLRYAESEDKLKVIEASHPTLGNRWLYCDNPGGLLFTDNETNFERIYGSPNDTP
                     FAKDGINDYIVGGRESAVNGKCTGTKVSAHYTLTVPPGKSVTVKLRLSDTINLIDPFG
                     KSYDTVFAMRKKEADEFYRRVCPYTLPEDLASIQRQAFAGMLWTKQFYYYVVQEWLDG
                     DPLDPPPPESRKQGRNHNWIHVYNDDILSMPDKWEYPWFAAWDLAFHTVTFSLIDPDF
                     AKRQLMLLTREWYMHPNGQIPAYEWAFSDVNPPVHAWAAWRVYNIEHKMYGRRDIHFL
                     ERVFQKLLLNFTWWVNRKDAEGKNVFEGGFLGLDNIGVFDRNAKLPSGVILEQADGTS
                     WMGMYCLNMLAIAIELAKHNP"
BASE COUNT          756 a          768 c          754 g          512 t
ORIGIN      
        1 tgcagcgaat caaatatcag gcaactatcc gctaatatat caaaatttga tcaccgggca
       61 gatgctcgta cgcaccactg ccatcccggc gcgagaccgt ctcattttaa ttattagtgc
      121 acatagatga acttgtaaat agacggatcc gtgagcgctt gttattaata gaaggtcttg
      181 aaagagaagt ctcccatgag cccgtataat aaagagcagt cccgtcccgc tgtttagtca
      241 tgcgccgtgc gcggctccat atcctgtgta tatcccgatg ctcgctttcg gccggaccgg
      301 aattcacgcg gcactcgggg ggcggcaggg ttaaccaatc atttacctaa tcggctttcg
      361 ccgggccttg ccgcgtaact cgtccgtcat aatattaatt tcacgttaat ctgaaaattt
      421 agagatgaca ccgggaaaat attgatatat ccttgtatga attagtaaat ttatacatcc
      481 atatcgcggg taaaggagaa ccatgatggc aaacaaaaag gaagcggccc cgaagaagac
      541 cgcagtcaaa aaggtggcga ccccaaagac cacgagtaaa aaatcctcac cggcgaagga
      601 atccgttcag gataaaatcg caagggtagc gtacgagctt tacgaaaaaa gcggatacgt
      661 gcacggaaga gacgtcgaac actggatcga ggccgaaagg atagtcctgg gaaaaaagaa
      721 gaagtagatt tcgactttct caataattct acggaacgct cttcatcatc aactatatta
      781 gtacctccgc gaatactata aggcttcggc ctcagtcaat aatcccctat tcatatccct
      841 ctgtccgcgc tgtataatag ctttaaaaat aatagaagct gtaaacactt tcagccccgc
      901 acggcgtctc aaaaaccagg ctgcgacgac gacgaaataa ccagggagga gttgagggct
      961 ttagaaaccg ggcgaaccgg agcatagacg ctcccgggac cgctccctac gacaggcccg
     1021 cgcgatcatg aatacaggga ccaagctcac cgcagaagag aagagactga gactggacac
     1081 cgagaaaaag gcttactgga ggagatgggg gccttacctg agcgagaggc agtgggggac
     1141 cgtgagggag gactacagcc cgggaggcac ggcgtgggaa tacctgcccc atgaccacgc
     1201 gcgctcgcgg gcgtacaggt ggggcgagga cggcctcgca ggcatctcgg acaaccacca
     1261 gaggctctgc ttctcagtcg cgctctggaa cgggcacgac ccgatactga aagagaggct
     1321 cttcggtctc accggcgacg agggcaacca cggggaggac gtcaaggaat attactacta
     1381 cctcgacaac acgccctccc acgcctacat gaagtgcctc tacaaatacc cgcaaaaggc
     1441 ctacccctat gacgacctcg tcgaggtaaa taaacagagg agccgccatg agcccgaata
     1501 cgagctcata gatacgggag tgttcgacga cgaacgctac tgggacgtat tcgtcgagta
     1561 cgcgaagaag accccggaag acatacacat cctgatcacc gtcgccaaca ggggaccctc
     1621 ggaagcagtg ctgcacctcc tccccacgct ctggttcaga aacacgtggt cgtggaaggg
     1681 aggggaaaag ccctctctcc gctacgcgga gtcggaggat aaattgaagg tgattgaagc
     1741 ctcccacccg accctcggga acaggtggct ctactgtgat aacccgggcg gtctgctatt
     1801 cacggacaac gaaaccaact tcgagaggat atacggctcc ccgaacgata cccctttcgc
     1861 caaggacggt atcaacgact atatagtcgg gggcagagag agcgccgtca acggcaaatg
     1921 cacggggaca aaggtatcgg cccattacac gctcacggta ccgcccggaa aatccgtgac
     1981 ggtgaagctc agactctcgg acacgataaa cctgatagac ccgttcggaa agtcgtacga
     2041 cacggtcttc gcaatgcgca aaaaagaagc ggacgagttc tacaggaggg tatgccctta
     2101 cacgctcccg gaggacttgg cctccataca gcgccaggcg ttcgcgggga tgctgtggac
     2161 gaagcagttc tattactacg tcgtccagga atggctcgac ggagacccgc tcgacccccc
     2221 tccccccgaa tcccgcaagc aggggaggaa ccacaactgg attcacgtct ataacgacga
     2281 catactgtcc atgcccgaca aatgggagta tccatggttc gccgcgtggg acctcgcatt
     2341 ccatacagtt acgttttcgc tcatagaccc cgatttcgcg aagcgccagc tcatgctcct
     2401 cacgagggaa tggtacatgc acccgaacgg ccagataccg gcctacgaat gggcgttcag
     2461 cgacgtcaac ccgcccgtac acgcgtgggc cgcgtggagg gtatataaca tagagcacaa
     2521 gatgtacggg cgcagggaca tccacttcct cgaaagggtg ttccagaaac ttcttctcaa
     2581 cttcacgtgg tgggtcaaca ggaaggacgc cgagggaaag aacgtattcg agggcgggtt
     2641 cctcgggctc gacaacatag gcgtcttcga caggaacgcg aagctcccgt ctggagtgat
     2701 actcgagcag gcggacggca cgagctggat gggcatgtac tgtctcaaca tgctcgcgat
     2761 agccatcgag ctcgcgaagc acaacccgag
//
LOCUS       DASSJH010000040        11594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_873258, whole genome shotgun sequence.
ACCESSION   DASSJH010000040 DASSJH010000000
VERSION     DASSJH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11594
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873258"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFJ67_00835"
     CDS             complement(<1..299)
                     /locus_tag="VFJ67_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7288952.1"
                     /translation="MNSEKPRLIAVLGPTASGKSRLAMELAGRLGAEIVSADSLQLYR
                     HLDIGTAKPSPGERGAIAHHLIDIADPGEEFNAGRFRALAREVIRELHSRGKKVIL"
     gene            complement(387..2468)
                     /gene="pnp"
                     /locus_tag="VFJ67_00840"
     CDS             complement(387..2468)
                     /gene="pnp"
                     /locus_tag="VFJ67_00840"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7288953.1"
                     /translation="MFDKHYALETGSLARQANGAVFARYEDTAVLATVVALDEKSEGE
                     DFLPLTINYQEKSAAAGKIPGGYFKREGRPNEKEVLTSRLIDRPIRPLIPSDFTYQTQ
                     IIITVFSADADYDPDVLSITASSAALMVSDVPFDGPLAAVRVGRVGGSFICNPTKKDL
                     AESDMNIVVAGTKEAIVMVEGGAKEVPEAEIVEALMFAHNSIQEFIKLQEDLVSGLGV
                     EKRVMDPPVESPLESEVLAFAGQKLRDTLISASKTERKQRIKSIHAETEEHIKSLHPD
                     EEINVSGPFEKILKDLVRELIVKDKMRLDGRGYADVRNIYGEVGFLSRAHGSALFTRG
                     ETQAAVTATLGTTYDEQRIDSLEGDITKTFMLHYNFPPYSVGEVSFRLGPGRREIGHG
                     ALAERSIIPVLPDKDKFPYTIRVVSDVLESNGSSSMATVCGATLSLMDAGVPISAPVG
                     GIAMGLIKEGDHFVILTDILGDEDHLGDMDFKVAGTKTGVTALQMDIKVSGVTKEILT
                     AALEQAKTARLTVLDKMSGILEAPREDISTYAPRILTMQISQDKIKDVIGSGGKTIKK
                     IVEITGVQIDIDDSGRVNIASPDREACDKAVRIIQGIVEEIEVGKIYRGTVKRILDFG
                     AIVELGYGKDGLVHISELAPTRVKNVTDILKEKDEVLVKCIGKEHDGKIRLSRKQALD
                     ENIENYTNSAI"
     gene            complement(2522..2791)
                     /gene="rpsO"
                     /locus_tag="VFJ67_00845"
     CDS             complement(2522..2791)
                     /gene="rpsO"
                     /locus_tag="VFJ67_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7288954.1"
                     /translation="MSIEKEEKTQIISDYAMHQNDVGSPEVQVAILTRRIKDLSLHTE
                     KSPKDHHSRRGLVSLVAKRRRLLNYIRKNKPETYPDLIQKLGLRK"
     gene            complement(2840..3742)
                     /gene="truB"
                     /locus_tag="VFJ67_00850"
     CDS             complement(2840..3742)
                     /gene="truB"
                     /locus_tag="VFJ67_00850"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET7288955.1"
                     /translation="MLVLDKPRGITSQAAVNTVNRLLKARRAGHTGTLDPFATGVLPI
                     CVNSATKIIPFMVSGNKKYEAEMRLGVKTDTLDLTGRVTGEKEIGIINNSELLGVFSK
                     FRGKINQVPPMYSALKKDGVRLYEYARRGIDVPRAERTVIIDALELLDFNPPFVRFMV
                     ECSRGTYIRVLASDIADELGCGGHLTELRRIESDGFSIDDAVTIEDIRNGSVSLTPLD
                     HALSHMRRVEIGREAACNIREGKQITKSSLDRLGISRFESGERVMICEGDTLVAISEA
                     VMDSAETEDATGGEVVFRLLRVFN"
     gene            complement(3748..4734)
                     /locus_tag="VFJ67_00855"
     CDS             complement(3748..4734)
                     /locus_tag="VFJ67_00855"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET7288956.1"
                     /translation="MGSELETILGLIREAKRVLISSHENPDGDAIGSMLGLGLGLEKI
                     GKEVVFYNRDGVPEILRFLPRSDRVGNSINDIKGTFDLVFAVDCTGTNRAGAEFEDFL
                     KSGRGGKVVIMDHHQTNGPSADYQLLDPLSSSTGIIVYSVLKRLPVEIDSDIAKNLYT
                     TIIGDTGSFRYSNTNPETFRVAAELVERGADPAEISEALFESEPLRKLKLIGLVLPTL
                     EVSGDKRVASVYVDRDMYLQSGACREDSEGIVNIPRSIKGIEVAALFREEIRKDGQSW
                     KVSLRSKGDVDVAKIAEGFGGGGHKKAAGCSIGGSLDDVKKRMFSAIEEALH"
     gene            complement(4724..5074)
                     /gene="rbfA"
                     /locus_tag="VFJ67_00860"
     CDS             complement(4724..5074)
                     /gene="rbfA"
                     /locus_tag="VFJ67_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET7288957.1"
                     /translation="MAHRRSERVSDEIMREVSGMIVRGEIKDPRVSGVFVTGVKITEN
                     LSMAEVFFTVLGGEAERSAALEGLEHSKGFIRSRLAKKIRMKKIPDIKFSFDQALETG
                     YRVDEILRGITRGQ"
     gene            complement(5090..5380)
                     /locus_tag="VFJ67_00865"
     CDS             complement(5090..5380)
                     /locus_tag="VFJ67_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET7288958.1"
                     /translation="MVIGILRIDLYMNGNRSLKAKRQTLKSLIQRVKSRFNNVSISEV
                     GSHDLWQKATIGVSIVGSETRVVNSVLDRITLFIESTGIVEMGGRELEIIHF"
     gene            complement(5392..8019)
                     /gene="infB"
                     /locus_tag="VFJ67_00870"
     CDS             complement(5392..8019)
                     /gene="infB"
                     /locus_tag="VFJ67_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET7288959.1"
                     /translation="MSNVRVYELSKDLNKSNNEILNVAKGLGISVKSHASSITEEEAQ
                     KIRDRVASSSGGGSGAPQKSAEEEKEKVRVFRSEKGAETVERRQGERVVLRRKKKMEE
                     PAEEEVRMQPAEIAAAEETAGLSKETVIEPGEGASAERTLETLEDSLLEEPSAEAEAE
                     FMESVSSGEEAEEAETGQTAVAGSQEKTEKEDADEAAKKVKKKGRRVKPKREEIIDED
                     TLEELRKAFRTKLPGRKREYLVDDRRARSRTTASTAHREKSFDGKSRDLFRVRDSASE
                     TGEAARVIPFPAKPARKTIKIGESVNLGELAKMMSLKAGDVIKKLITMGVRATLNQTL
                     DHETATLVAEEFGFDVSVDIFEEKDILLETYPEESERVLTPRPPVVTVMGHVDHGKTT
                     LLDAIRETNVVAGEAGGITQHIGAYAVDVDGRKVSFVDTPGHEAFTAMRARGAKVTDI
                     VILVVAADDGVMPQTVEAVNHARAAQVPIIVAVNKIDKPEANTERIKRQLSEIGLLSE
                     EWGGDTLFAEVSAKQKIGLRELLELVILQADILELKADVHARANGFVIEAKLDKGRGP
                     VATVIIKEGTLRIGDYLVSGTTSGKVRALIDDKGLRVSEAGPSMPVEIMGLSGVPEAG
                     DLFYVVKDERAAKDVVAYREMKQRESASAKERKPTLENLFESLEKEEAKELALIIKAD
                     TQGSVEAVTESISKLSTEKCQVKIVHTGVGAITETDVALASASNAIIVGFNVRPDVKS
                     LETAEREGVSLELHTIIYNLIDRVRSAMEGLLEPIVKEKVTGHAEVKETFNISRIGTI
                     AGCLVTDGKVSRDNSVRVLRDGVVIFEGKLASLKRFKDDVREVNTGYECGIGVERFND
                     IKVGDIIEFYTHEHFKQEL"
     gene            complement(8221..9546)
                     /gene="nusA"
                     /locus_tag="VFJ67_00875"
     CDS             complement(8221..9546)
                     /gene="nusA"
                     /locus_tag="VFJ67_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET7288960.1"
                     /translation="MATMTELSRVMDSVCKDKGIEKGEIVSAVEEAVLSAAQKLFRMQ
                     EKEKELEVHFNEDDGDVELFEFKTVVEEVADPDMEITLGEAVKLDPEAEVGDEIGVKI
                     NPEFTRIAIQNAKQKILQSIKEAEGKVIYEEFKNRKGELISGIVRRVERKNIIVDLGR
                     TEAILPPEQQVPREYYKPKERIRAILYEIKETKRGPQLILSRSHRDFVRKLFESEVPE
                     IGDQIVSIKAIARDPGGRTKIAVASNDTDVDPVGACVGMRGARVQNVIQELKGEKIDI
                     VPWSSDAARFACNALSPARVSKVIIDDDNKSMEIIVNDDQLSLAIGRRGQNVRLASQL
                     CEWKIDIKTESQVKREQQDVVTLLMSLPNVKEVTANLLYGEGFHKLEDIAFADPETLV
                     KSAGLKNEEDALKLQTAARIALKDKLEQMSFTEGEAPQAEVENAAEQEQ"
     gene            complement(9558..10025)
                     /gene="rimP"
                     /locus_tag="VFJ67_00880"
     CDS             complement(9558..10025)
                     /gene="rimP"
                     /locus_tag="VFJ67_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET7288961.1"
                     /translation="MVSREKDILFNVRELLEPILAGKNLELFDVEFKGQGKNGVLRVF
                     IDREEGVTIDDCALVSRELGTLLDIHDMIPGSYTLEVSSPGLTRPLRKPEDFSRFKGK
                     KVKIKTNKDIEKRKSFIGKLLDFKDGLVTVETEGGSFSIPYADIEKANLELDF"
     gene            complement(10295..>11594)
                     /locus_tag="VFJ67_00885"
     CDS             complement(10295..>11594)
                     /locus_tag="VFJ67_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HET7288962.1"
                     /translation="GVALDEFDGVSWRSGGLGYRTYKPDSEGRFTVGSTAARDNDMLE
                     QEIITEPIDTDVLFSANIPAAFSSVPGGRVSELNDSYSLRDRMSYRIKYRALSNIHTP
                     SPRELRSDRSPDNEDMETYLRLPDLSGRVRDLALEITSSDDNRYDKALSVKRYLLTNY
                     SYTRTLGRGASEFPLEEFLFGGKEGHCEYFATAMVILLREAGVPSRIVNGFVGGTPNE
                     HGNFYLVRESDAHSWVEVYFPGHGWVSFDPTPEGRDNPDGGFLAVAASYVDYLRFRWN
                     RYVIDFSERDQMRLLTEARDKWRWNNRALFRVGGFKSLLDERAVGAAAVVLLFLWLAA
                     SRPDIGSLLRLRRRSPGGEASALYSEALRFLSRKGFPKPGSMTAGEFSDSLIENSCPG
                     ASVMKTLTGKYLSLRFGGSEESEELKTLGRLLGELKRTKA"
BASE COUNT         2139 a         3548 c         2905 g         3002 t
ORIGIN      
        1 agaattactt tcttgcccct cgaatgaagc tcccggatga cttcccgggc gagcgccctg
       61 aacctgccgg cgttgaattc ctcgccgggg tcggcgatat cgatgaggtg gtgagctatc
      121 gccccccgct ctccgggtga aggctttgca gtgcctatgt cgaggtgtct atatagctgg
      181 agggagtcgg cgctcacgat ctcggcgccg agccgcccgg caagctccat cgcgagcctg
      241 cttttgcccg aagcggtcgg tccgagaact gcgatgagtc tgggtttttc cgagttcact
      301 tttgacgtgt gatgaagagg atctagatct gggaaagaaa atacgtccgc cggcctcaaa
      361 tacggaccgg cgaactattc tctatatcat atcgcgctgt tggtataatt ctctatattc
      421 tcgtcgaggg cctgctttct gctgagtctt atctttccgt cgtgctcctt gccgatgcac
      481 tttacgagga cctcgtcctt ttccttgagg atgtccgtaa cgttcttgac cctcgtcggg
      541 gcgagctccg atatatgcac gaggccgtcc tttccgtagc ctaactccac tatggctccg
      601 aaatcgagaa tcctctttac ggtcccgcgg tatatcttgc cgacttcgat ctcctcgact
      661 atgccctgta taatcctgac agccttgtcg caggcttccc tgtcgggcga ggctatgttc
      721 acccttcccg aatcgtctat atctatctga acgcccgtta tctcgactat cttctttatc
      781 gtcttgccgc ccgagcctat aacgtccttt atcttgtcct ggcttatctg catcgtgagt
      841 attctcggcg cgtaggtcga tatgtcctct ctcggagcct cgaggatacc gctcatttta
      901 tcgagtaccg tgagacgggc cgtcttggcc tgctcgagcg ccgccgtgag aatctctttc
      961 gtcactccgc tgacctttat atccatctgg agcgcggtca cgcctgtctt agttcctgcg
     1021 accttgaagt ccatgtcgcc caggtggtct tcgtccccga ggatgtcggt gagtatcacg
     1081 aaatggtccc cttcctttat aagtcccatc gctatgccgc ccacgggagc ggatatcggc
     1141 acgcccgcgt ccattaacga gagcgtagcg ccgcatacgg ttgccatcga tgaagagccg
     1201 ttcgattcca gaacgtccga taccacacgt atcgtgtagg ggaacttgtc cttatccggc
     1261 agaaccggga taatggacct ctccgcgagg gccccgtgtc ctatctccct ccgtcccggc
     1321 ccgagcctga agctcacctc gcccacgcta taaggcggga aattgtagtg gagcatgaag
     1381 gtcttggtga tgtctccttc gagggagtct atcctctgct cgtcgtaagt cgttccgagc
     1441 gtggccgtga cagccgcctg cgtctctccc ctcgtaaaca aggccgagcc gtgcgccctg
     1501 gacaggaacc cgacttcgcc gtatatgttc cttacatcag cgtaacccct gccgtcgagc
     1561 ctcatcttgt ccttaactat gagctccctt accaggtcct tcagtatctt ctcgaacgga
     1621 cccgatacat tgatttcttc gtccggatgt agggacttga tatgctcttc cgtttcggcg
     1681 tgaatgctct ttatccgctg ttttctctcc gtcttggacg cggagataag cgtgtctctc
     1741 aatttctgcc cggcaaaagc gagtacctcc gattcgaggg gactctctac cgggggatcc
     1801 attacccttt tctcgacacc tagtcccgat acgaggtctt cctggagctt tatgaattcc
     1861 tgaatgctgt tgtgggcgaa catgagcgct tcgactattt cggcctcagg gacttctttg
     1921 gcgccgcctt ctaccattac gatcgcttcc ttcgtgccgg cgaccacgat gttcatgtca
     1981 ctttcggcca ggtccttctt cgtagggttg catataaacg agcccccgac cctgcccacc
     2041 ctgaccgccg ccagggggcc gtcgaagggg acgtccgaga ccattagcgc cgccgaagac
     2101 gccgtgatcg aaagaacgtc cgggtcgtaa tcggcatcgg ccgagaatac ggtaatgata
     2161 atctgtgtct ggtatgtaaa atcgcttggg ataagcggcc ttatcggtct gtctataagc
     2221 ctcgaagtga ggacttcctt ttcgttgggt ctcccttccc gcttgaaata tccgcctgga
     2281 atctttccgg ccgctgcgga tttctcctga tagttgatgg ttaacgggag aaagtcctcg
     2341 ccttcggatt tttcgtcgag tgcgacgacc gtagccagaa ccgccgtgtc ttcataacgg
     2401 gcgaatacgg ccccgttcgc ctgtctggcg agtgatccgg tctcgagcgc gtaatgtttg
     2461 tcgaatatac gggcgtcgac cttttttggt gttgtagtca aaactggtcc tcctttgaac
     2521 cctactttct aagtccgagt ttctgaatga gatccggata tgtctccggt ttgtttttcc
     2581 tgatgtaatt aaggagtctt ctccttttcg cgacgagcga gacgagtccc ctcctcgaat
     2641 gatgatcctt gggagacttc tccgtgtgga gcgaaaggtc ctttatccta cgcgtgagta
     2701 tcgccacctg gacctcgggc gagcctacgt cgttctggtg catcgcgtag tcgcttatta
     2761 tctgtgtttt ttcttccttc tcaattgaca ttaactgccc tccttaaccc gctgtttctt
     2821 ttttataaat aatcgacctc taattaaaaa ccctgagcag cctgaatacg acctcgccgc
     2881 ccgtcgcgtc ttccgtttcc gccgaatcca tcacggcctc gctgatcgcc acgagcgtat
     2941 cgccctcgca aatcattacc ctctccccag attcgaaccg ggagatgccg agcctgtcga
     3001 gactactctt ggttatctgc tttccttccc ttatattgca cgcggcttcc cgtccgatct
     3061 caacgcgcct catgtgggag agcgcatgat cgagcggggt cagcgagacc gagccgttcc
     3121 ttatatcctc aatggttacg gcgtcgtcaa tgctgaaacc gtcgctttcg attctcctta
     3181 actcggtaag gtgtccgccg cacccgagct cgtcggcgat gtcggaagca agcaccctta
     3241 tatacgtacc gcgggaacat tctaccataa atctgacgaa tgggggattg aaatccagta
     3301 attcgagcgc atcaatgatt acagttcttt cggccctcgg gacatctata ccccgcctcg
     3361 catactcata cagcctgacc ccgtccttct tcagggcgga atacatgggc ggaacctgat
     3421 taattttacc ccgaaatttg gaaaatacac ctaatagctc ggaattattg ataataccaa
     3481 tttccttctc tccggtgacc ctgccagtga gatcgagcgt gtcggtcttg accccgagcc
     3541 tcatttccgc ctcgtatttt ttattgccgc tcaccatgaa gggtattatc ttcgtcgcgc
     3601 tgttgacgca tatgggcaga accccggtcg caaagggatc gagcgtcccg gtgtgccccg
     3661 cccgcctggc cttgagcagc ctgttcaccg tattgaccgc cgcctgcgag gtaatgcccc
     3721 tcggtttgtc gagaaccagc acgccgttca gtgaagagcc tcctcaatcg cgctgaacat
     3781 cctcttcttc acgtcatcga gactgcctcc gatagagcat ccggcggctt ttttatgacc
     3841 cccgcctccg aacccctcgg ctatcttcgc cacgtccacg tctcctttgg acctgaggct
     3901 gactttccag gactggccgt cctttctgat ttcctccctg aacagagcgg ccacctcaat
     3961 gccctttatg ctcctcggaa tattcactat gccctcgctg tcttccctgc aggcgcctga
     4021 ctgcaggtac atatccctgt cgacgtaaac ggaggctacc cttttatccc cggacacctc
     4081 gagcgtcgga agcacgagcc cgatgagctt gagcttcctg agaggctcgc tttcaaataa
     4141 ggcctcggat atctcggccg ggtcggcgcc gcgctcgacg agctcggcag cgaccctgaa
     4201 cgtctccggg ttggtgttcg agtatctgaa tgatccggta tcgcctatta tagtcgtata
     4261 aaggttcttc gctatgtcgc tgtctatctc tacaggcaga cgcttgagga ccgaatagac
     4321 tatgatcccc gtggatgaag aaagcgggtc gaggagctgg tagtcggccg aggggccgtt
     4381 tgtctggtgg tggtccatta ttacgacctt cccgcccctg ccggacttga ggaaatcctc
     4441 gaattccgcc cctgccctgt tggtccccgt gcagtcgacc gcgaatacga gatcgaaagt
     4501 cccttttata tcgtttatgg aattgcctac cctgtccgac cgggggagga aacggaggat
     4561 ctccggcacg ccgtccctat tgtagaacac cacttccttg cctatcttct ccaacccgag
     4621 accgagaccc agcattgagc ctatcgcgtc gccgtccggg ttttcatggg aagagatcag
     4681 cacgcgcttc gcctctctta taagtccgag tatcgtttcc aattcactgc ccacgcgtaa
     4741 tccctctcaa tatctcatcg accctgtagc ccgtctcgag cgcctggtcg aagctgaatt
     4801 taatgtccgg tatcttcttc atcctgattt tcttcgcgag tctgctgcga atgaatccct
     4861 tcgagtgctc gagtccttcg agcgcggcag acctttcggc ctcccctccg agaacggtga
     4921 agaagacctc agccatgctg aggttctcgg ttattttcac gcccgttacg aacactccgg
     4981 agacgcgcgg gtcttttatc tcacccctca ctatcatccc ggacacttcc ctcattatct
     5041 cgtccgaaac cctctctgac cttctatgcg ccaaccccgg acctttctcc taaaaatgta
     5101 ttatttcgag ctccctgccg cccatttcga cgatcccagt agattcgata aacagcgtta
     5161 tgcggtcgag cacggagttc accacccgtg tctcgctgcc gactatagaa acgccgatag
     5221 tcgccttctg ccagaggtcg tgagatccca cctcggatat ggatacgttg ttgaacctcg
     5281 attttactct ctggataagg cttttcaggg tctgtctctt ggctttgagg gatctgttac
     5341 cgttcatata tagatcaatg cgtagtatgc ctataaccat ctgcccgcgg atcagagctc
     5401 ttgtttgaag tgctcatgag tgtagaactc tataatgtcg ccgaccttta tatcgttgaa
     5461 cctctcgacg cctataccgc actcataacc tgtgtttacc tctctcacgt cgtccttaaa
     5521 ccgcttgagg gaagcgagct tgccctcgaa tataacgact ccgtccctga gaaccctgac
     5581 gctgttgtct ctcgagacct tgccgtccgt gacgaggcat ccggcgatcg tgccgatcct
     5641 cgatatgttg aaggtctcct tgacttccgc gtgtcccgta accttttcct tgacaatagg
     5701 ctcaaggagg ccttccatgg cgctcctgac cctgtctatg agattgtata ttatggtgtg
     5761 gagctcaaga gagacccctt ccctctcggc agtctcgagg gactttacgt cgggtctgac
     5821 gttgaaaccg actattatcg cgttcgacgc gctcgcgagg gcgacgtccg tctccgtaat
     5881 cgcgccaacg cccgtatgta ctatctttac ctggcacttc tcggtgctga gcttgcttat
     5941 cgattcggtt acagcctcca cagatccctg tgtgtccgcc ttgattatga gagcgagctc
     6001 tttcgcctct tctttctcaa gactctcgaa gagattctca agcgtcggct ttctctcttt
     6061 ggccgaggca gattccctct gtttcatctc cctgtaggcg accacgtctt tggcggccct
     6121 ctcgtccttc acgacgtaga agaggtcgcc cgcttcgggc acgcccgaga gacccattat
     6181 ctcgacgggc atggaagggc cggcttcgct cacgcggagc cccttgtcgt cgatgagggc
     6241 tctgaccttg cccgatgtgg tccctgaaac caggtagtcc cctatacgga gagtcccttc
     6301 ctttattatg actgtggcta caggccccct ccccttatcg agcttggcct ctatcacgaa
     6361 gccgttcgcc ctggcatgga cgtcggcctt gagctcgagt atgtccgcct gaagaatcac
     6421 gagctcaagc aactcccgga gtccgatctt ctgttttgcc gatacttcgg caaagagggt
     6481 atctccgccc cattcctccg ataaaagacc tatctcggag agctgtctct tgattctttc
     6541 cgtattcgct tcgggtttgt ctattttatt tacggcgact attatcggca cctgggccgc
     6601 ccgcgcatgg tttacggcct ctaccgtctg cggcatgacg ccgtcatccg ccgcgacgac
     6661 gagaataact atgtcggtga cctttgcgcc gcgcgccctc atcgccgtga acgcctcgtg
     6721 gccgggagtg tcgacgaaag agactttcct tccgtccacg tccactgcat aagctcctat
     6781 atgctgggta atgccgcccg cctcgcccgc gacaacgttc gtctccctta tcgcgtcaag
     6841 aagcgtcgtc ttcccgtggt cgacgtgccc cattacggtt acaacagggg gcctcggcgt
     6901 gagcaccctc tccgattctt cgggataggt ttcgaggagt atgtccttct cctcgaaaat
     6961 atctacagag acgtcgaacc cgaactcctc ggcgacgaga gtcgccgtct catggtcgag
     7021 agtctggttg agcgtcgccc tcacacccat cgtaatcagc ttctttatca cgtctcccgc
     7081 cttgaggctc atcatcttcg caagctcgcc aagatttacg gattcgccta tcttgattgt
     7141 ttttctggca ggtttggcgg gaaacggaat gaccctcgcg gcctcgcccg tctcggacgc
     7201 gctgtctctt accctgaaca agtccctgga cttgccgtca aacgactttt ccctgtgcgc
     7261 cgtgctcgcc gtagtgcgcg acctcgcgcg cctgtcgtcg accaggtatt ctctctttct
     7321 cccggggagc ttcgtacgaa aggccttcct cagctcctcg agcgtatctt cgtctattat
     7381 ttcttccctt ttcggtttta ccctgcgtcc ctttttctta accttcttgg ccgcttcatc
     7441 ggcatcttcc ttctcggttt tctcttggga ccccgccact gccgtctgac ccgtttcggc
     7501 ctcctcggcc tcttcgccgg aggaaacact ttccataaat tccgcctcgg cctcagccga
     7561 cggctcttca aggagcgagt cctcgagggt ctcaagagtg cgctcagcag aagcgccctc
     7621 tccgggctcg attacagttt ccttcgatag acccgcggtc tcttcggccg ccgctatctc
     7681 agccggctgc atcctgacct cttcttcggc aggctcctcc attttctttt tccttcggag
     7741 caccaccctt tcgccctgcc tgcgctcgac ggtctccgcc cctttttcgg aacggaacac
     7801 cctgaccttt tccttttctt cctcggctga tttctgcggc gcgcccgagc ctccgcccga
     7861 ggaagaagcc accctgtccc ttatcttctg cgcttcctct tccgttatcg agctcgcgtg
     7921 gctcttgacc gatataccga ggcccttggc gacgttgagt atttcgttgt tcgacttgtt
     7981 caaatctttc gagagttcgt agactcttac attggacata tataacacac ctttacaata
     8041 agcacttatt tacaataaat tgataaagtt aagtttatcc agaaaagagt accaaagttt
     8101 acaagctatt gcaaaagcgg gactcttacg gaagcgctgc gccgatgaaa taatcgggtc
     8161 gccgtatatt ttcccggcgg cctcggggca gatgcagacc ggatttgcgc gtgccaggtg
     8221 ctattgctcc tgctccgcag cgttctcgac ctcagcctgg ggagcctcac cttcggtgaa
     8281 ggacatctgc tcgagcttgt ccttgagcgc tatcctcgcc gcggtctgca gcttgagggc
     8341 atcttcctcg tttttgagcc cggccgactt tacgagcgtc tccggatcgg caaaggcgat
     8401 gtcctcgagc ttgtggaatc cctccccgta aagcagattc gccgtcactt ccttcacgtt
     8461 aggcagcgac atgaggagcg taacgacatc ctgctgctcg cgcttgacct gggattcggt
     8521 cttgatatct attttccact cgcatagctg tgaggcgagc ctcacgttct gtcccctcct
     8581 gcctatcgca agggagagct ggtcgtcgtt gactattatc tccatggatt tattgtcgtc
     8641 gtcaattatc accttgctta cccttgccgg ggaaagggcg ttgcatgcga acctcgccgc
     8701 atccgaagac catgggacta tgtctatctt ctcgcccttc aactcctgta tgacgttttg
     8761 taccctggct cccctcatgc ccacgcacgc gcccacgggg tctacgtcgg tgtcgttaga
     8821 cgcgacggcg atcttcgtgc ggcccccggg gtccctggct atggccttga tcgagactat
     8881 ctgatcccct atctcgggca cttcggattc gaagagcttg cggacgaaat cgcggtgcga
     8941 gcgcgagagg ataagctgcg ggcccctctt ggtctccttg atctcgtata gtatggccct
     9001 tatcctttcc ttgggcttgt agtactccct cgggacctgc tgctcgggag ggagaattgc
     9061 ctcggttctg cccaaatcga ctattatgtt ctttctttcc acgcgcctca ctataccgct
     9121 tatgagctcg cctttcctgt tcttgaattc ctcgtatatt accttgcctt cagcctcttt
     9181 aatgctctgg agtatcttct gcttggcgtt ctgtatagct atcctggtaa attccggatt
     9241 tatcttgacg cctatctcgt cgccgacctc ggcctcgggg tcgagcttga cggcctcgcc
     9301 gagggttatc tccatgtcgg ggtcggctac ttcctcgacc acggtcttga actcgaacag
     9361 ctccacatcg ccgtcgtcct cgttgaagtg cacctctagc tccttctcct tctcctgcat
     9421 gcggaagagc ttctgggccg cggagagcac agcttcctcg acggcgctca cgatctcgcc
     9481 cttctctatg cccttgtcct tgcaaacaga atccatgaca cggcttaatt ccgtcattgt
     9541 agccatttgt tgccccctta gaaatccaac tccaggttag ccttctctat gtcggcgtac
     9601 ggtatggaga aactgccccc ttccgtctcg accgtaacga gtccgtcctt gaaatcgagg
     9661 agtttgccta tgaacgattt cctcttttct atatccttat tcgtcttgat cttcactttt
     9721 tttcccttga atctggagaa atcctccggt ttccggagcg gtctcgtgag tcccggagag
     9781 gagacctcaa gcgtatatga gccgggtatc atgtcgtgta tatcgagcag cgtgccgagc
     9841 tccctgctca cgagtgcaca gtcgtctatg gtcacgcctt cttccctgtc tataaaaacc
     9901 ctcagtacac cgttttttcc ctgccccttg aactctacat caaaaagctc aaggttcttg
     9961 ccggcgagga tgggttctaa taactccctg acattaaaca atatatcctt ttccctagaa
    10021 accatcgcgc cccgctctct caattaaaaa agcgggacga acccgctttt gtcataagat
    10081 tttacctcaa taggcatgat agagcaagga gaggcggagg cccgaagggc cggcagaggc
    10141 acgatagaac aggaagaggc gatggcccga agggccggca gaggcatgat agaacaggaa
    10201 gaggcggtgg cccgaagggc cggcagaggc atgatagaac gagcatagct gggcgccgga
    10261 agggcgggga acatgcttag cccggagcca tgaatcaggc tttcgtcctc ttaagctccc
    10321 cgagcagcct gcctagtgtc tttagctctt cgctttcctc cgagccgccg aacctcaagg
    10381 agaggtactt tccggtcagt gtcttcatca ctgaggcccc ggggcaggag ttctcaatga
    10441 gagagtcgga aaactcgccc gccgtcatgg aaccgggctt cgggaacccc ttccgggata
    10501 aaaatctcaa cgcttctgaa tagagtgccg aggcttcccc ccccgggctc cttctcctca
    10561 gccggaggag cgatccgata tcgggtctcg acgccgcgag ccacaggaaa aggaggacca
    10621 cggcggcagc gccgaccgcc ctctcgtcga gcagggattt gaagcccccg acacggaaga
    10681 gcgccctgtt attccagcgc cacttgtccc gggcctccgt taggagcctc atctggtccc
    10741 gctcgctgaa atctatcacg tacctgttcc acctgaaacg gaggtaatcg acgtaggagg
    10801 cggctaccgc cagaaagccg ccgtccgggt tgtcccggcc ctcgggcgtc gggtcgaagg
    10861 agacccaacc gtgccctggg aaatatacct ccacccagga gtgggcgtcg ctctcgcgga
    10921 cgaggtaaaa attcccgtgc tcgttcggcg tcccgcctac gaagccgttc acgatcctcg
    10981 aagggacccc cgcttcccgg agcaggatga ccatggccgt ggcgaagtat tcgcaatgcc
    11041 cctctttccc gccgaagagg aattcttcga gcgggaattc cgaagcgccc cgcccgagcg
    11101 tgcgcgtata ggagtagttc gtgagcaagt accgctttac ggagagggcc ttgtcatacc
    11161 tgttgtcatc cgaagaggtt atctcgaggg cgagatccct tacgcgcccg ctcagatcgg
    11221 gcagccggag ataggtttcc atgtcttcgt tatccgggga cctgtcgctc cggagctctc
    11281 ggggagaggg ggtatggatg tttgataaag ccctgtattt tatcctgtag gacatcctgt
    11341 cccggaggct gtaggaatcg ttcagctcgg agaccctccc gcccggaacg gagctgaagg
    11401 ctgcggggat attcgccgag aaaagcacgt ccgtgtcgat gggctccgtg attatctcct
    11461 gctcgagcat gtcgttgtcg cgagcggcgg tgcttccgac agtgaacctc ccttcggaat
    11521 cgggcttgta cgtccggtag cctaaaccgc ccgaccgcca ggacacgccg tcgaattcgt
    11581 caagcgcgac tccc
//
LOCUS       DASSJH010000041         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_117195, whole genome shotgun sequence.
ACCESSION   DASSJH010000041 DASSJH010000000
VERSION     DASSJH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117195"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFJ67_00890"
     CDS             complement(<1..213)
                     /locus_tag="VFJ67_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010178895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288963.1"
                     /translation="MSDHTFDFLFVGAGILGSAGAYALSKKLGEKGLKAAIGVIDIDM
                     EGEFSSTLKNAGGVRATWRSRANIELC"
     gene            258..1565
                     /locus_tag="VFJ67_00895"
     CDS             258..1565
                     /locus_tag="VFJ67_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET7288964.1"
                     /translation="MKGLAAGNLCYHFYVMGDQSLRWLFVDMNSYFASVEQQMRPGLR
                     GRPTVVVPVMADSTCCIAASYEAKGYGIKTGTKVAEARRLCPDLEIVESRPEIYIETH
                     HKIVSAVTTILPVDKVQSIDEMACRLTGRQTDYENAVSIGRRVKEAIKEKVGEYVKCS
                     LGLAPNRFLAKVATDMEKPDGFTVITKEDLPHKLYSLSLTDLPGIGRRMHARLKSCGV
                     HTVEALCSQSEGRMRDIWGSVLGERFLLMLMGEDVEEPETGRRTVSHSHVLAPKLRTD
                     EGTRAVFVRLIHKAAFRLRRLGYLAGKMVVRIDYLGGETSWKMRADVRKRNDTQTMIE
                     AFSSLWKKRPGRRKSLRASVVFTDLIPASETSFPLLSGERKRDKVANALDKINERWGT
                     NSIYYGAMHGASESAPLRIAFTSIPDIVAEGNKPKIPVQESKK"
     gene            complement(1581..2087)
                     /locus_tag="VFJ67_00900"
     CDS             complement(1581..2087)
                     /locus_tag="VFJ67_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288965.1"
                     /translation="MKKKEEKAAREKQEAPGRLQAGLKSKDVIQLIEALEGVKLDFDH
                     LYYYEHTGLIVPSLRSSRGRGVPKLYSTEDFIVLRWLVSLQKNGIHVSRFREVLSFLK
                     EKMPEVLRQPQNWVLITDGKSVQFFDKVSSRAFDVLKDTGQYLFFFPIGKVADESRKA
                     VKKFGKKK"
     gene            2223..3095
                     /locus_tag="VFJ67_00905"
     CDS             2223..3095
                     /locus_tag="VFJ67_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006105432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HET7288966.1"
                     /translation="MTKGQKLREILSEQGILVLPGVHDCVSAKLAERAGFSAAFTSGF
                     GISGTTLGKPDYGFLTATEMITSVGRITASVGIPVVADIDTGYGNALNVMNTVEQVVT
                     AGAAGVILEDQVWPKKCGHFEGKHVIPMEEHVEKIHAAAEARGESGIVIMGRTDARAP
                     HGLVEAIRRGRAYYEAGADVVFIEAPQSVEELREIAAAFPGVPLFANMVEGGKTPILD
                     AGELGELGFKIAVFPLAALFSATKAIESCFAYLRKHGTTAGYGDMISFRDFEEIIDVP
                     RYREMEKKFGVPTA"
     gene            complement(3092..4528)
                     /locus_tag="VFJ67_00910"
     CDS             complement(3092..4528)
                     /locus_tag="VFJ67_00910"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HET7288967.1"
                     /translation="MRRVIIVIVLLVVLALFGLPYWFGTQTEKVYNDIVSTYSGTGGV
                     SVVEKTYRRGWLNSTARTVFVIKNGDAELIKFEESDTIYHGPFPVQAVMSGRAGLAPV
                     MAVIDSKLVPVPAGDSEFETLIKKLPPANLLTRLSLDGGGITDITVPGIETEQGEKGE
                     KLKWDGLTGDVKFSPEFRDVDTELKSPGLNISGKDSGFTVSGVDLISRLSYVGPEHKY
                     PIGDIDFKVAGIAVRSKKEGSDEYQNFSVTGIELKGNSTESQGDLNSTHSLSFRELEA
                     GSGKYGPGGYELAIRNIDMESWIKIQELLKKNQNGPQTEEQRQAFMTELMKIVPGLIK
                     KSPEIELTKLNIMTSDGSIDGNMKISVDGSGMDNPEIASNPLFLVTAIKADAKVSVTK
                     TLLETIITDYKKEEIADDFRKADDEVPPPDEIAGMAKEEMGDEIKGLMDQGVITESDK
                     SYDLNASYEMGQITLNGQPLDMQSLIGD"
     gene            complement(4680..5309)
                     /locus_tag="VFJ67_00915"
     CDS             complement(4680..5309)
                     /locus_tag="VFJ67_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288968.1"
                     /translation="MKSINFAGISSLALLMIIVSGCSGLSKSYPERSYFMFDVPPVAQ
                     KAAPVKGAIVEVARFNVSPGSEGKEFIYRTGDVSFESDFYNQFFRPPGAMLTETTIRW
                     LGDTGLFQEVLSTVTQAGANYFIEGNVVQLYGDYRSSPKAVMQIQFLLLKYTSDDTYD
                     DNTGIVFANSYTSEIPIAAKTPNDLMMGWNTALGEILNAFLADLNKNVK"
     gene            complement(5306..>5560)
                     /locus_tag="VFJ67_00920"
     CDS             complement(5306..>5560)
                     /locus_tag="VFJ67_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288969.1"
                     /translation="TILKSKEAKETLANVTAITSDLKNTLNRTDRLLSSREHSVKLTL
                     ENIERISEDLREFMETMKKYPSWVLFGNPPPHIEEEEKPQ"
BASE COUNT         1146 a         1507 c         1588 g         1319 t
ORIGIN      
        1 gcagagctct atgttcgcac ggctcctcca cgtcgccctc acgccccctg cgttcttgag
       61 tgttgacgaa aattcccctt ccatgtctat atctattacg ccgatagctg ctttaaggcc
      121 cttttcgccg agctttttgg agagcgcata agcccccgcc gagccaagta tgcctgcgcc
      181 tacgaagagg aagtcgaaag tgtgatcgct catagaaata ttctatcaca cggttcttta
      241 cggggaggtg tgaagaattg aaaggacttg cggcggggaa tttatgttat catttttacg
      301 tgatgggtga tcagtcgctc aggtggcttt tcgtcgatat gaactcgtac ttcgcctcgg
      361 tcgagcagca gatgaggccc gggctgaggg gcaggcctac tgtcgtcgtc cctgttatgg
      421 ccgacagcac gtgctgcata gccgcgagct acgaggcgaa gggatacggg atcaagaccg
      481 gcacgaaagt agccgaggcg cgcaggcttt gcccggacct cgagatcgtg gaatcgaggc
      541 ccgaaatata catagagacg catcataaga tagtctctgc cgtaaccacc atactgcctg
      601 tcgataaggt gcaatcgatc gacgagatgg cgtgccggtt aaccgggagg cagacggatt
      661 acgaaaacgc cgtctccata ggccgccggg tgaaggaggc gattaaagag aaggtcgggg
      721 aatacgtgaa atgctccctg ggtcttgcgc ctaaccgctt tttagccaag gtggcgaccg
      781 atatggagaa acccgacggc tttacggtca taacgaagga agaccttcca cataaattat
      841 attccctctc gctgaccgac ctgccgggca tagggaggag gatgcacgct cgcttgaaga
      901 gctgcggcgt tcatactgtc gaggcccttt gctctcagtc ggaagggaga atgcgggata
      961 tatgggggag tgtgttgggt gagcgctttc tcctcatgct catgggagaa gacgtggagg
     1021 aacccgaaac cggacggagg accgtgagtc attcccacgt gctcgcgccc aagctcagga
     1081 ccgacgaggg gacgcgggcc gtcttcgtaa ggctcattca taaggccgct ttcaggctga
     1141 ggaggctcgg ttatctggcg gggaagatgg tcgtcaggat agactatctc ggcggggaaa
     1201 cgagctggaa aatgagagcg gacgtccgaa agaggaacga tacccagaca atgatcgagg
     1261 cgttctcttc gctctggaaa aagagacccg ggagacggaa atcgctgcgt gcgtccgtcg
     1321 tatttacgga tctgattccg gcaagcgaga ccagttttcc tcttctttcc ggggagagga
     1381 agcgggacaa ggtcgcaaac gccctcgata agataaacga gcggtggggg acgaacagca
     1441 tctattacgg ggcgatgcac ggggcttccg agtctgcgcc tctcaggata gcgttcactt
     1501 ctattccgga tatagtggcc gaagggaata aaccgaaaat tccggtacag gagtcgaaga
     1561 agtaaaggcc ggcccggact ctatttcttc tttccgaatt ttttaaccgc ttttctgctt
     1621 tcgtcggcta ccttgcctat ggggaagaag aagagatact ggcccgtgtc cttgagcacg
     1681 tcgaacgccc tcgaggatac cttgtcgaag aactgcaccg acttgccgtc cgttatgagc
     1741 acccagttct ggggctgcct cagcacctcg ggcatctttt ccttgaggaa cgacagcact
     1801 tcgcggaagc ggctcacgtg tatcccgttt ttctggagcg agacgagcca cctgagcacg
     1861 atgaagtcct cggtcgagta gagcttgggc actcccctgc ccctcgagct cctcaggctc
     1921 ggcactatga gccccgtgtg ctcgtagtaa tagaggtggt cgaagtcgag cttcacgccc
     1981 tcgagcgcct caatgagctg tattacgtct ttactcttta acccggcctg cagccttccc
     2041 ggagcttcct gtttttccct ggcggctttt tcctctttct tcttcacttc gggtgtccga
     2101 gaaaacgatc gagcgggtta gtctaacttc aaaatatcac aaacgtccgc agggcgcaat
     2161 ccttcttgac acccctcccc tgcgtcacta tgtttctagg gaaaaagtca gggagaggga
     2221 cgatgactaa gggtcagaag ctgagggaga tactgtcgga gcaggggata ctggtgcttc
     2281 cgggcgtgca cgactgcgtg agcgcgaagc tcgccgagag ggccgggttc agcgccgcct
     2341 tcacgagcgg cttcggcata tcaggcacga cactcgggaa gcccgattac ggattcctta
     2401 cggcgaccga aatgataacg agcgtcggcc gtatcacggc ttctgtcggt atccccgtcg
     2461 tggccgatat agatacgggg tacgggaacg cgttgaacgt aatgaatacg gtcgagcaag
     2521 ttgtgacggc aggagcagcc ggggtaatac tcgaagacca ggtatggccc aagaagtgcg
     2581 gccacttcga gggtaaacac gtgatcccga tggaggaaca cgttgagaaa atccacgctg
     2641 cggcggaggc gcggggggaa agcgggatcg tgatcatggg ccgcacggac gcgagggccc
     2701 cccacggtct cgtggaggcg attaggaggg ggagagccta ttacgaggcc ggggccgacg
     2761 tcgtattcat agaggctccc caatcggtcg aggagctgag ggagatcgct gcggcctttc
     2821 ccggagtgcc gctcttcgcc aacatggtcg agggcggaaa gacccctatc ctcgacgccg
     2881 gcgagctggg tgagctcgga ttcaagatag cggtgttccc gctcgccgca ttgttctcgg
     2941 cgacgaaggc gatagagtcc tgtttcgcat atctgaggaa gcacggcact acggccgggt
     3001 acggggacat gatctcgttc agggacttcg aggagataat agacgttccc agatacaggg
     3061 agatggagaa gaagttcggg gtcccgaccg cttaatcgcc gatgagcgac tgcatgtcga
     3121 gaggctgccc gttgagcgtt atctggccca tctcgtaact cgcattgaga tcgtagctct
     3181 tgtcgctctc cgttatcacg ccctggtcca tgagtccctt tatttcgtca cccatttcct
     3241 ctttcgccat tccggcgatc tcatccgggg gaggcacttc gtcgtcggcc tttctgaagt
     3301 cgtccgcgat ctcttccttc ttgtagtcgg tgatgatggt ttcaaggagt gttttcgtta
     3361 cggatacctt cgcgtccgcc tttatcgccg tgaccaggaa gagagggttc gacgctattt
     3421 cggggttatc catgccgctg ccgtctacgg agattttcat gttaccgtct atgctcccgt
     3481 cggacgtcat gatgtttaat tttgtaagct ctatctcagg ggatttcttt atcagaccag
     3541 gcacgatctt catgagctcc gtcatgaacg cctgtctctg ctcctccgtc tgaggcccgt
     3601 tctgattctt tttgaggagc tcctggattt ttatccacga ttccatgtct atgttcctta
     3661 tggcgagctc gtatccgccg gggccgtact taccgctccc cgcttcgagc tccctgaagc
     3721 tgagactgtg cgtgctgttg aggtcgccct gcgactcggt cgaattgcct ttgagctcga
     3781 tgccggtaac cgagaaattc tggtactcgt cgctcccttc cttctttgac cggaccgcta
     3841 ttcccgcaac cttgaagtcg atgtccccga tcgggtattt atgttccggc ccgacgtaac
     3901 tgagcctcga aatcaggtcc actcctgata cggtaaagcc gctgtccttt ccgctgatgt
     3961 tgagcccggg agattttaat tccgtgtcca cgtccctgaa ctcggggctg aatttaacgt
     4021 cccccgtgag cccgtcccac ttgagcttct ctcccttctc cccctgctcc gtctctatgc
     4081 ccggcactgt gatgtccgtt atccctccac cgtctaaaga gagcctggta aggaggttcg
     4141 cgggcgggag tttctttata agcgtctcga actcggaatc cccggccggt acagggacga
     4201 gcttcgaatc tatgaccgcc atcaccggcg ccaggcccgc gcggccgctc atgacggcct
     4261 gaacggggaa aggtccgtgg taaatcgtat cgctctcctc gaatttgata agctcggcgt
     4321 cgccgttctt tatgacgaac acggtcctgg ccgtggagtt cagccacccc ctccggtagg
     4381 tcttctccac aaccgatacg ccgcccgttc cggaataggt gctgactatg tcgttataga
     4441 ccttctccgt ctgggtaccg aaccagtagg gcagtccgaa aagggcgagg acgaccagaa
     4501 gcactataac gattatgacg cggcgcattt ttccccccta tttgttaaag tgtcttataa
     4561 ttatatttct tccggcatcg agaaatcaac accgcggtgt aattaccgat taatccccgt
     4621 tggaccgcca ctaggtgcgg attccggttc tcccggttcc ccgataagcc gtgtaacgcc
     4681 tatttaacgt ttttgttaag gtccgcgaga aacgcgttca atatttcacc gagcgcggta
     4741 ttccagccca tcataagatc gttgggcgtc ttagcggcga tcggtatctc cgatgtatag
     4801 gaattggcga atacgatccc ggtattgtca tcataggtgt cgtcgctcgt gtatttcagg
     4861 agcaggaatt gtatctgcat cacggccttg ggcgaggacc tgtaatcgcc gtaaagctga
     4921 actacattcc cctcgatgaa ataattggcg cctgcctgag taaccgtgct cagtacctcc
     4981 tggaaaagcc ccgtgtcccc gagccagcgt atcgtcgttt cggtaagcat agcgccgggc
     5041 ggtctgaaga actggttgta gaagtcggat tcaaagctca cgtctccggt cctgtagatg
     5101 aactccttgc cttcgctgcc cggggagacg ttgaaccggg cgacttcgac gatcgctcct
     5161 tttaccggcg cggctttttg cgccacgggc gggacatcga acataaagta agacctttcc
     5221 ggatagcttt tactgaggcc ggagcagccc gagacgatga tcattaataa tgctaacgaa
     5281 gagattccgg caaagttaat tgatttcatt gaggtttctc ctcctcttcg atgtgcggcg
     5341 gcgggttccc gaaaaggacc catgacggat acttcttcat tgtctccata aactccctca
     5401 ggtcttccga aatgcgctcg atattctcga gagtcagctt cacgctgtgc tcccggctcg
     5461 agagcaggcg gtcggtccgg ttgagcgtgt ttttgagatc ggaggtgatg gcagtgacgt
     5521 tggcaagggt ctctttcgct tctttgcttt tgagtatcgt
//
LOCUS       DASSJH010000042         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1198434, whole genome shotgun sequence.
ACCESSION   DASSJH010000042 DASSJH010000000
VERSION     DASSJH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198434"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFJ67_00925"
     CDS             complement(<1..472)
                     /locus_tag="VFJ67_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016514734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7288970.1"
                     /translation="MHSQEAKTNGRSPEWKRGFGDKLSRILGKDGVIYAEDELIAYDC
                     DALTGYRIKPLFVVLPKTADEVSRVLRLCYEEGVPFVPRGAGTGLSGGALPTEEGIVI
                     ALSRMNRIIEVDIPNERVVVEPGVTNLSVTGAVSAYGYYYAPDPSSQIACTIGGN"
     gene            618..1046
                     /gene="rplM"
                     /locus_tag="VFJ67_00930"
     CDS             618..1046
                     /gene="rplM"
                     /locus_tag="VFJ67_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7288971.1"
                     /translation="MKTYMAKSEGVEKKWFVIDGEGKAVGRVSSKIARILRGKEKPQF
                     TPHADIGDFVIVINADKLKFTGNKWEDKRYYWHTGHPGGIKSITAGDLADRKPGEILK
                     KAVWGMLPKNRWQKKLITRLKIYTGNEHPHSPQKPETLEV"
     gene            1056..1448
                     /gene="rpsI"
                     /locus_tag="VFJ67_00935"
     CDS             1056..1448
                     /gene="rpsI"
                     /locus_tag="VFJ67_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7288972.1"
                     /translation="MAEVNYYGTGRRKTSTARVWLRAGDGKITINDRDPEQYFPRGYW
                     KVHALEPLTTTQTLGKFDVIVDVKGGGLTGQAGAVRHGIARALLQADEANRKPLKSAG
                     QLSRDSRMVERKKYGLKKARRGQQFSKR"
     gene            1505..2809
                     /locus_tag="VFJ67_00940"
     CDS             1505..2809
                     /locus_tag="VFJ67_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET7288973.1"
                     /translation="MAPPEGKLGVLIPGIGGAVSTTFIAGVEAIKKGLGKPIGSLTQL
                     GRIRLGKRTEKKSPLIKEFLPLAELGDIVFGGWDIFPDNCYEAALKAGVLDKDLIGEL
                     KDTLKKLKPMPAVFSRKYVKNLNGKHLKTGKTKMDLAEQLIEDIKRFKKESGASRLVM
                     VWCGSTEIFIRPSAVHATMSSFEKGLRENHPEIAPSMIYAYAALKSGVPYANGAPNLS
                     VDVPALTDFAKKKKVPIAGKDFKTGQTLMKTILAPGLKSRMIGLHGWFSTNILGNRDG
                     EVLDDPHSFKTKEESKLGVLEYMFQPDLHPDLYSNYYHKVRINYYPPRGDAKEAWDNI
                     DIFGWLGYPMQIKVNFLCRDSILAAPIVLDLVLFLDLARRAGFDGIQEWLSFYFKSPM
                     YGKGLYPEHDLFIQLMKLKNTLRYMKGEELITHLGLDYYNLM"
     gene            complement(2877..>3104)
                     /locus_tag="VFJ67_00945"
     CDS             complement(2877..>3104)
                     /locus_tag="VFJ67_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288974.1"
                     /translation="LVEVKEYAVDMKREAWEYISSFQQYAAGALRGYPVDAAVEAMKE
                     AVQPALEQHGYRDAEGNLYITRKWLAVSTEA"
BASE COUNT          796 a          849 c          874 g          585 t
ORIGIN      
        1 cgttcccgcc gatagtgcag gcgatctgac tcgaaggatc gggggcatag tagtacccgt
       61 atgcgctcac ggcgcccgtg accgagaggt tcgtaacccc cggctcgacg actacacgct
      121 cgttgggaat atccacctcg attatcctgt tcatacggga gagcgctatg actatcccct
      181 cttccgtggg cagcgcgcct ccggacagcc cggtccccgc tcccctcggc acgaagggca
      241 cgccctcctc gtagcagagc ctgaggacgc gcgatacctc gtccgcagtc ttcggcagga
      301 cgacaaaaag gggctttatc ctgtaaccgg tcagcgcgtc gcaatcatag gctatgagct
      361 cgtcttcggc gtatatgacc ccgtcctttc ccaggatccg ggagagtttg tcaccgaagc
      421 ccctcttcca ctcgggtgat ctgccgtttg tttttgcctc ttgtgaatgc ataattattt
      481 acaagggaat cattatatca cctaaggacg ccctaaccaa tttcgggcta tttcccccga
      541 ggcgtcccga atccttgaca ttccgcccac agacggtaaa ttgatttgct taactctaat
      601 agttagaggc acataaaatg aagacttaca tggcaaaaag cgaaggcgtt gagaagaaat
      661 ggttcgtgat cgacggggaa ggcaaggctg tcggaagggt gtcttccaag atagccagaa
      721 tactcagagg aaaagagaag cctcagttca cccctcacgc cgacatcggg gatttcgtaa
      781 tcgtcattaa cgccgataag ctaaagttta cgggcaataa gtgggaagat aagagatatt
      841 actggcacac gggacacccg ggcgggatta aatccatcac agccggtgac cttgccgaca
      901 ggaagcccgg agaaatcctg aaaaaggcgg tctggggcat gcttcctaag aacaggtggc
      961 agaaaaaact cattacgaga ctcaagatat ataccgggaa cgagcatccg cattcaccgc
     1021 agaagcccga gaccctggag gtataaaaga ctaaaatggc tgaagttaac tattacggaa
     1081 ccggaagaag aaaaacctcg actgcccgcg tttggctgag ggcgggggac ggaaagataa
     1141 ccattaacga cagagacccc gagcagtact tcccccgcgg atactggaag gtacacgcgc
     1201 tcgagcccct tacgaccacg cagacactcg ggaaatttga cgtcatcgtc gacgtaaagg
     1261 gcggcggtct gacgggccag gcgggcgcgg tcaggcacgg gatcgcgaga gcgctccttc
     1321 aggccgacga agccaacagg aaaccgctca agtcagcggg ccagctgagc agagactcga
     1381 gaatggtcga gaggaagaag tacggcctca agaaagcaag acgcggtcag cagttctcaa
     1441 agaggtaaaa cttactttgc ctgacaacgg gaaaaagaaa cccgcatcca agaaaaagaa
     1501 gaaaatcgct ccgcccgaag ggaaactggg agttctcatt ccgggcatag gcggagcggt
     1561 gagcacgacg ttcatcgcgg gcgtcgaggc catcaagaag ggactcggca agcccatagg
     1621 ctcgctcaca cagctgggcc ggataaggct cggcaaaagg accgagaaaa aatcaccgct
     1681 cataaaggaa ttcctgccgc tcgcggagct cggcgatatt gtcttcgggg gatgggatat
     1741 attccctgac aactgctacg aagccgcgct caaggccggt gtgctcgata aagacctgat
     1801 aggcgagctc aaagacacgc ttaagaaatt aaagccgatg cctgccgtgt tcagcaggaa
     1861 gtacgtcaag aacctgaacg gcaagcacct taaaaccggc aagaccaaga tggaccttgc
     1921 cgagcagctg atcgaagata taaagaggtt caagaaggag agcggggcat cgcgcctcgt
     1981 tatggtctgg tgcggcagca cggagatatt catcaggcct tcagcagttc acgcgacgat
     2041 gtcctctttc gagaagggac tcagggaaaa ccacccggag atcgcgccga gcatgatata
     2101 cgcctacgcc gcgctcaaga gcggggtgcc ctacgcgaac ggcgctccca acctgtcggt
     2161 ggacgttccc gcgctcacag atttcgcgaa gaagaagaaa gtgccgatag cggggaagga
     2221 cttcaagacg ggtcagacgc tgatgaagac catactcgcg ccgggactga agagcaggat
     2281 gatcgggctt cacggctggt tctcgacgaa cattctcggg aacagggacg gagaggttct
     2341 cgacgacccg cattcattta aaaccaagga agagagcaag ctgggagtcc tcgaatacat
     2401 gttccagccc gacctccatc ccgacctcta ctcgaactac tatcacaagg tcaggatcaa
     2461 ctattacccc cccaggggcg acgcgaagga agcgtgggac aatatagaca ttttcggatg
     2521 gctcggctat ccgatgcaga taaaggtcaa cttcctctgc cgggacagca tactggccgc
     2581 gcccatagtg ctcgatctcg tgctgttcct cgacctcgcg agaagggcgg ggttcgacgg
     2641 gatacaggag tggctgtcgt tctacttcaa gagcccgatg tacggcaagg gcctctaccc
     2701 cgagcacgac ctcttcatac agctgatgaa gctcaagaac acgctcaggt acatgaaggg
     2761 cgaggagctc atcacgcacc tgggtctcga ttattacaac ctgatgtaaa ccctatagta
     2821 atcctgccgg ttaatcacgc tgctgaatat ttagatggga aggtactgaa gctttatcag
     2881 gcctctgtgc ttacggcgag ccatttgcgc gtgatgtaga ggttcccctc ggcgtccctg
     2941 taaccgtgct gctcgagagc gggctggact gcctccttca tcgcttcgac ggcggcatcg
     3001 accggatagc ccctgagggc gcctgccgcg tactgctgga aggagcttat gtattcccag
     3061 gcctccctct tcatgtcgac ggcgtactcc tttacctcga cgag
//
LOCUS       DASSJH010000043         3961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_225572, whole genome shotgun sequence.
ACCESSION   DASSJH010000043 DASSJH010000000
VERSION     DASSJH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225572"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..623)
                     /locus_tag="VFJ67_00950"
     CDS             complement(165..623)
                     /locus_tag="VFJ67_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288975.1"
                     /translation="MHVVLIKLKRMWLRAALFILFTITLAGTFSYAQNETDSDKFSYD
                     SNQVLHPEWYIKIIDWSFYTAARVAILSSVTIENTADVTYKDVRVNLIYTSYSAPAAG
                     IIATSTGMLPVTLPPKSKKTYLKSGLTMGAGNQNYDLSDIQVLSATPVKN"
     gene            708..1337
                     /locus_tag="VFJ67_00955"
     CDS             708..1337
                     /locus_tag="VFJ67_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7288976.1"
                     /translation="MKQKLLLLLALTAAVAGWALLAYLFFNYDFSEKYFGKVYRRQAP
                     EFNLTDQDGHPVSLKMFRDKVVLLEWGYTNCPDVCPLTLSKLNKVMEALGKRSADVQV
                     LFITVDPERDTAERLKSYVPYFNKSFIGVTGTPESIKKVAREYGVTVVKHEEVYGRSE
                     KDHWDRYLMTHTNTVFLVDSDGILLLTYPHYLLEPDKIAADIKRLLREE"
     gene            1384..2853
                     /locus_tag="VFJ67_00960"
     CDS             1384..2853
                     /locus_tag="VFJ67_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HET7288977.1"
                     /translation="MKFHAKAACTISLILIVSFFSVSGCDNNKHKPLGEPFRNPPIIS
                     SVDGVIDEEFDVLYSDNEVNGKQFTSPTYNGLYTPPVLKVNPGDTIRLRVRNFIDQMT
                     NVHYHGMNVSPLSPSDDIFIMIPPTGDFDYVVQIPEDHPEGIFYYHAHLFGLTEYQIM
                     SGLSGGLIVNGILDPFPQLDGITQQVMYLKDVQIIDGTVPQGDDIDISAPTNPTVNGQ
                     TNPVLSIRPGETQFWQIANIGADHYYDLVLEGHEFYEIERDGNRHNQTVVKDEEYMPT
                     ASRVGVLVQGGKPGVYRLRAKKISTGPQGDQYGGETVLTMVVEGDPVEDPIELPISQF
                     EFPVVENLCDQPVQRSRNFIFSETENGDTFFINGKEFDPERVDTVVQIGNLERWTIQN
                     TSQELHVFHIHLTDFQVCEINGVEQPFIGYQDTVNLPYDGQDPAWEGPGEVVIVIDFR
                     NPIIEGKAVYHCHIGQHEDNGMMAVFEACFADECPPEEM"
     gene            complement(2914..3933)
                     /gene="obgE"
                     /locus_tag="VFJ67_00965"
     CDS             complement(2914..3933)
                     /gene="obgE"
                     /locus_tag="VFJ67_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET7288978.1"
                     /translation="MVKFIDEAKIYVKSGAGGRGCVSFRREKYVPHGGPNGGDGGNGG
                     DVILVTNENMSSLLDHRYKQHYKAKRGEHGKGKDMHGRNADTLYIPVPVGTIVRDFET
                     GEVLGDLTQKDETLLVAKGGRGGKGNARFTTSTNQAPKIAEPGGEGEEKTLLLELKLL
                     ADVGIIGFPNAGKSTLISRISAARPKIADYPFTTLVPNLGVVSYGDGKTFVIADIPGI
                     IEGAHEGAGLGIQFLRHVERTKLLIHLLDLSPATGRDPIDDYETLNRELAAYSPELSK
                     KPQIVAPNKIDITEAREKLKEVKKYFDKKGITIYPVSSATGEGLKELLLETGKRLEAL
                     ADDNQ"
BASE COUNT          910 a         1022 c         1057 g          972 t
ORIGIN      
        1 ccccggatgc acggatagta ccggccgtta gaccgagcat caatataaaa agcgaaagta
       61 tctttagtat ggtccgggga gaaaatattc cgtgcccaca tctgttggaa atttctctca
      121 tagcggatta tcgaatccta aagtgccgtc gtcggacgca ttgttcagtt tttaaccggg
      181 gtcgccgaca agacctgtat atcgctcagg tcgtaattct gattcccagc gcccatcgtc
      241 aggccgctct tcagatatgt ctttttgctc ttgggcggca gtgttaccgg aagcatgccg
      301 gtcgacgtgg ctattatgcc cgcagccggc gccgagtagg aggtatatat gagattgacc
      361 cgtacatcct tgtacgtcac atcggctgtg ttttctatcg taaccgagct caggatcgcc
      421 accctcgcgg ctgtgtagaa ggaccagtct atgatcttta tataccactc gggatggagg
      481 acctggttgc tgtcgtagct gaacttgtcg gaatcggtct cgttctgggc gtaagaaaag
      541 gtgcccgcaa gagtaatcgt aaataaaatg aataaagctg cccttagcca cattctttta
      601 agtttaatca aaactacgtg catattccac aattatctct cggattatac tcgggttctc
      661 ctttatattt aggagcactc cgggttataa ttactggctc tgaagccatg aaacaaaaac
      721 tcctcttgct gttagccctg acggccgcgg tcgccggatg ggctctcctc gcatatctct
      781 tcttcaacta tgacttttcg gagaagtatt tcggcaaggt ttacaggcgg caggctcccg
      841 agttcaacct gacggatcag gacggacacc ccgtatcact caagatgttc cgtgataaag
      901 tagtccttct ggaatggggg tacacgaact gcccggacgt gtgtcccctg actctctcga
      961 agctcaataa ggtgatggaa gccctcggga aaagatcggc ggacgttcag gtgttgttca
     1021 taacggttga ccccgagagg gacacggccg agaggctcaa gtcatacgtt ccttatttca
     1081 acaagagttt tatcggtgtg accggcactc ccgaaagtat caagaaggtc gcgagggagt
     1141 acggcgtcac ggtcgtcaaa cacgaggagg tatacgggag gagtgaaaag gaccattggg
     1201 acaggtatct gatgactcat accaataccg tttttctagt cgacagcgac gggatccttc
     1261 tccttacata ccctcactac cttctcgaac ccgataaaat cgcagcggat attaaaaggc
     1321 tattgcggga agagtaaaat gtccctaaaa tgtgtatgtt caggaagttg gataggaggg
     1381 tgtatgaaat ttcatgcaaa agccgcgtgt acgatttcgc ttattctgat cgtttccttt
     1441 ttttcggtaa gcgggtgcga taataacaaa cacaagcccc tcggagagcc gttccgtaat
     1501 ccgccgatca tatcgagcgt ggacggggta atcgacgagg aattcgacgt actttattcg
     1561 gataacgagg tgaacggcaa gcagttcacg agcccgacgt ataacggtct ctacaccccg
     1621 cctgtcctga aggtaaatcc gggggatacg atcaggctca gggtcagaaa cttcatcgac
     1681 cagatgacga acgtgcatta ccacggcatg aacgtaagtc ccctgagccc ctccgacgat
     1741 attttcatca tgatcccgcc gacgggagac ttcgactatg tggtgcagat accggaagac
     1801 catcccgaag gtatattcta ttatcatgcc cacctattcg ggctcacgga atatcagata
     1861 atgagcggac tttctggcgg attgatcgtc aacggaatac tcgacccttt cccccagctc
     1921 gacggtataa ctcagcaggt gatgtactta aaggacgtgc agataatcga cggtacggtc
     1981 ccgcagggtg acgacataga cataagcgcg cctacgaacc ctactgtgaa cggacagacg
     2041 aaccccgttc tatctatccg gcccggagag acacagttct ggcagatcgc gaacataggc
     2101 gcggaccatt actatgatct ggtgctcgaa ggccacgaat tctacgaaat agagagggac
     2161 gggaacaggc ataatcagac cgttgtaaag gacgaggaat acatgccgac cgcttcaagg
     2221 gtcggagtgc tcgtacaggg aggcaagccc ggggtttaca ggctaagggc gaagaagata
     2281 agcaccggcc cccaggggga ccagtacgga ggagagacgg tgctgacaat ggtcgtcgag
     2341 ggcgaccccg tcgaggaccc gatagagctt ccaatttctc agttcgaatt cccggtcgtg
     2401 gagaacctct gcgaccagcc cgtacagagg agcaggaact tcatattctc tgaaaccgag
     2461 aatggcgaca cgttcttcat caacggaaag gaattcgatc ccgagcgtgt cgatacggta
     2521 gtccagatag ggaatcttga aaggtggacc atacagaata cgtctcagga gctccatgtg
     2581 ttccacatac accttacgga ctttcaggtg tgtgagatta acggcgtcga gcagcctttc
     2641 atcggatatc aggatacggt aaacctcccg tatgacggac aggaccctgc ctgggagggg
     2701 ccgggcgagg tcgtgatagt gatagatttc aggaacccga tcatcgaggg caaggcagtc
     2761 tatcactgcc acatcgggca gcacgaggat aacggtatga tggccgtgtt cgaggcgtgc
     2821 ttcgccgacg aatgccctcc ggaagagatg taggaggcgc tgatttcgta ttgatattat
     2881 aagttaagta tataggttat agtgctgtct atattattga ttatcgtccg ctaatgcctc
     2941 aagcctcttt ccggtctcaa gtaggagttc cttgagcccc tctcccgttg cggaagagac
     3001 gggataaatc gtgattccct ttttgtcgaa atattttttt acctctttga gcttctctct
     3061 cgcctccgtg atgtctattt tgttaggcgc gactatctgg ggcttcttcg atagctccgg
     3121 gctgtaagcc gcaagctccc tgttaagtgt ttcgtaatcg tcgatcgggt ccctgcccgt
     3181 ggccggtgag agatcgagaa ggtgtatgag gagcttagtg cgctcgacgt gcctcaggaa
     3241 ctggatgccg agaccggctc cctcatgagc gccttcaatt atgcccggta tgtccgcgat
     3301 cacgaacgtt tttccgtcgc cgtagcttac gacgcccagg ttgggcacga gtgtcgtgaa
     3361 cggatagtcg gctatcttcg gccgggcggc ggatattctg gatatgagcg tcgatttccc
     3421 cgcgttcggg aacccaatta tcccgacgtc ggcgaggagc ttcagctcca gtagcaatgt
     3481 cttctcttcc ccctctccgc cgggctctgc gatcttcggg gcctggttgg tcgatgtcgt
     3541 gaaacgggcg ttcccctttc cgcccctgcc gcctttcgcg acgagaagcg tttcatcttt
     3601 ttgtgtgagg tccccgagta cctcacccgt ctcgaagtct ctcactatcg ttcctacggg
     3661 tacggggatg tagagcgtgt cggcgttcct gccgtgcatg tcctttccct ttccgtgctc
     3721 gccccgcttc gccttatagt gctgtttgta cctgtggtcg aggagcgaag acatattctc
     3781 gttcgttacg agtatgacgt ctcctccgtt tccgccgtcg ccgccgttgg gccctccgtg
     3841 ggggacgtac ttttccctcc tgaaactgac gcagcccctg ccgcccgctc ccgacttcac
     3901 gtatatcttc gcctcatcga tgaatttgac catgggctga tattataatc gataatgaaa
     3961 a
//
LOCUS       DASSJH010000044         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1523271, whole genome shotgun sequence.
ACCESSION   DASSJH010000044 DASSJH010000000
VERSION     DASSJH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523271"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFJ67_00970"
     CDS             complement(<1..352)
                     /locus_tag="VFJ67_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288979.1"
                     /translation="MRLYKTYLIAAVIVSLAVAACNKKPEYVPGSSVQGFRAAISKIL
                     FSPIAFDGATVAVEGIVAQVKEVNPEQEDASTEFKLVDLNGNFINVDMPGSWEIADDD
                     YIIVGGIYRKNGNIL"
     gene            515..1321
                     /locus_tag="VFJ67_00975"
     CDS             515..1321
                     /locus_tag="VFJ67_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HET7288980.1"
                     /translation="MDTFSGDGYGIARIRAPESDSNYNYVVWCRETSACAVIDPFDPV
                     TVLNFIRDNGLSVKYIINTHCHPDHIKGNDPILKVTLSEILVHPLGSDLVSPRSRAVN
                     DGDRINIGKIGMDITHTPGHCPEHIILVMGENVFTGDTLYLAGTGNLKHRGDVDELFK
                     TMETKIRTLPDGLRVFPGHDYADANLRFALDLEPGNKAAKKKLADISKLAKKGKEPGP
                     TTIGEEKTYNPFLRYDSPAITKELRKRNPSLGDSSASYFTELRKLRDEWK"
     gene            1379..>2654
                     /locus_tag="VFJ67_00980"
     CDS             1379..>2654
                     /locus_tag="VFJ67_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7288981.1"
                     /translation="MKTKDSESLKAIRGLLEKRILFIDGAMGTMIQRHRPGEQDFRGE
                     IFKDHPKDLKGNNDLLCMTRPDIVAGIHRSYLEAGADIIETNTFNANSISQSDYGLEH
                     IVYELNKSAAALARKTADEFMSEHPDRKCFVAGALGPTTKSASISPDVSNPAARGVTF
                     DELVEAYYEQLMGLVDGGIDIILAETTFDTLNLKAAIYGIYRFLDEHKERLPLMISVT
                     ITDASGRTLSGQTIEAFWNSVRHARPLSVGINCALGAAEMRPYIEELSGIADCYISCY
                     PNAGLPNPLSDTGYDQTPADTSSYLEDFASSGFINIVGGCCGTTPEHIRAIAEKVSKY
                     PPRRVPVIQSATRLSGLEALNIGKDAPFILVGERTNVTGSPRFAKLVKEDDFEGALAV
                     AKQQVENGANVIDVNFDEALLDGEACMTRFLNL"
BASE COUNT          682 a          743 c          731 g          498 t
ORIGIN      
        1 cgagtatatt gccgtttttc ctgtagatac cgcccactat gatataatcg tcgtccgcta
       61 tctcccagga gccgggcata tccacgttga tgaaattccc gttcagatcg acgagcttga
      121 actcggtgct ggcgtcttcc tgctcgggat tgacctcctt tacctgggcc actataccct
      181 ctacggccac cgtagctccg tcaaaggcga tcggagagaa gaggattttc gatatcgccg
      241 ccctgaaccc ctgtacgctc gaacccggaa catattcggg ttttttatta catgcggcta
      301 cggcaagaga aacgatcacg gccgcgatca gataggtctt gtagagtctc aacttctgcc
      361 cccttttttc ccgcccgcat gggatttatg ataaataacg gttcaggctt tatattttac
      421 cctacacggt aaattaatat taattttctg tgtcataata agtgacgcct ccggcagggg
      481 ccggggagaa cggataaaag agggacaagc gcgaatggat actttctcag gtgacggata
      541 cggcatagcg aggataagag ccccggagag cgacagtaat tacaactacg tagtctggtg
      601 cagggaaacg tccgcgtgcg cggtcatcga cccgttcgac cccgtgaccg tactgaactt
      661 catcagggac aacgggctga gcgtaaaata cataatcaac acccactgcc accctgacca
      721 cataaaaggg aacgacccga tccttaaagt gacgctctcc gagatactcg tccatccgct
      781 tgggagcgac ctggtctccc cgaggtcgag agccgtgaac gacggggaca ggataaatat
      841 cgggaagatc ggcatggaca ttacccatac gcccggacac tgccccgagc atataatact
      901 ggtcatggga gagaacgtat tcacggggga tacgctctat cttgcaggca ccgggaacct
      961 gaaacacaga ggggacgtag acgagttgtt caagactatg gagacgaaga taaggacgct
     1021 cccagacggg ctccgcgtat tccccggcca cgactacgcc gacgccaatc tgaggttcgc
     1081 gctcgacctc gagccgggca ataaggccgc gaaaaagaag ctcgccgata taagcaagct
     1141 cgcgaaaaaa ggaaaggagc ccggcccgac gacgataggg gaggaaaaga cgtataaccc
     1201 gtttctcagg tacgactctc ccgcgataac aaaggagctc agaaagagga acccgtccct
     1261 cggagactca agcgcatcat atttcacgga gctcagaaag ctcagggacg aatggaagta
     1321 aggcagtccg ccgccggccg ctccgccccc aaagactcat ttggtataat aaaagcttat
     1381 gaaaacaaag gattcggaat cgctgaaggc cattcgcggc ctcctcgaaa aacgcatact
     1441 cttcatagac ggcgctatgg gcacgatgat acagcgccac agacccggcg agcaagactt
     1501 caggggcgag atattcaaag accaccccaa ggatctcaag ggtaataacg acctcctttg
     1561 catgacgagg cccgacatcg tggccggcat acacaggagc tacctagagg cgggggcgga
     1621 cataatcgag accaatacgt tcaacgccaa cagcatatcg cagtcggact acgggctcga
     1681 gcatatagtt tacgaattga ataaatcggc agcggcgctg gcgagaaaaa ccgcggacga
     1741 attcatgtct gagcatccgg acaggaaatg cttcgtcgcg ggcgccctcg gcccgacaac
     1801 aaaatcggcg tccatttcgc ccgacgtctc taatcccgcc gcgagagggg tcactttcga
     1861 cgagctcgtg gaggcctatt acgagcagct catgggactc gtcgacgggg gcatcgacat
     1921 catactcgca gagacgacgt tcgacacgct caacctcaag gccgccatat acggaatata
     1981 caggttcctc gacgagcata aggagcggct cccgctcatg atttcagtca ctataacgga
     2041 cgcctccggc aggaccctgt cagggcagac gatagaagcc ttctggaact cggtgagaca
     2101 tgccaggccg ctcagcgtcg gcataaactg cgccctcggg gccgccgaga tgcgccctta
     2161 catagaggag ctatcgggaa tagccgactg ctacataagc tgttatccga acgcggggct
     2221 gccgaacccg ttgagcgaca cggggtacga ccagaccccg gcagacactt cgtcctatct
     2281 agaggatttc gcctcgagcg gtttcatcaa tatcgtgggc gggtgctgcg gcactacgcc
     2341 cgagcacata agggcgattg cggagaaggt gagcaaatac ccgccccgga gggtccccgt
     2401 aatccagtcc gctacccgtc tgagcggtct cgaagcgtta aatataggga aggacgcgcc
     2461 ttttatattg gtcggagaaa ggacgaacgt cacgggctcc ccgaggttcg cgaagctcgt
     2521 caaggaggac gatttcgaag gggcgctcgc tgtcgcaaaa cagcaggtcg agaacggcgc
     2581 taacgtaatc gacgtcaact tcgacgaggc tctcctcgac ggcgaggcct gtatgacgag
     2641 gtttctcaac ctcg
//
LOCUS       DASSJH010000045         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1090737, whole genome shotgun sequence.
ACCESSION   DASSJH010000045 DASSJH010000000
VERSION     DASSJH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090737"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /gene="glmU"
                     /locus_tag="VFJ67_00985"
     CDS             <1..976
                     /gene="glmU"
                     /locus_tag="VFJ67_00985"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7288982.1"
                     /translation="GYGRVLRNGKGEVERIIEDKDASRAEKKITEINSGTYCVKSGFL
                     WDALRSIGSGNKQKEHYLTDIIGIAEKRSLKILGIPAADPREALGINDRVQLSEVEAI
                     LKHRVNESLMRGGVSMIDPETTFISPGVRIGEDTVIYPDTYIYGKTVIGGGSSIGPGV
                     WIEDSVLGSGVTVKLSSFITNSVIGDNVTVGPFAHLRPEAEIMEGAKIGNFVEIKKSR
                     IGRGSKVPHLSYVGDAVIGKDVNIGAGTITCNYDGYRKYETQIADDVFIGSDTMLVAP
                     VSVGRGATTGAGSTITKDVPEGALAIGRARQTVIENWSRKPREGKGKK"
     gene            976..2808
                     /gene="glmS"
                     /locus_tag="VFJ67_00990"
     CDS             976..2808
                     /gene="glmS"
                     /locus_tag="VFJ67_00990"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET7288983.1"
                     /translation="MCGIVGYVGDRKKTVGVLLDGLTRLEYRGYDSSGIALMDDGHPR
                     VFKSTGKLENLKKKIAKHPLEGLLGIGHTRWATHGDATEKNAHPHVSAGTSVVHNGII
                     ENYSELKEELIEKGFKFYSDTDTEVLAHLIESCCGGDLSFEDGVRAAFRRVEGSYAVA
                     VISDRAPDKVIATRRFSPLIVATGDRENYLASDVPAILPYCRNVIFLEDGDVAVLDKN
                     SVTITDIDGEPVKRDVQTINWDPVSVEKCGYRHFMIKEIHEQPQAVLDTMRGRFSEET
                     GDVFFEGFEGSPYKDVNRIMALACGTSYHASLIGKYMIETLARVPVQVEIASEFRYRN
                     PILDKHTLAIAVSQSGETADTSEALFEARAGGAKTLGITNVLMSKIARDAERVIYTHA
                     GPEIGVASTKAFTTQLLVFYLLSVYLGRIKKQIDRERAAELIRDAIKLPQLMQMTLRL
                     DDEIKELAKEFFHYRNFLYLGRGISYPVAFEGALKLKEVSYIHAEGYAAGEMKHGPIA
                     LIDDEMPVVLIAPNDGITYRKILGNLQEVRARRGKIIFLTSDTTPEELRGSVDRVIEI
                     PGCNYLLSPLISVIPLQLLAYHIAVLKGTDVDQPRNLAKVVTVE"
     gene            2970..>3035
                     /locus_tag="VFJ67_00995"
     CDS             2970..>3035
                     /locus_tag="VFJ67_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288984.1"
                     /translation="MKVLVVGSGGREHALCWKIKQS"
BASE COUNT          783 a          790 c          893 g          569 t
ORIGIN      
        1 gggttacggc agggtgctga ggaacggcaa gggagaggtc gagaggataa tagaggacaa
       61 ggacgcttcc cgggctgaga agaagatcac ggagataaac tccgggacct attgtgtcaa
      121 atccggcttc ctttgggatg ccctcagaag tatagggtcc ggcaataaac aaaaggagca
      181 ctatctgacc gacattatcg gaatagccga aaagagatcg cttaaaattt tagggatacc
      241 ggcggccgac ccgcgcgaag ccctcgggat taacgacagg gtccagctct ccgaggtcga
      301 ggcaattctg aagcacaggg tcaacgaatc ccttatgcgg ggaggcgtct ccatgatcga
      361 cccggagacg accttcattt ctcccggcgt caggatcggg gaagacacag tgatctatcc
      421 tgatacctat atttacggta aaacggttat cggcggaggc tcgtccatag gcccgggcgt
      481 ctggatcgag gactccgtgc tcggaagcgg cgttacggtc aagctctcct ccttcattac
      541 gaattccgta ataggggaca acgtcacagt cgggcccttc gctcacctga ggcccgaggc
      601 ggagataatg gagggcgcca agatagggaa cttcgtcgag atcaaaaaat cccgcatagg
      661 gcgcggctcg aaggtccccc acctctcata cgtcggggac gcggtcatag ggaaggacgt
      721 taatataggg gcggggacga tcacctgcaa ttacgacggg tacaggaagt acgagactca
      781 aatagccgac gacgtgttca tcggcagcga taccatgctc gtggccccgg taagcgtagg
      841 caggggcgcc accacgggcg cgggatcgac cataacgaaa gacgtccccg aaggggcgct
      901 cgcgatcgga agggccaggc agacggtgat cgagaactgg agcagaaagc ccagagaagg
      961 gaaggggaag aaataatgtg cggaatagta ggttacgtcg gcgaccggaa aaaaacggtg
     1021 ggcgtgctcc ttgacgggct cacgaggctc gaatacaggg gttacgattc gtccggtatc
     1081 gcgctcatgg acgacgggca tccgagggta ttcaagagca cgggcaagct cgagaacctc
     1141 aagaaaaaga tcgcgaagca ccccctcgag ggattgctag gcataggaca tacgaggtgg
     1201 gccactcacg gcgacgcgac cgagaagaac gcacaccccc acgtatctgc ggggacgtcc
     1261 gtagtccaca acggcataat agagaattac tccgagctca aggaagagct aatagaaaag
     1321 ggcttcaagt tttactccga caccgatacg gaggttcttg cgcacctaat cgagagctgc
     1381 tgcggagggg acctctcatt cgaagacgga gtgagagcgg ctttcaggag ggtcgaaggg
     1441 tcatacgccg tagcggtgat atccgacagg gcccccgaca aggtgatagc caccaggagg
     1501 ttctcgccgc tcatcgtagc cacaggagat cgcgagaact atctcgcgtc ggatgtgccg
     1561 gcgatactgc cttactgcag gaacgttata ttcctcgagg acggcgacgt cgctgtcctc
     1621 gataagaaca gcgtaactat caccgacatc gacggcgagc cggtgaagag agacgtgcag
     1681 acaataaact gggaccccgt ctcggtagag aagtgcggat accgccactt catgataaag
     1741 gagatacacg agcagcccca ggctgtgctc gacaccatga ggggcaggtt ctcggaagag
     1801 accggtgatg tgtttttcga aggattcgaa gggtctccct ataaggacgt caaccgtata
     1861 atggccctag cctgcggcac ttcttatcac gcttccctca tcggtaagta catgatcgaa
     1921 actctagcca gagtccccgt tcaggtggag atagcctctg agttccgcta ccgtaatccg
     1981 atcctcgaca aacacacgct cgccatagct gtatcccagt cgggggaaac ggcggatacg
     2041 tctgaagcgc tgttcgaagc cagggcaggt ggggctaaga cgctcggtat tacgaacgtg
     2101 ctcatgagca agatagccag ggacgcagag agggtgatat acactcacgc gggcccggag
     2161 atcggcgtcg cgtccacgaa agctttcacg acacagctcc tggtctttta tctcttgtcc
     2221 gtttacctcg gcaggattaa gaaacagata gacagggaaa gggccgccga gctcatcagg
     2281 gatgccataa agctccccca gctcatgcag atgacgctca ggctcgatga cgagataaaa
     2341 gagctggcta aggaattctt ccattacagg aacttcctct acctggggcg cgggatcagc
     2401 tacccggttg ctttcgaagg ggcgctcaag ttaaaagagg tctcctacat acacgcggaa
     2461 ggatatgccg caggcgagat gaaacacggt ccgatcgcgc tcatagacga cgagatgccc
     2521 gtcgttctca tagctcccaa cgacggcatt acgtacagga agattctcgg gaacctccag
     2581 gaagtaaggg cccggagggg aaagatcatc tttctcacat ccgacaccac tcctgaagag
     2641 ctcaggggca gcgtcgaccg cgtaatagag atacccgggt gcaactatct gctgagcccg
     2701 cttatatcgg tcatccctct tcagctgctc gcttaccaca tagcagtgct caagggcacg
     2761 gacgtcgacc agcccaggaa cctggccaag gtcgtcactg tcgagtagcg ccgttttagg
     2821 atagactata ttcctgttaa tctgaatctg cggaaaccca tactccacct ccggtaattg
     2881 ttcgcggctc atttccaaat gcgataatat ctggtagatt tacgggcttc tcgcagtatt
     2941 aaccgaaaaa taaagaggtc gagaattcaa tgaaagtgct tgttgtcgga tcaggcggca
     3001 gggaacacgc tttgtgctgg aaaataaagc agagt
//
LOCUS       DASSJH010000046         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_226198, whole genome shotgun sequence.
ACCESSION   DASSJH010000046 DASSJH010000000
VERSION     DASSJH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226198"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFJ67_01000"
     CDS             <1..1017
                     /locus_tag="VFJ67_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7288985.1"
                     /translation="KYFFEGVEKINVEDLMKIPEDWLQKSFVNALTDEEKKLIKSMGG
                     LDKLLERLKELMEEQKKRHQGGNKWIGTGGTSPFGAYGYNPEGIRIGQEESRHRRAVK
                     VWDKREFRDLDDGVELQTRNMKMALRRLRILTREGVREELDLDKTIDRTSRNAGLLEL
                     EMVPAKKNNVKVLLFLDIGGSMDDHIELCSRLFSAAKYEFKHLEHYYFHNCLYEFVWR
                     NNKRRWQETVPTFQVLHTYNSDYKVIVVGDASMSPYELLYQNGSVEHNNDEPGFTWLE
                     RLKTHYPDIVWLNPVPVKQWKYTESIGMVRKFMNDRMFPLTMGGLQQAIRALKDKKVK
                     FEGH"
     gene            1056..1514
                     /locus_tag="VFJ67_01005"
     CDS             1056..1514
                     /locus_tag="VFJ67_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7288986.1"
                     /translation="MPLAIDHINIVVSDLEKSVRFYTELLGFNESKRAHLEGEWIESI
                     VGLKGVIADVAFIVAPAGEPRIELLCYKIPRGEQVPANSLANTPGLRHIAFRVDDINA
                     AIEKLKSGGVKLLGEPVVVPETVVTHDAGHKMLCYFHDPDGTLLEITEYK"
     gene            complement(1567..1986)
                     /locus_tag="VFJ67_01010"
     CDS             complement(1567..1986)
                     /locus_tag="VFJ67_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7288987.1"
                     /translation="MILFLTVPSHSETVQEDAAPGDYNQAVGGSGFDKVEVISQGRLV
                     DLIEYLVPGKYTAFYFYAEWCGPCRILKPKLEKFVENGDKLVLRELDIINWENPLVTY
                     YKLTSIPYFIIYGPDGKFIERGPGFSNDLMKEISSPD"
     gene            complement(2084..2965)
                     /gene="purU"
                     /locus_tag="VFJ67_01015"
     CDS             complement(2084..2965)
                     /gene="purU"
                     /locus_tag="VFJ67_01015"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HET7288988.1"
                     /translation="MSKKEETGDSRSAILLVHCPDRKGLVAAITEFIFKNEGNIIYLD
                     QHVDTQDKVFFMRVEWDLGNFSIPAGKIGEYFQTLIAEKFGMKWSLYFSDYRPRMAIF
                     VSKLPHCLHDILSRWQSEQWNVDIPLIISNHDDLEQVASTHGIDYHVTPDVKENKRES
                     EEKQLGLLKEYGIDFVVLARYMQILSEEFVSHYPNRIINIHHSFLPAFPGARPYHSAH
                     ERGVKIIGATSHYVTADLDSGPIIEQNVVRITHKDSVEDLIRKGRDLEKVVLSQAIWY
                     HIKRKILVYGNRTLIFD"
     gene            complement(3005..>3394)
                     /locus_tag="VFJ67_01020"
     CDS             complement(3005..>3394)
                     /locus_tag="VFJ67_01020"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET7288989.1"
                     /translation="NCRLSALNRNQFEQLVDKAYWNIPEQFRNKIDNVVIKVEDYPSA
                     EDKEKLEIRGEGLLLGLYRGTPLHLRSVWQGLRMPDEIVLYQKDIENVCRNDREIEEK
                     INEVLKHELGHYFGLSDDEIYELMGRD"
BASE COUNT          798 a          871 c          917 g          808 t
ORIGIN      
        1 aagtatttct tcgaaggcgt tgagaagata aacgtcgagg acctgatgaa gataccggaa
       61 gactggcttc agaagagctt cgtcaacgcc ctcacggatg aggagaagaa gcttatcaaa
      121 tcaatgggcg gcctcgacaa gctgctcgag cgactgaagg agcttatgga ggagcagaag
      181 aagagacacc agggcgggaa taaatggatc gggaccgggg gcacgtcgcc gttcggcgcg
      241 tacgggtaca accccgaggg cataagaata ggccaggaag agagcaggca caggcgcgcg
      301 gtaaaggtat gggacaagcg cgagttcagg gacctcgacg acggagtcga gcttcagacg
      361 aggaacatga agatggcgtt gagaaggctc aggatactca cgcgcgaggg cgtgagggag
      421 gagctcgacc tcgacaagac gatagacagg acgtcgagga acgcggggct gctggagctg
      481 gaaatggtcc ccgcgaaaaa gaacaacgtg aaggtgctcc tcttcctcga catcggcggg
      541 tcgatggacg accacataga gctatgctcg aggctgttct cggcggcgaa atacgagttc
      601 aagcacctcg agcattacta tttccataac tgcctttacg agttcgtctg gaggaataac
      661 aaaaggaggt ggcaggagac tgtgccgacg ttccaggtcc tccatacgta caacagcgac
      721 tacaaggtta tagtggttgg agacgcctcc atgtctccct acgagctgct ttatcagaac
      781 gggagcgtcg agcacaataa cgacgagccc ggattcactt ggctcgaacg gttgaagacg
      841 cactatcccg atatagtctg gctcaatccc gtgcctgtaa agcagtggaa atacacagag
      901 tccatcggga tggtgaggaa gttcatgaac gatcgcatgt tcccgcttac tatgggcgga
      961 ctccagcagg caatcagggc ccttaaggac aagaaggtca agtttgaagg gcattgagcc
     1021 gaaagattaa tcgattgcaa taaaaaatta atttcatgcc tctcgcgata gaccacatca
     1081 acatcgtcgt ttcagacctc gaaaaatctg tcaggtttta tacggaactc ctcggattca
     1141 acgaatcgaa gagggctcac ctcgaaggcg aatggatcga gtcgatcgtt ggattaaaag
     1201 gagtcatcgc cgacgtggcg ttcatcgtag cccccgcagg agagccgagg atcgaattac
     1261 tctgctacaa gataccgaga ggagaacagg tcccggcgaa ctcccttgca aacacaccgg
     1321 gcctgaggca catcgctttc agggtggacg acataaacgc ggccatcgag aagctcaaga
     1381 gcgggggagt gaaattactg ggggagccgg tcgtcgttcc ggaaaccgtg gtcacgcacg
     1441 acgccggtca caagatgctc tgctacttcc acgacccgga cggtacgctc cttgaaatta
     1501 cggaatacaa gtgattggtc cgggcgtaaa cggattgagg cccttgtgac gaaccctggt
     1561 acacggttaa tccgggctcg atatttcttt cattaaatca ttggagaatc ccggtcctcg
     1621 ctcaatgaat ttcccgtccg ggccgtagat tatgaaatac ggtatcgacg tcagtttgta
     1681 ataggttacg agcgggtttt cccagtttat aatatcgagc tctctcaaga cgagcttgtc
     1741 gccgttctcg acgaactttt cgagcttggg cttcaagatt ctgcacggcc cgcaccattc
     1801 ggcataaaag tagaaggcgg tgtactttcc cggcaccagg tattcgatta agtcaacgag
     1861 tctgccctgg cttatcacct ctactttgtc gaagccgctt ccacctacgg cctgattgta
     1921 atcacccggc gcggcgtcct cttgaacggt ctcactatgc gagggaacag tgaggaacaa
     1981 aatcatcact cctgcagcag ccattcggat tagttttgat gtcatgttcg atgtgccttt
     2041 gagctcgata ttaaatatac gcgcgaaaca aacttgcgga ctatcaatcg aatatcagtg
     2101 tacggttccc gtaaacgagg atcttccgct tgatgtggta ccagatggcc tgtgacagca
     2161 ccaccttttc taggtctctc cctttcctta tgaggtcctc gacgctgtcc ttgtgcgtta
     2221 tgcgtacgac gttctgctcg attatgggcc ccgagtcgag gtcggccgtg acgtagtgac
     2281 tcgtggctcc tatgatcttc acccccctct cgtgcgccga gtggtagggt ctcgccccgg
     2341 ggaaggccgg aaggaatgaa tggtgtatat tgatgatcct gttcgggtag tgggacacga
     2401 actcttcgct caatatctgc atgtacctgg cgagcactac gaagtctatg ccgtactcct
     2461 tcagcaatcc gagctgtttc tcctctgact ctcttttgtt ctcctttaca tcaggcgtga
     2521 cgtggtaatc gattccgtga gtcgatgcga cctgttcaag gtcatcgtgg ttgctgatta
     2581 tgagcggaat atcgacgttc cactgctcgg actgccagcg ggagaggatg tcgtggagac
     2641 agtgggggag cttcgaaacg aatatggcca tacgcggcct gtaatccgag aaatagaggc
     2701 tccatttcat tccgaacttc tccgctatga gtgtctggaa atactcgcct atcttcccgg
     2761 cgggtatcga gaaattcccg aggtcccact cgacgcgcat gaagaatacc ttgtcctgcg
     2821 tatctacgtg ctggtcgagg tatatgatgt tcccctcgtt cttgaatatg aattccgtta
     2881 tggctgcgac gaggcccttt ctgtccgggc agtgcacgag cagaatcgcg gagcggctgt
     2941 cgccggtctc ttctttttta ctcatattat tattcactcc gtcgaagcgc ggttcccgga
     3001 cggctcagtc ccttcccatg agctcgtaaa tctcgtcatc ggagagcccg aaataatgtc
     3061 cgagctcgtg ctttagcact tcgtttatct tctcctcaat ttcccggtcg ttcctgcata
     3121 cattttctat atctttttga tacagcacta tctcgtccgg catcctgagg ccctgccaca
     3181 cgctccggag atggagaggc gtccccctgt ataacccgag aaggagacct tcgcccctta
     3241 tttcgagctt ctccttgtcc tctgcggacg ggtaatcctc gaccttgatg acgacgttgt
     3301 cgatcttgtt cctgaactgt tcagggatat tccagtaagc cttgtccact agctgttcga
     3361 actgatttct gttcaatgcg cttaatctgc aatt
//
LOCUS       DASSJH010000047         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_549987, whole genome shotgun sequence.
ACCESSION   DASSJH010000047 DASSJH010000000
VERSION     DASSJH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549987"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..2022
                     /locus_tag="VFJ67_01025"
     CDS             211..2022
                     /locus_tag="VFJ67_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET7288990.1"
                     /translation="MMTLQRKYIGSGSILLLVIAAALVAAGRALGIESDVSAPKWGMI
                     LMGLFGGLAIFLFGMDQMTDGLKAVMGEGMSTLLAKLTKNRFTATITGAITTAVIQSS
                     SVTTVIVIGFISVGLMTLQQVIGVILGANIGSTITAQIIAFNVTQYALLPIAVGFGMQ
                     FVSKTEKIRLYGGIIMGIGLVFYGMGVMSDSTHPLRTYQPFIDVMKEMSNPLLGISAG
                     LIFTGLVQSSAATIGIVIVLASQGAITLDAGIAIVLGANVGTCVTAILAAIGKPAPAK
                     QAAAAHILFNVIGVIIWLPLIGYLASLSEAISPVSANLTGTARLAADTPRQIANAHTL
                     FNIANTVIFIGFIGTFARLIQRLIPEKPEVLPEYATPKYLNDAFLETPALALDNIRLE
                     AVRLGGYVEELIEMARPAVLSGGKEDLDKIVSRANELRMLHDAMTDYARKLYSTEMKS
                     EEKRRLEALITVIVNLEHVGETVAVNMVSIGRERLEKRFKVSEETLTRFQPYSAKVRE
                     AFHLAIKALDLKDAVIAKRIAEMKPEIESLADGIVEHLSLRLVSGDPDRAVLYRVESQ
                     FVEIIQRIYYFAGMIANEIIQESGKARGERNPSLSPA"
     gene            2174..>2491
                     /locus_tag="VFJ67_01030"
     CDS             2174..>2491
                     /locus_tag="VFJ67_01030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET7288991.1"
                     /translation="MQVSVIIPTYERCDSLVRALTALSRQTFTYDKYEVIVSVDGSTD
                     STMEMLKGFQAPYELRTIWKPNAGRSAARNSGIREAKGEVLIFLDDDMEALPGLIERH
                     YERH"
BASE COUNT          610 a          659 c          677 g          545 t
ORIGIN      
        1 ctcgaaaatc acgagaatca ctcccgacta atagaaaaat aactccttta ttccggcagc
       61 tgaaccattg tcacatccct cccgctgagt ctttaccctg gacccggagc ttaatttaac
      121 gacacaaata tttatatttt cttatcatat ttggtggtaa cgtaatcagg aatacttctt
      181 tcgccttatc caatctcagg agggaaagat atgatgactc ttcaaagaaa atatatcgga
      241 tccggttcca ttctccttct ggtcattgcg gccgcgcttg ttgccgcggg ccgggcgttg
      301 ggaatcgaat cggatgtctc cgcccccaaa tggggaatga ttctgatggg tctcttcggg
      361 ggtctcgcta tattcctgtt cggcatggat cagatgacgg acggccttaa ggcggtcatg
      421 ggcgagggca tgagcactct cctagcaaag ctcacgaaga acaggttcac ggcgactatc
      481 acgggtgcga taacgaccgc cgtcatacaa tcttcgtccg tgactactgt tatcgtaatc
      541 gggttcatat ccgtgggcct tatgacgctc cagcaggtca ttggagtcat cctcggagcg
      601 aacatcggtt ccaccatcac ggcccagatt atcgcgttca acgtgacgca gtacgcgctc
      661 ctccctatag ccgtgggttt cggaatgcag ttcgtgtcca agaccgaaaa aatcaggctt
      721 tacggcggaa tcatcatggg gataggtctc gtattctacg ggatgggcgt gatgagcgac
      781 tcgacccacc ctctccgcac ataccagccg ttcatcgacg ttatgaagga aatgagcaat
      841 ccgttgctgg gcataagcgc cggcctgata ttcacgggcc tggtacagag ctcggccgcg
      901 accataggca tcgtaatcgt actcgcttca cagggagcaa tcacgctcga cgcgggcata
      961 gcgatagtgc tgggcgcgaa cgtcggcact tgcgtgacgg ccatactggc ggcgataggg
     1021 aagccagcgc ccgcgaaaca ggcggccgcc gctcacatcc tgttcaacgt aatcggcgta
     1081 atcatatggc tgccgttaat cggatacctc gcttccctct cagaagcgat ctcccccgtg
     1141 agcgcgaacc ttaccggtac cgcgcggctc gcggccgata cgccgcgcca gatcgcgaac
     1201 gcgcacacat tatttaacat cgcaaacaca gtcatattta tagggtttat aggaacgttc
     1261 gcgcgcctaa tacagcgcct catacctgaa aagccggaag tcctgcccga gtacgccact
     1321 cccaaatacc tgaacgacgc ctttttagag acacctgcgc ttgccctcga taacatccgc
     1381 ctcgaagcag tgcggcttgg cgggtatgta gaggagctca ttgaaatggc aagaccagcc
     1441 gtgctgtcag gcggcaagga agacctggac aaaatcgtat cccgcgcaaa tgagctccgc
     1501 atgctgcacg atgcaatgac ggactatgcc agaaagctgt attcgacgga gatgaaatcc
     1561 gaagagaaaa gacggctgga ggccctgata acggtcattg tcaacctgga gcacgtcggg
     1621 gaaaccgtag cggtaaatat ggtctctatc ggccgtgagc gtctggaaaa acggttcaag
     1681 gtaagcgaag aaacactcac aaggttccag ccatattcgg ccaaggtgcg cgaggcattt
     1741 catctggcga ttaaagccct tgatctgaag gatgcagtga ttgcgaagag gatcgcggag
     1801 atgaagccgg agatcgaaag tctggcggac ggtatagtcg agcatctctc actccggctc
     1861 gtcagcggcg atccggacag ggcggttctt tacagagtcg agagccagtt cgtagagatt
     1921 attcagcgca tatattactt tgccggaatg atagccaatg agataataca ggaatccggg
     1981 aaggcgcgtg gagagaggaa cccgtccctg agcccggctt aaattatgaa cggcggcgca
     2041 tattatttgc aatccggtcg gcctatggcc ggatcgccgg tgtcccggta ttccgggact
     2101 gtactttagc ccggagcagt atatcctttg agtgggtcag aataccggag cgaactgttc
     2161 agggccttaa agtatgcaag tcagcgtgat aatacctacg tacgagcgct gtgattcgtt
     2221 agtgcgggct ttaacagccc tttcccgcca gactttcaca tatgataaat acgaagttat
     2281 cgtgtcggtt gacggatcaa ccgattccac gatggagatg cttaaaggct ttcaagcgcc
     2341 ttacgagctc cgtacgatat ggaagcccaa tgcgggcaga tcggccgccc gcaacagtgg
     2401 catacgagag gcaaaagggg aagtcctgat attcctcgac gacgatatgg aagcgctgcc
     2461 cggacttatc gagcggcact acgagcgtca t
//
LOCUS       DASSJH010000048         5357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_659798, whole genome shotgun sequence.
ACCESSION   DASSJH010000048 DASSJH010000000
VERSION     DASSJH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659798"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..737)
                     /gene="lolA"
                     /locus_tag="VFJ67_01035"
     CDS             complement(60..737)
                     /gene="lolA"
                     /locus_tag="VFJ67_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HET7288992.1"
                     /translation="MKNIILAILGLLLTASAAYSAGGESIEVVVDRIQKKYEEIGDFH
                     ADFTQEATVKALDTVQKSDGEVWFKKPGKMRWNYYEPYKDQIVSDGQTLWFYNEEEKQ
                     VIETPLNQVSDTETTSTLLSGLGKIKELYKTSFTDSKEFQADGSYLIDLRPKEEGEEY
                     NKVTLAIDKGTMMVNTLYLYDPFGNLTKVLLKNIEIDKGVSDSLFNFQVPSGVEVIKP
                     PTSAAGQ"
     gene            complement(734..3049)
                     /locus_tag="VFJ67_01040"
     CDS             complement(734..3049)
                     /locus_tag="VFJ67_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET7288993.1"
                     /translation="MAKRKSSSRQEPSDYIVREIIAAFLFTAGLFSALSLVFYSGKTG
                     VDVKGAMGSIGVYISGILGMAFGVCAFVVPLVLFYTSVVVFINRPGGSLYRKTASAFL
                     LLVSVMVFLGLLYPDTELLGFTPGGGWVGDTVASVMRDSVAGTIGSYIIVTIILLVSL
                     IIISGITLSDLLGALGSVSVFIAEKSYSLSKYLVMAIGEIFVYLKESLSRPRMQPVSK
                     QARTGGNGLSRLNGRKKAEEDVELFDDDISEEEEYEEEDDEEEDDEDHKPEIVVEQPK
                     LKGLEDFFLKKEVIHKDYNLPPLDLLDPKTESGVEIDRDAVYEKARLIEEKLRDFGVS
                     GKVTEIRPGPVITMFEYKPAPGIKINRIASLESDLAMGLSALSIRIIAPIPGKDVIGI
                     EVPNAKRETVVLRELLEDVEFSKRKSMLTLALGKDISGLPFYMDLRKAPHLMIAGTTG
                     SGKSVLLNAVITSMLYKASPYELKFIMIDPKMLELSVYEDIPHLLHPVVTEPKKAVAA
                     LRWAVEEMEKRYRILSEEGVRDIETHNKNVEKLKSEDKWDKLLPYIVIVLDELADLMI
                     IAPSELKESITRLSQKARAAGIHLIVATQRPSADIVAGLIKANFPARISFLVSSKIDS
                     RIILDTGGAERLLGKGDMLFLEPGTSKLLRVQGALISDEEREGITEYVKSQGQPVYNE
                     QITYIEETEGGEDLEDEKDELYYEALRTIAETRQASISMLQRKLKIGYNRAARIVEIM
                     EKEGVVGPQEVAGKPREVYIDLSQISERHTS"
     gene            complement(3233..4696)
                     /locus_tag="VFJ67_01045"
     CDS             complement(3233..4696)
                     /locus_tag="VFJ67_01045"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET7288994.1"
                     /translation="MELSEVLNSTLLISPEISLIAAGIILMVLDPITKGEVKKNLFWV
                     ALAGLALAFVLNLKRFGGDMSAFSGALSLDQFAAYFNVIFLLGAAVAIVFSKDYMTGV
                     IHYTSEFYALILFSTSGMMILSSATEFMSLFLGFEIMSISVYVMSAFNRKSARSTEAG
                     IKYLVLGGFSSAILLYGIALLYGASGSIYLSEIFRTFDVANPLFVAGSALVLIGFIFK
                     IGAFPLHQWVPDVYEGAPMTATAFMSVGVKAAAFAILMRVIFEGLYELQVSIMPVLWI
                     VAIFTMLVGNIAAIAQKSIKRMLAYSSIAHAGYALVGVVAAYGGKELGVGSVIYYLFA
                     YTFMNLGAFGVLAYLSREGHECETFEDVAGLWGRRPFLALALGVFMFSLAGIPPTIGF
                     FAKYRIFLSAVQAGFYWLAVIGILSSVVSAYYYLKVLVYAYMKEETAAFPSFKIYSSV
                     ALVVLSVGTLVLGVFPLDSWDLAIKAAGSVLMTFHGG"
     gene            complement(4794..>5357)
                     /locus_tag="VFJ67_01050"
     CDS             complement(4794..>5357)
                     /locus_tag="VFJ67_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET7288995.1"
                     /translation="ESAGEKLEKLNPDVSVVLHEGHVTKDNIREMIRDYDIVVDGSDN
                     FPTRYLANDACYFEKKTLVSGAIFQFEGQVAVFKPHAGGACYRCLYPELPPKGLVPSC
                     QEAGILGAAAGAIGTMQALETLKQILEIGEPLTDRLLVFNALNMSFNSVGIKKDPRCR
                     LCGESPTIHSVEDISQPDCDIEGSPVS"
BASE COUNT         1226 a         1498 c         1329 g         1304 t
ORIGIN      
        1 gctacaaggc atatattcag aatcagaccc ctcatcggta aatcctcctt cccggttccc
       61 tactgccccg cggccgatgt cggaggtttt atcacctcga cgccgctcgg cacctgaaaa
      121 ttaaataaag aatccgatac ccctttgtca atttctatat tcttcagcaa tacctttgtc
      181 agattcccga aagggtcata aagatagagt gtgttcacca tcatcgtgcc cttatcgatc
      241 gcgagcgtga ctttattgta ctcctctcct tcttccttcg gtctgaggtc gataaggtag
      301 ctcccgtcag cctggaattc ctttgaatcc gtgaagctcg ttttgtagag ctcctttatc
      361 ttcccgagtc ccgagaggag agtcgatgtg gtctcggtat cggagacctg attgagaggc
      421 gtctctataa cctgcttttc ctcctcgtta tagaaccata gcgtttgtcc gtcagacact
      481 atctgatcct tatacggctc gtagtaattc catctcatct ttcccggttt cttgaaccag
      541 acctcgccgt ccgatttctg cacagtgtcc aatgccttaa cggtcgcctc ctgagtgaaa
      601 tccgcatgga aatcgcctat ctcctcgtac ttcttctgta tcctgtcgac gacgacttct
      661 atgctctctc cccctgccga atacgctgca ctcgccgtta ataataaccc caaaatcgct
      721 aaaatgatgt ttttcaactc gtatgcctct cgcttatctg acttaggtca atatatactt
      781 ccctaggctt cccggcgacc tcctgcggcc ccacaacgcc ctctttctcc attatttcga
      841 ctatcctggc agccctatta taaccaattt tgagcttcct ctggagcatg gaaatagagg
      901 cctgacgggt ctcggcgatt gtcctgagcg cctcgtagta tagttcgtcc ttctcgtcct
      961 cgaggtcttc gccgccttcc gtttcctcga tataggtgat ctgctcgttg taaacgggtt
     1021 gtccctggga ttttacatat tccgtaatcc cctccctctc ctcatccgat atgagcgccc
     1081 cctgtaccct caacagcttg gaagtgcccg gctcgaggaa gagcatatcc cccttcccca
     1141 ggagcctttc ggccccgcct gtgtcgagta ttatacggga gtcgattttc gatgatacga
     1201 ggaacgatat cctggccggg aaattcgcct ttataagccc cgccactatg tctgccgacg
     1261 ggcgctgcgt cgcaactata aggtggatgc ccgcggcgcg cgccttctga gagagcctcg
     1321 taatcgactc ctttaattca ctcggcgcaa taatcatgag gtcggcgagc tcgtcgagca
     1381 ctatgactat atagggcagg agcttgtccc acttatcttc cgatttgagt ttttcgacgt
     1441 tcttgttgtg cgtctctata tccctgacgc cttcctcgga caggatcctg tacctcttct
     1501 ccatctcttc cacagcccat ctgagcgcgg ccacggcctt tttaggctcg gtgaccaccg
     1561 gatggagcag atgcggtatg tcttcataga cggacagctc gagcatcttt gggtcgatca
     1621 taatgaattt aagctcataa gggctcgcct tgtacagcat gctcgtaatg accgcgttca
     1681 ggagcacgct cttccccgag ccggtggtgc ccgctatcat caggtgggga gcttttctca
     1741 gatccatgta aaaaggcagt ccggatatat ctttgccgag ggcgagggtg agcatcgact
     1801 ttctcttgga gaactccacg tcctcgagaa gctctctaag cactactgtc tccctcttcg
     1861 cgttcggcac ctctatgccg atcacgtctt tcccgggtat cggggcgatt atccttatac
     1921 tgagagcgct cagccccatg gcgaggtcgc tttcgagaga ggctatcctg tttatcttta
     1981 taccgggtgc gggcttatat tcgaacatcg tgatgacagg gcccggccgt atttccgtaa
     2041 cctttcccga gacgccgaaa tcccggagtt tttcctctat cagcctcgct ttctcgtaaa
     2101 cggcgtccct gtctatctcc acgccggatt ccgtcttcgg gtcgagcagg tcgaggggag
     2161 ggagattata gtctttgtga attacctctt ttttcagaaa gaaatcctcg agacccttga
     2221 gcttgggctg ctcgaccact atctcgggtt tatggtcttc gtcatcttcc tcctcgtcat
     2281 cttcctcttc gtactcttct tcctcggaga tgtcgtcatc aaagagttca acgtcctctt
     2341 cggccttctt tctgccgtta agcctgctga gcccgttccc ccccgtccga gcctgcttcg
     2401 agacgggttg catcctcggt ctggaaaggg attctttcag atatacgaaa atttcgccga
     2461 ttgccatgac taaatacttc gataacgagt aagatttttc ggctataaac actgagacgc
     2521 tcccgagcgc ccctagtaag tccgacaggg taattcccga tattattatt aaactcacga
     2581 gaaggattat ggttacaata atataagagc cgatagtacc ggcgacgctg tccctcatca
     2641 cggaggcaac ggtgtcaccg acccaccctc cgcccggcgt aaacccgaga agctcggtgt
     2701 ccgggtagag gagccccagg aacaccatca cagatacaag aagcaggaaa gcggaggcgg
     2761 tctttctgta aaggcttccg cccggtctgt ttatgaaaac gacgacggac gtatagaaga
     2821 gcacgagcgg tacgacgaag gcgcaaacac cgaacgccat tcccagtatt cccgatatgt
     2881 aaacgcctat cgagcccatg gcgcctttga catcgacgcc ggtttttccg ctgtagaaga
     2941 cgaggctcag ggccgaaaag agcccggccg tgaacaaaaa cgcggctatt atctcccgga
     3001 ctatatagtc ggaaggttct tgtctgctgc tcgattttct tttcgccatc tattcttcaa
     3061 gctaattata caatagtcgg gataagtatg caaaattgtt gattttcagt agcaagtgac
     3121 ggcgtctgcg aaaataaacg gaccaacgcg cccgtgcgga cgaggaggaa agagacggat
     3181 tttcgaaaac agtataagat ttattaataa atacaggata ttacaagggt gctcaaccac
     3241 cgtgaaacgt cataagcacc gagcccgcgg cctttatcgc caggtcccac gagtcgaggg
     3301 ggaagacccc tagaacgagt gtgcccaccg atagcacgac gagcgctacg gaagagtata
     3361 tcttgaacga ggggaaagcg gccgtctctt ccttcatgta tgcatacacg agcactttca
     3421 gatagtaata tgccgagacc acgctgctaa gtatgccgat caccgcaagc cagtaaaacc
     3481 ccgcctgaac cgccgacagg aatatcctgt acttggcgaa gaagccaatt gtggggggta
     3541 tgccggccag ggaaaacata aacaccccca gggccagagc gaggaaaggt ctcctgcccc
     3601 agagtccggc tacgtcctcg aacgtctcgc actcgtgacc ctcgcgtgag aggtaggcaa
     3661 gcaccccgaa cgcccccagg ttcatgaacg tataggcgaa gaggtaatag atgacactcc
     3721 ccacgccgag ctcctttccg ccgtaagcgg caacgacgcc tacaagggcg taacccgcgt
     3781 gggcgatgct cgagtatgcg agcatgcgct ttatgctctt ctgggcaatg gccgcgatat
     3841 ttccgactaa cattgtaaat atcgccacga tccagagcac cggcattatg ctcacctgga
     3901 gctcataaag cccctcgaaa atcactctca tcagtatagc gaaggcagcg gccttgaccc
     3961 cgaccgacat aaaagcggta gccgtcatcg gcgccccttc gtagacgtcc ggaacccact
     4021 ggtgaagcgg gaacgcgccg atcttgaata tgaatcctat cagcacgagc gcgcttccgg
     4081 cgacgaagag cggattcgcg acgtcgaagg tcctgaatat ttcgctaagg tatatagagc
     4141 ccgaagcccc gtagaggagt gctatcccgt aaaggagtat cgccgaggag aacccgccca
     4201 ggaccagata ctttattccc gcctccgttg agcgggcgga cttcctgttg aaagcggaca
     4261 tcacgtaaac ggagatggac attatctcga agccgagaaa aagggacatg aattccgtgg
     4321 ccgaggagag aatcatcatc cctgaagtcg aaaaaaggat gagtgcgtaa aattcgctcg
     4381 tgtagtggat gactcctgtc atatagtcct tcgagaagac gatagcgacc gctgccccga
     4441 gaaggaagat gacgttgaaa tacgccgcga actggtcaag cgacagcgcg cccgagaagg
     4501 cggacatatc gccgccgaac cgcttcagat tgaggacaaa ggccagagcg agacccgcga
     4561 gagcgaccca gaagaggttc ttcttcacct cgcccttcgt aatagggtcg aggaccatca
     4621 gtataatacc ggctgctatt aaagagattt cagggctgat tagcagggtg ctgttaagta
     4681 cttcgctcaa ttccattaag gaattctccg gcttaagtgt gtgtaaaacg cgaggtggat
     4741 tttcgggagt ttagtatgtg ggagtgacag ggggttgtca agttccgtgg acgtcaggaa
     4801 acaggggagc cttcgatatc gcagtcgggc tgggaaatat cctcgacgct gtgaatagtc
     4861 gggctctcgc cgcagagacg gcaccggggg tctttcttaa tccccacgga attgaacgac
     4921 atgttgagag cgttaaatac cagaagtcgg tcggtcagcg gctcgcctat ttccaatatc
     4981 tgtttaagcg tttccaaggc ctgcatagta ccgatagctc cggcggccgc gcctagtatc
     5041 cccgcttcct gacagctcgg aaccaggccc tttggcggaa gttctggata gaggcacctg
     5101 taacatgcgc cgcccgcatg gggcttgaat accgcgacct gtccctcgaa ctggaatatg
     5161 gctcccgaaa cgagcgtctt cttctcgaaa taacacgcgt cgttcgcgag gtatctcgtc
     5221 ggaaaattat cagacccgtc gacgactata tcgtaatccc ttatcatttc gcgtatgttg
     5281 tccttcgtaa cgtgaccctc gtgaaggacg acacttacgt ccgggtttaa tttctcaagc
     5341 ttctcgccgg ctgattc
//
LOCUS       DASSJH010000049         9542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_11022, whole genome shotgun sequence.
ACCESSION   DASSJH010000049 DASSJH010000000
VERSION     DASSJH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11022"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..453)
                     /locus_tag="VFJ67_01055"
     CDS             complement(16..453)
                     /locus_tag="VFJ67_01055"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7288996.1"
                     /translation="MIYRTLIFVIGLFLLDIVTAPLDDYSQRREVQGQTGQDSEDTGE
                     DGSLPEDGIVNLQGSINITDLILAVSEINDETYVIDGSVHPNEISIVTPGGGLRKEDV
                     LSLFDTVLRLNGLSVVKSDGINKVVNTSDIKGSATPVETEKQN"
     gene            complement(540..1508)
                     /locus_tag="VFJ67_01060"
     CDS             complement(540..1508)
                     /locus_tag="VFJ67_01060"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET7288997.1"
                     /translation="MPSIKDTRHQMVQFVFPEHANIHGTLHGGRLMDWIMLAGSINSS
                     RISRGITVLGATDSIDFINPVKVGDIVMLDSSIEYIGSSSMEIGVTVHSENLETREKK
                     LTTSAHLAFIAVDGDGNPRRINEQIEPADDEKALYEEARKRRDQRIPKIGIRDEQAKN
                     IIDETEITRMRLETTKVVLPEDSFYGSFMSVGKLMLDIDETAAILAMRFVKDVLVTGS
                     LDELFFYSPIKVGDITIFKSGITYVGNSSLEVGIKVLSENILTGEQRHTCTAFLTYVH
                     VGDDGLPKQVPAFSPETPEEKRLWKKAQERKDKRTARLKRIKEIAT"
     gene            1968..3266
                     /locus_tag="VFJ67_01065"
     CDS             1968..3266
                     /locus_tag="VFJ67_01065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7288998.1"
                     /translation="MEQNIKILLIEDNPDYAQLIKRILSKKTSCSMEVLHCDTFSSGY
                     EKASSGGIDLILLDLDLPDSGNINTLLSISEVTDVPIIVLTGTDDESLALKAVQMGAQ
                     DYLMKGQVDARLLVRSIMYAIERKKAEDAVKQTEERFKLLIENALDLIEMLDVDGTMK
                     FVSPSHKRVLGYENDDLIGRRIFEFVHPEDLPGVIDVFNETLQNPDRPYSAEFRVRHK
                     EGYWITLESIGKHCPPELSSIGVIVNLRDITERKKMEERLRSLSITDELTGLYNRRGF
                     LTFADHHIKAAERRKESVLLILIDLDGLKQINDAYGHNIGDQALMDAAQVIKQTFRST
                     DVTARISGDEFVVVTTDSDGNGQESIKRRIEEKLKEYNSKGIKPYHLSFSYGVATFDP
                     QDPSSIDRLLVKADELMYLNKNNKYVDTNENVLNLNRSKK"
     gene            3569..5515
                     /locus_tag="VFJ67_01070"
     CDS             3569..5515
                     /locus_tag="VFJ67_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7288999.1"
                     /translation="MSARLIKTLLVEDNDSYSRLVVKMLGGADNSGFDIESVDTLAKG
                     ITVLEEKEFDLVLLDLQLPDSEGLRTFTGIHERHPGIPVIVLTGTSDEDTAVKAVQLG
                     AQDYLVKGEMEILQLSRSIKYALERQREINKRKLTEQALFEEKERLAVTLASIGDGVI
                     TTDTEGKVTLINRVAERLTGWSHAEAAGRFIEEVFFIVNVETGEKKENPVSRAINANN
                     VVGLESGTVLITKGREGCRYVSASSSPIRDRDGIIIGVVLVFRDITELKKLEEELLKV
                     QKLESIGVLAGGVAHDFNNLLTSIMGNISLSSLPDISDEKVKRSLLQALKACHKAKDL
                     STQLLTFSKGGALENRTVVSLEKVIRETANFTMSGSNIDFDFIVDDKLWTVEIDEGQI
                     SQVISNMLINAAQAMTNHGKITIRIENAEARKEKGVPLDDGKYVKTTITDEGVGIPKE
                     YLSRIFDPYFTTKQSGSGLGLSTSYSIIKNHGGYITVESELHVGTKFVIYLPAKGYSV
                     SAEASIESRIKKGRGRVLIMDDKMEVREAAGEMLRHMGYEVEFAKDGSEALKLYKRAK
                     MESVPFDTVIMDLTVPGGMGGKEAIEKLLVFDPGVKAIVSSGYSSDPILEKYQKYGFK
                     GVLPKPYEMGKLSDVLYGVLAEGN"
     gene            complement(5548..7371)
                     /locus_tag="VFJ67_01075"
     CDS             complement(5548..7371)
                     /locus_tag="VFJ67_01075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET7289000.1"
                     /translation="MASPAKTIKSGSEGVAWDLSDLYSGLNDSRLERDTNDLIKKSAA
                     FEKKYRGKINSKTINAPTLLKAVSELESISEQVGKLLSFAYLIFAADTAKPEHGAFLQ
                     KIQEKATEARKYLMFFELEWVALNDSKARKLIGDRKLSHYRHFLEQERKYKPHRLSEP
                     EEKILDEKANTGSRAFKRLFDEVLNNIRFKVRLDGKVRQLSETETLALLYDPDRTKRK
                     AAAEGLTEGLLANSHVLTFIFNTLVQDHAVSDRLRSFSYPMESRHLDNEIDRDTVDAL
                     ISSCEKNYGMVTKYYKLKKKLLGLKKFCDYDRYAPIFSDAKTIRYGEGKEIILSSFDV
                     FSPKMSEIAGKFFEKNWIDAETRDGKRGGAFSHGTVPSAHPYVFMNYTGRLRDVMTLA
                     HELGHGVHQYLSRKQGYFQSNTPLTTAETASVFAEMLVFHKLTETVKDPRTRLSLVCS
                     KLEDIFATVFRQVVLTRFEESLHTARRERGELRQEDIDKLWVKANEPMFGDSVELTEN
                     YSHWWLYIPHFIHSPFYCYAYSFGELLVLALYHKYLNEGNEFVPRYIELLSSGGSDAP
                     DKLLERVGVNIKDPGFWQGGLDLLKDMVDDALGLAEMKGIS"
     gene            complement(7464..7925)
                     /locus_tag="VFJ67_01080"
     CDS             complement(7464..7925)
                     /locus_tag="VFJ67_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET7289001.1"
                     /translation="MGLQEKIPQDLKEALRGKRELELSVLRMLQSSIKNKEIEKRKKE
                     LSDEEVIEVVGAEVKKRKEAAAEFEKVNRQDAADREKAEIEILMKYMPAQMSEDEIRA
                     QVKKAVTESGADGIKDLGKVMKIIIPALKGKADGAVVNRIVREELEKPAEG"
     gene            complement(8024..8554)
                     /gene="hpt"
                     /locus_tag="VFJ67_01085"
     CDS             complement(8024..8554)
                     /gene="hpt"
                     /locus_tag="VFJ67_01085"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET7289002.1"
                     /translation="MITEIRGRKLRPLISAADIKTKVGEMGKRIAEDYRDKKPVFIGI
                     LKGGFIFLADLVREVDFNVEIDFVKASSYKEGMEPGEIELVTDVTTPLKGRHVLLVED
                     MLDTGITLHFLKSVILSKEPASLKLCALIDKKERRQIDIEADYTGFDIGEGFIVGYGT
                     DCGEMGRNLPGVYVLE"
     gene            8712..>9542
                     /gene="tyrS"
                     /locus_tag="VFJ67_01090"
     CDS             8712..>9542
                     /gene="tyrS"
                     /locus_tag="VFJ67_01090"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7289003.1"
                     /translation="MKFIDPGEQLRIIKRGTEEIISEPELLEKLKKSRDTGRPLRVKA
                     GFDPTAPDLHLGHCVLLRKLREFQDLGHTVLFLIGDFTAMIGDPSGRSETRPPLTKAQ
                     VEENAGTYERQVFRILDREKTEVMFNSSWLGRLTGEDMIRLSSLETVARMLERDDFSK
                     RYKSGVPISINEFLYPLIQAYDSVSMKSDVEFGGTDQKFNILLGRGVQKHFEQEPQVA
                     VLFPILEGLDGVRKMSKSLDNYIAVEDTAVDMYGKIMSVPDDLMWRYYLLLTSRTEAQ
                     I"
BASE COUNT         2205 a         2530 c         2497 g         2310 t
ORIGIN      
        1 tagttctgtt ttctattagt tctgtttttc ggtttcgacg ggcgtggccg acccctttat
       61 atcgctcgta ttaaccacct tgtttatacc gtccgatttc acgaccgaaa gcccgttcag
      121 acggagcacg gtgtcgaaca gggacaatac gtcctccttc ctgagcccgc cgccgggggt
      181 tacgatgctt atctcattcg ggtgcacgga gccgtcgatg acgtaagtct cgtcgtttat
      241 ctcgctcacg gccagaatca ggtcggtaat atttatactg ccctgaagat tgacgattcc
      301 gtcttcggga agggagccgt cttctccggt atcttcactg tcctgcccgg tctgtccctg
      361 gacttccctc ctctgcgagt aatcgtcgag aggcgcggta actatatcca gtagaaaaag
      421 cccgattacg aaaatcagcg ttctgtatat catttcgaat tacggaatta acggatgcaa
      481 agaagattaa tgagtgaaaa tcctaacacg gattttaacc ggcgggaagt cttccggccc
      541 tatgtcgcta tttctttgat gcgtttcagc cttgccgtcc tcttgtcttt cctctcctgg
      601 gctttcttcc agagcctttt ctcttccgga gtttccgggc tgaacgcggg cacctgtttc
      661 gggagcccgt cgtctcccac gtgcacgtag gtgaggaatg ccgtacaggt atgcctctgc
      721 tcgcctgtca ggatattctc cgaaaggacc ttgataccga cttcgaggga cgaattaccg
      781 acgtaagtaa tgcctgactt gaatatggtt atatcaccga ctttgatggg cgagtagaaa
      841 aagagctcgt cgagcgagcc cgtcacgagg acgtccttga cgaacctcat cgcgagtatc
      901 gcggcagtct cgtctatatc gagcatgagc ttgcctacag acatgaagct gccgtaaaaa
      961 ctgtcctcgg ggaggaccac tttcgtcgtt tcgagcctca tcctcgttat ctcggtctcg
     1021 tcgatgatat tcttcgcctg ctcgtcacgt ataccgattt tcggtatcct ctggtccctc
     1081 cttttccgtg cttcctcgta gagggccttc tcgtcgtccg cgggctcgat ctgttcgttt
     1141 atgcgcctcg ggttcccgtc cccgtccacg gctataaagg cgaggtgggc ggaggtcgta
     1201 agcttcttct ccctggtttc gagattttcg gaatgtaccg tgacgcctat ctccatcgag
     1261 ctcgagccga tgtattcgat cgagctgtcg agcataacga tatcccccac cttgaccggg
     1321 tttatgaaat ctatgctgtc ggtcgcgccg agcacggtta tgcccctgga gatgcgcgac
     1381 gagttgatgc tccccgcgag catgatccag tccatcagcc tgcccccgtg gagcgtgccg
     1441 tggatgttcg cgtgctcggg aaaaacgaac tgtaccatct ggtgtctcgt gtcttttatc
     1501 gaaggcatct cattttcccg ctccggggcc ttttggagct cggactatta taatacaagg
     1561 aatccgataa attccagact cgtcatatca tcccgcgccc tctccgtttc gggacgcggc
     1621 gtgtcgggcg gtcctgtatt accggaccgg atgccgcaat tgtttataaa attactgaac
     1681 gatgaggaat gactgagaaa tgtcggaggt agggacaaat attgtcccaa ttcctacaat
     1741 tcgatactta gggccactaa atgttcaaca atagtaacaa tattacagga tgttacgctc
     1801 gcctcaaaaa acgcgcgtat tggcatgttt tttgaattat tgtttataac tcgttagaca
     1861 aggagggatg atgacggacc ggttcgtctc cgagccggat acgaataatc atattcagcc
     1921 gttttaagac gcgatcaccc agcggctgcc gcaatcaact aaagaacatg gaacagaata
     1981 taaagattct actgattgag gataatccgg attatgcgca attgatcaag agaattctct
     2041 ccaagaagac gtcatgctct atggaagtac tgcattgcga tacattttca agcggctacg
     2101 aaaaggcttc ctcgggcggc atagacctca tacttctcga cctcgacctc ccggacagcg
     2161 gtaacataaa tacgctcctc agcataagcg aggtcacgga cgtccctatt atagtcctta
     2221 cgggcacaga cgacgaatcc ctggcgctga aggccgtgca gatgggcgcc caggactatc
     2281 tcatgaaggg gcaggtggac gcgaggctgc tcgtgcgttc gataatgtac gcgatagagc
     2341 ggaagaaagc cgaagacgcc gttaagcaga ccgaagagag gttcaaactc ctcatagaga
     2401 atgcgctcga ccttattgaa atgctggacg ttgacggcac gatgaaattc gtaagcccat
     2461 cccataaacg cgttctgggt tacgagaacg acgatctgat cggaaggagg atattcgagt
     2521 tcgtccatcc cgaggacttg cccggggtca tagacgtgtt caacgagacc ctccagaacc
     2581 cggaccgtcc ttactccgcg gagttccgcg tcaggcacaa ggagggttac tggatcacgc
     2641 tcgaatcgat agggaagcat tgtccgcccg agctgagcag tatcggagtc atcgtgaacc
     2701 tgcgcgatat tacagaacgc aaaaagatgg aagagcggct acgcagtctc tcgataaccg
     2761 acgagctcac cggcctctat aacaggcgcg gatttctgac cttcgccgac caccatatca
     2821 aggcggcgga aaggaggaaa gagtcggtgc tgctgatcct gatcgatctg gacgggctca
     2881 agcagataaa cgacgcgtac gggcacaata tcggcgacca ggcgcttatg gacgccgctc
     2941 aggtgatcaa gcagacgttc aggagcacgg acgttacggc caggataagc ggagacgaat
     3001 tcgtagtcgt aacgaccgat tccgacggga acggacagga gtcgataaag agacggatag
     3061 aggaaaaact gaaagaatat aattccaagg gcataaagcc ctatcacctg tccttcagct
     3121 atggtgtcgc gacgttcgat ccgcaggacc cgtcgtcgat agacaggctg ctcgtgaagg
     3181 ccgatgagct gatgtacctc aataaaaaca acaaatacgt cgatacgaat gagaacgttc
     3241 tcaatctgaa caggtccaaa aaataattac acgatccatt tttctcgctg accggattat
     3301 aaagagccgc ggtcaccggg ccgaaagact ttcggagaca ggctcgcatg ccgatttttg
     3361 acaaagcttt gttgtcttaa tccgtgaatt ttacgcaatt tgtcagtaga gtcctgggta
     3421 atttttgcgc aatcagcaaa ataatattta aagacaatta attatgatgt ccgttgatga
     3481 tggtatgttt tttgcactta ttatttacaa ggttcaagct gattccgaaa aaatctgaag
     3541 gccggatcag ccgcatttcg gaaaacaaat gtctgcccgt ctgattaaaa cattactggt
     3601 tgaggataat gacagttatt ccagactggt cgttaaaatg ctcggagggg ccgacaacag
     3661 cggattcgac atcgaatccg ttgataccct cgccaaaggg ataaccgttc tcgaagaaaa
     3721 agaattcgat ttggttctgc tcgacctcca gcttccggac agcgaagggc tcaggacttt
     3781 taccgggatc cacgaacgtc atcccggaat ccccgtgata gtcctcactg gcaccagcga
     3841 tgaagacacc gcggtcaaag cggtccagct cggagcccag gactatctcg tgaagggcga
     3901 gatggaaata ctgcagctct cgcggtcgat aaaatatgcg ctcgagaggc agagggaaat
     3961 aaataagagg aagctcacag agcaggccct cttcgaggag aaagagcgtc tcgcggttac
     4021 gctggcctcg atcggtgacg gggtaatcac gaccgatacg gaaggaaagg taacgcttat
     4081 aaaccgggtg gcggagcgtt tgacgggctg gtctcacgct gaggctgcgg gaagattcat
     4141 cgaagaagtg ttcttcatag tcaacgtcga aacgggggag aaaaaagaga acccggtgag
     4201 cagggctatt aacgccaaca atgtcgtggg tcttgagagc ggtacagtgc ttataaccaa
     4261 gggcagagaa gggtgccggt atgtttcggc gagcagctct ccgatccgcg acagggacgg
     4321 cataattata ggcgtcgtgc tcgtcttcag ggacataacg gagctgaaga agctcgaaga
     4381 agagctcctg aaagttcaga agctggaatc gataggagtg ctggcgggcg gagtggctca
     4441 cgatttcaat aacctgctca cctcaatcat ggggaatatc tccctgagct cgctcccgga
     4501 tatatcggac gaaaaagtca aacggagcct cttgcaggcg ctcaaggcat gtcataaggc
     4561 aaaggacctg tccacccagc tgctcacctt ttcaaagggc ggagcgcttg agaacaggac
     4621 cgtcgtgtcg ctcgaaaagg tcataaggga gaccgctaat tttactatga gcggctcgaa
     4681 tatagatttc gattttatag tggacgacaa gctctggacc gtcgagatag acgagggaca
     4741 gataagccag gtcataagca atatgctgat aaacgccgct caggcgatga cgaaccacgg
     4801 gaaaataacc atcaggatcg agaacgcgga ggccaggaaa gaaaagggcg tccctctcga
     4861 tgacgggaaa tacgtaaaga ccactataac ggacgagggt gtcgggatac caaaagagta
     4921 tctgtccagg atcttcgatc catactttac gaccaagcag tcggggagcg gtctcgggct
     4981 ctcgacctcg tattcgataa tcaagaacca cgggggctat ataaccgtcg agtccgagct
     5041 ccacgtgggg acgaaattcg ttatatacct gcctgccaag ggatattcgg tgagcgccga
     5101 ggcgagtatc gagagtcgga taaagaaggg aaggggcagg gtccttatta tggacgacaa
     5161 gatggaggtc agggaagccg cgggcgaaat gctgaggcac atgggttacg aggtcgaatt
     5221 cgcgaaggac ggaagcgagg cgttaaagct ctacaagagg gcgaagatgg aaagcgtgcc
     5281 gttcgatacg gtgataatgg acctgactgt gccgggaggc atggggggca aggaggcgat
     5341 cgagaagctt ctcgttttcg accccggagt gaaagccata gtttcgagcg gttattcgag
     5401 cgacccgata ctggaaaagt accaaaagta cggattcaaa ggcgtgctcc ccaagcccta
     5461 cgagatgggc aaattgagcg acgtcctcta cggcgtcctc gccgagggta actgagcagt
     5521 ccgtgaacct ctgatccggt ttaactttca ggaaatcccc ttcatctccg caagtcccag
     5581 agcgtcatcg accatatctt tcaataggtc taacccgccc tgccagaacc cggggtcctt
     5641 gatattcacg cccacgcgtt cgagcagttt gtctggagca tcgcttccgc ccgaagagag
     5701 cagctcaatg tagcgcggta cgaattcgtt cccttcgttt aaatacttat gataaagagc
     5761 gagcacgagc agctcaccga aagagtacgc ataacaatag aaaggcgagt gtatgaagtg
     5821 gggtatgtat agccaccagt ggctgtagtt ctcggtcagc tcgacagaat ccccgaacat
     5881 gggctcgttc gccttcaccc agagcttgtc gatatcttcc tgcctgagct ctcccctctc
     5941 ccttctcgcc gtgtgcaggc tttcctcgaa cctcgtcagc accacctggc ggaaaacggt
     6001 cgcgaatata tcctcgagct tgctgcatac gagggaaagc ctcgtcctcg ggtctttcac
     6061 cgtctccgtc agcttgtgga atacgagcat ctccgcgaat acgctagccg tctcggccgt
     6121 cgtgagagga gtgttcgact ggaaataccc ctgctttctc gaaaggtact ggtgcacgcc
     6181 gtgcccgagc tcgtgcgcga gcgtcatcac gtccctaagc ctccccgtgt agttcatgaa
     6241 gacgtaaggg tgcgcgctcg ggaccgtacc gtggctgaaa gccccgcccc gcttcccgtc
     6301 cctcgtctcg gcgtctatcc agttcttctc gaaaaatttc ccggctattt ccgacatctt
     6361 cggagagaaa acatcgaacg acgaaaggat tatctcctta ccttccccgt acctgatggt
     6421 cttcgcatcg gagaagatag gcgcgtagcg gtcgtaatcg cagaacttct tgagacccag
     6481 gagcttcttt ttgagcttgt agtatttcgt caccatgccg taattttttt cacacgacga
     6541 gatgagcgcg tccaccgtgt ccctgtctat ctcgttgtcc aggtgcctcg actccatcgg
     6601 atacgagaac gagcgcaggc ggtcgctcac cgcatggtcc tgaacgagcg tgttgaatat
     6661 gaacgtcagg acgtgagagt tcgcgagcag cccttccgtg agcccctcgg ctgcagcctt
     6721 cctcttcgta cggtcagggt cgtaaaggag agcgagcgtc tcggtctcgc tgagctgcct
     6781 gacctttccg tcgagcctga ccttgaacct tatattgttc aggacctcgt cgaacaggcg
     6841 cttgaacgcc ctcgatccgg tattcgcctt ctcgtccaga atcttttcct cgggctcgct
     6901 cagcctgtgc ggcttatact tacgttcctg ctcgaggaaa tgacggtaat gcgagagctt
     6961 cctgtcgcct atgagcttcc gggcttttga gtcgttcaat gccacccact cgagctcgaa
     7021 gaacatgaga tatttcctcg cctcggtcgc cttttcctgt atcttttgca aaaacgcgcc
     7081 gtgctcaggc ttcgcggtgt cggcggcgaa aatcaggtac gcgaatgaaa gcagcttccc
     7141 tacctgctcg ctgatggatt cgagctcgct cacggctttc agcagggtag gggcgtttat
     7201 agtcttcgag tttatctttc ccctgtattt tttctcgaag gcggctgatt tttttatcag
     7261 gtcgttcgtg tctctctcta accggctgtc gttaaggccg gagtagaggt cggataggtc
     7321 ccatgcgacg ccctccgagc ctgactttat agtttttgct ggacttgcca tttgcatttc
     7381 tcctcttcgg tcgtttccgg atttggatac tggttacaat agtagtttcg agtctcggtg
     7441 attgtttgat gccgcggaaa aaatcatccc tctgccggtt tctctaattc ttccctgact
     7501 atcctgttta caaccgcgcc gtcagccttt cccttaaggg cgggaattat tatcttcatc
     7561 accttcccga ggtcctttat cccgtccgcg cccgattcgg tgacggcttt cttcacctga
     7621 gccctgatct cgtcctcgct catctgcgcg ggcatgtatt tcatgagtat ttctatctcg
     7681 gccttctccc tgtcggcggc atcctgcctg ttcactttct cgaattccgc cgcggcctct
     7741 tttctttttt tcacctcggc gcccacgact tcgataactt cctcgtccga gagctccttc
     7801 ttgcgcttct cgatctcctt gttcttgatc gacgactgca gcatcctgag cacggatagc
     7861 tcgagctctc ttttacccct gagcgcttct tttaaatcct gtggaatctt ttcctgtaat
     7921 cccatattcg tctccgccgg tttttgattt attttaaccg ttttgaggcg gttattttac
     7981 ttcatggttt cgtgcccggg cgtcacgtag tgcccgtgcc gtgctattcg agcacgtaaa
     8041 ctcccggcag gttcctcccc atctcgccgc agtcggtgcc gtaccctact ataaagccct
     8101 ccccgatgtc gaagcccgta taatcggcct ctatgtcgat ctgtctcctc tctttcttgt
     8161 caatgagcgc gcagagtttt agtgacgccg gctccttcga aagtatcacc gattttaaga
     8221 aatggagcgt gatccccgtg tccagcatgt cttccacgag caggacgtgc ctgcccttaa
     8281 gcggggtcgt gacgtccgtc acgagctcta tctctcccgg ctccataccc tctttatatg
     8341 aagacgcctt tacgaaatcg atctcgacgt taaaatcgac ctccctcacg aggtccgcga
     8401 ggaatatgaa cccgcccttg agtatgccta tgaagaccgg tttcttgtcc ctgtaatcct
     8461 ccgcgatccg cttccccatc tcccccactt ttgtctttat atcggccgcc gaaatgagcg
     8521 gcctgagctt cctgccccgg atttccgtta tcatgcgcag atattctatc caaatctttc
     8581 ggttataaac agccgtgtaa atgcgctttg tgcgcccggc aaaatatcgc cattgactaa
     8641 ctattgagat attctatatt tttgagcggc cgcatcgact gaaatattgc gaaaataggg
     8701 gaacttataa agtgaaattt atagatccag gggagcagct ccggattatc aagcgtggga
     8761 ccgaggaaat aatatcggag cccgagctcc tcgagaagct gaaaaaatcg agggatacgg
     8821 gcagacccct gagggttaaa gccggtttcg atcccactgc gcccgatctt cacctcggac
     8881 actgcgtcct gctgaggaag ctccgtgaat tccaggacct ggggcatacg gtgcttttcc
     8941 ttataggaga ttttacggcc atgatcggcg acccttcggg cagatccgag accaggcctc
     9001 cccttacgaa ggcccaggtc gaggagaacg ccgggactta cgaaagacag gtcttcagga
     9061 tactcgacag ggaaaaaacg gaggtcatgt ttaattcctc atggctcggc cggctgaccg
     9121 gtgaggacat gatcaggctg tcctctctcg agaccgtggc ccggatgctc gagagggacg
     9181 atttcagcaa gcgctacaaa tccggcgttc cgatctccat aaacgagttt ctctatcccc
     9241 tcattcaggc gtacgattcg gtcagcatga agtcggacgt cgagttcggc gggaccgacc
     9301 agaaattcaa catacttctc ggccgcgggg tgcagaagca tttcgagcag gagcctcagg
     9361 tcgcggtatt gttcccgata ctggaagggt tagacggagt gaggaagatg tccaagtcac
     9421 tcgacaacta catagctgtc gaggacacag ccgttgatat gtacgggaag ataatgtccg
     9481 tgcccgacga cctcatgtgg cggtattacc tgctcctgac ctctaggacc gaggcacaga
     9541 ta
//
LOCUS       DASSJH010000050         5117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_444271, whole genome shotgun sequence.
ACCESSION   DASSJH010000050 DASSJH010000000
VERSION     DASSJH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444271"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFJ67_01095"
     CDS             <1..754
                     /locus_tag="VFJ67_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7289004.1"
                     /translation="SIIIGKLLARDLGIKEAGKKVTLVSPSGGIYTLKVVDFFNSGIT
                     SLDQTRVYMNLRTLQAILDKPNQVNELIIKVKEVDQAEPFAREMSEDTGYYAESWQRA
                     FSNFLQIFKMQNWITYMMVFAILIVSAFGIFNIMMMTVLEKKRDIAILKAIGYEDGEI
                     TRIFVFQGLLVGLIGALFGCLLGYLIEVWLAHLKFDIVGIIRASGFVLDRRPRYFVLG
                     FVFAMIFAFFASFYPSWRASRLFPVDIFRSSV"
     gene            751..1413
                     /locus_tag="VFJ67_01100"
     CDS             751..1413
                     /locus_tag="VFJ67_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7289005.1"
                     /translation="MSVILEGRDIKKSIGENEILKGISIQISAGDFMSIVGTSGSGKS
                     SLMYILGLLDAPTSGDVMIDGRKIDFRDAKLLAGIRNRKIGFVFQFHYLITELTAYEN
                     VLIPMLKNGIKDGEGAKARSYALIESLGLKGKEDRKPYQLSGGEQQRVAIARALANDP
                     EIVMADEPTGNLDSKNSAMVMDVFLELNEKGRSILMITHEMYLARETKSVLEIKDGLI
                     VP"
     gene            1440..2465
                     /locus_tag="VFJ67_01105"
     CDS             1440..2465
                     /locus_tag="VFJ67_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET7289006.1"
                     /translation="MKAMLLEKIVSMSGTDEPLRLADVPKPAPGPGEVLIRLSACGVC
                     HTELDEIEGRTPPPRLPVIPGHQAVGVVEEAGGRVKRLKKGDRVGVAWIFSSCGKCRY
                     CLEGNDNLCPEFRATGRDADGGYAGYMTVGEDYAFEIPGNLTDSEAAPMLCAGAIGYR
                     SLRLTGIEDGERLGLTGFGASGHLVLKLARFMYPKSGIYVFARSESEREFARELGAVW
                     TGGTEETPPEMLDAIIDTTPVWKPVVEALAKLAPEGRLVINAIRKEDADKDYLLRLDY
                     PSHLWMEKEVKSIANVSRRDVREFLALAGKAGLKPIVEEYALEDANRALAELKAGGIK
                     GAKVLRT"
     gene            complement(2502..3185)
                     /locus_tag="VFJ67_01110"
     CDS             complement(2502..3185)
                     /locus_tag="VFJ67_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET7289007.1"
                     /translation="MYENAIMKISRSIFPVFYRIHINGITTVGLSGTAFFINDLGHFI
                     TATHVTAEVPSGAKLLYAGNLPHAALDHPVEIEEIYKNELKYIYIGKVPKSPLPGLKF
                     TGYESPPGKAVCLGGYPLAEMIINPDESVNLSNVRQYWKPTMIIDSIEAAINHGTQTG
                     FITEHSSLNGMSGGPVFDSDGEVCGIDVTTYLREIPEPNGGKTFVRNGVVIGTLKMRD
                     LIPDGIPLS"
     gene            complement(3242..4849)
                     /locus_tag="VFJ67_01115"
     CDS             complement(3242..4849)
                     /locus_tag="VFJ67_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7289008.1"
                     /translation="MRKLKIGIVDFITKAPNSSLWARFMHANLAGIMPQVVASWCESL
                     GHEVTLFLYTGVEDLYKDVPKDIDIVFISAFTQSAIHAYALSNKYRHEGVVTVLGGPH
                     ARCYPEDAQKYFDYVLGLTDKETCRDVLEDFSLHNEQGMYMSALKQPGTLPGVRERWK
                     FISQALDKAPWIKVVPMIGSVGCPYACSFCIDADQPYQTLGFDVIKDDLRFLLTKFKR
                     PLVSWHDPNFGVRFDDYMETLEEAVPPNSIDFIAESSLSLLSEPHLKRLRQNGFKAVL
                     PGIESWNGMGNKSKTGSKQGQDKLNAVSEHVNMILRYVPYIQTNFVLGLDCDEGPESF
                     ELTKKFVDMSPAAFPAFPLLTSFGKAAPDNIGYQGEERVLPFPFHFLNNNHAMNVKPK
                     TSTWPEFYDRVIDLVEHSFSWPSIINRFRNTNSRIPAWMNLMRAVSTEGFGRMRFYRK
                     IRRLLDKDRAFRDYFEGETSVLPEFYMNMVRKDLGKLWEWLPEGALYHDQNAYFKQEQ
                     EKKVIEPDVIKPIEIKNGLAGVRIMTG"
BASE COUNT         1166 a         1325 c         1492 g         1134 t
ORIGIN      
        1 cagcatcatt atcggaaagc tcctcgcgag ggacctcgga ataaaagagg cggggaagaa
       61 ggtgacgctc gtatcgccga gcggaggcat atacacgctc aaggtcgtcg acttcttcaa
      121 ctcaggcatc acgagcctcg atcagacgag ggtctacatg aacctccgca cgctacaggc
      181 gatactcgat aagccgaatc aggtgaacga gctgataatc aaggtgaagg aagtcgacca
      241 ggccgagccc ttcgcccgag agatgtccga agacacgggg tattacgccg aaagctggca
      301 gagggccttc agcaacttcc tccagatatt caagatgcag aactggatca cctacatgat
      361 ggtgttcgcg atactgatag tatcggcgtt cggcatcttc aacatcatga tgatgactgt
      421 gctcgagaag aagagagaca tagctatact caaggcaata gggtacgagg acggtgagat
      481 aacgaggata ttcgttttcc aggggctcct cgtgggcctg ataggggcgc tttttggatg
      541 cctcctcggt tatctcatcg aggtatggct cgcgcattta aaattcgaca tcgtcgggat
      601 aatcagggcg agcgggttcg tgctcgacag aaggcccagg tatttcgtcc tcggcttcgt
      661 attcgcgatg atattcgcct tcttcgcctc gttctacccg tcctggaggg cgtcgcgcct
      721 cttcccggtc gatatattcc ggtccagcgt atgagcgtta tcctcgaagg cagggacata
      781 aagaaatcca taggcgagaa cgagatcctg aaagggatat cgatacagat atcggccggg
      841 gatttcatga gcatcgtcgg gacttcaggc tcgggaaaaa gctcgctgat gtatattctc
      901 ggactcctcg acgctccgac ctccggggac gtaatgatcg acggcaggaa gatcgacttc
      961 cgggacgcga agctccttgc cggaataagg aacaggaaga tcgggttcgt gttccagttc
     1021 cattacctta taaccgagct gaccgcctac gagaacgtgc ttatccccat gctgaagaac
     1081 ggtataaagg acggggaggg cgcgaaagcg aggtcttacg ctctcataga gagcctcggc
     1141 ctcaagggca aggaagacag gaagccttac cagctctcgg gaggggagca gcagagggtc
     1201 gcgatagcgc gggccctcgc gaacgacccg gaaatagtga tggcggacga gcccacggga
     1261 aacctcgact caaaaaactc ggccatggtc atggacgtat tcctcgagct gaacgaaaag
     1321 gggaggtcga tactgatgat cacgcacgag atgtatctcg cccgggagac gaagagcgtg
     1381 ctcgagataa aggacgggct catcgtgccg tgacaggcgc gcaagggaag gaaaatggaa
     1441 tgaaggccat gctgctcgaa aaaatagtct ccatgtccgg aacggacgag ccgctccgac
     1501 tcgccgacgt tccaaaaccc gctccgggac cgggtgaagt gcttatcagg ctcagcgcct
     1561 gcggcgtctg ccatacggag ctggacgaga tagaggggag gacgccccct ccgaggctgc
     1621 ctgttatacc cggccatcag gcggtagggg tcgtcgagga agcaggcggt agggtaaagc
     1681 gcctgaagaa gggcgacagg gtaggggtcg cgtggatatt ctcctcgtgc ggaaaatgcc
     1741 ggtactgcct cgaagggaac gacaacctct gcccggaatt cagggccacg gggagggacg
     1801 ccgacggcgg ctacgccggg tacatgacgg tcggcgagga ctacgcgttc gaaattcccg
     1861 gaaacctcac ggattcagaa gctgcgccca tgctctgcgc gggggcgata gggtacaggt
     1921 cgctcaggct tacggggata gaggacggcg agaggctcgg cctcacggga ttcggcgctt
     1981 ccgggcacct cgtgctaaag cttgcgcgtt tcatgtatcc gaagtccgga atatacgtct
     2041 tcgcgaggag cgaatccgaa agggagttcg cccgcgagct gggcgctgta tggacgggcg
     2101 gcacggaaga aactcctccc gaaatgcttg atgcgataat agataccacg cccgtgtgga
     2161 agcccgtcgt cgaggcgcta gcgaaactgg cccccgaagg aaggctcgtc ataaacgcca
     2221 taaggaagga ggatgccgac aaggattatc tgctcaggct cgattacccc tctcacctct
     2281 ggatggagaa ggaagtaaaa tccatcgcga acgtgagcag gagggacgtg agagagttcc
     2341 tcgcgctcgc cgggaaagcc ggtcttaaac ccattgtcga ggaatacgcg ctcgaagacg
     2401 cgaacagggc gcttgcagag ctgaaagcgg gcggtataaa aggggcgaag gtgctgagga
     2461 cgtgaccttt gtatatcgta tgcgctccgg ggcgggtgcc gtcaggagag cggtatgccg
     2521 tcggggataa ggtccctcat ttttaacgtt cctatgacga cgccgtttct tacgaatgtc
     2581 tttccgccgt tcggctcggg tatttcccgc aggtaagtgg tgacatctat accgcatact
     2641 tccccgtcgc tatcgaagac gggtcccccg ctcataccgt taagcgaaga gtgctccgtg
     2701 atgaaccccg tctgcgtccc gtgattgatc gcggcttcga tgctgtcgat gatcatggtc
     2761 ggcttccagt attgtctgac gttgcttaaa ttcaccgact cgtccgggtt tataatcatc
     2821 tcggccagcg gatagccgcc gagacatacc gccttgccgg ggggcgactc atacccggtg
     2881 aatttgagtc cggggagggg ggatttgggc accttgccga tgtagatgta tttcaattcg
     2941 ttcttgtaga tctcttcgat ttcgacgggg tggtcgagag cggcgtgggg gaggttgccg
     3001 gcgtataata acttcgcgcc cgacggcact tccgcagtca catgcgtcgc cgttataaaa
     3061 tgcccgaggt cgtttatgaa gaaagcggtg ccggagaggc cgacggttgt aatcccgttt
     3121 atatggatcc tgtaaaatac aggaaatatc gacctggata ttttcattat cgcattttcg
     3181 tacatctttt attctctctt gttgctatgt atctaatttt tacttactga acctgacccg
     3241 cttacccggt cattatccgc actccggcaa gcccgttctt gatctctatc ggctttatta
     3301 catcaggctc gattaccttc ttctcctgct cctgcttgaa atatgcgttc tgatcgtgat
     3361 agagagcccc ttccggcagc cattcccaga gcttgccgag gtctttcctg accatgttca
     3421 tgtagaactc gggcagtacg ctcgtctccc cttcgaaata gtccctgaac gccctgtctt
     3481 tatcgaggag ccgccttatc ttcctgtaga acctcatcct cccgaagcct tcggtcgata
     3541 cggcgcgcat aaggttcatc cacgcgggta tcctagaatt agtgttgcgg aaacgattta
     3601 tgatcgaggg ccatgagaac gagtgctcga caagatctat gaccctgtcg tagaattcgg
     3661 gccacgtgct cgtcttgggc tttacgttca tggcgtggtt gttgttgaga aaatggaacg
     3721 ggaacgggag gacgcgctcc tcgccttggt agccaatgtt atcgggagcc gccttgccga
     3781 aagatgtgag tagcgggaaa gccggaaaag ccgcaggtga catatccacg aatttctttg
     3841 tcagctcgaa cgactcgggg ccctcgtcgc agtcgagacc gagaacgaaa ttagtctgga
     3901 tgtaggggac ataccggagg atcatattca cgtgctcgga gaccgcgttt agcttgtcct
     3961 gtccctgctt gctccctgtc ttggatttat tgcccatgcc gttccacgac tctattccgg
     4021 ggagcaccgc cttgaatccg ttctgtctga gcctcttcaa gtgaggctcg gaaagcagtg
     4081 aaaggctgct ctccgcgatg aagtcaatgc tgttgggcgg aaccgcctct tcgagagttt
     4141 ccatgtagtc gtcgaacctc accccgaagt tcggatcgtg ccagctgacg agcgggcgtt
     4201 tgaacttcgt caggaggaac ctgaggtcgt cctttattac gtcgaacccg agtgtctgat
     4261 agggctggtc ggcgtcgatg cagaagctgc acgcgtaagg acagccgacg ctgccgatca
     4321 tcggcaccac tttgatccac ggagccttgt cgagagcctg ggaaatgaat ttccacctct
     4381 cccttacgcc gggcagtgtg ccgggctgtt tcagggcgga catatacatg ccctgttcat
     4441 tgtgaagtga aaaatcttcg agaacgtcgc ggcaagtttc tttatcagtg agcccgagca
     4501 cgtagtcgaa gtatttctgc gcgtcctcgg gatagcagcg cgcgtgaggc cctccgagaa
     4561 ccgtgacgac cccttcgtgc ctgtacttgt tgctgagcgc gtaagcatga atggcggact
     4621 gtgtgaacgc gcttatgaat acgatgtcga tgtccttcgg gacatcttta tagaggtcct
     4681 cgacgcccgt gtataaaaac agtgtgacct cgtggccgag actctcgcac caggacgcca
     4741 ctacctgcgg catgataccc gccaggttag cgtgcatgaa tcgcgcccag agtgagctgt
     4801 tgggcgcttt ggttatgaag tccacaattc cgattttcag cttccgcata aatcacctca
     4861 ttttaatccc tgttttataa agaacggtcg acgctatacg gcgggtcgag cgaaacgctt
     4921 tccgacaagc cctcccggca cttatcccgg caggaaagaa ataagtaatg gaaatcaggc
     4981 cattgcaaac cgcataaaag ttaaactgat ccccttaatg gccttgagag gagtaatcat
     5041 aagaaataat gataaaggga tgaattgaaa aaatgtctgg atggttgtta aatcgttcaa
     5101 atccagtggg gtcgtct
//
LOCUS       DASSJH010000051         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1418394, whole genome shotgun sequence.
ACCESSION   DASSJH010000051 DASSJH010000000
VERSION     DASSJH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418394"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /gene="ftsY"
                     /locus_tag="VFJ67_01120"
     CDS             <1..1010
                     /gene="ftsY"
                     /locus_tag="VFJ67_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET7289009.1"
                     /translation="EEIVQETPAEEPQAEVAPQEEVAETKEGLFARLRKGLSKTHAGL
                     FGRLGEVLSKKEIDESTWDDFEETLIMADIGVGTTMKLRGKIEASVEKKSLRDGDAIT
                     GALKEEILRILKTAEGTPISISSKPFVIMVAGVNGVGKTTTIGKLANKLKKEGLSVMV
                     AAGDTFRAAATEQLEIWSKRVGSDFIKGQSGADPSAVAFDAVKAASARGTDVLIIDTA
                     GRLHTKENLMDEIKKMKRIISRELEKAPNETLLVLDATTGQNAIQQAKIFNEALDVTG
                     IVLTKLDGTAKGGVIVAIADELNIPVKYIGIGESLSDLREFNAEDFVEALFYSGEETL
                     H"
     gene            1046..2167
                     /gene="ribD"
                     /locus_tag="VFJ67_01125"
     CDS             1046..2167
                     /gene="ribD"
                     /locus_tag="VFJ67_01125"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET7289010.1"
                     /translation="MNGTIKEAHEKFMSLAIRLAGKAEGMTSPNPLVGAVLVKRGQII
                     GKGYHRKAGLPHAEIEAFINAEKNGHKVKGSTLYVSLEPCCHREKKTPPCTDAIIGKG
                     VSEVIVGALDPNPRVSGNGVKSLEDAGIRVTAGVLEKKSRELNEAFNKYITTGTPFVT
                     LKLAATLDGKIATRTGDSKWIGSERQRKEGHRLRNLADAVMVGTGTVLKDDPRLNVRL
                     LKKPGRQPVPVVLDMKLKTPVSSNIFSAHENVIIVTGKSHDKNKRRKLEASGATVVAS
                     KAGRDGLLDLREVARRLGKMEITSLLIEGGSRTAALALKSGIVDKVVFFYAPRVIGAE
                     GLSMVGNMGKSRVSDSLKIERVKITKLGDEFMVEGYVSK"
     gene            2202..>2850
                     /gene="nadD"
                     /locus_tag="VFJ67_01130"
     CDS             2202..>2850
                     /gene="nadD"
                     /locus_tag="VFJ67_01130"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET7289011.1"
                     /translation="MTTRIKERAGLFGGTFDPVHLGHLRAAEEIRENLSLDKVYFIPA
                     ALAPHKKNANATPSLHRLEMLRLAVSDNPGFEVCDYELVKDAPSYTVETLRHLKKTNP
                     ESEYYFMVGNELFREIETWKEWRELFKLSNFAVITRPGYPGPAGEKLPLALENDFSYY
                     KEKENVIFYKDKNSSLIAFSRIMGLEVSSTEIRRCVKSGRSIKYLVPASVEEYILR"
BASE COUNT          781 a          690 c          887 g          492 t
ORIGIN      
        1 cggaagagat tgtgcaggaa actcccgcag aggagcctca agcggaggta gctccgcaag
       61 aagaggtcgc ggaaacgaaa gaggggctgt tcgcgagact caggaagggc ctctccaaaa
      121 cacatgcggg actcttcggc aggctcggag aggtgctgtc caagaaagag atagacgaga
      181 gcacgtggga cgatttcgag gagactctta taatggccga tataggggtc ggcaccacga
      241 tgaagctcag gggaaagata gaggcgagcg tcgagaagaa atcgctccgt gacggggacg
      301 ccataacggg tgcgctcaag gaagagatac tgaggatact gaagaccgcg gaggggactc
      361 cgataagcat aagctcaaag cccttcgtga taatggtcgc cggtgtgaac ggagtaggga
      421 aaactacaac tatcggaaag ctggctaata agctcaaaaa ggagggcttg agcgtaatgg
      481 tagccgcagg cgacacgttc agggcggccg caaccgagca gctagagata tggtcgaagc
      541 gcgtgggatc ggatttcata aaggggcaga gcggggccga cccttccgcg gtagcattcg
      601 acgctgtgaa agctgcgtcc gcgcgcggga ccgacgtgct catcatagac acggccggca
      661 ggctccatac gaaagagaac ctcatggacg agataaagaa aatgaagagg ataatttcgc
      721 gcgagctcga gaaggcgcct aacgagaccc tgctcgtgct cgacgccaca acgggacaga
      781 acgcgataca gcaggcgaag atattcaacg aggccctcga cgtgacgggc atcgtgctca
      841 cgaagcttga cggcacggcg aaggggggcg ttatcgtggc tatcgcggac gagctcaata
      901 taccggtcaa atatatcggg ataggggaga gcctgagcga cctccgggaa ttcaacgccg
      961 aggatttcgt cgaagccctc ttttattcag gcgaggaaac cctgcattga tgtatcctgc
     1021 atcatcggca tagagacaga cgcttatgaa cggtacgata aaagaggcgc acgaaaaatt
     1081 tatgtcgctc gcgataagac tcgccggaaa ggcggaggga atgacgagcc cgaaccctct
     1141 cgtcggagcg gtcctcgtaa agaggggaca gatcataggg aagggctatc acaggaaggc
     1201 ggggctcccg cacgcggaga tagaagcctt cataaacgct gagaaaaacg gacataaagt
     1261 gaagggctcg acgctctacg tgtcgctcga gccctgctgt cacagggaga agaagactcc
     1321 gccctgcacg gacgcgataa tagggaaagg ggtgagcgaa gtcatcgtag gcgcgctcga
     1381 ccctaacccc agggtgagcg ggaacggggt gaaatcgctc gaggacgcgg gtatacgcgt
     1441 gacggccggg gtgctggaga aaaaatcccg cgagctgaac gaggcattca acaaatacat
     1501 tacgacaggc acgccgttcg tgaccctcaa gctcgcggcg actctcgacg ggaagatcgc
     1561 cacgcgtaca ggcgattcca agtggatagg gagcgagagg cagagaaagg aaggccacag
     1621 gctcaggaac ctcgcagatg cggtaatggt cggcacaggg acggtgctca aggacgaccc
     1681 ccggctgaac gtaaggctgt taaagaagcc tggaagacag cccgtgccgg tcgtcctcga
     1741 tatgaagctg aagacgcccg taagctcgaa cattttttcg gcgcacgaaa acgtgattat
     1801 agttacgggg aaatcgcacg acaaaaacaa gcgcaggaag ctcgaagcct caggggctac
     1861 ggtggtcgcg tcgaaggcgg ggagggacgg gctcctggat ttgagggagg tcgcgcggcg
     1921 gctagggaag atggagataa cgagcctcct catcgaaggg ggcagcagga cggctgctct
     1981 cgcgcttaag agcggcatcg tggacaaggt cgtgtttttt tacgctcccc gggtcatagg
     2041 ggctgaagga ctgagcatgg tagggaatat gggcaaatcg cgcgtcagcg actcgctcaa
     2101 aatcgaaagg gtaaaaataa cgaagctagg cgatgaattc atggtcgaag ggtatgtgtc
     2161 taaataaaat tcaaaaagaa atgatatgat tgccccttac aatgactacc cggattaaag
     2221 agagagcggg actattcgga ggcacgttcg accccgtgca cctcggccac ctgcgcgcgg
     2281 ccgaagagat aagggagaac ctctctctcg acaaggtgta cttcataccc gccgccctcg
     2341 cccctcataa aaagaacgcg aacgccactc catctttaca cagactcgaa atgctgaggc
     2401 tggccgtctc ggataatccc ggcttcgagg tatgcgatta cgagctcgtg aaggatgcgc
     2461 cttcgtacac ggtcgagact ctcagacacc tgaaaaagac taatccggaa tcggaatact
     2521 actttatggt cggaaacgag ctcttcaggg aaatcgagac gtggaaggag tggagggagc
     2581 tgttcaagct gtctaacttc gcggttatca cgcggccagg ttatccgggc ccggccgggg
     2641 agaaactccc gcttgccctc gaaaacgatt ttagttatta taaagagaaa gaaaacgtga
     2701 tattctacaa agataagaac tcatcattaa tcgctttttc cagaataatg ggccttgaag
     2761 tttcctctac agaaatcagg cgctgcgtta agtcgggcag gtctataaag tacctcgtcc
     2821 cggcatccgt cgaagaatat attctgcggc
//
LOCUS       DASSJH010000052         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_445940, whole genome shotgun sequence.
ACCESSION   DASSJH010000052 DASSJH010000000
VERSION     DASSJH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445940"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..192)
                     /locus_tag="VFJ67_01135"
     CDS             complement(7..192)
                     /locus_tag="VFJ67_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289012.1"
                     /translation="MEIELELFAKDDDWEDDEEEEWDEDEEELWDEEDEDGLDEDEEW
                     DEEDEEEWEQEEEKSIN"
     gene            complement(398..1411)
                     /gene="fbp"
                     /locus_tag="VFJ67_01140"
     CDS             complement(398..1411)
                     /gene="fbp"
                     /locus_tag="VFJ67_01140"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004728647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HET7289013.1"
                     /translation="MIGVETLDEFIIRKQTELPYARGDLSRLLRDLGLAAKIVNREVN
                     KAGLVDILGSTGKMNVQGEEVKKLDVFANRRFIAALELGGQCSGVASEENKDFITFHD
                     EISKDASYVVALDPLDGSSNIDVNVSVGTIFSIYRRISPPDGPVTTEDFLQPGRHQVA
                     AGYIIYGSSTMLVYTTGYGVNGFTLDPSIGEFCLSHPDMKIPKAGKTFSVNQGNFYSF
                     PDGVKQFIKYCKEPDKATSRPYSLRYIGSMVADIHRNLITGGIFLYPATSDAPNGKLR
                     LLYECNPMSFVIEQAGGRSTDGSRHILDIEPRELHQRTPIFIGSEQLVLKLEEFLSAS
                     KKS"
     gene            complement(1517..>2077)
                     /locus_tag="VFJ67_01145"
     CDS             complement(1517..>2077)
                     /locus_tag="VFJ67_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET7289014.1"
                     /translation="ALAGFSKGIISQIFSIAALAGGLIIGFIFYDALGGVLVSENVVE
                     NRSIANVGSFIALVFVSYVIIQLAGWITTKLIGTLQLSWLNRICGGAAGAVMGAVTAF
                     LLASGLTLFYPENDPAFRNSALLPYLREGSLIVKDALPEDLEKSFNRAKELVREEGLE
                     AAMKIKDSDAVKEILRNTGDSKNKKN"
BASE COUNT          411 a          588 c          537 g          541 t
ORIGIN      
        1 tgaatatcag ttgatggact tttcttcttc ttgttcccac tcttcttcgt cttcctcgtc
       61 ccactcctcg tcttcatcca agccgtcttc gtcttcctcg tcccataact cttcttcgtc
      121 ctcgtcccac tcctcttctt catcgtcttc ccagtcatcg tccttagcaa aaagttcaag
      181 ttcgatttcc atgataattc tcctgtaacg gtgatttaaa tgaaacgata caccctgact
      241 ttgaaatgtc aagtaaattc cggggcgcga aatccgtccg atattatcag tattaaatga
      301 gcatgttaag ggattgtgaa cggttataaa cagtggctaa tccgtccctc gtgcggctct
      361 tttttccggg cggatttctt ccgagcactc ggcgcgctca gctcttttta ctcgcgctca
      421 ggaattcctc gagcttcaat acgagctgct cggagcctat gaaaatcgga gttctctgat
      481 gaagctccct gggctctatg tcgagtatat gtctcgatcc gtccgtcgat cttccgccgg
      541 cctgctcgat gacgaacgac atcgggttgc actcgtaaag cagtctcagt ttaccgttgg
      601 gagcgtccga ggtggcgggg tagaggaata ttcctccggt tatcagattc ctgtggatat
      661 ccgcgaccat cgagcctatg tacctgagcg agtaggggcg gctcgtcgct ttgtccggtt
      721 ctttgcagta tttgatgaat tgctttacgc catcgggaaa gctgtagaaa ttgccctggt
      781 tcaccgaaaa ggtctttccc gctttgggta ttttcatgtc agggtgcgag aggcagaatt
      841 cgccgatcga cgggtcgaga gtgaagccgt tcacgccgta tccggtcgta tagacgagca
      901 tggtggaaga gccgtaaata atatatccgg ccgctacctg atgtctgccc ggctggagga
      961 agtcctctgt agtaacgggc ccgtccggcg gcgatatcct cctgtagatc gagaatatgg
     1021 tcccgaccga aacgttcacg tctatgttcg acgagccgtc aagcgggtcg agagccacga
     1081 cgtaactcgc gtctttcgag atctcgtcgt gaaacgtgat gaagtcctta ttctcctccg
     1141 acgcgacgcc tgagcactgc cctccgagct cgagggcggc gatgaatctc cggttagcaa
     1201 agacgtcgag cttcttgacc tcttctccct ggacgttcat tttgcccgtg ctcccgagta
     1261 tgtcgacgag accggccttg tttacctccc tgttcactat cttggcagcg aggccgaggt
     1321 ccctcagcag cctggagagg tccccccgcg cgtagggcag ctcggtctgc ttcctgatga
     1381 tgaattcgtc tagtgtctca acgcctatca taacacgata atattaccga aattacgcca
     1441 ttaagaaagc ctgcgcccga atgcgccgaa cgcccggacg gggcgtgaga aattcgcctg
     1501 tatcgggggc cgctcgtcag ttttttttgt ttttcgagtc gccggtattt ctcagtatct
     1561 ccttcaccgc gtccgagtcc ttgatcttca tcgcggcttc gagcccctct tcccttacga
     1621 gctctttcgc ccggttgaag ctcttctcca ggtcctcggg gagcgcatct tttactatca
     1681 gagacccttc ccggagatat gggaggaggg cggagttcct gaaggcggga tcgttctccg
     1741 gatagaagag cgtgagtccg gacgcgagca gaaaagccgt gaccgcgccc atgacagcgc
     1801 ctgccgcgcc gccgcaaatc ctgttgagcc agctcagctg aagcgtgcct atgagtttcg
     1861 tcgttatcca gcccgccagc tgtattatca cgtacgagac gaatacgagc gcgatgaagc
     1921 tcccgacgtt ggctatcgac ctgttctcga cgacattttc gcttacgagc acgccgccca
     1981 aggcgtcgta gaatatgaac ccgattatga gccctccggc gagggcggct atagagaata
     2041 tctggctgat gatgcccttc gaaaaacctg cgagggc
//
LOCUS       DASSJH010000053         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1202085, whole genome shotgun sequence.
ACCESSION   DASSJH010000053 DASSJH010000000
VERSION     DASSJH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202085"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFJ67_01150"
     CDS             complement(<1..367)
                     /locus_tag="VFJ67_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289015.1"
                     /translation="MRQIGLRAEILVNILFITAVAMFLIGVIAFKVTERFAVQGKIES
                     VDTIIKAFEGSYLRNNDIEGGLEFLKGALTPGAWGVVSGNGERVYFSTAGGKDEGRKG
                     DPLVLESMRTGSRVTEVEGV"
     gene            complement(461..1294)
                     /locus_tag="VFJ67_01155"
     CDS             complement(461..1294)
                     /locus_tag="VFJ67_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET7289016.1"
                     /translation="MSEREALLYLFSFILGTVVGSFLNVCIYRLPKGESLAFPSSHCT
                     SCGKAIRFYNNIPILSYLILKGRCADCGSPIPLRYPAVELISGIVLAGLYYKFGLTLD
                     TLFYALLTYSLIVITFIDLNHMIIPNAITVPGAVAGLLYNALITDWARSRDLLGAFSF
                     SLENFFGVLNEVPFLDSVFGLIMGGGILFLIAFLYIAVRKQEGMGMGDVKLLAMIGAF
                     LGWKGVVFVMLVSSLLGTMTGLSIILYKKGDLKYALPFGPFLSLAAVIYIFTGGFRLI
                     N"
     gene            complement(1287..>2981)
                     /locus_tag="VFJ67_01160"
     CDS             complement(1287..>2981)
                     /locus_tag="VFJ67_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7289017.1"
                     /translation="RTSTKVDIKRKPKTKFDSSRVSFQDIGGLKEQISKVREIVELPL
                     KYPQLFDKLGIDPPRGILLVGPPGSGKTLLAKAVAHETNANFQVINGPEIMHKFYGES
                     EARLRDIFEIATRNQPSIIFLDELDAIAPKRDKVSGDVEKRIVSQLLSLMDGLKHRGN
                     IVVIGATNLPSAIDPALRRPGRFDREVTLEVPDRNGRAQILEVHTRGMPLGEDIDLDR
                     LADMTHGFVGADLENLCREAAMKSLRKTLPDYDDGEEELHLGNGNLTPIKVGMSDFME
                     ALKEVEPSAIREIFVEISEVTWDDIGGLKDIKDKLVEAVIWPLQHKDLFEITDTKAPK
                     GILLCGPPGTGKTLLAKALANQSGANFISVKGAELLSKYVGESEQAVRDVFKKAKQVA
                     PCIVFFDEIDALAPPRRESDGTRVSERVVSQLLTEMDGVEDLREVLVLAATNRMDIVD
                     TALLRAGRFDLIFNIPYPDEEELLEILKIHTRGKPMAKDAKLPEIAHKSRGLSGADIE
                     LLCQRASIIAIREHLRNRKKAIKITEGHFDEALAEIRSKIIANADIKKTHPEKESV"
BASE COUNT          587 a          866 c          784 g          744 t
ORIGIN      
        1 taaccccctc tacctcagtc acacggctac cagtcctcat ggattccagc acgagcgggt
       61 ctcctttccg gccttcgtcc tttccgccgg ccgtgctgaa ataaacacgc tcaccgtttc
      121 cggagaccac tccccatgcg ccaggggtga gcgcaccctt taaaaattct agtcctccct
      181 cgatgtcgtt gttcctgagg taggagcctt cgaacgcctt gattatcgta tcgacgctct
      241 cgatcttccc ctggacggcg aacctctccg tcaccttgaa cgctatcaca cctatgagga
      301 acatggcgac cgcggttata aagaggatgt ttacgagtat ttccgctcta agacctatct
      361 gtctcatact ggaggcgtta cttcccctgt ccccttaaaa tcggaggggc gcagttatcc
      421 cttttaccta agtttagtgc cattcaggcc gggctggtaa ttaatttatg agcctgaatc
      481 cgcctgtgaa gatgtagatt acggcggcga gtgagagaaa agggccgaag gggagggcgt
      541 atttgaggtc ccctttcttg tagaggataa tcgaaagacc ggtcattgtt cccaagagag
      601 agctcacgag catcacgaat acaacgcctt tccagccgag gaaggctcct atcatcgcaa
      661 ggagcttcac gtcccccata cccatgcctt cctgtttccg gacggcgatg taaaggaacg
      721 cgatgaggaa taatatgccc cctcccatga tcagcccgaa tacggaatcg aggaacggca
      781 cttcgttcag cacgccgaag aagttctcga gactgaagct gaacgcgccc aggaggtccc
      841 tgcttcttgc ccaatccgtg atgagcgcgt tatagaggag tcccgcaacc gcccccggta
      901 cggttatcgc gttcggtatt atcatgtggt ttaaatcgat gaaagtgatg acaatgagcg
      961 aataagtcaa cagagcataa aataaggtgt cgagcgtgag gccgaacttg taatacaaac
     1021 ccgcgagaac tatgcccgat atgagctcga ccgcgggata tcggagcggt atgggcgagc
     1081 cgcagtcggc gcaccttccc ttcaatatca gataactgag tatcgggatg ttgttgtaga
     1141 acctgattgc ttttccgcaa gatgtacagt gtgaggacgg aaacgcgagg gattccccct
     1201 tcggcagcct gtatatgcag acgttcagaa agctgcctac gaccgttccc agtatgaatg
     1261 aaaataaata gaggagggct tctctctcag acactctctt tttccgggtg cgtcttctta
     1321 atatccgcgt tcgctatgat cttgcttcta atctccgcaa gcgcttcgtc aaagtgccct
     1381 tcggtaatct ttatggcttt tttcctgttc cggaggtgct cccttatggc tattatggat
     1441 gccctctggc agaggagctc gatgtcggcg cctgaaagcc ctctcgactt atgtgcgatc
     1501 tcgggaagct tcgcgtcttt ggccatcggc tttcctcgtg tatgtatctt cagaatttcg
     1561 agcagctctt cctcgtccgg ataggggata ttgaatatga ggtcgaacct gccggcccgc
     1621 agcagcgccg tgtcgactat gtccatcctg ttcgtggccg caagcaccag cacctctctc
     1681 aggtcctcaa cgccgtccat ctccgtgagc agctggctga ccaccctctc cgatacccgg
     1741 gtcccgtccg attccctccg cgggggcgca agcgcgtcga tttcgtcgaa gaagactata
     1801 cacggcgcta cctgttttgc tttcttgaac acgtccctca ctgcctgctc ggattcgccc
     1861 acgtatttcg agagcagctc cgccccttta actgaaatga agttcgcccc gctctggttg
     1921 gcgagagcct tggcgaggag cgttttaccc gtgcccggag gcccgcagag aaggattccc
     1981 ttgggagcct tcgtgtccgt tatctcgaac aggtctttgt gctgaagagg ccatatcacg
     2041 gcctcaacga gcttgtcctt tatatccttg aggcccccta tgtcgtccca tgtcacttcc
     2101 gatatctcta cgaagatctc tctgattgcc gagggctcga cttccttgag cgcttccatg
     2161 aaatcgctca tgcccacttt tatcggcgtg aggttgccgt tcccgagatg cagctcttct
     2221 tcgccgtcgt cgtagtcagg aagggttttt ctcagcgatt tcatcgcagc ttcgcggcag
     2281 aggttctcca ggtcagcccc gacgaagccg tgcgtcatgt cggcgagacg gtcgaggtct
     2341 atgtcctccc cgagcggcat accgcgcgtg tggacttcga gtatctgggc gcgcccgttc
     2401 ctgtcgggca cttccagagt cacttcgcgg tcgaacctcc cgggcctcct cagggcgggg
     2461 tctatggcgg agggcaggtt ggtcgcgcct atcaccacga tgttgcccct gtgcttgagg
     2521 ccgtccatga gcgagaggag ctgggagacg attctcttct ccacgtcccc cgagaccttg
     2581 tccctcttgg gagcgatcgc gtcaagctca tcgaggaata ttatggacgg ctggttcctc
     2641 gtcgctatct cgaatatatc gcggagcctc gcctcgctct ccccgtaaaa tttgtgcatt
     2701 atctcggggc cgttgattac ctgaaagttg gcgtttgtct cgtgagcgac agccttggcg
     2761 agaagcgtct ttccgctgcc cggaggaccc acgaggagga tacccctcgg ggggtcgatg
     2821 ccgagcttgt cgaaaagctg cgggtatttg agtggaagct cgactatctc cctcaccttc
     2881 gatatctgct ccttcaatcc gccgatatcc tggaagctca cgcgggagct gtcgaacttc
     2941 gtcttcggct tcctttttat gtccaccttc gtcgaggttc t
//
LOCUS       DASSJH010000054         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_877321, whole genome shotgun sequence.
ACCESSION   DASSJH010000054 DASSJH010000000
VERSION     DASSJH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877321"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFJ67_01165"
     CDS             complement(<1..542)
                     /locus_tag="VFJ67_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET7289018.1"
                     /translation="MKPAVFALTVALMTAVVSLAADTQVPQRQKSQRSGYSLKSSAYF
                     MGRYSKNLSDMIDAALKMKLTAEQKSQVSAISGKYSDQMTRDESDVRKLRVNIPKMLN
                     DPSFDPAKVKAEIDKANALEKKISDDYVDALASLRDTIGKENYQTLTKALYKYRNDLV
                     QMRKDRQTLNRQPGLIKSEPA"
     gene            complement(657..1217)
                     /locus_tag="VFJ67_01170"
     CDS             complement(657..1217)
                     /locus_tag="VFJ67_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289019.1"
                     /translation="MLVNWDNLGISGESNFLQLFYDPHSETLVAHFFRSIGGGYGLRS
                     LWTRRVTDERYVRITSSDSYLSYEDIVLCPSSAHVFANVMRVEKKGASFDGYEWHSIQ
                     RIDLLTGEAEVIAESGSEGFDTGQEKIWISTLHGASADCRELYCSAAVQKKGKGAVSP
                     TEYYLSRLVIGEGRIERITRLSATFL"
     gene            complement(1636..>2319)
                     /locus_tag="VFJ67_01175"
     CDS             complement(1636..>2319)
                     /locus_tag="VFJ67_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7289020.1"
                     /translation="GQAEYVRVPFADTTLEKVPGGLADERVLFLGDIFSTAYFCAEWG
                     KIQPGNTVVVFGDGPLGLLATASAKLFGPSRIITVGHHDFRLDIAKKMGADVVINSKN
                     DNVVERVMELTNGQGADVCLECIGSTHAVLDAIKIARPGGTISFIGFMFQELPIPMLD
                     FYLKDLTFRGGVCPAKNYIRKLLPLIETGKIDPTAVITHDLPLSDTPRGYELMDSKIE
                     NAIKVVLTP"
BASE COUNT          482 a          621 c          630 g          586 t
ORIGIN      
        1 gcaggctcgc tcttgattag gcccggctgc cggttgagcg tttgcctgtc cttcctcatc
       61 tgaactaggt cgttacggta tttatagagg gcctttgtca atgtctggta attctccttg
      121 cctatcgtgt ctctgagaga cgcgagagcg tccacgtaat catcggaaat cttcttctcg
      181 agcgcgttcg ctttgtcgat ctccgctttc accttggccg gatcgaatga agggtcattc
      241 agcatcttcg gtatatttac cctcagcttt ctcacgtcgg actcgtccct ggtcatctgg
      301 tccgagtatt tcccggagat agcggagact tgagactttt gttccgcggt caatttcatt
      361 ttaagcgccg cgtctatcat atcgctgagg ttcttcgagt accttcccat gaaataggcg
      421 cttgacttca gggagtagcc gctcctctga ctcttctgcc gctggggtac ctgagtgtcg
      481 gctgcgaggg aaactactgc cgtcatcagg gcgacagtta aagcaaatac tgcgggtttc
      541 attaatacta cctccgttcg gttatatggc gatatagtgt aaccaatatt gcggcggttt
      601 tcgtcaggaa atttcagggg gaatacgacg catgccggtt gaagtatgag ccgatcctag
      661 agaaacgttg cagagagcct ggttatgcgc tcgatcctgc cttctccaat gacgagcctc
      721 gacagatagt actcggtcgg ggagaccgct ccttttccct ttttctgaac cgcggccgag
      781 cagtagagct ccctgcagtc ggcgctcgcc ccgtggagcg tgcttatcca tatcttctcc
      841 tgcccggtgt cgaagccctc gctcccgctc tccgcgatga cttccgcctc tcccgtaaga
      901 aggtcgatgc gctgaatgga atgccactcg tacccgtcga agctcgcccc ctttttctcg
      961 acgcgcatca cattggcgaa tacgtgagcc gaagagggac agagcacgat gtcctcgtag
     1021 ctgaggtagc tgtcggaaga ggttatcctg acgtacctct cgtccgtcac gcggcgggtc
     1081 cagaggctcc tcaacccgta gccgccccct atgcttctga agaaatgcgc gacgagcgtt
     1141 tcggaatggg gatcgtaaaa gagctgaaga aagttgcttt cccctgatat gccgagattg
     1201 tcccaattaa cgagcatacg tcatcctctt tggtatccat cggattgtat ttatatgttc
     1261 cggtcacggc acggagcttt ctgatacatg cgtatgcgca gaaagacata atattattaa
     1321 catttttatg gcctttgtaa agttttaatt aaggagggtc tgttcagggg tatcgtttag
     1381 ggaagttccg aatgagctgt cagcgagtcg tcgcagacaa ataattatgc aacccgttca
     1441 tacttcgaca agctcagtac gaacgggttg ggtttatttg caggtatgag ctaaagactt
     1501 acagaaggat caaatacgag cgcagattct gcggctgata gcatccgcag gcaaacgaac
     1561 gttaataatg tccgcgttac tttcccctca tccgagcaca tggaaggaat cattcatcag
     1621 cggacattgt ccgcgtcagg gggtgaggac gacctttatc gcgttctcga tcttcgaatc
     1681 catgagctcg tatcccctgg gcgtgtccga gagcgggagg tcgtgagtta tgaccgccgt
     1741 cgggtcgatc ttgcccgtct cgatcagggg caggagcttc cttatatagt tcttcgccgg
     1801 gcagacgccc cctctgaacg tgaggtcttt caaatagaaa tcgagcatgg gtatcgggag
     1861 ctcctggaac atgaacccga tgaacgatat ggtgccgccg ggcctggcga tcttgatggc
     1921 gtcgagcacg gcgtgagtcg agccgatgca ttcgaggcag acgtcggcgc cctggccgtt
     1981 tgtgagctcc ataaccctct cgacaacgtt atcgtttttt gagttgatta cgacatccgc
     2041 tcccatcttc ttggctatat cgagcctgaa gtcgtggtgc ccgacagtga taatgcggga
     2101 agggccgaat aatttggcgg aagccgtcgc gaggaggccg agcggcccgt cgccgaatac
     2161 gacgaccgtg ttgccgggct gtatcttgcc ccactcggcg cagaaatagg cggtcgagaa
     2221 tatatccccg agaaagagca ccctctcgtc ggcaagtccg cccgggacct tctcgagagt
     2281 cgtgtcggcg aaaggcaccc taacgtactc cgcctgccc
//
LOCUS       DASSJH010000055         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1311353, whole genome shotgun sequence.
ACCESSION   DASSJH010000055 DASSJH010000000
VERSION     DASSJH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311353"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFJ67_01180"
     CDS             complement(<1..743)
                     /locus_tag="VFJ67_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289021.1"
                     /translation="MARKMIIHHPDISSKDGKLTVSARIEFKTPLPGMPERLWFEFPE
                     DYNDLVTDRADAFLPGMVLVAMQYGEDIEVRGEISPRLLIGINEYQMPFCMWFPRRFK
                     PIRIECESPIPPGTENGRDIVMSAFSGGVDSFFTLKSYLPGNELPKSVQISHLLFVHG
                     FDIKLEYPKSFPLLRDAYSDMCDRLGIKLISCGTNCQQFGTRRNWGLFHGAALIGAAH
                     AVGRRTAMFHVPSSHSFTSLMPWGSDPGI"
     gene            complement(728..2236)
                     /locus_tag="VFJ67_01185"
     CDS             complement(728..2236)
                     /locus_tag="VFJ67_01185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7289022.1"
                     /translation="MKKATFILTVPNASAGLNETLKSIFVQRGEEDQTICIVESSAYS
                     SLRESLARDYTTALLIESDFKSEHTGSILNRGIALSEGSTIFLITQPVILLPGFVTSY
                     IEYLYSKESIGYVYGNYIEERAQGEEAGIAKTDNFDYSEASVIGPVYAMRREVLDAVG
                     YYDTGLKYSFEYDLRLRIFERFEIARVEESLYRVLYTTGNPGLTGFANPDRSYLDYSQ
                     EEEGEYRDSCYASLKRRGAFLSGPPVRATEGTSKDPAGPEISVVIPLYNREKYIGRTI
                     ESVLKSEWKRYEIIIVDNGSTDNGFEAASKYTGRGDITVLRSEIACNTAAALNTGIRH
                     ARGKYICQLDSDDLYTSDALSIMYDYMESNPGFALGVSYYDCIGTDDNVLEEYRVVKH
                     EEYDRNNILRTDGVGAARIWRRTVLEEMNGFDEINLGSYAEDYDIVLRVTEKYDILRI
                     PYVLYHYRINHKEIGEEADYILRHEKKTFARRSAIKRRQMINGSLSRVWLVK"
BASE COUNT          528 a          620 c          582 g          655 t
ORIGIN      
        1 tcgatgcccg ggtcggagcc ccagggcatg agacttgtaa agctgtgaga cgatgggacg
       61 tggaacatag cggtccgtcg tcctactgcg tgcgcggcgc cgatcagggc cgcgccgtgg
      121 aataaacccc agttccgccg cgtgccgaat tgctggcagt tcgttccgca gcttatcagc
      181 tttatcccga gtctgtcgca catatccgag taggcgtctc tcagaagcgg aaaggattta
      241 gggtattcca gctttatgtc gaagccgtgt acgaatagaa ggtgcgatat ctgtacggat
      301 ttcggaagct cgtttcccgg cagataagac tttaaggtga aaaaggaatc cactccgccg
      361 gagaacgccg acatcacaat atcccggccg ttttcagtgc ccggggggat cggggattcg
      421 cactctatac ggatcggttt gaatctccgg ggaaaccaca tgcagaaggg catttgatat
      481 tcgttgatcc cgataagaag acgcggggag atttctcctc tcacctctat atcctcgccg
      541 tactgcatgg ccacgagcac cattccgggg aggaaggcgt cggccctgtc tgtaacaaga
      601 tcgttgtagt cttcgggaaa ctcgaaccag agcctctcgg gcatgccggg gagaggggtt
      661 ttgaactcga tcctggccga tactgtaagc ttcccgtctt tagaagatat atccggatga
      721 tggattatca ttttacgagc cacactctcg aaagcgagcc gttaatcatc tgccttcttt
      781 taatagccga tcttctcgca aaggttttct tttcgtgtct caatatataa tcggcttcct
      841 ctccgatttc cttgtggtta atcctgtaat ggtaaagcac ataaggtatg cgtaaaatgt
      901 cgtatttttc ggtcactctc aggacgatgt cgtaatcctc ggcgtagctt ccgaggttga
      961 tttcgtcaaa accgttcatc tcttcgagga cggttcgtct ccatatgcgg gctgcgccga
     1021 ctccatcggt cctgaggatg ttattcctgt cgtattcctc gtgtttcaca acacggtatt
     1081 cctccaacac attgtcgtcg gtccctatgc agtcgtaata ggaaacgccg agtgcgaatc
     1141 cggggttgga ttccatatag tcatacatta tagaaagcgc atcggaggtg taaaggtcgt
     1201 ccgagtcgag ctgacaaata tactttcccc ttgcgtgcct tatccccgta tttagggcgg
     1261 cggcagtgtt gcacgcgatt tcgctcctga ggacggttat gtcgcccctc ccggtgtact
     1321 tcgatgcggc ttcgaagccg ttgtccgtgg agccgttgtc cacgattatg atctcgtatc
     1381 ttttccactc actctttaat acgctttcga tagtacgtcc aatatatttt tcacggttgt
     1441 agagaggaat gacgacgctt atctccggcc cggccgggtc tttcgatgtg ccttccgtcg
     1501 ctcggacggg cgggccggag agaaacgccc cgcgcctttt aagcgaggcg tagcaggaat
     1561 ccctgtactc gccttcttcc tcctggctgt aatcgagata tgaacggtcc ggatttgcaa
     1621 aacctgttaa tcccggattc ccggtggtat ataaaaccct gtaaagagac tcctcgacgc
     1681 gggctatctc gaatctttcg aaaatcctta acctgaggtc atattcgaaa gagtatttca
     1741 atccggtgtc gtaataaccg accgcatcga gcacctccct cctcatggcg tatacgggcc
     1801 caatcacgga tgcttccgag tagtcgaaat tatcggtttt tgctatcccg gcttcctccc
     1861 cctgtgctct ttcctcgatg taattaccat atacgtaacc tattgattcc ttagaataca
     1921 gatattcgat atagctggtt acgaagcccg gaaggagtat caccggctgg gttatcagga
     1981 aaatcgtact tccttcagaa agagcgattc ccctgttgag gatgctgcct gtatgctccg
     2041 atttgaaatc cgattcaatt aacaacgccg tggtgtaatc tcgagctagg gattctctca
     2101 aagagctata tgccgagctt tctacgatgc atattgtctg atcctcctct ccacgctgaa
     2161 cgaaaatgct ttttagcgtt tcattcaatc cggcggatgc gtttgggaca gtcagtatga
     2221 aagtcgcttt cttcattgtc ggtacacagc ccgaatgtgt tgacctaatt ctcacgatct
     2281 gaagacagga acaccagcaa gttattcctg agcatctccg ataatgctct caaatcgagg
     2341 tatctatcat atacgctctt ctcttccatc ttcatcgtat tcttc
//
LOCUS       DASSJH010000056         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_877417, whole genome shotgun sequence.
ACCESSION   DASSJH010000056 DASSJH010000000
VERSION     DASSJH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877417"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFJ67_01190"
     CDS             complement(<1..794)
                     /locus_tag="VFJ67_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7289023.1"
                     /translation="MRINIGLYTSLILALLFVSVTAAAVKKEAEVIFEAEREKEPIPL
                     LTENYPGLTLERAYEIQSEYVILKLGGDTIAGYKAGLTSKGAQAKFGVSTPVSGVLFS
                     SGKHVSPASIDGSKYRSPVIETEIGFVVGKPIREKIADTGQLLEHIGAVLPVVEIPET
                     GFADMEKIKAEDIIATNVGSSIFIAGVEEPLGAVDPDDITVALVSGDKTLNRGVGPDA
                     MGDQLQALRWLVNSVVEHGRTIDTGSILITGSLGKVVPASLGRYEAR"
     gene            complement(796..1761)
                     /locus_tag="VFJ67_01195"
     CDS             complement(796..1761)
                     /locus_tag="VFJ67_01195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7289024.1"
                     /translation="MPDKNAVILGAGVSGLTCGVRLLEAGFSVRIKAHTLPPHTTSNV
                     APAYWYPYKVAPRERVLGWADFSYAKLTALSLVPGSGVSMSSLLKLFEEPVDEPFWKP
                     AVRTFRRARPEELPPGYADGFVTEVPRIETPVYMRYLEDSFVELGGELEKLEREAASI
                     EELMGDRLILINCAGLGAGKLCRDENTFPIRGQIVIVKNIGLERITSVDTGRQAPIYI
                     VPRDGDCILGGTAEENNWDLRVNPETSKKIIEKCAAIEPALVNAEILGHRVGLRPGRT
                     GVRLELEELPGGRAVIHDYGHGGAGFTLSWGCAEEVLNLAEGFLF"
     gene            complement(1766..>2536)
                     /locus_tag="VFJ67_01200"
     CDS             complement(1766..>2536)
                     /locus_tag="VFJ67_01200"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HET7289025.1"
                     /translation="GWTSSSETGEHAPEPSYAERVRTLVYRNTIGTLSTNSKKHPGSP
                     FGSLMPYGLDGKGRPIFLISTMAVHTQNLEHDPHASLFISQTDSQGDPLDASRVTLMG
                     EVSKVPEGETPEARELYLSRYKNASYWVDFDDFSFYRMEVRDIYFVGGFGAMGWVTAE
                     DYSGAQVDPLADVSKWIITHMNEDHRDSLIALARKFTGVEAEDASMTSVDRLGFNVRL
                     RAGSEVFSRRIGFTREARSKEEAREVIVEMVKLAGEGV"
BASE COUNT          511 a          814 c          635 g          576 t
ORIGIN      
        1 cgcgcctcgt aacgtcccag tgaggcgggc acgaccttac cgagtgagcc ggtaatcagg
       61 atactcccgg tatctatcgt ccggccatgc tcgacaaccg aattcacgag ccagcggaga
      121 gcctgcagct gatctcccat cgcgtccggt cccacgcccc ggtttagggt cttatcgccc
      181 gagaccagcg cgacagttat atcatccggg tcgacggcgc cgagcggctc ctcaacaccg
      241 gcgatgaaaa ttgatgaccc aacgttcgta gcgatgatgt cctcagcctt tattttctcc
      301 atatccgcga atcccgtctc gggaatctcg acaacaggca ataccgcgcc tatatgctcc
      361 agaagctgtc ccgtatccgc gatcttttcc ctgataggtt tcccgaccac aaaacctatc
      421 tcggtctcga tcaccggact ccggtatttc gagccgtcga tagaagcagg gctcacgtgc
      481 ttgcccgacg agaacagtac gcccgatact ggagtgctca cgccgaattt tgcctgagcc
      541 cctttcgagg taagccccgc tttatacccg gctatcgtat cgcctccgag ctttagaatg
      601 acgtattcgg attgaatctc ataagcccgc tcaagcgtca ggcccggata attctcagtc
      661 agaagaggta tgggctcttt ctctcgctcg gcctcgaata tgacttcggc ttcttttttc
      721 acggccgctg cggttacaga cacgaaaagg agcgcgagta tgagcgatgt gtatagtccg
      781 atattgattc tcatttcaga aaaggaaacc ctccgccaga ttgagcactt cctctgcgca
      841 gccccaggat aaggtaaacc ctgcccctcc gtgaccgtag tcgtgtatca cagcccggcc
      901 tccgggcagc tcttcgagct cgagccttac cccggtcctc cccggcctga gcccgaccct
      961 gtgccccagg atttccgcgt ttaccagcgc gggctcgatc gccgcgcatt tctctatgat
     1021 tttttttgat gtttccgggt tgactctcag atcccagtta ttctcctcgg ctgtccctcc
     1081 caatatacag tccccgtccc tcggcactat atagattggg gcctgcctgc ccgtatcgac
     1141 gctggtaata cgctcaagcc cgatattttt cacgatcact atctgcccgc ggatcgggaa
     1201 cgtattttca tcacggcata atttccccgc cccgagccct gcgcagttaa tcaggatcag
     1261 ccggtcaccc attagttctt cgatagaagc cgcttccctt tcgagctttt ccaattctcc
     1321 gccaagctcg acaaaactat cctcgagata acgcatatat acaggtgtct ctatgcgcgg
     1381 cacctcggtg acgaagccgt ccgcgtaacc gggcgggagc tcttcaggcc ttgctctcct
     1441 gaacgtcctc accgccggct tccagaaggg ctcgtcaacg ggctcttcga atagcttgag
     1501 gagactcgac atggatacgc cgctgccggg tacaagactg agagcggtaa gcttggcata
     1561 ggagaagtcc gcccatccga gcaccctttc ccttggagcg actttatagg gataccagta
     1621 tgcgggagcg acgttcgatg tcgtgtgtgg aggaagcgtg tgagctttaa tcctcacgct
     1681 gaaacccgcc tccaaaagtc tcaccccgca cgtaaggccc gacacgccag caccgagaat
     1741 gacggcgttt ttatccggca tggacctata ctccctctcc ggccagcttc accatctcca
     1801 ctattacctc ccgcgcctct tccttcgagc gcgcctccct cgtaaatccg atccgcctgc
     1861 tgaacacctc cgagcccgcc ctgagacgca cgttaaaccc gagcctgtcg acggacgtca
     1921 tcgaagcgtc ctcagcctct acccccgtaa acttacgggc gagcgcaatt agcgagtcgc
     1981 ggtggtcttc gttcatgtgc gtaattatcc acttggaaac gtctgcgagc gggtctactt
     2041 gagcgcctga gtaatcctca gccgtcaccc agcccatcgc gccgaaccct ccaacgaaat
     2101 atatgtccct gacctccatg cggtagaatg agaagtcgtc gaaatcgacc cagtaactcg
     2161 cgtttttgta acgcgagaga tagagctccc tcgcctcggg agtctcgcct tcgggaacct
     2221 tcgacacctc gcccatgagc gtgacgcggg atgcgtcgag cgggtctccc tgtgagtcgg
     2281 tctgagatat aaacaggctc gcgtgcgggt cgtgctcgag gttctgcgta tgcacagcca
     2341 tcgtgcttat gaggaatatc ggcctgccct tcccgtcgag cccgtacggc atcagagagc
     2401 cgaacggcga gcccgggtgc ttcttcgaat ttgtagaaag cgtcccgatc gtgttcctgt
     2461 aaacgagagt cctcacacgt tccgcgtatg aaggctcggg cgcatgctcc ccggtttccg
     2521 aagatgacgt ccatcc
//
LOCUS       DASSJH010000057         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_663391, whole genome shotgun sequence.
ACCESSION   DASSJH010000057 DASSJH010000000
VERSION     DASSJH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663391"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..584
                     /locus_tag="VFJ67_01205"
     CDS             48..584
                     /locus_tag="VFJ67_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7289026.1"
                     /translation="MSKELTQEQQKMVDVWGKHTMCEFETKDVDETMRTMTGEPHVNH
                     VPVMTGGCGREDVRDFYSKYFIPFQSPNTELALVSRTVSHDTIVDELIFKFTHNVYMP
                     WMLPGIPPTGKKVELPLVVIVGFRDGKIASEHIYWDQATVLVQIGVLDPHNLPVAGVE
                     TARKVLNPALPSNQLMME"
     gene            complement(587..973)
                     /gene="folK"
                     /locus_tag="VFJ67_01210"
     CDS             complement(587..973)
                     /gene="folK"
                     /locus_tag="VFJ67_01210"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7289027.1"
                     /translation="MNRAVIGLGSNIDPEENIGKARGAIASEFNIIRSSSFVETEPIG
                     YKEQRNFINGALLIETDMDVQGLKSWLRTLESDLGRVRSENRDGPRTIDLDILVWNGE
                     VVDGEVYERNFLKTFIGELLPGLDLD"
     gene            complement(998..1345)
                     /gene="folB"
                     /locus_tag="VFJ67_01215"
     CDS             complement(998..1345)
                     /gene="folB"
                     /locus_tag="VFJ67_01215"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014976083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET7289028.1"
                     /translation="MIIKIENLRLRTVVGIFEWEKKIKQDIVINIEIEFDGREAVEKD
                     DISFTVDYKSVTKRIISEIEGREFNLIERIAGDVVKIIIEDGRVGRATVKVDKPGALR
                     FADSVSVIHSESR"
     gene            complement(1361..2071)
                     /locus_tag="VFJ67_01220"
     CDS             complement(1361..2071)
                     /locus_tag="VFJ67_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7289029.1"
                     /translation="MKKAALITGAGDRLGKAMALALAGMGYDIAVHYNSSGKGAERTA
                     EEIRSLGRKCETFKADLSHMKSIKALTSKVFRSFPYCSLLINNSSIFEDIDFMDVTEE
                     SFDKEFNLNFKAPFFLSREFSRRKSAEVIVNMLDARVSRIESGHFVYNLSKRALRDFT
                     LMAAKALGPKIRVNGICPGPILPPPGRDTKYMTRISKSTPLRKAGSPDYVITALKYIL
                     DNDYVTGECLFVDGGQHI"
     gene            2340..3110
                     /locus_tag="VFJ67_01225"
     CDS             2340..3110
                     /locus_tag="VFJ67_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7289030.1"
                     /translation="MAKKPVKSKTPVLGIDIGGTGIKSAPVDIEKGVLLEERYRVDTP
                     HPATPRSMLAAIGGIISHWNWKGPLGCGFPGVMKSGEVYTAANLSKKWIGVNIRDRIE
                     KLSPCKAVVINDADSAGLAEMKFGAGKEYNRHGGGVVLMVTLGTGVGTALFVDSHLVR
                     NTELGHIEIDGEDAEKRTAEIHREIEGLSWKKWGKRVNKYLLHIEKLLSPDLIIIGGG
                     ISKKADKFFPYIQIRAHIVPAKMLNDAGIVGAALASEL"
BASE COUNT          748 a          844 c          858 g          829 t
ORIGIN      
        1 accggagacc gcataaagtt cgaatatagt atttagggag gtattcgatg agtaaggagc
       61 ttacgcagga acagcagaaa atggtggatg tttggggaaa acacaccatg tgtgaattcg
      121 agaccaaaga tgtggatgaa acgatgcgca ctatgacggg cgagccgcac gtcaatcacg
      181 tgcctgtaat gaccggaggc tgcggcaggg aggatgtacg cgatttttat tcgaaatact
      241 ttattccgtt ccaatcccct aataccgagc tcgcgttggt ttcgcgtacg gtcagccacg
      301 acacaatcgt cgatgagctt atcttcaagt ttacacacaa cgtgtacatg ccctggatgc
      361 ttcccggcat acctcccacc gggaaaaagg tggagcttcc gctcgtcgtg atcgtcgggt
      421 ttagggacgg caagattgcg agcgagcata tttactggga tcaggcaacg gtactggttc
      481 agattggagt cctcgatccg cataatctcc ccgtagcggg ggtggaaacg gcgcgtaagg
      541 tcctcaaccc ggctttgcct tctaatcaat tgatgatgga ataaagtcag tctagatcga
      601 ggccgggaag aagctccccg ataaacgttt tcaggaaatt cctctcataa acttctccat
      661 ccactacctc gccgttccag acgagtatgt cgaggtcgat tgtcctggga ccgtccctgt
      721 tctccgacct cacccttccc aggtccgatt cgagcgtcct cagccacgat ttcaggcctt
      781 gtacgtccat gtccgtctca atcagcagcg caccgtttat aaagttccgc tgctccttgt
      841 aaccaatggg ctctgtctcg acgaacgaag aggatcttat aatattgaat tccgaggcga
      901 tcgctccccg tgccttccct atattttctt cgggatcgat attggagccg agccctatca
      961 ccgccctgtt catggtcgta ggttgaatgt tcgaaggtca cctcgattcc gaatgaatta
     1021 cggaaaccga atccgcaaag cgcagcgcgc ccggcttgtc gaccttgacg gtcgccctcc
     1081 caactctccc gtcttcgatg attattttca cgacgtctcc tgcgatcctc tcgatcagat
     1141 tgaactccct gccttctatt tctgaaatga ttcttttcgt gacggattta taatcgaccg
     1201 tgaacgatat atcgtccttt tccacggcct ctctgccgtc gaattcgatc tctatgttga
     1261 tgacgatgtc ctgctttatc tttttctccc attcgaagat accgaccacc gtcctcaatc
     1321 taagattttc tatctttatt atcactctat actccaatca tcagatgtgc tgtccgccgt
     1381 cgacgaagag acactctccg gtgacatagt cgttatcgag tatatatttt aatgccgtta
     1441 tcacgtagtc ggggctgccg gccttccgga gcggcgtgct tttcgatatg cgcgtcatat
     1501 acttggtgtc cctgcccgga ggggggagta tgggccccgg gcatattccg ttgacccgta
     1561 ttttaggccc gagcgctttt gcggccatga gcgtgaagtc gcggagcgct ctcttgctga
     1621 ggttgtaaac gaagtggccg ctctcgatcc gggagaccct cgcgtcgagc atgttgacta
     1681 ttacctccgc cgacttccgc ctactgaatt ccctcgagag aaagaaaggc gccttgaaat
     1741 ttagattgaa ttccttgtcg aacgattctt ccgtaacgtc catgaaatct atgtcttcga
     1801 atatcgagga gttgttgatg agaagggagc agtaaggaaa cgaacggaat actttcgatg
     1861 tcaatgcctt tatgctcttc atgtgagaga ggtcggcttt gaatgtctcg cactttcttc
     1921 cgagtgatct tatctcctcg gccgtcctct cagccccctt cccggagctg ttgtagtgaa
     1981 cggctatgtc gtagcccatc cccgcaaggg cgagcgccat cgccttgccg agcctatccc
     2041 ccgctcctgt aattaaagcg gcttttttca ttacctatta ctctccggct tccctttgta
     2101 ctggttttta aacaccgaga gtatagcaca acgggcgctc gcggctaaga cgtacaggcc
     2161 ttggatcggt atcatcaatc acgatcccga gttttgttta cggatactgt ctctgtcctg
     2221 agcgcaaggg gacttcttcg gttttagcgg tactctgaaa ttgtcaatcg ggtttcatat
     2281 cccgcgccga attggtgtag aattaacacg gttttattca acatctcaga ggcggttata
     2341 tggcaaaaaa accggttaaa tcaaaaaccc cggtcctcgg tatagacatc ggagggaccg
     2401 ggatcaaatc ggcgccagtc gatatcgaga agggtgtttt acttgaggaa agataccgcg
     2461 tcgatacccc tcacccggcg acacccagga gcatgctcgc cgctatcggc ggaattatct
     2521 cgcactggaa ctggaaaggg ccgctcggct gcggattccc gggagtgatg aagagcgggg
     2581 aggtgtacac ggccgcgaac ctttccaaga aatggatagg agtcaatatc agggaccgga
     2641 tcgagaagct ttctccctgc aaggcggttg tgataaacga cgccgactcg gccgggctcg
     2701 ccgaaatgaa gttcggcgcc gggaaggaat ataaccgtca cgggggcggt gtggtcctga
     2761 tggtcacact cgggacaggc gtcgggacgg cgctattcgt cgacagccat ctcgtcagga
     2821 acaccgaact cgggcacata gagatagacg gcgaagacgc ggaaaagcgc actgctgaga
     2881 tccacaggga gatagagggc ctcagctgga agaaatgggg aaagagggtg aacaaatacc
     2941 tcctgcacat cgagaagctg ctctctcccg atctgattat aatcgggggt ggaataagca
     3001 agaaggcgga taaatttttc ccgtatatac agataagggc gcacatagtg ccggccaaga
     3061 tgctgaacga cgccggcata gtaggggcgg ctctggcttc ggagctttga tgacggggct
     3121 ttccttgaat tctgaggttc gttgtgagat aatggcaggg gtcgcgttct ttttatcctg
     3181 aattgatgac tctgcttttc cgaggtcgca atttgtttca cggggaatct cgcaggcatt
     3241 taattaaagg ggtttcagag gctgtccgtc aaattcaac
//
LOCUS       DASSJH010000058         7270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1312353, whole genome shotgun sequence.
ACCESSION   DASSJH010000058 DASSJH010000000
VERSION     DASSJH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312353"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFJ67_01230"
     CDS             <1..855
                     /locus_tag="VFJ67_01230"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7289031.1"
                     /translation="HVGYYREMSRTNVRIETCPVADTVINKAISRLSKVLASVGDPRY
                     PLDKIYLSSDGEKAHITLIPRFKSGPASLGSLAKHLRRHEETEDVSVHGEDEAGFEIS
                     VAGLRFIVTPSVFTQSNTAVNEALIRTVIEWAGLKGTETVLDLYSGAGNFSIPLAKSS
                     KQVISVEVSKKAVELAKKSAGLNGANNIGFHNVSCGEYLNSTDALAGSVDIVVLDPPR
                     EGAKEAVAGIKRLEAGKIIYVSCDPATLARDIKLFKEAGYRLERLKPFDMFPQTYHIE
                     SLSLLVRA"
     gene            complement(886..1557)
                     /locus_tag="VFJ67_01235"
     CDS             complement(886..1557)
                     /locus_tag="VFJ67_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7289032.1"
                     /translation="MNKALGIRVSLQSRGNGIGSGKSVLILSNHLSYVDIFVISSFFP
                     VSFIASVDEVKSDLLLGKATELSGGYFVERRSRSGLRTEIESISEILELGINVALFPE
                     GTTSNGDRVLPFKTPLLSIAQKAGVEILPLCLKYTSIDGEALTDKNKDLIYYYGDMKF
                     FDHLFKLLSVGYVEAELKVLDKIDPSTASSRKEMARSVYDMINSAYMNEGSAEMDAKN
                     RQAVF"
     gene            complement(1696..2556)
                     /locus_tag="VFJ67_01240"
     CDS             complement(1696..2556)
                     /locus_tag="VFJ67_01240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HET7289033.1"
                     /translation="MLPKKVISLKDTVTTSNVLKKAFRWKLRQFKPKVHLYIESSRFL
                     IKTVENSSELEKALNLRYEVFYRETLNKENFSGTDIDKFDQFCDHLIILDKKQNIVVG
                     TYRFISSTFSDKFYSETEFSMDQIKSAPGIKLELGRACVHKDYRNGTGIALLWKGLSE
                     YFKKINAKYLFGCSSIGTTNIVEISLIYKYIKELYLSPENFRVYPNEKHRIKELDYYL
                     EAFEKFGIRTESIEDFIPPLLKSYLKAGSVICGEPAIDKKFKCADFFTVLDLDLITKS
                     FEKKYKAPND"
     gene            3034..4089
                     /locus_tag="VFJ67_01245"
     CDS             3034..4089
                     /locus_tag="VFJ67_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HET7289034.1"
                     /translation="MKVLFGIQGTGNGHISRCRELLKHLLRRAEVDVLVSGYHHEVDL
                     GFEVKYKLPGLGFTFGKRGGIDYWNSVRNFSPYKLISDIYKIPVHRYDLVISDFEPIT
                     AWSAKLRSAPSVSLSHQAAFLSPNIPRPRQENRIQELIIKWYSPSPSHIGLHFQEYDE
                     FIFTPIIREEIRNTDVRNRGHYTVYLPSYDYSFLSGLFRRIDVKWEVFSKHHKGEPYR
                     DSNVTVYPVNNGEFVRSLSECEGILCNAGFETPAETLYLGKKLMVVPMRRQYEQQCNA
                     EALERLGVPVVRDLGNEFENKLCDWINSSFAFNADYKNNLPLIVERIFDSSSRADALN
                     DYIQDPGLENEGAGAAD"
     gene            complement(4142..4726)
                     /locus_tag="VFJ67_01250"
     CDS             complement(4142..4726)
                     /locus_tag="VFJ67_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289035.1"
                     /translation="METDNLDRSLLESRFERLISKSKNYINSRNEGELESLRNDLIHL
                     KLKIETEIEKSRKKHNPNNVTVLRLMLSIIQKFMCAVHLMENLKSKKPMCTDFDTKQM
                     LDILLPHIRILVENENWREKLDTKKLEEDYSLIESVEDQHKRIMLTYLIQDWHNIDEV
                     LNLLKIGELYKNIAEKLVNLSFITNNGDKKLKVS"
     gene            complement(5102..6088)
                     /locus_tag="VFJ67_01255"
     CDS             complement(5102..6088)
                     /locus_tag="VFJ67_01255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET7289036.1"
                     /translation="MKIMLVTDAWHPQVNGVVHTWSYITNLIRKDHEVTVLNPYIEGT
                     ELLPLKLQGNIPILKNANQIVNRYYDEHLPDRVHIATEGSLGLAMRQFCRKNKINYNT
                     SYHTRLADYGWVLYGVPTFLTWLYVRWFHRCSRKVLVTTKSIAGQLGLKNCIVWGRGV
                     DTRLFNPGDGEKTSGEKTIITVARVSKDKNLDDFCRVPGYRKILVGDGPYLNTLKARY
                     PDVEFAGYVPHNELKDIYTQAQVFVFPSMFDTFGLVILEAMACGLPVVAYDVPSPNDI
                     IQDGVTGYLGESLEENIEKAFRNLDGLSRNALDYARSQSWKSIADQFIDHLM"
     gene            complement(6137..6775)
                     /locus_tag="VFJ67_01260"
     CDS             complement(6137..6775)
                     /locus_tag="VFJ67_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7289037.1"
                     /translation="METEKKYYRETTEPILIPLDWRDRSARLISNLFSPPLIALACIV
                     VTALATRSESSLFWILVFIALLIIPPTIFVLTLVHRGKVSDFHMNIREERARPLFAIF
                     AYAAIIFLVMYLGGAPKLMLIVTAAALLQILLVFVITLKWKISGHCTSAAGLAFLTVA
                     LYGENVLPLTLIVPIIAWSRIRLGRHTISQTLAGSFLGAATVIIILYLANII"
     gene            complement(6765..>7270)
                     /locus_tag="VFJ67_01265"
     CDS             complement(6765..>7270)
                     /locus_tag="VFJ67_01265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7289038.1"
                     /translation="TQWLLFTDADIVFSRDYFINVGNLAECDLIYGPKLSLDRFRAYY
                     KWFAYGQRLIHTLGVPAVSGSNCIMKREAYHSSGGFDQGLTCNEDSELAWRLDRMGYR
                     SYFASDLVVYAGDHRRLERGIMKKTLHSITRCTALYFDLIPERLKGSDWGYWSHINTN
                     RELQNGN"
BASE COUNT         1728 a         1734 c         1888 g         1920 t
ORIGIN      
        1 cacgtcggtt attacaggga gatgagcagg acgaatgtaa ggatagagac ctgtcccgtg
       61 gccgacacgg tcataaataa agctatatcc cggctctcga aagtactggc ttcggtcggg
      121 gacccgcggt atccgctcga taagatatat ttgtcgtccg acggggaaaa ggctcatata
      181 acactcattc ccaggtttaa aagcggtccc gcttcgctcg ggtcgctcgc gaaacatttg
      241 aggaggcacg aggagacgga agacgtctcc gtccatggag aggatgaggc cgggttcgag
      301 atctccgttg cggggttaag atttattgtc acgccctccg ttttcacgca gtccaatacc
      361 gccgttaacg aggccctcat ccggactgtc atcgaatggg cgggattgaa aggaaccgag
      421 accgtcctcg atctctattc cggcgcaggg aatttctcca taccccttgc gaagtcgtca
      481 aaacaagtta tttcggttga ggtaagcaaa aaagcggtcg aactcgctaa gaaaagcgca
      541 gggcttaacg gagcgaataa catcggtttt cataacgttt catgcgggga atatttgaac
      601 agcactgacg ccctcgccgg gtctgtcgat attgtagtgc tcgacccgcc gagagagggc
      661 gcgaaagaag ctgtggccgg aattaagaga ttggaggccg ggaaaatcat ttacgtgtcc
      721 tgtgaccccg ccacactggc gagggatatc aaattattta aagaagcggg ttatcggctt
      781 gagagattaa agccgttcga tatgtttcct caaacgtatc atatagaatc tctctcgctc
      841 cttgtcagag cctgacgagc tttactggat tcgattggag agattttaaa agactgcctg
      901 ccggtttttt gcgtccattt cggcactccc ctcgttcata tacgccgaat taatcatatc
      961 gtataccgac ctcgccatct ccttcctgga gctcgccgta ctcgggtcga ttttatccag
     1021 cacttttaat tcggcctcaa catatccgac ggacaggagt ttaaacaagt ggtcaaaaaa
     1081 tttcatgtcc ccgtaataat agatcaggtc tttattctta tcggtgagag cttccccgtc
     1141 gatactcgtg tatttgaggc agaggggcaa gatttcaacg cccgctttct gagcgatgct
     1201 taacagcggg gttttaaagg ggaggacccg gtccccgttc gatgtcgtcc cttctggaaa
     1261 aagggcgaca tttattccca attcgagaat ctcggagatc gattctatct cggtgcgcag
     1321 ccccgacctg ctcctcctct ctacgaagta ccccccgctc agctccgtcg ctttccccag
     1381 gaggagatcg cttttaacct cgtcgacgct cgcgatgaag gaaacaggga agaatgagga
     1441 tatgacaaat atatcaacgt acgaaaggtg attcgacagg atcagcacgc ttttaccgct
     1501 gccgataccg ttcccccgac tttgcaggct tacccggatg cctaacgcct tattcatgag
     1561 agccgaatga aaagacaagg ctctcgtgga ccagtttaat ttcctttttt tctctcttgc
     1621 tatagcccgg agtacagtcg tactcgtgaa ataggatgca actacgcaaa aaaaaagaag
     1681 agcttcgtgt actttctaat cattaggcgc cttgtatttc ttttcaaaag atttggtgat
     1741 gagatcgagg tcgagaactg tgaaaaagtc ggcgcacttg aacttcttat cgatcgccgg
     1801 ctccccgcag ataacggagc ccgccttgag atagctcttt agcagcggcg ggatgaaatc
     1861 ctctatcgat tccgtcctga tgccgaactt ctcgaaggcc tccagatagt aatcgagctc
     1921 ctttatcctg tgcttttcgt tgggatagac cctgaagttc tcgggggaca ggtagagctc
     1981 ttttatgtat ttataaataa ggctgatctc gacgatgttt gtcgtgccga tgctcgagca
     2041 tccgaaaagg tatttggcgt ttatcttctt gaagtattcc gataaccctt tccagagcag
     2101 cgcaatcccg gtgccgttcc gataatcctt gtggacgcat gccctcccga gctcgagctt
     2161 gatgccgggg gcggatttta tctggtccat cgagaactct gtctcggagt agaatttatc
     2221 cgagaaggtc gaggagatga acctgtacgt ccctactact atattctgtt ttttgtcgag
     2281 aatgatcaga tgatcgcaaa actggtcgaa cttgtcgatg tcggtcccgg agaagttttc
     2341 cttgttcagg gtctctctgt aaaaaacctc gtatctgagg ttgagggcct tctcgagctc
     2401 cgagctgttt tctactgtct ttatgaggaa cctgctgctt tcgatataga ggtgcacttt
     2461 gggtttgaac tgcctcagct tccatcggaa cgccttcttg agcacgtttg atgtggtgac
     2521 ggtatctttc aatgatatga cttttttggg cagcatgcgt atctccgcat tatgtgttaa
     2581 gtgattaata ccattttaat gtttaaacca tattaagtgt ctgtgaagtc tatgtttatg
     2641 gaatgcataa attatatcaa acatgctgat ataaagtcca gatttgacgg ataataggat
     2701 tctagagcgt ctctcttcga tcaagtctct ttaatacgtt tttaatattc ccttaatgtg
     2761 aggaaaatac aatacccgta tcgtaaattc cgctgtgtat tggcaaatct caagcgatcg
     2821 cgggcctgac cggcctctat gctcggccct cctatgtttg gaataactcg ttctatggaa
     2881 tataattgga tttcaggtgc ggcgggcaat ccgtgacgaa agttagctcc ggtcaggcgc
     2941 cggaatgttg tccgtgcggg acgggactag ccgtcatgac gactttattc ggccggaacc
     3001 tggctgagaa gggtgtttgt cactaaatag ggcatgaaag tattattcgg aattcagggt
     3061 accggaaacg gacatatctc caggtgcagg gagctgctca agcacctcct caggcgcgcg
     3121 gaagtggacg tgctcgtgag cggttatcat cacgaggtcg atctcgggtt cgaggtgaaa
     3181 tacaaactcc ccggtctggg attcacgttc gggaaacggg gggggataga ttactggaat
     3241 tccgtcagga acttcagtcc ctacaagctg atctcggaca tatacaagat accggtgcac
     3301 aggtacgacc tcgtgattag cgatttcgag ccgatcaccg catggtcggc gaaattgagg
     3361 agcgctccgt ccgtttctct gtctcatcag gcagctttcc tctctccgaa tatcccgagg
     3421 ccccggcagg agaaccgcat ccaggaactc attatcaaat ggtattcgcc ctcgccctca
     3481 catataggtc tccatttcca ggaatacgac gaattcattt tcacgcccat tataagggag
     3541 gaaatcagga acaccgacgt caggaacagg ggccattata ctgtctatct gccgtcatac
     3601 gactatagct tcctgtccgg cctattcagg agaatagacg taaaatggga agtcttttcg
     3661 aaacatcaca agggagagcc ctacagggac tcgaacgtca ccgtttatcc cgtgaacaac
     3721 ggtgaattcg tgagaagcct ttcggagtgc gagggtattc tctgcaacgc aggtttcgaa
     3781 acgcccgcgg aaaccctcta cctgggtaaa aagctgatgg tcgttccaat gaggagacag
     3841 tacgagcagc agtgtaacgc cgaggcgctc gaaaggctgg gggtacccgt tgtaagggac
     3901 ttgggaaatg agtttgaaaa taaattgtgc gattggataa actcgagttt cgcattcaac
     3961 gccgactaca agaacaacct tccgctaatc gtggagagga tattcgacag ctcctcgcgt
     4021 gcggacgctc tgaacgacta tatacaggac cccggcctgg agaacgaagg cgcgggtgcc
     4081 gcggactgaa aaggaagggt tcgaaaccgg agtgccgcgg actgaatcag cggatccgtt
     4141 tctaagacac ttttagcttt ttatcgccgt tattcgtaat gaacgagagg ttgacgagct
     4201 tctcggctat gttcttatac aactcgccta tcttaaggag attcagtact tcgtctatat
     4261 tgtgccagtc ctgtatcagg tatgtaagca taattctctt gtgctgatcc tccacggatt
     4321 cgattaatga atagtcctct tcgagttttt tcgtgtcgag cttttcgcgc cagttttcgt
     4381 tctcgacaag tatcctgatg tgggggagca ggatgtcgag catctgtttt gtatcgaaat
     4441 cggtgcacat aggctttttc gatttgaggt tttccatcag atgtactgca cacatgaatt
     4501 tctgaataat ggataacatg agcctgagaa cggtaacatt gttcggatta tgtttcttcc
     4561 tgctcttttc tatttcggtt tcgatcttga gctttagatg aatgaggtcg ttcctgagtg
     4621 attcgagctc cccttcgttc ctggagttga tataattctt cgatttcgat atgagccgct
     4681 caaatctgct ttcaagcaga cttctgtcca gattatccgt ttccatttca cccctccccg
     4741 gaaattgatg gggttattct atatgtcttt tgttaagatt gtgtgaagat tgcgttaaga
     4801 caaggtttca atctttgcag atcgatcccg atggtgagga atagaagata agccgcacga
     4861 tattgaagaa aataaaaaag ggcgagtcac tgcgggcgcg gactcgccct tagctttgag
     4921 agtgcccggt gagagaggcg ggcaatcaaa tagcagatga aaagatacta gcgctctttt
     4981 tttaagatgg ttttatgcct ccgttaagaa atagttaaat cggcatttga atatcgctgt
     5041 gtttattcgg gtcggcggtc ccgtactgca gcggtcgatg gcggggagaa aatttaccgg
     5101 atcacatgag gtgatctatg aattggtctg cgatggattt ccagctctgg ctgcgggcgt
     5161 agtcaagcgc atttcgtgac aggccgtcca gattgcgaaa ggccttctcg atgttctctt
     5221 cgaggctctc tcccaggtat cccgtaactc cgtcctgaat aatgtcgttc gggctcggga
     5281 catcatatgc gacgaccggc aggccgcagg ccatggcttc gagaatgacg agtccgaatg
     5341 tatcgaacat gctcgggaat acgaacacct gcgcctgcgt gtatatatcc ttaagctcgt
     5401 tgtgcgggac gtatccggcg aattcgacgt cgggatacct ggctttgagc gtgttcaggt
     5461 atggtccgtc gcctacgaga attttcctgt atcccggaac cctgcagaag tcgtcgagat
     5521 tcttgtcctt gctcacccgt gcaacggtaa tgatggtttt ttcgcctgag gttttttccc
     5581 cgtcccccgg attgaagagc cgtgtatcca cgccacgccc ccaaacgatg cagtttttga
     5641 ggccgagctg tccggctatg ctcttggtgg ttacaagaac ctttcggctg cacctgtgga
     5701 accacctcac gtagagccag gtcaggaacg tgggcactcc gtaaagtacc cagccgtagt
     5761 cggcgagcct cgtatgatag gaggtattgt agttgatttt atttttcctg cagaactgcc
     5821 tcatggcgag gccgaggctg ccctcggtcg cgatatgcac cctgtcaggc aggtgctcgt
     5881 cgtaatatct gttgacgatc tgatttgcgt ttttcagaat cgggatattc ccctggagct
     5941 tgagaggcag gagctccgtc ccttctatat aggggttgag gacggtcact tcgtggtcct
     6001 tcctgattaa attcgttata tagctccatg tatgtacgac gccgtttacc tgaggatgcc
     6061 aggcatcagt aacgagcatt atcttcatct tgttctgctc ccgcacaggt actacgtttg
     6121 aatgtggctt cagtgctcaa attatattcg cgaggtaaag gatgattatt actgtagcag
     6181 ctccgaggaa actgcccgcg agggtctgag atatcgtgtg ccttccgagc ctgatacggg
     6241 accaggcgat tatcggcaca ataagcgtaa ggggaagcac gttttctccg tacagggcca
     6301 ctgtgaggaa agcgagtccc gcggctgagg tacagtgacc gcttattttc catttaagcg
     6361 taatgacgaa gacgagcagt atctggagca gggccgcagc ggttactata agcatcagtt
     6421 tcggcgcgcc ccctagatac attacgagaa agatgattgc tgcataggcg aatatagcga
     6481 acagcggccg cgcgcgttcc tctcgtatgt tcatgtggaa atcgctcacc ttccctctgt
     6541 gcacgagcgt aagcacgaat atggtggggg gaattataag tagggcgata aagaccagga
     6601 tccagaatag agaggattcg ctcctagtag caagggcggt cacgacaatg catgcgagcg
     6661 ctatgagcgg gggactgaag aggttcgaaa ttaaccgggc cgacctgtcg cgccagtcga
     6721 gcggtattaa tatcggctcc gttgtctccc ggtaatattt tttctcagtt tccattttgc
     6781 agttcccggt tcgtattaat atgcgaccag taaccccagt cgcttccctt aagcctctcg
     6841 gggataaggt cgaagtagag cgccgtgcac ctcgtgatcg aatggagagt ctttttcata
     6901 ataccccgtt ctagccgcct gtggtctccg gcataaacga caagatcgga agcaaaataa
     6961 gatctatacc ccatgcggtc taaacgccac gcgagctcgg agtcctcgtt gcaggttaac
     7021 ccttgatcaa atcctccgga cgagtgatag gcttccctct tcataatgca attggagcct
     7081 gacaccgcag gcacgccgag cgtgtgtatg agacgctgac cgtatgcgaa ccacttatag
     7141 taggcgcgaa agcggtcgag ggagagtttg ggtccgtata tgaggtcgca ttccgcgagg
     7201 ttcccgacgt taataaagta atcccgggaa aatactatat cggcatcggt aaagagcagc
     7261 cattgggtaa
//
LOCUS       DASSJH010000059         6867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_14372, whole genome shotgun sequence.
ACCESSION   DASSJH010000059 DASSJH010000000
VERSION     DASSJH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14372"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..671
                     /locus_tag="VFJ67_01270"
     CDS             84..671
                     /locus_tag="VFJ67_01270"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET7289039.1"
                     /translation="MAAEKNQIDHIMSRLGEKIKNMRTERGLSLKELAHKSGISAAAI
                     HKIESNGITPTITTMMKISDALGKKVSHFIEEKAEHNDVVFVSSRDREPILTFKKGLE
                     LQGISAKQYGDFMMTAAYSVLEVGASSGKTPMKHRGEELVYCINGKVEFQVYDNTYTL
                     GPGDSIHFRTLLEHKWRNVGQSKAKLLWILAVPPS"
     gene            696..1991
                     /locus_tag="VFJ67_01275"
     CDS             696..1991
                     /locus_tag="VFJ67_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET7289040.1"
                     /translation="MSGKGGETVGGRYMGERIGEANSRALERLLAAEPVLMGVGTALS
                     SIPGMTPDTILHAGPPITWSRMCDPMKRAVRGALIFEGLASDDKSAEDLLKSRKIKLS
                     PNHHHDSVGPMTGIISPSMPVIVTKDQTHGRTVYSTFNEGGGKVLWFGSVGEESIRRL
                     RWMKDVFAPVMKKAIAMTGSIPVWSILAQGIQMGDECHNRHAASTNLLLKIIAETLFR
                     LDLPRERALEVYRFIAGNSHFHLNITMTACKLAMDAAHGIEDSTIVTSMSRNGTDFGV
                     RVSGLGDAWFTAPAPYLLDALYNPGYAARDGAPDIGDSSIIETMGLGGFAIAASPSMA
                     AFAGGGVAESISITKKMQLITTLKNPKFGIPALDFEGTPTGIDLRKVVETGILPSINS
                     AVVHKSTGAGQIGAGIVKAPFECFLKAVTAYGKRYDLAA"
     gene            2028..3581
                     /gene="fdrA"
                     /locus_tag="VFJ67_01280"
     CDS             2028..3581
                     /gene="fdrA"
                     /locus_tag="VFJ67_01280"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HET7289041.1"
                     /translation="MAIASIIKKNHYQDSLKLMQLSRKLSALPGIKKASAIMATEANL
                     KMLREAGLIRELPPSAGANDLIVAVEAESDSKAREALDSLDSLVSRAGASGPASAGPE
                     KSLDSALNVFPGANLAFVSVPGEYAKLECAKAIEKGMHTFLFSDNVTIDEEIELKKRA
                     SARGLLVMGPGCGTAIINGTGLGFANVINKGPVGVIAAAGTGMQEVTSLLSNLGSGIT
                     HAIGVGGRDLSEAVGGIMTLDALGILGGDENTKVLLVVSKPPSEKVAGIILAAIRDTG
                     KPAVVCFLGHEMKAPVDEKIKLVSTLEEAAYAALGLAAGSGKKKAPRSSDGWKKEARK
                     LVRSRKRGQRYLRGLFSGGTLCYEAQHILTRELGDIYSNAPLYKKNRLRDSNESVGNT
                     CVDLGEEEFTVGRPHPMIDATLRSERLLKEAVKAEVAVILFDVVLGYVASPDPAGDLI
                     PAIKAARAAAGKKGRSVAFVAHVCGTQADPQGLRGQEAKLRKEGVMVFPTNAQASRAA
                     GWIAAGGRD"
     gene            3604..4542
                     /gene="arcC"
                     /locus_tag="VFJ67_01285"
     CDS             3604..4542
                     /gene="arcC"
                     /locus_tag="VFJ67_01285"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET7289042.1"
                     /translation="MKAAVVAFGGNALMDHKGKSTYVEQIMKTDKMCRKLMGLFDMGY
                     RVVITHGNGPQVGNFLMQQECLSASIPPMPLDVCNAMTQGQIGYMIEQRLRNLFLEKK
                     MSNPTVAFVTQVVVAEDDPAFMNPTKFIGPFFTEEESEKQKKREGWIMKEDSGRGYRR
                     VVPSPRPIDIVEKREISEMADRGYVVIACGGGGIPVLRDKKGKLKGVAAVIDKDFAAQ
                     KLATLIGAEILVLITPVDRVAINFGTPAEKYLSRIRLEEAERYHAEGHFPPGSMGPKI
                     EAAISFLRAGGKKAVITSVEKMDTAINGKGGTEITQ"
     gene            4705..5196
                     /locus_tag="VFJ67_01290"
     CDS             4705..5196
                     /locus_tag="VFJ67_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HET7289043.1"
                     /translation="MAVNRVKADRPEKGKALVNYEEKLYPDHKAKPGEKALFLIHSVP
                     YEGSVAGINMLTAIRTKKKGYDVTVLFYGPASGIPVYRGWPNVGDDGYGAGIQLYPNL
                     VNRMLSEGISVLACRFSAAALLGQNESSFIEGVTPIHPTDILDIVIEHHRAGALIFST
                     WTV"
     gene            5319..6440
                     /locus_tag="VFJ67_01295"
     CDS             5319..6440
                     /locus_tag="VFJ67_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HET7289044.1"
                     /translation="MDSITLKVELQSYGLRIPDARDVRSGGAGPTGGIHLKILSGGEA
                     NIPVVGDFVRSSPYHLDQINGEYWIFKDGVAVAQVELMDGPKFHGHQTSAGIPMQRIG
                     LLHCPTTFATTLLQTCDFWEDERRCRFCGIELTLKDRSTVGFKNAKSLVETLMMAKEL
                     DGVTNVVFTSGVAPDEEKALAKYAEICREVKAATGLPIQLQIIPPEDLSWLGRLRDSG
                     VDSLGVHIETFDPDVFRTVTPGKARIGLDKYIETWKEAVRVFGRWQVSTYILVGLGER
                     LETVIEGAALCAEIGVYPFIVPFRPIAGTPMENVAPPRPEVMRHVYTEAAKILSRYDI
                     GVNKVSKAGCVSCGECSALHDFEKSMGADGVRFKKFRKQ"
     gene            6570..>6867
                     /locus_tag="VFJ67_01300"
     CDS             6570..>6867
                     /locus_tag="VFJ67_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7289045.1"
                     /translation="MSSDNLRIIIIGGGAGGISAASTSKSVAPGASVTVFTEYEDVAY
                     SPCGIPFVLGKEIPDFERLFLQTPEHYASIGIYLILKTAVTGIDHNGRTVSVGAE"
BASE COUNT         1622 a         1788 c         2251 g         1206 t
ORIGIN      
        1 aggaaacgaa aaagaaatgt tctgggattt cggacgggag cccctcatcg gggtgagccc
       61 gaaaaacggt gaaaaggtga aaaatggcgg ctgagaagaa tcagatcgat cacataatgt
      121 ccaggctcgg agagaagata aagaacatga ggaccgaaag ggggctctcg ctcaaggagc
      181 tcgcccataa atcggggata tccgccgcgg cgatccacaa gatcgaatcc aacggcataa
      241 cgcccacgat cacgacgatg atgaagatat cggacgcgct cgggaagaag gtgagccact
      301 ttatagagga gaaggccgag cacaacgacg tcgtcttcgt ttcgtccagg gacagggagc
      361 cgattctcac gttcaagaag ggactcgagc tccagggcat atcggcgaag cagtacgggg
      421 acttcatgat gacggccgct tattcggtgc tcgaggttgg ggcatcgagc ggtaagaccc
      481 caatgaagca caggggcgaa gagctcgttt actgcataaa cgggaaggtc gagttccagg
      541 tgtacgacaa tacctacacg ctcggcccgg gcgacagcat acatttcagg acgctgctcg
      601 aacacaaatg gagaaacgtg ggccagagca aggcgaagct gctctggata ctggcggtgc
      661 cgccctcgtg acgggcggaa ggagctgaag gaccgatgtc cggaaaaggc ggagagaccg
      721 tgggcggacg gtacatgggt gaaaggatcg gggaagcgaa ttcgcgcgcg ctcgaaaggc
      781 tgctcgcggc ggagcccgtc cttatgggcg tcggcacggc gctctcctcg atacccggca
      841 tgacccccga cacgatactc cacgcggggc ctccgattac gtggagccgg atgtgcgacc
      901 ctatgaagag ggcggtcagg ggcgctctta tattcgaagg ccttgcctct gacgacaaat
      961 ccgcggaaga tctcctcaaa tccaggaaga taaaactctc gcccaaccat catcacgatt
     1021 cggtgggccc gatgacgggg attatctcgc cttcgatgcc cgtcatcgtt actaaagacc
     1081 agacgcacgg caggacggta tattcgacgt tcaacgaggg cggcggtaag gtgctctggt
     1141 tcggctccgt gggcgaggag tcgataaggc gtctcaggtg gatgaaggac gtgttcgcgc
     1201 ccgtgatgaa gaaggcgata gccatgacgg gcagcatccc cgtgtggagc attctcgccc
     1261 agggcataca gatgggggac gaatgccaca acaggcacgc cgcctcgacg aacctcctgc
     1321 tcaagataat tgctgagacc ttgttcaggc tcgacctccc ccgcgagagg gcgctcgagg
     1381 tttacagatt catagcgggg aacagccatt tccacctcaa cataacgatg accgcatgca
     1441 agcttgcgat ggacgcggcg cacggcatag aggattcgac gatagttacg tcgatgtcac
     1501 ggaacggaac ggatttcggc gtaagagtta gcggcctggg cgacgcatgg ttcacggccc
     1561 ccgcgcctta cctgctcgac gcgctttaca atccggggta cgccgcacgg gacggcgcgc
     1621 cagacatagg ggacagctcg ataatcgaga ctatgggcct gggcgggttc gccatagcgg
     1681 cctctccgtc catggccgct ttcgcgggag ggggagtcgc cgagtctata agcatcacga
     1741 agaaaatgca gctcatcacg acgctgaaga acccgaagtt cggcataccg gcgctcgatt
     1801 tcgaagggac tcccacgggc atagacctca gaaaggtggt cgaaaccggg atactgccca
     1861 gcataaacag cgccgtagtc cacaagagca cgggcgcggg acagataggc gcgggaatag
     1921 taaaggcgcc ctttgagtgt ttcttgaagg cagtgacggc gtacggaaaa agatacgatc
     1981 tcgcggcctg agggctgcgc ggatttgcat taaacgggtg aatgtatatg gcgatagcca
     2041 gcattattaa aaagaaccac taccaggact cgctgaagct catgcagctg agcagaaagc
     2101 tgagcgctct gcccggcata aagaaggctt ccgcgatcat ggcgaccgag gccaatctca
     2161 aaatgctgag ggaggcgggg ctcattcgcg agctccctcc gtctgcgggc gcgaacgacc
     2221 tcatagtcgc cgtcgaggca gaatcggatt caaaagcgcg cgaggcgctg gattctctcg
     2281 attcgctcgt ctcccgggcg ggggcatccg ggcctgcgtc ggccgggccc gaaaagagcc
     2341 tcgattctgc gcttaacgtg tttccgggcg cgaacctcgc gttcgtttcg gtcccggggg
     2401 aatatgcgaa gctagaatgc gcgaaggcga tagaaaaggg catgcacacg tttctattca
     2461 gtgacaacgt aactatagac gaagagatcg agctcaagaa aagggcttcc gcgcgcggct
     2521 tgctggtcat gggccccgga tgcgggactg cgataataaa cggtacgggg ctcgggttcg
     2581 cgaacgtgat taacaaaggc ccggtaggcg tcatcgcggc cgcgggaacg ggtatgcagg
     2641 aggtgacgag cctcctgagt aacctcggct cgggcatcac acacgctata ggcgtcggcg
     2701 gaagggacct ctcggaagct gtgggcggaa taatgacgct cgacgcgctc gggatactgg
     2761 gcggggacga aaacacgaaa gtactgctcg tcgtctcgaa gcctccatcg gaaaaggtgg
     2821 ccgggataat tctcgcggcg ataagggata ccgggaagcc cgccgtcgtg tgctttctcg
     2881 gacacgaaat gaaagcaccg gtcgatgaaa agataaaact ggtctcgacg ctcgaagagg
     2941 cggcgtacgc tgcgctcggt cttgcggcgg gaagcggcaa aaagaaagcg ccccgctcaa
     3001 gcgacggctg gaaaaaagag gcccggaagc tcgtccggtc gcgtaagcgc ggacagcgct
     3061 acctgagggg actcttctcg ggcggcacgc tctgctacga ggcgcagcat attctaacgc
     3121 gggagctcgg ggacatatac tcgaacgcgc ctctctacaa gaagaacagg ctcagggact
     3181 caaacgagag cgtgggcaac acgtgcgtcg acctcggcga ggaggagttc acggtaggaa
     3241 ggccgcaccc gatgattgac gcgacactga ggagcgagcg tctcttgaaa gaagcggtca
     3301 aggcagaggt ggccgtaata ctcttcgacg tcgtgctcgg ttacgtggcg agcccggacc
     3361 cggccggaga ccttattccc gcgataaagg cggccagggc ggcggcaggg aaaaaaggac
     3421 ggtctgtcgc attcgtcgcg cacgtgtgcg ggacgcaggc cgatcctcag ggtctgcgcg
     3481 gacaggaggc gaagctcagg aaggaaggag tgatggtctt tccgacgaac gctcaggctt
     3541 cgagagcggc aggatggata gcggcgggag gaagagattg aaagacaccc acggggggaa
     3601 aatatgaaag cagcggtagt agccttcggg ggtaacgccc tgatggacca taaaggcaag
     3661 agcacttacg tagagcagat tatgaaaacg gacaagatgt gcaggaagct catgggcctg
     3721 ttcgacatgg gatacagggt cgtgatcact cacgggaacg gcccgcaggt ggggaatttc
     3781 ctcatgcagc aggagtgcct ctcggcgtct ataccgccca tgccgcttga cgtctgcaac
     3841 gcaatgacgc aggggcagat cgggtacatg atagagcaga gactccgcaa cctgtttctc
     3901 gagaagaaga tgagcaaccc gacggtcgcg ttcgtgacgc aggtggtggt cgcggaagac
     3961 gacccggcgt tcatgaaccc taccaagttc ataggccctt tcttcacgga ggaggagagc
     4021 gaaaagcaaa aaaagagaga gggctggatt atgaaggagg attcggggcg cggctacagg
     4081 cgggtagtcc catcgcccag gccgatcgat atagtggaaa aaagggagat atccgaaatg
     4141 gccgacaggg ggtacgtcgt tatcgcgtgc ggagggggcg gcatacccgt gctcagggat
     4201 aagaaaggga agctgaaggg tgttgcggcc gttattgaca aggacttcgc agcgcagaag
     4261 ctggcgacgc tcataggagc tgagatactc gtccttatca cgcccgtcga cagggtcgca
     4321 ataaatttcg gtacgcccgc cgagaagtac ctctcgagga tcaggctcga ggaggccgag
     4381 cgttatcacg cggagggcca tttccctccg gggagcatgg ggcccaagat cgaggccgcc
     4441 ataagcttcc tccgcgccgg aggaaagaag gcggtaatca cgtccgtcga gaagatggac
     4501 acggccatta acggaaaagg ggggacggag ataacgcagt aagcgccctc tccggagata
     4561 aagtatgcga ttgattatgc cgttgtttat tgtatcatgg ggttgtgggg actcgggaga
     4621 agaaccttcg tataaaacag ggatgaagcc ccgggcttgc gctcagggga ttgaatctaa
     4681 ataaaccaca ggaggtgtac tgctatggca gtaaacagag taaaagcgga caggcccgag
     4741 aagggtaaag cgttggttaa ttacgaggag aagctctatc cggaccataa ggccaaaccg
     4801 ggggagaaag ccttattcct catccactcg gtcccttacg agggctcggt agccggcatc
     4861 aacatgctga cggcgataag gacgaagaag aagggctacg acgtgacggt gctgttttac
     4921 ggccccgcat cgggtatccc cgtttacaga ggctggccga acgtcggcga cgacggctac
     4981 ggcgcgggca tacagctcta cccgaacctc gtcaacagga tgctgagcga aggcattagc
     5041 gtccttgcat gccgtttctc ggccgcggct ctcctcggcc agaacgagtc gagcttcatc
     5101 gagggagtaa cgccgatcca cccgacggac atactcgata tagttatcga gcaccacagg
     5161 gccggggctt tgatattcag cacgtggacc gtgtaaggcg ctgccgcctt atgctttcag
     5221 ttcgtctgtg agcggctcgt acgcggacgg cggttttaca ggtttgcgag ggtgggcctg
     5281 caagcggttt ttgtctaacc aattcaaggg ggcacgttat ggattcaata acccttaaag
     5341 tggagttgca gagttacggt ctgaggattc ccgatgctag agacgtcagg agcgggggcg
     5401 ccgggcctac ggggggtatc catttgaaga tactctcggg gggcgaggcg aacatccccg
     5461 tcgtcggcga tttcgtccgc tcgtctcctt accacctgga ccagataaac ggtgaatact
     5521 ggatattcaa ggacggggtc gccgtcgctc aggtggagct gatggacggt ccgaagttcc
     5581 acgggcatca gacgtcggcc ggcatcccga tgcagcgcat aggacttctt cactgcccta
     5641 cgacgttcgc caccactctc cttcagacgt gcgatttctg ggaggacgag aggaggtgca
     5701 ggttctgcgg catcgagctc accctcaagg acaggagcac agtcggtttc aagaacgcga
     5761 agagcctcgt cgagactctc atgatggcaa aggagctcga cggcgtgacg aacgtggtgt
     5821 tcacttcggg ggtcgccccc gacgaggaga aggctctcgc aaaatacgcc gagatatgcc
     5881 gtgaagtgaa ggcggcgacc ggcctgccga tacagcttca aataataccg cccgaagacc
     5941 tctcgtggct cgggaggctc cgggattcgg gcgtcgattc gctcggcgtg cacatagaga
     6001 cgttcgaccc ggacgtgttc aggacggtca cgccgggcaa ggcccggatc gggctcgaca
     6061 aatacatcga gacctggaag gaagccgtgc gcgtgttcgg caggtggcag gtgagcacct
     6121 atatactggt cggtctcggc gagaggctgg agacggtcat agaaggcgca gcgctttgcg
     6181 ccgagatagg cgtttacccg ttcatagtcc cgttcaggcc catcgcggga acgcctatgg
     6241 agaatgtcgc gccgccccgg ccggaggtga tgcgtcatgt ctataccgag gccgcgaaga
     6301 tactttcacg gtacgacatc ggcgtgaata aggtcagcaa ggcgggctgc gtgagctgcg
     6361 gggagtgctc cgcgctccac gatttcgaga agtccatggg cgcggacggg gtcaggttca
     6421 agaaattcag gaagcagtag cgggcgggtc gaggggtttg gttacagagc gtagtttcag
     6481 gcggagagaa ggcggtatcg gccgcccctc gggtggttct gtgcccttca cgggaagaat
     6541 ataaaacgag acgaaaaggg gtgtaccgga tgagcagcga taatttgcgg ataataataa
     6601 tcggaggagg cgcgggaggc atatcggccg cgtccacgtc caaatctgtc gcccccgggg
     6661 catccgtcac cgtgtttacg gagtacgagg acgtagccta cagcccgtgc gggataccgt
     6721 tcgtgctggg gaaggagatc ccggatttcg agcggctttt tctccagacc ccggagcact
     6781 acgcctcgat aggaatatac ctgatactga agaccgccgt caccggtatc gaccacaatg
     6841 ggcgcaccgt gagcgtcggg gccgaga
//
LOCUS       DASSJH010000060         8532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1529046, whole genome shotgun sequence.
ACCESSION   DASSJH010000060 DASSJH010000000
VERSION     DASSJH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529046"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..962)
                     /gene="murB"
                     /locus_tag="VFJ67_01305"
     CDS             complement(57..962)
                     /gene="murB"
                     /locus_tag="VFJ67_01305"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7289046.1"
                     /translation="MSLEREIARIGCEIDRMYPMSKYTSLRVGGLADLLVTPNNLGEF
                     RNLLGLLSGSGTPWVVLGAGSNTVVYDAGIEGVVVSTKKLKNIEITGDNKVFAETGAV
                     LGTILNRTIKAGLSGFEFAAGIPGTVGGGVFMNAGANGGEIKDVIETVWVWLDGKEIS
                     IDRKDLKFEYRKSYLPKGSVITRAKFGLTPGDPKEIERNVKEYMEKRNRTQPIKMSNT
                     GSIFKNPPEIPAGKLLDELGFKGLCVGGAKFSEMHANFIVNAGCASASDVLDLIEKAK
                     KEALDKRGVRLETEVRVIGKKKVGR"
     gene            complement(959..2326)
                     /gene="murC"
                     /locus_tag="VFJ67_01310"
     CDS             complement(959..2326)
                     /gene="murC"
                     /locus_tag="VFJ67_01310"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET7289047.1"
                     /translation="MYGKIKRIHFVGIGGIGMSGIAEVLLNLGYTVSGSDLKESDTVK
                     RLRDLGAVISIGHSGENVRGAGVLVISSAVNETNPEVVEARKSGIPVIPRAQMLAELM
                     RLRYGIAIAGSHGKTTTTSMIATVLSHGGLDPTIVVGGKVKTLGTNARLGKGSFMVVE
                     ADESDGSFLLLSPVISVLTNIDEEHLDHYKGLPELVEAFSTFADKIPFYGLSVFCADC
                     PRVREIAKNFEKRSVTYGFHPEAELRAENVSISGFHTEFEALLNGSPLGRISLNVPGR
                     HNAQNALAAVAVGMELGMGFDDIRDGLAEFSGIDRRLQIKGRGRDVLVLDDYAHHPNE
                     IRATIKAVRDSGLGRIVAIFQPHRYSRTKLLFDDFADVLQDIDVLYLMDIYPAGEEPI
                     EGVTSKSLYKSVKEKGHGNVHYSNGSENLVSSVLETIRPGDVVITLGAGNVWAVGEKI
                     AEEIA"
     gene            complement(2326..3396)
                     /gene="murG"
                     /locus_tag="VFJ67_01315"
     CDS             complement(2326..3396)
                     /gene="murG"
                     /locus_tag="VFJ67_01315"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET7289048.1"
                     /translation="MKVIIAGGGTGGHIFPAISVAEEILKRSGTNEVLFVGTKKGLEN
                     ELLSKRGFRVEHISARGIKGKGVGKSISALLAAFKGVSDSVSIIKEFRPDVVLGVGGY
                     VSGPAVLAASLRGIPTAICEQNGYPGLTNRILGKLVRKVFITFDESIKFFPRRKVVIT
                     GNPVREDILRSLNKKKDSDTITVLVFGGSQGAHKLNASVPGALSKLGRTDIKVIHQTG
                     DMDYDIVKNAYTDSGIEARVLRFIDDMAGAYAEADFVIGRSGAGTVAEITALGKPSLL
                     VPYPFATNNHQMENARVLERAGAAVIVEDRDASAENLQAALTKLLRKDKLNTMASHAL
                     ALGRPDAASVIVNEISKLSGEN"
     gene            complement(3393..4508)
                     /gene="ftsW"
                     /locus_tag="VFJ67_01320"
     CDS             complement(3393..4508)
                     /gene="ftsW"
                     /locus_tag="VFJ67_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET7289049.1"
                     /translation="MLDIFSSSKAKAYDIGVIVSAVFLTAVGVLMVYSTSSVYSMEMY
                     GNANYFLIRHGIYLAIGLVAMIVLMRVDYRLLRKFVYPAYLLGLALLVAVLVPGIGKE
                     VGGARRWIDLGLLSFQPSEFAKYILVLYLAHSLTKKRDKLDNFIVGFASHILIAGVYV
                     ILVLLEPDFGTATIMLVTLFCMLFIGEVKMKYLLPVGAVSVIFLILAVITKGYRMNRV
                     MSFLDPWQDPLGSGYQAIQSFIAFALGGVSGTGLGDSSQKLFFLPQAHTDFIFSIIGE
                     ELGFIGIVLVLAGFVVLLVRSLKVSLRAPDLFGCYLVFGCVVLIALQAGINMAVAVGL
                     FPTKGLTLPFISYGGTSLIATLSAVGVILNVSRSGMK"
     gene            complement(4483..5820)
                     /gene="murD"
                     /locus_tag="VFJ67_01325"
     CDS             complement(4483..5820)
                     /gene="murD"
                     /locus_tag="VFJ67_01325"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET7289050.1"
                     /translation="MELRDRKVLVVGVGKTGMETVKFLLGKGARVSVSDSSPAGEIAG
                     QVKTLESWGVSCETGGHTSETFLSSGTIVLSPGVPFGIPPVREALASCIEVISEVELA
                     SRFIRRPTIAVTGSNGKTTTSTLIARILEKNGRRVFLGANIGTPLIQIADDCDRYDIL
                     VLELSSFQLQGIESFRPDVSVILNVTPNHLDHHASFEEYAESKMKIFSNQTEHDWCIY
                     KADDPVIAGYVPAIKAGKIPFGAGDVKNGVSYDGSGIKYGSAYYDLSGMKLRGRHNVE
                     NVMAAVAASTVMGCDPRLIREAVLEFDPLPHRIEYVGEIKGAKFYNDSKSTSPDATLR
                     ALESLPPPIILIAGGKDKGVSFEPLRETIEKKVKLMVLMGESRFRMEKDLGGKVGATL
                     AASLEEAMDKTLEQLAPGDSVLFSPACSSFDMFRSYEERGRRYKDLVRHIQLV"
     gene            complement(5872..6981)
                     /gene="mraY"
                     /locus_tag="VFJ67_01330"
     CDS             complement(5872..6981)
                     /gene="mraY"
                     /locus_tag="VFJ67_01330"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7289051.1"
                     /translation="MFHLLYLLKGDFILFNVFRYITFRSFGAGVTAFLISIILGGFFI
                     NFLRKRQFQENIRDDGPASHKTKAGTPTMGGAFIILSILISSLLWLNLTSEIVWSVLL
                     FTMSYALIGFLDDWLKYSKKKGMKARVKFSLQILFGILFVLLVMSDLDGFTMSLSANQ
                     VAEYSFTSVVFPFIKKAVINLGWLYIPFALIVVVGASNGVNLTDGLDGLAIGSIAVAA
                     ATYVIFAYLSGHAEFSKYLQIPYIPEGGELAVYLAAVGGACLGFLWYNSHPAEVFMGD
                     VGSLALGAAIGSVALILKQEILLILVGGVFVAEAMSVIIQVASFKLTGKRVFRMAPLH
                     HHFELKGWSESKVVIRFWIISVVLSLFALSTLKLR"
     gene            complement(6993..8357)
                     /gene="murF"
                     /locus_tag="VFJ67_01335"
     CDS             complement(6993..8357)
                     /gene="murF"
                     /locus_tag="VFJ67_01335"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET7289052.1"
                     /translation="MLGLDFVLNSTSGKLVSGGNGISFSGVSTDSRNISSGEIFFALR
                     GENYDGHEYVDEAIKKGAGGAVIEQNSIAHEAGKLLIRVPSTLRALGDLASQWRKSFP
                     QLKLAAVTGSNGKTTTKEMAWSMVSRKYKTLKNTGNFNNLIGLPLTLFKLDASYKAAV
                     VELGMNDFGEIKRLAEIALPDTGAITNIGRAHLEKLGGIEGVARAKGELVEGFTGENV
                     FVVNADDPRTAEIAKKTDCKKITYGIKSDGADITARNIIQDGFSGIDFLIRTKNEEFP
                     VRIKGIGVHNVMNALCASGIALSMGCGKDEIIAGLEGFTPGYMRLEVLESPSGFRVIN
                     DTYNANPDSMRSAADELMGLKGGGRAIAVLGDMLELGEKSAGEHYGVGEYLSASGVDY
                     VIACGNFGRTLLEGAGGGPNCFYAESHEEAANIVREIAGPGDLVLVKGSRGMRMEEVT
                     KKLV"
     gene            complement(8373..>8532)
                     /locus_tag="VFJ67_01340"
     CDS             complement(8373..>8532)
                     /locus_tag="VFJ67_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET7289053.1"
                     /translation="KEAIRKAICMAAEKDTVLIAGKGHEDYQIIGTTKYPFDDRTAAL
                     EILKEMLN"
BASE COUNT         1755 a         2533 c         2241 g         2003 t
ORIGIN      
        1 caaccaccac gaaagacgaa atggcgatta ttactttctt gtacgggaat ttcgcgctat
       61 cttccaactt ttttcttccc tattactctg acttcagtct cgagcctgac tcctctcttg
      121 tcgagcgctt ctttcttcgc cttctcaatg agatcgagca cgtcgctcgc gctcgcgcat
      181 ccggcgttga ctataaaatt ggcgtgcatc tccgaaaact tcgctccccc gacgcacaag
      241 cccttgaacc cgagctcgtc caggagcttc cctgccggta tctcgggcgg gttcttgaaa
      301 atggaccccg tgttcgacat ttttatcggc tgggtcctgt ttcttttttc catatattcc
      361 ttgacgttcc gctctatctc tttcgggtcg ccaggggtca gcccgaactt cgccctcgtg
      421 atcacgctcc ctttgggcag ataactctta cggtactcga acttcaagtc tttcctgtcg
      481 atggatattt ccttgccgtc gagccagacc catacggtct cgatcacgtc ctttatctcc
      541 cctccgtttg cgcccgcgtt catgaacaca ccgccgccta cggtacccgg aatcccggcc
      601 gcgaactcga aacccgagag ccctgccttt atcgtacggt tgagtatcgt gccaagcacg
      661 gcgcccgtct cggcgaaaac cttgttatcg cctgtaatct cgatattttt cagctttttg
      721 gtggatacga caaccccttc gatcccggcg tcgtaaacga ccgtgttcga tcctgccccg
      781 aggactaccc acggcgtgcc ggacccggat aaaaggccga gcaggttcct gaattccccg
      841 aggttattgg gagtgacgag gaggtcggcg agacccccca cacggagcga cgtgtacttg
      901 ctcatcgggt acatccggtc gatttcgcag cctatgcgcg cgatctccct ctcgaggctc
      961 atgctatctc ctcggctatc ttttcgccca cggcccatac gtttccggcc ccgagcgtga
     1021 tcacgacgtc cccgggcctg atagtctcga gcacggagga tacgaggttc tcgctgccgt
     1081 tcgagtagtg gacgttaccg tgtcccttct cctttacgga cttatagagg gatttggacg
     1141 ttacgccttc gatcggctct tctcccgcgg ggtaaatgtc catgagatag aggacgtcta
     1201 tgtcctggag cacgtcggcg aagtcgtcga agaggagctt cgtcctcgaa taccggtggg
     1261 gctggaaaat ggcgactatc ctgccgagtc ccgagtccct gaccgctttt atggtcgctc
     1321 ttatctcgtt cgggtggtgc gcgtaatcgt cgaggacaag gacgtccctg cccctgccct
     1381 ttatctggag ccttctgtct atgccgctga actcggcgag gccgtctctg atatcgtcga
     1441 agcccatgcc gagctccata ccgacggcca cggcggcgag agcgttctgc gcgttgtgtc
     1501 tgccggggac gttaaggctt atcctgccga gcggggagcc gtttaaaaga gcctcgaact
     1561 ccgtatggaa tccggatatg ctgacattct ccgccctcaa ttcagcctca gggtgaaaac
     1621 cgtacgtgac ggaccttttt tcaaaattct tcgctatctc ccttaccctc ggacagtccg
     1681 cgcagaagac ggagaggccg taaaagggaa tcttgtccgc aaatgttgaa aatgcctcca
     1741 cgagctccgg gaggcccttg taatgatcga ggtgttcttc gtcgatgttg gtaaggaccg
     1801 atatgacggg ggagagcagg agaaaagagc cgtcagactc gtcggcctcc acaaccatga
     1861 agctgccctt gccgagcctg gcgttcgtcc cgagcgtctt gaccttgccc ccgacgacta
     1921 tcgtggggtc gagcccgccg tgggaaagca ctgtcgctat catagaggtc gtggtcgttt
     1981 tgccgtggct cccggcaatt gctatcccgt acctgagtct catgagctct gcgagcatct
     2041 gggcccgcgg aatgacgggg atgccgctct tcctcgcttc gacgacctcg gggttcgttt
     2101 cgttcacggc ggacgatatg actaacacgc cggcgccccg gacgttctcc ccgctgtgac
     2161 ctattgaaat aaccgcgccc aggtctctca ggcgttttac ggtatccgat tctttaagat
     2221 cggagccaga gaccgtataa cccagattga gaaggacctc ggcgataccg ctcatcccta
     2281 tgccgcctat acctacaaaa tgtatccgct tgatttttcc gtacactaat tctctccgga
     2341 gagcttggat atttcgttca caataactga agccgcatcg ggcctgccga gagcgagcgc
     2401 gtgtgaagcc atcgtattta gtttgtcctt gcggaggagt ttggtcaatg ccgcctgaag
     2461 attttcagcg gacgcgtccc tgtcctcgac tatcaccgcc gctcccgcgc gctcgagcac
     2521 acgggcgttc tccatctgat ggttattggt cgcgaacggg taagggacca ggagcgaagg
     2581 cttcccgagc gcagtgatct cggcgacagt acccgcgccc gaccttccga tcacgaaatc
     2641 ggcctcagcg tatgctcccg ccatgtcgtc tatgaacctg agcacccgcg cttcgatccc
     2701 agagtccgtg taggcgtttt taacgatatc gtaatccata tcgcccgttt gatgtatcac
     2761 ctttatgtcc gtcctcccga gcttcgaaag ggctccgggg acagaggcgt tgagtttgtg
     2821 agcgccctgg ctgccgccga acacgagtac ggttatcgta tccgaatctt tttttttgtt
     2881 caggctgcgc aatatatctt ccctaaccgg gttgcccgtg atcacgacct tcctgcgggg
     2941 gaagaatttt atgctctcat cgaacgttat gaacaccttg cgcacgagct tcccgagtat
     3001 gcggttcgtc aaaccggggt agccgttctg ctcgcatatc gccgtgggta tgcccctgag
     3061 tgaggccgca agcacagcgg gccctgaaac gtagcccccg acgccgagaa cgacgtcggg
     3121 tctgaactct tttataatcg acaccgaatc ggagaccccc ttaaatgcag ctaaaagcgc
     3181 ggatatgctt ttcccgacgc ctttaccctt tatgccgcgg gcgcttatgt gctcgaccct
     3241 gaaacctctt tttgaaagca gctcgttttc caaccctttt ttcgtaccca cgaaaaggac
     3301 ttcgttcgtc ccgcttctct ttagtatttc ttccgcgacc gagatcgccg gaaatatatg
     3361 tcccccggtt ccccctccgg cgatgatgac cttcatttca tacccgatct ggagacgttg
     3421 agtatcaccc ccacggccga gagggtcgct atgagagacg tgcctccgta gctgataaag
     3481 ggaagtgtga gtcctttagt cgggaagagt cccacggcaa cggccatgtt gatccccgcc
     3541 tgaagcgcga tcaataccac gcaaccgaaa acgaggtagc atccgaagag gtcgggagcc
     3601 ctgagggaga ccttgaggga tcttacgagg agcacgacga atcccgcgag gacgagcact
     3661 atgcctataa agcccagctc ttcgcctatg atggagaata tgaagtccgt gtgcgcctgg
     3721 gggaggaaga agagcttctg cgagctgtcg ccgagacctg tccccgagac gcctccaagc
     3781 gcgaacgcga tgaacgactg tatggcctga tagcccgagc cgagagggtc ctgccaggga
     3841 tcgaggaacg acatgacgcg gttcatcctg tagcccttcg ttatgacggc aagaataagg
     3901 aatatgacgg aaaccgctcc cacgggcagg aggtacttca tcttgacctc gccgatgaaa
     3961 agcatgcaga agagcgtgac gagcatgatc gtcgcagtgc cgaaatcggg ctcgagaaga
     4021 acgagtatca cgtaaacgcc ggctatcaaa atgtgcgagg cgaagccgac tatgaagtta
     4081 tcgagcttgt cccttttctt cgtaagtgaa tgggcgaggt agaggacgag tatgtatttg
     4141 gcgaactcag agggctggaa gctgaggaga ccgaggtcga tccacctcct ggctccgccg
     4201 acctctttgc caattccggg gacgagcacg gcgactaaaa gcgcgaggcc gagcaggtat
     4261 gccggataga cgaacttcct gagaagacgg taatcgaccc tcatgagcac tatcatcgcc
     4321 acgaggccga tggccaggta tataccgtgg cgtatgagga agtagttcgc gttcccgtac
     4381 atctccatcg aatatacgga gctcgtgctg tacaccatca gaacaccgac agccgtaaga
     4441 aatacggccg agacgattac gcctatatcg taagcctttg ccttagacga gctgaatatg
     4501 tctaacaagg tccttatacc tccttcctcg ttcttcgtac gaccggaaca tatcgaaact
     4561 cgagcaagcg ggcgagaata gaacgctgtc tcccggagcc agctgctcga gtgtcttatc
     4621 catcgcttct tcaagcgacg cggcaagcgt cgcgcctacc ttgccgccca gatctttctc
     4681 catcctgaac ctggactctc ccatgagcac catgagcttg accttttttt ctatagtctc
     4741 ccttaagggc tcgaagctca cgcccttgtc cttcccgccg gcgatgagaa ttatcggcgg
     4801 gggcaggctc tcgagagcgc gcagagtggc gtcggggctc gtggatttgg aatcgttgta
     4861 aaatttcgcg ccttttattt cgcctacgta ctcgatcctg tgcgggagcg gatcgaactc
     4921 gagcacggct tcgcgtatga gacgcgggtc gcaccccatc acggtcgaag cggctactgc
     4981 cgccatcacg ttctcgacgt tgtggcggcc ccggagcttc atcccggata ggtcgtaata
     5041 tgcgctcccg tattttatgc cgctcccgtc gtaggagacc ccgttcttca catcgcccgc
     5101 gccgaaagga attttgcccg cctttatggc cgggacgtat ccggcgatca ccgggtcgtc
     5161 ggccttatag atacaccagt catgctcagt ctggttcgag aagattttca tcttcgactc
     5221 cgcgtactct tcgaagctcg cgtggtggtc gaggtggttg ggggtgacgt tgagtatgac
     5281 ggagacgtcc gggcggaagg actctatacc ctgaagctgg aagctgctga gctcaagcac
     5341 gaggatatcg taacggtcgc agtcatcggc tatctgtatg aggggcgtac ctatgttggc
     5401 tccgaggaag actctcctgc cgttcttctc gagtatgcgc gcgatgagcg tggacgtggt
     5461 cgtctttccg ttcgagcccg taacggctat cgtcggtctg cgtatgaacc tggaggcgag
     5521 ctcgacctcg cttatcacct ctatgcaact ggctagcgcc tcgcggacgg gaggaatgcc
     5581 gaagggaact ccgggactca gcacaatcgt acctgacgaa aggaacgtct cgctcgtgtg
     5641 cccgcctgtc tcgcagctta cgccccagga ctcgagcgtc ttcacctgcc cggctatttc
     5701 cccggccggg gagctgtcgc ttacggacac ccgcgcccct ttcccgagca ggaatttaac
     5761 tgtctccata ccggtcttgc cgactccgac tacgagcact ttcctgtccc ttaactccat
     5821 gagattttta ttgccaccgc ctttaatgca tatcaaaaac cgtcacagct tttatctgag
     5881 cttgagcgtc gatagagcga acagagagag aacgacggat ataatccaga accttataac
     5941 gaccttcgac tcagaccagc ctttgagctc gaaatgatgg tgtagagggg ccatcctgaa
     6001 gaccctcttc ccggtgagct tgaatgaagc cacctgtatt atcaccgaca tagcctcggc
     6061 gacgaagacc ccgccaacga ggatgagaag tatctcctgc ttcagtatca gggcgaccga
     6121 tccgatcgca gcgccgagcg cgagggagcc tacgtctccc atgaagacct ccgcagggtg
     6181 agagttgtac cataagaagc cgagacacgc cccgccgacc gccgccagat agaccgcgag
     6241 ctcgccgccc tccgggatgt agggtatctg gaggtacttc gagaattccg cgtgcccgga
     6301 tagatacgcg aatatgacgt aggtcgccgc cgcgacggca atcgagccta tggcgagacc
     6361 gtcgagaccg tccgtaaggt tcaccccgtt cgaagcgcca acgaccacga tgagcgcaaa
     6421 cgggatgtag agccagccca ggtttatcac cgctttcttg ataaacggga acacgacgga
     6481 agtgaaggag tattccgcca cctggttcgc gctcagcgac atcgtgaagc catcgaggtc
     6541 gctcattacg agaagaacga aaagtattcc gaacaatatc tggagggaga actttaccct
     6601 cgccttcatg cccttctttt tcgagtattt gagccagtcg tcgaggaagc ctatgagcgc
     6661 gtagctcatc gtaaagagga gcaccgacca gactatctcg ctcgtgaggt tgagccatag
     6721 gagcgacgag atgagtatcg acagtattat aaatgccccg cccatcgtcg gtgtcccggc
     6781 cttggtcttg tgcgaagccg gtccgtcgtc ccttatgttc tcctggaatt gccttttcct
     6841 caggaaattg atgaagaatc cgcccagaat tatgcttatc aggaacgccg tcacgcccgc
     6901 gccgaacgag cggaacgtga tgtatctgaa aacgttgaaa agaatgaagt cgcccttcag
     6961 caaataaaga agatgaaaca tttaaatact ccctagacaa gcttctttgt tacctcttcc
     7021 atcctcatcc cccgcgagcc ctttacgagc acgagatcgc ccggcccggc aatctctctg
     7081 acaatattcg ccgcttcctc gtgcgattcc gcatagaagc agttgggtcc cccgcccgcc
     7141 ccttcgagga gagtccgtcc gaagttcccg catgcaatca cgtaatcgac gcccgacgcg
     7201 gacaggtatt cgccgacgcc gtagtgctcg cccgcgctct tttccccgag ctcgagcata
     7261 tcacccagga ctgctatcgc cctgcctccg ccctttagac ccattagctc gtccgcggcg
     7321 ctccgcatgg agtccggatt tgcattatat gtgtcgttga ttaccctgaa gcccgacggg
     7381 ctctccagga cctcgagcct catgtacccc ggagtaaaac cttcgaggcc cgcgattatt
     7441 tcgtcctttc cgcaccccat ggaaagcgcg ataccggaag cgcagagtgc gttcatcacg
     7501 ttgtgcacgc cgatgccttt aatgcggacg gggaattctt cgtttttcgt tcttattaaa
     7561 aaatcgatcc ccgagaagcc gtcctgaatt atatttcgtg cagttatatc cgctccgtcc
     7621 gattttatgc cgtacgttat ttttttgcag tccgtttttt tagcgatctc ggcagtccgc
     7681 gggtcgtccg cgtttacgac gaagacgttt tcgccggtga acccctcgac gagctctccc
     7741 ttcgctctcg caaccccttc tataccgccc agcttctcga gatgcgccct gcctatgttc
     7801 gtgatagcgc ccgtgtcggg gagagcaatc tccgcgagtc tcttaatctc tccgaaatcg
     7861 ttcatcccta actcgacgac cgccgcctta tagctcgcgt cgagtttgaa gagcgtaagg
     7921 ggaagaccga tcaggttatt gaaattgccc gtgttcttga gcgtcttgta cttccgcgag
     7981 accatgctcc aggccatctc cttcgtcgtg gtcttgccgt tagatcccgt taccgccgct
     8041 agcttcaatt gaggaaagct ctttctccac tgagaagcga ggtccccgag cgccctgagg
     8101 gtggaaggga cccggatcag taatttcccg gcttcatgag cgatggagtt ctgttcgata
     8161 accgcgccgc ccgcgccctt ttttatggcc tcgtccacgt actcgtggcc gtcgtagttc
     8221 tcgcccctga gggcgaagaa tatctccccc gaggatatgt tcctcgaatc ggtgcttacg
     8281 cccgagaaag agattccgtt cccgcctgaa acgagcttcc cgcttgtaga attgagaacg
     8341 aaatcaagtc cgagcatttt attatcgaaa aactaattaa gcatctcctt caatatttcc
     8401 agggccgcag tcctgtcatc gaaagggtat ttcgtcgtgc ctattatctg ataatcctcg
     8461 tgccccttgc ccgctatgag cacggtatcc ttctccgccg ccatgcatat cgcttttctg
     8521 atggcctcct tt
//
LOCUS       DASSJH010000061         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1421370, whole genome shotgun sequence.
ACCESSION   DASSJH010000061 DASSJH010000000
VERSION     DASSJH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421370"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1035
                     /gene="ispH"
                     /locus_tag="VFJ67_01345"
     CDS             85..1035
                     /gene="ispH"
                     /locus_tag="VFJ67_01345"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET7289054.1"
                     /translation="MRVIVAKPRGFCAGVDRAIEIVERALDMYGPPIYVRHAIVHNKR
                     VVESLKSKGVRFVEELSEIDKKGAKVIFSAHGISPAVRDEAESMGLEVVDAVCPLVTK
                     VHNEVRHYADIGYTIILIGHRNHVEVIGTSGEAPERVVVVESVAEAEEVVLEDPDRVA
                     YVTQTTLSVDDTKDILEVLKRRFPKILSPSKLDICYATQNRQDAVKALAAQSDIIFIM
                     GSPESSNSNRLVEVAKSSGVKNAYLIEGADNLKREMFRNGMVVGLSSGASTPETVVLE
                     VITRLREFGADEVEELDGKEEHTRFPFPDTIQFKTASETH"
     gene            complement(1092..1601)
                     /locus_tag="VFJ67_01350"
     CDS             complement(1092..1601)
                     /locus_tag="VFJ67_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7289055.1"
                     /translation="MSDNIRRILSDNAEYAKEFGDKGKLGIIPARKIAILTCMDARIE
                     PMRLAGLKTGDAHIIRNAGGRASDDAIRSLIVSNRLVGTNEWLVIHHTGCGIENMTNE
                     KMAGIAKASFESGEGDYINWLTIGDRIESIRDDVKRLREHPLTPSDVTVTGFMYDIET
                     GLLNEVSCD"
     gene            complement(1685..>2697)
                     /locus_tag="VFJ67_01355"
     CDS             complement(1685..>2697)
                     /locus_tag="VFJ67_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET7289056.1"
                     /translation="EEEPEEKEPGTKSFKYGRDVTDPNFRLLYAYFKELNDEKLLSRR
                     DEAVLAAKIKLCENGAKKIEVRSKNKQARGTVRPAKDGDILDALSRSYSSRSRQFRDK
                     FIKSNLRLVIELANKYTGRGLPLTDLIQEGNIGLMKAVTKFDHSKGFKFSTYASWWIH
                     QALSRAVMEQSHVILIPVYLQELAAKVFRAKAKIEHNSDKNALPEQIVSETNIPLDAV
                     VTILKGNDMVVPLELLPGKDDSKSYLDITSDPKSKRPEYYLTLQSINEEVRDSLGLLS
                     ERERDVLMMRFGIDYENDHKLEEIADKYGLSRERIRQIEKDALTKLAGSARSGVLKEF
                     LN"
BASE COUNT          551 a          720 c          724 g          702 t
ORIGIN      
        1 gctcttattg aagtcattga agatatgcct cttcgactgt aaaggacccg actcatcgag
       61 tattattaac gtgggagtag atatatgcgt gtcattgtcg ccaagccgag gggtttttgc
      121 gccggggtcg accgggccat agagattgtc gagcgcgcgc tcgatatgta cggcccgcca
      181 atatacgtcc gccacgcgat cgtccacaac aagcgggtcg tcgagtcgct caaaagcaaa
      241 ggggtcaggt tcgtggaaga gctctccgag atagacaaga agggcgcgaa ggtgatcttc
      301 agcgcccacg gtatcagccc cgcggttcgg gacgaagcgg agagtatggg gctcgaggtc
      361 gtcgatgcgg tctgtccgct cgtgactaaa gtccacaacg aagtgaggca ttacgccgat
      421 atcgggtata cgataatcct catcgggcac aggaaccacg tcgaggtcat agggacgagc
      481 ggcgaggctc ccgagagggt cgtggtggtg gaatcggtcg ctgaagccga ggaggtcgtg
      541 ctcgaagacc ccgacagggt cgcctatgtt actcagacca cgctcagtgt cgacgacacg
      601 aaagatatac tggaagtcct caagagaagg ttccccaaaa tattgagccc ctccaagctc
      661 gacatatgct acgccactca gaacaggcag gatgcggtca aggcgcttgc cgcgcagtcg
      721 gatattattt ttatcatggg ttcgcccgag agctctaatt cgaaccgcct cgtcgaggtc
      781 gccaaatcga gcggtgtgaa gaacgcctat cttatcgaag gtgcggataa tctgaagaga
      841 gagatgttcc ggaacggaat ggtcgtgggg ctgagctcgg gggcctcgac tcccgagact
      901 gtggttctcg aagtaataac ccgtctcagg gaattcgggg ccgatgaagt cgaagagctc
      961 gacggcaagg aggagcacac gaggttcccg ttcccggata cgatacagtt caagactgcg
     1021 tccgaaacac attaggccgc ttgctccggc tcaattgcag agcttatcct gatgttgcgc
     1081 gggattcggg ttcaatcgca ggacacttcg ttcagaagcc ccgtttctat atcgtacata
     1141 aaacctgtaa cggttacgtc ggaaggcgta agaggatgct ccctcagcct ttttacgtcg
     1201 tcccttatgc tttcgatcct gtcccctata gtgagccagt tgatataatc accttcgccg
     1261 gattcgaacg aagccttcgc tatacccgcc atcttttcgt ttgtcatgtt ttcgatcccg
     1321 catccggtat ggtgaatgac cagccattcg ttcgtgccca caagtctgtt ggaaacgata
     1381 agcgacctga tcgcgtcgtc gctcgctctc ccgcctgcgt ttctgattat gtgagcgtcc
     1441 cccgttttga ggcccgcaag cctcatgggc tctatacgcg cgtccataca cgtcaggatg
     1501 gcgatcttcc ttgcggggat tatccccagc tttcccttat caccgaattc ctttgcgtat
     1561 tccgcgttat cgcttaatat cctcctgatg ttgtcgctca tagtgcctcc cttatatatt
     1621 taggatgtta cgtgtcccgg gttatcatgc aatttggata ttaaaggttg taaagcaggt
     1681 gtgtttagtt caggaattcc tttaaaacgc cgctccgcgc cgagccagcc agcttcgtga
     1741 gagcgtcctt ctctatctgt cttatccttt ccctgctgag gccgtacttg tccgcgatct
     1801 cttcgagctt gtgatcgttt tcataatcga tgccgaacct catcatgagc acgtctcttt
     1861 ccctttcgga cagcagtccc agcgagtccc tgacctcttc gttgatcgac tggagggtca
     1921 gataatattc cggcctcttt gacttggggt cggaggttat atcgaggtat gacttggagt
     1981 cgtccttgcc ggggagaagc tcgagcggta ccaccatgtc gttccctttg agtatagtga
     2041 ccacggcgtc gagcggtatg ttcgtctcgc ttacgatctg ctctggtagc gcgttcttgt
     2101 ctgaattatg ctctatcttc gctttcgctc tgaaaacctt ggccgcgagc tcctggagat
     2161 aaacgggtat gagtattacg tgggactgct ccattacggc tctcgacaga gcctgatgaa
     2221 tccaccacga tgcgtatgtg gagaacttga accccttcga atggtcgaat ttcgtgacgg
     2281 ctttcatgag cccgatgttg ccttcctgaa taaggtctgt caggggcagt cctctgcccg
     2341 tgtatttatt ggcgagctct atcacgagcc tgaggttcga tttaatgaac ttgtcccgga
     2401 actgtcttga cctcgaagaa taagacctgg agagcgcgtc caaaatatcg ccgtctttgg
     2461 cgggcctgac cgtcccgcgc gcttgtttgt tcttcgagcg cacctctatt tttttcgccc
     2521 cgttttcgca tagctttatc ttcgcggcga gaaccgcctc gtcgcgtctg ctcaggagtt
     2581 tctcgtcgtt gagctctttg aaataagcgt agagaagcct gaagttcggg tcagtgacat
     2641 ccctcccgta tttgaatgac ttggtcccgg gctccttctc ttccggctct tcttcct
//
LOCUS       DASSJH010000062         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_879271, whole genome shotgun sequence.
ACCESSION   DASSJH010000062 DASSJH010000000
VERSION     DASSJH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879271"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..731
                     /locus_tag="VFJ67_01360"
     CDS             192..731
                     /locus_tag="VFJ67_01360"
                     /inference="COORDINATES: protein motif:HMM:NF037752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7289057.1"
                     /translation="MIKRIFGLFLLISVLYGLSALSARAGFLPEIPEGDCAFALVKIT
                     PQTGSNSQLFDFIQTVNGVDTEITVASNPLGPPDVLFVSQGTVVTYTEVPQEGWTLYD
                     IQCFDFTGVDFTTTETSATLECVNPGEFSLGFCAFYNRISADKIPTLSEWGMIAAAAG
                     LGLVGVFFAVRRKRLQASE"
     gene            1185..1703
                     /locus_tag="VFJ67_01365"
     CDS             1185..1703
                     /locus_tag="VFJ67_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289058.1"
                     /translation="MESNKSVKKFVLAAVFGVCAVAASASGAFAQPVYPQTPPPAPPP
                     ASPCSSQEGISCSEKCLPMVVNVETVKASGVAVSEVVESQQISMLAGKALDFRATCPA
                     GSFAIGGGYTLVPEDSTIDMKEIRISSSIADNMNPSGWNGWKVSVFRKEGAGSPDNCL
                     KVEVRAYCIQGQ"
     gene            complement(2578..>3322)
                     /locus_tag="VFJ67_01370"
     CDS             complement(2578..>3322)
                     /locus_tag="VFJ67_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7289059.1"
                     /translation="EWVPAPSIIAAGMLVFLSYEGFELIANVSDQVRDRERNLPLAYY
                     GSVATALVFYVLIIFVVLGHMSFESITASRDYTLSAAAEIFMGRPGFVLLAVGAILAT
                     ASAINAGLFGASKLPQMLARVEEAPPVYLRQYRGRRPVGLVVISVLVLLIVNFLDLHS
                     LSAAASAGFIVIFFMVNAANAKLARETGSSRRVSVTAAAVCLIALAIMLAETALSPEN
                     RKDLWFIAGLIAFPVLYQAVYRAKNIFFL"
BASE COUNT          814 a          761 c          946 g          801 t
ORIGIN      
        1 tgtctgagct gccgctccgg atgatgagag gacgagcatg acggctgcga ggagggctgt
       61 ccgcatcccg aagattatac atgcccggcc ccggcattgg gaatccggcg ggcggggcgg
      121 gtctgctatc agcacttgca aaaaagttaa ataaatcgta caataattta tctcgtttaa
      181 ggaggacata tatgataaaa cgtatattcg gtttatttct cttaatatcc gttttatatg
      241 gtttatcagc tttatcagcg cgcgccggtt ttcttccgga aatacctgag ggggattgtg
      301 cgtttgcctt ggtcaaaatt acgccccaga ccggatcaaa ttcacagttg tttgatttta
      361 ttcagactgt gaacggcgtc gataccgaga taaccgttgc atcgaatccg ctaggtccgc
      421 ctgatgtgtt attcgtaagc cagggcaccg tagtgaccta tacggaggtt ccgcaggaag
      481 ggtggacgct ctatgatatt caatgctttg attttacagg cgtcgatttt acgacgaccg
      541 agaccagcgc cacgctcgaa tgcgtgaatc cgggtgaatt ttctctcggt ttctgcgctt
      601 tctacaacag gatttccgcg gataagattc cgacgctgag cgagtgggga atgatcgcgg
      661 cggctgcggg gttgggattg gtcggcgtgt tcttcgctgt aaggcgcaaa cggttacaag
      721 cgagcgagta accggacgcc ctacgctgat tgcggaagag ttatttagac accgcatgtg
      781 ggagcataaa ccaaagtaga cgcaggctgg tttgtaaggg cgtaagcgtt aaatataaat
      841 agaacgtaaa gatttaaagg actggaaagc cccttttctc cgcatgagcg gtgtgaaggg
      901 gctttttgtt ttaatgatgg atgatagaag gcgattgttt gattgttagg aataataagg
      961 tacaccctct ccctagccct ctcccctcct atgaagggag aggggattgg tcgaaacaca
     1021 aatcggttag gcttcgtttg ctagttgtgc ggaagaggcg gattgaatcg taaaatcatg
     1081 ctatctgtgc gattatgcgc ggcatgttga atcgcaattg ataatatagt aatatcgtgt
     1141 gagcaagggg agttataaaa tccattcaag ggaggaatta gattatggag agtaacaaat
     1201 ctgtgaagaa gtttgttctt gccgccgtct tcggcgtgtg cgccgtcgcc gcatcagcgt
     1261 ctggcgcttt cgcgcagccc gtttatccgc agacgcctcc tccggctccg ccgcctgcgt
     1321 cgccgtgctc gtcgcaggaa ggtatatcct gctcggaaaa gtgcctgccg atggtggtca
     1381 acgtggagac ggtcaaggct agcggcgtcg ccgtatcgga ggtcgtggaa agccagcaga
     1441 taagcatgct cgcggggaag gcgctcgatt tcagggcgac gtgcccggcg ggttctttcg
     1501 caatcggcgg gggatacacg ctcgtgcccg aagactcgac gatcgatatg aaggagatcc
     1561 gcatttcttc gagcattgcc gacaatatga acccgagcgg atggaacgga tggaaggtgt
     1621 cggtgttcag gaaagaaggc gcgggttcgc ccgacaactg cctcaaggtc gaggtgcgcg
     1681 cgtactgcat acaggggcag taaagagcgc ccgaaccggc gcggggtgaa tgtcggtgcc
     1741 gcgtcgattc aaatacaata tttggaaata agtgctcccc gcccggccgg aaagggtcgg
     1801 acggggagat tttttttatg ggtaaatacg ggaaaagata agaaggaaca aaggcaattg
     1861 tttttatcct tcttttcctt gatgaaaaga agcaaaaatc atccgactaa cagaatttac
     1921 taaaaatatt gtgtttaagc taaaatcttt caaatgggaa gctgaaagat ttttttacgc
     1981 ttaaacaccc gattttctta acgccaattc tgtaatgtcg ggaatgatag agctataaat
     2041 tgccaactcg ccatgttaat gaggttgggt acaaggtgag caagagcgac caattgtttt
     2101 ggcttaaagg gagcctgttc tgaacatttg cgttcgcgta ttcaaccgga atagaccgga
     2161 ttctcactag ttcgaatccg ttacttgttc gcaattgttc ttcgactagc tcaggacgaa
     2221 cgggtaaaaa ctaaatcctc acccttaacc cttccccctc ctttaaaggg ggagggaaat
     2281 tgaggaagaa aatattcgtg cgatatttga tttagattcc cttctgctca gctttttgtg
     2341 cgcggatgta tcttatgcct ctgttaacca ctaggtcggg agttcgaatc tctccgccgg
     2401 agccaattat tgcagtaggt tacagcagat tttcagttac cctgtttttc gttgctacag
     2461 aaattgctac agttctatct tctttttcgg gaaacgaaga gaaataaaag gaaacaaaat
     2521 actgtatatt atataatcat gattgtaggt tatgacacaa tgagaaaaag tgtgaaacta
     2581 taggaaaaat atatttttag ctctgtaaac cgcctgatac agcacgggga aggcgatgag
     2641 gcccgctatg aaccagaggt ctttcctgtt ctccgggctt agggccgtct ccgcgagcat
     2701 gatggcgaga gcgatgaggc acacggcggc ggccgttacg gacacgcgcc tgctcgaacc
     2761 cgtctcccgc gcgagcttcg cgttggccgc gttgaccatg aagaatatga cgatgaaccc
     2821 ggcgctcgcg gcggccgaga gggagtggag atcgaggaag ttcactatca gcagcacgag
     2881 aaccgagatc acgacgaggc ccacgggacg tcggcccctg tattgacgga ggtacaccgg
     2941 aggggcttcc tcgaccctcg cgagcatctg cgggagcttc gacgcgccga agagccccgc
     3001 gtttatggcg gaggccgtgg cgagtatggc gcctacggcg aggagcacga agcccggcct
     3061 gcccatgaag atctcggccg cggcggagag ggtgtagtcg cgggaagccg ttatggattc
     3121 gaagctcatg tgcccgagca cgacgaaaat aatcagcacg tagaatacga gcgcggtcgc
     3181 gaccgagccg tagtaggcga gcgggaggtt cctctcgcgg tcccttacct ggtctgagac
     3241 attcgctatc aattcgaacc cctcgtagct caggaatacg agcatgcccg cagctattat
     3301 gctcggggcc gggacccatt cg
//
LOCUS       DASSJH010000063         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_340382, whole genome shotgun sequence.
ACCESSION   DASSJH010000063 DASSJH010000000
VERSION     DASSJH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340382"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFJ67_01375"
     CDS             <1..236
                     /locus_tag="VFJ67_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289060.1"
                     /translation="SYESGDKAGALSKLKSAYDSISNLNRSAYGNAKTAQQEAELRDA
                     LENMSSPAAPAPASDQGKALIKEQKASAREAQK"
     gene            325..987
                     /locus_tag="VFJ67_01380"
     CDS             325..987
                     /locus_tag="VFJ67_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289061.1"
                     /translation="MKSLLRSFIVPVSLSGGWGVMTYLLGYIFLSRVPIGYMFFYPII
                     MTGVVVVAAVSGGSRVSHARAALTGFITGFIYLLVSPMFPLLASVLAGASLGGGLSEG
                     RGRFGGLLDGAVSTLKGMIIFPIVIYTGEILGACVYIFLNSMFLGMVFWAAWLGLGVC
                     IIRFPVTGRRGDSGNPRRVRGLDEFRDEASEIKRDLGELGTGIDNTLSEGAPMQPSGL
                     NF"
     gene            1104..1823
                     /locus_tag="VFJ67_01385"
     CDS             1104..1823
                     /locus_tag="VFJ67_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289062.1"
                     /translation="MIHFRRVFIISLTALLFYVPGLMSRAVETRTESEAVSPSELENS
                     EEAAETEVEDIEPEPQRLYCAEQWTKLYEGQKAGIKVTTKGHYNQIAMFSCPDCSLDE
                     HYVKPFLESEYRGKTGLMRLHECGFTQAIFKGMRGTEAIVVDVPSVFPDPNRLVCIND
                     WSEDYRKNYPSIQISSRGELNEAIVFSCLNCPFQKSFIAPFLLTVTDGKTAMERMKEC
                     GFTEVVFTNPMGTREVVRKVR"
     gene            1913..2122
                     /locus_tag="VFJ67_01390"
     CDS             1913..2122
                     /locus_tag="VFJ67_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289063.1"
                     /translation="MGEVGTFPFGWVRGEGGDNWQIIWDPKTKTVFAEGALSKKVLTL
                     GESPTWEDAKRFADGVISDPRGYFS"
     gene            2277..3176
                     /locus_tag="VFJ67_01395"
     CDS             2277..3176
                     /locus_tag="VFJ67_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7289064.1"
                     /translation="MKFMEQELVSIPSGGGKVDGLYYHSTVKDDRIPEKDVGIVHVHG
                     FLGNFLDGSQRFLPPILAEAGYSSLAINTRMANFGLFFGYGIIDDVIPQIDSAVDYLL
                     KLGYKKIIISGYSLGTCMVLRYASLRSDPDRYPYIKGVMSLATPYSMPESIRRRWDRW
                     RSEPSYEEVYEESKEILKPDPYNTQHDRTILIYKARGNTYLPWHTEIYTYKTWWFLAG
                     PEAYMAKAYLKMERIKLPLLLIQGWYDDVVRPEETHKLADLALQSGNTDVSAFYVNAG
                     HTFEGKEEELGEIIVRWLDKRFR"
     gene            3195..4109
                     /locus_tag="VFJ67_01400"
     CDS             3195..4109
                     /locus_tag="VFJ67_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7289065.1"
                     /translation="MVPRQRKLLAFSAPDGFLINAVLVSGEFRKEKDLYNTPIVLLVH
                     GVLGHFLARGTPRLLPNALVERGISSLSINTRLAFTGQIFGGGIFDESVLDIDAAIDM
                     LVKEGFKKIFVLGWSLGANITVYYAARGAHKNVKGLILEGCSTSLPLSHKRRLDKWQS
                     IPSYEHIYEIAKKVLKPDPSETKNDRIFVVYRAWGPSFDPSDCEMFSYRTWWYMRSPE
                     AQNAKTNEIISGVKVPVLFIHGAEDDVVGADEVRELLGILKKSGNRKAELTFIPGAKH
                     DCMENPSFTVDTVVEWINKSVAGRKKKG"
BASE COUNT         1038 a         1119 c         1250 g          862 t
ORIGIN      
        1 cctcctacga atcgggtgac aaggcgggcg cactgtccaa gctgaagagc gcttacgaca
       61 gcatatccaa tctcaacaga agcgcctacg ggaatgcgaa gaccgcgcag caggaggccg
      121 agctaaggga cgcgctcgag aatatgtcct cacccgctgc cccggcgccc gcttccgacc
      181 aggggaaggc tctcataaaa gaacagaaag cgagcgcgag agaggctcag aagtaacaac
      241 cgccggtttc gcgtaaatga agaggggagc tgcttccggg cggctccccg ttcgtctcaa
      301 aatcagaaaa caacggagac accaatgaaa tccttgctaa gatcattcat cgtacctgta
      361 tcgctctcgg gagggtgggg cgtgatgacg tacctcctgg gttatatatt cctgagcaga
      421 gtgcctatcg ggtatatgtt cttctatccc ataatcatga cgggcgtggt cgtcgtagcc
      481 gccgtctcgg gcgggagccg cgtctcgcac gcgagggccg cgctcacggg tttcatcacc
      541 ggcttcatat acctccttgt ctcgccgatg ttcccgctcc tcgcgtcggt gctcgcaggc
      601 gcgagcctcg gcgggggact atcagaggga aggggcaggt tcggcgggct cctcgacggc
      661 gcggtctcga ccctgaaagg gatgataatt ttccctatag ttatttatac cggcgaaata
      721 ttaggagcat gtgtttacat attcctcaac tctatgttct tgggcatggt gttttgggcg
      781 gcgtggctag gcctcggtgt gtgcatcata cggtttcccg tgaccgggag gagaggcgac
      841 agcgggaatc cgcgccgtgt acggggtctg gatgagttca gggacgaggc ttccgaaata
      901 aaaagagacc tcggggagtt gggtacgggc atagataaca cgctttccga aggggctcct
      961 atgcagccgt ccggactcaa tttctgatca ggcatctccc aatgtccccc gatggtttta
     1021 aggcgaaatt aatgtgttac tcaaggtatt ccatatatcc tccgaatgct gtaaaataaa
     1081 aggtgaaaga ggaggatgac cggatgatac atttccgccg cgtattcata ataagcctta
     1141 cggcattgct tttttacgtc cccggcttga tgtcccgcgc tgtcgagacg cgtaccgaat
     1201 ccgaagccgt gagtccctcg gagctggaga actctgagga ggcggccgaa acggaggtcg
     1261 aggatatcga gcccgagccc cagcgccttt actgcgccga gcagtggacg aagctctacg
     1321 agggacagaa ggccggcata aaggtcacga cgaaggggca ttataaccag atcgccatgt
     1381 tcagctgccc tgactgcagc ctcgacgagc attacgtgaa gccgttcctc gaatccgaat
     1441 acaggggaaa gactggtctc atgagacttc acgaatgcgg attcacgcag gcgatattca
     1501 aggggatgag gggtacggag gcgatagtcg tcgacgtgcc gagcgtattc cccgacccga
     1561 acaggctcgt ctgcataaac gactggagcg aggactaccg gaagaactat ccgagcatac
     1621 agatatcgtc gaggggcgag ctgaacgaag cgatagtctt ctcatgcctc aactgcccgt
     1681 tccagaaatc gttcatcgcg cctttcctcc tgaccgtcac cgacgggaag acggccatgg
     1741 agcgcatgaa ggaatgcggc ttcaccgagg tcgtgttcac gaacccgatg ggcacgaggg
     1801 aagtcgtccg gaaagtgcgt taggcctcgc ttgacaaaaa accggattgg aatattttac
     1861 ctcttgcgcc gggcgtgcac gggcgcggat cattaattaa gggagctttc aagtgggcga
     1921 ggtcggaacg ttccccttcg gctgggtgag gggcgagggt ggtgacaact ggcagataat
     1981 atgggacccg aagacgaaga cggtcttcgc cgagggagct ctctcgaaaa aggtgcttac
     2041 gcttggggaa tccccgacct gggaggacgc caagcgcttc gccgacgggg tgatcagcga
     2101 cccgcggggt tacttttcct gagctgtatg agttccatca gtcttaatat tttctgatta
     2161 acacctcagc tattctccta actcaatctc tgctaaaata gagcctgaca attcttcagc
     2221 ataaggtaac ttgcggagaa atcatatatg agcatgtatt gcgtgcggat gaagatatga
     2281 aattcatgga gcaggagctc gtttcgatac cctcgggcgg gggcaaggtg gacggcctct
     2341 actaccacag cacggtaaag gacgaccgta taccggagaa agacgtaggc atagtccacg
     2401 tccacgggtt cctcgggaac ttcctcgacg ggagccagcg cttcctcccc ccaatactcg
     2461 ccgaggccgg gtattcctcc ctcgcgatca acacgcgcat ggccaacttc ggcctcttct
     2521 tcggatacgg catcatcgac gacgtgatcc cgcagataga cagcgccgtc gattacctcc
     2581 tcaagctcgg ttacaaaaaa ataatcatat ccggttacag cctcggaacg tgcatggtgc
     2641 tccggtacgc ctctctcagg agcgatcccg accgctaccc ttacattaaa ggcgtcatgt
     2701 cgctcgcgac cccctattcg atgcctgaat ccataaggag gcggtgggac aggtggagga
     2761 gcgagccatc ttatgaagag gtgtacgagg agtcgaagga gatactcaag cctgatccct
     2821 acaacaccca gcacgaccgc acgatactca tatacaaggc gcgcgggaat acgtacctcc
     2881 cgtggcatac ggagatctac acgtacaaga cgtggtggtt cctggccggg cccgaggcct
     2941 atatggcgaa ggcgtatctc aagatggaga ggataaagct gcctctttta ctcatacagg
     3001 ggtggtacga cgacgtcgtc aggcccgaag agacccacaa gctcgccgac ctggcattac
     3061 agtccgggaa cacggacgtg tccgcctttt acgtgaacgc cgggcacacg ttcgagggta
     3121 aggaagagga gctcggggag atcatcgtca gatggctcga caagaggttc aggtaacaga
     3181 agggacggtt ataaatggtt ccgagacaga gaaagctctt agcattcagc gcgcccgacg
     3241 ggttcctcat aaacgcagta ctcgtgtcgg gcgaattcag gaaggaaaaa gacctctata
     3301 acacgccgat agtgctcctc gtacacggcg tgctcggaca cttcctcgcg agggggacgc
     3361 cgaggctcct gccgaacgcg ctcgtcgagc gggggataag ctcgctttcg atcaacacta
     3421 gactcgcgtt cacgggccag atattcgggg gaggcatatt cgacgaatcg gtgctcgaca
     3481 tagacgccgc catcgacatg ctggtaaagg agggattcaa gaagatattc gttctcggct
     3541 ggagcctcgg ggcgaacatc accgtatatt acgcggcccg cggcgctcac aagaacgtga
     3601 agggcctcat actcgaaggc tgctcgacct cgctcccgct ctcccacaag cggaggctcg
     3661 acaagtggca gagcatcccg tcatacgagc acatttacga gatagcaaag aaggtgctca
     3721 agcccgaccc ttcggagacg aagaacgacc gcatattcgt tgtgtacagg gcgtgggggc
     3781 cttcgttcga tccttccgac tgcgagatgt tctcgtacag gacttggtgg tacatgagga
     3841 gccccgaggc gcagaacgcg aagacgaacg agataatctc cggcgtgaag gtgccggtgc
     3901 tgttcataca cggggccgag gacgacgtgg tcggggccga cgaggtgcgg gagctcctcg
     3961 ggatactcaa gaagtcaggg aacaggaagg ccgagctcac gttcattccc ggtgcgaagc
     4021 acgactgcat ggagaaccct tcgttcacgg ttgatacggt agtcgagtgg attaataagt
     4081 ctgttgcggg gaggaagaag aaaggttgaa aggagagtaa accttgtcta ctttgctgaa
     4141 acgcatttaa tacgaattat gaaaggacta aagaaaggag aaaagcaatt tatttatcct
     4201 tcttttcctt gatgaaaaga agcaaaaatc aaccgacaga acataatttg ctaaaaatct
     4261 cgcattaag
//
LOCUS       DASSJH010000064         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_232708, whole genome shotgun sequence.
ACCESSION   DASSJH010000064 DASSJH010000000
VERSION     DASSJH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232708"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFJ67_01405"
     CDS             complement(<1..400)
                     /locus_tag="VFJ67_01405"
                     /inference="COORDINATES: protein motif:HMM:NF028349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289066.1"
                     /translation="MNRKIILFYNSMWHDPLVYPENELPEGYVITMDRSLMDEAAAVV
                     FHLPTLPPEKFTGGGVEKKKGQLWVAWYLECEVNYPYMADPDFMSRFDLRMSHRLNAD
                     IFSPYFPPDFAELSRKPVPEKEPGKIVNAFI"
     gene            1047..1120
                     /locus_tag="VFJ67_01410"
     tRNA            1047..1120
                     /locus_tag="VFJ67_01410"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1079..1081,aa:Gln,seq:ttg)
     gene            1191..>2838
                     /locus_tag="VFJ67_01415"
     CDS             1191..>2838
                     /locus_tag="VFJ67_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7289067.1"
                     /translation="MNPSQAEAVTHAGGPVLVLAGAGSGKTRIITERIAHLIEKKGEN
                     PSGILAVTFTNKAANEMKERLRRLIGPAVKSLWLGTFHSTCLRILKREIKHLGGFRQD
                     FVIYDDGDQLKLIKQCMERLSIGEKELDPKSVRSKIDRAKNRGLDPDEAGRDAYDERI
                     TKIYGLYEEELRKLNALDFGDLLYLTVRLFSEKPEVLRQYQETFRHVLVDEYQDTNHV
                     QYRIVRLLSGKHRNIFVVGDDNQSIYGWRGADITNILNFERDFPDSSVIKLEKNYRST
                     KTILGAANGLILKNRNRHEKNLWTDNPEGEKIQYYVAHDDRDEARYVAAEIESIVRSR
                     ACSYKNAAVLYRTNNQSRIIEEELVRKGIPYAIVGGIGFYQRAEIKDIMAYLRVIANP
                     LDDLSLKRIVNVPPRGIGKGTVERLEELAKKEGISLMDAMRKAPGENLLPARAAMNLG
                     RFLEKMERLIGYGEKYERGELLDLVLQETGYLDLLEREEEKRENVGEILNLAAEFEKE
                     SEKAWLRDFLDTVTLSSDVDSYDEKDDRVALMTLHSAKGLE"
BASE COUNT          768 a          718 c          794 g          558 t
ORIGIN      
        1 agatgaaggc gttcacgatt ttccccggct ccttttccgg gacaggcttg cgcgaaagct
       61 cggcgaagtc aggcgggaaa tacgggctga aaatatccgc attcaggcgg tgggacattc
      121 gcagatcgaa gcggctcata aaatcgggat cggccatgta tggataattt acttcgcact
      181 ccaaatacca ggcgacccac aactgcccct tctttttttc gactccgccg cccgtaaact
      241 tttctggcgg gagcgtaggc agatggaata cgactgcggc ggcttcgtcc atgagactcc
      301 tgtccatggt aatgacgtaa ccctccggca gctcattctc cggataaacg aggggatcgt
      361 gccacataga attgtaaaag agtattattt tccggttcat ttattccgag ccgcgctttt
      421 agccgttatt ctatgatatt cggttttcca tgtccacgga ccctcgcccg ggatggagga
      481 ctgaagtcga aatgcgagat tgccgcgcgg aacctgtcct gagcgtggtc gaaggattcg
      541 gaacacataa taaaggcaaa atatgttttc ttcttttcct tgatgaaaag aagcaaaaat
      601 caaccgacag aacagaattt gctaaaaatc ttgcgtttaa tctaaaatct ttcaatagaa
      661 agctgaaaga tttttttact cttaaacacg cgattttctt aacgcaaatt ctgtaatgtc
      721 gggaaagata aatcccctct gcattacatg ttatccagga gaattccagc cccaaattat
      781 ttaacatcta cagcaatgac agataaagta attctcctgt ccggctgccg acttcgctat
      841 gctacgcact tcgacacgct cagtgcgaac ggattacatg aaatttatga atcgaaggta
      901 tcacccccct cctaaccttc ccccctctag ggggaaggaa ttaatattaa ggatgaacgg
      961 gcggtgctga acaaagtctg tgccccagac agactcgatt tcattgatat aacaatcaag
     1021 tcttggtaac attaacgggt agcagttggg gcgtcgtcta acggtaggac tcaggccttt
     1081 ggagcctgct gtggtggttc gagtccaccc gccccagcca gtgaagcgga attgttccat
     1141 atttcattct cacacatcga actcatatga catccgaaaa taaagacaat ttgaacccgt
     1201 cccaagcgga agccgtgacc cacgcgggcg ggcccgtgct cgtgctggcg ggcgcaggct
     1261 cggggaagac gaggataatc acggagagga tcgctcacct catagagaag aaaggcgaga
     1321 acccgtccgg catacttgcc gtcacattca cgaacaaagc ggcgaacgag atgaaggaaa
     1381 ggctccgccg ccttatcggg cccgccgtga aaagcctctg gctcgggaca ttccactcga
     1441 catgtctgag gatactgaag cgggagataa aacatctcgg cgggttcagg caggacttcg
     1501 tcatttacga cgacggcgac cagctgaagc tgatcaagca atgcatggaa aggctaagca
     1561 tcggcgagaa ggagctcgac ccgaagtcgg tgaggtcgaa gatagacagg gcaaagaaca
     1621 gagggctcga ccccgacgag gcggggagag atgcctacga cgagaggatc acgaaaatct
     1681 acggccttta cgaagaggag ctcaggaagc tgaacgcgct cgatttcggc gacctgctct
     1741 atttaaccgt aaggctcttc agcgagaaac cggaggtcct ccgccaatac caggagacgt
     1801 tccgccacgt gctcgtggac gagtaccagg atacgaacca cgtgcagtac agaatcgtcc
     1861 ggctgctgtc ggggaagcac aggaacatat tcgtcgtcgg ggacgacaac cagtctatct
     1921 acggctggcg gggggcggat ataacgaaca tactcaactt cgagagggac ttccccgatt
     1981 caagcgttat caagctcgaa aagaattaca ggtccactaa aactatacta ggcgccgcga
     2041 acgggctcat actcaagaac cggaacaggc atgagaagaa cctctggacc gataatccgg
     2101 aaggagagaa gatacagtac tacgtcgctc acgacgacag ggacgaggca aggtacgtgg
     2161 ccgcggagat cgagtccatc gtacggagcc gggcgtgctc gtacaagaac gccgcggtct
     2221 tatacaggac aaacaaccag tcgaggataa tagaggaaga gctcgtaagg aaaggcatcc
     2281 cctacgctat agtggggggc ataggttttt accagcgggc cgagataaag gacatcatgg
     2341 cttatctcag ggtgatcgcg aacccgctcg atgatctgag cctcaagcgc attgtcaacg
     2401 tgcccccgcg cgggatcggg aagggaacgg tcgaaaggct cgaagagctc gcgaagaagg
     2461 aggggatatc cctcatggac gcaatgcgca aggcgcccgg agagaacctg ctgccggcga
     2521 gagccgcgat gaacctcggg agattcctcg aaaagatgga acggctgatc ggatacgggg
     2581 agaaatacga gagaggggag ctgctcgacc tcgtgctcca ggagaccgga taccttgacc
     2641 tgctcgaaag ggaggaggag aagagggaga acgtgggcga gatactgaac ctggccgccg
     2701 agttcgagaa ggaatccgag aaggcatggc tccgggactt cctcgacact gtcacgctct
     2761 cgagcgacgt cgacagctac gacgagaagg acgacagggt cgcgctcatg acgctccact
     2821 cggcaaaggg tctcgagt
//
LOCUS       DASSJH010000065         4407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1205218, whole genome shotgun sequence.
ACCESSION   DASSJH010000065 DASSJH010000000
VERSION     DASSJH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205218"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2582
                     /gene="aceE"
                     /locus_tag="VFJ67_01420"
     CDS             <1..2582
                     /gene="aceE"
                     /locus_tag="VFJ67_01420"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET7289068.1"
                     /translation="ERVSKLLKILQIHAQKKGVRLQFTANTPYINTFPVEMQPPYPGN
                     QAIEWNIRSFIRWNAMAMVVRANRLEEGIGGHISTFASAAALYEVAFNHFLRARNGNK
                     EGDIVYFQGHAAPGIYARAFLEGRLTIENLQNFRRELQPGGGLPSYPHPWLMPGFWQF
                     PTVSMGLSPIMSIYQARFSRYLEDRGLKEHSDSKVWAFLGDGECDEPEALGAITLAAR
                     EKLDNLIFVVNCNLQRLDGPVRGNGKIIQELEAIFRGAGWNVIKVIWGSNWDPLLAKD
                     KDGVLVKRMGEVVDGEYQKYTVESGDYIRKHFFGTDPRLLDMVKDLTDEELQKLRRGG
                     HDPEKIYPAYKAAVENRGSPTVILAKTVKGYGLGEAGEGRNITHQQKKLNEDELKEFR
                     TRFDIPISDEDVVRAPFYRPHDDSPEIKYIQERRLELGGYLPKRVVDCQPLEQPSPSI
                     FEEFYKGSGERPVATTMAIVRMLSKLLKDKNIGKYIVPIVPDEARTFGMESLFRQVGI
                     YSHVGQLYEPVDAETLLFYKEARDGQILEEGITEAGSMSSFIAAGTSYATYGINMIPF
                     FIFYSMFGFQRIGDLVWAAGDSRAKGFMVGGTSGRTTLAGEGLQHQDGHSHLLAYPVP
                     NLKAYDPAFAYEIAVIIEEGIKRMYVNLENIFYYITVMNEPYTQPPMPEGSKDGILKG
                     MYKFRSSGLKDTDLHAQLFGSGTILNEVLKAQEALESEYGVAADVWSVTSYKELYRDG
                     CENERWNMLHPSELRRTPYIAQCVKDAPGVFVAASDYVMALPDSISQWLPRPLVSLGT
                     DGFGRSGSRKALRDFFEVDWRYIILAVLASLTWEKKIGTDIVEKAIKDLDINPGKPNP
                     LIA"
     gene            2845..4248
                     /locus_tag="VFJ67_01425"
     CDS             2845..4248
                     /locus_tag="VFJ67_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET7289069.1"
                     /translation="MAIEFKLPDLGEDIETGDVVRVYVSPGDRVEKEQALMELETDKA
                     ALEIPSPAAGVIKGVHVKEGDTIKIGQLLVTIEDGAEEGEKAAEVKTVKKEAPPKKVA
                     YEAMEPPKKEEPKETPEKKAGDKEAAPKDEPVKRPEKIQAREKTTEKATETGAGGEKP
                     AQIIPASPTVRRRAREMGIDITQVKGTGPGGRITDEDLKVPRTKAAPEETKPESVEKE
                     PEEKAPAAGAAASTDKYGETERAAMTRVRKLTAERLSESWKAPHVTQHDKADITELEK
                     LRKHYGKEVESLGGKLTMTSILLKAVSSALKLHPQFNASIDMESGEIIYKKYYNIGVA
                     VDTDRGLLVPVIKDADKKNIEELSVELRTLSEKARTKKITVEELQGGTFTITNLGGIG
                     GTYFTPVINSPEVAILGVSRSSVEAVYIDGEFQPRLMLPLSLSYDHRLIDGADAVRFL
                     RWIVEALEEPFKLSLEG"
     gene            4306..>4407
                     /locus_tag="VFJ67_01430"
     CDS             4306..>4407
                     /locus_tag="VFJ67_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010607389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7289070.1"
                     /translation="MTEEKKTQVAVIGAGPGGYPAAFRAADLGLDVTI"
BASE COUNT         1152 a         1123 c         1373 g          759 t
ORIGIN      
        1 cggagcgcgt cagcaagctc ctgaagatcc tacagataca cgcccagaaa aagggagtca
       61 ggctccagtt caccgcaaat acgccttaca taaacacatt cccggtcgag atgcagccgc
      121 cctatccggg aaaccaggcc atagaatgga atatcaggag ctttatcagg tggaatgcaa
      181 tggctatggt ggtgcgggcg aacaggcttg aggaggggat aggggggcat atctcaactt
      241 ttgcttctgc ggcggcactt tacgaagtag ctttcaacca ttttctaagg gccaggaacg
      301 ggaacaaaga gggggatata gtctattttc agggccacgc agcaccggga atttacgcga
      361 gggcgttcct cgaagggcgt ctcacgatag aaaacctgca aaacttcaga cgggaactcc
      421 agccgggcgg agggctgccc tcttatccgc acccgtggct tatgccgggg ttctggcagt
      481 tcccgaccgt atccatgggg ctgagtccga taatgtccat ataccaggcg cggttcagcc
      541 gctatctcga ggacagaggg ctgaaggagc attcggactc gaaagtgtgg gctttcctcg
      601 gggacggaga gtgcgacgag cccgaggcgc tgggcgcgat aaccctcgcc gcgagagaga
      661 agctcgacaa cctgatcttc gtcgtcaact gcaatctgca aagactcgac ggacccgtga
      721 gggggaacgg aaagataata caggagcttg aggcgatatt cagaggcgct gggtggaacg
      781 taataaaagt catctggggg agtaactggg acccgctcct cgccaaggac aaagacggcg
      841 tgctcgtaaa gcgcatgggc gaggtggtgg acggggagta tcagaaatac acggtcgaat
      901 cgggcgacta cattcgaaag cacttcttcg ggaccgaccc gaggctcctc gacatggtga
      961 aagacctcac ggacgaggag cttcagaagc tgagaagggg agggcacgac cccgaaaaaa
     1021 tctatcccgc gtacaaggcg gccgtcgaga acagaggctc gccgaccgtt atactcgcca
     1081 agaccgtgaa aggatacggg ctcggcgaag ccggggaagg caggaatatt actcaccagc
     1141 agaagaagct gaacgaggac gagctgaagg agttcaggac gaggttcgac ataccgatat
     1201 cggacgagga cgtcgtgaga gcgcctttct acagaccgca cgacgacagc ccggagataa
     1261 aatacataca ggagcggaga ctggagctgg ggggctacct gcccaagaga gtggttgact
     1321 gccagccgct ggagcagcca tcgccatcga tattcgagga gttttataaa ggctcgggag
     1381 agaggcccgt cgcgacaact atggccatag tacgcatgct ctcgaagctg ctcaaagata
     1441 agaacattgg gaagtacata gtcccgatag tccctgacga ggcccgcacg ttcgggatgg
     1501 aatcgctctt caggcaggtg ggcatatact cgcacgtggg ccagctatat gagcctgtcg
     1561 acgccgagac gctcttattc tacaaggagg cgcgggacgg gcagatactc gaagagggga
     1621 taacggaagc gggctcgatg tcgtcgttca tagccgcggg cacgtcctac gcgacctacg
     1681 gcatcaacat gatcccattc ttcatctttt actcgatgtt cggtttccag aggatagggg
     1741 acctcgtgtg ggccgcgggt gattcgaggg cgaagggctt catggtgggc ggcacatcgg
     1801 ggaggactac gctcgcgggc gaggggcttc agcaccagga cggacacagc cacctgctcg
     1861 catatcccgt gcctaactta aaagcgtacg accccgcttt tgcttacgag atcgcggtga
     1921 ttatcgagga aggcataaag aggatgtacg taaacctgga aaacatcttc tactacataa
     1981 ccgtaatgaa cgaaccttac actcagcccc cgatgcccga gggatcgaag gacgggatac
     2041 tcaaggggat gtataaattc aggtcttcag ggcttaagga cacggacctg cacgcccagc
     2101 ttttcgggag cgggacgata ctgaacgagg tcctcaaggc gcaggaggcg ctcgagagcg
     2161 aatacggagt ggccgccgac gtgtggagcg ttacgagcta caaggagctc tacagggacg
     2221 gctgtgagaa cgagaggtgg aacatgctcc atccgtcgga gttaaggcgg actccatata
     2281 tagcacagtg cgtgaaggac gccccggggg tcttcgtcgc ggcgtccgat tacgtgatgg
     2341 cccttcccga ctccatatcg cagtggctcc cgcggccgct cgtatcgctc ggcacggacg
     2401 ggttcggaag gagcggaagc aggaaggccc tgagggactt cttcgaggtg gactggagat
     2461 acataatcct ggccgtgctc gcgtcactga cctgggagaa gaagatcggg acggatatag
     2521 tggagaaagc tataaaggac ctcgacatca atccggggaa gccgaacccg ctgattgcct
     2581 agccttgccg tctttccaca gaatagagaa agaaaaaata atttcattcc ttcccacagc
     2641 tctacgggag tgatgcccgt tcaacccttc gacaagctcg gcacgaacgg gtttatcatc
     2701 gcggctggaa gccgctccta cggagagtag ttcgtttatg caggagcgcc atccctccga
     2761 ctccacatat ttatgctttc ctatttcgaa tctattccgg gatatgttat aaaattagtg
     2821 actatacttt gaggcagaac tcatatggca atcgaattta aactgcccga cctgggcgag
     2881 gacatagaga cgggggacgt cgtcagggtt tacgtgtcgc ccggggacag ggtcgagaag
     2941 gagcaggccc tgatggagct cgagacggac aaggccgcgc tcgagattcc ttcgcccgcg
     3001 gcaggcgtaa tcaaaggcgt gcacgtgaag gaaggggaca cgataaagat aggccagctc
     3061 ctcgtcacga tcgaagacgg cgcggaagag ggagaaaagg cggctgaagt gaaaaccgta
     3121 aaaaaagagg ccccgccgaa aaaagtagca tacgaggcca tggaaccccc gaagaaagaa
     3181 gagccgaagg agaccccaga gaagaaagca ggagataaag aagccgcgcc gaaagatgag
     3241 cctgtaaaga gaccggaaaa aatacaggct cgtgagaaga cgacggaaaa agcaacagaa
     3301 acgggggcag gcggggaaaa gcccgcgcag ataatcccgg cgtctcccac agtgaggagg
     3361 cgggcgcggg aaatgggaat agatataact caggtgaagg gcaccgggcc gggcggacgg
     3421 atcacggatg aagacttaaa ggtcccacgg acgaaggcgg ctcccgaaga aacgaagccc
     3481 gaatccgtag aaaaggagcc cgaggaaaag gcccccgcag ccggagccgc agcatcaacc
     3541 gataaatacg gcgagaccga gcgcgcggcg atgacgaggg tgaggaagct cacggcagag
     3601 cgcctgagcg aatcgtggaa ggcccctcac gttacgcagc acgacaaggc ggacattacg
     3661 gaactcgaaa agctgaggaa gcactacggg aaggaagtgg agagcctggg cgggaagctg
     3721 acgatgactt cgatactcct caaagccgtc tcctccgcac tgaagctcca cccgcagttc
     3781 aacgcaagca tagacatgga aagcggggag atcatctaca agaaatacta caacatcggc
     3841 gtcgcggtcg atacggacag ggggctgctc gtcccggtga taaaggacgc cgacaaaaag
     3901 aatatagaag agctctctgt agagctgagg acgctctccg agaaagcgag gacgaagaag
     3961 ataaccgtcg aggagcttca gggagggacg ttcacgataa cgaacttagg agggatcggc
     4021 gggacttact tcacgcccgt aatcaattcg cccgaggtcg cgattctcgg cgtttcgcgc
     4081 tcgtctgtcg aggcagtgta tattgacggt gagttccagc cgaggctcat gctgccgctc
     4141 tcgctttcat acgaccaccg tctcatcgac ggcgcggatg cggtgaggtt cctgagatgg
     4201 atcgtagagg cgctcgaaga gcctttcaag ctctctctcg aaggctgaca cacacgtagt
     4261 gtgttttatg tttgcgtcac aggcattttt ttaataaata cctatatgac cgaagaaaag
     4321 aaaacacagg ttgcagtgat cggggccggc ccgggggggt atccggccgc gttccgcgcg
     4381 gccgacctcg gcctcgacgt caccatc
//
LOCUS       DASSJH010000066         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_232985, whole genome shotgun sequence.
ACCESSION   DASSJH010000066 DASSJH010000000
VERSION     DASSJH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232985"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFJ67_01435"
     CDS             <1..390
                     /locus_tag="VFJ67_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7289071.1"
                     /translation="LDDESQIYFDDYNDLRMDKWYKGRVVLTGDSAHAVLPTAGGGVS
                     MAIESAAVLADELSRADSKYIAHALESYESRRRPRVVKIQDQSRMMGKVAFADSRVLS
                     VLRNLVMRFYSNRLHIKYWDSMLKEPI"
     gene            609..2039
                     /locus_tag="VFJ67_01440"
     CDS             609..2039
                     /locus_tag="VFJ67_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HET7289072.1"
                     /translation="MARLARISILSIIFSVFLTAFFQSAVAQQYGKNSLAPLVKQLSP
                     SVVNISTTSVSKGRSFSYESPFGGKEGDQFNDFFDKFFGESPEREFKGMGLGSGFIFS
                     EDGYIITNNHVVERATDIKVILEDNEIYHAEVVGTDPKTDLALLKIEPKRKLPAVKFG
                     NSDNLEIGDWVLAIGNPFGLGHTVTAGIISAKGRSLGLGSYDDFIQTDAAINPGNSGG
                     PLFNFSGEIIGVNTAIIAGGQGIGFAIPINMAKSVVAQLRDSGKVVRGWIGVYVQQIT
                     PEIAESINLGDAHGALVSDVTPGGPAELAGVQRGDIIVEFNGKSIDDMPELPRYVASY
                     APGTQTQLKVLRNGEPKVLNIKLGELPEEVAQSSRKLTGKAVEEGLGLIVQEITPQVQ
                     NTFETNVSEGVIITNVAPGSTAANAGLEPGDVILEINKNKVANLDNYKKSMDSISEGQ
                     NVLFLVKRGTNTIYVALKFEGENGKG"
     gene            2026..2490
                     /locus_tag="VFJ67_01445"
     CDS             2026..2490
                     /locus_tag="VFJ67_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7289073.1"
                     /translation="MGKVEERLNELGIKIPETPVPLGSYKPASIAGNLIFISGQLPLS
                     NGKLLYQGKVGSDVTAEDAAQAARASAINALAALSRELDGLDRVRKIVKVTGYVASAA
                     GFYNQAAVVNGASDLFFQVFGEDGRHARAAVGVAELPMNAPVEIELVAEITP"
     gene            2534..>2758
                     /locus_tag="VFJ67_01450"
     CDS             2534..>2758
                     /locus_tag="VFJ67_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289074.1"
                     /translation="MGRPPTVFIYTDYPSEALDTDAVAERLNGLGIPTVTRGDLFQFL
                     GNSETARRIAGLMVRSRVPDIGEPLDALRRE"
BASE COUNT          678 a          714 c          794 g          572 t
ORIGIN      
        1 ctcgacgatg agtcccagat atatttcgat gattacaacg acctcagaat ggataagtgg
       61 tataagggca gggtcgttct cacgggggac tccgcacacg ccgtgctgcc cactgcggga
      121 ggcggcgtat ccatggcgat agaatcggcc gcggtactgg cggacgagct ttcgagagcg
      181 gattccaaat acatcgcgca cgcgcttgag agctacgagt cgaggagacg cccgcgcgta
      241 gtcaagatac aggaccagtc gaggatgatg ggcaaggttg cgttcgccga cagccgggtc
      301 ctctccgtgc tcaggaactt ggtcatgcgt ttttactcga acaggctcca tattaaatac
      361 tgggactcca tgttgaaaga gccgatataa aattgccgta actatccccg tccgcttgta
      421 tttccgacat tcatcctcgt aattttttct gcatgggacc gaatcttagg tatacttttt
      481 tcgttgttca agtacaagcc ggcaaataat aatttataat ttacataccc ggggatatat
      541 gattgagagc ttggctaatg gctaacccgg acaaaaatca atcattaaat cgggaggtag
      601 taaggtcaat ggcaaggttg gctaggatat cgatactttc cataattttc tcggtcttcc
      661 tcactgcgtt ctttcaatcg gcggtggcac agcagtacgg aaaaaactcc ctcgcgccgc
      721 tcgtgaagca attgagcccg tcggtcgtta acataagcac caccagcgtc tccaagggaa
      781 ggagcttttc atatgagtcg cctttcggcg gaaaagaagg cgatcagttc aacgatttct
      841 tcgacaagtt cttcggcgag tctcccgaaa gggaattcaa gggaatgggg ctcggctccg
      901 ggttcatatt cagtgaagac ggctacatca ttacaaacaa ccatgtcgtg gaaagggcaa
      961 ctgatataaa ggtcatactt gaggacaatg agatttacca tgctgaagtt gtcgggaccg
     1021 accccaagac cgatctcgct ctccttaaaa tagagcccaa gaggaaatta ccggccgtca
     1081 aattcgggaa ttccgacaac ctcgagattg gagactgggt gctcgccata ggcaatccgt
     1141 tcggactcgg gcacacggtt acggccggga taatcagcgc gaaaggcagg tcgttagggc
     1201 tcggctccta tgacgacttc atccagacgg acgcggctat caacccggga aacagcggcg
     1261 gcccgctctt taatttcagc ggcgaaataa taggcgttaa tacggccata atagcgggag
     1321 gtcagggcat cggcttcgca atacctatta acatggcgaa gagcgtggtc gcgcagctca
     1381 gggacagcgg caaggtggtt cgcgggtgga taggcgtgta cgtccagcag atcactccgg
     1441 aaattgctga gagcatcaac ctcggggacg cgcacggtgc gctcgtttcg gacgtaactc
     1501 ccggcggccc ggcggagtta gccggtgttc agaggggcga tataatcgtc gagttcaacg
     1561 gcaagtccat agacgatatg ccggagctcc cgagatacgt cgcgtcttac gccccgggga
     1621 cgcagaccca gctgaaggtc ctcaggaacg gagagccgaa ggttctaaac ataaaattgg
     1681 gggagcttcc cgaagaagta gcgcagagct cgagaaagct cacgggtaag gccgttgagg
     1741 aaggtctcgg actgatagtg caggaaataa cccctcaggt tcagaatacg tttgagacga
     1801 atgtttccga gggcgtgata attacgaacg tcgctccggg tagcaccgca gccaacgccg
     1861 gcctcgagcc cggagacgtc atactcgaaa taaacaagaa taaggtcgcg aacctcgata
     1921 actataagaa atccatggat tcgatatccg aggggcagaa cgttctcttc ctcgtgaagc
     1981 gcgggactaa caccatctac gtcgctctca agttcgaggg ggaaaatggg aaaggttgaa
     2041 gagaggctga acgagctcgg aataaaaatc cccgaaacac ctgttccgct cggctcgtac
     2101 aaaccggctt ccatcgccgg gaatctgata tttatttcgg ggcagctccc tctttcgaat
     2161 ggcaagctcc tgtatcaggg aaaggtcggc tcggacgtaa cggctgagga cgccgcacag
     2221 gccgcgaggg cctcagcgat aaatgcgcta gccgctctga gcagggaact cgacggcctc
     2281 gacagggtca ggaagatcgt gaaggtgacg ggttacgtag cgagcgcggc gggcttttat
     2341 aatcaggccg ccgtcgtgaa cggagcttcc gacctctttt ttcaagtctt cggggaagac
     2401 gggcgtcacg cccgcgccgc cgtaggagtg gccgagctcc cgatgaacgc ccccgtggaa
     2461 atagagctcg ttgccgagat aaccccctga ggggcttgcg ggcaaagacg atacccggtt
     2521 aagttctttc cctatgggcc gacccccgac cgtatttata tacaccgatt atccttccga
     2581 agcgctcgat acggacgcag tagccgaacg cctgaacggg ctcggtattc cgaccgtgac
     2641 caggggagac ctgtttcaat tcctgggaaa ttcagagaca gcccgccgca ttgccgggct
     2701 catggttcgg tcaagggtcc cggacatcgg ggagcctctc gatgccctgc gccgggag
//
LOCUS       DASSJH010000067         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_124450, whole genome shotgun sequence.
ACCESSION   DASSJH010000067 DASSJH010000000
VERSION     DASSJH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124450"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFJ67_01455"
     CDS             <1..211
                     /locus_tag="VFJ67_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289075.1"
                     /translation="FDSWLNKAIDMQRVINNNEILNVILIIHPPSKNAEFRRLYFITG
                     GCVNERCAGGGEFDGEVRCLIFRSS"
     gene            619..2163
                     /locus_tag="VFJ67_01460"
     CDS             619..2163
                     /locus_tag="VFJ67_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET7289076.1"
                     /translation="MVKAKIVCAALLLLSGLVPAWAGDNPAPMFASEEPGAPHDPGDV
                     VMPVEEPATVSSTEDTGTGLAKTVMPNLRPPGFKTYMEDTAIWYGAQWAARLWWVRDK
                     NVKIFNGGFHTFWSNLTSDPLWNDQDDFVVNWVLHPFFGMMSYQFYRARGHSVWASAL
                     GSVIQSTLFEYAIEGWAVRPSGIDLIVTPALGVPLGWTMEQLSDWLIRQDSKAAHIAA
                     YITNPTRTFVKDTKFGLINPVTGAFQFQGQFTISTTKGKALELAYPKFFEPPLPLGRV
                     GLDVELITLEKKLGGEFILYPLRLEFPSESNYWGVYMVLPYGGVNNVMDGDTEIRDGY
                     EFGNLKVGVKSLAAKSKNFAIAGGLDVYFPTSFTDDQQRLQQIIKYRRDFQMYLYKAT
                     TISPYISAGAWNGVFSLQGAVGTDFMFHAEEFAEQDFEFRVNYHAAAGLNVPMTGSPI
                     VFCEFDGYTIPTYDGAGGDTDLFISPGIRFGRKISPGFTVQIPVYGPSNDIANADYVF
                     DFQLRF"
     gene            complement(2385..2780)
                     /locus_tag="VFJ67_01465"
     CDS             complement(2385..2780)
                     /locus_tag="VFJ67_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7289077.1"
                     /translation="MKNLILYLAALSIIFQAYTARAQQSAPAPPSDVQKVEVVVDSYS
                     FTPDIIKVKAGKPVELTLRSVTSLIPHNFTIDDPASGLNVDADIPSGKDVTVTFTPLK
                     TGKFQFYCGKKNILGSHLKKGMKGTLEVE"
     gene            complement(2836..3117)
                     /locus_tag="VFJ67_01470"
     CDS             complement(2836..3117)
                     /locus_tag="VFJ67_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289078.1"
                     /translation="MIRRLLLISLTVVSILPAVCCSTLKTGKDIAIDNPMSESKTKQS
                     LNDARQQVEKSRYALDRCLDANSGDQTRCQSEKAAYDKDVENYVSLQSQ"
BASE COUNT          767 a          933 c          991 g          778 t
ORIGIN      
        1 gtttgattca tggctgaaca aggcaattga catgcagagg gtaataaaca ataacgagat
       61 attgaatgta atcctcatca tacatcctcc ttcaaaaaat gctgagttcc ggagattata
      121 tttcataacg gggggttgtg tcaatgaaag atgtgcgggc gggggagaat ttgacggaga
      181 ggtgaggtgt ctcatcttcc gttcgtcttg agcgctcata tagtgtacag tcgaatgatg
      241 taatatttat tgtcattgcg agaacgattt cgactgaggg aggaatcgga cgccttcgac
      301 taatcgcgaa ggagcacatt catcaatgca cataagttac cgaaaccaaa ttgagaaacg
      361 aagcgctgca atctcaagac aacaaagaga tcgtagaatt ccttctcgca aaaacagagt
      421 agcatggaat atagtctgag cacacaatgt acgctcatgt acgattcctc caactctact
      481 gatcgaaccg gcggatgcgt gtgcatgttt tcgccgtccc gtaaatattt ccttttattt
      541 cggctcaatt attgttaaaa tgtgctatgc gcccggacgc gattcccgcc cgacacgtcc
      601 ggacgcttag aggaggatgt ggtgaaggcg aaaatcgtat gcgctgcttt gctcttgctg
      661 tcagggctcg tccccgcctg ggcaggggat aatcccgctc ccatgttcgc gtctgaagaa
      721 ccgggcgctc cgcacgatcc gggagatgtc gtcatgcctg tggaggagcc cgccacggtg
      781 agctccaccg aagacacagg cacgggcctg gcgaagaccg tcatgcccaa cctcaggccc
      841 cccggcttca agacctacat ggaagacacc gcgatctggt acggcgcgca gtgggccgcg
      901 aggctctggt gggtgaggga caagaacgtc aagatattca acggagggtt ccatacattc
      961 tggagcaacc tcacgagcga tccgctctgg aacgaccagg acgatttcgt cgtcaactgg
     1021 gtcctccacc ccttcttcgg catgatgtca taccagttct acagggcgag gggccacagc
     1081 gtctgggcgt cagccctggg ctcggtcata cagagcacgc tttttgaata cgcgatcgag
     1141 ggatgggcgg tcaggccctc gggcatcgac ctcatagtca ccccggcgtt aggcgtcccg
     1201 ctcggatgga ccatggagca gctctccgac tggctcatca ggcaggactc caaagccgcc
     1261 cacatagcgg catacataac aaatcccact agaactttcg tcaaggacac gaagttcggg
     1321 cttatcaatc ccgtaacggg cgcgttccag ttccagggcc agttcacgat atcgaccacg
     1381 aaagggaagg cgctcgagct cgcctacccg aagttcttcg agccgccgct gccgctcggc
     1441 agggtggggc tggacgtgga gctgatcacg ctcgagaaga agctcggtgg cgagttcatt
     1501 ctctatccgc tcaggctcga gttcccttcc gagagcaatt actggggcgt gtacatggta
     1561 ctgccttacg gcggcgtgaa caacgttatg gacggggata cggagatcag ggacgggtac
     1621 gagttcggga acctcaaggt cggcgtaaag tcgctcgcgg cgaagtcgaa gaacttcgcc
     1681 atagccggcg ggctcgacgt gtacttcccg acatcgttca cggacgacca gcagaggctc
     1741 cagcaaataa taaaatacag aagggacttc cagatgtacc tttacaaggc gaccacgatt
     1801 tcgccttaca tctcggcggg cgcctggaac ggagtgttca gcctccaggg cgctgtcggc
     1861 acggacttca tgttccacgc cgaggaattc gcggagcagg acttcgagtt cagggtcaac
     1921 tatcacgcgg ccgcgggcct gaacgtcccc atgacggggt cgccgatagt attctgcgag
     1981 ttcgacgggt acacgatccc gacatacgac ggcgcgggcg gggacaccga cctcttcatc
     2041 tcgccgggca taaggttcgg gcgtaaaatc agtcccgggt tcacggtcca gatcccggta
     2101 tacggccctt ccaacgacat agccaacgcc gattacgtct tcgacttcca gttgaggttt
     2161 tagtgctcct ttctttattt aatttgtcat tgcgagggag cgttttatcc cggcgtagcc
     2221 ttttggcgaa gccgaatgcg accgcggcaa tctcatgatt aaagcgagat cgtcacggct
     2281 ccttcgcgca aggcttcgga gcctcgcgaa gatagaaaaa tggtgtcatt cccgaggcga
     2341 tgcaagatac atgttgcttc tgcggaatcg acccgaaacc acgctcactc cacttcgagc
     2401 gtgcctttca ttcccttctt taggtgggag cccagtatgt ttttcttccc gcagtagaac
     2461 tggaacttcc ccgtcttgag cggtgtgaac gtcacggtga cgtcctttcc cgacggtata
     2521 tccgcgtcca cgttcaaccc cgacgccgga tcgtcgatag tgaaattatg cgggatgagg
     2581 gacgtaacgc tcctgagcgt gagctcgacc ggcttcccgg ccttaacttt tatgatatcg
     2641 ggcgtgaaag aataactgtc gacgactacc tccaccttct gaacgtctga cggaggcgcg
     2701 ggcgcgctct gctgcgcgcg ggctgtatag gcctgaaaga taatcgataa agcggcaagg
     2761 tatagaatca ggtttttcat ttttaatccc cccgtccgtg atgtatttat ataaccacag
     2821 gtgtcgtgcg ccggctcact gcgactggag cgaaacgtag ttctcaacgt ccttgtcgta
     2881 ggcggctttc tcgctctggc acctcgtctg gtcgccggag ttggcgtcga ggcaccggtc
     2941 gagcgcgtac cgcgatttct cgacctgctg cctggcgtcg ttaagcgact gcttcgtctt
     3001 cgattcgctc atcgggttgt cgatggcgat gtccttacca gtcttgagcg tgctgcagca
     3061 gacggcaggg aggatggaga cgacggtcag cgaaatgagg agaaggcggc ggatcacttt
     3121 attacccccg tggatatttg ttggaccggc accccggtca cggacaacat tataatcaat
     3181 atttggctgc ggggctatcg cggttgagag aaaattgagg ccggttacag gcgtcccggg
     3241 cgggcggcgg taaaacactc cgggcgggtg aggtcgtcac cctcccggaa ttccatcatc
     3301 gtccttgtag gagttcggat gtagtgtaaa actagagcgt ggaggatcag gcgccgaaat
     3361 ggctgaagta atcgccgata agcgagaaag ccttttcgac gttttcctgg gccattgtgc
     3421 gtgcgtcctt cgtgaacttc tcggacatgg tcttaaccgt ctgcatctg
//
LOCUS       DASSJH010000068         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1530347, whole genome shotgun sequence.
ACCESSION   DASSJH010000068 DASSJH010000000
VERSION     DASSJH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530347"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..656)
                     /gene="greA"
                     /locus_tag="VFJ67_01475"
     CDS             complement(186..656)
                     /gene="greA"
                     /locus_tag="VFJ67_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET7289079.1"
                     /translation="MERVPMTPQGYKKLQEELRRLKTVERYEVIKLIEFARSLGDLSE
                     NAEYETAKQRQSFVEGRIQELESKIGRAEIIDPAELKDKNRVTFGVKVKLENVDTGET
                     VTYQLVGPDESEPDHGLISITSPIGKALIGKQVDDDVQVQAPGGMREFVVLEIS"
     gene            complement(769..>2376)
                     /gene="carB"
                     /locus_tag="VFJ67_01480"
     CDS             complement(769..>2376)
                     /gene="carB"
                     /locus_tag="VFJ67_01480"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7289080.1"
                     /translation="FEAYTPYLYSTYEREDEAPPTDRSKVVILGGGPNRIGQGIEFDY
                     CCVHASFSLREEGYEAIMVNCNPETVSTDYDTSDRLYFEPLTLEDTLSIIGRENPEGV
                     IVHLGGQTPLKLAIPLEERGVRIIGTSPDSIDLAEDRERFRDLVVELGLRQPESGIAR
                     SQEEAIGIANDIGYPIVVRPSYVLGGRAMEIVYTEDSLRRYIREAVRVSPNLPVLIDK
                     FLKDAKEVDVDALSDGRTVVIGGVLEHIEEAGVHSGDSAMVLPPFSIDPQVIREIKRQ
                     TKELALALKVKGLVNIQFAVKDNLVYILEVNPRASRTVPFVSKAIGVPLAKLGTKVMI
                     GKTLEELGFTREIVPIHYCVKESVFPFIKFHGVDTILGPEMKSTGEVMGIDMDLSMAF
                     AKAQIAAGSDLPLSGTAFISVKDEDKPKLSLFIKELEGLGFGIMATAGTAAYLESIGV
                     RCLVVKKVIEGRPHVVDHIKNGEIQLVVNTTFGEKEVEQSYSIRRTALVHRVPYFTTI
                     SAARAAVGAIEVLIRKGLDVKAIQDYY"
BASE COUNT          470 a          700 c          605 g          601 t
ORIGIN      
        1 aagagtctat acggagatta tccgaggaac tcggatgagc tcagacattt tatagagctc
       61 gaaacgacgg ctgattttcc tgtccacccg cttggcgggg aatacattta cgacccgaat
      121 accgggaaag tggaatcatc cgtcgacata gagtgagttg tcattgaaag ccgcgggatc
      181 ccgcgtcagc ttatctccag aacgacgaac tccctcatgc cgcccggagc ctgaacctga
      241 acgtcgtcat ccacctgttt tccgatcagc gccttgccta tgggagaagt gatggagatg
      301 agaccgtgat cgggctcgga ttcgtccgga ccgacgagct gataggttac ggtttccccc
      361 gtatcgacgt tctcgagctt tacttttaca ccgaacgtta ccctgttctt gtcctttagc
      421 tcggccgggt ctattatctc ggccctgcct atcttgctct cgagctcctg tatcctcccc
      481 tctacgaagg actgcctctg tttcgccgtt tcatactccg cgttctccga taggtctccg
      541 agggacctcg cgaactctat cagctttata acttcatatc tttccacggt cttcagcctt
      601 cggagctctt cctgaagctt cttatatccc tgcggagtca tcggcactct ctcaatggtc
      661 atattaagta ttcctcctgg agctgctaac ggagcgggaa aggaaatcgc cttgcgcgga
      721 ttaaattata tattgtcagg ataaacatga tattcaatta tacaacacct aataatagtc
      781 ctgaatagcc ttgacgtcga gacctttcct gatgagcacc tcgatcgctc cgacggctgc
      841 cctggccgct gagatcgtcg taaagtatgg aaccctgtga acaagcgccg tgcgccttat
      901 ggagtaggac tgctccactt ctttctctcc gaaagtcgtg ttcactacca gctgaatctc
      961 cccgtttttg atgtgatcga ccacgtgcgg gcggccttcg atgaccttct ttaccacgag
     1021 acaccgcacg cctatgcttt cgagatatgc cgcggtccct gcagtcgcca tgatgccgaa
     1081 tcccagacct tcgagttctt ttataaacag cgagagcttg ggcttgtcct cgtctttcac
     1141 gcttatgaac gccgtccccg atagggggag gtcgctgccg gcggcgatct gggcttttgc
     1201 gaaagccatg ctgaggtcca tgtcgatgcc catgacttca cccgtggatt tcatctcggg
     1261 tccgaggata gtgtccacgc cgtgaaattt gatgaacggg aaaacggact ccttgacgca
     1321 gtagtgtatg gggacgattt cccttgtaaa tccaagctcc tcgagtgtct tccctatcat
     1381 gaccttggtc ccgagcttgg cgagagggac tccgatggct ttactgacga agggcactgt
     1441 cctgctcgcg cgggggttta cctcgagtat gtagaccagg ttgtccttga ccgcgaattg
     1501 tatattgacg agccccttta ccttgagcgc gagcgcaagc tctttcgtct gacgcttgat
     1561 ttcacgtatc acttgcggat cgatcgagaa gggaggaagg accatggcgc tgtcgcccga
     1621 gtgcaccccc gcctcttcta tgtgctcaag cacgcctccg atgacgaccg tcctgccgtc
     1681 ggacaaggcg tcgacgtcca cttccttggc gtctttcagg aacttgtcta taagcacggg
     1741 cagattgggc gagaccctta ccgcctccct gatataacgc ctgagcgaat cctccgtata
     1801 tacgatctcc atggcgcggc ccccgaggac ataagaaggc ctcacgacga tcggatagcc
     1861 tatatcgttc gctatcccga tcgcttcttc ctggctcctc gctatcccgc tctcgggctg
     1921 tctcagcccg agctcgacca ctagatccct gaacctctcc ctgtcctcgg cgaggtctat
     1981 gctgtcgggc gaagtgccta tgatcctgac gcccctctcc tcaagcggta tggcgagctt
     2041 gagcggggtc tggcccccga ggtgtacgat gacgccctca gggttctccc tcccgattat
     2101 tgagagggta tcctcgagcg tgagcggctc gaagtagagc ctgtccgaag tatcgtaatc
     2161 cgtgctgacc gtctccgggt tgcagttcac cattatagct tcatatccct cttccctgag
     2221 ggagaaagaa gcgtggacgc agcagtaatc gaactctatg ccctgcccga tcctgttcgg
     2281 tcccccgccc aggatcacta ctttggacct gtcggtcggc ggggcctcgt cctctctctc
     2341 gtatgtcgag taaaggtaag gcgtataagc ctcgaa
//
LOCUS       DASSJH010000069         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_989562, whole genome shotgun sequence.
ACCESSION   DASSJH010000069 DASSJH010000000
VERSION     DASSJH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989562"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1383)
                     /locus_tag="VFJ67_01485"
     CDS             complement(127..1383)
                     /locus_tag="VFJ67_01485"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET7289081.1"
                     /translation="MQELFNSEAFREATLQFERASKLIPDDDGLEERFRLPQRTLVVS
                     CPVKMDDGTTRVFSGYRVQHNQTLGPYKGGIRYHPDVDMGEVAALAMNMTWKCGLAEL
                     PFGGAKGGVSVDPRELSQGELERLTRRYTAEILPIIGPTRDIPAPDLGTSPQVMAWIM
                     DTYSMAQGYTVPSIVTGKPVIIGGSLGREEATGYGVAYVTDDALRKFKRYTENIKVVI
                     QGFGNVGSFTARKLNEMGITVVAVSDVYGGIYNRNGLPADELFRTVREKGTVTGYADG
                     DKVTNDELLTLECDVLIPAALGRAITKENAPRLRCGMVVEGANNPTTAEADDILRERG
                     IPVIPDILANAGGLIVSYFEWVQGLQEYYWSMEAVHDDLKKRMKNTFIKVAEYAEQEK
                     IPMRLAALTLGIRHVSRAMKLRGLYP"
     gene            complement(1597..2670)
                     /locus_tag="VFJ67_01490"
     CDS             complement(1597..2670)
                     /locus_tag="VFJ67_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HET7289082.1"
                     /translation="MTRKILGITIILLAFLTAGVSRAEDETMKKAAALFKPIPAAAPE
                     NKENPVTPDKVELGKMLYFDPRLSASDLISCNTCHNMGTGGVDLQETSVGHKWQKGPR
                     NAPTVLNSVFNIAQFWDGRAKDLEEQAKGPMQATVEMSNTPERVTQTLKSMPGYVEHF
                     SKTFPGETDPVTFDNAVKAIEAFESTLLTPDSPFDKYIKGDSTALTDNQKAGLALFMD
                     KGCAGCHNGINVGGGGYFPFGLVEKPADEVRPPGDMGRFAVTKTKSDEYVFKVPTLRN
                     ITLTAPYFHSGKVWDLSDAVAIMGEAQLGTKLTDEEVARIVDFLGALTGTQPEVVYPV
                     LPPNGPDTPRPVLDIPDITGKTK"
     gene            complement(2711..3016)
                     /locus_tag="VFJ67_01495"
     CDS             complement(2711..3016)
                     /locus_tag="VFJ67_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289083.1"
                     /translation="MSEDFGVGYTTYLKYATAAALMVAWVLAAWTGFMLGVVQYSTGG
                     ENELLLGYTRAEWGILHSWLSVIAVFITAVEIVLTRKFLTEGLRYLAASESGQADSR"
     gene            complement(3050..>3383)
                     /locus_tag="VFJ67_01500"
     CDS             complement(3050..>3383)
                     /locus_tag="VFJ67_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289084.1"
                     /translation="AYLTGEPAEEVVENTPGISENLIEEHEEAAEKAFILVEAVGGLA
                     LIGLIARRFSRNLGNTLAVATLAGLIAGGGLVAYTANLGGKIHHQEIRGDAQAPTSPA
                     GSTNEDRD"
BASE COUNT          574 a         1055 c          945 g          809 t
ORIGIN      
        1 gaggacccgg gctgcgggca gcgatctcag ggacgggagg tgaaatgttt ccgtcaccct
       61 gccgcagccg attattccta ctttaacagt tcgcgacata tttgtcttta tcaccgggcc
      121 agggcgctac ggataaagcc cgcggagctt catcgcccgg gacacgtgcc ttatgcccag
      181 tgtaagggcc gcgagccgca tcggaatttt ttcctgctcg gcgtattccg caacctttat
      241 gaacgtgttc ttcatgcgct tcttgagatc gtcgtgcacc gcctccatcg accagtaata
      301 ttcctggagc ccctgcaccc attcgaagta agagactatg agcccgcccg cgttagccag
      361 tatgtcggga attacgggta tgccccgctc cctcagtatg tcgtccgcct ccgcagtggt
      421 cgggttgttc gccccctcca cgaccatgcc gcacctgagc ctcggagcgt tctccttcgt
      481 tatcgccctc ccgagcgcgg ccggtatcag cacgtcgcat tcgagcgtga ggagctcatc
      541 gttagtcacc ttgtccccgt ccgcgtaccc ggtcactgtc cctttctccc tgacggtcct
      601 gaaaagctcg tccgcgggga ggccgttccg gttgtagatg cccccgtaaa cgtcgctcac
      661 ggcgacgacc gtgataccca tttcgttgag cttgcgcgcg gtgaagctgc ccacgtttcc
      721 gaacccctgt atcacaactt ttatgttctc tgtgtacctc ttgaatttcc tgagcgcgtc
      781 gtccgtcacg taggcgacgc cgtatcccgt ggcctcttcc ctcccgaggg agcctccgat
      841 tatgaccggc ttgcccgtga ctatggacgg gaccgtatag ccctgagcca tgctgtaggt
      901 gtccatgatc caggccatga cctgtgggct ggtcccgagg tcgggcgcgg gtatgtccct
      961 ggtaggcccg atgatgggga gtatctcggc cgtgtacctc ctcgtgagcc tttcgagctc
     1021 gccctgcgag agttcccgcg ggtctacgga cactccccct ttggcgcccc cgaacggcag
     1081 ctcggctagg ccgcacttcc acgtcatgtt catagcgagc gccgccacct cgcccatgtc
     1141 cacgtccggg tggtagcgta tcccgccctt gtaggggccg agcgtctggt tgtgctgcac
     1201 tctgtatccg ctgaataccc tcgtcgtgcc gtcgtccatc ttcacggggc agcttacgac
     1261 gagcgtcctc tgcggcagcc tgaacctctc ttcgaggccg tcgtcgtccg ggatgagctt
     1321 gctcgccctc tcgaactgga gggtcgcttc cctgaacgct tcggaattaa aaagctcttg
     1381 catgtaaaac ctcctgcggg gatgatggat gaggcgcgga tccgggattc gtaatccgca
     1441 tggaagacct gtttaatccc gcttgagaga attatataga aactccggca ggatagcgat
     1501 aggaaaatta cacactgcag aacggatgac catcctcggg ggggcagccg acgggagatt
     1561 ggaccgcccc ctgcggaacg ggatgaaaaa tgcggactat ttagtctttc ccgtaatgtc
     1621 cggtatgtcg agcacggggc gcggagtgtc ggggccgtta ggcggcagca ccggatatac
     1681 gacctcgggc tgcgttcccg tgagcgctcc gaggaaatcg acgatcctcg ccacttcctc
     1741 gtccgtgagc ttcgtcccga gctgcgcttc tcccattatg gccacggcat cgctgagatc
     1801 ccacactttt cccgaatgga aatagggcgc ggtgagcgta atgttgcgga gcgtcgggac
     1861 cttgaacacg tactcgtcgc tcttggtctt ggtgacagcg aaacgtccca tatcgccggg
     1921 aggccttacc tcgtcggccg gtttctctac gagcccgaac gggaagtaac ctcctccgcc
     1981 gacgtttatg ccgttgtgac acccggcgca gcccttgtcc atgaacaggg cgagccctgc
     2041 tttctgatta tcggtcagcg ccgtgctgtc gccctttatg tatttgtcga acggggagtc
     2101 gggcgtaagg agcgtggatt cgaacgcctc gatcgctttc acggcgttgt cgaacgttac
     2161 gggatcggtc tcgccgggga acgtcttact gaaatgctcg acgtatccgg gcatgctctt
     2221 gagcgtctgt gtcaccctct cgggcgtgtt gctcatctct acggtcgcct gcatcggccc
     2281 cttggcctgc tcctcgaggt cctttgcgcg gccgtcccag aactgcgcta tgttgaatac
     2341 ggagttgagc acagtgggag cgttccgggg acctttctgc cacttgtgcc cgacggaggt
     2401 ctcctggagg tcgacgcctc ccgtgcccat gttatggcag gtgttgcagc ttatgaggtc
     2461 cgaggcagag agtctcggat cgaaatagag catcttcccg agctcgacct tgtcgggcgt
     2521 aaccgggttc tccttgttct caggagcagc ggccggtatc ggcttgaata gagcggccgc
     2581 cttcttcatc gtctcgtctt ccgctcgtga tacccccgca gttaaaaaag cgagaagtat
     2641 aatcgttatt ccgagtattt ttcttgtcat tttcctgcct ctctttttat tgttgtaaat
     2701 attctttctg tcatcgcgag tccgcctgtc cgctctctga cgcggcaaga tacctcaggc
     2761 cttcggtgag gaacttccgt gtgaggacta tctccacggc cgttatgaaa acggctatca
     2821 cgctcagcca tgaatgtaga ataccccatt cggccctcgt atatccgagg aggagctcat
     2881 tctcgcctcc tgtggaatac tgtactacgc ccagcataaa acccgtccac gctgccagca
     2941 cccacgcgac catgagcgct gcggcggttg cgtacttaag atacgtcgta tagccgactc
     3001 cgaaatcctc actcattctt tttctccctg tatttatctt tctctggatt cagtccctgt
     3061 cttcgttcgt actgccggcg ggtgacgtcg gggcctgagc atcgcccctt atctcctggt
     3121 ggtgtatctt ccctccgagg ttggccgtgt acgcgacgag ccctccgccc gctatgagcc
     3181 ccgcgagggt tgcgaccgcg agcgtattgc cgagattcct gctgaaccgc cgggcgatga
     3241 gccctatcag ggcgagacct ccgacagcct cgacgagtat gaaggccttc tcggccgcct
     3301 cctcgtgctc ctctatcaga ttctcggaaa tccccggcgt gttctcgacc acttcctcgg
     3361 caggctcccc cgtgagatag gcc
//
LOCUS       DASSJH010000070         5862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_342951, whole genome shotgun sequence.
ACCESSION   DASSJH010000070 DASSJH010000000
VERSION     DASSJH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342951"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..490
                     /locus_tag="VFJ67_01505"
     CDS             170..490
                     /locus_tag="VFJ67_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7289085.1"
                     /translation="MQGKKLYIGNLAYNTTAEELTELLSKYGTVSDLRLIGDKGFGFA
                     EMGTQQEAEKAISELDGYEFKGRPLKVNEARKPTDKPRGGRSGGGGGYGGGGGGGGGY
                     GRRR"
     gene            797..1516
                     /locus_tag="VFJ67_01510"
     CDS             797..1516
                     /locus_tag="VFJ67_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7289086.1"
                     /translation="MPKIDEIAPGVYRISVTYEEIGLQFNHFLVKDDEPLLFHSGLNG
                     MFNEVYEGVSKLIKPSDLRWVGFSHFESDECGSLNRWLEAAPRAEAVCSSLGALVSVN
                     DFAKRPARGMNDGETLRTGDKVFRFCSTAHLPHGWDAGVFYEETDKTLFCSDLFTHFG
                     EVEPITESDVVGRAADSLRNMQQSPFAYYIPYTPQTKGILSRLASLDTKTLAIMHGSS
                     YTGDCGKALLDLSSVIEEELG"
     gene            1583..2617
                     /locus_tag="VFJ67_01515"
     CDS             1583..2617
                     /locus_tag="VFJ67_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET7289087.1"
                     /translation="MSSSSEKGADRFSASYNGLLFPPLSGLKRAVAVFILITLAAALT
                     VPAGCRRPFTSNTDCASEIPPDSASGYIQECSDYTDPVSPWHVCITKTKGSRSKDVLF
                     YFHGYSNDQCAWLAPRRPGSNYRAAVVEWWEANGKQAPTVVVFSVGRKFLLTPDGGRY
                     PVSWFVKTVLPEIRKKLGPKRGEYIIWGTSMGGQNALQLYMRHPGLWDKAVFNSPMIP
                     VCDPYAEDGGEACIRGIATRQIPFLTMESLNLVREFYPTGEEWAEADPLRAGPRLLNS
                     GYPPMLIEIGTSDEFGFYPGADAFRKIAVEKGVSVEFIVHGVKSGLFWKVPNHLYFDA
                     GRLARFIAGD"
     gene            2669..3526
                     /gene="ppk2"
                     /locus_tag="VFJ67_01520"
     CDS             2669..3526
                     /gene="ppk2"
                     /locus_tag="VFJ67_01520"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HET7289088.1"
                     /translation="MGNKKKDGGKKNVNGTELPVIAEERPERDLDDKTYDEELKKLET
                     ELVKLQDWVKSAGKKIIVLFEGRDAAGKGGMIKRIVEPLNHRVCRVAALGVPTEKEKT
                     EWYFQRYVNYLPSAGEIVLFDRSWYNRAGVERVMGFCTDEEYTEFIRACPEFEKAVIH
                     SGAILLKYWLEISAEEQEKRFRERIDDPRKQWKISPMDMEARKRWYDYSRARDDMFKY
                     TDTDISHWYIVPADDKKRARLNCITHMLSRIPYKKVPREKFEIPSLQKIKGYKPPRVP
                     DAELIPEKF"
     gene            complement(4146..4481)
                     /locus_tag="VFJ67_01525"
     CDS             complement(4146..4481)
                     /locus_tag="VFJ67_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289089.1"
                     /translation="MKKSIIFGIAAMSLAFLFTANDSFADRDATPYEAAAVYGALKSQ
                     GCTVADDIEFEPFGGEIRYRPLASVYREDVPEYGRPGIFKTEAICSDGERYDIKLDSR
                     LNILSMDPD"
     gene            complement(4590..4931)
                     /locus_tag="VFJ67_01530"
     CDS             complement(4590..4931)
                     /locus_tag="VFJ67_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289090.1"
                     /translation="MAGMNVWEVIDMKKSIIIGIAAMSLAFLFTANDSFAYRDATSYE
                     EAAVYGALRSQGCTVTDDVEVESFVAVNRYGEPRDTMPGVYKTEAICSDGEKYDVKLD
                     SQLNILTMKLD"
     gene            complement(5135..5554)
                     /locus_tag="VFJ67_01535"
     CDS             complement(5135..5554)
                     /locus_tag="VFJ67_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289091.1"
                     /translation="MNISDTFKLPGNLKGEIARDLVALGGLPFYLLVLVRSSIGGYMS
                     FLAHIAIALPALYLSSRVLRGSNLHIARALILVVFTSIFYDALPFTVFSTLVWCGMIY
                     SLAYLKTGTREILKGIAVGAVSVILSYALTLLIVPVK"
BASE COUNT         1439 a         1498 c         1638 g         1287 t
ORIGIN      
        1 ccccataata gctcaggcga gacattacgg gggaaggatg ctcatatcca tgttttccgg
       61 tttacgggac ggtacggatg attgtgcggc tgcccggtca gtgaggcttg aagaaacgaa
      121 aagaacacac gtaataagaa aagaacacac gtaacaaaag gagaaagaaa tgcaaggtaa
      181 aaaactttac ataggcaacc tcgcctacaa cacgacggcc gaggaattaa ccgagctcct
      241 gtccaaatac gggaccgtat cggacttacg cctgataggc gacaaggggt tcggtttcgc
      301 cgagatgggc acgcagcagg aggccgagaa ggcaattagc gagctcgacg gctacgagtt
      361 caagggccgt cctctaaagg tcaatgaggc gcgtaagccc accgataagc ccagaggcgg
      421 acggagcggc ggcggaggcg ggtacggcgg aggcggcgga ggcggcggcg gttacggccg
      481 gagaaggtga gccgggcagt cccatcgggt aagcatccat tcgcgtcccc atactgatac
      541 tccccatgcc gatccccatg tcatgcggca cgggagaaaa cgggctctca cggctttgaa
      601 acgcttccgc ggcgcgccat gcgcccggat gcggcctgag cgcccgttac cgaggagctt
      661 gaaagaaagt caggcccccg ggcctcacat ccccgctctg tcatttttat tccattcccg
      721 tatttgaaaa ctcactcccc tgatttattc ttttatctcc gtgcggtaaa aattcattta
      781 cgaagaggga aatgttatgc cgaaaataga cgagattgcg ccgggcgttt accggatatc
      841 cgtaacatac gaggagatag gactacagtt caaccacttc ctcgtgaagg acgacgagcc
      901 cctgctcttt cattccggac tcaacgggat gttcaacgag gtgtatgaag gcgtatcgaa
      961 attaataaag ccctcggacc ttcgctgggt cggcttcagc catttcgaat cggacgagtg
     1021 cggctcgctc aacaggtggc tcgaagcggc tcccagggcc gaagccgtat gctcgagcct
     1081 gggagcgctc gtgagcgtga acgacttcgc caagcgcccg gccaggggaa tgaacgacgg
     1141 cgagacgctc aggaccggcg acaaggtatt caggttctgc tctacggcgc acctgcccca
     1201 cgggtgggac gcgggggtat tctacgagga gacggacaag acgctcttct gctcggacct
     1261 attcacccac ttcggcgagg tagagcccat aacggagtcc gacgtggtcg ggcgcgccgc
     1321 agattcgctc cggaacatgc agcagagccc cttcgcctat tacattcctt acacccctca
     1381 gacgaagggc atattgagca ggctcgcctc gctcgatacg aaaacactcg ccataatgca
     1441 cggctcgtcc tacaccgggg actgcgggaa ggcgctcctc gacctctctt cggtgatcga
     1501 ggaagagctc ggttgacgtt ttgagcccgc ggatacgagt tcctgccggc ggattgctga
     1561 gatggggaaa ggggggggaa tcatgtcttc gtcatcggag aaaggtgcag acagatttag
     1621 cgcgtcgtat aacgggcttt tgtttccacc gttatcgggt ctgaaacgag cggttgcggt
     1681 ttttatactc atcacccttg ccgcggcttt aactgtcccg gccggctgca ggcggccctt
     1741 tacctcgaat accgactgcg cctcggagat ccccccggat tcggcttccg gatatatcca
     1801 ggaatgctcc gattataccg acccggtatc gccctggcac gtttgtatca cgaaaacgaa
     1861 agggtctcgc tcgaaagatg tcttattcta tttccacggc tattcgaatg accagtgcgc
     1921 gtggctcgct ccccggagac ccggttcgaa ctacagggcg gcagttgttg aatggtggga
     1981 ggcaaacggc aagcaggcgc cgacggtggt ggtattcagc gtcggccgaa agtttctcct
     2041 caccccggac ggcgggcgct atccagtctc atggttcgtt aaaacggtct tgccagagat
     2101 tcggaagaaa ttgggtccga aacgcgggga atatattatt tgggggacat cgatgggagg
     2161 gcagaacgcc ctgcagctat acatgaggca tcccgggcta tgggataaag ctgtatttaa
     2221 ttctcccatg atccctgtat gcgaccccta cgctgaagac ggcggggagg catgcatacg
     2281 cggtatcgct acccggcaga ttccattcct gaccatggaa tcccttaatc tggtccggga
     2341 attctatccg actggcgaag agtgggccga ggcagacccc ctgagagccg gtccccggct
     2401 tcttaactcc gggtatccgc ccatgctcat cgagatcggg acctcggatg aattcggttt
     2461 ctatccgggt gcggacgcgt ttcggaaaat cgccgtggag aaaggcgtat cggtcgagtt
     2521 catcgtgcac ggcgtgaaga gcggtctatt ctggaaggtg cctaaccacc tgtatttcga
     2581 tgcgggcagg ttggcccgat tcatcgcggg ggattgagga cggtttataa ttagacaccg
     2641 gtacaaatat ttttcgggag cgcatacgat gggaaacaaa aagaaggacg ggggaaagaa
     2701 aaacgtaaac ggcacggagc ttccggtgat agcggaagaa aggccggaaa gggacttgga
     2761 cgataagaca tacgacgaag agctcaaaaa gctcgaaaca gagctggtga agctccagga
     2821 ctgggtgaag tccgcgggga agaagataat cgtccttttc gagggcaggg acgccgccgg
     2881 gaagggcggc atgataaagc gcatagtaga gccgctcaac caccgcgtgt gcagggtggc
     2941 cgccctcggg gtcccgactg agaaggagaa gacggagtgg tatttccagc gctacgtcaa
     3001 ctacctcccg tccgcgggcg agatagtgct cttcgacagg agctggtata acagggctgg
     3061 cgtcgagagg gtgatgggct tctgcacgga tgaggaatac acggaattca tacgcgcgtg
     3121 ccccgagttc gagaaagccg taattcactc gggggcgata ctgctcaagt actggctcga
     3181 gatttccgct gaggagcagg agaagaggtt ccgggagcgc atcgacgacc cgcggaagca
     3241 gtggaagata agcccgatgg atatggaggc gcggaagcgc tggtacgatt actcgcgggc
     3301 gagggacgat atgttcaaat atactgacac ggacatatcc cactggtaca tagtgcccgc
     3361 cgacgacaag aagcgggcga ggctcaactg catcacgcac atgctgagcc ggattcctta
     3421 caagaaggtg cccagggaga agttcgagat accgtcgctc cagaaaataa agggctacaa
     3481 acctccccgc gtcccggacg cagagctcat accggagaag ttctagatta gcttcgaagc
     3541 tgccttatgt tggagagcga atcagcctgc ttctgtctct ttaagtcttt accaaataca
     3601 ccaatccctt tgtcggaggt atctaaaaaa ataggggggc agtcacagtt tatgaccgtc
     3661 ccccggtaga gcgggttaat cttcttcttt cgtcacgata tttaagtccg gaccttttcc
     3721 cacgggaaaa aacatgggcc ctctaatcta tatttattcc gaagaataaa ctgccgctgg
     3781 cgggcggata ataatatggg acggggtagg gtgccggctc gtagtatacg acgggaggag
     3841 gaggcgcgta atatatataa cgttccggat aatagggtct gtagtagtaa ttgctgttga
     3901 cataagtacg atgataattg ttgtatccgc catgatattt gtggtggccg taatgattgc
     3961 cggccatgct cggtaacggc acggcaatga taaggcccat tattattaga aacgtaatca
     4021 atatttttct catttttttc acctcgctat gtataatgtc tcgattctaa aaccggaatg
     4081 cccgggctgc gaccgggcat tccgtatcgt tataaacccg gattttcagt ccggttaagg
     4141 acattctagt ccggatccat cgataagata ttcagccgcg aatccagttt gatgtcgtac
     4201 ctttcaccgt cgctgcagat cgcttccgtt ttaaagattc cgggtctgcc gtactcgggc
     4261 acgtcctctc tgtatacgct ggctaacggg cggtatctga tctctccgcc gaagggctcg
     4321 aattcgatgt catcagccac tgtgcacccc tgtgatttca aagcgccgta aacggcggct
     4381 gcttcgtagg gtgtcgcgtc tctgtctgca aacgagtcgt ttgccgtgaa caggaaggct
     4441 aatgacatag ctgctatacc gaaaataatc gacttcttca tgtcaatcac ctcctaatga
     4501 tgatattgtt ctcaaacatc tataccggaa ataattccgg acattcccgc attcaataaa
     4561 tagggattct gcaaccaggc gtcagcctgt taatcaagct tcatggtcaa tatattcagt
     4621 tgcgaatcca gcttgacgtc gtacttctca ccgtcgctgc atatagcctc ggttttataa
     4681 acgcccggca tcgtgtcgcg cggctcgccg tatctattca cggcgacgaa ggattcgacc
     4741 tcgacgtcat cagttacggt gcatccctgc gatcttaaag cgccgtaaac ggctgcttct
     4801 tcataagagg tcgcgtctct ataggcaaac gagtcgtttg ccgtgaacag gaatgccaat
     4861 gacatagctg ctataccgat aataatcgac ttcttcatgt ctatcacctc ccatacattc
     4921 attccggcca tcaaatcttt atcgccgcaa tgcgtgtata tttactaaca tacagggggc
     4981 aagggccgtg ccagccggcg ggaattccga tttattcaca atgattgtgg cgggttagtt
     5041 tttgctttga atttgaatgt caaaacagaa aactcgattt agtatgaaac ggaacaatcg
     5101 agcatgtgtc tatacggaca caattcaccg gagtttattt caccggaacg atgagaagcg
     5161 tgagcgcata gcttaatatc acgctcacgg cgcctaccgc tatccctttc aatatttccc
     5221 tcgtaccggt cttgagatac gcgagtgaat atatcatccc gcaccatacg agtgtagaaa
     5281 aaacggtgaa gggcagggcg tcataaaaga tcgacgtgaa tacgacgagt atgagcgccc
     5341 tcgctatgtg gaggttcgag cctctgagaa ccctcgatga caagtagagc gcggggagcg
     5401 cgattgcgat gtgggcgagg aacgacatgt aaccgcctat gctcgacctc accagcacga
     5461 ggagataaaa cggcaggccg cccagtgcga cgaggtccct cgctatctct ccctttaaat
     5521 ttcctggcaa cttaaacgtg tcgcttatat tcatctttat gccctcctta atttccccat
     5581 acaaaccatc aacgtagcag gagcggcttc cagccgcgat taatgtaaga tgtcgcgccg
     5641 ggatggtgct cctacggaaa aaagggtatt cacccctcct gacccgaata ttaaaccttg
     5701 cccggcgatg cctacggtct aataatgtta tataatggac ataggaggtg tgctatggca
     5761 agggtcaaat atatagagga agagacgacg gacaatccgg tgataaagga atcgttcgag
     5821 cgcatgcgga agaagagggg gaaggtcacc aacatttaca ag
//
LOCUS       DASSJH010000071         3686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1207076, whole genome shotgun sequence.
ACCESSION   DASSJH010000071 DASSJH010000000
VERSION     DASSJH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207076"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..528
                     /locus_tag="VFJ67_01540"
     CDS             301..528
                     /locus_tag="VFJ67_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289092.1"
                     /translation="MKKVTNVRQFAVAVGLSAAFVAAGVLGFAFVIITLILALLGFTG
                     FGGTVAGIAKIILLVLATLSLLFLVPGQRKG"
     gene            complement(699..1001)
                     /locus_tag="VFJ67_01545"
     CDS             complement(699..1001)
                     /locus_tag="VFJ67_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7289093.1"
                     /translation="MAGITVITLIALTTFDKVTHAIHCPAVYFVIISLEGNFLTPMIL
                     GKRLLLNPVVIFLAIIFWGWIWGVPGALIAVPVVVIVKIVCDKIESLAAVGEILGK"
     gene            complement(947..1813)
                     /locus_tag="VFJ67_01550"
                     /pseudo
     CDS             complement(947..1813)
                     /locus_tag="VFJ67_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            complement(1931..3307)
                     /locus_tag="VFJ67_01555"
     CDS             complement(1931..3307)
                     /locus_tag="VFJ67_01555"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7289094.1"
                     /translation="MPRTILLIDDEIFFTKKLAKNLSDVGYDVFTAFNAAEGIELAKK
                     CLPRIAIVDLRLPDMDGIELIKRLNELDPPPIPIIITAHAYVEAAVSAMKLGAYDFLE
                     KPFPVDKLKIALQNALKSANLEKAVDVTTRQERKRYGFDSLVGTSGAIREVASLLEKL
                     SACDPKMVLITGETGTGKGLAAKVLHYNGKRAEKPFLEMNCAAIPENLLESELFGYEA
                     GSFTDAKKARSGILEDADGGTILLDEIGDMSLNLQAKLVKVVEERKFRRLGGKKDIKV
                     DLQIVAATNKDLAEEVNDSRFRQDLYHRLNVISFEMPTLRERKEDIPPITKHFVEFFN
                     TDINRNIKTIPDDILKSLVYYDWPGNVRELRSTIEKAMILSEGEVLNSEYVRINGHDR
                     EVSVRTDGNKMTIDISLDDSSLEEIEQKIIKKTLEDNEWNQTRTARILKMNRQNLRYR
                     MKKMGLLN"
     gene            complement(3323..>3686)
                     /locus_tag="VFJ67_01560"
     CDS             complement(3323..>3686)
                     /locus_tag="VFJ67_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7289095.1"
                     /translation="AREISLDFEMIQQVIMNLVLNSIESITGPGGRIIIKSYYANNLY
                     IIEISDNGSGIRESHLNMIFEPFFSTKNNGTGLGLAISKKIINLHNGSIRVESANRSG
                     TMFIISLPTDLPDITKTT"
BASE COUNT          802 a          898 c          905 g         1081 t
ORIGIN      
        1 ggggccactg tcatatgctt cgatgaatcc gaggcggctt cggcgctggc ggacgctctg
       61 ctccaggata tcaggcaacg aaacgaataa gctgcccctg cgcggttcta ttctgtgtgc
      121 tgcggtgaat gtgtgaatac attaagtgtt tggatttgtt cagtctccgc tgtcccgagc
      181 ctgacaatct gagcggcaaa tgttgcatgt aaggccgtga ctttgttcca actcccccta
      241 gggaatgaat tttctcttct taagatctcc tggcatatat gttgcgtaaa tataattact
      301 atgaaaaaag taacgaacgt gaggcagttc gcggtagcgg tcggtttatc agcggctttc
      361 gtggcggcgg gcgtgctggg attcgccttc gttataatta cgctcattct cgcgttgctg
      421 ggctttactg gattcggggg aactgtcgcc ggtatagcga agattatctt gctcgtattg
      481 gctacgttat ccctgctgtt tctggttccc ggacaacgga aaggatgatc ggacttgcac
      541 gctgattgga gtttcccgta aagatattgt tccctctatc aggaccacga gtgatctcgg
      601 agaatccact cgtacccgct atagacatgc tgctgcatgc ttttcgatct gaccgttcat
      661 cgcgattgct cgtatgaaaa acatccgcga gtcaacgctc acttacccag gatctctcct
      721 acggctgcga gagactctat cttgtcgcag actatcttca ctatgaccac tacagggacg
      781 gctatcaggg cgcccggtac gccccatatc catccccaga atattatcgc gaggaatatg
      841 acgacggggt tcagcaagag acgcttcccg aggatcattg gggttagaaa attcccctcg
      901 agagaaataa tcacgaaata tacggccgga caatgaatcg catgggttac cttgtcgaac
      961 gtcgtgaggg cgatgagggt gatgacagtg atccccgcca cacctggccg agatagggaa
     1021 taaaattgac taccgcagcc attactcccc acaggaaagg gttcggcatg ccgagcagat
     1081 gcatcgcgaa tcctatgacg attccgagac atatgtttat gagcgttatg gtgtaaaggt
     1141 aggtggatat gtcgcggtta atgtgcctca ttatctctac tgcctgtttt ttattctgca
     1201 gcgtaggcag tacgtttatc actttccgaa agaacatatc gccggatgcc aggaggaaat
     1261 agaggagtat cacggtcgtg gccaggccga agaccagctc gtaggtttaa taaaagagcg
     1321 tgttcaggag cagctgcttt tttacttgta cggacttctc gccatcctta gcgttcattt
     1381 cggttatctt ctctacactc ttagtggctt cgctcacttt ctcgacctgc tcctttataa
     1441 agcggagctt acgctcaatc tgtttgaatg tggcgggggc tttgtccacc cattccgatg
     1501 cgggctgata gtagacatag aacgatacgg taatgatcgc tatgagcagg gaaagcacta
     1561 ttgcggctcc gaaagcaggc ggtatatata cttttcgcaa ggtatctaca gcgggagaaa
     1621 gcatgagatt gaacagcaat gcgaaaaaca ggggaatgaa aagggcccgg gcaaaataga
     1681 acgtgtacat cactgcaaga atgaacatac ccgtcagggc aatagaacgg atgttaagct
     1741 gtctgttgaa taactctttg agactcgata cctcgggtgt ttctgcggag gtgttttctt
     1801 tttcgtttat cattgcggtc ggcggattat tacttggtta tgatggatgt tgatcgtttc
     1861 cgttaatttt gtcattccgt ggagttttat ggttggtcct ccgaatatcg tccctcgccg
     1921 actgcgctac tcagttaagc aggcccatct ttttcatgcg gtatctgagg ttctgcctgt
     1981 tcattttgag tatcctggct gtcctcgtct gattccattc gttgtcttcg agagtttttt
     2041 tgataatttt ttgctctatc tcctcgagcg aggaatcatc aagggatata tcgatggtca
     2101 ttttgtttcc gtcggtcctc acgctgactt ccctatcgtg tccgtttatc cttacgtact
     2161 cggagttcag cacttcgcct tcgctcagga tcatcgcttt ttcgatcgtg ctcctcagct
     2221 cccgcacatt gccgggccag tcgtaataca caagcgactt gagtatgtcg tcagggatcg
     2281 tttttatatt ccgatttata tcagtgttga aaaattccac aaagtgtttc gttatcggcg
     2341 gtatgtcttc tttacgttct ctgagcgtcg gcatctcgaa gcttataacg ttgagcctgt
     2401 ggtaaaggtc ttgtctgaag cggctatcgt ttacctcttc agccaggtct ttattcgtag
     2461 cggcgacgat ctgaagatcg acctttatgt cctttttacc gccgagccgt ctgaattttc
     2521 tttcttccac cactttcacg agctttgcct gaaggttgag actcatatcc ccgatctcat
     2581 cgaggagtat ggttccgcca tcagcgtcct cgagtattcc ggacctcgct ttcttcgcat
     2641 ccgtgaaaga gcccgcctcg tagccgaaga gctcactttc gagaaggttc tccggtatgg
     2701 ccgcgcagtt catctcgagg aacggcttct ccgccctctt gccgttataa tggagtacct
     2761 tcgccgcaag cccttttccg gtcccggttt ccccggtgat caagaccatc tttgggtcgc
     2821 atgcagagag cttttccaga agtgaggcga cctctcttat cgctccgctg gtcccgacga
     2881 gtgagtcgaa tccgtatctt ttccgctcct gtctcgttgt cacatcgacc gctttctcaa
     2941 ggttggctga ctttagagcg ttttgcaggg cgatctttag cttatcgacg gggaacggtt
     3001 tttcgaggaa atcgtaggcg ccgagcttca tagccgatac ggcggcttcc acataagcgt
     3061 gcgcggtaat tatgatgggg atcgggggtg ggtcaagctc gttcagtcgt ttgatgagct
     3121 ctatgccgtc catgtccggt agcctgaggt caacaatggc gatcctcggc aagcactttt
     3181 ttgcaagctc aatcccttca gccgcgttga acgcggtaaa cacgtcatac ccgacgtcgg
     3241 acagattctt cgcaagcttc ttggtaaaga atatttcgtc gtctatcaat aatattgttc
     3301 tcggcatttt ccattccctc ttttaggttg tttttgtaat atcaggaagg tcggtcggca
     3361 ggctgataat gaacatcgtt cctgatctat tcgcactctc aaccctgata cttccattat
     3421 gcaaattaat aatctttttt gagatggcca gaccgaggcc cgtcccattg tttttcgtgg
     3481 aaaaaaacgg ctcgaatatc atgttcaaat gactttcgcg tatccccgat ccattgtcgg
     3541 aaatttctat tatgtacagg ttattcgcgt aatatgattt gataattatc cttcccccgg
     3601 gtccggtaat agactcgatc gagttaagca caaggttcat gattacctgc tgtatcattt
     3661 cgaaatcaag gcttatttct ctcgcg
//
LOCUS       DASSJH010000072         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1316662, whole genome shotgun sequence.
ACCESSION   DASSJH010000072 DASSJH010000000
VERSION     DASSJH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316662"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VFJ67_01565"
     CDS             <1..1123
                     /locus_tag="VFJ67_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET7289096.1"
                     /translation="SGKYGTLRPATIKTGEGEGVFEGRVDFRDLSYTGKGKMSGMLMM
                     TYPADVTANLDGDIEVEGKFLNAFIKGNVKANNIQIMVPDKPLKEIESIKFIDKQDSD
                     KEEFIFTGEKKEDFVEEFIALDLDVDIPRDSWVKGGGANIEVEGKLEINKSYKEPYLI
                     TGSIDVIRGDYQFMGRLFKVDNGTVSFRGKKIVDPFLDLRATYEVSSVEVYINITGTA
                     EKPKIQLSSDPPLDEDEIVSYLVFGTSSENLGTDQRVEFQEKAGEVLGTMAVGELRNT
                     LGEELAIDVMTIKGGQTGFRDTHLEIGKYLTDDLYIGYERYSYERYFYERYFFSPGLP
                     SSTVTANRAVIEYRVFDFLTLESEIGEEAGADVFFNFDY"
     gene            complement(1180..2367)
                     /gene="alaC"
                     /locus_tag="VFJ67_01570"
     CDS             complement(1180..2367)
                     /gene="alaC"
                     /locus_tag="VFJ67_01570"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HET7289097.1"
                     /translation="MQHIPEEFSLIRRLPPYVFSIVNELKSRARARGDDIIDLGMGNP
                     DQATPDHIVKKLIEAAKDPRAHRYSASAGIPKLRKAVSDWYKKNYDVELDPDTEVVTT
                     IGSKEGICHLMLSILSPGDTVIVPNPSYPIHIYSVIISRGDVHSVPFTRDSNILDGIE
                     KAVKETWPKPKVLMISFPNNPTTAVVDLGFFKDIYELAKEAGLIVVHDLAYAELCFDG
                     YKAPSFLQVPGAQDIGVEFYSLSKSYNMPGWRVGFMVGNARIVSALKRIKSYMDYGIF
                     TPIQVASIAALNGPQDCVEDIRKLYKSRRDKLVDGLSKAGWKIPKPKGTMFVWAEIPD
                     EFKAMGSLEFSKLLIEEAKVAVSPGIGFGNYGEGNLRFALVENEKRIMQAVRGIRKFL
                     NSY"
     gene            2463..2861
                     /locus_tag="VFJ67_01575"
     CDS             2463..2861
                     /locus_tag="VFJ67_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016976221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET7289098.1"
                     /translation="MKKNIGLFSALLLTVFAVPLVCAQEVRQYGNASWYASSLHGRKT
                     ASGECYDKDEFTGAHRDLPFGTLVKVKNLYNGKEVIVRVNDRGPFIKSRIIDLSRAAA
                     ITLGILNMGTARVSLEVISLPVSVDPGSAS"
BASE COUNT          688 a          725 c          813 g          675 t
ORIGIN      
        1 gtcgggcaaa tacgggaccc tgcgccccgc taccattaaa acgggagagg gcgaaggggt
       61 attcgaaggc agagtcgact tccgcgacct ttcttacacc ggcaagggca agatgtcggg
      121 aatgctcatg atgacctatc ccgcggacgt caccgccaac cttgacgggg acatcgaggt
      181 ggaggggaag ttcctgaacg cgttcataaa ggggaacgtc aaggccaaca atatacagat
      241 catggttccg gataagcctc ttaaagaaat agagagcata aagttcatag acaagcagga
      301 ctccgataag gaagagttca tattcacagg cgagaagaaa gaggatttcg tcgaagagtt
      361 catagcgctc gacctcgacg tggacatccc cagggattcg tgggtgaagg ggggcggcgc
      421 gaacatcgag gtcgaaggga agctcgagat taacaagagc tacaaggaac cctatctgat
      481 cacggggagt atcgacgtca taaggggcga ttaccagttc atgggcaggc ttttcaaggt
      541 cgataacggc accgtgagct tcaggggcaa gaagatcgtc gatccgttcc tcgacctgcg
      601 ggcgacgtac gaggtatcga gcgtggaggt gtacataaac atcaccggca cggcggagaa
      661 accaaagatc cagctctcta gcgatcctcc gctcgacgag gacgagatcg tttcgtatct
      721 cgtattcggg acgtcttccg agaatctcgg tacggaccag agggtcgagt ttcaggaaaa
      781 agcgggagaa gtgctgggca ctatggctgt cggagagctc aggaatacgc tcggggaaga
      841 gctggcaatc gacgttatga ccataaaagg cggccagacc gggttcaggg acacacacct
      901 cgagatcggc aaatatctga cggacgacct gtatatagga tacgagagat actcatatga
      961 aaggtatttc tatgagcgtt atttcttcag cccggggctg ccgtcgtcca cggtcacggc
     1021 gaacagggcg gtcatagaat acagggtctt cgatttcctt acgctcgaga gcgaaatagg
     1081 agaagaggcc ggggccgacg tcttcttcaa tttcgactac taggaatatt ctcccgtgtc
     1141 atgcatggca agcgcgggtg cggtaatcag cggcgggcat cagtagctgt tgaggaattt
     1201 ccttatgccc cggacggcct gcattatcct cttctcgttt tcgacgagcg cgaatcttag
     1261 attaccctcg ccgtaatttc cgaaccctat accgggagag acagcgacct tggcctcttc
     1321 aatcaggagc ttcgagaact cgagtgatcc catggccttg aactcgtccg gtatctcggc
     1381 ccacacgaac atggtcccct ttggcttcgg tatcttccat cccgccttag agaggccgtc
     1441 cacgagcttg tcccttctcg acttgtagag ttttctgata tcctcgacgc agtcctgcgg
     1501 gccgttgagc gccgcgatcg aggccacctg tatgggagtg aatatgccgt agtccatgta
     1561 actcttgatg cgcttgagcg cggacactat cctcgcgtta cccaccatga agcccacgcg
     1621 ccatcccggc atgttgtagc tcttcgagag ggagtagaat tcgacgccta tgtcctgagc
     1681 tccgggcact tgcagaaagc tcggggcctt gtatccgtcg aagcagagct cggcgtatgc
     1741 cagatcgtgc actactataa gacccgcttc tttcgcaagc tcgtatatat ccttgaaaaa
     1801 accgaggtcg accactgcag tggtcgggtt gttagggaaa gagatcatga gcaccttagg
     1861 cttgggccag gtctccttga ccgccttctc tatgccgtcg agtatgttcg agtctctcgt
     1921 gaaagggacg ctgtgcacgt cgcccctcga tataattacc gaatatatgt gtatcgggta
     1981 tgaagggtta ggcactataa ccgtgtcacc cggggaaagt atggagagca tgaggtggca
     2041 tatgccttcc tttgacccga tcgtcgtaac gacttcagta tcggggtcga gctcgacgtc
     2101 ataattcttt ttataccagt cggatacggc cttcctcagc ttcggtatgc cggccgaggc
     2161 ggaatacctg tgagccctgg ggtccttggc tgcctctatc aattttttca cgatatggtc
     2221 gggagtcgcc tggtccgggt tacccatacc caggtcgatt atatcgtccc cgcgggccct
     2281 cgctctcgac ttaagctcgt ttacgatact gaaaacatag ggcggaagcc ttctgattaa
     2341 agagaattct tcaggtatat gctgcattca gggtcctttt tcattttcat ttaattttat
     2401 tcgaatcctt gacaatctca agaaaaataa gcgagaatag taaaaaacct caatgaaggg
     2461 tcatgaagaa aaatatagga ttattttcag ctttgctgct tacggtgttt gctgtccctt
     2521 tggtttgcgc tcaggaggtc cgtcagtatg gtaatgcttc ctggtacgcg agttctctcc
     2581 acggcaggaa aacggcgagc ggcgaatgtt atgacaagga cgaatttacg ggcgcccaca
     2641 gggatctccc cttcgggacg ctggttaagg tgaagaacct gtataacggt aaagaggtaa
     2701 tagtgagggt gaatgacaga gggccgttta tcaagagtcg tataatcgac ctctcgcggg
     2761 ctgcggctat tacgctcggg attctgaata tgggcacggc gagggtaagc ctcgaggtga
     2821 tatcgctgcc cgtaagcgtc gatcccggat ccgcttcgta agccgcatca tacggcttcc
     2881 cgtcctcggt catcagttat c
//
LOCUS       DASSJH010000073         4861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1424906, whole genome shotgun sequence.
ACCESSION   DASSJH010000073 DASSJH010000000
VERSION     DASSJH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424906"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFJ67_01580"
     CDS             <1..232
                     /locus_tag="VFJ67_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289099.1"
                     /translation="IEKDTLRYNRVLKASGSVKKEIPCPDAGKWEKEGKAVLYVEKMT
                     LNLRTMIETHSDDFFCEPAPAITKRASDYTIH"
     gene            complement(865..1053)
                     /locus_tag="VFJ67_01585"
     CDS             complement(865..1053)
                     /locus_tag="VFJ67_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289100.1"
                     /translation="MRSETKVTLERAARILGTDVTTLLDEIYEVGITIHNDRGSGFDY
                     ISASDFENLKESMGFADH"
     gene            complement(1302..1832)
                     /locus_tag="VFJ67_01590"
     CDS             complement(1302..1832)
                     /locus_tag="VFJ67_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7289101.1"
                     /translation="MRSISGWSFDLSRIIVSFLIVTIAVAGFLTVQTVRSYAQPATCN
                     VAVFKRATPSDGTLFTFDVTGATNTAGFDLVDGDDGGVVISAGSTAQVVEEVPVGWRL
                     RDVECETNGVFVSDIENGIELFCSPNGGNAECFFFDINVDNVPTLSEWGMIAAAVGLG
                     FVGVFFAVRRRKAFNS"
     gene            complement(1911..2741)
                     /locus_tag="VFJ67_01595"
     CDS             complement(1911..2741)
                     /locus_tag="VFJ67_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289102.1"
                     /translation="MGEGFEHQRSPTSGGELIYSEGTHDLCCSRNPYESTAAGAEEAG
                     GALHEERAEYGDEKNSHIYKVWGEEEIGRLRQSAGGSASADMRYSLAPAHHIPVKSDA
                     RERSAADRTVETSAGEPDAAASSATPVKSYAEEKVIGPAGEVPSADTDSAHTPDTPVK
                     THKCVFAVSMTESARFGTLWSVRVVHGETNYFALMRLMQKIEKRRASGEPLAANAPHQ
                     PKRRERSAAARLAFRYGIEEEDVYSIKFSSETVKRLAVFYGIPERDVRRIREEEVEEE
                     "
     gene            complement(3071..3406)
                     /locus_tag="VFJ67_01600"
     CDS             complement(3071..3406)
                     /locus_tag="VFJ67_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HET7289103.1"
                     /translation="MIRNIRAAAFAAAVMALLIAFGAAGCDALKRRGEGKACGGKSGI
                     KCAGELRCDVEAGRCGEEGLEGVCVNLETICDKKFKPVCGCDGRTYLNDCNRVFSNVR
                     KAHDGKCEK"
     gene            3922..>4861
                     /locus_tag="VFJ67_01605"
     CDS             3922..>4861
                     /locus_tag="VFJ67_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET7289104.1"
                     /translation="MFPKRMEMKSGGTGKTGVFAGFIKGQLKVLRECKPYNLICLLIV
                     LSIFALPIVSWAGDPGGGATGGINDVPAATPGSPTLLEVAAQVGHNKISINIVWTLIT
                     GFLVMFMQAGFAMVETGFTRAKNVAHTMGMNFMVYAIGMLGFWICGFAFMFGGIGALG
                     TLGGVTPLDHEVTVTLFGHTFGLLGMKGFFLSGDAYDAGVFTMFLFQMVFMDTTATIP
                     TGSMAERWKFLPFVIYGFFISMIVYPLFGNWVWGGGWLAQLGVNFGLGHGHVDFAGSS
                     VVHMVGGVAALAGAIVLGPRIGKFKKDGTALPIPGHN"
BASE COUNT         1043 a         1461 c         1196 g         1161 t
ORIGIN      
        1 gatagagaaa gacacgctcc gctacaaccg cgtgctcaag gcgagcggct cggtgaagaa
       61 ggagatacca tgccccgacg ccgggaaatg ggagaaagaa gggaaggcgg tcctgtacgt
      121 cgagaaaatg acactcaacc tccggaccat gatcgagacc cactcggacg atttcttctg
      181 cgagcccgcc cccgcgatca cgaaacgggc ctccgactac acgatccact gacacacaca
      241 atgtgtgctc ataatcgatt ctctccagcg cgttcccggt caaaccaact tccccgacaa
      301 ttttctcttt cgcagttaat tccctccttt gtaaaggagg gttagggagg atttggtttt
      361 tacccgtccg tgctgcttgc ggcacgtcgc ccgtaggcta tgagccgtag ggtgatccgg
      421 cgtagccctg gcgtagccgg gagcttttcg aactatgaac gggttccacc atacatgccc
      481 gcgacgactc tgccgttcac cattaacgtt aattccttcc ccctaaaaag gggggaaggt
      541 gaggatgggg gtgttacctt tcactcatcc atccaacaaa ccccgttcgt ccttcattta
      601 tttccctccc cctagaggga cggttccgag cgtgcgcagg aactggtcgg atctacaacg
      661 aagctgatgc gtccaatcga cttcagcctc gatgccgcct aaaccaaatt agggccaggg
      721 tgggggtgaa cattacacta atccacccaa caaaccccta tcatctcgtg caggagcctg
      781 tcccgagtga gttcactcgt ctcagctaat gcccgaagca tgaacgactc gccgccccgc
      841 gcctctgcgg cgagcccgca gccctcagtg gtcggcgaag cccatactct ctttcaggtt
      901 ctcgaaatcg gacgcgctta tgtagtcgaa tcccgagccc ctgtcgttat gtatggttat
      961 gcccacctcg tatatctcgt cgaggagagt cgtgacgtcc gtgcctagaa tccttgcagc
     1021 tctttcgagt gttacttttg tttcgcttct cataacatga aagtctactg aaggatcacg
     1081 gatttggaat atcgaatttg cggtcttaac ccgtcgttca cagaaaaata attcgctatt
     1141 tatttcccaa aaaaaagccc cttcccaccg tgcggtgaga agaggccatt tattttctgt
     1201 cattgccgca gcacgtcccg cgccgccgca accatccccc attcatcttt ctttttcccc
     1261 ctttggaaaa gggggattca gggggatttg tattctatct tttaagaatt aaacgccttt
     1321 ctacgcctca ccgcaaagaa cacgccgaca aatcccaacc caacagcagc cgcgatcatc
     1381 ccccactcgc tcagcgtggg cacgttgtca acatttatgt cgaagaaaaa acattcagcg
     1441 tttccgccgt tgggggaaca gaaaagctct atgccgttct cgatgtccga cacaaagact
     1501 ccgttagtct cacactccac gtcccgaagc ctccacccga cgggcacttc ctcgactacc
     1561 tgagccgtgc tgcccgccga aattacgact cctccatcgt caccgtcgac cagatcgaat
     1621 ccggccgtat ttgttgcccc cgtaacgtcg aaggtgaaga gagtaccgtc cgacggggtc
     1681 gcccgtttga aaaccgctac attacaagtc gcgggctggg catagctcct caccgtctgc
     1741 acagtcaaaa acccggcgac agcgattgta actataagga aagaaacgat aatacgcgat
     1801 aggtcaaagg accagccgga tatgcttctc atcaaatcct ccttaaatta aattgttacc
     1861 attatataat tttctaaagt atcatgcaac tacaacataa attctccccc tcactcctcc
     1921 tccacttcct cctcgcgtat cctcctcacg tccctctccg gtatgccgta gaacaccgcg
     1981 agacgcttaa cggtttccga gctgaatttg atcgagtaca cgtcctcctc ctcgatcccg
     2041 tagcggaacg cgagcctggc cgccgccgac ctctcccgcc tcttcggctg gtgcggcgcg
     2101 ttcgccgcga gcggctcccc tgacgcgcgc ctcttctcta tcttctgcat gagccgcatg
     2161 agcgcgaaat agttcgtctc cccgtgcacg accctcacgc tccagagcgt gccgaaccgg
     2221 gcgctctccg tcatcgacac cgcaaacaca cacttgtgcg tttttacggg tgtgtccgga
     2281 gtatgcgcgc tgtccgtgtc tgcggagggt acttcacccg caggaccgat taccttctct
     2341 tcagcgtagg attttacggg tgtagcggag gaagccgctg catccggctc accggcggaa
     2401 gtctcaaccg tacggtccgc agccgacctt tcccgtgcgt ctgattttac gggaatgtga
     2461 tgggcagggg cgagagagta tctcatgtca gcagacgcgg agcctcctgc actttgcctg
     2521 agcctgccaa tctcttcctc cccccatacc ttatatatgt gagagttctt ttcatccccg
     2581 tactccgccc gctcctcgtg cagcgcgccc cccgcctcct ccgcgccggc ggccgtcgac
     2641 tcgtacgggt tcctcgagca gcacaggtcg tgcgtccctt ccgaatatat cagctcgccc
     2701 ccgctcgtcg ggctgcgctg gtgttcgaat ccctctccca tctctcgcct cctccgctct
     2761 gattttcatc tgtcttacct gtcttttgct actttaggac tattgtccat cgcttaggca
     2821 ccaatctttt ttccttcccg acattacaga atttgcgtta agaaaataga gtattaaggc
     2881 gtgaagatcg ttcaggggtg tgggttgaat tgaacgatct tagccttaat gcgagatttt
     2941 tagcaaatta tgttctgtcg gttgattttt gcttcttttc atcaaggaaa agaaggataa
     3001 aaacaactgc ctatattctt tcctttatac agcgaataac tatcccctaa accggcagag
     3061 tctgcctctc ctacttttcg cacttcccgt cgtgagcctt ccgcacgttc gagaaaaccc
     3121 tgttgcagtc gttcaggtaa gtcctgccgt cgcacccgca gacgggcttg aatttcttat
     3181 cgcagatcgt ttccaggttg acgcacacgc cttccaaacc ttcctcgccg cacctgccgg
     3241 cctcgacgtc gcacctgagc tcacccgcgc acttgattcc cgacttcccc ccgcacgcct
     3301 ttccctcacc gcgcctcttg agcgcgtcgc acccggccgc gccgaacgct atcaaaagcg
     3361 ccatgaccgc cgccgcaaac gccgccgcgc gtatattcct gatcatattt tcccgctcct
     3421 gccggggaaa aacgcagcgc ccgctgagct ccgcacggcg ggccgtcccc ttacccgtga
     3481 tgaatttaca aaacgaataa taaacctttc gatggtccgg agcaagccgc acaccccgcc
     3541 ggagccggga cggtaaacgc ttaaaattag agcttgcctg cttaattgtt gtgcagtgca
     3601 accgccgtat cggagaaaaa atatcgccaa aattcgatcc ggcggtcatg cgcgaccggt
     3661 ccggaaataa tattattttg ttttcccgac gcgcaataat ccgcgcttga aattaacggg
     3721 ttccagccgc aatcaaagaa atgaagactt acggaaggtt ataagagaga ttaaaaatta
     3781 tatggccggt gataattttt ccggcagact ccgtacgtcc gcatttgccc ggccccggaa
     3841 atttttatat ccgaacctca agcctctggc atagcttttg catttttctc tttaattctc
     3901 aacaaatgga ggccactgca catgtttcca aaaaggatgg aaatgaagag tggtgggaca
     3961 ggcaagacgg gggtctttgc cggtttcatc aaagggcaac taaaggtgtt gagagaatgt
     4021 aagccgtata atctgatttg tctcctgata gttttgtcca tttttgcgtt gccaattgta
     4081 agctgggcag gcgacccggg cgggggcgcc acgggaggga ttaacgacgt gcccgcggca
     4141 accccgggaa gcccgactct tttggaagtc gcggcacaag tcggtcacaa caagatttcg
     4201 attaatattg tgtggactct cattacgggg ttcctcgtca tgttcatgca ggccggattc
     4261 gcgatggtcg agaccggctt cacaagggca aagaacgtcg cccatacgat gggcatgaac
     4321 ttcatggttt acgccatcgg tatgctgggt ttctggattt gcggtttcgc attcatgttc
     4381 ggtgggatcg gcgcgctggg cacgctcgga ggcgtaacgc cgctcgacca cgaagtcacc
     4441 gtcacgctct tcggccatac tttcggcctc ctgggcatga aagggttctt cctgtcgggt
     4501 gacgcgtatg acgcgggcgt attcacgatg ttcctcttcc agatggtgtt catggacacg
     4561 acagccacga taccgacggg atccatggct gaaaggtgga agttcctgcc cttcgtcata
     4621 tacgggttct tcatctccat gatcgtctat ccgctcttcg gcaactgggt atggggcggg
     4681 ggctggctcg cgcagctcgg agttaatttc ggtctcggcc acgggcacgt ggacttcgcc
     4741 ggatcgtcgg tcgtgcacat ggtcggcgga gtggcggctc tcgcgggagc gatagtcctc
     4801 ggaccgcgta tcgggaaatt caagaaggac gggaccgcgc tcccgattcc cggacacaac
     4861 a
//
LOCUS       DASSJH010000074         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_557957, whole genome shotgun sequence.
ACCESSION   DASSJH010000074 DASSJH010000000
VERSION     DASSJH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557957"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /locus_tag="VFJ67_01610"
     CDS             complement(<1..1682)
                     /locus_tag="VFJ67_01610"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET7289105.1"
                     /translation="MEYPGGSKKVSTRPSKGQKPDIETAGVKPATSRETVIPAEGYDR
                     REFLKNVGKLTATGIAVGSIGLTPLLQGCNDSADGQEFDCEIGPQTGSARADTAFQVR
                     VDAAEAERGLGIPDHPCNGDESVYPNKIGNFTKGLPHNSIGEVDIDSYDALIGAIATG
                     KFEDFELLAVNGHLGCPDPSRRRRLVNPQAGYAFDLEGSDSHQLSMRAAPAFDSAEEA
                     GEMAELYWMALLRDVNFLDYNSNPISQAAAEDLSGFSDFRGPKESGIVTTGTLFRDKY
                     PGCTEGPYISQFLLKDCPFGSQFVDQRIQTTTAVDFLTDFGSWLDVQNGCQPLLSQEP
                     GGLVFCRNGRDLSQYVHIDALYQAYFVACLILLSNGVPLNIGNPYGQTPDPGSGRPLP
                     PGVSGALAQVGFGTFGGPHILALLTEVSTRALKAVWYQKWLVHRRLRPEEFGGRVEVV
                     RLGQADYPINGELFDSSVLGAVSDRTGNYLLPVAFPEGSPMHTSYGSGHSTVAGACVT
                     ILKAWFDESTIIQNPVVPSGDGITLVPYEGPALTVGGELNKLASNVSQGRNIA"
     gene            complement(1912..3036)
                     /locus_tag="VFJ67_01615"
     CDS             complement(1912..3036)
                     /locus_tag="VFJ67_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289106.1"
                     /translation="MMNCKIIRYDPLYNDQICELEKEIWTPDPAVNKSYLQWKYLDNP
                     YAALPKIYLALREGKVVAVRCLNESKWQLGNNGDGFTALCPGDTVIHPDHRNNAVYLE
                     LTKFLMNDLYELGYRYLFSFSAGPTTLLYSLAIGWKSIGRIRTMSKDSDPRTAVRKIM
                     SNHKMSKLIKNTKAAEFLRRAVNKFGRRKSLDYHKKLPAHIRVEENPVPGEMAALAGK
                     LVKENKLVLTRDEKFYKWRYGNPFSKYVFLYWYDKEMKGCLVAQTSKFKNDYMENFNI
                     IELEGVNSEIRMDLLKSAVSLLDSRSITIWSNMLDENCRKLLALNGFIESKSTSSAAE
                     HTPTVLVTVTRGPAKVIEYQGTDLLDINNWDLKMLYSDAL"
BASE COUNT          693 a          839 c          869 g          872 t
ORIGIN      
        1 gctatgttcc gcccctgtga gacgttggaa gcgagcttgt tcagctctcc cccgaccgtg
       61 agagccggcc cttcgtaagg gaccaatgtt atcccatccc cgcttggtac cacgggattc
      121 tgaatgatgg tcgattcgtc gaaccaggct ttcagtatcg taacgcaagc gccggccacg
      181 gtggagtgtc ccgagccgta agaggtgtgc atcggtgacc cttcggggaa ggcgacggga
      241 agcaggtaat tgccggttct gtcggacact gcgccaagca cggatgagtc gaaaagctcc
      301 ccgtttatcg gatagtcggc ttgtccgaga cgcactacct cgacccgtcc gccgaactcc
      361 tcgggcctga gccgtcggtg aacgagccac ttctggtacc agacggcttt aagcgcccgc
      421 gttgacacct ctgtcaataa agccagaatg tgcgggccgc cgaacgtgcc gaatcccacc
      481 tgagcgagcg ctccgctcac gccgggaggg agaggcctgc ccgagccggg atcgggggtt
      541 tgtccgtacg gattgccgat gttaagtggt acgccgttag aaagcagtat caggcacgcg
      601 acgaaataag cctgataaag cgcgtcgatg tggacgtatt gagataggtc acggccgttc
      661 ctgcagaaaa cgaggccgcc aggctcctgc gataacaggg gctggcagcc gttctgtacg
      721 tcgagccacg aaccgaagtc ggtaaggaag tctacggcag tcgtcgtctg tatgcgctgg
      781 tcgacgaact gggagccgaa ggggcaatcc ttgagaagga actgggagat gtacgggcct
      841 tcggtgcagc ccgggtattt gtccctgaag agggtcccgg tcgtgactat accgctctct
      901 ttcggcccgc ggaagtcgga gaaaccggat aagtcctcag ctgccgcctg ggagatggga
      961 ttactgttat agtcgaggaa gttcacgtcg cgcaggagcg ccatccagta gagctccgcc
     1021 atttccccgg cttcctcggc gctgtcgaat gcaggcgcag cgcgcatgga aagctggtgc
     1081 gagtccgagc cctcgagatc gaacgcgtat ccggcctggg ggttgaccaa ccggcgccgg
     1141 cgcgaagggt cgggacatcc gaggtgaccg tttaccgcga gcagctcgaa gtcctcgaat
     1201 ttccccgttg cgattgcgcc gatgagggca tcgtacgaat cgatgtccac ttcgcctata
     1261 gagttgtgcg gaagtccctt cgtgaaattc ccgatcttgt tggggtagac tgattcatcc
     1321 ccgttacatg gatgatcggg gatgcctagt cccctctcgg cctcagccgc atcaaccctg
     1381 acctgaaaag cggtgtcggc ccgcgcggaa cctgtttgcg gccctatttc gcagtcgaac
     1441 tcctggccgt cagcggaatc gttgcacccc tgaaggagcg gcgttaagcc gatactgcct
     1501 acggcgatcc ccgtcgccgt taacttgccg acgtttttaa ggaattcccg tctgtcatac
     1561 ccttcggcgg gaatgacggt ttcacggcta gtggccggtt ttaccccggc cgtttctatg
     1621 tcgggtttct gtcctttgga aggcctcgtc gagacctttt ttgaaccccc tggatattcc
     1681 ataactcccc ctccttggct aacttactat ctcttatcat aaatacatat ttttacattt
     1741 gctacttcgc ttgtaaaaat cggcctttat gctgataccc ctaattataa gccttattaa
     1801 cgtttccgag ctcactggaa acatggattt ctggctttgt ataaggggga aaaggactga
     1861 tattctaggg tataatcaat gaagcatgag cggatgagga taacgggggc atcatagggc
     1921 gtccgagtac agcattttca ggtcccagtt gtttatatca agcaggtctg tgccttggta
     1981 ttctattacc ttagcggggc ccctcgtcac cgttaccaga acggtaggcg tatgctctgc
     2041 ggcgctgctt gtcgatttgg attcaatgaa cccgtttagc gccagaagct ttcggcaatt
     2101 ctcatcaagc atatttgacc atatggttat tgaccttgaa tccagcaacg agaccgcgga
     2161 tttcagcagg tccattctta tttcggaatt taccccttct agttcaatga tattgaaatt
     2221 ttccatataa tcgtttttaa atttggaggt ctgcgctacg agacagcctt tcatctcttt
     2281 atcgtaccaa taaaggaaaa cgtattttga aaacgggttg ccgtatctcc atttgtagaa
     2341 cttttcgtct cttgtcagaa ctaatttgtt ttctttaacc aacttcccag ccagggctgc
     2401 catttcaccg ggtacgggat tctcttctac ccttatatgc gcgggcagct tcttgtgata
     2461 atcgagcgat ttcctgcggc cgaatttatt taccgctctt ctcagaaact ctgcggcctt
     2521 agtgtttttt atgagtttgc tcatcttgtg attgctcata atttttctaa ccgctgttct
     2581 cggatcagaa tctttactca ttgtcctgat tctgccgatg cttttccatc cgatagccag
     2641 ggaatacaac aaggtcgtgg ggcccgcgct gaaactgaag agatatcgat acccgagttc
     2701 gtacaaatcg ttcatgagaa acttcgtgag ttcgaggtaa acggcgttgt ttctgtgatc
     2761 aggatggata accgtgtctc ccgggcaaag ggccgtaaat ccgtctccgt tattacctaa
     2821 ttgccatttg ctttcattca gacaccgaac ggcgactact tttccttccc tgagggctag
     2881 atatattttc ggtaaagcgg cgtaaggatt gtcgaggtat ttccattgaa ggtaagattt
     2941 atttacagct gggtcaggtg tccaaatctc tttctcgagt tcacaaattt ggtcattgta
     3001 aaggggatcg taccttataa ttttgcagtt catcatgagc gaagaatatt ctccgatttt
     3061 ccaatagtgt ttttatcatt tggcttgcga atattttatt gccgacggtg ccatgtaaat
     3121 cgctgtcttc aaacgggcag gaaacggtta accgtctgtg tccggttatt tcatcgatat
     3181 agttcagggc tcggggccgc cgttacttat gttagttttg cattagcgcg gtgcgtgagc
     3241 ggcggaatcg ggcctcgttt aactgcggtg tta
//
LOCUS       DASSJH010000075         3443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_668353, whole genome shotgun sequence.
ACCESSION   DASSJH010000075 DASSJH010000000
VERSION     DASSJH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668353"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VFJ67_01620"
     CDS             complement(<1..1179)
                     /locus_tag="VFJ67_01620"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7289107.1"
                     /translation="MKKLTLMILMLTLLLPFGSLPSYAQSTEQEIQELKQMIQQNSKQ
                     NEELMRRIEQLESEKAATQVKMEKFMMEQEDKDLKYDGLMTFFDSIDLGFFVDTTYQY
                     VFNRGLTDSLQLRALYPDNQQFALNAFTISVSKTPSMEGGLMNLLGFRADILFGEQAP
                     ELGGEGFDSDVVDPYQAYLQVLAPVGNGINIFAGRFVTLAGYEVIEAWKDPNITRSIL
                     FGFAIPFTHTGIRTNYTAGPITFTAGLNNGWDQVEELNDSPTIESQLAYSYAGTTVSS
                     AWLGVTGYFGKEPSGLGFGGEGWRELITAVGTITFVEKITLIVDADFGWQQDVVFDDL
                     GAEDNVSWWGIAGYIVVDPHPAVRLSLRGEYFDDPEGYRTGLDQKLFEFTPTLILKPF
                     K"
     gene            complement(1662..3002)
                     /locus_tag="VFJ67_01625"
     CDS             complement(1662..3002)
                     /locus_tag="VFJ67_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7289108.1"
                     /translation="MNGGTSGNVNSRHGLFAWSKEINADQWRAFYAAFMGWTLDAMDL
                     LLFAFAVGSIGKVFNLSETWVGALFSVTLFSSAFGGALFGIVSDYIGRVKALTYTIIV
                     YSAFTGLSAFSPNIVFLLACRLILGLGMGGEWASGEVLVAEKWPKEHRGKVIGMVQSG
                     WGIGYILAAILATLIIPYFKAGQSYTIPLLNITLDGLDLGWRLLFFVGVAPAFLVFYI
                     RRHLDEPEVWKKTSDLRKEGKLSEKGQGFTLGQIFGADLIRYTITASLLTSLCMIAYW
                     GLYFWLPQFLASDKGVGLNTKGFIWIIPINIGAFIGCNTFGFISDKFGRRPVFVGYLL
                     VMAVLVWFFGHATSLRDVLILGPFIGFFGTGFYSGFGAIFSEIFPTRARGTAQGFCYN
                     VGRGVSAVAPPLIGFLVDKYGFAVGLTAVSVFAVAAAIVVLTLPETKGKALDIE"
     gene            3184..>3443
                     /locus_tag="VFJ67_01630"
     CDS             3184..>3443
                     /locus_tag="VFJ67_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET7289109.1"
                     /translation="MPNKYLYIETYGCQMNEYDSDRIRNALDAETTDNPAKADIIIIN
                     TCAIRDKADQKAFSSLGKYKHLKSKNPDLIVGISGCVAQLYG"
BASE COUNT          886 a          945 c          808 g          804 t
ORIGIN      
        1 cttgaagggt tttaaaatca gggtcggcgt aaattcgaag agcttctggt caagacctgt
       61 cctgtagcct tcggggtcat cgaagtattc acccctgagc gagagcctta cggccgggtg
      121 cgggtcgaca actatatatc ccgctatacc ccaccaggag acattgtcct cagcgccgag
      181 atcatcaaag accacgtcct gctgccatcc gaagtcagca tcgactatga gggttatctt
      241 ttccacgaat gtgatcgttc cgacagcggt tataagctcg cgccatccct ctcccccgaa
      301 cccgagcccg ctcggctcct tgccgaagta acccgtgact ccgagccagg cgctcgagac
      361 ggtcgtcccc gcgtagctgt atgcaagctg gctttctatt gtcggagaat cgttgagttc
      421 ctcgacctgg tcccatccgt tgttaagacc ggccgtgaaa gttataggcc ctgccgtgta
      481 attggtcctg ataccagtat gggtgaacgg aattgcaaat ccgaataata tcgatcgggt
      541 aatgttcggg tctttccagg cctcgattac ctcgtaaccg gcaagtgtca cgaaacgtcc
      601 ggcgaatata ttgatgccgt tcccaacagg agcaagcact tgcagatagg cctgataggg
      661 atctaccacg tcgctgtcga acccttcacc gcccaattca ggagcctgct ctccgaataa
      721 tatgtccgct ctgaaaccga gcaagttcat aagcccgcct tccatcgacg gcgtcttcga
      781 caccgagatc gtaaaagcgt tcagcgcgaa ctgctggttg tcgggataaa gagccctcag
      841 ctgaaggcta tccgtgaggc cgcggttaaa tacgtactga tacgtagtat cgacgaagaa
      901 cccgagatct atggagtcaa agaaagtcat aagaccatcg tatttcaagt ccttgtcttc
      961 ctgctccatc atgaactttt ccatcttcac ctgagtagcg gccttctcag attcgagctg
     1021 ttctatcctc ctcatcagct cttcgttctg cttgctgttc tgctgtatca tctgcttgag
     1081 ctcctggatc tcctgctccg tgctctgtgc gtatgaagga agcgaaccga acggcaacaa
     1141 aagagttaac atcaaaatca taagagttaa ctttttcatt tctgacctat cctcctttaa
     1201 attagtttat aagtcttatt tcttaatgag ccgacactag attcccggca gcacagacgg
     1261 ccaaaaaccc tccccgtgtc agtcgcaaaa tcagtctttc gattgttaat gagcaaaaga
     1321 tatgccacgt cgaaatccat taaggtccga acgggtgaat ttatcatttc cgcccattat
     1381 ctttgaatta tttggtagcc cgagagcgcc cggataggta tagataaatc aatctaaatg
     1441 cctagttatt aagcacttaa gtttgatttt aaggcatgca ccacactcgc tgaatttaat
     1501 cctgaggcat agaatcagaa ggacccggga cttttgaaat gtaggggatg attagcgtca
     1561 cattgcgtaa gacgagcaaa atattagaag tagcttgtat tttacaagga catgccgagg
     1621 acgctcgacg gaaaccgggt ttaattaccg ttaggtcaac actactctat gtccagcgcc
     1681 ttgcccttgg tttcggggag ggtaaggacc actattgcag ccgcgacagc gaatacagaa
     1741 acagccgtca gcccgacggc aaaaccatat ttatctacaa ggaacccgat tagcggagga
     1801 gccacagccg acactcccct gccgacgttg tagcaaaacc cctgggcggt ccccctggcc
     1861 ctcgtaggga atatttccga gaatattgcc ccgaagccgg aatagaaccc ggtgccgaaa
     1921 aacccgataa acgggcccag tatcagcacg tctctcaggc tggtcgcgtg gccgaaaaac
     1981 cataccagaa ccgccatcac caggagatag ccgacgaata cgggcctcct gccgaatttg
     2041 tcgcttataa atccgaatgt gttacacccg ataaacgcgc ctatattaat aggtatgatc
     2101 catataaaac ccttcgtgtt cagacccacg cccttgtcgc ttgccaggaa ctggggcagc
     2161 cagaaataga ggccccaata ggcgatcatg cacaggctcg taaggagcga agccgtaatc
     2221 gtatacctga tcagatcggc cccgaatatt tgtccgagcg tgaagccttg ccctttttcg
     2281 cttagtttcc cttctttcct gagatcgctt gttttcttcc agacttcggg ttcgtcgagg
     2341 tgcctcctga tataaaagac cagaaaggcg ggggcgactc ccacgaagaa gagcagcctc
     2401 cacccgaggt cgagcccgtc gagcgtgata ttgagcagcg gtatcgtata ggattgaccg
     2461 gccttaaagt acgggataat cagcgtggcc agtatcgccg cgagtatata acctattccc
     2521 cagccgctct gaaccatgcc gatgactttt cccctgtgtt ctttgggcca cttttccgcg
     2581 acgagcacct cgcccgaggc ccactcaccc cccatgccga gtccgaggat gagacggcag
     2641 gcgagaagga acactatgtt gggagaaaaa gcgctcaacc cggtgaacgc ggaatatact
     2701 attattgtat aagtgagcgc tttcacgcgg ccgatataat cggaaactat gccgaagagc
     2761 gcgccgccga aagcagagga aaaaagagtg accgaaaaca acgcgcccac ccatgtttcc
     2821 gagaggttga ataccttccc tatggacccg actgcgaagg caaacagcag caggtccatc
     2881 gcgtcgagcg tccagcccat aaatgccgcg taaaacgccc tccactggtc ggcattgatc
     2941 tcctttgacc aggcaaataa accgtgccgc gagttaacat ttccggatgt gccgccgttc
     3001 atctgtctct ctctcctgtc cccggcaact tttctccatc ctactaaatt tcccccacta
     3061 tgtcaatcgg ataagaaatc gtcagggcag ccctctaaat ataaaattta ctgtatctta
     3121 ggcgtttttt cacctccgtt atttcttgac tatgtgaagc gcgaacgtta catttttaat
     3181 tgaatgccca ataagtatct atacatagag acctacggct gtcagatgaa cgaatacgat
     3241 tccgacagaa tccgcaacgc actcgacgcc gagaccacgg acaacccggc aaaagccgat
     3301 ataatcataa tcaatacgtg cgccatacgg gataaagcgg atcagaaagc gttcagcagt
     3361 ctggggaaat acaaacacct taagtcgaaa aaccccgatc tcatcgtagg catctcgggc
     3421 tgcgttgcgc agctctacgg cga
//
LOCUS       DASSJH010000076         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_668484, whole genome shotgun sequence.
ACCESSION   DASSJH010000076 DASSJH010000000
VERSION     DASSJH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668484"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2006
                     /locus_tag="VFJ67_01635"
     CDS             <1..>2006
                     /locus_tag="VFJ67_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7289110.1"
                     /translation="ANVNLPTGALYGPDKSYTILATGQLYNAEAYKDIIVAYRDGSPV
                     RLRELADVIDSVEDDKNAAWFVTKDFQERAVILAIQRQPGTNTVAVADAVKEVIPSLQ
                     SYLPPSVSLNPLYDRSRSIRESMAEVNFTMGLTLGLVVMVIFLFLRNISATVIPSLAL
                     PLSIVGTFVAMYLCGFSLNNISMMALILATGFVVDDAIVMLENIVRHLEMGKKPLQAA
                     LDGSKEISFTLVSMTLSLAAVFIPVLLMGGVIGRIFKEFAVSITAAILISGFVSLTLT
                     PMLCSRFLRSEKEQKHGRFYNVTEKYFDSMFRAYEWSLGRVLEHRKAFLMLNGLILII
                     TALLFVKIPKGFLSNEDQGTIFTITEAPEGTSFEKMVEYQQQVADVLRESPYVRAFFS
                     SVGGGGAGTTGTNQGRMFMHLTPREERPTSFEIIDELRPRLAEIPGVSAFMQDQPEIR
                     IGGQLTKSLYQFTVQSPDIEELYKYVPLLEARMSEMPELKDVTSNLEIKNPQVNVEIM
                     RDKASAHGVSAAEIENTLYNAYGDRWVSTIYAPTNQFQVIMELKPEYQEDMNALSRLY
                     VKSSNDTLIPLETVARFTENVGPKTINHYGQFPAVTISFNLASGVSLSEAVDKIDKLV
                     TETLPGNMSTNYQGVAQEFQRSLSGLGLLLVFSVLVIYLILGIL"
BASE COUNT          461 a          541 c          566 g          438 t
ORIGIN      
        1 gctaacgtga acctgccgac gggagccctc tacggtcccg ataaatccta tacgatactg
       61 gcgaccgggc agctttataa tgcggaggcc tataaggata tcattgtcgc ttaccgcgac
      121 ggttcgcctg tccggctgag ggagctcgct gacgttatag acagtgttga ggacgacaag
      181 aacgcggctt ggttcgtgac caaagacttt caggaacgtg cggtaattct cgcgatccag
      241 cgccagccgg gtacgaatac ggtcgccgta gcggacgcgg tgaaggaagt cataccgtcg
      301 cttcaatctt acctgcctcc gagcgtaagc ctgaacccgc tttacgaccg ctcccggtcg
      361 atcagggagt ccatggcgga agtgaacttt acaatggggc ttactctcgg actcgtcgtc
      421 atggtcattt tcctgttcct gcgaaacata tccgcaacgg tcatccccag cctcgccctg
      481 ccactgtcga tcgtggggac cttcgtcgcc atgtatctct gcgggttcag cctcaacaac
      541 atctccatga tggcgctcat cctggcgacc gggttcgtgg tggacgacgc gatcgtaatg
      601 ctggaaaaca tagtaaggca cctggagatg gggaaaaagc cgctccaggc ggcgctcgac
      661 ggctcgaagg aaataagctt tacgctcgta tccatgactc tctctctggc cgcggttttc
      721 atcccggtgc ttctgatggg cggggttatc ggcaggatct tcaaggagtt cgccgtatcg
      781 attacggcag cgatacttat ttcaggtttc gtctcgctta cactaacgcc gatgctctgc
      841 agccgcttcc tccggagcga aaaggagcag aagcacgggc gcttctataa tgtgacggag
      901 aaatattttg actccatgtt cagggcatac gaatggagcc tcgggcgcgt gctcgagcac
      961 agaaaggcgt tcctcatgtt gaacgggtta attcttatta tcaccgcact gctttttgta
     1021 aagataccca aggggtttct gtctaacgag gaccagggaa cgatattcac gattacggag
     1081 gcgcccgagg gtacatcctt tgaaaagatg gtcgagtatc agcagcaggt cgccgatgtc
     1141 ctcagggaaa gcccttatgt cagggccttc ttctcaagcg tgggcggcgg cggggccggc
     1201 acaacgggga ccaaccaggg acgcatgttc atgcatctca ccccccggga ggaaaggccg
     1261 acctcgtttg agattataga cgagcttcgc cccaggctcg ccgagatacc gggggttagc
     1321 gcatttatgc aggaccagcc ggagataagg atcggcgggc agcttacgaa gagcctgtat
     1381 cagttcaccg tacagagccc cgatattgaa gagctgtata agtatgtccc tctgctcgag
     1441 gcccggatga gcgagatgcc cgaattaaag gacgtgacca gcaacctcga aatcaagaac
     1501 ccccaggtga acgtggagat aatgagggac aaggcctccg cccacggggt atcggccgcc
     1561 gagatcgaaa acacgcttta taatgcctac ggagacaggt gggtgtcgac tatttacgcg
     1621 cccactaacc agttccaggt cattatggag ctcaagccgg agtatcagga agatatgaac
     1681 gccctttcga ggctgtacgt caagtcatcg aacgacacgc tcataccgct cgagacggtc
     1741 gcgagattca cagagaacgt cggccccaag actataaatc actacgggca gttcccggcg
     1801 gtgaccatat ctttcaacct ggcctcaggc gtgtctttga gcgaagcggt cgataagata
     1861 gataaactgg tcacggaaac gctgcccggc aacatgagca cgaattatca gggcgtagca
     1921 caggagttcc agagatcgct cagcggtctg gggcttcttc ttgtcttttc ggttctggtc
     1981 atttacctga ttctaggcat tctcta
//
LOCUS       DASSJH010000077         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_126984, whole genome shotgun sequence.
ACCESSION   DASSJH010000077 DASSJH010000000
VERSION     DASSJH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126984"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1387
                     /locus_tag="VFJ67_01640"
     CDS             170..1387
                     /locus_tag="VFJ67_01640"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HET7289111.1"
                     /translation="MGLNEQKEKPGAILVINSGSSSLKFSVFTADDSLRLFLRGQIES
                     LFTAPHFIAHDGDGNIKDEKTWPRGTKLGHDGAIKHLFDFGGSGVMGGYKICAAGHRV
                     VHGGTLFTVPVLVNESVIESLEKLVPLAPLHQPYNIEAIRAVGKRAPELHQVACFDTS
                     FHRTQSDLERTYAIPRRYTEEGVRRYGFHGLSYEYISSALAEYDSTLASGRVVIAHLG
                     NGASMCAVSAGKSVATTMGFTAVCGLPMGTRTGTIDPGILIYMMDNYGMDARAVEDLI
                     YNRSGLLGVSGVSSDMRTLLESGDPRAKEAVNLFIYRIGRELGSLAAALGGLDGIVFT
                     AGIGENAAYVREKVCLDAEWLGVVLDKEANDAGGPGISKKGSRASAWVIPTNEELVIA
                     AHTERLLERSSKK"
     gene            complement(1505..1777)
                     /locus_tag="VFJ67_01645"
     CDS             complement(1505..1777)
                     /locus_tag="VFJ67_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET7289112.1"
                     /translation="MSSDNNLCEHFNGWQPIPPKTHVCEKCVEMGDKWFHLRACQTCG
                     AVLCCDSSKNKHASKHYRETGHPIVISAERGERWAWCYSDELYVEY"
     gene            complement(1805..>2598)
                     /locus_tag="VFJ67_01650"
     CDS             complement(1805..>2598)
                     /locus_tag="VFJ67_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7289113.1"
                     /translation="ITQATRLGAEVLTPQEVTDIKLKDMYKIIKLSDGKELNALSLII
                     ASGVDYRRLDAEYVNDFTGAGIYYGSCRTEAHACKGKDVYVVGGGNSAGQAAMYLSKF
                     AKNVFIVIRGESLGFSMSQYLIEQISRTPNISILSRSRIVEVRGDGRLESIVIEDVDT
                     GERRTAETQSLFIFIGMRPFTDWIKLDLIKNEKGYIETGRDLFNYESFKSIWKLERSP
                     FLLESSIPGIFAAGDVRSGAMNRVASAVGEGSMAIKFIHEYINRS"
BASE COUNT          621 a          731 c          689 g          557 t
ORIGIN      
        1 atccgcatcc tccatagcga tcaattcgat ctgcctctga acgaaaaaca gcttaaaacg
       61 gtcaaagaga tcgagaccag attgaaagag ctgggctgcc ccaaagaccg ctggaagaag
      121 cgccccgcct gaatggcgta agagttccta atccggagat agaaccgaca tgggtctgaa
      181 tgagcaaaaa gagaagccgg gagctatact cgtcataaac tcgggctctt cgagcctgaa
      241 attctccgta ttcaccgctg atgattctct gcggcttttc ctccgcggtc agatagaaag
      301 tttattcacg gctccccatt tcatagcgca cgacggggac ggaaacataa aggatgaaaa
      361 aacttggccc cgcgggacga aactcggtca cgatggggcg atcaagcact tattcgattt
      421 cggcggttcg ggagtgatgg gaggatataa aatctgcgcg gccggccaca gggtggtcca
      481 cggcgggaca ctgtttacag tgcccgtgct cgtgaacgaa agtgtgatcg agtccctgga
      541 aaaactcgtg ccgctcgccc cactccacca gccatacaac atagaggcca tcagagccgt
      601 gggcaaaagg gccccggagc tacaccaggt cgcctgtttc gacacctctt ttcaccgcac
      661 tcaatccgac ctcgaacgca cttacgccat accgaggaga tatacggaag aaggggtcag
      721 gcgttacggc ttccacggcc tctcgtacga atatataagc tcggcattgg cggaatacga
      781 cagcacgctc gcctcggggc gggtcgtcat tgcacacctc ggcaacgggg caagcatgtg
      841 cgctgtaagt gcaggaaaga gcgtcgccac caccatgggg ttcaccgccg tttgcggtct
      901 gcccatggga acgcgcacag ggacaataga ccccggcata ctcatttaca tgatggacaa
      961 ttacgggatg gacgcacgag ccgtggaaga cctcatctat aaccgatcgg gactgctcgg
     1021 cgtatcgggc gtatcgagcg acatgcgcac gctcctcgaa agcggcgacc ctcgcgcgaa
     1081 agaggcggtc aaccttttta tatacaggat aggacgggag ctgggctcgc tagccgcggc
     1141 gctcggaggc ctcgacggaa tagttttcac ggccggcata ggcgagaacg ccgcgtacgt
     1201 aagggaaaaa gtgtgccttg acgccgaatg gctcggcgtc gtgctcgaca aggaagcgaa
     1261 tgatgcagga gggcccggga ttagcaagaa ggggagccgg gcttcagcct gggtcatacc
     1321 gactaacgag gagctagtga tcgccgctca taccgaacga ctattggaac gatcgagtaa
     1381 aaagtagaat ggaaaacaac gcccggcgaa caagatatcg aaggatgtat tcaatgcccc
     1441 gtcagcatct tccccagcag ccttccggac ccggctccgt tgcccgcaaa cactatttca
     1501 ttcatcagta ttcgacgtaa agctcatccg aataacacca cgcccacctc tccccgcgct
     1561 cggccgaaat aactatcggg tggcccgtct cccggtagtg cttcgaagcg tgcttgttct
     1621 tggaggagtc gcagcagagt acagccccgc aagtctgaca ggccctcagg tggaaccact
     1681 tatcccccat ctcgacacat ttctcgcaga catgtgtctt gggaggaatg ggctgccacc
     1741 cgttgaaatg ttcacagagg ttgttatccg atgacattcg gcgctccggc aatgaagtga
     1801 tggttcacga tctgtttatg tactcgtgta tgaatttgat ggccatggac ccttcaccga
     1861 ccgccgaagc gacccggttc atagcacccg acctgacgtc tcctgccgcg aatattccgg
     1921 ggatgctgga ttcaagaaga aaggggcttc gctcgagctt ccaaatcgac ttgaagctct
     1981 cgtaattaaa gaggtccctg ccagtctcga tatacccctt ctcgttcttt atcagatcga
     2041 gctttatcca gtccgtgaaa ggacgcatac ctatgaatat gaacaatgat tgagtctcgg
     2101 ccgttcttct ctctcccgta tccacgtcct cgataactat gctttcgagc ctgccgtccc
     2161 ctcgcacctc gactatacgg ctcctgctta aaattgaaat attgggcgtg cgggatatct
     2221 gctcgatcag atactgcgac atcgaaaaac cgagagattc tccccttatg acaatgaata
     2281 cgttttttgc gaatttcgat aaatacatgg cggcctgccc cgcggagttc ccaccgccca
     2341 ctacgtagac gtctttccct ttgcaggcgt gggcttccgt tctgcaggag ccgtaatata
     2401 tgccggcgcc ggtaaaatcg ttcacgtact ccgcatccaa acgtctgtaa tcgacgcccg
     2461 aggcgattat cagactgagc gcgtttaact cctttccgtc cgagagcttg atgatcttgt
     2521 acatgtcttt gagctttata tcggttactt cctgaggagt aagcacttcg gcgccgagcc
     2581 tcgtcgcctg agtgatcg
//
LOCUS       DASSJH010000078         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1208097, whole genome shotgun sequence.
ACCESSION   DASSJH010000078 DASSJH010000000
VERSION     DASSJH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208097"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /gene="gatB"
                     /locus_tag="VFJ67_01655"
     CDS             <1..981
                     /gene="gatB"
                     /locus_tag="VFJ67_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET7289114.1"
                     /translation="LVEIVSEPDISNPEEAVQYLKKLRSILMYIGVCDGNMEEGSLRC
                     DANVSVRPQGSKALGTKVEIKNVNSFKFIQKAIDYEIRRQVSVIEGGGTIVQETRLFD
                     AQKGITFSMRSKEEAHDYRYFPDPDLLPVVIAEEKIEELRASLPELPDQRQMRFISEY
                     KLPEYDASVLTASKQIADYFEECLEHYDNPKAVSNWIMTELMREMKEEGDAGEIPVGP
                     GNLAQLLQLIDDGTISGKIAKDVFQEMVSTGKTAKEIVGEKGIKQISDRGELETIIAD
                     ILKNHPGEISRYKGGDEKLIGFFVGKVMKATQGKANPKVVNEILMAELSK"
     gene            complement(1029..2117)
                     /locus_tag="VFJ67_01660"
     CDS             complement(1029..2117)
                     /locus_tag="VFJ67_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7289115.1"
                     /translation="MKTGEKILIVDDEAAIRSLFVEALEELGYRCDVAGNGLECLEKF
                     YRVKDYDVVLLDIQMPKLNGLETLKKLKAYSPDLSIIMVSASRDIENVRAALKEGAYD
                     YVFKPFNVNDVDAVIRRALERSNLIKANKDYQKNLEKKVMEQTQELVRVYSGTLEAMI
                     LALDLREHETGYHSYRVTEYAINLGKHMRLSDEELSIIAKGALLHDIGKIGVPDHILL
                     KPDKLTEEEWELMRKHAEFGYELLKKIDFLEHSAKIVHTHHERYDGQGYPSGLSGEDI
                     PLGSRIFSVVDALDAMTSRRTYRKATSFEDAVQRIAEASGTQFDPKVIEVFMQVPVEE
                     WKSIKKRVASSSTEYLQQLMFELSKYRV"
     gene            2346..>2601
                     /locus_tag="VFJ67_01665"
     CDS             2346..>2601
                     /locus_tag="VFJ67_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HET7289116.1"
                     /translation="MKKATLVIEDGSVFEGLSFGAVGEAYGEVVFNTSITGYQEILTD
                     PSYNGQIVAMTYPEIGNYGVNPEDVESRKPFLRGFVVKEYW"
BASE COUNT          658 a          659 c          665 g          619 t
ORIGIN      
        1 ctcgtcgaaa tagtgagcga gcccgacatc tcgaaccctg aggaggcagt tcagtatctg
       61 aaaaaactgc gctcgatact tatgtatata ggcgtttgcg acggaaacat ggaggaagga
      121 agcctgaggt gtgacgccaa cgtgtctgtg cgtccccagg gcagcaaggc cctcggcacc
      181 aaggtagaga taaagaacgt gaactcgttc aagtttatac agaaagcgat tgattacgag
      241 ataaggagac aggtgtccgt catcgaaggc ggtggtacca tcgtgcagga gacgaggttg
      301 ttcgacgctc agaagggcat tacgttctcg atgaggtcga aagaggaagc gcacgattac
      361 aggtatttcc cggacccgga cctcctgccc gtagtgatag ccgaagagaa gatagaggag
      421 ctgcgcgcgt cgctccccga actgcccgat caaaggcaaa tgaggttcat aagcgaatac
      481 aagctccccg agtatgacgc gtccgtgctt accgcgtcaa agcagatcgc cgactatttc
      541 gaggaatgcc tcgagcatta cgacaacccg aaagccgtaa gcaactggat aatgactgag
      601 ctcatgcggg agatgaagga ggagggagac gccggggaaa tccccgtcgg cccaggaaac
      661 ctcgctcagc ttctccaatt gatagacgac gggacgataa gcgggaagat cgcgaaggac
      721 gtttttcagg aaatggtctc gacgggcaag accgcgaagg agatcgtcgg ggagaaagga
      781 ataaagcaga tatctgacag gggcgagctc gaaacgataa tagcagatat attgaagaat
      841 caccccggag agatatctag atataagggc ggagacgaga agctcatagg cttcttcgtc
      901 ggaaaggtga tgaaagctac tcaggggaag gcaaacccga aggtcgtgaa cgagatactg
      961 atggcagagc tttcgaaata gcactataaa taaaatccgg gaattgttcg tggaattgta
     1021 accgacgatc agactctgta tttgctgagc tcgaacatga gctgctggag gtattcggtg
     1081 ctggagctcg cgacgcgttt cttgatgctc ttccattcct cgaccggaac ctgcatgaac
     1141 acctcgatca ctttcggatc gaactgagtg cccgaagcct ccgctatcct ctgcaccgcg
     1201 tcctcgaaag atgtagcttt cctgtaggtc ctgcgggagg tcatggcgtc gagcgcgtcc
     1261 acgacggaga atatcctcga gcccagcggg atgtcttctc ccgaaagtcc cgacggataa
     1321 ccctgcccgt cgtaccgctc gtggtgtgta tggacaatct tcgccgaatg ctcgagaaaa
     1381 tctatcttct ttaaaagctc ataaccgaac tcagcgtgct tcctcatcag ctcccattct
     1441 tcctccgtga gcttgtcggg cttgagaaga atatggtcgg gcacgcctat ctttccgata
     1501 tcgtgaagca gcgccccttt cgctattatc gaaagctctt catcggagag cctcatatgc
     1561 tttccgagat ttatcgcata ctccgtgacc ctgtaggagt gataccctgt ctcgtgctcc
     1621 ctcagatcga gcgccagtat catcgcctcg agagtgccgg aataaaccct cacgagctcc
     1681 tgcgtctgct ccatcacctt cttttcgagg ttcttctggt aatccttatt cgcttttata
     1741 agattcgatc tctcgagcgc acgccttatc acggcatcca catcgtttac gttgaaaggc
     1801 ttaaagacgt aatcatacgc cccctctttc agggcggccc tgacgttttc tatgtcgcgc
     1861 gacgctgata ccataattat ggaaagatcg ggcgaatagg cctttaactt tttgagcgtc
     1921 tcaaggccgt tcagcttcgg catctgtata tcgagaagga cgacgtcata gtcttttacc
     1981 cggtagaact tctcgagaca ctcgagaccg ttccccgcga cgtcgcacct gtacccgagc
     2041 tcttcgagag cctcgacgaa caggctccta atagcagcct cgtcgtccac tataaggatt
     2101 ttctctcccg ttttcataac catatcctcg gataatctat ttgccgtttc cataatcagt
     2161 ttattgaaat cggaataacc cgcctctgtc cgttaattcg ctgaacgcgg tcggtgatcc
     2221 agtttgtgtt tgatgtaatc cgttacccat caccccgatg aataacgtat tttcccctta
     2281 tagtataact atactgtcaa aagtttgaca cgtgcatttt ttgtatgtat tttccaaccg
     2341 aaatcatgaa aaaagcgact ctcgtgattg aagacggaag tgttttcgag ggtttaagct
     2401 tcggggcggt gggcgaggct tacggagagg tcgttttcaa tacatccatt acgggctacc
     2461 aggaaatact gaccgacccc tcttacaacg gccagatcgt agccatgact tatcctgaga
     2521 taggcaatta cggcgttaac cccgaagacg tcgaatcgag aaaacctttc ttaaggggct
     2581 tcgtcgttaa agaatattgg a
//
LOCUS       DASSJH010000079         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_777474, whole genome shotgun sequence.
ACCESSION   DASSJH010000079 DASSJH010000000
VERSION     DASSJH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777474"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VFJ67_01670"
     CDS             complement(<1..747)
                     /locus_tag="VFJ67_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HET7289117.1"
                     /translation="MKQVTVFLFCISLFLAAPAALAEDGPDKGQSSFLSSLSTSEHMT
                     GNWGGLRDRLIEWGLTPTAEYGATLQGNPVGGMRKGIKYAGLLDVYLDFDLEKLLRLG
                     GTRLVVSGAWAYGENLSAHDIGNFFTVSNDFNGKFVGLYQLFIETNLWKDRLTIAVGR
                     MGIGDDFGTADVFGIYVSSVVNLNPISLAYNIPAYLSNPDAALGARLRVKLVRDFYIA
                     AGVYNADPEAAVNQRVSIDFDFTFNDGVILI"
     gene            complement(873..2582)
                     /locus_tag="VFJ67_01675"
     CDS             complement(873..2582)
                     /locus_tag="VFJ67_01675"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HET7289118.1"
                     /translation="MNKLCMVSLSAVLIALISFGCGGKKPGTASKTTPEFTPPIYQDT
                     SDFVKPPCSTPDVRITQGSLCGSVISTAAGKEVNAYLGIPFAESTGGQNRWKPPVPRT
                     PWQGTFRATRLGPACPQNTDNMYPESEDCLSVNVWTPADAHTEPRAVMVFIYGGAFIY
                     GYNADPLYDGAYTAARGDVVVVSMNYRLGALGFLSGITDKKTGEEINGNFGILDQILA
                     LKWVRDNITAFGGDLDKVTIYGESAGAMSVGLHLLSSPGSEPLFRAGIMESNPLGLPY
                     KSLKQSHSIAKEFASNLGCPADDVGCMRAALPEEVLDAQEQKDFIWPALFHGVKDMLV
                     WAPVIDGRVLTRQPVEAAADGKLTKPVIVGTNTNEALLFVERAKLGLGWKTVSAFDYR
                     LTMDFIFRDRGLREKIYGKYPPLNGDNTALISKVLTEYLFTCPSLDAASHASQETWSY
                     IFDHVPSFNVWPMVPACADAVCHAAELSYVFHTPEGRKGRFTPRENDLSNLMIGYWTG
                     FAKTGRPDGGGSAWPEFDPGSLNLVFVTPVDDITAKPDTASDCGFWDGIGYNLHNSFW
                     GMF"
     gene            complement(2608..3105)
                     /locus_tag="VFJ67_01680"
     CDS             complement(2608..3105)
                     /locus_tag="VFJ67_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7289119.1"
                     /translation="MPIYEFYCKKCNTVYKFYSRTIDTSTVPKCPGCKRVRLERIFSS
                     FATLSKRESEESADAGMPDVDESKLERAMAMLEKETRGMDENDPTQAADLMRKLTDAA
                     GISLGEGMEEALSRMEKGEDPDKIEEELGDLLGEESFSFESKKKKRNTKSRARPNVDE
                     KLYEL"
BASE COUNT          715 a          990 c          948 g          809 t
ORIGIN      
        1 tatcagtatc acgccgtcat tgaaagtgaa atcgaagtct atcgacaccc tctgattgac
       61 ggccgcctcg ggatcggcgt tatacacacc ggcggcgata taaaaatcgc ggaccagctt
      121 tacgcgcagg cgcgccccca gagcggcgtc cgggttgctg aggtaggccg ggatattgta
      181 ggccaggctt atagggttca aatttacaac ggagctgacg taaattccga acacgtcggc
      241 cgtgccgaaa tcgtccccta tccccatgcg tccgaccgca atagtgagcc tgtctttcca
      301 caaattcgtt tcgatgaaaa gctggtaaag acccacgaac ttgccgttga aatcattcga
      361 cactgtgaag aaattcccta tatcatgggc gctgagattc tcaccgtatg cccacgcgcc
      421 cgatacaact aacctcgtcc cgccgagcct gagcagtttt tcgaggtcga aatcgagata
      481 gacgtccagg agccccgcgt actttatgcc ctttctcatc cctcccaccg gattgccctg
      541 cagggtggcg ccgtactcag ccgtgggtgt gagcccccat tcgatcagcc tgtccctgag
      601 accgccccaa ttgcccgtca tgtgctcgct cgtggaaaga gacgacagga aagagctctg
      661 tcccttgtcc gggccgtcct cagcgagcgc ggccggtgcc gccaggaata aagagataca
      721 gaacaagaaa accgtgacct gtttcataaa ggggcggttt gagccgcctt acgaccttta
      781 acgagagcga tagcaaattc taccgcccgg cagatgtacc tgtaaacaac cgttttcaaa
      841 acaggaagag aatgttcaat cacgatgtta gcctagaaca tcccccagaa cgagttatgg
      901 aggttatatc ctatgccgtc ccagaacccg caatccgagg ccgtgtcggg ctttgccgta
      961 atatcgtcaa ccggggttac gaatacgagg tttagcgagc cgggatcgaa ttcgggccag
     1021 gcagatcctc caccgtccgg gcggccggtc ttcgcgaagc cggtccagta tcctatcata
     1081 aggttggaga ggtcgttttc ccggggagta aatctacctt tcctgccttc gggtgtgtgg
     1141 aatacgtacg agagctcggc tgcgtgacac acggcgtcgg cgcaggcggg aaccatcggc
     1201 cagacgttga acgagggaac gtgatcaaag atgtatgacc acgtctcttg agatgcatga
     1261 gaagctgcgt cgaggctcgg gcacgtaaag aggtattccg taaggacttt cgatatgagc
     1321 gcggtattgt ccccgttcag aggagggtat ttcccgtata ttttctcccg aagccctcta
     1381 tcccggaaga tgaaatccat cgtaagcctg taatcgaaag cggagaccgt tttccatccg
     1441 agccctaatt tcgccctctc cacgaataac agcgcttcgt tcgtattcgt gccgacgatc
     1501 acgggtttgg tcagcttgcc gtcggcggcc gcttcgacag gctggcgtgt caggacccgg
     1561 ccgtctatga cgggggccca gacgagcatg tccttcacgc cgtggaagag cgcgggccat
     1621 atgaaatctt tctgctcttg agcatcgaga acctcctcgg ggagcgccgc cctcatacac
     1681 ccgacgtcgt ccgccggaca gccgaggttg gaggcgaatt ccttcgctat cgagtgtgac
     1741 tgcttcaggc ttttgtaagg cagcccgagc gggttgctct ccataatgcc tgctctgaag
     1801 agaggctcgg acccgggaga tgaaagcaga tggagcccta cgctcatggc gcccgcgctc
     1861 tcgccgtata tagtcacctt gtccaggtcg ccgccgaagg cagttatgtt gtcgcgcacc
     1921 cattttagcg cgagtatctg gtcgagtatg ccgaagttgc catttatttc ctcgcccgtt
     1981 tttttgtccg ttatgccgga gagaaatccg agggcgccga gcctgtagtt catgctcacc
     2041 acgacgacgt ctccccgggc ggcggtgtaa gcgccgtcgt aaagggggtc ggcgttatat
     2101 ccgtatatga acgcgccgcc gtatataaag accatcacgg cccggggctc ggtatgcgcg
     2161 tccgcgggcg tccagacgtt tacgctgaga cagtcctcgc tctcggggta catattgtcc
     2221 gtattctggg gacaggcggg gcctagcctc gtagccctga acgtgccctg ccatggcgtc
     2281 ctgggcacgg gcggcttcca tcggttctga ccgccggtcg attccgcgaa aggaattccc
     2341 agatacgcgt ttacctcttt accggcggcc gtgcttatga cgctcccgca gagagaaccc
     2401 tgtgtaatcc gcacatccgg cgtagagcac gggggcttta cgaagtcaga agtgtcctga
     2461 tagatgggcg gcgtgaattc gggagttgtc ttcgaggctg tgcccggctt tttcccgccg
     2521 cagccgaagg agattaacgc gattagaact gcagagagag aaaccatgca gagtttattc
     2581 acgcttcttc tttttccgga gacccgatca gagctcgtaa agtttttcat ccacgttcgg
     2641 cctggccctg cttttcgtgt ttcttttttt ctttttggat tcgaagctga acgattcctc
     2701 tcccaacaaa tcgccgagct cctcctctat cttatcgggg tcctcgccct tttccatgcg
     2761 ggaaagagcc tcctccatgc cttctccgag cgagatgccg gctgcgtccg tgagcttcct
     2821 catgaggtcc gccgcctgag tggggtcgtt ctcgtccatg ccccgggttt ccttctcgag
     2881 cattgccatc gccctctcga gcttcgactc gtctacatcc ggcatccccg catcagcgct
     2941 ttcctcgctt tctcttttgc tcagcgttgc gaacgaggag aatatccttt cgagcctgac
     3001 ccttttgcat cccgggcact tcggtacggt ggacgtgtct atcgtgcggg agtagaactt
     3061 ataaactgta ttgcatttct tgcagtagaa ctcgtagatg ggcatattgg caccgcgcca
     3121 ggaatctatt atacacaaat tcgagtgtaa cttgccagca gaaacgtgat ccggaattga
     3181 gcggtttaga cgcagtctat gtcggggatt taaaagtgca cacttcgacg agctcagggc
     3241 ttcccggcta aggctcgggc ctcccagcta cggcttggcc tacgccgtga agagtcgccg
     3301 ggacgagcgg tgatctctct tctcctctcc gcggattgta tggctattta tggatatatt
     3361 acatatatag catatacaac ctgcctgatt tcaccgcgga gtgccaaatg agcggcgtgt
     3421 aaaagatatc cgtccaaaac ccggaatccg gtgaaccctt gg
//
LOCUS       DASSJH010000080         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1208376, whole genome shotgun sequence.
ACCESSION   DASSJH010000080 DASSJH010000000
VERSION     DASSJH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208376"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /gene="ptsP"
                     /locus_tag="VFJ67_01685"
     CDS             <1..1443
                     /gene="ptsP"
                     /locus_tag="VFJ67_01685"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET7289120.1"
                     /translation="PAELAVIEGIEPSAQQFEALSDKYLREKAQDFRDIGERILHELV
                     RHKKGRSIVTEPGEGSVLVAYDIGPSFISMLFGNKVSGVVIERGGETSHAVIIAKSLG
                     IPAVVGIDNICSLLKSGERAIVDGKTGFIFSNPDESLIAEYRNTYSKLMKVREVIEEA
                     GKENTDNNGLGVKISANIGFPIDTQLAKQYGIKDVGLFRTEFAFTQYPKWPGIKDQVR
                     IYNELAKDFEGYVTVRTLDIGADKLLPYFEFPKEDNPLLGLRAIRFSMEYLDLFRNQI
                     GAILLASARGHRFRILLPMITNLWEVETAKEIIEQMAGEAGLTSSKVPPLGIMLEVPA
                     ILYQLDDYKDMIDFISVGTNDLVQYILAVDRNSNVVGHLYSGFHPAVLRVLDDIYYKT
                     RYSGIDVSVCGELAGTPSGALCLIALGYRQLSISPSHVPYIRYLCKKLDKYTLGTVKR
                     DILALKKKADIERYLVETLESIDPALLEIE"
     gene            1512..2411
                     /locus_tag="VFJ67_01690"
     CDS             1512..2411
                     /locus_tag="VFJ67_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7289121.1"
                     /translation="MGKIHIILFILTVITTFVTGYSFSGSVASAFAFSGTLLFILGSH
                     EMGHYFFGRKYGVDITPPYFIPAPPIISPIGTFGAFIKIKSPISTRKALFDIGIAGPL
                     AGIVASIPVLLVGIKYSTIIDVADKEGGQGLMLGSPLIFTFFTSIFFGKIPEGKDLFL
                     HPVAFAGWVGLFVTALNLIPSGQLDGGHIVYSLFPKKAHRVTSVVMIAILLVFGFGTE
                     PLFMLYHRLAGSGPGDLLSRIPDFEGWPGWILWAVLLTLMGTKHPPTIHDEGELDTGR
                     KLLGFISLLIFIGCITPVPIKLT"
     gene            2644..>2833
                     /locus_tag="VFJ67_01695"
     CDS             2644..>2833
                     /locus_tag="VFJ67_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HET7289122.1"
                     /translation="MAKDTLSITDNRTGKSYEIPIENDTIRAADLKQIKVNDDDFGMM
                     SYDPAFGNTAACISKVTYI"
BASE COUNT          687 a          816 c          763 g          567 t
ORIGIN      
        1 ccggccgagc tcgcggtaat agagggcata gaacccagtg cccagcagtt cgaggctcta
       61 tccgataaat acctgaggga gaaagcccag gacttcaggg acatcggcga gaggattctc
      121 cacgagctcg tcaggcacaa gaaggggagg agcatagtga ccgagccggg cgaaggctcg
      181 gtgctcgtcg cctacgacat aggcccgtca ttcatatcca tgctgttcgg caacaaggtc
      241 tcgggcgtcg tcatcgaaag gggcggcgag acctcccacg cggtcatcat agcgaagtcg
      301 ctcggcatcc ccgcggtcgt cggcatagac aacatatgca gcctcctcaa atcaggggag
      361 agggcgatag tcgacggcaa gaccgggttc atattctcta accccgacga gagcctcata
      421 gccgagtaca ggaacaccta cagcaagctc atgaaggtga gggaggtcat tgaagaggcc
      481 ggcaaggaaa acaccgacaa caacggactc ggagtgaaga tatcggcgaa tatcggattc
      541 ccgatagaca cacagctcgc caagcaatac gggataaagg acgtgggcct cttccgcacg
      601 gaattcgcct tcacgcagta ccccaagtgg ccggggataa aggaccaggt gaggatatac
      661 aacgagctcg ccaaggactt cgagggctac gtcaccgtca ggaccctcga catcggggcc
      721 gacaagctcc tcccttattt cgagttcccc aaggaggaca accccctcct cgggctccgc
      781 gcgataaggt tctcgatgga gtacctcgac ctcttcagga accagatcgg ggcgatactc
      841 ctcgcctcgg ccaggggaca ccgcttcagg atactcctcc cgatgataac caacctctgg
      901 gaggtcgaga ccgcgaagga gataatagag cagatggcgg gcgaggcggg gcttacatcg
      961 tccaaggtac cgcccctcgg aataatgctc gaagtaccgg cgatcctcta ccagctcgac
     1021 gactacaagg acatgatcga cttcatttcg gtagggacga acgacctcgt ccagtacata
     1081 ctggccgtcg acaggaactc gaacgtcgtg gggcacctgt attcgggctt ccaccccgcc
     1141 gtgcttcggg tgctggacga catatactac aagacgagat attcgggcat cgacgtctcg
     1201 gtatgcggcg agctcgcagg caccccttca ggcgctctct gccttatcgc gctcgggtac
     1261 aggcagctca gcatctcgcc ctctcacgtc ccctacatca ggtacctgtg caagaagctc
     1321 gacaaataca cgctcggcac ggtcaagcgc gacatactcg cgctcaagaa aaaggcggac
     1381 atcgagagat atttggtcga aaccctggag agcatagacc cggcgctcct cgagatcgaa
     1441 taaatgaagc ttcccttgcg cccccttcca atttccgatt actgaggtac tttttacacc
     1501 ctgccatgaa agtgggaaag atacacataa ttctgttcat acttaccgta atcacgacgt
     1561 tcgtcacggg atactcgttc agcggctcgg tcgcgagcgc gttcgcgttc tcgggcacgc
     1621 ttttgttcat actcggctct catgagatgg ggcattactt ctttgggcgg aaatacgggg
     1681 tcgacatcac gccgccttat ttcattcccg cccctcccat catttcaccc ataggcacat
     1741 tcggggcctt tatcaaaata aagtcgccca tatcgacccg caaagccctt ttcgacatag
     1801 gcatagcggg cccgctcgcg gggatcgtcg cgagcatccc ggtgctcctc gtaggcatca
     1861 agtactcgac gatcatcgac gtcgcggaca aagaaggagg gcagggactc atgctcggct
     1921 cgccgcttat attcactttt ttcacctcga tatttttcgg aaagataccg gaagggaaag
     1981 acctcttcct ccatcccgtg gccttcgccg gctgggtagg gctcttcgtc acggccctta
     2041 acctcatacc ttcgggccag ctcgacgggg ggcacatcgt gtattcgttg ttcccgaaga
     2101 aggcgcacag ggtcacgtcc gtagtcatga tcgccatcct gctggtcttc ggcttcggga
     2161 cagagcccct gttcatgctt taccacaggc tcgcgggcag cggtccgggc gatctcctga
     2221 gccgtatccc cgatttcgag ggctggccgg gctggatact ctgggccgtt ctcctgacgc
     2281 tcatggggac caagcacccg cccaccatac acgacgaggg ggagctcgat accgggagaa
     2341 aactcctcgg atttatttca cttttgatat tcatagggtg cattacgccc gtgcccataa
     2401 agctgacata gccggccgtt aagcacgccc tgggaacatg gtgaaacagg ccgggagctt
     2461 tctcgaaatg agccgtccgg aggccgattc aatatttttt ataaatattg ataatttcgg
     2521 gagttagggt aagtttacat gtccgaaacg ctcgtatttg ggacaaaaaa tccttaacaa
     2581 ttgatctggc tataaacata aaaaacctcg cagggcaaca aaagcgtata aggaggaacg
     2641 atcgtggcta aggacacgtt atccataaca gacaacagga cgggcaagtc ctacgaaata
     2701 cccatcgaaa acgacaccat aagggctgcc gacttaaagc agataaaggt gaacgacgac
     2761 gatttcggca tgatgagcta tgacccggcc ttcggaaaca ccgcagcctg tataagcaag
     2821 gttacctaca tcg
//
LOCUS       DASSJH010000081         7977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_778036, whole genome shotgun sequence.
ACCESSION   DASSJH010000081 DASSJH010000000
VERSION     DASSJH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778036"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VFJ67_01700"
     CDS             <1..1127
                     /locus_tag="VFJ67_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET7289123.1"
                     /translation="ELIKTSLVPDFYELTPEKFNNKTNGITQRRWLLDCNPRLSSLIN
                     RTVGDGWIRDLYKLRPLENYSQDEGFQGEFMRVKQINKENLARLIHTITRITVDPESL
                     YDVQIKRIHEYKRQLLNIMHIIHQYFRVVEDGIELNVPRTYIFAGKAAPGYWAAKQII
                     KLINNVADVVNSDPRVGGQIRVIFMPDYKVSLAEKIIPAADLSEQISTAGKEASGTGN
                     MKLALNGSLTIGTMDGANIEIREEVGGENIFIFGLRAHEIAEMKKTHSYHPREICEKN
                     PYLKRVMDSFESDVFCKEERGLFKWIYEALLINGDTYFHIADFESYAETQKTVGQEYK
                     NTGDWARKAILNVARIGKFSSDRTISEYAEDIWKVGPLKD"
     gene            1217..1768
                     /gene="pyrE"
                     /locus_tag="VFJ67_01705"
     CDS             1217..1768
                     /gene="pyrE"
                     /locus_tag="VFJ67_01705"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET7289124.1"
                     /translation="MASEKKERLKLILLGNSILRGKFTLASGKESDYYIDARLTTLDP
                     EGVSIVADIFLEEIRRDPLIKTVGGPTMGADPIVGALLGESHRGNYPLRGFLVRKQEK
                     EHGTGKMIEGNLRAGDSTAVVEDVVTSGGSVITAINAVKSAGAHVRKVLVIVDREEGA
                     AEKFREMGIDFYAIFKISELLGS"
     gene            complement(1783..3180)
                     /locus_tag="VFJ67_01710"
     CDS             complement(1783..3180)
                     /locus_tag="VFJ67_01710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7289125.1"
                     /translation="MTGKVAILTEDPSLVTEIENIIRSSLVEVFSRTEDIKEADVVLL
                     DADTSGINCLSRLRDKYFVIVITAEKGTRYLIESMTFGAFDCVFRPLKNSSLALSLEK
                     ALGIKSEIKGHLIEFHGGTQGSSVTCAIVGDSPMLQEVCKLIGQVGRVDVPVLITGES
                     GTGKELVAESVWKVSTRWEKPFVVLNCAAIPENLLEAELFGYEKGAFTGASTSRTGKF
                     EEADGGIIFLDEIGDMPLSLQAKVLRVLQSGTFHRLGSSKEISVDVRIITATNKDLEK
                     MVRDGKFREDLYFRINVVRIHLPALKERKQDIPLLAECFTRRHGTQIGKEIKGATSKF
                     ENALMEYDWPGNVRELENVIRKAIAFTKTPYLTSYDLDLRGKQQAMKPAGNVSFKDAL
                     RISVKSLLGSGHGENIYDTVVKEAEVVLLEEALGASGWNRSQAARRLGINRLTLRRKL
                     DEYGIGAKTGPIESD"
     gene            3369..4382
                     /locus_tag="VFJ67_01715"
     CDS             3369..4382
                     /locus_tag="VFJ67_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HET7289126.1"
                     /translation="MDLVLPIAIVLSAFLALNIGANNSAASMATAYGAGVRTKREATV
                     MIAVFAVLGALLAGAPVVKTLGNGLVPGTMFSTHIGLVIIIFIIAIIFISWANISRVP
                     VATTHAIVCAIVGVGLYSKSLNGEKFFEIVIWWVITPLVALAVNYLIAKHLYLKTLQF
                     LTDRYSEGSINKILSILITLSGVFVAFSAGANNSANSVGPLVGLGLVNANIGAVMAGV
                     AMGVGAILFGGRVLESVGKGITEICILRAVSVEFTGAAIILTASLFGVPVSLAEIVTA
                     GIIGFSLAQQGFSVTAQNRHVVRIAFFWFVIPFVAVAMGYFMTSLYFSYDVAAMISRN
                     FGH"
     gene            4436..5212
                     /locus_tag="VFJ67_01720"
     CDS             4436..5212
                     /locus_tag="VFJ67_01720"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET7289127.1"
                     /translation="MSGSTSKKTRGAEARKRIFSPDEVDELNLKFESSGADEVLKWAF
                     DNLHPRVALASSFQAQGMVLIDMLMSISPEARIFTLDTGRLNQETYDVMDEVRKRYGA
                     RIEVVFPERAEVEEMVLERGVNLFYKGKDNRILCCEIRKVRPLKRYLAGLDGWVTSIR
                     RDQLETRAEAQKFELDEGNSGVLKINPLVDWTSKMVWDYIKERKVPYNKLHDMGYPSI
                     GCAPCTRAVKPGEDERAGRWWWESDSDKECGLHFNHNGDS"
     gene            complement(5242..5682)
                     /locus_tag="VFJ67_01725"
     CDS             complement(5242..5682)
                     /locus_tag="VFJ67_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7289128.1"
                     /translation="MPVKSERDLVLEANERFYKALGARDLTLMDTVFVRDERAGCTHP
                     GWIMLRGWEAIRQSWENIFDPEDKLSIKLHNVTVDIKGDAACVTCIQELTYINRAPVT
                     MNVSVSTNLFEKTESGWLMVIHHASPVPFVTEEASPEKESNNLQ"
     gene            complement(5787..6710)
                     /locus_tag="VFJ67_01730"
     CDS             complement(5787..6710)
                     /locus_tag="VFJ67_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7289129.1"
                     /translation="MSFLRLGKDNWARTRPTKEGLTFIALSLFVGFAAINTGNNLLYL
                     TFGIMMSFVVASGVLSMINLSRIEVRLKPHGDIFALTPAALRFSLTNSKSLIPSYSLN
                     IEIGEKKTFIPYLPPGVESQAPLRHVFRERGWNKIPEARLTTRFPFGFFTKWIKIDLG
                     AEEVLVFPKVEAAGADSGTFKERPGDAESDKTGFGHDLRSIKEFRQGDNPRLIHWKTT
                     AKTGRLMVREMEDEETRGAVIRFIPERDGSRLEHQISRLASLHVELLKEGYEVEFVTP
                     DGTFSSARSGGSPRPVLRYLALFRGDEKVRA"
     gene            complement(6800..>7977)
                     /gene="rseP"
                     /locus_tag="VFJ67_01735"
     CDS             complement(6800..>7977)
                     /gene="rseP"
                     /locus_tag="VFJ67_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET7289130.1"
                     /translation="ARAQSLAGADAYSEKEYPRGFSNQPLFNRLLIVVAGPFMNFLLP
                     FIFLPIVFMIGITVPAYLEQPPVIGYIKPGSQAEEAGFMKGDRIIEIEGAKVSDWKDV
                     NIELQTNPDALLNIEVERDGAKKVIPVKATASSEGIVAIGFGEPIEPKIGSVIAGTPA
                     DEADLRKGDEILDINGTKIADWDQMASIVKENSGKEITLLVNRDGKEITKKITPETSV
                     ETGKGAIGVSPYQEEIVKKYGFFGAIVNGVKEAANMIVEVVVLLFGFLFKLVTGKIAL
                     GTAGKSLAGPILIAKVSGAAAENGIAQLLQFTCFISINLAIINLFPIPMLDGGHVLYL
                     VIESIKKKPLSQRSLEISQRIGLTILIFIMFLAIYNDLSRVKGDILDSLSRVVNVVK"
BASE COUNT         1753 a         2093 c         2196 g         1935 t
ORIGIN      
        1 cggagctcat aaagacctct ctcgtccctg atttctacga gcttactccc gagaagttca
       61 acaataagac gaacggaatc actcagagaa ggtggctcct tgactgtaac ccccgtcttt
      121 caagtctcat aaacaggaca gtcggcgacg gctggataag agacctgtac aagctgagac
      181 ccctcgagaa ttattcgcag gatgagggtt ttcaggggga gttcatgcgg gtcaagcaga
      241 tcaacaagga aaatctcgca aggctcatac ataccattac ccggatcacc gtagacccgg
      301 agagcctgta cgacgtccag ataaagcgga tacacgagta taaacgtcag cttctcaaca
      361 tcatgcacat tattcaccag tatttcaggg tcgtggagga cggcatcgag ctcaatgtgc
      421 cgaggactta tatcttcgcc ggcaaagccg caccgggata ctgggctgcg aagcagataa
      481 tcaagctgat aaataacgta gccgatgtcg taaactccga cccgagggtg ggcggccaga
      541 tcagggtgat tttcatgccc gattacaaag tgtcgctcgc ggaaaagata atacccgcgg
      601 ccgatctcag cgagcagatt tccaccgccg ggaaggaagc gtcgggcacg ggcaacatga
      661 agctcgcgct gaacgggtcg ctcactatcg gcacgatgga cggggcaaac atagagataa
      721 gggaagaggt gggcggggag aatattttca tattcggtct gagggcccac gagatagcgg
      781 agatgaaaaa gacgcatagt tatcatccga gggagatttg tgaaaagaac ccgtatctca
      841 agagggtaat ggattcgttc gaatcggatg tattctgcaa ggaagagcgc gggctgttca
      901 aatggatata tgaagcgctt ctcattaacg gcgacaccta ttttcacatc gcggatttcg
      961 agtcctacgc cgagacgcag aagacggtag ggcaggagta caaaaatacc ggggactggg
     1021 cgaggaaggc gatactcaac gtcgcgcgca tcgggaagtt ctcgagtgac cgcacgatat
     1081 cggaatacgc cgaagatatc tggaaggtag ggccgctgaa agattagccg catgcctttc
     1141 cggggagccc gccgcttcac ttaaggcgtt ccgtcaaatt gacaataaat ctcgtcctag
     1201 gttagattca tacgccgtgg cgtccgaaaa gaaagaaagg ctaaagctca tactgctcgg
     1261 gaactcaatt ctgaggggta agttcacgct cgcgtccgga aaggagagcg actattatat
     1321 cgacgcgagg cttacgaccc tcgatcctga aggggtttct atcgtagcgg atattttcct
     1381 cgaagagatc aggcgcgatc cgctgatcaa aaccgtcggg gggccgacca tgggggctga
     1441 ccctatcgta ggcgctctgc tcggcgagag ccataggggg aattatcctc tgagaggttt
     1501 ccttgtccgg aagcaggaaa aggagcacgg caccggcaag atgatagagg gaaatctcag
     1561 ggccggcgat agtaccgccg ttgtcgagga tgtcgttact tcggggggtt ccgtaattac
     1621 tgcgattaac gcggtgaaga gcgcgggagc gcatgtgagg aaggtacttg tgatagtcga
     1681 ccgcgaagaa ggggcggcgg agaagttccg ggagatgggg atcgactttt acgcgatatt
     1741 caagatttcc gagcttctcg gaagctgacg ccgcgccgca tttcagtcgg attcgatagg
     1801 gcctgtcttg gcgccgatcc cgtactcgtc cagttttctt ctcagagtaa gcctgttgat
     1861 gcccagtctc ctagcggcct gggaacggtt ccatccgctc gcgcccaggg cttcctcgag
     1921 caaaaccacc tctgcttcct tgacgacggt atcgtatatg ttttccccgt gtcccgaccc
     1981 gagcaggctt ttgacggata tccttagcgc gtctttgaac gatacgttcc ctgcgggctt
     2041 cattgcctgc tgctttcccc tgaggtcgag gtcgtatgag gtgagataag gcgtcttcgt
     2101 gaacgcgata gcctttctta tcacgttctc gagctccctc acgttgcccg gccagtcgta
     2161 ctccatgagc gcgttctcga acttgctggt cgcgcctttt atttcctttc ctatttgcgt
     2221 gccgtgtctt cgcgtgaagc actcggcgag gaggggtatg tcctgttttc tctccttgag
     2281 agccggcagg tgtatcctga cgacgttaat acggaaatag aggtcttccc tgaatttccc
     2341 atccctaacc atcttctcga gatccttgtt cgtcgccgtt atgatcctga cgtcgaccga
     2401 tatctccttg ctcgatccca gtctgtggaa cgtcccgctc tggagcaccc tcagcacctt
     2461 agcctggagc gataacggca tatcaccgat ctcatcgagg aatattatgc ccccgtcggc
     2521 ttcctcgaat ttacctgtgc gggacgtgga agcccccgta aacgcgcctt tctcgtagcc
     2581 gaagagctcc gcctccaaaa ggttctccgg gatcgcggcg cagttaagca ccacgaaggg
     2641 cttttcccac ctcgtgctga ccttccatac tgattcggcg acgagctcct ttcccgtccc
     2701 gctttcaccc gttataagca ccgggacgtc cactcttccg acctgcccga tgagtttgca
     2761 aacctcctgg agcatggggg agtctcccac aatcgcgcac gtgacgcttg agccctgggt
     2821 gccgccgtgg aattcgatca ggtgtccttt gatctcggac tttatgccca atgccttctc
     2881 cagcgatagc gcgagggacg agtttttaag gggtctgaat acgcagtcga acgcgccgaa
     2941 tgtcatcgac tcgatcaggt acctcgtgcc cttttcggca gtaattacga ttacaaaata
     3001 tttatccctc aggcggctga gacaatttat tcccgaagta tcggcgtcca ggaggacgac
     3061 atccgcttcc tttatgtcct ctgttcgaga aaagacctcg acaagactgc ttcttatgat
     3121 attttctatt tctgttacga ggcttgggtc ctcggtcaaa attgcgactt tgcctgtcat
     3181 gtatacactc tccgtcgtga ttgtaacaaa aatatacgga gtgattatcc tctaatcaag
     3241 ttgacgttat gttagggtac aatatagcct ttaattacac gattatttat tatggcactt
     3301 aatttgcatc gatttatagg attcggcaat caaaaactta ggttgttaat tcgcacggag
     3361 actcctcttt ggatttagtc cttcctattg ctattgttct gtccgcattc ctcgcgctga
     3421 atataggtgc gaataattca gccgcctcga tggccacggc ttacggcgca ggcgtaagga
     3481 cgaaaagaga agctaccgtc atgattgccg tttttgcggt tctcggcgcg cttttggccg
     3541 gcgccccggt cgtcaagacc ctcgggaacg gcctcgtgcc gggcacgatg ttctcgactc
     3601 atataggcct cgtaatcatt atctttatca tcgcaattat ttttatttcc tgggcgaata
     3661 tatcacgcgt cccggtggca actactcacg ccatagtatg cgccatagtg ggggtcgggc
     3721 tttactcgaa atcgctgaac ggcgaaaaat ttttcgagat cgtgatatgg tgggtaataa
     3781 cccctcttgt ggcgctggcg gtgaattacc tcatcgccaa acatttgtat ctgaaaacat
     3841 tacagtttct cacggacagg tattcggagg gaagtataaa taagatactt tcgatcttga
     3901 taacgctgtc gggcgtgttc gtggcctttt ccgcgggcgc taataattcg gctaattccg
     3961 tcggcccgct tgtgggtctc gggctggtaa acgccaatat tggcgccgtc atggccggcg
     4021 tggcgatggg agtgggggcc attctcttcg gcgggagggt gctcgaaagt gtcggaaagg
     4081 ggattacgga gatatgcata ttgagggcgg tctcggtcga gtttacaggg gccgccataa
     4141 ttctcaccgc atcgctcttc ggcgttccgg tatcccttgc cgagatagtg acggcgggaa
     4201 taatcgggtt ctccctggca caacagggct tttcggtcac ggcacagaac cgccacgtcg
     4261 tgagaatagc gtttttctgg ttcgtcatac cttttgtagc ggtggctatg gggtatttca
     4321 tgacttccct ttatttcagc tacgatgtcg ccgcgatgat aagcaggaat ttcggtcatt
     4381 aggatatata atagggttga gcataacgaa gcaggagagg tgattaaaaa tcagattgtc
     4441 aggatccaca agtaaaaaaa cgcggggcgc ggaggcccgg aaacggatat tcagcccgga
     4501 cgaggtagat gaattaaacc tcaaattcga gagcagcggc gctgatgaag tgctcaaatg
     4561 ggcgttcgac aacctgcatc cgagagtcgc tctggcctcg agcttccagg cccagggcat
     4621 ggtgctcata gatatgctca tgtcgataag cccggaagcg aggatattca ctctcgatac
     4681 gggcaggctc aatcaggaga cctacgacgt tatggacgag gtgagaaaaa ggtacggcgc
     4741 gcggatagag gtcgttttcc ccgagagggc ggaggtcgag gaaatggttc tcgagcgcgg
     4801 ggtaaatctg ttctataaag ggaaggacaa caggatactc tgctgcgaaa taagaaaggt
     4861 gaggccgctc aagcgctatc tggcgggcct agacggctgg gtcacgtcca taaggagaga
     4921 ccagctcgaa acgagggccg aggctcagaa gttcgaattg gacgagggga acagcggcgt
     4981 actgaaaata aatccgctcg tcgactggac gagcaaaatg gtgtgggatt acataaagga
     5041 acgcaaggtg ccctacaata agctccacga catgggctac ccgagcatag gctgcgcccc
     5101 gtgcacgcgc gccgtaaaac ccggcgaaga cgagagggca gggcgctggt ggtgggagag
     5161 tgattcggac aaggagtgcg gtctccactt caaccacaac ggggattcgt gagatagatc
     5221 caatctcatg attaaataat ttcactgcaa attgttgctc tctttctcgg ggcttgcctc
     5281 ttccgtgacg aagggtacgg gcgaggcgtg gtgtattacc atgagccagc cggattcggt
     5341 tttttcaaag aggttcgtgg agacggagac gttcatcgtt acaggggctc tgttaatata
     5401 ggtgagctcc tggatgcagg tgacgcaagc ggcgtcccct ttgatgtcga ccgtgacgtt
     5461 gtggagtttt atggagagct tgtcttcggg gtcgaagata ttctcccagc tctgccttat
     5521 ggcttcccat cccctgagca ttatccagcc gggatgcgtg catcccgccc gctcgtccct
     5581 gacgaacacg gtgtccatga gagtgaggtc cctcgcgccg agcgccttat aaaacctttc
     5641 gttcgcttcg agaacgaggt ccctttcgga tttaaccggc atacgaattt atacctgaaa
     5701 atagaaggaa agtcttattg ccgcttgccc gagggctctc cattctcggt acgaagaaaa
     5761 ttgttcaccg tccgcatcga acgaccctac gcccggacct tttcgtctcc cctgaatagg
     5821 gctaggtatc tcagcacagg cctcggcgag cctcccgacc gggcgcttga gaaagtcccg
     5881 tccggggtta cgaactcgac ctcgtagcct tctttcaata actctacgtg gagcgacgcg
     5941 aggcggctta tctggtgctc gagcctggac ccgtcccttt ctgggatgaa tcttatgacc
     6001 gctcccctcg tttcctcgtc ctccatctcc cttaccatga gcctcccggt cttggccgtt
     6061 gttttccagt gtatgagcct cgggttgtcg ccctgccgga attcctttat cgacctgagg
     6121 tcgtggccga atcccgtctt atccgactcg gcatcacccg gtctctcttt aaacgtcccg
     6181 ctgtccgcgc cggccgcttc gactttaggg aataccagca cctcttcggc gccgaggtct
     6241 atctttatcc atttcgtgaa gaacccgaaa gggaaccttg tcgtgagcct ggcttcggga
     6301 atcttgttcc aacccctctc cctgaatacg tgcctgagtg gagcttgaga ctcgaccccg
     6361 ggcggcaggt agggaatgaa agttttcttc tcgccgattt cgatattgag ggagtacgag
     6421 ggtatgaggg acttggagtt cgttaaagag aatctgaggg cggccggggt gagcgcgaaa
     6481 atatcaccgt ggggttttaa tcgcacctct atcctcgaga ggtttatcat agacagaacc
     6541 cccgaggcca cgacgaagct catcatgatg ccgaatgtga ggtagaggag gttgttcccc
     6601 gtgttgattg ccgcgaagcc gacgaagagg ctcaaggcga tgaacgtcag cccttctttc
     6661 gtgggcctcg tccttgccca gttatccttt ccaagccgaa ggaagctcat attttaaggg
     6721 tcggtgcggg tccggtaagc taagccgtcc acacgcttct tcaggaatta tatcccttat
     6781 cgcgcgccgg ggggggcggt catttcacga cattgacgac ccggctgagg gagtcgagaa
     6841 tgtctccctt gactctcgac aggtcgttat agatcgcaag gaacataatg aatatcagta
     6901 tcgtcaggcc tatcctctgg cttatctcta gggacctctg gctgagaggc tttttcttta
     6961 tgctctctat cacgagataa agcacgtggc ctccgtcgag catcgggatc gggaacaggt
     7021 tgattatcgc gaggttgatg ctgatgaagc acgtgaactg aaggagctgc gctatgccgt
     7081 tctccgcggc ggcccccgag accttcgcta taagtatcgg gcccgcgagg ctcttaccgg
     7141 ccgtgccgag cgctatcttt cccgtgacca gcttgaagag gaagccaaac aggagcacca
     7201 ccacttcgac tatcatgttg gcggcctctt tcacgccgtt aacgatggct ccgaagaaac
     7261 cgtatttctt tacgatctcc tcctgatatg gtgaaacgcc tatcgcgccc tttccggtct
     7321 cgaccgaggt ttcaggcgta attttcttcg ttatctcttt gccgtccctg tttacgagaa
     7381 gcgtgatctc tttcccggaa ttttctttaa ctatcgacgc catctggtcc cagtcggcga
     7441 tcttcgttcc gtttatatcc agtatctcat cccctttccg gaggtcggct tcgtcggccg
     7501 gcgttccggc gatgacggac ccgatcttgg gctctatagg ctccccgaac cctattgcga
     7561 caatcccttc cgaggaggcc gtcgccttca ccggaattac cttcttcgcc ccgtccctct
     7621 caacttctat attgaggagg gcgtcggggt tggtctggag ctcgatattg acgtccttcc
     7681 agtcggagac ttttgcacct tcgatttcaa ttatcctgtc gcctttcatg aaccccgcct
     7741 cttcggcctg cgagccgggt tttatataac cgatgacggg gggttgttcg agatacgccg
     7801 gcacggttat ccctatcatg aaaacgatcg ggaggaatat gaaaggcagg aggaagttca
     7861 tgaacggacc cgccactact attaagagcc tgttgaacag aggctggttc gagaaccccc
     7921 tcgggtattc cttctccgag taggcgtccg cccctgcgag gctctgcgcc ctggccg
//
LOCUS       DASSJH010000082         6749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_237075, whole genome shotgun sequence.
ACCESSION   DASSJH010000082 DASSJH010000000
VERSION     DASSJH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237075"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1597
                     /locus_tag="VFJ67_01740"
     CDS             290..1597
                     /locus_tag="VFJ67_01740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET7289131.1"
                     /translation="MNLSVIGTGYVGLVTGACLAGSGNNVICVDIDEEKVKSLKKGHV
                     PFYEPGLEEIVKKNLKGKRLHFTTDIGNAVKSSFIILIAVGTPPNHDGSANMDYVLNA
                     VRSIGGHMDDYKIIVTKSTVPVGTTEMIRDEIRKLTDIQFDVASNPEFLKEGNAVEDF
                     MKPDRVIIGVDKPAVGGILKELYSPFMRSGDRAIVVSIRSSELAKYTANAMLATRISF
                     MNDIANLCELVGADISEIRAAIGSDGRIGRHFLFPGIGYGGSCFPKDVKALISTADAL
                     GYDLKVCRATDEVNTRQRELFWDKIAGYYKGGLKGKRIGVWGLSFKPETDDIREAPSL
                     YIIDRLIGSGAEVVVHDPVAMENARQRFGDIVHYAKSNYDACRGVYGLVINTEWNEYR
                     QPDFVKMKELMKDCVIFDGRNLYDPVKLEKHGFTYFGVGISTT"
     gene            1924..2514
                     /locus_tag="VFJ67_01745"
     CDS             1924..2514
                     /locus_tag="VFJ67_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289132.1"
                     /translation="MGSITGLTRIKFLFYLLFIPLLTTFSFGSFLKAQDEESVLEAEE
                     GEEAAPPAPASTPAAKEKSVIAETDGEYPDVRLEVTELKRTSGDTLTLKFTIINDSSE
                     DMDFSYNFVEQGKYENHDFATVGGVNLVDAAGKKKYLVLRDSENKCLCSRNLKTVKPG
                     SSAKLYAKFPAPPESVEKITVEIPHFIPMDDVPISK"
     gene            2606..3166
                     /locus_tag="VFJ67_01750"
     CDS             2606..3166
                     /locus_tag="VFJ67_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7289133.1"
                     /translation="MPNLFYPIRRGKLLLVLGIFVPAVLFPYVAGAQQDIQSRVLDII
                     FRVEDLKGETLDLQMEETETEIKIELSGDILFDFDKWDIRPEAEPVLTQVAEVINKYP
                     KAVVLIEGFTDSKGSDSYNLKLSGKRAASVKDWLLKKGDVGSKKITTKGLGEADPVAP
                     NENPDGSDNPEGRQKNRRVEITVKKG"
     gene            3184..4278
                     /locus_tag="VFJ67_01755"
     CDS             3184..4278
                     /locus_tag="VFJ67_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289134.1"
                     /translation="MDNDGGKEFKHGEQFILSILGLVCILLGSVFFIQRSESISSLAL
                     AQTRPAASTAKKKAEEFTEQRKNASQGQWLLYIGNPVLRRGKIDSWDDFKVGSPVVLK
                     EGDLFRMWYRGCHFIGDEFTCGVGHAVSEDGVIWKKSPGPVLVIEDTSRSGLLDTITL
                     LYLPEKGYMMWYSLSSDWFAGRHYATINLAVSEDGLNWRQEGAVLRAVRKGTTISPSA
                     FYDGKVFHLWYVDGTSAEKPKALMHVTSPDGRKWQVAGWTFLNTLGVNPGRLWILSEK
                     SGGYRGIFAHRPDENREKGFLGQLVSADGNIWEMGKVINTDPDAPEKGVIQDAPSALP
                     EPNGLWVWFVMRPEDGAETINAAYGKGKAP"
     gene            4332..6494
                     /locus_tag="VFJ67_01760"
     CDS             4332..6494
                     /locus_tag="VFJ67_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289135.1"
                     /translation="MSVSVSAQQPSRPNRIVWVGIEEFREVSIKPGRINPASGDANEH
                     YKNIRIKFRYVEERDLEGKGSFTSRHVEWTARESHIVHGIISTECKGEGSLELGPGDM
                     ITDEQMKRMQMTCKEEHEGAIFGYFWLPQLIDPPDVVEWEMLRDDCSYSEERPGYRYS
                     VWVSPEIDAVMKVETGSNSEYKQFVPRPGEQITFTVRSNVPARFRFRLEDVSHFAGFA
                     TNADIKPYFFHIYGLDHLEGKYETKKQGEPLKVDPDLIFDPKLYEDRAEWEQPTYETV
                     TTAKESASASVTVTAMDYAAYGRLKTEVKGKCGGWEPVRILVGGEEKEFVTIPLDEND
                     NLMSDKMDEPQNGITDWGYDGDAGRDDDRYPQGDGTPGDGFTAFEEYRGFMTQGANFA
                     GGCDDRGYDLQIRTDPGRKDLFIYSPDPELAGLALRFENSTKGAEDLKGIGVHLICEP
                     HSVARKVNYTLSFWGPKEWRGKTLFGGDQHGLNLLDASLEDGTLGLTHGTTGLGPPKD
                     VWGDVKIDKNKIFSMGDRPYFNAPLELAKTVLHELGHAVGIDHHGDSNVYLALLITDK
                     PCPGVISDPLVILGDGISVTSPPLSAGERACVIFNVARRHAQNSGNQACPMKYTYWNS
                     YESPSSPLQPMGDIKLTDSQRRHATLKGYRGEIHEYRRDLDTPSLGPFCDKATGTGIN
                     GYPGEANHAGDSCRVCAGQIRVKDWDPSPHTPPACPAD"
     gene            6516..>6749
                     /locus_tag="VFJ67_01765"
     CDS             6516..>6749
                     /locus_tag="VFJ67_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289136.1"
                     /translation="MYKALFRITLFTMVVIAGYMDRNAWKFPALAAKVPDVSLSLRVN
                     GVREVSLLPGAPLIFAVFMSGSKTDPPFHIGGSG"
BASE COUNT         1713 a         1628 c         1890 g         1518 t
ORIGIN      
        1 gccgacgagc tctatataag cgaaaagacc gtaaagtttc acctctacaa ggtattcaag
       61 aagctcaagg tgaaaaacag gtcggagctc atacttttcg gcttccggca cgggctcatc
      121 agctgaacgg tgccgcttga aggcgcggta tcccggttgc ctgattgcgc cagtccttga
      181 ttaactcccg aattgggact aaattaaagc tctgttcgtc ggatgaataa acaaatccca
      241 gtaaacggtt tatgaatttc atcccgtcaa taatcatgga gtgtccgtaa tgaatctgag
      301 cgtgatcggc acaggctacg tgggtctcgt caccggggct tgccttgcgg ggagcggaaa
      361 caacgtcatt tgcgtcgaca tagacgagga gaaggtaaag agcctcaaga aagggcacgt
      421 gcccttctac gagcccggcc tcgaggagat cgtaaaaaag aacctcaagg gaaaacgcct
      481 ccacttcacg accgacatcg gaaacgcggt gaagagctcc ttcatcatcc tgatcgccgt
      541 cggcaccccg cccaaccacg acggctcggc gaatatggat tacgtgctca acgcggtcag
      601 atcgataggc gggcacatgg acgattacaa gataatcgtt acgaagagca ccgtccccgt
      661 cgggactacg gagatgatac gcgacgagat aaggaaactg accgacatcc agttcgacgt
      721 tgcgtcaaac ccggagtttc tcaaggaagg taacgccgtc gaggatttca tgaagcccga
      781 cagggtgata atcggcgtcg acaagccggc tgtgggaggg atactgaaag agctttattc
      841 gccattcatg cgctcgggcg acagggccat agtcgtatcc atcaggtcgt ccgagcttgc
      901 gaaatacacc gcgaacgcca tgctagcgac gcgcatctcg ttcatgaacg acatcgccaa
      961 cctctgcgag ctcgtagggg ccgacatatc cgagataagg gcggccatag gctctgacgg
     1021 aaggatagga cgccatttcc tcttccccgg aatcggttac gggggctcct gtttccccaa
     1081 ggacgttaag gcgctcataa gcacggccga cgcgctcgga tacgacctca aggtgtgcag
     1141 ggcgaccgac gaggtcaaca cgaggcagag ggagctcttc tgggacaaga tcgcgggtta
     1201 ctacaagggc ggtctcaaag gaaaaagaat aggggtctgg gggttgtcgt tcaaacccga
     1261 aacggacgat ataagggagg ccccttcact ttacatcata gacaggctca tcgggagcgg
     1321 cgccgaagtc gtcgtccacg acccggtcgc catggaaaac gcgaggcagc gcttcggcga
     1381 tattgtgcat tacgctaagt cgaactacga tgcgtgcagg ggagtttacg gcctcgtgat
     1441 aaatacagag tggaatgagt acaggcagcc cgatttcgtg aagatgaaag agttgatgaa
     1501 ggactgcgtg atattcgacg gcaggaacct ctacgatccg gtgaagctcg aaaagcacgg
     1561 gtttacatac ttcggcgtag ggattagtac gacctgatag ggaaaaacgt agatgctttc
     1621 gaattaaagt ttcttcaata ttaggcatcc cataaacaaa tagtgcggct caatctcaag
     1681 taaccgtata cttcggccgg tgtatgaacg ctggctggtc catttccgaa catatggcct
     1741 aatcccgtta ttgttcccct aatctcgaca tcattgagag gtagttattt tgcaggcaag
     1801 tcgtaactac ctgataaagc ggtgtaattc tgagagatct catccgggtg aaatagccga
     1861 ttgacaaatt aaatcctatt ttgctatgat atggggaagt taacttatgt gggagggggc
     1921 atcatgggct caataaccgg tttgactcgg attaagtttc tcttttatct gttatttatt
     1981 cctctactaa cgactttttc ttttggtagc tttctaaagg cacaggacga ggaaagcgtt
     2041 ctcgaggcgg aagaaggcga ggaagccgcc ccgcccgctc ctgcttcaac gccggctgcc
     2101 aaggagaagt ctgttatagc cgagaccgac ggggaatatc cggacgtaag gcttgaagtc
     2161 acggagctca agcgcacgag cggcgatacg ctcacattaa aatttacgat tattaatgat
     2221 tcgtccgagg atatggattt cagttacaac ttcgtcgagc aagggaaata cgaaaaccat
     2281 gattttgcca cggtaggcgg ggtgaatctg gttgatgcgg ccggtaagaa gaaatacctg
     2341 gtgctgcgcg attcggagaa caaatgcctg tgcagccgta atttaaaaac cgtcaaaccc
     2401 gggtcgagcg cgaagttgta tgccaagttt ccagcccctc ccgaaagcgt ggaaaagata
     2461 accgtagaga tacctcattt tatccccatg gatgacgtcc ctatcagcaa ataggcaagc
     2521 ctgaattcgg aaattccggc ggccgtcatt tggaattatc tcttaaaagg ttgggttaat
     2581 caggtatcaa tccggggagg ttatcatgcc gaatttattt tacccgataa gaagaggcaa
     2641 gctcttactc gttcttggta tttttgttcc cgcagtcctt tttccttatg tcgcgggagc
     2701 ccagcaagac atccaatcgc gggttctgga tattattttc agagtcgaag acctgaaagg
     2761 ggaaaccctt gatttgcaga tggaagagac tgaaaccgag ataaagatcg agctttcggg
     2821 agacatcctg tttgatttcg ataaatggga cattcgtcct gaggcggagc ccgtgcttac
     2881 acaggttgca gaggttatca acaaatatcc caaggccgtg gttttgatag agggttttac
     2941 tgattcgaag ggttcggatt cctataatct caagctttcc ggcaagcgcg ccgcatccgt
     3001 gaaagactgg ctcctgaaaa agggcgacgt cggcagcaag aagataacta caaagggcct
     3061 tggtgaagcg gacccggtcg cccccaacga gaatcccgac gggagcgaca acccggaagg
     3121 tcgccagaaa aaccgcaggg tggagataac tgtgaagaag gggtagtcag agagaatgag
     3181 ttgatggaca acgatggcgg aaaggagttt aagcatggcg aacagtttat tctctccatc
     3241 cttggtcttg tatgtatttt gttaggatcg gttttcttta tacaacgttc tgaatctata
     3301 tccagcctcg ccttggcgca aacccggcct gccgcctcca cagcgaagaa gaaagccgaa
     3361 gagtttaccg aacaacgcaa aaatgcttct caagggcaat ggttattgta tataggcaat
     3421 ccggtccttc gccggggaaa gattgattcc tgggacgact tcaaagtcgg ctctcccgta
     3481 gttctcaagg aaggcgatct cttcaggatg tggtataggg gctgtcactt tataggagac
     3541 gaatttacct gcggcgtggg acacgcagtc tcggaggacg gggtgatctg gaaaaaatct
     3601 cccgggcccg tgctcgtgat cgaagacaca tcccgcagcg gacttctcga cacgataact
     3661 cttctgtatc tgccggaaaa aggttatatg atgtggtatt cgctaagcag cgactggttc
     3721 gccggacgtc attacgcaac gatcaacctt gccgtatccg aagatggatt aaattggcgg
     3781 caggaaggtg ccgttttgag agcggtcaga aagggaacga ctatttcccc ctcggccttt
     3841 tacgacggca aggtttttca tttatggtac gtcgatggca cctctgcgga aaagccgaag
     3901 gctctcatgc atgtcacctc tccggacggc agaaagtggc aggtagcagg ctggactttt
     3961 cttaacacgc taggtgttaa tccgggcagg ttgtggattc tgtccgaaaa gagcggcggt
     4021 taccgcggca tatttgctca tagaccggat gaaaatcgag aaaaaggttt tctgggccag
     4081 ctcgtctcgg ccgatggaaa catctgggaa atgggcaaag tgataaatac agatccagac
     4141 gcccctgaaa aaggtgtcat acaggatgcc ccctcggcgc tccctgagcc taacgggctt
     4201 tgggtgtggt tcgtaatgcg gcctgaagac ggcgcagaga cgatcaatgc cgcctacgga
     4261 aaagggaagg cgccgtgaat catttccttt atcttctttt gctatgggcg tcatcggcaa
     4321 tcttttttgc tttgtctgtt tccgtttcag cccagcaacc atcgcggcct aacaggatag
     4381 tgtgggtggg cattgaggaa ttcagagaag tttccatcaa acccgggagg attaatcccg
     4441 catcggggga tgcaaacgaa cattataaaa acatccgcat caagtttcgt tacgtcgagg
     4501 agagagactt ggaggggaag gggagcttta cttcccgtca cgtcgagtgg acagcaagag
     4561 aatcgcatat cgtccacggc attataagca ccgaatgtaa gggagaagga agccttgagc
     4621 tcggccctgg ggatatgatc actgacgagc aaatgaaacg aatgcaaatg acttgtaaag
     4681 aggagcatga gggtgctatt ttcggctact tctggctgcc tcaactgatc gatccccccg
     4741 atgtggtgga atgggaaatg ctccgtgacg actgttcata tagtgaggag cgtccgggtt
     4801 accgctacag tgtttgggtc tccccggaga ttgacgcagt tatgaaggtt gagacgggca
     4861 gtaacagtga gtataagcaa tttgttccaa ggccggggga gcaaatcaca ttcactgtac
     4921 gttcgaatgt acctgctcgc ttccggttca ggctcgaaga cgtctcacat tttgccggtt
     4981 tcgccacgaa cgccgatatt aaaccctatt tctttcatat ctatggactg gatcatcttg
     5041 aggggaaata cgagacaaaa aaacagggtg agccgctaaa ggtagatccc gacctcatct
     5101 tcgatcccaa gttatatgaa gaccgtgccg agtgggagca gccgacgtat gaaactgtga
     5161 cgaccgcaaa ggaatctgca agcgcgagcg ttacggttac ggcaatggac tatgccgcct
     5221 atggtcgcct caagactgaa gtcaaaggaa aatgcggggg gtgggagccg gttcgtatcc
     5281 tggtcggagg ggaggaaaaa gagttcgtta caatccctct cgatgagaac gataacctga
     5341 tgtcggataa gatggacgag ccgcaaaacg gcataacgga ttggggatat gatggcgacg
     5401 caggccgcga cgatgaccgc tatccgcagg gcgacggaac gccgggcgac ggtttcacgg
     5461 ccttcgaaga gtatcgtggt tttatgactc agggcgccaa cttcgcaggc ggctgcgatg
     5521 accgcggcta tgacctgcaa atacgtaccg atcccggccg gaaggacctc tttatctatt
     5581 ccccggaccc ggagctggcg ggtctcgcgc tcaggtttga gaacagcaca aaaggggcgg
     5641 aggatttgaa agggatcggg gtgcatctta tctgcgagcc gcactccgtt gcccggaagg
     5701 tcaattatac ccttagcttt tgggggccca aggaatggcg cgggaaaacg cttttcgggg
     5761 gtgaccagca tgggcttaac ctcctggatg cgtcgctgga ggacggcacg ctgggactga
     5821 ctcacggaac tacaggactc ggtccgccga aagatgtgtg gggcgacgtc aagatagaca
     5881 agaacaagat tttcagcatg ggggatcgtc cgtatttcaa tgcaccactt gagctcgcca
     5941 aaactgtctt acacgagctc ggccatgccg taggcatcga ccatcatgga gacagcaatg
     6001 tatatcttgc gctgcttatc actgataaac cgtgccccgg agttatctcc gaccctttgg
     6061 ttatattggg tgacggaatt agtgtgacgt cccctccgct ttcagccggg gaaagagcct
     6121 gcgtcatatt caatgtggct cgccgccacg cacagaacag cggaaatcag gcgtgtccga
     6181 tgaaatacac atattggaac tcttacgagt ctccgagcag cccgcttcag cccatgggcg
     6241 atataaagct cacggactct caaaggcgtc acgccacgtt aaaaggttat aggggagaga
     6301 tacacgaata ccgtagagat ttggatacgc ccagcctggg tcctttttgc gataaagcga
     6361 caggtacggg gatcaacggc tatccgggag aggcgaacca tgcgggagat tcctgccgtg
     6421 tttgcgccgg gcaaatccga gtcaaggact gggatccatc gccgcacacg ccccctgcct
     6481 gccccgccga ttaatggagg aatttggaaa aataaatgta taaggctctt tttcgcataa
     6541 cccttttcac catggtcgtc atagctggat acatggatcg taatgcctgg aaatttcccg
     6601 ccttggcggc taaagttcct gatgtatcgc tgtctctccg ggtgaacgga gtgcgggagg
     6661 tctctctcct tccgggcgcg cccctcattt tcgctgtctt catgagcggg tccaagaccg
     6721 accctccctt tcatatcggc ggttccggt
//
LOCUS       DASSJH010000083         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_883720, whole genome shotgun sequence.
ACCESSION   DASSJH010000083 DASSJH010000000
VERSION     DASSJH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883720"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFJ67_01770"
     CDS             <1..259
                     /locus_tag="VFJ67_01770"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675716.1"
                     /note="involved in lysine biosynthesis; DAP epimerase;
                     produces DL-diaminopimelate from LL-diaminopimelate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET7289137.1"
                     /translation="KVEIMIWERGAGYTLASGSSSCAVAAACVKNGLTGRTVTVSMPG
                     GELVIEIREDWSIRMRGAVEEVAYGTLSDDLIERIKTLGAE"
     gene            323..1573
                     /locus_tag="VFJ67_01775"
     CDS             323..1573
                     /locus_tag="VFJ67_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7289138.1"
                     /translation="MGKLLLSAETVLPVSSKPLSNSSVLVSRGKVRDVGKSAALRKRY
                     KGASEMDLGKGILLPGLINAHTHLELGWIFERLRSFRGFTGWLGQIVREKKEGIPEGD
                     IIRSVEEGINTVIRSGVTTVGEISSYGGIDKPLLKNSGLRTVLFREILDSNEVKTDFS
                     ALEKVTLFEERLFPHSPYSCSPGVLKKALRSHRRNGVPLGIHLAESADEAGFVRGEEN
                     GIERKIFPLIGKAPFKRPRAESPVAYLRNAGLLEGTRITLVHMVQVSGEEARELREQD
                     AALVLCPRSNFFLQVGAPPVMEYAKFKRIGIGTDGLSSNYNLDFFEEMRFLHMLLSVS
                     LGQRAAFVTVYAATLGGAGALYLEDRIGSIEPGKEADLLFLKKGAGAGDPYLSVISSG
                     ARDVGMVMVGGEIIHRALGTYPGI"
     gene            1576..>2195
                     /locus_tag="VFJ67_01780"
     CDS             1576..>2195
                     /locus_tag="VFJ67_01780"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET7289139.1"
                     /translation="MSGVIDRIFVYGTLLRGEERSCYMEGCALDGILEVPGSLYDTGR
                     GYPAALFDDGSDATVSGEIYVMEDPSSKMKELDRVEGTDSGLFRRSRINYSGTEFYCY
                     EAGTLLAESVTAANRIPGGNWRTHSSLAFSDPADFALRFETHIKNSYKTHPEEGTDGI
                     HYVRGNIPILVTAPHATAHVRLGKLKRQEFYTGALAVLLHSLTGCHV"
BASE COUNT          507 a          570 c          688 g          430 t
ORIGIN      
        1 caaggtcgag ataatgatat gggagagggg ggccgggtac acgctcgctt cagggagcag
       61 ctcgtgcgcg gtcgccgcgg cttgtgtgaa gaacggactc acgggcagga cggttaccgt
      121 gtcgatgccc gggggcgagc tcgtgattga aataagggaa gattggtcga taagaatgag
      181 gggtgccgtg gaagaggtcg catacggcac gctgagcgac gatttgatag agaggataaa
      241 gactctcggc gcagagtgat ccctccggta taattacact ccagacgccg ggatgcccgg
      301 tcccggcaaa ttcgaactca ctatgggaaa gcttcttctg tccgcagaaa cggtcctgcc
      361 cgtttcctcc aagcctttga gtaactcgtc cgtgctggtc tcacgcggga aggtaaggga
      421 tgtaggaaaa tcagccgcgc tcaggaaacg ctacaagggt gcgagcgaga tggatctcgg
      481 taaggggata ctgctcccgg gcctcattaa cgcccacaca catctcgagc tcgggtggat
      541 cttcgagagg ctccgcagct tcagggggtt cacgggctgg ctcgggcaga tcgtaagaga
      601 aaaaaaggag ggcattccgg aaggcgacat cataaggtct gtcgaggaag gtatcaatac
      661 ggtcatacgc tcgggcgtta ccacggtggg agaaatttca tcttacggag gcatagataa
      721 accgcttctt aaaaactcgg gcctcaggac cgtgctcttc agggagatac tcgacagcaa
      781 cgaggtaaag accgatttct cggctctcga aaaagtgacg ctcttcgagg aaaggctctt
      841 cccgcattcg ccttattcat gtagtccggg ggtgcttaaa aaagccctcc ggtctcatag
      901 aaggaacggc gtgccgctcg gcattcacct cgccgaaagc gccgatgagg ccgggttcgt
      961 aaggggggag gaaaacggca tcgaaaggaa gattttcccg ctcatcggca aggctccgtt
     1021 caaaaggccg cgcgcggaat cgccagtcgc gtatctcagg aatgcggggc ttctcgaagg
     1081 gaccaggatc acgctcgtcc acatggtgca ggtgagcggg gaagaagccc gtgagctgcg
     1141 ggagcaggac gcggccctgg tactttgtcc gaggagcaat ttctttcttc aggtcggagc
     1201 ccctcccgtg atggaatacg cgaaatttaa aaggatcggt atcgggaccg atggtctctc
     1261 gagcaactac aacctcgact ttttcgagga gatgaggttc ctccacatgc ttctgtcggt
     1321 atctctcggg cagcgtgccg cgttcgtaac cgtctatgcc gctacgctcg gcggcgcggg
     1381 cgcgctctat ctcgaggaca ggataggcag catcgaaccc ggaaaggaag ccgacctgtt
     1441 attcctcaaa aagggagcgg gggcgggcga cccctacctc tcggtcattt cttcgggcgc
     1501 ccgggacgta ggcatggtca tggtcggcgg tgaaataatc cacagggctt tggggacata
     1561 tcccggcata taaatatgtc aggcgttatc gacagaatct tcgtctacgg gacgctgctg
     1621 cgcggggaag agcggagctg ctacatggag ggctgcgcgc tggacgggat tctcgaagtg
     1681 cccggaagtc tgtacgatac gggacgcggt tatccggccg ctttattcga cgacgggtcg
     1741 gacgccaccg tgtcgggcga gatctatgtc atggaagacc cgtcctccaa aatgaaggag
     1801 ctcgacaggg tcgaagggac ggactccggg ctattcagaa ggtcgaggat aaattactca
     1861 ggtaccgagt tctattgcta cgaagcgggg acgctgcttg cggaatcagt gaccgctgcg
     1921 aacaggatac cgggcggaaa ctggaggacg cactcttccc tcgctttctc ggacccggcg
     1981 gacttcgcgc tccggttcga gacgcacata aaaaatagtt ataaaaccca tcctgaagaa
     2041 ggaacggacg gaatacatta tgtgagaggg aatatcccga tactcgtcac ggcccctcac
     2101 gccacagcac acgtacgcct gggaaagttg aaaaggcagg aattctatac gggggcgctc
     2161 gccgtcctcc tccattcact gaccggctgt cacgt
//
LOCUS       DASSJH010000084         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_237103, whole genome shotgun sequence.
ACCESSION   DASSJH010000084 DASSJH010000000
VERSION     DASSJH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237103"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1382
                     /locus_tag="VFJ67_01785"
     CDS             60..1382
                     /locus_tag="VFJ67_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HET7289140.1"
                     /translation="MSLYNLVSFAGIFILMGFAWLISSDRRNVNYRVLIWGVSLQLIF
                     GAFIFLLPAGAKLFLFINDLVVAVLGLASEGAEFLFGRLALPPGTSNAAGEGSLGFFL
                     AFQALPTIIFFSALMSILYYAGVMPRVIKAFSSLFSRLMRLSGAESVSTASNIFVGIE
                     SLLTIKPYLGKMTRSELCTILTAGMATVSSNVLALYIFSLKGVFPTIAAHLVSASILS
                     APASLVMSKLLLPEDSVPETLGISVEPYYDREESLFEAVINGAESGVKLIVGIVALLV
                     AVLGLVALLDLITGGLGAHVNNLTGINIDWSLRGLLGYLFYPFVLVTGVPVADAYQIS
                     KIVGERAVVTEVVSYQDLARAMKEGVLTDPRSPVLAAYALCGFAHVASMAIFVGGAAA
                     LAPGRKKDIASVGLRALIAATLTCLMTECVAGVFYTGTSALLGLDVVP"
     gene            1440..2633
                     /locus_tag="VFJ67_01790"
     CDS             1440..2633
                     /locus_tag="VFJ67_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7289141.1"
                     /translation="MVYKSGSYEAAFDGSDYESGEFLEDAAPSELPGYGFADDAEPEK
                     EEPDEGKGQAPRDPNPELRLINAYFKEVGRVSLLTKREEIELAAKIKECGAYSKRIRK
                     SINRMMGGRPSVCGEPFTDELKALADGLELNGDERERLKKLVLLLEAYTRAETGLKNR
                     FVRANLRLVASLAKKYVGRGIPFLDLIQEGNLGLIKAVERFDHTRGYRFSTYACWWIN
                     QAMIRGIFNQTRTVKIPAYVLEKAGKVWEERAKFVKERGREPHPAEIAKSVEMSVENV
                     KQVLESGNGVCNVRLDSPVWAGEKMTFMDYIPDSVTVPVDSLIAEVSIPEKVESALHV
                     LDEREREIVKMRFGIGFEGAFTLDEIGKRLGLTRERIRQIEKKALSTLRHSDSAPALR
                     SLIEH"
BASE COUNT          596 a          701 c          855 g          624 t
ORIGIN      
        1 ccggacggtc tccccaaggc cgaaagcggg ggttgtgtta tagtgcgagg gaggtaatcg
       61 tgagccttta taacctggtc agtttcgctg gaattttcat actcatgggg ttcgcgtggc
      121 ttatttcgtc cgacaggagg aacgtcaatt acagggtatt gatatgggga gtttcgcttc
      181 agctcatatt cggagcgttc atattcctcc tgcccgcggg agcaaaatta tttttattca
      241 taaatgattt ggtagtggct gtcctggggt tggcctcgga gggagccgag ttcctgtttg
      301 gaaggctcgc gctgcctccg ggaacgagta atgcggccgg ggagggctcg ctcgggttct
      361 ttctcgcttt tcaggcgctg ccgaccataa tcttcttctc cgccctcatg tcgatcctct
      421 actacgcggg cgtcatgccc cgcgtgataa aggctttctc ctcgcttttc tcgagactga
      481 tgaggctctc cggggcggaa tccgtgagca cggcatcgaa tatcttcgtc ggtatagagt
      541 ctcttctgac cataaaaccg tatctcggga agatgacgcg ctccgagctg tgcacgatcc
      601 tgacggccgg catggcgacc gtttcgtcaa acgtgctcgc cctctatatc ttcagcctga
      661 agggagtttt cccgacgata gccgcgcacc tcgtgtcggc gtctatactt tcggccccgg
      721 cttcactcgt tatgtccaag ctgcttcttc ccgaggactc cgtgcccgag acgctcggta
      781 taagcgtcga gccatattat gacagggagg agagcctctt cgaggctgtg ataaacggcg
      841 ctgagagcgg ggtgaagctc atagtgggga tcgtcgcgct cctggtcgcg gtgctcggtc
      901 tcgtagctct cctcgacctg atcacgggag gtctcggggc tcacgtcaat aatctcaccg
      961 gcataaacat cgactggtcg ctacgggggc ttctcggata tctcttttat ccgttcgtac
     1021 tcgtaaccgg tgttccagtg gccgacgcct accaaatatc gaagatagtc ggcgagcggg
     1081 cggtggttac ggaggtcgtt tcctatcagg acctcgcgcg cgctatgaag gaaggggtgc
     1141 tgaccgaccc gcgctccccg gtgctcgcgg cctacgccct ttgcggattc gctcacgtgg
     1201 cttcgatggc gattttcgtg ggcggggcgg cggccctcgc cccaggacgg aaaaaagaca
     1261 ttgcctctgt tggtctgagg gccctcatag ccgccaccct cacctgcctc atgaccgagt
     1321 gcgtcgcggg cgttttctat acggggacat ccgccctcct cggattagac gtagttcctt
     1381 aatagaagcc ccgtcgttat agactgtacg gcttctaata caggctaaga ggaggagaag
     1441 tggtctataa gtcgggatct tacgaggcgg ctttcgacgg ctctgattat gaatcgggtg
     1501 aattccttga ggatgcggct ccgtccgaat tacccggtta cggttttgcg gatgatgctg
     1561 agccggaaaa agaggagccg gacgagggca aggggcaagc gccccgggac ccgaaccccg
     1621 agctcaggct tataaacgct tacttcaagg aagttggcag ggtatcactc cttacaaaga
     1681 gggaggagat cgagctcgcc gccaagataa aggaatgcgg ggcgtattcg aagaggatca
     1741 ggaaatcgat aaaccgaatg atgggcggaa ggccctctgt gtgcggggag ccgttcacgg
     1801 atgagcttaa ggcgctcgcc gacggtctgg aattaaacgg ggacgagaga gagcgtctga
     1861 agaagctcgt gctgcttctc gaagcatata cgagagccga aaccgggctg aaaaaccggt
     1921 ttgtcagggc gaatctcagg ctagtcgcca gcctggcgaa gaaatacgtc ggaagaggta
     1981 tcccattcct cgatctgata caggagggga accttggtct aatcaaggcg gtcgaacgat
     2041 ttgatcacac gaggggatac aggttttcga catacgcgtg ctggtggatt aaccaggcga
     2101 tgatcagggg tatattcaac cagacaagga cggtgaagat accggcctac gttctcgaga
     2161 aggcgggcaa ggtctgggaa gagcgggcaa agttcgtcaa ggagaggggg agggaacctc
     2221 acccggccga gatcgcaaag tctgtcgaga tgagcgtgga gaacgtaaag caggtgctgg
     2281 aatccggaaa cggggtgtgc aatgtgaggc tcgactctcc tgtctgggcg ggagagaaga
     2341 tgacgtttat ggattatatc cccgactcgg tcaccgtgcc cgtggactcg ctgatcgcgg
     2401 aggtctccat tccggaaaag gtcgagagcg cccttcatgt gctcgatgaa agggagaggg
     2461 agatcgtgaa gatgaggttc ggaatcggct ttgagggggc gtttacgctc gacgagatag
     2521 gaaagaggct gggcctgact agggagagga taaggcagat agaaaagaag gccctttcca
     2581 ctttgaggca ttctgactcc gcgcccgcac tgaggagcct gatagagcat tgacgctcaa
     2641 tctgcaggcg ctcgaatgga tataacctgc cttgactctt aattaatacg ttttccgtcg
     2701 tacgtttgga acactcccgg gaataccgct gacactctac caagacctgt tattgaatcc
     2761 atttgcttga caacgg
//
LOCUS       DASSJH010000085         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_993037, whole genome shotgun sequence.
ACCESSION   DASSJH010000085 DASSJH010000000
VERSION     DASSJH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993037"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /gene="eno"
                     /locus_tag="VFJ67_01795"
     CDS             complement(<1..617)
                     /gene="eno"
                     /locus_tag="VFJ67_01795"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248115.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7289142.1"
                     /translation="MSAITSIKAREILDSRGNPTIEVDVYCEDGSFGRAAVPSGASTG
                     EHEAIELRDNDIKRYLGKGVEKAVKNVNRVIAPKLIGMDASSQSALDGLMIGLDGTPN
                     KSKLGANAILGVSLAAARAAANSLGIPLYRYLGGITADTLPVPLMNIVNGGAHADNNL
                     DFQEFMIVPLGFGSFRDAIRAGVEVFHNLKSILKKKGYSTSVGDEG"
     gene            847..2718
                     /locus_tag="VFJ67_01800"
     CDS             847..2718
                     /locus_tag="VFJ67_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289143.1"
                     /translation="MKLAFRLKSFYLFTCISIVALLVSCAVGDSKKPGTISDLTIDEL
                     TRLLEWTAPGDNGNSGTVTIYFPRYYSDQQVAELLGLPNLDGVPFEVIQDTVIDNFKD
                     ATQVPDFQEPLSAGNPESFLSPRLDITGNTSYFYSIRSNDETGSSSNPSNVAELTTPL
                     QDIRYVSGEPGSCIGQSVAAGNYDGEKIVQNVCIIDNDSGSCDGGGLDTTINDIAIGD
                     PCLGMVYIFYGQNDLTDSGNAVIDVSLADVTIIGNAADGFGASLATANSLNSASLAQE
                     LVIGAPDFNNGTGKVYMVFGDKDLPSVIDLVNGSEEHIEVEGENPGDNFGFSLSNGSG
                     VTNGRAVFIVGAPFYNSDTGRAYVFKGGDLDHNNVNQAATSAKAIFTGQEAGGLFGFD
                     IAPLGRIDNNGSDEFGVGAPELGRAYVIFGKSSLQGKDLATDTTDVLVLQGNAADGFG
                     TSISGDGDIDEDGLGTDDVIVGAPGANSDTGSVFLYSGEDLETNLKDGTTPVAETEFT
                     GLNPGDLFGTSISVFPFLTPELISEQRNTAIVLEFKQVNADFGVGTPGVPNGTVYIFF
                     GQDNFPATVSASEADLSVSGQDGDSGFGSLVEELEDVNGDLEDDFAVGGEGFINLFY"
     gene            complement(2747..>2844)
                     /locus_tag="VFJ67_01805"
     CDS             complement(2747..>2844)
                     /locus_tag="VFJ67_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HET7289144.1"
                     /translation="FGFAALIGIFFGIYPARKASKLDPIEALRYE"
BASE COUNT          630 a          787 c          813 g          614 t
ORIGIN      
        1 ccctcgtcgc cgaccgaggt cgaataccct tttttcttaa ggatcgattt cagattatga
       61 aaaacctcga cgcccgcccg gatagcgtcc ctgaaactcc cgaaacccaa gggtactatc
      121 atgaattcct gaaaatcgag gttgttgtcc gcgtgagccc cgccgttaac aatattcatc
      181 aggggcacgg ggagcgtgtc ggccgtgatg cctccaaggt acctgtaaag agggatgccg
      241 agcgaattgg cggccgcgcg cgctgcggcg agggatacgc cgagtatcgc gttcgccccg
      301 agcttcgatt tgttcggcgt gccgtcgagc ccgatcatga gcccgtcgag cgcgctctgc
      361 gaggatgcgt ccatccctat aagcttgggg gctatgaccc tgttcacgtt cttaactgcc
      421 ttctccacac ccttgccgag gtatcttttg atgtcgttgt ccctgagctc gatagcctcg
      481 tgctcgcccg tcgaagcccc cgagggcacg gctgcccttc cgaatgagcc gtcctcgcag
      541 taaacgtcaa cctcgatcgt ggggttgccc ctcgagtcga gtatttctct cgctttgata
      601 gaagttattg cggacacagt ataatcctcc ggattttgcg ggtattggct aagggaaatt
      661 accataggag tcggcggcta tcaaatccgg ggaatttatc ggttattatt aatgcggcct
      721 ccggacccac gccaacacat ctgcaagccg gccccatgtg cggggggttt gcatatccgt
      781 gttggaaata taatacaagt tcggtagaat tttgtgattt aaaataattt tccagggaaa
      841 aaatctatga agctcgcttt cagactgaag tcgttttacc tctttacttg catttcgatt
      901 gtagcgctcc tcgtttcgtg cgcggtcggc gacagcaaga aacccggcac gatatccgac
      961 ctcaccatcg atgagctgac gaggctcctc gaatggacgg ctcccgggga caacggcaac
     1021 agcggcacgg tgacgatata ctttcccaga tactacagcg accagcaggt ggccgagctg
     1081 ctcggcttgc ctaatctcga cggagtccct ttcgaggtta tacaggacac cgtgatagac
     1141 aacttcaagg acgccactca ggtccccgac ttccaggagc ctctctctgc gggcaacccc
     1201 gagagcttcc tctccccgag gctcgatata acgggaaata cgagctactt ctattccata
     1261 cgcagtaacg acgagacggg cagctcctca aacccctcga acgtggccga gcttacgacc
     1321 cctcttcaag atatcaggta cgtgagcggc gagcccggga gctgcatcgg gcagtcggtc
     1381 gcagcgggga attacgacgg cgaaaagatt gtccagaacg tatgtattat cgataacgac
     1441 agcggatcct gcgacggcgg cggattggat acgacaatta acgatatagc cataggcgac
     1501 ccgtgtctcg gaatggtgta catattctac ggccagaatg atctgaccga tagcggcaat
     1561 gctgtgatcg acgtgtctct cgccgacgtt acgataatag gtaacgccgc tgacgggttc
     1621 ggcgcgtctc tcgccacggc aaacagttta aacagtgcca gtttggcgca ggagcttgtg
     1681 atcggtgcgc ccgattttaa taacggcacc gggaaggtct atatggtttt cggcgacaag
     1741 gaccttccgt ctgtcataga cctcgtaaac gggagcgaag agcatattga agtagaaggt
     1801 gagaacccag gggacaactt cgggttctct ctttcgaacg gctcgggcgt tacaaacggc
     1861 agggccgtct ttatcgtggg agctcccttc tacaactcgg acacaggcag ggcctatgtc
     1921 ttcaagggcg gagacctcga tcataacaac gttaatcagg cggcgacgag cgcgaaagcc
     1981 atattcaccg gacaagaggc gggcgggtta ttcggtttcg atattgcgcc cctcgggcgt
     2041 atcgacaata acggctcgga cgagttcggt gtaggcgctc ccgagctcgg cagggcatac
     2101 gtcatattcg gcaagtccag tctccagggc aaggacctcg cgaccgatac gacggacgtc
     2161 ctggtgctcc aggggaacgc cgctgacggg ttcggcacga gcatctcggg cgacggggac
     2221 atagacgagg acgggctggg taccgacgat gtgatagtgg gcgcgccggg tgccaacagc
     2281 gacacgggct cggtcttcct atactcgggc gaggacctcg agaccaatct caaggacggc
     2341 acgacccctg tcgcggagac cgagttcacc ggactcaacc ccggggacct attcggaacg
     2401 tcgattagcg tgttcccatt cctgaccccc gagcttattt cggagcagag gaacactgcc
     2461 atagtgctcg agttcaagca ggtcaacgcg gatttcggcg tcggcactcc gggagtgccg
     2521 aacgggaccg tttacatatt tttcggacag gacaatttcc ccgccacggt ttccgcgagc
     2581 gaggctgatt tatcagtcag cggtcaggac ggcgactccg gtttcggaag ccttgtagag
     2641 gagctcgaag acgtaaacgg cgacctggaa gacgatttcg ccgtgggcgg cgagggattt
     2701 ataaacctct tttattaata gaaaggttcg atcgcgcgcg gtgcctctat tcgtatctga
     2761 gggcctcgat cggatcgagc ttcgacgcct ttcgcgcagg atagataccg aaaaatattc
     2821 ctataagcgc tgcgaacccg aacg
//
LOCUS       DASSJH010000086         8344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_129251, whole genome shotgun sequence.
ACCESSION   DASSJH010000086 DASSJH010000000
VERSION     DASSJH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129251"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..451
                     /locus_tag="VFJ67_01810"
     CDS             167..451
                     /locus_tag="VFJ67_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289145.1"
                     /translation="MSSKTITVEIFQCDHVTDEGERCASEGERMSIKTCALCQKDLCS
                     RHYEQITVSSRGGRDSLTYHFCDEHTQEFMDTLINTFGDTRPVAYAGMAK"
     gene            777..2330
                     /locus_tag="VFJ67_01815"
     CDS             777..2330
                     /locus_tag="VFJ67_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET7289146.1"
                     /translation="MKIATREIVREIDARSIEKHGIPGLVLMENAGRMAADVVLEEFP
                     QVRTAAVFAGGGNNGGDGFVIARHLMGQGVGVKTYLAVSPAKYKGDALANLKALKKSG
                     GEIIELGGSLRKYTEADIIVDALFGTGLDREVEGFNRKIIDFMNGKPVPRLAVDLPSG
                     IDANTGFPLGAAVRADVTVTFVMPKIGISIYPGLGYAGRIFVADITTPKYLEDDVPYE
                     LLTNVSVSGILKPRLPDTHKGTYGHLFILAGSPGKTGAATLASLGALRVGTGLVTVGI
                     PSSLNDIMEVKTTEAMTEPLPETDLRTLGMMSVKRAKEIIANKKTALAMGPGISTTEE
                     TRGFLYEMIRSSEIPAVIDADALTLIAHDPGILLKAKAPLVLTPHPGEMSRLAGIPTD
                     DIERDRIGVSLEFAARHKVILVLKGARSLVTTPSGRVYVNTSGNAGMASGGMGDVLTG
                     IIGGFLAQRIAPEDAARLGVFVHGLAGDLCARERGQAGIIAGDLADVLPRTLAGLPGS
                     DEEPFFRIR"
     gene            2384..2872
                     /gene="tsaE"
                     /locus_tag="VFJ67_01820"
     CDS             2384..2872
                     /gene="tsaE"
                     /locus_tag="VFJ67_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET7289147.1"
                     /translation="MEILVTDAEETIKLGEIIGKTLTPGSIIALRGGLGAGKTVLVKG
                     IARGLGIEDEPVSPTFVIMNAYEGAIPLYHFDLYRILGADELMGIGADEFLFGEGVSA
                     VEWAERVGEILPEYTIYIDIKIPEPDNGSENIREIKIEGKKEWLSSFRSTAEQAFPAS
                     TR"
     gene            2815..4047
                     /locus_tag="VFJ67_01825"
     CDS             2815..4047
                     /locus_tag="VFJ67_01825"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7289148.1"
                     /translation="MALIVQKYGGTSVSSLDKIKWVAGHVINTKEKGNDVLVVVSAMA
                     GETDKLVKMSLQIMDPPDERELDVVTSSGEQVSSGLLAMSIKALGHDALSLQGHQVRI
                     VTDNAHSKAKIVRIDAERIKKALREGKIVVVAGFQGVDEEGNVTTLGRGGSDLTAVAL
                     AAALGADSCELYKDDVDGIYTSDPGMVPDARKLDKISYEEMLELASLGSKVLQARAVE
                     FAMKFGVTLHVRPTSEPDTEGTWVVEEEKMTHLENILISGISSDKNQAKITITQVPDS
                     PGIAAKIFTPLSESGIIVDMIVQNVSIEGHTDMTFTVPRTDFKKSIILTEKVAKELGA
                     RDIISDDKITKVSLVGVGMKTHSGVASRMFSTLAKEGINILMISTSEIKISCVVEEKY
                     TELAVRALHDAFELGEEK"
     gene            4044..5645
                     /gene="cimA"
                     /locus_tag="VFJ67_01830"
     CDS             4044..5645
                     /gene="cimA"
                     /locus_tag="VFJ67_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET7289149.1"
                     /translation="MSRDSVKIYDVTLRDGTQGESISFSIHDKLRIAQKLDELGVHYI
                     EGGWPGSNERDEGFFEEAKRLRLRKSKIAAFGSTRRAGKTCDEDGSVQALLKAETPAV
                     TIVGKSWDFHVKEALRIPLKENIEIIHDSVTYLKKYVDEVFFDAEHFFDGYKHNQKYA
                     IEAIKAAHDAGADVLVLCDTNGGTLPWEIEEIVRDVNLKLDNPHLGIHTHNDGEVGVA
                     NTLYAVREGVKQVQGTMNGYGERCGNANLCSIVPNLKLKLGIDCISEDDLKRLYDVSH
                     FIHELTNLPPLKHRPFVGESAFAHKGGLHVSAVMKKSETYEHVNPDVVGNHRRVLISD
                     LSGRSNILYKAKELGIELDPQDKRVNAILNELKKLENEGYEFEGADASFELLVRKALG
                     EYRKAFELTSVRTITEKRSEDEEPLTEATLIVQVDGVTEHTVANGNGPVNALDLGLRR
                     ALEKFYPALKEVTLKDYRVRVLSRQHGTDAVVRVLIESGDGESDWSTVGVSQNIVEAS
                     WKALVDSIDYKILKDESKRAAKARG"
     gene            complement(5888..6418)
                     /locus_tag="VFJ67_01835"
     CDS             complement(5888..6418)
                     /locus_tag="VFJ67_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET7289150.1"
                     /translation="MFIKRVIRALTLDPELYEEVEHDKSAMWQAVVVVFLSSLSRGVY
                     SYQTGDYKGLILGTTTSFVLWMLLSFLIYLIGTKLFPESDTRSDQWEIMRVLGFASAP
                     GVFRIFAVVHHFTAIVLLVVWVWTLVAMIIGVRQALDFKNTWNAIWVCVVGLITYWLV
                     YMIFYFAYGLPRPVWS"
     gene            complement(6536..7060)
                     /locus_tag="VFJ67_01840"
     CDS             complement(6536..7060)
                     /locus_tag="VFJ67_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET7289151.1"
                     /translation="MFIKRVIRALRLDPEVYEEVEADKGSIWQAILVVVMVSVAAGIG
                     SIAVLGIEGIIVGTLLDLIGWFLWALLTYVIGTKLLPEPQTVSDLGELLRVIGFARAP
                     GIFLILILIPAVGKFVAFVITVWMFVAMVIGVRQALDYKSTWRAAGVCILGAVIYFVI
                     NVAVNSLLVNQGAP"
     gene            complement(7101..7643)
                     /locus_tag="VFJ67_01845"
     CDS             complement(7101..7643)
                     /locus_tag="VFJ67_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET7289152.1"
                     /translation="MAGIIDRMVRASRLDVSVYEEVEADKGAMGQAAAVVVLSSVAAG
                     IGSLGVFGIQGLIYMTFGALVGWLIWAFLTFIIGTKLLPEAQTKTDMGEMLRTIGFSS
                     SPGVLRILGVIPVIGGIINFVVGIWMLVAMIIAVRQALDYKSTWRAVGVCLIGFIVYF
                     VIMLFIFRLIGVNPAIEPTA"
     gene            complement(7724..>8344)
                     /locus_tag="VFJ67_01850"
     CDS             complement(7724..>8344)
                     /locus_tag="VFJ67_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase V"
                     /protein_id="HET7289153.1"
                     /translation="ACMIGELCGENGIALCLDCISSLGTVPIDLSRVYMATGTSGKGL
                     RSYPGLSFVFHNHEIESRPDKLPRYIDLGLYAESAGTPFTLSSNLLFALGASLDNLRA
                     DERYARIRSLSGWLRERLSDAGFEIVAERGHEAPAIITIPLPERLGSRALGMRLERSG
                     YSLNWRSGYLLERNWIQIALMGDCTEAGLDALAGLLSEEVKSRRRA"
BASE COUNT         2106 a         2319 c         2370 g         1549 t
ORIGIN      
        1 ccagacgggg tacggcgtca cccttcccag gctcaggcat tgacgggaag acggcgtata
       61 ataatccata ttgacatggg cgccgggatt tagttaaatg agatggtgga gagggcggct
      121 caaaggcgtc cgggaatatt cggtattgat tacaaggaga ttttaaatgt ccagcaaaac
      181 aattacagtc gaaatattcc agtgcgacca cgtgaccgac gaaggcgagc gctgcgccag
      241 tgaaggcgag aggatgtcga taaagacctg cgccctttgc cagaaggacc tctgcagcag
      301 gcactacgaa cagataaccg tctccagccg gggcgggagg gattcgctca cataccattt
      361 ctgtgacgag catacgcagg agttcatgga cacgctcatc aacaccttcg gcgacacgag
      421 gcccgtcgcc tatgccggca tggcgaagta gttgttgtac ccggcttttt ctgcctccac
      481 ggccttcctg tcgatcctca attcgtagga gcggcttcca gccgcgatac aatttcaaat
      541 cccccttaat ccccctttac agagggggaa aatgacattc cccaattcta ggtctatcat
      601 ctcatctttt ttcatataat tcaccgctta ggaaaaggaa ggtcaaggag gatttagcta
      661 cttgcagtta tctcaccccc gtccgagaaa tctctcctaa attaaacctt tactttaaaa
      721 ctatcactct acacctatac aatcccaccg atttacgtta ttttttatgc acgaacatga
      781 aaatagcgac acgcgagata gtaagggaaa tcgacgccag atcgatagag aagcacggca
      841 tccccggcct cgtcctgatg gagaacgccg ggcgcatggc ggcggacgtc gtcctggaag
      901 agttccccca agtgaggaca gcggccgtat tcgcgggcgg cgggaacaac gggggcgacg
      961 gattcgtcat cgcccgccac ctcatgggcc agggggtagg cgtcaagacc tatctcgccg
     1021 tgagccccgc gaaatataaa ggcgacgcgc ttgctaattt gaaagcgcta aagaaatcgg
     1081 gcggagagat catagagctc ggcggcagtc tcaggaaata cacggaggca gacatcatag
     1141 tggacgcgct cttcggcacg ggcctcgata gggaagtcga agggttcaac aggaagataa
     1201 tagatttcat gaacggaaag ccagtcccgc gcctcgccgt cgatctccct tccggtatag
     1261 acgccaacac cgggttccct cttggtgctg ccgtccgggc ggacgtaact gtaacgttcg
     1321 tcatgccgaa aataggcata tccatatacc cggggctcgg ctatgcgggc aggatattcg
     1381 tcgccgacat cacaacgccg aaatacttgg aagacgacgt gccatacgag ctgctgacga
     1441 acgtgtccgt gagcgggata ctgaaacccc gcctccccga cacgcacaag ggcacgtacg
     1501 gtcacctttt catactcgcg ggctctcccg gaaagaccgg ggccgcgacg ctcgcgtctt
     1561 tgggagcgct cagggtgggc acggggctcg ttacggtggg aattccgtcg agcctgaacg
     1621 acataatgga agtgaagacg acagaggcga tgacggagcc cctccccgaa acggacctcc
     1681 gcactttggg aatgatgtcc gtaaagcgcg cgaaggaaat aatcgcgaat aaaaaaacgg
     1741 cgctcgctat ggggcccggc atatccacga ccgaggagac ccgcgggttc ctctacgaga
     1801 tgataaggtc gtcggagatt ccggcggtga tcgacgccga cgcgcttacc cttatagccc
     1861 acgatccggg aatactgctc aaggcgaagg cccctctcgt gctcactccc catcccggcg
     1921 agatgtcccg tctcgcggga atcccgaccg acgacataga gcgggacagg atcggcgtgt
     1981 ctctcgaatt cgcggcgaga cataaagtaa tactcgtgct caagggagca aggtctctcg
     2041 tcacgacgcc aagcggccgc gtttacgtga acacctcggg caacgccgga atggcctcgg
     2101 gcggcatggg cgacgttctc accggcataa taggcgggtt cctcgcgcag cgtatcgcac
     2161 ctgaagacgc ggcgaggctc ggggtattcg tgcacggcct tgccggcgac ttgtgcgccc
     2221 gcgagcgcgg acaggcgggc ataatagcgg gcgacctcgc agacgtcctc ccacggacgc
     2281 tcgccgggct ccccggttcg gacgaggaac ctttcttccg aattcgctga tatcactcac
     2341 tttttaatct caaagaaaaa ccgatatgac ggaaagagag agtatggaaa tactggtgac
     2401 cgacgctgaa gaaacaataa aactgggcga gattattggc aaaacgctca cgcccggtag
     2461 tattattgcc ctcagggggg gcctgggagc gggcaaaacg gtcctcgtca agggcatcgc
     2521 cagggggctc ggaattgaag acgagcccgt gagcccgacg ttcgtcataa tgaacgcgta
     2581 cgagggcgca attccgctct accacttcga cctctacagg atcttgggcg cggacgagct
     2641 tatgggaata ggagccgacg agttcctctt cggagaaggg gtatcggcgg tcgagtgggc
     2701 ggagcgggtc ggtgaaatat tgcccgaata tactatatat atcgacataa agattccgga
     2761 accggacaac ggttcggaga acataagaga aataaaaata gaagggaaaa aagaatggct
     2821 ctcatcgttc agaagtacgg cggaacaagc gtttccagcc tcgacaagat aaagtgggtg
     2881 gcgggtcacg taatcaatac gaaggagaag ggtaacgacg tcctcgtggt cgtatcggcg
     2941 atggcgggcg agacggacaa gctcgtaaag atgtcgctcc agataatgga ccctcccgac
     3001 gagagggagc tcgacgtggt cacttcgagc ggcgagcagg tttcctcggg cttgctcgcg
     3061 atgtcgataa aggcgctcgg gcacgacgct ctctcgctcc agggacatca ggtgaggatc
     3121 gtcacggaca acgcccactc gaaagcaaag atagtcagga tagacgcgga gaggataaag
     3181 aaggcgctcc gggaaggaaa gatcgtcgtc gttgccgggt tccagggcgt ggacgaggaa
     3241 gggaacgtca cgacgctggg caggggaggc tcggatctca ctgccgtcgc gctcgcagcg
     3301 gcgttaggcg ccgactcgtg cgagctgtat aaagacgacg tggacggcat atacacttcc
     3361 gaccccggca tggtgcccga cgcgcggaag ctggacaaga tttcttatga agagatgctc
     3421 gagctcgcga gcctcggttc gaaggtgctc caggcgcggg cggttgagtt cgcgatgaag
     3481 ttcggcgtca ctctccacgt ccgccccaca tcggagccgg acacggaagg aacatgggta
     3541 gtagaggagg aaaaaatgac tcatctggaa aatattctca tatcgggcat atcgtccgac
     3601 aagaaccagg ccaagataac gatcacgcag gtgcccgaca gccccggcat cgcggccaag
     3661 atattcaccc ctctcagcga gagcgggata atcgtggaca tgatagtcca gaacgtgagc
     3721 atcgagggcc acaccgacat gacgttcacg gtgccgagga cagattttaa aaaatcgatt
     3781 atactaactg agaaggtggc gaaggagctc ggggccaggg acatcatctc cgacgacaag
     3841 attacgaagg tttcgctcgt cggggtcggc atgaagaccc actccggggt cgcgagccgt
     3901 atgttttcga ccctcgctaa agagggaata aatatattga tgataagcac gtccgaaata
     3961 aagatttcct gcgtcgtcga ggagaaatac acggagctcg cggtaagggc gctccacgac
     4021 gcgttcgagc tgggagaaga gaaatgagcc gggattcggt aaagatatac gatgtaactc
     4081 tccgcgacgg gactcagggg gagagcatat cgttctccat acacgacaag ctgaggattg
     4141 cccagaagtt agacgaactc ggcgtacact acatcgaagg cggatggccg ggctcgaacg
     4201 aaagggacga ggggttcttc gaggaagcga agaggctcag gttaaggaaa tcgaagatag
     4261 ccgcgttcgg aagcacgaga cgcgccggga agacctgcga tgaggacggg agcgtacagg
     4321 cgctgcttaa agccgagacc cctgccgtaa ccatcgtcgg caagagctgg gacttccacg
     4381 taaaggaagc gctcaggata ccgctcaagg aaaacatcga aataatccac gactcggtca
     4441 cgtatctcaa gaaatacgtg gacgaggtat tcttcgacgc cgagcacttc ttcgacggct
     4501 acaagcacaa ccagaagtac gcgatcgaag cgataaaggc cgctcacgac gcgggagccg
     4561 acgtgctcgt actctgcgac acgaacggcg gcacgctccc ctgggagata gaggagatag
     4621 tcagagacgt taacctcaag ctcgacaacc cgcacctcgg catacacact cacaacgacg
     4681 gcgaagtggg cgtcgcgaac acgctctacg ccgtcaggga aggggtaaag caggtgcagg
     4741 ggacgatgaa cggctacggg gagaggtgcg ggaacgcgaa cctctgctcc atcgtgccta
     4801 acctcaaatt gaagcttggc atagactgca taagcgaaga cgacctcaag agactctacg
     4861 acgtatcgca tttcatacac gagctcacca acctgccgcc cctcaagcac aggcctttcg
     4921 tcggcgagag cgccttcgcc cacaagggcg ggcttcacgt gagcgcggtg atgaagaaat
     4981 cggagacata cgagcacgta aacccggacg tcgtcggcaa ccacaggcgc gtcctcatat
     5041 cggacctctc cgggcggagc aacatcctct acaaggcgaa agagctaggc atagagctcg
     5101 acccgcagga caagcgcgtg aacgccatac ttaacgagct caagaagctc gagaacgaag
     5161 ggtacgagtt cgagggcgcg gacgcgtcgt tcgagctgct tgtaagaaag gccctcgggg
     5221 aatacaggaa ggcgtttgag ctaacgagcg ttcgcaccat caccgaaaag agaagcgagg
     5281 acgaagagcc tctcacggag gcgacgctca tagtccaggt ggacggcgtc acggagcaca
     5341 cggtcgcgaa cggcaacggg cctgtcaacg cgcttgacct tggcttaagg agggcgctcg
     5401 agaagttcta ccctgcactc aaggaagtca cgctgaaaga ctacagggta agggtgcttt
     5461 cgagacagca cggcacggat gcggtagtgc gagtgctcat agaatcgggc gacggcgaga
     5521 gcgactggag cactgtagga gtctcacaga acatagtcga ggcgagctgg aaggcgcttg
     5581 tcgacagcat cgattacaag atcctgaaag acgaatccaa gcgcgcagcc aaagcgcggg
     5641 gatgagtcaa aagaaaaatc ctccctaacc tatttcgact ttagcgacgt tcagccggaa
     5701 gttaatttac tttcatgcct ttgttatcag ggcgtccgaa tgcgcgccag cttgcattcg
     5761 ttctttttct aaggaggtaa tttaaaaaac aagataaaga caagaccctg aaacaagttc
     5821 agggtgacga tataaggttt gtccgaggga aaaagcagta accgtacgtc agcacacatt
     5881 gtgtgcatca cgaccatacg ggcctcggca gtccgtatgc gaagtagaat atcatgtaca
     5941 caagccagta cgtgatgagt cccaccacgc acacccagat ggcgttccag gtgttcttga
     6001 aatcgagcgc ctgcctcacg ccgattatca tcgcgacgag cgtccacacc catacgacca
     6061 gcagcacgat cgccgtgaag tgatgcacga ccgcgaagat gcgaaatacg ccgggcgcgc
     6121 tcgcgaatcc gagcacgcgc attatctccc actggtcgct cctagtatcc gattccggga
     6181 agagcttcgt gccgattaag taaattagga aagaaagcag catccagagc acgaagctcg
     6241 tcgtcgtgcc gaggataagc cctttgtagt cgcccgtctg gtagctgtac acgcctctcg
     6301 agaggctcga gaggaagacc acgaccacag cctgccacat cgcgctcttg tcgtgctcga
     6361 cctcttcgta aagctccggg tcgagcgtga gcgcccttat cactctttta ataaacacca
     6421 atgcctttca cctcagcact aataattatt gggattagaa gataaatcta acgattccat
     6481 aagtcaaaat acgcagggag tcaggcggca aacttctatc gtacgtccgc gcctcctagg
     6541 gggcgccctg atttaccagg agcgaattca ccgcgacgtt aatgacgaaa taaatgacgg
     6601 cgcctaatat gcacacgccc gcggcgcgcc acgtgctctt gtagtcgagc gcctgcctca
     6661 cgccgatcac catcgccacg aacatccaga cagttataac gaacgcgacg aacttgccta
     6721 cagccggtat aagtatgagg ataagaaaaa ttccgggcgc cctggcgaac cctattaccc
     6781 tgagcaattc cccgagatcg gataccgtct gaggctcggg cagaagcttc gtgccaatta
     6841 cataagtcag taaagcccat agaaaccatc cgataaggtc gagcagcgtc ccgacgatta
     6901 tcccctctat ccccaaaacc gcaatcgagc cgatacccgc cgcgacgctt accatcacga
     6961 ccacaagtat cgcctgccat atagacccct tatcggcctc gacctcttcg tacacctccg
     7021 ggtcgagcct gagcgccctt atgactcttt taataaacac ggatgccgtt cacctctgct
     7081 atggtagtac ggttaacaat tcaggcagtc ggctcgatcg cagggtttac accgatcagc
     7141 ctgaagatga aaagcatgat gacgaagtac acgataaatc cgattaaaca cacgcccacg
     7201 gcgcgccaag tgcttttgta gtcgagcgcc tgtctcaccg ctattatcat agcgacgagc
     7261 atccagatgc cgactacgaa atttatgatc ccgccgatga cagggataac gccgaggatg
     7321 cggagcaccc cgggtgagct cgaaaagcct atcgtcctga gcatctcgcc catgtcggtt
     7381 ttcgtctgcg cctcgggcag cagcttggtg cctatgataa aagtcagaaa cgcccatatc
     7441 agccacccga ccagagctcc gaaggtcatg tatatgagtc cctgtattcc gaacacgccc
     7501 agcgagccta tacccgcagc gacgctcgag agcactacca ccgcagccgc ctgtcccatt
     7561 gcacccttgt cagcttcgac ttcctcatag acactcacgt cgagcctgga agccctgacc
     7621 atcctatcga ttattcccgc catcttcgtt cctccgcagt gtacttacaa atatgcacat
     7681 aataaaactt cccgagcgat tcgtcaaaaa gcggatgaaa gtcctatgcg cggcgtctgg
     7741 acttcacttc ttccgaaagg agaccggcga gggcatcgag tccggcctcg gtgcagtccc
     7801 ccatgagcgc gatctgtatc cagttccgct cgagcagata gccgctcctc cagttgaggc
     7861 tgtaacccga ccgttcgagt ctcatcccga gggcccggga gccgagcctt tcggggagcg
     7921 ggatggttat aatggcgggc gcttcgtgtc ccctctcggc gactatttcg aacccggcgt
     7981 cagaaagtct ctccctcagc cagccggaga gtgagcgtat ccgggcgtat ctctcatcgg
     8041 cacggagatt gtcgagcgac gctccgagcg caaaaagcag gttcgatgac agcgtgaacg
     8101 gcgtgcccgc cgactccgcg tacaagccga ggtcgatgta acgggggagc ttgtccggcc
     8161 gggattcgat ctcgtgattg tggaatacga acgagagccc gggataggac ctgagcccct
     8221 tcccgctcgt tcccgtcgcc atgtaaacgc gcgagaggtc tataggcact gttccgagcg
     8281 agcttataca gtcgaggcag agcgcaattc cattttcgcc gcagagctct ccgatcatac
     8341 aggc
//
LOCUS       DASSJH010000087         4386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_884732, whole genome shotgun sequence.
ACCESSION   DASSJH010000087 DASSJH010000000
VERSION     DASSJH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884732"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1355)
                     /locus_tag="VFJ67_01855"
     CDS             complement(138..1355)
                     /locus_tag="VFJ67_01855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7289154.1"
                     /translation="MKTSVIDYRQGDPPDDTPLFNRDFVLVTLTSFIFFFTYHSFILL
                     PLRIEELGGSESVIGFIMGSASAATILTTPSVGILIDKWGKKWFLLAGGLLMSVTSLP
                     FAFLDSLGPAFPALRILHGAALSLCFVSAGTLIADVSSPARRSQAIGIFGLFSVINFA
                     LAPFVGKKIVEAYGFGDLFIFDFFFGSSAFVAALFIREPAAFSPDGVKGSGFREALFR
                     SGVFPAAFALLVAGTGFVSAITFVPVFAKRIGVHSFELFFITYTVAIIAVRLIGGWIP
                     DKFGKKRASIPAFFMFAAGIVAIGLASGPMGLVLAGVFFGLGHGLFYPAIYALVIDLS
                     PEADRGKAVSICSVAFTLGGMLGVFIYGVVAEHWGFRLMFDVSGAVCAIGFILFAFKG
                     KEAESGFSRLSER"
     gene            1478..1978
                     /locus_tag="VFJ67_01860"
     CDS             1478..1978
                     /locus_tag="VFJ67_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289155.1"
                     /translation="MVRNTLIVILAALLSVTSYLLYTNRSISINVGGSAAECDTGAPV
                     ESKVENASPGSVDQETPASAENANPPREASSEDVEKVESEEPEVHLDPTVGMSINYEQ
                     QIATSYKIIGPRKFSLLTDEDETMVEINLDTGNVKINPKYKVDDVTKEFWKSVGRKYP
                     EVCFVE"
     gene            complement(1997..3280)
                     /locus_tag="VFJ67_01865"
     CDS             complement(1997..3280)
                     /locus_tag="VFJ67_01865"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7289156.1"
                     /translation="MSILIKGGRIIDPSRNFDKVGNILIEKGAVKSYPDDIKKLEKHS
                     DVKVIDAAGKIVCPGLVDLHVHLREPGYEHKETIRTGCESAAAGGFTSIVCMPNTNPI
                     NDNASVTEYIMLKARTEGIVNVFPLGAITKGENGETLAQIGEMYEAGCVGVTDDGMPV
                     MNSKVMRHAMEYVKAFDIPVISHSEDLNLSGNGVMNEGDMSTRLGLTGIPAASEDVMV
                     SRDITLAELTGTRLHICHVSTAGSVRLIRAAKGRGVKVTAEAAPHHFTLTEKAVAEYD
                     TNAKMKPPLRSEADREAVREGLRDGTIDVIATDHAPHSEDEKMVEFDLAPFGIVGLET
                     ALPLSLKLVEDGVLTLNEMIAKLTHLPSAVIKVNKGTLNPGDAADILIFDPDKKVKID
                     RQRFRSKSKNTPFSGWDLKGAVLYTIVNGNIVYSD"
     gene            complement(3277..4206)
                     /locus_tag="VFJ67_01870"
     CDS             complement(3277..4206)
                     /locus_tag="VFJ67_01870"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET7289157.1"
                     /translation="MAFERKHILGLEELTPEEISLILDTAESMKEVSERDVKKVPALR
                     GVTVCNLFFEPSTRTRSSFEIAEKRLSADVLNFTTSQSSVVKGESLLDTARNLEAMGA
                     SMIVIRHPMSGAPWLLAKKLESSIINAGDGSHEHPSQALLDLFTIRQIKGRIEGLKVV
                     IVGDILHSRVARSNIWGFMKLGAEVRVVGPPTLVPKYIESIGVKSYYSLDKAVEGADV
                     VIMLRIQKERQDSEYFPSLREYSRFYGLTREKLRCADKDVTVMHPGPINRGVELSSDI
                     ADGPGSVILEQVANGIAVRMAMFYLVASARSSS"
     gene            complement(4210..>4386)
                     /locus_tag="VFJ67_01875"
     CDS             complement(4210..>4386)
                     /locus_tag="VFJ67_01875"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619335.1"
                     /note="regulates pyrimidine biosynthesis by binding to the
                     mRNA of the pyr genes, also has been shown to have uracil
                     phosphoribosyltransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase"
                     /protein_id="HET7289158.1"
                     /translation="DYGRPSSIQLAVLVDRGHRELPIQADYTGIKLSTLSTEEVRVHL
                     KEADGRDEVIIVTE"
BASE COUNT          965 a         1283 c         1187 g          951 t
ORIGIN      
        1 ccgggacgca tttccactat ctctatgccc aggaactccc agaacggtac aacggggaaa
       61 tcatttatcg aaattttctc ttcagtcatc tgccgcctcc gtcgttatct cgctatatct
      121 tacccgacac tccgatttta gcgctcactc agacgggaga acccgctctc agcctccttt
      181 cccttaaaag cgaagagaat gaaaccgatc gcgcacacgg cgcccgacac gtcgaacatc
      241 aggcggaaac cccagtgctc ggcgaccacg ccgtagatga acacgccgag catcccgccc
      301 agagtgaagg ccacgctgca tatggagaca gccttccccc tgtcggcctc gggcgagagg
      361 tctatgacga gggcgtatat ggcggggtag aaaagcccgt ggcccagccc gaagaatacg
      421 cctgccagca caaggcccat cgggcccgag gcgagcccta tggcgactat acccgcggcg
      481 aacatgaaaa aggccggtat ggacgccctc ttctttccga acttgtcggg tatccatccg
      541 cctatgagcc ttaccgcaat aatcgcgacc gtgtacgtga taaaaaagag ctcgaacgag
      601 tgaacgccta tgcgcttcgc gaatacggga acgaacgtaa tggcggaaac gaaacccgtg
      661 cccgccacca ggagcgcgaa agcggccggg aacacgccgc tcctgaaaag cgcctccctg
      721 aatccgcttc cctttacgcc gtccggagag aatgcagccg gctccctgat gaagagggca
      781 gccacgaagg ccgatgagcc gaaaaagaaa tcgaatatga agaggtcccc gaaaccgtag
      841 gcttccacga tcttcttccc cacgaaaggc gcgagcgcga agttaatcac gctgaagaga
      901 ccgaagatcc cgatcgcctg gctcctcctc gccggtgaag agacgtccgc tatcagcgtg
      961 cccgcggata cgaagcagag ggagagcgcc gccccgtgca gaattctcaa ggcggggaac
     1021 gcggggccga gagagtcgag gaaagcgaag gggagcgagg ttaccgacat gaggagacct
     1081 cccgcaagca ggaaccactt cttgccccac ttgtctataa ggatcccgac cgaaggcgtg
     1141 gtcaggatag tcgccgcgga agccgatccc attataaacc ctataacgga ctcggagccg
     1201 ccgagctctt ctatacggag cggcaacaga ataaaagagt ggtacgtgaa gaagaatatg
     1261 aacgacgtga gcgtgaccag aacgaaatcg cggttaaaga gcggcgtgtc gtccggaggg
     1321 tctccctgcc tgtagtcgat aacggatgtt ttcatgagaa tccgcggccg tgattatctt
     1381 ataccacaaa aataagaaga gaaatcagtg atcggcaggt tatacttctt aagggagcat
     1441 ggagcggact ttataagaaa gtcccggggg aacgaatatg gtgagaaata cccttattgt
     1501 aatccttgca gcgctcctct cagtcacctc ttacctgctt tatacgaaca ggagcatatc
     1561 cataaacgtg ggcggctctg cggccgagtg tgataccggc gcgcccgttg aaagtaaagt
     1621 agagaacgct tctcccggat cggtcgatca ggaaacgccg gcgtccgcgg aaaacgcgaa
     1681 cccgccccgt gaggcttcgt ccgaggacgt ggagaaggtc gagtcggagg agcccgaggt
     1741 acacctcgac ccgaccgtgg ggatgagcat taattacgag cagcagatag cgaccagcta
     1801 caagatcata gggcccagga aattttctct ccttaccgat gaggacgaaa cgatggtgga
     1861 aattaacctc gacacgggga acgtcaagat aaaccccaag tacaaggttg acgacgttac
     1921 gaaagagttc tggaaatctg tcggaaggaa atatcccgag gtctgtttcg tggaatagcc
     1981 cggcgcggct cccggttcaa tccgaataaa ctatattgcc gtttactata gtgtagagga
     2041 cggccccttt gagatcccac ccgctgaagg gggtgttttt gctcttcgag cggaacctct
     2101 gcctgtcgat cttaaccttt ttatcggggt cgaatatcag tatgtcggcc gcgtcgcccg
     2161 ggttgagcgt ccctttattc accttaatca cagccgaagg caggtgagtg agcttggcga
     2221 tcatctcgtt cagcgtgagg actccgtcct cgacgagctt tagcgaaaga gggagagccg
     2281 tctcgagccc gacgatgccg aagggggcga gatcgaactc caccattttt tcgtcctcgc
     2341 tgtgaggggc gtggtcagtc gctataacgt ctatcgtgcc gtccctgaga ccttccctca
     2401 cggcctccct gtcggcctcg ctcctcaaag gcggtttcat ctttgcgttc gtatcgtatt
     2461 ccgcaaccgc cttctccgtg agggtgaaat gatgaggcgc ggcctcagcc gtcactttca
     2521 ctcccctccc tttggctgcc cggataagcc tgacagaccc ggccgtggat acgtgacaga
     2581 tgtggaggcg ggtccccgta agctctgcga gagtaatatc acgtgacacc attacgtcct
     2641 cggacgcggc ggggatgccg gtcagcccga ggcgagtcga catatcccct tcgttcatca
     2701 caccgtttcc cgagaggttc aggtcctcgg aatgagatat caccggtata tcgaacgcct
     2761 tgacgtactc catggcatga cgcatcactt tcgagttcat cacgggcatg ccgtcgtcgg
     2821 ttaccccgac gcagccggct tcatacatct ctcctatctg cgcgagggtc tcgccgttct
     2881 cgcctttcgt tatggcgccg agcgggaata cgttcactat gccttcagtg cgcgccttga
     2941 gcattatata ttccgttacg gaagcgttgt cgttaatggg gttcgtgtta ggcatgcaga
     3001 cgatcgaagt gaagccgccc gccgcagcgg actcgcaccc cgtgcgtatc gtctccttgt
     3061 gctcgtatcc gggctctctc agatggacgt ggagatcgac gagaccggga cagactatct
     3121 tgcccgcggc gtcgatcact ttgacatccg aatgcttttc gagcttcttg atgtcgtccg
     3181 ggtacgattt tacagcccct ttttctataa ggatgtttcc gaccttgtcg aagttcctgg
     3241 aagggtctat tatccttccc cccttgataa gtatgctcat gagctgctcc tcgcggaggc
     3301 gaccagataa aacatcgcca tcctcacggc tatgccgttt gcgacctgct cgagaatcac
     3361 cgagccggga ccgtccgcta tatccgaaga aagctcgacc cccctgttga ttggtccggg
     3421 atgcatcacc gtaacgtcct tatcggcgca cctcagtttc tcccgggtga gcccgtagaa
     3481 cctcgaatac tccctgagcg aagggaaata ctcggaatcc tgcctttcct tctgtatcct
     3541 gagcataatc acgacatcag cgccttcaac ggccttatcg aggctgtaat acgatttcac
     3601 accgatgctc tctatatatt tcggcacgag cgtcggaggc cctaccaccc ttacctcggc
     3661 tccgagcttc atgaatcccc agatattgga cctagccacg cggctgtgga gaatgtcccc
     3721 cacgatcacc accttcagtc cctcgatcct tcccttgatc tgcctgatcg tgaacaggtc
     3781 gaggagcgcc tggctcgggt gttcgtgaga accgtcgccc gcgttgatta tagaggattc
     3841 gagcttcttc gcgaggagcc acggcgcgcc cgacatgggg tgccttatga ctatcatgct
     3901 cgcgcccatc gcctcgaggt tcctcgccgt atcgaggagg ctctcgccct tgacgacgct
     3961 gctctgtgac gtggtgaagt taagaacgtc ggcgctcagc ctcttctcgg caatctcgaa
     4021 agacgaccgc gtacgcgtgc tcggctcgaa gaagaggttg catacggtga cgcctctcag
     4081 ggcggggacc ttcttcacgt ccctttccga gacctctttc atggactccg ccgtatcgag
     4141 tatgagggat atctcctcgg gcgtcagctc ttcgagtccc agtatgtgtt ttcgctcaaa
     4201 agccatcttt tattccgtga ctattattac ttcgtccctg ccgtccgctt ccttcaaatg
     4261 caccctgact tcctcggtgc tgagagtgct gagcttgatg cctgtatagt cagcctgtat
     4321 cgggagctcc ctgtgtcctc tgtccaccag tactgcgagc tgtatggaag aaggtctgcc
     4381 gtaatc
//
LOCUS       DASSJH010000088         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1210443, whole genome shotgun sequence.
ACCESSION   DASSJH010000088 DASSJH010000000
VERSION     DASSJH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210443"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFJ67_01880"
     CDS             <1..849
                     /locus_tag="VFJ67_01880"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7289159.1"
                     /translation="ILQLVGEGLLDLNEPVGKYLPDVENSDATIAELANMKSGIFNYT
                     EDAEFVTEFIESNFLRVWTDQEIVDFADSNVPYFPPGGGWHYSNTNTVILGMIVEQVT
                     GHPLGEEIRKRIIEPLGLDGTSYPTTPDMPEPFAHGYGFDPLEDISFTDPSSSSGSGA
                     MISRLDDLRKWGKALGKGTLLSAELQAERIDSLVPIVFDPCDDDDPDRPKRSCPEYDK
                     YGMGIGEISGWIGHTGEYVGYTSLVMYHPESGSVVVIVTNIFGVASHVPTKIFKEFAE
                     ILNQQL"
     gene            complement(894..2129)
                     /locus_tag="VFJ67_01885"
     CDS             complement(894..2129)
                     /locus_tag="VFJ67_01885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7289160.1"
                     /translation="MIKMPLARLLITVIIISVPALGSGKAAEAEIQAEENAVFTPVMM
                     SALNAPDPVKVTDGRYHLVYEIEAVNASGLTWEISSIDVTDGNGKVLHSLKAAELKNR
                     TELIKDKSKTNALPPGETAVIFSHFTVEDESNIPAYIGHRISITVPGGIPAGFLKFAG
                     LPETDKTYDYTLDVVKTGPSDAIVIGPPLEGEGWVAADGCCDAVRHIRAIMPINDRLR
                     AAQRFAIDWERMNGERKIYSGDPRDVTSYFAYGQRALAVADAKVVAAVDKYKDQVPGA
                     LPPGMTLEEADGNHVVLDLGGGKYALYAHFKPGSVKVKAGDTVKRGQVLGIVGNTGNS
                     SAPHLHFHVMDGLSTFGSNGLPYVIDSFELIEKIPSTEAFDKAEADGTPAEVVPVDKP
                     GTHTNELPLDQSVVDFRTD"
     gene            2294..>2760
                     /locus_tag="VFJ67_01890"
     CDS             2294..>2760
                     /locus_tag="VFJ67_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HET7289161.1"
                     /translation="MNGTSVMESVRQNWGWLLALGIAYIIMGLVITGSPMAATLAVDI
                     LIGFVLIIGGVISIIGAFFSGNWKRLLLILLSGILYLVVGVLLLKNPMQGVLTLTILL
                     AAFLLVEGFFKIIHAFQMKPAPSWVWLLVSGVASVILGIMIWGEFPASSAFIIG"
BASE COUNT          601 a          728 c          764 g          667 t
ORIGIN      
        1 atactgcagc tcgtcggaga gggactcctc gatctgaacg aacccgtcgg gaaatacctc
       61 cctgacgtcg agaattccga cgctactata gccgagctcg cgaacatgaa aagcggcatc
      121 ttcaattaca cggaggatgc ggaattcgtg acggaattca tagaaagcaa ttttttacgt
      181 gtctggacgg atcaggagat tgtcgacttc gccgacagca atgttcccta ctttccgccc
      241 ggtggaggat ggcactattc caacaccaac acggtgatac tcggtatgat agtcgagcag
      301 gtgacgggac acccgctcgg tgaagagata aggaaaagaa taatcgagcc tctcggtctc
      361 gacggcactt cataccctac gacacccgat atgccggagc cttttgcaca cggttacggt
      421 ttcgaccctt tagaggatat aagcttcacg gatccttcgt cgtcctccgg ctcgggcgcc
      481 atgatatcga ggctcgatga tctcaggaaa tgggggaagg ccctcggcaa gggaacgctc
      541 ctcagcgcgg agctgcaggc cgagcggata gacagcctag tgccgatagt attcgatccc
      601 tgtgatgacg acgatccgga caggccgaaa aggagctgcc cggagtacga caaatacgga
      661 atgggtattg gcgaaatcag cggatggatt gggcatacgg gagaatacgt cggctatacg
      721 tccttagtga tgtaccatcc ggaaagcggg tccgtcgtcg tcattgtgac gaacatattc
      781 ggcgtagcgt cgcacgtgcc taccaagatt ttcaaggagt tcgccgaaat acttaatcag
      841 cagctttaga gcagaattga ttcccgcacc cggatcgggg gtgtcccaag tcgttaatcc
      901 gtgcggaaat cgaccacgct ctggtcaagc ggaagctcgt tcgtgtgcgt tcccggtttg
      961 tccactggta ctacttcggc gggtgtgccg tcggcctccg ctttatcgaa agcctcggtc
     1021 gaggggattt tttcgatgag ctcgaagctg tcaataacgt agggcagtcc gttcgagccg
     1081 aaggtggaca gcccgtccat aacgtgaaag tggaggtgcg gggcggacga gttgcccgtg
     1141 ttcccgacta ttcccaggac ctgtccgcgc tttacggtat ctcccgcttt gaccttaacg
     1201 ctcccgggct tgaaatgcgc gtataaggcg tatttcccgc cgccgaggtc gagcacgacg
     1261 tggttgccgt ccgcctcttc gagggtcatt ccgggcggaa gcgcccccgg cacctggtct
     1321 ttgtatttgt ccacggcagc tacgactttg gcgtcagcca cggccagagc tctctgaccg
     1381 tatgcgaaat agctcgtaac gtccctgggg tctccggaat aaatttttct ctccccgttc
     1441 attctttccc agtctatggc gaagcgctgc gcggcccgga gcctgtcgtt tatcggcatt
     1501 atcgcccgta tgtgccttac ggcgtcgcag cacccgtcag cagccaccca gccttcccct
     1561 tcgagaggcg gcccgatgac tatcgcgtcg gacggaccgg ttttaaccac gtcaagcgtg
     1621 taatcgtacg tcttatctgt ctcgggcagg ccggcgaatt tcagaaatcc cgcgggtatg
     1681 ccgcccggta ccgttatgga tattctatga ccgatatagg cagggatatt actttcgtcc
     1741 tcgaccgtga aatgactaaa aataacggcc gtttctcccg gcggcagggc attcgtcttc
     1801 gatttatcct tgatgagctc tgtcctgttt ttgagctccg ctgctttcaa cgaatgaagg
     1861 accttcccgt ttccatccgt cacgtcgata gagcttatct cccacgtgag gccggaggcg
     1921 ttcacggctt caatctcgta tacgaggtgg tatctgccgt cagtgacctt gacggggtcg
     1981 ggggcgttga gggccgacat catcacagga gtgaatacgg cattttcctc ggcttggatt
     2041 tcggcttccg ccgctttgcc cgagccgaga gccggaacgc ttattataat caccgttatc
     2101 agaagacgtg caaggggcat cttgatcata tgatatcgac ctccggaaag cttgatcggt
     2161 ttttacattg tatcatcgcg gctgcattaa taacaggcgt gcggaattaa taagagcgga
     2221 ttttatttat ctcgaaatga acatataatt tgtagaacat cgaaacaaat tcgagcatat
     2281 ggaggaatga gacatgaacg gtacatctgt aatggagagt gtaaggcaaa actggggatg
     2341 gcttcttgcc cttggtatcg catatataat tatgggcctt gtcataacgg ggtccccgat
     2401 ggcggcgacg ctggcagtcg atatactcat cgggttcgta ttaataatcg gaggagtgat
     2461 ctccataatc ggcgcgtttt tctcggggaa ctggaaaaga ctgctgctga tattgttaag
     2521 cggcatactc taccttgtcg taggagtgct tctgctgaag aacccgatgc agggagtgct
     2581 cacgctcacc atactcctcg ccgcgttttt actcgtggaa ggttttttca agatcataca
     2641 cgcgttccag atgaagcccg cacccagctg ggtatggctc ctcgttagcg gcgtcgcttc
     2701 cgtaatcctc gggatcatga tatggggcga attccccgcg agcagcgcct tcataatcgg
//
LOCUS       DASSJH010000089         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_560960, whole genome shotgun sequence.
ACCESSION   DASSJH010000089 DASSJH010000000
VERSION     DASSJH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560960"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2257)
                     /gene="mutS"
                     /locus_tag="VFJ67_01895"
     CDS             complement(<1..2257)
                     /gene="mutS"
                     /locus_tag="VFJ67_01895"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET7289162.1"
                     /translation="MPPQTPMLRQYLAMKKEYPDSILFFRLGDFYEMFYEDAKVASKI
                     LGIALTSRNKSDKNPVPLCGVPHHSAEPYITKLLKSGHKVAVCEQIEDPKSAKGVVKR
                     KVVRVLTPGAVLDSENLDSKSNNYLASVYAGNGSYGLAYTDISTGIFRTTVFGTLEEL
                     MDEIYQIEPKEILLEDVSGDDGALAGSFRKSSSPLVTLLNSWVWDRDRARELLLEHLS
                     SKTLDAFGLEDRPEAVAASGALIQYLRDTQMEDMPPLDEPVFYEKSGSLLIDDTSRKN
                     LELLRSAGGDTRGSLLWVLDETMTAMGGRLLRQWISSPLLDAGGIEERLDAVGELKGK
                     AGVRADIRSTLKNISDIERLTGRIATSSARPRDLGALRDSSRYIKGVKTALQGAGSEI
                     LKHIAGTLDDLSDIREILESTLIDEPPVSARDGGIIREGVSGELDELRSLRRDGRKWI
                     AELEARERSASGINSLRVSYNRVFGYYIEVSKANLSLVPERFIRRQTLANAERYVTEE
                     LKEYEEKILGAEDRILAIERELFEGLRKKVADQAERIRRASSLIAAVDVLCALSEAAE
                     KYSYVRPELAASGVIELKNNRHPVVERMDLGERFVPNDIRLDADENQFLIITGPNMAG
                     KSTLIRQVALTVIMAQMGSFVPSTHARIGITDRIFTRVGASDNLARGHSTFMVEMVET
                     AYILRHATGKSLVILDEIGRGTSTFDGMSIAWAVAEYLHDRGAMTLFATHYHELAGLA
                     TSKRRVKNYNIY"
     gene            2467..>3276
                     /locus_tag="VFJ67_01900"
     CDS             2467..>3276
                     /locus_tag="VFJ67_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7289163.1"
                     /translation="MIKPNPFVKDLIPYAPGKPVEELERELGITGAIKIASNENPVGP
                     SPLALEAARKALGNIHRYPDGDAFYLKHKLSGILGTKPERLIFGNGSNDVLDIAARTF
                     LSPGDEAVMGEFAFIVYPIVTKAVGAKAVISPMPDHNHNLRDMHSRITPRTKAVFIAN
                     PNNPTGAMVKRSELEWFLDNVPDDLLVVIDEAYFEYVEDQEYPDSLSYQDTGKSILTV
                     RTFSKIYGLAGLRIGYGVADEGMVSAMQRARHPFNTSSVAQEAAMAALDDHE"
BASE COUNT          664 a          981 c          864 g          767 t
ORIGIN      
        1 cgtatatatt gtagttcttg acccttctct tcgacgtggc cagccccgcg agttcgtggt
       61 aatgcgtcgc gaaaagggtc atcgcccccc tgtcatgaag gtattcggcg accgcccacg
      121 ctatgctcat tccgtcgaac gtgctcgtcc ctctgcctat ctcgtctagt atgacgagac
      181 ttttgccggt cgcgtgcctg agtatgtacg cggtctcgac catctccacc atgaacgtcg
      241 agtgccccct ggcgaggttg tcggatgcgc cgaccctcgt gaagatcctg tccgttatac
      301 cgatccttgc gtgtgtcgag ggaacgaagc tccccatctg ggccataatc acggtaagcg
      361 caacctgtct gatgagcgtc gatttacccg ccatgttggg gcccgtgata atcaggaact
      421 ggttctcgtc cgcatcgagt cgtatatcgt tcggcacgaa acgctctccg aggtccatcc
      481 tctcgacgac ggggtgcctg ttgtttttga gctctatgac gccggacgcg gcgagctcgg
      541 gcctcacgta tgagtacttc tcggcggctt cagaaagcgc gcacaggacg tcaaccgccg
      601 ctatgaggga agaggcacgt cttattctct ccgcttgatc ggccaccttc ttccttaatc
      661 cctcaaagag ctctctttcg attgcgagaa tcctgtcctc ggcaccgagg atcttctcct
      721 cgtactcctt cagctcttcc gttacatagc gctcggcgtt ggccagggtc tgccttctga
      781 tgaatctctc gggaacgagg gagaggttcg ctttcgatac ctctatgtaa tagccgaaca
      841 ccctgttgta gctcaccctg agagagttta ttccggaggc gctcctctcc ctcgcctcga
      901 gctccgctat ccactttctg ccgtcccgcc tgagagacct gagctcgtcg agctctccgc
      961 tcaccccttc ccttataatc cccccgtcac gcgcggaaac aggcggctcg tcgatgagcg
     1021 tgctctctag tatctctcta atatcgctca agtcatcgag ggtcccggct atgtgtttca
     1081 atatctcgga gccagcgccc tgaagcgctg ttttgactcc ctttatatac cgtgaggagt
     1141 ccctgagcgc cccgaggtcc ctcggccggg ccgaggaagt tgctatcctt ccggtgagcc
     1201 tctctatatc gcttatattt ttgagcgtgc ttcttatgtc cgccctcacc ccggcctttc
     1261 ccttgagctc tccgaccgca tcgagcctct cttctatccc gcccgcatcc agaagtgggc
     1321 ttgatatcca ctgcctgaga agcctcccgc ccatggcggt catcgtttca tccagcaccc
     1381 agagcaaaga ccctcgcgta tctccgcccg ccgacctcag cagctcgagg ttctttctcg
     1441 aggtgtcgtc tatcaggaga gaccccgatt tttcatagaa tacgggctca tcgagtggag
     1501 gcatgtcctc catctgagtg tctctcaggt actgtatgag cgctcccgat gcggctacag
     1561 cttcgggtct atcttcgagt ccgaacgcgt cgagcgtttt cgaggacagg tgctcgagta
     1621 aaagctcacg cgcccggtca cggtcccata cccacgaatt cagcagagtg acgagggggc
     1681 ttgaggactt cctgaacgag cccgcgagcg cgccatcgtc ccccgatacg tcttcgagaa
     1741 gtatctcctt gggctctatc tggtatatct cgtccatgag ctcttccaac gtgccgaata
     1801 cggtagtcct gaagatacct gtcgatatat ccgtatacgc gagcccgtaa gagccgttcc
     1861 cggcataaac gctcgcgaga tagttatttg atttggaatc gaggttctcc gaatcgagca
     1921 cggcccccgg agtgagcacc ctgactacct tccttttaac cacacccttc gccgatttag
     1981 ggtcctcgat ctgctcgcag acggcgacct tgtgcccgct cttgaggagc ttcgttatat
     2041 acggctcggc gctgtgatgg ggcaccccgc ataacgggac cggatttttg tccgacttgt
     2101 ttctggaggt gagcgcgatg cccaatattt tggacgcgac cttcgcgtct tcataaaaca
     2161 tctcgtagaa gtccccgagt ctaaaaaaga gaatagagtc gggatactcc tttttcatgg
     2221 ccagatactg cctgagcatc ggggtttgag ggggcatgga tcagtagatt atatcgactg
     2281 gtgacctaac tctcaatttg cgtccgggca cacgggcgcc tgcgcaggaa ggaatacgaa
     2341 aacagagggg gctccccgcc cgtccttgcc tcatatgagc gcaggtgata gattaattcc
     2401 ttttattgcg ggagacgggc ttcatatgat tcaaatgcac atattcaggc attcgaggga
     2461 aacgaattga taaaacccaa tccttttgta aaggacttga taccctatgc ccccggtaaa
     2521 ccggtagaag agctagagag ggagctaggc atcacgggcg ctataaagat tgcgtcgaac
     2581 gagaacccgg tcggcccctc gcctctcgcc ctcgaagcgg cccgcaaggc tcttggcaac
     2641 atccaccgct acccggacgg ggacgctttt tatctcaagc acaagctctc gggcattctc
     2701 ggcacaaagc ccgagagact catattcggg aacggctcta acgacgtgct cgacatcgcg
     2761 gcaaggactt tcctctcccc cggggacgaa gccgtcatgg gggagttcgc gttcatcgtg
     2821 tatccgatag tgacgaaggc ggtcggcgca aaggctgtaa tctcgcccat gcccgatcac
     2881 aatcataacc tgagagacat gcactcgcgc atcacaccgc gcacgaaggc cgtcttcata
     2941 gcgaatccca ataacccgac gggcgctatg gtaaagagga gcgagctcga atggtttttg
     3001 gataacgtcc ccgacgacct cttggtcgtg atagacgaag cctacttcga gtacgtggag
     3061 gaccaggagt acccggattc cctgtcatat caggacacgg gcaaatccat tctcaccgtg
     3121 aggacgttct ccaagatata cgggctcgcg gggctcagga taggttacgg cgtcgccgat
     3181 gaaggcatgg tatccgcaat gcagagagcg cggcatccgt tcaatacaag ctccgtcgcg
     3241 caggaagcgg caatggcggc actggatgat catgag
//
LOCUS       DASSJH010000090         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_22140, whole genome shotgun sequence.
ACCESSION   DASSJH010000090 DASSJH010000000
VERSION     DASSJH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22140"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..395)
                     /locus_tag="VFJ67_01905"
     CDS             complement(162..395)
                     /locus_tag="VFJ67_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017294594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289164.1"
                     /translation="MKIDTYTKIILTLIALALWGIFLYPFFETKESDATTGIIDVNLK
                     QIDGSSIDRVLDVNIKELNGSSVYNSLPVEIKQ"
     gene            699..1685
                     /locus_tag="VFJ67_01910"
     CDS             699..1685
                     /locus_tag="VFJ67_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009545376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289165.1"
                     /translation="MRGLLSAFVFACLIVTISYGVQPVFAKQAVSASADGQCRLYNEK
                     SNNYKYEGNCTIKQNTSSGANEYDIKLGSGESYRFIQQGSTYKVQTPEGLSDHMATMT
                     DSGNVATFEWYKWKLTAEVTESSEPAPASNTKASASGSAQGTVQGLSDLVGAKAGQAE
                     STVEQRGYKYVKTDKSGEMSFSYWRESGSNKCVSIKTEDGRYQAIVYTPDFNCGNAAA
                     AETKASSTERAGQGQFDATGNIPCAQSKGQPMGQCPFGVAREGIGTAAVSVTMPDGRK
                     RMIFFENGKPTSADLSQADGNMNFSYTKESDLYMIRAGNERYEIPEVVVYGD"
     gene            2228..>2418
                     /locus_tag="VFJ67_01915"
     CDS             2228..>2418
                     /locus_tag="VFJ67_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7289166.1"
                     /translation="MRTLVVGDIHGNLKALRQAFERSGFNPRKDRLVSLGDVFDGHPH
                     SAECVELLMKVKDLVWCLG"
BASE COUNT          579 a          590 c          717 g          532 t
ORIGIN      
        1 agcggatatg aatattacga ataataaggc ggttataaac gggaagagat accgaggtaa
       61 agtgcgcata tcctgacatc ctccgggatg ttgaattccc atgattatac cccgcgaacc
      121 ccgccgattc agcaaagagc cgccccgggg cagcagggcg ctcactgctt gatttcgacg
      181 ggcagggagt tgtaaaccga gctgccgttc agctccttta tgttcacgtc gagcaccctg
      241 tcgatgctcg agccgtcgat ctgcttgagg ttgacgtcga tgatccctgt cgtcgcatcg
      301 gactccttcg tctcgaagaa cgggtagagg aatatgcccc agagcgcgag ggctatgagg
      361 gttaggatta ttttcgtgta cgtgtctatt ttcatgtgat gcctccgggg agatagttta
      421 acccggatcg gggagtttgt tgtccgatag ggcgggacgg attcgcgacc cgacgttgcc
      481 ggctcatttt ttcggagcga cgggcacatg ctgttagtcg atatgagaat accgtgttga
      541 agctgtgaag aggatttaag agaatgaatc caaggggact acagtttatc atattttata
      601 gatattaatc ataaacatct tgttttggga aatattcctt ggatatacta ttaaactagc
      661 tacactaaat acataaataa tagagggagg tataggttat gaggggctta ttatccgcgt
      721 ttgtctttgc ttgtttgatt gttacgattt cttacggtgt ccagccggta ttcgcgaagc
      781 aggctgtttc ggcctcggcc gacggacaat gcaggcttta caatgagaag agtaataatt
      841 acaagtacga ggggaactgc actataaagc agaatacgtc gagcggcgcg aatgagtacg
      901 atattaaact ggggagcggc gaatcatatc gttttataca gcagggatcc acttataagg
      961 tccagacacc tgagggattg tcggaccaca tggcaaccat gaccgatagc ggcaatgtag
     1021 ccactttcga gtggtataaa tggaaactta ccgctgaggt gacagagtca tccgagcccg
     1081 cgccggcatc gaacaccaaa gccagcgcat ccggaagcgc gcagggaacg gttcagggat
     1141 tgtccgacct cgtaggtgcg aaagcggggc aggccgagag cacggttgag cagaggggct
     1201 acaaatacgt caaaaccgac aagtcgggag aaatgtcctt cagctactgg agggagagcg
     1261 ggtcgaacaa gtgcgtttcg ataaagaccg aggacggccg ctaccaggcg atcgtttata
     1321 cgcctgattt caactgcggg aatgccgcgg cggccgagac gaaagcctct tccaccgagc
     1381 gcgcggggca gggccagttc gacgctacgg gcaacatccc gtgcgcacag tcgaagggac
     1441 agccgatggg ccagtgcccg ttcggagtgg cgcgtgaggg catcggcacg gccgccgtgt
     1501 ccgtaacaat gcccgacggg cggaagcgta tgatcttctt cgagaacggc aagcccacga
     1561 gcgcagacct gtcgcaggcg gacggcaaca tgaacttcag ctatacgaag gaatccgacc
     1621 tctatatgat cagagccggt aacgaacgct acgagatacc ggaggtagtt gtttacggag
     1681 actgaagcac tagtgaaccg gtacgaccgg ataattcgca ctctgatcct gttgtttgca
     1741 gcgctgctcg cgggatgcgg tctcgcgcat tcgatttcgt gcggatgaac gtatctactt
     1801 tctaacgtcg cgccttagcg gagcagacgc ggatacgccg gtgacaagcc atggaactac
     1861 ttgcctcgtt gttcgtcagg aaaccgctga acattttagc ggtggccttc ctgttcatag
     1921 cggggtacgc cgcgatgcgg tcattgaagc gcggcggcga acgccgtccc ccgcagcttc
     1981 tccttgcggc ttcagcgtgg atattatatg cggcctggga gttaatggtc cgcagccgta
     2041 ccccggaagc ggacatccgg gttgatcttc ttttaatctg gcctgccctc gcggtaatct
     2101 ccgcatggtc cctgtatcgt tcgctgagac cgggaagccg cctcgttcga aaacaaaatt
     2161 gatcgaaggg cggcggaaga aaatataacc gtttgcaaat acccgctgga atgagaataa
     2221 tgcttggatg aggacgctcg tcgtcggcga catacacggg aatctgaaag cgttaaggca
     2281 ggcgttcgag cgctcgggat tcaatccaag aaaggacagg ctcgtctcgc tcggggacgt
     2341 gttcgacggc catccgcact cggccgaatg cgtcgagctg ctaatgaagg tgaaggacct
     2401 cgtctggtgt ctcggcaa
//
LOCUS       DASSJH010000091         5289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_560971, whole genome shotgun sequence.
ACCESSION   DASSJH010000091 DASSJH010000000
VERSION     DASSJH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560971"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFJ67_01920"
     CDS             <1..230
                     /locus_tag="VFJ67_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HET7289167.1"
                     /translation="KICGWALARAHAKAGDTCMIAGYLGSSDSFDDALVKYAQAYADQ
                     VERDYEAFRKAIRSGRLHTDTDESSNLSFLL"
     gene            complement(302..766)
                     /locus_tag="VFJ67_01925"
     CDS             complement(302..766)
                     /locus_tag="VFJ67_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289168.1"
                     /translation="MTVKDIVLNAVSPSPLYLVELPDTKCRIIRNNGSKVYCGVYPLM
                     GGLFRKDVGETKRFGRRLCDTVAEKMNNHGFFTTDETPEYGISYGLSGKEYEYIYERT
                     GASKDRHLVVMFAYPEREALETQTCFEEIMRREYLKYRIEDSGAGSGLEFVY"
     gene            complement(920..1537)
                     /locus_tag="VFJ67_01930"
     CDS             complement(920..1537)
                     /locus_tag="VFJ67_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HET7289169.1"
                     /translation="MSSKFQFWYDFASPYSFLSAMRIDGLSTAAGVRVEWRPFLLGPI
                     FKSSGWDTSPFNIYEAKGRYMWRDVGRRSAKYGVSFRRPSEFPRNGVLASRIAIAFES
                     NERRALYSKLVFSANFTEDRDISDESVLASLLESLGEGPGRIVSAALSQYNRGRLRLA
                     TEEAAGKGIFGAPSFLAGEELFWGDDRLEDALEWFMRSVESADGR"
     gene            complement(1652..2140)
                     /locus_tag="VFJ67_01935"
     CDS             complement(1652..2140)
                     /locus_tag="VFJ67_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HET7289170.1"
                     /translation="MLSKEIQDALNNQIKNEYFASYTYLSMAAHCESINLRGFAKWLR
                     KQSSEELVHAMRLFDYVIDRDGHVILQAIEKPQSKYKSLSDMFQQVLDHEREVTGMIN
                     KLYEKAINENDHATAVELHWFIHEQVEEEKNATEILDKLKFAGDNSSAILLLDGQLGQ
                     RG"
     gene            2474..3757
                     /locus_tag="VFJ67_01940"
     CDS             2474..3757
                     /locus_tag="VFJ67_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7289171.1"
                     /translation="MTDIAGAKEKKREVLSWCLYDWAYSSFATTVISAVLPVYYSQVA
                     ARNLTGNTATVYWGYTTAVALIASVLLAPVMGAIADFSGIKKRLLLVFAAVGIFSTAL
                     LYYVTTGDWLLASVLFIFGNIGFSMSEVFYNALLPGIANAQEIDRISVKGYALGYLGG
                     GILLALDIGLIQIMEDKSLATRITFITVSVWWAVFTIPLIMNVKEPVVRSRKKIDTGI
                     LTAGFRRLSVTFAELRKYRELFLFLAAFWIYNDGIGTIIKMAAIYGAEIGISQSALIG
                     ALLMTQFVGIPFSFAFGRLAGYIGAKNSIMLGLFVYTIISISSYFMETALHFFILAFL
                     VGTVQGGTQALSRSLYGSMLPRDKTAEFFGFYGMSSKFAGIIGPLAFAIVSQATGTSR
                     LSIISLIVFFIAGAIILSRVDIQKGISASRDNSPV"
     gene            4076..>5289
                     /gene="dnaA"
                     /locus_tag="VFJ67_01945"
     CDS             4076..>5289
                     /gene="dnaA"
                     /locus_tag="VFJ67_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET7289172.1"
                     /translation="MAAKRTPLKHNLRTLFTEKKDDGKGLEFFISQSSRTAKHHQASE
                     DPAPGNIVWDRVLERIKKKSNPQVFFWFAPLKPLSETEDSVTLRANSEFDKDWINNHY
                     LEFIGDTIREIYGKTVSVKIITEQGRQTPREDKRAANDPGERKDAEPGKLFTGFLNPN
                     YSFERFIVGPSNQFAHAASTAVARKPGEAYNPLFIYGGVGLGKTHLINAIGNYIIKST
                     SNMARVCCISAEHFTNQVINSIKSNKMEDFRNRYRFGCDVLLIDDIQFIAGKESTQEE
                     FFHTFNTLYESKKQIVLTSDKSPKDMSYLEERLRSRFEWGLITDIQPPETETRIAIIK
                     NKAESGGISLPNEVALYLAQNTTSNIRELEGTLTNIIAHAKLLKTEITPDLAKEVLRT
                     ILKQQDKKFLSI"
BASE COUNT         1334 a         1423 c         1290 g         1242 t
ORIGIN      
        1 caaaaatatg cggctgggcc ctggcgcggg cccacgccaa agcaggtgat acgtgcatga
       61 tcgcgggata ccttggatcc tcggacagct ttgacgacgc ccttgtaaag tacgcacaag
      121 cttatgcgga ccaggtggag cgcgactatg aagccttcag gaaagcaata cgttcgggcc
      181 gtctccatac tgatacagac gaatcctcaa atctcagttt cctgctctga ctttctttca
      241 accggaagtt gtgttcatta aaagatatga atccgcggat cattaatatg atctgaatgt
      301 gtcagtaaac gaattcaagg ccggagccgg cgcccgaatc ctcgatcctg tatttcaaat
      361 actcccttct cataatctcc tcgaagcagg tttgcgtttc aagggcttcc ctttcgggat
      421 aggcgaacat cacgacgagg tgacggtctt tcgaagcccc cgtcctctca tatatgtact
      481 cgtattcctt cccggataat ccgtaggata tgccgtactc gggcgtttca tccgtcgtga
      541 aaaatccgtg gttattcatt ttctcggcta cggtatcgca tagtctcctg ccgaacctct
      601 tcgtctcccc gacgtccttt ctgaagagcc cgcccataag cggatacacc ccgcagtaaa
      661 ctttggaacc gttgttcctg ataatcctgc attttgtatc cggcagctcc acgagataaa
      721 gcggagaagg ggaaaccgcg ttcaggacta tatcttttac ggtcatcgga tttacctccg
      781 gcggattaat tcatgtagat gcatattata ccggatggac tccggataca aaatgaacgc
      841 gcgggtccac tcctttgtaa cagaggcaga agtacagccc gctggaattg tcatcaggtc
      901 tgactaccat cttcttcgtt caccggccgt ctgcgctttc gaccgatctc atgaaccatt
      961 cgagagcgtc ttcgagcctg tcgtcgcccc agaaaagctc ctccccggcg agaaagctcg
     1021 gcgctccgaa tatacccttt ccagccgcct cttccgttgc gagcctgagc ctgcctctat
     1081 tgtactgaga gagggcggcc gagacgattc ggcccggccc ctcaccgagg gactccagca
     1141 acgacgctaa cacggactca tccgatatat ccctgtcttc cgtgaaattg gcgctgaata
     1201 cgagttttga atatagggcg cgccgttcat tggattcaaa ggcgatcgcg atcctcgagg
     1261 cgagtacccc gtttcttgga aactcagagg gccttcggaa cgaaaccccg tatttcgcgg
     1321 agcgccgccc gacgtcccgc cacatgtagc gacccttggc ctcgtagata ttaaacgggg
     1381 atgtatccca gccgctgctc ttgaagatcg ggcctaggag aaacggacgc cactcgaccc
     1441 tgacaccagc ggctgtggag agtccatcta ttctcatagc ggaaagaaaa gagtaagggc
     1501 tcgcgaaatc gtaccagaat tggaatttcg atgacaagtt gattcccgga cgtatagaca
     1561 cgactcggtt ctatccaggc ttcttcttac gttacgccat tcggacagga cacggtcggg
     1621 aaaattcaga tgtcgcaaca gtcacccggt cttaacccct ctgtcccagc tgaccgtcga
     1681 ggagcagtat ggcggagctg ttgtctcccg cgaacttgag cttatccaga atctcggtcg
     1741 cgttcttttc ctcttccacc tgctcgtgta taaaccagtg gagctctaca gccgtcgcgt
     1801 gatcgttctc gtttatagcc ttttcgtaga gcttatttat catccccgta acttcccttt
     1861 cgtggtcgag cacctgctgg aacatatcag agagggattt atatttcgac tgcggtttct
     1921 ctattgcctg caggatcaca tgaccgtcac ggtctatgac ataatcgaag agcctcatcg
     1981 cgtgcactag ctcctcgctg ctctgtttgc ggagccactt cgcaaatccc ctcagattaa
     2041 tcgattcgca gtgagccgcc atcgagagat acgtgtacga agcgaaatat tcgttcttta
     2101 tctgattgtt aagcgcatcc tgaatttcct tgctgagcat ttatccctcc tggattgtaa
     2161 gtattatcga gcttgagacc gcttcaaaac actcagcggc caatacattg acgctaaaat
     2221 tatctcacaa acagggccta aattcaagta attatcattt gggaatgatt accatttaac
     2281 ccgtctaact gttaacctta agctattctt atatatttat agccgttagt gcggtcaata
     2341 atagatgtga tcctaatatt caggcagccg aaccaatcgc ggcccaatct tcacttttcg
     2401 agcgtttcct atgataatac tggacggaaa aggagtctaa tcgacaggca atcccgaacg
     2461 agtcggtaaa aaagtgacag atatcgccgg agccaaggaa aagaaaagag aggtcctgag
     2521 ctggtgcctc tacgactggg cctactcttc gttcgccaca accgttattt cggccgtcct
     2581 gccggtctat tacagccagg tcgcagcccg aaacctcaca ggcaatacgg ccaccgtgta
     2641 ctggggctat acgaccgcgg tagcgctcat cgcgtcggtt ctacttgccc ccgtaatggg
     2701 cgcaatcgcc gatttttcgg gaatcaagaa aagactcctg ctcgtattcg cggcggtcgg
     2761 gatattctca acggccctcc tctattacgt gacaacgggc gactggctcc tggcttccgt
     2821 cctgttcatt ttcggcaaca tagggttttc gatgtccgag gttttttaca acgctctcct
     2881 gcccggtata gcaaacgctc aggagatcga ccgtatttcg gtgaaggggt acgccctcgg
     2941 atacctggga ggcggcatat tgctcgccct cgacataggt ctcatacaaa tcatggagga
     3001 taagtcgctc gcgacccgca tcacattcat aaccgtgtcg gtctggtggg ctgtcttcac
     3061 gatcccgctc atcatgaacg tgaaggagcc tgtcgtgagg tcgcgtaaaa agatcgacac
     3121 cggaattctc acggcaggct tcagacgctt gtcggtaacc ttcgccgagt tgagaaaata
     3181 cagggagctg tttctcttcc tggccgcctt ctggatttac aacgacggaa tagggacgat
     3241 aatcaagatg gcggcgatct acggagccga gatcggcatc agtcagtcgg ccctcatcgg
     3301 agcgctgctc atgacacagt tcgttggcat acccttctcc ttcgcgttcg gcagactggc
     3361 cggatacatc ggcgcaaaga actcgataat gctcgggctc tttgtctaca ccataatttc
     3421 catatcaagt tatttcatgg agacagctct ccacttcttc atcctggctt tcttagtcgg
     3481 aaccgtacag ggaggcacgc aggcgctgag caggtccctt tacggctcga tgctgcctag
     3541 ggataagacg gcggagttct tcggattcta cgggatgagc tccaagtttg caggcatcat
     3601 cggacccctc gcattcgcca tcgtatcgca ggcaacgggg acgagcaggc tcagcattat
     3661 ttcgctgatt gtcttcttca tcgcgggcgc aattatactg agccgcgtcg atatacaaaa
     3721 aggaataagc gcatcgcgcg acaatagccc tgtttaaagg gacttccgcg ttcgctgttg
     3781 aaaaataccg atttcgcgag attcaaaact caatattaac aggatggtta cagagacact
     3841 ccacacttcc gcacacagcc gcaacacgca tcggcatgct caacgaaatt gtgtaacgaa
     3901 ccgcaatcga aggataataa aataatactt gacatcatat ttttgcatct gttaggatgt
     3961 aacctacgga tctgggtggg gcttaattca cgtattaaac ctgactaaat aactatccac
     4021 acggtgttgg tgttttgtgg ataagttgtt tcttgatttg gaggagcaga ggacattggc
     4081 cgctaaaaga acgcccctga aacataatct taggacactg tttactgaaa aaaaagacga
     4141 cggaaaggga ctcgagtttt tcatctcgca atcgtcgcgc accgccaagc atcatcaggc
     4201 gagcgaagac ccggcgccgg ggaatatagt ctgggaccgc gtgctcgaaa gaataaaaaa
     4261 gaaatccaac ccgcaagtgt ttttctggtt tgcgccgtta aagcctctgt ccgaaacgga
     4321 agattccgtc actctcaggg ccaacagcga attcgacaag gactggatca acaaccatta
     4381 tctggaattc ataggcgata ccataagaga gatttacggg aagaccgtat cggtgaagat
     4441 tattaccgag cagggcagac agactccccg ggaagataaa agagccgcga acgatccggg
     4501 agagcggaaa gacgccgaac ccggcaagct tttcaccggg ttcttaaacc ctaactattc
     4561 gttcgaacgc ttcatagtgg gcccgagcaa ccaattcgcc cacgcggcct cgacagcagt
     4621 cgcgagaaag ccgggagagg cctacaaccc tctcttcatt tacggaggcg tgggtcttgg
     4681 aaaaactcat ctcataaacg cgataggcaa ctacatcatc aaatccacgt caaatatggc
     4741 gagagtatgc tgcatttcgg ctgagcactt cacgaaccag gtcatcaaca gcatcaagtc
     4801 caacaagatg gaggacttca ggaaccggta caggttcggc tgcgacgtgc tcctcatcga
     4861 cgacatacag ttcatcgccg gaaaggagag cacgcaggag gaattcttcc acacgttcaa
     4921 cacgctctac gaatccaaga aacagatcgt gctcacgagc gataaatcgc ccaaggacat
     4981 gtcttatctc gaggagaggc taaggtcgag gttcgaatgg ggcctcatta ctgacataca
     5041 gccgcccgaa acggaaacga ggatagctat aatcaagaac aaggccgaat ctgggggaat
     5101 atcccttccg aacgaggtcg cgctctattt agcccagaat acgacctcca atatcaggga
     5161 gctcgaaggc acgctgacca acattatcgc ccacgcgaag ctcctgaaga cggaaataac
     5221 accggacctc gcaaaggaag tgctgaggac catactcaaa cagcaggaca agaagttcct
     5281 cagcataga
//
LOCUS       DASSJH010000092         6849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_346987, whole genome shotgun sequence.
ACCESSION   DASSJH010000092 DASSJH010000000
VERSION     DASSJH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346987"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..429
                     /locus_tag="VFJ67_01950"
     CDS             109..429
                     /locus_tag="VFJ67_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET7289173.1"
                     /translation="MTTKEKPSKTEEEYFAKEEAEKSKRLKEKVKHETEDEERERIKG
                     ICYMRCPKDGGTLNEVVFRGISIDRCSTCGGVWLDNGELEKLAGTEDKSVISDVLSFF
                     SGKK"
     gene            complement(437..901)
                     /gene="tadA"
                     /locus_tag="VFJ67_01955"
     CDS             complement(437..901)
                     /gene="tadA"
                     /locus_tag="VFJ67_01955"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET7289174.1"
                     /translation="MTKNDDERFMRLAIEQARIAGRMGEVPIGAVITDGADKVVSSGY
                     NLREAGNDPTAHAEIIAIKKAGETLGSWRLEGSVIYVTIEPCLMCMGAILLARIKRLV
                     FGARDPKAGAVFSVYEIGNDRRLNHTVEVTEGVCAEECSALLKDFFKTLRKS"
     gene            complement(939..2666)
                     /locus_tag="VFJ67_01960"
     CDS             complement(939..2666)
                     /locus_tag="VFJ67_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7289175.1"
                     /translation="MLLLFKPRDAKYLGTGKLPFGSFDNTAEWKPITKCLEMGAEQFP
                     EKTMFRVGNGEGEIVESYSYKETNDWANRVANGLKDEFGVKKGDKVGMYMINCSEYVA
                     SIVAIHKIGGVQVPINKDEKGERLAYVINYSEMVALVIDPGSLPFLEEISKDLKGLKV
                     IFMTGDSAKVPAEIGGIKTLPFSVFDKFPAKNPGVKVTTHDMERCMFTSGTTGMPKGV
                     ARNHGGVILTVRAYLQQQGVRSDDVLMSVLSLGHANAQVMCLFSAMGAGATAVFFPRF
                     SASSFWKWAGGCGANCVNMLGAVAEYLWAAEPSEWDRKHKVRIMLGSPAPRNLQEFQE
                     RFGVRVIDGYGSTEMGMVLWKDPEDHRPGSSGFSMEGYYVELRDPQDQSRVMRPFWDP
                     YEDPTPPDEAKGLLYIKPLVPHTTLNEYFKDARRTREAFDDDGFFNSDDLFARGIDGR
                     YYFVGRFSRIRVSGENVDPIAVQDFAMQYAAIQEAVAVGIRLPNVSDDEIKLNITLKQ
                     GEKFDPIEFSKWMAERVMVAMVPRFIEVYEDGFPVTATQKIKVAEIKEITDKTWDRSK
                     TGLKFSARK"
     gene            complement(2907..4628)
                     /locus_tag="VFJ67_01965"
     CDS             complement(2907..4628)
                     /locus_tag="VFJ67_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7289176.1"
                     /translation="MILLFKPREQQYIGKGSLPFGTFDNTKEWRPITKYLEEGAETFP
                     GKTMFRVADRDGNLRETFTYEETNSWANRVANGLINKYKIKKGDRVGIYMLNSAEYVI
                     SIIAVHKAGAVQVPINKDEKGERLAYIINYSEMKALVIDRESIQMVGQIAGELEHLEH
                     VFVTGEGDLPEDIGGIKTLPFASFGEFSGENPRLDVRASDVERCMFTSGTTGMPKGVS
                     RNHGGVILTVRGYIQQQGVRSGDVLMSVLTLGHANAQVMCLFTAIGAGATAVFYPRFS
                     ASSFWKWAAECGATCVNMLGAVAEYLWAAEPSEWDNKHGIRIVLSGPAPRNLKEFQER
                     FNTRAIDGYGSTEMGMVLWNEPEDTRPGSSGRPMEGYYVEIRDPQDTSKVMRPFWDSS
                     ETPKPPEEAKGLLFIKPLIPHTTLNEYFKDERRTREAFDDDGFFNSDDLFAQGIDGRY
                     YFQGRFSRIRVSGENVDPIAVQDFAMQYPAVQEAVAVGIRLPNVSDDEIKLNITLKKG
                     KQFDPVEFSKWMAEIAPVFMVPRFIEVYEGGFPVTATQKIRVAEIKEITDKTWDRNKT
                     GLKFRVR"
     gene            complement(4749..5333)
                     /gene="pabA"
                     /locus_tag="VFJ67_01970"
     CDS             complement(4749..5333)
                     /gene="pabA"
                     /locus_tag="VFJ67_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET7289177.1"
                     /translation="MILMIDNYDSFTYNLVHYLSELGEEVVVHRNDKITIEDVGKLNP
                     DMIVVSPGPCTPREAGISVDVIKEFAGRTPILGVCLGHQSMAYAYGADIVRAGRLLHG
                     KTSQIHHDGKGIFKGVPDPFEATRYHSLLVDRETLPECFEVSAWTDEGEIMGIRHKEY
                     PMEGVQFHPESILTKYGKDILRNFISIARGKSPA"
     gene            complement(5374..6849)
                     /gene="trpE"
                     /locus_tag="VFJ67_01975"
     CDS             complement(5374..6849)
                     /gene="trpE"
                     /locus_tag="VFJ67_01975"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET7289178.1"
                     /translation="MILPSLADFRKKLRKGNLIPVWKEVLADFDTPVSAFRKIESGDY
                     AFLLESVEGGEKWGRYSFLGTEPSVIFRSKGKDIEIIEDGKSKKKKGNPIDSLRELMS
                     RYKPVHTDELPRFHGGAVGYFSYDIVRFVEDLPDVKKDELGLWDSLFMITDSVLVFDN
                     VNHKIKIICNAYVSHPEKAEDAYNGAVERIEETISRLRKPLGFYESGGAGKNGGPELV
                     SNFKPEDYKEAVRKTKEYITAGDIIQAVISQRWKTKLDVDPFDLYRALRVLNPSPYMF
                     YLKTGTETLVGSSPEVMVRVEDGEVESRPIAGTKPRGKTEAEDRVLEEELLSDPKERA
                     EHIMLVDLARNDLGRISETGTVKVDELMVVERYSHVMHIVSNVISRLRADKDAFDVVK
                     ATFPAGTLSGAPKVRAMEIIEEMEPSRRGAYGGAVGYFSFSGNMDTCITIRTFVIKDG
                     QIFIQAGAGIVADSDPEREYQETVNKVKALVRAIEVAKSGL"
BASE COUNT         1327 a         2047 c         1711 g         1764 t
ORIGIN      
        1 acccctttga ccaagtgtca aacggaactg tcctatacgt aaacgggacc gaaattaatt
       61 atcgagagtc cggttttcaa tccataccgt taagacagga gaatgacgat gaccacaaaa
      121 gaaaagccct cgaagaccga agaagagtat ttcgccaagg aagaagccga gaagtcgaag
      181 aggctcaaag agaaggtaaa gcacgagacc gaggacgagg agagggaaag gataaagggg
      241 atttgttata tgaggtgccc taaagacggc gggactctga acgaagtcgt attcaggggt
      301 ataagtattg acaggtgcag cacatgcgga ggggtatggc tcgacaacgg tgagctcgag
      361 aagctcgccg ggacggaaga caagagtgtt atatccgatg tactcagctt tttcagcggg
      421 aagaagtaag agggctttaa ctttttctga gtgttttgaa gaagtcttta agaagcgcag
      481 agcattcctc ggcgcaaaca ccttccgtaa cctcgaccgt gtggttaagc ctccggtcgt
      541 tcccgatctc ataaacagag aatacagccc ccgctttcgg gtcgcgcgca ccgaagacga
      601 gcctctttat tctcgcgagt aatatcgcgc ccatgcacat caggcatggc tctatcgtaa
      661 cgtatatgac ggagccttcg agcctccagc tcccaagtgt ttcaccggct ttttttattg
      721 ctatgatttc tgcgtgtgct gtagggtcgt ttcccgcttc gcggaggttg tatccgctcg
      781 aaacgacttt atccgccccg tcggttatga cggcgccaat gggcacttcg cccatgcgcc
      841 cggctatccg ggcctgctcg atagcaaggc gcatgaatct ttcgtcgtcg ttcttcgtca
      901 tcgggaagcg aagcggattc tccgggcttc aggcgcgctc actttctcgc gctgaacttt
      961 agccccgttt tgcttctgtc ccaggtcttg tccgttattt cctttatctc tgcaacctta
     1021 attttctgag tggcggtcac cgggaacccg tcctcgtaga cttctataaa ccgcgggacc
     1081 atcgccacca tgaccctctc ggccatccac ttcgagaact ctatcgggtc gaacttctca
     1141 ccctgcttga gcgtgatatt cagcttgatc tcgtcgtccg atacgttcgg gagccttatg
     1201 cccaccgcga ccgcctcctg tatagccgca tactgcatcg cgaagtcctg aacggcgatc
     1261 gggtccacgt tctccccgga tacccttatc ctgctgaacc tgcccacgaa gtaatacctt
     1321 ccgtctattc ccctcgcgaa gaggtcgtcc gagttgaaaa agccgtcgtc gtcgaacgct
     1381 tcccttgttc tcctcgcgtc cttgaaatat tcgttgagcg tggtgtgcgg cacgagcggc
     1441 tttatgtaca gaagcccttt cgcttcatcg gggggcgtcg ggtcttcata cgggtcccag
     1501 aacggtctca tcaccctgct ctggtcctgc gggtcccgta gctccacgta atatccttcc
     1561 atcgagaacc cggacgagcc cgggcgatgg tcctcagggt ctttccagag caccattccc
     1621 atttccgtag agccgtaccc gtcgattacc ctcacaccga acctctcctg aaactcctgg
     1681 aggttcctcg gcgcggggga cccgagcatg atccttacct tatgtttcct gtcccattcg
     1741 gagggctctg cggcccaaag gtactcggcg acagcgccga gcatgttgac gcagttggcc
     1801 ccgcaccccc cggcccattt ccagaagctc gacgccgaaa agcgcgggaa aaataccgcc
     1861 gtcgcgccag cgcccatggc gctgaataaa cacatgacct gggcgttcgc gtgccctagc
     1921 gagagcacgc tcatgagcac gtcgtcgctc ctcacgccct gctgctggag gtatgcgcgg
     1981 accgtcagta tgactccccc gtggttcctc gcgacgccct tgggcatgcc tgtggtgcct
     2041 gaagtgaaca tgcatctttc catatcgtga gtagtgacct tcaccccggg gttcttcgct
     2101 gggaatttat cgaagactga aaacgggagc gtcttgatcc ctccgatctc ggccgggacc
     2161 ttcgccgagt cgcccgtcat gaatattact tttaagcctt ttaaatcctt ggagatttcc
     2221 tctaagaatg gcaggctccc cggatcgatg actagagcta ccatctccga atagttgata
     2281 acgtatgcga gtctctcgcc cttctcgtcc ttgtttatcg gcacctggac gccgcctatt
     2341 ttatggatcg ccacgatgct ggccacgtat tcggagcagt ttatcatgta catgccgact
     2401 ttatcgccct tctttactcc gaattcatcc ttgaggccgt ttgcaacccg gttggcccag
     2461 tcattcgttt ccttgtagga ataactctcg actatctccc cctctccgtt ccctacccga
     2521 aacattgtct tttcagggaa ttgctcggct cccatctcca gacacttggt gataggcttc
     2581 cattccgccg tattgtcgaa cgaaccgaac gggagttttc ccgtccccag atacttcgcg
     2641 tcgcggggtt taaagagtaa aagcataaca accctccttt gtggtggcta aattgatttc
     2701 ccggtttttc ggaaatttga ctctattaca ataagaaaat aggagaaatt tgtcaacctg
     2761 atatttcctg cggaattctg aggataaatt tatcatttca ttgcacattt atcccgccgg
     2821 gagatatgtg taaaccgccg cgcgggcaca cgatttaata tcctctcaaa caggtgagtc
     2881 gcattctgtt catcgctcaa gagaggtcag cggaccctga acttgagccc ggtcttattc
     2941 ctgtcccagg tcttgtcggt gatctccttt atctcggcga cccttatttt ctgtgtggcg
     3001 gttacaggga accccccttc atagacctcg atgaacctcg gcaccatgaa tacgggcgct
     3061 atttcggcca tccacttgga gaattcaacc ggatcgaact gcttaccttt ctttagagtt
     3121 atattcagct tgatctcgtc gtccgatacg ttcggcagcc ttattcccac ggccaccgcc
     3181 tcctgtacgg ccgggtactg catcgcgaag tcctggaccg cgatcgggtc cacgttctcg
     3241 cccgaaaccc tgatcctgct gaaccttccc tgaaagtagt acctgccgtc tatcccctgc
     3301 gcgaacaggt cgtcagagtt aaagaatccg tcgtcgtcga acgcttccct cgtcctccgc
     3361 tcgtccttga agtattcatt cagcgtcgtg tgcggtatga ggggttttat gaagagaagc
     3421 cccttggcct cctcgggcgg cttcggtgtt tccgatgaat cccagaaagg acgcatgacc
     3481 ttcgacgtat cctgggggtc cctgatctcg acgtagtatc cctccatcgg acggccagac
     3541 gagccgggtc tcgtatcctc gggttcgttc cagagcacca tgcccatctc cgtcgagccg
     3601 tacccgtcta tggccctggt gttgaagcgc tcctgaaatt ctttcaggtt cctgggggcg
     3661 ggtcccgaga gtacgatcct tattccgtgc ttattatccc attcggaggg ctccgcggcc
     3721 cagaggtact cggctactgc acccagcatg ttgacgcacg ttgctccgca ttcagcagcc
     3781 cacttccaga agctcgaggc cgaaaaacga gggtagaaca ccgcagtcgc ccccgcgcct
     3841 atggcggtga agagacacat cacctgcgcg ttcgcgtgcc cgagcgtgag cacgctcatg
     3901 agcacatccc cgctcctcac cccttgctgc tgtatgtatc cgcgcactgt cagtattacg
     3961 ccgccgtggt tcctcgacac acccttcggc atgcctgtcg tgccggaagt gaacatgcag
     4021 cgctcaacgt cggatgccct gacgtcgagc cgcgggttct ctcctgaaaa ttccccgaag
     4081 ctcgcgaacg gtagggtttt aatgccgccg atatcctccg gcaggtcgcc ttctcctgtg
     4141 acgaaaacgt gttcgaggtg ttcgagctcg cccgctatct gcccgaccat ctggatgctt
     4201 tcccggtcta tgacgagcgc cttcatctcg gagtagttga taatgtacgc gagcctttcg
     4261 cctttctcgt ctttattgat cgggacctgc accgcccccg ccttgtgtac ggcgattata
     4321 gagattacgt attcggcgga gttcagcatg tatatgccga ccctgtcccc cttcttgatt
     4381 ttatatttat tgatgaggcc gttcgcgacg cggttcgccc aggagttcgt ttcttcgtag
     4441 gtgaacgtct ctctcagatt tccatccctg tccgccaccc tgaacatcgt tttaccggga
     4501 aaggtctcgg cgccttcttc gaggtactta gttatgggcc gccactcctt cgtattgtcg
     4561 aatgttccga aaggcaggct ccctttccct atgtattgct gctcacgggg tttgaaaaga
     4621 aggatcatat cccggctcct ggtcttattt tgaacgttta ggaatataaa gaaaatcgtg
     4681 tttatttgtc aaccggcgcg tgtttgttcg tgccgccgat cttgaagagg gcttgttacc
     4741 ggctttgact atgcgggcga ttttccgcgg gcaatcgata tgaagttcct gagtatgtcc
     4801 ttaccgtact tggtcagtat ggattcgggg tggaactgca caccctccat cgggtattcc
     4861 ttgtgcctta tccccattat ctctccctcg tccgtccacg cggatacctc gaagcactcg
     4921 gggagcgttt ccctgtcgac gagaagcgaa tggtacctgg tcgcttcgaa cgggtcgggg
     4981 acgcctttga aaatgccctt cccgtcgtga tgtatctgcg aggtcttccc gtgaaggagc
     5041 cttcccgccc tgacgatatc cgcgccgtag gcataggcca tcgactggtg gccgagacac
     5101 actccgagga tgggcgtccg gcccgcgaac tccttgatca cgtcgaccga tatgcccgct
     5161 tctctggggg tgcaggggcc gggggatacg acgatcatgt cggggttcag cttccccacg
     5221 tcctcgatcg taattttgtc gttcctgtgc acgacgacct cttccccgag ctcggacagg
     5281 taatggacga ggttatatgt gaacgagtcg tagttatcaa tcatcaatat catgtctcta
     5341 cccggttctg tattaaataa tgagtctttt tgttcagagc cccgatttcg caacctctat
     5401 cgcccttacg agggccttta ccttgttcac cgtctcctgg tattcgcgct ccgggtcgga
     5461 atccgcaacg attcctgctc ccgcctgtat gaatatctgc ccgtccttga tcacgaacgt
     5521 ccttatggtt atgcacgtat ccatgttgcc cgagaagctg aaatacccga cggccccccc
     5581 gtaagcgccc ctccggctcg gctccatctc ttctattatc tccatcgccc tcactttggg
     5641 cgctcccgaa agggtccctg cgggaaaagt cgcctttacc acgtcgaagg cgtccttatc
     5701 tgctcttagc ctggatatga cgttcgatac gatgtgcatg acgtgagagt atctctctac
     5761 caccatcagc tcatccacct ttaccgtgcc cgtctctgat atcctgccga ggtcgtttct
     5821 cgcgaggtct acgagcatta tgtgctctgc gcgttctttg gggtccgaaa gcagctcctc
     5881 ctcaagcacg cggtcttccg cctcggtctt ccccctgggc ttcgtccccg cgatcggtcg
     5941 gctttcgacc tccccgtcct ccacccttac catgacctcg ggcgacgagc ccacaagcgt
     6001 ctcggtgccg gtcttcaggt agaacatgta cggcgaaggg ttaagcaccc tgagcgccct
     6061 gtagaggtcg aagggatcga cgtcgagctt cgttttccac ctctgtgata tgaccgcttg
     6121 tatgatgtcc ccggccgtga tgtattcctt cgttttcctt actgcttcct tgtagtcttc
     6181 gggtttgaag ttggagacaa gctcgggccc gccgttcttt cccgcgccgc cgctctcgta
     6241 gaagccgagc ggcttcctca gcctgcttat tgtttcttct atcctctcga ccgccccgtt
     6301 ataggcgtcc tccgccttct ccgggtgaga gacgtacgcg ttgcagatta tctttatttt
     6361 atggttcacg ttgtcgaaga cgagaacgga gtcggtgatc atgaacagcg agtcccagag
     6421 cccgagctcg tcctttttca catcgggcag gtcctcgacg aacctcacta tgtcatagct
     6481 gaaatacccg accgctcccc cgtggaacct cggaagctcg tccgtatgga caggtttata
     6541 cctcgacatt agctccctca gagagtctat cgggttcccc ttcttctttt tcgacttacc
     6601 gtcctcaatt atttctatat cctttccttt ggatctgaaa atgacggaag gttccgtacc
     6661 gaggaagctg tacctccccc acttctcccc gccttcgacg ctctcgagga ggaacgcgta
     6721 gtcgcccgac tcgatcttcc tgaaagccga cacaggggta tcgaaatcgg ccagcacttc
     6781 cttccatacg ggtatgaggt tccccttcct taactttttc ctgaaatcgg ccagagacgg
     6841 taagatcat
//
LOCUS       DASSJH010000093         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_455225, whole genome shotgun sequence.
ACCESSION   DASSJH010000093 DASSJH010000000
VERSION     DASSJH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455225"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFJ67_01980"
     CDS             <1..357
                     /locus_tag="VFJ67_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET7289179.1"
                     /translation="IVLSLFTFFWVSGFDIIYATLDEEFDKKSGVRSMVSVYGKDTGL
                     YIAGLLHYAAFFCIAFIYFFMFKTVVSGALLAVIGILLYFEHKKSDNVDLAFFKVNIA
                     VGLAVFLFILAGIYLP"
     gene            357..908
                     /locus_tag="VFJ67_01985"
     CDS             357..908
                     /locus_tag="VFJ67_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HET7289180.1"
                     /translation="MRTIVGFTGASGAAYGVDFLRRCPDDKYLIASKWGKRVLHEELG
                     LKVEELRPWVSDIYSDSDLGAPFSSGSNNFDSLVIIPCSISTLAKIANGIADTLITRI
                     AQVALKERRRLVIAIRETPLSSIALENALKLSREGAIIMPISPPHYLKADTVNDLIKA
                     YVDKVLNIIGVGTGNGWKHDELD"
     gene            1035..2810
                     /locus_tag="VFJ67_01990"
     CDS             1035..2810
                     /locus_tag="VFJ67_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HET7289181.1"
                     /translation="MPKKQFYDLKEYINYLEAIGDLVRVKAEVDPILEITEISSRVIK
                     ERGPALIFENVKGASYPLAINLFGTEERVELALGRKPRDVGEELVELFHKVNPPSLKS
                     FFSVLPKAYDLLSMRTKSVRSGASQEVEERPDLTKLPIIKCWPLDGGRFITLGLVLTQ
                     DPVTNRRNLGIYRLQIYDEKTTGMHWHPHKGGAAHYHEAGKLGRDLEAAVVLGGDPKM
                     IFTAIAPLPEGMDELAFASYLRGKPIPMVKGRSISLPVPANAEFVIEGVVPRNELRIE
                     GPFGDHFGHYSMEAEFPVFHVSRITHILNPVYPATVVGKPPKEDVFLGMAAADMFSPL
                     ARIIHPEVKDMWAYPEAGFHNLLVVSVDERYPRNGVKAMLALWGMGQLLLTKVMITVS
                     SGVNPRDWNAVLAEIGENFDPGEDFLMIPWAPLDTLDFTSGKLNVGSKMGINAVRKKE
                     GGRNKRKAPDRVPDPREKHGEILDWRLLPGGILVLKLEKDPKEMLSKMFGTPGYEDVR
                     IIAIVSPDIDVHDDTELIWGIFTRFDPYLDVIFERTELRGSAVVYGGRMGIDATIKSW
                     YPLVIEMSEDVKETVTGRWKEYWTK"
     gene            2786..>3652
                     /locus_tag="VFJ67_01995"
     CDS             2786..>3652
                     /locus_tag="VFJ67_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7289182.1"
                     /translation="MERILDEVEKLCFDKELFPIINKVASGERLSFEDGLTVMNTPDL
                     NTVGMLADYVKRKRAGDKVYFVVNRHVNPTNICAISCKFCAFGTTKKSANAYELSHEQ
                     ILSLLSDDIREVHIVGGLHPEWEFRHYLDIVKLVKDHFPKTHVKAFTAVEIDWFTEIS
                     GMGLEEVLETLRETGVDALTGGGAEILHPDVRKKICAPKTVATRWEEIHGLAHRMGIP
                     TNATILYGHIEEPFHVVDHYERLRRIEDESPGFFAFIPVLFQPENTGLKKQVKFFPAS
                     YDLKIHALARLYL"
BASE COUNT          873 a          995 c         1054 g          730 t
ORIGIN      
        1 atcgtgctct cgttattcac gttcttctgg gtctccggtt tcgacattat ttatgccacg
       61 ctcgacgagg aattcgacaa aaagagcgga gtgcgctcca tggtttccgt gtacggcaag
      121 gataccgggc tctatatcgc cggccttctc cattacgcgg cctttttctg catagccttt
      181 atctacttct tcatgttcaa aaccgtagtg tcgggagcgc tgctggccgt cataggaatt
      241 cttctttatt tcgagcataa aaagtcggat aacgtggacc tcgcgttctt caaggtcaat
      301 atagccgtgg gcttagccgt cttccttttt atcctcgctg ggatatactt accctgatgc
      361 ggacaattgt aggattcacg ggcgcttccg gggcggcgta cggcgtcgat ttcctgagaa
      421 gatgcccgga tgacaagtat cttatagcga gcaagtgggg gaagagggta ttgcacgaag
      481 agctcggtct caaggtggag gagctgagac cgtgggtcag cgacatatac agcgactccg
      541 acctcggagc cccgttctcc tctggcagca acaacttcga ctcgctcgtc atcatcccct
      601 gttctatctc gacgctggca aagatagcta acggcatcgc cgatacgctc atcaccagga
      661 tagcgcaggt cgcgctcaag gaaaggagga ggctcgttat cgccatccgt gaaaccccgt
      721 taagctccat tgctttggag aacgcgctca agctctcgag ggaaggggcg atcataatgc
      781 ctataagccc gccccattat ctcaaagccg acacggtgaa cgaccttatc aaggcctacg
      841 tcgacaaagt gctgaacatt ataggcgtcg gtacgggtaa cggctggaag cacgacgagc
      901 tcgattagat aaaatatccg ttccgggtat attgccgggg gtcgacaaac atcttgcgca
      961 cctaatgtaa aaaatcaatt atgcgccggt tccgtcctga ttctaaataa tatacacgtc
     1021 aagaagtgtc tgaaatgccg aaaaagcaat tctatgacct gaaggaatac ataaactatc
     1081 tcgaagccat cggggacctc gtccgcgtaa aggcggaagt ggacccgata cttgaaatca
     1141 ccgagatttc atcaagggtg atcaaggaaa ggggcccggc cctcatattc gagaacgtaa
     1201 agggcgcttc atacccgctc gcgataaacc tgttcggtac ggaagaaagg gtcgagctcg
     1261 ccctcggcag gaagccccgt gacgtggggg aagagctcgt cgagctcttc cacaaggtga
     1321 accccccgtc gctcaaatcc ttcttctcag tcctgcccaa agcctacgac ctcctctcca
     1381 tgaggacgaa gagcgtcaga tcaggcgcgt cacaggaggt ggaggagcgc cccgacctga
     1441 cgaagctgcc gattataaaa tgctggcccc tagacggggg acgcttcata accctcggcc
     1501 tcgttctcac gcaggaccct gtgaccaaca ggaggaacct cggcatatac aggctccaga
     1561 tttacgacga gaaaacgact ggcatgcact ggcacccgca taagggcggc gcggctcatt
     1621 accacgaggc ggggaagctc ggccgggacc tcgaagcggc cgtggtcttg ggcggcgacc
     1681 cgaagatgat attcacggcc atagcccctc tccctgaggg tatggacgag ctcgctttcg
     1741 cgagctatct gagaggaaag cccataccga tggtgaaggg caggagcata tcgctcccgg
     1801 tcccggcgaa tgccgaattc gtcatcgagg gagtagtccc gcggaatgag ctaaggatag
     1861 aaggcccgtt cggcgaccac ttcgggcatt attcgatgga ggccgaattc cccgtattcc
     1921 acgtgagccg gataactcac atcttgaatc ccgtatatcc cgcgacggta gtgggcaagc
     1981 ccccgaagga ggacgtattc ctcggcatgg ccgcggccga catgttctcg ccgctggcga
     2041 ggataatcca ccccgaggtt aaggacatgt gggcctatcc cgaggccggg ttccacaacc
     2101 tgctcgttgt ctcggtcgac gagcgctacc cgcggaacgg cgtaaaggcg atgctcgcgc
     2161 tctggggcat gggccagctc ctccttacga aggtgatgat tacggtatcg agcggcgtca
     2221 atccccgcga ctggaacgct gtgctcgccg agataggcga gaacttcgat ccgggcgagg
     2281 acttcctcat gataccctgg gcccccctcg acacgctcga cttcacgagc gggaagctca
     2341 acgtcgggag taagatggga attaacgccg tgcgtaagaa agagggcggg aggaataagc
     2401 ggaaggctcc cgaccgtgtg ccggacccga gggaaaagca cggggagata cttgactgga
     2461 ggctcctccc gggaggaatc ctcgttctga agcttgaaaa ggaccctaag gaaatgctga
     2521 gtaaaatgtt cgggacgccc gggtatgagg acgtgaggat aatcgcgatt gtgagccccg
     2581 acatagacgt tcacgacgac acggagctca tatggggcat atttacgagg ttcgacccct
     2641 acctcgacgt gatattcgag cggaccgagc tgaggggctc ggccgtcgtt tacggcggca
     2701 ggatgggcat agatgcaacc ataaaaagct ggtatcctct tgtaatcgag atgtcggagg
     2761 acgtgaagga gacggtaacc ggaagatgga aagaatactg gacgaagtag aaaagctgtg
     2821 tttcgacaag gagcttttcc ctataatcaa taaggtcgcc tccggggagc gtttatcctt
     2881 cgaagacggc ctcacggtga tgaacacccc cgacctcaac acggtcggca tgctcgccga
     2941 ttacgtcaag agaaagaggg cgggggacaa ggtctatttc gtcgtcaacc gtcacgtgaa
     3001 ccctaccaac atctgcgcga tatcgtgcaa attctgcgct ttcggtacga ccaagaagtc
     3061 ggctaatgcg tacgagctct cgcacgagca gatactgtcc ctcttgagcg acgacatccg
     3121 cgaggtgcac atagtgggcg gactccaccc cgagtgggag ttccggcact atctagacat
     3181 cgtcaagctc gtcaaggacc actttccgaa gacgcacgta aaggccttca ccgcggtcga
     3241 gatcgactgg ttcacggaga taagcggcat ggggctcgaa gaggtgctcg agacgctgag
     3301 ggagacggga gtggacgcgc tcacgggcgg cggagcggaa atactccacc cggacgtgag
     3361 gaagaagata tgcgcgccca agacggtcgc gacgaggtgg gaggagattc acggcctcgc
     3421 tcatcgtatg ggaatcccta cgaacgccac tatcctctac ggtcatatcg aagaaccgtt
     3481 ccacgtggtc gaccactacg agagattgag aaggatagaa gacgaatcgc ccggcttctt
     3541 cgcgttcata ccggttttgt tccagcccga gaatacgggg ctcaagaaac aggtgaagtt
     3601 cttccccgca tcgtacgatc tcaagataca cgcgctcgcg aggctctacc tc
//
LOCUS       DASSJH010000094         3960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1428500, whole genome shotgun sequence.
ACCESSION   DASSJH010000094 DASSJH010000000
VERSION     DASSJH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428500"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFJ67_02000"
     CDS             <1..809
                     /locus_tag="VFJ67_02000"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HET7289183.1"
                     /translation="KSAKLCAALLFMALALSPLGSLYSPKKAEAGELPAPQLVTDAWM
                     FIAAYKADPETQKALLPPGLEPNPAGHIVINMYTVPTATQTSGFGAYTLTYLTVELKD
                     QDSYVLGSDTTIPGRYFVQYFNSSPSMREYVKKIGIPAGEGMTTTIVKDGKLTAVLEA
                     GGQKLIEATAEVGSDLGNFGGGHLNYFGLITSEKDGKTVNEVVKYPIPWVGGTVEMKN
                     PKIKFTVPADHPLNKIKPLSETPDWAIWTKGSFVYPQYIVVSEFTPDAKN"
     gene            complement(892..2157)
                     /locus_tag="VFJ67_02005"
     CDS             complement(892..2157)
                     /locus_tag="VFJ67_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289184.1"
                     /translation="MSDFSSAQDNCIITQITDTVGGESEVWFLNSSGTLGGFDSNADV
                     NGGNPDGSSEAYLTNTGTFDITQLTDNVGLDSFPFDISAGDNLVALSSEGDPTGDNPD
                     GNREIFLYDRASMTFTQITDTAGEDNIVPVLNSDGSLIAFDSSADVTGGNPDNNREIF
                     LFNADTDTFTQLTDTSGVSSLVPDITSDGATIGFHSDADLTGDNSDVSLEVFLFDVSS
                     MTFTQITDGDNLNSATAALSSDGAIVGLNSDTDPTGGNPDNNREIFLFNTNTMQFTQV
                     TDSADTDNGGPSISSDGSRLVFTSRADLTGDNPDGNREVFLFDANTGITTQITDSVGG
                     TFTDAVISGDGTRILFTSGSDIVGENPDGNIEVYFADCRVVHGDGKNSGGCAFAGEAS
                     PGQAGVNAAVMLLVLFVVVSGIVRKRGRN"
     gene            2828..>3960
                     /locus_tag="VFJ67_02010"
     CDS             2828..>3960
                     /locus_tag="VFJ67_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217964.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET7289185.1"
                     /translation="MRKLKIGIIDFITKSPNHSIWARVMHANFMGIMPQVIAKWCEDA
                     GHEVQYFIFTGLEDLSKDAPRDVDLVFISAFTQSAIQAYALSAKYRGEGVVTALGGPH
                     ARCYPEDAVKYFDYVMGITDEELCREVLEDCRPYRPEGVSLSAKSSPKNLPGVRDRWK
                     FISKALEKTTFLFKVVPMIGSLGCPYTCSFCIDASEPYQPLDFDMLKDDLRFVRTKIK
                     RPLVSWYDPNFGVRFDDYMNAIEEAVPVGSIDFIAESSLSLLSEPHLKRLRQNCFQAV
                     LPGIESWFDMGNKSKTGAKQGMDKVKAVSEHINQILSYIPYVQTNFVVGLDSDNGAES
                     FELTKRFVDLAPAAFPAYPLITAFGKAAPLNLEYQSEDRVLPFP"
BASE COUNT          956 a         1135 c          978 g          891 t
ORIGIN      
        1 gaaaatcggc aaagctctgt gccgccttgc tttttatggc gctcgcatta tcccctctcg
       61 ggagtttata cagtccgaaa aaggccgagg ccggtgagct tcccgcgccc cagctcgtta
      121 cggacgcatg gatgttcatc gccgcttaca aggccgaccc cgagacgcag aaagccctcc
      181 tgccgccggg actcgagccc aaccccgcgg gacacattgt gataaacatg tacacggtcc
      241 cgaccgcgac gcagacctcg ggcttcggag cctacacgct cacctacctc accgtcgagc
      301 tcaaggatca ggactcgtat gtactgggtt ccgacacgac gatccccggc aggtacttcg
      361 ttcaatattt caacagctcg ccctctatgc gcgaatacgt aaagaagatc ggaattccgg
      421 ccggcgaggg gatgaccacg accattgtaa aagacgggaa gctcacggcg gttctcgaag
      481 ccggcggaca gaagctcatc gaagcgacgg ctgaggtagg cagtgatctc ggaaacttcg
      541 gcgggggcca tctcaattac ttcgggctca tcacctccga gaaagacggc aagaccgtga
      601 acgaggtcgt gaagtacccg ataccctggg tcgggggcac agtcgagatg aaaaacccga
      661 agataaaatt cacagttccc gcagaccatc cgctgaacaa gataaagccg ctctcggaaa
      721 cgcccgactg ggctatctgg acgaagggca gcttcgtata cccgcagtat atcgtcgtga
      781 gcgaattcac gcccgacgcg aagaattaga acttgttcca tacctccctg cggcggcctc
      841 gtccgctgcg gggagctgct caccggcata gctctacttc ctgaaccgga gttaattcct
      901 tcctctcttc ctcacaatcc ctgaaaccac aacgaacaga acgagcagca tgacggcggc
      961 attaacccct gcctgcccgg gcgaagcctc tccggcgaac gcgcatccgc cgctgttctt
     1021 gccgtcacca tggaccacgc ggcagtcggc gaaatagacc tctatgttcc cgtccgggtt
     1081 ttccccaacg atatccgagc ccgatgtgaa cagtatcctc gtaccgtctc cgcttattac
     1141 ggcgtccgtg aaggtgccgc ccacactatc cgttatctgc gtggtaatgc ccgtattggc
     1201 gtcaaataga aacacctctc tattcccgtc gggattatct ccggtcagat cggcgcgcga
     1261 agtaaatacg agacggctgc cgtccgagct tatagaaggt ccgccattgt ctgtatcagc
     1321 cgaatccgtt acctgtgtaa actgcattgt attggtattg aacagaaata tctcccggtt
     1381 gttatcgggg tttcctcccg tgggatcggt atcgctattg agcccgacaa tcgcgccgtc
     1441 cgagctcaac gccgcagtgg cgctattcaa attatcgccg tccgtgattt gtgtaaacgt
     1501 catggagctc acgtcaaata gaaatacctc caggcttacg tcggagttat ctccggtcag
     1561 gtccgcgtca gaatgaaagc cgatggttgc cccgtcggaa gttatgtccg gaaccagact
     1621 actcactccc gaggtatcgg tcagctgagt gaatgtatca gtatccgcat tgaacaggaa
     1681 tatttccctg ttattatcag ggtttccgcc tgttacatcc gcggaagagt cgaaagcgat
     1741 cagggagccg tcgctgttca gcacgggaac gatattatct tcacccgctg tatccgtaat
     1801 ctgggtgaat gtcatcgaag ctctgtcgta gaggaatatc tccctgttgc cgtccgggtt
     1861 atccccggtc gggtcccctt cagaggacag ggctacgagg ttgtcccctg cagatatatc
     1921 gaaaggaaaa ctgtccaatc ccacgttgtc ggtcaactgc gttatgtcaa acgtacccgt
     1981 gttggtcaga tacgcttcgg aactgccgtc gggattacct ccgtttacgt cggcattgga
     2041 atcaaaacct cccaatgtcc cgcttgagtt caaaaaccat acttcggact ccccgccgac
     2101 cgtgtccgta atctgcgtaa tgatgcagtt atcctgagcc gaggaaaaat cgctcaaaat
     2161 atcaatggga aaaaagggca aaaaaagtac cgtcgcaatt cctattatgt tactgcctaa
     2221 gcgccccgca atattctgca tataacatct tgatataccc ataacgcccg cctccttttg
     2281 cttcatactt cattaacgat cacatggtgc aaagctttat tttgtatcgc agaatatctc
     2341 cccgtgtcga taataaaata aatttataaa gtttacaatt agattttttg gggtggctca
     2401 cattcttttt cataatatcc caatcgtaca tactaaggta tcgcgatatc ctcaagctac
     2461 cgtcgacccg ctgtttcgat ataccgacac cctgcccctt tattttctgt actacgctgc
     2521 atccgggtca attcagcttc acacacgttg atcattacga aaatgaaata aatccgatta
     2581 atccggccga caaggttcct tggagaagat taaaccgccc gcatccgatg acatcagcaa
     2641 attgattgct ttataaagac ccttcgtgta ttgtataatc catttgaagg ggatagggga
     2701 agctcagttc tgcttcccgc ctcggaaatg ctctcttaca cataaaagta agtagaccct
     2761 tctgaattta ttccattcct gattaatcca gaacggcccc tactcgtgac aattaatcag
     2821 gagactgatg cgaaaactca aaatcggcat aatagacttt ataacgaagt ccccgaacca
     2881 ttccatctgg gcgcgcgtaa tgcacgccaa tttcatgggc ataatgcccc aggtcatcgc
     2941 caagtggtgc gaggacgccg ggcatgaggt ccagtatttc atttttacag ggctcgaaga
     3001 cttgtccaag gacgcgcccc gcgatgtcga tttggtcttc ataagcgcgt tcacccaatc
     3061 agccattcag gcctacgcgc ttagcgctaa atacagaggc gagggagtcg tcacggcgct
     3121 tggaggcccg cacgcgcgct gttaccccga agacgcggtg aaatatttcg actacgtaat
     3181 gggtataacg gacgaggagc tttgcaggga agtgctggaa gactgcaggc cctacaggcc
     3241 cgaaggcgtg agcctgtcgg caaagagctc gcccaaaaac cttcccggag tcagggaccg
     3301 gtggaaattt atctcaaagg ctctcgagaa gaccacgttc ctcttcaagg tcgtcccgat
     3361 gataggtagc ctcggctgcc cctatacgtg cagcttctgc atagacgcga gcgagcctta
     3421 tcagccgctc gacttcgata tgctcaagga cgacctcagg ttcgtacgga cgaagatcaa
     3481 acgcccgctc gtaagctggt acgaccccaa cttcggagtg cgcttcgacg actacatgaa
     3541 cgcgatcgaa gaggcggtgc ccgtggggag catcgacttt atcgcagaga gcagcctgtc
     3601 tctcctctcc gagccacatc tgaagcgcct gcgtcaaaac tgcttccagg cagtccttcc
     3661 cggcatagag tcctggttcg atatgggcaa caaatccaag accggcgcga aacaggggat
     3721 ggataaggta aaggccgtct cagagcacat caatcagata ctgagctaca taccctacgt
     3781 ccagacgaac ttcgtcgtcg ggctcgacag cgataacgga gccgagtcgt tcgagcttac
     3841 gaaaaggttc gtcgatctcg cgcccgccgc gttcccggcc tacccgctca tcacggcgtt
     3901 cggcaaggcc gcgcctctca acctcgaata ccagagcgag gacagggttc tcccgttccc
//
LOCUS       DASSJH010000095         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1102390, whole genome shotgun sequence.
ACCESSION   DASSJH010000095 DASSJH010000000
VERSION     DASSJH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102390"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFJ67_02015"
     CDS             complement(<1..314)
                     /locus_tag="VFJ67_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7289186.1"
                     /translation="MDLSSLDLKDVCNLNGFSGTVPLFPLSTVVFFPNTLLPLHVFEP
                     RYKQMVQDVSASERIIGMALLKPGWESNYYGNPEVFDVVGMGRIVSSETFEDGRINIV
                     LYG"
     gene            501..824
                     /gene="trxA"
                     /locus_tag="VFJ67_02020"
     CDS             501..824
                     /gene="trxA"
                     /locus_tag="VFJ67_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7289187.1"
                     /translation="MGKTVYVNDDNFEEEVLKSELPVLVDFWAEWCGPCRALSPTLEE
                     IAADYEGRVKIAKLNVDENPGQARAFGIRAIPTMIIFKEGSPGERIMGVLPKKHITDV
                     IDGTL"
     gene            1017..1583
                     /locus_tag="VFJ67_02025"
     CDS             1017..1583
                     /locus_tag="VFJ67_02025"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET7289188.1"
                     /translation="MAKKRITRVYTKTGDEGLTSLIGGMRVSKASTRVDAYGDVDELN
                     AVLGLARTAVRNKVLDEILGSVQKDLFIVGADLASPQGLDVPRIKEERIVALERAIDK
                     LLLNLEPLKEFILPGGKPGGSYLHLARTVARRAERKVVKLMEEEGPESGRNVLVYLNR
                     LSDLLFVMARIENKDDEFGETFAEFGRK"
     gene            1580..2230
                     /locus_tag="VFJ67_02030"
     CDS             1580..2230
                     /locus_tag="VFJ67_02030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET7289189.1"
                     /translation="MKRASHSETYIVNPDIDAYLERLTPAGDEILEEMEEYAKRKEFP
                     IVGPLVGRLLFQLAVVSGAKNVIELGSGFGYSAYWFAKAVGGDGGVIYTDTSAENARM
                     AADYFSRAGIMDRLKMITGDALSVLEQSRGGFDVIFNDIDKEYYPLVVDAAYEKLRRG
                     GIFITDNVLWSGRVLTDDDSAGTEGVREFTRRLLAHGGFFTSIIPLRDGLSVSVKL"
     gene            2282..>2631
                     /locus_tag="VFJ67_02035"
     CDS             2282..>2631
                     /locus_tag="VFJ67_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET7289190.1"
                     /translation="MSKAADIVLANGDLVRTLKERISRKNMELMAFHQNKSKRNKALS
                     GSMLAKKRSEIVDKLIKQALSELGFNDLKNISVVALGGYGRNELCPYSDIDIMFLYKP
                     RNKTLAKEATEKLL"
BASE COUNT          669 a          636 c          770 g          556 t
ORIGIN      
        1 ccgtagagga ctatatttat tctgccgtcc tcgaaggtct ccgagctcac tatcctaccc
       61 atcccgacga cgtcgaacac ttccgggttg ccgtagtagt tcgactccca accgggtttc
      121 aggagcgcca tgcctattat ccgttcggag gcgctcacgt cctgaaccat ctgtttatac
      181 ctcggctcga acacgtggag agggaggagc gtattcggaa agaacacgac cgtagataac
      241 gggaaaagcg gtacggtccc ggaaaaaccg ttgagattgc atacgtcttt cagatccaga
      301 gaagacaggt ccataataaa tcaatccaga ctattggtta agactaattt tagtatagca
      361 tattcggcgt tttatgttgt gtttgtgatc acatccggcc gtgtgacgag cggggagcgc
      421 ggcgggctca agcttgaatc ctttaccggt gatgaatatc taatatcttg tgaagtttcg
      481 ttttccacgg gaggtccgaa atgggcaaaa cagtatatgt caacgatgac aatttcgagg
      541 aagaggtgct taaatccgag ctgccggtgc tggtagattt ctgggccgaa tggtgcggcc
      601 cttgccgcgc cctgtcgcct acacttgagg agatcgctgc cgattacgag gggcgtgtga
      661 agattgccaa gctcaacgtc gacgagaatc ccggacaggc gagagccttc ggtataaggg
      721 ctatacctac aatgataata ttcaaagaag ggagtccggg ggaaaggatc atgggcgtgc
      781 ttcccaagaa acacatcacg gacgtgatcg acgggacgct ttagacagct ccggccgagg
      841 cgattaaccg attattcgcc ggccttaaat tgatgccgcc ggcgtcgcgg ccggcgcccg
      901 tccgagcgcg ctgattcaat cggcatcgcc gcagtatttc caatctctcc gggatccgaa
      961 aaacaatgca aatccatagg tttgaaccat actgaccgtt cggttagaat ttcaacgtgg
     1021 cgaaaaagag gattaccagg gtatacacga agacagggga cgagggcctt acttcgctca
     1081 taggcggtat gagggtgagc aaggcttcca cccgtgtgga tgcctatgga gacgtcgacg
     1141 agttaaacgc ggtgctgggg cttgcccgca ccgcagtgcg taacaaagta ctcgacgaaa
     1201 ttctcggatc ggtacagaag gacctcttca tcgtaggggc cgatctcgcg agcccacagg
     1261 gtctcgacgt tccgaggata aaggaagaaa ggatagtcgc gcttgagcgc gccatagaca
     1321 agcttcttct gaatctcgag ccgcttaagg aattcatact accgggcgga aagcccggcg
     1381 gatcgtatct ccatctcgcg aggacggtgg cgaggagagc ggagagaaag gtcgtgaagc
     1441 tgatggagga agagggccct gagtcgggac gtaacgtgct cgtctatctt aacaggctct
     1501 ccgacctgct gttcgtgatg gcgagaatag agaacaagga cgacgagttc ggggaaacat
     1561 tcgcggagtt cggcagaaaa tgaagcgggc ttcgcattcg gaaacataca tagtcaatcc
     1621 tgatatcgat gcatacctgg agcgtctcac gcccgcagga gacgaaatac tggaagagat
     1681 ggaggaatac gcgaagagga aggaattccc catcgtcggg cctcttgtcg ggagactcct
     1741 cttccagctc gcggtcgtaa gcggcgcgaa aaacgtgatc gagctcggct cgggcttcgg
     1801 ctactcggct tattggttcg cgaaagccgt gggcggggac ggaggggtca tatataccga
     1861 cacgtcggcc gagaacgccc gcatggctgc tgactacttc agcagagcgg gtatcatgga
     1921 caggctgaaa atgataacgg gagacgcgct ctcggtgctc gaacaatcgc ggggagggtt
     1981 cgacgtcata ttcaacgaca ttgataagga gtattatccg cttgtcgtcg acgcagctta
     2041 tgaaaaatta cggaggggcg gtatattcat taccgacaac gtgctgtggt cgggaagggt
     2101 gctcactgat gacgactcgg ccggcacgga aggcgtgagg gagttcaccc gtcgactcct
     2161 ggcgcacggg ggatttttca cttccattat tccgctaagg gacgggcttt cggtcagcgt
     2221 gaagctataa taatgctaaa atatattgat tcagatgtaa tataggagtt tagagaacag
     2281 tatgagcaaa gccgccgata tcgttctagc aaatggtgac ctggtcagga ccctcaaaga
     2341 aaggatcagc aggaagaaca tggagcttat ggcgtttcat cagaataagt cgaagcgcaa
     2401 taaggccctc tccgggagca tgctcgcaaa aaagcgctcc gagatcgtgg acaagctgat
     2461 aaagcaggcc ctctccgagc ttggatttaa cgacctcaag aatatcagcg tcgtggctct
     2521 gggcggctac gggaggaacg agctctgtcc ctactccgac atcgatataa tgtttctcta
     2581 caagccgcgc aacaaaacac tggcgaagga agcgacggag aagctccttt a
//
LOCUS       DASSJH010000096         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_994507, whole genome shotgun sequence.
ACCESSION   DASSJH010000096 DASSJH010000000
VERSION     DASSJH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994507"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFJ67_02040"
     CDS             <1..389
                     /locus_tag="VFJ67_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7289191.1"
                     /translation="ISWAASNIWVKKRMAGEDIYRISALQLIFGTLGLLIIALPTHGV
                     FSIEWTWNSVYVLLFTGLIASAVDFTIWFYLIKKLDINITTFSSMLVPVFGLFFDWLI
                     LGSGLDWGTVGGGALIIAGIYMVSKR"
     gene            542..>3446
                     /locus_tag="VFJ67_02045"
     CDS             542..>3446
                     /locus_tag="VFJ67_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289192.1"
                     /translation="MRHKILIAAAIVIVIFISVTIAAYFFTQTEHFRTLVKNTAERLV
                     SSSTGQTFRIGSVEGNFFYNIKLKDVRFEVENESFVSVDELSVTYSIPRMLDTAVLTS
                     KVIPIDGITLSGARVNLIKYGDGTWNFGKIGSKSAKEKEKDKEKKGPLEWSIIASGLD
                     INDSSLKIDDRESGKVSEYRIDDTELSAKLINITDEVQVDIKNADFEAPSQGLTVKGL
                     KVNAVYTGEKAKISGLEVTLNGAGIKLDAEAGGLRDNPEISYALSVNGYKVEDVGTFS
                     IESEGKGVFRGPKNIDAEINIKIPESELFGKKLSGTLDKITISGTAIDLGEGNIQSEL
                     GGLSLTGKGDLARLISGEGKNSFSVRASLKDVKTTEIFSLIEEKTEVVNTKLGAVLNA
                     DLIADGSWAEFRDMNVNGKIERLDVQGKKAGDLKLTGTASYSPSGVGVDVKTVLKQVD
                     LGTILSRENLHGKINSDLAIKGLIPLEGDVLQKMSAAVKGKVGPSSVFNVNIKKGDID
                     ASYEKERLTVRALAIDGDSFDLRVKGGAAARKGVNLDYDIDVKDLGLVSKFAPGADLA
                     GKLKASGKVEGEIKNPHITIDAEAEDLGFNDLFSAQSLKITGDGSIDLNNPDLRAKIS
                     TGNARIKDRDIESMEIDAESKGKGIDVTALIRENDKSEYEVAALLTDLGASEKHIEIS
                     KLRLDMDETELENRDRILLTVGPGKLIVDSFNLYYKDSSALADARIFYDGSVEGRLNL
                     KNLSLNDIVQVINPKADVNGTLSADADVSGTMSNPRFSVKAETKDLSYKDFKDDVSLD
                     LGYLDRNLNMKFLVTGDSGVVLQAAGNAEMALDLNNMSKNMNDTRFNLTVRSDGVDLS
                     PLTSLSSEIEKSSGLLVVDVKAGGTFGSPRAEGQITLKDGVFKIKSLQNELKIVNALI
                     ELDGQKGYLRQAEIDSGKGKGTFEGQIDVSSLAYDLNGSMKNFLLRPKRITAEI"
BASE COUNT          969 a          848 c          971 g          658 t
ORIGIN      
        1 ctatttcctg ggccgcgtcg aatatctggg tcaaaaaaag gatggccgga gaggacatat
       61 acaggataag cgcgctccag ctcattttcg gaacgctcgg tcttctcatt atcgcgctgc
      121 ccacacacgg agtgttcagc atcgagtgga catggaactc cgtttacgtg ctgttgttta
      181 ccgggctcat cgcctcggca gtcgacttca cgatatggtt ctacctgata aagaagctcg
      241 acatcaacat cacgacattc tcctcgatgc tcgtcccggt attcggcctg ttcttcgatt
      301 ggctcattct cgggagcggg ctcgactggg ggacagtcgg gggaggggcg ctcatcattg
      361 cgggaattta catggtttcc aagagataag ccgcctcgac cggccactta ttcaggttca
      421 atttgcagaa tcaataatgt ataattgtgg caaagcccgg ttgggcgaat gttgaatgac
      481 cggtccatga tgacaggaac cgaccgaatc cgtgaactcg cgctccccga aaagtcatta
      541 tatgcgtcat aagatactca tagccgcagc catagtgatc gtcatcttta tatccgttac
      601 gatagcagcc tattttttta cccagaccga gcatttcagg accctggtta aaaataccgc
      661 cgagaggctc gtcagctcat cgacaggcca gacattcagg ataggttcgg tcgagggcaa
      721 cttcttttac aacataaagc tcaaggatgt aagattcgaa gtcgaaaacg aaagcttcgt
      781 ctcggtggac gagctctcgg ttacgtactc gatcccgcgg atgctggaca cggccgtgct
      841 aacaagcaaa gtgatcccca tcgacgggat aacgttaagc ggggcgagag tgaacctcat
      901 caaatacggc gacggcacgt ggaacttcgg caagatcggg agcaagagcg caaaggagaa
      961 ggaaaaggac aaagagaaga aaggaccgct cgaatggagc attatagcct ccggtctcga
     1021 tattaacgac agctcgctca agatcgacga cagggagagc gggaaggttt ccgagtaccg
     1081 gatcgacgat acggagctat ccgcgaagct catcaatatc acggatgaag tacaagttga
     1141 catcaaaaac gccgacttcg aggcgccgtc acagggattg accgtcaagg gattaaaagt
     1201 taatgccgtc tataccgggg agaaggcaaa gatatcgggc ctcgaggtaa ctctcaacgg
     1261 ggccgggatc aagctcgacg ccgaagcggg cggtctcaga gacaaccccg aaatctccta
     1321 tgcgctctcg gtaaacggtt ataaggtcga agacgtaggc actttcagca tcgagtcgga
     1381 aggaaaaggc gtattcaggg gacctaaaaa tatcgacgcc gaaattaaca taaaaatccc
     1441 ggaatcggag ctcttcggga aaaaactttc cgggactctc gataaaataa ctatctcggg
     1501 aacggccatc gacttaggcg aggggaacat acaatccgag ctcggcgggc tgtcgcttac
     1561 agggaaggga gacctcgcgc gtcttatttc cggcgagggg aaaaacagct tcagcgtcag
     1621 agcctcgctc aaggacgtga agacgaccga gatattctcg ctcatcgaag agaaaacaga
     1681 ggtggtcaat acaaagcttg gcgcggtact taatgccgac ctcatcgcgg acgggagctg
     1741 ggccgaattc agggatatga acgtcaacgg caagatagag cgtctcgatg tgcaggggaa
     1801 aaaggcagga gacctgaaac tgaccggcac ggcttcctac tcgccctcgg gcgtcggggt
     1861 ggacgtgaag accgttctca agcaggtcga cctcggcacg atactcagca gggagaacct
     1921 gcacggcaaa ataaattccg atctcgccat caagggactg ataccgctcg aaggggacgt
     1981 gcttcaaaag atgagcgctg cggttaaagg aaaggtcggg ccgtcgagcg tattcaacgt
     2041 caatatcaaa aagggcgata tagacgcgtc atacgagaaa gagcgtctga ctgtgagagc
     2101 gcttgcgatc gacggggatt cattcgatct cagggtcaag ggcggggccg ccgccaggaa
     2161 aggggtcaac ctcgattacg atatcgacgt aaaagacctc ggcctcgtat cgaaattcgc
     2221 cccaggggcc gatctcgcag gcaagctaaa ggcgtcgggg aaagttgaag gtgaaataaa
     2281 aaaccctcac ataactatag acgccgaggc ggaggatcta gggtttaacg atctattctc
     2341 cgcacagtct ctcaaaataa cgggggacgg ctcgatagac ctcaataatc ccgacctcag
     2401 ggcgaagatc agcaccggga acgccaggat caaggacaga gatatagaga gcatggaaat
     2461 cgacgcagag agtaagggaa aaggaataga cgtaaccgcg ctgataaggg agaacgacaa
     2521 atccgaatat gaagtcgcgg cattgctcac agacctcggc gcaagcgaga agcacataga
     2581 aatcagcaag ctcagactag atatggacga gaccgagctc gagaacaggg acaggatcct
     2641 cctcaccgta gggcccggca agctcatagt ggattctttt aacctctatt ataaagattc
     2701 atcggcgctc gcagacgccc gcatcttcta cgacggtagc gtcgaagggc ggctaaatct
     2761 gaagaacctg agcctgaacg acatcgttca ggtcattaat ccgaaagctg acgtcaatgg
     2821 gactttatcc gctgacgccg acgtgagcgg taccatgtcg aatccgcggt tcagcgtgaa
     2881 ggcggaaacg aaggacctct cgtacaagga ttttaaagac gatgtatcgc tcgacctcgg
     2941 ctacctcgac aggaacctca atatgaaatt cctcgtgacc ggagactcag gcgtcgtgct
     3001 gcaggccgcg gggaacgcag aaatggcgct tgacctcaac aacatgagta aaaatatgaa
     3061 cgacacgagg ttcaacctca cagtccgctc ggacggggtg gacctgagcc cgctgacgag
     3121 cctgagcagc gagatcgaaa aatcatcggg tctgctggta gtggacgtga aggccggggg
     3181 cactttcggc agtcccagag cagagggtca gataactctc aaggacgggg tgtttaagat
     3241 caaatctctt cagaacgagt taaagatcgt gaacgccctc atcgaactcg acggtcaaaa
     3301 aggctatctg agacaggcag agatcgattc cggcaagggg aaaggcacgt tcgaagggca
     3361 gatcgacgtt tcgtcacttg catacgatct caacggaagc atgaagaatt tccttttaag
     3421 accgaaacgg ataacggccg agataa
//
LOCUS       DASSJH010000097         3860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_23082, whole genome shotgun sequence.
ACCESSION   DASSJH010000097 DASSJH010000000
VERSION     DASSJH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23082"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..682)
                     /gene="msrA"
                     /locus_tag="VFJ67_02050"
     CDS             complement(53..682)
                     /gene="msrA"
                     /locus_tag="VFJ67_02050"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7289193.1"
                     /translation="MKRIMFLAAMLILLLAGASTRAGENSSTQDAGYETATFAGGCFW
                     CIQPPFDKLDGVVSTTVGFTGGKEKNPTYEQVSSGTTGHAESIEIVYDPKKVTYDELL
                     QVFWMNINPTDSGGQFVDRGKQYRPAIFYHNEAQKKAAEASKKKLQESGRFDKPIVVE
                     IVEATEFYPAEDYHQKFYQKSPVRYKTYRWGSGRDQFIEEYWGAAALSH"
     gene            complement(827..2200)
                     /locus_tag="VFJ67_02055"
     CDS             complement(827..2200)
                     /locus_tag="VFJ67_02055"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7289194.1"
                     /translation="MSSLILIIEDERLLCKQLHKALTQEGYSVISSYEGGEGINLARR
                     EDPDLVLLDLKLPDTDGLEVLKAFSRWERPPTTIMMTAHGNVEVAVTAIRVGAYDFIE
                     KPFPLDKLKVMVRNALKTSELKNSLSAAAMRAQEKYGFGSLIGTSPVIKDLQSLFRKL
                     TETDPRTILITGESGTGKGLAAKILHYNGVRKQGPFIEINCAAIPETLLESELFGHEA
                     GAFTDAKKMKKGILEQADGGTVFLDEIGDMSLSLQAKLVKAVEERSFRRLGGNRDISV
                     DLSVIAATNHDLKSLVKHSEFREDLYHRLRVISFEMPALRDRKEDIPVLIDHFISYFN
                     SDLSKNITEIPDEIRKTFMNYNWPGNVRELRSTIERAVLLSEDGVLNPKYIMLEEGET
                     LKVQNSDEKMVIDVPIEEASLYKIEKKVITKALDLNNWNQTRTAEMLGITREVLRYRM
                     KKWGLLS"
     gene            complement(2231..3514)
                     /locus_tag="VFJ67_02060"
     CDS             complement(2231..3514)
                     /locus_tag="VFJ67_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7289195.1"
                     /translation="MFFFMLLISGLSIYSIYNLDKTASNIRGRYNTLSKLLSEKEEGK
                     QGIFANEMLLEAIRISDKQIKYSYVMILTVVGVTMLFGIVLTIFIPRMITKPIQSLFN
                     AAESVAGGDYSVRLKDVKTSSEITTLVRAFNNMIDNIEKKSGELQKKNEEIMKLLETT
                     RRFNELLEYEIAQATREVEEKHWELMKAEKLAAIGELATGVAHEVRNPLSGIGLALEL
                     MKDETENEEHKQTIRDILNEISRLERIVKGLFQLGHPKSIQLIECTPNDIVERALNLV
                     SMKAKEKGITIKKDLACAGSFYVDHEQIEQVILNLLINGIEATGGSGEVRVETRSNNG
                     SVEISVSDTGCGVPDEEMDKILQPFYSTKERGTGLGLAISSRIVEAHKGKLHITSRVG
                     EGSTFVVEIPSDLNVELGTSDQTEAAQGADNYKLN"
BASE COUNT          706 a         1127 c          974 g         1053 t
ORIGIN      
        1 tcttcaacga aaatattata ctcggcgagg aggcgataat acagataatt cgtcaatggc
       61 tgagtgcggc cgcgccccag tattcctcta tgaactggtc ccttcccgag ccccacctgt
      121 aggttttata cctgacaggg cttttttgat agaacttctg gtgatagtcc tcggccgggt
      181 agaattcggt cgcctcgact atctcgacga cgatgggctt atcgaacctg cccgactcct
      241 ggagtttctt tttcgaggcc tcggccgctt ttttctgcgc ttcgttgtgg tagaagatcg
      301 cgggcctgta ctgcttcccc ctgtcgacga actgtccgcc cgagtcggtc gggtttatgt
      361 tcatccagaa tacctgcagg agctcgtcgt aagtgacctt cttcgggtcg taaacgatct
      421 cgatcgattc cgcgtgtccg gtcgtcccgg aagagacctg ctcgtaggtc gggttcttct
      481 ccttgccgcc cgtgaaaccc acagtcgtgc tcacgacgcc gtccagcttg tcgaacggtg
      541 gctggataca ccagaaacac cctcccgcga acgtggcggt ctcgtagccc gcatcctgcg
      601 tggagctatt ctccccggcg cgtgtagacg cgcccgctaa caataagatc agcatcgctg
      661 caaggaacat aattcttttc atctgttttt cccctcggga ttcaaggata aaggaacccc
      721 ttattgagct tatgacatat atacggtttt actattcaaa aggattcaag atagttgatc
      781 cggccgagcc ctaggaaatc cgcggtgacg ggagcggagc gttacgtcag ctgaggagtc
      841 cccacttctt catgcggtac ctgagcacct cgcgcgtgat gccgagcatc tcggcggtcc
      901 tggtctggtt ccagttgtta aggtcgagcg ccttggttat cacctttttt tctatcttgt
      961 agagggacgc ttcttctatc gggacgtcta tcaccatctt ttcgtccgag ttctgcactt
     1021 ttaaggtctc gccctcttcg agcattatgt atttcgggtt gagcacgccg tcctcgctca
     1081 gcagcaccgc cctttctatg gtgctcctga gctccctcac gttccccggc cagttgtaat
     1141 tcatgaacgt ctttctgatc tcatcgggaa tttcggtgat gttcttgctg aggtcagaat
     1201 tgaaatacga gatgaaatgg tcgatgagca cggggatgtc ctctttcctg tcccggagag
     1261 cgggcatctc gaagcttatc accctgagcc tgtgatagag gtcttccctg aactcgctgt
     1321 gttttactag agacttgagg tcgtggttgg tggctgcaat gaccgagagg tcgaccgaga
     1381 tgtccctgtt gccgcctaac ctcctgaaag agcgctcctc gaccgccttt acgagctttg
     1441 cctggagcga gaggctcatg tcgcctatct cgtcgaggaa cacggtccct ccgtccgcct
     1501 gctcgaggat ccccttcttc atcttcttcg cgtccgtgaa agccccggcc tcgtgcccga
     1561 agagctcgct ttcgaggagc gtttccggta tagccgcgca gttgatctct atgaacgggc
     1621 cctgcttcct tacgccgtta taatggagta tcttcgccgc gagccccttc ccggtcccgc
     1681 tttcaccggt gatgagtata gtcctggggt cggtctcagt gagcttcctg aagagcgatt
     1741 gaaggtcttt tatcacgggg ctcgtgccta tgagtgaccc gaacccgtat ttctcctgcg
     1801 ccctcatggc agccgcgctc aggctgttct tgagctcgct agtcttgagg gcgttcctta
     1861 ccatgacctt gagcttatcg agcgggaacg gcttctctat gaagtcgtac gcccccaccc
     1921 ttatggccgt gaccgcgacc tccacgttcc cgtgagcggt catcattatt gtagtcgggg
     1981 gacgctccca ccttgagaac gccttcagca cctcgagccc gtccgtgtcg gggagcttga
     2041 ggtcgagaag gacgaggtcc gggtcctccc tcctcgcgag gttgatccct tcaccacctt
     2101 cgtaggagct tatgacggaa tagccctcct gcgttagagc cttatgcagc tgcttgcaga
     2161 ggagcctctc gtcttctatg ataagtatca gtgagctcat ttttatattg agttctccgc
     2221 tcccttcgga ttaattaagt ttatagttgt ccgctccctg agcggcctca gtttgatcag
     2281 aggtcccgag ctcgacgtta aggtcgctcg ggatctcgac cacgaacgtc gagccctcgc
     2341 cgaccctgct cgtgatgtgg agcttaccct tgtgagcctc cacgattctg ctcgaaatag
     2401 caagcccgag ccccgtcccc cgctccttcg tcgaatagaa cggctggagt atcttatcca
     2461 tctcctcgtc aggcactccg cagcccgtgt cggatacgga tatttcgacg gagccgttat
     2521 tgctcctcgt ctcgacccgg acttcgccgc tgccgcctgt agcctctatg ccgtttatga
     2581 gcaggttaag tatgacctgc tctatctgtt catggtccac gtagaagctg cccgcgcagg
     2641 cgaggtcttt tttgatcgtt atccctttct ccttcgcttt catgctcaca aggttgagcg
     2701 ccctctcgac tatatcgttg ggcgtgcact cgatgagctg tatgctcttc ggatgcccga
     2761 gctggaatag tcccttgact attcgctcga gcctcgatat ctcgttgagt atgtccctta
     2821 tggtctgctt gtgctcctcg ttctctgtct cgtccttcat cagctcgagc gcgagcccta
     2881 tccccgaaag cgggttcctc acctcgtgcg ccacgcccgt cgcgagctcc cctatcgccg
     2941 ccagcttctc cgctttcatg agctcccagt gcttctcctc gacctcgcgc gtagcctgag
     3001 cgatctcata ttcgaggagc tcgttgaacc tcctggtcgt ctctagcagc ttcatgatct
     3061 cctcgttctt cttctgtagc tccccgctct tcttttctat gttgtctatc atgttgttaa
     3121 acgccctgac gagcgtagtt atctcgctcg acgtcttcac gtccttgagc cttacggaat
     3181 aatcccctcc cgccaccgat tcggcggcgt tgaagaggct ctgaatgggt ttggttatca
     3241 tcctcggtat gaatatcgtg agcactatac cgaagagcat cgtaactccg acgacggtca
     3301 atatcattac gtacgagtac tttatctgct tgtcggagat ccttatggct tcgagcagca
     3361 tctcgttagc gaatatgccc tgcttcccct cctccttttc gctgaggagc ttggagagcg
     3421 tgttatacct gcccctgatg ttggatgccg tcttgtcgag gttgtagatg ctgtagatcg
     3481 acagcccgct tatgagcagc ataaagaaaa acatcaaggc atatccgagg gttatctgtt
     3541 tactgaggtt caaatggcac gcaccgatct ttatacgtta atgcatctgt taaaaattct
     3601 acctgttatt gaggtcatct cataccgaat ttttctccgg ccggcggcgg tttaggccgg
     3661 actcccgggg aggaatcagt ctgcgggtct cagccgcagg gttcaattta tcattaatta
     3721 agattacctg ataacgacgc ttatatcccg gccccggcct ggagggaagg cccgggggcc
     3781 ctttaagaat tcagcccccg tacgaccctg taaacggata atctcatgaa catccatata
     3841 gataaatccg cagaatcggg
//
LOCUS       DASSJH010000098         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_886965, whole genome shotgun sequence.
ACCESSION   DASSJH010000098 DASSJH010000000
VERSION     DASSJH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886965"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1364)
                     /locus_tag="VFJ67_02065"
     CDS             complement(195..1364)
                     /locus_tag="VFJ67_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7289196.1"
                     /translation="MINTERMTEHVMDLIRIDSPSKREKEVALRLEKDMKELGAECFY
                     DNAGEKVNGNVGNLIVRLKGNKSGVAPLLLSAHMDTVGPGEGIRPRIENGLIKSDGTT
                     ILGSDDKSGVAVIVEVLRTLRERDIPHGDIEVAFTICEEIGLLGAKYIDISKFDSRYG
                     IVLDSSTPSRLVLRCPSAVKMEFTVHGLEAHAGVCPENGISAIKIASEAIARMKLGRI
                     DEITTANIGIIKGGRATNIIPNLVKAVGEARSHSEESLKAQLDHMRMCFHDAVSEYEV
                     TIRDDLAVEGTTRIATLEESIERSYDRMDVPPDSAPARLVETAVANLGHTIERHTSGG
                     GCDANYFNKMGIECVNLGTGMYDLHTVNEYLVLEEFYRCSEIVLESVKLNALSAR"
     gene            complement(1579..1836)
                     /locus_tag="VFJ67_02070"
     CDS             complement(1579..1836)
                     /locus_tag="VFJ67_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7289197.1"
                     /translation="MGCAFKAISDERRRTILKILGKKDASAGEIASKFNISQPTVSNH
                     LKILKEAGLINEQKIKQNRIYSLNRAELKKVISEIEELITG"
     gene            complement(2197..2634)
                     /locus_tag="VFJ67_02075"
     CDS             complement(2197..2634)
                     /locus_tag="VFJ67_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7289198.1"
                     /translation="MVGSIFRTSDDIGAFIARITLGVVMLPHGLQKLLGLFGGAGFSG
                     TVEFFVGSGLPAFIAVLIIISESFGSLGLIFGFLSRLAALGVIIIMLGAIVTVHIKNG
                     FFMNWSGSGPGEGFEFHLLAIGLGLVVLFKGGGAWSVDRVISG"
BASE COUNT          694 a          835 c          796 g          863 t
ORIGIN      
        1 cgaatgcctg cgcgacggtg ggctcggcca gctccatgtg gcaatacttt acgatgtcga
       61 acgggttttt cccgctcgac tctatgagcc cgactatttt tgcgagcgtg tcgttcgctt
      121 cttccacggc gcttccgtgg tcgacgatta taagggcttt cttgctcata atttcacgaa
      181 gagggcaggg gaattcagcg ggcgctcagg gcgttgagct tcacggattc gagcactatc
      241 tccgagcagc ggtagaattc ctcgagcacc aggtactcgt tcacggtgtg gaggtcgtac
      301 ataccggtgc cgaggttcac gcattcgatc cccatcttgt tgaagtagtt ggcgtcgcat
      361 ccgcctccgc tcgtgtggcg ctcgatcgta tgtcccagat tcgcgaccgc cgtctccacg
      421 agccgggccg gcgcggagtc gggcgggacg tccatcctgt cgtagctcct ctcgatcgat
      481 tcctcgagcg tcgcgatacg cgtcgtaccc tctacggcga ggtcgtcgcg gatcgtgact
      541 tcgtattctg aaacggcgtc gtggaagcac atacgcatgt gatcgagctg cgcctttagt
      601 gactcttcgc tgtgactcct cgcttctccc accgccttta caagattggg aatgatattc
      661 gtcgccctgc ctcccttgat tatccctatg ttggcggtcg taatctcgtc tatcctgccg
      721 agcttcatcc gtgcgatagc ttcgctcgcg atctttatag cgcttattcc gttctccggg
      781 cacacgcccg cgtgggcttc gaggccatga accgtgaatt ccatcttcac cgccgacggg
      841 catctcagga cgagccgtga gggcgtgctg ctgtcgagca ctattccgta cctggaatcg
      901 aatttcgaga tgtctatgta cttggcaccg aggagcccga tctcctcgca tatggtgaac
      961 gccacctcga tgtcgccgtg cggaatgtcg cgctccctga gagtcctcag gacctctacg
     1021 atcaccgcga cgccgctttt atcatcgctg cccagtatcg tcgtcccgtc gctctttatg
     1081 agtccgtttt ctatcctggg cctgatgcct tcgccgggtc cgaccgtgtc catatgcgcc
     1141 gacagtaaaa gaggggcgac cccggattta ttgcccttta gcctgactat gagattcccg
     1201 acgtttccgt ttaccttttc tcccgcattg tcgtagaagc attcggctcc gagctccttc
     1261 atatctttct ccagcctgag tgcgacttcc ttttccctct tagatgggct gtcgatcctg
     1321 atgaggtcca tgacgtgctc ggtcattctt tccgtattaa tcataaatct ccccgtccgg
     1381 cattcacaac gcggattgag tatgttacga gattttttcc aaacccggac tcatccagta
     1441 taactcaagc ggagtatttt aggaatcggg gcgtgtccgg tttccgaagc acgggaatgc
     1501 atcagaatgt aaaggagcgg tcagaacgaa aaagcccggt cgcttttaaa tcatattgtc
     1561 gatccgcgat aagggccgtt atccggtaat caattcttcg atctctgata ttaccttctt
     1621 gagctcggcc ctgttcagtg aatagatgcg gttctgcttt attttctgct cgttgatcaa
     1681 ccccgcttcc ttcaggatct tcagatggtt tgagacagtc ggctgcgaga tattgaactt
     1741 gctcgcgatt tcgccggcgc tagcgtcttt tttccctaat attttaagaa tcgtccttct
     1801 gcgttcgtca cttatggctt tgaaagcaca gcccatatca tcccccgcca ggttcgtaat
     1861 tattaaatta tgaaattcat tttatatgat gaggtttgta aaatcaactg tgcacaagat
     1921 aattctttta acggtttcgg agaggccgtt tcgaaaaggc catgaataca gggtttctac
     1981 gatccggggg tgaatcttat tatttccggc cgaagaggga tgaatagctc attaggaacc
     2041 cgctttacta aatcaatatc agcggaaggg gcgagcttca caatgcggga cggatgccgg
     2101 cggggcaaac gaaaatacgc gcgtactctc ggaccgaagt gtaatagtct ggcatcgcag
     2161 cgcaatgggg attggatatt tgcgggcaaa gccgcatcat ccggatatga cgcggtcgac
     2221 ggaccatgcg ccgccgccct tgaagagaac gactaatccg agccctatag ccagtaggtg
     2281 gaattcgaag ccttcaccgg gtccgctgcc gctccagttc atgaagaagc cgttctttat
     2341 gtgtactgtg actatcgccc cgagcatgat tattatgacc ccgagagccg cgagcctgct
     2401 cagaaagccg aatataaggc cgagcgagcc gaaggattcg ctgataatta tgaggacggc
     2461 tatgaacgcc ggtaagccgc tgcccacaaa aaattcgacc gtgcccgaga accctgcccc
     2521 accgaatagc cccagcagtt tctgaagccc gtggggcagc atgactaccc cgagagtgat
     2581 cctcgcaatg aacgcgccta tatcgtcgct tgtgcggaat atcgagccca ccatgatgca
     2641 ccccctttga ttttcatgcg cctgcaatca tattttatct tataggcgcg atcgaattca
     2701 agaccgcggt ttcttcgctc acactgtgca ccgcgcttga gtcgtttcag gggggggtat
     2761 tcatcgggac tcgtataggg accgtaaatt ttcggagtgt gcggccgata tatgcaatat
     2821 atgaaaatgc ttattatgat cgcatatata aaatatttat aaccgggcgg atggcccggc
     2881 ctggttcgga tggccggcta tttatggaat cctgatccct tccattaatt tctcgtttaa
     2941 gcatcgtaaa gtattacttt aaggtaaagt tgagtctaat ataatggatt atatattacg
     3001 tgcaacaaac tcatattact gtataatata acttggtata ctagtttaac tatttttttg
     3061 cgcctttagg tgctgaattt tatagattta acttttaaag gttgaagaga gaaatcccga
     3121 ctaagctgcc ggaatcatgg taaaagagcc tcctaatgta aattgtcagt ttttaaggaa
     3181 aacttaaa
//
LOCUS       DASSJH010000099         5099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_887021, whole genome shotgun sequence.
ACCESSION   DASSJH010000099 DASSJH010000000
VERSION     DASSJH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887021"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFJ67_02080"
     CDS             complement(<1..342)
                     /locus_tag="VFJ67_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289199.1"
                     /translation="MKPVKGTKKSSKRISLGRDLDRPKWKITIVGTFVLVLFTAVCIK
                     ALDLQVLDRDRAFTIARKQHQGSSTLLPRRGRILDRNMKELAVNVEIKSVFANPYAVK
                     NPAEVSKVLAAN"
     gene            complement(339..677)
                     /locus_tag="VFJ67_02085"
     CDS             complement(339..677)
                     /locus_tag="VFJ67_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289200.1"
                     /translation="MAHVKALGSREKEKTERVGISTGFVILVLIALALALFYVRFKVE
                     ELRIGYEISRNNKQTGELLKEKRILQSEFMRAKSPDSLEKTALEMGFKYPTQEDIIYI
                     EQTTLADGKK"
     gene            complement(680..1639)
                     /gene="rsmH"
                     /locus_tag="VFJ67_02090"
     CDS             complement(680..1639)
                     /gene="rsmH"
                     /locus_tag="VFJ67_02090"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011949033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET7289201.1"
                     /translation="METAVVEISKPIHQSVLSDEAISYLITRPEGVYVDATLGMGGHT
                     KSILDSTHSKSLVIGLDVDEEAISISRERLSAYNGHVIYRNSNFSDMDKVLDGLDIRE
                     VDGILADLGMSSYQVDSSERGFSFMREEPLDMRMDPRLRFTAYDLVNEMTMEEISRVL
                     KMYGEEKWSRRIAKRIVEARAESPIKTSAELARIVSLAIPKKFHPARIHPATKTFQAL
                     RIAVNNELENIEEFIGKAVSRLKPGGRLVIISFHSLEDRLVKTSFLRLSSPCVCPPAM
                     PECGCGKKKELRIITRTPIIPSEEEIQNNPRARSAKMRVGEKV"
     gene            complement(1644..2090)
                     /gene="mraZ"
                     /locus_tag="VFJ67_02095"
     CDS             complement(1644..2090)
                     /gene="mraZ"
                     /locus_tag="VFJ67_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET7289202.1"
                     /translation="MFRGRYEHTMTDKGRVSIPARFREVCREKYADETLIITNFDKCL
                     VAYPLKEWNDIETKVSSLPQFKQEVISFLRYLMGGAVDCPLDGQGRVLIPQPLRNHAR
                     IDKEVIMIGMLTRIEIWAKEVWEQEEQDRAYEEFKKSREILAGQGL"
     gene            2490..2735
                     /locus_tag="VFJ67_02100"
     CDS             2490..2735
                     /locus_tag="VFJ67_02100"
                     /inference="COORDINATES: protein motif:HMM:NF027581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HET7289203.1"
                     /translation="MPTISIKLRNNSESVYFQSIMGTYAHVAWVRTDDPESGIIQVIT
                     TPDLLEETRNILERLRKEIEFEETGPNGETPPPVRRS"
     gene            2738..4315
                     /gene="murJ"
                     /locus_tag="VFJ67_02105"
     CDS             2738..4315
                     /gene="murJ"
                     /locus_tag="VFJ67_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET7289204.1"
                     /translation="MGEKEHIARSVGIVGFITVISRIAGYLRDMQMAYFFGATNFTDA
                     YWIAFRIPNLLRRLLAEGSLTISFIPVFTETLEKQGKDEAKKVSDIVFTILFMILIAV
                     TVLGIVFSPFIVKLFAYGFDQETFEVAVSLNRVMFPYIFFVSLAALCMGVLNSLRMFF
                     APAFSPVLLNLGMIATVFALHSHFKLPIYSAAIGVIIGGILQLVQQFPYLYVKGFMFS
                     FRKNLRHPAVKRIALLLVPQLFGLAVYNLNLIVSSQYASYMSAGTISYLYYAERLTEF
                     PLGIIAVSIATVLLPSLSSDVSKGNFAQFHDTYNFTLKLLLLILIPSLAGLIALRVPI
                     CNVLYQRGEFTYEATLATAQTLSGYCLGLWAVGGLRVTAPAFYAMQDTKTPVVVAFFA
                     FILNAALGYILGFTLKLEHTGLAIANSCSSIFNFLALIYLLERRTGDLKARTVVYFCL
                     KMILISAVMAGAAWWVSTYADWTGSAFSVKKIAVLALSIGVSGIVFAALTKALRIEEA
                     GFLLNLLRRRMPAGKEG"
     gene            complement(4351..>5099)
                     /locus_tag="VFJ67_02110"
     CDS             complement(4351..>5099)
                     /locus_tag="VFJ67_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HET7289205.1"
                     /translation="DYPNDPGCASTNDDDETNAQPGGGDTLSVNVKINDDWGAGYCAQ
                     VTVTNNGSQVSDWKVTFTVEGKVRDLWNASYSQSGNTVTAEGVSWNNTVNPGQSVDFG
                     FCADRTGAIPTPAPTPVPTPKPTPVPTPVPTPKPTPKPMPVPTPVPTPVPTPKPTPAP
                     TPAPSGGLTTTVRINDDWKAGYCADVTVKNTGSGNVDWVVTFDIQGKVRNMWNATYTQ
                     TGSNVRAEGVLWNNIVRAGSSVSFGFCALR"
BASE COUNT         1059 a         1414 c         1321 g         1305 t
ORIGIN      
        1 attcgcggcg agcaccttcg agacctcggc cgggttcttt accgcgtagg ggttggcgaa
       61 cacggattta atctcgacgt tgacggcgag ctccttcatg ttgcggtcga gtatcctccc
      121 gcggcgcggg agaagtgtcg agctgccctg gtgttgtttc ctcgctatgg tgaacgcgcg
      181 gtcgcggtcg aggacctgga ggtcgagcgc ttttatgcac acggctgtga aaagcacgag
      241 gacgaaggtg ccgacgatgg taatcttcca tttaggcctg tcgaggtctc ttccgagcga
      301 gatccttttc gagctcttct tcgtgccctt caccggcttc atttctttcc gtccgctaga
      361 gtggtctgct ctatgtaaat aatgtcttcc tgcgtcggat atttgaaccc catctcaagc
      421 gcggtcttct cgaggctgtc aggcgacttt gccctcataa actccgactg aagtatccgc
      481 ttctccttga gaagctcgcc cgtctgtttg ttgttccgtg aaatttcgta gcctatcctg
      541 agctcctcga ccttgaacct gacatagaag agcgcgagag cgagagcgat cagcaccagt
      601 atcacgaacc ctgtcgagat gcccaccctt tcagtcttct ccttttccct cgacccgagg
      661 gccttgacgt gggccatggc tacacctttt ccccaaccct cattttggcg ctccgcgccc
      721 tggggttgtt ctgaatctct tcctcggacg gtattatcgg cgtgcgcgtg ataatcctga
      781 gctccttctt cttgccgcag ccgcactcgg gcatagccgg cggacatacg cagggcgagg
      841 acaatctgag gaagctcgtt ttcacgagcc tgtcctcgag cgagtggaac gatatgatga
      901 cgagcctgcc ccccggcttg aggcgtgaaa cggccttccc gatgaactcc tctatattct
      961 cgagctcgtt gttgacggcg atacggagcg cctggaaggt cttggtcgcg gggtgtatcc
     1021 tggcggggtg gaacttcttc ggtatggcga gggacacgat cctggcgagc tcggccgagg
     1081 tcttgatagg actctccgcg cgcgcctcga ctattctctt ggctatcctc ctcgaccatt
     1141 tctcctctcc gtacatcttg aggaccctcg atatctcctc catcgtcatt tcgttgacga
     1201 ggtcgtaagc ggtaaatctc agtctcgggt ccatcctcat gtcgaggggc tcttccctca
     1261 tgaagctgaa tcccctctcg ctcgaatcga cctgatagga ggacatgccg aggtcggcca
     1321 gtatgccgtc gacctccctt atgtcgagac cgtccaggac cttgtccatg tctgaaaaat
     1381 tactattgcg gtaaatcacg tggccgttat acgcagagag cctttcgcgc gagatggata
     1441 tcgcctcctc gtccacgtcc agcccaatga cgagagattt cgaatgcgtg ctgtcgagta
     1501 tggatttcgt gtgtccgccc atgcccagag tggcgtccac gtatacgcct tcgggacggg
     1561 tgatgagata gcttatggcc tcatcgctca acaccgactg atggatcggt ttcgatattt
     1621 ccacgaccgc agtttccatg tccttatagt ccttgtcccg caagaatctc cctgctcttt
     1681 ttgaattctt cgtacgcgcg gtcctgctct tcctgctccc agacttcctt tgcccagatc
     1741 tctatcctcg tgagcatgcc tatcatgatg acctctttat ctatgcgggc atggttcctg
     1801 agcggctggg gtataaggac cctgccctga ccgtcgagag gacagtccac ggcccctccc
     1861 atgaggtatc tcaggaacga gatgacctcc tgcttgaact gggggaggga ggaaacttta
     1921 gtctctatgt cgttccactc cttgaggggg tacgccacga ggcatttgtc aaaattagta
     1981 atgatgagag tctcgtccgc gtacttttcc ctgcacacct cacggaacct ggcagggata
     2041 ctcacgcgtc ctttatcggt catcgtatgt tcgtatcgtc ctcggaacat atcataccat
     2101 cttatgccac tttatgccac tcaccaccac tatataactt cgtcttaagt ttgtcaagta
     2161 aggttgtgtt atttttcaat tttttagtct gtccgggggg ttgaaataaa aacatacgta
     2221 tttaacttat gtcttggact aaaaataacc gtttttcagg catcctggtt agcgctttct
     2281 ttaatattgc attgtaactt acagtaatta cattatatta tattcacgaa attgaagggc
     2341 ataaaaatac aatgatgcca cttcatacca cacgataact caagtcgtat gacgatatag
     2401 attctacctg cgaaaaatgg agatgaggaa tcggaaaagg aaaaaaaatt gtatataatc
     2461 ttctacttta cgataaacag acccggatta tgcctaccat atcgataaaa ctcaggaata
     2521 acagcgaaag cgtgtatttc cagtcgataa tgggcactta tgcccacgtc gcatgggtga
     2581 gaacggacga cccggaatcg gggatcattc aggtgataac cactcccgac cttttggaag
     2641 aaacacgtaa catactagaa agattaagaa aagagataga attcgaggag acgggcccga
     2701 acggggagac accgccgccc gtcaggcgat cataagcatg ggagaaaaag agcacatagc
     2761 gcggtcagta ggcatcgtcg gcttcataac cgtcatcagc cgcatagcgg gatatctgag
     2821 ggatatgcag atggcgtatt tcttcggggc gacaaatttt acagacgcct attggatcgc
     2881 gttcaggata ccgaacctcc tgaggaggct cctggcggag ggctccctca cgatctcgtt
     2941 catccccgtt tttacggaga ccctggaaaa acagggcaag gacgaggcaa aaaaggtctc
     3001 cgatatagtg ttcacgattt tgtttatgat cctgatcgcg gtcaccgttc tcgggatcgt
     3061 cttttccccc ttcatcgtca agcttttcgc gtacggcttc gatcaggaga cgttcgaggt
     3121 cgcggtcagt ctcaaccgcg tgatgttccc ttatatcttt ttcgtgtcgc tcgccgccct
     3181 ctgcatgggt gtgttgaact cactcagaat gttcttcgcc cccgcatttt caccggtgct
     3241 gctgaacctc gggatgatcg ccaccgtatt cgccctgcat agtcatttca agctgcctat
     3301 ttactcggcc gcaataggcg tcataatcgg cggaatcctt cagctcgttc agcagtttcc
     3361 ttacctttat gttaaaggct tcatgttcag cttcagaaag aaccttcgcc accccgccgt
     3421 caagcgcatc gctcttctcc tcgtacccca gctcttcggg ctcgcggtct ataacttgaa
     3481 cctgatcgtg agctcccagt acgcctccta catgtcggcg ggaacgatat cgtatcttta
     3541 ctacgccgag aggcttacgg aattcccgct cggaataatc gcggtctcta tagcgaccgt
     3601 gctcctgccg agtctctcga gcgacgtgag caagggcaac ttcgcgcaat tccacgatac
     3661 gtataatttt acgctgaagc tccttctgct gatactcatc ccttcgctcg cgggcctcat
     3721 agctttgaga gttccgatat gcaacgtcct ctaccagaga ggggaattta cgtacgaggc
     3781 tactcttgcg acggcccaga ccctttcggg ctactgtctc ggcctctggg cagtaggggg
     3841 actgcgggtc acagcacccg ctttttacgc gatgcaggat acgaaaactc cggtcgtggt
     3901 cgcgttcttc gcgttcatac tgaacgctgc gcttggatac attctcgggt tcacgctcaa
     3961 gctcgagcac acgggcctcg caatcgcgaa ttcatgctct tccatattca acttcctcgc
     4021 cctcatatac cttctcgaaa ggagaacggg ggacttaaag gcgaggacag tcgtctattt
     4081 ctgtctcaag atgatcctta tctccgccgt gatggcggga gcggcgtggt gggtatcgac
     4141 ctacgccgac tggacgggat cggccttttc tgtcaagaag atagccgtgc tcgcgctatc
     4201 gataggggtt tcaggaatag tgttcgcagc ccttacgaaa gccctcagga tcgaggaggc
     4261 cgggttcctc ctgaacttgc tgaggagaag gatgccggcc gggaaagagg gttgagtaat
     4321 gctccttccc ggctaataca gattcgtaat ttaccttaga gcgcagaatc cgaaactgac
     4381 cgagcttccg gcgcgcacta tgttattcca caagacccct tcagccctga cattgctccc
     4441 cgtctgtgtg taggtagcgt tccacatatt cctcaccttc ccctggatgt cgaatgtcac
     4501 aacccagtcc acgtttcctg agcccgtgtt ctttaccgtc acatccgcgc agtagcccgc
     4561 tttccagtcg tcgtttatgc ggacagtcgt cgtgaggccg cctgagggtg caggtgtagg
     4621 cgcaggggta ggcttcggag tcggcacagg cgtcgggaca ggagtgggta caggcatagg
     4681 cttaggagtg ggctttggcg tcggcacggg cgtgggtaca ggcgtcggtt tcggggtcgg
     4741 cacgggcgtg ggcgccggag tagggatagc gcccgttctg tcagcgcaga acccgaaatc
     4801 aacagactgt cctgggttga ccgtattgtt ccatgacaca ccttcggccg tgacagtatt
     4861 gcccgactga gagtaagatg cgttccagag gtctctgacc ttgccctcga cagtgaacgt
     4921 caccttccag tctgagacct gtgagccgtt attagtcacc gtcacctgag cgcagtagcc
     4981 agcaccccag tcgtcgttta tcttgacgtt cacggataga gtatcccctc cgccgggctg
     5041 agcgttagtc tcatcgtcat cattggttga cgcacacccg gggtcgttcg gataatcgg
//
LOCUS       DASSJH010000100        14583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_132303, whole genome shotgun sequence.
ACCESSION   DASSJH010000100 DASSJH010000000
VERSION     DASSJH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14583
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132303"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..2045
                     /gene="uvrC"
                     /locus_tag="VFJ67_02115"
     CDS             228..2045
                     /gene="uvrC"
                     /locus_tag="VFJ67_02115"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET7289206.1"
                     /translation="MGISDEKVGSVPVSAGVYILKDRKGRSLYIGKAKNLRSRVMSYL
                     HEGGDPQRPHISYLMREVEDLDYFVTRNEREALVLENSLIKQKKPRYNIRLRDDKNYL
                     SLRLDPRERFPRLTLTRRVLKDGALYYGPFASADALKKAKRLIHKVFPLRDCTDEKFR
                     RHSARPCLSYYMGMCLGPCAGKVGEDEYGEAVERTRMFLRGEKNSIIEMLRNSMRKAS
                     EEMRYEDAAHYRDQARLLEKNLDEQMFITPGTKDRDIVGFHREGQDAEFSVLFSRGGM
                     LVDKAEYSFKNAAGTDEDLLGEFISQFYGGNRFIPNEIIIPIGVEGIEDVSEWLSERL
                     GRKVALSVPERGVKASQLELAGRNALESFQRKHARELSELDILERLKSSLHLSRQPAA
                     IECFDISNIQGELAVASLVRFEGGKPARERYRTYKIKTVEGPNDYAMMREVLSRRLTR
                     AEEEVWGIPDLILIDGGKGQLNIAHSVIEELGYTGELDLASIAKGRYEGESDKMYIYG
                     RKNPIVFSRNSQVLFLLMRVRDEAHRFAITFHKKLRGKRAVSSALDDVPGIGAKRKKE
                     LIKRFGSLSGIKAASVDEIAAVPGLSRRIAEELKEKLSD"
     gene            2157..2621
                     /locus_tag="VFJ67_02120"
     CDS             2157..2621
                     /locus_tag="VFJ67_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7289207.1"
                     /translation="MSKVKPIPDGMNTVTPHLVCKDAARAIEFYKKAFGAEEGGRLTT
                     PDGKKVLHASLRIGDSALMLADEFPDWGSVGPETLKGTPVVIHLYVKDADSVFDQAVK
                     SGATVKMPIAEMFWGDRYGQLQDPFGHVWSIATHVRDVSFEEMEQASKTMCG"
     gene            complement(2632..4653)
                     /gene="uvrB"
                     /locus_tag="VFJ67_02125"
     CDS             complement(2632..4653)
                     /gene="uvrB"
                     /locus_tag="VFJ67_02125"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET7289208.1"
                     /translation="MESGFRIKSEFSPTGDQPGAIAELTEGVLRGDRHQILLGVTGSG
                     KTFTIANVIANVGRPTLIMAHNKTLAAQLYGELRDLFPDNPVRYFVSYYDYYQPEAYI
                     PSTDTYIEKDAQINEHIDRLRHASTTALFERRDVIIVASVSCIYGIGAPEHYYGLLTM
                     VEDGMELERDKLLEKLTEVQYERTGLDLWRSSFRVKGDTVDVFPSHHDNTALRIEFFG
                     DRVDSIKEIDPLSGKTIRPVKKAAIYPGSHYVAPRDSYERAIEGIKKELEERLAYFRD
                     RGMALEKQRLEEKTKFDLELLANMGFCSGIENYSRHISGRLPGQPPWTLLDYFPKDFL
                     LVIDESHQMIPQLRGMYEGDRSRKLSLVEHGFRLPSAIDNRPLNFAEFEKRVNQVIYV
                     SATPGPYEIEKAGGVIVEQIIRPTGLADPEVEIRTADRQVDDLLEEIQKRVAAGERIL
                     VTTLTKRMAEDLTEYYRELGIKVRYLHSDIDTLERIAIVRDLRLGKFDVLVGINLLRE
                     GLDIPEVSLVAVLDADKEGYLRSETSLIQIFGRAARNVNGKVVLYADRTTASMASAIS
                     ETDRRRSIQEKYNKENGITPSSIKKSVTDILSTIYEADYYTVPLEKTPEPIDIPPEKI
                     SKTISALEREMKEAARSMEYETAAQKRDQIKRLRELEIKYLGSSEENPQ"
     gene            4654..9117
                     /locus_tag="VFJ67_02130"
     CDS             4654..9117
                     /locus_tag="VFJ67_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7289209.1"
                     /translation="MDEFSIAPAAGVKKTASSIIIITMALGIFHDSVAAWFKKEFDAP
                     TEIQERAWPEIKKRKNTLISAPTGSGKTLAAFLSAIDDLVKAGLEGRLPDKTQIVYVS
                     PLKALSNDIEKNLQRPLRGIEEELANAGVPEIGIRAQVRTGDTPAAARTRMTKNPPHI
                     LITTPESLYLILTSKNGREMLSEVHTLIVDEVHALVGSKRGSHLALSMERLESLVSGE
                     LVRIGLSATQKPIETTARFLTGTGRGGRNGAPDCTIIDTGHRRDIELSLEIPRSPLTA
                     VMSNEVWEEIYEKLEELIKSHKTTLIFVNTRRLSERMTHNLTERFGADVIAAHHGSIS
                     KELRLDAERRLKSGELKAIIATASLELGIDIGAVDLVCQIGSPKSISTFLQRAGRSGH
                     TIKGTPKGKIFPLSLDELAECAALFDAVKRGELDSIIMPEKPLDVLAQQIVAEVSCGE
                     RLEDELYEMLVRAYPYRDLSRSEFDDVVKMLAQGFAAQKGRRAAYIHHDMVNGRLRAR
                     KGSRIAAIVSGGTIPDSFDYDVILEPTGTFIGTVNEDFAIESTAGDIFLLGNHSWRII
                     KVNGGKVRVEDAGGMPPTIPFWFGEAPGRSTEFSAAVSRLREEISGKLGDIKDFIKED
                     DLSNGSPDDGWKKGAIEHLMTRAGLGVEAADQLVTYMAAVKAALGTVPTQERLVMERF
                     FDEAGAMHLVIHSPFGSRMNRAWGLALRKRFCRKFNFELQAAATEDSIILSLGATHSF
                     PLEEVYFYLHPNNVREVLTQALLDAPMFEVRWRWNASTALAVLRRWTGKKVPPAIQRI
                     HSEDLIAQVFPDQIACLENIVGDREIPDHPLVNQTIKDCLTEAMDIDNLESLLGDIHS
                     KKIELVACDLREPSPLTGQVLNARPYAFLDDTPLEERRTHAVQNRRWLDPSEAAELGQ
                     LDAGAVSTVKEEAWPEAESPDELHDALILCGFITEGEGERGDAAGSWQPYFEELVKEN
                     RAAVLETGEGKRLCAAAERLAQMKSVHPDATLITPIAIPERLLESPASREGAVVEVLR
                     GRLEALGPVTAAELAESLGVTQQDADQALLTLEGEGFVFRGSFTPGANGIEWCERRLL
                     ARIHKYTMSKLRKEIGPVTAADFMRFLFSRHGVDSEDKPEGAEALKGVLEMLEGYEAP
                     AAAWEGDILSSRMREYDHTWLDTLCLSGNVVWARIKAQSVPNKKPGPIKTTPVMIVKR
                     ANLGAWRKFAPDHVAAAENLSRRAREVLEIIEERGASFFEEIASGMRVFKSETESAIA
                     ELVSSGVITSDSYTGLRALLVSSKYRTAEGRRRKRVSFSMEGAGRWSLIRAGGGEGED
                     GRKGEDMRTLAMALLRRYGVIFRRLAERESFSPPWRDLVRVLRLLELRGEVRGGRFVD
                     GVWGEQYALPEAVAGLREARRKPREGKLVSISAADPLNLTGIITPGERVSSHYKNRVL
                     YRDGVPAAIKEGDDVRILSAADGKEEWSLRTALVRRSFSPRLRAYLGKGVA"
     gene            9253..9447
                     /locus_tag="VFJ67_02135"
     CDS             9253..9447
                     /locus_tag="VFJ67_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289210.1"
                     /translation="MSAIAADWREASRRNVDAVIERIRCAERARVAFKETACPECSYI
                     TRNVFFPAQCTYCGSSLQPL"
     gene            complement(9458..9919)
                     /locus_tag="VFJ67_02140"
     CDS             complement(9458..9919)
                     /locus_tag="VFJ67_02140"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7289211.1"
                     /translation="MSDTLKVGDEAPDIEAETYGGEKIKLGNYRGKKTIALYFYPKDY
                     TGGCTKEACSIRDGMDDLEKLGVQVLGVSTDSVKSHEGFREKHGLNFPLLSDKSKNII
                     KSYGVVSDSGSAKRETFLIDKSGVIRYIWTKVKTAEHAEEIADKAKELGLV"
     gene            complement(10235..11698)
                     /locus_tag="VFJ67_02145"
     CDS             complement(10235..11698)
                     /locus_tag="VFJ67_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET7289212.1"
                     /translation="MNDPILAAKGEHELFILPSMANRHGLISGATGTGKTVTLQVLAE
                     NFSRIGVPVFMADVKGDLSGLSRPGSENPKIAERINKIGLSNFVYGESPVIFWDVFGA
                     QGHPVRTTISEMGPLLLSRLLGLNDTQAGVLTLVFEIADDDRLLLLDLKDLRSMLRYV
                     GDNSSSFTTGYGNISQASIGAIQRNLITLENEGGDVFFGEPALDLDDFLGTDPNGRGV
                     INILAAERLIRSPAIYATFLLWLLSELFEQLPEAGDPEKPRLVFFFDEAHLLFDNAPK
                     ALVDKVEQVARLIRSKGVGVYFVTQSPLDIPDVILGQLGNKVQHALRAYTPRDQKAVK
                     AAAETFRANPALDTQKTLTELGVGEALISFLDKDGRPNIVEWALVRPPFSRIGPVTPE
                     ERGVVIGSSPLRGRYEKVTDRESAYEMLKGRAERAPAAKSAPKNPPRRRSDTVMEAMA
                     KSAARSFGRQLATAGRQATTEIIRGVLGSLLGGKRGR"
     gene            complement(11774..12610)
                     /locus_tag="VFJ67_02150"
     CDS             complement(11774..12610)
                     /locus_tag="VFJ67_02150"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET7289213.1"
                     /translation="MKFGAHISIAGGIDKAPGRAHAIGCECFQIFTRSPRGGKPPALS
                     KDLVDSFLASCSSYGLADYYIHTPYYINLASEKNGLRHSSVAIIREELERGSVIGAKY
                     VMTHLGSSKGMDRARAVDMVAEGIASILEDSDDLSTELLLENTAGQGETIGDTFEELA
                     HLLTDVGNPRLGICLDTAHLLASGYDMRTKASLLDSLAAFSSVIGLDRVKLVHGNDSK
                     AGLGEKKDRHEHIGKGKIGKEGFKAVLENPSLKKLDMIVEIPPEEVAPDIELLKRMRS
                     ET"
     gene            complement(13048..>14583)
                     /locus_tag="VFJ67_02155"
     CDS             complement(13048..>14583)
                     /locus_tag="VFJ67_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HET7289214.1"
                     /translation="LFYGDAMLTPAISVLSAVEGLRIASPVFHDYVIPITLVILTFLF
                     FYQSKGTGRVGALFGPVMLVWFFTLAVLGAMWIMKRPEVLVALNPVHAVEFFIANHVH
                     GFLVLGAVFLVVTGGEALYADMGHFGGFPIKLAWFALVLPSLLLNYFGQGALILARPE
                     ATENSFYLLAPPWGLYPLIVLATLATIIASQAVISGAFSLTSQALQLDFLPRVRVVYT
                     SPEQMGQIYIPFVNWLMFFGTIFLVVVFRSSGNLASAYGAAIVTLMVITTLLMYVCSV
                     SKWKWNVLIAIAVTAGLLIVDLAFFGANIIKINQGGWFPFLIAILVYLVVITWTRGRR
                     ALAEEFRKEEISFDLFLSDPGLKSVTRVPGTAVYMSKNPDGVPRTMLHNFKHNKVFHE
                     RMIILTIYTEGVPRVPWPKKFKIEKLEHNFIRVIAYYGFMETPDISHVVTYLKDHGID
                     CSVQEITFFLGRETIVVRDPRGITGLRKQLFAFLSQNMLPATSHFNVPPNRVIEIGLR
                     IQI"
BASE COUNT         3247 a         3735 c         4457 g         3144 t
ORIGIN      
        1 gtttgagaag gggctgaaga atctaaaaaa cgtagcaaag aaagagagtt agcctgtata
       61 tttccacagt tacggatacc tacggcgtcg ggaaaagaca aaatatgaga ccctgaaact
      121 agttcagggc gacgacgaaa caagacacat cacgatttga tcaagcgcgc agtcccggat
      181 tttccgatgt ttgcttaaga aagaaattcc tctaacctaa ataagctatg ggcataagcg
      241 acgagaaggt cgggtctgtt cccgtgtcgg ccggggtcta tatccttaag gacaggaaag
      301 gcaggtcctt gtacataggg aaagcgaaaa acctgagaag ccgcgtgatg tcgtacctcc
      361 acgaaggcgg ggatccgcag agaccccaca tctcctatct gatgagggaa gtggaggatc
      421 tcgactactt cgtgacgcgg aacgagcggg aagctctggt cctggagaat tccctcatca
      481 agcagaaaaa gccacggtac aacattaggt tgagagacga caagaattac ctctccctca
      541 ggctcgaccc ccgggagagg ttcccgcgcc tcacgctcac gaggagagtg ctcaaggacg
      601 gcgcccttta ttacgggcct ttcgcatcgg cggacgcgct caagaaggcg aagaggctca
      661 ttcacaaggt gttccctcta agggactgca cggacgagaa gttcaggcga cactccgcga
      721 ggccttgtct cagctactac atgggcatgt gtctcgggcc ctgtgcgggg aaagtcggcg
      781 aagatgaata cggggaggcg gtcgagcgga cccggatgtt cctccgggga gaaaagaaca
      841 gtatcatcga aatgcttaga aacagtatga ggaaggcttc tgaggaaatg aggtacgagg
      901 atgccgccca ctacagggac caggcgaggc ttctcgaaaa aaatctggac gaacagatgt
      961 ttataacgcc cggaacgaag gacagggaca tagtgggttt tcacagggaa gggcaggacg
     1021 ccgagttctc ggtcctcttt tcgagagggg ggatgctggt ggataaggct gaatattctt
     1081 ttaagaacgc ggccggtacg gatgaagacc tcctcgggga gttcatttcc cagttttacg
     1141 gcggcaacag atttataccg aacgaaataa ttatccccat aggggtagag ggcatagagg
     1201 atgtctcaga gtggctctcg gagaggctcg ggcggaaggt cgcgctcagc gtgccggaaa
     1261 ggggggtgaa agcttcgcaa ttagagctcg cgggcagaaa tgcgctcgag agcttccaaa
     1321 ggaagcacgc ccgggagctg agcgagctcg acatcctcga aaggctaaag tcttcacttc
     1381 acctgagcag acagcctgcg gccatagagt gcttcgatat ttccaatata cagggggagc
     1441 tggcagtggc gtcactcgtg aggttcgagg gagggaagcc cgccagggag aggtacagga
     1501 cttacaagat aaagacggtc gagggcccga atgactacgc catgatgcgc gaggtcctat
     1561 caaggagact cacgagggca gaggaggagg tgtggggcat ccccgatctt attcttatcg
     1621 acgggggaaa gggacagctg aatatagcgc atagtgttat tgaagaactc ggctatacgg
     1681 gcgagctcga cctcgcctca atagccaagg gcaggtacga aggcgagagt gataagatgt
     1741 acatatacgg caggaaaaac ccgatcgtct tctcgagaaa ctcgcaggtc ctgttcctcc
     1801 tcatgagggt gagggacgaa gcccacagat tcgcgatcac cttccacaag aagctccggg
     1861 gaaagagggc cgtaagctcc gcgctcgacg acgtgcccgg cataggggcg aaacggaaga
     1921 aggaactcat aaagcggttt gggagcctgt cgggcataaa ggccgcttct gtcgatgaga
     1981 ttgcggccgt cccggggcta agcaggagga tagccgagga gctgaaggag aagctctctg
     2041 attaatgggt gtcccgaacg cccatgccaa ttcgtcgttt tagtgaacgg ccggtaagcg
     2101 gcgccgggtt cttaccactg gcgctccggc aaaatccagc ataaggagct gtaattatgt
     2161 caaaagtgaa acccataccc gacggaatga atacagtgac gccgcatctc gtctgtaagg
     2221 acgcggccag ggccattgag ttttataaaa aggctttcgg cgcggaggag ggcgggaggc
     2281 tgaccactcc tgacgggaag aaggtcctcc acgcatcgct ccgtatagga gattcggcgt
     2341 taatgcttgc cgacgagttc cccgactggg gctcggtggg gcccgaaaca ctcaaaggga
     2401 cgcccgtggt tattcatctc tatgtcaaag acgcggattc ggtatttgat caggcggtta
     2461 aatcgggagc gaccgttaag atgccgatcg cggagatgtt ttggggggac agatacggcc
     2521 agctgcagga cccgttcggc cacgtctggt ctatagcgac tcacgtaagg gacgtgagct
     2581 tcgaggaaat ggagcaggct tccaaaacca tgtgcggctg aaggctgcgc gctattgagg
     2641 gttttcttca gagcttccga ggtatttaat ctcgagctcg cggaggcgct ttatctgatc
     2701 cctcttctgc gcggccgttt catattccat acttctcgcg gcttccttca tctccctctc
     2761 gagggcgctt atggtcttgg aaatcttctc gggcggtata tcgatgggct cgggcgtctt
     2821 ctcgagcgga acggtgtagt aatcggcctc gtagatggtc gatagtatgt cggtgaccga
     2881 ttttttaata ctcgaaggcg taatgccgtt ctctttattg tacttttcct ggatactgcg
     2941 tctcctgtcg gtttccgaaa tcgcgctcgc catagacgcc gtggtcctat cggcgtagag
     3001 caccaccttg ccgttcacgt tccgggccgc ccttccgaat atctgtatga gggatgtctc
     3061 cgagcggaga tatccttctt tatcggcgtc gagcaccgcg acgagcgaga cttcagggat
     3121 gtcgagtcct tcccggagca gattgattcc cacgaggaca tcgaatttcc cgagcctcaa
     3181 gtccctcact atggcgatcc tttcgagggt gtctatgtcc gagtggaggt agcgcacttt
     3241 tattccgagc tccctgtaat attcggtcag gtcttcggcc atccttttcg tgagagtggt
     3301 tacgagtatc ctctcgcccg ccgcaactct cttttgaatc tcctccagga gatcatccac
     3361 ctgcctgtcc gcagtcctta tctcgacttc cggatcggcg agccctgtcg gacgtattat
     3421 ctgctcgact attaccccgc ccgctttctc tatctcgtag gggccgggtg tagcggagac
     3481 gtaaatcacc tgattgaccc ttttttcgaa ctcggcgaag ttaagcggcc tgttgtcgat
     3541 cgccgagggg agcctgaacc cgtgctctac caggctcagc ttcctgctcc tgtccccttc
     3601 gtacatgccg cgaagctggg ggatcatctg gtggctctcg tctattacga gaaggaagtc
     3661 tttggggaag tagtcgagga gcgtccaggg gggctggccg gggagcctgc ccgatatgtg
     3721 cctcgaatag ttctctatgc cggagcagaa gcccatattc gccaggagct cgaggtcgaa
     3781 tttcgttttc tcctcgagcc tctgcttctc gagcgccatt cctctgtccc tgaaatacgc
     3841 gagtctttcc tcgagctctt tttttatgcc ctcgatcgcc ctttcgtagc tgtctctcgg
     3901 ggcgacgtag tgagagcccg ggtatatggc tgcctttttg acgggtctta tggtcttgcc
     3961 gctgagaggg tctatctcct ttatcgagtc cacgcggtct ccgaagaact ctatccgtag
     4021 agccgtgttg tcgtggtgtg acgggaagac gtcgacagtg tctcccttca cgcggaagct
     4081 gctcctccag aggtcgagcc ccgttctctc gtactggacc tccgtaagct tctcgagaag
     4141 cttgtcccgc tcgagctcca tcccatcctc gaccatcgtg aggagcccgt agtagtgctc
     4201 cggagcgccg atgccgtata tgcacgacac gctcgcgacg attatcacgt ccctcctctc
     4261 gaagagggct gtcgtggagg cgtggcggag cctgtctatg tgctcgttta tctgcgcgtc
     4321 tttctctatg taggtgtccg tagacggtat atatgcctcg ggctggtaat aatcgtagta
     4381 actcacgaag taccttaccg gattatcggg gaagagatcc ctgagctctc cgtaaagctg
     4441 ggcggcgagc gtcttgttgt gggccattat gagggtgggg cgtccgacgt tcgcaatgac
     4501 gttcgctatg gtgaatgtct tgccgctccc ggtgacgcct agcaagatct ggtgcctgtc
     4561 cccgcggagc acgccttcgg tgagctccgc tatggccccg ggctgatctc ccgtgggcga
     4621 gaactcgctc tttatccgga aaccgctttc catatggatg agtttagcat agcgcccgcg
     4681 gccggtgtta aaaagacggc ttcatcgatt ataataatta ctatggcttt gggaatattc
     4741 cacgattcgg tagcggcgtg gtttaaaaaa gagttcgacg ctcctacgga gatacaggaa
     4801 agggcctggc ccgagatcaa gaagcggaag aacaccctta tatccgcccc gaccggttcg
     4861 ggtaaaaccc ttgccgcatt cctttcagcg atcgacgatc tggtgaaggc gggcctcgaa
     4921 gggaggctcc cggataaaac gcagatcgtt tacgtctccc ctctcaaggc cctgagcaac
     4981 gatatagaaa aaaaccttca gaggccgctc agaggcatag aggaagagct ggcgaatgcg
     5041 ggggttccgg agatcggcat aagggcccag gtcaggacgg gcgatacgcc cgccgcggcc
     5101 cggaccagga tgacaaagaa ccctccccat atattgataa ccacaccgga gtccctctac
     5161 ctgattctca cgagcaaaaa cgggcgtgag atgctgtcgg aggtccatac gctcatagtg
     5221 gacgaggtgc atgccctggt cgggagcaag cgcggctcgc acctggcgct ctcgatggag
     5281 agactcgaat ccctcgtatc aggagagctc gttcgcatag gactttcggc gacgcagaag
     5341 cctatcgaga caacggcgcg tttcctcaca ggcacgggca ggggagggag gaacggagcg
     5401 cccgattgta ccataataga taccggccac aggagagaca ttgaactgtc tctcgagatc
     5461 ccccgctctc cccttaccgc ggtaatgtcg aacgaggtat gggaggagat atacgaaaag
     5521 ctcgaagagc ttataaaatc ccataaaacc actttgatat tcgtaaacac gagaaggctt
     5581 tccgagcgca tgacgcacaa cctgacggag aggttcggcg ccgatgtcat cgccgcccat
     5641 cacggaagca tatccaagga gctgcggctc gacgcggagc gaaggctcaa atcgggcgag
     5701 ctcaaggcga taatcgcaac ggcgtcgcta gagctcggta tagacattgg cgcggtcgat
     5761 ctcgtgtgcc agataggctc tccgaagtcg atctcgacgt ttctccagag ggcggggcgc
     5821 tctgggcata cgataaaggg caccccgaag ggcaagatat tcccgctcag cctggatgag
     5881 cttgcggaat gcgccgcgct cttcgacgcg gtgaagcggg gcgagctcga cagcataatc
     5941 atgcccgaga aaccgctcga cgtgctcgcg cagcagatag tggcagaggt ttcgtgcggg
     6001 gagaggctcg aagacgagct ctacgaaatg cttgtgaggg cttacccgta cagagacctg
     6061 tcacggagcg agttcgacga cgtcgtaaag atgctcgctc aggggttcgc ggctcagaag
     6121 gggaggcgcg cggcgtacat acaccacgac atggtgaacg ggcgcctccg tgcgaggaaa
     6181 ggctcccgca tagccgccat agtatcgggc gggacaatac ccgacagctt cgattacgac
     6241 gtgatactcg agccgacggg aacttttata ggcactgtga acgaggactt cgcgatagag
     6301 agcaccgcgg gggacatatt cctgctcggg aaccattcat ggcggatcat aaaggtaaac
     6361 ggcgggaagg tgagggtgga agacgccgga gggatgccgc ctacgattcc attctggttc
     6421 ggcgaggccc cggggaggag cactgaattc tcggcggcag tttccaggct gagggaagag
     6481 atatcgggaa agctcggaga tataaaagat tttataaaag aagacgactt gtcaaacggt
     6541 tccccggatg acggctggaa gaagggggcg atcgaacacc tcatgacgag agccgggctt
     6601 ggggtggagg cagccgatca gctcgttact tatatggcgg ccgtgaaggc cgctctcggc
     6661 acggtgccga cgcaggaaag gctcgtgatg gagaggttct tcgacgaagc gggcgcgatg
     6721 cacctcgtca tacattcgcc attcgggagc agaatgaacc gcgcctgggg gctcgcgctc
     6781 cgaaaacggt tctgcaggaa gttcaatttc gagcttcagg ccgctgcgac cgaggattcc
     6841 ataatactga gcctgggggc gacgcacagc ttccccttgg aagaggtcta tttctatctc
     6901 catccgaata atgtgaggga ggtattgacg caggcgctcc tcgacgcgcc gatgttcgag
     6961 gtaaggtggc gctggaacgc gagcacggcg ctcgcggtac tgagaaggtg gacggggaag
     7021 aaggtgcctc cggcgatcca gcgcatacac tcggaggacc tgatcgccca ggtgtttcct
     7081 gaccagatag cgtgcctcga aaacatagtc ggcgacaggg agataccgga ccatccgctc
     7141 gtgaaccaga ccataaagga ctgcctcacg gaggcgatgg atatagacaa tctcgaaagc
     7201 ctccttggag atatacattc gaagaagata gagttggtcg cttgcgacct gcgcgagccg
     7261 tcgccgctca cggggcaggt acttaacgcg aggccttacg cgtttctcga cgatacgccg
     7321 ctcgaggaga ggaggacgca tgcggtccag aacaggcgat ggctcgatcc ttcggaggcg
     7381 gccgagctcg gacagctcga tgcaggtgct gtaagtacgg tgaaggaaga ggcgtggccc
     7441 gaggctgaga gcccggacga gctccatgac gcgctcatac tctgcggatt cataacggaa
     7501 ggggaggggg aaaggggaga cgcggctggc agctggcagc cttatttcga agagctggtt
     7561 aaagagaaca gggccgcggt gctggaaacg ggagagggga aaagattatg tgcggcggcc
     7621 gagaggctcg cgcaaatgaa atccgtgcat cccgatgcaa cactgataac acccatagct
     7681 attcccgaga gattattgga gagccccgcg tcgagagaag gggcggttgt agaggttcta
     7741 agggggcgtc ttgaggcgct cggccccgtt acggcggccg agcttgccga gtcgctgggc
     7801 gtcactcagc aggatgcgga tcaggcgctc ctgactctcg agggggaagg gtttgtgttc
     7861 agggggagct ttaccccggg ggcgaatggg atcgagtggt gcgagaggcg gctcctggcg
     7921 aggatccata aatatacgat gagcaagctc cggaaggaga tagggcccgt tacggcggcc
     7981 gatttcatga ggtttctttt ctccaggcac ggggtcgact cggaggataa gcccgaaggg
     8041 gctgaggcgc tcaagggcgt gctcgagatg ctcgaaggat acgaagcccc ggctgctgcc
     8101 tgggaggggg atatactgtc ctcgaggatg agggagtacg accatacgtg gctcgatacg
     8161 ctatgtctat cgggaaatgt agtgtgggct aggataaagg cgcaaagcgt cccgaacaag
     8221 aagcccgggc cgataaagac cacgcccgta atgatcgtaa agagagcgaa cctcggggcg
     8281 tggaggaaat tcgccccgga tcacgtagcg gccgcagaaa atctgtcccg ccgggcgagg
     8341 gaagtgctcg aaataatcga ggagcgagga gcgtccttct tcgaggagat agcgtccgga
     8401 atgagagtgt ttaaatcgga gacggagagc gcaatcgcgg agctcgtttc gagcggcgtc
     8461 atcacgtccg acagctacac ggggctgaga gcgcttctcg ttagctctaa atacagaact
     8521 gccgaaggaa ggcggaggaa aagggtgagc ttcagcatgg aaggggccgg caggtggtct
     8581 cttattcggg ccggaggagg ggagggagag gacgggagga agggcgagga tatgcggacg
     8641 ctcgccatgg ctctactcag aagatacggg gttatattca ggaggctcgc tgagcgtgag
     8701 agcttctctc ctccgtggag ggatctcgtg cgggtgctga ggctcctcga actgaggggc
     8761 gaggtaaggg gagggaggtt tgtggacggt gtgtggggtg agcagtacgc gctgccggag
     8821 gcggtcgcag ggttgagaga agcgagaagg aagccgaggg agggaaagct cgtttcgata
     8881 agtgccgccg acccgctcaa tctgacaggg attataacgc ccggggagag ggtgtcgtca
     8941 cattataaga acagggtgct ttacagggac ggagtccccg ctgccattaa agagggggat
     9001 gatgtgagaa tcttgtcggc tgcagacggt aaagaggaat ggagcctcag gacggcgctc
     9061 gtgaggcgca gcttttcacc caggctgagg gcgtatctcg gcaagggtgt cgcttaacaa
     9121 aggaagcaat ctaactaaaa cagttaaccc gcatttaact ttttcataat ccgctcttaa
     9181 tttctccgtg tttaaatttt ggtaatgggc gttgtgtggg tttggaatcc gaaggcaccc
     9241 gagacgggag atatgagcgc gattgccgcc gactggaggg aagcgagcag gcggaatgtg
     9301 gatgcggtta tagagaggat caggtgcgcg gagagagcgc gggtcgcttt taaggaaacc
     9361 gcgtgcccgg aatgcagtta tataacacgc aatgtctttt tccccgctca gtgtacctac
     9421 tgcggcagct cgctccagcc cctatagttc gggacgctca gacgagtccg agctcttttg
     9481 ccttatcggc gatctcctct gcgtgctcgg ccgttttgac cttcgtccag atgtaacgta
     9541 ttacgcctga tttgtcgatc aggaaagtct cccgctttgc cgagcccgaa tcgcttacga
     9601 cgccgtacga tttgattata ttcttgctct tgtcgctgag gagcggaaag ttaagaccgt
     9661 gtttctccct gaagccttcg tgggatttca cgctgtccgt gctgactccc aggacttgaa
     9721 cgccgagctt ctcgagatcg tccattccgt ccctgatcga gcaagcctct ttggtgcatc
     9781 cgcccgtgta gtccttcgga tagaagtaga gcgcaatcgt tttcttaccg cggtagttac
     9841 cgagctttat tttttcgcct ccgtacgtct cggcctcgat atcaggtgcc tcgtccccga
     9901 ctttcaatgt gtctgacatt acgggattcc tcctttcgcg atttccgctt tgagtatagc
     9961 tgatgaagaa ggttgaggaa gggggcggaa atgccgcgga gtttccccca tatattaaag
    10021 ccgagtatta aatattcttc gtttatccac acctattaag gtttcggttt aatacatgat
    10081 tttaacccat caatataatt tgtagggagc cgtctcttgc gaaacgatgg ccgagtttct
    10141 caatttatga tggttgaata gtaacgattt ttgagacgga gaattaacat ggtgcgttac
    10201 tgacgtgacg cgaattgaca aagctgcgga ctgtttatct gccgcgcttt ccgccgagaa
    10261 gcgatcccag cactccgcgg ataatttccg tggtcgcctg tcttcctgcg gtggccagct
    10321 gtctgccgaa agaacgggcc gcgctcttgg ccatcgcttc catgaccgtg tcactcctcc
    10381 ggcgcggagg gtttttgggc gcggattttg ccgcaggcgc tctctccgcg cggcccttca
    10441 acatctcgta tgcggactct ctgtcggtta ctttttcata acgtccgcgg agaggcgagc
    10501 ttccgattac gacgccgcgc tcttcagggg ttacggggcc tatcctgctg aacgggggac
    10561 gaacaagcgc ccactccacg atgttcggcc ttccgtcctt gtcgagaaac gatatgagcg
    10621 cctcgccgac gccgagctcg gtgagtgtct tctgggtgtc gagggcggga ttggctctga
    10681 aagtttcagc tgctgccttg accgccttct ggtcgcgcgg tgtatatgcg cggagcgcat
    10741 gctggacctt gttcccgagc tggcccagaa tgacgtccgg tatatcgagc gggctttgag
    10801 tcacgaaata cacgccgaca ccctttgagc gtatgagacg ggcgacctgc tcaactttat
    10861 cgacaagagc cttgggcgcg ttgtcgaaga ggaggtgggc ctcgtcgaag aagaatacca
    10921 gcctgggctt ttcggggtcg cctgcttcgg gcagctgctc gaacagctcg gaaaggagcc
    10981 agaggaggaa cgtggcatat atagcgggtg agcggataag tctctcggcg gcgaggatgt
    11041 taatgacgcc gcgcccgttg gggtctgtcc cgagaaaatc gtcgaggtcg agggcgggtt
    11101 ctccaaagaa cacgtcgccc ccctcgtttt cgagggtgat caggtttctt tgtatcgctc
    11161 ctatacttgc ctgggagatg ttcccgtagc cggtcgtgaa cgacgaggag ttgtctccca
    11221 cgtagcggag catcgatctc agatctttga ggtcgaggag gagaagtctg tcgtcgtcgg
    11281 cgatctcgaa gacgagcgtc aggaccccgg cctgggtatc gttcaggccg aggaggcggc
    11341 tcaggaggag cggacccatt tccgatatcg tcgtgcggac ggggtggccc tgtgcaccga
    11401 agacgtccca aaagatcacc ggagattcac catatacgaa attcgagagg ccgatcttgt
    11461 taatgcgctc tgcgatcttc gggttttcgc tcccaggccg ggagagtccg gagagatcgc
    11521 ccttgacgtc ggccatgaac accgggaccc cgatcctgct gaaattctcg gccaggacct
    11581 ggagggtcac ggtcttgccg gtcccggtgg ccccggagat aagcccgtgc ctgttcgcca
    11641 tcgaggggag gataaagagc tcgtgctctc ctttagctgc caatatagga tcgttcatgc
    11701 ctttataatt ccttttacga gtgttgttgt aaagcgggag tgcgaagcgc cccgtctcat
    11761 ccgggcgcgg gagtcaggtt tcacttctca tacgctttaa aagctcgata tcgggagcga
    11821 cttcttcggg cggtatctcg acaatcatat cgagcttttt gagggaggga ttctcaagca
    11881 cggctttgaa cccctccttt ccgatctttc ccttgcctat gtgctcgtgc ctgtcctttt
    11941 tctctccgag tccggccttc gaatcgtttc cgtgaacgag cttcacccgg tcgaggccaa
    12001 taacggagga gaatgcggcg agtgagtcca agagagaggc tttggtcctc atatcgtagc
    12061 cggaggcgag gaggtgcgcc gtgtcgagac atatgccgag cctcgggttt ccgacatcgg
    12121 tgaggaggtg ggctaattcc tcgaacgtat cccctatagt ttctccctgg ccggccgtgt
    12181 tttcgaggag gagctctgtg gagagatcgt ccgagtcttc gagtatcgag gcgatgcctt
    12241 ccgcgaccat gtcgacggcc cgggcgcggt ccataccctt cgagctgccg aggtgcgtca
    12301 tgacgtattt agcgcctata acgcttcctc tttcgagctc ttcccggatt atggcgacgg
    12361 aggagtgcct gagcccattt ttctcggacg cgaggttgat gtagtagggc gtgtggatgt
    12421 agtagtcagc aagaccgtac gaggagcagg atgcgaggaa agaatcgacg aggtccttgg
    12481 aaagcgccgg gggtttccct ccccttgggg agcgcgtgaa tatctggaag cactcgcatc
    12541 ctatcgcatg agcgcggccg ggagctttgt ctataccgcc cgctatggat atgtgcgcgc
    12601 cgaatttcat aaggtcgtaa ttttaccgga ttcggtgggg aaaaggattc gagatggaaa
    12661 atcttaattc cgctattgca atgtattttc atcacaatta aacaattact cattaaaaaa
    12721 agatattttt gtgcgcgaaa tgctttctct ttaaaaaata gattattgtg caatagcggt
    12781 attagatgcg tgtgcaaccg cttcacatgg ttacaacttg tcatgtcaga taaggtatct
    12841 tatgttaact ataatattgg actgcttttt ggatggagtg ccttgcctcc gtccgccgga
    12901 ttgatttcga gttacataac atgcttttat attcgatcgt caagccgtga cgagccgata
    12961 agtaaaatta ttgtacgccc tcaccccgac cctctctcct cctactgaga gagggagatt
    13021 aaaaggaaga tgcatcccgg ttttttatca gatctgtatg cggaggccga tctcgatcac
    13081 gcggttggga gggacgttga aatgggaagt cgccgggagc atgttctggg acaggaaggc
    13141 gaagagctgt ttccttagtc ccgtgatccc cctcggatcg cgaacgacta tggtttcccg
    13201 cccgaggaag aacgttatct cctggacgct gcagtcgatc ccgtgatcct tgagatacgt
    13261 tacgacgtgg gatatatcgg gcgtctccat gaacccgtaa taggcaatta cgcggataaa
    13321 gttatgctcg agcttctcga tcttgaattt cttaggccac gggacgcgcg gcacgccttc
    13381 cgtgtatatg gtcaggatta tcatgcgctc gtgaaagacc ttgttgtgct tgaagttgtg
    13441 gagcatggtg cgcggcacgc cgtccgggtt cttggacata tagaccgccg ttccgggaac
    13501 gcgagtgact gacttgaggc cgggatccga taaaaagagg tcgaacgata tctcctcctt
    13561 ccggaactcc tccgcaagcg ccctcctccc tctggtccac gtaattacca ccagatacac
    13621 cagtatcgca ataaggaacg ggaaccaccc gccctggttg atcttgatta tattcgcccc
    13681 gaaaaacgcc aggtccacta tcagcagacc ggccgttacg gcgatcgcta tgagcacgtt
    13741 ccacttccat ttcgaaaccg agcacacgta catgaggagc gtggttatca ccatgagcgt
    13801 aactatggcg gccccgtagg cgctcgcgag gttgccggac gagcggaaca ccaccacgag
    13861 aaagatcgtg ccaaaaaaca taagccagtt aacgaagggt atgtatatct gtcccatctg
    13921 ttcgggggac gtgtatacga ccctgacgcg agggaggaaa tcgagctgaa gagcctggga
    13981 cgtaagcgaa aatgctcccg agataaccgc ctgagaggcg ataatggtcg ccagggtcgc
    14041 aagcacgatg agcggataga gtccccacgg aggcgcgagg aggtagaacg agttctccgt
    14101 agcttcgggc cttgcgagaa tcagcgcgcc ctgtccgaaa tagttgagaa ggagcgaggg
    14161 gagcacgagc gcgaaccagg cgagctttat aggaaacccg ccgaaatgtc ccatgtccgc
    14221 gtacagcgcc tcgccgcctg tgacgaccag gaacacggcc cccagcacta aaaatccgtg
    14281 tacatggttc gctatgaaga attccacggc gtggaccggg ttaagcgcca cgagcacctc
    14341 gggacgtttc ataatccaca tcgccccgag aacggcgaga gtaaaaaacc atacgagcat
    14401 tacgggacca aagagggcgc ccacgcggcc cgtgcctttg ctctggtaaa agaacaggaa
    14461 cgtaagaatc acgagggtta tcgggatcac gtagtcgtgg aatacgggtg aggcgatcct
    14521 gaggccctcg accgcgctta gaaccgatat tgcgggtgtc agcatggcgt ctccgtagaa
    14581 gag
//
LOCUS       DASSJH010000101         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1212870, whole genome shotgun sequence.
ACCESSION   DASSJH010000101 DASSJH010000000
VERSION     DASSJH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212870"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFJ67_02160"
     CDS             <1..407
                     /locus_tag="VFJ67_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007947151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7289215.1"
                     /translation="CFCGAVEISVTGEPLAMGYCHCDSCRHWSAGPVSTYTIWAPGSV
                     EVTKGAGNIGTYNKTEFSYRKWCKSCGGHLYAEHPSINLTDVPADILPDLTFKPTAHL
                     NYQETVLPMKDGLTKYRDFPKEAGGSGETMEE"
     gene            594..929
                     /locus_tag="VFJ67_02165"
     CDS             594..929
                     /locus_tag="VFJ67_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7289216.1"
                     /translation="MLRNQIISSVAVILTFGFFFLALPEEGFSGLSTQINCCQLEGQC
                     EDTTQGPFMCTEEDFVPNAVCDQGTGSCVLISRINPIPTVSEWGLIAMAGILGIAGFI
                     IVRKRKVAA"
     gene            1245..1679
                     /locus_tag="VFJ67_02170"
     CDS             1245..1679
                     /locus_tag="VFJ67_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7289217.1"
                     /translation="MSESKTYKGTCFCGAVEIAVTGDPALMGYCHCSSCRHWSAGPVN
                     AFTLWNPEAVKYTKGGDNVLTYNKTEFSFRKSCKTCGGHVLTEHPTFGLTDVYAAIIP
                     DLQFKPAVHVNYQETVLHMKDGLPKFKDFPKEAGGSGETMPE"
     gene            1745..>2226
                     /locus_tag="VFJ67_02175"
     CDS             1745..>2226
                     /locus_tag="VFJ67_02175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7289218.1"
                     /translation="MQRRKTMDDNDDFISCWNEILVPKWNRFRHLLSGNGAVHSNMAY
                     EYFQIKPGDKVLDIGCGYGETTLEMGRMVGPGGEVLGLDCTEAFLDVANSERDAAGLA
                     NVKFVLGDAQTYPLPENYYDVVYSRFGVMFFQNIVYALRNAHKTLKPGGKLCMIVWRT
                     "
BASE COUNT          530 a          603 c          632 g          461 t
ORIGIN      
        1 cctgcttctg cggggcggtc gaaatatccg tcacaggcga gccgctggcc atgggctact
       61 gccactgcga ctcctgccgc cactggtcgg cgggccccgt gagcacatac acgatctggg
      121 ccccgggctc ggtcgaggtc acgaagggcg ccggcaacat cggcacatac aacaaaacgg
      181 agttcagtta ccgcaagtgg tgcaagtcct gcggcggaca cctctatgcc gagcacccgt
      241 cgatcaacct caccgacgtc cccgcggata tactcccgga ccttaccttc aaacccaccg
      301 ctcacctgaa ctatcaggag accgtgctcc cgatgaagga cgggcttacc aagtacaggg
      361 acttcccgaa agaagccggc ggctccggcg agacgatgga ggagtagcga atctaacccg
      421 ttcgccctga gcgtgtcgaa gggttgaatg ggttgagctt ccttaattct tctgtcactc
      481 ctgacggttt tcatcctgca tcctgaatcg tgaaacatga atgcggcacg cgccgtttgc
      541 ttttacgccc ccttttattc tataatgata taaagctaat ggagggtagg actatgctca
      601 gaaatcagat tatttcatcc gtagccgtta ttctgacatt cggtttcttc tttctcgctt
      661 tacccgaaga aggattttcg ggactcagca cgcagataaa ttgctgccag ctggagggtc
      721 agtgcgagga taccactcag ggccccttca tgtgtaccga agaggacttc gtaccgaacg
      781 cggtttgtga tcaaggtacg ggctcgtgcg tgctgatcag cagaatcaac cctattccga
      841 ccgtgtccga atggggcttg atagccatgg cgggtattct ggggatagcc ggattcatca
      901 tcgtcagaaa aagaaaagtc gcggcttaac gaccgggcag ccctgcggct aaagcggccc
      961 gtcgaagggc caaacgggct ctgttccggt tccaggctcc cgtttttaat ctcctcagct
     1021 tctgcgaact cccgcgcact ttcatggcga tttctcgcta atagcggaat taagcgacga
     1081 aggaattctc cataaagcgg cttaattccc tgtaatcact gacaattaca ggacgcgcca
     1141 aactggcatc aggtttgcat ctacataaga taatacgaac cacaaaggag atttgacatg
     1201 agaacacata cgattacagg cagcattttc agaggaggtg ctatatgagc gagtcaaaaa
     1261 catacaaggg gacatgcttt tgcggtgcgg tggagattgc cgttacgggc gatcccgcgc
     1321 tgatggggta ctgccactgc agctcgtgcc gtcactggtc ggcggggccc gtgaacgcgt
     1381 tcaccctatg gaacccggaa gcggtgaagt acacgaaggg cggggacaac gtcctcacct
     1441 acaacaaaac ggagttcagc ttcagaaagt cgtgcaagac ctgcggcgga cacgtactga
     1501 ccgagcatcc gacgttcggc ctcacggacg tgtacgcggc gatcataccg gacctgcagt
     1561 tcaagcccgc ggtgcacgtg aactatcagg agaccgtgct tcacatgaag gatggcctgc
     1621 cgaagttcaa ggacttcccc aaggaagccg gcggctcggg cgagacgatg ccggaatagg
     1681 aaagccgatg gtaacaattt tgttattttc tttccctgca ttacggaatc ggcgataaga
     1741 aaatatgcaa aggagaaaga cgatggacga caacgatgat ttcataagct gttggaacga
     1801 aattctggtc ccgaagtgga acaggttccg ccacctgctc tccgggaacg gggccgtgca
     1861 cagcaacatg gcctacgaat atttccagat aaagcccggc gacaaggtgc tcgacatagg
     1921 ctgcgggtac ggggagacga ccttagagat gggcaggatg gtcggcccgg gcggagaagt
     1981 gctggggctc gactgcaccg aggcgttcct cgacgtcgcg aacagcgaga gggacgcggc
     2041 ggggctcgcg aacgttaaat tcgtgctcgg cgacgcgcag acatatccgc tccccgaaaa
     2101 ttactacgac gtggtttatt cgcggttcgg cgtgatgttt ttccagaata tcgtttacgc
     2161 gctcaggaac gctcacaaga cgctcaagcc cggaggcaaa ctctgcatga tcgtctggag
     2221 gacgat
//
LOCUS       DASSJH010000102         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1430976, whole genome shotgun sequence.
ACCESSION   DASSJH010000102 DASSJH010000000
VERSION     DASSJH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430976"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1431)
                     /locus_tag="VFJ67_02180"
     CDS             complement(259..1431)
                     /locus_tag="VFJ67_02180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7289219.1"
                     /translation="MNLDSSEYRAVVTVALIMATRLLGIFLILPVFSVYTERYPGSDL
                     AMAGVAFGIYALAQSILQLPFGWASDKLGRKPVLIIGILLFTAGSVYCGMADSIRELI
                     IARIIQGSGAVSSVAIASLGDVTRPQVRAQSFTIVGISIGVAFLMAIVVGPYLAAKIG
                     FSSLFYVLAGLGALSLLITLFFFPAIEKEKEEPVAARAGLWRIATMPEMRSLYTASLV
                     VSLLVNLFVFVLPLSWKALGVSDAGLWKVYLISLIPTALFVFPYIRRSEKKRALSAAT
                     KAAWGLIALSFLVYPAGAGYSLILYGAGMAYFAGHTIMQSLLPAFLTQTVGQEKRGSA
                     TGIYNLLSFFGSSLGGMLSGYLYQMSPSVPLIFGFILIILWGLTGLPEPPGEGRSI"
     gene            1529..>2694
                     /locus_tag="VFJ67_02185"
     CDS             1529..>2694
                     /locus_tag="VFJ67_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289220.1"
                     /translation="MKRARRAGNIETDSYDRELFSDLENDSQGLGELIERGSSLLPGF
                     RTLMLDLFAAFFKHNVVFLPETDVRRSALLPRRLLTGVQSDPDYAALREETVLDAFRS
                     MLAAMEMGRMILEWLRSGEGPGEKGLVREWETERAESDVEELSGESETMEEIAGREGE
                     GQEEERGEAFEKVRRGKKEELDKEKKAFTKLEDSLKKGHEGMDMKIKNMVRASMKETS
                     GSVEDAEDEITSWSASMGTGGERSAADKLDLASRLYSNEKLRRLSRIVGSLREEMLRG
                     RRKTWSRRGSEVFDVSTGSDIGRLIASELAGLANPGLRADFKKRLIEGNLLQYSLRDE
                     KGRGPMIICLDGSSSMEGKKELWSKGVCLTLLDIARRERRKFSVIVFSSGGSPSR"
BASE COUNT          620 a          709 c          862 g          503 t
ORIGIN      
        1 tgttgtcaac gttggatctg aagcccgttg tttccgtatc cgcgaggttg acgagcgcat
       61 gggatgcggc tgcgggcaga aggcacataa taataaatgt aagagccgct aattttatac
      121 taactaacat caccatcctc ctttaagtga ctttattcaa caatatcaca aataagggtg
      181 ttttttaagg gttatccggt tttttcaggg ggctgaacgg gtccggaatc cggtattttc
      241 agaatcggcg ggcccggctc agatgctccg tccttcacca ggcggctcgg ggagtcccgt
      301 aaggccccat aggattatca gaatgaagcc gaatatgaga ggcacggaag ggctcatctg
      361 gtataggtat ccggacagca tccctccgag agacgagccg aagaaagaga gaaggttgta
      421 tatgcccgtc gcggagcccc ttttctcctg tcccaccgtc tgggtcagga aagccgggag
      481 gagagactgc atgatcgtgt gcccggcgaa gtaagccatg cccgcgccgt agagtatgag
      541 cgagtagccc gcgcctgccg gatacacgag gaaagagagc gctatcaagc cccaagccgc
      601 tttagtcgcg gccgagaggg ccctcttttt ctccgagcgc cttatgtaag ggaatacgaa
      661 gagcgcggta ggtatgaggg aaatgaggta caccttccag agccctgcgt cgctcactcc
      721 gagcgccttc cacgatagcg gtaacacgaa gacgaacagg ttcacgagta gcgataccac
      781 gagggaagca gtgtagaggc tcctcatctc gggcatggtc gcgatgcgcc agaggccggc
      841 ccgcgccgcg acaggctcct ccttttcttt ttctatggcc gggaagaaaa agagcgttat
      901 caggagcgag agcgccccta atcccgccag tacatagaag aggctcgaga acccgatctt
      961 cgccgcgaga taaggcccta ccacaattgc catcaggaaa gcgactccta ttgaaatgcc
     1021 cactatcgtg aaggactgcg ccctcacctg gggccgcgtc acgtccccaa gggacgctat
     1081 cgcgaccgag ctcaccgcgc cgctcccctg tatgatgcgg gctatgatga gctctcttat
     1141 ggaatccgcc atgccgcagt aaacgctccc tgcggtaaag agaagtatgc cgataatgag
     1201 cacaggcttc cttccgagct tgtcgctcgc ccagccgaag gggagctgga gtatgctctg
     1261 cgcaagcgcg tatatgccga aggcgacccc ggccatcgcg aggtccgagc ccgggtatct
     1321 ctccgtgtac acgctgaaaa cgggcagtat gaggaatatg cccaggagtc tcgtggccat
     1381 gatgagggca accgtcacga cggccctgta ttcgcttgaa tcgaggttca tttgttcaat
     1441 ttcacgcttg tcacggaggg ggttttttat tttaacacgg gtttccgaaa ttgatacacc
     1501 cggaggggcg tgttagcttt atttcaccat gaaacgggcg cgccgggcgg gaaatataga
     1561 aacggactcc tacgacaggg agctcttctc ggatctcgaa aacgattcgc aaggtcttgg
     1621 agagctcatt gagcggggct cctctctcct ccccggcttc cgcacgctca tgctcgacct
     1681 ctttgccgcg ttcttcaagc ataacgtggt attccttccc gagacggacg tgagaagaag
     1741 cgcgctgctc ccgcggaggc ttctgacggg ggtgcagagc gaccccgatt acgcggcgct
     1801 ccgtgaggag accgtgctcg atgccttcag gtcgatgctc gccgccatgg agatggggag
     1861 gatgatcctc gagtggctga ggtccgggga aggcccggga gagaagggcc tagtgaggga
     1921 atgggagacg gagagggccg agagcgatgt ggaggagctt tccggtgaga gcgagacgat
     1981 ggaagagatc gccggccggg aaggggaggg tcaggaagag gagagaggcg aggcgttcga
     2041 gaaggtgagg cgcgggaaga aagaagagct cgataaggaa aagaaggcgt ttacaaagct
     2101 cgaggacagc ctcaaaaagg gtcacgaggg catggacatg aagataaaga acatggtaag
     2161 agcctccatg aaggaaacct cgggcagcgt cgaggacgcg gaggacgaga taacgtcctg
     2221 gagcgcatct atgggcacgg gcggcgaaag atcggccgcc gacaagctcg acctcgcctc
     2281 acggctatat tcgaacgaaa agctgagacg cctctcccgc atcgtgggca gcctcaggga
     2341 agagatgctc cgggggagga ggaagacctg gtcgaggcgg gggagcgagg tattcgacgt
     2401 ctcgaccggg agcgacatag gcaggctcat agcgtccgag ctggcaggtc tcgcgaaccc
     2461 ggggctccgc gcggatttca agaagcggct catcgaaggg aacctgctcc agtactcgct
     2521 cagggacgag aagggcaggg gcccgatgat catctgcctc gacggaagct cgtcgatgga
     2581 gggtaagaag gagctctggt cgaaaggcgt gtgcctcact ctcctcgaca tcgcccggag
     2641 ggagcgtagg aagttcagcg tgatcgtgtt ctcgtcggga ggctcgcctt cccg
//
LOCUS       DASSJH010000103         4651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_996437, whole genome shotgun sequence.
ACCESSION   DASSJH010000103 DASSJH010000000
VERSION     DASSJH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996437"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /gene="pyrR"
                     /locus_tag="VFJ67_02190"
     CDS             complement(<1..370)
                     /gene="pyrR"
                     /locus_tag="VFJ67_02190"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET7289221.1"
                     /translation="MPTRTVMDSDSVNRTISRIVNQILERNNGPRDLAIIGIRTRGVY
                     IAERIAGEIEKLEGIRPPMGILDISFYRDDLMRKTEWPNVKKTEIPFPVEDKKVILAD
                     DVIFTGRTTRAAIDEIMDYGR"
     gene            complement(383..733)
                     /locus_tag="VFJ67_02195"
     CDS             complement(383..733)
                     /locus_tag="VFJ67_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289222.1"
                     /translation="MKSLSSVFWILLLALIFYVGFKIVPIYYRGVFGIRGVCQENADV
                     YHKYGRVYVQNGVSEMLQNIGIPKEKSQYRIKEEGDKIIISITYKDSATFFDRHTQEF
                     EFDYECEGVRKAVY"
     gene            complement(775..1710)
                     /gene="lepB"
                     /locus_tag="VFJ67_02200"
     CDS             complement(775..1710)
                     /gene="lepB"
                     /locus_tag="VFJ67_02200"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7289223.1"
                     /translation="MFGPWSKEAKARNRAKQIISQTQALLNKNSSKISAESAAVVSKR
                     IAGAEMALAGGDTEEILRKTSDLEGASRDYLSRFAKSKLRQNIEALVFAVGLALLIRT
                     FVFQPFKIPSGSMIPTLLVGDHLLVSKFVYGTKIPFTDKVVLPVEQIKRGDVIVFTYP
                     NTEHDPSKNGVYYIKRVIGVPGDSIDLDGRQLVINGQKVPLEYTGSYTDERNGEQFDE
                     YNEDLFGEKHRVIFRKGRDSTNKGSFIPVSAVPEGYVFVMGDNRDNSQDSRFWGFVPE
                     GNIAGKAFLIHWSWDFDNPDIGGKVRWDRILSGID"
     gene            complement(1807..3969)
                     /locus_tag="VFJ67_02205"
     CDS             complement(1807..3969)
                     /locus_tag="VFJ67_02205"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET7289224.1"
                     /translation="MIRLNDITERVNSYLYLSDTDVEVLKKAYVYSAKVHAGQTRSSG
                     EPYLSHPLEVCGILAEMKLDMSSIVTGLLHDTVEDTLATTEEIDTLFGSEIAFLVDGV
                     TKISQLPYTTKIERQAEGFRKLILATAKDIRVVLIKLADRLHNMRTLEHLPEGRRKSI
                     AKETFDIYAPLAHRLGINWLANELEDLSFKFLNPEEYDRLSASIEERKKEWSAHVDEI
                     KKIINGKLEEFDINAEVTGRFKHVYGIYRKMETQNLEFESVYDVIAFRIITNSLRECY
                     EALGAVHSAWKPVPGRFKDYIALPKGNGYQSLHTTVIGPFGERMEIQIRTRAMHEVAE
                     YGVASHWKYKEGKLDSHDTYEIYSTLRQLLEWKDIKDPTEYMEAIKGELISDVVYVFT
                     PQGDLMELPSGATPVDFAYAVHSEVGNTCTRANVNGKLVPLNHQLRTGDTVEIVTSAD
                     RHPNRDWLKFVVTSRAKTKIRSWLRNEERTQSEIVGKSICERKFKQHGLDFQSMLRSG
                     ELQSALSDLKFRDEKELYLAVGFGKISATDLLKRVLPTDRMHQGPEKESRIEKIIQTI
                     TGSSEGGVLISGYNDVMIRFANCCSPLPGEQITGYITRGKGITIHKNDCPRLLDVDDA
                     RRIDVEWDKNFKPQRPARIVVMCADRPGMLSNITSSFSSSDINISKAEIHSTDVDTAI
                     GTFDVVVSDLEQLENVMKSIKQVDGVRSVERMLEWERA"
     gene            complement(3974..4582)
                     /gene="gmk"
                     /locus_tag="VFJ67_02210"
     CDS             complement(3974..4582)
                     /gene="gmk"
                     /locus_tag="VFJ67_02210"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7289225.1"
                     /translation="MREGNIFVVSGPSGGGKTTLVRKVLEGLNDLRFSVSCTTRKPRA
                     DEVNGKDYIFVSDDEFGKMVGEGKFAEYAKVHDHMYGTPLSELENAAKSEVDLILDID
                     VQGARQIRQRFGSGVYCFVLPSSFEILKSRLMERGSEDKIEMEKRLSDARQEMEDLGN
                     YDYIIINDDLDRAAECLSSVIISTRCGREKSIGIIKKGYSPG"
BASE COUNT          897 a         1336 c         1152 g         1266 t
ORIGIN      
        1 gtctaccgta atccattatc tcgtctatgg cagccctcgt cgtgcgtccc gtgaagatta
       61 cgtcgtctgc gaggataacc ttcttgtcct cgacagggaa tgggatttca gttttcttga
      121 cgttaggcca ttccgttttt ctcatgaggt cgtctctgta gaacgatata tcgagaattc
      181 ccatcggcgg tcttatccct tcgagcttct cgatctcgcc ggcgatcctc tccgctatgt
      241 agacgcccct agtcctgata ccgataatcg caaggtcgcg ggggccgtta ttcctctcga
      301 gtatctggtt tactatcctc gatatggtcc tgttgaccga gtccgaatcc attactgttc
      361 ttgtaggcat aaatgttctc gctcaatata cagccttgcg cactccctcg cactcgtagt
      421 cgaactcgaa ctcctgtgtg tgcctgtcga aaaaagtcgc cgaatcttta tatgtgatcg
      481 agataatgat tttatcgcct tcttctttga tgcggtattg gcttttttct ttcggtattc
      541 cgatattctg aagcatctcc gacaccccgt tctgcacgta gacccgccca tatttatggt
      601 aaacgtcggc gttctcctgg catacgcccc ttatgccgaa tacgccgcgg tagtagatcg
      661 gcacgatttt aaatccgacg tagaatatca gggcaaggag taagatccag aataccgagg
      721 ataagctttt catcagccgc ctcctgcaat cccgtcattc ggccgaaagg ccggctagtc
      781 tataccggag agaatcctgt cccatctcac ctttccgcct atatccgggt tatcgaagtc
      841 ccatgaccag tgtatcagaa aagccttccc ggcgatattg ccttcgggca caaaacccca
      901 aaaccttgag tcctggctgt tgtcccggtt atcgcccatg acgaacacgt agccttcggg
      961 caccgcgctt acggggataa agcttccttt gttggtcgag tccctgccct tcctgaaaat
     1021 aacgcggtgt ttctcgccga agaggtcttc gttgtactcg tcgaactgct ccccgttcct
     1081 ctcgtccgta taagagcccg tatactcgag cggcaccttc tgcccgttaa tcacgagctg
     1141 cctcccgtcg aggtcgatgc tgtctccggg aacgccgatg acgcgtttta tataatatac
     1201 gccgttcttg gaagggtcgt gctccgtatt ggggtatgtg aagactatta cgtcccccct
     1261 ctttatctgc tcaacgggca gtactacttt atccgtaaat ggtatcttcg tcccatatac
     1321 gaacttgctt acgagcaggt ggtccccgac gaggagcgtg ggtatcatgg agccagaggg
     1381 tattttaaag ggctggaata cgaatgtcct gatcaggagc gcgagaccca cagcgaacac
     1441 gagcgcctct atgttctgtc tcagcttcga tttcgcgaac ctggaaagat agtctctcga
     1501 tgcgccttcg agatcgctcg tcttgcgcag tatctcttcc gtatctccgc cggcgagcgc
     1561 catctcggcc cccgctatcc tcttgcttac gaccgccgcc gattccgcgc ttatcttcga
     1621 gctgttcttg tttaaaagtg cctgtgtctg ggaaattatc tgcttggctc tatttctggc
     1681 ttttgcctct ttggaccatg gaccgaacat aattgaggtt tgagtatata tggtgaatcg
     1741 gctcgtttca atattttcgt caggccggtt catatccggg gcggcataaa gaattctgtc
     1801 attatatcag gccctttccc attcgagcat cctttcgacc gacctcactc cgtcgacctg
     1861 ctttatggac ttcatcacgt tctcgagctg ctcgaggtcg ctcaccacga catcgaaagt
     1921 cccgatggcc gtgtctacat cggtcgaatg tatctcggct ttcgagatgt tgatgtccga
     1981 agaggagaac gagctcgtta tgttggagag catgcccgga cggtcggcgc acattacgac
     2041 tatcctcgcg ggacgctggg gtttgaaatt cttgtcccac tcgacgtcta tgcgccgcgc
     2101 gtcgtccacg tcgagtagac gcgggcagtc gttcttgtgg atagtgatgc cctttcccct
     2161 cgtgatgtag cccgtgatct gttcgcccgg gagcggcgag cagcagttgg cgaaccttat
     2221 catgacgtcg ttgtagccgc ttatgagcac cccgccttcg ctcgagcccg taatggtctg
     2281 tatgatcttc tcgatcctcg actctttctc cggtccctgg tgcatcctgt cggtcggcag
     2341 tacccgtttg agcaggtcgg tcgccgaaat cttgccgaac ccgaccgcga gatagagctc
     2401 cttctcgtcc ctgaatttaa ggtccgaaag cgcgctctgg agctccccgc tcctgagcat
     2461 cgactggaag tcgagcccgt gctgcttgaa cttccgctcg caaatggatt tacccactat
     2521 ctcggactgc gtcctctcct cgttcctgag ccacgagcga atcttcgttt tggcccggga
     2581 agtgactacg aacttgagcc agtccctgtt agggtgcctg tcggccgaag tgactatctc
     2641 gaccgtatcg ccagtcctca actggtggtt gagcgggacg agcttaccgt tgacgtttgc
     2701 cctcgtgcat gtgttcccga cctcggaatg cacggcgtag gcgaaatcca caggggtcgc
     2761 tccgctcggg agctccatca ggtccccctg gggggtgaag acgtaaacga cgtccgagat
     2821 gagctctccc ttgatcgcct ccatgtactc ggtcgggtcc tttatgtcct tccattcgag
     2881 gagctgtctc agcgtggagt agatctcgta cgtgtcgtga ctgtcgagct tgccttcctt
     2941 gtatttccag tgggatgcta cgccgtattc ggcgacttcg tgcatcgcgc gcgtccttat
     3001 ctgtatctcc atcctttcgc cgaaaggccc gatcacagtc gtgtggaggg attggtagcc
     3061 gttccccttg gggagagcga tgtaatcctt aaagcgtccc ggtacgggct tccacgccga
     3121 gtgcacagcg cccagggcct cgtaacattc ccttaaggag ttggtgatga tcctgaacgc
     3181 tatgacgtcg tacacgctct cgaactctaa gttctgagtc tccatcttcc tgtaaatacc
     3241 gtaaacgtgc ttgaacctgc cggtgacctc ggcgtttatg tcgaactcct cgagcttgcc
     3301 gttgattatc ttcttaatct cgtcgacgtg cgccgaccat tccttcttcc tctcctctat
     3361 cgacgcggag agcctgtcgt attcctccgg gttgaggaac ttgaacgaca ggtcctcgag
     3421 ctcgtttgca agccagttta tacccagcct gtgtgcgagc ggcgcgtata tgtcgaacgt
     3481 ctccttcgct atgctcttcc gcctgccctc gggcaggtgc tcgagcgtgc gcatgttatg
     3541 gagcctgtcg gcgagcttta tgaggaccac ccttatgtcc ttggcggtcg cgagaatgag
     3601 cttcctgaat ccctcggcct gccgctcgat cttggtcgta taggggagct ggcttatctt
     3661 cgttaccccg tccacaagga atgcgatctc gcttccgaag agcgtgtcta tctcctcggt
     3721 agtggcgagc gtgtcctcga ccgtatcgtg gaggagaccc gttacaatgg acgacatgtc
     3781 gagcttcatc tcggcaagga tcccgcatac ttcgagcgga tggctgagat aaggctctcc
     3841 cgagctcctc gtctggcctg cgtggacctt ggccgaataa acgtaagcct ttttcaggac
     3901 ctcgacgtcc gtatcagaca ggtagaggta cgaattgacg cgctcggtta tgtcgttaag
     3961 ccttatcatt gtcttagccg ggcgaatacc ctttttttat gattcctatg gacttttccc
     4021 tgccgcacct ggttgaaatt attacggaag aaagacattc ggcggcccgg tcaaggtcgt
     4081 catttataat aatataatca tagttgccca ggtcttccat ctcctgcctg gcatcggaga
     4141 gccgcttttc catctcgatc ttgtcctcgc tccctctttc catgagccta gactttaaaa
     4201 tctcgaacga cgacggcagg acaaagcagt aaactcccga accgaacctc tgacggatct
     4261 ggcgcgcccc ctgtacgtct atatcgagta taaggtcgac ctccgacttt gcagcgtttt
     4321 cgagctcaga aagaggcgtc ccgtacatgt ggtcgtgtac cttcgcgtat tcggcgaatt
     4381 taccctcccc gaccatcttc ccaaactcgt cgtcggagac aaaaatatag tcttttccat
     4441 tcacttcgtc tgccctcggc tttcttgtgg tgcaggaaac agaaaatctg aggtcattca
     4501 gaccttcgag cacctttctc actagggtcg tctttccgcc ccccgacggt cccgaaacta
     4561 cgaagatatt cccttctcgc atggcgttta tcccttttgc tcaacaaata tcgagtttat
     4621 atacaatttt aggacaagta acaataaata t
//
LOCUS       DASSJH010000104         4107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_996538, whole genome shotgun sequence.
ACCESSION   DASSJH010000104 DASSJH010000000
VERSION     DASSJH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996538"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFJ67_02215"
     CDS             <1..627
                     /locus_tag="VFJ67_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7289226.1"
                     /translation="LFIQTFESGNVAPGQIVGCNTPVDADSNDDCFSPGDILPGIAFS
                     TDPDFGGTILSGPFLFSNLNPANILLPDGSAQKSVITFPDNSVRAVGLELGCLFDQGV
                     GGPCTGQTMLVQVYGAGNALLGETIVPVDSLFETFVGINTDEQIERITFIDADLSVLS
                     FNAVEKVYFPNEGLHNIPTISEYGAAALALALGLAAAYAVRRKRASCA"
     gene            734..1483
                     /locus_tag="VFJ67_02220"
     CDS             734..1483
                     /locus_tag="VFJ67_02220"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7289227.1"
                     /translation="MGRLDGKTALITGANSGIGFASARRFIAEGARVAITGRRADAVE
                     KAARELGPNASGIVADAGNLGDIEQLIKKVKQLYGSLDILFLNAGTAPIATIEQQDEA
                     SFEELFNVHVKGPYFTVQKALPLLKEGSSIIFTTSIGNVKGFSGVSVYSAAKAALRSF
                     TRTLAAELAPKGIRVNSLSPGPIETPLYGKTGLSEKEIDEFQKNIISMVPLGRFGTAD
                     EIAAVAVFLASGDSSYITGTEITADGGTAQV"
     gene            1588..2325
                     /locus_tag="VFJ67_02225"
     CDS             1588..2325
                     /locus_tag="VFJ67_02225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7289228.1"
                     /translation="MPGFHHPLRNGHSQEEVTKIFRRHWDIYRKVVEHDCMSHKAAYG
                     RLRDIINDEINRPFSFADLACGDAYWSSRCLAETDVDEYTGIDLSEWALELAEKELDK
                     VRAPHRLITGDFEEFDRYIDSPPDVVWVGLSVHHLATDEKERFMMKVREALPPGGAFL
                     IYEPTFIEGEDGTTYLSRFEDVVRRNWTGLTPEELETVFDHVRKSDIPELPSKWISLG
                     RDAGFSTAEEVYTDPTDLYTVFKYGAT"
     gene            2892..3164
                     /locus_tag="VFJ67_02230"
     CDS             2892..3164
                     /locus_tag="VFJ67_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289229.1"
                     /translation="MKKLILTILGVLMLSPALSMAVEESDSVIAYSTYPNDVISYIYV
                     IPAEKKDAESGQSPEITYLKPPVFTNQLFAGRNYKTVLCGKYTICN"
     gene            complement(3263..3706)
                     /locus_tag="VFJ67_02235"
     CDS             complement(3263..3706)
                     /locus_tag="VFJ67_02235"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HET7289230.1"
                     /translation="MNTAMKYALVLVMSALPLVSAKAEEAVLNVTPGNYLIKRETSSD
                     ADPNAIKKDEEKCITSPVFNPVSALPPNGGCTASNVKKSGNSVTFNITCTGGPEMPPL
                     TGTAEYSSNGMAIGWNIVLKGDIEGKPMTIVNKAEGKRIGDCKPQ"
     gene            complement(3811..>4107)
                     /locus_tag="VFJ67_02240"
     CDS             complement(3811..>4107)
                     /locus_tag="VFJ67_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289231.1"
                     /translation="EKKQKSTDRTEFTKNLVLKLKHFNSSHTPEIFFHASTHDFLNVN
                     SVMSEENQDCRAPLAKTGIVRYTPTRPLPLGKGEGNINKDGIATIPYISNPSGA"
BASE COUNT          974 a         1166 c         1036 g          931 t
ORIGIN      
        1 ttatttattc agacgttcga aagcggcaac gtcgctccgg gacagatagt gggatgcaat
       61 accccggttg acgccgattc aaacgacgat tgcttttcgc ctggcgacat tctgcccgga
      121 atcgctttca gcacagaccc cgatttcggc ggaaccatac tctccgggcc ttttcttttc
      181 tcgaacctga accccgccaa tatactcttg cccgacggct cggcgcagaa gagcgtaatc
      241 accttccccg acaatagcgt aagggcggtg gggcttgagc tcggctgcct gttcgatcag
      301 ggcgttggcg ggccctgcac cgggcagacc atgctcgtcc aggtttacgg ggcaggaaat
      361 gcgcttctcg gcgagacgat cgtacccgtc gacagcctgt tcgaaacgtt cgtaggtata
      421 aacacggacg agcagataga gaggatcacg ttcatcgacg cagacctcag cgtgctcagc
      481 ttcaacgcgg tcgagaaggt gtatttcccg aacgagggct tacacaatat cccgaccata
      541 tcagagtacg gcgcagccgc gctggcgctc gctctcggcc tcgccgctgc atacgcagtc
      601 cgaaggaaac gtgcgtcctg cgcctgagcc ccctcaaccg gatatcgttc cccagcaagc
      661 ttccccccga atccttttac gcctgggatg ctactaaaca tatatctaca tcagttccga
      721 aaggagacga attatgggca gactggacgg aaagacagcg ttgataacgg gcgcgaacag
      781 cggcataggc ttcgcgtcgg caagacgctt tatcgccgaa ggcgcaaggg tggcgatcac
      841 cgggagacgc gccgacgcgg tggagaaagc agcgcgcgag ctgggcccga acgcatcggg
      901 cattgtcgcc gacgctggga atctcggaga tatcgagcaa ctcattaaaa aagtaaaaca
      961 gctctacggg agcctggaca tcctgttcct taacgcaggc acagcgccta tcgcaacaat
     1021 agagcagcag gacgaggctt cattcgaaga gctgttcaat gtccacgtca aagggccgta
     1081 tttcacggta caaaaagccc tcccgctcct gaaagagggc tcgtcgataa tcttcacgac
     1141 ctcgatcggc aacgtgaaag ggttttccgg cgtaagtgtc tattccgctg caaaagcggc
     1201 gctccgctct ttcacgcgca cgctcgcggc cgagctcgct ccgaaaggga tacgcgttaa
     1261 ttcgctcagt cccggtccga tagaaacgcc gctctacggc aaaacgggcc tctcggagaa
     1321 agaaatagac gagtttcaga agaatatcat ttccatggtg cccctcggcc gtttcggcac
     1381 ggcggacgaa atcgccgcag tggcggtgtt cctcgcctcg ggcgattcct catatatcac
     1441 aggtacggaa atcacggctg acggcggcac ggcgcaggtc tagatgaccg atgacccgca
     1501 accctccggt gacgggacga attgtcccct tcccctccac cgcgtcaggc agtatcctta
     1561 cgggaaattt cgcacggagg ctcgctaatg ccgggattcc atcaccctct cagaaacgga
     1621 cactctcagg aagaggtaac caaaatattc cgccgtcact gggacatata cagaaaagtc
     1681 gtcgagcacg actgcatgtc ccacaaggcc gcatacggca ggctccgcga tataatcaac
     1741 gatgaaatta accgcccgtt ctcctttgcc gacctcgcct gcggcgacgc gtactggtcc
     1801 tcccgctgcc tcgccgaaac ggacgtcgat gaatacacgg gcatcgacct ctccgaatgg
     1861 gcgctcgagc tcgcggagaa ggagttagac aaggtccgcg cgccgcaccg cctcatcacg
     1921 ggagacttcg aggaattcga tagatacatc gattctcctc cggacgtcgt gtgggtcggc
     1981 ctgtccgtac accaccttgc gacggacgag aaggaaaggt tcatgatgaa ggtgagggaa
     2041 gcactccccc caggcggagc cttccttatc tacgagccta cgttcatcga gggcgaggac
     2101 gggacgactt accttagcag gttcgaggac gtcgtcagac gcaactggac cgggctcacg
     2161 cccgaagagc tggaaacggt cttcgaccac gtgaggaaat cggacatccc cgaactgccg
     2221 tccaagtgga tcagcctcgg cagagacgcc ggtttcagca cggcggaaga ggtctatacc
     2281 gacccgaccg acctctacac cgtgttcaaa tacggcgcta cctgactgtt tgcgccggct
     2341 aacccaatcc gtccgtcctg cttgtcaggg cgtagccttg acgaaaccgg aagcttctcg
     2401 aaattcagag tattttcgac attcacggaa tcgacagccc aacacatata ttgcccgctg
     2461 taatatagcg ggcaaaaggg acattacggg atttgcgtca agaaaagcgg gcccgcgccc
     2521 gcgggcgttc gatctctaac caccacttct gccaaatatg taccgtgagc gacttaagcc
     2581 cctgttgtca attagcaacc gtaaaatgga cactatgtgt ccagcgtaaa aaaatctttc
     2641 ggcttcccat ttgaaagatt ttcgcttaaa acacaagatt tttagcgaac ttcccgtagt
     2701 ccccaaaaga tttttgctac ttttgatctc tcaaaagtag aaacttgaat ctttttataa
     2761 accacactac gtatgtctca tcaggacagc ctgaatcctg acactttttt gaatcttttt
     2821 acaatttccg ggcgtctaaa attaacagat attgacaatt aagggaaacg ggtatttctt
     2881 taggttgggt tatgaaaaag ttaattctca cgatattagg ggttcttatg ctgtcaccgg
     2941 cgctgtccat ggctgtggaa gagtcagaca gcgtgatagc atattccacc tatccgaacg
     3001 acgtcatctc gtacatctac gttatccccg ccgagaagaa ggacgcggaa tcaggccaga
     3061 gcccggagat cacgtatctg aagccgcctg tattcacgaa ccagctcttc gcgggcagga
     3121 attacaagac agtcctctgc ggcaaataca cgatctgcaa ctgaatccgg gcagacatac
     3181 ttaataaatg ggttcagatt actgccaata ttactcggta ataccgccga acattctcaa
     3241 ctttacaacc gcacacacac gatcactggg gcttacagtc cccgatcctc ttcccctcgg
     3301 ctttatttac gatggtcatc ggcttcccct ctatatcccc tttgagcact atgttccatc
     3361 cgatcgccat cccgttcgag ctgtactccg ctgttccggt caacggcggc atctcgggtc
     3421 cgcccgtgca ggtgatattg aacgtcaccg aattccccga cttcttcacg ttgctggcgg
     3481 tacatcctcc gttgggcgga agcgcactca cagggttgaa caccggactc gtaatgcatt
     3541 tctcctcgtc cttctttatg gcgttcggat ccgcgtccga gctcgtttct ctctttatga
     3601 ggtaattccc cggcgtcacg ttcagcacag cctcctccgc cttcgcgctt acgagcggca
     3661 gagcggacat aaccaaaacc agagcatact tcattgcggt attcatataa gaacttcctc
     3721 cgttgtcggc tttatcgaga tgaaaaatta tagtgcacga aatgccttcc ttcaaatata
     3781 cccgttatta tccccgcccg gactataaat ttacgccccc gagggattag aaatataggg
     3841 aatcgtggct atcccgtctt tgtttatgtt cccctccccc tttccaaggg ggaggggacg
     3901 ggtgggggtg taccttacta ttcctgtctt tgcgagcgga gcgcggcaat cttgattttc
     3961 ttccgacatt acagaattta cgttaagaaa atcgtgtgtt gaagcgtgaa aaaatatttc
     4021 aggggtgtgg gaggaattga aatgttttag cttcaacaca agatttttag taaattctgt
     4081 tctgtcggtt gatttttgct tcttttc
//
LOCUS       DASSJH010000105         7215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1104673, whole genome shotgun sequence.
ACCESSION   DASSJH010000105 DASSJH010000000
VERSION     DASSJH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104673"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFJ67_02245"
     CDS             <1..778
                     /locus_tag="VFJ67_02245"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HET7289232.1"
                     /translation="VSYFVKIADGLGFDMPGDLLELLNPNKFNLTENKNFENKNFYDW
                     YNTTKAGWKEIISIAQHYGVKTRYLDFTFNPHTALFFAAMEVTKKYQRECDKIPESNN
                     QFSLWVLDKDYLNNPGCTINHFEVPTARNKYLNAQKGLFLSPPLNQLYEPGFYGRRTE
                     SVNKKSLDILEVIKDNNYHLVSNGDIELQRKWPIFYKFSFPFPSAPKILQVLDDEYKI
                     NMANQKPNLENIIPYRKFREKLDELASELEDNKGNSDRSP"
     gene            958..3006
                     /locus_tag="VFJ67_02250"
     CDS             958..3006
                     /locus_tag="VFJ67_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumatin family protein"
                     /protein_id="HET7289233.1"
                     /translation="MRFPEFTRLGSLIVLASFMALLISAGGGKFAQAQNATITINNYC
                     SETIWVAAVPGITSGTISGGSNAGAISTLGGWELDSNATATVTVPGSYSGRFWGRTGC
                     SFDSSTNVCDPQTVNVNGNNYVIANCCDTGGCMNGSNFALDCAQTGLPPATLAEFTLA
                     SAGGGLDNYDVSMVDGGNVAVEIIPSSSDYDCSTNGNCIFTGNLPGTNSPTCTQDSDC
                     YPLFGFGYKWKCDPTLNECVNPFFCGSPGCTDTAGCAPSGLVQSDLPSSPWTGSSGFA
                     VSQANCPSELILNNDQNQGSTYVGCFAPQKFCRQACSQDGDCGPPYTFNCGTSGYCEQ
                     SKADPVILGADCDTTIDNTTNGNLWACSGTNSGSCFTAGTNANCCGCPPWAPGYPGGA
                     CLAGNNQDWQSVAEPISAIFNTSSPTSYAFPYDDAIKLFNCQNSSNQAVNYTVNFCPN
                     NTDGDAVQNSNDADVDNDGVMNSVESGNAATTANAEGDLVGTANDGDADGIPNRRDLD
                     SDNDGLTDLYECGGTDDADKDGEVDSDTDANLNGLADEYDLAQGGNMLKCADTDGDGI
                     RDFLDINSDNQGGTDYEENGGTDTDGDGLPDTTEDANADGLLDVFDSQRGGAPLEIRD
                     SDGDGIPDQLDPEKSGDNGGSCALAPAGSDTSDAALLPLLLLPALILFRRSLRIYSKD
                     "
     gene            3524..4807
                     /locus_tag="VFJ67_02255"
     CDS             3524..4807
                     /locus_tag="VFJ67_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7289234.1"
                     /translation="MQAQETRLLPHKKTVIASSLAIILEWYDFSIFGFYAVVLSEIFF
                     PFTDETTAILSTFMIFALSFLFRPLGSVVISSLGDRFGRKNAFLFTVILMTFPTVLIG
                     LLPGYGKMGIAATVLLIILRIAQALSVGGERSATLSLLAEIAPPRLRGLYGSMSLFST
                     TAGILLASFVCGLVSTALDHDELAGWGWRIPFLLGAVSGLAAYSLRKTVSESAMFLDL
                     KNSGNTSKSPVRESLTGHWKPVLIIFAATIMFAVSFYLIFVYIPTFATRYGGMTLAQI
                     LNINTMNLGLCTMLVPLSGYFSDRVGRKPAIITGCAGEVVMGFLLFKVFAGNDITLKI
                     IVQFGAGLMNVIFAGAFAAYMVESFPTRVRMSGISMGNVIGFSIFGGSAPLIASYLID
                     RTGDVTAPGIYLAAASAVSLIAVLFAKETYKSELR"
     gene            complement(5333..6127)
                     /locus_tag="VFJ67_02260"
     CDS             complement(5333..6127)
                     /locus_tag="VFJ67_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET7289235.1"
                     /translation="MLEKLKYRIHDVMEPDDTSPVAEQIFVLSIMGLIVLNVIAVILE
                     TVDSIEARFGAFFNVFNDVSIVIFTVEYLLRLWTCTVDERYRHPVTGRIKYIFSAFAL
                     VDLIAILPFYLPFVTTVDLRFLRVLRLLTLLKMTRYADEFKLFGNVLRAKRREIVISA
                     ILVLMLIILSSSLMYYIEHDKQPEAFSSIPAAMWWAVVTLSTVGYGDVLPLSPLGKLV
                     AACVSMLGIALFAIPAGIIASGFVEEMRKKESVVKICPHCGKRIGE"
     gene            6243..6713
                     /locus_tag="VFJ67_02265"
     CDS             6243..6713
                     /locus_tag="VFJ67_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7289236.1"
                     /translation="MKTRLAHVSITVKDLDRLAEFYRKALGFTEVRPEKGFSGAWLEK
                     GTGVPGASIRRVHLRLPGSSPDVALLELIEYAGKAEDKSPPAADSTGLRHIAIETGSA
                     SELARLRDLVIENGGSGLGDISENRIDGLGTVTFIYMTDPEGNIVELLNWRESL"
     gene            complement(6757..>7215)
                     /locus_tag="VFJ67_02270"
     CDS             complement(6757..>7215)
                     /locus_tag="VFJ67_02270"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HET7289237.1"
                     /translation="KLVFSNQRRHLGYCKPRRKIISLSLAFMNANPYPVMKDTLLHEI
                     AHAIHYIETGKTNHDNGWREVARRVGCRPERCAPVDGLIMPRGKYVGVCPVCRKAVHF
                     YRKVRRSYSCNDCTNGYDSKYRLRMMTTDKYEMNGGKLRTPVAYKKARSK"
BASE COUNT         1703 a         2048 c         1858 g         1606 t
ORIGIN      
        1 tgtttcatat ttcgtaaaga tagctgatgg actaggattc gatatgcccg gcgatttatt
       61 agaattattg aatccgaata aatttaatct caccgaaaat aagaacttcg aaaataagaa
      121 cttttatgat tggtataata ctactaaggc tggctggaaa gaaattatat ctattgctca
      181 acactatggt gtcaagacca gatatttaga ttttactttt aatcctcata cagctctttt
      241 ttttgcggcc atggaagtca caaaaaagta tcaacgcgaa tgtgacaaaa ttcctgagtc
      301 aaataatcaa ttttctttat gggttttgga caaagactat ttaaataacc ccggctgcac
      361 gataaatcat tttgaagtgc caacagctag gaataaatat ttaaatgcac aaaagggtct
      421 atttttatca cctcccctaa atcagttata tgaaccgggg ttctatggtc gtagaacaga
      481 aagtgtaaat aagaaatctc tcgatatatt agaagtaatt aaagataata actatcactt
      541 agtaagtaat ggtgacatag aattacaaag aaaatggcca attttttata aattcagctt
      601 tcctttccct tcagcaccaa aaatcttaca agtattagac gacgagtaca aaatcaatat
      661 ggcaaaccaa aaaccaaatt tagaaaatat cattccatat agaaaattca gggaaaagtt
      721 agatgagctg gcttctgaac tagaggataa taaaggaaac tctgacagaa gtccctaatt
      781 gattcagtac attcccccct tcctcccggt agaaatcggc actattatta gtcagcccat
      841 tgagggggat actcgggcgg tgatttacgc tgtacttcat ccgtccgctt cgctccctac
      901 gtacttatgt taaaggatat ttacaccggc ggtagaaaat ctaaaggagg atttcacatg
      961 cgtttcccgg aatttacaag gttaggctcg ctcatcgttc tcgcttcgtt catggcgctt
     1021 ctcataagcg cgggcggtgg caagttcgcc caagcccaga acgcgaccat caccatcaac
     1081 aactactgct cggagacgat ctgggtcgcg gcagtccccg gcatcacgtc gggaaccata
     1141 tcgggcggga gcaatgccgg cgcgatcagc acgctcggcg gctgggagct cgactcgaac
     1201 gcgacggcca cggtcaccgt gcccggcagc tattcgggca ggttctgggg tcggacgggc
     1261 tgcagcttcg attcatcgac caatgtgtgc gacccgcaga cggtaaacgt caatggcaac
     1321 aattacgtca tagccaactg ctgcgacacg ggcgggtgca tgaacgggag caacttcgcg
     1381 ctcgactgcg cgcagacggg cctgcccccg gcgactctcg ccgagttcac cctcgcttca
     1441 gcgggcggag gactcgacaa ttacgacgtc agcatggtgg acggcggcaa tgtcgcggtc
     1501 gaaataatcc cgagctcctc agactacgac tgctccacga acggcaactg catattcaca
     1561 ggcaatcttc ctggcacgaa ctcccccacc tgcacgcagg attccgactg ctacccgctc
     1621 ttcggcttcg gctacaagtg gaagtgcgac ccgaccctga acgagtgcgt gaacccgttc
     1681 ttctgcggga gccccggctg cactgacacc gccggatgcg cgcccagcgg actcgtgcag
     1741 tccgaccttc cctcatcccc ctggacgggc tcctcgggtt tcgcggtctc tcaggcgaac
     1801 tgcccctcag agctcatatt gaataacgat cagaaccagg gctcgaccta cgtcggctgt
     1861 ttcgcgcccc agaaattctg caggcaggca tgctcacagg acggcgactg cggacctccg
     1921 tacacgttca actgcgggac gtccggatac tgcgagcagt cgaaggccga ccccgtcata
     1981 ctcggggccg actgcgacac cactatagac aatactacaa acgggaacct ctgggcgtgc
     2041 tcggggacga actcgggctc gtgtttcacc gccgggacga acgccaactg ctgcggatgc
     2101 ccgccgtggg cgcccggata tcctggcggg gcatgcctcg ccggcaataa ccaggattgg
     2161 cagtcagtgg ccgagccgat ttcagcgatc ttcaacacct cgagccccac ttcatacgcg
     2221 ttcccttacg acgacgctat aaagctcttt aactgccaga actcctccaa ccaggccgtc
     2281 aactatacgg tcaacttctg ccccaataat acagacggcg acgcggtgca gaactctaac
     2341 gacgcggacg tggacaacga cggcgtcatg aacagcgtcg agtccggtaa cgccgcaacc
     2401 acggcgaacg ccgagggcga tctcgtaggc acggccaacg acggagacgc ggacgggatt
     2461 cccaacaggc gcgacctaga ttccgacaac gacgggctca cggacctcta cgagtgcggc
     2521 ggtacggacg acgccgacaa ggacggagag gtcgattccg atacggacgc gaacctgaac
     2581 ggccttgcag atgaatacga tctcgcccag ggcgggaata tgctcaagtg cgccgacacg
     2641 gacggggacg gcatacgcga cttcctcgac attaattccg acaaccaggg cggaacggat
     2701 tatgaagaga acggcggcac ggacacggac ggcgacggac tgcccgacac gaccgaggac
     2761 gcgaacgccg acggcctcct cgacgtattt gactcccaaa gggggggcgc tccgctcgag
     2821 ataagggact cggacggcga cggcatacct gaccagctcg accccgagaa aagcggagac
     2881 aacggaggca gctgcgctct cgctccggca ggaagcgaca catcggacgc tgcgcttctc
     2941 ccgctccttc ttttaccggc tctaatcctt ttcaggaggt ccttacgtat atatagtaag
     3001 gactgacgaa cagtcccggc catacaggtg tcaggggttc tctctccgta aaaacccctg
     3061 acactcctct actttaagca ggcaagctcc accttgtcat ttcgagcaac agttctgagc
     3121 aagcgaggaa tcggtcgctc tatactaaca gacaatgtgc cactttctgg gctccgttat
     3181 tttctactgg caaatcggct aaagaaaatc gattgtttac gcgtcaaaaa catttttggg
     3241 tacgaatgca agctagcgcg cattcggacg ccttacctta tccaaacggc gcattttaca
     3301 tcgcacatca tcagactaca tcgctccatt gggtggaata aaaatgtttt agcgtaaaca
     3361 tgaggttttt agtaaattct gttctgtctt ttcataaaac acactacgtg tgtgattttt
     3421 gcttcttttc atcaaggaaa agaagaaatc aatttcgcct cccgattgca ttaaaatccc
     3481 aaaaatacta taatccccct aaaaaaatcg cccgctccga tacatgcaag cgcaagaaac
     3541 caggctcctt ccgcacaaaa agaccgtcat cgcgagctcc ctcgccataa tcctcgaatg
     3601 gtacgacttc tcgatcttcg gcttctacgc ggtcgtgctc tccgagatct tcttcccgtt
     3661 cacggacgag actaccgcca tactctcgac gttcatgata ttcgcgctct cgttcctctt
     3721 ccggcccctc gggtcggtcg tcatttcgag cctcggcgac aggttcggga ggaagaacgc
     3781 gttcctcttc accgtcattc tcatgacgtt cccgaccgtg ctcatagggc tcctgcccgg
     3841 ctatgggaag atggggatcg ctgcgacggt gctgctcatc atactgagaa tagcccaggc
     3901 gctttccgtg ggcggcgagc gctcggccac gctctctctt ctcgcggaga tcgcgccccc
     3961 gcgcctcaga ggactctacg gcagcatgtc cctcttcagc actactgccg gaatactcct
     4021 cgcctcattc gtatgcgggc tcgtttcgac cgcgcttgat cacgacgagc tcgccggctg
     4081 gggctggagg atacctttcc tcctcggggc cgtctcgggc ctggccgcgt actcgctcag
     4141 gaaaactgtg agtgagtcgg ccatgttcct cgacctcaaa aattcaggga acacgtccaa
     4201 aagccccgtg agagagtcgc taaccgggca ctggaagccg gtgctcatta tcttcgcggc
     4261 gacgataatg ttcgccgtga gcttctacct catattcgtc tatatcccga ctttcgctac
     4321 ccggtacgga ggcatgactc tcgcgcagat actgaacatc aacacgatga acctcggcct
     4381 ctgcacgatg ctcgtccccc tgagcggcta cttctcggac agagtgggaa ggaagcccgc
     4441 aatcatcacg ggctgcgcgg gtgaggtcgt catggggttc ttgctgttca aggtgttcgc
     4501 ggggaacgac atcacgctca agataatcgt ccagttcgga gcagggctca tgaacgtgat
     4561 tttcgccgga gccttcgccg cctacatggt cgagagcttc ccgacgaggg tgaggatgag
     4621 cggcatatcg atggggaacg tgatcgggtt ctccattttc gggggctcgg cccctctaat
     4681 cgcgtcatac ctcatcgaca ggacgggcga cgtcaccgcg ccgggcatat acctggccgc
     4741 ggcatcggcc gtctccctca tcgctgtatt attcgcgaag gagacgtaca aaagcgagct
     4801 aaggtgatgg cgagaaaagg aagacatccc gctcatcctt cgacaagctc aggacgaacg
     4861 gtttttttct atcagtcatc ctgaatttat ttcaggatct catctttaaa tccgtaggag
     4921 cggcttccag ccgcaaatat gagtccgtca tttcgagtcc ttcgacttcg ctcaggataa
     4981 actccgtgcg gcaatctcat tcttcatcct tccccgacat tacagaattt gcggcacaca
     5041 atgtgtgctc aagcacaatc attccgaatc tcttctgcct aacccgcaaa tgatcccatc
     5101 ccgttcgccc tgagtttgtc gaagggttga acgggtatca cttctgttga aatgtgggtt
     5161 gaattgaacg attttagcct taatgcgaga tttttagtaa attctgctct gtcttttcat
     5221 aaaacacact acgtgtgtga tttttgcttc ttttcatcta ggaaaagaag aaatatttat
     5281 tgtttttctt tagtttttgc acacacacaa gatttgaacc gatggcgcga gtctactccc
     5341 caatcctctt cccgcaatgc gggcaaatct tcacgaccga ctcttttttc cgcatctcct
     5401 ctacgaaccc cgaagcgata atacccgccg gtatcgcgaa gagcgctatg ccgagcatgg
     5461 acacgcaggc cgctacgagc ttccccagcg gcgagagcgg gagcacgtcc ccgtacccga
     5521 ccgtgctcag cgtcacgacg gcccaccaca tggcggccgg gatgctcgag aacgcctcgg
     5581 gctgcttgtc gtgctcgatg tagtacatga ggctcgacga aagaataata agcatgagca
     5641 cgaggatggc cgaaatgacg atctccctcc tcttcgctcg gagcacgttc ccgaagagct
     5701 tgaactcgtc cgcatagcgg gtcatcttga ggagcgtcag cagcctgagc acgcggagga
     5761 aacggagatc gacagtcgtc acgaacggca ggtagaaggg gagtatcgcg atgaggtcca
     5821 cgagagcgaa cgccgagaat atgtatttta ttcttcccgt caccgggtgt ctgtacctct
     5881 cgtcgaccgt gcaggtccag agcctcagga gatactcaac ggtgaatatc acgatagaga
     5941 cgtcgttgaa tacattgaag aacgcgccga acctggcctc gatgctgtcc acggtctcga
     6001 gtatgaccgc gatcacgttg agcacgatga ggcccatgat ggagaggacg aatatctgct
     6061 cggcgacggg gctcgtatcg tcgggctcca tcacgtcgtg gattctgtac ttgagctttt
     6121 caagcattgg cgtccaataa ttctaaggac ttacgccggc cattgcaaga tataaggcaa
     6181 atggcgggtc gtacgcccca cttgcctctt ctgcccgctc atatatcctt actatcccgt
     6241 atatgaaaac acgtctcgcg cacgtaagca tcacggtgaa ggacctggac aggctcgcgg
     6301 agttctaccg gaaggcgctc gggttcacgg aggtccggcc ggaaaagggc ttctcgggcg
     6361 cctggttaga gaagggcacg ggcgtccccg gagcctcgat aaggagggtt cacctgcgcc
     6421 tgcccggctc aagccccgac gtcgcgctcc tagagctgat cgagtacgca ggcaaggcag
     6481 aggacaaatc gcctcccgca gcggacagca ccgggctcag gcacatagcg atagagaccg
     6541 ggagcgcgtc agagctcgcg aggctccgcg atctcgtcat agagaacggc ggctccggcc
     6601 tgggagatat atcggaaaat cgcatagacg gcctcggcac ggtcacgttc atctacatga
     6661 ccgacccgga ggggaatatc gtcgaactgc tcaactggcg ggaatcactc taagcctgct
     6721 aaagaatgtt actactatga cagtaaaaag gcctgattat ttggagcggg ccttcttgta
     6781 agctacaggg gtgcggagct tcccgccgtt catctcgtac ttatccgtgg tcatcatcct
     6841 gagcctgtac ttggagtcgt agccgttcgt gcagtcgtta caggaataac ttctcctcac
     6901 cttcctgtag aagtggacgg ccttcctgca tacggggcac acgcccacgt acttccccct
     6961 cggcatgatg agcccgtcca cgggggcgca cctctcgggc ctgcacccta ccctcctcgc
     7021 gacctctctc catccgttgt cgtggttcgt cttccccgtc tcgatgtagt gtatcgcatg
     7081 cgcgatctcg tggagcagag tgtccttcat cacagggtaa gggttggcgt tcatgaacgc
     7141 gagagagagc gagattatct tcctccgggg cttgcagtaa ccgaggtgtc tcctttggtt
     7201 cgagaacacg agctt
//
LOCUS       DASSJH010000106         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_241839, whole genome shotgun sequence.
ACCESSION   DASSJH010000106 DASSJH010000000
VERSION     DASSJH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241839"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFJ67_02275"
     CDS             <1..349
                     /locus_tag="VFJ67_02275"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796273.1"
                     /note="catalyzes the formation of biotin from dethiobiotin
                     and sulfur 2 S-adenosyl-L-methionine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET7289238.1"
                     /translation="EQRIELAFELRELGPSWVPINFLNPRPGTPFEKFQALSPLEAVK
                     TIAIFRLILPDKILMTAGGREVTLRDLQSMGLVAGANAMILGNYLTTPGRTPEEDLRM
                     LDDLEMPVRKDVN"
     gene            415..1773
                     /gene="bioA"
                     /locus_tag="VFJ67_02280"
     CDS             415..1773
                     /gene="bioA"
                     /locus_tag="VFJ67_02280"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET7289239.1"
                     /translation="MSDKKKNSGAFDREFVWHPFTQMKEYEGKEPLVIERGEGAYLID
                     SDGRRYIDGVSSLWVNIHGHRVPEIDNAIKEQVDSLAHSTLLGVTNPPAALLAKELID
                     ICPPGLRKVFYSGDGASAVEVALKMAFQYWQHKGEPGKTKFVSLGNAYHGDTLGAVSV
                     GGIDLFHSAFEPLLFETYQAPSYYCYRCPLGKSYPSCGLACADALGKILEDHRDQIAG
                     VILEPYVQAAGGMIVSPPGYLKRVREYCDTYGVLLILDEVATGFGRTGRMFACEHDGV
                     TPDILVIGKGMTGGYLPLSATITTQDVYDAFLGEYEEFKTFFHGHSYAGNPLACAASL
                     GNLAAFRKYDTLRNLKEKIEYLEEELREFSGLRHVGNVRSKGLMAGIELVLDKDTKEP
                     YPARMKMGWRVADEALKEEVLIRPLGNVVVLMPPLGIPMEDLKKLVRATYNSIKRATE
                     AA"
     gene            1825..3003
                     /gene="bioF"
                     /locus_tag="VFJ67_02285"
     CDS             1825..3003
                     /gene="bioF"
                     /locus_tag="VFJ67_02285"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HET7289240.1"
                     /translation="MNKTLDWIENDLAHIRDKKLFRALTELGTGQSPEITVGGKRYIL
                     LASNSYLGLSIDPAVVDAARRALEKYGTGSGGSRLVSGSTDLHRELEERIASFKSTES
                     AIIFSSGYLANIGTISSLVGEGDTVYSDELNHASIIDGCRLSRADIRIYRHLDYRHLE
                     SLLKSGSGKKGKKLIVTDTVFSMDGDLASLPALVGLSENYGCMLMVDEAHATGVLGVR
                     GSGATEHFGVEERVPIVMGTLSKAIGSLGGYIAGSRELTDFIRNRVRSYIFDTSLPAA
                     SLAASVAAIDIIEKEPSRREHLWRLIKMFKSGIVDMGLKVLPSDSAIVPVLVGEAEPA
                     LEFAAALMECGIYTPAVRPPSVPEGKCRIRATLMAAHTEEHVGLALSAFKTASVKCGL
                     "
     gene            complement(3093..>3851)
                     /locus_tag="VFJ67_02290"
     CDS             complement(3093..>3851)
                     /locus_tag="VFJ67_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7289241.1"
                     /translation="ADDAAGLLRTLGIGPVHVLGISLGGFIAQELALRHPDLVRSLVL
                     VSTNSGRGSSVMRNSPHLNGLLKLWGILPGSFEMTGKASAPLGIEYGLTKEEKIRYGL
                     SLAFTPEYYKNHPEEIERIVRWRLESPQPGYAWARQFTAGTHFDSSGRAEGINAPALV
                     INGSDDRIVSPESARELAERIPGARYVELEGTGHLLFIERADEFNETVIDFLDEVRID
                     DDTAAGSRDDDGWWHKIVSLISGGNGRKGSSLKN"
BASE COUNT          819 a         1110 c         1136 g          786 t
ORIGIN      
        1 ggagcagcga atagaactcg ctttcgagct gagagagctc ggcccgagct gggtgccgat
       61 caacttcctt aacccgaggc ccgggactcc cttcgagaag tttcaggcgc tgtcccccct
      121 cgaagccgta aagacgatcg ctattttcag gctcatactg cccgataaga ttctcatgac
      181 agccgggggg agggaggtca ctctcaggga tctccagtcg atggggctcg tcgcaggcgc
      241 aaacgcgatg atcctcggca attatctcac cactccgggc aggaccccgg aggaagacct
      301 caggatgctc gatgacctcg agatgcccgt cagaaaggac gtcaactgac agtcccctgg
      361 tcaggcccct tccggttccg gccattttcc ccgtaatcct cggcagacag aaaaatgtcg
      421 gataagaaaa aaaattcagg agcttttgac agggaattcg tatggcaccc ctttacccag
      481 atgaaggagt acgaagggaa agaacccctc gtcatcgagc gcggggaagg cgcttacctc
      541 atcgactcgg acggcaggag gtatatcgac ggtgtttcgt cgctctgggt gaatattcac
      601 ggccaccggg ttcccgagat agataacgcg atcaaggagc aggtggattc gcttgctcac
      661 agcactctgc taggcgtcac gaacccgccc gcggctcttc tcgcgaaaga gctcatagat
      721 atatgcccgc cggggttgag gaaggtcttt tattcgggcg acggcgcgag cgctgtcgag
      781 gtcgcgctca aaatggcttt ccagtactgg cagcacaaag gcgagcccgg caagaccaaa
      841 ttcgtctcac tcggtaacgc ctatcacggc gatactctcg gggcggtctc tgtcggaggc
      901 atcgacctct tccactcggc gttcgagccc ctcctgttcg agacatatca ggccccgtct
      961 tattactgtt acagatgccc gctcggaaaa agctatccct cctgcgggct cgcttgcgcg
     1021 gacgccctcg ggaagatact agaggaccat cgcgatcaga tcgcgggagt catactggag
     1081 ccgtacgtgc aggccgcggg cggaatgata gtttcccctc cgggttattt gaagcgcgtg
     1141 cgcgagtact gcgacaccta cggcgtgctg ctcatactgg acgaggtcgc tacggggttc
     1201 ggcaggacag ggcggatgtt cgcctgcgag cacgacggcg ttacgcccga tatactcgtc
     1261 ataggaaagg gcatgaccgg gggctacctc ccgctctcgg ccacgataac gacacaggac
     1321 gtctacgacg cgttcctcgg cgaatacgag gagttcaaga ccttcttcca cggccacagc
     1381 tacgccggga accctctcgc ctgcgcagct tccctgggca acctcgcggc gttcagaaag
     1441 tacgacacgc tccggaacct taaagaaaag atcgaatacc ttgaagaaga gctcagggaa
     1501 ttcagcggcc tcaggcacgt cgggaacgtg aggagcaagg gattgatggc cggtatagag
     1561 ctcgttctcg ataaagacac gaaggagccc tatcccgccc gcatgaagat gggctggagg
     1621 gtcgcggacg aggcgcttaa agaagaggtg ctgatacgcc cgctcggaaa cgtcgtagtc
     1681 ctcatgcccc ctctggggat tcctatggag gatttgaaga agctcgtccg cgcgacgtac
     1741 aattctatca aaagggcgac cgaagccgcg tgacggtcat ttcaatagac tgcattcgtg
     1801 tgacggtatg ccggtttccg aattatgaat aagacactcg actggataga aaatgatctg
     1861 gctcatatca gggacaagaa gctgttccgt gcgctcaccg agctcgggac ggggcagtca
     1921 cccgaaataa cggtcggcgg gaagcgctac atactcctcg cctcgaacag ctatctcggc
     1981 ctctctatcg accctgctgt ggtggatgcc gcgcggcggg cgctcgagaa atacgggacc
     2041 ggctccggcg gctctcgtct cgtgagcggg agcaccgacc tccacaggga gctcgaggag
     2101 cgcatagcgt ccttcaagag caccgagtcg gcgataatat tcagctccgg ttacctcgcc
     2161 aacataggca ctatttcttc gcttgtcggc gagggagaca ccgtctacag cgacgagctc
     2221 aaccacgcct ccatcatcga cgggtgcagg ctctcccgcg cggacatacg aatatacagg
     2281 cacctcgatt acaggcacct ggagtcgctc ctcaagtccg gcagcgggaa aaaagggaag
     2341 aagctcatag tcacggatac cgttttcagc atggacggcg acctcgcctc cctccctgct
     2401 cttgtcgggc tctcggaaaa ttacggctgc atgctcatgg tggacgaggc tcatgcaacg
     2461 ggggtgttag gcgtaagggg cagcggagca accgagcact tcggcgtcga agagcgtgtc
     2521 cccatagtca tgggaacgct ctctaaggcg ataggctcac tcggcggcta catcgcgggc
     2581 agccgggagt tgaccgattt catcaggaac cgcgtcagga gctatatctt cgacacttcc
     2641 ctgcctgcgg catccctcgc ggcgtccgtt gccgcgatag atataataga gaaagagcct
     2701 tctagaagag agcatctctg gaggcttata aagatgttca agagcggaat agtggatatg
     2761 ggcctaaagg ttctgccttc cgattccgcg atcgtgcctg ttctcgttgg cgaggcggag
     2821 cccgccctcg agttcgccgc tgctttaatg gagtgcggca tatatacgcc cgcggtgagg
     2881 ccgccctcgg tgcctgaagg gaagtgccgt ataagggcga cgcttatggc agcgcacacg
     2941 gaagagcacg tcgggcttgc tctctcggcg tttaagaccg cgtccgtgaa gtgcgggttg
     3001 tgaagctgag agtatcgaac acgttgggaa atatgattcg cggagcttgt ccggaattga
     3061 ttaccggggg tgtgagcggg aatagccggg tcttaattct tgagagagct tcctttcctg
     3121 ccgtttccgc ctgagattag agagactatt ttgtgccacc acccgtcgtc atcccggctt
     3181 cctgcggccg tatcatcatc gatgcgcacc tcgtcgagaa aatcgatgac tgtctcgttg
     3241 aactcgtccg ccctctcgat gaataacaga tgtcccgttc cttccagctc tacgtacctc
     3301 gcaccgggta ttctctccgc gagctcgcgc gcgctctcgg gcgagactat cctgtcgtca
     3361 gagccgttga taacgagcgc gggcgcattg atcccttccg cccgtcccga ggaatcgaag
     3421 tgcgttcccg ccgtaaactg acgggcccag gcgtaaccgg gctgcgggct ctccaatctc
     3481 cacctgacaa tacgctctat ttcctcagga tgatttttgt agtattcggg agtgaaagcg
     3541 agagagagtc cgtaccggat cttttcttct ttcgtgaggc cgtattcgat cccgagaggc
     3601 gcgctcgcct ttccggtcat ttcgaatgac cccggcagta tgccccagag cttcaggagg
     3661 ccgtttaagt gaggggaatt cctcatcacg ctgctcccgc gtcccgagtt cgtggatacg
     3721 agaacgaggc tcctgacgag gtccggatgc ctgagagcca gttcctgggc gataaacccg
     3781 ccgagggata ttccgagtac gtgcacagga cctatcccga gggtcctcag gaggcctgcg
     3841 gcgtcgtcgg c
//
LOCUS       DASSJH010000107         5554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_674822, whole genome shotgun sequence.
ACCESSION   DASSJH010000107 DASSJH010000000
VERSION     DASSJH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674822"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /gene="purD"
                     /locus_tag="VFJ67_02295"
     CDS             <1..1245
                     /gene="purD"
                     /locus_tag="VFJ67_02295"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET7289242.1"
                     /translation="LYCAPGNPGISRHAECVDIKISDIEGLLRFARAEEIDFTVVGPE
                     FPLSLGIADRFTEEGLGVFGPSRAASEIETSKVFSKNLMRKYGIPTAFFSAFDDFEQA
                     VEWVKEVKPPLVIKADGLASGKGVFICRTESEAVEVLDDIMRRKIFGDSGDRVVIEEF
                     LSGEEASFFAVTDGANVLPLESCQDHKALLDGDQGPNTGGMGAYSPAPVITPEMSQEI
                     MSSIIIPTVKALRDEGREYKGVLYAGLMIEDGKARVLEYNCRFGDPETQPLMMRMKSD
                     IVPVLSAVAGRGLSGERVEWRDEASVCVVMASRGYPGDYKKGAHITGLDALEDMENVM
                     VFHAGTAIGNGGIVTDGGRVLGVTALGRTIPDAINLAYQAVGKVNCDSLCFRSDIGKK
                     ALRHVHLTGSSGGPSREEANNG"
     gene            1238..1552
                     /locus_tag="VFJ67_02300"
     CDS             1238..1552
                     /locus_tag="VFJ67_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289243.1"
                     /translation="MAEIWYLPVDSESVEGREAQYERPFRDTIEILELTPEKWQCGPG
                     EFPELKTGDPFVDESGFVYVLMRVGEDEVAKYDDKRWKPGWYKSSLTIVGSEKNLRKK
                     PK"
     gene            1631..2002
                     /gene="rpsL"
                     /locus_tag="VFJ67_02305"
     CDS             1631..2002
                     /gene="rpsL"
                     /locus_tag="VFJ67_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET7289244.1"
                     /translation="MPTISQLVKDGRIRAKKKSKAPALQNCPQRRGVCVRVYTTTPKK
                     PNSALRKVARIRLTNGIEVTGYIPGEGHTLQEHSVILVRGGRVKDLPGVRYHIVRGTL
                     DSTGVANRRKSRSKYGAKKPK"
     gene            2021..2491
                     /gene="rpsG"
                     /locus_tag="VFJ67_02310"
     CDS             2021..2491
                     /gene="rpsG"
                     /locus_tag="VFJ67_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7289245.1"
                     /translation="MPRKGSVSKRKVPVDPKFGDLTVAKFINSLMQDGKKSKAEMIFY
                     GSLKLIGEKTGKDPIEVFNAAMDNVKPGIEVRPRRVGGATYQVPMEVSSFRKQSLAVR
                     WILSSARKRDGKSMIDKLTGELLDASEGRGGAIKKREDTHKMADANRAFAHYRW"
     gene            2511..4592
                     /gene="fusA"
                     /locus_tag="VFJ67_02315"
     CDS             2511..4592
                     /gene="fusA"
                     /locus_tag="VFJ67_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7289246.1"
                     /translation="MEKAIPINKVRNIGIVAHIDAGKTTTTERILYYTGLTYKIGEVH
                     EGTATMDWMEQERERGITITSATTTCFWNDHRINIIDTPGHVDFTIEVERSLKVLDGA
                     VVVFDSVGGVEPQSETVWRQADRYRVPRMCFVNKMDKIGADFAKVVSQIKDRLSANPV
                     PLHSPYFEGDNFKGIIDLIRMKMALWDEDSLGLKYDWYDIPAEMKDEAVAGREKLIES
                     LADFDETIMEKYLNGESVSQEEIEASIRKSTVDLNIVPVILGSAFKNKGVQLLLDSIV
                     CYMPSPLDVPPVEGINPDTEQGEVRRPNDDEPFSALAFKIMTDPFVGQLTYLRVYSGK
                     LVSGSTMYNSTKTKREKIGRLVRMHSNKREDIKEIGAGGIAAAVGLKVTTTGDTLCDE
                     DKPIILESLYFAEPVISIAIEPKTKSDQEKLGISLNKISLEDPTFKVKYDDETGQTII
                     SGMGELHLEIIVDRLKREFNVDANVGKPEVAYRETITKSSDVEAKFIRQTGGRGQYGH
                     VKIKMERGEEGSGVTFVDEIKGGTIPKEYIPAVEKGVKEASETGVVAGYPVIDVVVKL
                     YDGSYHEVDSSEIAFKIAASMAFKDAVMRAKPIILEPLMKVEVVVPEDFMGSVMGNLS
                     SRRGKITNTELRGNMQAIKAEVPLSEMFGYATELRSMTEGRGSFTMEFSHYSALPELL
                     SENLKTNVRAV"
     gene            4637..>5554
                     /gene="tuf"
                     /locus_tag="VFJ67_02320"
     CDS             4637..>5554
                     /gene="tuf"
                     /locus_tag="VFJ67_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7289247.1"
                     /translation="MAKEKFARGKPHLNIGTIGHVDHGKTTLTAAITKVLSKAGSAKY
                     VEFAEIDKAPEERERGITINIAHVEYETPKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAPDGPMPQTREHILLARQVGVPYIVVALNKVDMMDDEELLDLVELEVRDLLTQ
                     YGFPGDDVPVVRVSALKALEEDPTAMNQIMDLMKAVDEYIPEPVREMDKPFLMAIEDV
                     FTISGRGTVVTGRVERGRVVAGDEVEIVGFGDTRKTVATSLEMFRKILDDAQAGDNVG
                     VLLRGTGKDEVERGQVLAKPGSITPHKKFK"
BASE COUNT         1459 a         1301 c         1761 g         1033 t
ORIGIN      
        1 ctatattgcg cccccgggaa tcccggaatc tcgagacacg ccgagtgtgt cgatataaag
       61 atttcggata tcgaggggct gctccggttt gcacgggccg aggagataga cttcaccgtc
      121 gtcggtcccg aattccccct ctccctcggc atcgcggaca ggttcacgga agaggggctc
      181 ggcgtattcg gcccgtcgag agccgcctcc gagatagaga cgagcaaggt cttctcaaag
      241 aaccttatga ggaagtacgg cataccaacc gctttcttct cggcgtttga tgatttcgag
      301 caggccgtcg agtgggtgaa ggaggtgaag cctcctctcg ttattaaagc cgacgggctc
      361 gcttcaggaa aaggcgtgtt catatgccgg accgagtcgg aggcggtcga ggttctcgac
      421 gatatcatga gacgaaagat attcggcgat tcgggcgaca gggtggtcat tgaagaattt
      481 ctttcgggcg aggaagcgtc cttcttcgcc gttacggacg gcgccaacgt gctcccgctc
      541 gaatcatgcc aggaccataa ggcgctgctc gacggagacc aggggcctaa tacgggcggc
      601 atgggcgctt actcgcccgc gccagtgata acgcccgaga tgtctcagga gataatgagc
      661 tcgatcataa taccgaccgt caaagcgctc agggacgaag gcagggagta taaaggcgtg
      721 ctctacgccg gcctcatgat agaggacggc aaggcgagag tcctcgaata taactgtaga
      781 tttggggacc ctgagactca acccctcatg atgaggatga agtccgacat cgtgcccgtt
      841 ctctcggctg tcgccggcag gggactctcg ggagagaggg tcgagtggag ggacgaggcg
      901 tccgtgtgcg tggtgatggc ctccaggggt tatccgggcg actataaaaa aggggcgcat
      961 ataaccgggc tcgacgccct cgaagacatg gaaaacgtca tggtcttcca cgcgggaacg
     1021 gctataggaa acggcggcat agtgaccgac gggggcaggg tactgggcgt cacggcgctc
     1081 ggccgcacga tacccgatgc aataaacctt gcataccagg cggtggggaa ggtaaactgt
     1141 gattccctct gcttcaggtc cgatatagga aagaaggcgc tccggcacgt tcacctgacc
     1201 ggctcttcgg gcgggcccag cagagaggag gcgaacaatg gctgaaatct ggtatctacc
     1261 cgtcgattcg gaatcggttg agggcaggga ggcgcagtac gaacgccctt tccgggacac
     1321 aatcgagatt ctggagctta cgcccgagaa atggcagtgc ggccccggcg agtttccgga
     1381 gcttaagacc ggggatccgt tcgtggatga aagcgggttc gtatatgttc tgatgagggt
     1441 gggtgaggac gaggtggcga agtacgatga caagaggtgg aagcccggct ggtacaagtc
     1501 ctccctgact atagtggggt cggagaaaaa tctcaggaaa aaacctaaat aattagtctt
     1561 tttgtcttga cagagatttc cttttcggga tatattaatt ggattacctt ttttacggag
     1621 ataagcattc gtgcctacaa ttagtcagct ggtcaaagac ggaagaataa gggctaaaaa
     1681 gaagagtaaa gcccctgcgt tgcagaattg tccacagagg agaggggtct gcgtcagggt
     1741 ttatacaacc accccgaaaa agccgaattc ggcgctcagg aaagtggcac gaataaggct
     1801 cacgaacggt attgaggtaa cgggttatat accgggggaa ggacacacgc tacaggagca
     1861 ctccgtaata ctggtaaggg gcggtagggt gaaggacctg ccgggggtga ggtaccacat
     1921 cgttaggggt acgctcgact cgaccggcgt ggcgaacaga aggaagagcc gttcgaaata
     1981 cggggccaag aaacccaaat aatcaaaggg aacaacagat atgcctagga agggatcagt
     2041 ttctaaaaga aaagttcccg tcgatcccaa gttcggtgat ttgacggtcg cgaagttcat
     2101 caactcgctc atgcaggacg gcaaaaagag caaggcggaa atgatattct acggttcgct
     2161 gaagctgatc ggggaaaaga ccggcaagga cccgatcgag gtattcaacg cggccatgga
     2221 caacgtaaag ccgggtatcg aggtcaggcc gcgcagggtc ggcggcgcca cgtatcaggt
     2281 gccgatggaa gtgagctctt tcaggaagca gtccctcgcc gtaaggtgga ttctttcctc
     2341 ggcgagaaag agggacggaa agtcgatgat agacaagctc accggtgagc tgctcgacgc
     2401 ctcggaagga cgcggcgggg ccattaagaa gagagaagac acgcacaaga tggcggatgc
     2461 gaacagggct tttgcccatt acaggtggta ataaaccgcg gaggagtatt gtggaaaagg
     2521 ctataccaat taataaagta aggaatatcg gtatcgtggc gcatatagat gcgggcaaga
     2581 cgactacgac cgagcgcata ctttattaca ccggacttac ttacaagata ggcgaggtcc
     2641 acgaaggcac ggccacgatg gattggatgg agcaggaaag ggagaggggc atcacaatca
     2701 cctcggccac gacaacctgt ttctggaacg accacaggat aaatatcatt gacaccccgg
     2761 ggcacgttga cttcacgata gaggtcgaaa ggtctctcaa ggtcctggac ggagccgtag
     2821 tagtgttcga ctcggtgggc ggggtagagc cccagtcgga gaccgtatgg cgccaggcag
     2881 acagatacag ggtccccagg atgtgtttcg ttaataaaat ggacaagata ggggccgatt
     2941 tcgcgaaggt cgtctctcag ataaaagaca gactcagcgc caaccccgta cccctccact
     3001 cgccttactt cgaaggcgac aatttcaaag gaataataga tctgataaga atgaaaatgg
     3061 ccctctggga cgaggattcc ctggggctta aatacgattg gtatgacatc cccgccgaaa
     3121 tgaaggacga agccgtagcc gggagggaaa agcttataga gtcccttgcc gacttcgatg
     3181 aaacgataat ggagaagtac ctgaacgggg aatccgtttc tcaggaagag atagaggcct
     3241 cgataaggaa atccaccgtc gacctcaaca tcgtccccgt gatactcggc tccgcattta
     3301 aaaacaaagg cgtgcagctt ctcctagatt cgatagtgtg ctacatgccc tcaccgctcg
     3361 atgtgccgcc tgtcgagggc atcaatccgg acacggagca gggcgaggtc aggcgtccaa
     3421 acgacgatga gcctttttcg gcgctcgcgt tcaagatcat gacagacccc ttcgtcggac
     3481 agctcacgta ccttagggtt tattccggga agctcgtttc gggcagcacc atgtataact
     3541 ccaccaagac gaagagggag aagatcggca ggctcgtcag gatgcactcc aacaagaggg
     3601 aggatatcaa ggagattggc gcgggcggta tagcggcggc ggtgggcctc aaagtgacaa
     3661 cgacgggcga cacgctctgc gacgaggaca agccgataat tctagaaagc ctatatttcg
     3721 ccgagccggt gatatcgatc gcaattgagc ccaagactaa atcggaccag gaaaaactag
     3781 gcatttccct caataaaata tccctcgaag accccacatt taaagttaaa tatgatgacg
     3841 aaaccggcca gacaatcatc tccggcatgg gcgaattgca cctggaaatt atagttgacc
     3901 gcctcaagcg cgagttcaac gtggacgcca acgtcggcaa gcccgaggtc gcgtacaggg
     3961 aaacaattac caagagctcc gacgtcgagg ccaagtttat aaggcagacg ggcggcagag
     4021 gccagtacgg acacgtaaaa atcaagatgg agcgcggaga agaggggtcg ggtgttacgt
     4081 tcgttgacga gatcaagggc ggcaccatcc cgaaggaata tataccggcc gtggaaaagg
     4141 gcgttaaaga agcttccgag acgggtgtcg tcgccggcta tccggtcatc gacgtcgtcg
     4201 tgaagctcta cgacgggtcc taccacgagg tagacagctc cgagatcgcg ttcaagatag
     4261 cagcgtcgat ggcgttcaag gacgccgtaa tgagggcgaa acccataatc ctcgagcccc
     4321 tgatgaaggt cgaggtcgta gtgcccgagg acttcatggg ttccgtgatg ggtaatctga
     4381 gctcgaggag gggtaagatc accaacaccg agctccgcgg aaacatgcag gctataaagg
     4441 cggaagtccc gctgtccgag atgttcggtt atgcgaccga gctcaggtcg atgaccgagg
     4501 gcaggggttc attcacgatg gaattttctc attattctgc gttaccggaa ttactttcgg
     4561 aaaatcttaa aactaacgta agggccgttt aatagtacag gccgcgaact tcacgtgaag
     4621 gaggtataag caggcgatgg cgaaggagaa atttgcgcgg ggaaagccgc acttgaacat
     4681 agggacgata gggcacgtag accacgggaa gacgacattg acggcagcga taacgaaggt
     4741 attgagcaag gccgggagtg cgaagtacgt ggagtttgcg gagatagaca aggcgccaga
     4801 ggagagggag cgagggataa cgatcaacat agcgcacgtg gagtacgaga cgccgaagag
     4861 gcactacgcg cacgtagact gtcccgggca cgcggactac gtaaagaaca tgataacggg
     4921 ggcggcgcag atggatggcg ctatattggt agtgagcgct ccggacggtc ctatgcccca
     4981 gactagggag cacatactgc ttgcgaggca ggtaggagtg ccttacatag tggtagcgct
     5041 taacaaagtg gacatgatgg acgacgagga attgcttgac ctggtggagc ttgaggtaag
     5101 ggacctgcta acgcagtacg ggttccccgg ggacgatgtg cctgtggtga gggtgagcgc
     5161 gttaaaggct cttgaggaag accctacggc gatgaaccag ataatggatt tgatgaaagc
     5221 ggttgacgag tacataccgg agcccgtgag ggagatggac aagccgttcc tgatggcgat
     5281 agaggacgtg ttcacgatct ctggaagggg gactgtggta acgggcagag tggagagggg
     5341 aagggtagtt gcgggggacg aggtagagat agtggggttc ggggacacgc ggaagacggt
     5401 tgcgacgagt ctcgagatgt tcaggaagat actggacgac gcgcaggccg gggacaacgt
     5461 gggagtgtta ctcaggggaa cgggtaagga cgaggtcgag agggggcagg tgctcgcaaa
     5521 gcccgggagc ataacgcctc acaagaagtt caag
//
LOCUS       DASSJH010000108         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_133284, whole genome shotgun sequence.
ACCESSION   DASSJH010000108 DASSJH010000000
VERSION     DASSJH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133284"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="VFJ67_02325"
     CDS             complement(<1..2226)
                     /locus_tag="VFJ67_02325"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET7289248.1"
                     /translation="MNLKAYLATFRVKIALKITLGGVIAALICNFFHLPSGFLAPVIL
                     YMIMSGFHGQTFEVGVQSLIGSMLTAVYSLLIVYYFLDSKPIYLILAAVWIFACITFI
                     GKYAIASILSAILAAMTMFVAVFGTVSQTTASVENYIVQLFVAVTVSWLVDEIIWPHR
                     SGGALYLTLSSVYKDYAGRFASYIRSGGKGMQAGRFESASVDVFNNLVNLVRRTERET
                     RDGSFHAEPVLMLVAYAKSIYIKLDVLGEIMSVEHRCFEDEAVSEGLNGLFAALSGGF
                     GEMSAAIGENRPARSQPGDLDTKITDLKKLYESMHSAEGKDQDYFEDLLALGAVLPLF
                     EETAGLLGKADGVWNLINSGGYDKLLKDRVTRSPGVERIKSAIIPHITKESAHQSIKS
                     VIVIMLLLFGELLLKLPGGYQASFYGVLFGSIPNTGQAHLRGRLAIIGVAAGLLYGIT
                     GLYIISLIPHFILLILIFSLGSFVSAYVASGSQRIAFSGLQAGLMLPYVFFSTTGPEI
                     SLTLATMRMFALLMASAIGLLVLHTIWPVSPYRELKKKISSALAISGTIFGKLLVLDE
                     AERDKIETLVDPLAAAMPTSSSLLFDAQYIICDEKLHAEDFVHIIESLEVIYAELETL
                     KKTMYAEIDNELLKNYLEHMAPDYKTLCGLFDRASRQFFSGDDPDTAALELSRRIQEH
                     RKEFRETGFWKQFSLEDVEKNVLITASVDTVLDALHKIMVSISNIREAEASPGTQLAA
                     SK"
     gene            2452..3285
                     /locus_tag="VFJ67_02330"
     CDS             2452..3285
                     /locus_tag="VFJ67_02330"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HET7289249.1"
                     /translation="MIIPHTMVGGALVLWYVLTGLSLIFLIYDLETNTPAQWVMKLAW
                     ILIVLYTGPLGLLIYMLSCRQPLEGTHDMFIAPHWKQSVGSLMHCVAGDATGIIIGAI
                     ITFHIGLPNGVDLIFEYIMAFIVGLLIFQALFMKSMAGGDYIEAVKKTFFAETVSMNF
                     VMVGMIPVMAILRMKIPGGDDPKGLMFWGISSLATIAGAVAAYPVNSWLVGSGHKHGM
                     MSAVSGRTNMERMPGMEGGMQHKTPHVTFMKKLGVFLISAGFLLAAVWVTSRIIALKL
                     H"
     gene            3350..4000
                     /locus_tag="VFJ67_02335"
     CDS             3350..4000
                     /locus_tag="VFJ67_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289250.1"
                     /translation="MQRGKVLEELKSEYSRKKDMIEAQLGEFRKIRESADDRKIFEEL
                     AFCILTSAVGPRVGQNSLEAIKDILLEGTADDLESSLKGIHKFPEKAYFIVHTRNYLK
                     KEYGFKLRELVDSFGDPETRRDFFATNKEIKGIGYTQASHFLRNIGFGGYAILDKNVV
                     KSLYDLGIIDSPKPPATGKKYKEIEERMKALAGELGIGLDELDMLLWSLKTGRIPV"
BASE COUNT          998 a         1108 c         1154 g          912 t
ORIGIN      
        1 ttttgacgcc gcgagctgcg taccgggaga agcctccgct tccctgatat tgctgatcga
       61 aaccattatc ttatgcaggg cgtcgagcac ggtatctacg ctcgccgtaa taagcacgtt
      121 tttctccacg tcttcgagag agaattgctt ccagaaaccg gtctcgcgaa actccttacg
      181 gtgttcctgt atccgacggg aaagctcaag cgcagccgtg tcaggatcgt ctccgctaaa
      241 gaactggcgc gaagccctgt cgaacaaacc gcagagcgtc ttgtagtcgg gagccatgtg
      301 ctccaggtag ttcttcagga gctcgttgtc tatctccgcg tacatggttt ttttcagggt
      361 ctcgagctcg gcgtatatga cttcgagcga ctcgattata tgaacgaagt cctcggcgtg
      421 gagcttctcg tcgcagatga tgtactgggc gtcgaaaagc agcgagctgc tcgtcggcat
      481 ggcagcggcc agcggatcga cgagggtttc tatcttgtcc ctttccgctt cgtcgagcac
      541 taacagctta ccgaatatag tcccgctgat cgcgagcgcg gacgatatct tctttttgag
      601 ctcgcgataa gggctgactg gccagatagt gtggagcacg agaagcccga tggcagacgc
      661 catgagcagg gcgaacatcc tcatcgtagc gagcgtgagg cttatctcag gcccggtggt
      721 cgagaaaaaa acatagggga gcatcaggcc cgcctggaga ccggagaatg ctattctctg
      781 gctgccgctc gcgacatacg cggagacgaa agatccgaga gaaaaaatca ggatgaggag
      841 aatgaaatga gggatgagcg agattatata cagtccggtt atgccgtaga ggagacccgc
      901 cgcgacgcct ataatcgcga gcctcccccg gaggtgcgcc tggcccgtat tcgggatcga
      961 gccgaagagc acgccgtaaa aagaggcctg ataaccgccg ggcagcttga gcaggagctc
     1021 gccgaaaaga agaagcatga tcactatcac cgactttatg gattggtggg ccgactcctt
     1081 cgtgatgtga ggaattattg cggactttat tctctcaacg ccgggagacc tcgtcaccct
     1141 gtccttgagg agcttatcgt atccgccgct atttatgagg ttccaaaccc cgtcggcctt
     1201 cccgagaagc ccagccgttt cctcgaacag tgggagcaca gccccgagcg cgagcaggtc
     1261 ctcgaaataa tcctggtctt tcccttccgc cgaatgcatg ctctcgtaga gtttcttcag
     1321 atctgtaatc tttgtatcga gatcgcccgg ctgagaccga gcgggacggt tctctccgat
     1381 tgcggcggac atctccccga acccgccgga cagcgcggcg aacaaaccgt ttagaccctc
     1441 ggaaacggcc tcgtcctcga agcaccggtg ctccacgctc atgatctctc cgagtacatc
     1501 gagcttgatg tagatgctct tggcgtaggc gacgagcatc agtaccggct cggcatggaa
     1561 gctcccgtcc ctcgtctcgc gctccgtacg ccttacgagg tttacgaggt tattgaagac
     1621 gtcgacggac gcggattcga accgtccggc ctgcatgcct tttcctcccg agcggatata
     1681 ggacgcgaac cgtccggcat agtccttata tacgctcgac agggtgagat aaagcgcacc
     1741 gccgctcctg tggggccata ttatctcatc gacgagccac gagaccgtca cggcgacgaa
     1801 tagctgtacg atgtagttct ctacggacgc ggttgtctgg gagaccgtgc cgaaaaccgc
     1861 gacgaacatg gtcatggccg ccaatatggc gctcagtatg gaggcgatcg catatttccc
     1921 tatgaacgtt atgcaggcaa atatccagac cgcggctagt ataaggtaaa tcggtttcga
     1981 atcgaggaaa tagtagacta tcaataacga ataaacagcg gtcagcatgc tgcctatcag
     2041 ggactggact ccgacctcga acgtctggcc gtggaacccc gacattatca tgtaaaggat
     2101 cacgggggcg aggaatcccg acgggaggtg gaagaaattg cagataagtg ccgcgatcac
     2161 gccgccgagc gtaatcttga gcgcgatctt gacgcggaat gtggcgagat atgccttgag
     2221 gttcatatat cacttacggg gacgccgcgc tgctcccgcc tgcaattata cagtctgtct
     2281 gtgccggtat cacggagatc ctcccgagcc cccgctccgg aaggccggct tttcagatta
     2341 tcacacgcag caccgaagta tgtcaatcgg ttgacaaggc attcttaatg atgagataat
     2401 ggcttcaata caagtatcga tcggccgata ttacaataat ccggggggaa aatgataata
     2461 cctcacacaa tggtcggggg cgcgctcgta ctctggtatg tattgaccgg attgtcgctc
     2521 attttcctca tctatgacct cgagacgaat acgcccgcac agtgggtgat gaagctcgcc
     2581 tggatattga tagtcctcta cactgggcct ctcgggcttc ttatatacat gctatcatgc
     2641 cgtcagcccc tcgagggaac gcacgacatg ttcatcgcgc cccactggaa gcagtccgtt
     2701 ggctctctca tgcactgtgt cgccggagac gcgacaggaa taattatagg cgcgataatc
     2761 acctttcaca tcgggcttcc gaacggtgtc gatctcatat ttgaatacat catggctttc
     2821 atcgtcggac tcctgatatt ccaggcgctc ttcatgaaat cgatggctgg cggagactac
     2881 atcgaggccg taaagaagac tttcttcgcg gagaccgtat cgatgaactt cgtcatggtc
     2941 ggcatgatcc ccgtgatggc catactgagg atgaagattc cgggagggga cgacccgaag
     3001 gggctgatgt tctggggcat ctcgtctctc gcgacgatag cgggagccgt ggccgcctac
     3061 cctgtaaatt cctggctcgt cgggagcggg cacaagcacg gcatgatgtc ggccgtatcg
     3121 ggcaggacta acatggagag aatgcccgga atggagggcg gtatgcagca taagactcct
     3181 cacgtcactt tcatgaaaaa actaggcgtg ttcctgatct cggccggctt ccttctggcc
     3241 gccgtttggg taacgtcgcg gataatcgca ctgaagctgc attaacccgt gctactctcc
     3301 gttcgattcg tccgagtcac ggtaaaaaat acatgataga cttactcagg tgcaaagagg
     3361 caaggtgctc gaagagctta agtccgaata ctcccgcaaa aaagacatga tagaagcgca
     3421 gctcggagag ttccgtaaga tacgggaaag tgccgacgac agaaagatat tcgaggagct
     3481 ggcattctgc atattgacat cagccgtggg acccagggtc ggccagaatt ctctcgaagc
     3541 gataaaggat attcttctcg aaggcacggc ggatgatctc gaaagttcac taaaaggtat
     3601 ccataagttc cccgaaaaag catattttat agttcacacc cgcaactatc tgaaaaagga
     3661 gtacggcttt aagttgaggg agctagtcga ttcgttcggc gaccccgaga ctcggaggga
     3721 ctttttcgcc acaaacaagg aaataaaagg gataggctac acgcaggcga gccactttct
     3781 gagaaacata ggattcggcg gttatgccat tctcgataag aacgtcgtta aatcgctcta
     3841 tgatctgggt ataatagact ccccgaaacc gcccgcgacg ggaaagaaat acaaggagat
     3901 agaagaaagg atgaaggccc tggccggaga gctcgggata ggactcgacg agctggacat
     3961 gctcctctgg agcctcaaga caggaaggat acctgtataa cgatggaaag ccgccgagag
     4021 gttggataac ctacagcttg atctcgatga atatttcagc gtactgcacg accgagaatc
     4081 cagccgtctc gttgaagacc gccctgtacc tgttgagtcc cctcacgccg tccgtgtaat
     4141 ccttgtactc cacctggctc ctgtgggcgt ct
//
LOCUS       DASSJH010000109         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_564059, whole genome shotgun sequence.
ACCESSION   DASSJH010000109 DASSJH010000000
VERSION     DASSJH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564059"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFJ67_02340"
     CDS             complement(<1..404)
                     /locus_tag="VFJ67_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289251.1"
                     /translation="MGFEESVNNLSGYSNDKYPVTTLYLKLGPRERENFGYKITFKNL
                     AKGLRDEIGKSGLPKEALESVESDLSKIQSFIDNQDALTECRGLALFSCSGGGFWEAL
                     KLPSLDRNQILIDRSPVLGQLFKAEDDHRHIVT"
     gene            980..1615
                     /locus_tag="VFJ67_02345"
     CDS             980..1615
                     /locus_tag="VFJ67_02345"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET7289252.1"
                     /translation="MNRYGVITGAFLILIAFLTVSMTGAGACADEIKAGKAAPDFTFT
                     DIEGTTTKLSDFRGKYVVIEWFNHGCPFTEKHYKSDKMQDLQRKYTEEGVVWISVNST
                     NEGHANYQDAAMTRKEAEAYGTSATYIVLDPSGKIGKLYGAKTTPDIFIVDPQGKLIY
                     AGAADSINSTDTYDIPKAENYIDLALTEAMAGTPVATPETKPYGCGVKYKE"
     gene            2071..>2434
                     /locus_tag="VFJ67_02350"
     CDS             2071..>2434
                     /locus_tag="VFJ67_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289253.1"
                     /translation="MDGTELFDRHLKEHTANGNIHPGENWYERDYREGDHILGGFTDL
                     DWHSLRKLFGTRTHIWQEACVYVLGESDNPGSAKMLADIFVRGQDNISCYSAIFLSEQ
                     NLDTFPDETKRLIRLRAHD"
BASE COUNT          617 a          557 c          698 g          562 t
ORIGIN      
        1 gtgacgatgt gcctgtgatc gtcttccgcc ttgaagagct ggcccagtac gggtgagcgg
       61 tctatcagta tctggttacg gtcgagcgag gggagcttga gagcttccca gaacccgccg
      121 cccgagcacg agaaaagggc caggcccctg cattcggtaa gcgcgtcctg attgtcgatg
      181 aagctctgga tcttcgagag gtctgattcg accgattcga gagcttcctt agggaggccg
      241 cttttcccta tttcatccct gagccccttg gccaggttct tgaacgtgat cttgtatccg
      301 aagttctccc gttcccgcgg tccgagcttt aaatatagtg tcgtaacggg atatttatcg
      361 ttcgagtatc cgctcaggtt gtttactgat tcttcaaatc ccatttcctt ctccttcgaa
      421 ttttgagttg agaggtcccg cgcataatcg ccgggcggag cataagagga taccagaaag
      481 cggggcgatt tcgaggggtt aagtttaggg agggggcagg gatatgtata agtccggagc
      541 tgggctgagt atctgttaac agggagaaaa gtgtttgaat tagattaatg gaagggggat
      601 gtatccggag ttactttgaa cggggaaagc gtcagagagg gaggagaaac tcctccctct
      661 ctgacgcacc gaaacttgta tggggtatgg gcaaccctga cacacgcccc ttcattcgag
      721 ggggactctg gcggagtgtg taaagaattc tgcttaccct atacacctga aatgatatac
      781 agaatatcta gaatgtcaat tattactgat tactaatgat aattaatgta tgcctgattg
      841 tttgggaagt tagtccggga ggaatccggg ccgtgtgaat tataatcctt gaagaatctc
      901 cattcgcatt ttcttaaccg cggctgcggg atgagcggtt atactttccg ttcaacaata
      961 gagatagagg aggcggggca tgaacagata cggagtcata accggggcgt ttctgattct
     1021 gatcgcgttt ttgacggtct ccatgaccgg ggcgggagct tgtgcggatg agataaaggc
     1081 cggaaaggct gcgcccgact ttacgttcac ggacattgaa ggcacgacga cgaagctttc
     1141 cgatttcagg ggcaagtacg tcgtgatcga atggttcaac catgggtgcc cgttcacgga
     1201 gaagcactac aagagcgaca agatgcagga cctacagagg aagtacaccg aagagggtgt
     1261 cgtatggata tcagtgaact cgacaaacga ggggcacgcg aattaccagg acgccgcgat
     1321 gacgaggaag gaggccgagg cttacgggac ctccgcgacg tatatcgttc tcgatccctc
     1381 ggggaagata gggaagctct acggggcgaa gaccacgcca gatatattta tcgttgatcc
     1441 gcaggggaag ctgatctatg cgggggccgc cgacagcatc aattcgacgg acacgtacga
     1501 tataccgaag gctgagaatt acatcgacct cgcgctcact gaggcgatgg cggggacgcc
     1561 cgtcgccacg cccgagacga agccctacgg gtgcggcgtg aagtataagg agtagggggg
     1621 aggagcgctc acacgtctgg gattgtgtgg atggcggagg gaaggacaac acaaaaggca
     1681 aatcttattt cttcctttcc ttgatgaaac acgcgtaatg tgtttgatga agatacgaag
     1741 aatggtgaaa agcatttgtt tcatccttct tttccttgat gaaaagaagc aaaaatcaca
     1801 cacgtagtgt gttttctgaa atgctcaaca taattcgcta aaaatcttat gtttaagcta
     1861 aaatctttcg aatgggaagc tgaaagattt ttttacgctt aaacgcgcga ttttctcaac
     1921 gcgaattctg taatgtcgga ggaagaaaaa tgctaaatcc taatatgttc gtattaatta
     1981 gccccggaaa ataaattgtg taaaataatc tccaataccc tgcgcggcgg gtgctgttaa
     2041 gcaaggcgcg cgggtgagga tcggcacggc atggacggca cggaactttt cgacaggcat
     2101 ctgaaagagc atacggccaa cggcaacata cacccgggcg agaactggta cgagcgggac
     2161 taccgggaag gggaccacat tctcggcgga ttcacggacc tcgactggca ctctctcagg
     2221 aagctgttcg ggacacggac ccacatctgg caggaggcgt gcgtctatgt gctcggagaa
     2281 tcggataacc cgggctcggc taagatgctc gccgatattt tcgtaagggg acaggacaac
     2341 atctcctgct actccgccat attcctctcc gagcaaaacc tggatacttt tcccgacgag
     2401 acgaagcggc ttatcaggct ccgcgcgcac gacc
//
LOCUS       DASSJH010000110         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1213630, whole genome shotgun sequence.
ACCESSION   DASSJH010000110 DASSJH010000000
VERSION     DASSJH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213630"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFJ67_02355"
     CDS             <1..321
                     /locus_tag="VFJ67_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7289254.1"
                     /translation="ISPLTPLYVLEEVLEYADMILIMTVEPGFPGQKFIQSMIPKVKR
                     LREIIDGSEHKPLIEVDGGIKLDNIRAIAEAGADVIVSGSGIFQSRSYAETIGAMRKE
                     LEGL"
     gene            418..2367
                     /locus_tag="VFJ67_02360"
     CDS             418..2367
                     /locus_tag="VFJ67_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7289255.1"
                     /translation="MVDKNRVPFRGVQENEAIRAILEGTSTETGRGFFVSLVDNLSKV
                     LNTHGAWVTEYLEEEGRLRSIACVMGGQRLKEFVYSVTGTACELVVHEKRLVHVREKL
                     FSEYKGNPDLKDFPKEGMVSYLGYPFVDVDGKILGHISVIDKDPIPEDLQVFNVFRIF
                     ANRGTAEMQRLRAEREILEREEKLRRLFDSAMDAIVEFDKNFKVTRINPAASRLFGYE
                     GERVLGRLVALLLNKESGVKLMKLTEFLDDKPEGEKFIWIPEGMRAVSGIGADFPAEA
                     TLSSYEMGKERYYTLILRNVDDKLQAEQKIRSLTRESEYLKEELKSLTESEGIIGDSE
                     SMLRVLADVKSVAGTDATVLILGETGTGKELIARSIHSSSRRSEKPFVKVNCPAIPAN
                     LIESELFGHERGAFTGATGKRDGRFSIADSGTIFLDEIGELSIDLQSKLLRVLQEGEF
                     EPVGSSKTVKVDVRVIAATNRDLLKEVEEGRFREDLYYRLNVFQIELPPLRERTGDIE
                     KLARAFMDKYAKRNGTVINPLSEEDVRRLESYSWPGNIRELQNVIERAVITSANGRLN
                     LERALPVSRDNTVPGSDPAAAADGTHVLSDRELKELEKKNIIRALEKAGWKVAGKDGA
                     AAILGIPTSTLNSKIKSFGIDITSR"
     gene            2561..2821
                     /locus_tag="VFJ67_02365"
     CDS             2561..2821
                     /locus_tag="VFJ67_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289256.1"
                     /translation="MKKLIAITVIGLSLGFSAVSFASDYMDLYTNGPAVSEVKSETNG
                     GEIETSPMSFYLSPVKVHVNETLTTVEETSDENTLLVFGVRI"
BASE COUNT          749 a          683 c          811 g          579 t
ORIGIN      
        1 ataagcccgc tgacgcctct gtacgtcctc gaagaggtcc tagagtacgc cgacatgata
       61 ctcatcatga cggtcgagcc cgggttcccg gggcagaagt tcatccagtc catgatacca
      121 aaggtaaaga ggctcagaga gattatagac ggctcggagc acaagcccct catagaggtg
      181 gacggcggca taaagctcga caacataagg gcaatcgccg aggccggtgc ggatgtcatc
      241 gtatcgggct caggcatttt ccagtcccgg agctacgccg aaaccatagg cgctatgagg
      301 aaagagttag aaggcctctg acggatgcct gttttaaaaa gccttccctg tacttgaaac
      361 ccgtattacg tatgtgcagc cgttgttgaa taatgagggg aaacttctat actcaatatg
      421 gtggataaga acagggtccc attccgggga gttcaggaaa acgaggccat cagggccatt
      481 ttagagggta cttccactga gaccggccgg ggatttttcg tctctctggt agataacctc
      541 tccaaggtgc tgaacacgca tggcgcctgg gttaccgagt atctggagga agaaggcagg
      601 ctcagatcca ttgcatgtgt aatgggcggc cagcgtctca aggaattcgt ttactccgtc
      661 acgggtactg cgtgcgaatt ggtggttcac gagaaacgcc tcgtgcacgt acgcgagaag
      721 ctattcagcg aatacaaggg caacccggac ctgaaagatt ttccgaaaga gggaatggtc
      781 agttacctcg gctacccgtt cgtggacgtg gacggaaaga tactaggaca tatatctgtc
      841 atagacaaag acccgattcc cgaagaccta caggttttca acgtattcag gatattcgcc
      901 aaccgcggga ccgcggagat gcagcgcctt cgcgcggaaa gagagatcct cgagagggaa
      961 gagaagctga gacggctttt cgacagcgcc atggacgcga tagtcgagtt cgacaagaac
     1021 ttcaaggtca ctaggatcaa tccggcagcg tcccgcctgt tcggatatga aggcgagagg
     1081 gttctgggca ggctggtagc tctcctgctc aataaggaaa gcggcgtgaa actcatgaag
     1141 ctcacggaat tccttgacga taaacccgaa ggggaaaaat ttatatggat acccgaagga
     1201 atgagggccg tgagcgggat cggcgccgat ttcccggccg aggctactct ctccagttat
     1261 gaaatgggca aagaacggta ttacacgcta atactgagaa acgttgacga caagctgcag
     1321 gcggagcaaa agataaggtc tctgaccagg gaatcggaat acctgaaaga ggagctgaag
     1381 tcccttaccg aatcggaggg cataatcggc gacagcgagt ccatgctgag ggttctcgcg
     1441 gatgtaaaga gtgtggcggg cacggatgcg accgttctga ttctcggcga gacggggacg
     1501 ggaaaggagc tgatcgccag gtcgatacac tcctcgagcc gccgctcgga aaagcctttc
     1561 gtaaaggtta actgcccggc cattcccgca aacctcatag agagcgagct cttcggacac
     1621 gagcgcggcg cgttcacggg ggctacgggc aagagagatg gtcgcttttc aatagctgac
     1681 agcggcacaa tttttctcga cgaaataggc gaactgtcaa ttgacctcca atccaagctt
     1741 ctgcgagtgc tgcaggaagg cgagttcgaa ccggtcggga gctcgaagac cgtaaaggtc
     1801 gatgtccgtg ttatagccgc gacgaaccgc gaccttctga aagaggtcga ggaagggcgg
     1861 ttcagagaag acctctatta caggctgaac gtatttcaga tagagcttcc cccgctcagg
     1921 gagcgaactg gagatataga gaagcttgcg cgcgcgttca tggataaata cgcgaagagg
     1981 aacgggaccg ttatcaatcc gctctcggag gaggacgtcc ggcgtctcga gtcttattcc
     2041 tggcccggaa atatacggga gcttcagaat gttattgagc gcgcggtaat aacatctgcc
     2101 aacggaagac tgaacctcga aagggcgctg cccgtgtccc gcgataatac ggtccccgga
     2161 tccgatccgg cagcggccgc cgacgggact catgtgcttt ccgatcgcga attaaaggag
     2221 ctcgaaaaaa agaacatcat ccgcgccctc gagaaggcag gctggaaggt cgccgggaaa
     2281 gacggcgccg cggctatcct gggtataccc acctcgactc tcaattcgaa gataaaatcg
     2341 ttcggcattg atattacttc cagataatcc ggcttgggaa ttattgcgag atcccgcagt
     2401 tttatagcga gaaatcacga gttgtgaaat cacgcaaaat ttatttaacc ggggattggc
     2461 ttctaaccct ctataattac aacataatca tgcgaaagta agagttggca cgcacgttgc
     2521 acttacacgt ccacaaataa atcaaacgga ggtaagtgca atgaagaagt taatcgcaat
     2581 tacggtaatc gggttatcac tcggtttttc ggctgtttca ttcgcttcgg actacatgga
     2641 cctttacacg aacggtcctg cggtcagcga agtaaagagt gagacgaatg gcggggaaat
     2701 agagacgagc ccgatgagct tctacctcag cccggtcaag gtacatgtca acgagacttt
     2761 gacaacggtt gaggagacga gcgatgagaa tactcttctc gtgttcggcg tcaggattta
     2821 ac
//
LOCUS       DASSJH010000111         3578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_675060, whole genome shotgun sequence.
ACCESSION   DASSJH010000111 DASSJH010000000
VERSION     DASSJH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675060"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VFJ67_02370"
     CDS             <1..926
                     /locus_tag="VFJ67_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET7289257.1"
                     /translation="AEITGDLDLKGEGTKVEIGGKVTVTKARITLPKQEEKQIEEIKY
                     VDEDKDEFVVGSGGQTDYFKENVALNLAIRMRKNNWVKGRGANIELKGDLDINKKYGQ
                     DVRIAGTITTVRGTYETLGKLFRIQEGTVNFTGAEKINPLLDITALYRVSSVQIYVNI
                     SGTAEKPVLKLTSNPDMTETDIISYIVFGAPSDQIGSGDRASIQGVATGVAGGIAAAQ
                     LEKLLGSKLSLDVISVGGGTSGPQLEVGKYLTQDLYIAYERETTESLIDSTTITENKV
                     LLEYTIFKNVTINGDVGGENPGVDVFYNFNY"
     gene            complement(939..1367)
                     /locus_tag="VFJ67_02375"
     CDS             complement(939..1367)
                     /locus_tag="VFJ67_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7289258.1"
                     /translation="MEPDRKKEVLKANSGFYQALRNGDLESMREVWLNEPEAKCVHPG
                     WPMLYGWEAVKESWKNIFQGGPPADIEISDVRVHVSGELAWVICIEKISQTTGGQTRH
                     GYAQATNVFELRGSSWRLVIHHASPVPVPAGDPGSNHNLQ"
     gene            complement(1424..2590)
                     /gene="queG"
                     /locus_tag="VFJ67_02380"
     CDS             complement(1424..2590)
                     /gene="queG"
                     /locus_tag="VFJ67_02380"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET7289259.1"
                     /translation="MDIAEITGSIKSKAHEVGFDLVGISPAGRFPESQFYKEWLGRGY
                     AGEMKYMETDPQKREDPGQIVQGARSVITCGLNYNTDYPYSTDAVEKDKGWIARYAWG
                     EDYHGLMDERLKALEEFISLSSAEGAHTRRYVDTGPVLERVYGKYGGIGWSGKNTCLI
                     NQRLGSWIFLGEIITTLELEYDSPAPDRCGTCTRCIEACPTGAITEPYVLDSRLCISY
                     LTIELKDEIPAGLRDKVGNNIFGCDICQDVCPWNRKAGKTAEPGFRPREGLYNPGLGS
                     FSGMGPDEFRARFKGSPVRRAKRKGFLRNVLTAMGNSGDKSLIPVIEEYASDSEPLVR
                     AHAQWALRKLGSGGSGDTPPGRIRAGADHIVSEDIGSLQGDEPDSHIQPVVKSR"
     gene            complement(2660..3130)
                     /locus_tag="VFJ67_02385"
     CDS             complement(2660..3130)
                     /locus_tag="VFJ67_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289260.1"
                     /translation="MKKLSGIKLVLVNADGFISDGEIGNGSHGPNGIHIRDLRGNDVE
                     FVAFSRSRDDLISSAAERLGIVLHHGISENSGFYSGIKDDYAVSDDEVAFICRDFTDI
                     PIMRLVSFTAATPDAPLDVKAESYFAAYTQGSGVVNEVAALILTAKKYPGGWSE"
     gene            3260..>3578
                     /locus_tag="VFJ67_02390"
     CDS             3260..>3578
                     /locus_tag="VFJ67_02390"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7289261.1"
                     /translation="MIPLRDTTRSPGFPYVNTALIITNVLIFLYGYLLGNYMDLFIFR
                     YGLIPANVFSSYTDADLADRIYPFLTSMFLHGGWLHLIGNMLFLYIFGDNVEGRMGHF
                     RYLV"
BASE COUNT          809 a         1023 c          940 g          806 t
ORIGIN      
        1 cggccgagat taccggagac ctcgacctca agggcgaggg cacgaaggtc gagatcggtg
       61 gcaaggtaac ggtaacgaaa gccaggatca ctctccccaa gcaagaggaa aaacaaatag
      121 aggagatcaa gtacgtagac gaggacaagg acgaattcgt cgtcggaagc ggggggcaga
      181 cagactattt taaagagaac gtcgctctta acctcgctat caggatgaga aagaacaact
      241 gggtcaaggg gcggggcgcg aacatagaac taaaaggtga cctcgacatc aacaagaagt
      301 acggtcagga cgtgaggatc gcgggcacga tcaccaccgt caggggcacg tacgagactc
      361 ttggtaagct tttcaggatc caggaaggga ccgtcaattt cacaggtgcc gaaaaaatca
      421 acccgctgct cgacataacg gcgctctacc gggtctcgag cgtacagata tacgtaaata
      481 taagcggaac ggcggaaaag ccggtgctca agcttacgag caaccccgac atgacggaga
      541 ccgatatcat ttcgtacata gtgttcggcg ctccctccga ccagatcggc tccggggaca
      601 gggcatcgat acagggtgtg gcgacagggg tcgcgggcgg gatagccgca gcgcagctcg
      661 agaagcttct cggaagcaag ctgtcgctcg acgtcataag cgtgggaggc ggcacaagcg
      721 gccctcagct ggaggtcggg aaatatctca cgcaagatct ctacatagcc tacgagagag
      781 agacaacgga gtcgctgatc gattcgacga cgattacaga aaacaaagtc ctgctcgaat
      841 atactatttt caagaacgta acgatcaacg gagacgtcgg aggggaaaac ccgggcgtcg
      901 atgtgttcta caacttcaat tactgattcc cgtgctgttt attgcagatt gtgattcgac
      961 cccggatcgc ctgcgggcac gggtacgggt gaagcatggt gtatcacgag ccgccacgat
     1021 gagcctcgca gctcgaaaac gttcgtcgcc tgtgcgtaac cgtgccgcgt ctgaccgcct
     1081 gtcgtctggc ttatcttctc aatacatata acccaggcga gctcacccga gacatgaacc
     1141 ctgacgtcgg aaatctcgat atcagcaggc ggccccccct ggaaaatgtt cttccagctc
     1201 tccttaacgg cctcccaccc atagagcata ggccagccgg gatgtacgca tttggcttca
     1261 ggctcgttga gccacacttc ccgcatcgat tcgaggtctc cgttacggag cgcctggtaa
     1321 aacccggagt tcgctttcaa cacctctttc ttacggtctg gctccattgg tcactcccga
     1381 cgcatttctc acttaattgc tcggcgcatt ctattttacc tatttaacgg ctcttaacca
     1441 caggctgtat atgcgagtcc ggctcatccc cctgaagaga gcctatatcc tcggacacaa
     1501 tgtgatccgc cccggccctg atacggcctg gaggcgtgtc gcctgatccc ccgcttccga
     1561 gcttacggag cgcccactgc gcgtgggccc ttacaagcgg ctcagagtcg gaggcgtatt
     1621 cttcgatgac cgggataagg ctcttgtccc cggaattgcc catagcggta agcacgttcc
     1681 tgagaaaacc cttcctcttg gccctcctta cggggcttcc cttaaacctc gccctgaact
     1741 catccggacc cataccggag aaggagccga gaccggggtt gtagagccct tccctcggcc
     1801 ggaagcccgg ctccgcagtc ttccccgctt ttctgttcca ggggcagacg tcctggcata
     1861 tgtcgcagcc gaatatatta ttgcctacct tgtccctcag cccggcgggt atctcgtcct
     1921 tgagctcgat cgtcagatac gatatacaaa gcctcgaatc gagcacgtag ggctccgtga
     1981 tagcgcccgt cggacacgcc tctatgcagc gtgtgcacgt gccgcatctg tcgggagccg
     2041 ggctgtcgta ttcgagctcg agggtggtga ttatctcgcc gaggaatatc catgagccga
     2101 gccgctggtt aattagacag gtgttcttcc cgctccaccc tataccgccg tacttcccgt
     2161 aaaccctttc gagcacgggc ccggtgtcca cgtaacggcg ggtgtgcgcg ccttccgccg
     2221 aggataggga gataaactcc tcgagcgcct tcagcctctc gtccatgagg ccgtggtaat
     2281 cctcccccca ggcataacgc gcaatccacc ctttgtcctt ctccacggcg tcggtcgagt
     2341 acgggtaatc ggtgttgtag ttaaggccgc acgtgatcac ggacctggcg ccctgaacta
     2401 tctgccccgg gtcttccctc ttttgagggt ctgtttccat gtatttcatc tccccggcgt
     2461 atcccctccc gagccactcc ttataaaact ggctctcggg aaatcgtccg gcgggagata
     2521 tgcccacgag atcgaacccg acctcatgag ccttcgattt gatggagccc gtaatttccg
     2581 cgatatccat tgactctaat tttatcacac cggcggtcca gggagttaat ccgcggaaag
     2641 cgtatagaag gttcggagac tactcggacc atccgcccgg gtatttttta gcagtcagga
     2701 tgagcgccgc gacctcgttc actactccgc ttccctgagt gtaagcggca aaataggatt
     2761 ccgctttgac atcgagcgga gcgtcaggtg ttgcggctgt gaagctgacc aatctcatga
     2821 tcggaatgtc cgtaaaatcc ctgcagatga aagcgacctc gtcgtcggag acggcgtagt
     2881 cgtcttttat tccggaatag aagccggaat tctctgaaat gccgtggtgg aggactatgc
     2941 caagccgttc ggcggctgaa gagataagat cgtctctgga tctggaaaat gcaacgaatt
     3001 ccacgtcgtt tcccctgaga tcccttatat gaattccgtt cggaccgtga gacccgttac
     3061 cgatctctcc gtccgatatg aagccgtcgg cgttgaccaa aaccagcttt ataccggaga
     3121 gcttcttcac aagatcagtt cttagttttc ggaacatggg ttttcagcct gcttaataat
     3181 aacacgccct caattttatg taaccggcgt gatattacaa gtggtgagag gcatacgccc
     3241 atgtataatc attatataaa tgatacccct ccgggatacg acgagatcac ctggcttccc
     3301 ctatgtgaac acggcgctca tcataacaaa cgtccttatt tttctgtacg gttacttgct
     3361 cggcaattac atggacctct ttatattccg ttacggactc ataccggcca atgtattttc
     3421 atcttatacg gacgcggacc tggccgaccg tatctatccg ttcctcacct ccatgttcct
     3481 tcacgggggc tggctccacc tgatcgggaa catgctgttc ctctatatat tcggcgacaa
     3541 cgtcgaaggc aggatggggc acttcaggta tctcgttt
//
LOCUS       DASSJH010000112         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_25734, whole genome shotgun sequence.
ACCESSION   DASSJH010000112 DASSJH010000000
VERSION     DASSJH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25734"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1115
                     /locus_tag="VFJ67_02395"
     CDS             126..1115
                     /locus_tag="VFJ67_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HET7289262.1"
                     /translation="MNKRVIWIFALAFVSLMYSACGGSKSDGTITLPQARRDVNPEAQ
                     ELNRKLIQESFSSDVAVDYLIGPGDLLSIEVLEAPDLNTEARVSTQNTISFPLLGKVE
                     VGGLTSQDAEERIRELLTEKYMNDPHVVVAIKEFRSQRVAVIGSVKNPGTYELLGKGN
                     LLDALALAGGLNDNASQIAYVTRKGKQGKEKSVQIDLNELLDEGKTDLNVPINMGDVV
                     YVPEAGVVYVDGAVNNPGTFKIQNDMTVSQAVTAAGGLSRTAEDSDVKLIRNKKGEME
                     VTSIDLEQIKEGNTQDIVLEDQDVVVVGKNAIRSFFDTIRLGLFFPPFSVGVQ"
     gene            1157..3538
                     /locus_tag="VFJ67_02400"
     CDS             1157..3538
                     /locus_tag="VFJ67_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET7289263.1"
                     /translation="MENGIRDNMNREEKGRNLVPDNEERALRSTYPHTPEILRAYPVD
                     EESPIHRYLEVVLRRKKIIIAFFAIVLISTLISTFMTQPEYRATAKLEISLENPKVVN
                     FDQVVELETKSEEFYETQYLLLKSNSLGKQVVDKINLAERQELEAKKNKPNIISRAMS
                     SLKSIIRSTKDGIKKMIVKPSPDSGASSASDVALAQAAKEDSLINSFLSGIQITPVGN
                     SRLVEVSYISKDRKFAAEAVNTLIDSYIEWILNRKLDATKQGRDFLRKQIEQAQSNLE
                     ESEEKLNAFAKSNDIVSLDEKMNITYHTFSELNDALAKAETARLEKESLYKHVLDGNI
                     DALTMVMNDPYVQNLKAEHAKAYSEYSQMSATFKPGYPKLKELGARVAELQRKIEEAQ
                     SSIATSIKSDYEASMKKEETLRESYQKQKTLASALNDKSIQYNILKREVDSNETIYNT
                     LLGRLKETEVSSAIKASNIQVVDYAQVPVIPFKPNVTLNMLFACIIGLGGGVFLAFAL
                     EFFDNTVKTSEEVRDKLKLPVLGGVFESNQPDSIGAPVEKSFLLDPRSHIAESFRTIR
                     TSLLLSTPGKPPRSILVTSCFPAEGKTTVSINLASSFAQAGSKVLLLEADLRRPRIGT
                     VLGNNGNGLSSYLTGNSDLSDVISQGEIPNLYVLPVGPIPINPAELLGSNRMRELIQT
                     LSAEYDYIIVDGPPALGFVDSHIISSIVDGVAVVIRAGKTPKNSIRELIDRLWSLKAN
                     FLGVIVNGIELNQNSYYYKSYNYYYGEEEQKKKLTAATKSSENKRQDDLGKSV"
     gene            3632..>4707
                     /locus_tag="VFJ67_02405"
     CDS             3632..>4707
                     /locus_tag="VFJ67_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7289264.1"
                     /translation="MKFTLNSRPSLYLSILFTIFVSLFLTWSSAKSWLSYVYAGDPPP
                     GGFTEAIQVEGDNSQLYFLLAQFYENYDFTAPRSDIYSLYRKALELNPLNYNYWYYLA
                     EFLSTEGKKDLALFSLDQATELAPGVVSLRWAAGILASRLGDEEALKTNLQAVIQFDS
                     ERRKKAFIVLWQSLRNGDKILPVIPEAAYDDYLNFLMDTDRIEEAQKVWDKLPNTDQI
                     PWYTFLRYVSILIDKGITKSAKQTWADKYGAWEGVWNGSFEKGLMNSGFDWTFNNAEG
                     ARIVLDETIKGRGNTVRVEFDGSSNIDFYNLRQNVPVEGNTGYELSAWMKSSDLPSRN
                     GVFWEAYCPRTKELYAKSEEVHGTT"
BASE COUNT         1333 a         1161 c         1269 g          944 t
ORIGIN      
        1 gccgattaag ggacttctcc ataacatcta gagcatttat ggaagctgat taatatcgct
       61 ataaatttta tgctattccg aaatggcggg tgtataatta atttataggt cgggaatggt
      121 gtattttgaa taaacgcgtt atatggattt ttgctcttgc attcgtttcg ctcatgtatt
      181 ccgcatgcgg cggctccaag tcagacggca cgatcacact gccgcaggcg agaagagacg
      241 ttaatccgga agcacaggag ctgaaccgca agctcataca ggagtctttc agctcggacg
      301 tagccgtgga ttatctgatc gggccgggag acctgctgtc gatagaggtg ctggaagccc
      361 cggatttaaa caccgaggcc cgcgtgagca ctcaaaacac gatttccttc ccgcttctgg
      421 gcaaggtgga ggtaggcggc cttacgtcac aggacgccga ggaaagaata agagagcttt
      481 tgacggaaaa atatatgaac gaccctcatg tggtcgtcgc cattaaggag ttcaggagtc
      541 agagggtggc cgtcatcggc agcgtcaaga accccggaac ttacgagctc ctgggaaaag
      601 ggaacctgct cgacgcgctc gccctggccg gagggttgaa cgacaacgcc agccagatcg
      661 catatgtaac gcgcaagggc aagcagggta aggaaaaatc ggtgcaaatc gacctgaacg
      721 agctactgga cgaaggcaag accgatctga acgtaccgat aaatatgggt gacgtcgtat
      781 acgtgcccga ggcgggcgtt gtatacgtgg acggagccgt aaataatccg gggacgttca
      841 aaatccagaa cgacatgacg gtcagccagg cggtcacggc ggccggaggg ctctcgagga
      901 cggctgagga ctcagatgtg aaactgataa gaaacaagaa gggagagatg gaggtaacgt
      961 ccatcgacct cgaacaaata aaagaaggga atacccagga tatagtgctc gaggaccagg
     1021 acgtcgtagt agtgggcaaa aacgctataa ggagcttctt cgacacaata agacttggac
     1081 tgttcttccc gccgttcagc gtcggcgtcc aatagcggga gcggtactaa aacgagttgg
     1141 gaagaaaata agcaaaatgg aaaacggcat cagggataat atgaacagag aagagaaggg
     1201 aagaaatctc gtacccgata acgaggaacg ggcgttacgc agcacatacc cccatacgcc
     1261 cgagatactg agggcttatc cggtcgacga ggaaagcccg atacaccgtt atctggaagt
     1321 cgtactcagg cggaagaaga taattatagc ctttttcgca atcgtcctta tatcgacact
     1381 tatatcgacc ttcatgacgc agcctgaata cagggctacg gccaagctcg agatatcgct
     1441 tgaaaacccg aaagtcgtca atttcgacca ggtcgtcgag ctcgaaacaa aatccgaaga
     1501 gttttacgag acacagtacc tcctgctcaa gagcaactcc ctgggaaagc aggtcgtcga
     1561 taagatcaac ctggccgaaa ggcaggagct cgaagcgaaa aagaacaagc cgaatatcat
     1621 ctcgcgggcc atgtcgagcc tgaagagtat cataagaagc acgaaggacg gcattaagaa
     1681 gatgattgtt aagccttccc ccgattcagg cgcttcgagc gcgtcagacg ttgcgctcgc
     1741 ccaggcggcc aaggaggaca gcctcataaa ctcgttcctc agcgggatcc agataacccc
     1801 ggtagggaac tccaggcttg tggaagtgag ctacatatct aaagaccgca aattcgcggc
     1861 cgaggcggta aatactttaa tcgattccta catagagtgg atactcaaca gaaaactcga
     1921 cgccacgaaa cagggaaggg atttcctgag aaaacagata gagcaggcgc agtccaacct
     1981 ggaagaatcc gaagagaagc tcaacgcatt cgccaagagc aacgacatcg tttcactcga
     2041 cgaaaaaatg aacatcactt accatacttt ttccgagctg aacgacgcat tggccaaggc
     2101 cgagactgcg agactggaga aggagtcgct ctacaaacac gtactggacg ggaacatcga
     2161 tgcgctgacc atggtaatga acgaccccta tgttcaaaac cttaaggcgg agcacgccaa
     2221 ggcgtattcc gagtacagcc agatgagcgc gacgttcaag ccgggttatc ctaagctgaa
     2281 agagctgggt gccagggtcg cggagctcca gagaaagatc gaggaagccc aatcgagcat
     2341 cgcaacctca attaaatcgg actatgaagc ctcaatgaag aaagaagaaa cgctcaggga
     2401 aagttatcag aagcaaaaga ccctcgcttc ggcgttgaac gacaagtcga tacagtacaa
     2461 catattaaag agagaagtag actcaaacga aactatttat aacacccttc tggggagact
     2521 gaaggaaacg gaggtttctt ccgctataaa ggcgagcaac atacaggtag tggattatgc
     2581 ccaggtcccc gtgattccgt tcaagccgaa cgtgacactg aatatgctct tcgcgtgtat
     2641 aatagggctt ggcggaggcg tattcctcgc ttttgccctc gagttcttcg acaacaccgt
     2701 caagacatcc gaagaggtaa gagacaaact gaagctgccc gttttaggcg gggtattcga
     2761 atcgaatcag ccggacagca tcggggcccc ggtggaaaaa tccttccttc tcgacccgag
     2821 gtcgcatatc gccgaatcgt tcaggaccat caggacttct cttctgcttt cgacccccgg
     2881 aaagccgccg cgctctatcc tcgtaacgag ctgcttcccg gcggagggaa aaaccactgt
     2941 gtcgattaac ctggcctcgt ccttcgcgca ggcgggaagc aaagtcctgc ttctcgaagc
     3001 ggatctcagg cgtcccagga tcgggacagt gctcgggaac aacgggaacg gattaagcag
     3061 ctatctgacc gggaattcgg acctgagcga cgtaatttct cagggcgaga taccgaatct
     3121 atatgtgctt cccgtgggcc cgatacccat caatccggca gagctcctgg gctcgaaccg
     3181 catgagggaa ttgatacaga ccttgtcagc ggaatacgat tacataatag tagacggccc
     3241 tccggcgctc ggtttcgtag attcacacat catatcgagc atcgtcgacg gggtcgcggt
     3301 cgtcatcagg gccggtaaaa caccgaagaa ttcgatccgg gaacttatag accggctgtg
     3361 gagtctgaag gcgaacttcc tcggggttat agtcaacggc atagagctca accagaacag
     3421 ctattactat aaatcgtata actattacta cggcgaggaa gaacagaaga aaaagctcac
     3481 tgcggcaaca aaatcctcgg agaacaagag acaggacgat ttaggtaaat ccgtttagca
     3541 ttattcgaaa cacggagata ttacagtgtc atagaatatc tccggctatc agtgtgctta
     3601 tccgttttct gcacgagact ctgatcattt tatgaaattt acgctaaact cgaggccctc
     3661 cctatatcta tcaatacttt ttacaatatt cgtctcgctg tttctcacat ggagctcggc
     3721 aaaaagctgg ctctcttatg tgtacgccgg tgatcctccg ccgggcggct ttaccgaagc
     3781 catacaagta gagggcgata actcccagct atacttcctg ctggcgcagt tttacgagaa
     3841 ttacgacttc accgctccgc gcagcgatat ctacagcctg tacaggaaag cgcttgagct
     3901 aaacccgctc aattacaatt actggtatta tctggccgag ttcctatcca cggaagggaa
     3961 aaaggacctg gcgttattct ctcttgatca ggctaccgag cttgctccgg gagtcgtatc
     4021 cctcaggtgg gcggccggaa ttctggcatc gagactcggc gacgaggaag cgctcaaaac
     4081 caacttgcag gcggttatcc agttcgattc cgaaaggcgg aagaaggctt tcatagtcct
     4141 atggcaatcg ctcaggaacg gagataagat actgcccgta atacccgagg cggcttacga
     4201 cgattacctg aatttcctta tggacaccga taggatcgag gaggcccaaa aagtctggga
     4261 caagctgccg aataccgacc aaatcccctg gtacacgttc ctcagatatg taagcattct
     4321 catcgacaag ggcatcacca agagcgctaa acaaacctgg gccgacaaat acggagcctg
     4381 ggagggtgtc tggaacggga gcttcgagaa gggacttatg aatagcggct tcgactggac
     4441 attcaacaac gccgaagggg ccaggatcgt gctggacgag acgattaaag gccggggaaa
     4501 tacggtgaga gtcgaattcg acgggagcag caacattgat ttttacaatc tccggcagaa
     4561 tgtcccggtc gaggggaaca ccgggtacga gctgagcgca tggatgaaaa gcagcgacct
     4621 gccgtcgaga aacggcgttt tctgggaggc ctactgtccc cggacaaaag agttgtacgc
     4681 caaatcggag gaggtgcacg gcacgac
//
LOCUS       DASSJH010000113         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_997536, whole genome shotgun sequence.
ACCESSION   DASSJH010000113 DASSJH010000000
VERSION     DASSJH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997536"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VFJ67_02410"
     CDS             <1..1285
                     /locus_tag="VFJ67_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289265.1"
                     /translation="SGTYELEPVTGLDIDAPPFWSYLIGTKRRALVIDAADTRLYSGL
                     EGIQISNWATHHNWDPYHFKTTSEPPEIIEEIGRKFGSKLLTRENYKSTYAQDLDIFR
                     KLLENVRKKGEMSRSLIDRDRFDLSLMIFAESHAANHQFWKYHPNNREKGAPDCELTH
                     AIRDVYKAIDKEIGIILERFENPNICIVSSVGMEDDYPNAALAEAFLRRFGYQVSPAP
                     GKVSLRPLDIARRLLPEQLRVALSRHMPREKREALVSDMFKKGTDWNRTRAFALPVSY
                     TSFIRVNLRGREPGGIVGEGDEYTALTDEIVNELYKLTDPATGEPAVLSAKKSTEIFN
                     SRPHENLPDIFVEWKPGSFMRSVRHPRAELTQKEPEFFRRSDHSSYGFFALAGPSVKS
                     SGRTDDIGVLDIAPTLMSLLEEPVPVNMTGRRLDV"
     gene            1360..2856
                     /locus_tag="VFJ67_02415"
     CDS             1360..2856
                     /locus_tag="VFJ67_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET7289266.1"
                     /translation="MNDLRRSRDHTKSTHHMLDGAVRVFLAESLLIPTGLVTTIFLTR
                     SLGPESYGIYGLAVGIVIWIEFSICNIFGGTSIKFIGEAENWEPIAIKVLRLYILIST
                     ASAAALWIFSGEIASLLGAPKLGSYLSLLAIDIPILVLSTAHRQILVGRGKFRERAMT
                     SSVRWISRMVLIVLLVEAGLSAEGAILGIIFSSLIELIINRSYARIPLLKSSDFPLRR
                     LLGYVTPLFFFAVGMRLYEKLDLLLVKALGGTADQAGIYVAAQNLSIIPSLFALSFSP
                     LLLSTLSKAAGGGSREEVSHLIRTSYRLVVLLIPFAAIISGCSEEIVSLIFGIEYSGS
                     APLLSILIFGSVATVFISVNYAVLTASGKPGLPATLAGPIVPISAVFYFLVIPRFGMI
                     GASYVYTILAWAGALATLTAVHYLWGVHPPARTVLRAILVSAAAYYAGDLWKTSGIFV
                     IIKILVVTASVLASYFVMRELTIKELRVLFSGLIYGIGSPGEGEGRIP"
     gene            3010..3891
                     /locus_tag="VFJ67_02420"
     CDS             3010..3891
                     /locus_tag="VFJ67_02420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7289267.1"
                     /translation="MIRYEPVFTIVIPTYGRPKQLKECLDSVSELSYPGDGFEVIVVD
                     DGSKTSLDEIVGAFRKRINIKLIRQDHAGPAAARNRGGDEAGGRYLAFTDDDCKPAKD
                     WLTRLEESLEPNSEILVAGRTINDLPENPYSTASQLIADFLYSYYNRNNAGFLTSNNM
                     ALSKKQFLKVGGFDITFPLAAAEDREFCDRYLYHGYKTLYVPEAVVRHAHPLTLGTFW
                     KQHFNYGKGAHRFHKIRSQRRQEPIKVEPVSFYVSLLLYPRNSTRRLSTALSFLIALS
                     QLANASGYFSAKYGPTG"
BASE COUNT          946 a         1114 c         1059 g          881 t
ORIGIN      
        1 gtccgggaca tacgagctcg aacccgtcac cgggctcgat atagatgccc ctcccttctg
       61 gtcttacctg atcggaacga aaaggagagc gctcgtcatc gacgccgctg acacgcgtct
      121 ttacagcgga ctcgagggca tacagatctc caactgggct acccaccata actgggatcc
      181 gtaccatttt aagaccacgt ccgagccgcc tgagataata gaggaaatcg ggcgtaaatt
      241 cggaagcaag ctcctgacca gagagaacta caaaagcact tatgcacaag acctcgatat
      301 cttcaggaag ctcctcgaga acgtgagaaa aaagggggag atgagccgat ccctcatcga
      361 ccgcgacagg ttcgaccttt cgctcatgat ttttgcggaa tcgcacgcgg ccaaccacca
      421 gttctggaaa taccacccga ataacaggga gaaaggcgcc ccggactgcg agctgacaca
      481 cgctatcagg gatgtataca aggcgatcga caaagagata ggcatcattc tcgagcgttt
      541 cgaaaacccg aacatctgta tcgtgtcctc ggtcggaatg gaagacgact atccgaacgc
      601 tgcgctcgcc gaagcgttcc tgaggaggtt cggataccag gtctctcccg cgcccggcaa
      661 ggtttcactt cgccctctcg acatcgcccg caggctgctg ccggaacagc tccgcgtcgc
      721 gctcagcagg cacatgccga gggagaagag agaagcgctc gtttcggaca tgttcaaaaa
      781 gggaacggat tggaaccgga caagagcatt tgcactaccc gtttcatata cgagcttcat
      841 acgcgttaac ctgagagggc gggagcccgg ggggatcgtc ggggaaggcg acgaatacac
      901 agcgctgacg gacgagatcg tcaatgagct ctacaagctg accgatcccg caacgggtga
      961 gcccgcagtg ctctccgcca aaaaatcaac cgagattttt aattcccggc cccacgagaa
     1021 tctcccggat atattcgtcg agtggaaacc gggaagtttc atgcgcagcg tgcgccaccc
     1081 ccgggccgag ctaacccaga aggagcctga attcttcagg aggagtgacc attcgtctta
     1141 cggttttttc gcgctcgccg gcccttccgt gaaatcgagc gggagaacgg acgatatcgg
     1201 cgtgctggac atcgccccga ccttgatgtc gcttctggaa gagcccgtac cggtaaacat
     1261 gacggggcgg agattggacg tttagccgca tcacgttccg ctcccgggga ttgcagcagg
     1321 cactagcccc ctttacagat cacgaataca atactcacaa tgaacgacct gaggcgcagt
     1381 agagatcaca cgaagagcac tcaccacatg ctcgacggcg ctgtccgcgt cttccttgcg
     1441 gaatcgctgc tcatacccac gggactcgta acgactatct tccttacccg cagcctcggg
     1501 cccgagagct acggaatcta cgggcttgcg gtcggcatcg tcatctggat cgagttcagc
     1561 atctgcaaca tattcggagg gaccagcatc aagttcatcg gcgaggccga gaattgggag
     1621 cctatcgcta taaaagtcct caggctatat atattgatca gtacggcctc ggccgctgcg
     1681 ttgtggattt tttcaggcga gatagcatcc ctcctgggcg cgcccaaact cgggtcatac
     1741 ctttcgcttc ttgcgatcga tattcctatt ttagtcctgt ccacagccca ccggcagatt
     1801 ttggtaggca gagggaagtt cagggaacgg gcaatgacaa gctcggtgag gtggataagc
     1861 cgcatggtgc tgatcgtgct gctggtcgaa gcgggtctct cggcggaggg ggcaatcctg
     1921 gggataatct tttcttccct gatagaatta ataatcaacc gctcgtacgc ccgtattcct
     1981 ctactgaaaa gttcggattt cccgctgaga aggttattgg ggtacgttac acctctcttt
     2041 ttcttcgccg tgggaatgag gttgtatgaa aaactcgatt tgcttctcgt aaaggccctc
     2101 ggcggcacag ccgatcaggc cggcatatac gtggcggcgc agaacctctc tataatcccc
     2161 agcctgttcg ctctctcgtt ctccccgctc cttctctcga ccctgagcaa agccgccggc
     2221 ggcggcagca gggaggaagt ttcccaccta ataaggacct cttatcgcct cgttgtgctt
     2281 ctgatccctt tcgccgcaat tatttccgga tgctctgagg agatcgtgag cctcatattc
     2341 ggcatcgagt acagcggctc ggccccgctg ctgtcaattc tgattttcgg ctctgttgcg
     2401 acagtcttca tatccgtaaa ctatgccgtg ctcacagcca gcggcaagcc gggtcttccg
     2461 gcgactctag cgggacccat agtgcccatt tcagccgtat tctattttct cgtaatcccc
     2521 cgcttcggca tgattggggc ttcatacgtc tatacgatac ttgcctgggc cggcgctctc
     2581 gcaaccctga cggcggttca ctatctgtgg ggagtccatc ctcccgcgag gaccgtactg
     2641 agagcgatac ttgtatcggc cgcggcatac tatgccggag atctgtggaa gacgtccggt
     2701 attttcgtta ttataaagat actggtcgtg acagcgtccg tgctcgcctc atacttcgtt
     2761 atgagagagc tcactatcaa agagcttcgg gtgctgtttt cgggattaat atacggcatc
     2821 ggaagcccgg gcgaaggaga aggccggata ccgtgacggg gcggcaattc ttagggagtg
     2881 aatataaaat cagtacgttt cgaattcgcc gacgggtatg ttaatcaaga gagaagcgaa
     2941 tatgcacaca gcccgaaggg tgctgccggt tattttcaat gatgatagaa agtcattagc
     3001 atttcatata tgatccgata cgaacctgtc ttcactatcg tcatacccac atacgggcgt
     3061 ccgaaacaac tgaaggagtg tctcgattcg gtatcagagc tctcgtatcc cggggacggt
     3121 ttcgaggtca tagtggtcga cgacggcagc aagacctcgc tcgacgaaat cgtcggcgcc
     3181 ttcaggaaaa ggataaacat aaaactaatc agacaggatc acgccggtcc cgcagctgcg
     3241 cgcaacaggg gcggagatga agcgggagga aggtacctcg cgtttaccga cgacgactgc
     3301 aaacccgcga aggactggct gaccagactc gaagaatcgc tagaaccgaa ttccgagatt
     3361 ttagtcgcgg gacggacaat taatgattta ccggaaaacc cgtattcgac cgcgagccag
     3421 cttatcgccg attttctcta ttcttactat aatagaaaca acgccgggtt tttgacctcg
     3481 aacaatatgg ctctctcgaa aaaacagttc cttaaagtcg ggggattcga catcaccttc
     3541 cccctggccg ccgccgagga cagggaattc tgcgacaggt atctatatca cggatataag
     3601 acgctttacg tgcctgaggc cgtcgtgcgt cacgcgcacc cgctgacgtt aggcacgttc
     3661 tggaaacagc attttaatta cggaaagggc gctcacaggt tccataaaat ccggtcgcag
     3721 cggcgtcagg aaccgattaa ggtcgagccc gtcagctttt acgtttccct actgctttat
     3781 cccaggaatt cgacccgccg gttgagtacc gccctttcat ttttgatcgc gctgtcccag
     3841 ctcgcaaacg catcgggtta cttctcggca aagtacggtc caacgggata gcatctcaat
     3901 ggcgcacatc gtacgcgacg ctccgcatac tattctagac cgcccgtctg ccgaatatca
     3961 gcgagactat aaagatgatc aaaaatacta cgaacaatat
//
LOCUS       DASSJH010000114         5175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_26130, whole genome shotgun sequence.
ACCESSION   DASSJH010000114 DASSJH010000000
VERSION     DASSJH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26130"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFJ67_02425"
     CDS             complement(<1..632)
                     /locus_tag="VFJ67_02425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008186673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET7289268.1"
                     /translation="MSPENPLVSVIVPFLNAERFIRETIESVLSQTYKGWELLLVDDG
                     STDDSTEIALQYEKENRGKVRYLEHDKHLNLGLPASRNLGLRNCKGKYIALLDADDVW
                     FPNKLEEQVAILESEAEAAMVYGVSEYWYSWMGETHDKQSDYKPELGVKPSTLVNPPE
                     LLTLALRSEAPTPCPSDILVRRDILVEIGGFEEEFSGIYQLYEDQAFLSK"
     gene            complement(768..2015)
                     /locus_tag="VFJ67_02430"
     CDS             complement(768..2015)
                     /locus_tag="VFJ67_02430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7289269.1"
                     /translation="MPERLRFCMITTFYPPLSFGGDAEYVWQLSNELARRGHHVTVIH
                     CVDSYEALSGNRSYAPYGNHPNVEVHGLKSGYGFLSPLATQQTGYPLFKSERIREILS
                     GGFDVTHFHNISLIGGPKVIEYGSGIKLYTTHEYWLLCPTHVLMRYNTAPCVKPRCFA
                     CQLVHGRPPQLWRYTDLMKNSLSHLDAIISPSKFTKKIHEESGLTTPIFHIPHFAPLP
                     AETPGQDGKGIEASRGKPYFLYVGRIEKLKGIQTLIPLFRGFGKADLLIAGKGEYESR
                     VRKLAEGAENIHFLGHLSRDRLGKLYENAAALIVPSIAYEISTLVVFEAFREKTPVIA
                     NDIGGLPELIEESGGGFVYGSERELLSAMDRLLEEPGLRDELGRKGHEKYLEKWSAEF
                     HIGAYLELIDGLMSKNGSRGNHP"
     gene            complement(2021..2812)
                     /locus_tag="VFJ67_02435"
     CDS             complement(2021..2812)
                     /locus_tag="VFJ67_02435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7289270.1"
                     /translation="MSACNFEERWIIIAGNIPVLTFHAIEDGPSVISFSPELFERFMR
                     KLHRSGYRTVRLSDAAGLVRERKPFPEKSFVLTFDDGYRSVYGGAFPVLRKYRMTATV
                     FLTVGDSEIRGPQDRLPTLGGRQMLSWGEIIEMKDAGIDFGAHTVTHPDLTAIDAARM
                     EYEIRGSKEIIENNLGEPVLSFAYPYGKYDGRSLDIAGRYFGCACSDDLGILTISSAL
                     YALERVDAYYLRQERLFGLMLTKSFPLYVFLRNIPRTIVRRLGGR"
     gene            complement(2879..4456)
                     /locus_tag="VFJ67_02440"
     CDS             complement(2879..4456)
                     /locus_tag="VFJ67_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289271.1"
                     /translation="MLDGIKMAARYAWGLNGYLLNTLTPAECRLMIGNQLRSRGHSFM
                     SIAERGIYSNPKSPYLRLLRHAGIEYGDLAALVRESGVEGSLERLYDAGIHVRLDEFK
                     RRVPISRPGLEFAPGPHDFDNPLLSGQYSSRTSGSRGGVTRVIMDLDLLEHDAACHQF
                     MLEAFEVGERPFGIWREVPPVTTGMNILLRLTKLGKKVEKWFSTSRVGVSPGSLQFAF
                     FTYYTILASRVLRRPLPAPEYTPLEDVAVAARWLAGKKSDGEPAFFDTQASSAVKVCI
                     AAGEEGLDISGTFFCIGGEPYTKGKADVITGAGCRAIPRYSMAELGNIGMGCAAPEEL
                     DDVHLLTDKIAVIQRRKSVGEGELDVGALVYTTILPSSPKLMLNVESDDYGVLEERNC
                     GCAIGEAGFTKHLSGIRSYEKLTSGGVTFLGTELLRLIEEVLPRRFGGHPTDYQLAEE
                     EEGGLPRVNIVVSPRVGNVDEETVVNTVYQALLPYPGGDVMSERWQQGRMLRVVRREP
                     YTTASAKILPLHILKAK"
     gene            complement(4629..>5175)
                     /locus_tag="VFJ67_02445"
     CDS             complement(4629..>5175)
                     /locus_tag="VFJ67_02445"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET7289272.1"
                     /translation="KHLGFIPVFSASVPHGLRFNESWAKHDDAPPGYFRGSQLHHIDY
                     HDRPMAYVIVLLNDITMDNGPFCFLPESSSRKATKALNYRKRGKPHRLSDQEVYSAIS
                     ESELVKVCYPEGTVLFFDPSVCFHYGSRNAVNPRYALMYAFVSPCRTDFTEVLLERKL
                     YPVRDNDSRLRKMVLRREYTG"
BASE COUNT         1079 a         1559 c         1353 g         1184 t
ORIGIN      
        1 attttggaca aaaacgcctg atcttcatac aactggtata tgccggagaa ctcctcttca
       61 aaacccccta tttctaccag tatatcgcga cgcaccagta tatcggacgg gcagggtgtc
      121 ggagcttccg acctcagtgc cagtgtcaac aattcaggcg ggttcacgag tgtgcttggt
      181 ttaacaccga gttcaggttt atagtcgctt tgcttgtcat gcgtctctcc catccagcta
      241 taccaatatt cggaaacgcc gtataccatc gccgcttcgg cttcggactc caatatagca
      301 acctgttctt ccagtttatt gggaaaccac acatcgtcgg catcgagcag cgcaatatat
      361 ttccccttgc agtttcgcag gcccaaatta cgcgatgcgg ggagcccgag atttaaatgc
      421 ttgtcatgtt cgagataacg aaccttaccg cgattttctt tctcgtattg aagagctatt
      481 tcagtgctgt catcggtaga gccgtcgtcg acgagcaaaa gctcccatcc tttataagtt
      541 tgagagagca cggactctat cgtctctcgt ataaacctct cagcattcag aaagggaaca
      601 atgacggata caagcggatt ttccggactc atgccacgaa tgtttcaaaa acgatattat
      661 tgtttcatat acatattata agttacctgt ataatgacat tatttgcgcc atcatgtcaa
      721 ttccgcaccc cgtccatcgc ccggtcataa cgaagcggct ccccgcccta cgggtgattg
      781 ccgcgagacc cgttcttcga catcaatccg tctatcaact ccaggtacgc tccgatatgg
      841 aattcggcgc tccatttctc gaggtacttt tcatggccct tccttccgag ctcgtccctc
      901 agcccgggct cctcaaggag cctgtccatc gcgctcagaa gctcccgctc cgagccatac
      961 acgaaccctc cgccgctctc ctctataagc tccggcagcc cgccgatgtc gtttgcgata
     1021 acgggcgtct tctccctgaa cgcctcgaat accacaaggg tcgagatctc gtacgcgatc
     1081 gacggtacga tcagggccgc cgcattttca tagagcttcc cgagacggtc gcgggacaaa
     1141 tgtccgagga agtgtatatt ctccgcccct tcggcgagct ttcgcacccg gctctcatac
     1201 tcccccttac cggcgatcag gaggtccgcc ttcccgaatc ccctgaacaa gggaatgagt
     1261 gtttgaatgc cctttagctt ctcgatcctg ccgacgtaga ggaagtaagg tttcccgcgc
     1321 gaggcttcga tccccttccc gtcttgtccc ggagtttcag cggggagggg ggcgaagtgg
     1381 ggaatatgaa agatgggcgt ggtcaaaccc gattcctcgt gaatcttctt tgtgaatttg
     1441 ctcgggctta ttatcgcgtc cagatggctc agagaatttt tcatcagatc cgtgtaccgc
     1501 catagctgcg gcgggcgccc gtgcacaagc tggcacgcga agcaccgggg cttgacgcac
     1561 ggtgcagtgt tgtatctcat cagaacgtgc gtcgggcaga ggagccagta ctcgtgagtc
     1621 gtgtagagct tgatcccgct cccgtattcg atcaccttcg gccccccgat gagcgagatg
     1681 ttgtggaagt gggtgacatc gaacccgccc gagaggatct cccgtatccg ttccgatttg
     1741 aagagcgggt atccggtctg ctgcgtcgcg agaggggaca ggaacccgta tccgcttttg
     1801 agcccgtgga cttccacatt ggggtggttc ccgtacgggg cgtagctcct atttccggag
     1861 agcgcttcgt atgaatcgac gcagtggatc accgttacat gatgacccct ccttgcgagc
     1921 tcgttcgaaa gctgccagac gtattccgcg tcgcccccga agctcagcgg agggtagaac
     1981 gtcgttatca tgcagaacct gagccgctcc ggcatggcgc tcatctcccg cccagccttc
     2041 gcactatcgt tctcggtata ttgcggagaa agacatacag gggaaaagat ttcgtcagca
     2101 taagtccgaa gagcctctcc tgcctgagat aataggcgtc cacgcgctca agagcgtaaa
     2161 gcgcgctcga tatcgtcaag atgccgaggt cgtccgagca ggcgcacccg aagtatcttc
     2221 cggctatatc gaggctcctc ccatcgtact tcccgtacgg gtaggcaaac gataaaaccg
     2281 gctcgcccag attattctcg attatctctt tagaccccct tatctcgtat tccatgcggg
     2341 ccgcgtcgat cgcggtaagg tcggggtgcg taacggtatg ggccccgaaa tcgatcccgg
     2401 cgtctttcat ctctattatc tctccccagc tcagcatctg cctcccgccg agagtcggca
     2461 gacggtcctg cggcccccta atctccgagt cgccgaccgt aaggaacacg gtcgcggtca
     2521 tcctgtattt cctcaacacc gggaatgccc cgccatacac gctcctgtac ccgtcgtcga
     2581 acgtcaggac gaacgatttt tccgggaagg gtttcctctc gcgcacgagc ccggcggcat
     2641 cgctcagcct gaccgtcctg tatccgctcc ggtggagttt cctcatgaag cgttcgaaca
     2701 gctccgggct gaaagagatg acagacgggc cgtcctctat agcatgaaag gtcaggacgg
     2761 gaatgttgcc cgcgatgatt atccacctct cttcaaaatt acaagcagac aatagtatct
     2821 aatagagaac aagcaaaggc aataattacg acggggacgg ggctttttct tgctagcctc
     2881 actttgcctt gaggatgtgg agggggagta ttttagcgga ggccgtggta tagggctccc
     2941 tcctgacgac gcgcagcatg cgtccctgct gccatcgctc cgacatgaca tcgcctcccg
     3001 gatagggaag caaggcctga tacacggtat taacgacggt ttcctcatcc acattgccca
     3061 ccctcgggct cacgactata ttcacacggg gcagtccccc ctcttcttct tcagcgagct
     3121 gatagtccgt agggtgcccc ccgaacctcc gcggcagcac ctcttctatg agcctcaata
     3181 attcagtccc caggaacgtc actccgccgc tcgtcagctt ctcgtagctg cgtatgcccg
     3241 agaggtgttt cgtgaatccg gcctccccga tcgcgcaccc gcagtttctt tcttcgagca
     3301 ccccgtagtc gtcgctctcc acgttcagca tgagcttggg agagctcggc agtattgtcg
     3361 tatagacgag ggcccctacg tcgagctcgc cttcaccgac ggattttcgg cgctgtatca
     3421 cggctatctt gtccgtcagc aggtgcacgt cgtcgagctc ctcgggcgcg gcacatccca
     3481 tgcctatatt gccgagctcg gccatggaat aacggggtat ggccctgcac ccggccccgg
     3541 ttatcacgtc cgccttgccc tttgtataag gctccccgcc gatacagaaa aaggtcccgg
     3601 atatgtcgag gccttcttcc cctgcggcta tacagacctt cacggccgag ctcgcctgcg
     3661 tgtcgaaaaa tgcgggttcg ccatccgact ttttccccgc gagccagcgc gcggctacgg
     3721 ccacgtcctc aagaggcgta tactcgggcg ccggtaacgg cctccgcaac accctgctcg
     3781 cgagaattgt atagtaggtg aagaaagcga actggagcga tccgggagac acaccgaccc
     3841 tgctcgtaga gaaccacttt tccacttttt tccccagctt cgtcaggcgg agcagaatgt
     3901 tcatgcccgt agtcacggga gggacctctc gccagatacc gaacggccgc tctcccacct
     3961 cgaacgcttc gagcataaat tgatgacacg cggcgtcgtg ctcgagcagg tcgaggtcca
     4021 ttattacgcg cgtcacgccg ccgcgcgagc cgctcgtccg cgagctgtac tgcccgctga
     4081 gtaacgggtt gtcgaaatcg tgaggccccg gagcgaattc cagtcccgga cggcttatcg
     4141 ggacccgcct cttgaactcg tcgagacgga cgtgtatccc cgcgtcatag agacgctcga
     4201 gactcccctc gacacccgac tcacggacga gcgccgcaag gtctccgtat tcaatgcccg
     4261 cgtgcctgag gagcctcaag taagggcttt taggattgga ataaattcct ctctcggcga
     4321 tgctcatgaa cgaatgcccc ctcgacctca attgattccc gatcatcagc cggcattcgg
     4381 ccggggtaag cgtattaagg agatagccgt tcagacccca ggcgtatctc gcagccatct
     4441 ttatgccgtc cagcatcggt tatccacgtc gcggagatgt cgagtcccgt ccaataatat
     4501 cccggaattt atactgatgt caggcgacaa gccggcgttc ttcaagcttg atttaaatat
     4561 cgagtcgtgc ccgcgcattg ccccacgagc cgtcacgtca gccgtgcggc aagacgcggt
     4621 atgaggcgtt aaccggtata ttctctcctg aggaccattt ttctgagccg ggaatcgtta
     4681 tcgcggacgg gatataattt cctttcgagg agcacctctg tgaaatccgt cctgcacgga
     4741 gaaacgaacg cgtacatgag cgcgtagcgc gggttcaccg cgttcctgct cccgtaatgg
     4801 aaacaaacgc ttggatcgaa gaagagaacc gtcccctcgg ggtaacacac cttgacgagc
     4861 tcgctttcgg aaatagccga atacacctct tgatccgata agcgatgcgg ctttcctctc
     4921 ttcctgtaat tgagggcttt tgtcgccttc cgggaactgg attccggcag gaagcagaaa
     4981 ggtccgttat ccatcgttat atcgttgagc agaacgatca cgtacgccat aggcctgtcg
     5041 tggtaatcaa tatgatggag ctggctcccc ctgaaatatc cgggcggagc gtcgtcgtgc
     5101 ttggcccagg attcgttgaa cctcaagccg tgcggaacgg atgccgagaa gacggggata
     5161 aagcccaggt gcttg
//
LOCUS       DASSJH010000115         8214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_459730, whole genome shotgun sequence.
ACCESSION   DASSJH010000115 DASSJH010000000
VERSION     DASSJH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459730"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFJ67_02450"
     CDS             complement(<1..730)
                     /locus_tag="VFJ67_02450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET7289273.1"
                     /translation="MTESPLISCLCITHGRTNLLGRAVRSFRDQTYPNKELVLLYESG
                     DGETAEYVRTLEDRNLKLVEIPSSEEFNLGKLRNRSIRECSGEYFCQWDDDDWSHRER
                     LSFQMEVIKASRLPACVLFHWLMYDERTGKAYLSNRRPWEGSLLCKTSLLGGDLIYEE
                     VVRHEDTRLVMELFRRSLVFPVMMPKLYIYVYHGHNLCTREHWEKIFRASVELSAEST
                     SIIRGILDGAYSGEEASRLLDGIEE"
     gene            complement(741..1844)
                     /locus_tag="VFJ67_02455"
     CDS             complement(741..1844)
                     /locus_tag="VFJ67_02455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7289274.1"
                     /translation="MSDIIFSASMIVRNEEKFLEGCLESIKDVVDEIVIVDTGSTDAT
                     REIAARYGARVFDFPWTGNFSAARNRALEHCRGGWILYIDADERLRPVSREEVRRVLS
                     DTSNAAYTVRFHPIGGYTAYKEYRIFRNDPRIRFYSRIHETIFPSIEKFMEQEGLGIG
                     DSALAIDHTGYDGELTHKHERNLPLLRSQIERDPGRMYLRWQLGVAQKGLGDMEGAER
                     TWMEAIDISRAKEAVTHEDSHAYYEMICLVNEKGGDPSGLLDEALSLFPDNYLLIWTK
                     AVTLARSRRFEEAVSVLEKLVSIDPDMLTDGPLSYNTAIFRELSYEPLADCLFQLGRR
                     GESLEYYTLASERCPDNEEYRTKRRFLSALVGK"
     gene            complement(2019..2636)
                     /locus_tag="VFJ67_02460"
     CDS             complement(2019..2636)
                     /locus_tag="VFJ67_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289275.1"
                     /translation="MSDKKFPDGINPERREFLEKVTKGAFIIPTVISVMMLNQKLNLS
                     TANAQSNDGTVCLSFGTMISTPLGNIPVFALKPGMLVYTLDADGVRTAMPLELVSKTS
                     VPSTHTICRLVLDDGREVRVSAGHPTADERKIGDIVPGDILDGAVVVSAEKVPYSAGF
                     TYDLLPSGDTGYYWADGILMGSTLSPGFGFIVRRPERQEELPLIA"
     gene            complement(2676..3422)
                     /locus_tag="VFJ67_02465"
     CDS             complement(2676..3422)
                     /locus_tag="VFJ67_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289276.1"
                     /translation="MHEAEQDYPVTKESVYDLNVSGGACKILKNDEAVHSSDDLSKTI
                     FNLQWLMHREALAGITDRIRIHAGCAEWEGKRFLVVGDKGAGKTTLMITLLLAGFRVV
                     GDELVLVKDGKAMPFPRRFNIKEESTHLLTQLKLLFETLPYNMTYYNHRMYSLSPHDV
                     GREWKIDEGEVRAVFYLVPNHGGETRIEECPKYMMVNRVMPMSFLSEKDDHMKISHLC
                     RMIDNAECHTIYIGGLEGAVSGLQERLSVI"
     gene            complement(3509..3766)
                     /locus_tag="VFJ67_02470"
     CDS             complement(3509..3766)
                     /locus_tag="VFJ67_02470"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HET7289277.1"
                     /translation="MSEYPKIAEGIDINEVEDGYVIYQSEKDKVHYLNKTAVLVLEAC
                     TGKNSTADITAIVKDAYGLDAAPEKEVADCLDTLIQEGLVK"
     gene            complement(3946..4101)
                     /locus_tag="VFJ67_02475"
     CDS             complement(3946..4101)
                     /locus_tag="VFJ67_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289278.1"
                     /translation="MKKILFTLAITMGIALGGAVLLTTNVLACGGKDKDTTSEQPTGS
                     GSDSTQS"
     gene            complement(4220..5056)
                     /locus_tag="VFJ67_02480"
     CDS             complement(4220..5056)
                     /locus_tag="VFJ67_02480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7289279.1"
                     /translation="MAETELKKEYALSDTENEKKRLEQQSRIIIETTRRIFELAGIVR
                     GMRVLELGTGAGDVALLLAQMVGHEGEVVSIERNPEILETAKKRAAQMGYGNIRFVNG
                     DISAAEPEGVFDALAGRAVLMYVPRPVEVLKALVRHVKPGGIVVFQEVDFTVRPVSIP
                     AYPLFMKCWGWLYAAMEKAGTEMQMGFKLLPVFRAAGLPEPVMHMDAFVGGAASAGLD
                     WFVSTIRSLVPMMVKHGVASAEEVGIDTLRERIIAEAAGSDALDWVGMSNTWISAWTR
                     KP"
     gene            5340..5804
                     /locus_tag="VFJ67_02485"
     CDS             5340..5804
                     /locus_tag="VFJ67_02485"
                     /inference="COORDINATES: protein motif:HMM:NF037752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7289280.1"
                     /translation="MRTLKFFLLLAILSVSNHAFAQIPECPCDTLVLQNGTTGNEIVE
                     LLCPGGELAENVLSVVHPNAVVIGDENGGYAVESIGGAGFACLIADEGGGLDGIQITE
                     EQFTDCRERLIQGCNLSFSLPIPTLSEWGMITMAGVLGIIGLILARRRKVVA"
     gene            6220..6876
                     /gene="sigZ"
                     /locus_tag="VFJ67_02490"
     CDS             6220..6876
                     /gene="sigZ"
                     /locus_tag="VFJ67_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigZ"
                     /protein_id="HET7289281.1"
                     /translation="MGRNIYNPIPVELVRVVSKQIGETENYMDTNSAADAGQIWEEFS
                     GRVRSHLLKKIRNKGDVDDLMQDIFMKIHAHLPHLRERDKLSSWIYRIAGNTLNDYYR
                     KGPGRETEFDENKTADGNADGDDPLSGIEGCLEAFIARLPEKYREPLVMSDVEGKSQK
                     EIAEEMDLSYSGLKSRVQRGREMIKDMFIECCKLSVGKDGKLKGELGNMDDCTLCGRG
                     "
     gene            7136..7573
                     /locus_tag="VFJ67_02495"
     CDS             7136..7573
                     /locus_tag="VFJ67_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289282.1"
                     /translation="MAGIPLFLKSIKSNNFLGIIFIAALLFSACDEGGGGDCVRIQDD
                     PDFLQDFVECPTDGLQIVCNRFDCVLSFPIDDPVPPPEQLVRINPRKCEAIDCFNMEC
                     GMKNPFTGEDLGSAVFSIEEFPGDGTFAGTAGNQDFTCSPVVE"
     gene            7762..>8214
                     /locus_tag="VFJ67_02500"
     CDS             7762..>8214
                     /locus_tag="VFJ67_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289283.1"
                     /translation="MSGNRKLRKSLRLDFRYENFNILLESQTRVEILPPPSDQLEEMD
                     EYNGYYVKLTDLENNVFYFQKLQNPLRTRTESYGDDAESQFTYETLKDPDPTFSILIP
                     DPGIVFTLDIINNPPQPELFFKIARQSFTFTIDPAEIILPPMPVAERVE"
BASE COUNT         1800 a         2274 c         2171 g         1969 t
ORIGIN      
        1 attcctcgat cccgtccaga agccgcgatg cctcttcccc gctgtacgcg ccgtcgagta
       61 tgcctctgat tatcgaagtc gattccgccg aaagctcgac cgatgcgcgg aatattttct
      121 cccagtgctc cctagtacag aggttgtgac cgtgatagac gtatatatac agcttcggca
      181 tcataaccgg aaaaacgaga ctccgcctga agagctccat tacgaggcgc gtatcctcat
      241 gccgcactac ttcctcgtat atcagatcgc cgccgaggag cgatgtcttg cagaggagcg
      301 acccctccca gggccttctg ttggatagat aggctttacc agtgcgctcg tcgtacatga
      361 gccagtggaa aagaacgcac gccggcagcc tgctcgcctt tatcacttcc atctggaacg
      421 acagtctttc acggtgcgac cagtcgtcgt cgtcccactg gcagaaatac tccccgctgc
      481 actccctgat agaacggttc ctgagttttc ccagattaaa ttcttccgat gaaggtatct
      541 cgaccagctt gagattacgg tcttccaggg tccttacgta ttcggccgtc tccccgtcgc
      601 cgctctcgta gagcagcacg agctctttgt tgggatacgt ctggtccctg aaggaccgca
      661 ccgccctacc gagtagattc gtcctcccgt gcgtgatgca taggcacgaa atgaggggtg
      721 attccgtcat gatggcttca ttattttccg acaagcgcgc tcaaaaacct ccgctttgtc
      781 ctatattctt cgttatcggg gcagcgctcg gaggcgagcg tgtagtattc gaggctctct
      841 ccgcgccttc cgagctggaa gaggcagtcg gcgaggggct cgtaggagag ttccctgaat
      901 atagcggtat tataggagag cgggccgtcc gtgagcatat cgggatcgat agagacgagc
      961 ttctctaaca cagaaacggc ttcctcgaaa cggcggctcc gggcgagcgt cacggccttg
     1021 gtccatataa ggagatagtt gtccgggaat agcgacaggg cctcatcgag gagccccgag
     1081 gggtctcccc ctttttcatt gacgagacag atcatctcgt agtaggcgtg gctgtcctcg
     1141 tgcgtgacgg cctctttcgc gcgcgagata tctatcgctt ccatccatgt tctttcagcc
     1201 ccttccatgt cgccgagccc tttttgcgcg acccccagct gccacctgag gtacatcctc
     1261 ccggggtccc gctctatctg tgagcgcagg agcggcaggt tcctctcgtg cttgtgagtg
     1321 agctcgccgt cgtagcccgt gtgatcgatc gcgagcgcgc tatcgcctat gccgagcccc
     1381 tcttgctcca tgaacttctc gatagacggg aaaattgtct cgtgtatcct gctgtaaaac
     1441 cgtatgcgcg ggtcgtttct gaatatgcgg tattccttgt aggccgtgta tccgcctatg
     1501 ggatggaacc tcaccgtgta tgcggcgttg ctcgtgtcgg agaggactcg cctcacctcc
     1561 tccctgctca cgggtctcag cctctcgtcg gcgtcgatgt agagtatcca gccgccccgg
     1621 caatgctcta gggcacggtt gcgcgcggcc gagaaattgc ccgtccacgg gaaatcgaac
     1681 accctcgccc cgtaccgggc ggcgatctcc ctggtagcgt cggtcgatcc cgtgtccact
     1741 atgactatct cgtcgacgac gtcctttatg gattcgaggc agccttcgag gaatttctcc
     1801 tcgttcctga caatcatgga ggctgaaaag ataatatcgc tcaattccgc ttcacctttg
     1861 cttaatgata accggagagg cgggagacat aaacggtctt aaactataat ttataaatac
     1921 aatagggact ccggacggcg cggatacgga gaacgccggg ataccctttt gcagccgggt
     1981 gagacactat agaatgatcg gatacgtctg tattcccctc aggcgataag tggcagttcc
     2041 tcttgccgtt cgggcctccg gacaatgaaa ccgaagcctg gcgaaagtgt gcttcccata
     2101 aggatgccgt cggcccagta ataacccgtg tcaccggaag ggagcaggtc gtacgtgaag
     2161 ccggcagagt aagggacctt ttcggcggat acgaccaccg cgccgtcgag gatatccccg
     2221 ggaacgatat cgccgatttt cctttcgtca gcagtaggat gccccgccga cacgcgcact
     2281 tccctgccgt cgtcgaggac caggcggcaa atcgtatgtg ttgaagggac tgacgtcttc
     2341 gatacgagct caagcggcat cgctgtcctg acgccgtccg catcgagcgt atacacgagc
     2401 attccgggtt tgagagcgaa gaccggtatg ttgccgagcg gagtggagat catagtgccg
     2461 aatgacaggc aaacggttcc gtcgttgctc tgtgcgttcg ccgtagaaag gttgagcttc
     2521 tggttgagca tcatcaccga aatgacggtc gggatgatga aagccccctt tgttactttt
     2581 tccaggaact ctcgcctctc ggggttaata ccgtccggaa attttttgtc gctcatcgta
     2641 tgccctcccg ttattttagt aacagattac gctcattata tcacggacaa tctttcctgt
     2701 aaaccggaga ccgctccctc gagcccgcct atatatatgg tatggcactc ggcgttgtct
     2761 atcatcctgc acaagtggct gatcttcatg tggtcgtcct tctccgagag gaagctcatc
     2821 ggcatgaccc tgtttaccat catgtacttc gggcactcct cgatacgggt ctccccgccg
     2881 tggttgggca cgagatagaa tacggcgcgc acctcgccct cgtcgatctt ccattcgcgg
     2941 ccgacgtcgt gaggagagag ggagtacatc ctgtggttat agtatgtcat gttgtaaggc
     3001 agcgtttcga agagcaattt caactgcgta aggaggtgcg tgctctcctc ctttatgttg
     3061 aaccgcctcg ggaacggcat ggccttcccg tctttgacga gcacgagctc gtcccccacc
     3121 acgcggaaac ccgccagcag cagcgtgatc atgagagtgg ttttcccggc gcccttgtcc
     3181 ccgacgacga ggaacctctt cccctcccat tccgcgcagc cggcgtgtat gcggatacgg
     3241 tccgttatac cggcgagagc ctccctgtgc atgagccact ggaggttgaa tatcgttttg
     3301 gagaggtcgt ccgaggaatg tacggcttcg tcgtttttca gtatcttaca ggcgccgccg
     3361 ctcacgttca ggtcgtacac ggactccttc gtgacgggat agtcctgctc ggcctcgtgc
     3421 actatgaaat cgagtaggcg cgccgattcc tcgttattgg tacgtattgt gaacgcggtg
     3481 tcgaggagtg tatatctacg ttcgtaagtt atttaaccag cccttcctgt ataagtgtgt
     3541 cgaggcagtc ggcgacttcc ttttccgggg ctgcatcgag cccgtatgcg tctttcacta
     3601 tcgccgttat gtcggcggtc gagttcttgc ccgtgcatgc ctctagcacg agcacggccg
     3661 tcttgttgag gtagtgcacc ttgtcctttt cgctctggta gatgacgtac ccgtcctcga
     3721 cctcgtttat gtctatgcct tccgcaatct tgggatattc gctcatctta atacgccccg
     3781 ctttcggatt tatttcgata caggttagtt attatcccga atcgcggctt gcgtttcaag
     3841 accgggggat atcaggccga acaatacaaa aaaccccggg acacctgtac cgatgcccgg
     3901 ggcaattcgt aaactcgatt atttcttacc gtaattctac aaaactcagg actgggtcga
     3961 atcgctgccc gatcccgtcg gctgctcgga cgttgtgtcc ttgtcctttc cgccgcaggc
     4021 gagaacgttt gtcgtcagga gcacggctcc gccgagcgcg attcccatgg tgattgcaag
     4081 cgtgaataat atctttttca ttttgactta cctcctgctt ttttgtattt aaagtttagc
     4141 ccagggattg aaatattgcc agttttttta ttttcccgct ggaatgtccg cacatttccg
     4201 tagtattttt ttgaatgatt cagggcttcc tcgtccacgc gcttatccat gtgttcgaca
     4261 tccccaccca gtcgagagca tcgctccccg ccgcttctgc gatgatcctt tccctgagcg
     4321 tgtcgatgcc cacttcttcg gcgctcgcga cgccgtgctt caccatcatc ggcacgaggc
     4381 tccttatcgt gctcacgaac cagtcgaggc ccgccgacgc cgccccgccc acgaacgcgt
     4441 ccatgtgcat tacgggctcg ggcagcccgg cagcgcggaa tacgggaagg agcttgaagc
     4501 ccatctgcat ctccgtcccc gctttctcca tcgccgcgta tagccagccc cagcacttca
     4561 tgaagagcgg gtaagcgggg atcgacacgg ggcggaccgt gaagtccacc tcctgaaata
     4621 cgactatgcc gcccggcttc acgtgccgga cgagcgcttt cagcacctcg accggccggg
     4681 gcacgtacat gagcaccgcg cggcccgcga gcgcgtcgaa tacgccctcg ggctcggccg
     4741 cgctgatgtc gccgtttacg aaccttatgt ttccgtaccc catctgcgcc gcgcgtttct
     4801 tcgccgtctc gagtatctcc gggttcctct cgatgctcac gacctcccct tcgtgcccca
     4861 ccatttgggc gagaaggagc gccacgtctc ccgcccctgt gccgagctcg agcaccctca
     4921 ttcccctgac tattccggct agctcgaata tcctccgtgt cgtctctatg ataatgcggg
     4981 actgctgctc gagccgtttt ttctcgttct ctgtgtcgct taacgcgtac tctttcttca
     5041 attccgtttc tgccatgtac agcctcccgt aattagtata accgtacaag cggcttaaac
     5101 attacaacac gtaatataca gattcgaccg tttggcgcat ccggcttcct ccgtaggagg
     5161 agtccgtttc gtaaaacatg acccggaacc atatttcacc tgacggctct cctccggaaa
     5221 tgtgcccgga tctctaattt ttaatctaca cgcctagttg tcttgaaatt acagaagtgt
     5281 tataatcgtt ttaggaattg gatttgtata aaatccctat ctaaggagaa tggttgacaa
     5341 tgagaacatt aaagttcttt ttgctattgg cgattctgtc agtatcaaat catgcatttg
     5401 cacaaattcc cgaatgcccg tgcgacactc tggtgctgca aaacgggact accggcaacg
     5461 aaatcgttga gcttttatgc cccggcggcg agctagccga gaatgtctta tcagtcgtac
     5521 atccgaatgc agttgttatc ggggacgaga atgggggata tgcagtggaa agcatcggag
     5581 gggcaggctt cgcctgtttg atcgctgatg aaggcggagg tcttgatgga attcaaatta
     5641 cggaggaaca gtttacagac tgcagggaac gactgatcca gggatgcaac ttaagcttta
     5701 gtcttcctat accgacgctc agtgaatggg gcatgataac aatggccggg gttttaggga
     5761 ttatcgggct gatcctcgcc cggagaagga aagtagtcgc ataaagcctg aaaacaatat
     5821 aatcgaaaat taaaagggga gcccgggctc cccttttttg ttacctttcc gattagcgat
     5881 tccagcttgc aatgtgtttg aagaaattga cggactgctc gtgtagtccc cgtcaataca
     5941 aagaaaatgg ataaacccgt cagtttttca ccggaatttg cgtctttttc cgcccctccc
     6001 cgtctatatg gatagaaaat aagaaaggag gcccgacatg gctgagaaag aagtaaagaa
     6061 agaggattcc ggggatgagg aatgcaggtg cggggaaaat acgtgctgca aggtcgtgtt
     6121 cgaatgcacg tgcacaggat gcgaatgcgg gtaaattccg cagaaattac gccgcgggcg
     6181 catgctccgg atatggaata taatatgagg tatgcgtccg tggggcgtaa tatatataat
     6241 cccatccccg tggagctcgt tcgggtggtt tccaagcaga tcggggagac agagaattat
     6301 atggatacga atagtgccgc agacgcgggt cagatatggg aggaattttc gggaagggta
     6361 aggtctcatc tcctcaaaaa aatccgcaac aagggagacg tggacgacct gatgcaggat
     6421 atattcatga agatacacgc ccacttaccg cacctgcgtg aacgcgacaa actctcatcg
     6481 tggatataca ggatcgccgg gaacacgctc aacgattatt acaggaaggg cccgggccgt
     6541 gaaacggagt ttgacgagaa caagaccgca gacgggaacg cggacgggga cgaccctctc
     6601 tccggtatag aagggtgtct cgaagcgttc atcgcgaggc tgcccgaaaa atacagggag
     6661 ccgctcgtga tgagcgacgt ggagggaaag agccagaagg agatcgctga agaaatggac
     6721 ctctcgtatt cggggctcaa gtccagggtg cagagggggc gggagatgat aaaagacatg
     6781 ttcatcgagt gctgcaagct ttcggtcggg aaggacggga agctgaaggg cgagctgggg
     6841 aacatggacg actgcacgct ctgcgggagg ggttaaaaca cccccctatg ccgtaggagc
     6901 ggcatcctac cgcgattcca catagttccc tctccattga ggggagaggg caagccctga
     6961 acttgtttca gggtgagggt gtaacatttc actaatctat tcaatcaaaa ccgttcgtcc
     7021 tggttgtccg gcgcagcccc tgacgaagcc gggagcctgt cgaaggacgg aaggacgaga
     7081 gttacgatcc tccgacagtt gtataacagg ccgcaattcg attataatca gttatatggc
     7141 cggaatcccg ttattcctca aatcaatcaa aagcaataat ttcctcggaa ttatattcat
     7201 cgcagccctt ctattttccg cctgcgacga gggaggcggg ggcgactgcg tgcgcataca
     7261 ggacgacccc gatttccttc aggatttcgt cgaatgccct acagacggcc ttcagatagt
     7321 ctgcaacagg ttcgactgcg tgctgtcgtt cccgatcgac gaccccgttc ctccgccgga
     7381 acagcttgta agaattaatc cccggaaatg cgaggcgata gactgcttca atatggaatg
     7441 cgggatgaaa aatcccttta caggtgaaga cctcgggagc gccgtctttt cgatagaaga
     7501 attccccggc gacggcacct tcgcaggcac cgcaggcaat caggatttca cctgctcgcc
     7561 ggttgttgaa tgagtcagac acgagaccgg tatgatatcc gtaggagcgg tatctcgccg
     7621 cgacttcgtt accccccttt cataaataag tgaagcattt ccgttcttcc cagcgccccg
     7681 cctccctgat gcggacagta tttacacttc cacatgcata tcgtattatt gtcttgagaa
     7741 gcgttttcgg aaaaggcaaa gatgagcggc aacagaaaat tgaggaagag cctcaggctc
     7801 gacttcagat acgagaactt caatatcctg ctcgaatccc agacccgcgt cgaaatcctt
     7861 cctcccccgt cggatcagtt agaagagatg gacgaatata acggttatta cgtcaaacta
     7921 accgacctgg agaataacgt tttctacttt caaaaactcc agaatccctt acgcacgcga
     7981 acagaatctt acggcgacga cgcggaaagc cagttcacgt acgaaaccct caaggaccct
     8041 gatccgacct tctcgatcct tatcccggac ccggggatcg ttttcacgct cgatatcata
     8101 aacaatcctc ctcagccgga gctatttttt aaaattgcga ggcaatcgtt cactttcacg
     8161 atcgacccgg cggaaataat acttcctcct atgcccgtcg ccgaaagagt ggag
//
LOCUS       DASSJH010000116         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_565237, whole genome shotgun sequence.
ACCESSION   DASSJH010000116 DASSJH010000000
VERSION     DASSJH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565237"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..848
                     /locus_tag="VFJ67_02505"
     CDS             165..848
                     /locus_tag="VFJ67_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET7289284.1"
                     /translation="MSESAKPGFATLMASSTQYKMFNFMVSILLLLVLSAVLEGTKYG
                     FLVVNTMSSIVFLLGTYAVGGNRRTLIILIILGLPWFLSEWAFTKSSRTIFTSMLFFI
                     FVTVTILDHILKSDEVTTDTLYGAVCVYLLLGIMWASIYGFLEYVSPGAVFKGYDKDI
                     NTKMSTNELIYYSYTTLATLGYGDITSVTPVGRILSVLEAIFGQLYIAFLVARLISIY
                     TTNALRKKG"
     gene            complement(863..1456)
                     /locus_tag="VFJ67_02510"
     CDS             complement(863..1456)
                     /locus_tag="VFJ67_02510"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET7289285.1"
                     /translation="MFRLRIEGIPKSLKLAVAVTFVMVLASSGVSVLFGERLLDFGEN
                     ILMSYGQANTDFILYIVTAVSSSPLTLPVSAYAVIGTLLGYEPGKLITLIALGAVTGS
                     SVSYLLGRCFGDTPFVRRRFPEMENHSWTRGRSLRVVSLILLGGAMSPVPVHPMYAAC
                     GMMRYPAALFVLIVFLGWWVRIGVVVMGIKFISDLTL"
     gene            1608..2630
                     /locus_tag="VFJ67_02515"
     CDS             1608..2630
                     /locus_tag="VFJ67_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HET7289286.1"
                     /translation="MSKRKIVIIPGDGIGPEITAAVIKVIEATGAEITWVEHEAGLAA
                     LDKGKDVLPDETLEAIKEHKVALKGPCTTPIGEGFTSVNVKLRKTLDLYAAVRPVRNL
                     PGVITRFRNVDIVIIRENTEGLYSGVENVVSPGVVMSMKVATEKACSRISHWAFEYAR
                     HRHRKKITVFHKANIMKLTDGLFIRKSKEASKEYGDIQYEEMIIDAGCMKLVQDPALF
                     DMLLLENLYGDLISDLCAGLVGGLGVVPGANIGDSEAVFEAVHGSAPDIAGKGVANPL
                     ALLMSGVMMLNHLADTEGREDFRACAEKIKHAYDGALQEGDRTRDLGGKLGTMEFAEA
                     LIKRLK"
     gene            complement(2681..>3485)
                     /locus_tag="VFJ67_02520"
     CDS             complement(2681..>3485)
                     /locus_tag="VFJ67_02520"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET7289287.1"
                     /translation="TVFVHPKHKPGNTVGSALGQLDTYDRIYKEHRDEIFQIKTKKDL
                     DMINGKGMIGFLTLMEGADPVEYPGKLGEFYERGVRMIGLTWNDRNQYASGNETAEGL
                     TDAGRDLIKKMNELRITLDLSHINENGFREAVELTTLIPVASHSNARALTDHPRNLRD
                     DQLLAISERGGVIGIVLYNQFLKTSDKTPTVEDVFKHTDYIVGLCGEDHVGIGSDMDG
                     ARIEDFPEDLKKISDLPKIAEYFLEKGYTEERVRKIMGGNFLRVIRENI"
BASE COUNT          784 a          943 c          920 g          838 t
ORIGIN      
        1 ccgaagctta atgcgtctac aatcaagagt gtctttacct ggcaattccc tgaacctgca
       61 ctgcgatccc ggcctgtttc tgtattatcc cctaccggtt ctttccgaaa tctactatta
      121 tttgagtatg attcattcct ctaataccgc cggaggtttt aagaatgtct gagagcgcaa
      181 aaccgggctt cgcaacttta atggcaagct cgactcaata caagatgttc aacttcatgg
      241 tctccatact gctcctgctc gtcctctccg cggtgctaga aggcaccaaa tacggatttc
      301 ttgtggtcaa tactatgagc agcattgttt tccttctcgg gacctacgcc gtcggcggga
      361 acaggcgcac actcatcatt cttataatac tggggcttcc gtggtttctc tccgagtggg
      421 cttttacgaa atcgagcagg accatattca cgagcatgtt atttttcatt ttcgtgaccg
      481 tgactatact cgatcacata ctgaaatcgg atgaggtgac gaccgatacc ctctacggcg
      541 ccgtctgtgt ttatctcctt ctcggtatta tgtgggcgtc gatatacggc tttctcgaat
      601 acgtatcgcc gggggccgtc ttcaaagggt atgacaaaga tatcaatacg aagatgagca
      661 cgaacgagct catatattac agctatacga cgctcgccac tctcggatac ggcgacatta
      721 catccgtcac gcctgtcggg aggatactct cagttctcga ggcgatattc gggcagcttt
      781 acatcgcgtt cctcgtggcc aggctgataa gcatctatac gacgaacgcc ttaagaaaga
      841 aaggctgagc cgcccatccg tttcataacg tcaaatcgct gatgaattta atccccatga
      901 ccacgactcc tatcctgacc caccagccga ggaacactat cagcacgaat agcgccgcag
      961 ggtagcgcat catcccgcac gcggcgtaca tagggtggac gggtacgggc gacatcgcgc
     1021 ccccgagcag gatcaggctc acgaccctga gcgagcgccc cctggtccag ctatggttct
     1081 ccatctcggg gaacctcctc ctcacgaacg gggtgtcgcc gaaacacctt ccaagcaggt
     1141 acgacaccga tgagcctgtt acagcgccga gagcgataag ggttatcagc ttccccggct
     1201 catatcccag cagcgtcccg atgactgcgt atgccgaaac cggcagcgta agaggagagc
     1261 tgcttactgc cgtgacgatg tacagtatga agtccgtatt cgcctgcccg tagctcatga
     1321 ggatgttctc gccgaaatcg aggaggcgct ccccgaacag aaccgagacg ccggagctcg
     1381 caagcaccat tacgaaagta acggccacag ccagctttaa agacttcggg atcccctcaa
     1441 tccttaacct gaacatcgct catcatcagt gaaacacggc agtctttggt ttcggttaag
     1501 ccggggtgaa tacggagtga agttaaaaat gcacgagggg gataatttta cggattttta
     1561 tcgcctgtaa ttgctaaccg cgataaatca gggttcaatt atcttccatg agtaaacgta
     1621 aaattgtgat aattccgggg gacggaatcg gccccgagat aacggcggcg gtgattaagg
     1681 ttatagaggc gacaggggcg gaaatcacgt gggtcgagca tgaggccggt ctcgctgcgc
     1741 ttgacaaagg taaggacgtg cttcccgacg agaccctcga agccataaaa gagcacaagg
     1801 tcgcgcttaa gggtccgtgt accactccta taggcgaggg ttttacatcc gtaaacgtga
     1861 agcttagaaa gacgctcgac ctctatgctg cggtaaggcc tgtgaggaac cttcccggag
     1921 ttataacgcg cttcaggaac gttgacatcg tgataatcag ggagaatacg gaggggcttt
     1981 acagcggggt cgagaatgtg gtttcgccgg gcgtcgtgat gagcatgaaa gtcgcgaccg
     2041 agaaggcgtg cagccgcatc tcgcactggg cgttcgaata cgcgcgccac cgccacagaa
     2101 aaaagatcac ggtgttccat aaagccaata tcatgaaact gacggacggt ctctttatca
     2161 gaaaatcgaa ggaggcgagc aaggagtacg gcgatattca gtatgaggag atgattatcg
     2221 atgccggctg catgaagctc gtacaggacc cggccctatt cgatatgctc ctgctcgaga
     2281 acctctacgg cgacctcata agcgacctct gcgcgggcct ggtcggcggt ctcggagtcg
     2341 tgcccggggc caatataggc gatagcgaag ccgtattcga ggctgttcac ggctctgccc
     2401 ccgatatagc cggaaagggc gtggcgaatc ctctggcgct tctcatgtcg ggcgtcatga
     2461 tgctgaacca ccttgcggat acggagggac gggaggattt ccgcgcctgc gcggagaaga
     2521 taaaacacgc atacgacggc gctcttcagg agggcgacag gacgcgcgat ctcggaggca
     2581 agctcggcac tatggaattc gccgaggcgc tgattaagag attaaagtaa gctgtcgtgt
     2641 aacctggaca tgcttatgga cgcttggtct ggattgtgtg ttaaatgttc tccctgatca
     2701 ccctcaggaa gtttccgccc ataatctttc taaccctttc ctctgtgtac cctttttcaa
     2761 ggaagtattc cgctatcttc ggaaggtcgg atatcttttt caggtcttcg ggaaaatcct
     2821 cgatcctcgc gccgtccatg tcgctcccaa tgccgacgtg gtcttcaccg catagcccta
     2881 cgatatagtc cgtgtgtttg aatacgtcct cgaccgtagg cgttttatcg ctcgtcttca
     2941 ggaactggtt atagagcacg atgcctatca cgccccctct ttccgatatc gcgaggagct
     3001 ggtcgtccct gaggttcctc gggtggtcgg tgagggccct cgcgttcgag tgtgacgcga
     3061 ccggaatgag cgtcgtcagc tctaccgctt ccctgaaacc gttttcgttt atgtgagaga
     3121 ggtcgagcgt gatgcggagc tcgttcattt tctttatgag atcccttccg gcatcggtaa
     3181 gtccctcagc ggtctcgttc cccgaggcgt actggttcct gtcgttccac gtgagaccta
     3241 tcatcctcac tccccgctcg tagaattcgc caagctttcc gggatattcc accgggtcag
     3301 cgccttccat aagcgtgagg aatccgatca ttccctttcc gtttatcatg tccaggtctt
     3361 ttttcgtttt tatctggaat atttcgtccc ggtgctcttt ataaatcctg tcgtacgtgt
     3421 cgagctgccc gagcgccgat ccgaccgtat tccccggctt gtgcttcggg tgtacgaaaa
     3481 cggtg
//
LOCUS       DASSJH010000117         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1215123, whole genome shotgun sequence.
ACCESSION   DASSJH010000117 DASSJH010000000
VERSION     DASSJH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215123"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFJ67_02525"
     CDS             <1..534
                     /locus_tag="VFJ67_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7289288.1"
                     /translation="MAIVTSMMSGPAITLILRLGKKRTLQDALSSRLFTRELQAFSSR
                     MAIHELASMACEASGLNTSEVEAAVWEREETLPTGIGNGVALPHARIDGISKPILAVG
                     ISDTGIDFDAPDDKLAHIVFLILTPASDPEAQLEITAGLARLFREHGMTERVLKTKSY
                     TEFLALVKSLPVEAEAA"
     gene            complement(583..1470)
                     /locus_tag="VFJ67_02530"
     CDS             complement(583..1470)
                     /locus_tag="VFJ67_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289289.1"
                     /translation="MKATEGNGRGWMRAIPSEIAGMSAFDLALRLTLLDLLLRPVGDW
                     TVRPLTVAFAAVGLLIPGQFRSNFIWMPLAFLTGLRVFLDWPLPDNHAYLLFYWCLAV
                     SIATVSRDAGRCLSFNGRMMIGLAFAFSVLWKVFLSGDFMDGRFFRIAMLTDHRFEWF
                     TQMVGGLTAVEYENLKQFVSQHVDGHFPFSGREIPPQPARFVMVSQLLTYWTVFIESA
                     VALLFLVPGERGLLRFRDFFLVLFCVTTYSVATVEGFGWLLVAMGVSQSGDGRKAVRF
                     LYIGAFLLIIFYRFAAKIW"
     gene            complement(1457..1879)
                     /locus_tag="VFJ67_02535"
     CDS             complement(1457..1879)
                     /locus_tag="VFJ67_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289290.1"
                     /translation="MGIKSRICYFPVVLLLAVSVLQLYLAHNYALAPWSGGGFGMFST
                     PDAAQSRHIHAYAVSPGVRREIVIPPSLRDRALRTAALPTESNLKKLARELSDIPTPE
                     NEAVGSIVIQVWRTNYDPVTLMPSGSMIRSLEVEIESH"
     gene            complement(1960..>2143)
                     /locus_tag="VFJ67_02540"
     CDS             complement(1960..>2143)
                     /locus_tag="VFJ67_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289291.1"
                     /translation="ATAIAVGTALTVSAFNSLTCTPTIVSVGGVSYYQCGSNWYNQAY
                     QGGDVVYVSVGAPPGY"
BASE COUNT          541 a          623 c          578 g          401 t
ORIGIN      
        1 atggctatcg ttacttcaat gatgagcggc ccggcgatta cacttatact ccggctcggt
       61 aaaaaacgaa ctctccagga cgcgctgtca tcaagacttt ttacgcgcga gctgcaggcc
      121 ttctcaagcc gcatggctat acacgagctt gcgtcgatgg catgtgaagc gtccggactc
      181 aacacgagcg aggtggaagc ggcggtttgg gaaagggagg agactcttcc cacggggatc
      241 ggcaacggcg tcgcgctgcc ccacgcccgt atcgacggca tcagcaagcc catattggcg
      301 gtcggcatct cggatacagg aatcgatttc gatgcgcccg acgacaagct ggcccacata
      361 gtttttctca tcctcacgcc ggcgagcgat cccgaagcgc agctcgaaat caccgccggg
      421 ctcgcgaggc tcttccgtga gcacggcatg acggagcggg tcctaaaaac caagagctat
      481 accgaatttc tggcgcttgt gaagagtctg cccgtcgagg ccgaggccgc ctagcttatt
      541 tacagaaggc gcagccttgt tccgaacgat cgtcctgtgt ttctaccaaa tctttgccgc
      601 gaacctgtaa aagataatga ggagaaaggc cccgatataa aggaacctca cggctttcct
      661 tccatctccg ctctgagaca cacccatagc tacgagaagc cagccgaacc cctccacggt
      721 cgcgacagaa tatgtcgtca cacagaaaag cacgaggaaa aagtcacgga aacgcaaaag
      781 tcccctctcc cccggtacga gaaaaagcag cgccacggcg ctctcgataa ataccgtcca
      841 gtaagtgaga agttgtgaaa ccattacaaa acgggccggc tggggcggta tttccctccc
      901 ggagaacggg aagtgcccgt ccacgtgctg actcacgaac tgcttaagat tttcgtattc
      961 cacggctgta agcccgccga ccatttgcgt gaaccactcg aaacggtggt ccgtcagcat
     1021 cgcgattctg aagaaccgtc cgtccataaa atccccggac aggaacacct tccacaaaac
     1081 agagaacgca aaggcgagac cgatcatcat tctgccgtta aacgagagac accgtccggc
     1141 atctctagat acggtcgcga ttgagactgc gaggcaccag tagaaaagca ggtatgcgtg
     1201 gttatcggga agcggccagt ccagaaaaac gcgcaagccg gtaaggaaag caagaggcat
     1261 ccaaatgaag ttgctcctga attgacccgg gatcagaagt ccgacggccg caaaggcgac
     1321 agtcaaaggt ctcaccgtcc agtcccctac cggtcgcagc agaaggtcga ggagcgtgag
     1381 cctgagggcg aggtcaaaag cgctcatccc ggcgatctcg gaaggaatag ccctcatcca
     1441 gccgcggcca ttaccttcag tggctttcaa tctcgacctc cagagacctg atcatggaac
     1501 ccgaaggcat gagtgtcacg ggatcgtaat tggtcctcca gacctgtata actatggaac
     1561 cgactgcttc attctcgggt gtggggatat ccgacagctc gcgtgcgagt tttttaagat
     1621 tggactcagt tgggagagcg gccgtcctca atgccctgtc gcgcagggac ggaggtatta
     1681 cgatctccct cctcacgccg ggactcaccg cgtaggcgtg tatgtgtctg ctctgagccg
     1741 cgtccggggt cgaaaacata ccgaaccccc cgcctgacca gggggcgagt gcgtaattat
     1801 gcgccagata taattgtagg accgacaccg cgagaaggag aacgacaggg aaatagcaga
     1861 tgcggctctt aatacccatg aaattcatca ccgatccgcg ccgttccgca tttcaacccc
     1921 tggtggttac gccgaactga tacattgaca gagcgaatat cagtagcccg gaggagcccc
     1981 aacggataca tacaccacgt caccaccctg atacgcctgg ttataccagt tcgagccgca
     2041 ctgataataa ctcactccgc ctacagacac tatcgtgggg gtgcaggtga gcgaattgaa
     2101 cgccgacacc gttaaagctg tgccgaccgc aatagcagtc gcc
//
LOCUS       DASSJH010000118        10750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1106834, whole genome shotgun sequence.
ACCESSION   DASSJH010000118 DASSJH010000000
VERSION     DASSJH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10750
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106834"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFJ67_02545"
     CDS             <1..387
                     /locus_tag="VFJ67_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7289292.1"
                     /translation="IVDADFGWQQDVVFDELGNDDNVSWWGIAGYVVVDPHPAVRLAL
                     RGEYFDDPEGYRTGLDQKLFEFTPTLILKPFKGLIAGNKFLDNFEARAEFRWDHSDEN
                     FFITKDEGLKKDQYGVMGQLLYWIQL"
     gene            613..685
                     /locus_tag="VFJ67_02550"
     tRNA            613..685
                     /locus_tag="VFJ67_02550"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:646..648,aa:Phe,seq:gaa)
     gene            909..1574
                     /locus_tag="VFJ67_02555"
     CDS             909..1574
                     /locus_tag="VFJ67_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET7289293.1"
                     /translation="MISRTIEDYLKAIYKLRKHRERVTTSAIAQKLGVTDPTVTAILK
                     KLANERYLKHISYQGVVLTDKGEKIAIGVIRKHRLVELFLNEILGLDWDKVHVEAEKL
                     EHVISDDVLARMDHVLGYPKIDPHGDPIPTGEGRIENAEINQSLADTEVGEPVAISRV
                     SDDDPDKLRFMAKLGLFPNVKLRITDKSPFDGDISIKVGSSHHRLPRDVAKSIYVTQA
                     GKV"
     gene            1599..3509
                     /locus_tag="VFJ67_02560"
     CDS             1599..3509
                     /locus_tag="VFJ67_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7289294.1"
                     /translation="MASFRSGSNNQNSLAEVYESVNVPSLGSWWRRWLAFSGPALLVS
                     IGYMDPGNWATDLAGGSRYNYQLIWVLLMSNLMAILLQSLSARLGIVARRDLAQANRE
                     EYPGLINIPLYLLAEIAIIACDLAEVLGSAIALNLLFGLPVIYGVLITALDVLVLLML
                     SRFGIRKLEALILSLVTTISVCLLIEIILAKPEWGGIVRGFAPSLPNKDALYIAIGIL
                     GATVMPHNLYLHSSLVQSRKIGDDRTSIKQAIRWNTIDSALALNIAFFVNAAILVMAA
                     AVFYKSGHYEVAEIQDAHRLLEPLLGASIAPIAFAVALLASGQSSTITGTFAGQIIME
                     GYLNLRIRPWLRRLITRLLAVVPAALTVLYFGENSTGSLLVLSQVLLSLQLPFAIIPL
                     IHFVSDKRRMGDFSSPRWLNLIAWLAAAIIVSLNVKLVVDEIQSWLTGAGSRAWTIEL
                     TVLPLTISIGALLIYITIRPWIRKLRMPWVGVHGPAAASDAELPTIEPYRRVGVAIDF
                     SNGSLRLLAEAQRMARSGGAKIFLLHVVESPAAQVMGQEAADQEMLSDKGRLEELTRK
                     LRNMGFDAEWRLGTGDPASELARIINNLNIDLVILGGHGHSGISDIIHGTTVDSLRHQ
                     VHASILVVPLKA"
     gene            3730..4143
                     /locus_tag="VFJ67_02565"
     CDS             3730..4143
                     /locus_tag="VFJ67_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7289295.1"
                     /translation="MRFGDYLKSKRKQRNITQEDLARSLNVSSVFIHQLETGKVDAPS
                     IDRCNQIAAILEVNIEELWTVAKKERLKRFMEKEEITEDSFEVLTDAEKALINLYRSL
                     DSDMRRDFGGMIFMLLRHSQDEGVQEILEEFIKCA"
     gene            4158..4898
                     /locus_tag="VFJ67_02570"
     CDS             4158..4898
                     /locus_tag="VFJ67_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289296.1"
                     /translation="MILKALGIELCILALIGIVFLFQFNKAKGEFVNKTRAISETIGD
                     LASDFYSEQGKQPTSGEFYHFLDKRLGRKKLFNTFEIAPKFFSVVFKKDVENSLASDG
                     LFMKEFYPEGGYTVKDNNGIISVSVPFSTQTETEPYGIVKIDSDTKAILKKVLADNYL
                     LYAAMLIVLNNQAFIFYLLMRKKKEIVFEKGYLKEHSIGALKIMHKVLGDIIDDHRTD
                     YEGPDQAKRQESGKNVISISELAEKRKQ"
     gene            4895..5284
                     /locus_tag="VFJ67_02575"
     CDS             4895..5284
                     /locus_tag="VFJ67_02575"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HET7289297.1"
                     /translation="MKRLILAVFLVVFIAALGCDSPNGNGVVQLDGPILESMDADGNL
                     EFNGAVINTGDAAVKSVYVVIVLKDQDGNVIEANSVSIFDEDPNALLYPSERAFFSLS
                     VSSDPNRVFSKEVEVYYEDSPDNQPSS"
     gene            5741..6790
                     /gene="pilM"
                     /locus_tag="VFJ67_02580"
     CDS             5741..6790
                     /gene="pilM"
                     /locus_tag="VFJ67_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET7289298.1"
                     /translation="MFLSKKELVGLDIGSNAIKLVELKSSKNGYQLKNIGEALLPHYS
                     IVNKIIEKHDAVADTISSLFADLRVRTRNVAISISGHSVIIKKVSLPKMSDKELRESI
                     PWELEQYIPQSIQEVNYDYQVIPGETPEGNIDVLIVAAKKDVTNSYVSIVKDVGLNPV
                     VVDVDVFALENMYEINYPDSEGLVALVNIGASVTNINVLKDGVSIFTRDISTGGNQFT
                     DWMVNDLRIEYDEAERMKSTIREGEMPADLEPMVQNFNELICGEIKRTLDFFSSTLWT
                     SKVGNILIGGGSSKVQGLKDLLADLTDANVDFMNPFRRITYDKNEFDPEYIDAIAPKM
                     CVVMGLATRKPGDKS"
     gene            6787..7407
                     /locus_tag="VFJ67_02585"
     CDS             6787..7407
                     /locus_tag="VFJ67_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET7289299.1"
                     /translation="MIRINLIPEPERREVKGGGEIFAGICVLVALFVAIAALHIIQAK
                     KIDEVNRSIAKAEKRIKTLEAVKKKVDDFKAKNAELNRRIEIIGVLEKNRTGPLYVMD
                     ALSRAMPNKAWVDEFTEKGGQTKLVGIADNEFTVADFMQALQSSPYFVGVELGVIKKT
                     ELRKLDLRTFVINTRLDYTGGAGKQKKEQEPKAPGQDTGRKKAVTQ"
     gene            7407..8039
                     /gene="pilO"
                     /locus_tag="VFJ67_02590"
     CDS             7407..8039
                     /gene="pilO"
                     /locus_tag="VFJ67_02590"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET7289300.1"
                     /translation="MSFLDDYTERLSEQPLHVKAGILLLVVAVISAAYWYFFWSPKAE
                     VLEQAQKKLKQEEIKVKEYEAIAAELPKFEKEYQRLEKEFELVARKLPKDKEIPALID
                     GVYSDIAASNLDSIIFAPQAQVTKEIYAEIPIEMEVIGTYFNLADFFDRISRLPRIVN
                     VRDLQLTRNDIRGGTVILNAKFNVVTFRLLPPQEAKPQDTKKTKGKKRGK"
     gene            8063..8722
                     /locus_tag="VFJ67_02595"
     CDS             8063..8722
                     /locus_tag="VFJ67_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HET7289301.1"
                     /translation="MIKTKYGLFKLLVVLLLVLWSLQAGTAQEAEAPADDTLSQGLNV
                     IKDAKDNKLVTPEERSAEAVGEEAPSPEQSPAPGEGQGVPAAPEAGRGDALMSRDEKP
                     PYDSTGMRDPFKPFIKLADTPAAPSLVLRPPIQRYPLNQFRITGIVWIGGKPQAMVVD
                     PEGNTYFLGVGDKIGSSDGEILEVRQDGILVEEKTRLENVYGEVKFEVKQSVLAFQNE
                     E"
     gene            8768..10696
                     /gene="pilQ"
                     /locus_tag="VFJ67_02600"
     CDS             8768..10696
                     /gene="pilQ"
                     /locus_tag="VFJ67_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HET7289302.1"
                     /translation="MRILIISIALAACILYSGLEAHSSQAGQNYSSNYPPAAAQGADG
                     GESVATPTPAELAQVDAATVTVPQNTTANMPNPSDEYNHIESIGFEVQQGMGIVTVGT
                     SWNTQYERLQDEDKKITLKLKDVILADKFQVSRDVSDFDSPINFISSFRDPIKPNDVI
                     MVIELKEKDVPVDIGQNGNTITMNFGETREQEKIAAEKGEVEVAVKPEDLYAFDFRLT
                     SMPGAVKTYKGQLVSFDFKDADIRDVLRIISDISGLNMIISRNVSGTVTLKLTNVPWD
                     QALDVILEDAGLGAFIDGNVLRVAPLGTLQARQKAVKKAAETSEELEPLITKQVFVNY
                     ATAEELIPLVEPLLSDRGELRVDVRTNSILINDTGVRVAQIEGLVADLDTRTPQVLIE
                     SRIVQATLNFTRELGIQWGFDYRASAATGNPTGATFPSSVEVGGTTTGSPFGTTGDNF
                     IIDLPAAAGTGAGGVLGLVLGSLTGAYDLDLRLSALEARGDGRVLSSPKVLTLENTPA
                     RIEQGVSIPFLSVSAAGTQTQFVDATLSLEVTPQVTNDNRILMTIVVTDNRPDTALTG
                     AGGQPSIRRNEATTQVLASDGETIVIGGIFTRNVTDTDNSLPWFSRLPLLGWLFQRSA
                     TTDERRELIVFITPRIIR"
BASE COUNT         2790 a         2596 c         2954 g         2410 t
ORIGIN      
        1 atagtcgatg ctgacttcgg atggcagcag gacgtagtat ttgatgagct cgggaatgat
       61 gacaatgtct catggtgggg tatagcggga tatgttgttg tcgacccgca cccggctgtc
      121 agactcgcgc tcaggggtga atacttcgat gaccccgagg gctacaggac cggtcttgac
      181 cagaagctct tcgagtttac gccgaccctg attttaaaac ccttcaaggg tctaatcgcg
      241 ggcaacaaat tcctcgacaa cttcgaggcg cgggctgagt tcagatggga ccattcggac
      301 gagaatttct tcatcaccaa agacgagggt ctcaagaagg accagtacgg cgtgatgggg
      361 cagctgcttt attggattca actgtaattg atttgattta gtgtactcat ctctccatat
      421 ctcctttgtg tgggcggggg ggagaacagc tcgaagttat agctgttctc ccttttgtct
      481 attctgggct tccggtgcgg aaatatgaat aattttctcc cttgcctgta aatttacagt
      541 taggtaaaat taactccgct cttttgtatt tatgtattcc aggcccgctt actacttttt
      601 taaacaatat atgcccggat agctcagtcg gtagagcaga ggactgaaaa tcctcgtgtc
      661 ggcggttcaa ttccgtctcc gggcatgccc atttgtagta attatggtca catgcaattc
      721 atctcattcc gggatacccg tcctgtcata gaatcgtaaa agcacaatcc ataatatttt
      781 caaatatcca taagcagggg tccacagtta aggatcggga agaaagggat aaaaaaagtc
      841 cgcctgaaag aattcgcatc tccccgttga aatttagaca gtcctaatat attatttagg
      901 tgattcaaat gatcagccgc actatcgagg attatctaaa agccatatat aagctccgga
      961 agcaccggga gagggtgact acctccgcaa ttgctcagaa gcttggagta acagacccca
     1021 cggtaacggc tattttgaag aagctcgcca atgagaggta tctcaaacat atttcgtacc
     1081 agggcgtcgt tcttacggat aaaggcgaga agatagcaat aggagtcata cgcaagcacc
     1141 gtcttgtgga gctcttcctg aacgagattc tgggtcttga ttgggataag gttcacgtcg
     1201 aagctgaaaa gctagagcat gttatatcag atgacgtatt agcgaggatg gaccacgtcc
     1261 tcggctatcc gaaaatagat cctcacgggg acccgattcc aaccggagag ggcagaattg
     1321 aaaatgccga gataaatcaa tccctggccg atacggaggt cggcgagccc gttgcgatca
     1381 gccgcgttag cgacgacgat cccgacaagc tccgtttcat ggccaagctg ggtttatttc
     1441 caaatgtaaa gttacgcata acggataaat cccctttcga cggagatata tccataaagg
     1501 tgggctcgtc acatcaccgg ctgccgcggg atgtggcaaa gagcatatac gttactcaag
     1561 ctgggaaagt gtaaatcttt ggattaggag aaaatgcagt ggcgagtttt cgttccggct
     1621 caaataatca gaactcttta gcggaagtct acgaatctgt aaatgtgccg agcttgggct
     1681 catggtggcg gcgctggctt gctttcagcg gcccggctct gttggtctcg atcgggtaca
     1741 tggaccccgg gaactgggct accgacctgg caggcggaag ccggtacaac tatcagctga
     1801 tctgggtgct gctgatgtcc aatttgatgg cgattcttct ccaaagtctt tctgcccgcc
     1861 ttggtattgt tgcccgtcgc gatctggcgc aggcaaaccg tgaagagtat cctggtctca
     1921 tcaatattcc gctctatctc cttgcggaga tagccattat agcgtgtgac ctcgccgaag
     1981 tcctcggttc tgctatcgct ctcaatctgc tcttcggatt gccggtcatt tacggggtct
     2041 tgatcacggc cctcgatgtt ctggtgctcc tcatgctgtc ccggtttggc atacggaagc
     2101 tcgaagccct gatcttatct ctggtaacga ccataagcgt atgtcttctt atcgagataa
     2161 tcctggctaa gcccgagtgg ggagggatcg tgcgagggtt cgctccttcg ctgcctaata
     2221 aggatgccct ttatatcgct ataggtattc tcggtgcgac ggttatgccg cataacctgt
     2281 accttcactc ctcactggta cagtcacgga agataggaga cgaccgtacg agcataaagc
     2341 aggcgatccg ctggaacacc attgattccg ctcttgctct gaacatcgct ttcttcgtaa
     2401 acgccgcgat tcttgtgatg gcagcggctg tcttttataa aagcggtcac tatgaagtgg
     2461 cggagatcca ggacgcacac cgtttgctgg aacccctgct cggcgcgtca attgcgccta
     2521 tcgctttcgc cgtcgccctc cttgcctcgg gacaaagctc cacgattaca ggaacgttcg
     2581 ccggtcagat cataatggag gggtacctaa acctccggat tcgtccctgg ctcaggaggt
     2641 tgatcacacg gcttcttgcg gtcgtccctg cggcgcttac agtattgtat ttcggtgaaa
     2701 actccacggg ttccctcctt gtgctcagtc aggtgctgct gtcattacag cttcctttcg
     2761 ctataattcc tctcatccat ttcgtatccg ataagcgccg tatgggggat ttctcctctc
     2821 cccgctggct taatttaatc gcatggttgg cggcggccat tatcgtttct ctcaacgtca
     2881 aactggtcgt agatgaaatc cagagctggt taacaggtgc cggcagccgc gcctggacaa
     2941 tagagctcac tgtccttcct ctaaccattt cgatcggcgc actccttata tatataacta
     3001 tccgtccctg gatacgtaag ctcaggatgc cctgggtcgg ggtccatggt cccgccgccg
     3061 cttccgatgc cgagcttccg acgatagagc cctacaggcg cgtgggggtg gctatagatt
     3121 tctcaaacgg aagtctgaga ctccttgccg aagcgcagcg catggcacgc agcggcggcg
     3181 caaagatatt tttattacac gtggtggaaa gcccggctgc gcaggttatg gggcaggagg
     3241 cagccgacca ggagatgctg agtgataagg gacggctcga ggagcttaca cggaaactgc
     3301 gcaatatggg atttgatgcg gaatggcgtt tagggacagg ggaccccgct tcggagctgg
     3361 cgcgtattat caataacctc aatatcgacc tcgtgatact cggcggtcac ggtcattccg
     3421 ggatatccga cattatccac gggacgacgg tggacagtct ccgccatcag gtccatgcga
     3481 gtatactggt tgtaccgtta aaggcgtaac ataagcgcct ggttcatgaa tgaacccctt
     3541 aaaacagctc ctaaatcgca tattacacgc ctgccaccgg catatccggt gagtgaatct
     3601 aaaaaacgca aagaatttat ccatttaggc gattattagt cttgctattc catcaagtta
     3661 tgatgtaaaa ttaataaggc gagatttgtc caaataaaac aggaatcctt taaccttttg
     3721 gaggcaataa tgagatttgg cgactacttg aagagcaaga ggaagcaaag aaatattacc
     3781 caggaggacc tggcccgttc ccttaacgtg tcgagcgtgt tcattcacca gctcgaaacg
     3841 ggcaaggtgg acgcgccgtc gattgaccgg tgcaaccaga tagcggcaat ccttgaggta
     3901 aatattgaag aattatggac ggttgcgaag aaagagcgcc tcaagaggtt tatggaaaag
     3961 gaagagatta ccgaagacag cttcgaagtc ctgacagatg ccgagaaggc cctcattaac
     4021 ctctacagga gcctcgattc cgatatgaga agggatttcg gaggaatgat attcatgctt
     4081 ctgagacatt cgcaggacga aggagtgcag gagatactcg aagaatttat caagtgcgca
     4141 tgatgagtaa agtttatatg atcctcaagg cattaggaat agagctttgc atcctggcat
     4201 tgatcgggat cgtattcctg ttccagttca ataaggccaa gggggaattt gtaaataaga
     4261 ccagggcgat ctccgagaca atcggcgacc tggccagcga tttttattcg gagcaaggca
     4321 aacagcccac gagcggcgaa ttctatcatt ttctcgacaa gaggctggga aggaagaagc
     4381 ttttcaatac cttcgagata gcccccaagt ttttcagcgt cgtattcaag aaggacgtgg
     4441 aaaacagcct cgcgtcggac ggtctgttca tgaaggagtt ttatcccgag ggcggctaca
     4501 cggtaaagga caataacgga ataatctcgg tttcggtccc cttcagcacg cagacggaga
     4561 ccgagccgta cggtatcgtt aaaatcgatt ccgatacgaa ggcgatattg aaaaaggtgc
     4621 tggccgacaa ctaccttctc tacgcggcca tgcttatcgt gctaaataac caggctttca
     4681 tcttttacct cctgatgagg aagaagaagg agatcgtctt cgagaagggt taccttaagg
     4741 agcattcgat cggggcgctt aaaattatgc ataaggttct gggcgacata atcgacgatc
     4801 ataggactga ttacgagggt ccggatcagg ccaagagaca ggaatcggga aagaacgtga
     4861 tatcgatatc cgagctggcg gaaaagagaa agcaatgaag agactcatcc tggccgtttt
     4921 tctggtcgtt tttatagccg ccctgggctg tgacagccct aacggaaacg gcgtagtcca
     4981 gctcgacggg cctatactcg agagcatgga tgccgacggc aacctcgagt tcaacggtgc
     5041 agtgataaac accggcgatg ccgccgtcaa gtctgtttac gtggttatcg tattaaagga
     5101 ccaggacggc aatgtgattg aagccaactc tgtgtctata ttcgacgagg atccgaacgc
     5161 cctgctgtat ccgtccgaaa gggctttctt cagcctctcg gtaagctccg atccgaacag
     5221 ggtcttctcg aaggaagtcg aagtctatta tgaagattcc ccggataacc agccgtcttc
     5281 ctgaaacatt ttttctcaca cctcagatac tgcattaggg tctattctga tttcatctca
     5341 aatagtgata atatgcgggt agttaagccg tctaattgaa accggtatat tagtttgcga
     5401 tggacgatgc tcgattatcc ggaaacccgc cggcattcgg gttctatgaa cctcccaagc
     5461 cttccctgag gatcggtaat tcacttccac agccgtgacg gcctttcgtt tccatgcatg
     5521 cgaaacatcg ttaagatgct gtcattacag gatattagtc tgaattctgg ggcctctgcc
     5581 cttctcaata tccgtgtact gtccatctcc ttattgttaa aatcaaggtt tacaatagtc
     5641 tattgacaac tattaaagag cagtttgaca ttttttaaac tgagtattgc aataacttca
     5701 aaaaggtggt taaattagta ttgtaacata ggaggatgat ttgtttttaa gcaagaaaga
     5761 gctggtaggg ctcgatatag gttccaacgc tataaagctc gtagagctga agtcgtctaa
     5821 aaacggctat cagctgaaga acatcggtga agccttacta ccccattatt ccatcgtcaa
     5881 taagatcatt gaaaagcacg acgccgtggc tgacacgatc tcctcattgt ttgcagacct
     5941 tagagtcagg acgaggaatg tggcgatttc gatatcgggc cattcggtga taataaaaaa
     6001 ggtaagcctg ccgaagatgt cggataaaga gctcagggaa tcgatcccgt gggagctcga
     6061 gcagtacata ccccagagca tacaggaagt taactacgac tatcaggtca tcccgggcga
     6121 gaccccggag ggcaacatcg acgtgcttat agtcgcggcc aagaaggacg tcacaaactc
     6181 ctatgtctcc atagttaagg acgtcggcct gaatccggtc gtcgtggatg tcgacgtgtt
     6241 cgcgctcgaa aacatgtacg aaataaacta tccggattcg gagggattgg tagcgctggt
     6301 caacatcggc gcatcggtga ccaatattaa cgtacttaaa gacggcgtct cgatattcac
     6361 gagggatata tccaccggcg gcaaccagtt tacagattgg atggtaaacg acctccggat
     6421 cgaatacgac gaagcggaga ggatgaagtc gacgatacgc gaaggggaaa tgcctgcgga
     6481 tctcgagccc atggtccaaa atttcaacga gctgatatgc ggggagatca agcggaccct
     6541 cgatttcttc tcctcgaccc tatggacaag caaggtcgga aatattctca tcggcggcgg
     6601 ttcgtccaaa gtgcagggtc tgaaagacct gctcgcggac ctgaccgacg cgaacgtcga
     6661 ttttatgaat ccgttcaggc gtatcacata cgataaaaac gaattcgacc ccgaatatat
     6721 agatgcgata gcgccgaaga tgtgcgtggt aatgggactc gccaccagaa agccggggga
     6781 taagtcatga tccggataaa ccttatcccg gagccggaga ggagggaagt caagggagga
     6841 ggggaaatat ttgccgggat ttgcgtgctc gtggctctgt tcgtcgccat cgctgcgtta
     6901 catattattc aggcgaaaaa gatcgacgag gtgaacagaa gtatcgcaaa ggctgagaaa
     6961 aggatcaaga cgctcgaagc cgttaagaaa aaggtggatg atttcaaggc caagaacgcg
     7021 gagctcaaca ggaggatcga gatcataggc gtgctcgaga aaaaccgtac cgggcctctt
     7081 tacgttatgg acgctctgtc acgcgcgatg cccaacaagg cgtgggtaga cgaatttacg
     7141 gagaagggtg ggcagaccaa gctcgtaggc atcgccgaca acgaatttac cgtggcggat
     7201 ttcatgcagg cactccagtc ctccccttat tttgtcggag tggagctggg tgtgataaaa
     7261 aaaaccgagc tgcgaaagct cgacctgagg acttttgtta taaatacgag gctcgattat
     7321 acaggcgggg ccgggaagca gaaaaaggag caggaaccta aggcgcccgg acaggacacg
     7381 gggcgcaaga aggcggtgac gcaataatgt ctttcctgga cgattatacc gaaagactga
     7441 gcgagcagcc cctgcatgtc aaagcgggca tcctgcttct cgtagttgcg gtgatatcag
     7501 ccgcttactg gtatttcttc tggtcgccca aagcggaagt gcttgagcag gcgcagaaaa
     7561 aattgaaaca ggaagagatc aaggtgaagg aatacgaagc catagcggcc gagctgccca
     7621 agttcgaaaa ggagtatcaa agactcgaaa aggaattcga gctcgtcgcg cgcaagctcc
     7681 cgaaggacaa ggagataccg gctctgatag acggcgtata ttcggatata gccgcctcta
     7741 atctcgattc gataatattc gctccccagg ctcaggtcac gaaagagatt tacgccgaga
     7801 taccgatcga gatggaggtc atcggcacct actttaacct ggccgatttc ttcgaccgca
     7861 tatctaggct tccgaggatc gtgaatgtga gggacctgca gcttacgcgc aacgacatcc
     7921 gcggggggac cgtgatactg aatgcaaaat ttaacgtcgt tacgttcagg cttttacctc
     7981 cgcaggaggc aaagcctcag gatactaaaa aaactaaggg taagaaacgc ggtaaatgaa
     8041 tatgacggcg agcgtatgcg gcatgattaa aacgaaatac ggactattta aattacttgt
     8101 cgtcctgctg ctcgtgctct ggtctctaca ggcgggcacg gcgcaggaag cggaagcccc
     8161 tgcggacgat acgctcagcc agggcctcaa tgtgattaaa gacgcaaaag ataataagct
     8221 cgtaacgcct gaagaaaggt ctgctgaggc ggtcggagaa gaagccccct ctcctgagca
     8281 gagccccgcc cccggtgagg gacagggtgt accggccgct ccggaagccg ggaggggaga
     8341 cgcgctcatg agccgtgacg aaaagcctcc ctacgattcg actgggatgc gcgatccgtt
     8401 caagcctttt ataaagctcg ccgatactcc cgccgcgcct tccctggtac tgcgtccgcc
     8461 gatacagcgg tacccgctca accagttcag aatcacgggg atagtatgga taggagggaa
     8521 gccgcaggct atggtcgtcg atccggaagg gaatacgtat ttcctcggag tgggcgataa
     8581 gatagggagc agtgacggcg aaatactcga ggtaagacag gacgggatcc ttgtggagga
     8641 gaaaacgagg ctcgaaaacg tatacggaga ggtaaagttc gaagtgaagc agtcggtgct
     8701 tgcgtttcag aacgaagaat gatgagggga attttatgca ggaggttaag ttaaggagga
     8761 tggattaatg agaatcctga taatatcaat cgcgcttgca gcctgtatcc tttattcggg
     8821 actcgaggct catagcagtc aggccggaca aaattacagt agtaattacc ctccggccgc
     8881 cgcgcagggg gctgacggcg gggaatcagt tgctacccct acgccggccg agcttgccca
     8941 ggtcgatgcg gccacggtaa ccgtcccgca gaacactacg gctaacatgc ccaatccctc
     9001 cgatgaatat aaccacatcg agagtattgg gttcgaggtc cagcagggca tgggcattgt
     9061 caccgtgggc acgtcctgga acacacaata cgagagactc caggacgagg ataagaagat
     9121 aactctcaaa ctcaaggacg tcatactcgc cgacaagttt caggtctccc gcgacgtgag
     9181 cgatttcgac agcccgatca acttcatttc gtccttcaga gaccccatca agcctaacga
     9241 cgtaataatg gtcatagagc tcaaggagaa ggacgttccc gtggatatcg gacagaacgg
     9301 gaacaccatc accatgaatt tcggggaaac gagggagcag gagaaaatag ccgccgaaaa
     9361 aggagaagtc gaggtcgcgg tgaagcccga ggacctgtat gcgttcgatt tcaggttaac
     9421 ctcgatgccg ggcgcggtga agacatacaa gggccagctc gtctcgttcg atttcaagga
     9481 cgcggacatc agggacgtgc tccggataat ttcagacata agcgggctga acatgataat
     9541 ctcacggaac gtgtcgggca cggttacgct caagctgacg aacgtcccgt gggaccaggc
     9601 gctcgacgtt attctcgagg acgccgggtt aggcgcattc atagacggga acgtgctgag
     9661 ggtcgcgcct ctcgggactc tccaggcgag acagaaggcg gtaaagaaag cggccgagac
     9721 gagcgaggag ctcgagccgc tcatcacgaa gcaggtgttc gtcaattacg ccacggctga
     9781 ggagctgata ccgctcgtgg agcctctcct tagcgataga ggggagctca gggtcgacgt
     9841 gcgcacgaac agcatactta tcaacgatac aggggtcaga gtggcccaga tagagggcct
     9901 ggtggccgac ctcgatacga gaacgccgca ggtattgata gaatccagaa tagtacaggc
     9961 gactcttaat ttcacgcgcg agctgggaat acagtggggc ttcgattaca gggcatcagc
    10021 ggcgaccgga aacccgacgg gagcgacgtt cccgtcgagc gtcgaggtgg gcggtacaac
    10081 gaccgggagc cccttcggga cgaccggcga caactttatc atagacctgc ctgcggcggc
    10141 cggtacggga gcgggcggag tcctgggact cgtattgggc agcctgaccg gggcatacga
    10201 cctcgacctc aggctatccg cgctcgaggc cagaggggac ggcagagtgc tgtcttctcc
    10261 caaagtatta acgctcgaga acacgcccgc caggatagaa cagggcgtct cgataccttt
    10321 cctgagcgtg tcggcggcgg gaacgcagac tcaattcgtc gacgcaacgc tcagcctcga
    10381 ggtaacgccc caggtgacga acgacaacag gatattgatg acgatagtag ttacggataa
    10441 cagaccggat acggctctga ccggggcggg aggacagccc agcatcagga ggaacgaggc
    10501 tacgacccag gtcctcgcga gcgacggcga gactatcgta atcggcggta tatttaccag
    10561 aaacgtaacc gatacggaca acagtctgcc gtggttctca agactgccgc ttctcggatg
    10621 gcttttccag aggtccgcga cgactgatga gaggagagag ctgatagtat tcattacgcc
    10681 acgaataatc agatgaaaat acattgcaat acgagttcag gaggatgatg tgatgaacag
    10741 aaaaataatg
//
LOCUS       DASSJH010000119         5552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_352000, whole genome shotgun sequence.
ACCESSION   DASSJH010000119 DASSJH010000000
VERSION     DASSJH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352000"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..895
                     /locus_tag="VFJ67_02605"
     CDS             122..895
                     /locus_tag="VFJ67_02605"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7289303.1"
                     /translation="MIYTKVAENAAREAGEVLKANLGKVREITFKGKNSLVTEVDKLS
                     EDIIIRNIKRSFPSHDIFAEESGRHSTSSEYVWIIDPLDGTTNYAHGYPFFSISIALE
                     IEGVVKAGVVYDPVKDEMFSAELGQGAYMNGEPIRVSGSVRLDESHLVTGFVHDDPHM
                     VEENLRHFANFIWKARAVRRDGSAALDLCYVACGRFDGFWELGLNPWDTAAGMLIVEE
                     AGGRVTDFIGGEYSIYVREILASNSSIHSEMIDVLSLGR"
     gene            1376..2425
                     /locus_tag="VFJ67_02610"
     CDS             1376..2425
                     /locus_tag="VFJ67_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HET7289304.1"
                     /translation="MEKSGLKNAALLLTIAVFYNILTFLCAHADDNEVKETRYYYAGT
                     IGDNLPIQMELIFDSGDVTGSYMYDKVGIPITLTGSLDSGTAVLSLVEQDEKGLKTGT
                     FKGSVISEGKDFGNYIEGKWSKANGLTTLPFKLTKVADFVSTGLSVKGKYEATYMYPS
                     FVTGTKAGREISERLGKEAASEKKKFADQAEEFFGSQESAGEWQEDYSYSIQYYSPEL
                     ISLSGEVFSYTGGAHGNTFYVSSNYWIKEDKALLLSLADLFAPKSNYIKALSDFCIRD
                     LRKQQAGWVLNGELKELKAEDLSAFAVSPGGVTFAFAPYVAGPYVEGPYFVTVGYGEL
                     KGALKPDGPLRQFLK"
     gene            2434..2988
                     /locus_tag="VFJ67_02615"
     CDS             2434..2988
                     /locus_tag="VFJ67_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289305.1"
                     /translation="MKRLIRARVLVERAVWAIITGSVIIYFFLAYLMTGQNAGAGARG
                     VSGAEPLFYIAVAAAAVFSVVYRRRSFSDERMRAILNKFDTDAFTDEPVTERAGPDGM
                     DNISAFNESEKRAFFLLNELQKSSIINLILNEIVIMIGFVLSFLSGDFMKIIPFGIAS
                     LVLCLWMFPRTESALQKAQNIFSR"
     gene            3070..4734
                     /locus_tag="VFJ67_02620"
     CDS             3070..4734
                     /locus_tag="VFJ67_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7289306.1"
                     /translation="MAASRNSNTDVLIVGAGPTGLTMACELARHGVTPRIIDKSPTPS
                     VHSKAFGIHARTLELFETMGIVETVLGQGNICNGFDMYNRGKPLAHVNFDTLESRYPY
                     FLILAQSDTERILNAHMESFGIRVEREKELVGIERTNAGITARLKLEDGSEEFIDCWY
                     VAGCDGAHSTSRHLLDLEFKGAPYPNYWLLADCDLDWKYPLHHLSVFIHPSGVTAYFP
                     LYSDRGRLMFELPDATIDMEMAEPTIEDVRRHMKERQIDYKSVGSPNWLAYFKLHHRM
                     VDKYGEGRVFLCGDSAHIHSPIGGQGMNTGIQDAYNLAWKLALVLKGNSPENLLDSYN
                     PERRRVGESVVGLTDMATRMVGIHNPVLAAIRNKLVGTISKLDPVQEKLLTTLSQIEV
                     NYRESPIVGERWYQPDAIEGFHGYRHDLVAGERVKDYPLGSLDGKGGARLYDLFRDVR
                     HKLLLFTGAEPEDMELNELRKIRSSIGPEYGDIIDIHVIAGTDDLPADLKTMGPVWRD
                     KDLVMHRDFGAAKASLYLIRPDSYIGFRNQPASLSDLREYLPVIFL"
     gene            4790..5536
                     /locus_tag="VFJ67_02625"
     CDS             4790..5536
                     /locus_tag="VFJ67_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HET7289307.1"
                     /translation="MPEYKAFFHFHHELRDFFTEPGTALSVQYGFDGKPSVKDAIEAI
                     GVPHTEVDTIVVNGAPVGFGYHLADGDAVSVYPASFGPDLPSGIPLRGEPHPAFVVDV
                     NLGKLARLLRMLGFDAAYRNDFNDHEIAELAEGEGRIVLTRDRRLLRFKVIEHGYWLR
                     SDDPRVQISEVIRRYGLGPLIKPFKRCLVCNGRIEPVEKEKVIERLEPRTKIYYEEFY
                     ICSGCSKIYWKGSHYDHMNETLGILWDSNR"
BASE COUNT         1384 a         1358 c         1541 g         1269 t
ORIGIN      
        1 ttacttcagc gcctgttgtc tgtcccgaat aatctcaata tcgacagggg aaatgtatga
       61 gtgcgtgccg tatttcatgt acggtactga tcgaacgtct tctgcctgga gtgtgtttgc
      121 cttgatctac acgaaagtcg ctgaaaatgc cgcgagggaa gcgggagagg tgttaaaagc
      181 aaacctgggt aaagtgagag aaatcacttt taagggtaag aacagcctcg tgacggaggt
      241 cgacaagtta tccgaggata taatcatccg aaacataaag aggagcttcc cgtcacacga
      301 tatattcgcc gaggagtccg ggagacattc gacgagctcc gagtacgtat ggataatcga
      361 cccgctcgac ggtactacca actacgcgca cggttatccg ttcttttcca tatcgatagc
      421 gctcgagata gagggtgtcg tgaaagccgg tgtcgtttac gacccggtaa aggacgaaat
      481 gttctcggcg gagctgggtc agggcgcata tatgaacgga gaacctatac gcgtatcggg
      541 ctccgtgcgg ctcgacgaga gccatctggt cacgggtttc gtccacgacg acccgcacat
      601 ggtcgaggag aatctgagac acttcgccaa cttcatatgg aaagccaggg ctgtaaggag
      661 ggacggctcc gcggcgctcg acctctgcta tgtcgcgtgc ggcaggttcg acggtttctg
      721 ggagctcggt ctcaatcctt gggacacggc cgccggcatg ctcatcgtcg aggaagcggg
      781 tggtagggtg acggatttca tcggcggcga atacagcatc tatgtcaggg agatactggc
      841 ttccaacagc tcgatccact cggaaatgat cgatgttctg tctctcggca gatagaacaa
      901 atagcgcatc aacactgcat cgggaaaaaa taagggtgaa ccttgaattg cttcaaagat
      961 tcacccttta gaggatgggg aatatgttat ggccgtgatt aattcaatct gctacattac
     1021 agaataatta cgttgtaacg aaaaaaacgg atctgttttc cctgaattca atgtccgtta
     1081 atttcatata tacgcattgt ttttattatg caacgcgtgt gccaaaaggc ccgaaagtgt
     1141 gcaataggct gtggttattg caaaaatatg taaataccag ggtgtgagta tataacggac
     1201 agtcgatatg tgtaaaaatt tggtcatttt ttgacaaaag ttgtcgaaaa ggagtgtgac
     1261 ggattatgat atgatatgac aaacggagga ttatgcccgt atgttatacg aaacattaat
     1321 ctttcttgct catagaaaaa gtgtaataat aataaattgt catcaagggt gataaatgga
     1381 gaagtccggt ctcaaaaacg ccgctttact gctcacaatt gcggtgtttt ataatatttt
     1441 gaccttctta tgtgctcatg ccgacgataa tgaggtcaag gaaactcgtt actattatgc
     1501 cggtacgata ggcgacaacc tcccgatcca gatggagctg atattcgact cgggggacgt
     1561 aacgggctcc tatatgtatg acaaggtcgg catacccatc actttaacgg ggagcttgga
     1621 cagcgggaca gcggttctat ctctggtgga gcaggatgag aaaggactga aaacagggac
     1681 atttaagggt tccgtaataa gcgaagggaa ggatttcggg aattacatag aaggcaagtg
     1741 gtcgaaggcg aacggactaa cgactctgcc gttcaagctc accaaggtag cagatttcgt
     1801 ttcaaccggc ttgagcgtta aaggcaaata tgaagcgacg tatatgtacc cttccttcgt
     1861 gacgggcacg aaagcgggcc gtgagatatc ggagaggctc ggaaaggaag ccgcctcgga
     1921 aaagaaaaaa ttcgcggatc aggcggaaga gttcttcggc tcacaggaat cggccgggga
     1981 atggcaggag gattacagct actcgatcca gtattattct ccggaattaa taagtctatc
     2041 cggagaggtg ttctcctata cgggcggagc gcacggcaat acgttctacg tctcgtccaa
     2101 ttactggata aaagaggata aggcgttact tctgagcctg gccgatttgt tcgcgcccaa
     2161 gtcgaattat atcaaagccc tctcagattt ttgcataagg gaccttcgaa aacagcaggc
     2221 gggctgggta ttgaacggag agctcaagga gctcaaggcg gaagacctta gcgcgttcgc
     2281 ggtctcgccc ggcggcgtaa ctttcgcttt cgctccttat gttgcaggcc cctacgtcga
     2341 aggaccatat ttcgttacgg tcgggtacgg ggagctcaaa ggagccctca aacccgacgg
     2401 tccgttaagg cagtttctca aatgaccccc cgattgaagc gtcttatccg cgcccgggtg
     2461 ctggtcgagc gcgcggtctg ggcgataatc acgggttcgg tcataattta tttcttcctt
     2521 gcatacttga tgacaggaca gaatgcgggc gcgggagcca ggggcgtgag cggcgctgag
     2581 ccgctgtttt acatagccgt tgcggccgct gccgtgttct cggtcgtata tcgccgccgg
     2641 tcgttttcgg atgagcgcat gcgggcgata ttgaacaaat tcgataccga tgcgtttacg
     2701 gacgagcccg taacggagcg cgccggtccg gacggcatgg ataacataag cgcgtttaac
     2761 gaatcggaga agagagcgtt ttttcttttg aacgagttac agaagtcctc gataattaat
     2821 ctcatactca acgagatagt gataatgata ggcttcgtac tgtcttttct gagcggcgac
     2881 tttatgaaga tcataccgtt cgggatagcg tctctcgtat tatgtttatg gatgttcccg
     2941 agaacggaat cggcgttaca aaaagctcaa aatatattct cccgataatc atcgataaat
     3001 tttgcgttaa tattacgacg ggcatcggcg ccgtaccgcc gatataatat gcccgtgacg
     3061 gagattccaa tggctgcttc ccgtaattca aataccgatg tgctgatagt gggggctggc
     3121 ccgacgggcc tgacaatggc gtgcgagctc gcgcggcacg gggtaacgcc gaggatcatc
     3181 gacaagtctc ccactccttc ggttcattca aaggccttcg gcatccacgc gcggacgctc
     3241 gagctctttg agacgatggg catcgtcgaa accgtgctcg gacaggggaa tatctgtaac
     3301 ggcttcgata tgtataaccg cgggaaaccg ctcgctcacg ttaacttcga cactctcgag
     3361 agcagatacc cgtatttcct cattctcgcc cagagcgata ccgaaaggat attgaacgct
     3421 catatggaat ctttcgggat cagagtcgag agggaaaagg agctcgtcgg aatcgagcgg
     3481 acgaacgcag ggattaccgc gcggttaaag ctcgaggacg gttcggaaga atttatcgat
     3541 tgctggtatg tagccggatg cgacggagct cacagcacat cgcgtcatct cctcgacctc
     3601 gaattcaagg gcgctcccta ccccaattac tggctccttg ccgactgcga tctcgactgg
     3661 aaatatccgc tccatcatct ctccgtattt atacacccct ccggcgtgac ggcgtatttc
     3721 cccctctaca gcgacagggg gagactgatg ttcgagctcc cggacgcgac tatagatatg
     3781 gaaatggccg agccgactat cgaagacgtt agacggcaca tgaaggagag gcagatcgat
     3841 tacaagagcg tcggctcccc caactggctc gcctacttca agctccacca ccggatggtc
     3901 gataaatacg gtgaagggag ggtgttttta tgcggagact ccgcgcatat ccacagccct
     3961 atcggcgggc agggaatgaa tacgggtatt caggatgcgt ataacctcgc gtggaaactc
     4021 gctctcgtgc tcaaggggaa ctctcctgag aacctccttg acagctataa tcctgaaaga
     4081 cgccgcgtgg gggagagtgt cgtgggattg acggacatgg ccacgagaat ggtaggtatc
     4141 cataatccgg tccttgccgc tatccgtaat aagctcgtag gcacgatatc gaaactggac
     4201 cccgtgcagg agaagctcct cacgaccctg tcccagatcg aggtcaatta cagggagagc
     4261 cccattgtcg gggaaagatg gtatcagccg gacgcgatcg aggggttcca cggttacagg
     4321 cacgacctcg tagcaggaga gcgggttaaa gattatcccc tcgggagcct cgacggaaag
     4381 ggcggcgcgc ggctctatga tctattcaga gacgtaaggc acaagctcct ccttttcacg
     4441 ggcgcagagc ccgaagacat ggaacttaac gagctgagaa aaatacgcag ctccatagga
     4501 cccgaatacg gggacatcat cgatatccac gtgattgcag gcacggacga ccttcctgcc
     4561 gatttaaaaa cgatgggccc tgtttggaga gacaaggatc tggtgatgca cagagatttc
     4621 ggcgccgcca aagcgagcct ctatctcatt cgccccgaca gctacatagg gttcaggaac
     4681 cagcccgcga gccttagcga cctgagagag tacctccccg ttatattcct ctgagcgttt
     4741 gctatacttt ctcttatatt cataacggaa tcgacggcag ggtcgcaaca tgcctgaata
     4801 caaggcgttc tttcattttc atcacgagct ccgcgatttc tttacggagc ctggtacggc
     4861 tttatcggtt caatacgggt tcgacggaaa gccttccgtg aaggacgcga tcgaggctat
     4921 cggggtcccc cacaccgagg tcgatactat cgtcgtgaac ggcgcccctg tcggcttcgg
     4981 ctaccatctc gcggacggtg acgccgtatc cgtttatccc gcatcgttcg gccccgatct
     5041 gccttccggg atcccgctcc ggggagagcc gcatccggca ttcgtagtgg acgtgaatct
     5101 agggaagctc gcgaggctct tgaggatgct cggcttcgac gccgcataca ggaacgactt
     5161 caacgaccat gaaatagcgg agctcgccga gggcgagggc cgcatcgtgc tcacccgtga
     5221 caggcgtctc ctgagattca aggtaataga gcacggctac tggctcaggt ccgacgaccc
     5281 ccgtgtgcag ataagtgagg ttatccggag gtacggcctc ggccctctca tcaagccgtt
     5341 taagaggtgt ctcgtgtgta atggccgtat tgagcccgtt gaaaaggaga aggtcattga
     5401 gcgcctcgag cccaggacaa aaatttatta cgaggaattc tatatctgct ccggctgcag
     5461 taaaatttac tggaaagggt ctcactacga ccacatgaac gagacgctcg gcatcttatg
     5521 ggattccaat cgatagtgcg aaaaaccgga ga
//
LOCUS       DASSJH010000120         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_135549, whole genome shotgun sequence.
ACCESSION   DASSJH010000120 DASSJH010000000
VERSION     DASSJH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135549"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFJ67_02630"
     CDS             complement(<1..439)
                     /locus_tag="VFJ67_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HET7289308.1"
                     /translation="MTVRTRFAPSPTGSLHIGGARTAIFNWLFARHGGGDFILRIEDT
                     DRSRSTEESIHEITDALQWLGLDWDEGPFRQSDRLDIYRELAEKLIASGHAYKCYVTP
                     EELEEKRKEAQERGEVFHYRREWSDRNASPGKPYAIRMLTPDHG"
     gene            748..1290
                     /locus_tag="VFJ67_02635"
     CDS             748..1290
                     /locus_tag="VFJ67_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289309.1"
                     /translation="MNKGIFLLLSAALLLGSCSIGKFVGYYASAGTDDDGKLDGQVYT
                     TDKTSYMIGTLPESWKRIRIEGGDLAFSNGVDDSTITVNSTCDERKMKYSLKALSESL
                     ITGIKDKEAQSREDIQIDGQPALMTIYTGSLNNAPVKIETRVFKKNECVYDFTYASSP
                     SKFDAEARVFDEFISQFRVL"
     gene            1287..2069
                     /locus_tag="VFJ67_02640"
     CDS             1287..2069
                     /locus_tag="VFJ67_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7289310.1"
                     /translation="MIIYNYVSRLGSYCVSVIEQLGEVWLFLYGAIFASFTRPFNFRL
                     VLEQVDEIGFKSIPIVIASAMAIGMVMVVQLAWGFAWFGAKGVVGPVVSLSFVRELGP
                     VVTSLLVGGRVGSGITAEIGSMKVTEQIDAIRTLGADPIRKLVVPRLVASVICFPFLA
                     VISDFTGILGALLMANIDLDVKPRLFISSILEWVTIDDFISGISKTFFFGIIVAITGC
                     YIGMKAEGGTQGVGRATTSTVVVALLLIIIGDFFLTKLFLLL"
     gene            complement(2143..2646)
                     /locus_tag="VFJ67_02645"
     CDS             complement(2143..2646)
                     /locus_tag="VFJ67_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289311.1"
                     /translation="METIGKVLSLTLLTIFGSAAATVYLVDSDIMPSFFGKPAHVYTS
                     DRTEQPDENADFYKVPERRERARESLHYAVVEKETGEIGEYNNIKPIWGQSYETDTVS
                     ARRNAERAREIARSNSLASITQNIDYWNDQFSIAQREGKQAAAQSALRNYTEYKEALE
                     IKQSASR"
     regulatory      complement(2764..2852)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3141..>4016
                     /locus_tag="VFJ67_02650"
     CDS             3141..>4016
                     /locus_tag="VFJ67_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET7289312.1"
                     /translation="MNDKMMEGILFTDQYQLTMAQLYYRYGLHETDAQFDHFFRNYPD
                     YGSHKAGYCVNAGLEWLVNWMKEAAFGDEEISYLRTQTGQLGEPLYTEDFLDWLRKHG
                     NFGGITLLSIPEGRVVHPTVPVTVVQGPLPMAQVLESALLNKLNYQILIATKAARIRE
                     AGRGQPILEFGMRRGPDLGVNAGIRAALIGGADFSSTVGISHLLGFPPKGTHAHSMVQ
                     VFMALGEGELGAFRAYAEVYPDDCLLLVDTINTLESGVPNAIKVFEELRRKGHKPLGI
                     RLDSGDLAYLSIQSAR"
BASE COUNT          866 a         1018 c         1115 g         1017 t
ORIGIN      
        1 tcccgtggtc aggcgtgagc atgcgtatgg cgtagggctt gccgggcgag gcgttcctgt
       61 ccgaccattc gcgcctgtag tggaagacct cgcccctctc ctgcgcctcc tttctcttct
      121 cttcgagctc ctccggagtc acgtaacatt tgtatgcgtg tcctgacgca attaatttct
      181 cggcgagctc cctgtatatg tcgagcctgt ccgattgccg gaacggcccc tcgtcccagt
      241 cgagccccag ccattgaagc gcgtccgtta tttcgtgtat cgattcctct gtcgagcggg
      301 agcggtcggt gtcttctatc ctgagtatga agtccccgcc gccgtgtctc gcgaagagcc
      361 agttgaaaat ggctgtcctc gccccgccga tatggaggga gcccgtgggg ctcggggcga
      421 atctggtcct gacggtcatg ggggtaaaat gttaatttag agtcacaggc gagtcaaata
      481 agcctttaca gcacggtgaa agaacggcga ccagatccgc gcggccctgc tacttaatcc
      541 ctgtttttac gtagggtgtt tgtgctttat ttgtatataa aattctgagg ctcgttttat
      601 ttccgcaaaa gaggggatac aattactcaa gtttgagcgt tcttttgttt ttttaaggtt
      661 tcgagtatta ttgagaaccc gtcggcccgc gtgttgaggc ccgcctgagc aaggtacgga
      721 aaatcttgat acaagttaaa ttatccaatg aataagggca tatttttatt attatcggct
      781 gcgctcctcc tcggttcctg ctctatcgga aagttcgtgg gctactacgc ctcggcgggt
      841 acggacgatg acggcaagct cgacggacag gtttacacga cggacaaaac gtcctacatg
      901 atcggcacgc ttccggaaag ctggaagagg ataaggatag agggcggcga cctcgcgttc
      961 agcaacggcg ttgacgattc aacgattacg gtgaattcca catgtgacga gcgcaagatg
     1021 aaatacagtc ttaaggctct ttcggaatct cttataaccg gtataaagga caaggaggcg
     1081 cagtcgagag aggatattca gatagacggg cagcctgcgc tcatgacgat atatacgggg
     1141 agcctgaaca acgctccagt caagatagag acgcgtgtat ttaaaaagaa cgaatgcgtg
     1201 tatgatttta catatgcctc gtctccgtcg aagttcgatg cggaagcacg ggtgtttgac
     1261 gaattcatat cacagttcag agtcttatga ttatatataa ctacgtatcg cgcctcggtt
     1321 catactgtgt ctccgttata gagcagctcg gcgaggtgtg gctctttctc tacggggcca
     1381 tctttgcgag cttcacgcgc ccgtttaatt tcaggctcgt tctcgagcag gtagacgaga
     1441 tagggttcaa atctataccc atcgtcatag cttcggccat ggcgataggg atggtcatgg
     1501 tcgttcagct cgcgtggggc ttcgcatggt tcggagcgaa gggggtcgtc ggtcccgtcg
     1561 tatcgctctc gttcgtcagg gagctcggtc ccgtcgtaac gtcgctcctg gtgggcggca
     1621 gggtgggctc cggcatcaca gccgagatag gctcgatgaa ggtgaccgag cagatcgatg
     1681 ccataaggac gctcggcgcg gacccgataa ggaagctcgt agttccgagg ctcgtggcgt
     1741 ctgtcatttg cttcccgttc cttgcggtga tatccgactt caccggtatc ttgggcgcat
     1801 tgcttatggc gaacatagac ctcgacgtaa agccgaggct cttcatatcg agcatactcg
     1861 aatgggtgac catcgacgat ttcattagcg gcatttcgaa gacgttcttc ttcgggatca
     1921 tcgtggccat aaccggctgc tatatcggaa tgaaggccga gggcggcact cagggcgtgg
     1981 gaagagcgac cacatcgacc gtcgtagtgg cgctcctcct cataatcatc ggggattttt
     2041 tccttacaaa gcttttcctt cttctctaat aatccggaat atcggacggg ctcaaaagcg
     2101 ggcggcgatc cggcgcgggc gcctctgtgc gtaagggatt atttatcggg aagccgattg
     2161 tttaatctcg agcgcttctt tatattccgt ataattcctg agcgcgcttt gtgcggctgc
     2221 ctgcttacct tctctttgcg cgatcgagaa ctgatcgttc cagtaatcta tgttttgagt
     2281 tatcgaagct agggagttgc tccgggcgat ctcccgtgct ctctccgcgt ttctccgggc
     2341 tgagaccgtg tcggtctcgt agctctgtcc ccagatcggt tttatattat tgtattctcc
     2401 gatttcccct gtctccttct caacgactgc gtaatggaga ctctcccgcg ctctctctct
     2461 tcgttcgggg accttgtaga aatcggcgtt ttcatccggc tgttcggttc tgtccgacgt
     2521 atagacatgt gcgggctttc cgaaaaaaga aggcattata tccgaatcga ccagataaac
     2581 ggtcgcggcc gccgagccga agatcgttag aagggttaag ctcaatacct tgcctattgt
     2641 ttccatccgg ctatgcacct cactgccgtg tacaaaacgg gttcggtcta agtcagacgg
     2701 gagctgtgat cgggaaacgg attcgggaag gtcggtatta ttcgagaggc tctatttttc
     2761 cgtttcggcg gcatggcggc cattgggttt aatctccctt taggcccgtt gctttgcgtc
     2821 ccatcctttc gagtggtttg ccgtttcgta cgcaacacaa taatgactga aatcacataa
     2881 tccgtatcgt atttataatg taaagaatat aaggaatgac gcatattgtc aatacacttg
     2941 aaattcgtca gcgatctccg caaattgtta caattgtatt gttccgcggc ttcatttgtt
     3001 gatgcggact aacccccgct tatgggcaat atgttgactt agggtaaaaa taattctata
     3061 cattaataac ggtcccggtt ttatgcggcg gccggaaaat tcccgctaag aaattaaaaa
     3121 tccttggtta aagagtagat atgaatgaca agatgatgga agggatactc tttaccgacc
     3181 agtaccagct cacgatggcc cagctctatt acaggtacgg gcttcacgag acagacgctc
     3241 agttcgacca ctttttcaga aattatcccg actacggttc ccacaaggcg ggttactgcg
     3301 tgaacgcagg gctcgagtgg cttgtcaact ggatgaaaga ggccgccttc ggagacgagg
     3361 agataagcta tctccggacg cagacgggtc agctgggcga gccgctatat acagaggatt
     3421 tcctcgactg gctcaggaaa cacggcaact tcggcggcat aacgctcctt tccatacccg
     3481 agggcagggt cgtccatccg acagtcccgg taactgtcgt ccagggcccg cttcccatgg
     3541 cgcaggttct ggagtcggcg ctcctcaaca agctcaacta ccagatactc attgccacga
     3601 aggccgcgcg cataagggag gcggggcggg gccagcctat actcgaattc ggaatgagga
     3661 gagggcccga cctcggggtc aacgccggca taagggcggc tttgataggg ggggccgatt
     3721 tctcttccac ggtaggtatc tcgcacctgc tcggttttcc gcccaagggc actcacgcgc
     3781 acagcatggt gcaggtgttc atggcgctcg gcgagggcga gttaggcgcc ttcagggcct
     3841 atgcggaggt atatcccgac gactgcctgc tgctggtgga tacgataaac acgctcgaga
     3901 gcggggtgcc taacgcgata aaggttttcg aggaattgag gagaaaaggg cataagccgc
     3961 tcggcatcag actcgattcg ggcgacctcg cgtatctgtc catacagtcg gcgagg
//
LOCUS       DASSJH010000121         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_460572, whole genome shotgun sequence.
ACCESSION   DASSJH010000121 DASSJH010000000
VERSION     DASSJH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460572"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFJ67_02655"
     CDS             complement(<1..694)
                     /locus_tag="VFJ67_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET7289313.1"
                     /translation="MEGKRINVSGLGTREKAGQVIMPRLDFRGPDPLPLARRLVSEYG
                     VGGFIVFGGDKQTVKSAAAELSSISAIPLLFGLDAERGVGQVLPDGTLFPSAMSLGAA
                     GDEALAYEEALMIALEMKECGFNLLFAPVLDVNTNTENPIINTRAYGDDPGLVSRMGT
                     AFARGAQDGGVLACGKHFPGHGGTGLDSHEVMPVEDSELSELESSSLIPFRNAARAGL
                     AAIMTAHVAFPKI"
     gene            complement(700..1881)
                     /locus_tag="VFJ67_02660"
     CDS             complement(700..1881)
                     /locus_tag="VFJ67_02660"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HET7289314.1"
                     /translation="MLEKLRSLLDKKEKLVVGLISGTSMDGIDAALLRISGSGTGTRI
                     EMINFICVPYEENIRRGIDGLSHNCSAADISSLDFLIGAAFANAALKVIDGSGTGRDS
                     VDLIGSHGQTVFHNPPSRKEGVPSTLQLGELDVIAESTGITTVGDFRTRDIAAGGEGA
                     PLVPYPDYLLFNKPGEVHIAQNIGGIANAAVIPGRLEDVIAFDTGPGNMLMDRVINIS
                     TGGKKRFDEDGEYASRGEADEKLLKELLSDRYFMLPPPKSTGEELFGKERAEALVSRV
                     NKGQIPLIDLMATLIELTVKSISLSYERFIFPHHNVNEIIFSGGGCRNPVLMKRLRDE
                     FPGIGISTTDDYGVPSDAKEAVAFAILANELVSGNAGNLTGVTGASHRTPLGKIVIGR
                     L"
     gene            complement(1944..>2262)
                     /gene="murQ"
                     /locus_tag="VFJ67_02665"
                     /gene_synonym="yfeU"
     CDS             complement(1944..>2262)
                     /gene="murQ"
                     /locus_tag="VFJ67_02665"
                     /gene_synonym="yfeU"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the cleavage of the lactyl ether moiety
                     of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form
                     N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate;
                     involved in MurNAc dissimilation pathway; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HET7289315.1"
                     /translation="STRMKAGTATKMVLNMLTTAAMVKLGKTYGNLMVDVLPRSAKLR
                     DRAVRIVTEIAGVDEREAGRLLKKSGWDVKASVVMAKKGIGYKKAKDLLFKNNGFLRK
                     ALS"
BASE COUNT          445 a          680 c          608 g          529 t
ORIGIN      
        1 cgattttggg gaaggccacg tgggcggtca ttatggcggc gaggcccgcc cgggcggcgt
       61 tccggaacgg tatgaggctg gatgactcga gctccgatag ttccgaatcc tctaccggca
      121 tgacttcgtg agaatcgaga ccggtgcctc cgtgtcccgg gaaatgtttc ccgcaggcaa
      181 gcaccccgcc gtcctgagcg cctctcgcaa aagcagtccc catacgggag acgagcccgg
      241 ggtcgtcccc ataggcgcgg gtgttgatta tcgggttctc cgtgttggtg tttacgtcga
      301 gcacgggtgc gaataacagg ttgaatccgc attccttcat ctcaagggct atcatcagcg
      361 cttcctcgta cgcgagagct tcgtcccctg ctgctccgag cgacatggcc gacgggaata
      421 gagtcccgtc tggcagcacc tgccctaccc cgcgctcggc gtcgagtccg aataataaag
      481 gtatggctga tatcgagctc aactccgcgg cggcactttt tacagtttgt ttatccccgc
      541 cgaaaacgat aaacccccct acgccgtatt cgctgacgag tcttcgggcg agggggagcg
      601 gatcgggccc cctgaagtcg agcctgggca taattacctg cccggctttt tcacgcgtcc
      661 cgagacctga cacatttatt cgttttcctt ccattccgtt cataatctgc caatgactat
      721 ctttcccaaa ggggtcctgt gcgaagcgcc cgtgactccg gtgaggttcc cggcgttgcc
      781 cgagacgagc tcgttggcga gtatcgcgaa ggccaccgcc tccttcgcat cggaggggac
      841 gccgtaatca tccgtggtcg agattccgat gccgggaaac tcgtctctca gccttttcat
      901 aaggacaggg ttcctgcacc cgccgccgct gaaaattatc tcatttacat tgtggtgagg
      961 gaaaataaac ctctcgtaag atagcgatat ggatttgacg gtaagctcga tcagcgtcgc
     1021 catcaagtca atgagcggaa tctgcccctt gtttacacgc gaaacaagcg cctccgcccg
     1081 ctccttgccg aagagctcct cgcctgtaga tttcggcgga ggcaacatga aataacgatc
     1141 ggacaagagc tctttaagga gcttctcgtc cgcttcaccg cgggacgcgt attccccgtc
     1201 ctcatcgaat cgcttcttgc ctcctgtgga aatgttgatc accctgtcca tgagcatgtt
     1261 tccggggcct gtatcgaacg ctatcacgtc ttcgagacgc ccgggtatga cggccgcgtt
     1321 cgctatgccg ccgatattct gggctatgtg cacctcgccg ggcttattga acaggagata
     1381 atccggatag ggcacgagcg gcgcgccctc gccgcccgcc gctatatcgc gcgtcctgaa
     1441 atcaccgacc gtggtaatcc cggtcgactc cgcgattacg tctaactccc cgagctggag
     1501 agtggacgga acgccttcct tacgggaagg cgggttgtgg aaaacggtct gaccgtggga
     1561 gcctatcaga tccacactgt ctctgccggt cccggaaccg tcaatgacct ttaatgccgc
     1621 attcgcaaag gccgcaccga taagaaaatc gagacttgag atgtcggccg cggagcagtt
     1681 gtgagacaaa ccatcaatcc cccgcctaat attctcttcg taaggcacgc atataaaatt
     1741 tatcatttcg atccgcgtgc ccgtcccgct ccccgagatc cttaaaaggg cggcgtctat
     1801 gccgtccata gaagtgccgg agataagtcc gacgacgagc ttttcttttt tatcgagcag
     1861 tgatctgagt ttttcgagca tgcggtaccg cgttccggtt taatcccggg accaattttt
     1921 attggcgccc cccggaggcc ggatcagctt aacgcttttc gtaagaagcc gttatttttg
     1981 aataataaat ccttcgcttt tttatagcct ataccctttt tcgccatcac gacggacgcc
     2041 ttcacgtccc agccggattt cttgagcagc cttccagcct cccgctcgtc cactcccgcg
     2101 atctctgtaa caattcgcac cgccctgtcc ctgagtttgg ccgagcgcgg caggacgtcg
     2161 accatgaggt tgccgtaggt ctttccgagc ttgaccatgg cggctgtcgt gagcatgttc
     2221 agaaccattt tcgtcgcggt gccggccttc atgcgagtgg ag
//
LOCUS       DASSJH010000122         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_460863, whole genome shotgun sequence.
ACCESSION   DASSJH010000122 DASSJH010000000
VERSION     DASSJH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460863"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..2477
                     /gene="pbpC"
                     /locus_tag="VFJ67_02670"
     CDS             102..2477
                     /gene="pbpC"
                     /locus_tag="VFJ67_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HET7289316.1"
                     /translation="MAGRRRFLKKLAVISLLSSSAFVLSALALFFALDLLFPFPSETL
                     RREPTAVISDKNGKPLRFFLPADKRWRFPVKLSDVSPELVRAVIASEDRWFHSHPGVN
                     PFALMRAFYTNIRERKVVSGASTIPMQIARMSDPKPRTIYSKAEEAFRALQLKRLFSD
                     DELLEIYLNLAPYGGNIEGVGAASYFYFGKGPRALTPGEIALLTALPRSPTAYDPVRN
                     PIDSERERNEVLAQLAERGVLTSEEAAEGRKEPVPRSRKPQPFSAPHFSEFVYREVSK
                     GGSLKTTLDPTIQRIAEEVAKRHIEILRNDGIDNLAIVVIENDTRKLRAMVGSADYFD
                     KDHSGQVNFTLARRSPGSALKPFLYAMALDEGIVVPDSYVLDIPTDFSGYVAENYDSK
                     YRGKITLENALILSLNAPAVRMLAKVGLKDFHALLLKGGLSTLDRPSEKYGLPLILGS
                     GEVRLLDLTNLYATLASGGKHEPFIVAEGGDHDDAVKELFSPEAAYLITEILTGLKRP
                     DMPANSWALTEDIPEVAWKTGTSYGHRDAWSLGFSDKYTIGVWVGNPDGRGQKGISGS
                     EHAAPILFDVFRSVEGDGSGITMPEGLRLGTIEVCGESHLLPTPYCGERDKITYIKGV
                     SRIPRDNYSKKIFVDSESGDLLLGDCIGERPHGSRVVMTYPPELVAWWMAEGKKPDTI
                     PPLSPYCNDVSSERPPEILSPDKSTPYRVLEGMPSEYQRVELIAGVSEEVTALYWYQD
                     GELVASTPPFKKTFIPLTAGTHRLVVVDSSGRSDSIRYKVESRDKIEVVAK"
     gene            2537..>2888
                     /locus_tag="VFJ67_02675"
     CDS             2537..>2888
                     /locus_tag="VFJ67_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289317.1"
                     /translation="MTGLFVCSFLLFCLPAASGSADNNPGLKFLASGKPVGEITLDVM
                     KSKLKTYRIELYDIEYKKEKRYEGFKVKDVLRLAYGDGLDDTDYTDIAFTALDGYESV
                     SLLPKMKEEGGYIVF"
BASE COUNT          655 a          796 c          842 g          595 t
ORIGIN      
        1 caggtacacc ggaaagctcg agaacccgtt cgtcgttaaa atgagacagt gatatttgtg
       61 tggacggaat gatggatagt ggtgaggaga tgcggagtac tatggccgga cgtaggcggt
      121 tcttaaaaaa actggcggta atttctttgc tgtcgtcgtc cgcgttcgtc ctgtcggcgc
      181 tggcgctttt tttcgcgctc gatctcctct tcccttttcc ttcggaaacg ctcaggagag
      241 agcccaccgc cgttatatcg gataagaacg gcaaaccgct caggttcttt ctcccagcgg
      301 acaagcggtg gcgcttcccg gtcaaactgt ccgatgtgtc ccccgagctc gtgcgcgcgg
      361 tcatagcttc cgaggacagg tggttccaca gccatccggg cgtgaatccg tttgcactga
      421 tgagggcttt ttacacgaat ataagagaga gaaaggtcgt atcgggcgcg tccacgatcc
      481 ctatgcagat agcccgtatg tccgatccca agccgaggac aatatattcg aaggctgaag
      541 aggcgtttag ggcgctgcag ttaaagcgtt tgttcagcga cgacgagctg ctcgaaatct
      601 atctcaatct cgcgccgtac ggtggtaata tagagggcgt aggcgcggcc tcttattttt
      661 atttcgggaa gggcccgcgc gctctaacgc cgggcgagat agcgctgctt acggctctcc
      721 cgcgctcgcc tacggcttac gaccccgtgc ggaacccgat agactcggag agggaaagga
      781 acgaggtcct ggcccagctc gcggaaagag gggtccttac atccgaggaa gcggccgagg
      841 gaaggaaaga gcccgtcccc cgctcgagga agccccagcc cttctcggcc ccgcacttct
      901 ccgagttcgt ttacagagaa gtctcaaagg gcggcagcct gaaaacgact ctcgacccca
      961 cgattcagag gatcgctgag gaggtggcga aacgtcacat tgaaatcctc cggaacgacg
     1021 gcatagacaa tctcgcgata gtagttatag aaaacgacac ccgaaaatta agagccatgg
     1081 tcggctccgc tgattacttc gacaaagacc attcgggcca ggtcaatttc actctcgcga
     1141 gacgctcgcc cggctcggca ctaaagccgt tcctttacgc tatggccttg gacgagggca
     1201 tcgtggtgcc tgacagctac gtgttagaca ttccgaccga cttctcgggc tatgtcgccg
     1261 aaaattacga cagcaagtac agggggaaga taaccctcga gaacgcgctt atactttcct
     1321 taaacgcccc cgccgtcagg atgctggcca aggtaggcct caaggatttc cacgcactgc
     1381 tgttaaaagg cgggctgtcc acgctcgaca ggccgtcaga aaaatacggg ctcccgctca
     1441 tactgggctc gggagaggtg aggctcctcg acctcactaa cctttacgcg actctcgctt
     1501 cgggcggaaa gcacgagccg tttatcgtcg ccgaaggcgg agatcatgac gacgctgtga
     1561 aagagctctt ttcgcccgag gcggcttatc tcatcactga aatactcacg gggctcaagc
     1621 gcccggatat gcctgccaac tcatgggctc tcactgagga cattcccgaa gtagcctgga
     1681 agacaggcac gtcctacggg cacagggacg cgtggtctct cggtttctcc gacaagtaca
     1741 cgataggcgt atgggtggga aatcccgacg gcaggggaca gaagggcatc tccggctcgg
     1801 agcacgcggc ccccatactg ttcgacgttt tcaggagcgt ggaaggggac ggctcgggga
     1861 taacgatgcc ggaggggctc aggctcggta cgatcgaagt gtgcggggag agccacctcc
     1921 tgcccacccc ctactgcgga gaaagggaca agataacgta tatcaagggc gtatcgagga
     1981 ttccgaggga caattactcg aagaagatat ttgtcgattc ggagtcgggg gacctcctcc
     2041 tcggggactg cataggagag cgtccccacg ggtcgcgcgt cgttatgacc tatccccccg
     2101 agctcgtcgc atggtggatg gccgaaggca agaagcccga cactatccct ccgctcagtc
     2161 cctattgcaa cgacgtctca tccgagcgtc cgcccgagat tctgtcgccc gataaatcca
     2221 cgccttaccg tgttctcgaa ggtatgcctt ccgagtatca gagggtggag ctcattgcgg
     2281 gagtgagcga ggaggtcaca gcgctctact ggtatcagga cggagagctc gttgcctcga
     2341 cgcccccatt caagaaaacc ttcatcccgc tcacggcggg cactcaccgt ctcgtggtcg
     2401 tggactcttc cgggcgctcc gacagcatca ggtataaggt cgagagcagg gacaagatag
     2461 aagtggtagc caagtgatgg cggctttaat cgcactgtta gctaggttgc gggcgggagc
     2521 ggggctttgt cattacatga ccggcctatt cgtttgttcg ttcttattat tctgcctccc
     2581 ggcggcttcc ggctccgccg ataataaccc cggcttgaaa ttcctcgcat ccggaaaacc
     2641 ggtcggcgaa ataacgctcg acgtcatgaa gtcgaagctg aagacttaca ggatagagct
     2701 ctacgatata gagtacaaaa aggaaaaaag gtatgaaggg tttaaggtca aggacgtgtt
     2761 aaggctcgcg tacggcgatg gattggacga taccgactac acggatatag cattcaccgc
     2821 acttgacggc tacgaaagcg tttccctcct cccgaagatg aaggaagaag gcgggtacat
     2881 cgttttca
//
LOCUS       DASSJH010000123         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_677714, whole genome shotgun sequence.
ACCESSION   DASSJH010000123 DASSJH010000000
VERSION     DASSJH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677714"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..866
                     /locus_tag="VFJ67_02680"
     CDS             12..866
                     /locus_tag="VFJ67_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006917634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7289318.1"
                     /translation="MSIVKRKKIFDNHTHIGPVPGFAYYGLPQAVKPTTDYDKTADYI
                     KGMDKHGVDRALVMSNYGYPDSAQPFTLNPLVTDSVQSSDRLLGAIWVSALPKDKERT
                     TEALKLIGEKGLVALKTTCLLGGTFNPEQWDKDSTELWEMILNAAAENDMPFHIHTSP
                     GGGSDIDGALALIRKYGKKVKIHVVHMGGGVSGHIKFVPRFFDLVEEGYQVYTDSSWA
                     VGFGSTWLLEEIEKRGVGGDRFLFGSDIPWSDFAGEYWKIEGADVSEKLKEDIFWNNA
                     EKLYGKFW"
     gene            996..1598
                     /locus_tag="VFJ67_02685"
     CDS             996..1598
                     /locus_tag="VFJ67_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET7289319.1"
                     /translation="MKPSPHTQFGLFSVRNFLSPIECGRIRSEMSSAEGNPASIVKTD
                     ERLLDENVRRTVRKEVSEETRRLVSEKLMGLRDELGAYFGVELSHPQKPEFLYYREGD
                     FFQCHTDKGMNPKNPQEVKDRLVSAVVFLNDEKDEPGPDTYTGGSFIIYGILNDPRFE
                     SQGFKVTGTAGTLIAFRSETFHEVTAVTSGMRYTAVSWFA"
     gene            complement(1914..>2106)
                     /locus_tag="VFJ67_02690"
     CDS             complement(1914..>2106)
                     /locus_tag="VFJ67_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289320.1"
                     /translation="SSLPVPPPQPIINNATTKIGIHTRSPIPFLPLANTPILLPLLFT
                     ERRGGPDDAVHPVQAGYRY"
BASE COUNT          504 a          563 c          620 g          419 t
ORIGIN      
        1 gaggcggtat catgagcata gtcaagagaa agaagatttt cgacaaccat acccacatcg
       61 ggcccgtgcc cggattcgct tactacggcc tgcctcaggc cgtgaagcct acgacggatt
      121 acgacaagac ggccgactat attaaaggta tggacaagca cggggtcgac cgcgccctcg
      181 tcatgtccaa ttacggctac ccggattcag ctcagccctt cactctcaac ccgctcgtca
      241 cggacagcgt gcagtcgtca gacaggcttt taggagcgat atgggtctcc gcacttccca
      301 aggacaagga gcgcacgacc gaggcgctca agctcatcgg cgagaaggga cttgtcgcgc
      361 tcaagaccac ctgtctcctc ggtggcacgt tcaaccccga gcagtgggac aaggattcga
      421 ccgagctctg ggagatgata cttaacgccg ctgccgagaa cgacatgccg ttccacatac
      481 acacgagccc gggcggaggc tccgacatag acggcgctct ggccctcatc agaaaatacg
      541 ggaagaaggt caagatacac gtcgtccaca tgggaggcgg agtgagcgga cacatcaaat
      601 tcgtcccgag gttcttcgat ctcgtcgaag agggttatca ggtatatacc gactcctcgt
      661 gggcggttgg gttcggctcc acgtggcttc tcgaagagat agagaagcgg ggcgtgggcg
      721 gagaccgttt cctcttcggc tcggacatac cgtggagcga tttcgcgggc gaatactgga
      781 agatagaagg cgccgacgtc tcggagaagc tgaaagagga tatattctgg aacaacgccg
      841 agaagctcta cggcaagttc tggtaaacgg gagaggcgcg cggaccggca tagcagaata
      901 ggagaccgga atagatacgg cctcaattga tacggaaata ctgattcgct agtattccga
      961 gagtcggatg tgttccgttt gatatcccga gacacatgaa accttcccca cacacgcaat
     1021 tcggtctctt ctcggtcagg aacttcctga gcccgatcga atgcgggcgt ataaggagcg
     1081 agatgtcttc cgccgaagga aatcccgcga gcatagtgaa aaccgacgag aggctcctcg
     1141 acgaaaatgt tcgccgcacg gtgaggaaag aggtatccga agagacgcgc aggctcgtaa
     1201 gcgaaaagct catggggctg agggacgagc tgggggcgta tttcggcgtc gagctctcgc
     1261 acccgcagaa gcccgagttc ctttactaca gagagggcga cttctttcag tgccacacgg
     1321 ataaagggat gaatcccaaa aatcctcagg aagtgaagga ccgcctcgtt tccgctgtcg
     1381 tgtttctgaa cgacgaaaag gacgagcccg gccccgatac ctatacgggg ggttctttca
     1441 tcatatacgg gatactgaac gacccgcgct tcgagtcaca agggtttaaa gtgaccggaa
     1501 cggcgggcac cctcatagcc ttccggtcgg agacgttcca cgaggtcacg gcggtcacga
     1561 gcggcatgag atacacggcc gtgagctggt tcgcgtgaag acgaaatacg cgcatattac
     1621 cgcatttcac agagctcccg gcacgcgggg gaactgccgg ggcttccccg ctggtcttat
     1681 agcgccttaa gactttttct ataccccgca cctttcatac atactcaggt attaccgatt
     1741 atttataccg tcgtccgatt gttgcggcgt ccccgcgcgg ttattctcaa aaccgagtta
     1801 aaaaaatccc ccgagagggt ggtctctcgg aggacttgac gccgacactg ttttggaaga
     1861 gccggcagtg agtcatgact cactgctaaa aataattaca gttcggaacc gtgtcaataa
     1921 cggtatccgg cctgtactgg atgcaccgcg tcgtccggtc ctccccgcct ctcggtgaag
     1981 agaaggggta agagtatggg ggtatttgcg agcggtagga aggggatcgg gctccgcgtg
     2041 tgtatcccga ttttagtggt cgcgttattt atgatcggct gtggaggcgg caccggaagc
     2101 gaagac
//
LOCUS       DASSJH010000124         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_244894, whole genome shotgun sequence.
ACCESSION   DASSJH010000124 DASSJH010000000
VERSION     DASSJH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244894"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFJ67_02695"
     CDS             complement(<1..406)
                     /locus_tag="VFJ67_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289321.1"
                     /translation="MLDKFINLAFMPVARVLQYDYVAKPMKPLGTNNTMKIARAYNSL
                     LYGKYYEYRKDRYLHHFDELIRENGEPVSGINEMKDGWALDTSGTLPHLKQLLNDAHE
                     IIKERGGVKRTDFGRPWFQELPIDDLFDKYPSL"
     gene            complement(455..799)
                     /locus_tag="VFJ67_02700"
     CDS             complement(455..799)
                     /locus_tag="VFJ67_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289322.1"
                     /translation="MGIYRIYKGDDDRSHIEGLSSDDPFWKSVKTTSTMFLKEFPPGT
                     FLDWHPAPRKQVVIILSGSLEHVFRDGSRRAFGPGDVRVLVDTTGEGHTTRVLGDEAV
                     LVAVVPLDNGDA"
     gene            complement(809..1696)
                     /locus_tag="VFJ67_02705"
     CDS             complement(809..1696)
                     /locus_tag="VFJ67_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HET7289323.1"
                     /translation="MKKLSIAYTPDSDDIFNFYAWEHGHVTLGARGFEPVFSRGHIIE
                     LNRAALEARYDVVAVSSVFYPNVADRYWILCVGNSVGRNFGPVLVSKRYKSIDELRGK
                     RVGVGGHPTTGSGLALMYCPGIELVEMPFDSIVDAIVRGEADAGVMIHEELIHYRERG
                     LECVCDLGAAWCEDTGLPLPVGLNLVRKDLGRELARDIAATCQSSLLWGFGHSSEVYE
                     YASKFGRGMAREHIEMFRIEDFLRLSEDVREAMKIMFARVASMGLGPRVDSFEVVEGL
                     DNPERLLVMRDKHDDLAVG"
     gene            complement(1729..2709)
                     /locus_tag="VFJ67_02710"
     CDS             complement(1729..2709)
                     /locus_tag="VFJ67_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289324.1"
                     /translation="MIGKAMDTLLKPAARALERNYMSALGVNNTMTLARAYNSLRYGS
                     NHEQRKRRYRDNFSELIAENGNPVAPVNEIKDGWVIDTSGRLPYLGELLEESSLIIDE
                     RGGEKHQFYRRPFFQDLFTDDYFRRCPSILNFATSSDVLTTVCNYLGFIPTLSYSKPY
                     GVRLIESWNKFDETPDAQPRESQLFHLDYHDKPMVYVIVVLREVTMERGPFCFLPKSV
                     SAKAETGLGDYHTKKGGHRVKDERMYSVVPENELIKLCYPAGTVLILDSSACFHYGSR
                     NAVKPRHLMMFAYVSTIRTDFGDIVLRNMRYPVRKEDSRLRRMLLDKEYI"
     gene            2966..3286
                     /locus_tag="VFJ67_02715"
     CDS             2966..3286
                     /locus_tag="VFJ67_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289325.1"
                     /translation="MMFFMMLAALYITCVNAYAQSFQEYNDAGSGGYDSPAVRERHTG
                     PPREAIETCEGLGESEACAFTSPRGDEIAGTCEYVPGDKFACVPEGGPAGERNPPDYV
                     PPAE"
BASE COUNT          780 a          995 c          866 g          810 t
ORIGIN      
        1 gaagagacgg atacttgtcg aacaagtcgt ctataggcag ctcctgaaac cacggccgtc
       61 cgaaatcggt gcgctttacg cctccccgct cctttataat ctcatgggcg tcgttcaata
      121 gctgcttcag gtgggggagt gtcccggacg tgtcgagcgc ccagccgtct ttcatttcgt
      181 taattccgga tacgggctct ccgttctcgc ggatcagctc atcgaaatga tggagatacc
      241 gatccttcct gtattcataa tatttgccat ataaaagact gttataagcc cgcgcgatct
      301 tcattgtatt atttgtgccg agcggcttca tgggttttgc aacataatcg tattgcagca
      361 ccctcgctac gggcataaaa gccaggttta taaatttatc caacatgcga tacccctcct
      421 cgggcttggg tcgtgctccg gtctctcatc tactttaagc atccccgttg tcaagcggca
      481 ccacggcgac gagcacagct tcatcgccca ggacccgggt cgtatgaccc tcgcccgtcg
      541 tatccacgag caccctcacg tctcccggtc cgaacgcgcg cctgctgccg tcgcggaaca
      601 cgtgctccag agaccccgac aatataatta ctacctgctt ccttggagcc gggtgccaat
      661 cgaggaacgt gccgggcggg aactctttta aaaacatcgt cgaagtagtt ttgaccgatt
      721 tccagaacgg atcgtccgat gaaagtcctt cgatatgaga tctatcgtca tcgcccttat
      781 aaatcctgta tatgcccatg cgtcgagttc acccaactgc caggtcatca tgtttgtccc
      841 gcattacaag gagccgttcg ggattatcca agccttcgac cacctcgaaa gaatcgaccc
      901 tagggccaag gcccatcgac gcgactcgtg cgaacattat tttcatcgcc tcgcgcacgt
      961 cctcggaaag cctgaggaaa tcctcgatcc tgaacatctc gatatgttcc ctggccatgc
     1021 ccctgccgaa tttactcgcg tactcataga cctcggaaga gtgcccgaat ccccagagga
     1081 gcgacgactg gcaggtcgcg gctatatcac gcgcaagctc cctgccgagg tccttccgca
     1141 cgaggttaag acccacaggc agggggagcc cggtgtcctc gcaccatgcg gcaccaaggt
     1201 cgcacacgca ttcgagtccc cgttcacggt aatggatgag ctcctcgtgg atcatgacac
     1261 ccgcgtccgc ttcgccgcgg acaatcgcgt ccactatcga atcaaacggc atctccacga
     1321 gctcgattcc cgggcagtac ataagcgcaa ggccgctgcc cgtcgtcgga tgccccccta
     1381 ccccgacccg ctttccgcgg agctcgtcta tcgatttgta ccgcttcgag acgagcacgg
     1441 gaccgaaatt cctgcccacg ctgtttccca cgcatagtat ccaatacctg tcggcgacat
     1501 tcggatagaa aacggacgac acggccacga catcgtatct tgcctcaagc gccgcccggt
     1561 taagctcgat gatgtggcct ctcgaaaaaa cgggctcgaa gccccgagcg ccgagcgtta
     1621 cgtgtccgtg ctcccaggcg tagaaattga agatatcgtc gctgtcggga gtgtaggcaa
     1681 tagatagctt cttcacaatg attccttata gtctctcgaa atcgtcattt agatatattc
     1741 tttatccagc aacatacgcc tcagccgtga atcctccttc ctgacggggt atctcatgtt
     1801 cctgagcact atatcgccga aatccgtcct gatcgtggag acgtacgcga acatcatcag
     1861 atgacgcggc ttcactgcgt tgcggctgcc gtagtgaaaa cacgcgctgc tgtcgaggat
     1921 caacacagtc cccgccggat agcacaactt tatcagctcg ttttcgggga cgacggaata
     1981 catcctttcg tccttgacgc ggtggccgcc tttctttgta tgataatcgc cgagaccggt
     2041 ttcggccttc gccgacacgg acttgggcag gaaacaaaaa ggtcctctct ccatagtgac
     2101 ctctctcagg acgactatca catagaccat cggcttatcg tgatagtcga gatgaaagag
     2161 ctggctctcc ctgggctgag cgtccggagt ttcgtcgaac ttgttccagg actctatgag
     2221 cctcacgccg tagggtttgg aatatgaaag agtgggtatg aagccaaggt aattgcagac
     2281 ggtggtaagt acgtccgagg aagtggcgaa attgagtatc gaagggcatc tccggaaata
     2341 atcgtcggta aataaatcct ggaaaaaagg tctcctgtaa aattgatgct tttcgccgcc
     2401 ccgctcgtct attatcaggc tcgactcctc gagaagctcc ccgagataag gcagcctccc
     2461 cgaggtatcg atgacccacc cgtcttttat ttcgttcacg ggcgcgaccg gattaccgtt
     2521 ttcggctatc agctctgaga agttatcccg ataccgtctc tttctctgct catggttact
     2581 tccgtacctg agactgttgt aggccctggc gagcgtcatt gtattattca ctcccagggc
     2641 gctcatgtaa tttctctcga gggccctcgc ggccggtttt aatagtgtgt ccatagcttt
     2701 accgatcatg cagcctcccg agtggattaa aggtccacat tgaatcagcc gatatatatc
     2761 cggcccgctc cgtaggatcg accccccatc gctcgcgcgg ttcctagtat attgtatgaa
     2821 attcgcaaag aaatcaatcc gtgaaaccgg cggtgataat tcgccgcctt cagacaccca
     2881 taccgaatga tttgagcgcc tctcaacgta taaacaataa aaagcggggg tagaaatggg
     2941 aattaatcgg tcagcattcg gaaaaatgat gttttttatg atgctcgccg ctttatacat
     3001 aacatgcgtc aatgcgtatg cgcagtcttt tcaggaatat aacgacgcgg ggagcggcgg
     3061 ctatgattcc ccggctgttc gggaaagaca tacgggccct ccccgtgagg caatagaaac
     3121 gtgcgaaggc ctgggagagt ccgaggcctg cgctttcact tcgccccggg gagacgagat
     3181 cgcgggcaca tgcgagtacg tgcccggaga taagttcgcc tgcgttcctg aaggcggccc
     3241 tgccggagag agaaatcccc ccgattatgt cccgcccgct gagtaagctt tactaccgcc
     3301 gggcggacgc acaaggggtt aattacgttc gcggagaaaa tcgagtgaat cacgaaaggc
     3361 acaaatcaga catatcgtgg gtccggttca ttgcggcgct tcttctcatt ccggttttcg
     3421 taataaccgg aatactattc accatagtcg a
//
LOCUS       DASSJH010000125         4598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1434710, whole genome shotgun sequence.
ACCESSION   DASSJH010000125 DASSJH010000000
VERSION     DASSJH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434710"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..504)
                     /gene="ssrA"
                     /locus_tag="VFJ67_02720"
     tmRNA           complement(151..504)
                     /gene="ssrA"
                     /locus_tag="VFJ67_02720"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(639..1088)
                     /gene="smpB"
                     /locus_tag="VFJ67_02725"
     CDS             complement(639..1088)
                     /gene="smpB"
                     /locus_tag="VFJ67_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET7289326.1"
                     /translation="MEKVVCTHPRAKRDYDIEENYEAGIELKGSEVKSLRGNQASIKE
                     SFAMIRDEEVFLVNSYIAPYEQASVFNHDPRRERKLLLNKREISRLMGKTIIRGYTLI
                     PLKIYFKRGKAKVDLALGKGKKVHDRRDDIKKREADREMEKAMKRRY"
     gene            complement(1499..1948)
                     /gene="dtd"
                     /locus_tag="VFJ67_02730"
     CDS             complement(1499..1948)
                     /gene="dtd"
                     /locus_tag="VFJ67_02730"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET7289327.1"
                     /translation="MRAIVQRVKVARVEVDGETVGRIGEGMLVLLGAGKDDTEEDAEY
                     LAEKILTLRIFEDAEGKMNLSVMDTGGGVLVVSQFTLYGDCRKGRRPSFDKAAPPETA
                     ERLYVLFVAELRERGAKVETGRFRAMMDVHLVNWGPVTLMLDSKREF"
     gene            2089..2430
                     /locus_tag="VFJ67_02735"
     CDS             2089..2430
                     /locus_tag="VFJ67_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7289328.1"
                     /translation="MRILNVIPVLMIVLGISLRAVNSIEFPPEPPKKLIIHTVKSGEE
                     LHLLAGYYFLDCRQWPQIYQANMGTIVNPNKIYPGQQLYVYVDNDWTPPFDLDAYVEK
                     WRPYLLSGTVP"
     gene            complement(2427..3626)
                     /locus_tag="VFJ67_02740"
     CDS             complement(2427..3626)
                     /locus_tag="VFJ67_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7289329.1"
                     /translation="MKKQKDLEKITPRLAEIESGAPDENYGMYSFGGDDGFDPEDAEF
                     DPEFASREGKSGGGKKNRKSKLEEHLRLLHVYFKDLEHETSLLSPQEELRIAANIKKC
                     ENKAEAIRLVLSEISRETSERRSGLKKYPAYAGKNLINYMDRMNTLSNAYVKRSSELK
                     NRFIKSNLKLVISIAKNYMGRGLPLADLIQEGNLGLMRAVEKFDYTLGYKFSTYASWW
                     IQQAVFRAPFEKTKTIRIPVYLYEWAGKVRAVTTMLSKELGREPTCEEIAKELDISPS
                     VVKRIIQTASDVMNNVQSLDTPVSNEDQRTHVEFVEDDKIPPPDSAILKRTVQNRIED
                     ILSLLTPKEQEIIRMRFGIGIDTTYTLNEIGDRFGVTRERIRQIEKSALDKISSSDVG
                     EYLRDLI"
     gene            complement(3851..4270)
                     /locus_tag="VFJ67_02745"
     CDS             complement(3851..4270)
                     /locus_tag="VFJ67_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289330.1"
                     /translation="MATSKMAYDIKDFTEPVKELSKLVKETYLNTLEFSLSVAEENKS
                     VIDKQLEYVIDAEKEYFNSIKDFYGTLPASDYPFGKVDTRAFEDGLTKVFEFQKNLVD
                     SFKGISGSVAADSHDFAKKNVIKAFTMFDEALDTIKV"
BASE COUNT          995 a         1217 c         1107 g         1279 t
ORIGIN      
        1 taatcggctt accatggcgt atagtccggc gctgttcaca atacactgag ccatagcccg
       61 gcttcgcttt agccgattct cacctgtctt tactgtgaaa gttaaggtag tttcctgccg
      121 taagccaccc gaagctcgaa gagcgaaggg tggtggaggc gccgggaatc gaacccgggt
      181 ccgagaaaga gactcacaag gcctctacat gcttagtcta tcattagttc tcgcagtgaa
      241 gttaccgata gacagggttt tcaccgccag ccccgaatgt tttctcgtcc tcaggcgttc
      301 agggcgtcgc catcggacca gcccgcattg tgtcgcctcg tttagacccg cgggcggggt
      361 ctaaggagcc gtcgccgctc tatttaggca gcgagtgcaa agtcatttgt ggcacttgtg
      421 tgtgctccgc cagttttacg agttagcaga agctcggcat gcagcacaag ctatcccaat
      481 ccccgtcgaa gccatgtcgc ccccattgtc aaagaacaaa atacttgaat aatgctattt
      541 aacttgccat gataaaataa ttattgtttt gccgatgtca agcccgccgc ccctgatccg
      601 gttgatatag tttaccttat tttcgggaat gtacggagtc agtagcgccg cttcatggcc
      661 ttctccatct ccctgtcggc ctctctcttc tttatgtcgt cccgcctgtc gtgcaccttt
      721 ttaccctttc cgagcgcgag atcgaccttg gccttccccc tcttgaagta tatcttgagc
      781 gggatgagcg tgtatcccct tatgatggtc ttacccatga gccggctgat ctcccgcttg
      841 ttgagcagga gcttcctttc cctgcggggg tcgtggttga atacgctcgc ctgctcgtaa
      901 ggggcgatat agctgttgac gagaaagacc tcctcgtccc ttatcatggc gaagctctcc
      961 ttgatgctcg cctggttccc gcggagcgat ttcacctcgg agcccttgag ctctattccg
     1021 gcctcgtagt tctcctctat gtcgtaatcc ctctttgccc tcggatgagt gcagaccacc
     1081 ttttccattt ttgtaatata atccctgaac agtggaaatc aacacgcaca atgtgtgctc
     1141 atttatgatc ctactgtatc tcttccgctc aacccagcac gtaccccctc ccgtttgtcc
     1201 taagctcgtc gaagggttga acgggtatta ctactgtagg agcggcttcc agccgcgatt
     1261 ccataatccc ttccccctag aggggggaag gttaggatgg gggtgttacc actcattcac
     1321 acaatccatc atggtgaact cgtttcagca tctcatcatt tatcttttag ttccctcctt
     1381 agaaaaagga ggacaattcc gagcatgtga ggaattggtc ggtgcagata aaaggtgaag
     1441 caatgtctta gtcgaagtaa tgcaggtagt aaatccaaat aagggaggat ttaattattt
     1501 aaaattccct cttactgtcg agcatcagcg tcacggggcc ccagttcacg aggtgcacgt
     1561 ccatcatcgc gcggaacctg cctgtctcta ccttcgctcc gcgctccctc agctcagcga
     1621 cgaaaagcac gtagagcctc tccgcagtct cgggaggggc ggccttgtcg aacgacggac
     1681 gcctgccctt ccggcagtcg ccgtagagcg tgaactggga gacgaccagc accccaccgc
     1741 ctgtgtccat cacggacagg ttcatcttgc cttccgcgtc ctcgaatatg cgaagtgtca
     1801 atattttctc cgcgagatac tcggcgtcct cctccgtatc gtccttcccg gcccccagga
     1861 gcacgagcat accctcccct atccggccca cggtttcgcc gtccacctcc accctcgcca
     1921 cttttacacg ctggactatg gctcgcatga agcgcttccc gtatcgacgg tctttaagtg
     1981 ttgtcctaac atcccgaaga taatacctgt ttggaaaatg attaacatat gaggaatatg
     2041 tattataatt gagatatata gggtcttata tcaaaggggc gggttactat gcgaatactt
     2101 aatgtgattc cggtattgat gatagtcctg ggtatttcac tgcgggcggt gaattcgatc
     2161 gaattccctc ccgagccgcc gaagaagctc attatccaca cggtcaagag tggggaggag
     2221 ctacatctat tagcgggtta ctatttcctc gactgcaggc aatggcctca gatataccag
     2281 gcgaacatgg gcactatcgt gaatccgaac aagatatatc ccggacagca gctttacgtc
     2341 tatgtggata acgactggac gccgcccttc gatttggacg cctacgtcga gaaatggcgg
     2401 ccttacctct tgagcggcac tgtgccttaa atcaggtctc ttaaatattc cccgacgtcg
     2461 gaagacgata tcttgtcgag cgcggatttc tcaatttgcc ttatcctctc gcgcgttacc
     2521 ccgaatctgt cccctatctc gttcagcgta taggtggtgt caatcccgat gccgaagcgc
     2581 atccttatga tctcctgctc cttaggcgtg aggagagaga gtatgtcctc gatcctgttc
     2641 tggacagtgc gcttgagaat ggccgagtcg ggaggaggaa tcttatcgtc ctcgacgaac
     2701 tctacgtgtg tcctctggtc ttcgttcgag acgggggtgt cgagggactg cacgttgttc
     2761 attacgtctg acgcggtctg gattatcctc ttgacgacgg agggggatat atcgagctcc
     2821 tttgctatct cctcgcacgt gggctcgcgc ccgagctcct tcgacagcat ggtcgttacg
     2881 gccctcacct tgcccgccca ttcgtagaga tatactggta tcctgatggt cttcgtcttc
     2941 tcgaagggtg ccctgaagac ggcctgctgt atccaccagc tggcgtaggt cgagaacttg
     3001 taaccgaggg tgtagtcgaa tttctcgacc gcgcgcatca gcccgaggtt cccttcctga
     3061 atgaggtccg cgagggggag tcccctcccc atgtagttct tggcgatgct tatgacgagc
     3121 ttgaggttgg acttgatgaa cctgttcttt agctcggagg acctcttcac gtaagcgttg
     3181 gataaggtat tcatcctgtc catgtagttg atgaggtttt tgcccgcgta agccgggtat
     3241 tttttaaggc cgctccttct ctcgctcgtt tctcttgaaa tctccgaaag cacaagtctg
     3301 atggcttcgg ccttgttctc gcacttcttt atgttggcgg cgatcctgag ctcttcctgg
     3361 ggcgaaagga gagaggtttc atgctcgagg tccttgaagt aaacgtggag gagcctgagg
     3421 tgttcttcga gtttggactt ccggttcttc ttcccgccgc cgcttttccc ttcccgggag
     3481 gcgaattcgg gatcgaattc cgcgtcctcc gggtcaaaac catcatcccc gccgaatgag
     3541 tacatcccgt agttttcgtc aggcgcgccg gattcgatct cggcaagcct cggagttatc
     3601 ttttcgaggt ctttctgctt tttcatattt cccctgatcc tatcccctat acacagggga
     3661 ttcctctata aagtcaaaac tatacgcacc ttcgataccc ttgcggatat gagggtaccc
     3721 gggttcaccg gagcttattc gaaccgttat gtgatatcga ataactgttt aaaacggttg
     3781 atttaggtta ttcgggagag ccgggataat tggccctccc gtttaatttt ttattttcaa
     3841 taaaacccga tcaaaccttt atggtatcga gcgcctcatc gaacatcgtg aaagccttta
     3901 tcacgttctt cttcgcgaaa tcgtggctgt cagcggccac actgcccgaa atacccttaa
     3961 atgagtctac caagtttttc tggaactcaa aaacctttgt gagtccgtcc tcaaaagctc
     4021 tcgtgtcaac tttgccgaac ggataatcag aagccggcag cgtgccgtag aagtccttta
     4081 tcgagttgaa atattctttc tcggcatcta taacatattc gagctgcttg tctatcacgc
     4141 tcttgttttc ctcggctacg gacaaggaga attcgagcgt attgagataa gtttccttca
     4201 cgagcttcga taattccttc accggctccg tgaagtcctt tatatcataa gccattttac
     4261 tggtcgccat agtaagccct cctgtttatg gtctaaaaac aatataatga gttggaatat
     4321 atttgtcaag agaaaatgta cgcaattgcg aagatttata aattcgtatg tgactgtgat
     4381 tattgattat acataatccg accagaaata atattatgca ttagcttata ttttgaagct
     4441 ttgcgatacg gattgcgcta tcttaaaaaa tgggaaattg gaagacctat agccgggatg
     4501 ataaagctat acggatggcc taggggcagt tcgattactt gaggtgtgct atataaccta
     4561 ctgttatata tacagatata ctattctgag ctcttctt
//
LOCUS       DASSJH010000126         3421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_136516, whole genome shotgun sequence.
ACCESSION   DASSJH010000126 DASSJH010000000
VERSION     DASSJH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136516"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VFJ67_02750"
     CDS             <1..1237
                     /locus_tag="VFJ67_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET7289331.1"
                     /translation="ELDYVLKDSGAEFVIAAPGFGERLSRLADSRSARYIGLGDALSA
                     RSAPLPELRGEKKAMIIYTSGTTSRPKGVVTTHDNIRAQIGSLVTAWEWSRSDFILDV
                     LPLHHLHGILNVLLCALYSGARCEMLSGFDSGRVWKRFIERDYTLFMAVPTIYSRLIR
                     SFEEAGDAEKETMSRAYRKMRLMVSGSAALPVNTLEKWREISGHVLLERYGMTEIGMA
                     LSNPLRGERRPGRVGMPLPGVDVRLADESGKVIEGEGAGEIFVKGKNVFIEYWNNPEA
                     TREAFTEGGWFRTGDIAERDAGGSCRILGRSSVDIIKSGGYKISALEIEEVLREHPHI
                     EECAVVGIGDEEWGERVCAAVVMPGGENTTPESLREWLKDRLAPYKIPSRIIMVGELP
                     RNPMGKVIKPEVIALFRDI"
     gene            1350..2072
                     /locus_tag="VFJ67_02755"
     CDS             1350..2072
                     /locus_tag="VFJ67_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289332.1"
                     /translation="MNIKLLSSLVLALIVISFPVWAQGQSMGSKSGNISSQQTGQSKL
                     GAGKQTRSNNTPGMNVNSNPGRNQGFGNGNNPDKNNRHDSGDNNKGDWHGINDKWDGK
                     HHHDRKHHKFNPLFAPYYYFGYYGYAPYTGYYAYVDPDYPLGYGTTMGTSLERPSNLE
                     INQFNEDAPAPQNYRQDNSGAGYIYTEPQEDTVVEYYNPPPSEEQTIYVWVDEGGVQN
                     YANDIDLVPLRYRDIATVLGAE"
     gene            2617..>3421
                     /gene="gcvT"
                     /locus_tag="VFJ67_02760"
     CDS             2617..>3421
                     /gene="gcvT"
                     /locus_tag="VFJ67_02760"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET7289333.1"
                     /translation="MFPIHRQLGAKIVEFAGWEMPVQYEGLREEHLAVRRDAGLFDVS
                     HMGEIEIRGKEAERFCQYITTNDVRAVKEFQAQYTLFCNPEGGVVDDVILYKFSRKKY
                     LFCVNASNTQKDYEWIIRANDNFDIEIEDRSAQYSQIALQGPRSEDILSACLSADFSA
                     LRRFHFLPFTWKGMELIIARTGYTGEDGFEIFLPWDGGPDLWMSLSESGRDCGIRPCG
                     LGARDTLRIEMGYSLYGHEISDDINPFEAGLGRYVKTDAGDFIGRDALCG"
BASE COUNT          873 a          821 c          970 g          757 t
ORIGIN      
        1 cgagctcgat tacgtgctca aagactcggg cgcggaattc gtgattgcgg cgcccggatt
       61 cggcgagagg ctctcccggt tggcggattc aaggagcgca agatatattg gcctcgggga
      121 cgctctctcc gcccggagcg cgcccctgcc cgagttgagg ggggaaaaga aggcgatgat
      181 aatttacacg agcggcacga cgagcaggcc caagggcgtc gtcacgaccc acgacaacat
      241 ccgcgcccag atcggttccc tcgtcacggc atgggaatgg tcccggagcg atttcattct
      301 cgacgtgctg ccgctccatc acctccacgg catactgaac gtgctcctct gcgcgcttta
      361 ctcgggcgcg cgctgcgaga tgttgagcgg tttcgattca ggccgcgtgt ggaagagatt
      421 catcgagcgt gactatacgc ttttcatggc cgtgcctacg atatattcga ggctcatccg
      481 ctccttcgaa gaagccgggg acgcggagaa ggaaacgatg agccgcgcat accgcaagat
      541 gcgcctcatg gtgtcgggct cggcggccct gcctgtaaac acgcttgaga aatggagaga
      601 gattagcggt cacgtgcttc tcgagcggta cggcatgacg gagataggca tggctctttc
      661 gaaccctctc cgcggggaaa gacgcccggg ccgggtcggc atgccgctcc ccggagtcga
      721 tgtgaggctc gcggacgaga gcgggaaagt gatcgagggt gagggagcgg gtgagatatt
      781 tgttaaagga aaaaatgttt tcatcgaata ctggaacaac cccgaagcga cgagagaggc
      841 ttttaccgag gggggttggt tcaggacggg cgacatcgca gaaagggacg ccggagggag
      901 ctgcaggata ctcgggagga gctccgtcga tatcatcaag agcggcggat acaagatttc
      961 ggcgctcgag atagaggagg tcctgaggga acatcctcat atagaggaat gcgccgtcgt
     1021 cggtatcgga gacgaagaat ggggagagag ggtttgcgcg gccgtcgtta tgcccggcgg
     1081 cgaaaacaca accccggaat cgctaaggga atggctgaag gacagactcg ccccctacaa
     1141 aataccgtcc cgtatcatta tggttggaga gctcccgcgt aacccgatgg ggaaggtcat
     1201 caagcccgaa gtcattgcat tattcagaga tatttaaacc gcaaacccgc aatgacccac
     1261 atgatttgct aaatgaaaga tatattaaat agagaaccga ttctgtataa tatacgtata
     1321 gtttccagag ggaagaacgg ggtgatttaa tgaatattaa acttttgtcc tcactggttc
     1381 tggcgttgat cgtgatttcg tttccggtat gggctcaggg gcagagtatg ggctccaaaa
     1441 gcggaaacat aagtagtcaa cagacggggc agtcaaagct tggcgccggt aagcagacga
     1501 ggtcaaataa tacgcccgga atgaacgtta actcaaatcc cggtcggaat cagggattcg
     1561 gcaacggcaa caaccctgac aaaaacaacc gtcacgattc cggcgataat aataagggag
     1621 attggcacgg cattaatgac aagtgggatg gtaaacatca tcacgacagg aaacatcata
     1681 aatttaatcc tctctttgcg ccctactact atttcggcta ctacggctat gcgccttata
     1741 ccggttatta cgcgtatgta gaccccgact atccgctcgg ttacgggact acgatgggca
     1801 catctctcga aaggccgagc aatctggaga tcaaccaatt caacgaagac gctccggctc
     1861 ctcagaacta ccgtcaggat aattccggtg ccgggtatat atataccgag ccgcaggagg
     1921 ataccgttgt cgagtactac aatccccctc cttccgaaga gcagactata tacgtatggg
     1981 tcgacgaggg cggcgttcaa aattatgcga atgacatcga tctcgtgccg ctcaggtaca
     2041 gggatatagc aacagtcctg ggcgccgaat gaccgcgtct gcaaaataaa cccgcaatcc
     2101 ttacatattc tcgtcacggc gcagaacctc cgctcgcaca cgtagtgtgt ttttatgatt
     2161 ccttccaact cctccgctcg aaccggcaat tcgccaatat tcctgtcatt gctgtagatg
     2221 ttaaataatt cggggctgga attcttctga atggcacgga atgcaggggg gatttatctt
     2281 ttccgacatt acagaatttg cgttaagaaa atcgcgtgtt taagcgtaaa aaaatctttc
     2341 agctttctat tgaaagattt tagattaaac gcaagatttt tagcaaatta tgtagtcgga
     2401 tgatttttgc ttcttttcat caaggaaaag aagaaaaaca tatttttgcc ttctttatgt
     2461 attccaggcc cttaagaaaa ctcagtgcta acgatggtca ggccgaatta cttgacacat
     2521 gcagtaaagc ggtctcccga atatttacgc ttccgtatta ttgacaaccc cattttgagt
     2581 tgttaaacaa ttagagtgaa atcgaagaaa acggcgatgt ttcccatcca ccggcagctg
     2641 ggagcgaaga ttgtcgagtt tgccggatgg gagatgcccg ttcagtacga gggtctcagg
     2701 gaggagcatc tcgcagtcag acgtgatgcg ggtctgttcg acgtgagcca catgggagag
     2761 atagagataa ggggaaagga agccgagcgg ttctgtcagt atataacgac gaacgacgtc
     2821 cgggcagtaa aggaatttca ggcccagtac actctcttct gtaaccccga gggcggggtc
     2881 gtcgacgacg tcatcttata taaattttct cggaagaagt atttgttctg cgtaaacgcg
     2941 tcgaatacgc agaaagacta cgagtggatc atcagggcca acgataattt cgacatcgag
     3001 atcgaagaca ggagcgccca atactcacag atcgcgctgc agggcccgcg ctccgaggac
     3061 attctatcag cctgtctgtc tgcggatttc agcgctttga ggaggttcca ttttcttccg
     3121 tttacgtgga aagggatgga gcttataata gcgaggaccg gttacacggg cgaggacggg
     3181 ttcgagatat tcctgccctg ggacggcggt cccgatctct ggatgagcct gtccgaatcc
     3241 gggcgcgatt gcggaatcag accgtgcggt ctcggagcgc gcgacactct caggattgag
     3301 atgggttatt cgctatacgg gcacgagata agtgacgaca taaacccttt tgaagcgggc
     3361 ctcgggaggt atgtgaaaac ggacgccggg gattttatcg gcagagacgc cttgtgcgga
     3421 g
//
LOCUS       DASSJH010000127         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_461516, whole genome shotgun sequence.
ACCESSION   DASSJH010000127 DASSJH010000000
VERSION     DASSJH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461516"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFJ67_02765"
     CDS             <1..270
                     /locus_tag="VFJ67_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289334.1"
                     /translation="NDGAVLVLFNLKKNYRFSELIYEAGDEKKLKCPLCSSRKVEKVV
                     SSFAFTSDLSTNMPKPDLGNLPPSVRNRTFLGEYIEEKDRPKKNR"
     gene            563..2299
                     /locus_tag="VFJ67_02770"
     CDS             563..2299
                     /locus_tag="VFJ67_02770"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HET7289335.1"
                     /translation="MKFLNQQILYIQLLRKPCALFISLVLLACFLAAGCDEGENVFSG
                     GGEGPDNDPPGAVSGPSVVFSQNAPLAAVLSLTSDEPTEVEVDVTGSAAEANSVSPRA
                     EDFTVHSDGFDTVHAITLLGFHPDSPYNIHVTLRDESGNETELNDVGVVTDPLPVGFP
                     PIHVETSMPELMEPGVTLFPVRASGANLAFGSVFVALDESGQVVWYRRFANIGYGDIR
                     HISNGNFLFIEDDATITEMNVLGDIVRRWHTNRNTNPSAGSILVDIPIFHHEVFEMEN
                     GNFLVLTVGLRSIENYPTSDSDPEAPLETAVVAGDEVVEFNPVDGTIINHWVLLDMLD
                     PFRIGYDSLGGFWNNNFPEIEAGTRDWAHANAVIHDPSDDSIIVSMRHQDAVVKFSRQ
                     TGQLIWILGTHANWDPVEFGSFLLDPIGDPFLFQYHQHAPETTPAGTILLYDNGNFKA
                     SPFETKLTAPENFSRAVEFSINKDTKQVTQVWEFGEFDDPIRYAPFIGDADMQPITGN
                     VLITHGGVTNDAEGLPSDNIGLSKTSVHIIEVTHTTPGEKVFEISIVDPTPEIANGWT
                     TYRSERLPSLYP"
     gene            complement(2315..>2538)
                     /gene="hisE"
                     /locus_tag="VFJ67_02775"
     CDS             complement(2315..>2538)
                     /gene="hisE"
                     /locus_tag="VFJ67_02775"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HET7289336.1"
                     /translation="KGLDKILKKIGEEAGETIIGAKNGDRDEVVYETADLWFHSLIAL
                     SYFGITPGDIYRELGKRFGKPSESYRNKS"
BASE COUNT          609 a          671 c          657 g          601 t
ORIGIN      
        1 aacgacggcg ctgtactcgt cctttttaat ctgaaaaaga attaccgttt ctcggagctc
       61 atatacgaag ccggagacga gaaaaagctt aaatgcccgc tctgctcgag cagaaaagtc
      121 gaaaaggtgg tatcgagctt cgcctttacg agcgaccttt cgacgaatat gcccaaaccc
      181 gacctcggta atctcccccc ttccgtaagg aacaggacgt tcctcggcga gtatatcgag
      241 gaaaaggaca ggcccaagaa aaaccgctga ccatccgacg attaataagc tgccggctta
      301 gcgggaaatc cctgccgtcc gtatttccgg ctatataatg acttttgtcc gggatgatct
      361 gtaacgcgct gtgtttattg catattgcag gactgcctgg ctgaaactct aatagcgatc
      421 caagactctt tatgcatacg gcccggagag gtcaaattgt tttgtgaaat tgaaatcctg
      481 tattgaatca cggttataat tattgtaata atagttaaag gcattacacc cgttttataa
      541 tttactagga tcggaggatt tcatgaaatt tctgaatcaa caaatattgt acattcaact
      601 attgcgcaaa ccgtgcgctc tcttcatttc tctcgtactg ctcgcgtgct tcctcgcggc
      661 gggatgcgat gaaggcgaaa atgtgttctc gggaggaggg gaagggcccg ataacgatcc
      721 gcctggggct gtgtccgggc cgtctgtcgt cttttcccag aacgcccctc ttgctgccgt
      781 cttgagtctc acgagcgacg agccgaccga ggtcgaagtc gatgtgacgg gcagcgccgc
      841 ggaagcgaat tccgtttctc cgcgtgccga agattttacc gtccatagcg acggtttcga
      901 taccgtgcac gcgataacgc tcctcgggtt tcacccggac agtccctata acattcatgt
      961 aacgttgagg gacgagtcgg gtaacgagac tgaattgaac gacgtcggcg ttgtcactga
     1021 cccgctcccg gtgggctttc cgcctataca tgtcgaaacg agcatgccgg agctcatgga
     1081 gcccggagtg actctcttcc ccgtaagggc gtcgggagcg aatctcgcct tcggcagcgt
     1141 cttcgttgca ttggacgagt cgggtcaggt cgtctggtac aggaggttcg cgaacatagg
     1201 ctacggcgac atcagacata tctccaacgg gaatttcctc ttcatcgaag acgatgctac
     1261 gatcaccgaa atgaacgtgc tcggagacat agtacgtaga tggcacacca atcgtaatac
     1321 caatccgagt gcgggcagca tacttgtcga tattccgata ttccaccacg aagtcttcga
     1381 gatggagaac ggcaattttc tcgtgctgac cgtcgggctg aggtcaattg agaactatcc
     1441 gaccagcgat tcggatcctg aagcgccgct cgagacggcc gtagtcgcgg gcgacgaggt
     1501 ggttgagttt aaccctgttg acggcacgat aataaaccac tgggtgctcc tagatatgct
     1561 cgatcccttc aggatcggat acgattccct tggcggattc tggaacaaca atttccccga
     1621 aatcgaggcc gggacaaggg actgggccca cgcgaacgcc gtaatacatg acccttccga
     1681 cgattccata attgtttcaa tgaggcatca ggacgccgtt gtgaaattca gccgtcagac
     1741 gggccagctc atatggatac tcgggaccca cgccaactgg gacccggttg agttcgggag
     1801 cttcttgctc gaccctatcg gggatccttt cctgttccag tatcaccagc acgcccctga
     1861 aacgactcca gcgggaacca tacttcttta cgacaacggc aactttaagg cgagcccgtt
     1921 cgaaacgaaa ttaacggccc ccgagaattt cagccgtgcg gtcgagttct cgataaataa
     1981 agatacgaaa caggtcacac aagtttggga attcggagag ttcgacgacc caatacgtta
     2041 cgccccgttc ataggcgacg ccgatatgca gcccatcacc gggaacgtgc tgataacgca
     2101 cggaggagtc acgaacgatg cggaaggcct gccgtctgac aatataggct tatcgaaaac
     2161 atcggttcac ataatagagg tgacgcatac tacgcctggg gaaaaggtgt tcgagatttc
     2221 tatagtcgat ccgacgcccg agattgcaaa tggatggact acgtacagat ccgagaggct
     2281 cccgagtctc tatccctgag acagggcggg ttgattaact cttgtttcgg tatgactcgc
     2341 tgggcttccc gaacctcttg cctagttcgc ggtaaatatc ccccggggtt ataccgaaat
     2401 aagaaagcgc gatgagcgag tgaaaccaga ggtcggccgt ttcgtaaacg acttcgtccc
     2461 tgtctccgtt cttggcccct attatcgttt ctcccgcctc ctctccgatc tttttcagta
     2521 tcttgtcgag tccttttt
//
LOCUS       DASSJH010000128         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_245395, whole genome shotgun sequence.
ACCESSION   DASSJH010000128 DASSJH010000000
VERSION     DASSJH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245395"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFJ67_02780"
     CDS             <1..297
                     /locus_tag="VFJ67_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289337.1"
                     /translation="WKRVENIDFYSVTVKGYKFRKTAGCVYAGQYAVYKGPFKFVIDE
                     EGHVFPRSQAVEVCTDTAAKLSAAPYIGMFMITEYETKPEGAACCSPKGGTGCC"
     gene            332..667
                     /locus_tag="VFJ67_02785"
     CDS             332..667
                     /locus_tag="VFJ67_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HET7289338.1"
                     /translation="MDYYDPKDLKRFSEIGKFGEELMKKLFDYYNAATGEDGALTKRE
                     KALIALAVAHVKQCPYCIDAYTTQCLETGADPEQMTEALHVAASLEAGITLIHGIQMH
                     NVLEKNGAL"
     gene            732..1769
                     /gene="arsS"
                     /locus_tag="VFJ67_02790"
     CDS             732..1769
                     /gene="arsS"
                     /locus_tag="VFJ67_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644845.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HET7289339.1"
                     /translation="MPQAQALRQDSDRSDVSDEIKYDFNGTLRENGQELRPVSIDTLQ
                     INVTRLCNQACVHCHVDASPRRREQMDLSTVNRCLEILEDHDRIRNLDITGGAPELNP
                     HFEHLVREAVKLGKHVMVRHNLTVTLDGNPVTGESKSHLPAFYAGLGVEVISSLPYYQ
                     EYFTDKQRGKGVFEKSIKSIILLNSVGYGMENTGLVLNFVYNPVGAFLPAPQKSLEAD
                     FKRELRRKYGIVFNNLYTITNMPINRFKQQLKKIGAYDEYMTRLVNAYNPAAAEGVMC
                     RSLVSVGYDGRIYDCDFNQMLDLALTGKGKPMTVFDFDYERLLKRRIIFGSHCFGCTA
                     GSGSGCGGATA"
     gene            1821..2456
                     /locus_tag="VFJ67_02795"
     CDS             1821..2456
                     /locus_tag="VFJ67_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET7289340.1"
                     /translation="MNILIIFVKYPDPGKVKTRIARELGAEKAADLYSLIAGSVIENV
                     SGSDKYRTVFFFDPPEMENGIRKWLGREDATYEPQSGNTIGERMSDAFERVFSGGAEK
                     AVLIGTDVPEITGETVNRAFRLLEFEDAVIGPAEDGGYYLLGLKKPEPLLFDDIEWGS
                     DSVYKRTVERIEKLDLSYKPLDTLRDVDTAEDVGPELLKWLTGDSKLRNSN"
     gene            2601..3128
                     /locus_tag="VFJ67_02800"
     CDS             2601..3128
                     /locus_tag="VFJ67_02800"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7289341.1"
                     /translation="MKRGFLLLSPVLLLFLFLVISCDQGNGEPGKESDQNPAGDFSLE
                     PVDGGGKISLRDFKGKPVVLNFWATWCGPCKEELPIFQKMWDKFKDEDVVFLGVDVMD
                     DRSNASEFIKNSGISYTMLYDQAGDVSSKYKVIALPATFFINKEGEIEVKNYGPFIGK
                     DGEKKFKLYMKEITE"
     gene            3125..3880
                     /locus_tag="VFJ67_02805"
     CDS             3125..3880
                     /locus_tag="VFJ67_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET7289342.1"
                     /translation="MNPADVQVSWVIAFLAGIISFLSPCVLPLIPGYVSMVSKMSFEE
                     LTENTTEGKTGRILVPSLLFVLGFSTVFVTLGASASFMGTFLHENKVLLLRISGVIII
                     LFGLFSMDILKIPQLYRERRLYFTGGNLGFIGVFFLGIAFGFGWTPCVGPILASILLY
                     ASTSEGAGKGAALLFTYSVGLGLPFIITGLALSKALTAFGWIKRHYGLYKVIVGGTLI
                     VVGVLMVTNNLYYLNIYGQRALDWAGIDFWKSF"
     gene            3921..>4197
                     /locus_tag="VFJ67_02810"
     CDS             3921..>4197
                     /locus_tag="VFJ67_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289343.1"
                     /translation="MNPQTALQEKIRDIQCLSEGDVHVWEIALDDPALKTGGPFGDIL
                     SEDEKTRASRLIPGVLRDRFVTARVYMRIILGRYLDMPPGGIGFEYNK"
BASE COUNT         1079 a         1041 c         1190 g          887 t
ORIGIN      
        1 tggaagaggg tcgagaatat cgatttctac tcggtgaccg tgaagggcta caaattccgg
       61 aagactgccg ggtgcgttta cgcagggcag tatgcagttt acaagggtcc gttcaagttc
      121 gtgatagacg aagagggaca cgtctttccg aggagccagg cggtcgaggt ctgcacggac
      181 accgcggcaa agctctcggc ggctccgtac atcggaatgt tcatgatcac ggaatacgag
      241 acgaagcccg agggcgcagc gtgctgctct cccaaaggcg ggacgggctg ctgctgagaa
      301 tgatacttgg aaataaaatg ggaggtgccg gatggattac tatgacccga aggacctgaa
      361 gcggttttcc gagataggaa aattcggcga agagctgatg aagaaattat tcgactatta
      421 caacgcagcc acgggagagg acggcgcgct cacgaaaagg gagaaggccc tcatagcgct
      481 tgccgtcgcg cacgtgaagc agtgtccata ctgcatcgac gcttacacga cacagtgcct
      541 cgagaccggc gccgacccgg agcagatgac cgaggcttta cacgttgccg cttcgcttga
      601 ggcgggcata acgcttatac acggtatcca gatgcataat gtcctggaaa agaacggcgc
      661 attgtaattt tccatatata cggatacaag cctgaggatt caggatcgaa ggagcataac
      721 gggagcaagg tatgccgcaa gcacaggcgc taaggcaaga tagcgataga tcggacgtga
      781 gcgacgagat caaatacgat ttcaacggaa ccctcaggga gaacggacag gagttaagac
      841 ctgtctcgat agatacgctc cagataaacg tcacgaggct ctgcaaccag gcctgcgtgc
      901 actgtcatgt tgacgcttcc cccagaagaa gagagcagat ggatctcagt accgtgaacc
      961 gctgccttga gatactcgaa gaccacgatc gtataaggaa tctcgacatc acgggcggag
     1021 cgcccgagct caatccccac ttcgagcacc tcgtgagaga ggccgttaag ctgggaaagc
     1081 acgttatggt gagacacaac cttacggtca ccctggacgg gaatccagtg accggggaga
     1141 gcaaatccca cctgccggct ttctacgccg ggctcggagt ggaagtcatc tcctcgctcc
     1201 cctattacca ggaatacttc acggacaagc agagggggaa gggcgtattc gaaaaaagca
     1261 tcaaatcaat aatactcctc aactccgtcg ggtacggcat ggaaaataca gggctcgtgc
     1321 tcaatttcgt ctataacccc gtgggagcgt tccttccagc gccccagaag agcctcgaag
     1381 ccgatttcaa gagagagctt cgcagaaagt acggcatagt tttcaataac ctctacacga
     1441 taacgaacat gcctatcaac cggttcaagc agcagctaaa aaaaatcggg gcctacgacg
     1501 agtacatgac aaggctcgta aacgcgtaca acccggcggc ggcggaaggc gtcatgtgca
     1561 ggtctctcgt gagcgtaggt tatgacggca ggatctacga ctgcgatttc aaccagatgc
     1621 tcgatctcgc gctcacaggg aaaggaaagc cgatgacggt cttcgatttc gattacgagc
     1681 gcctcctcaa gagaaggatc attttcggct cacactgttt cgggtgcacg gcgggctctg
     1741 ggagcggctg cggtggcgct acggcataaa acgaaggacg cctaacgcgt atcagaactc
     1801 atttaccggg gagcgcgcga gtgaacatcc tcataatttt cgtcaagtac ccggatccgg
     1861 gaaaagtcaa aacgaggatc gccagggagc tcggagcgga gaaggcagct gacttatatt
     1921 cgctcatagc ggggagcgtg atagaaaatg tatcgggttc ggataaatac cgaaccgtat
     1981 tctttttcga cccgcctgaa atggagaacg gaatcaggaa gtggctcggg cgggaagacg
     2041 caacatacga gccgcagtcg ggaaatacta tcggagaaag gatgtccgat gcattcgaaa
     2101 gggtattttc aggtggggcg gaaaaagctg tcctgatagg gacggacgtt cctgaaataa
     2161 caggagagac ggttaatcga gccttccggc tcctcgaatt tgaggacgcc gtgatagggc
     2221 ccgctgaaga cggcgggtat tacctccttg ggctcaaaaa accggagccc ctcttgtttg
     2281 atgatatcga gtggggctct gacagtgtgt ataagcgtac agtcgaacgc atcgagaagc
     2341 tcgatctcag ttataaaccc ctcgatacat tgagagatgt cgacacggcg gaagacgtcg
     2401 ggcccgagct cctaaaatgg cttacgggtg attcgaaatt acgaaattcg aattaaacgt
     2461 caatatgagt ccggccccgc aattcccggc cctgaatccg atccgtctgt cgctccgtat
     2521 tctattaaat cgacacataa aaaatatgaa tggtatatta tttagcactt cctatacgaa
     2581 tgccgcacga ggagataaat atgaagagag gtttcctgct tctttcaccc gttctgcttt
     2641 tgtttctgtt cctagtaata tcgtgtgacc aggggaacgg ggaaccggga aaggaatcgg
     2701 atcagaaccc tgcaggggac ttttctctcg agcccgtgga cggaggcggc aagatcagcc
     2761 tcagggactt caagggtaag cccgtagtgc tcaatttctg ggccacgtgg tgcgggccgt
     2821 gcaaagagga gcttcctatc ttccaaaaga tgtgggacaa gttcaaggac gaggacgtcg
     2881 tattcctggg cgtggacgtt atggacgaca gatcaaacgc ttccgaattt ataaaaaatt
     2941 cgggaatctc ctataccatg ctatacgacc aggcgggcga cgtgtcttca aaatacaagg
     3001 tcatagccct cccggccaca tttttcataa acaaggaagg tgaaatagag gtcaagaatt
     3061 acgggccgtt tatcggaaaa gacggagaga agaaatttaa actctacatg aaggagataa
     3121 ctgagtgaat ccggccgatg tgcaggtaag ctgggtcatc gcgttcctgg cgggtatcat
     3181 ctcgttcctc tctccgtgcg tactcccgct tataccggga tacgtatcga tggtttccaa
     3241 gatgtcgttc gaggagctga cggagaatac gacagagggg aaaacgggaa ggattctcgt
     3301 cccgagcctc ctcttcgttc tgggtttctc gactgtattc gtcacgctcg gagcgtcggc
     3361 ttcatttatg ggcacgttcc tccacgagaa caaggtgctc ctcctcagga tctcgggcgt
     3421 cataataata ttgttcgggc tcttctcgat ggacatactc aagattcccc agctctacag
     3481 ggagcggcgg ctctacttta caggcggcaa cctcggcttc atcggcgtat tcttcctcgg
     3541 catcgcgttc gggttcggat ggacgccgtg cgtaggtccc atactggcct cgatactact
     3601 ctacgcgagc acatccgagg gagccggaaa gggcgcggcg cttcttttca catattcggt
     3661 cgggctcggc ctgcctttca tcatcacggg gcttgcgcta tcgaaggcgc tcacggcttt
     3721 cggatggata aagcgccact acggcctcta taaagtcatc gtcggcggga cgctgatcgt
     3781 cgtgggcgtg ctcatggtca cgaacaacct ctattatctg aacatatacg gccagagggc
     3841 tcttgactgg gcgggaatcg atttctggaa atcgttctga acagttgaga aaatcaggcc
     3901 gaaacctgta tcatatatat gtgaatccgc aaactgcgct tcaggaaaaa atccgcgata
     3961 tccagtgcct gagcgagggc gacgtccacg tatgggagat agcactcgat gacccggctc
     4021 tcaaaacagg gggcccgttc ggtgatattt tgtcggagga cgagaaaaca agggcatcga
     4081 ggctcattcc cggcgttctc agggacagat tcgtcacggc gcgcgtttac atgaggatta
     4141 tactgggcag gtatcttgac atgccgcccg gcgggatcgg gttcgaatac aataaac
//
LOCUS       DASSJH010000129         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_353690, whole genome shotgun sequence.
ACCESSION   DASSJH010000129 DASSJH010000000
VERSION     DASSJH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353690"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VFJ67_02815"
     CDS             <1..1198
                     /locus_tag="VFJ67_02815"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET7289344.1"
                     /translation="KAREQMLVTRAKDYSAVVAYCNLVGGQDELVFDGHSVVISERGE
                     ILARAKGFQEDLLVSDINIDRVFRSRTHDPRRRKEQLAMKAHSASAEVIVIESGKDTR
                     KKKPAVKSGVEEFLSVDEEIFRALVLGTRDYVTKNGFRKVVVGLSGGIDSAMVTVIAA
                     EALGGENVVAVTMPSRYSSRGSVGDSEKLAKNLGIELLNIPMEKAFKAYGDMLSGVFA
                     GLKEDTAEENLQARIRGNILMALSNKFGWLVLTTGNKSEMSVGYCTIYGDMAGGFAVI
                     KDVPKTIVYRAARFYNAWKKKETIPAAILTKPPSAELKPGQLDTDTLPPYDVLDPILK
                     AYVEDDLSVEDIISLGFDERIVKRVARMVDTNEYKRRQAPPGIKITARAFGKDRRFPI
                     TNLYRE"
     gene            1380..>3682
                     /locus_tag="VFJ67_02820"
     CDS             1380..>3682
                     /locus_tag="VFJ67_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HET7289345.1"
                     /translation="MKPVGYPKKQGLYDPQFEHDSCGIGFVVNIKGKKSHAIIANALT
                     VLRNLQHRGACGCEANTGDGAGILIQTPHKFFKKVAGEAGITLPPYQSYASGAVFLAP
                     NASDRKECIKAFERIVGEEGLSVLGWRDIPTDNSSLGFTAVKGEPYMKQVFIARGKSI
                     PDDAAFERRLYIIRKRAENEIRFSGMQGGEYFYIPSLSWKTFVYKGMLTTWQLELYYP
                     DLSDPDMESAIALVHSRFSTNTFPSWDRAHPYRYIIHNGEINTLRGNINWMHAREAVL
                     SSDLFGDELEKLFPIIQPDGSDSANFDNCVEFLTLAGRPIEHSIMMMIPEPWSNHETM
                     SDEKKAFYEYHSCLMEPWDGPASIAFTDGFKVGAVLDRNGLRPSRYYVTKDDMVIMGS
                     EVGVLDVPADMVLRKGRLQPGRMFLIDTIEGRIVDDEEIKNKLANEKPYREWLNENLV
                     NLSDIPLSEGVKDSEEYDHDAMIVRQRVFGYTFEDLRIIMGPMAENGVEPVGSMGKDT
                     PVAVLSNKPRLLFEYFKQLFAQVTNPPIDAIREELITAAGVTIGPEKNLLDPRPENCR
                     LIKLDSPILTNEQLERIKGLDDGTFKSVTLPILFEVSGGSDSLEDALKDLFTEADKAI
                     EEGANLIILSDRGFDREHAPIPSLLASSGLHHHLIRNGKRLKVALILESGEPREVHHF
                     ALLIGYGVSAVNPYLAYESIEDIIEEGLLRNIDKKTAIKNYIKSLVKGVVKTMSKIGI
                     SAVQSYHGAQIFEAIGLNHEFVDKYFT"
BASE COUNT          906 a         1037 c         1050 g          689 t
ORIGIN      
        1 aaaggcgcgc gagcagatgc tcgttacgag ggccaaggac tactcggccg tcgtcgccta
       61 ctgcaacctc gtcggggggc aggacgagct cgtgttcgac ggccacagcg tggtaataag
      121 cgagcgcggc gagatactcg cccgggcgaa ggggtttcag gaagacctcc tcgtttcgga
      181 cataaatata gaccgcgttt tcagatcgcg cactcacgac ccgaggagaa ggaaagagca
      241 gctggcgatg aaagcccatt cggcgagcgc cgaggtcata gtaatagaaa gcgggaagga
      301 cacccggaag aagaaacccg ccgtgaagtc gggcgttgaa gagttccttt ccgtcgacga
      361 ggaaattttc agggcgctcg tgctcgggac cagggactac gtgacaaaga acggtttcag
      421 gaaggtcgtc gtcggcctga gcggcgggat agattccgcc atggtgacgg tgattgcggc
      481 cgaagccctc ggcggggaaa acgtcgtcgc ggtaaccatg ccctccagat acagctcccg
      541 gggcagcgtc ggggactccg aaaaacttgc gaaaaatctg ggtatagagc tcctcaatat
      601 accgatggaa aaggcattca aggcgtacgg cgatatgctg tcaggggtgt tcgccggatt
      661 gaaagaagac actgccgaag agaacctcca ggcccgcatc cgtggcaaca tactcatggc
      721 gctttcgaac aagttcggat ggctcgtcct cacgaccggc aataagagcg agatgagcgt
      781 cggctactgt accatatacg gagacatggc cgggggcttc gccgtcataa aggacgtacc
      841 caagacgatt gtgtacaggg cggcccgctt ttacaatgcc tggaaaaaga aagagaccat
      901 acccgccgcg atcctgacca aacccccatc ggccgagctt aagccgggcc agctggacac
      961 ggacacgctt ccgccctatg atgtgctcga cccgatactc aaggcctacg tcgaggacga
     1021 cctcagcgtg gaagacataa tatccctcgg gttcgacgaa cggatcgtga agagggtcgc
     1081 acgcatggtg gacacgaacg aatacaagcg caggcaggcc cctccgggca tcaagataac
     1141 cgcgagggct ttcggcaagg accggaggtt cccgattacg aacctttacc gtgagtgact
     1201 ccccccggat gatcttcgga cccgctgtcc gcactctatt gccctgggcc gtatcctgcg
     1261 gcccccttgt tgacttgtcc taattttcat ctaagattat tttagggtta atcggctgaa
     1321 atcagtgggc caattctttt tagttacata tcatctagag cttacaggaa ggtgtcttta
     1381 tgaagcctgt gggatatccg aagaaacagg gtttgtatga cccccaattc gagcacgatt
     1441 cgtgcggtat agggtttgtt gtaaacatta aaggtaaaaa atcccatgcc atcatagcga
     1501 acgcgctcac cgttctccgg aacctccagc acagaggggc ctgcggatgc gaagccaaca
     1561 ccggcgacgg cgccgggata ctgatccaga ccccgcacaa gttctttaag aaggtcgcgg
     1621 gcgaggccgg gataaccctc ccgccttatc agtcctacgc ttcgggcgcc gtattcctcg
     1681 cacccaacgc gtccgacagg aaggagtgca taaaggcgtt cgagaggata gtaggcgaag
     1741 aaggcctctc cgtactaggc tggcgggaca ttccgaccga caattcctcc ctcggtttca
     1801 cggccgtcaa gggcgagccc tacatgaagc aggtcttcat cgcgcgcggc aagagcatcc
     1861 ctgacgacgc cgctttcgag cggaggctct acatcatcag gaagcgcgcg gagaacgaga
     1921 taagattctc cggcatgcag gggggcgagt atttctacat cccgagcctc tcatggaaga
     1981 cattcgttta caaggggatg ctcacgacgt ggcagcttga gctttactat ccggatctct
     2041 ccgaccccga catggagtcg gccatagcgc tcgtccactc ccggttcagc acgaacacgt
     2101 tcccgagctg ggaccgagcg cacccatacc gctacatcat acataacgga gaaataaaca
     2161 ccctcagggg taacataaac tggatgcacg cccgcgaggc cgtactctcc tcggacttgt
     2221 tcggagacga gctggaaaag ttattcccca tcatacagcc cgacgggagc gattccgcca
     2281 atttcgacaa ctgcgtcgag ttcctcacac tcgccggacg gcccatcgag cattcgatca
     2341 tgatgatgat ccccgagccg tggtctaacc acgagacgat gagcgacgag aaaaaggcat
     2401 tttacgaata ccacagctgt ctgatggagc cgtgggacgg gcccgcgtcg atagccttca
     2461 cagacggctt taaagtaggt gcggtgctcg acaggaacgg cctcaggcct tcccgttatt
     2521 acgtaaccaa ggacgacatg gtgatcatgg gctccgaggt cggcgttctg gacgtccccg
     2581 ccgatatggt gctgcggaag gggaggctcc agcccggaag gatgttcctc atcgacacga
     2641 tcgaaggacg catcgtggac gacgaggaga ttaagaacaa gctagctaac gagaaacctt
     2701 atcgggaatg gctgaacgag aatctcgtca acctctcgga cattcccctt tccgagggcg
     2761 taaaggactc ggaggaatac gatcacgatg cgatgatcgt caggcaaagg gtgttcggat
     2821 atacgttcga ggacttgagg attataatgg ggcccatggc cgagaacggc gtcgagcccg
     2881 tgggctcgat gggtaaggat acccctgtag ccgtgctctc caacaagccg aggctccttt
     2941 tcgaatattt caagcagctc ttcgcccagg taaccaaccc tccgatagac gcgatacgag
     3001 aggagctcat cacggctgcc ggagtgacca taggtcccga gaaaaattta ctcgacccgc
     3061 gccccgagaa ctgccgcctt ataaagctcg acagccctat actcacgaac gagcagctcg
     3121 agcgcataaa ggggctcgac gacggcacgt tcaagtccgt cactctcccc atactcttcg
     3181 aggtttcagg gggaagcgac agcctggagg acgccttgaa agacttgttc accgaagcgg
     3241 acaaagcgat cgaggaaggg gcaaatctca tcatactgtc cgaccggggc ttcgacaggg
     3301 agcacgcgcc gataccgtcg ctgctcgcct cttcgggtct ccaccaccac ctcatcagga
     3361 acgggaagag gctcaaggtc gcgcttatac tcgaatcggg cgagcctagg gaagtccatc
     3421 atttcgcgct cctgataggc tacggcgtaa gcgccgtcaa cccatacctc gcctacgaat
     3481 ccatagagga catcatagaa gaggggctgc tcaggaacat cgataaaaag acagcgataa
     3541 agaactacat caagagcctc gtcaagggcg tcgtgaagac gatgtccaag atcggcatat
     3601 cggccgttca gagttatcac ggcgcccaga tattcgaggc cataggcctc aaccacgagt
     3661 tcgtggacaa atacttcacc tg
//
LOCUS       DASSJH010000130         4415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1216807, whole genome shotgun sequence.
ACCESSION   DASSJH010000130 DASSJH010000000
VERSION     DASSJH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216807"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /gene="alaS"
                     /locus_tag="VFJ67_02825"
     CDS             complement(<1..1538)
                     /gene="alaS"
                     /locus_tag="VFJ67_02825"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET7289346.1"
                     /translation="MTGSEIRKRYLEYFESKGHTIVKSSSLIPRNDPTLLFTNAGMVQ
                     FKDVFLGLDKRDYKRATSSQKCVRAGGKHNDLENVGYTSRHHTFFEMLGNFSFGDYFK
                     KEAIEFAWELITGVFKLPEDRLWVTVFTEDDEAADLWRKVVGLPPDRIVRMGEKDNFW
                     AMGDTGPCGPCSEIIIDQGKEHGCGRPECAVGCDCDRFLELWNLVFMQYDRDKTGKLN
                     PLPNPSIDTGMGLERITAVLQGVKSNYDTDLLRGIISNVEEISGKRYGDDPAADVSMR
                     VMADHARAVTFLISDGVFPSNEGRGYVLRRILRRAVRHAKKLGIEEPCLYRITGRVRD
                     IMRDAYPELDERIGFVAEVVRNEEERFFETIDRGLDLLNAEIEKLGGEKVIHGDTVFK
                     LYDTFGFPVDLTEDIARERGLTLDYAGFEKEMTKQREKSRKAWKGGEEEGLEPLYKDF
                     VSGGLTVAFTGYGKTRDTGRITAIIKDGVLADSAGEGDKVDIIIDKTPFYGESGGQVG
                     DKGT"
     gene            complement(1569..2630)
                     /gene="rlmN"
                     /locus_tag="VFJ67_02830"
     CDS             complement(1569..2630)
                     /gene="rlmN"
                     /locus_tag="VFJ67_02830"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7289347.1"
                     /translation="MNIKDLSPVELEKFLTDKGLERFRARQVGKWIYRKGARSFDEMT
                     DLSKDTRELLKSSFYLGDSMEIAEEQESADGTRKFLFRLLDGKQIESVLIPEENRNTL
                     CISSQVGCALGCTFCLTGTIGRIRNLTAAEILDQYMIVNEHSGGSVTNIVFMGMGEPL
                     DNLENLVRALKTMIDENFLGLPPKRITVSTSGIVPRIKELGEQVSVNLAVSLNAPRDE
                     LRDVIMPINKRYPIRELIKASVKFPVPPRKALMFEYVLLSKVNDSDRDAYDLGYLLKG
                     IKCKINLIPFNEAGPLPYRQPTRERVLRFQEILVSFGINVRIRKSKGRDILGACGQLA
                     AAYPARKIKDAKRVAGALI"
     gene            complement(2776..3774)
                     /locus_tag="VFJ67_02835"
     CDS             complement(2776..3774)
                     /locus_tag="VFJ67_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET7289348.1"
                     /translation="MDHAIIFVSLIVAVAPVMAVLFFIWWVDRYDREPLGYVFAAFLW
                     GGLGAIALSILGTEAGIRMLGNVVNYSGFDFPAVVIAPFIEEFMKGLVVLFLLRYRQF
                     DNVTDGLVYGAASGLGFGMTENFMYFTQFAGVSPGYEWLNLLFIRSMMTANMHCAASA
                     TFGAGISKLKDGGKSAWISVGIFYLLAVLLHGTWNFLVTSREEAYWGLAVIILIAYII
                     FIFVIFVRGIVEERRQRQVVLVEEFDLGVLPSDHRQILVSYMAMRRGRWFPDYLNKDK
                     YLALVGRLAMRKASYYKSEGKRQETLQKEIVEIRRELKRFSDLFQELRQEKPMEEA"
     gene            4023..>4415
                     /locus_tag="VFJ67_02840"
     CDS             4023..>4415
                     /locus_tag="VFJ67_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET7289349.1"
                     /translation="MSDLKTALDHGLTKEEYRKITDTLGREPNITELGILAAMWSEHC
                     SYKSSRIHLRKFPTTGEAVIQGPGENAGIIDIGDGQCAVFKMESHNHPSFLEPYQGAA
                     TGVGGILRDIFTMGARPIAVLDSLRFGDP"
BASE COUNT          958 a         1305 c         1081 g         1071 t
ORIGIN      
        1 atcgtccctt tgtcgccgac ctggccgccg gactcaccgt agaaaggtgt cttgtcgatg
       61 attatatcga ccttatcacc ctcgcccgcg ctgtcggcca agacgccgtc ctttattatc
      121 gcggttattc ttccggtatc gcgcgtcttg ccgtatcccg tgaacgcgac cgtaagcccg
      181 cccgagacaa aatctttata tagaggctct aacccctctt cttccccgcc cttccaggcc
      241 ttcctcgact tttccctctg tttcgtcatc tccttttcga acccggcgta atcgagcgtg
      301 agcccgcgct cgcgggctat atcctcggtg aggtcgacgg ggaatccgaa cgtatcgtag
      361 agcttgaaga ccgtgtcgcc gtgtatgacc ttctctccgc caagcttctc gatctcggcg
      421 ttaaggagat cgagccccct gtctatggtt tcgaagaacc tctcctcctc gttcctgacc
      481 acctccgcaa cgaaaccgat cctctcgtca agctccgggt atgcgtcccg cattatgtcc
      541 cttaccctgc ccgtgatcct gtaaaggcac ggctcttcga tcccgagctt cttcgcgtgg
      601 cgtacggccc ttctcaatat gcgcctcagc acgtagcccc ttccttcatt agacgggaac
      661 acgccgtccg atataaggaa ggtaacggcg cgggcgtggt cggccatcac cctcatagat
      721 acgtcggccg cggggtcgtc cccgtaacgc tttccggata tttcctccac gttcgagata
      781 atccccctca ggaggtcggt atcgtagttg ctcttcaccc cctggagcac ggccgtgatc
      841 ctctcgagcc ccataccggt gtcaatgctc ggattgggga gcggattgag cttcccggtt
      901 ttgtccctgt cgtactgcat gaagacgagg ttccagagct cgaggaacct gtcgcaatcg
      961 caccccacgg cgcattcggg tctcccgcac ccgtgctctt ttccctggtc gatgattatc
     1021 tccgagcacg gaccgcacgg gccggtatcc cccattgccc agaagttatc cttctccccc
     1081 atcctcacga tcctgtcggg cggcagtccc acgaccttcc gccagaggtc agcggcctcg
     1141 tcgtcttccg taaagaccgt gacccagagc ctgtcctcgg ggagcttgaa aacgcccgtg
     1201 atcagctccc aggcgaactc tatggcttcc ttcttgaaat agtccccgaa cgagaaattg
     1261 ccgagcatct cgaaaaacgt atgatgcctc gacgtgtaac cgacgttctc gaggtcgtta
     1321 tgcttccctc ccgctctgac gcacttctgg gatgatgtgg ccctcttgta gtccctctta
     1381 tcgagtccga ggaacacgtc cttgaactgg accattccgg cattagtgaa gaggagcgta
     1441 gggtcgttcc tggggatgag cgaagagctc ttcacgatag tgtggccctt gctctcgaaa
     1501 tattcgagat acctctttct tatttcagat cctgtcattt aaccgatttt cccgtactcg
     1561 tttttgtctc agatgagagc cccggcgact ctcttcgcgt cttttatttt ccttgcgggg
     1621 taagcggccg ccagctgacc gcacgcgccg agaatatctc gccccttgct cttccttata
     1681 cggacattga tcccgaacga cacgagtatc tcctgaaaac gcagcaccct ctccctcgta
     1741 ggctggcggt aaggcagagg gcccgcctcg ttgaagggta tgaggtttat cttgcatttg
     1801 atacctttca ggaggtatcc cagatcgtat gcgtccctgt cggaatcgtt gaccttgctg
     1861 aggaggacgt attcgaacat gagcgctttt ctgggcggga cggggaattt cacgctcgcc
     1921 ttgatgagct cccttatcgg atacctttta tttataggca tgatcacgtc tctcaattca
     1981 tctcgcggcg cgttgagcga tacggccagg ttgaccgaga cctgctcgcc cagctctttt
     2041 atcctgggaa ctatgccaga ggtcgaaacg gttattctct tcggcgggag gccgaggaaa
     2101 ttctcgtcaa tcatcgtctt gagcgccctt accaggtttt caaggttgtc gagcggctcc
     2161 cccatgccca tgaatacgat gttcgttaca gagccgccgg agtgttcgtt aacgatcata
     2221 tactgatcca gtatctcggc ggcggtcagg ttccttatcc tgccgatcgt ccccgtgaga
     2281 cagaatgtgc agccgagcgc gcaccccacc tgcgaggata tacaaagcgt attcctgttc
     2341 tcttccggaa taagcacgct ctcgatctgc ttgccgtcga ggagcctgaa gaggaacttc
     2401 cttgtgccgt cggcagattc ctgctcttcc gctatctcca tcgaatcccc gagataaaaa
     2461 gagcttttca gcagctctct cgtgtccttg gacaggtctg tcatctcatc gaacgaccgg
     2521 gcgcctttcc tgtatatcca ctttcccacc tggcgggcgc ggaaccgttc taacccctta
     2581 tccgtaagga atttttccaa ttctacggga ctcaaatcct tgatgttcat attttctttg
     2641 tatcgttaaa cccgtgtata tattatccaa gtaaggatac aggggggaga aaatttatac
     2701 aatagcggtc tgactcacag tggagtggtg tttgaatcaa atcccgtata acccggccca
     2761 atgctctata gccgatcatg cttcctccat aggcttttcc tgcctgagtt cctgaaacag
     2821 gtcggaaaac ctcttcagct ccctcctgat ctcgactatt tctttttgaa gagtctcctg
     2881 tcgcttccct tcagacttgt aatatgaagc ttttctcatt gccagccgcc ctacgagtgc
     2941 aagatacttg tccttgttga gatagtccgg gaaccatctc cctcttctca tggccatata
     3001 cgatacgagt atttgcctgt ggtcggatgg cagaacgccc aaatcgaact cctccacgag
     3061 cacgacctgc cgctgcctcc tctcttcaac gatacccctt acgaaaatta cgaatataaa
     3121 gattatgtaa gcgatgagta tgatgacggc caaaccccag taggcttctt cccttgaggt
     3181 gacgaggaag ttccaggtac cgtgcaggag tactgcgagc agatagaaga ttccgaccga
     3241 aatccaggcg ctcttgcctc cgtccttcaa tttgcttatg ccggctccga acgtggcgct
     3301 cgcggcgcag tgcatgttcg ccgtcatcat gctcctgatg aaaagaaggt tcagccactc
     3361 gtatcccggg gaaacgccgg cgaactgggt gaaatacatg aaattctcgg tcatcccgaa
     3421 cccgagaccc gaggccgctc cgtaaacgag cccgtcggtc acgttgtcga actgacggta
     3481 cctgagcagg aacaatacga cgaggccctt catgaattcc tctatgaagg gggcgatgac
     3541 aacggccgga aaatcgaatc cgctgtaatt gactacattg cccagcatcc tgatgccggc
     3601 ttccgtacct agtatggata gcgctatcgc gccgagaccg ccccagagga aagccgcgaa
     3661 tacataacca agaggctccc tgtcgtagcg gtccacccac cagataaaaa agagaaccgc
     3721 cattaccgga gcgacggcaa ctatcaggga gacgaaaatt atcgcgtggt ccataaattt
     3781 acaatcatat tagtatcccg caccgactat ttcaagaaga ccgcccgcgc cggcctttac
     3841 gcacgggaag agaaattcaa ggaaaccgga tacatgccgc ataccggctt tgacaatgcc
     3901 ggaaatgatg tcaaattaaa tcatcccgca taaaaatata gaaattcggt ttatcgacgt
     3961 ccgttcagtg attagacccc gaataactgg tatattaatg tttagagagt gattgagact
     4021 aaatgtccga cctgaaaacc gctcttgacc acgggctgac aaaagaagaa tacaggaaga
     4081 taacggatac gcttggcagg gagcccaaca taacggagct gggcatcctg gccgccatgt
     4141 ggtccgagca ttgctcctat aagagctcga gaatccacct tagaaagttc cccacgacgg
     4201 gcgaagcagt cattcagggt cccggagaga acgccggcat tatagatata ggcgacgggc
     4261 agtgcgccgt cttcaagatg gaaagccata accacccgtc cttcttagag ccataccagg
     4321 gcgctgcgac cggcgtaggc ggtatcctca gggacatatt cacgatgggc gctcggccga
     4381 tcgccgtact agactctctc aggttcgggg acccc
//
LOCUS       DASSJH010000131         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1000601, whole genome shotgun sequence.
ACCESSION   DASSJH010000131 DASSJH010000000
VERSION     DASSJH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000601"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFJ67_02845"
     CDS             complement(<1..280)
                     /locus_tag="VFJ67_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HET7289350.1"
                     /translation="MTVKVCRQQDGVAIIMALIMLLVMSVLATTVSFNSNNDFHSMSN
                     YKRGQEAFLAAENCIREGRNRFEIVGIEVLYFLLQNKGTVILGQGGAND"
     gene            complement(339..1583)
                     /locus_tag="VFJ67_02850"
     CDS             complement(339..1583)
                     /locus_tag="VFJ67_02850"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7289351.1"
                     /translation="MKNMLPLANSKGFTMIELMIAVSMLAIIIAGMTIALQQQQRQFK
                     FTKEAVDIDQTGRATLDYLATEIRNAGSRQGKTFAVQFINGGSAGDAPCTVDTDKAGS
                     ANSPPDCVTLYTWDISRGQEGTDLPSVPGIVEVAQNDPLQLRLPPQWFDEDTGMLIGE
                     AVQNPEILLGLRSRTALCNPDSTINCSLEPERCTECSVILRANVDDGTKTATIDSLED
                     IKVHNMPVSTFSGFSDFVNGTSVNGVNYGILPTFSSLPSEMTIVTSKTLSVDLTDNEL
                     LLQQNGSQPMPIAGGLNAAGIVDLQLVFNLQDANGRLTKVGVPLAANNGMYPDFTATA
                     LNGREQDIRSVEIYLVVKSKIKPQRMQGGQFTQTIPAIGDVLQRTVASPSNTINEPEE
                     GYTYRILSTTVYMRNHSREEFG"
     gene            complement(1595..2302)
                     /locus_tag="VFJ67_02855"
     CDS             complement(1595..2302)
                     /locus_tag="VFJ67_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7289352.1"
                     /translation="MTPLPDEKGFTFTEIMIAITIMALGFLAMAQMQFLSLRQKQLAE
                     EGTTASNIIQFIADRDMEEVKRAHLLNSIAFVEAQAGRLNPSSDSEPHLQYCKTSNSN
                     RVCDTCPCNPLAEVTPDPSQALQTTCAVIDVHDFDPKKVSFKSTVGQCSGSAGDSLIV
                     VKRVASNTDNTANPPETTLNVTYGIKTKNEFAETGLSSVSIKDTLATQNMVFSAHEED
                     WSQFIPAWTVVMVPHVP"
     gene            complement(2494..>3130)
                     /locus_tag="VFJ67_02860"
     CDS             complement(2494..>3130)
                     /locus_tag="VFJ67_02860"
                     /inference="COORDINATES: protein motif:HMM:NF039287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7289353.1"
                     /translation="GNVVDPYQMAEEFGADVFRYFLLREIPFGQDGDFSKNAIVSRVN
                     GDLANGLGNLVSRTLGMLERYRGGIIPAPAATGSSENNIAEASRSLIEEVDGAMSEIA
                     FHKALAAIWDFIALVNKYVDDEAPWTLAKEKKDERLDTVLWTISRSIGVVSVLISPFM
                     PSSAEEIWRRLGSTKPFSSLRLSDAGDPELIKPGQTVAKGSSLFPRYEEKS"
BASE COUNT          676 a          873 c          773 g          808 t
ORIGIN      
        1 gatcgttcgc ccctccctga cccagtatca ccgtaccctt gttctggagt agaaaataca
       61 gaacctcgat cccgacgatc tcgaacctgt tcctcccctc ccttatgcag ttctcggctg
      121 ccagaaacgc ttcctgacct cgcttgtagt tggacatcga gtggaaatcg ttattcgaat
      181 tgaaggacac cgtagtcgcg agcaccgaca tgactaggag cattatcagc gccattatta
      241 tcgcgacccc gtcctgttgt ctgcatactt tcacggtcat acaattcacg tgttggttct
      301 cattcttgtc attcatcatt atccgcacca tcacagtttt aaccgaactc ctccctcgaa
      361 tgatttctca tatagaccgt cgtcgaaagt atccggtacg tataaccttc ttcaggctcg
      421 tttatagtat tggacggcga agcgacagtc ctctgcagca catccccgat cgccggtatt
      481 gtctgcgtaa actggccgcc ctgcatcctc tgcggcttta ttttagattt gacaactaaa
      541 tatatttcga cggaccttat gtcctgttcc ctgccgttca gagccgtggc cgtaaagtcc
      601 ggatacatac cgttgttcgc cgccagcggg acgccaactt tcgtaagtct tccgttcgcg
      661 tcctggagat tgaaaacgag ctggagatcg actatgcccg cagcgttcag cccgccggcg
      721 ataggcatag gctgactgcc gttctgctgc aacagaagct cgttatcggt gaggtccaca
      781 ctgagcgtct tggaagtgac tatcgtcatt tcggacggca gcgaggagaa agtcggcaaa
      841 attccgtaat tcactccgtt gacggacgtg ccgtttacga agtctgagaa tccgctgaaa
      901 gtcgagacag gcatattatg gacttttatg tcttcgagcg aatctatcgt cgccgtcttc
      961 gtgccgtcgt cgacgttggc ccgcagaatt accgagcact ccgtgcatct ttcaggctcc
     1021 aggctacagt taatagtgct atccggattg caaagcgccg ttcgggacct caagccgaga
     1081 aggatttccg ggttttgcac ggcctcgcct atgagcatgc cggtatcctc atcgaaccac
     1141 tgcggcggga gtctgagctg gagcggatcg ttctgggcca cctcgactat acccggcacg
     1201 gacggcagat cggttccttc ctgaccccgt gagatatccc aggtgtatag agtgacgcag
     1261 tcgggaggcg agttggcgga gccggcttta tctgtatcca ccgtgcatgg ggcgtctccc
     1321 gcgcttccgc cgttgataaa ttgaaccgca aaggttttac cctgacgcga ccccgcgttt
     1381 cttatctccg tcgcgaggta atcgagtgtc gctctcccgg tttggtctat gtcgaccgct
     1441 tcctttgtaa acttgaactg cctctgctgc tgctggaggg cgatcgtcat gcccgcaatt
     1501 atgattgcca gcatcgatac ggcgatcatt agctctatca tcgtaaaacc tttactattc
     1561 gccaaaggaa gcatattttt catttatccc gtcctcacgg cacgtgcgga accatcacta
     1621 ctgtccacgc aggaatgaac tgactccagt cctcctcgtg cgcgctgaaa accatattct
     1681 gggtcgcgag cgtgtctttt atgctcacac tgcttaatcc tgtttctgca aactcgttct
     1741 tcgtcttgat cccatacgtc acgttcaaag tagtctcggg agggttggcg gtattatcgg
     1801 tattcgacgc gacccgtttc actacgatga gggaatctcc ggcgctcccc gagcactgtc
     1861 ccacagtgct cttgaacgac acctttttcg gatcgaaatc atggacgtct atgacagcgc
     1921 acgtcgtctg cagagcctgg ctcggatcgg gagtaacttc cgcaagaggg ttgcaggggc
     1981 atgtgtcgca aaccctgttc gagttgcttg tcttgcaata ctgcagatgg ggctcggaat
     2041 cgctgctcgg attgagcctg ccggcctgag cctcgacgaa agcaatggag ttgagcaaat
     2101 gagccctctt tacctcttcc atgtccctgt ctgctatgaa ctgtatgatg ttcgaggctg
     2161 ttgtcccctc ctcggcaagc tgcttctgcc ttagggatag gaactgcatc tgggccatcg
     2221 cgaggaaacc cagcgccata atggttatcg cgatcattat ctcggtaaaa gtgaaacctt
     2281 tctcatccgg aagcggagtc attttaacct cgtctctgcg agacctttga tggaattgca
     2341 agtaatatgc cagtttgaag ggatgcgcaa ctatccgaaa caattgcaga aatcgacgaa
     2401 gaaattaaga caaaagagag gaggaaatgc aaaaattgcc ctaattgtgc aaaaattgca
     2461 tataaattag ggtaaattac attatcggta taatcaggat ttttcctcgt atctgggaaa
     2521 caggctgctc cccttggcta cggtctgtcc gggtttaatc aattccggat cccccgcgtc
     2581 tgagagcctt aacgaagaaa acggcttagt ggagccgagt ctccgccata tctcttccgc
     2641 ggatgaaggc ataaacggtg aaatcagtac cgagaccact ccgatcgagc gcgatattgt
     2701 ccagagcacc gtgtcgagcc tttcatcctt tttctccttc gcgagcgtcc acggagcctc
     2761 gtcgtcgacg tatttattta cgagagcaat aaagtcccat atcgcagcca acgctttatg
     2821 aaacgctatt tcactcatcg cgccgtccac ctcctctata agcgagcggg atgcttccgc
     2881 gatgttgttc tcgctgctcc cggttgcggc gggcgcgggt atgataccgc ccctgtatct
     2941 ttcgagcatt ccgagcgtac ggcttacgag gttcccgagc ccgttcgcaa ggtcaccgtt
     3001 cacccttgag acgatcgcgt tcttcgagaa gtccccgtcc tgcccgaagg gtatctccct
     3061 aaggaggaag tatctgaata cgtccgctcc gaactcttcc gccatttgat atggatcgac
     3121 gacgttgccg
//
LOCUS       DASSJH010000132         6147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_678925, whole genome shotgun sequence.
ACCESSION   DASSJH010000132 DASSJH010000000
VERSION     DASSJH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678925"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..761)
                     /gene="pgl"
                     /locus_tag="VFJ67_02865"
     CDS             complement(27..761)
                     /gene="pgl"
                     /locus_tag="VFJ67_02865"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET7289354.1"
                     /translation="MKKEIYLFDDITGLAKRLALDFKKALEDKAVSGGSLTVALSGGH
                     TPKAFFEVLAAPPYGDSLPWDKVVFFWGDERCVPPDDDESNFKMTNLSLLSRIKIPEA
                     NIHRVLGENPPEKEAVRYAKEIKDNVPAGGNGFPAFDWIFLGMGEDGHTASLFPGAPT
                     LKEKEKICVVATHPQTGQKRVSVTFPVLDNGKRVSFLVAGEGKEAVLREILENPGREL
                     PYPASIVSPSDGVLEWYVDRLAAPWL"
     gene            complement(804..2342)
                     /gene="zwf"
                     /locus_tag="VFJ67_02870"
     CDS             complement(804..2342)
                     /gene="zwf"
                     /locus_tag="VFJ67_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7289355.1"
                     /translation="MAQDNSCIITIFGASGDLTKRKLMPALYALFRQGLLPKNFAILG
                     TSRSPFTDDAYGKKTIEDIVTYGGIKKSEQKQLEPFSKHVYYLAMDATDVNHYDDLKQ
                     KLAKIDEAQKTGGNYIFYLSTSPSLYTSIAKNLGKKGLQKEGKGDGWRRIIIEKPFGR
                     DLASAQALNHDLQRIFKEDQIYRIDHYLGKETVQDIFALRFSNGIFEPLWNRNYVSRV
                     EITAAETIGVEERGGYYDHYGALRDMIQNHLLQVLGTVTIEPPAFFDATDVRNETVKI
                     FQSLRPIKPEDVDKYAVRGQYTDSVINGKKIPGYRQERDVAHDSMTETFVALKLYIDN
                     WRWGGVPFYLRTGKCLPARVSEVVIDFKKTPHHLFTQEELCSARPNQLIIRIQPDEGI
                     LLRFGMKKPGAGFDIEEVGMDFHYSELADTYIPEAYERLILDCIMGDATHYARADAVE
                     ACWKFVDPILKEWEDDPEIKLYGYPVGTWGPPEAKHLFDCADDDWRYPTDHLTTDGDF
                     YVLK"
     gene            complement(2419..3075)
                     /gene="rpe"
                     /locus_tag="VFJ67_02875"
     CDS             complement(2419..3075)
                     /gene="rpe"
                     /locus_tag="VFJ67_02875"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7289356.1"
                     /translation="MVIIEPSILSADFTKLGEAVKEAGDAGVSWIQIDVMDGRFVPNI
                     NFGPGVVEALRPLTKLKLDVHLMIVEPEKYIKMFADAGADRIIVHQEVCYHLHRVLES
                     IRELGVQSGVTLNPGTPAAAIGEVLELADFVQVMGVNPGFGGQKFIWSQLDKIREIKN
                     MLGRKGLDVPIGLDGGIDRETAPEVVRAGATVLIAGSSVYNVRGSVRDNVKALLDSLR
                     "
     gene            complement(3103..3783)
                     /gene="rpiA"
                     /locus_tag="VFJ67_02880"
     CDS             complement(3103..3783)
                     /gene="rpiA"
                     /locus_tag="VFJ67_02880"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HET7289357.1"
                     /translation="MKKEAGISSVDLVRPGMVLGLGTGSTTRFALIEIAARLKDGRLK
                     DIVGIPSSRETEKTALELGIPVISFDEKQEIDLTIDGADEVDPQLNLIKGGGGALLRE
                     KVLAQSSRRNVMIVDESKLSPALGTHFPVPIEVIPFAWKPVANFLSFLGAEPVLRMKE
                     DGTPYTTDQNNYIIDSKFGPIRDLDDLALKLGQKAGIAEFGLFIGTASEVIVATSHGI
                     RYQKRNDS"
     gene            complement(3825..4580)
                     /locus_tag="VFJ67_02885"
     CDS             complement(3825..4580)
                     /locus_tag="VFJ67_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7289358.1"
                     /translation="MIKALIFDLDGTLVQTEVLKAESYARAAVELDKSLTEDEVIDAF
                     KDYVGLTRKEVAVGLIKRFNLESAAGARMKEYRVHTPWQAYVDMRLETYFKMISDPDI
                     LKDHLCPYNLGLLKWARGEGYPTGLGTMSHREEADRVLDVLGIRPEFDFIATIQDVEH
                     GKPNPEIYNLLADELKVPHADCLVVEDSASGVEAALAAHMNCIAVTTDFTREGVHKIP
                     PAGTLRVVDTPPVLLDTARSFISELDSQKEKTG"
     gene            complement(4590..>6147)
                     /locus_tag="VFJ67_02890"
     CDS             complement(4590..>6147)
                     /locus_tag="VFJ67_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289359.1"
                     /translation="HIPEVMADALPGIKKIVDEVRKDGYKNVLLLGMGGSSLAPLVLR
                     ETFGIKKGYLDVAVLDSTDPGAVLEQRNRLDMKKTLFIVSTKSGGTAETLSFMKYFYN
                     EVLKEAGKKETGRHFIAITDPGSGLQKIAAELKFRKTFLNDPNIGGRYSALSFVGIPP
                     AAFQGIDLDTLLARAITMLHNCESCNCAVDGDNSGAWLGAILGELAKAGHDKVTLVAS
                     PPIENFGSWVEQLIAESTGKDGKGILPVDREPLAQPEFYANDRLFVYLRLNDDATYDR
                     QVNALEKAGHPVVRINLKDIYDLGGEFFRWEMAIAVAGMIIGIHPFNQPNVEAAKVLA
                     RKMIAEYQKKGKLPVLKPTLRESGITVYSNFKAPNLEQALKKFLSFAKPGRDESKGRS
                     YVAFQAYVKPSDETYNALQKLRTKVERQYRMATTVGFGPRFLHSTGQLHKGDAGHGLF
                     IQFTSEKPEDAPIPDEPGKKASSISFGVFINAEALGDRQALLDRKRKVITFHLGEDVV
                     SGINRLAKIL"
BASE COUNT         1144 a         1796 c         1670 g         1537 t
ORIGIN      
        1 tcgccggagc gtgtcagcag tgctgtctaa agccagggag cggctaacct gtcgacgtac
       61 cactccaaaa caccgtctga aggactaact attgaggccg ggtaaggcag ctcccgtccg
      121 gggttttcca gtatctccct gagcaccgcc tccttccctt ctcccgccac gaggaacgaa
      181 accctctttc cgttgtcgag aacgggaaac gtaacgctca ccctcttttg tcccgtctgc
      241 ggatgagtcg cgacgacgca aatcttttct ttctccttca gggtgggcgc gccggggaaa
      301 agcgacgcag tatgcccgtc ctcgcccatg ccgaggaata tccagtcaaa tgcgggaaaa
      361 ccgttaccgc ccgcggggac gttgtccttt atttcctttg cgtaacggac agcctctttc
      421 tcgggagggt tctcgcccag cacgcggtgt atattcgcct cgggaatttt tatacgggag
      481 aggagggaga ggttcgtcat cttgaagttg ctctcgtcgt cgtcaggcgg aacgcagcgc
      541 tcgtcacccc agaaaaatac aactttatcc caggggaggc tgtctccgta tggaggcgct
      601 gcaagcactt cgaaaaaggc cttcggtgtg tgtcctcccg aaagcgccac tgtgagcgat
      661 ccgcccgaaa ccgctttatc ttcgagcgcc ttcttgaaat caagtgcaag gcgcttcgcc
      721 agccccgtta tatcgtcgaa cagataaatt tcctttttca tcacttctcc ttccgcccgc
      781 aaccctataa atccggtgac cggttatttg agcacgtaaa aatccccgtc cgtcgtgagg
      841 tggtcggtcg ggtatctcca gtcgtcatcc gcacaatcga agaggtgctt cgcttccgga
      901 ggaccccacg tgccgacggg atagccgtaa agcttgatct ccgggtcgtc ctcccattcc
      961 ttgagaatag gatcgacgaa cttccagcac gcttccacgg cgtcggcgcg ggcatagtga
     1021 gtggcatcgc ccatgatgca gtcgagtatg agccgctcgt aggcttcggg tatatatgta
     1081 tccgcaagct cggaataatg aaagtccatc ccgacctctt cgatatcgaa tccggcgcct
     1141 ggcttcttca tgccgaacct gaggagtatc ccctcgtcgg gctgtatgcg tattatgagc
     1201 tggttgggcc tggccgagca cagctcctcc tgtgtgaaga ggtggtgagg ggtcttcttg
     1261 aaatctatga ctacttcgct cacgcgggca gggagacatt tccccgtgcg gaggtaaaag
     1321 ggcacgccgc cccacctcca gttgtctatg tagagcttga gcgcgacgaa tgtctccgtc
     1381 atcgaatcgt gggctacgtc tctctcctgc ctgtaaccgg gaatcttctt accgttaatg
     1441 actgaatcgg tgtactgtcc ccttaccgca tacttgtcca catcctcggg ctttatcggt
     1501 ctcagcgact ggaatatctt gacggtttcg ttgcgcacgt cggtagcgtc gaagaaggcc
     1561 ggaggctcta tcgttaccgt gcccaacact tgaaggagat ggttctgtat catgtccctg
     1621 agcgcgccgt agtggtcgta gtacccgccc cgctcctcga ctcctatggt ttcggctgcc
     1681 gtgatctcga cgcggctcac gtagttcctg ttccagaggg gctcgaatat tccgttcgag
     1741 aacctgagag cgaagatgtc ctgtacggtc tctttgccga gataatggtc tatcctgtag
     1801 atctggtctt ccttgaatat cctctggagg tcgtggttga gggcctgcgc cgaggcgagg
     1861 tccctgccga acggtttttc tatgattata cgcctccagc cgtcaccttt cccttccttc
     1921 tggagaccct tcttgccgag gttcttcgct atagaggtgt agaggctcgg agacgtggac
     1981 agatagaata tgtaattgcc gcccgtcttc tgcgcctcgt ctatcttcgc cagtttttgc
     2041 tttaggtcgt cgtagtggtt cacgtcggtc gcgtccatgg cgaggtagta aacgtgcttc
     2101 gagaacggct cgagctgctt ttgctcggat ttcttgatgc ccccgtacgt aacgatgtcc
     2161 tcgattgtct ttttcccgta ggcgtcgtcc gtgaaagggg acctgctcgt gcccagtatg
     2221 gcgaaattct tgggcagaag cccctgcctg aataacgcgt acagcgctgg catgagcttg
     2281 cgtttcgtga ggtcgcccga agccccgaat atcgtgatta tacagctgtt gtcctgtgcc
     2341 atttcgctta cctctctctt acttgtattt ccgttcgctg aattaatatt tacccttaaa
     2401 acgcgtggcc gtcgcgcctc accgtaaact gtcgaggagc gctttaacgt tgtcccggac
     2461 actccccctg acgttataca cgctcgatcc tgcgatgagt acagttgccc cggcgcgcac
     2521 gacttcagga gccgtttccc tgtctatgcc gccgtcgagc cctatcggaa cgtccagccc
     2581 cttccttccg agcatatttt ttatctcgcg tatcttgtcg agctggctcc agatgaattt
     2641 ctgcccgccg aacccggggt tcacgcccat cacctgcacg aagtcagcta actcgagcac
     2701 ctcgcctata gcagccgcag gcgtgccggg attgagcgtt acgcccgact gtaccccgag
     2761 ctccctgatc gattcgagca cgcggtggag gtggtagcag acttcctggt ggacgatgat
     2821 gcggtcggct ccggcatcgg cgaacatctt tatatacttc tcgggctcga ctatcatgag
     2881 gtgcacgtcg agtttcagtt tcgtgagcgg cctgagcgcc tcgacgactc ccggcccgaa
     2941 gttgatgttc ggaacgaagc gtccgtccat cacgtctatc tgtatccagg agacgcccgc
     3001 gtccccggct tctttcaccg cttcgccgag tttcgtgaag tccgcagata atatggaagg
     3061 ctctattatg accatcgtgt cagtgtcctt ctaaaacgac aattacgaat cgttacgctt
     3121 ctgatagcgt atcccgtgcg acgtagcgac aatgacctcg ctcgcggtcc cgatgaataa
     3181 gccgaactcc gctatcccgg ccttctggcc gagcttgagg gcaaggtcgt cgagatcccg
     3241 tatcgggccg aacttgctat caattatata gttgttttgg tcagttgtgt atggagtccc
     3301 gtcttccttc atcctcaata cgggctccgc gccgagaaaa cttagaaaat ttgccacggg
     3361 cttccaggcg aacggaatca cctcgatggg gacgggaaag tgggtcccga gagcgggaga
     3421 gagcttgctc tcgtcaacta tcatcacgtt ccgtctgctc gattgcgcga gcaccttctc
     3481 cctcaggagc gcgccgccgc cgcccttgat gaggttgagc tggggatcga cctcgtcggc
     3541 tccgtctatc gtaaggtcaa tttcctgctt ttcgtcaaac gatatcaccg ggataccgag
     3601 ctcgagcgct gttttttccg tctcgcgcga gctggggatg cccactatgt ccttgagcct
     3661 tccgtccttg agcctggctg cgatctcgat gagcgcgaac ctcgtcgtgc ttcccgtgcc
     3721 cagtccgagg accatacccg ggcgcacgag atcgacggag cttataccgg cttctttttt
     3781 cagtctgtcc cggagcttct tctggtcggg ggaaagaggg tcgctcaacc ggtcttctcc
     3841 ttctgagagt ctagctcgct tatgaagctc ctggcggtat cgagaagcac aggcggcgta
     3901 tcgactaccc tcaatgtacc ggcgggaggt atcttatgaa ccccttccct cgtgaagtcg
     3961 gtcgtgaccg ctatacagtt catgtgcgcc gcaagcgcgg cctccactcc ggacgcggaa
     4021 tcttcgacga cgaggcagtc ggcatgcggc accttgagct catcggccag aaggttgtat
     4081 atctcggggt tgggcttccc gtgctccacg tcctggatag tagcgataaa atcgaattcg
     4141 ggccttatac cgagcacgtc gagcactcgg tcggcctctt ccctgtggga catggtcccg
     4201 aggccggtcg gatacccttc gcctcgcgcc catttgagga ggccgaggtt atagggacag
     4261 aggtggtctt ttaatatatc cggatcggat atcatcttaa aatatgtctc gagcctcata
     4321 tcgacgtagg cctgccaagg ggtgtgaacg cggtactcct tcattcttgc cccggctgcg
     4381 ctttcgagat tgaatctttt aatcagcccc acggcgacct ctttacgcgt gagtccgaca
     4441 tagtccttga aggcgtctat gacctcgtcc tctgtgagcg atttatcgag ctcgaccgca
     4501 gccctggcat aagactccgc cttgagtacc tccgtctgca cgagcgtgcc gtcgagatcg
     4561 aatatgagcg ccttgatcaa tttgccccct tatagtattt tcgcaagcct gtttattccg
     4621 cttacgacgt cttcgccgag atgaaacgtt attaccttgc gcttcctgtc gaggagcgcc
     4681 tggcggtcgc cgagggcctc agcgttaatg aacacaccga agcttatcga cgaggccttc
     4741 ttgcccggct cgtccggtat gggtgcgtcc tcgggcttct cggacgtgaa ctgtatgaat
     4801 agaccgtgcc ctgcgtcccc cttatggagc tgtcccgtag aatggaggaa cctcggcccg
     4861 aaccccactg tcgtggccat acggtattgc ctctccacct tcgtacggag cttctgaagg
     4921 gcgttatagg tctcgtcaga gggttttaca taagcctgga acgcgacgta gctcctcccc
     4981 ttcgactcgt cccttccggg ctttgcgaat gagagaaact tcttgagcgc ctgctcgagg
     5041 ttaggtgcct tgaaatttga atagaccgtt atgccggatt ccctgagggt cggcttgagt
     5101 acgggcagct tgcccttctt ctggtactcg gcgatcatct tccgggcgag gaccttggcg
     5161 gcctccacgt tcggctggtt gaacggatga atgcctatta tcatgcccgc gaccgcgatt
     5221 gccatctccc acctgaagaa ctctcccccg agatcgtata tatccttgag gttgatacgc
     5281 actacaggat ggcccgcctt ctccagagca ttgacctgcc tgtcgtaagt cgcatcgtcg
     5341 ttgagcctga gataaacgaa cagcctgtcg ttcgcataga actcgggctg agcgagaggc
     5401 tccctgtcga cagggagtat gcctttgccg tccttgccgg tgctctcggc tatgagctgc
     5461 tctacccagg agccgaagtt ctctatgggc ggggaggcga cgagggtaac tttatcgtgc
     5521 cctgcctttg cgagctcgcc gagtatcgct ccgagccagg cgcctgagtt atctccgtca
     5581 accgcgcagt tgcagctctc gcagttgtgg agcatcgtga tcgcgcgggc gagcagcgtg
     5641 tcgaggtcta ttccctggaa cgccgcgggc ggtatcccga cgaatgagag cgccgagtag
     5701 cgtcccccta tattggggtc gttgaggaaa gtctttctga acttgagctc ggcggctatt
     5761 ttctgaaggc cgcttcccgg atcagttatg gctatgaaat gccgtccagt ttccttcttg
     5821 cccgcctctt tcagcacctc gttgtaaaag tatttcataa aagaaagggt ctcggcggtg
     5881 cctcccgatt ttgtcgatac gatgaagagc gtcttcttca tatcgagacg gttacgctgc
     5941 tcgagaaccg ctccggggtc ggtgctgtcg agcacggcca cgtctaaata acccttcttt
     6001 atcccgaacg tctcgcgcag tacgagcgga gccaggctcg aaccgcccat tcccaaaaga
     6061 aggacgttct tatatccgtc cttcctgacc tcgtctacta tctttttaat gccgggaagg
     6121 gcgtcggcca tgacttcggg tatgtgg
//
LOCUS       DASSJH010000133         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_137474, whole genome shotgun sequence.
ACCESSION   DASSJH010000133 DASSJH010000000
VERSION     DASSJH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137474"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFJ67_02895"
     CDS             <1..1081
                     /locus_tag="VFJ67_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET7289360.1"
                     /translation="LAEVTDTKKPLPNIIVHHAIIKKGSLWVGDEVALRVNEKLRHGA
                     ETHHSTTHILHAVLREVLGKHVGQAGSLVAPGRLRFDFTHHSSVDDKELRKIEDIINE
                     RIRWDDRVVIDPDVPYDEAIKKGAMAFFQEKYGDKVRVVSIGEYSKELCGGTHLDSSG
                     EAGLFKIVSETASAAGVRRIEAVSGESAWNFMRGIEDRLHEASRILRVPESDVIPRIG
                     KLVEENESIRKELESVKSRMLSERTGDIMESVKKVNGLNVLSVEIQDADPDQLRKVWD
                     DIRPKLHSGIAVLGTKDDGKAYLLVGVTSDLHKKYHSGNIVKELAPVIGGKGGGRPDM
                     AQAGGNNPEKLQEALEKVFEILAAA"
     gene            complement(1095..1991)
                     /gene="rpoH"
                     /locus_tag="VFJ67_02900"
     CDS             complement(1095..1991)
                     /gene="rpoH"
                     /locus_tag="VFJ67_02900"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HET7289361.1"
                     /translation="MSQDTEKTKKKKQQGTLLPAHTTSLHRYLAEISNYPVLSREEEY
                     KLAKRYRDDGDLDAARKLVTSNLKFVVRVANEYKNYGFNTMDLIQEGNVGLMHAVKGF
                     DPTKGYRLISYAVWWIRAYIQNYIIRTWSLVKVGTTQAQRKLFYKLRSTKKELEMGGE
                     ALSTEDYREIAERLDVPDEAVVEMEQRMGSKDLSLDAELTDEKDASHLDFLTGGEASQ
                     EEILTKAQEEERVRSGMAVALKTLKDRERYIIEKRVLADEPLTLEELGSKFNISRERV
                     RQIESAALKKIKDVLEEKGISK"
BASE COUNT          440 a          551 c          592 g          476 t
ORIGIN      
        1 actcgccgaa gtcacggaca cgaagaagcc gctacccaat attatcgtgc atcacgcaat
       61 tataaagaag ggctcgctgt gggtggggga cgaagtcgct ctcagggtga acgaaaaact
      121 caggcacgga gcggagaccc accattcgac gacacatatc ctgcacgcgg ttctgaggga
      181 ggtgctcgga aaacacgtcg ggcaggcggg ctctctggtc gctcccggga ggctcaggtt
      241 cgatttcacg caccattcat ccgtcgacga caaggaatta aggaaaatag aagacatcat
      301 caacgagagg atcaggtggg acgacagggt cgtcatcgat cccgacgtcc catatgacga
      361 ggccattaaa aagggcgcca tggcgttttt ccaggagaag tacggcgaca aggtcagggt
      421 ggtcagcata ggggagtaca gcaaagagct atgcggggga acacacctcg actcctcggg
      481 cgaggccgga ctgttcaaga tagtatccga gactgcttct gccgcaggcg tgcgccgcat
      541 agaggccgta tcgggcgagt ccgcctggaa cttcatgagg ggcatagagg acaggctcca
      601 cgaggcgtcc cgcatcctga gggtccccga atcggacgtg ataccgagga tcggcaagct
      661 cgtcgaggag aacgagagca taaggaaaga gctcgaatcg gtcaaatcca ggatgctaag
      721 cgagaggacg ggagacatca tggagagcgt gaagaaggtg aacgggctta acgtcctctc
      781 ggtcgagata caggacgccg accccgacca gttaagaaaa gtgtgggacg acatcaggcc
      841 gaagctacat tccgggatag ccgtcctcgg cacgaaggac gacggcaagg cgtacctgct
      901 cgtaggggtt acgagcgacc tccataagaa ataccattca ggcaacatcg tgaaagagct
      961 tgcgccggtg ataggcggga agggcggggg gaggcccgac atggctcagg ccggcggcaa
     1021 caaccctgaa aaactgcaag aagctcttga gaaggttttc gagattctgg ctgcggcatg
     1081 agccgcttcc agcattactt acttattcct ttttcttcga gtacgtcttt tattttcttg
     1141 agagcggcgc tctctatctg tcttactctc tctctcgata tgttgaattt actgcccagc
     1201 tcttcgagag tgaggggttc gtcggcgagg acgcgcttct cgataatgta cctttccctg
     1261 tccttgagtg ttttgagcgc gacggccatt ccgcttctca ccctctcttc ctcctgggcc
     1321 ttcgtcagta tctcttcctg tgaggcctcg cctccggtga ggaagtcgag atgcgatgcg
     1381 tccttctcgt ccgtaagctc ggcgtcgagc gacaggtcct tgctgcccat tctctgctcc
     1441 atctcgacga ccgcctcgtc ggggacgtcc agcctctcgg ctatctccct gtagtcttcc
     1501 gtactgagtg cttcgccgcc catctctaac tcttttttcg tcgagcggag cttgtagaag
     1561 agttttcgct gcgcctgcgt cgtgcctacc ttaacgaggc tccatgtcct gattatatag
     1621 ttctgtatat acgcccttat ccaccagacg gcgtaggata tgaggcggta acccttggtc
     1681 gggtcgaacc ccttgaccgc gtgcataagc ccgacgttgc cctcctgaat gaggtccatg
     1741 gtgttgaatc cgtagttctt gtattcgttc gcgaccctta cgacgaactt cagattggac
     1801 gtgacgagct tgcgcgccgc gtcgaggtct ccatcgtccc tgtacctttt ggcgagtttg
     1861 tattcttcct cccggctcag aacgggataa ttgcttattt cggccaggta cctgtggagg
     1921 gacgtggtat gtgcgggaag caatgtgccc tgctgcttct tttttttcgt cttttcggta
     1981 tcctgagaca tgtgacctgt gtgatcgctt gattagtact aactagtata cgtttcgggg
     2041 gtgtcctata caacaaacg
//
LOCUS       DASSJH010000134         6447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_568028, whole genome shotgun sequence.
ACCESSION   DASSJH010000134 DASSJH010000000
VERSION     DASSJH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568028"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..839)
                     /locus_tag="VFJ67_02905"
     CDS             complement(6..839)
                     /locus_tag="VFJ67_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289362.1"
                     /translation="MNTFKYLFIALALSFIAGLPYKASSLEHIPFYATNNSGEDTSLC
                     CQGPWLRAACSLNSSESCLIKEGAVDYNFYSADVDIFSPYGSWKCCLSNDVSDCSGPV
                     QSPTPVECIGEIFDLEKGDTAVYITFTEDSLTVTPDNPSAASAAQASLAGESDTSTTQ
                     ASLGDDSSKPRRDRDTWSFQGTEGENVVITLEKIPESGQGGEQATLILQNGSAAIESK
                     TDDLPIEITTTLPSTGEYKLVVQQNDIPEEVRFRGKYYLSVKSSLGTVQDIKPSEDVE
                     Q"
     gene            complement(990..1763)
                     /locus_tag="VFJ67_02910"
     CDS             complement(990..1763)
                     /locus_tag="VFJ67_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HET7289363.1"
                     /translation="MTASQIEPLYSPSGEPMKAVVFLSGSGTNFIAIYEDYKRRHAEG
                     GKPYAEICAVFTNVPGCEGALKAAGFGIPVLSLSSKLFFSALGKDPDDDEARDYFDAA
                     SVSLIENVCKPDLIILAGYRRRLGGMILGRYRNRVVNLYPGDTTKSYLVRGVDASIQA
                     IRAGESSIKCTVYLEREHVRFGPALAQSEPISLAGYTEEDAEAVNGLILSKGEWKVFP
                     YAVHELIAGGRLGIDGDDNVCLDGRVLGKEGYQFGDFLA"
     gene            1825..2694
                     /locus_tag="VFJ67_02915"
     CDS             1825..2694
                     /locus_tag="VFJ67_02915"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET7289364.1"
                     /translation="MSNFWQRILTAAVAVPILYAIFRIGGILYLLFILALILVGQLEF
                     GKLLRSRNYPEQKISGMVFSLLLAVAAYTGYFYFMMAFTGAVVLVLVFELWKLREGGT
                     VLRAGVTLFGIVYIGWLLGHAVLLRNIGDIKPVAEFALNAQGLRDPGFFFIFFTVACT
                     FLNDTGAYFTGLRIGKRKLAPDISPGKTVEGTIGGIIACILTGIIVNYGFGSPLNSDW
                     TILFSLLIAVSAVFGDLVESAIKRGAGIKDTGDIVPGHGGVLDRFDSLIFVFPISYYF
                     VLVYYMSQGALLG"
     gene            complement(2691..3782)
                     /locus_tag="VFJ67_02920"
     CDS             complement(2691..3782)
                     /locus_tag="VFJ67_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289365.1"
                     /translation="MKGNAPFVRNLETLYYAEVFLVCAVSAVLGIRLFLHLTGYPQIG
                     NSTLHIAHLLWGGLLMLVSIVMLIAFIGRRSELWASVLGGAGFGMFIDEVGKFVTKDN
                     NYFFEPSVAIMYMVFVLIVISLQMIKSGWTFTKREYLINALKGLEEVALFDLDEDEKK
                     KVLGYLAKSDPDNPLTAPVRDLIVRTSPVPSHSPGIYTKAKKRFGKFYEGVAGSRYFS
                     AGITIFFLLQLIVTLGYVAIITLFIGFGKDRVMNVRALENITLRFDGLTFVDEARIFS
                     SFLSGVFVILGVYYIRKSRLLSLRMFEWSVLVSILLTYVFIFYKEQFAALAGLSFNLL
                     ILAGLRIMIRKEQADALVRGVSRDWGGFS"
     gene            3876..5024
                     /locus_tag="VFJ67_02925"
     CDS             3876..5024
                     /locus_tag="VFJ67_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7289366.1"
                     /translation="MKALESSSPMPSFEASKGPDGTLVMKIKGRLDAYTTGNIWKDSS
                     RELQASNPRKLIIDAGGIEYCDASGIALLIELKLLQEGKGGGFDLIGLSPDIEKLYRL
                     FKPDVLAQPEAAGIAPPGNIIERLGEKSWEVLEEIRVHVYFTGRVTVGLLTAALNPFK
                     VRWKEVFVTCEKFGANSLFIIGLVNFLVGLVIAFQSAIPMQQYGAQIFVADLIVIAYF
                     KELGPLMTAFVVNGRSGSAFAAELGTMKVNEELDALDTMGLDPVNFLVVPKVLAALFM
                     LPLLTVFGNLFGILGGLVVMLSMGFTVQTYINQITASGTYTMLLAGLFKTLFFAVLIA
                     GVGCLSGMRASRGPSAVGDAATNAVVTGIILMIVVDGIFGVIYYILGI"
     gene            5026..5799
                     /locus_tag="VFJ67_02930"
     CDS             5026..5799
                     /locus_tag="VFJ67_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7289367.1"
                     /translation="MNGGKDVIIEVKNFTAKYGDNVIIDDISFEVYKGEIFVILGGSG
                     CGKSTLLKHMIGLYKPAGGNIMIKGEDITEAEGRDRIDILKEIGVAYQLGALFGSMTV
                     LENVMLPLEEYTDLNVDARHLIGRMKLELVGLDGSEQKMPSELSGGMIKRAALARAMA
                     LDPEVLFLDEPSAGLDPITSAELDQLIIQLSRSLGVTFVVVTHELNSIYSIADRVIML
                     DKSTKNIIAEGKPEYLRDHSTIPVVQKFFRRQTDEQLGA"
     gene            5824..>6447
                     /locus_tag="VFJ67_02935"
     CDS             5824..>6447
                     /locus_tag="VFJ67_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7289368.1"
                     /translation="MSQKPDFFKIGLFVILALLLLAGAIILFGGGKFFERKVTVETYF
                     DQSVQGLSVGSALQFQGVQIGNVSYIGFVFTEYKTSRQYVLVRAEIYGNKVLGKGKRR
                     LYQTDKERVEGFKEMISQGLRLQLASQGITGVAFLNAVYLDPTRYHPLEHEWKPEYMY
                     IPSAPGTISQITETIEDLSDSIDNIDFKQISEEIEKLVVSLNKAVEDA"
BASE COUNT         1518 a         1737 c         1705 g         1487 t
ORIGIN      
        1 gacccttact gctcaacatc ttcagacggc tttatgtcct gtacggttcc cagagaggat
       61 tttacgctaa ggtaatattt tcccctgaac ctgacttctt cgggaatgtc gttttgctgc
      121 actaccagct tgtattctcc ggttgacggc aaagtcgtcg tgatttctat gggcaggtca
      181 tccgttttgg attctatcgc agcactcccg ttttgaagaa tcagagtagc ctgctctccg
      241 ccttgccccg attcggggat tttttcaaga gtaattacta cattttctcc ctcggtgccc
      301 tggaaactcc aggtatcacg gtcccgtctc ggcttactgc tgtcatcccc gagagatgcc
      361 tgtgtggttg atgtgtcgga ctctccggcg aggctggcct gtgcggccga tgcggcggag
      421 ggattatccg gagtcacggt aagtgaatcc tcggtgaatg taatatatac cgcggtatcc
      481 cctttttcga gatcaaatat ctccccaatg cattccactg gcgttggcga ttgaacggga
      541 ccggagcagt cggaaacatc gtttgacaga caacacttcc agctgccgta gggggagaat
      601 atgtcgacgt ctgccgaata aaagttatag tcaacggctc cctcctttat aagacatgat
      661 tcacttgagt tcaaggagca ggcggccctt aaccacggac cttggcagca taaactcgta
      721 tcttcgccgc tattatttgt ggcgtagaaa ggtatgtgtt caagagaaga cgctttgtat
      781 gggagacctg caataaaact caaagcaaga gctatgaaca gatatttgaa tgtattcatg
      841 aaaatcctct cctttaaata atactataac gtcgattttt taacgggtaa aataaagctc
      901 cttattcatt catcctgatc agctttcatt gtagttataa accccagacc ataatattat
      961 aaacagacac tacaattgaa agaggagatt taagcaagga aatctccaaa ttgataacct
     1021 tccttaccga gcacacggcc gtctagacag acgttgtcat ctccgtcgat cccgagcctg
     1081 ccgcccgcta taagctcgtg cacggcgtaa gggaagacct tccactcacc tttagagaga
     1141 atgagcccgt tcacggcctc agcatcctct tccgtatatc cggcgaggga tatgggttcc
     1201 gactgcgcga gcgccgggcc gaacctcacg tgctcccttt cgagataaac ggtacatttt
     1261 atggagctct ccccggcgcg tatagcctgt atggacgcgt cgacgcccct cacgagatag
     1321 ctcttcgtag tatcccccgg atagaggttc actaccctgt tcctgtaccg accgaggatc
     1381 atacctccga ggcggcgcct gtatcctgcg agaatgatga ggtcgggttt acatacgttc
     1441 tcgattagtg aaaccgaggc cgcatcgaaa tagtccctcg cctcatcgtc gtcggggtcc
     1501 ttaccgaggg cggagaagaa gagcttcgag ctcaggctca ggacaggtat gccgaacccg
     1561 gccgccttta acgcaccctc gcagccgggc acgttcgtga acacggcgca tatctccgca
     1621 taaggctttc cgccctccgc atgccgcctc ttatagtcct cgtatatggc gatgaagttc
     1681 gtccccgagc ccgaaaggaa gacgacggcc ttcatcggct caccggatgg gctgtaaagc
     1741 ggctctatct gagaggcagt catcggaggt aaaattcatt tgcaatatct tgcaagtcat
     1801 ggaaagtata tctattcgaa taacatgagc aacttctggc agaggatact tacggcggct
     1861 gtcgccgtgc ctattctcta tgcgatattc cgcatagggg gcatcttata ccttctcttc
     1921 atcttggccc ttatactcgt cggccagctg gagttcggaa agcttctgcg ctcgaggaat
     1981 tacccggagc agaaaatttc gggaatggtc ttttcccttc tcctcgcggt cgcggcatac
     2041 acgggatatt tctatttcat gatggccttc acgggcgcag tagtgcttgt cctcgttttc
     2101 gagctgtgga agcttaggga aggaggcacg gtgctgaggg cgggcgtcac gcttttcggt
     2161 atcgtatata tcggatggct cctcggccac gccgtgctcc ttagaaatat cggcgacata
     2221 aagcctgtcg cggagtttgc tctcaacgcc caggggctca gggacccggg ctttttcttc
     2281 attttcttca cggtcgcctg cacgttcctt aacgacaccg gggcgtattt cacggggctc
     2341 cgtatcggaa agagaaagct cgcgcccgat ataagccccg ggaagaccgt tgagggcacg
     2401 ataggcggga tcatcgcctg catactgaca ggcataatag tcaactacgg attcggctct
     2461 ccgcttaact ccgactggac catactcttc tcacttctga tcgcagtctc cgccgtgttc
     2521 ggagacctcg tcgaatcggc gatcaagaga ggggccggca tcaaggatac gggcgacatc
     2581 gtccccggcc acggcggcgt gctcgacagg ttcgacagcc ttatattcgt attccccata
     2641 tcctactact tcgtcctagt ctattacatg tcacaagggg cgctgctcgg ctagctgaaa
     2701 ccgccccagt cccgggacac ccctctcact aacgcatccg cctgctcctt ccttatcatt
     2761 atcctgagcc cggcgagtat gagcaggttg aaagaaagcc cggcgagggc cgcgaactgc
     2821 tccttgtaga atatgaatac gtatgtgagt agtatcgaga cgaggaccga ccactcgaac
     2881 atcctcagcg acaggagccg cgatttcctt atgtaataga ccccaaggat aacgaatacc
     2941 cccgagagaa acgacgaaaa tatccgggct tcgtcgacga acgtcaggcc gtcgaagcgt
     3001 aacgtaatat tctcgagcgc ccgcacgttc ataaccctgt ctttcccgaa ccctatgaaa
     3061 agagtgatga tggcgacata cccgagcgtt acaatgagct gcaaaaggaa gaagatagtt
     3121 atcccggcgc tgaaataacg gctgcccgca accccctcat aaaatttgcc gaaccgcttt
     3181 ttggccttcg tatatatgcc gggcgagtgg gatggaaccg gcgacgttct gacgatgagg
     3241 tccctcacgg gggcggtcag cgggttatcc gggtcgcttt tcgccagata tccgagcacc
     3301 ttttttttct cgtcctcgtc aaggtcgaag agcgccactt cctcgagccc cttgagggcg
     3361 tttatgagat attccctttt cgtgaacgtc cagccggact ttatcatctg cagcgaaatg
     3421 actatgagga cgaataccat gtacatgatc gcgaccgaag gctcgaagaa atagttgttg
     3481 tccttcgtga cgaacttccc gacctcgtct atgaacatcc cgaaaccggc tccacccagt
     3541 accgatgccc agagctcgct ccgtcttccg ataaacgcaa tcagcataac tatcgaaacg
     3601 agcataagga ggccgcccca gagcaggtgg gcgatgtgga gcgtgctgtt ccctatctgg
     3661 gggtatcccg tcagatggag gaagagtctt atacccaaaa cggccgaaac ggcgcatacc
     3721 aggaagactt cggcatagta taatgtctcc aggttcctga caaacggtgc atttcctttc
     3781 atatccttcg ccagtcttta ttctatgtta atcttacttg ttaatctacc gttttggtgt
     3841 catattatcg gtacgcaaaa cccgccaact ataatatgaa agctctcgaa tcctcttccc
     3901 ccatgccttc gttcgaggca tcgaaaggac cggatggaac cctcgtaatg aagatcaagg
     3961 gccgtctgga tgcgtacaca acaggtaaca tctggaaaga ctcttcccgg gaattgcaag
     4021 catcaaaccc acggaagctg attatcgacg ccggcggcat agagtattgc gacgcctcgg
     4081 gcatagccct tctcatcgag cttaagcttc tgcaggaggg gaaaggggga gggttcgacc
     4141 ttataggcct gagcccggac atcgaaaaac tctacaggct ttttaaaccc gacgtgcttg
     4201 cacaacccga agcggctgga atagcacccc cggggaacat tatcgagagg cttggcgaga
     4261 aatcgtggga agtgctcgaa gaaatcaggg tgcacgtata ctttaccggc cgcgtaacgg
     4321 tcgggctgct gacagctgcg ctcaacccgt ttaaggtcag atggaaggaa gtattcgtca
     4381 cgtgcgagaa attcggcgca aactcccttt tcatcatcgg cctcgtaaac tttctggtcg
     4441 gcctcgtaat cgcgttccag tcggctatac ccatgcagca gtacggcgcc cagatattcg
     4501 tagccgacct catagtcatc gcttatttta aagagctcgg cccgctgatg accgccttcg
     4561 tcgtgaacgg caggagcggc tccgccttcg ccgccgagct tggcacgatg aaggtgaacg
     4621 aggagctgga cgcactcgac acgatgggcc tcgacccggt caattttctg gtcgtgccca
     4681 aagtgctggc ggccctcttc atgctcccgc ttctcacggt cttcgggaac ctcttcggaa
     4741 tcctcggagg tctcgtagtc atgctctcga tgggtttcac cgtccagaca tacataaacc
     4801 agataacggc gtcggggacc tatacgatgc tgctcgcggg attattcaag accctctttt
     4861 tcgccgtgct gattgcgggc gtcggctgcc tgtccggtat gagggcttcg agggggcctt
     4921 ccgccgtcgg cgacgcggcg acgaacgctg tcgtaacggg gattatactt atgatcgttg
     4981 tagacggcat attcggagtc atatattaca tactcggtat ctgagatgaa cggcggaaaa
     5041 gacgtaataa tagaggtaaa gaattttacg gcgaaatacg gtgataacgt gatcatcgac
     5101 gatatttcgt tcgaagtcta caagggcgag atattcgtca tactcggagg gtcgggctgc
     5161 ggaaagagca cgctccttaa acacatgatc gggctctata aacccgccgg cgggaatata
     5221 atgataaagg gagaggacat cactgaagct gagggccggg acaggatcga tatattgaaa
     5281 gagatcgggg tcgcctacca gctgggcgca ttgttcggct ccatgacggt gctcgagaac
     5341 gtgatgctgc ctctcgagga gtatacagac cttaacgtgg acgcgagaca cctgatcggc
     5401 aggatgaagc tcgagctcgt ggggctagac ggctccgagc agaagatgcc ttccgagctg
     5461 agcggcggaa tgataaagag ggccgcgctc gctagggcga tggcgctcga cccggaggtg
     5521 ctgttcctcg acgagccctc ggcgggcctc gaccccatca catcggccga gctcgaccag
     5581 ctcatcatac agctatcgag gagtctcggc gtgacgttcg tcgtcgtcac tcacgagctt
     5641 aacagcatct actcgatcgc agacagggtc atcatgctcg acaaaagcac aaagaatata
     5701 atcgccgagg ggaaacccga atatctgagg gatcattcga ctatccctgt cgttcaaaaa
     5761 tttttcagga ggcagacgga tgaacagctc ggcgcgtaaa atcagaggcg gggaaaagaa
     5821 acgatgagtc agaaacccga tttcttcaag ataggattgt tcgtcatact tgcgctgctc
     5881 ctactcgcgg gggcgataat acttttcggc gggggaaagt tcttcgagcg gaaggtcacc
     5941 gtcgagacct acttcgacca gtcggtccag ggtctctccg ttggctcggc cctccagttc
     6001 cagggcgtac agatcgggaa cgtcagctac atcgggttcg tcttcaccga gtacaagaca
     6061 agcaggcaat atgtgctcgt aagggcggag atctacggca acaaggtact gggcaagggc
     6121 aagagacggc tctatcagac cgataaagag agggtggagg gttttaagga aatgataagc
     6181 caggggctca ggctccagct cgcgtcccag ggtattacgg gggtcgcgtt cctgaacgcc
     6241 gtatatctcg atccaacccg ttaccatccc ctcgagcacg agtggaagcc cgaatatatg
     6301 tatataccgt ccgcgccggg gaccatctcc cagatcacgg agacgatcga agacctctcg
     6361 gacagcatcg acaatatcga ctttaaacag atctccgagg aaatagaaaa gctcgtcgta
     6421 tccctgaaca aggcggttga ggatgcg
//
LOCUS       DASSJH010000135         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_787724, whole genome shotgun sequence.
ACCESSION   DASSJH010000135 DASSJH010000000
VERSION     DASSJH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787724"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2515)
                     /gene="ileS"
                     /locus_tag="VFJ67_02940"
     CDS             complement(<1..2515)
                     /gene="ileS"
                     /locus_tag="VFJ67_02940"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET7289369.1"
                     /translation="MSEAKKINYKDTLNLPRTDFPMKANLNQKEPEYQKRWKKIRLFE
                     RMREKSRGQEKFVFHDGPPYANGPIHLGHLLNKVLKDLVVRSKTMLGYDVDFVPGWDC
                     HGLPIEHKVLKELGDEAKELSKIKIRHKCQSYAEKYVKLQSGQMQSLGTVGDYEHPYL
                     TMTPDYEAGVLEVFSKLLERGLVYRDLKPVHWSIENRTALADAELEYYDRKDTSIYVL
                     FEIENPEGLPANLNLPADASPSLMIWTTTPWTLPANLAVAASPEGKYSLYRVTKDGKE
                     YYVVLVEDLAESVFKKTGTAEYEKLGTCTGRDMHDISVTYRHPFAGRMGKVVLAEYVT
                     FDEGTGLVHTAPGHGIEDYQTGLREGLDIYCPVLEDGTFDDTVPEWIRGQDVWEANGI
                     IIDRLRESGNLYYEEEYLHSYPHDWRSKSPTIFRATEQWFIGVDKRIEEFGGTLRELA
                     LEYSRSGIDFYPEWGRNRLRGMLEARPDWCISRQRAWGLPIPAFMNESGKTLLTPASV
                     NAVIEKIRQKGANYWFQASEEEILSGYDPAEDKDAPEWVKKKGALGRLKKGMDIFDVW
                     FESGSSWHSVLELRDIGYPADLYLEGSDQHRGWFQLSLLPALGVTGRPPFKALLTHGF
                     MVDAQGRKMSKSGGNALEVDELLKQHGADICRWWVSSLKYTNDIKVDWEYFKVAGEEY
                     RKVRNTIRFLLGNINDFDPEKDAVTFSEEDRYSIDAWAMGELGDFVRETREAYEGFQY
                     KRASELIYDFCYDTLSAVYLAATKDRLYCESAGSRKRRRSQTAMYHIADALIRLLAPI
                     LAHTADEAYLSLKGLKTDSPESVHLTDMPDVIEIDTDPNW"
BASE COUNT          433 a          788 c          671 g          644 t
ORIGIN      
        1 cccagttcgg gtccgtatct atctctataa cgtcgggcat gtcagtgaga tgcacacttt
       61 cgggcgaatc ggttttaagg cccttgaggc tcagatacgc ttcgtcggcc gtatgcgcca
      121 gtataggcgc gagcagcctg ataagcgcgt ccgctatgtg gtacatcgcg gtctggctcc
      181 tcctcctctt cctgctcccc gcgctctcac agtatagcct gtccttggtg gcggcgaggt
      241 ataccgcgct cagggtgtcg taacagaaat cgtatatgag ctcgcttgct ctcttgtatt
      301 gaaatccctc gtatgcctcc ctggtctcgc ggacgaaatc cccgagctcg cccatcgccc
      361 aggcgtcgat cgaataccgg tcctcttccg aaaaagtgac tgcgtccttc tccgggtcga
      421 agtcgttaat gttgccgaga aggaacctta tcgtgttcct gaccttcctg tactcctcac
      481 cagcgacctt gaaatactcc cagtccactt ttatatcgtt cgtatatttg agcgagctca
      541 cccaccacct gcagatgtcg gccccgtgct gcttcaggag ctcgtccacc tcgagcgcgt
      601 tcccgccgga cttgctcatc ttccttccct gcgcgtcaac catgaaaccg tgcgtgagga
      661 gcgccttgaa aggcggcctc ccagtcacgc cgagcgctgg caggagggag agctgaaacc
      721 accccctgtg ctggtcggag ccttcgaggt agaggtcggc cgggtatccg atgtccctca
      781 gctccaggac ggagtgccag gatgagcctg actcgaacca cacgtcgaat atgtccatac
      841 cctttttgag cctgccgagc gcgcctttct tctttaccca ctcgggcgcg tccttgtctt
      901 ctgcggggtc atagcccgag agtatctcct cttcgctcgc ctggaaccag taattcgcgc
      961 ccttctgtct tattttttct attacggcgt ttaccgaagc gggcgtgagt aatgtcttgc
     1021 cgctctcgtt catgaacgcg ggaatgggaa gcccccacgc cctctgcctg cttatgcacc
     1081 agtcgggtct cgcttcgagc attccccgga gcctgttccg tccccactcg ggatagaaat
     1141 cgatcccgct ccgcgagtat tcgagcgcga gctccctgag cgtgcctccg aactcctcta
     1201 tgcgcttgtc gacccctatg aaccactgct ccgtagccct gaatatcgtg ggcgatttgc
     1261 tcctccagtc gtgcgggtag ctgtggaggt actcctcctc gtaatagagg ttccccgatt
     1321 ccctgagccg gtcgattatt attccgttcg cctcccatac gtcctgcccg cgtatccact
     1381 cgggaacggt gtcgtcgaat gtgccgtcct cgaggacagg acagtatata tcgagccctt
     1441 ccctgagccc cgtctgatag tcctctatac cgtggccggg cgctgtgtgt accagacccg
     1501 tgccttcgtc gaacgtgacg tattccgcca gaaccacctt tcccatgcgc cccgcgaacg
     1561 ggtgcctgta tgtaaccgat atatcgtgca tgtcccggcc ggtgcacgtg ccgagttttt
     1621 catattccgc ggtcccggtc tttttgaaaa cgctctcggc aaggtcctcg acgaggacca
     1681 cataatattc ctttccgtct ttcgtgacgc ggtagaggct gtatttccct tcgggcgatg
     1741 cggcgaccgc caggttcgcc ggaagcgtcc agggcgtggt cgtccatatc ataagcgacg
     1801 gagacgcgtc agcggggaga ttaaggtttg cgggaaggcc ttccgggttt tctatttcaa
     1861 agaggacgta gatactcgtg tccttccggt cgtaatattc gagctccgcg tcggcaagcg
     1921 cggtgcggtt ctctatcgac cagtgtacgg gcttgaggtc cctgtaaacg agcccccgct
     1981 ccaggagctt cgagaacact tccagcaccc cggcttcgta gtcgggggtc atagtgagat
     2041 aagggtgctc gtaatccccg accgtcccca ggctctgcat ctggcccgac tgaagcttca
     2101 cgtacttttc cgcgtacgac tggcatttgt gcctgatctt tatcttggaa agctccttcg
     2161 cctcgtcgcc gagctccttc agcaccttgt gctctatggg aaggccgtgg cagtcccacc
     2221 cggggacgaa atccacgtcg tagccgagca tggtcttcga cctgacaacg aggtccttca
     2281 gcaccttgtt gagcaggtgt cccaggtgta tcgggccgtt cgcgtaaggg gggccgtcgt
     2341 ggaaaacgaa tttctcctgt cccctcgatt tctccctcat cctttcgaag agccttattt
     2401 tcttccacct cttctggtat tcgggctcct tctggttgag gttcgccttc atagggaagt
     2461 ctgtcctcgg aaggttaagc gtgtctttat agttgatttt cttcgcctcc gacatcggat
     2521 gctttctcct taagga
//
LOCUS       DASSJH010000136         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1543603, whole genome shotgun sequence.
ACCESSION   DASSJH010000136 DASSJH010000000
VERSION     DASSJH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543603"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VFJ67_02945"
     CDS             <1..1462
                     /locus_tag="VFJ67_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HET7289370.1"
                     /translation="RISILLTILFFCALPVSFTGEVHTARAGVLYAKNNAAPEPLRQE
                     SKAVDPAASGIVVIEINGMINPATLDYIRTGIEKAESGSADALVILLDTPGGLLNSTK
                     EIVKLMLNSPVPVVVYVSPKGASATSAGVFITLAANIAAMAPGTSIGAAHPVSLSPSN
                     EEPGKEEEPDTSKEKNKKGEKTRGTEESGDSDKDIMGEKLENYASSFIESIAEERGRN
                     VEWAEEAVRKSSSVTSTEALEKHVIDLIAPDLDSLIKDINGRKVKLPGGERVLKTDNP
                     EITRIAMNSKQKLIDILSTPDIAFLLLSLGSLGLLLEFYNPGLIFPGVAGAVCLLMGF
                     VSFQILPFNYAGIALLLLGIALFISEIYVTSYGFLTLGGIISFALGAILLFDTPESDL
                     RVGFDVVIASTLAFGLFFAYVIFYLIKAQRLSPQMGYEGLVGKEGSAVSDIHDSGKVY
                     IDGEYWDAVSDEPIRKGERVAVAEAPRGFRLKVKKI"
     gene            1477..2238
                     /locus_tag="VFJ67_02950"
     CDS             1477..2238
                     /locus_tag="VFJ67_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET7289371.1"
                     /translation="MFSPLVIFIGIIAILILSGVRVLNEYERGVVFRLGRIVEPKGPG
                     FKWIIPMVDKMTKISLRVITMDVPPQDIITKDNVSVKVNAVVYFRVLDPVKAVIQVEN
                     YLYATSQLAQTTLRSILGQVELDELLAERETLNIKLQEVLDKHTDPWGIKVTLVEVKY
                     VDLPQEMQRAMARQAEAERERRAKVISAEGEFQAATKLADASDILSRNPMSLQLRYLQ
                     TLLEMSSEKNTTIVFPLPIDLIRPILKSIDKAGGD"
     gene            2277..>3189
                     /locus_tag="VFJ67_02955"
     CDS             2277..>3189
                     /locus_tag="VFJ67_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7289372.1"
                     /translation="MKRILLSAFLGIAIISGLPACKSGEKKNETAPAESASDNTVTGP
                     VEGDWLIYHLSAEPATLNPITATDAYEGVVTGGNVYETLVKRDNKTLELIPLLAESWK
                     ISDDKLTYTFKIREGIKWQDGVPFTADDIVFSYKTIMDPKVDAPQLRSYYQEIKDVVA
                     LDDHTVRFTYARPYFLALEFCGGIPIVPRHIFEKGDFNTNPAGRAPVGTGPFKFVKWT
                     TGRDIVLERNKDYWGEKPHLNKIVFRIITDPSVALQVLKRQELDVASLTPIQWERQTG
                     SPDFEKNFDKFSYYTPNYSYIGWNSAKP"
BASE COUNT          793 a          893 c          912 g          591 t
ORIGIN      
        1 aagaatttcc atattactaa caatactctt tttttgcgcg ctcccggtat cgttcacggg
       61 ggaagttcac accgcccggg caggggtcct gtatgcgaaa aataacgccg ccccggagcc
      121 gctccgtcag gagagcaagg cggttgaccc ggccgcgtca gggatcgtcg ttatcgaaat
      181 aaacggtatg ataaaccccg cgacgctcga ttacatacgc acggggatag agaaagccga
      241 gagcggctcg gccgatgcgc tggtaatact gctcgacacg cccggagggc tcctcaactc
      301 gaccaaagag atagtgaagc taatgctcaa ctccccggtc ccggtcgtcg tttacgtttc
      361 ccccaagggg gcctcggcga cgtccgcggg cgtattcata accctcgccg ccaatatagc
      421 ggcgatggcc cccggtacga gcataggagc cgcccacccg gtctcgctct cgccttcgaa
      481 cgaggagccc ggcaaggagg aagaacccga cacaagtaag gagaagaaca aaaaagggga
      541 aaaaacccgg gggacggaag aaagcgggga ttcggacaag gacatcatgg gagagaagct
      601 cgagaactac gcctcgtcgt tcatagagag catcgctgag gagagaggga ggaacgtcga
      661 gtgggcggaa gaggcggtta ggaaaagctc ctccgtcact tccaccgaag cgctcgaaaa
      721 acacgtcata gaccttatcg cccctgacct cgacagcctc ataaaggata taaacggacg
      781 gaaggtaaaa cttcccggcg gtgagagggt gctgaagacg gataacccgg aaataacccg
      841 gatcgctatg aactcgaagc agaagctcat agacatactc agcacgcccg acattgcgtt
      901 ccttctcctg tcgctcggct cgctgggcct ccttctcgaa ttctacaacc ccggcctgat
      961 attccccggc gtcgcgggag cggtatgcct gctcatgggg ttcgtctcgt tccagatatt
     1021 gccgtttaat tacgcgggta tcgcgctcct tcttctcggc atcgccctct tcatttccga
     1081 gatatacgtc acgagttacg ggttcttaac gctcggaggt ataatcagct tcgcgctagg
     1141 ggcgatcctg cttttcgaca ctcccgagtc cgatctgagg gtcgggttcg acgtcgtaat
     1201 cgcctcgaca ctcgctttcg ggctcttttt cgcttacgtt atattctatc tgattaaggc
     1261 acagaggctc tcgccgcaaa tgggttacga gggactcgta gggaaagagg ggagcgcggt
     1321 ctccgatatc cacgactcgg gcaaagttta catcgacggc gaatactggg acgccgtgag
     1381 cgacgagccg atacgcaagg gggagagggt cgccgtggcc gaggcgccca ggggtttcag
     1441 gcttaaggta aaaaagatct agacaaggag gcgatcatgt tctcaccgct tgttatattc
     1501 atcggaatca tagcgatcct catactctcg ggcgtgaggg tcctgaacga atacgagagg
     1561 ggcgtggtat tcaggctggg caggatcgtc gagcccaagg ggccgggatt caagtggata
     1621 atcccgatgg tggacaagat gacaaagata agcctccggg tcatcacgat ggacgtcccg
     1681 ccccaggaca taatcacgaa ggacaacgta tcggtcaagg tcaacgccgt cgtgtatttc
     1741 agggtgctcg accccgtgaa ggcggtcata caggtcgaaa actacctcta cgccacctcg
     1801 cagctcgccc agaccacgct ccggagcatt ctcgggcagg tcgagctcga cgagctcctg
     1861 gccgagaggg agaccctcaa catcaagctc caggaggtgc tcgacaagca caccgacccc
     1921 tggggcataa aggtcacgct cgtggaggtg aagtacgtgg acctgcctca ggaaatgcag
     1981 cgcgctatgg cgcgtcaggc ggaggcggag cgggaaaggc gggccaaggt aatctcggcc
     2041 gagggtgaat tccaggcggc gaccaaactc gccgacgcgt ccgatattct gtcccgtaac
     2101 ccgatgtcct tacagttgag atatctccag acgctgctag agatgtcctc ggaaaagaac
     2161 acgacgatag tattcccact tcccatagac ctcataagac ccatactcaa gtccatagat
     2221 aaagcgggcg gcgattaatc ggacacatct aatcagacat cctcaaggag aataatttga
     2281 agagaatact gctatcagcg ttcctgggca ttgccataat ttcgggactc ccggcgtgta
     2341 agtccgggga aaagaaaaat gaaaccgccc cggcggaatc cgcatcggac aatactgtga
     2401 ccggtcccgt cgagggcgac tggctcatat accacctgag cgcagagccc gcgacgctca
     2461 atccgatcac tgcgactgac gcgtacgagg gggttgtcac gggcggaaac gtatacgaga
     2521 cgctcgtaaa gcgggacaac aagacgctcg agctcattcc gcttctcgcc gagtcgtgga
     2581 agatatccga cgacaagctc acttacacgt tcaagataag ggagggtatc aagtggcagg
     2641 acggcgtccc gttcacggcc gacgacatag tattctccta caagacgata atggacccca
     2701 aggtcgacgc cccccagctc aggagctact accaggagat aaaggacgtg gtcgcgctcg
     2761 acgatcatac ggtcaggttt acatacgcga gaccctactt cctcgctcta gaattctgcg
     2821 gcggaatacc catcgtcccg agacatatat tcgagaaggg cgacttcaac acgaatcccg
     2881 cgggaagggc ccccgtgggc acgggcccgt tcaagttcgt gaagtggacg accggaaggg
     2941 atatagtgct cgagaggaac aaggactact ggggtgaaaa gccacatctc aacaagatcg
     3001 tgttcaggat aatcacagac ccctcggtcg ccttgcaagt gctcaagaga caggagctcg
     3061 acgtcgcgag cctcaccccc atacagtggg agcggcagac gggctcgccc gatttcgaga
     3121 agaacttcga caagttcagc tactacaccc ctaactacag ctacatagga tggaactcgg
     3181 cgaagccct
//
LOCUS       DASSJH010000137         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_246913, whole genome shotgun sequence.
ACCESSION   DASSJH010000137 DASSJH010000000
VERSION     DASSJH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246913"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..512
                     /locus_tag="VFJ67_02960"
     CDS             288..512
                     /locus_tag="VFJ67_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289373.1"
                     /translation="MFEMVVTVLSRFIQSYSSMVLQLSKDWFILFKILFIPVSLYIYC
                     LTFLVIVTVLLIVVVIAFPLSFLSKLAFKQ"
     gene            602..1354
                     /locus_tag="VFJ67_02965"
     CDS             602..1354
                     /locus_tag="VFJ67_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET7289374.1"
                     /translation="MKGKLESLIDREKLPLHVAIIMDGNGRWAKNKNLDRISGHREGM
                     KSVRAVVTAAKDLGIRYITLYAFSAQNWQRPKNEVDALMELLKHYLLKERDKLIENGI
                     RLNAIGRLDELPQAVSDVLRETMEMTRDGREMTLTLALSYGGREEIMDAIKKIIAAGN
                     ISSEFIDHPDLSRFLYTEGMPEPDLLIRTSGEMRLSNFMLWQIAYAEIYVTRTLWPNF
                     RKRHLVKAILNYQNRERRFGLTGEQLKKREAI"
     gene            1351..>2244
                     /gene="dxr"
                     /locus_tag="VFJ67_02970"
     CDS             1351..>2244
                     /gene="dxr"
                     /locus_tag="VFJ67_02970"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET7289375.1"
                     /translation="MKNISILGSTGSIGRQTLEVVSRYPEKFRVVGLSAGENIGLLRE
                     QIREYRPEVVSVKDESLARTLRSEVGDDRVEILCGSEGASEVASLDINDLVVSAMVGA
                     SGLVPTLSAVKAGKDVALANKESLVVAGELLTTEAERSGVRIIPVDSEHSAIFQALET
                     CGAEYARRLIITASGGPFLNTPAEELGCVTVEVALNHPTWKMGDKITIDSATLMNKGF
                     EVIEARWLFGFEAERISVWIHPQSIVHSMVEYIDGSLISQMSLPDMRIPIAYALSYPE
                     RMYMNGHKVEPESFGELSFRGV"
BASE COUNT          577 a          567 c          625 g          475 t
ORIGIN      
        1 ctttatagag attaaccaaa catatattga tcggcaaccc ggatttgatc ctcggacagg
       61 aaacgtcctt atcgcgactc cgcctccgcg gaaatacgat ttattccatg atgccggtat
      121 taaacgatat ttctcgattg acccttccgg ggctgaaata agcatgatgc cgcaggtccc
      181 gtattcatac aggctgcata aaagctcccg cgcgaagtac cgactatcgg ttcccgattt
      241 gaagagtccc tgagattaat ataaatttat ccgctggagg cacatgcatg ttcgaaatgg
      301 tggttacagt tctaagcagg tttattcaga gctacagctc catggtgctc cagctatcga
      361 aggattggtt cattcttttc aagatacttt tcatacccgt atcgttatac atatactgcc
      421 tcaccttcct ggttatagtc acagtgctgc tcatagttgt cgtgatcgca ttccccctgt
      481 cgttcctgtc gaaactcgca ttcaaacaat agaccgtgag gagcggttaa tatacaaggc
      541 atatcagctt tgttaacaaa tatgaggtga tatacttaga ggtgatatac ttaaagggtt
      601 tatgaagggg aaactggaat cactcataga cagagaaaag ctgcccttgc acgtcgcgat
      661 aataatggac gggaacggga gatgggctaa gaataagaac ctcgacagga taagcgggca
      721 cagggaggga atgaaatccg tccgcgccgt ggttacggct gcgaaagacc tcggaattag
      781 gtacatcacg ctctatgctt tctcggctca gaactggcaa aggccgaaga acgaagttga
      841 cgcgctcatg gagctcctca agcattatct cctcaaggag cgcgacaagc ttatcgagaa
      901 cggcataaga cttaacgcga tcggcaggct ggacgagctc ccgcaggcgg tatccgatgt
      961 gctcagggaa accatggaaa tgaccaggga cggccgcgaa atgaccctta ccctggccct
     1021 cagctacggc ggcagggagg agatcatgga tgccatcaaa aaaatcatcg ccgccggaaa
     1081 tatatcctcg gaattcatag accatccgga tctctcgcgc ttcctgtaca cagaaggcat
     1141 gccggagccc gaccttttaa tccgcacgag cggcgagatg aggctctcga acttcatgct
     1201 atggcagatc gcctacgcgg agatttacgt aacgagaacg ctgtggccca atttcaggaa
     1261 gaggcacctg gtgaaagcca tcctcaatta ccagaaccgg gaacggagat tcggccttac
     1321 cggcgaacaa ctgaagaaaa gggaagcgat ttgaagaata tttcgatact cggctccacg
     1381 ggttctatag gaaggcagac cctcgaggtc gtatccaggt acccggaaaa gttcagggtg
     1441 gtaggacttt cggccgggga aaacatcggc ctcctccgag agcagatacg tgaatacaga
     1501 ccggaagtag tatcggtgaa ggacgagagt ctcgcgcgga cgctgcgctc ggaggtcggg
     1561 gacgaccggg tcgagatcct atgcggctcg gaaggggcga gcgaagtagc ctccctcgac
     1621 atcaacgacc ttgtcgtatc cgcaatggtg ggggcttcgg gtctcgtacc gactctgtcc
     1681 gcggtcaagg ccggaaaaga cgtcgcgctc gcgaacaagg aatcgcttgt cgtggcgggc
     1741 gagcttctga caaccgaagc tgaaaggagc ggcgtaagga taattcccgt cgacagcgag
     1801 cacagcgcga tattccaggc gctcgagacc tgcggcgcgg aatacgcccg cagactgata
     1861 atcaccgcct cgggcgggcc gttcctgaat acgcccgctg aggagctcgg ctgcgtcacc
     1921 gtggaggttg cgctcaacca tccgacctgg aaaatgggag acaagataac catcgattcg
     1981 gccacgctca tgaacaaggg gttcgaagtg atcgaggcga gatggctctt cggctttgag
     2041 gccgagcgca tctccgtgtg gatacatccg cagagcatcg tacactctat ggtagaatat
     2101 atcgacgggt cgttgatatc gcagatgagc ctccccgaca tgaggattcc gattgcttac
     2161 gctctttcct atccggaaag gatgtatatg aacgggcaca aggtcgagcc cgagagcttc
     2221 ggggagttaa gcttcagggg cgtc
//
LOCUS       DASSJH010000138         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_30428, whole genome shotgun sequence.
ACCESSION   DASSJH010000138 DASSJH010000000
VERSION     DASSJH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30428"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFJ67_02975"
     CDS             complement(<1..581)
                     /locus_tag="VFJ67_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HET7289376.1"
                     /translation="MGWIKSNILSSLPGVAHGFADRNADGSPAAVAESFGFSGIARLN
                     QVHGNDVFVLRDRSAVGGDRIADGDAIVTSLGGVGIGVSTADCVPILLTDALGGVAGA
                     VHAGWRGTVLRILDSVVEKINEEYGLGPSELNAVIGPSVGRCCYEVREDVASRIRDGF
                     EDWAEYLVPSGGSEYMLDLPGINRSVLERAGVR"
     gene            complement(585..1550)
                     /locus_tag="VFJ67_02980"
     CDS             complement(585..1550)
                     /locus_tag="VFJ67_02980"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7289377.1"
                     /translation="MNEERKKKTLIVPPELSGKRADSALPHLLEGLTRSQIKRLIEEK
                     CVLVGGEPVKPSRKLEAGDIVQAVLPEPEPLDVLPEDLPLDIIYEDEYIAVVDKPPGM
                     AVHPGAGVRSGTLVNALLYRCRDLSGIGGKIRPGIVHRLDKDTSGVMVVAKNDAAHNS
                     LVNQFKSRTVGKKYLAIVEGDLRKDSGEFSSKIGRHPVDRKRMSSKAKTGREAFTQWR
                     VIKRFKDAALVEAEPKTGRTHQIRVHFSENGFPILADAVYGHRKQKSPAIAAAAKKIG
                     RQALHASKIRFIHPQSGEIMEFTAPLPADLSEALNILSQSGENNL"
     gene            complement(1582..2673)
                     /locus_tag="VFJ67_02985"
     CDS             complement(1582..2673)
                     /locus_tag="VFJ67_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289378.1"
                     /translation="MPDSLRQFTDRDRPKELRFMAAQGLVPATPKDLTLILYYLTLDE
                     DTDVAGEARKTLKGMPSDVISTVLSDTGTQSGLLDYFARNLDDDGQLHRIALNNAASD
                     STVAHLAETTHSQSLIELIANNHERVARSDMIVEALSKNPAVTRSTLDAVIEFLKLYL
                     GRKFPFAGAPQAEEPLEPETDAAPAVDADTILESVTGSFLDDIELDDELVEDSGEEED
                     HHAPDVHNQSVMVKISMLNIGQKIKLSIMGNREARTILIKDPNRIVSKAVLKNPRLTD
                     SEIVLISQSKIVNEEILREIADSRKWARLYQVKLALVNNPKTPPHVSINLIRQLRDFD
                     LRNLRWNKNLPGVISTAVKQIIQDRKEKK"
     gene            2882..>4116
                     /locus_tag="VFJ67_02990"
     CDS             2882..>4116
                     /locus_tag="VFJ67_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7289379.1"
                     /translation="MAGDKNPVRQYEGRWATKPEQVKEGILFSYYSPGAGEVLLSGDF
                     NGWKRGTLRFTKGQDNVWRIILRLAPKRSYDYKYIVDGNWITDPNNPDMNPDIAGGAN
                     SIVYIGEKGNLLAGDDPERHKFTLEGRGIERTVFSSERYKRDFDLFYVLPPGYGGEPA
                     PAVICLNNYIKSQEIDVYCRKNGYIGIFPSPDVGGEYIRKGKLNIFSELLNDVKDRFK
                     IDDDRLYVTGMSNGGLEAYLVSLYFPDLIAASAVVFGPYRLRYYKDKIGHMNTDEVKK
                     FLGNLEFPQKMLPNLINTPIYISHGGGDEAVPADDAIVLHEILEKLGTPVKFNFYPNE
                     GHTWLMVDEDLPNVFDWFGKFRRNKYPERIYYNAPFGIFKNKIYWAEFMPYEVTQPVR
                     LRIGVTGRNGIRIESDNIK"
BASE COUNT          903 a         1187 c         1067 g          959 t
ORIGIN      
        1 ctccggaccc ccgccctctc gagcacgctc ctgtttatcc cgggaaggtc gagcatatat
       61 tcggacccgc ccgaagggac gagatactcg gcccagtcct cgaacccgtc cctgatacgc
      121 gaggcgacat cctcccggac ctcataacag cacctcccga cggaaggccc gataactgca
      181 ttcagctcgg aggggccaag cccgtattcc tcgttaattt tttcgacgac ggagtcgagt
      241 atccgcagta ccgttccgcg ccacccggcg tgcacagccc ccgcgactcc gcccagggcg
      301 tcggtcaaca gtatcggcac gcaatccgct gttgagacgc ctatgcctac acccccgagc
      361 gaggttacga tcgcgtcccc gtcggcgatc ctgtcccctc cgaccgcgga cctgtccctc
      421 aggacgaaga catcgttccc atgcacctga ttcagcctcg cgataccgga aaacccgaag
      481 gattccgcga cagcggcggg gctcccgtct gcattgcggt cggcgaaacc gtgcgcaact
      541 ccagggaggg acgaaagtat attcgacttg atccagccca tgacctagag attattttcc
      601 ccggattgag agaggatgtt aagtgcttcg ctcaaatccg ccgggagcgg agcagtaaat
      661 tccatgattt ctccgctttg gggatggata aagcggattt tagatgcatg gagagcctgc
      721 ctccctatct ttttagccgc tgccgcaatc gccggggatt tctgttttct atgaccgtaa
      781 acggcgtctg ccagtatagg gaagccgttc tcggaaaaat gcacccttat ttggtgggtc
      841 cttcctgttt ttggctcggc ctccaccagc gcagcgtcct taaacctctt tataaccctc
      901 cactgagtaa aggcctccct cccggtcttc gccttcgaag acatcctctt cctgtcgaca
      961 gggtgcctcc cgatcttcga ggagaattcc cccgagtcct tcctcaaatc gccctcgact
     1021 attgccaggt acttcttacc gaccgtcctc gacttgaact ggtttacgag cgagttgtga
     1081 gccgcatcgt tcttcgcaac caccatcacg cccgaggtat ccttatccag acggtgcacg
     1141 atgccgggcc ttatcttccc gccgatgccc gacaggtccc tgcatctata cagaagcgcg
     1201 ttaacgagcg tcccgctcct cactccggca cccggatgta ccgccatacc cggaggcttg
     1261 tccacaacgg cgatatattc gtcctcataa ataatatcga gcgggaggtc ctcgggcagc
     1321 acatcgagag gctcaggctc gggcaggacg gcctggacga tgtctcccgc ttcgagcttc
     1381 ctcgatggct ttaccggctc tcccccgacc agcacgcact tttcctctat cagtctttta
     1441 atctgcgacc tcgtaagtcc ctccagcaaa tgagggagcg ccgagtccgc cctctttccc
     1501 gaaagctcgg gaggaacgat caacgttttc ttttttctct cttcattcat aacggccggg
     1561 cacgaggggg cgggagataa tctatttctt ctcctttcgg tcctgtatta tctgctttac
     1621 cgctgttgaa atcacgcccg gaaggttctt gttccatctg aggtttctga gatcgaagtc
     1681 cctcagctgt cttattaaat ttatggaaac gtgcggaggg gtcttgggat tgttgacgag
     1741 cgcgagcttg acctgataga gtctcgccca cttcctgcta tcggctatct cccggagtat
     1801 ctcttcgttg actatcttgg attgagatat gagaacgatt tcactgtccg taagtctcgg
     1861 gtttttcagc accgctttgg acacgatcct gttcgggtcc tttatcaata tggtccgggc
     1921 ctccctgtta cccattatag agagcttgat cttctgacct atgttcagca tgctgatctt
     1981 gaccattacg ctctggttgt gtacgtcggg cgcatggtga tcttcctcct cgcccgagtc
     2041 ctctaccagc tcgtcgtcga gctcaatatc gtcgaggaag gacccggtca cgctttcaag
     2101 gatggtatcg gcgtccacgg ccggtgcggc gtcggtctcg ggttctaacg gttcctccgc
     2161 ttgcggagcc cccgcaaagg ggaacttcct accaaggtat agcttcagga attcgatcac
     2221 ggcgtcgagg gttgaccgcg tgacggcggg gttcttgctc aacgcttcca ctatcatgtc
     2281 agagcgcgcc acgcgctcat ggttattggc gataagctct ataaggctct gtgaatgggt
     2341 tgtttccgcc agatgagcga ctgtggaatc cgaagccgcg ttgttaaggg ctatccgatg
     2401 cagctggccg tcatcatcga ggttccgcgc gaaataatca aggagtcccg attgggtccc
     2461 cgtgtctgaa agcacggtcg aaataacgtc tgacggcata cccttaagag tcttccgggc
     2521 ctcccctgcc acgtccgtgt cctcatccag ggtcagataa tagaggataa gggtgaggtc
     2581 tttgggcgtc gccggcacta gaccctgagc cgccatgaat cgcagctcct tcggacggtc
     2641 cctgtcagtg aattgtctca gtgaatcagg catgttttca gtcattattg tcggtgtcga
     2701 atatcgtcat aaataccagg gttaaatata ccgtaaattt taaaaccggg cccattgcct
     2761 accatcctca aacccggtag tgtatcgaga ttcgacttgg gtcggagcat cttgacttta
     2821 tttcgcatta ggcacaattt cttattgata acgggtgttc gctgatagga ggaggaatca
     2881 catggcaggg gataaaaatc cggtcaggca gtacgagggc agatgggcga ccaaacccga
     2941 gcaggtgaag gagggtatcc ttttcagcta ttattccccc ggcgcgggcg aagtcctttt
     3001 atcgggggat tttaacggct ggaagcgcgg gaccctgagg ttcaccaaag ggcaggacaa
     3061 cgtctggcgc ataatactca ggcttgctcc gaagagatcc tacgattata agtacatcgt
     3121 cgacggcaac tggataaccg acccgaacaa ccccgacatg aaccccgata tagccggcgg
     3181 cgcaaactcg atcgtatata taggcgagaa aggcaacctg ctcgccgggg acgaccccga
     3241 gagacacaag ttcactctcg agggaagggg catcgagcgc accgtgtttt catcggaaag
     3301 atacaagagg gacttcgacc tcttttacgt tctgcctccc ggttacggtg gcgagcccgc
     3361 acccgctgtc atctgcctca acaattacat aaagtcccag gagatcgacg tttactgccg
     3421 gaagaacgga tacatcggga tattcccctc gcccgacgtc ggcggagagt acataaggaa
     3481 aggaaagctg aacatttttt cagagcttct gaatgatgtt aaagaccgct tcaaaattga
     3541 cgacgacagg ctctatgtaa cgggcatgtc gaatggtggt ctcgaggcat acctcgtttc
     3601 cctttacttc cccgacctca ttgccgcctc cgcggtcgta ttcggcccct acaggctcag
     3661 atattacaag gacaaaatcg gacatatgaa tacggacgag gtcaagaagt ttttagggaa
     3721 cctcgaattc ccgcagaaga tgctgcccaa cctcatcaac accccgatat acatatccca
     3781 cgggggcggg gacgaagcag ttcccgctga cgacgccata gtcctgcacg agatactcga
     3841 gaagctggga accccggtaa agttcaattt ttatccgaac gaaggccata cgtggctgat
     3901 ggtggacgaa gacctgccca acgtattcga ctggttcggc aagttcaggc gcaataaata
     3961 cccggaaaga atttactaca acgcgccttt cggaatattc aaaaataaga tttactgggc
     4021 ggaattcatg ccgtacgaag tgacccagcc ggtgaggctc cgcatcggcg tcacgggccg
     4081 gaacggcatc cgcatcgaat cggataatat caagaa
//
LOCUS       DASSJH010000139         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_788561, whole genome shotgun sequence.
ACCESSION   DASSJH010000139 DASSJH010000000
VERSION     DASSJH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788561"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /gene="sufB"
                     /locus_tag="VFJ67_02995"
     CDS             <1..836
                     /gene="sufB"
                     /locus_tag="VFJ67_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET7289380.1"
                     /translation="ELSTYFRINAESTGQFERTLIIAEEGSYVSYLEGCTAPKRDENQ
                     LHAAVVELIAHKDATIKYSTIQNWYPGNAEGEGGIYNFVTKRGRCVGRNSHISWTQVE
                     TGSAITWKYPSCILEGDGSVGEFYSVALTNRRQQADTGTKMIHIGKNTTSTIVSKGIS
                     AGHGQNTYRGLVQIGKDASGARNYTQCDSMLIGDKCGAHTFPYIEVKNSTARMEHEAS
                     TSKIGEDQIFYCQQRGLSAEDAVSLIVNGFCKEVFKELPFEFAVEAEKLLSISLEGSV
                     G"
     gene            890..1654
                     /gene="sufC"
                     /locus_tag="VFJ67_03000"
     CDS             890..1654
                     /gene="sufC"
                     /locus_tag="VFJ67_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET7289381.1"
                     /translation="MLEIRNLHVSVADQEIIKGLDLKVNAGEVHSIMGPNGSGKSTLA
                     QVLAGRESYVVTEGEITFEGKDLLELAPEERACEGIFMAFQYPVEIPGVANTYLLREA
                     LNSIRKYRGLEPLKHVEFAKLAKEKMGIMGMADNLLKRSVNEGFSGGEKKKNEIFQMQ
                     ILEPKLAILDETDSGLDIDALRVVADGVNALRKPERAFIVITHYQRLLEYIVPDFVHV
                     LVNGRIVKSGGKELALELEDRGYSWVEEEAKQAVNN"
     gene            1669..3012
                     /gene="sufD"
                     /locus_tag="VFJ67_03005"
     CDS             1669..3012
                     /gene="sufD"
                     /locus_tag="VFJ67_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET7289382.1"
                     /translation="MGINFSKEDDGYSRGFQEFRKEAPGKGITWMESLRDEGIKKFLE
                     LGFPTPENEDWRFTNVTPIARTVFSIGKNGHGHVSPAVLDKYTFPKLKTIRLVFVDGT
                     YTQELSNVSVLPEGVKVMSLASALSSEEELVKKYLSKYADFSKEAFTALNTAFMQDGG
                     FVYIPGGTRLKEPVHLLYVTVKSDNPTMTNHRNLIVVGDNCEANIIEHYVSLDESVYF
                     SNVVTELVIGENSSVGHYMIECESRKAFNVSTLRVEQGRSSNIRSHSVLLGGALVRNN
                     VHPVLAGEGCDSLINGLFMPTGRQHMDNYMVVEHVSPHCDSRQFYNGVLDGRSRGVFH
                     GRIIVHKDAQKTDAKQTNRNLLLSDHAQIDTKPQLEIYADDVKCTHGATIGQMDENAV
                     FYLRSRGINNQTARDILLGAFTSGTLESMQLEEIRNYCEGLVAEWFARRKQSEAD"
     gene            3049..>3251
                     /locus_tag="VFJ67_03010"
     CDS             3049..>3251
                     /locus_tag="VFJ67_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET7289383.1"
                     /translation="MAEYEKIAKTSDVPPGEIRSFVLGSEMVAVCNVDGRFYAFRDEC
                     THQAYPLSDGMLEGKKLTCIYHGA"
BASE COUNT          898 a          843 c          935 g          575 t
ORIGIN      
        1 tggagctttc gacctatttc aggataaacg ccgagagcac gggccagttc gaaagaaccc
       61 ttataatcgc ggaagaggga agctacgtga gctacctaga gggatgtaca gcccccaaga
      121 gggacgagaa ccagctccac gcggccgtcg tcgagctcat tgcccacaag gacgcgacga
      181 tcaaatactc gactattcag aactggtacc ccggcaacgc cgagggcgag ggcggaatct
      241 acaacttcgt caccaagagg ggaagatgtg tcgggcgcaa ctcgcacatt tcgtggaccc
      301 aggtggagac gggctcggca ataacatgga aatatccgag ctgtatcctg gaaggagacg
      361 gctccgtggg ggaattctat tcggttgcgc tgacgaacag gcgccagcag gcggacacgg
      421 gcacgaaaat gatacacatc gggaaaaaca cgacaagcac gatagtgtcg aaaggcattt
      481 cggcgggcca cgggcagaat acatacagag gccttgtgca gatcggaaag gacgcttcgg
      541 gcgcgcggaa ctacacgcag tgcgattcga tgctgatcgg ggacaaatgc ggagcgcata
      601 cattcccgta tatagaagtc aagaattcga cggcccggat ggagcacgag gcctcgacgt
      661 ccaagatagg cgaagaccag atcttttact gccagcagcg cggcctgtcg gccgaggatg
      721 ctgtatcgct tatcgtaaac ggcttttgca aagaggtctt taaggagctg ccgttcgagt
      781 tcgctgtaga ggcggagaag cttctgagca tcagccttga gggtagtgtc ggataataac
      841 tgccacccgg aattaatacc cgcagagaaa aaagctaagg agacaggaca tgctggaaat
      901 caggaatctg cacgtgagtg ttgcagatca ggaaataatc aaggggctcg acctcaaagt
      961 gaatgcggga gaagtacatt cgatcatggg cccgaacggg tcggggaaaa gtaccctggc
     1021 gcaggtgctc gccggccgcg aatcctacgt ggtgacggaa ggggaaatca cattcgaggg
     1081 taaagacctc ctcgagctcg cccctgaaga gagggcgtgc gagggcatat tcatggcgtt
     1141 ccaatacccg gttgagatac cgggcgtagc caatacttat ctactccggg aggcgctcaa
     1201 ctcgatccgc aaatacaggg gactcgagcc tctcaaacac gtggaattcg cgaaactcgc
     1261 caaggaaaag atggggataa tgggaatggc cgacaacctc ctcaaacgct cggtcaacga
     1321 ggggttctcc ggcggagaga aaaagaagaa cgaaatattc cagatgcaaa tactcgagcc
     1381 caagctcgct atactggacg agaccgactc ggggctcgat atcgacgcac tcagggtggt
     1441 ggccgacggc gtgaacgccc taaggaaacc cgagagagcg ttcatcgtga taactcatta
     1501 tcagaggctt ctcgaataca tagtgcccga cttcgttcac gtgctcgtca acggacgcat
     1561 agtgaaatca ggcggcaaag agctcgcgct cgagctcgag gacaggggtt acagctgggt
     1621 tgaagaggaa gcgaagcaag cggtcaacaa ttaaggagaa ttacccggat gggcattaac
     1681 ttcagtaaag aagacgacgg ttactccagg gggttccagg aattcagaaa ggaggcgcct
     1741 ggaaaaggaa tcacgtggat ggaatcgctt cgcgatgaag ggataaagaa attcctggag
     1801 ctcggattcc ccacgcccga gaatgaagac tggcgcttca cgaacgtaac cccgatcgcg
     1861 cggaccgtgt tcagcatagg gaagaacggg cacggacatg tttcccccgc agttctggat
     1921 aaatacacgt tcccgaaatt aaaaacaatc aggctcgttt ttgttgacgg cacttataca
     1981 caggagctct caaatgtatc ggtactgcct gagggcgtaa aggtcatgag cctcgcttcc
     2041 gctttgagca gcgaagaaga gctcgtaaaa aaatatcttt cgaaatacgc cgatttcagc
     2101 aaagaggcgt tcaccgcctt aaataccgcc ttcatgcagg acgggggctt cgtatatata
     2161 cccggcggca cacggcttaa agagcccgta cacctcctct acgtaacggt aaagagcgac
     2221 aaccccacaa tgacgaacca caggaacctc atcgtcgtcg gagacaactg cgaggcaaac
     2281 ataatcgagc actacgtgtc cctcgacgag agcgtctact tctccaacgt ggtgacggag
     2341 ctcgtcatcg gagaaaacag ctctgtcggc cactacatga tagaatgtga gagcaggaag
     2401 gcttttaacg tctcgactct ccgcgtagag cagggcagaa gctccaacat acgctcccac
     2461 tcggtactcc tgggaggggc gctcgtaagg aacaacgtcc atccggtgct cgccggagag
     2521 ggctgcgact ctctcatcaa cgggctcttc atgccaaccg gaaggcagca catggataat
     2581 tacatggttg tcgagcacgt gagccctcac tgcgacagca ggcagttcta caacggcgta
     2641 ctcgacggca ggtcgagagg cgtgttccac ggccgcatca tagtacataa agacgcgcag
     2701 aaaacggacg cgaagcagac gaaccgcaac ctcctgcttt cggaccacgc gcagatagac
     2761 acgaagcccc agctcgagat atacgccgac gacgtaaaat gcactcacgg cgcaacgata
     2821 ggacagatgg acgagaacgc cgtattttac ctccgctccc gcggtatcaa taaccaaacc
     2881 gcacgggaca tcctcctggg cgcgttcacg agcggcactc tcgaaagcat gcagctcgaa
     2941 gagataagga attactgcga agggctcgtc gccgagtggt ttgcccgcag aaaacagagt
     3001 gaagccgatt agtccgaatt cccgaaagaa aaaccgggga tcaataccat ggccgaatat
     3061 gagaagatag cgaaaacatc cgacgtgccg ccgggtgaaa taaggtcttt cgtgctcgga
     3121 agcgaaatgg tcgcggtctg taacgtggac ggccggttct atgccttcag ggacgaatgc
     3181 acgcaccagg cgtaccccct ctcggacggc atgctcgaag gaaaaaaatt aacctgcata
     3241 tatcacgggg c
//
LOCUS       DASSJH010000140         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_31268, whole genome shotgun sequence.
ACCESSION   DASSJH010000140 DASSJH010000000
VERSION     DASSJH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31268"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFJ67_03015"
     CDS             complement(<1..333)
                     /locus_tag="VFJ67_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7289384.1"
                     /translation="MEFVHKFFSAIPRDDLLIYKDDVTKLESLENWFLNPNYEKVVEL
                     VTVCGKEVVAKGTLHTQGLYWPHAAEVKLVVHPDYRGKGLGSQMFNLLLYEGLKHQFQ
                     KIIVRYIPD"
     gene            complement(685..2127)
                     /locus_tag="VFJ67_03020"
     CDS             complement(685..2127)
                     /locus_tag="VFJ67_03020"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HET7289385.1"
                     /translation="MKSLPKKLKPGSLILLFLSMCVMSGLSGCASYCANQDTGDESMF
                     STPPAKGCKVYSPSTEPTRDVPFKLRAEKQTEDGISVSAVVLSDEESERVFGVNLAGN
                     GIQPVWLEIENTTEKPVTLIYVATDPGYYSPNEAAYTNYSYASPVNEEMNEYFNKQSI
                     KREIPAGGVLSGFFYTNWDPGVKYLNVSIYGEDQEKSFVFYFVIPGIKLDYQRVDWDS
                     LYKKDEYVNYENEDELRKALEAVPCCATRKDGTGENDALNFFVIGDTDNIVSAFIRRG
                     WDVTEPISASSGWRAFKAYFSGTRYRTSPMSTVFVFRRAQDIGLQKARSTIHERNHLR
                     LWLMPVRYQGMDVWIGSVSRDVGSYFTFRTPWLSAHAIDPDLDEARTYLVQDLLFSGG
                     VEKFGYIKGIEPATPDNPHRNFMKQPWWTDGLRAVFLFGDEPVTLNELSFFDWEWAGE
                     DGPQVIEFIKAMSAKKNTENQAPPADTKTD"
     gene            complement(2133..>2776)
                     /locus_tag="VFJ67_03025"
     CDS             complement(2133..>2776)
                     /locus_tag="VFJ67_03025"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HET7289386.1"
                     /translation="DPDDISSAIVRRKWDVSEAETNKFKFDFARAFSPASYRTFPMAS
                     LYLYGRRQDVSFQKSRRESDATYRQRNTFRLWLTPIRYRGKTVWAGSVSRDIGSDLRI
                     RKYWFAAQEIDPDIDETRDYMVEDLVLTGSVQKLGFANGVGESTPEDPSYSLDKLPWW
                     SDGYRAVLLFGKDQVTLSDLDYFGWEDVNIVYDEETGVDGTGGASPDEGEKGK"
BASE COUNT          599 a          781 c          689 g          707 t
ORIGIN      
        1 gtccggaatg tacctcacga ttattttctg gaattggtgt ttgagccctt catagaggag
       61 caggttgaac atctgggaac cgagaccctt gcccctgtaa tccggatgga ccacgagctt
      121 cacttcggcg gcgtggggcc agtatagccc ctgagtgtgg agcgtgcctt tcgcgacgac
      181 ctctttcccg caaacagtca cgagctcgac caccttctcg taattcgggt tcaggaacca
      241 gttttcaagc gattccaact tagtaacgtc gtctttataa ataaggaggt catccctggg
      301 tatcgccgaa aagaacttat gtacgaattc caagtcctcc tcaagaatag gcctgataat
      361 aacactgcta ttatctttga gcaaaaactc ttttggatac actttcatcg ttgatagtta
      421 tcttgcaagg agcataccaa gtgaaaaatt ggccgcttca tgtgcgcaga tgcggtctca
      481 gactgactct tgccggttgg aatatatgtt gatattatag aataattcaa tcgtagacgt
      541 atattagaaa cgcattagcg tagaaagaaa actcaattgc tttttggcgt cctctaacag
      601 tattgtcgta atataggaca catgacatcg tgtaactgag cggcgtccga gaggtagtta
      661 taatcaggct catgcgtcag taaatcagtc ggttttcgta tcagccggag gagcctgatt
      721 ctctgtattt ttctttgcgc tcatggcttt aatgaattca atgacctgag ggccgtcctc
      781 cccggcccac tcccaatcga agaacgaaag ctcgttgagc gtaaccggct cgtcgccgaa
      841 caggaaaaca gccctcaatc cgtcggtcca ccacggctgc ttcatgaaat ttctgtgggg
      901 gttatcgggt gtcgccggct ctatgccctt gatataaccg aatttctcga ctcccccgga
      961 gaatagcagg tcctgcacca gatatgtcct cgcttcgtcc aggtcagggt ctatcgcatg
     1021 agccgaaagc cagggcgtcc tgaaagtgaa gtaagagccc acgtccctgc tcacggaacc
     1081 tatccacacg tccatgccct gatacctgac gggcatgagc cagaggcgga ggtggttcct
     1141 ctcgtgtata gtggacctcg ccttctggag acctatgtcc tgggccctcc tgaatacgaa
     1201 gacagtgctc atgggggatg tcctgtatct cgttcccgag aaataagcct tgaacgctct
     1261 ccagcccgag ctcgccgata tcggctcggt tacgtcccaa cccctcctta tgaaggcgga
     1321 gacaatgttg tccgtatccc ctatgacgaa gaaattaagg gcgtcgttct cgcccgtgcc
     1381 gtcctttctc gtggcgcagc acggcacggc ctcgagcgcc ttgcggagct cgtcctcgtt
     1441 ctcatagttc acatattcgt ctttcttata taaagagtcc cagtccaccc tctgataatc
     1501 gagcttgatg ccgggtatga caaagtagaa aacgaagctc ttctcctgat cctcgccgta
     1561 tatgctgacg ttaaggtact tgacacccgg gtcccagttc gtatagaaaa agcccgataa
     1621 gaccccgccg gccggaatct ccctcttgat gctctgcttg ttaaagtatt cgttcatctc
     1681 ttcattcacg ggactggcat agctgtagtt cgtgtacgcc gcctcgttcg gggagtagta
     1741 tcccgggtct gtcgcgacat aaatcagggt aaccggcttt tcggtcgtgt tctcgatctc
     1801 gagccagacg ggctgaatgc cgttcccggc caggttgacg ccgaataccc tctcgctctc
     1861 ctcatcgctc agaacgacgg cggaaacaga aataccgtct tcggtctgtt tctccgccct
     1921 gagcttgaac ggcacgtccc tcgtaggctc cgtggagggt gaatagactt tgcatccctt
     1981 ggccggaggc gtgctgaaca tgctttcgtc cccggtgtcc tggttcgcgc agtaggacgc
     2041 gcatcccgat agcccggaca tcacgcacat cgaaagaaaa agtaaaatca gactccccgg
     2101 cttgagcttt ttcggtaagg atttcatgcg gattattttc ccttttctcc ttcgtcgggc
     2161 gaagccccgc ccgtaccgtc cacgcccgtt tcctcgtcgt aaactatgtt cacgtcttcc
     2221 catccgaagt aatcgaggtc gctgagagtc acctggtctt tcccgaagag cagaacggcc
     2281 ctgtaaccgt ccgaccacca tgggagcttg tccaggctgt agctcgggtc ttcgggcgtt
     2341 gactcaccga cgccgtttgc gaaacctagc ttttgcacgc tccccgtcaa cacgaggtct
     2401 tccaccatat agtctctggt ttcgtctata tcggggtcta tctcctgggc ggcgaaccag
     2461 tatttcctga tcctgagatc ggagccgatg tccctgctca cggaccccgc ccagactgtc
     2521 ttgccccggt atcttatcgg cgtgagccag agcctgaacg tattacgctg acgataagtt
     2581 gcgtctgact cgcgcctaga tttctggaag ctcacgtcct gcctcctgcc gtagagatat
     2641 agactggcca tcggaaaagt cctgtagctc gcgggggaaa aggcgcgtgc aaaatcgaat
     2701 ttgaatttat ttgtctccgc ctcggatacg tcccatttcc tccgcactat ggccgaagaa
     2761 atgtcgtccg ggtctc
//
LOCUS       DASSJH010000141         4940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_789098, whole genome shotgun sequence.
ACCESSION   DASSJH010000141 DASSJH010000000
VERSION     DASSJH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789098"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFJ67_03030"
     CDS             <1..201
                     /locus_tag="VFJ67_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7289387.1"
                     /translation="VGEQTGALEDMLNKIADFYEEEVDIAVASLLSALEPIMITVLGA
                     TVGVIVVSMYLPMFKLVATMAN"
     gene            314..1954
                     /locus_tag="VFJ67_03035"
     CDS             314..1954
                     /locus_tag="VFJ67_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7289388.1"
                     /translation="MKVLQNPYGGLKSSVILRVVFTTVLLGSGAVIYYGRGARKEAFY
                     LAVIVAVIYFLSLIALLLQTLYQRYPRIFKITQLVFDLALASIVIFVTGGKSSPFIFL
                     YVLIIIYSSIVLNKAASYVTALISGLVYVLIVFYQIRIETPLPEGQSILSSVSKWGEI
                     GLVSTYFHLTGFLLVAILSGYLSDRFRAAGRELGESERSLRILQNLHQNIIESLTSGM
                     ITLDLEGRIISINRTGLEILGINGEDRILGKDLGQFMSGLHLEALVSKKREQMLYTAP
                     DGRMVTLGFSTSELKDTEDKTQGYIIIYQDLTEVKELEDRLRTSEKMALLGQLAAGLA
                     HELRNPLSAISGAVEILGSDVKPTEENLRLVRMATQEVERLNLLVEDFLILTMPIQKL
                     TTQVDLGLIISDTVESFTKTIRRGNIEIINQVEKGIYVQADSYRLKQAVWNLLLNSVD
                     AMPIGGLIMIKSRTEDNSAIIEISDEGKGVDENFISRIFEPFFTTKEVGTGLGLAIVQ
                     KVIEGYNGNINVISSRGNGATFVITLPRIKGVPADVIH"
     gene            1974..3392
                     /locus_tag="VFJ67_03040"
     CDS             1974..3392
                     /locus_tag="VFJ67_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7289389.1"
                     /translation="MGHNDSNILIVDDEPGMREFLEIMLQKDGYSVDTAADGSEAIDK
                     IEGSLFDLAIVDIQMPVLNGIEVLKKFNEKSPDTTVIMITAYASHETAIEAMKLGAYD
                     YITKPFKIDEIKLVIKKALEKKKLERENTRLRKELETQYGFGNIIGRSPSIVKVFELI
                     KRVSELNVSVLITGESGTGKELVAKAIHYSGSRHEKPFVPVNCGAIPESLMESELFGY
                     KKGAFTGAVRDKRGLFEEAHGGTIFLDEIGDLPLHLQVKLLRVLEEKKIRPLGGTEAV
                     NIDVRVIAATNKKLEDEVTSGRFREDLFYRLNVIKIVLPPLRERKIDVSPLAIHFINK
                     YSVDMEKDIRGISPKALEILENYHYPGNVRELENIIARCVALESSNVIRQETLPQLVT
                     GRDYLDLDTSFASNSSLDTLLGDVEKKMIEKALRTTNGNKTEAARLLGITLRSLRYRL
                     AKHELDDGNGEDLDDMDEAGEL"
     gene            complement(3427..3903)
                     /locus_tag="VFJ67_03045"
     CDS             complement(3427..3903)
                     /locus_tag="VFJ67_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET7289390.1"
                     /translation="MFLLMKVSGIALDPFTNTPIVILKDAANEKTLPIWIGFMEASSI
                     AMELEKTPRIRPITHDLVKNLLEKLKFIVTKIEVTDLRDDTFYARIYLKRENEEYSLD
                     SRPSDAIAIALRTDSPIYVNDEVIEKSKKIEIDEDKEKLAEILDKIPEGDFGKYKM"
     gene            complement(3923..>4940)
                     /gene="miaB"
                     /locus_tag="VFJ67_03050"
     CDS             complement(3923..>4940)
                     /gene="miaB"
                     /locus_tag="VFJ67_03050"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET7289391.1"
                     /translation="FVLGPRAIPRLPELIKKIEREKHRAVETSYDIQELFDIEPYHSE
                     GKVTAFVSVQQGCNKRCSYCIVPYVRGNEVNRPLEDILRETRSLVDKGVCEVTFIGQT
                     VNSWKENGYKFGDLLRAAADVEGLDRIRFTTSYPRDITARMIEAVRDVPKICRHIHLP
                     VQSGSNAVLGRMKRTYTREWYSDTVKRLKDAVPDMAVSTDIIVGYPGETERDFSDTMS
                     LLEEIEFESAFSFKFSPRPGTPAAACPGDEVVDEDTAGRRLSMLQSFQRDVTLRKNRA
                     RVGQIEEVLAEGASKNDPGVLTGRTSHNRITHFKGDSGLSGKIIKVRITEGLFNSLRG
                     ELSV"
BASE COUNT         1172 a         1290 c         1332 g         1146 t
ORIGIN      
        1 gtaggggagc agacgggcgc gctggaggac atgcttaata agatcgccga cttttacgaa
       61 gaagaggtgg atatcgccgt tgcctcattg ctatcggcgc tcgagccgat aatgataacc
      121 gttttagggg ccacggtcgg agtcatcgtc gtttcgatgt accttccgat gttcaagctg
      181 gtagcgacca tggcgaatta aacccctcac cggctgccgg cgcttaacat gcccgtatcc
      241 ttaattaatt gatagatagg ggtgctaatc gctcttgtca caattcagcc gaaggtatag
      301 tcttaaaacg gatatgaaag tcctacagaa cccctacgga ggattaaaga gctcggtaat
      361 actgagggtg gtttttacga ccgtgctgct gggttcgggg gccgtaatct attacggcag
      421 aggcgcgaga aaggaggcct tttacctggc cgtaatcgtg gccgtaattt atttcctgag
      481 cctcattgct cttctgcttc aaaccctgta tcagcgttac cccagaattt ttaagataac
      541 acagctcgtc tttgaccttg ccctcgcgtc gatcgtgatt ttcgtcaccg gcggcaagtc
      601 gagcccgttc atttttcttt acgtgctcat aataatctat tcgagcatag tgctcaacaa
      661 agctgcgagc tatgtaaccg cgctcatttc gggcctcgtc tatgtcctta tcgttttcta
      721 tcaaataagg atcgagacgc cgcttcccga aggacagagt atcctgagct ccgtctccaa
      781 atggggggaa atcgggctgg tctcgacgta ttttcatctg acgggattcc tgcttgttgc
      841 gatcctttcg ggttatctct ccgacaggtt ccgcgccgcg gggagggagc tgggcgagag
      901 tgaaagaagc ctgcgcatcc tccagaacct ccatcagaac atcatcgaga gcctcacgag
      961 cggcatgatt acgctcgatc tcgaaggcag gattatatcc ataaacagga cgggtctcga
     1021 gattctgggt ataaacggcg aggacaggat attgggcaag gacctgggcc agtttatgtc
     1081 cgggctccat ctggaggccc tggtttccaa gaaaagagag cagatgctgt atacggcgcc
     1141 cgacggcagg atggtcactc tcggcttttc cacatcagag ctgaaagaca ccgaagacaa
     1201 gacgcagggg tacataatca tttaccagga ccttactgag gtaaaggagc ttgaggaccg
     1261 gctccggaca tccgagaaaa tggctcttct cggccagctc gccgccggtc tcgctcatga
     1321 gctcaggaac ccgctttcgg ccataagcgg cgcggtcgag atactgggca gcgacgtaaa
     1381 gccgaccgag gagaacctga ggctcgtgcg gatggccacg caggaggtgg agaggttaaa
     1441 cctgctggtc gaggattttc tgattctcac gatgccgatt caaaagctga cgacgcaggt
     1501 cgacctcggc cttatcatca gcgacacggt cgagtctttc acgaagacca tacggcgcgg
     1561 caacatagag attatcaacc aggtcgaaaa gggtatatac gtccaggccg attcataccg
     1621 tttgaagcag gccgtgtgga atctcctgct gaactcggta gacgccatgc ccataggcgg
     1681 cctcataatg ataaaatcca ggaccgaaga taacagcgcg ataatagaga tctcggacga
     1741 aggaaagggt gtggacgaga atttcatatc gaggattttc gagccgtttt ttaccacaaa
     1801 ggaagttgga acgggtctcg gccttgcaat tgtacaaaaa gttatagaag gttataatgg
     1861 aaacattaat gtgataagct cgcgtggcaa cggtgcgaca ttcgtaatca cattgccgag
     1921 gattaaagga gtgcctgccg atgtcataca ctgaaccccc ccggaggtat tgaatggggc
     1981 acaatgacag caacattctc atagtggacg acgaaccggg tatgagggaa ttcctggaga
     2041 tcatgctcca gaaggacggc tattccgtcg atacggcggc tgacggctcc gaagccatag
     2101 acaagatcga gggaagcctg ttcgaccttg ccatcgtcga cattcagatg cccgtgctta
     2161 acgggatcga ggtgctcaag aagtttaacg aaaagagccc agacactact gtaatcatga
     2221 taaccgccta cgcctcccac gaaacggcga tcgaagccat gaagctcggg gcgtacgatt
     2281 atattacaaa gccgttcaag atcgacgaga tcaagctggt cataaaaaag gcgctcgaga
     2341 aaaagaagct cgagcgggag aacaccaggc tcaggaagga gctcgagacg caatacggct
     2401 tcgggaatat tatcggcaga agcccgtcta tcgtaaaagt cttcgagctc attaaaaggg
     2461 tctccgagct gaacgtgagc gtgctcatta ccggagagag cggaacggga aaggagctcg
     2521 tcgcgaaagc catacactat agcgggtcca ggcacgaaaa accgtttgtc ccggtaaact
     2581 gcggggccat tccggaatcc ctcatggaga gcgagctatt cgggtacaag aagggggcct
     2641 tcaccggggc cgtgagggac aagcgagggc ttttcgaaga agctcacggc ggcaccattt
     2701 tcctcgatga gatcggcgat ctgccgcttc atctccaggt gaagctgtta agggtgctcg
     2761 aggagaaaaa gataagaccc ctcgggggta ccgaggccgt gaacatcgac gtgagggtca
     2821 tagctgctac gaataagaag ctcgaggacg aggtcacgag cgggaggttc agggaggacc
     2881 tgttctaccg cctcaacgtc attaaaatag tgcttccgcc cttgagggag agaaagatag
     2941 acgtctctcc gctcgcgata catttcataa acaagtattc ggtcgatatg gagaaggaca
     3001 taagaggcat ttcgccgaag gctctcgaga tactcgagaa ctatcactac ccgggcaacg
     3061 tgagggagct cgagaatatc atagccaggt gcgtcgcgct cgagtcctca aatgtaatcc
     3121 gccaggaaac tcttccgcag ctggtcacgg ggagggatta tctcgacctc gatacgagct
     3181 tcgcctctaa ttccagcctc gacacgctcc tcggggacgt tgaaaagaag atgatagaga
     3241 aggcgctccg caccactaac ggaaacaaga ccgaagccgc gaggctcctg gggataacgc
     3301 tgcgctctct gagatacagg ctcgccaagc acgagctcga cgacgggaac ggagaagacc
     3361 tcgatgatat ggacgaggcg ggggagcttt gagagcaggc cctgggggac tacccgtatt
     3421 tcactgtcac atcttatact tgccgaaatc gccttcaggt atcttgtcca gtatctcggc
     3481 taacttttct ttgtcttcgt ctatttcgat tttcttcgat ttctcgatga cctcatcatt
     3541 gacgtatatg ggggagtcgg tcctgagggc tatggctatg gcgtcgctcg gtctcgaatc
     3601 gagcgagtac tcttcgtttt ctcttttgag gtagatgcgt gcgtagaaag tgtcgtctcg
     3661 gaggtccgtg acctctatct tggtaacaat gaatttcagc ttctcgagga ggtttttgac
     3721 aaggtcgtgc gttatcggcc ttatcctcgg ggttttctcg agctccatgg ctatcgagct
     3781 cgcctccata aaaccgatcc atattgggag tgtcttctcg ttcgccgcgt ccttcaatat
     3841 tacgatagga gtattcgtaa acggatcgag agctatcccc gataccttca tcagaagaaa
     3901 catgagtcac ctcccttttt aattatacag acagttcacc gcgaagggaa ttaaatagtc
     3961 cctctgttat cctgaccttg attattttcc ccgatagccc gctatcacct ttaaaatggg
     4021 tgatccggtt gtggctcgtc ctgcccgtga ggaccccggg gtcgtttttg ctcgctccct
     4081 ccgcaagcac ttcttctatt tgtccgactc ttgcgcggtt ctttctgagt gtaacatccc
     4141 gctggaacga ctgaagcatg gagagtcttc tgcccgcggt gtcctcgtcc acgacctcgt
     4201 ccccgggaca agcggccgcg ggtgtgcccg ggcgcgggga gaatttaaat gagaaagcgc
     4261 tctcgaattc gatctcttcg agcagcgaca tagtatccga gaagtcccgc tcggtctcgc
     4321 ccggataccc gactattatg tccgtggaaa ccgccatgtc cggcacagcg tccttaagtc
     4381 tttttaccgt atccgagtac cattccctcg tataggtcct tttcatcctg cccagtacgg
     4441 cgttcgaccc ggactggacc ggaaggtgaa tatgacggca tatcttaggc acgtccctca
     4501 ccgcctcgat catcctcgcc gttatgtccc tcgggtagga cgtcgtgaac ctgatcctgt
     4561 cgagcccctc cacgtcggcc gcggctctaa ggaggtctcc gaatttatag ccgttctctt
     4621 tccatgaatt cacggtttgg ccgatgaacg taacctcgca cacgccctta tccacgaggc
     4681 tcctcgtctc cctcaatata tcctcgagcg gccggttgac ctcgttgccc ctcacgtaag
     4741 gcactataca gtaagagcac ctcttgttgc atccctgctg caccgaaacg aatgcggtga
     4801 ccttcccttc actgtgatac ggctctatat cgaagagctc ctggatgtcg taggaggttt
     4861 caactgccct gtgcttctct ctctcgatct tttttatgag ctccggcagc ctgggaatgg
     4921 ccctcgggcc cagaacgaaa
//
LOCUS       DASSJH010000142        11295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_248010, whole genome shotgun sequence.
ACCESSION   DASSJH010000142 DASSJH010000000
VERSION     DASSJH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248010"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFJ67_03055"
     CDS             <1..305
                     /locus_tag="VFJ67_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289392.1"
                     /translation="TLKPDSPNANSTSGSPIFPVFAKSAGGRYTFTGSLNALIITKHI
                     TNATPMIMQTDKKTLPARNISIVFFESDDIMSAGVARYITKSLIGERSIFFHLPKK"
     gene            complement(518..1495)
                     /locus_tag="VFJ67_03060"
     CDS             complement(518..1495)
                     /locus_tag="VFJ67_03060"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET7289393.1"
                     /translation="MSYVRFLGTGSSAPEKIMTNLDLEKLVDTSDEWIQTRTGIRERR
                     IAEPGVATSDIAYEAALKALESAGVDARDLDGIIVGTVTPDYLFPSTACILQSRLGAK
                     KAFAFDLLAGCSGFLYALQAGKGMINGGDAKKLLIIGAEVLSKITDFEDRGTCILFGD
                     GAGAAVISESDSPGILSTCLGANGDEWELLYMPGGGSRIPPSEESIRNKSHYLKMKGK
                     EVFKEAVKALESSSLEAIRRADITPEEIDLFIPHQANYRILDAVRRRVGLPEEKVFSN
                     LDRYGNTSSASVPLALDEAVRSGRVKEGDTILISVFGAGFTWGAAVVRW"
     gene            complement(1559..1972)
                     /locus_tag="VFJ67_03065"
     CDS             complement(1559..1972)
                     /locus_tag="VFJ67_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289394.1"
                     /translation="MPELHPDTELSLSLIDSGCLFAQINGKTALIIKGNGDDFSIIKE
                     FQTITIDFELVEKAEFPSLAFFLNIETRSGRRFRFEYFFSMESEDETDILGKMCGDKR
                     FDIILYGSGVDFVIRAEIPEGQAGELRSLLSKAGR"
     gene            complement(2407..2565)
                     /locus_tag="VFJ67_03070"
     CDS             complement(2407..2565)
                     /locus_tag="VFJ67_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289395.1"
                     /translation="MKGSTSPEDCGAGADPPPLDSTADLNGSPPPDEAAGGGGGGGGG
                     GAALAAAA"
     gene            complement(2660..3661)
                     /locus_tag="VFJ67_03075"
     CDS             complement(2660..3661)
                     /locus_tag="VFJ67_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7289396.1"
                     /translation="MPAKNKFIIALGLLLLLWAIVAAVSLLSGTYEHNLIRALFSGDE
                     LAGTIFYKIRIPRVLMATVAGGTLAICGAALQALFRNPLASPFTLGISGGASLGALIG
                     IRLGLAAGILGFSMVTVLAFLFSLLTIIFVYTISRVGGVVATGRLLLAGVVMNFLYSA
                     FVLFIQFFSNFTESLQTMRWIMGSLDIVGFDEVWKTLAFVIPGCVILLSMTKDMNLFG
                     LGDDVASSLGVNVKRMQKLIYFATSLSAGAVISVTGPIGFVGLVIPHILRMVLGVDNR
                     IILPCSFVLGASFLMAADTVSRTLISPIEIPVGIITASLGGIFFLWLLIRTKKEVIV"
     gene            complement(3701..4474)
                     /locus_tag="VFJ67_03080"
     CDS             complement(3701..4474)
                     /locus_tag="VFJ67_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7289397.1"
                     /translation="MGTTIECRGVSFSYGRGEILRGIDLALDGGRMLGILGANGAGKS
                     TLLKILTGILRAQKGSVLFNGRPLQDYDRRELAKKIAYIPQDPVFAFPFTVSEVILMG
                     RAPYIGRFEFEREMDMEAAERAMDTVGITHLKDRLITETSSGERQLASIARALVQEPE
                     MMILDEPATFLDIKHRNEIMNLLRKLRDEHGILIIAATHDIFTALFYFDEIIMIKNGT
                     VFADGSTETVINRENLSSLYGIDVTVRREGGKVFIYPGE"
     gene            complement(4458..5339)
                     /locus_tag="VFJ67_03085"
     CDS             complement(4458..5339)
                     /locus_tag="VFJ67_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HET7289398.1"
                     /translation="MIKRAYSVIMLLALLSASLQSASAGEKPERIVSLSPNLTEIISA
                     LGDLDKIVGVTLYTDSPPRAKELPKVGGWINPNYEAILALRPDLVILMKDQDTIFGAQ
                     IRKLGLKTLVTDSNDSASDIMKTIKYIGEILGKKTEAQRIVSDMQTTLDKIRREAAGV
                     PRKKVLLVVGRNPGTLEDIYVIGRNNYINELIEIAGGSNAVENGRLSIKITKEAILTL
                     DPDVIIEINHEKLDKEAEIIGTWRTLTQSRAVRNGEVYILPSTVVLHPSQTIVEGARV
                     LTDVLHPELKDKYGNNN"
     gene            complement(5343..6290)
                     /locus_tag="VFJ67_03090"
     CDS             complement(5343..6290)
                     /locus_tag="VFJ67_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HET7289399.1"
                     /translation="MRICSFLPSTTEILYGLGLGDSVTGVTHECDYPPEARGKKHVIL
                     SFIEPGKLSSREIDEIISRNVAEGKSTYLIDRDALREADPDLILTQELCQVCAVSGNE
                     VQDAVKVLGKKPEIIALEPRTIGEILETIIVIGEATGTAERAREITQRLAQRIEKVKY
                     NIGDVMDRPRVFCLEWLDPPYAAGHWVPEMVEIAGGRMGLGKAGEPSFKVTWKEIAEF
                     APQMLFIMPCGYDIEKTLGEIDTAMKNDEWFSLPATSKGHIYVFDANSYFSRPGPRVV
                     DGLEILAKTIHPEAMRSYEPPPDSVVNLRNYMQFELFLG"
     gene            complement(6389..7888)
                     /locus_tag="VFJ67_03095"
     CDS             complement(6389..7888)
                     /locus_tag="VFJ67_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289400.1"
                     /translation="MKTLVSFLLFALASLALVSCNDTEGGGGFVPTGTILAVAQKAGS
                     DVTIKVGGLPGAVPPGSTVDVTDIDTGETQSATASSDGSFDPTFTGDTDDEFNVVVTD
                     GGEVVEDIVIGVTLLSDSVEENLAQLGTVPADIQIRGNRAYVVNGFSDNIQIFDLNEN
                     PPGLIGTIVVPPGSNPIDIAFVDDTHAYVVNNIGQSVARVNVNTRICEVLIVKAGQGG
                     ATAPCQSVITVAGTPFEEPVKGIVTKGRLYVTNNNLDANFNPMGNGFISVINATTSQF
                     ITTIPATGTNTTSMVVIGDTLYAMNNGDIIFDPDTNEFTCDFDFPPSIDLIDAQSNVL
                     FDTIDIPLSPENPTVCLPNPLVATPDGRFGYTGLGLVGALLKIDLISGDVINGTDDPI
                     VLTDLDSLNLTADIAIRDNLLFATLFLDDEIAVVDTDTDEVNPFPYIAPFPAGIRAFD
                     PNSEFFDGVQSLAIRPGTPGVNFSGPDIFFITGISEQLGSVDSTLGLAE"
     gene            complement(7934..9814)
                     /locus_tag="VFJ67_03100"
     CDS             complement(7934..9814)
                     /locus_tag="VFJ67_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7289401.1"
                     /translation="MLKTFLSVLLVLLAVPAYAQDDKGETLEEVVVEDTPIEPPLDYP
                     SAFSTVIDLDDFAGEYSTASEILSFSPGVVVRDYGGFGQLKTMSIRGSSNDQVVILLD
                     GMRLNSPIGGGVDLSTIPVNYVDRFEIVRGGASALAGTDAIGGVVNIVTKKPEKPFTF
                     GSVTYGSYETFGFNASSAETLGNFSYLLSFTHAQSKGDFKFKSVNDLTLTRINNEFHS
                     ESLLAKAAYDAGNGWQIEMLNEFFYDDRGVPGLGEFQQPDANQKDLRNLTGINISKEK
                     FMRPDMDLDVLIYNRFDDLKYTNPEPTVGIPIDTLSRTYSFGINPKLTWFGPYEQVFT
                     FATELREEFLESDDYGNPDRFTFSLFAADEINLLDDKILINPVIRYDLWRTSQESTTT
                     DSGVSARLGVIVAPLSYLSFKANAGRSYRAPSFGELFFPNEGFIRGNPDLKPETAYDF
                     DLGFVLSHPRASLEVTYFRSHIDDLILFVFISAQTIEPQNVGNVNEQGIETSLVLKPF
                     RHFELFAGYTFLDGEINETGAQLPGRPRNKLDLRAVLDYGYMSLFWETHYVDRIPVSA
                     FPDSKTTPARTTYDVGLKGEWKKFFVTFEIKNLFNNLDVRDALDFPLPGRTYFATAGM
                     NF"
     regulatory      complement(9895..10080)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(10189..11211)
                     /locus_tag="VFJ67_03105"
     CDS             complement(10189..11211)
                     /locus_tag="VFJ67_03105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7289402.1"
                     /translation="MKFGIGLFGMQTHQELPYTHPDIYRNTLEQVKLAEKGNFDSAWI
                     SEHHFLDDGYCPSPAVMAAAMAAVTTKIRIGSAGIILPLHNPLKVAEDAAVVDNISNG
                     RFDLGVVLGYRKEEYEGMGVPMKQRPSRMDEGIEVLEKALGGGKFSYEGKRYRFENAK
                     LTPGPVQKPIPLYVGAFEEPALRRAGRFGYPLLIGPGRTVEMARDTLGFYNDEARKSG
                     RDPNNVEHILLRETFVSSIRKKAVEGGNKYIINMYKFYFSLGVKMFVRGKQLSGLEDP
                     LFEHLAEDRFIIGTPGDCVDEIEKYRDELGIKYIVCRMVFPQAPHEVISGLIKTFGRK
                     VIPNVN"
BASE COUNT         2345 a         3217 c         2942 g         2791 t
ORIGIN      
        1 ccacgttaaa gcccgactca ccgaacgcga acagcacgag cggaagcccc atattcccgg
       61 tgtttgcgaa gagcgcgggt ggcagatata ccttcacggg gagcctcaac gcccttatta
      121 tcacgaagca tataacgaac gctacgccta tgattatgca gacggataag aagaccttac
      181 ctgcgagaaa tatctctatc gtgtttttcg agagcgatga tattatgagt gccggggtgg
      241 cgaggtatat tacgaagtcg cttatcggag agaggtcaat ctttttccat ctcccgaaaa
      301 aatagcctac aagcgcaatt aggaatacgg gcaggactac gtcggttatc tggctaatta
      361 tggctcttca tctcctgtga aacggggaac gttcatggga acggagctta ttttacgctg
      421 gaataaccgg atagacaaaa acggtatgct caataggtat tgcaaggtat tgcaggaggt
      481 attgcaggag aattcacaaa cggttttatg accgcgccta ccacctgacg actgctgcgc
      541 cccatgtaaa accggcgccg aagacggaaa taaggatcgt gtccccttcc ttcaccctcc
      601 ccgacctcac ggcttcgtcg agcgcgagag gaacggacgc ggacgacgtg ttgccgtacc
      661 tgtcgaggtt gctgaacacc ttctcctccg gcagccccac gcgccttctc accgcgtcga
      721 gtatcctgta attcgcctgg tggggtatga acagatctat ctcctcggga gtgatgtcgg
      781 cccttcttat agcttcgagt gaggaggact cgagagcttt cacggcctct ttgaatacct
      841 cttttccctt catcttgagg tagtgggatt tattccttat gctctcttcg ctcggcggta
      901 tccgcgatcc gccccccggc atgtaaagga gctcccactc gtccccgttc gcaccgaggc
      961 acgtagacaa tatcccgggc gaatccgatt ccgaaatgac cgccgccccc gctccgtccc
     1021 cgaagaggat gcatgtgccc ctgtcctcga aatcagtgat cttcgagagg acctcggccc
     1081 cgattatgag gagctttttc gcatctcctc cattaatcat cccttttccg gcctgaagcg
     1141 catatagaaa tcccgagcag ccggccagaa ggtcgaaagc gaaggctttt ttggctccta
     1201 gacggctctg gagtatgcag gctgtcgagg gaaagaggta atcgggagtg acagtcccca
     1261 cgatgatccc gtccaggtct cgggcgtcaa cacccgcgct ctcgagggcc ttgagcgcgg
     1321 cctcgtaggc gatatcggac gtcgccacac cgggctcggc tattcttctc tctcgtattc
     1381 ccgtcctcgt ctgtatccat tcgtccgagg tatcgacgag cttctcgagg tcgagattgg
     1441 tcattatttt ctcaggcgcc gatgagccgg tgcctaaaaa cctaacataa ctcaaattta
     1501 tgcctctcct ttaccgcttg agggggaata gtatcaggta atcggcatgg ttgtcaaact
     1561 aacgtcctgc cttgctcagc agcgatctca attccccggc ctgtccctcc ggaatctccg
     1621 cgcgtataac gaaatcgacc cccgagccgt ataaaataat gtcgaaacgc ttgtcgccac
     1681 acatctttcc gagtatgtcc gtttcgtcct ccgattccat gctgaagaaa tattcgaacc
     1741 tgaaccttct cccgctccgg gtttctatgt tcagaaaaaa tgcgagcgag gggaattcgg
     1801 ccttttcgac gagctcgaaa tcgattgtaa tcgtctggaa ttctttaatt atgctgaaat
     1861 catccccgtt tcccttgatt ataagagccg tctttccgtt tatttgagcg aaaaggcatc
     1921 ccgaatcgat gagcgatagg gagagctccg tatccggatg gagctccggc atatcccccc
     1981 cgcccttatt tttacccaga aacggtttca gaagaataga taacaattcg ccctcactat
     2041 gttccgttaa ttctattgta ttcggggata ttagccaagc agacgccgat tcggagagtc
     2101 cgccttagaa acccggcgtt tccttcccat ttgcagtccc gtgcaaattt tttatcttga
     2161 caggggcata taaaatttat tagtatcaga aggggtgggt ggttatgccg gtttataaaa
     2221 tgtgagatag gaatccggtt cacacgtcat tcacagtacc ggggtattca attaagtacg
     2281 tgtgtttcta tgaatgcaat ttgaaaccta cataccttga cacggaggaa agttagaaat
     2341 gatgtggaca ttgattgtca taggtttggc ttttgtaatc tggcgcttca tgcagccggg
     2401 tgggagtcag gctgcggcag ccagcgctgc gcctccccct ccaccgcctc ccccgccgcc
     2461 cgcggcttca tccggcggag gcgatccgtt taagtcggcg gtcgagtcca agggcggagg
     2521 gtccgctccc gctccgcagt cttcgggtga ggtcgacccc ttcaaggctc ccggcgcata
     2581 atccggaggc agcagggctt catcacgccc ccgcgtatcc gtattcatcc atactaagga
     2641 acggtgcggt gggggttcgt cagacgatca cttctttctt tgttcttatc aacagccaga
     2701 ggaagaaaat cccgcccaga gaggctgtaa ttatccctac tgggatctcg atgggggata
     2761 tcagtgttct ggatacggta tcggcggcca tgaggaacga cgcgcctaag acgaacgagc
     2821 acggtagaat aatcctgtta tcgaccccga ggaccattct gagaatgtga ggtatgacca
     2881 ggccgacgaa ccctatcggc cccgtcacgg atattacggc tcccgctgaa agggaagttg
     2941 cgaaatagat cagtttctgc attctcttta cgttgacccc gagcgaggac gcaacgtcgt
     3001 cccccagacc gaatagattc atgtccttcg tcatcgacag cagaatcacg cacccgggaa
     3061 ttacgaatgc gagtgtcttc cagacctcgt cgaaccccac aatgtcgagg ctccccatta
     3121 tccacctcat cgtctgaagc gactctgtga agtttgaaaa gaactgtatg aacaggacga
     3181 aggcggaata taggaaattc atcaccaccc cggccagcag cagacgcccc gtagcgacca
     3241 cccctcccac cctggaaatc gtgtagacga atatgatagt caggagagaa aaaaggaacg
     3301 ccagcaccgt gaccatcgag aacccgagaa tgccggcagc gaggccgagc cttattccga
     3361 tgagcgctcc tagggaggct ccgcccgaaa tgccgagggt gaaaggagag gcgagagggt
     3421 tcctgaacag cgcctgaagc gcagcgccgc atatggcgag agtccctccc gccacagtcg
     3481 ccattaacac cctgggaatc ctgatcttgt agaagatcgt ccccgcgagc tcgtccccgc
     3541 tgaatagcgc acggatgagg ttgtgctcgt acgtccccga gagcaggctt acagcggcta
     3601 caatcgccca gagaaggagc aaaaggccga gtgcaataat gaacttattc tttgccggca
     3661 tgtttaatac ttgtgagctt ggatttattc ccatcccgga tcattccccg gggtatatga
     3721 agacctttcc gccctccctc ctgacggtta cgtctattcc gtagagcgag ctgaggtttt
     3781 ccctgtttat taccgtctcc gtgcttccgt ccgcgaatac ggtcccgttc ttgatcatta
     3841 ttatctcgtc gaaataaaag agcgccgtga atatatcgtg cgtcgccgcg attatgagta
     3901 tcccgtgctc gtccctgagc ttcctgagaa ggttcataat ctcgttcctg tgttttatgt
     3961 cgaggaaggt cgcgggctca tcgagtatca tcatttcggg ctcctgcacg agagcccttg
     4021 caatcgaggc gagctgcctt tccccggaag aggtctccgt gatgagccta tctttcagat
     4081 gtgttatgcc gaccgtatcc atcgccctct ctgcagcctc catatccatc tccctttcga
     4141 attcgaacct gcctatgtac ggggccctgc ccataagtat cacttcggat acggtgaacg
     4201 ggaacgcgaa tacggggtcc tgcgggatat acgcgatctt cttggccagc tctctcctgt
     4261 cgtagtcctg gagcggcctg ccgttgaaaa ggacgctccc tttctgcgcc ctgagaatac
     4321 ccgtgaggat tttaaggagg gtcgatttgc ccgccccgtt cgcgcccagt atcccgagca
     4381 tcctgccccc gtccagggcg aggtcgatgc ctctcagtat ctctcccctg ccgtaggaga
     4441 atgagacccc tctgcactca attgttgttc ccatatttat ccttgagctc gggatggagc
     4501 acgtccgtca gcacacgggc gccctcgact atcgtctggc tagggtggag caccactgtg
     4561 ctcggcagta tgtacacctc tccgttcctc accgccctcg actgagtgag cgtcctccac
     4621 gtacctatta tctcggcttc tttgtcgagc ttttcgtggt ttatctctat gattacgtca
     4681 gggtcgagtg tgagaatggc ctctttcgta atttttatcg agagcctccc gttttcgact
     4741 gcgtttgacc cgcccgcgat ctctataagc tcgtttatgt agttattcct gcctatgacg
     4801 taaatgtctt cgagtgtccc cgggttcctc ccgacgacga gaagcacttt tttcctcggc
     4861 actccagccg cctcccgcct gattttatcg agcgtcgttt gcatgtccga gacaattctc
     4921 tgagcttccg tcttcttacc caatatctct ccgatatatt tgatggtttt cattatatcg
     4981 cttgcagagt cgttgctgtc cgtcaccagc gttttgagac cgagcttcct gatctgagcg
     5041 ccgaatatgg tgtcctggtc tttcatgaga atgacgaggt ccggcctgag cgcgagtatg
     5101 gcctcgtagt tgggattgat ccaccctccc actttgggca gctctttcgc ccggggcggc
     5161 gagtctgtgt agagcgtaac ccccactatc ttatccagat cgccgagcgc ggatatgatt
     5221 tcagtgaggt tcggagagag tgacactatg cgctcgggtt tctccccggc ggaggcggat
     5281 tgaagcgacg ccgacagcaa ggcgaggagc atgattacgg agtaagctct cttgatcatt
     5341 ttctatccaa ggaataattc gaactgcatg tagtttctca gattgactac cgaatcgggc
     5401 ggaggctcat agctcctcat cgcttccggg tggatcgttt tcgcaagtat ctcgagcccg
     5461 tcgacgactc tcggccccgg cctgctgaaa tacgagttcg cgtcgaatac gtagatgtgg
     5521 cccttgctcg tcgcgggcag cgagaaccat tcgtcgttct tcattgccgt atcgatctcg
     5581 ccgagcgttt tttctatatc gtatccgcac ggcattatga agagcatctg cggggcgaat
     5641 tctgcgatct ccttccatgt aaccttgaac gaaggctcgc ctgccttccc cagtcccatg
     5701 cgtcctcccg ctatctcgac catctccggg acccagtgcc cggccgcata gggcgggtcg
     5761 agccattcga ggcaaaatac ccgcgggcgg tccattacgt ctccgatgtt gtactttacc
     5821 ttctctatcc tctgcgcgag cctttgggtt atctcgcgcg ccctttccgc agtgcccgtg
     5881 gcttcgccta tgacgattat cgtctccagt atttcgccga ttgtcctcgg ttcaagcgct
     5941 attatctccg gtttcttacc cagaaccttg acggcgtcct ggacctcgtt tcccgagacc
     6001 gcgcacacct ggcagagctc ctgcgtcaat atgagatccg ggtcggcttc cctgagagcg
     6061 tccctgtcta tcaagtaagt gctcttaccc tcggcgacgt tcctgcttat tatctcgtct
     6121 atctcgcggc tcgaaagttt ccctggctcg ataaagctca ggatgacatg ctttttccct
     6181 ctcgcctccg gcggataatc gcattcgtgt gtcacgcctg tcacgctgtc gccgagtccg
     6241 agcccgtaca gaatctccgt cgtgctcggg aggaatgaac agatacgcat aggctaccgc
     6301 ctccctccgg taattccggg gagaggaaat tgaggccctc cccccgtaag attatcatta
     6361 acggctttag tcatttatgc aaacccagct attcggcgag acccagggtc gaatcgaccg
     6421 agccgagctg ctcacttatt cccgtgataa agaatatgtc cggccctgaa aagttcacgc
     6481 ccggcgtccc cggccttatc gcgagcgact gcacgccgtc aaaaaattcg gagttaggat
     6541 cgaacgccct tatacccgcg gggaaaggag cgatataggg gaacgggttc acttcgtccg
     6601 tatccgtgtc cactacggcg atctcgtcgt cgagaaagag cgtggcgaaa agcagattgt
     6661 cccttatagc tatatcagca gtcaggttga gcgaatcgag gtcggtcaga actatcggat
     6721 cgtcagtgcc gtttatcaca tcgcccgaga tcaggtcgat cttgaggagc gcgcccacga
     6781 gcccgagccc cgtatagccg aacctcccgt cgggagtcgc gacgaggggg ttaggaagac
     6841 atacggtcgg gttctccggg ctgagcggta tgtctatcgt atcgaataac acgttcgact
     6901 gagcatctat gagatcgatc gaaggcggga aatcgaagtc gcacgtgaac tcgttcgtgt
     6961 cggggtcgaa gattatgtcg ccgttattca tcgcgtagag cgtgtcccct atgacgacca
     7021 tgctggtcgt atttgtgccg gtggccggga tagtcgttat gaactggctc gtagtggcgt
     7081 tgatgacgga gatgaacccg tttcccatcg gattgaagtt tgcgtccaga ttgttgttcg
     7141 tcacgtagag ccttcccttg gtcacgatcc cctttacagg ttcctcaaac ggcgttcccg
     7201 caacggtgat tacgctctgg cagggcgcgg tagctcctcc ctgtccagct ttcactatca
     7261 gcacctcgca gatacgcgtg ttaacgttta cccgcgcgac gctctgcccg atgttgttca
     7321 ccacgtaggc gtgggtatcg tccacgaacg ctatgtctat cgggttcgat ccgggcggga
     7381 ccacgatagt gccgatgagg ccgggagggt tctcgttcag atcgaatatc tggatgttgt
     7441 cggagaaccc gttcactacg tacgccctgt ttccccttat ctgaatatcg gcgggaacgg
     7501 ttccgagctg cgcgaggttt tcctcgaccg agtcggagag gagcgtaact ccgatgacga
     7561 tatcttccac gacctcgccg ccgtcggtca cgaccacgtt gaactcgtcg tccgtgtcgc
     7621 ccgtgaacgt cgggtcgaag ctcccgtcgg aagacgcagt cgccgattgt gtctcgcccg
     7681 tgtcgatgtc agttacatcc acagtgctcc ccggaggcac tgctcccgga agtcccccga
     7741 cctttatcgt tacgtcggag cccgctttct gcgcgacggc gagtatggtg cccgtcggaa
     7801 cgaatccgcc gccgccctcc gtatcgttgc acgatacgag cgcgagagag gccaaagcga
     7861 ataataggaa agatactaat gtcttcatat ctgagtcctc ctgtgatggt tgttcatctg
     7921 tttttagtct cgttcagaaa ttcattcccg ctgtcgcgaa gtaagtcctc cccggcagag
     7981 gaaaatcgag cgcgtccctc acgtcgaggt tattgaagag gttctttatc tcgaacgtga
     8041 cgaagaattt tttccactcg ccttttaagc ccacgtcgta cgtggtccgg gcgggcgtcg
     8101 tcttcgaatc ggggaatgcg ctcacgggga tcctgtccac gtagtgcgtc tcccagaaaa
     8161 gcgacatgta accatagtcg agcactgccc tgaggtcgag cttgttgcgc ggccttccgg
     8221 ggagctgcgc cccggtttcg tttatctccc cgtcgaggaa cgtgtatccg gcgaagagct
     8281 cgaagtgcct aaacggcttc agcaccaggc tcgtctctat cccctgctcg ttcacgttcc
     8341 cgacgttctg gggctctatc gtctgcgcgc ttatgaagac gaacagtatc aggtcgtcta
     8401 tgtggctcct gaaatatgtc acttcgagag aggcgcgcgg gtgcgagagc acgaacccta
     8461 agtcgaagtc gtaggccgtt tcgggcttga ggtcggggtt tcccctgatg aacccttcgt
     8521 tcgggaagaa tagctccccg aagctcggag cccggtagga gcgtcccgcg tttgccttga
     8581 acgacaggta actgagggga gccacgatca cgcccagccg tgcggatacc ccggaatcag
     8641 tcgtcgtcga ctcctgactc gttctccaga ggtcgtacct gatgacgggg tttatcaata
     8701 tcttgtcgtc gagcaggttt atttcgtcag ccgcgaacag gctgaacgta aacctgtccg
     8761 ggttcccgta gtcgtcgctt tcgagaaatt cctccctcag ctctgtggcg aatgtgaaaa
     8821 cctgctcgta agggccgaac catgtgagct tcgggttaat tccgaacgaa taagtcctgc
     8881 tgagcgtgtc tatgggaatg cccacagtag gctccgggtt agtatatttg aggtcgtcga
     8941 acctgttgta aatgagcacg tcgaggtcca tatcgggcct catgaatttt tccttggata
     9001 tgtttatgcc ggtgaggttc ctgaggtctt tctggttggc gtccggctgc tggaattccc
     9061 ccagccccgg tacccccctg tcgtcgtaga agaattcgtt gagcatttct atctgccagc
     9121 cgttccccgc gtcgtacgcg gctttcgcga gaaggctttc ggagtggaac tcgttgttga
     9181 tcctcgtgag agtgagatcg ttgaccgatt tgaacttgaa gtcccctttg ctctgggcgt
     9241 gcgtgaagga aaggaggtag ctgaagttcc cgagggtttc cgcactcgat gcgttaaagc
     9301 caaaggtttc ataagagccg taggttaccg agccgaatgt aaacggcttt tccggttttt
     9361 tagtaacaat gttcacgacg ccgcctatcg cgtcagtgcc cgcgagagcc gaggccccgc
     9421 cccgtacgat ctcgaacctg tccacgtaat tcacgggtat tgtggagaga tcgacccctc
     9481 cgccgattgg gctattgagc ctcataccgt ctagaagtat cacgacctgg tcgttcgagg
     9541 agccccttat cgacatggtc tttaactgtc cgaacccacc gtagtcgcgg acgacgactc
     9601 ccggagagaa cgaaaggatt tccgaggctg tgctgtactc gcctgcgaaa tcgtcgagat
     9661 cgatgacggt cgaaaaagcc gacgggtagt cgagcggcgg ctctatcgga gtgtcctcga
     9721 ctactacctc ttcgagggtc tctcccttat cgtcctgggc gtaagccggt acggctaata
     9781 gtacgagaag tacggacagg aatgtcttta acacttgagc ccccggttcg cgaacctgaa
     9841 accgggaggc tgttcgggct gaaaacccat ttactttctc ccctcagaag aaaagtaaat
     9901 tcacagttat aaggcagata ttctggctcg tagcctcggg actcccaagc gccttcccgg
     9961 agtgactcct ccagtggcat attgcaaggg actttaagga ctactcacag cagcggggct
    10021 gcaccggatt ctcaccggtt tcccttttaa gctgaaagca ccttatacct ataaattttt
    10081 gaaagagctt taattcatga acatcataac tttctgcata tctaaattca agtgcagttt
    10141 gaatcaatct gatgaatccc gtaatttcac ccacaaacgc gtcccgtttc aattcacatt
    10201 cggaatgacc ttcctgccga acgtttttat caaccccgat atcacctcgt gcggggcctg
    10261 tgggaaaacc atccggcaga ctatgtattt tattccgagt tcgtccctgt acttttctat
    10321 ctcgtcgacg cagtctcccg gcgtgccgat tataaaacgg tcctcggcca ggtgctcgaa
    10381 gagagggtct tctaaccccg agagctgctt tccccggacg aacatcttca cgccgagaga
    10441 gaaatagaac ttgtacatgt tgataatgta tttgttgccc ccttcgaccg ctttcttgcg
    10501 gatagacgac acgaacgtct cccgcagaag tatgtgttcg acattattcg ggtctctgcc
    10561 ggatttccga gcctcgtcgt tatagaatcc gagcgtatcc ctcgccatct ctaccgtcct
    10621 tcccggccct atgagaagcg gatatccgaa ccttcccgcg cgccttagag cgggctcctc
    10681 gaatgcgcct acgtataggg gaatgggctt ctgtacgggc ccgggcgtga gcttcgcgtt
    10741 ctcgaatctg taccttttgc cctcgtagct gaatttcccg cccccgagcg ccttctccag
    10801 cacctctatc ccctcgtcca ttctcgaggg cctttgcttc ataggcacgc ccatcccctc
    10861 atactcctcc ttcctgtacc cgagcacgac gcccaagtcg aacctcccgt tagagatgtt
    10921 atcgacgacg gccgcgtcct cggccacctt caggggattg tggaggggca gtatgatgcc
    10981 ggccgagcca atcctgatct tcgtcgtgac cgctgccatc gcagccgcca ttactgcggg
    11041 cgaagggcag tacccgtcgt cgagaaaatg gtgctcggat atccaagcgg aatcgaaatt
    11101 tcccttctct gccagcttca cctgctcgag agtgttcctg tagatatccg ggtgcgtgta
    11161 aggcagctcc tgatgggtct gcatcccgaa tagtccgatg ccgaatttca tgggtaggtc
    11221 ctccttaaac agacgagtaa gggtctatta taacatccgc ggtcctttta attccaagat
    11281 cacccgctat tattt
//
LOCUS       DASSJH010000143         4696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1438194, whole genome shotgun sequence.
ACCESSION   DASSJH010000143 DASSJH010000000
VERSION     DASSJH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438194"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFJ67_03110"
     CDS             <1..481
                     /locus_tag="VFJ67_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289403.1"
                     /translation="AVGLPQISCDDDGVGDCFGVNLEQSGCVAEDILAIGQEDGLACF
                     NCTGQETGDVFTLTWQKGLLEGTPNPGTIFSFLNEPELWIAEFTDCGTLTLFETFQNE
                     FGRFAKGDLAGTLEDLDAFQVDRLSLFIDIPGVRVEEAVCDFCANSTPPQCFDVVID"
     gene            complement(494..871)
                     /locus_tag="VFJ67_03115"
     CDS             complement(494..871)
                     /locus_tag="VFJ67_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289404.1"
                     /translation="MSRKKAVALALSFGVIISGLYAFRYLNGTHTEYCPLSHKLSDTS
                     DCIVLQISHGRVLVRHRDSFTKEAYFEIIGNGRSRKFEIPDYTIHGAGPKKFRVRLID
                     NEESAVTINGERFELRPITDGDG"
     gene            complement(1120..1686)
                     /locus_tag="VFJ67_03120"
     CDS             complement(1120..1686)
                     /locus_tag="VFJ67_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7289405.1"
                     /translation="MKRAKVIFVFALALAAGCVSQSTYDQQVAETQQLAYLNSVYQQL
                     NTVLAAQVAADQVQIQQLQDQLQVTLVNEILFNEGGWELHAQGRQTLNQIVSSLQQAQ
                     GKYIVIQGYTDNLPILEPLAGLFPTNWDLAAGRAISVVRYLADQGIDPDYLAAVSFGQ
                     YHPVADNSTPEGRAQNRRIEIDIQDQMP"
     gene            1863..3731
                     /gene="pafA"
                     /locus_tag="VFJ67_03125"
     CDS             1863..3731
                     /gene="pafA"
                     /locus_tag="VFJ67_03125"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF042991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019203 - carbohydrate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PafA"
                     /protein_id="HET7289406.1"
                     /translation="MKHSKKSLTVCALILFLTSFFFFSCRQPGPEKPRETPALTAATP
                     SPAPTSAPAPETGPEGNTMPRLVVGIVVDQMAYDFLMRYRDKYGDDGFERLLGDGFSF
                     GNAKFDYVPTTTCPGHASIYTGAPPSVHGIIGNDWFVRAPENDETYCARDVSASLVGD
                     GGGSDDKSGELSPVNMLTSTVTDELRLATGMKSKAIGIALKDRAAIMPAGHLGSAAYW
                     LDDESGEWVTSTYYYRGAKLKEGEKPALPEWVREFNEKYQAEKYLNTPGIDGVWKTLR
                     DISDYTESLPDDSAYEKSIRGKDEKTGEYRKPVFPYVFKDVLAENPEETKGQYGRLIK
                     ATPYGDTLTGDFAEAAIRGEGLGKGGVTDFLAVSFSSTDIIGHYYGPRSVEVEDAYLR
                     IDLDIADLLKTLDREVGEDNYLVFLTADHGVVDVPLYLKDEGIPAGYFMEDDEVISRL
                     NDYLGKAFRLGGIKFVLAYSNQQVYLDRDLIKNRLGLSLDAVEKEAASFLMTLDGVAD
                     VVTSSELSSTDFVSGARHLVQNGHNNKRSGDLAVIMEPGWIEYKGMYGKKGTTHGSPY
                     DYDTHVPLIFYGWNIKHGRSMRPISITDIAPTVSALLKIPYPNGATGNPLPELFDK"
     gene            complement(3761..4351)
                     /locus_tag="VFJ67_03130"
     CDS             complement(3761..4351)
                     /locus_tag="VFJ67_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289407.1"
                     /translation="MRILYVLIIAGLLVMPEARSFAEGDKVHPKKPEAMETYKSGHGF
                     SIEYPKRWQLVTYMEEYQTLREAEKDAGNYFSIQSYQDDDPRIRGYHYFPADTLKIEA
                     WVYPDYGGSLAELITQTKDITRIDDFSIGGKKAKKVWQKVGEGVDEGDEIYSIYFVDA
                     GKRAIFTCYPVYTSLKNKFEEIVGSFRFDVSASDKK"
     gene            complement(4361..4630)
                     /locus_tag="VFJ67_03135"
     CDS             complement(4361..4630)
                     /locus_tag="VFJ67_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289408.1"
                     /translation="MLRIEIWSKGAIEWSLEGTGDWLQYRQAAIKLRSLFPEADEVEL
                     VLDKDNIKTVPIEDAIGEIQSLGGGNNLMLCDKKYEKMMMFVYSG"
BASE COUNT          988 a         1341 c         1315 g         1052 t
ORIGIN      
        1 tgccgtgggg ctgccgcaga tatcgtgcga cgacgacggc gtcggcgact gcttcggggt
       61 gaatcttgag cagtccggct gcgtcgccga agacatactg gcgatagggc aggaggacgg
      121 gctcgcgtgc tttaactgca cgggtcagga gacgggggat gttttcaccc tgacttggca
      181 aaaaggcctc ctcgaaggca cgcccaaccc cggcaccata ttttccttcc tgaacgagcc
      241 cgagctatgg atagccgaat tcaccgactg cgggacgctc acgcttttcg agacattcca
      301 gaacgaattc ggtaggttcg caaaagggga cctcgccggc actctcgaag acctggatgc
      361 gttccaggta gacaggctct cccttttcat cgacataccg ggcgtgaggg ttgaagaggc
      421 agtctgcgat ttctgcgcaa actccacccc gccgcagtgc ttcgacgtcg ttattgatta
      481 gggatcgcct ccctcacccg tctccgtccg tgatgggccg gagctcgaac ctctcgccgt
      541 ttatcgttac ggcgctctct tcgttatcta tgagcctgac gcgaaacttc ttggggcccg
      601 ctccgtgaat ggtgtaatcg ggtatctcga acttccgcga acggccgttc ccgattatct
      661 cgaaataggc ctctttcgtg aagctgtccc tgtggcgtac gagcaccctg ccgtgcgaga
      721 tctggagcac gatgcagtcg gacgtgtcgc tgagcttgtg ggagagaggg cagtactccg
      781 tatgggtccc gttcaggtaa cggaacgcgt ataaccccga aatgatgacc ccgaacgaga
      841 gagcgagcgc gaccgccttc ttccgcgaca attcccggtt ctgcctctgt ttctgtttgt
      901 atcgccccgg cagggtgagc cgggggaccc cattgatgga agaatataat cgacctgcgg
      961 gatttatcca gtgtggggcc agaggcgagg cggtttcgcg gctggaagcc gctcctacaa
     1021 tatcatgtca ttacgaggag cgaagcgacg cggcaatctc gtctttttct ctgttccccg
     1081 aatttacaga ttgcttcgga gcctttcctg ccggctccct tacggcatct ggtcctgtat
     1141 atcgatctcg atgcgcctgt tttgcgcgcg tccctcgggc gtgctgttgt cggctaccgg
     1201 gtgatactgc ccgaacgaca ctgcagcgag atagtccggg tctatccctt ggtcggcgag
     1261 gtaacgtacg acggaaattg cgcgtcccgc ggcgaggtcc cagttggtgg ggaagagtcc
     1321 cgcgagcggc tcgagtatcg gaaggttatc ggtatatccc tgaatgacta tatatttacc
     1381 ctgggcctgc tgaagcgagg acacgatttg gttgagcgtc tggcgtccct gcgcgtgcag
     1441 ctcccacccg ccctcattga acagtatctc gttcacgagc gttacctgta gctggtcctg
     1501 gagctgctgt atctgcacct ggtctgccgc tacctgtgcc gcgagcacag tattcagctg
     1561 ctgatatacc gaattgagat atgcgagctg ctgagtttcg gcaacctgct gatcgtatgt
     1621 actctgcgac acgcagcccg cggcgagtgc gagcgcgaat acgaatatta cttttgccct
     1681 cttcattttt tcctcctgat gagcgatgtt accccgaatt ataaacccaa aacctttttt
     1741 ataaaagtat gattaggagg attggaatct tcgattacaa tatattgtcg gagtctgatg
     1801 cttaatacgg atttaacttt gacggagtaa gattatcccg acatcatagt aaaaaattag
     1861 aaatgaagca ctcgaaaaaa tccctgactg tttgtgccct tatattattt ctcacgtcct
     1921 ttttcttttt ttcctgcagg cagcccgggc ctgagaagcc gcgcgaaacc ccggccttga
     1981 ctgctgcgac tccgtcgcct gctccaacct cagctcccgc tcccgaaaca ggccccgagg
     2041 gcaacactat gccgaggctc gtcgtgggga tagtcgtcga tcagatggct tacgatttcc
     2101 ttatgagata cagggacaaa tacggggacg acgggttcga gaggctgctc ggcgacggtt
     2161 tctcgttcgg gaacgcgaaa ttcgattacg tgccgaccac cacctgcccc ggccacgcct
     2221 ccatatacac gggcgctccc ccgtccgtgc acggcatcat agggaacgac tggttcgtcc
     2281 gggcccccga gaacgatgag acgtactgcg cgcgtgacgt ctccgcgagc ctcgtagggg
     2341 atggcggggg cagcgacgat aaatccggtg aattgtctcc cgtgaacatg ctcacgtcca
     2401 cagtcaccga cgaactccgg ctcgctaccg gcatgaaatc gaaggcgata gggatcgcgc
     2461 tcaaggacag ggctgccata atgccggcgg gacatctcgg cagcgccgcc tactggctcg
     2521 acgacgagag cggcgaatgg gtcacgagca cgtattatta ccggggggcc aagctgaaag
     2581 agggcgaaaa gcccgcgctc ccggagtggg tcagggagtt taacgaaaaa tatcaggcgg
     2641 agaaatacct taacacaccg ggcatagacg gcgtctggaa aactttgagg gacatatcgg
     2701 actacaccga aagcctcccc gacgacagcg cctacgagaa atcgataaga gggaaggatg
     2761 agaaaacagg ggagtacagg aagcccgtct tcccttacgt attcaaggac gtgctcgccg
     2821 agaacccgga agagacgaag gggcagtacg ggaggctcat caaggcgacg ccctacggag
     2881 atacgcttac cggggacttc gccgaagccg cgataagggg cgagggtctc gggaagggcg
     2941 gcgttacgga ttttctcgcg gtcagctttt catcgaccga cataatcggg cattactacg
     3001 gcccgaggtc ggtcgaggtc gaggacgcat atttgagaat tgacctcgat atagctgacc
     3061 tgctcaagac gctcgaccgc gaggtgggcg aggacaacta cctcgtgttc ctcacggcag
     3121 accacggggt cgtggacgtg ccgctctatc tgaaggacga ggggataccg gccgggtatt
     3181 tcatggaaga cgacgaggtg atttcgcgcc tgaacgatta cctgggaaaa gcgttcaggc
     3241 tcgggggcat aaagttcgtc ctcgcataca gcaatcagca ggtgtacctt gaccgcgacc
     3301 tcataaagaa caggctcggg ttatctctcg atgccgtgga gaaggaggcg gcctccttcc
     3361 tcatgacgct cgacggagtc gcggacgtcg ttacgtcctc ggagctcagc agcaccgatt
     3421 tcgtctcagg cgcgaggcat ctcgtccaga acggacataa caacaagaga tcaggggacc
     3481 tcgccgtgat catggagccc gggtggatcg agtacaaggg gatgtacggc aagaagggga
     3541 cgacacacgg ctcgccttac gactacgaca cgcacgttcc gctcatattt tacggatgga
     3601 acatcaaaca cgggcggtcc atgaggccca tctctataac cgacatagcg cccacggtct
     3661 cggcgctcct caagataccg tacccgaacg gcgcgacagg gaacccgctc cccgaattgt
     3721 tcgataagtg attccggcta tagccagagc tcctgaagcc tcattttttg tccgatgccg
     3781 acacgtcaaa cctgaagctc ccgacaatct cttcgaactt gtttttcagg ctcgtatata
     3841 cggggtagca ggtgaatatc gccctcttgc ccgcgtcaac gaagtatatg gaatagatct
     3901 cgtccccttc gtccacgcct tccccgacct tctgccacac cttcttcgcc ttcttcccgc
     3961 ctatcgagaa gtcgtcgatc ctcgttatgt ccttagtctg ggtgatgagc tccgcgagcg
     4021 agccgccgta gtcgggatag acccatgcct ctatcttgag cgtgtcggcg gggaagtaat
     4081 ggtatcccct tatgcgcggg tcgtcgtcct gataactctg gatcgagaag tagttccccg
     4141 cgtccttctc cgcctccctg agggtctggt attcctccat gtaagtgacg agctgccatc
     4201 ttttggggta ttcgatcgag aaaccgtgcc cgctcttgta cgtctccatg gcctccggtt
     4261 ttttcggatg caccttatct ccctcggcga agctccgtgc ctccggcatg acgagtagtc
     4321 ccgcaatgat gagtacgtac agaatcctca tttttcacgc tcagccgcta tagacgaaca
     4381 tcatcatttt ctcatatttt ttgtcgcaaa gcatcaggtt attccccccg cccagggatt
     4441 gaatctcgcc aatcgcgtcc tctatcggca cggtcttgat gttgtcctta tcgaggacca
     4501 gctcgacctc gtcagcctcc gggaagagcg agcggagctt tatagcggcc tgcctgtact
     4561 ggagccagtc cccggtcccc tcgagagacc actcgatcgc ccccttcgac catatctcta
     4621 ttctcagcat gttatatcag attttaccct gtaatcattg cgtgcttgtg atgcagacgc
     4681 tgcggtgaaa ttcgaa
//
LOCUS       DASSJH010000144         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1219811, whole genome shotgun sequence.
ACCESSION   DASSJH010000144 DASSJH010000000
VERSION     DASSJH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219811"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..575
                     /locus_tag="VFJ67_03140"
     CDS             189..575
                     /locus_tag="VFJ67_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7289409.1"
                     /translation="MININSPVLRMFVTVVLVLMGFNLFMAVSRADEAETGKKLFQER
                     GCLACHTIGKGKLTGPDLAGVTKRRDEEWLRKWLKSPETMLMSDPVAKELLQQYMVPM
                     PNQGLSDQDIEALIDYFEYEDSGKSK"
     gene            611..2650
                     /gene="nosZ"
                     /locus_tag="VFJ67_03145"
     CDS             611..2650
                     /gene="nosZ"
                     /locus_tag="VFJ67_03145"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HET7289410.1"
                     /translation="MTGIKRLIALLPVFLLALTPVCFTSCKNSGEEKEAQKQAADVEM
                     PASVEEIAQERGLSPKDVVAALKTYIPSGKYDDYVMFASGGHSGQVFVIGVPSMRLLK
                     SIAVFTPEPWQGYATGSKESHEILSEGNMPEGSDNKLTWADVHHPALSETNGDYDGQF
                     LFVNDKANARVAVIDLRDFETKQLIKNPNTISDHGASFVTPNTEYVIETTQYSAPLPF
                     DEYAGLDQYKEKYRGLATFHKFDREKGRIDESQSFSIELPPYWQDLCDAGKKVSEGWA
                     FCNSINSEMAIGGINEGNPPFEAGISQKEADYLHVFNWKKAEEVFKAGKAKQLNGQNI
                     IPLDTAIAEGLIYFMTVPKSPHGVDVSPTGNFIVASGKLDTHATVYSFNKIMDAIKNN
                     KIEGKDPYGVPILNFNDVMEKQVEIGLGPLHTQFDDKGNAYTSVFVESTLAKWRLGPP
                     EYDGPDAWTVVEKMPVQYNIGHVVCAEGDTVAPDGKYCVALNKWSIDRFLPVGPLHPQ
                     NFQLIDISGKNMQLLYDMPIGVGEPHYVQMIKADKLKPWLVYPEVGFNPMTMSVDPEA
                     ASVGNERIVRNGDNVEVWMSVIRSHFNPEHIMVNEGDNVTIHLTNIERARDATHGFAI
                     DGYNVNLSLEPGEASTVEFVADKPGVFPFYCTEFCSALHLEMMGYLLVQPKEPKS"
     gene            2749..3144
                     /locus_tag="VFJ67_03150"
     CDS             2749..3144
                     /locus_tag="VFJ67_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289411.1"
                     /translation="MNAIGIKPICAVTVLLLALFIGAGGSYAADQSAWKDSALSWQKQ
                     AKDTRVVVVGVADELGKTGDKGNADVKGLIQDAVTWLGEGDKNMATADEAMTKEDYQK
                     ASNEYNMAWQYYVKAATAGLNAKSILGGL"
     gene            3287..3940
                     /locus_tag="VFJ67_03155"
     CDS             3287..3940
                     /locus_tag="VFJ67_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HET7289412.1"
                     /translation="MKENELIFGPRIPRELWEKERKRFLIPTIVLSAAAVLLFISIFF
                     PYWKITLFAPQYPQGLAASMYVNRLTGDVQEIDGLNHYIGMKPLGEAALLERTLSVFI
                     IIGIALLIAGAAYVHSPFALFLCIPAVFYPAFFLGDLYFWMRSFGMNLDKRAPLSGAI
                     KPFVPPLLGEGKIAQFSTTATWETGLYLSIAASVLILIGLYFHRKAYKPLLVGERSK"
     gene            3970..>4238
                     /locus_tag="VFJ67_03160"
     CDS             3970..>4238
                     /locus_tag="VFJ67_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HET7289413.1"
                     /translation="MTRNILLLISAIAILTLSSASAGLALDKTMRVSPDGPYRTITEA
                     INAASPGDTISVGAGTYRERLVIGKTVRLIGVGNPVIDGGGSGTVV"
BASE COUNT         1078 a         1094 c         1217 g          849 t
ORIGIN      
        1 atacctcata ttcttgtttt gacccgcgtc ctgcaatcgc aaatgactga gtgagcgcgc
       61 actcattttt ttgtactata atcggacaac cgacaagagc ggatttacat taatactgta
      121 attgctgtgt gttacgactg gcatttaact ttcataatac agacggcaaa aggaggtttt
      181 caatatctat gatcaatatt aattcacctg tgctcaggat gtttgtaact gttgttctgg
      241 ttcttatggg gttcaatctg tttatggcgg tttcccgtgc cgatgaggcg gagaccggta
      301 aaaagctgtt tcaggaaagg ggctgcctgg cctgccatac gataggcaag gggaagctca
      361 ccgggcccga cctggccggg gtaacgaaga ggagggacga ggaatggtta aggaagtggc
      421 tcaagtcgcc cgagacgatg ctgatgtccg acccggtggc gaaggagtta ctccagcagt
      481 acatggtgcc gatgcccaac cagggacttt ccgaccagga catcgaagcc cttatcgatt
      541 atttcgaata cgaggattcc ggaaaaagta agtaaataaa tcgttctata cactcgagga
      601 ggtgtgcaaa atgacaggaa ttaaaaggtt aatagcgtta ctgccagttt ttctgctggc
      661 cctgacgcct gtttgtttca cgagctgcaa gaactcaggc gaagaaaagg aggcgcagaa
      721 acaggcggcc gatgtcgaga tgcccgcgag cgtcgaggag atagctcagg agagagggct
      781 ttcgcccaag gacgtcgtgg ctgctctcaa gacgtatata ccttcgggca agtacgacga
      841 ttacgtaatg ttcgcgtcag gcgggcactc cggtcaggta ttcgtgatag gggtcccttc
      901 catgaggctg cttaaatcga ttgcagtctt cacgcccgag ccctggcaag gttacgctac
      961 ggggtcgaag gaatcgcacg agatactttc cgaaggcaac atgccggaag ggtctgacaa
     1021 caagctcacg tgggccgacg tccaccatcc ggcgctcagt gaaacgaacg gggactacga
     1081 cggtcagttc ctcttcgtga acgacaaggc aaacgccagg gtcgccgtaa tcgatctgcg
     1141 cgacttcgaa acgaagcagc ttatcaagaa ccccaatacg atttccgatc acggggcaag
     1201 cttcgtgacc ccgaatacgg agtacgtcat cgaaacgacc cagtattcgg cgcctcttcc
     1261 cttcgacgag tatgcggggt tggaccagta caaggagaag tacaggggac tcgcgacatt
     1321 tcacaagttc gacagggaga agggcagaat agacgaatcc cagtcgttct cgatagagct
     1381 cccgccttac tggcaggacc tctgcgacgc cgggaagaag gtgagcgagg gctgggcctt
     1441 ctgcaactcc atcaattcgg aaatggcgat aggcggaata aacgagggta acccgccttt
     1501 cgaggccggc atatcgcaga aggaagcgga ttacctccac gtattcaact ggaagaaggc
     1561 cgaggaagta tttaaagccg gcaaggccaa gcagctgaac ggtcagaaca tcatcccgct
     1621 cgatacggcc atcgccgaag ggctcatcta cttcatgacg gtaccgaaga gcccgcacgg
     1681 cgttgacgtc tcgccgacag ggaatttcat cgtcgcgtca ggaaagctcg acacgcacgc
     1741 aacggtttat tcgttcaaca agataatgga cgcgatcaag aataacaaaa tcgaaggcaa
     1801 ggacccttac ggcgttccga tactcaactt caacgatgta atggagaaac aggtcgagat
     1861 aggcctcggc cctctccaca cgcagttcga cgacaagggc aacgcctata cgagcgtgtt
     1921 cgtagagagc accctcgcca agtggagact cgggcctccg gaatacgacg gccccgacgc
     1981 atggacggtc gtcgagaaga tgcccgtaca gtacaacata ggccacgtag tgtgcgctga
     2041 aggtgacacg gtcgcgcccg acgggaaata ctgcgtcgcg ctcaataagt ggtcgataga
     2101 caggttccta ccggtagggc cgctccaccc gcagaacttc cagctaatcg atatctcggg
     2161 gaagaacatg cagctccttt acgacatgcc cataggagtg ggcgagcctc actacgtaca
     2221 gatgataaag gcggacaagc tgaaaccgtg gctcgtttac ccggaagtcg gttttaaccc
     2281 gatgaccatg agcgtcgacc ccgaagccgc ctccgtggga aacgagagga tagtgcgcaa
     2341 cggggataac gtcgaagtgt ggatgtccgt gataagaagc cacttcaacc ccgagcacat
     2401 tatggtgaat gaaggcgaca atgtaaccat tcacctcacg aacatagagc gcgcgaggga
     2461 cgcgacgcac ggcttcgcga tagacggcta caacgtgaac ctgagcctcg agccggggga
     2521 agcgagcacg gtcgagttcg ttgcggacaa gccgggggtc ttccccttct actgcacgga
     2581 attctgctct gcgcttcacc tggagatgat gggctatctc ctcgtacaac ccaaagagcc
     2641 caagtcatga gttatggagg gcgactctcc cggatcttta gtcacgggga gtgaaggaag
     2701 ggccgccctc tcttaaaaac gatatccgaa aaggagggta ttgaaaaaat gaacgcgata
     2761 ggaataaaac ccatatgtgc agtaacggtc ttgttgctgg ctcttttcat cggcgcaggc
     2821 ggctcatacg cagccgatca gtccgcctgg aaggacagcg cgctgtcctg gcagaagcag
     2881 gccaaggaca cgagggtggt tgtcgtggga gtcgcggacg agctcggaaa gaccggggac
     2941 aagggtaacg ccgacgtaaa gggcctcata caggacgccg taacatggct cggagagggc
     3001 gacaaaaaca tggctacagc cgacgaagcc atgacgaagg aagactacca aaaggcctcg
     3061 aacgaataca acatggcgtg gcagtactac gtgaaggcag cgaccgcggg ccttaacgcg
     3121 aagagcattc taggcggtct gtaaggtttt attgatattc gctctcgtat gctgtcgggg
     3181 acttctcgtt accaggagaa ttgttcgggg agtccccgtt tataaaggga aagcgtttcg
     3241 aagaaaaatt tgacgtaatg gaatctaata acggaggcat tattaaatga aggaaaatga
     3301 actgatattc gggccgcgca tacccaggga gctctgggaa aaggagagga agaggttcct
     3361 tatcccgacg atcgtgctat cggcagccgc ggtgctgctc ttcatatcca tattcttccc
     3421 gtactggaag ataacgctct tcgccccgca gtacccgcag gggctcgcgg ccagtatgta
     3481 cgtgaacagg ctcacgggag acgtacagga gatagacggt ctcaatcact atataggcat
     3541 gaagcccctg ggcgaagccg ctctactcga gcgcacgctg agcgtattca ttataatcgg
     3601 aatcgccctt ctaattgcgg gcgcggccta cgtacacagc cccttcgcgc ttttcctatg
     3661 cattcccgca gtgttctacc cggcgttttt cctcggagac ttatatttct ggatgcggag
     3721 cttcggcatg aacctcgaca agagggcgcc cttaagcggg gcgataaagc cgttcgtgcc
     3781 gccgctcctg ggcgagggaa agatagccca gttctcgacg acggccacgt gggagacagg
     3841 gctctatttg tctatcgcgg cctccgttct catactcatc ggactatact tccacagaaa
     3901 ggcctacaag ccgctcctag tcggtgaaag gagcaagtag gcgaggtaag aaatatacga
     3961 aggtgacccg tgaccagaaa catccttctc ctgatttccg ctatcgctat tctgacgttg
     4021 tcatcggcct cagccggact cgccctggac aagacgatgc gcgtctcccc ggacgggcct
     4081 tatagaacga tcacggaagc tattaacgca gcctccccag gcgacaccat cagcgtcggg
     4141 gcggggactt acagagagag gctggttata ggcaagacgg tccgacttat aggcgtcggc
     4201 aatcccgtca tagacggcgg gggcagtggg accgtggt
//
LOCUS       DASSJH010000145         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1220022, whole genome shotgun sequence.
ACCESSION   DASSJH010000145 DASSJH010000000
VERSION     DASSJH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220022"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFJ67_03165"
     CDS             complement(<1..844)
                     /locus_tag="VFJ67_03165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET7289414.1"
                     /translation="MNEPVIIDAIRTPVGRNRGALRNITPEELYAGLIGEMLKRTGID
                     GDKIDDVITGCVTQYGEQGANIGRLAVLLSPLPSSVPAVTLNRMCGSSQQAVHFAAQS
                     IAAGDAGYVLAGGVESMTRVPMFSDIKGGLENLNPGIGSKYELIHQGESAERIAEKYG
                     LAREELDRFAFESHTKAARAIMSGFFREQIIPVEGADADGNPFVLVYDEGVRLKPDLE
                     RMSSLSPVFREGGVITAANSSQISDGAALLLIADKERAEADGLSPRARIRARVVLGGD
                     PTMQL"
     gene            complement(841..1977)
                     /locus_tag="VFJ67_03170"
     CDS             complement(841..1977)
                     /locus_tag="VFJ67_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7289415.1"
                     /translation="MDFDFTEEQKMIRSVAREFARKEIAPNAEYYDRSSEFPHEIQRK
                     ARELGLINVIIPEEYGGSGLSAIEMIIVGEELAWGCAGIAVGGIAINTLAAQPILLAG
                     DSEQKKRYLGILSRGYGSYCVSEPNAGSDVASMRATARKLRDKYILSGQKTWISNAGE
                     ASFFIVFAKTDPSEGHRGISAFIVDRDAPGVEVSKKLRKMGQRASDACEVIFNDVELS
                     RNSLLGNEGEGFKLAMKVFDHTRPLIAALGVGVSQRALDECISYSKEREAFGRPILDF
                     QGVSFKIAEMGMRTQAARLLTYNAAWKAARGERNSLEASYAKTFASDTAMWAATEAVQ
                     IHGGYGYSEEYPLEKLMRDAKVLQIYEGTNEIQRVVMAKELTRE"
     gene            2069..>2870
                     /locus_tag="VFJ67_03175"
     CDS             2069..>2870
                     /locus_tag="VFJ67_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7289416.1"
                     /translation="MEKKVIGIDVGGTNLRGALVGADGKITKRMKIASGADLGIETVI
                     KNLVSLIGSISEGEDISAVGFGIPGIIDFNDGVITQAPNISNVNNYPIRQNLRSRIGD
                     EIVVIVENDANCAAVGEWWMGAGKDIDSLVMITLGTGVGGGIILDGKLWRGADGFGGE
                     VGHMTIYPGGPRCNCGNYGCLEVYSSATGIRRMVKETLSDPNTKTALRELVRNEDPGR
                     IPEVVMKAATDGDEAALRIWEQFGTALGIGMASLVNILNVEMIVIGGGV"
BASE COUNT          565 a          802 c          828 g          675 t
ORIGIN      
        1 ggagctgcat tgtcgggtct ccaccgagaa ctacccttgc gcgtatccta gcgcgcgggg
       61 aaaggccgtc cgcttccgct ctttccttgt ccgcgatgag aaggagcgct gccccatccg
      121 atatctggct cgagttcgct gcggtgatca cgcctccttc cctgaatacg ggcgacaatg
      181 aagacatcct ttcgaggtcg ggtttgagtc tcactccttc gtcgtaaacg aggacgaacg
      241 gattcccgtc ggcgtcggca ccctcgacag gtatgatctg ctccctgaag aagcccgaca
      301 tgattgcccg ggcggctttc gtgtggctct cgaaggcgaa cctgtcgagc tcctctctcg
      361 caaggccgta tttctccgct atgcgctctg cagattctcc ctgatgaata agctcatatt
      421 tactgccaat cccagggttc aggttttcga ggccgccttt tatgtccgag aacatcggga
      481 cacgcgtcat tgactcgacg ccccccgcga gcacgtatcc ggcatcaccc gcggctatcg
      541 actgagcggc gaagtggact gcctgctggc tagagccgca cattctgttt agcgttacag
      601 ccgggacgga ggaggggagg ggggagagga gcacggcgag tctcccgata tttgcgccct
      661 gctcgccgta ttgagtcacg cagcccgtga ttacgtcgtc gatcttgtct ccgtcgatgc
      721 ctgtcctctt cagcatctcg cctatgagcc cggcatataa ctcctcgggt gtaatgttcc
      781 taagcgctcc ccggttcctg cctacgggtg tgcgtattgc gtctataatt acaggttcat
      841 tcattctctc gtcagctcct ttgccataac gactcgctgt atctcgttcg tcccctcgta
      901 tatctggagc accttcgcat ccctcatgag cttctctagc ggatattcct ccgaataccc
      961 gtagcccccg tgtatctgga cggcttcggt cgcggcccac atggcggtgt cggaggcgaa
     1021 cgtcttggcg tacgaggcct cgagcgagtt ccgctcgccc cgggcggcct tccacgccgc
     1081 gttatacgtg aggagcctcg ccgcttgcgt acgcatgccc atttcggcaa tcttgaaact
     1141 cacgccctgg aagtccagaa tgggacggcc gaacgcttcc ctttctttcg agtaacttat
     1201 gcattcgtcg agcgcgcgct gggagactcc gacgccgaga gcggcgataa ggggtctcgt
     1261 atgatcgaag accttcatgg cgagcttaaa cccttcgccc tcatttccga ggagcgagtt
     1321 ccgcgaaagc tccacgtcgt taaaaatcac ctcgcacgcg tcgctcgccc tctgtcccat
     1381 ttttctcagt tttttcgaca cctcgacgcc cggggcgtcc ctgtcgacga tgaatgcgct
     1441 tattcccctg tgtccttcgc ttggatcggt tttggcgaac acgatgaaga aagacgcctc
     1501 gcccgcgttc gatatccagg ttttctgacc gctgagtatg tatttgtccc tgagcttgcg
     1561 ggcggtcgcg cgcatggagg cgacgtctga gccggcgtta ggctcggaga cgcagtacga
     1621 tccgtagccg cgggagagta tgccgagata tctttttttc tgttcgctgt ctccggcaag
     1681 cagtatgggc tgcgcggcga gcgtgtttat cgctatgccg ccaaccgcaa tacccgcgca
     1741 tccccatgcg agctcttccc cgactataat catctctatc gcggaaagac cggagccgcc
     1801 gtactcttcc gggataatca cattaatgag tcccagctcg cgcgccttcc gctgaatttc
     1861 gtgcgggaat tcagagctcc tgtcgtagta ttccgcgtta ggggcgatct ccttcctcgc
     1921 gaattccctc gcgacgctcc ttatcatctt ctgctcttcg gtgaagtcga agtccattta
     1981 ttttctctcc ccgtctttac actacccgtg tatggatgat attttacagc atgcatgagc
     2041 acatttttat acgggcggta ctaatgagat ggaaaagaag gttatcggta tagacgtcgg
     2101 aggcactaac ctgaggggag cgctcgtggg cgcggacgga aagattacga agcgtatgaa
     2161 gattgcgtcc ggggccgatc tcggaatcga gactgtcatt aaaaatctgg tcagccttat
     2221 aggaagtatc agcgaggggg aagatatctc ggctgtgggc ttcggtatac cgggaataat
     2281 cgatttcaat gacggtgtta tcacgcaggc gcccaatatc tcgaatgtga acaactatcc
     2341 gatcaggcaa aacctccgca gcaggatagg cgacgagata gtcgttattg tggaaaacga
     2401 cgccaattgc gctgcggtag gggagtggtg gatgggggcc gggaaggata tcgactcgct
     2461 cgtgatgatc acgctcggga cgggagtggg cggcgggata atactcgatg gaaaactttg
     2521 gcggggtgcg gacggattcg gcggagaggt cgggcacatg actatctatc cgggcggccc
     2581 gagatgcaac tgcgggaatt acggatgcct cgaagtctat tcgtcggcga cgggcataag
     2641 gcgcatggtt aaagagacgc tctctgatcc gaatacaaag actgcgctga gggaactcgt
     2701 tagaaatgaa gacccgggca ggattccgga agtcgtcatg aaggctgcta cagacgggga
     2761 cgaagctgct ctccggatat gggagcagtt cgggaccgcg ctcgggatcg ggatggcgag
     2821 tctcgtgaat atacttaacg tcgagatgat cgtaataggc gggggagtat
//
LOCUS       DASSJH010000146         5147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_682224, whole genome shotgun sequence.
ACCESSION   DASSJH010000146 DASSJH010000000
VERSION     DASSJH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682224"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFJ67_03180"
     CDS             complement(<1..536)
                     /locus_tag="VFJ67_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7289417.1"
                     /translation="MAALDNKSVCCSGPAPAEYARGTTVDLFRAAGTVSGELREILAR
                     AEGGESLNREEALLVSGLGREEVPFLLRTASGIRDRGKGKTLTFSRNVFVPLTQLCRN
                     RCGYCTFKYEPGEGPLFLTPEEVIDMAKKGAELGCTELLFVTGDKPELMYPEYREALR
                     KLGYKSTAEYLIAMGEAGL"
     gene            complement(682..1608)
                     /gene="cofD"
                     /locus_tag="VFJ67_03185"
     CDS             complement(682..1608)
                     /gene="cofD"
                     /locus_tag="VFJ67_03185"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HET7289418.1"
                     /translation="MITALGGGVGAAKFLEGLSHVISGEPLSVVVNTGDDIVLHGLRI
                     SPDVDTIIYRLSGFIDTGKGWGVRNDTFTCLAALSRFGAETWFNIGDIDFATHIYRTR
                     LRNEGFTPVQVTERLARAFGIEDSIKVLPMTDEDVETWIITDEGEMHFQEYFVKRKMK
                     PEVLKVVFRNIERAKPAPGVLEAIRDARMIILCPSNPIISIGPILNVKGIGEALRASG
                     GRVIAISPLIGGVPLKGPADKLMRGLGLEVSSAGVARLYRDFLDVMVIDTEDAREAER
                     ISEMGIKPVVLDTVMSDAEKAAALASAVLSEI"
     gene            1884..3491
                     /locus_tag="VFJ67_03190"
     CDS             1884..3491
                     /locus_tag="VFJ67_03190"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET7289419.1"
                     /translation="MSLNFIRNRHSWFIRGILIVIALAFIIGIGYNLSNFGAITNVPD
                     RTAAKVNGEDVSLVNFYIMRDSLKRQIGQEGEVPEEYMNQINVIALNQLINLKLLAQK
                     AKSLGFRVTDEELGSAITTDPNFQIDGKFVGRDRYRQFVEQALHQEVGDFENTYRERL
                     LAEKLARFLDETAIITNENLLEVYNKQNEKVNLYYISFPADDFAGSYTPGEKEIKDYY
                     QKHKGELKTAETRQIRYFTLSPETLEKSIVVSEDEIGSYYTAYPEEFKSQDGKQIPLS
                     DVKGDIESKLKAQKAEALRQQLMARIDNPGEGGPAGIDTLAKEYSVETINESKPFTMT
                     DIMADVPPMVTRQAFSGDKGTVSLIPVGTSIWVMEVTSITPPKEQTFEEAESNITATL
                     KHEKSLELAKSKAEETLKKLRGVKNENLPAETKKLGLQLKETGLFSRSEKVPQLNSPI
                     IGSDAFELDVKAGVPNRIYDDKDKFVIISVKELMSASPGDFDEVRGALMQQELDKQRS
                     QVIQKVIQDMRRQAEIVPNNKLFPAQG"
     gene            3519..>5147
                     /locus_tag="VFJ67_03195"
     CDS             3519..>5147
                     /locus_tag="VFJ67_03195"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET7289420.1"
                     /translation="MSRRALIIGRDRDYCYVLREFLALRELYVTVVLDYKEGLDRLFY
                     EKPDLTIFESSGPEPVRACVDSIRESNEFEIVDFGSGQAIKTGAKPVLLFDDKSRINS
                     LFDFLKSGFSQPQPEQPPDTGDEGSLESTFYPSLLVDINLKKKSGVLSITSSTNLIIY
                     FINGNPVFAEGGDIETAIGRILLNSGKIDEETYEKAVDIASKNKQKFGQILFEMGITS
                     PHELNSFLEFQMQEKILKGFYYTKGKYEFKSGDEFADRIVLYQVDVSKVIYEGVKRYI
                     DVNDLEEANPAIIIDPRLRTDINSLGLKPKELRFVQLLKDRATVVEVLEGSRLERPEA
                     LKLLYFLSLFRLVKMSGISTDDIGRASIEKRIRESETGPGVSGAPEFHSDMESVFAEL
                     KELESEESPEVGLGTGHSDTVDEIVYQGAEQEPEAKSAAGNSHAFESRFGFSEGASEP
                     EVPFEGSPNGGFESPGPSAYEENNKEEEYYILDTEREEPQAGEPQGSPPVYEIELDLD
                     LDPEAGENGQSAWGKAPTTPQFQEPARPGGEDREG"
BASE COUNT         1395 a         1339 c         1387 g         1026 t
ORIGIN      
        1 agacccgcct cccccatagc tatgagatat tcggcagtgc ttttgtatcc gagcttccta
       61 agcgcctcgc ggtactcggg gtacatgagc tcgggcttgt ctccggttac gaagaggagc
      121 tccgtgcagc ctagctcggc ccctttttta gccatatcta tgacttcttc aggcgtgagg
      181 aaaagggggc cctctccggg ctcgtattta aaagtgcagt atccgcagcg gtttctgcaa
      241 agctgggtga gcgggacgaa tacgttcctc gagaacgtaa gcgtctttcc cttccccctg
      301 tctcttatcc cggaagctgt gcggagcagg aagggcacct cttccctgcc cagcccggaa
      361 actaaaagag cttcctcccg gttgagagac tctccgccct cggccctggc gagtatttca
      421 cgcagctcgc cggaaaccgt tccagccgcg cggaacaggt cgaccgtcgt accccgggcg
      481 tattccgcag gggcgggccc ggaacagcaa accgatttgt tatcaagagc ggccattatt
      541 ctcccagata tataattaaa ccagatattt attcagataa aagtctgatt ataacgatgc
      601 acgctgcggt tatcaccccg gtttaaccta ttaatcatta gcacaaacgg gtaaaacttg
      661 tcaaggaaat aaggcgcgcg gctatatctc ggacagcacg gccgaggcta gagcggcggc
      721 cttttccgca tcagacatca cggtatcgag cacgaccggc ttgatcccca tctcgcttat
      781 cctctccgcc tcgcgtgcgt cttccgtatc tataaccatg acgtcgagaa agtccctgta
      841 gagtctcgca acgccggccg aggacacctc gagcccgagt cccctcatga gcttatcggc
      901 aggccccttg agcggcaccc ctcctataag cgggcttatg gcgataacac gtccgccaga
      961 agccctgagc gcttctccga tgcccttgac gttgagtatg gggccaatgc ttattattgg
     1021 gttactgggg caaagtatta tcatcctcgc gtcccttatc gcctcaagga cgcccggagc
     1081 gggcttcgcc ctttcgatat tcctgaagac cacctttaac acctcgggct tcatctttcg
     1141 cttgacgaaa tattcctgga agtgcatctc cccctcgtcg gttattatcc acgtctctac
     1201 gtcctcgtcc gtcatgggaa ggactttgat actgtcctcg ataccgaaag ccctagccag
     1261 gcgttcggtg acctgcacgg gcgtaaaccc ctcattcctg agacgggtcc tgtatatgtg
     1321 agtcgcgaag tcgatatccc cgatgttaaa ccaggtctcg gctccaaacc tcgaaagcgc
     1381 ggccaggcag gtgaacgtat cattcctgac gccccaccct ttgccggtgt ctataaatcc
     1441 cgaaagcctg tatatgatgg tatcgacgtc cggggaaatc cggagcccgt gtaaaactat
     1501 gtcgtcgccg gtatttacga ctaccgacag cggctcaccg cttatcacat gggatagacc
     1561 ttcgagaaat ttagcggcac ctacacctcc gccgagggca gttatcatca gggcagacct
     1621 tcgagagaag acaagattaa aaaagcttcc gtgtaacgac cactctgttc ttaagaaggg
     1681 cttaagaata tttgagcgtt cccgggctgt caagtgagca aagcgccggt ccggccgtcg
     1741 ccggaaaggt gaaaatcagg taaacgtgaa ccgatggttt gaaggggatg cttatgagtg
     1801 tccagccccg ggattgttga aactgaagag gaagtgatat aaatttctat aagaattcaa
     1861 gcacagaggg gaatataaat aagatgagtc tcaattttat caggaacaga cacagctggt
     1921 ttatcagggg catactaatc gtaatcgccc tggctttcat tataggtatc ggctacaacc
     1981 tcagcaattt cggcgctatt acgaatgtcc ccgacaggac cgccgccaag gtaaacgggg
     2041 aagacgtaag cctggtcaat ttttacatca tgagggacag ccttaaaaga cagatcgggc
     2101 aggaaggaga agtgcccgaa gaatatatga atcagatcaa tgtgatagca ctcaatcagc
     2161 tcatcaatct caagctcctg gctcaaaagg ccaagagcct cgggttcagg gtaacggacg
     2221 aggagctggg gagcgcgatt acgaccgacc cgaatttcca gatcgacggc aaattcgtgg
     2281 gcagggacag atacaggcag ttcgtcgagc aggctcttca tcaggaagtc ggggatttcg
     2341 agaatacata cagggagaga ctcctggccg agaagctcgc gaggttcctc gacgaaacgg
     2401 caattattac taacgaaaac ctgctcgagg tgtacaacaa gcagaatgaa aaggtcaacc
     2461 tctactacat atccttcccg gcggatgatt ttgccggctc ctataccccc ggagagaagg
     2521 agataaagga ttactaccag aaacacaaag gcgaattaaa gaccgccgag acgaggcaga
     2581 tacgctattt cacactgagc ccggaaacgc tcgaaaagag tatagtggtt tcggaggacg
     2641 aaatcggttc ttactacacc gcataccccg aagaattcaa atcacaggac gggaagcaga
     2701 tccccctttc agacgtgaag ggcgatatag aatccaagct caaagcccag aaagccgaag
     2761 cgctccgcca gcagctgatg gccagaatag ataacccggg tgaaggcggc cccgccggca
     2821 tcgatacgct cgccaaggaa tattcggtag agaccatcaa tgaaagcaag cctttcacga
     2881 tgacggacat tatggccgac gtgccgccta tggtcacgcg tcaggcgttc agcggcgata
     2941 agggcacggt atctctgatc ccggttggca cgagcatttg ggtcatggag gtaacgagca
     3001 taacacctcc gaaggagcaa accttcgagg aagcggaatc aaatataacc gcaacactca
     3061 aacacgagaa gtccctggag ctggcaaagt cgaaagcgga agaaacgctt aagaagctga
     3121 ggggcgtcaa gaacgagaac ctgcccgccg agacaaaaaa gctcggactc cagttaaagg
     3181 agacggggtt attctcaagg tcggagaaag tgccgcagct gaattccccg ataataggct
     3241 cggacgcatt cgagctggac gtcaaagccg gcgtaccgaa ccgtatctac gacgacaagg
     3301 acaagttcgt aataatctca gtcaaggagc tgatgagcgc aagccccggg gatttcgacg
     3361 aggtaagggg cgccttgatg cagcaggaac tcgacaagca gcgctcgcag gtcatacaga
     3421 aggtgattca ggacatgagg cggcaggcgg aaatcgtacc gaacaacaaa ctattcccgg
     3481 cgcaggggtg acggaagcgt caaggggtcg gaaacggatt gtctagaagg gctctgataa
     3541 taggacggga tagggactat tgctatgtcc tcagggagtt cctggctctg agggagctgt
     3601 atgtaacagt agtgctcgat tacaaggaag gcctggacag actcttttat gaaaaacctg
     3661 acctgacgat cttcgagagc tcgggccctg agcccgtcag ggcatgtgtc gattcgataa
     3721 gagaatcgaa cgagttcgaa atagttgatt tcggcagcgg ccaggccata aaaaccgggg
     3781 cgaagcccgt actgctcttc gacgataaat cgcgtatcaa ctctcttttc gatttcctga
     3841 agtcaggttt ttctcagccc cagcccgagc agccgcccga caccggagac gagggcagtc
     3901 tcgaaagcac gttctacccg agcctcctcg tagatataaa tttaaagaaa aaaagcgggg
     3961 tactttccat aacgtccagc accaacctga tcatttattt tatcaacggc aaccctgtat
     4021 tcgccgaggg aggagacatc gaaacggcca tcggcaggat actcctcaac agcggcaaaa
     4081 tcgacgaaga gacctacgaa aaagcagtgg acatagcctc aaagaacaaa cagaaattcg
     4141 gacagatact ctttgaaatg ggcatcactt caccccacga gctcaacagc ttccttgaat
     4201 tccagatgca ggaaaaaatt ctcaagggct tctattacac caaggggaaa tacgaattca
     4261 agagcgggga cgaattcgcg gacaggatcg ttttgtacca ggtcgacgta tccaaggtta
     4321 tctacgaagg agtgaagagg tatatagacg taaacgacct cgaagaagca aacccggcga
     4381 tcattatcga tccgagatta aggaccgata tcaacagcct cgggctcaag cccaaagagc
     4441 tcaggttcgt acagctttta aaggatagag ccaccgtcgt ggaagtgctc gaagggtcga
     4501 gactcgaaag acccgaagcc ctcaagcttc tctactttct ttcgttattc cggctggtaa
     4561 aaatgagcgg catatccacg gacgatatag gcagggcatc aatcgagaaa aggatcaggg
     4621 agagtgaaac agggccggga gtgagcggcg ccccggagtt tcattcggac atggagagtg
     4681 tgttcgccga attaaaagag ctcgaatccg aagaaagccc ggaggtcggg ctcggtacag
     4741 ggcacagcga taccgtcgat gaaatcgtgt atcagggcgc ggagcaggag ccggaagcca
     4801 aaagcgcggc cgggaactcc cacgcgttcg agtctcgatt cggcttcagc gaaggagcta
     4861 gtgaaccgga agtcccattt gaaggcagtc cgaatggcgg attcgagagt cccggaccgt
     4921 ccgcgtatga agagaataat aaagaagagg aatattatat attagatacc gagagggaag
     4981 aacctcaagc cggagagccc cagggaagcc cccctgtata tgaaatagag ctcgacctcg
     5041 acctcgaccc ggaagccggg gaaaacggtc aatcggcctg gggcaaagct ccgactacac
     5101 cgcaatttca ggaaccggcc cggccaggag gtgaggaccg cgaaggc
//
LOCUS       DASSJH010000147         5914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_790600, whole genome shotgun sequence.
ACCESSION   DASSJH010000147 DASSJH010000000
VERSION     DASSJH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790600"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFJ67_03200"
     CDS             complement(<1..325)
                     /locus_tag="VFJ67_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289421.1"
                     /translation="MSETTKKAASKVKELTEPVKEFSGLVKENYLNGLDFTFSLLEQN
                     MKVLGAQADHMFDLEKEFVTNLSGLYKDFPKDLPFVKDLPFDGGVKKVSEQLERYIAY
                     RKEQMQ"
     gene            657..1235
                     /locus_tag="VFJ67_03205"
     CDS             657..1235
                     /locus_tag="VFJ67_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7289422.1"
                     /translation="MKYIRIELLPDLLCYARLLLVPVVLALALLALPFWTGIALLAGG
                     LTDVLDGAIARRRNIVTAYGSRLDSIADTLMEISAAAALLLLRPDIFTTHPFILGTWI
                     AIEASWIILGWIKFRRIADLHLYLTKAGGVLAYAFIVYTFVVGYSEAFFRATAAVLIV
                     SSLECLLLVLFARSVDENMKSLYHAYRAGRLF"
     gene            complement(1330..1701)
                     /locus_tag="VFJ67_03210"
     CDS             complement(1330..1701)
                     /locus_tag="VFJ67_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289423.1"
                     /translation="MRTLIYTILMLTAAASFSSAQEVGSDARLLARAETHTTSVTYNI
                     RNGKTFKSDTPRLSPAGSIDDTAGISRAVSEELDSGKSSGLSYNQPTLKAEDLPLVMS
                     SPDRKTISVVLPPFLYFKTQF"
     gene            complement(1890..2675)
                     /locus_tag="VFJ67_03215"
     CDS             complement(1890..2675)
                     /locus_tag="VFJ67_03215"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7289424.1"
                     /translation="MNVKELFDLKGKTAVVTGGYTGIGRQMALALAEAGADVVVCARN
                     LEGCETTAREIAVSAGVKTLALKCDVSAEADVVNMVSGTVAEFGGLDILVNNAGIAMG
                     GLPEDTKLEDWDRILGVNLTGTFLCAREAAKVMIKAGRGKIINISSVMGYQTTSVVSA
                     PAYVASKGAVVTLTKDLAVRWIKHNINVNAIAPGWFPTNMTEPVLSPEFMNMGKELLA
                     SIPAGRFGGEDDLKGVTVLLASRASDYITGEVIVVDGGTLSRY"
     gene            complement(2789..4195)
                     /locus_tag="VFJ67_03220"
     CDS             complement(2789..4195)
                     /locus_tag="VFJ67_03220"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET7289425.1"
                     /translation="MASKNTRTEYDSMGQMEVPSNAYYGAQTARAIENFPISGIRKHS
                     LLIKAMGMIKLAGAESNMQLGLLSKKTGGAIVRAAKEVIDGKLDDHFVLDVFQTGSGT
                     STNMNTNEVIANRANEITGAKKGDKSGIHPNDHVNMGQSSNDVYPTAIHISAILAIND
                     VLLPALKNLERALTAKAREFDKIVKIGRTHLQDATPIRLGQEFSGYASMIKHGIKRVE
                     HAREELSELAIGGTAVGTGINTHPKFAGLVVKRLSKMTGTKLTEAENHFEAQGSKDAV
                     VQASGSLKTLAVSLMKIANDIRWLGSGPRCGIGEIMIPPVQPGSSIMPGKVNPVIPEA
                     VCQVAAQVIGNDLTITIGGTFGNFELNVMMPVKGHNLLQSIELLGKVSQVFTDKCIKG
                     IKADRKRCEEMIEKSLAMCTSLAPVIGYDNAAKIAKEAYATGKTVREVALEKKVLPAE
                     KLKKLLDPWSMTEPGTKE"
     gene            complement(4501..>5914)
                     /locus_tag="VFJ67_03225"
     CDS             complement(4501..>5914)
                     /locus_tag="VFJ67_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HET7289426.1"
                     /translation="VKAGGLPAVFAMTIFAGAVEVLMALFLRRLKPIFPPAVSGFIVL
                     VIGIELGLVALGNMLDVKGFGKPGYTENLLVGIIAISIIMGFSVWFKGIMRLLCSLFG
                     IAIGFIAALLLGILPASSIELFKSSALFALPDPGYISYSFNASLIPAFLTAGIAASLR
                     TIGVVTTAEKINDSDWRRPDFGPIKKGMLADGIGCMLGGLMGAPGMNATPGIVGLSQA
                     AGSTSRYMAFPTGIFLIVLAFFPKISALFLMLPLSVVGASLVVSGAFMISGGIEIMTT
                     RNLDSRMTYVVGVSLLLGIGRKVFHGYFNSFPEWLRLMTDTTLSIALISSVALVLIFR
                     LGIKKKDIFLYDKALPSCEHLREHLKSREKEWRLDDEIIDHAVASTEQIIHHITEAKL
                     TQGPIKLEAAYDEVYLEIIIEYKGTLLTLPAVGAKHRHYVEEEAFVHGIKDFLTGVYP
                     DRIETSSKHDEAKIRLFFNV"
BASE COUNT         1126 a         1666 c         1644 g         1478 t
ORIGIN      
        1 tctgcatctg ctccttgcgg tacgcgatgt agcgctcgag ctgctcggat accttcttga
       61 cgccgccgtc aaagggaagg tcctttacga acggcaggtc tttcgggaaa tccttgtaga
      121 gcccgctgag gttcgtaacg aattccttct cgaggtcgaa catgtgatcg gcctgtgcgc
      181 cgagcacttt catgttctgt tcgaggagag agaacgtgaa atcgagacca ttaagatagt
      241 tctcctttac gagaccggag aattccttta ccggctcggt caattcttta acctttgaag
      301 ctgctttctt tgtagtttcg gacatggcga aacctcctga tattttttct aaagaacaat
      361 ataaacagct tgagagggat gtcaagggtt tttattcgca attgcacaaa ttaataatgc
      421 tatgattatg ataggttatg tatgttaagc ctgcgtgaag agacgcatat gctaataaag
      481 cccggctggt ctgccgtata taccgtttaa atgccgtctg tgcgatgagt gtccgctcat
      541 ccgtgcgctt catgcggttt acccgagaaa gggggaatga cggtcggcgg agcggataat
      601 tttttagttg gaacgttctc tctgttaaga taagactgcg cccgggataa ctacgaatga
      661 aatatatacg gatagagttg ctccccgacc tcctctgcta cgcaaggctc ctcctcgttc
      721 cggtggtgct cgcgctcgcc ctgctcgccc tgccgttctg gacggggata gctctcctgg
      781 caggaggtct caccgacgtg ctcgacggcg cgatcgcgcg gaggcggaac atcgtaaccg
      841 cttacggcag caggctcgat tccatagccg acacgctcat ggagatctcg gccgcggcgg
      901 cgctccttct gctcaggccc gacatcttca ccactcaccc gttcatactc ggcacgtgga
      961 tcgcgatcga ggcgtcgtgg attatcctcg ggtggataaa gttcaggagg attgcggatc
     1021 tccatctcta cctgacgaag gcagggggcg tcctcgctta cgcgttcatc gtctatacgt
     1081 tcgtagtcgg atacagcgag gcgttcttcc gcgcgacggc tgcggtacta atcgtttcct
     1141 cgctcgaatg tctgctgctc gtgttgttcg cccggagcgt ggacgagaac atgaagtcgc
     1201 tctatcacgc gtaccgcgcg gggaggctat tttgaatgtc ggagctcgct cgctgttaca
     1261 agtattagca ggagcggctt tcccgaaaca agttcaggac gggccagccg cgattattta
     1321 actccgatat cagaactgtg tcttgaagta gaggaacggc ggaagcacga cgcttatggt
     1381 ttttctgtcc gggctcgaca tgactagagg aaggtcttcg gccttgagcg taggctggtt
     1441 atatgacaga ccggaactct tcccgctatc gagctcttca ctgacagccc gcgagatacc
     1501 ggccgtatcg tctatggagc cggcgggtga aagcctcggg gtatcgcttt taaaggtctt
     1561 tccgttccgg atgttatatg taacggaagt ggtgtgcgtt tccgctctcg cgaggagcct
     1621 cgcatcagag ccgacctctt gagcggacga gaatgacgcc gccgcggtta acatcaatat
     1681 cgtatagatt agcgttctca atctttttct ccttatgtaa ctcttagaca caattattag
     1741 acgaatggat gcggacgatt tggacaggtt gaagaaataa attttccgct tcaactgttc
     1801 tggcgggtat tttcttgttt ggaaggcggt tacgtctgcg actttttcgg tacggaccct
     1861 tttcggaggc caggatttac atcccggact tagtacctgg acagcgtgcc gccgtccacc
     1921 actataactt cgcccgttat gtagtctgac gcgcgcgacg cgaggagcac ggtcacgccc
     1981 ttgaggtcgt cctctccccc gaaccttccg gccgggatcg aagccaggag ctccttcccc
     2041 atgttcatga actcggggct cagcacgggc tctgtcatgt tggtcgggaa ccatccgggg
     2101 gcgatcgcgt ttacgtttat gttgtgcttt atccatctca cggcgaggtc cttcgtgagt
     2161 gtcacgaccg cgcccttgga ggctacgtac gcaggggcgc tcacgacgct cgtcgtctgg
     2221 tagcccatga cggacgagat gtttattatc ttccctctcc ccgcctttat cataactttc
     2281 gccgcttccc tcgcgcagag gaacgtgccc gtgaggttca cgccgagtat cctgtcccag
     2341 tcttcgagct tcgtatcttc ggggaggccg cccatggcga tgccagcgtt gttgacgagt
     2401 atgtcgagcc caccgaattc agcgaccgtg cctgagacca tattcacgac gtccgcctcg
     2461 gcggagacgt cgcacttgag agcgagcgtt ttcacgccgg cgctcacggc tatctcgcgc
     2521 gccgtggtct cgcacccctc gaggttcctc gcgcacacga cgacgtcggc cccggcttcg
     2581 gcgagagcga gcgccatctg cctgcctatg cccgtgtacc ccccggtgac cacggccgtt
     2641 ttccccttga gatcgaatag ctcttttacg ttcatgattg tctccgatgt ccgatgttat
     2701 tagtaaggtt agcataagcc cgggaagacc cgtccggatt ttcccgggct cgaaagtttt
     2761 cagcaggata atttagttgg ggtaataact actccttcgt ccccggctcc gtcatcgacc
     2821 acgggtcgag gagcttcttc agcttctcgg ccggaagcac tttcttttct agtgcgacct
     2881 ctctcaccgt cttcccggtc gcgtatgctt ccttcgctat cttcgccgcg ttgtcgtatc
     2941 cgattacagg ggcgaggctc gtgcacatgg cgaggctctt ctctatcatc tcttcgcagc
     3001 gcttcctgtc ggccttgatc ccctttatgc acttgtcggt gaagacctgc gatacctttc
     3061 cgaggagctc tatcgactgg agcaggttgt gcccctttac gggcatcatg acgttcagct
     3121 cgaagttccc gaacgtcccg ccgatagtaa tggtgaggtc gttccctatc acctgcgccg
     3181 cgacctggca gacggcttcg gggatgacgg ggttcacctt gcccggcatg atggacgaac
     3241 ccggctgcac gggcggtatc attatctccc caatgccgca gcgggggccg ctaccgagcc
     3301 acctgatgtc gttcgctatc ttcatcaggc tcacggcgag ggtcttgagt gagccgctcg
     3361 cctggactac cgcgtccttc gagccctgag cctcgaaatg gttctccgct tccgtgagct
     3421 tcgtccccgt catcttgctg agcctcttaa cgacgagccc ggcgaatttg gggtgcgtgt
     3481 ttatgcccgt gcccactgcc gtcccgccga tcgcgagctc ggataattct tccctcgcgt
     3541 gctccactct ctttatcccg tgctttatca tgctcgcgta tccgctgaac tcctgcccga
     3601 gcctgatagg cgtcgcgtcc tggaggtgcg tgcgtccgat ctttactatc ttatcgaatt
     3661 cgcgcgcctt cgccgtgagc gccctctcga ggttctttag cgccggcaga agcacgtcgt
     3721 tgatcgcgag tatagcagag atgtgaatgg cggtcggata tacgtcgtta ctcgactggc
     3781 ccatgtttac atggtcgttc gggtggatgc cggacttgtc ccctttcttg gctcctgtta
     3841 tttcgttcgc gcggttggct atgacctcgt tcgtgttcat gttcgtcgag gtgcccgacc
     3901 ccgtctggaa gacgtcgagt acgaaatggt cgtcgagctt cccgtcgatg acctccttcg
     3961 ccgccctgac tatcgcgcct cccgtttttt tgctcaagag accgagctgc atgttggact
     4021 ccgcccccgc gagcttgatc atccccatcg cctttatcag aaggctgtgc ttgcgtatac
     4081 cgctaatcgg gaaattctct atcgccctcg cggtctgggc gccgtaatat gcgttcgacg
     4141 ggacttccat ctgccccatc gaatcgtatt ctgttctggt atttttactt gccatatacc
     4201 cctccttggg ttttgtgttc agggagttta attatgccat aacggggata tttttctaat
     4261 ggaaagaagg atagtgacga ctcgcgccgg ggttgtgaag ggacttgtcg cgtattgaaa
     4321 gagccgccac acccgttcaa cccttcgaca agctcagggc gaacgggtgg aggttgccct
     4381 gtgtgcgggt gcccaagccg ttcgtactga gctcgtcgaa gtaagaaggg cttgggctaa
     4441 tctgttggtt tagggttgca ggcatattag aaacataaaa catactaagt gtgtgctgcg
     4501 ctacacatta aaaaaaagcc ttatcttcgc ttcgtcgtgc ttcgatgaag tctctatcct
     4561 gtcgggatag acgcccgtga ggaagtcctt tatgccgtgg acgaacgctt cttcctcgac
     4621 gtaatgcctg tgcttcgctc caaccgccgg gagggtgagg agcgtaccct tgtattcgat
     4681 gattatctcc agatacacct cgtcgtacgc ggcctcgagc ttgataggtc cctgtgtgag
     4741 tttcgcctcg gttatatggt ggattatctg ctctgtggag gcgacggcgt ggtcgattat
     4801 ctcgtcgtcg agcctccatt ccttctcacg ggattttagg tgctcgcgga ggtgctcgca
     4861 tgaggggagc gccttgtcgt agagaaatat gtccttcttc tttatgccga ggcggaatat
     4921 caggacgagc gcgaccgagg aaatgagggc gatcgagagg gtcgtgtccg tcattaacct
     4981 cagccattcg gggaagctgt tgaaataacc gtggaagacc tttctcccga tccccagcag
     5041 gagcgatact cctacgacgt acgtcatccg cgagtcgagg ttcctcgtcg tcattatctc
     5101 gatccctccc gagatcatga acgccccgct cacgacgagg gacgcgccta ccacggagag
     5161 ggggagcatt aaaaagagcg cggatatctt ggggaagaat gcgagcacta tgaggaatat
     5221 cccagtcggg aaggccatgt agcggctcgt cgagcccgcc gcctgtgaga ggccgactat
     5281 acccggcgtc gcgttcatgc ccggtgcgcc catgagacct cccagcatgc accctatccc
     5341 gtcggcgagc atgccttttt ttatgggtcc gaagtcggga cgcctccagt ctgaatcgtt
     5401 tatcttctct gctgtagtga cgacgcctat cgtccgtaac gatgcggcta tacccgcagt
     5461 gaggaaggcc gggatgagag acgcgttgaa gctgtacgat atgtagcccg ggtcgggcag
     5521 cgcgaacagg gccgagcttt tgaaaagctc gatcgagctt gcgggcagta tgccgagcag
     5581 cagggcggca atgaaaccga ttgctattcc gaagagcgag cacaatagcc tcattatccc
     5641 cttgaaccag acgctgaacc ccatgatgat cgagatagcg attatgccca cgagcaggtt
     5701 ctccgtatag ccgggctttc caaatccctt gacgtcgagc atgttgccta gcgccacgag
     5761 cccgagctct atgcctatga cgaggactat gaaccccgac accgcagggg ggaatatggg
     5821 tttcaggcgg cggaggaata aagccatcag cacctctaca gcgcccgcga atatagtcat
     5881 ggcgaatacg gccgggagcc cgcccgcctt gacc
//
LOCUS       DASSJH010000148         5121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_895228, whole genome shotgun sequence.
ACCESSION   DASSJH010000148 DASSJH010000000
VERSION     DASSJH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895228"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1132
                     /gene="pheS"
                     /locus_tag="VFJ67_03230"
     CDS             113..1132
                     /gene="pheS"
                     /locus_tag="VFJ67_03230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7289427.1"
                     /translation="MIEMLEDVKRQAEAELSSVNDEITLQNLKAKFVGRKGVITEILK
                     GMKDIPDSDRPRMGKLINETKTFVEGLIDNRLDAIREEKKRRSLHEEKIDVTLPGRGV
                     PIGARHPISQVMEEITAIFERMGFEVAEGPEVETDYYNFEALNIPKNHPARDMQDTFY
                     ISDEIVLRTHTSPVQIRVMEKRKPPVKIIAPGKVYRCDSDISHTPMFHQIEGLLVDEG
                     VTFGDLKGVLTEFVRLVFGEGVGVRFRPSFFPFTEPSAEVDIACLICGGKGCRVCKNS
                     GWLEILGCGMVDPEVFKSVGYDPDKYSGFAFGMGIERITMLKYGINDIRLFFENDLRF
                     LKQFT"
     gene            1306..1860
                     /gene="dcd"
                     /locus_tag="VFJ67_03235"
     CDS             1306..1860
                     /gene="dcd"
                     /locus_tag="VFJ67_03235"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006343078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET7289428.1"
                     /translation="MGVKSDTWIRKMAKEHNMIDPFVDGQVREGAISYGLSAYGYDIR
                     VSDEFKVFTNVYNTVVDPKHFDDKSFVEITSEVCIIPPNSFALARTVEYFKIPRSTIT
                     LCVGKSTYARCGIIVNVTPFEPEWEGFVTLEISNTTPLPARIYANEGIAQVLFFEADD
                     MCEVSYADKKGKYQKQVGITPPKV"
     gene            2049..4517
                     /gene="gyrB"
                     /locus_tag="VFJ67_03240"
     CDS             2049..4517
                     /gene="gyrB"
                     /locus_tag="VFJ67_03240"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET7289429.1"
                     /translation="MEVKSDLNKESQTKVDSSYTAEKIKVLPGLEAVRKRPDMYIGDT
                     STRGFHHLVFEVLDNSVDEALAGYCDRIGVTIHVDESVTIEDNGRGIPVDKHKETGKS
                     AAEVVMTMLHAGGKFEGKVYQVSGGLHGVGVTVVNALSEYLNLEIRREGKVWFQKYER
                     GNAVSDLKETGKTDRTGTKIRFKPDPQIFEIYEFSYDTLAHRIRELAFLNRGLVIQLT
                     DERTGKSQEFYYKGGIVAFVEELNKNKKPLHPDPIYVSGEKDDIIVEVALQYNDGYTE
                     TIFCYANNINNIEGGTHLTGFRGALTRTVNKYAEDKGLLKNAKVTLSGDDTREGLTGV
                     ISVKLPDPKFEGQTKTKLGNTDVKGIVETLLNDKLGTFFEENPSVARKIIEKTVDAAR
                     AREAARKARELTRRKSALESGSLPGKLADCQERDPELCELFIVEGESAGGSAKQARAR
                     YNQAVLPLRGKILNVEKARFDKMLGNEEIRTLITVLGTGIGSDDFDIAKLRYHKIILM
                     TDADVDGSHIRTLLLTFFFRQFSEVIDKGHLYVAQPPLYRVKRGKEEKYLKDDHEYED
                     YFLHHGTEGIVLNVTQNGNGTKEIKGSKLFDIIKKSISYGKALERIGKWGRDMRIVDA
                     FASREGIKKGHLKTDKSEELDVHLQAIKEWIEARHPEITGLDWQLLDDSEHDASKVSY
                     SFRESGRQRSTLIDFDFLNSPEFNEIRNLRESIKAVGDPPYGIHDDGAAVELPSLSAV
                     TEHILTRGKKGMTIQRYKGLGEMNPEQLWETTMNPETRVLKKVMVEDAVDTDEIFTIL
                     MGDEVEPRKEFIETNALNVVNLDV"
     gene            4550..4912
                     /locus_tag="VFJ67_03245"
     CDS             4550..4912
                     /locus_tag="VFJ67_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7289430.1"
                     /translation="MAESVKNINQIPTEKVSAGSSTYSQVLIGPDEGPNFAMRRFIME
                     PGGGIPAHTNTVEHEQYVLRGRAKVGIGDNVYEVKAEDVLYIPAGVPHWYEVIGSENF
                     EFLCMVPNRDDRITLLQD"
BASE COUNT         1392 a         1283 c         1424 g         1022 t
ORIGIN      
        1 cggagagtcg cactcaagcc catattccgt gattcggccg gcggagtaga tgtaaattct
       61 cgttatggta gattattgtc cggatttcga tggttttccc ggagtcatat agatgattga
      121 gatgctcgaa gacgtaaaga ggcaggctga ggccgagcta agctcggtga acgacgagat
      181 cacgctccag aacctcaagg ccaagttcgt cggcaggaag ggcgttatta cggagatact
      241 gaagggtatg aaggacatcc ccgattccga ccgcccgcgc atggggaagc tcatcaacga
      301 gacgaagacg ttcgtcgagg gcctcatcga caacaggctc gatgccataa gggaggagaa
      361 gaagaggcgc tcgctccacg aggagaagat agacgttacg cttccgggaa ggggcgtgcc
      421 cataggtgcg aggcacccca tatctcaggt catggaagag ataacggcca tattcgagag
      481 gatggggttc gaggtcgctg aaggccctga agtcgagacc gattactaca acttcgaggc
      541 gctcaacatc ccaaagaacc accccgcgcg cgacatgcag gatacgttct acatatccga
      601 cgaaatagtg ctgaggacgc atacatcccc ggttcagata cgcgtcatgg agaagcgtaa
      661 gccgccggtg aagattatcg ctcccgggaa ggtctacagg tgcgacagcg atatatctca
      721 cacgcctatg ttccaccaga tagaggggct cctcgtcgac gaaggcgtca cgttcgggga
      781 cttgaagggg gtgctgaccg agttcgtcag gctcgttttc ggggaaggcg tcggggtgag
      841 gttcaggccg agcttcttcc cgttcactga gccgagcgcc gaggtggaca tagcgtgcct
      901 catatgcgga ggaaaggggt gcagggtatg caagaattca ggctggctcg agatactcgg
      961 ctgcggcatg gtcgatcccg aggtgttcaa gagcgtcggc tacgaccctg acaagtattc
     1021 gggcttcgcc ttcggcatgg gtatcgaaag gattacaatg cttaaatacg gcataaacga
     1081 catcaggctc ttcttcgaga acgacctccg tttcttaaaa cagttcacct gatcggcctc
     1141 tgatttttct ttcatgtagg gtggattttt ctttgtcttt atttaatcct tgtctcgatt
     1201 ccgccagctg acgacccccc tctccccccg attgagttaa atccccgcct gtggtaatat
     1261 gcttttcctc taagcatcat aatcctcata atcaggagca aggaaatggg agttaaatct
     1321 gacacctgga taaggaaaat ggcaaaggaa cataacatga tagacccctt cgtcgacggg
     1381 caggtgaggg aaggggcgat ctcttacggt ctatcggcat acggatacga cataagggtt
     1441 agcgacgagt ttaaggtctt caccaacgtc tacaacaccg tggtcgaccc caagcatttc
     1501 gacgataaat cgttcgtcga gattacatcg gaggtctgca taatcccgcc aaattccttc
     1561 gctctcgcaa ggacggtcga gtactttaaa attccgagga gcacgataac cctctgtgtc
     1621 gggaagtcca cgtacgccag gtgcggaatc atagtgaacg tcaccccgtt tgagcccgag
     1681 tgggaaggtt tcgtcacact ggagatatcg aacactaccc ctttgccggc gagaatctac
     1741 gctaacgaag gcattgcgca ggtgcttttt ttcgaggccg acgacatgtg cgaggtctcc
     1801 tacgccgaca aaaagggcaa ataccagaag caggtcggga taacacctcc caaagtctga
     1861 gcgataacgg ccggaatgcc ccgtcctgac ttgaaaaatt tggataaatt cccggcttcc
     1921 gtccggttca ggaattactt cgaaaaatat actaaaaccc cttgatttta cttccatttt
     1981 tcgctcctca tggtataata ttaactcatt aatttccccc ctgcagtcat gttcggaggt
     2041 agtcgatttt ggaagtaaaa tcagacctga ataaagagtc tcagaccaag gtagacagct
     2101 cgtacacagc agaaaaaatt aaagtccttc ccggcctcga agccgtcagg aagcgtcccg
     2161 atatgtacat cggggacacg agcacgcggg gtttccacca tctcgtattc gaggtcctgg
     2221 acaacagcgt ggacgaggcc cttgccggtt attgcgacag gatcggcgtt accatccatg
     2281 tggacgaaag cgtcacgata gaggacaacg gaaggggcat acctgtagat aaacacaagg
     2341 aaacgggtaa gtccgctgcc gaagtcgtca tgacgatgct ccacgcgggc ggcaagttcg
     2401 aagggaaggt ctatcaggtt tcgggaggac tccacggagt cggcgtcact gtcgtaaacg
     2461 cgctttccga gtatctcaac ctcgagatca ggagagaggg gaaggtctgg ttccagaaat
     2521 acgagcgcgg caacgccgtt tccgatctca aggagacggg caaaaccgac aggacgggca
     2581 cgaagataag gttcaagcct gacccccaga tattcgagat atacgaattc agctacgata
     2641 cgctcgccca caggataagg gagctcgcgt ttctcaacag gggcctcgtc atccagctta
     2701 ccgacgagag gacgggaaag agccaggaat tctattacaa gggcggaata gtcgccttcg
     2761 tcgaggagct caataagaac aagaagccgc tccatcccga cccgatatac gtatcgggag
     2821 aaaaggacga catcatcgtg gaagtggctc tccagtataa cgacggttac acggagacga
     2881 ttttctgcta cgccaataac ataaacaaca tcgagggcgg cacccacctc accgggttca
     2941 gaggcgcgct tacgaggacg gttaacaaat acgcggaaga taaaggcctt ctgaaaaatg
     3001 ccaaagtcac tctcagcggc gacgacacga gggaagggct cacgggcgta ataagcgtaa
     3061 agctgcccga ccccaagttc gagggacaga ccaagaccaa gctcggcaat accgacgtaa
     3121 agggcatcgt ggagacgctc ctcaacgaca agctcgggac attttttgag gaaaaccctt
     3181 cggtcgcgag aaagataatc gaaaaaaccg tcgatgcagc acgggcgagg gaggctgcga
     3241 ggaaggcgag ggagctcacg aggagaaaga gcgctcttga atcgggatct ttaccgggaa
     3301 aactcgcaga ctgtcaggag cgcgaccccg agctgtgcga gctgttcata gtcgagggtg
     3361 aatccgccgg cggctccgca aaacaggcga gggcaaggta caatcaggcg gtcctgccgc
     3421 tcaggggaaa gattctcaac gtcgagaagg cgagattcga caagatgctc ggaaacgagg
     3481 agataaggac gcttatcacg gtcctcggta cgggcatagg ctcggacgac ttcgacatcg
     3541 cgaagctcag ataccacaag ataatactta tgaccgacgc cgacgtcgac ggctcacaca
     3601 taaggacgct gctgctcaca ttcttcttca ggcagttctc tgaagtgatc gacaaaggtc
     3661 atctgtacgt cgcccagccg cccctctaca gggtgaagcg cgggaaggaa gagaaatatc
     3721 tcaaggacga ccacgagtac gaggactatt tcctccatca cggtaccgaa gggatcgtat
     3781 tgaacgtcac ccagaacggc aacgggacga aggagataaa agggtctaag ctcttcgata
     3841 tcataaagaa atcaatctcg tacgggaaag cgctcgaaag gatagggaaa tggggaaggg
     3901 acatgaggat agtggacgcc ttcgcgagca gggaaggcat caaaaaagga catctcaaga
     3961 cggataaatc ggaagagctc gacgtccatc tgcaggcgat caaggagtgg atcgaggcaa
     4021 gacaccctga gattaccggt ctcgactggc agcttctcga cgactccgag cacgatgcct
     4081 ccaaggtttc ctacagcttc agggagagcg gcaggcagag gagcacgctg atcgatttcg
     4141 atttcctcaa ctcgcccgag tttaacgaga taaggaacct ccgtgaatcg atcaaagccg
     4201 tcggagaccc gccctacggc attcacgacg acggggccgc ggtcgagctg ccgagtcttt
     4261 ccgccgtgac ggagcacatt ctcacgcgcg gcaagaaggg catgacgata cagcggtaca
     4321 agggtctcgg cgaaatgaac ccggagcagc tctgggaaac gacgatgaac ccggagacgc
     4381 gtgtcctcaa gaaggtaatg gtcgaggacg ctgtggacac ggacgagatt ttcacgattc
     4441 tcatggggga cgaggtcgag ccgaggaagg aattcatcga gactaacgcg ctcaacgtcg
     4501 tcaacctcga cgtataatct cctgaaccac tgacccggaa cgaagcaata tggccgaatc
     4561 cgtaaaaaat ataaatcaaa tccccacaga aaaggtaagc gccgggagct ccacgtacag
     4621 tcaggtgctc atcggccctg acgaaggccc caacttcgct atgaggcgct tcataatgga
     4681 gccgggcgga ggaattcccg cacacacgaa cacagtcgag cacgagcaat acgtgctccg
     4741 cggccgtgcg aaggtcggta taggcgataa tgtttacgag gtgaaagccg aggacgtcct
     4801 ctacatccca gcgggagtcc cccactggta cgaagtcatt ggcagcgaga atttcgagtt
     4861 cctctgcatg gtcccgaatc gggacgatag aattacactg cttcaagatt aactattcga
     4921 taacccttca aaagatatag taaaattcaa atgcggaaaa tataacgtaa gaattcggca
     4981 atatgtagcc gtgagattga aattattgaa ttattgtatt actctatcct tcttgtcatt
     5041 tttttgacgc aagtaaagtt tatttggggg agcggtatct ttaggcatga aattaattaa
     5101 attcgttgga gtaaatctac a
//
LOCUS       DASSJH010000149         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_682759, whole genome shotgun sequence.
ACCESSION   DASSJH010000149 DASSJH010000000
VERSION     DASSJH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682759"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..231
                     /locus_tag="VFJ67_03250"
     CDS             34..231
                     /locus_tag="VFJ67_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019385778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HET7289431.1"
                     /translation="MPAKIRCPRCGKKSLWEGNRWRPFCSERCKMSDLGAWVTGGYTI
                     PGDPEEDTGHDDARNDDSEDR"
     gene            273..845
                     /gene="efp"
                     /locus_tag="VFJ67_03255"
     CDS             273..845
                     /gene="efp"
                     /locus_tag="VFJ67_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7289432.1"
                     /translation="MGVVQTNQFHKGLKIEYEGGIWEIIEYSHSKMAQRSAVMKTKIR
                     NVVTGAVQEKNFRSGDSFRVPDTERKSMQFLYKDEIGYHFMDLDTYEQHTLSAAQVGE
                     VASFIKDQQEVTVLYFNGEPMGVELPTAVELEVRDTEPGMRGDTVTGATKPAVLETGA
                     TIQVPLFIDVGDVIKVDTRSGEYLERMKKK"
     gene            1056..1628
                     /locus_tag="VFJ67_03260"
     CDS             1056..1628
                     /locus_tag="VFJ67_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7289433.1"
                     /translation="MDIDIELMLKAKSGDDGAFSELMRRHYKAVVNYIYRYTNVKENS
                     EDLAQEVFLRIYRSRKNYRPDAKFSTWLYKIATNVSLTHVKTRKVNQSLDEMEDERGG
                     IGDPDIDVADDLIYRKEIKEVIFQAMESLPEREKVAIMLCKYEGLSYEETAEVLECTV
                     GAVKAYVHRGRMKLIDKLKPYLPGGGEYGL"
     gene            1618..2124
                     /locus_tag="VFJ67_03265"
     CDS             1618..2124
                     /locus_tag="VFJ67_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7289434.1"
                     /translation="MGCKDVREMIAGYSSGDLPDSGRQAVESHVAECKECGLYLSRYE
                     GVWNLLDEWREIEPRTDFVSGFWAQVAKEERKPDRGILKKLLHGWGLAGAFATVLVVG
                     VFTFAMLRPQSGIVGYADNDTRDEQLLHELDSVTASDAADSDALAIYGPWDNGVEIMR
                     INGYGDQN"
     gene            2121..2540
                     /locus_tag="VFJ67_03270"
     CDS             2121..2540
                     /locus_tag="VFJ67_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289435.1"
                     /translation="MRTSLALTVMMCLFSVSAFGLVSGEIHYKWWKNPKIVQEMELTE
                     DQVDAIEDIFNSYREQIIDSQKELKKEEVHLLDILRKPECSRDEVMQITDNIEDLRAN
                     LTRIKVDMLLKIKNVLTSEQEETLHSIRDRYRGGRSR"
BASE COUNT          696 a          601 c          777 g          521 t
ORIGIN      
        1 cttgaaaact atttttgtta ggaaattatt tcaatgccgg ctaagataag atgccccagg
       61 tgcgggaaga aatctctctg ggaggggaac aggtggagac ccttctgctc ggagaggtgc
      121 aagatgtcgg acctaggcgc atgggtgacc ggggggtaca cgataccggg ggaccctgag
      181 gaagacacag ggcatgacga tgcccggaac gacgatagtg aggaccggta gtctccgcct
      241 ttgttttaaa aatccaagga ggtatgcatt agatgggagt agtccagact aatcagtttc
      301 ataagggtct caagatagaa tacgaaggcg gtatatggga gatcatcgaa tacagtcatt
      361 ccaagatggc gcagagaagc gccgtgatga agactaagat aagaaacgtg gtgacgggtg
      421 ccgtccagga aaagaacttc agatcaggcg attccttccg tgtccccgat acggagagaa
      481 agtccatgca gttcctctac aaagatgaaa tcggctatca tttcatggac ctcgacacct
      541 acgagcagca caccctgagc gcggcccagg tgggagaggt cgcgagcttc ataaaggacc
      601 agcaggaagt gaccgtcctt tatttcaacg gcgagcccat gggtgtcgag ctcccgacag
      661 cggtcgagct cgaggtcagg gacaccgagc ccggcatgag gggcgacacg gttacggggg
      721 ccacgaagcc cgccgttctc gagaccggag ccacgattca ggtaccatta ttcatagacg
      781 taggcgacgt gataaaggtc gatacgaggt ccggggaata tctcgagaga atgaagaaga
      841 aatagccggc tatgcatatg aattattcat tatttaatca tttattatcc ggtacgagct
      901 gttgttaaga tccggaagga aaacacattc tcctgacatg cttgactcat accctggacc
      961 cgtcctgacg ctaatggatt cagggttatg cgggaacccc cgaaaccttt tccgtaatct
     1021 taagtttata aacaataagg ctcgattggc ccgaaatgga tattgacata gagcttatgc
     1081 tgaaagctaa gtcgggggac gacggagcgt tcagcgaact gatgaggagg cattacaagg
     1141 cggtcgtgaa ttacatatac cgttatacca acgttaagga gaattccgaa gacctggccc
     1201 aggaggtgtt tttaaggatt tacaggtccc ggaagaatta caggcccgac gccaagttct
     1261 cgacgtggct ttacaagata gcgacgaacg tgagcctcac gcacgtaaag accaggaagg
     1321 tcaatcagag tctcgacgag atggaggacg agaggggcgg cataggggac cccgacatcg
     1381 acgtcgccga cgatcttata tacaggaagg agataaagga agtaatattt caggctatgg
     1441 agtctttgcc tgagagggag aaagttgcta ttatgctctg taaatacgag gggctttcat
     1501 atgaggaaac ggccgaggtg ctcgagtgca ccgtgggcgc ggtcaaggcc tacgtccaca
     1561 ggggcaggat gaagctcatc gacaagctga aaccgtactt gccgggagga ggagagtatg
     1621 ggctgtaaag acgtgagaga gatgatagcc ggctattcct cgggcgacct gcccgattcc
     1681 gggaggcagg cggtcgagtc ccatgtagcc gagtgcaaag agtgcggtct ctacctttcc
     1741 cggtacgaag gggtctggaa cctcttggac gagtggcggg agatcgagcc gcgcacggac
     1801 ttcgtatccg gtttctgggc gcaggtggca aaagaggaaa ggaagcccga caggggcatc
     1861 ctcaagaagc tattgcacgg ctgggggctc gccggggcat tcgcgactgt actcgtcgtg
     1921 ggtgtattta cattcgcgat gctcagaccc caatcgggta tcgtgggtta tgccgataac
     1981 gatacacggg acgagcagct cctccacgag ctcgacagcg tcaccgcaag tgatgcagcg
     2041 gattcggatg ctttggcaat ctacggcccc tgggacaacg gcgtcgagat catgagaata
     2101 aacggctatg gagatcagaa ttgagaacat cgctcgcatt gaccgtcatg atgtgcctct
     2161 tctccgtaag cgcgttcggt ctcgtgagcg gcgagatcca ttataagtgg tggaagaacc
     2221 ccaagatagt acaggagatg gagctcaccg aagaccaggt agacgccatc gaagatattt
     2281 tcaattctta cagggaacag ataatcgatt cccagaagga gcttaaaaaa gaggaggtcc
     2341 atctcctgga catcctcagg aagccagaat gctcgaggga cgaggtaatg cagataaccg
     2401 acaatatcga ggacttgagg gcgaacctta ctcggatcaa ggtcgacatg ctcctcaaga
     2461 tcaaaaacgt tctcacctcg gagcaggaag aaacgctcca cagcatcagg gacagatata
     2521 ggggggggag atcgcgctga gacgggtttc cgttccccgc gcggataatg tcttaccgct
     2581 ccccgcctga gggcg
//
LOCUS       DASSJH010000150         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_571409, whole genome shotgun sequence.
ACCESSION   DASSJH010000150 DASSJH010000000
VERSION     DASSJH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571409"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFJ67_03275"
     CDS             complement(<1..599)
                     /locus_tag="VFJ67_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7289436.1"
                     /translation="MSGSKSVIFYAAIILSLLILSCANGLPELNPYKEASSAPGVEWK
                     PSEKELAKSFQLEELPEIPEDLKPDSNKLALSQLVDVALINNPTTQVAWEDARAAAAA
                     WAEARGLYYPQIAGVSRYAYAKGGGSSQGTNPYQEQYGDIGLTLNYLLFDFGGREAQI
                     DAARLALINAKWNQNQAIQNVLQAVAVSFYDYIGSKALV"
     gene            complement(1131..1931)
                     /gene="trpC"
                     /locus_tag="VFJ67_03280"
     CDS             complement(1131..1931)
                     /gene="trpC"
                     /locus_tag="VFJ67_03280"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET7289437.1"
                     /translation="MAEITILDTIIENKKIEVQKSKAYRSLESLERGLRDAPDTRDFY
                     KALDDGERVRIIAEIKKASPSKGILRADFDPVKIALGYEKGGAAALSVLTDEKFFMGS
                     LSYLRAVREAVGIPLLRKDFIIDTYQIFESRLGGADALLLIVSALSRDSLEELLGLTH
                     SLGMNAVVEVHDEDELGRALDAGSRIVGINNRDLRTFEVDLGVTARLSRNIPEGVIVV
                     AESGIGSVADIKKLGEHGVRVFLIGETFMKAPDPGLELSNLISSAATV"
     gene            complement(1942..>2538)
                     /gene="trpD"
                     /locus_tag="VFJ67_03285"
     CDS             complement(1942..>2538)
                     /gene="trpD"
                     /locus_tag="VFJ67_03285"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET7289438.1"
                     /translation="LDEAGIVFLFAPLYHPAMKNVAGPRKDIGVRTIFNILGPLANPA
                     GVRHQVIGVYSEMLLDPMVKVLRNLGHKSAMLVHGSDSMDEITVTGKTVVAELRDGMV
                     RKYQFDPSDVGIKRWNASELKGGRTAKENAKILLSVLKGEEREAKRDIVLINAAAAIY
                     VSETSPDINDALGRAVESIDSGKALSKLNELAKITKTN"
BASE COUNT          573 a          673 c          656 g          636 t
ORIGIN      
        1 atgacgaggg ctttactgcc tatgtaatcg taaaacgata cggctaccgc ctggagcacg
       61 ttctgtatcg cctggttctg gttccatttg gcgttaatga gggcaagacg ggcagcgtcg
      121 atctgggctt cgcgtcctcc gaaatcgaat aggagataat tgagcgtgag tccgatgtcg
      181 ccgtactgct cctgataagg gttcgtgccc tgcgatgacc cgcccccttt tgcatatgca
      241 tacctggaga cgcccgctat ttggggatag tagagtcccc tcgcttcagc ccaggcggca
      301 gctgcggctc tcgcgtcctc ccaagccacc tgcgtggtgg ggttgtttat gagcgccacg
      361 tccacgagct gggacagcgc aagtttgttc gaatcgggtt tcaggtcttc aggaatctcc
      421 gggagctcct cgagctgaaa cgatttcgcc agctccttct ccgacggttt ccattcgacc
      481 cctggggctg atgacgcttc cttgtacggg ttcagctcgg ggaggccgtt agcgcaggac
      541 aggatcagta gggataaaat gatagccgca tagaaaatta ccgatttcga gcctgacaat
      601 tcgaatctcc gattcagatt agaaggatta aggttaatgg cggtttaagc tcccgccatg
      661 tgtcgaaaat gcctaagaac tatccgaaat aataatctcg taagagaatg attgtcaaga
      721 tacagataca cgcgtgaacg aaatagacgg ctcactttgc atcagtaaga gcgttgatcc
      781 tgtcagcgct catctttgcg tcgatcatag acgctatcaa gactacattt gccgcggatg
      841 aatatataaa cactcgaaaa agctggggca tatccgggga catattttca atcgagcccc
      901 aatacttgaa taagtaaatg aaaggttcat aagcaagtat gccgatgaca ataattagaa
      961 caaagaaaaa aagtcctttt agaatttggt tgttgtataa ctgtcccagt cccggcaaca
     1021 atccggacaa gacgagcgcg agtatggggt tttttctctt tttgttgccg gacatattct
     1081 aaaactatat atcggatcgt ggttaatgat aggattgatt tttctaatct ctagacggtg
     1141 gctgcggatg agatcagatt agaaagctcg agaccggggt caggcgcttt catgaacgtc
     1201 tcgcctataa ggaaaacacg gacgccgtgc tccccaagct tctttatgtc ggctacggag
     1261 cctattccgc tttctgctac aacgatcaca ccctcgggaa tattccgcga gagtcttgca
     1321 gtgacaccca ggtcgacctc gaaagtcctg aggtccctgt tgttgatacc gactatcctg
     1381 ctcccggcgt cgagcgccct cccgagctcg tcttcgtcgt ggacctcgac cacggcgttc
     1441 atgccgagcg agtgcgtgag tccgagaagc tcttcgagtg agtcacggga cagggcggag
     1501 actatgagca ggagggcgtc cgccccgccg agcctcgact caaatatctg atacgtgtcg
     1561 atgatgaagt cctttcgcag gagaggaatc ccgaccgcct ctctgacagc tctcaggtat
     1621 gagagactcc ccataaaaaa tttctcgtcg gtaagaacgg acagcgcagc cgctccccct
     1681 ttctcgtagc cgagggcgat cttcacgggg tcgaagtcgg ctctcaatat cccttttgag
     1741 ggagacgcct tttttatctc ggctattatc cttacgcgct ctccatcatc gagggccttg
     1801 taaaaatcac gggtatcggg cgcatcgcga agcccgcgct cgagggattc cagggaacgg
     1861 taggcctttg acttttgcac ttcgattttc ttattctcga tgatcgtgtc gagaattgtt
     1921 atttcagcca tgttccgaac tctaattggt ttttgtaatc tttgcgagct cgttgagctt
     1981 ggagagagct tttccggaat cgatcgactc cacggcccgc ccgagcgcgt cattaatatc
     2041 ggggctcgtc tcggagacgt atatcgccgc ggcggcgtta attaaaacga tgtcccgctt
     2101 ggcctccctt tcctcgccct tcaatacaga caggagtatc ttcgcattct ccttggccgt
     2161 gcggccgccc ttgagctcgc tcgcgttcca tctctttata cctacatcag aaggatcgaa
     2221 ctgatacttc ctcaccatac cgtccctgag ctcggcaacc accgttttgc ccgtgaccgt
     2281 aatctcgtcc atcgagtcag agccgtgaac caacatcgcg ctcttgtgac cgaggttcct
     2341 tagcaccttt accatgggat cgagaagcat ctccgaatag acacctatga cctgatgcct
     2401 gacgcccgca gggtttgcga ggggaccgag tatgttgaat atcgtcctta cacctatgtc
     2461 tttccgggga ccggcgacgt tcttcatggc cgggtgatag agcggggcga aaaggaatac
     2521 gatacccgct tcgtcgag
//
LOCUS       DASSJH010000151         4112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_895715, whole genome shotgun sequence.
ACCESSION   DASSJH010000151 DASSJH010000000
VERSION     DASSJH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895715"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..474
                     /locus_tag="VFJ67_03290"
     CDS             292..474
                     /locus_tag="VFJ67_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET7289439.1"
                     /translation="MGERKVMDCSKNPSNTNCTLRISGKEEEVMPAAIHHAVTAHGHK
                     DTPEFRQQLKSMLEDE"
     gene            795..2339
                     /locus_tag="VFJ67_03295"
     CDS             795..2339
                     /locus_tag="VFJ67_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR05002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat
                     NxxGxxAF-containing protein"
                     /protein_id="HET7289440.1"
                     /translation="MKRLGKPIFLLLISGFLYIMTVGGCGNGGGGGQPFPPPNALAIR
                     LAQRGQTAPDGQATFIGFLRLAGPSKGGLVTFLSLEKITEDVRRLVLYLADTSGLTEV
                     ARNGEPAPGGGIFRDFPQIIGVNDQAEVAFVGRTAESPDDEFGDTEGYYLGGEEGREL
                     LVLEGDAAPGGGAIFKLCIPGFLDCAFRYDGPNESGQITFVAALTNTDDNRALYIADS
                     SGITQLARKGDPVPGGNGVFEDFFDPAGPNENGQVAFIGRISGSSGGESDNEGIYLVD
                     ESGIIELAREGNPVPGGNGIFGNFQLLSGINESGQAALSAHLDGTSGGERDNEAIYLS
                     NDDGLTELVRKGESVPGGGGGIIDSEFYPRGPNENGQVAFGAGISSGDEDVPIDSALF
                     LAGPSGITTLIRHGDPGPGGVGKFAQFGLAGLNDSGQIIFRSRLFQGNKSAQGLYRVG
                     PGSSVTALAQQFQPAPPDGSKLYRSIEDNRYRGPSESGLAFFVAGLMNADRSYSIQDT
                     GIFLAE"
     gene            complement(2395..2634)
                     /locus_tag="VFJ67_03300"
     CDS             complement(2395..2634)
                     /locus_tag="VFJ67_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289441.1"
                     /translation="MIQYKVENITLTDDEKAFLIDLTIELEDYESDSDSVFISLSFPL
                     ANESSDLEEIKEKAIIHAKSLLKKVLLEDAQRDLF"
     gene            2875..>4112
                     /locus_tag="VFJ67_03305"
     CDS             2875..>4112
                     /locus_tag="VFJ67_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7289442.1"
                     /translation="MHDTRFINRVVLALTLCGITAGIAAHFLGKPAIAGGAWAVATVI
                     ALIPLTFVVARALYSGKLGVDIIALIAMAGSLYLGQYLAGAVIALMLSGGQVLEEYAE
                     SRARKELSALIGRTPRTVNMLDGSTIRIVAIEEVKPGDFLMVKPGEIVPVDGVVIGGS
                     AVLDESALTGESKPVERRDRDQVLSGTVNASQTPFKMRATVSAEESVYAGIIRLVEQA
                     QAARAPLVRMADRYAIFFLPATLAVSAVAWIISGDPVRALAVLVVATPCPLILAVPVA
                     IVSGLSRGARRGIIIKGGGALETLARGRVLVLDKTGTVTKGEPILSGVESLGDLSPDE
                     LLRLSASLDQVSPHVLARPIVKAAVDRGFDLSFPENVTEEFGSGISGAVDGRRVKLGR
                     MDWVLSGLEPDPRSHRLHRRS"
BASE COUNT          951 a         1115 c         1227 g          819 t
ORIGIN      
        1 tcgggcgccg tctttatgta agagacacgt tttatcaaat cacacccctg tttagcggaa
       61 ttttcttaaa gtatcgaata agatggctca atcctcaaga gcaaactgaa gaaccgttgg
      121 tgaataaata ataaccttgg aatcctgacg attcattacc cgggtagaga aatatatcaa
      181 ttggatacgg agactgactg attatcaaaa atgatcattg acagcaaatg atgacttata
      241 atagaatttg attgtaatat atcagcagcc ctcaggggga ggtagaaaac gatgggtgag
      301 agaaaagtta tggattgcag taagaacccg agcaatacca actgcactct caggataagc
      361 ggtaaagagg aagaggtgat gccggctgca atacaccacg ccgtcacagc gcacggccac
      421 aaagacacgc ccgagttcag gcagcagctg aaatccatgc tcgaagacga ataagccttc
      481 aggacagcat agaagaagct gtaacagctt gaaaacatag aatatttctt ggacttggtc
      541 gagctgacca cccggggtat aagtttttta cccacttaat ccgtaatcac agcccttgcc
      601 gggttacaga attgtcatac acataccggc agcagtatta aaagcttctt tcatttcaaa
      661 aaatataatc cttggagcaa taatcagccg tagaattaat gtaatttgaa aaggcggaat
      721 ggagcctcgt aacaggtcga cgctccctta gggagagacc ctccgggcgc ttcatccaac
      781 actggagaga tgcgatgaaa agacttggaa agcctatatt cctcttattg atttcaggat
      841 ttctctatat tatgacagtc ggcggctgcg gaaacggcgg aggcggcggc cagcccttcc
      901 ctcccccgaa cgcactcgca atcagactcg cccagagggg acagacggcg cccgacggac
      961 aggcgacttt tatcggcttc ttacggctcg ctgggccgag caaaggcggc ctagtgacct
     1021 tcctgtctct tgagaaaatc actgaggacg taaggaggct ggtcctgtac ctggccgaca
     1081 caagcgggct tacggaagtg gcgaggaacg gtgaaccggc tcccggaggg gggatattca
     1141 gggacttccc gcagattatc ggagtgaacg atcaggcaga ggtcgcgttc gtcggcagga
     1201 cagccgaatc acccgatgat gagttcggtg atacggaggg atattatctc ggcggcgaag
     1261 aagggagaga attgctcgtc ctcgaagggg acgcggctcc cggaggaggc gctatattca
     1321 agctctgcat accgggcttc ctcgactgtg ctttcagata cgacgggcct aacgagagcg
     1381 ggcagataac gttcgtcgcg gcccttacaa acacggacga caacagggca ttatacatcg
     1441 ccgattcatc gggaatcacg cagctcgcca ggaaaggaga ccccgttccg ggcgggaacg
     1501 gcgtgttcga agatttcttc gatccggcgg gtccgaatga aaacggccag gtcgcgttca
     1561 tcggacgcat atcgggctca tcaggcggcg aaagcgacaa cgagggcatt tacctcgttg
     1621 acgaaagcgg catcatagag ctcgcgcgtg aaggaaaccc ggtgccgggc ggaaacggca
     1681 tattcggtaa tttccagtta ctatcgggaa taaacgagag cgggcaggcc gcgttaagcg
     1741 cgcatctcga cggcacctca gggggcgaaa gagataatga agcgatctac ctctctaatg
     1801 atgacggatt aacggagctc gtgcgtaaag gagagtcggt tccgggcgga ggaggcggta
     1861 tcatagacag cgaattctat cccaggggtc ctaatgaaaa cggtcaggtt gcgttcggcg
     1921 cgggaataag ctcaggcgac gaggatgttc ccatcgactc ggccttattc ctcgcgggtc
     1981 catcaggcat tactacactc atccgtcacg gagatcccgg cccgggggga gtcggcaagt
     2041 tcgcgcagtt cggcctcgcg gggctgaacg actcgggtca gatcatattc aggtcgaggt
     2101 tatttcaggg aaataagtca gcccagggcc tctaccgcgt agggcccggc tcgagcgtga
     2161 ccgcgctcgc gcagcagttc cagcccgcgc cgcccgacgg ctcgaagctc taccggtcca
     2221 tcgaggacaa caggtaccgg ggcccgagcg aaagcgggct cgcgtttttc gtagccggtc
     2281 tcatgaacgc cgaccgctcg tactcgatcc aggacacagg catcttttta gcggagtgag
     2341 attgcaattg gaaagacgaa acgggatcgg gagggaatgt ctcgtcagcc ggggttaaaa
     2401 caaatccctc tgcgcgtcct cgagaagcac tttcttgagc agactctttg cgtggatgat
     2461 ggccttctcc tttatctctt cgaggtcgct cgattcgttc gcgagaggaa atgacaaaga
     2521 tatgaaaaca gagtctgagt cggattcgta atcctccagc tctatcgtca ggtctatcag
     2581 aaacgccttt tcgtcgtccg tgagagtgat gttctcgact ttgtattgaa tcattaagat
     2641 tcgcccctgc tcactctgag tatataaggc gaataaagaa tttgcaatcg aggcctcata
     2701 aagcccaatg agcagacgag ccgcggaacg gcggcgcggg ccctgaacgc gcccgtttgc
     2761 ttgctccgca tacccgctgg tgtattattc tatccgggac gttcataccc ggtcccgggc
     2821 gcggcgtgaa tctgaaaatc atagactcga cagaaccgga gttttgtaac ggctatgcat
     2881 gatacccgtt tcataaaccg ggttgtactc gccctcaccc tgtgcggcat aacagccggg
     2941 atcgcggcgc acttcctagg caagcccgcg atcgccgggg gcgcgtgggc ggtggcgacc
     3001 gtgatcgcgc tgatcccgct cacgttcgtc gtcgcgcggg ctctctactc gggcaagctc
     3061 ggcgtggaca taatcgctct catagccatg gccggctccc tctacctcgg gcaatatctc
     3121 gcaggcgcgg tgatagctct catgctctcg ggcggtcagg tgctagagga gtacgccgaa
     3181 tctagggctc gaaaagagct atccgcatta atagggcgca ctccccgaac cgtcaacatg
     3241 ctcgacggca gcacgatccg gattgtggcg atcgaggaag tgaagccggg cgacttcctc
     3301 atggtcaagc ccggtgagat cgtccccgtg gacggcgtcg taatcggcgg ctccgcggtg
     3361 ctcgacgaat ccgccctcac cggagaatcg aaaccagtcg aacgcaggga cagggatcag
     3421 gtgctcagcg gcacggtgaa cgcttcccaa acccctttca agatgcgggc gaccgtgagc
     3481 gcggaggaga gcgtgtacgc ggggataatc aggctcgtcg agcaggcgca ggcagccagg
     3541 gccccgctcg tgaggatggc cgacaggtac gcgatatttt ttctcccggc gaccctcgcc
     3601 gtctcggcgg tcgcatggat aatctcgggc gacccggtgc gggccctggc ggtgcttgtc
     3661 gtcgccaccc cctgccccct catattggcg gtgcccgtcg ctatagtgtc cggcctctcg
     3721 cggggcgcgc ggcgcggcat catcatcaag ggcggcgggg ctctcgaaac cctcgcccgc
     3781 ggcagggtcc tcgtgctcga taaaacaggg actgtgacga agggggagcc gatcctcagc
     3841 ggcgtggaga gcctcgggga cttaagcccc gatgagcttc tcaggctctc ggcgtccctc
     3901 gatcaggtct cccctcacgt gctcgcgcgg cccatcgtaa aagcggccgt ggaccgggga
     3961 ttcgatctca gcttccctga aaacgtgacg gaagagttcg gctcgggtat aagcggcgcg
     4021 gtcgacggga ggcgcgtgaa gctgggcagg atggactggg tgctgagcgg gctcgagccc
     4081 gatccacggt cgcacaggct ccacaggcgg tc
//
LOCUS       DASSJH010000152         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_466353, whole genome shotgun sequence.
ACCESSION   DASSJH010000152 DASSJH010000000
VERSION     DASSJH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466353"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFJ67_03310"
     CDS             <1..243
                     /locus_tag="VFJ67_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HET7289443.1"
                     /translation="KGCEKCFHMGYSGRTAIFEMLLIDDDIRNLTLSQVDSTKIKRKA
                     MEHGMTTLRMDGAEKVARGLTSVDEVMRVTEEESII"
     gene            300..1505
                     /gene="gspF"
                     /locus_tag="VFJ67_03315"
     CDS             300..1505
                     /gene="gspF"
                     /locus_tag="VFJ67_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HET7289444.1"
                     /translation="MPVYKYKAIDETGRNIQGIVDAESPRGATEKLKRQGVFLSSLSE
                     VKQGKARSFIPFRGVNISELAITTRQFSTLISAGLPLEASLVALSEQTEDARLGQILT
                     EVRDRVSEGSSLANALGEHRNVFSDLYINIVRAGEASGTLDIVLLRLADFLEKQAALT
                     SKVKSALIYPIFMFFIGGGVLFFTMTYVIPRIAKIFEDSQKALPLMTVILINVSGFFS
                     SNILLLLILLPILLFAAYRFNKTERGSMLFDRISLKLPVFGKINSMVVISRFTRTLGT
                     LLASGIPLLDALKIGEAVLGNQVYSKTLEEVRSNVREGTSLAKPLRDSGVFPPLVTRM
                     IAVGEQTGEMEEMLTKVADIYDQQVETMVSTLTSLLEPVMILIIGAVMGFIVFAVLLP
                     IFNLTSTIS"
     gene            1521..1949
                     /gene="gspG"
                     /locus_tag="VFJ67_03320"
     CDS             1521..1949
                     /gene="gspG"
                     /locus_tag="VFJ67_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HET7289445.1"
                     /translation="MRSEGGFTLIEIMVVLLIIAGLAYIVGTNVIGRFGEAKKEETKI
                     QIKNLESALKLFKLDNGFYPETQQGLNALIQMPTTGRQPCCYAKDGYLEGGQVPKDGW
                     KNEFVYIGPDQTGDGTYEIISLGEDAVQQSEDDITSRSIQ"
     gene            1946..2479
                     /locus_tag="VFJ67_03325"
     CDS             1946..2479
                     /locus_tag="VFJ67_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7289446.1"
                     /translation="MRDRNGFTILELMVVVLLISAFVFIAVPKIKSGTEINIKSASTN
                     LTATIRYLYNEAAFKKNIYRLVFDIDRDEYWVEVLDNNEYVVSNEYLHKKRTLPAGVH
                     FKDVVTERTQSRSRLDNQKDFILFLPTGFVEPAVIHLVTDSEDYYTLETKPYTGGTRV
                     YDEYVELLNNDYTGNNR"
     gene            2505..2885
                     /locus_tag="VFJ67_03330"
     CDS             2505..2885
                     /locus_tag="VFJ67_03330"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7289447.1"
                     /translation="MPAAASGKSGFTLLEVIIAVAIIGSSLAILLGAVNKNLVLASQS
                     KNITLASFLAQRKLGEVEVEGFPEIGNQEGVFEEHPEFRWYLSVQPYNIEQLGTEIRI
                     VILTVSWDEGSREFTVATAISDHG"
     gene            2857..>3369
                     /locus_tag="VFJ67_03335"
     CDS             2857..>3369
                     /locus_tag="VFJ67_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7289448.1"
                     /translation="MRRRYQTTDSGFTLIEVLLAVFIGSIVLTVLYASFFQINKAKAR
                     IEEELDLYHESRIIMSKITKDLATAFPRGLVNSTKTNIEAPFFLGADEGGKSRLSFTS
                     LSRTPTQDARESDQTEISYYLEPIQDSDLFALMRRDDPTIESDTGATQYPLSERVVSF
                     NLTYLAKIPEN"
BASE COUNT          914 a          850 c          917 g          688 t
ORIGIN      
        1 aagggttgcg aaaaatgctt ccatatgggg tatagcggca ggacggctat cttcgagatg
       61 ctgctgatcg acgacgatat caggaacctg accctcagtc aggtcgattc gacaaagatc
      121 aagagaaagg caatggagca cggaatgacc acgctcagga tggacggggc tgagaaagtg
      181 gcaaggggtt tgacctcggt cgacgaggtc atgagggtta ctgaagagga aagcataatc
      241 tagccagcct aacgtgatca ccccaatttt aacaacagaa tcggatatta tataaacata
      301 tgcccgtata caagtataaa gccatcgacg agaccggacg taacatacag ggaatcgtcg
      361 acgcggaatc cccgaggggc gcgacggaga aactcaagcg ccagggggtc ttcctatcgt
      421 ctctgagcga ggtcaagcag ggaaaggcca ggagctttat tcccttccgg ggcgtaaaca
      481 tctcggagct cgcgattacg acgagacaat tctcgacgct tatatcggcc ggactccccc
      541 tcgaagcgtc tctggtcgcg ctttccgaac agacggaaga cgccaggctc ggacagatac
      601 tgacagaggt cagggacagg gtcagcgaag gcagctcgct ggcaaacgcc ctcggtgaac
      661 accgaaacgt attctccgac ctctatataa acatagtaag ggccggggaa gcgagcggta
      721 cgctcgacat agttctccta aggctcgctg acttcctgga aaagcaggcc gccctcacat
      781 ccaaagtgaa aagcgcgctt atatatccca tattcatgtt tttcatcggc ggcggggtgt
      841 tgttcttcac catgacatac gtaataccga ggatcgcgaa aatattcgag gacagccaga
      901 aggccctgcc tctcatgacc gtaatactga taaatgtaag cggctttttc agcagcaaca
      961 tactgctgct tctcattttg ctgccaatac ttttattcgc cgcatacagg tttaataaaa
     1021 cggagcgggg cagtatgctg ttcgaccgca tctctctcaa gctgccagta ttcggaaaga
     1081 taaattcgat ggtcgtgata tccaggttca cccgcacgct cgggacactg ctagcgagcg
     1141 gtataccgct cctcgacgcc cttaaaatag gtgaggcggt cctgggcaac caggtctaca
     1201 gcaaaacgct tgaagaggta aggtccaacg tcagggaagg gacgagcctc gcaaagccgc
     1261 tcagggacag cggagtcttc ccgcctctcg taacgcgaat gatagccgtc ggcgaacaga
     1321 caggcgaaat ggaagagatg cttacgaagg tggccgatat ctacgaccag caggtcgaga
     1381 cgatggtatc tacgcttact tcactcctcg aacccgttat gatattaatt ataggcgccg
     1441 tgatgggatt catagtgttc gcggtcctct taccgatatt caatttgaca tcgactataa
     1501 gttaaaagga ggtatgaaat atgaggtctg aaggcggttt cacgcttatt gaaattatgg
     1561 tcgtgctcct gatcatcgcg gggctcgcgt acatagtggg cacgaacgtg atcgggaggt
     1621 tcggggaggc caagaaagag gagaccaaga tccagataaa gaaccttgag tccgcactca
     1681 agcttttcaa gctggataac ggtttctacc ccgagacgca gcagggtctt aacgccctca
     1741 tacagatgcc gaccaccggg agacaaccct gctgctacgc aaaagacgga tacctggaag
     1801 gtggacaggt ccccaaggac ggctggaaaa acgagttcgt atatatcggt cccgaccaga
     1861 cgggggacgg aacctatgag ataatctcac tcggcgagga cgccgtacaa caaagcgagg
     1921 acgacattac gagccgcagc atacaatgag agacaggaac ggttttacga tactggagct
     1981 gatggtcgtg gtattactca tcagcgcttt tgtattcata gccgtgccca agattaaaag
     2041 cggtaccgag atcaacataa agagcgcctc gacgaacctg accgcaacca taaggtatct
     2101 gtataacgag gcggcattta aaaaaaacat atacaggctc gttttcgata tcgacaggga
     2161 cgagtactgg gtcgaagtgc tcgacaacaa cgagtacgta gtgtcgaatg aatacctgca
     2221 caagaaaaga acgctgcccg cgggcgtcca cttcaaggac gtggttaccg agcgcacaca
     2281 aagcaggagc aggctcgata accagaagga ttttatcctg ttcctgccca cgggtttcgt
     2341 tgaaccggcg gtcatccacc tcgtgaccga cagcgaggat tattatacgc tggaaaccaa
     2401 gccctacacc ggaggaacca gggtgtacga cgaatacgta gaactgttga ataatgatta
     2461 taccgggaac aatcgctaaa caaacctgga acgaagggac gcgaatgccg gcagcagcat
     2521 ccgggaaatc cgggttcacg cttctggaag tgataatcgc agtcgccatt atcggctctt
     2581 ctctggcaat actgttaggc gccgtcaaca agaatctcgt actggcgtcg cagtcgaaaa
     2641 acatcactct cgcttctttc ctggcacaga ggaagctcgg cgaggtggag gtcgaggggt
     2701 ttcccgaaat cggcaatcag gagggcgttt tcgaggagca cccggagttc agatggtatt
     2761 tatccgtaca accttacaat atagagcagc tcgggaccga gatcaggata gtgatactca
     2821 cggtatcatg ggacgaaggc agcagggagt ttaccgttgc gacggcgata tcagaccacg
     2881 gatagcgggt tcacgctcat cgaagtgctt ctcgccgtat ttatcgggtc gatagtgctc
     2941 accgttcttt acgcttcctt cttccagata aacaaggcga aggcccgtat cgaggaagaa
     3001 ctcgatctgt atcatgagtc gaggataatc atgtcgaaaa taaccaagga cctcgcgacc
     3061 gcgttccccc gcgggctcgt gaattcgact aagaccaata tcgaggcccc gttcttttta
     3121 ggcgcggacg agggcgggaa gagcagattg agcttcacat cgttgtcgcg gacgcccacg
     3181 caagacgcaa gagagtccga ccagaccgag ataagctatt atctcgagcc gatacaggat
     3241 tccgacctct tcgccctcat gaggagggac gacccgacca tcgaatccga taccggggcc
     3301 actcagtatc ccctgtcgga gagggtcgtg agtttcaacc tcacatattt ggcgaagata
     3361 cccgagaac
//
LOCUS       DASSJH010000153         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_250134, whole genome shotgun sequence.
ACCESSION   DASSJH010000153 DASSJH010000000
VERSION     DASSJH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250134"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1996
                     /locus_tag="VFJ67_03340"
     CDS             <1..1996
                     /locus_tag="VFJ67_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7289449.1"
                     /translation="VARSEALFRAVFEGAGIGIALVGQDGRPITINPALQKMLGYSEE
                     EIKSMVFTEFTHPEDAQKDWDLFSELIEGKIDHYQMEKRYFHKNGLIVHGLLSVSLIK
                     GLEGSEPFVLATVADITERVTGEEELTVLNKLMQAVHRFLDLEEVYDVALETVVGTDI
                     VDMAMIYLVDEEKKEAVLQAHRNLPDSYVQKAGRIPHPKGNTWRVIGSGAIINIENVQ
                     NDPNMGPAGRALGEHSALGIPIFIKDKVIGVLWFFSIKERKFSGKEIRFLTTLGDQIA
                     IAVAKAQMMKEIEKAQEQLVQAEKMASIGRLISSIAHEINNPLTPIVGYSQRLLEKND
                     LGKHEKESLQIIFNSAQRVVRIIEKLLTFSRESLPVRSYEDINSLIEQTLEFREYQYE
                     LENVEIVRKLDPGLPKTMLDPGQIQQVLMNLLLNAEQAIAESRSSGRIEIETKLKNSS
                     CIEITISDDGPGIADEIRDKVFDPFFTTKEQGKGTGLGLSVSYGIIKKHGGEIFIQEG
                     VREGAGFVIDLPVTTPHGEYGGTENTETEDRIMEMKNKRVLIVEDEVIMISLLKTVLE
                     EEGYIADIASCGREAIHNRDLGQYDLIVCDIRLPDINGMELFKELKKRYPDVADRVFF
                     ITGDTSSKTKKFLDKSGNPYLLKPFRIDKFKERVNEVLLG"
     gene            complement(2035..2517)
                     /locus_tag="VFJ67_03345"
     CDS             complement(2035..2517)
                     /locus_tag="VFJ67_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7289450.1"
                     /translation="MGAKKQKYLLKAGEIKKMQKKFSHPWNPKSELVGARLGVKTGLV
                     RTGVNIATIPPGKESFVYHSHTFEEEWIYVLTGSGVAEIDGKEYKVGPGDFMGFPTPG
                     VAHNLRNDGKEDLVYLVGGESREHEIAEYPRLGRKMIRTGEKVEVLKVSDVKDFKFWE
                     "
     gene            2823..>3337
                     /locus_tag="VFJ67_03350"
     CDS             2823..>3337
                     /locus_tag="VFJ67_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289451.1"
                     /translation="MNPKERLILGTVLPFLFLLNVAGCDVEFGSGDSGGGGTSTTDAE
                     LSGEVYEVNSGDVEGITVELEDENSGYTYEDTTDSGGGFKITGGFCGTSTVTLTDAEG
                     TRLAVFSLDIIPGSDTDIGNLTVSSGTVTFNDEIITEFDGYVTENDCDGTSGTLTVEV
                     DNDCDTVDVFVT"
BASE COUNT          876 a          871 c          954 g          636 t
ORIGIN      
        1 ggtcgcccgc agcgaggcgc ttttccgggc ggtattcgag ggagcgggca ttggaatagc
       61 cctcgtgggc caggacgggc gcccgatcac gatcaacccg gcgctgcaga agatgctcgg
      121 ttacagcgaa gaggaaatca agtcgatggt gtttacggaa ttcacgcacc cggaagacgc
      181 acaaaaggac tgggacctct tcagtgagct gatcgaaggc aagatcgatc attatcagat
      241 ggagaaacgg tacttccata agaacggact gatcgtgcac ggcctgctga gcgtctctct
      301 tatcaaaggt ctcgaagggt cggagccttt cgtcctcgcc acggtcgcag atattacgga
      361 aagagtaacg ggggaagaag agctgacggt actgaacaag ctcatgcagg cggtgcacag
      421 gttcctcgac ctggaagaag tgtatgacgt cgcgcttgaa accgtagtcg ggacggacat
      481 cgtggacatg gcgatgatct acctggtcga tgaagaaaag aaggaggcgg tactgcaggc
      541 gcacaggaac ctgcccgatt cttacgtgca gaaagcgggc aggatacccc atcccaaagg
      601 gaacacgtgg agggtgatcg ggtcgggtgc gatcatcaac atcgaaaacg tccagaatga
      661 tcccaacatg ggaccggccg gcagggcgct cggcgagcat agcgcattag gaataccgat
      721 attcattaaa gataaagtaa taggagtact ctggtttttc agcattaagg agcgcaaatt
      781 cagcgggaaa gaaatacggt tcctcacgac tctcggcgac cagatcgcga tagccgtagc
      841 gaaagcgcag atgatgaagg agatcgaaaa ggctcaggag cagctcgtcc aggccgagaa
      901 gatggcttcc ataggaaggc tgatatctag catagcgcac gagataaaca atccgctcac
      961 tcccatcgtc gggtattcgc aaagactcct cgaaaaaaac gacctcggca agcatgagaa
     1021 ggaatcactg cagataatat tcaattcggc ccagagggtc gtcaggatca tagagaagct
     1081 gcttacattt tcgagagaat ctttacctgt cagaagttat gaggatataa acagcctgat
     1141 agagcagact ctcgagttca gggaatatca atacgagctg gagaacgtcg aaatcgtcag
     1201 gaaactcgac cccgggcttc ccaaaacgat gcttgacccc ggccagatcc agcaggtgct
     1261 catgaatttg ctgctgaacg ccgaacaggc aatcgcagag tcgcgcagca gcggaaggat
     1321 agagatagaa acgaagctga agaacagcag ctgtatcgag ataaccatat ccgatgacgg
     1381 gccgggcatt gccgatgaaa tcagggacaa ggtattcgac cccttcttca cgaccaaaga
     1441 gcaggggaaa gggacggggc tcggtctttc cgtcagctac gggatcatca agaagcacgg
     1501 gggagagata ttcatacagg agggagtaag ggaaggagcc ggattcgtga tcgacctgcc
     1561 ggtcacgact ccgcacggcg agtacggcgg gacggagaac acggagaccg aagaccgcat
     1621 tatggaaatg aaaaataaaa gagtgctgat cgtcgaagac gaagtaataa tgatcagctt
     1681 attaaaaacg gtcctcgaag aggagggata tatcgccgac atagcctcat gcggcaggga
     1741 agccatacac aaccgagatc tagggcaata cgacctgatc gtgtgcgata taaggctgcc
     1801 ggacataaac ggaatggaat tatttaaaga gcttaaaaaa cgctatcccg atgttgcgga
     1861 cagggtattt ttcattacag gcgataccag cagcaaaacg aagaaattcc tggataaatc
     1921 cggcaacccg tacctgctga aacctttcag gatagacaaa ttcaaagagc gggtaaacga
     1981 ggtactcttg ggctgaacgg acgcagcagc cggatgatca gccgtcctcc cccctcattc
     2041 ccagaacttg aagtccttta cgtccgagac cttgagcacc tctaccttct ccccggtcct
     2101 tatcatcttc cgccctagcc tcgggtactc ggcgatctcg tgctcccggc tctcgccgcc
     2161 gacgaggtaa accaggtcct ccttcccgtc gttccgcaag ttgtgcgcga cccccggcgt
     2221 ggggaacccc atgaagtccc cgggcccgac cttgtattcc ttcccgtcta tttccgcgac
     2281 gcccgacccc gtgagcacgt atatccactc ctcctcgaac gtgtgcgagt ggtagacgaa
     2341 cgattccttc cccggcggta ttgtcgcaat gttaacgcca gtcctcacga gccccgtttt
     2401 cacgccgagc cgcgccccga ccagctccga tttgggattc caagggtgtg agaacttctt
     2461 ctgcatcttt tttatctccc ccgccttgag tagatacttc tgcttcttcg cgcccatgcg
     2521 agcctcccgt tgtgatttgt atgattagta tatgagaaga ggggcgcgga ataaaggggc
     2581 gctgttcagg gacgaaggcc gtgcgtgtga catcgaaata ttttttaccg accgaaggaa
     2641 atccccgtgc ttatacgttc aattaatagg agccgcggtt cgaagctcct gacaccagag
     2701 taaagacaat tgatcggaac gggggagccc gccggcccat accctgaatc ctgaagctga
     2761 aacatgaacg gcggactccc tcactcaagg acggccgtcc ggccgcacag cgggagaaca
     2821 ttatgaatcc gaaagagaga ttgatcctcg ggacagtact acctttttta ttccttctca
     2881 acgtagcggg ctgcgacgtg gaattcggct cgggggacag cggaggaggc ggtacgagca
     2941 ccacagacgc cgagctcagc ggcgaggttt acgaggtaaa ctcgggcgac gtcgagggca
     3001 taacggtcga gctcgaggac gagaactcgg gctacacgta cgaggacacg accgacagcg
     3061 gaggcggttt caagattacg ggaggcttct gcgggacgag cacggtgacc ctgacggatg
     3121 cggaaggcac gcggctcgcc gtcttctcgc tcgacataat ccccggctcg gacacggaca
     3181 tcggaaacct gaccgtatct tcaggcacgg tcacgttcaa cgacgaaatc ataacggagt
     3241 tcgacggata cgtcacggaa aacgactgcg acggcacttc cggcacgctg accgtggagg
     3301 tggacaacga ctgcgatacg gttgacgtgt tcgtcac
//
LOCUS       DASSJH010000154         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_141934, whole genome shotgun sequence.
ACCESSION   DASSJH010000154 DASSJH010000000
VERSION     DASSJH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141934"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFJ67_03355"
     CDS             <1..269
                     /locus_tag="VFJ67_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HET7289452.1"
                     /translation="SLMEGVARGELDGSGLWNLSKDGEYTTARYDWQVETTKPWMNLI
                     APLARPLFKWNHDIVMKWGAQGLARRLDAVVIEERESREYNPLP"
     gene            271..699
                     /locus_tag="VFJ67_03360"
     CDS             271..699
                     /locus_tag="VFJ67_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7289453.1"
                     /translation="MNTVQKEITLSPKERGFHLVTREIAEHVKELSEYSVGIAHIFIC
                     HTSASLTVNENADPDVREDFETHFNKTVPENAPYYVHTLEGPDDMPAHIKASILGSSV
                     TLPVKNGRFNLGTWQGIYLCEHRNHSGPRKLIVTIIGEKK"
     gene            complement(715..1059)
                     /locus_tag="VFJ67_03365"
     CDS             complement(715..1059)
                     /locus_tag="VFJ67_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007983031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HET7289454.1"
                     /translation="MNLKEAFDSIIERLNGGASVRAVYGEPVEAQGKTIIPVAKVMYG
                     FGGGFGETGKDKKNGTDKEGGGAGGGVRAKPVGVIEITDEDTRFVPCADGRKYVALLM
                     LGFVVGFFIGRR"
     gene            1241..>2140
                     /locus_tag="VFJ67_03370"
     CDS             1241..>2140
                     /locus_tag="VFJ67_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET7289455.1"
                     /translation="MAEITRSKTRVKGFGNEEMDFQLIRQLGSASFGGASAGQCLSAA
                     ARIKDGDPGSWVNEFAKLAEWQMKDAHKRASKGRTTSARDQFLKSSNSFRAAEYYTSP
                     LDPRHRELGLKARDCFIEAMKYVWHTFEEEMLTYKNIELPVYIMTPGLEAQRRKTLLI
                     VSGFDGTLEEEYLMHGYPALERGYNVVHYAGPGQMDLYRFFTHTHFEPDFENVTKTVI
                     DFIIDRPEIETSKLALMGISVGGYFSARSAAHEPRLKALIPNSPILDLHDYLSAFIGY
                     DPAEIPDDQNFTVEDIPNIPDDLF"
BASE COUNT          558 a          591 c          566 g          425 t
ORIGIN      
        1 tgtcgctcat ggagggcgtt gccagagggg aattggacgg cagcggctta tggaatttat
       61 cgaaagacgg cgaatatacg acagcaagat acgactggca ggtagagacc accaaaccct
      121 ggatgaacct gatagctcca ctcgcgaggc cgctcttcaa atggaatcac gacatagtga
      181 tgaagtgggg cgcacagggg ttagcccgga ggctagacgc ggtcgtgatt gaagagaggg
      241 aaagccggga gtataatccc ctaccctgat atgaacaccg ttcagaaaga gataactctt
      301 tctccaaaag agcgagggtt ccatctcgtc acccgagaaa tagccgagca cgtgaaggag
      361 ctgtcggaat actctgtcgg catcgcgcat attttcatat gccacacatc cgcctcgctc
      421 actgtcaacg aaaacgccga ccccgacgtg agggaagact tcgaaacaca cttcaataaa
      481 accgtccccg aaaacgcccc atactacgtg catacgctcg agggcccgga cgatatgccc
      541 gcccacataa aggcgtctat actagggagc tcggtgaccc tgcccgtgaa gaacgggaga
      601 tttaatctcg gaacgtggca gggtatatac ctctgcgaac accggaacca cagcggtccg
      661 aggaaattga tcgttacgat tattggagaa aagaagtgaa gcgagccgcc gggctcatct
      721 ccttccgata aagaatccta caacaaaacc gagcatgagg agcgccacgt actttctccc
      781 gtccgcgcac gggacgaagc gggtgtcctc atcggtaatt tcaataaccc cgacgggctt
      841 tgcccgaacg cccccacctg ctcctccgcc ctctttgtcg gtcccgtttt tcttgtcctt
      901 ccccgtctcc ccgaatcccc cgccgaagcc gtacatgacc ttggcaacgg ggataatcgt
      961 cttcccctgc gcctctacag gctctccgta aacggccctg acagaggctc cgccgttcag
     1021 acgctcgatt atggaatcga aggcttcctt caagttcata tctaaagcat aacaccataa
     1081 tcgggaaata tctccctcga tcatcgtacg cgttcgatga gagagcgtcc ggctcaaaat
     1141 gtcccgcgag ggagttgtta cgcagcaggc aagaaagtat agttaatagc gttttatata
     1201 tgtccgaatc aaaaaacaca gaccccggag taatatagga atggccgaaa taacacggag
     1261 caaaacaaga gtcaaaggat tcgggaacga ggaaatggac tttcagctca tacggcaatt
     1321 gggctccgcg tcgttcggag gagcgtcggc cggccaatgc ctctcggcag cggcgcgcat
     1381 taaagacggc gaccccggga gctgggtcaa tgaattcgcg aagctcgccg aatggcagat
     1441 gaaagacgct cacaaacggg cgtctaaggg acgcacgacg agtgcacgcg atcagttttt
     1501 aaagtcgtct aattccttcc gcgcggctga atattacacg agccccctag accccaggca
     1561 cagagagctc ggtttaaagg cccgggactg cttcatcgaa gccatgaaat acgtctggca
     1621 taccttcgag gaagagatgc tgacttataa aaatatcgag ctcccggtgt atatcatgac
     1681 gccgggtctt gaggcgcaga ggaggaaaac gctcctcata gtgagcggct ttgacggtac
     1741 gctcgaagag gagtatctga tgcacgggta ccccgcgctt gagagggggt acaacgtcgt
     1801 acactatgcc gggcccggtc agatggacct ttacaggttc tttacgcaca cgcacttcga
     1861 gcccgatttc gagaacgtca cgaaaactgt aatagacttt atcatcgaca ggccggagat
     1921 agagacttcg aagctcgccc tcatgggtat aagcgtggga ggatacttct cggccaggtc
     1981 cgcggcgcac gagccgaggc taaaggccct catcccgaac tcgcccatac ttgaccttca
     2041 cgattacctg agcgcgttta taggatacga tcccgccgaa attcccgacg accagaattt
     2101 caccgtggag gacattccga atattccgga cgatttattc
//
LOCUS       DASSJH010000155         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1004882, whole genome shotgun sequence.
ACCESSION   DASSJH010000155 DASSJH010000000
VERSION     DASSJH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004882"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            755..1636
                     /locus_tag="VFJ67_03375"
     CDS             755..1636
                     /locus_tag="VFJ67_03375"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7289456.1"
                     /translation="MILLTGATGTTGRRVARRLTKWGYRFSALVRDPDKASGLKSSKV
                     DLIKGDFGKPDSLKRAMEGIEHAFLLPPNTKDQFKLERNFIDAARESGVKYLVKYSAI
                     GANPGSPSRLLKWHGEAEEYLKQSGIRYSIIRPNIFMQNFTNYYARQVRDEKQFRLPI
                     GDARCGWVDVRDIGRLIAKILTENGHQNNILTVTGPESLNCNEIADILSNAVGKKIEY
                     VDVKPKEFRKEMMESGVPETLAEAVSELYKLVSDGLCDVVTDTFYEITQKQPRTFDEF
                     VDDNLSLFFKKKSKSKK"
     gene            complement(1938..2510)
                     /locus_tag="VFJ67_03380"
     CDS             complement(1938..2510)
                     /locus_tag="VFJ67_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289457.1"
                     /translation="MARIIFALLAVMMLAVFTTGSFAQQGTTEGNPYSFLFVQTATSM
                     SYKDGKLTLKNVSPSMIFFADRPERMAGHFPTTHFLKMWDDGKDRFKNDPPNANLSII
                     GDREGTTDTDVVVELTNPSLSEKGDLTYDVEVLDGTMPAEGGLTSVFIDWWVAPDGAI
                     CHYNWYTGYRVCRFPGPYYWGPYRRPYWWY"
BASE COUNT          732 a          788 c          647 g          702 t
ORIGIN      
        1 atattcgggg cagtcagtta gccgttatat tttatatata ccttatatca tctcgtccgg
       61 tttcgaggcg tattaacaaa cctaacgtga cattcccgcc cccttccctc gaatcggctt
      121 taacgccggc cttcttcccg actcgcccgg aacccggggt cggagtcacg tcattctccc
      181 ggtaattaat tggatcagat tagattattc cgggatctgg agtattcacc gtctatgatt
      241 acagcgggca gggacaccga cgaatattac gggccttctt atacggagat ttcatcaaaa
      301 aagagatgac tttttatcac taaagttatc ctttcttaat attatcttct aactaattat
      361 catactcgtt cataggctct taatatttca aaaaatactt gacacagtat catctctgat
      421 ctaataatta cattgttact cttttcaggt tctttacagg ctttaccgcc tgattattaa
      481 acaaaaggga caggtagtta taaattgggg acaagcggac tgaatgcggc tcgcgtattc
      541 aatcaagctg catatcgcta taaagctcca cccttatacc atcttgagca tgtattacca
      601 cactccgttt ccttctaccg ccagaaaggg ggggacggag tatcttttat cagtctccga
      661 tagcgtcctg acctcatttc ccccatcccc ttcccaaaca cctcgtttgt ctgatagact
      721 aatgtcatag ccagaagcat ccggggtgaa tcatatgata ttgctgaccg gcgctacggg
      781 cacgacaggc cgtagggtcg ccaggcgttt gacgaagtgg ggctacaggt tcagcgccct
      841 cgtcagagat ccggataaag catccggtct caagtcatcc aaagtagatc tcataaaagg
      901 ggatttcgga aaaccggatt ctctcaagag agcgatggaa gggatagaac atgccttttt
      961 gctcccgccc aatacgaagg accagttcaa gctcgaaagg aatttcatcg acgccgcccg
     1021 cgaatccggc gtgaagtatc tcgtcaaata ctcagccatc ggagccaacc ccggctcccc
     1081 ttcccgcctc ctgaagtggc acggcgaggc cgaggaatac ctgaagcagt cgggcataag
     1141 gtactcgata atccgaccca acatattcat gcagaacttc acgaactact acgcccgtca
     1201 ggtaagggat gagaagcagt tcaggctccc catcggagac gcgcgctgcg gatgggtgga
     1261 tgtgagggac atcggcaggc tgatcgcgaa gattctgacg gagaacggcc atcagaacaa
     1321 tatcctcaca gtcaccgggc ccgagtctct caactgcaat gaaatagctg atatcctctc
     1381 gaacgccgtc ggcaagaaga tagagtacgt cgatgtgaag ccgaaggaat tcaggaaaga
     1441 gatgatggaa tcaggcgtgc ccgagactct tgccgaagcc gtgtccgagc tatacaagct
     1501 agtgagcgac ggactctgcg atgtcgtcac cgacacgttc tacgagataa cgcagaaaca
     1561 gccccgcacc ttcgacgaat tcgtcgacga caacctttcc ctcttcttca aaaagaaatc
     1621 caaatcgaaa aaatgatctg atactactac ccttacggta ttagaccagc aacgaaacca
     1681 atcccgttca cccagagctt gtcgaagggt tgaacgggta tcactcccgt agtgctgggg
     1741 tggaattgaa agatattcac tgtaataaga tattttagcc aattctattc tgtcttttca
     1801 taaatcacac tacgtgtgca tcaaggaaaa gaagaaaata aacatttcct gccctttcca
     1861 caacttcgcg caggtcggct caatgccgca ccaagcgtct tgtaattaaa caaccgtggg
     1921 taaagctcag tcccttctca ataccaccag tagggcctgc ggtaaggtcc ccagtagtat
     1981 ggccccggga accggcaaac cctgtatccc gtgtaccagt tgtaatggca tattgcgccg
     2041 tccggcgcga cccaccagtc tatgaagacc gatgtgagcc cgccctccgc cggcatcgtc
     2101 ccgtcgagca cctcgacgtc gtacgtcagg tcccccttct cgctgagcga ggggttagtc
     2161 agctcgacca ccacgtccgt atcggtcgtc ccttccctat ctccgatgat cgacaggttc
     2221 gcgttcggcg ggtcgttctt gaatctgtcc ttcccgtcgt cccacatctt gaggaagtgc
     2281 gtcgtcggga aatgcccggc catcctctcg ggcctgtccg cgaagaatat catcgacggt
     2341 gatacgtttt taagcgtcag cttcccgtcc ttatacgaca tcgacgtcgc cgtctgaacg
     2401 aacaggaagc tgtacgggtt cccttccgtc gtcccctgct gagcgaagga accggtcgta
     2461 aacacagcca acatcattac cgctaacaat gcgaatatta ttcttgccat tttgctcctc
     2521 tcctgatgtg ttatcggatc aagtccgttc ctaatattat cacaattcct gccggggata
     2581 taaacggaat ttttagccga attccgaacc atcgcagcat aaaccagcga ctgcgacaat
     2641 ttcgttcgtc cgcagtgacc ccgcctcccg ttcgtcctga gcgcacacgt agtgtgcagt
     2701 cgaaggatat attatttttc attgcaacga ttgcgatgaa taagagcaat cggtcgccct
     2761 tagctaattg caagtaggcc acataatcat tgtacatgtg caaaacaatc caaattacgc
     2821 agtgaaacgc agcgcggcaa gcccatcctt ttttctctta tttccctcc
//
LOCUS       DASSJH010000156         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_466967, whole genome shotgun sequence.
ACCESSION   DASSJH010000156 DASSJH010000000
VERSION     DASSJH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466967"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFJ67_03385"
     CDS             complement(<1..443)
                     /locus_tag="VFJ67_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7289458.1"
                     /translation="MTKKINFEEIFDSLPESIIIIDKELRVLDINDNAESTFRISREK
                     ARGQRSKVFMPEEIDRVALMAMGEGRTVIGDEIKPTLRSGERISIQAVASPIFTQEGD
                     LKGAILQIKDKSGTNFLSSKSIQEISAAKFENLVLGLAHELKNPL"
     gene            complement(547..1848)
                     /locus_tag="VFJ67_03390"
     CDS             complement(547..1848)
                     /locus_tag="VFJ67_03390"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET7289459.1"
                     /translation="MSVVNVGLIGAGTVGCGVIKVLNKNGDIIEKRTGIRLNLKKIAD
                     IDPDRKRPVKIPKGKLIRDARELIEDGSIHIIIELVGGTGIARDFVMSAIRNGKHVVT
                     ANKALLAHYGREIFSAAVEKGVDIGFEASVGGGIPIIKVTQESFAANNILSIHGIING
                     TSNYILSRMTEEGSEFSDVLKEAQKEGYAEADPSFDVDGIDAAHKLSILIMLSYGIFI
                     DFGKIHVEGIRGITPLDIAYADELGYKIKLLAVAKSKDKGLEAGVYPALVRKETQLAD
                     VSGAFNAIYLVGDAVGPAMLYGKGAGMMPTASAVVSDVLQIAKDLSLGNTHSSPPRFY
                     GGKGSSPLIKMSDTTNKYYLRFQAEDRPGVLGRITGSLGKHGISIESVIQKGRHLGGG
                     DVPVVIMTHESLEKDLLSSLREIDRFPTIRAKTTFIRIEDL"
     gene            1953..2028
                     /locus_tag="VFJ67_03395"
     tRNA            1953..2028
                     /locus_tag="VFJ67_03395"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1986..1988,aa:Ala,seq:ggc)
     gene            complement(2056..>3101)
                     /locus_tag="VFJ67_03400"
     CDS             complement(2056..>3101)
                     /locus_tag="VFJ67_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7289460.1"
                     /translation="SYNQDGYGMSHMEKNNPDNHIPETTELGEVFKEHLNIRSISISG
                     LFLLAVFYTLYLARAVFIPIFFAIILKLLLAPAVDTLKRLRIPQALGSAVVIIALLAV
                     ISVSFYIFYQPAADWIERTPQSLRKVEQKLRFIKGPVEKVSEATKSMEKITDVGPKSE
                     DEEARLLESSPPALKAILLCALHTGMRKNEILSLKWDNVDIDKNLIILEHTNTKSKKT
                     RRIPIKSTLRKILLEQKLKFGSSNYVFLSTKGSPYKRHDSLKQSFAGACKRANISGLR
                     FHDLRHTAATRMIESGASIVAVSRILGHADLKTTMRYAHPEDTLKDAVETLTAYNPES
                     FTDKSTDLANMES"
BASE COUNT          669 a          787 c          715 g          930 t
ORIGIN      
        1 ctgagcgggt ttttgagctc gtgagcgagc ccgagcacta ggttctcgaa cttggcagcg
       61 gatatctcct ggatgctctt gctgctgaga aaattcgttc ccgacttgtc cttgatctgg
      121 agtatcgctc ccttgaggtc cccctcctgg gtgaaaatag gcgaagcaac cgcctggatc
      181 gaaatccgct ccccgctcct caatgtgggt tttatctcat cacctattac ggtccttccc
      241 tcccccatag ccataagcgc gacgcggtct atctcttcgg gcatgaacac tttcgaacgc
      301 tgtcccctgg ctttctccct cgatatcctg aaggtgcttt cggcattgtc gttaatgtcg
      361 agcacgcgga gctctttgtc tattatgatg atcgattccg gcaacgaatc gaatatttcc
      421 tcgaaattaa ttttctttgt catgggattc agcccgatta attccgaatg aacggcaaga
      481 gtcggtcacc cctgttcccg gatgcggagt atcgagattc gcgagactgc cgaccccttc
      541 cctatgtcat aggtcctcta ttcttataaa tgtcgttttc gcccgtattg tcgggaacct
      601 gtctatctcg cggagtgagg aaaggaggtc cttttccagg gactcatggg tcatgattac
      661 aaccgggacg tccccgcccc ccaggtgtct tcccttctgg ataaccgatt ctatgctgat
      721 accgtgttta ccgaggctcc cggtaatcct gccgagcacg cctggcctgt cttcggcctg
      781 gaaccttaaa tagtatttat tcgtcgtatc gctcatttta atcagcgggg acgacccctt
      841 tcccccgtag aatctgggag gggacgagtg tgtgttaccg agggacaggt ccttcgctat
      901 ctgcagcacg tcgctcacaa cggcgctagc cgtgggcatc attcctgccc ccttgccgta
      961 gagcatcgcc gggccgaccg cgtcgccgac gagataaata gcgttgaacg cgcccgatac
     1021 gtcggcgagc tgcgtttcct tcctcacgag cgccggatag accccggctt cgagtccttt
     1081 atccttcgac ttcgcaacgg caaggagctt tattttgtac ccgagctcgt cggcatatgc
     1141 gatatcgaga ggggttattc ctcttatccc ctcaacgtgg atctttccga aatctatgaa
     1201 tatgccgtag gacagcataa tgaggatcga gagcttgtga gcggcgtcta ttccatcaac
     1261 gtcgaatgag gggtcggcct cggcataacc ttctttctgg gcttctttca ggacatctga
     1321 gaactccgag ccctcctccg tcatcctgga tagtatgtaa ttggaagtcc cgttaattat
     1381 tccgtgtatg gaaagtatgt tgtttgcggc gaaactctcc tgtgtcacct tgataatggg
     1441 gattccccca cctacgcttg cctcgaaccc aatgtccact cccttctcca cggcggcgct
     1501 gaatatctcc ctcccgtagt gagcgaggag tgccttattg gccgtgacca cgtgcttccc
     1561 gttcctgatc gcgctcatca cgaaatccct tgcgatgccc gttccgccca cgagctcgat
     1621 gattatatgg atcgagccgt cctcgatcag ctcccttgcg tccctgatca gctttccttt
     1681 cgggattttt accggcctct tcctgtcggg gtctatgtcg gctatttttt tgagattcag
     1741 ccttatgcct gtcctctttt cgattatgtc cccgttctta ttgaggacct tgataactcc
     1801 gcatccgact gtgccggcgc cgatcaggcc gacgttcact acgctcatca aaattctcca
     1861 ttcaaatcga ggcaaaagtg agtataagct aggaattata cattatttta tttgacttta
     1921 aacgaatggc gacttaaatt aataccttcc gtggggccgt agctcagttg ggagagcgca
     1981 tgaatggcat tcatgaggtc gagggttcaa atcccttcgg ctccaccaat gttttcagag
     2041 agttacatta cccgttcaac tttccatgtt agctaagtca gtggatttgt cagtaaatga
     2101 ttctggattg tatgctgtaa gtgtctcaac agcgtctttt agagtatctt ctggatgtgc
     2161 gtatcgcatt gtagttttta ggtcggcatg acctaaaatt ctagacactg ctactattga
     2221 tgctcctgat tcaatcattc ttgtagctgc agtgtgtctt aggtcatgaa acctaagacc
     2281 tgaaatatta gctctcttgc atgctcctgc aaatgattgc tttaatgagt cgtgcctttt
     2341 gtatggagaa ccttttgtac ttaagaaaac ataattcgat gatccaaatt tcagtttctg
     2401 ttctaagagt atttttctaa gcgttgattt tataggaatt ctccttgtct ttttactttt
     2461 tgtgtttgtg tgttcgagga tgattagatt cttgtctatg tccacattat cccatttaag
     2521 acttagtatc tcattttttc tcattcctgt atgaagtgca caaagcaaaa ttgctttaag
     2581 ggcaggggga cttgattcta atagcctggc ttcctcatct tcggattttg gccccacgtc
     2641 tgtaatcttt tccatgctct tggtggcttc acttactttt tcgaccggcc ctttaatgaa
     2701 acgaagcttc tgttcaactt tcctgaggct ttggggagtc ctttctatcc agtctgcagc
     2761 aggttgataa aaaatataga atgagaccga tataacagcc aggagcgcta ttataactac
     2821 cgcagatccc aacgcctggg gtatgcgtaa tctttttagc gtatcgacgg caggagctag
     2881 tagaagtttt aagatgatag cgaagaaaat ggggataaat acggcacgcg caaggtagag
     2941 cgtgtaaaac accgcaagta ggaatagtcc tgaaatcgag atggaacgga tatttaagtg
     3001 ctctttaaac acctctccta actcggttgt ctcgggaata tgattgtcgg ggttattctt
     3061 ctccatatga ctcatgccat atccgtcttg attataactg a
//
LOCUS       DASSJH010000157         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_34236, whole genome shotgun sequence.
ACCESSION   DASSJH010000157 DASSJH010000000
VERSION     DASSJH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34236"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFJ67_03405"
     CDS             <1..1149
                     /locus_tag="VFJ67_03405"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET7289461.1"
                     /translation="PGEIDKVFIGNVIPPVRTPNIAREIALGSGVPPSVPAFTVTSAC
                     ASASHAFTSAVDSILSGDSGVVIAGGVDSAHDSSYLAGKGPKSRLYGSARGINLIDRA
                     MPLFKIGSGEDAISLPLLPERSSGHTLGEAAEKLARKYHISREDQDSFAVRSHTLASR
                     AQAEGIFEDEIIHTFVPPDFTDIVSADNCVRYDISSEALSALPPIFDKEFGTVTEGNS
                     APLSDGASLMLLMSEDKARALGYKPIAFVKSYAYAAVDPSDELLMAPAYSTPAALDRA
                     GIGLDDIDLIEVHEAFSSQVIAYVRALDSKGFAKEKLGRSQPVGEVDMERLNISGGSI
                     AIGHTLGATGTRIIMTLAYRMSKRKGNFGLVSLASAGGLGVTVVLEKE"
     gene            1155..>2209
                     /locus_tag="VFJ67_03410"
     CDS             1155..>2209
                     /locus_tag="VFJ67_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7289462.1"
                     /translation="MSSITYAIDNNGIAVLTVNTPGGSVNRLDQASFLYLEDFLNKID
                     RDLDMLESDDEIRAVVIASGKDGSFISGAQPREFLNFTLADEGRSYSLKAQELSEKME
                     NSRVPFVAAINGECLGLGLEIAIACKYRVAANTRKTVLGLDQIEFGLIPCAGGMLRLA
                     KLIGTKETLDMILAGETLDPHRAKQIGILDEVVPEEYLLSIAKKRALDMSDKEFKPRR
                     HRFGGMANALIKENPVSRKMYFDKAKKEIKESRDLYLTASRMALEALEIGVASFNRGL
                     HVESVYFGELAVTGYSRQLIRSSIAIDDVRNDARFAKAGGKTRKKTEKVAIIGEEDRG
                     AAIACLAAENGVSVRIK"
BASE COUNT          526 a          582 c          680 g          421 t
ORIGIN      
        1 cccggggaaa tagacaaggt atttatcgga aacgttatcc cgcccgtcag gacccccaat
       61 atcgcaaggg agatagcgct cggctcgggc gtcccgccct cggtcccggc ttttaccgtt
      121 acgagcgcgt gcgcgtctgc gagccatgct ttcacgagcg ccgtcgacag cattctttcc
      181 ggggactccg gtgtcgtcat tgcgggcggc gtcgattctg cgcacgactc gtcctacctg
      241 gccggcaagg gacccaagtc gaggctctac ggctcggcaa ggggcattaa tttgattgac
      301 agggcgatgc ctctcttcaa gataggctcg ggtgaggacg cgatctcgct cccgctcctg
      361 ccggagcggt cctcagggca tactctcggg gaagcggccg agaagctcgc gcggaaatac
      421 catataagca gggaagacca ggactccttt gccgtgagga gccataccct cgcttcgagg
      481 gcgcaggcag aggggatatt cgaggatgaa attatccata cgttcgtacc gcccgatttt
      541 acagatatag tctccgccga taactgcgtc agatacgata taagctcaga agcgctatcc
      601 gcactgcccc cgatattcga caaggaattc gggactgtga ctgaaggcaa ctccgcgcct
      661 ctgtcggacg gggcctcttt aatgctattg atgtcggaag acaaggcccg ggctctcggg
      721 tataaaccca tagcgttcgt gaaatcatac gcatacgccg cggtcgatcc ttcggatgag
      781 ctcctcatgg ctccggcata ttccaccccg gccgccctcg accgcgcggg gatcggtctc
      841 gacgacatcg acctcatcga ggtgcacgag gcgttctctt cgcaggtcat agcttatgtg
      901 agggcgctcg attcgaaggg attcgcgaaa gagaagctcg gaaggagcca gcccgtaggc
      961 gaggtcgata tggaaaggtt aaacatctcg ggcgggtcta tagcgatagg acacaccctc
     1021 ggcgccacgg ggacgaggat aataatgacg cttgcctaca ggatgtcgaa aaggaagggt
     1081 aatttcgggc tcgtgagcct cgcctcggcc ggcggactcg gggtcactgt ggtgctggaa
     1141 aaggaataat tctgatgagc agcataactt atgcgataga taacaacgga attgcggtgt
     1201 tgaccgtcaa cacgccgggc ggctccgtaa acaggctcga ccaggcgagc ttcctctatc
     1261 tcgaggattt cctcaacaag atagacaggg acctcgacat gcttgagagc gacgacgaga
     1321 taagggccgt ggtcatagcg agcggaaagg acgggagttt catcagcggg gcacagccta
     1381 gggagttcct caatttcact ctcgccgacg agggcaggtc ctacagcctc aaggcgcagg
     1441 agctctcgga gaagatggag aactcgaggg tccctttcgt ggcggctatt aacggagagt
     1501 gtctcggtct cggtctcgag atcgctatcg cctgcaaata cagggtcgcc gcgaatacga
     1561 ggaagacggt cctcggcctc gaccagatag agttcggcct aatcccgtgc gcaggaggga
     1621 tgctgaggct cgcgaagctt ataggcacta aagaaaccct cgacatgata ctcgcgggag
     1681 aaacgctcga cccccaccgc gcgaagcaga tagggatact cgacgaagtc gttcccgagg
     1741 aatatctcct gagcattgcc aagaaaaggg cgcttgatat gtcggacaag gaattcaagc
     1801 ccaggcggca ccgtttcggc ggcatggcga acgcgttgat aaaggaaaat cccgtatcca
     1861 gaaaaatgta tttcgacaaa gcgaaaaaag agataaagga gagcagggat ctctatctta
     1921 cggcttcgag aatggcgctt gaggcgctcg agataggggt cgcgagcttc aacagggggc
     1981 tccacgtgga atcggtatat ttcggcgagc tcgccgttac tggttattcg agacagctga
     2041 taagatcgag cattgccata gacgatgtaa ggaacgacgc gaggtttgcc aaggccggcg
     2101 gtaagaccag aaaaaaaacg gagaaggtcg cgatcatcgg cgaggaggac cgcggggccg
     2161 cgatagcctg tcttgcggct gagaacgggg ttagcgtgag gatcaaaag
//
LOCUS       DASSJH010000158         6592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1547405, whole genome shotgun sequence.
ACCESSION   DASSJH010000158 DASSJH010000000
VERSION     DASSJH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547405"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFJ67_03415"
     CDS             complement(<1..1080)
                     /locus_tag="VFJ67_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7289463.1"
                     /translation="MNFLAALKVSSRAIKSNKVRSILTTLGIIIGVAAVISLVSLTQG
                     ANRMIEEQLTSLGGKSLFINPGKRGVGIQDNAIDLTAEDADAIRKLDIVKHVSPMIDA
                     PEQVVWGNRNWFTVVVGTSPDFVFINDWYPSEGVFFNNEDTARTENVCVLGTTVVENL
                     FGYIDPVGQTVRIGKNSFRVIGVMTALGQTTGGRDQDDVALIPYTTFQKRILGSSALE
                     SISVSVESPGDIPAAETMIRSVLSERHKLGVNEEDSFYIKSQMGVIDRIFAISNIMTI
                     LLGSIASISLVVGGIGIMNIMLVSVGERTKEIGIRMAVGARERDIMTQFLIEAVLLSL
                     TGGVIGIAVGVLGSKIASSFTGWKTV"
     gene            complement(1077..1802)
                     /locus_tag="VFJ67_03420"
     CDS             complement(1077..1802)
                     /locus_tag="VFJ67_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7289464.1"
                     /translation="MNGNVIVTKDIHKVYKLGDTEVHALRGVSVDIREGEFVSIMGAS
                     GSGKSTFMNIIGCLDKPTSGTYYLDGNRVSDMGKDELAGIRNVKIGFVFQSFNLLART
                     TAVENVELPMIYGSIPARERTKLAGEALSRVGLRGREKHLPSQLSGGQQQRVAIARAI
                     VNDPNMILADEPTGNLDTSTSLEIMEIFKSLNEQGKTVIIITHENDIAECAERIIVFR
                     DGSIIEQKRRNGGAHAAKETINT"
     gene            complement(1806..2954)
                     /locus_tag="VFJ67_03425"
     CDS             complement(1806..2954)
                     /locus_tag="VFJ67_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7289465.1"
                     /translation="MNLINKKTIAGLIVVILLAVFLVPRLFGGKDNRITYRTAEADRN
                     DVISLISATGTLNPLNSVAVGSLVQGNVDKIYADFNTEVKTGDALAEINPGPLTIELK
                     KAEAEKKKAEADFTIANSLYKANKELYEKRLIPKEEFDDSQAKYSSALAAFDQTKVAL
                     EIASTNLENTVIRSPIDGVVLSRNVNAGESVTPGGKALYVIASDLGEMKIDTKVSEAD
                     IGKLVPDQKAYFKVDAYPGETFPGTVSQVRNEPIINNNVVTYNVIVLTENKEGKLKPG
                     MTAEVKIVVADKKDVLRVPTAALRFIPPSSADIKERPDELSGNSYVWIKLTNGQIGAV
                     PVKTGVSDDAYTEILDGDLKEGQKVIVEAVSGSKADTGSSYLPQPRRF"
     gene            complement(2938..4179)
                     /locus_tag="VFJ67_03430"
     CDS             complement(2938..4179)
                     /locus_tag="VFJ67_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7289466.1"
                     /translation="MKSAGTLFILFFMLLLSLSTAGAQEKKVLSLDEAVSIALKENPG
                     ITVSEANIEISKALVRNMQAPYYPDVYTRIIVPFVGRESGFFLDQMIWDFGQTSNKVK
                     SSKEQLKSTKFDSQAAKEDIELNTIVSYYNVLSQTHLVESYEKKVTEDEKRFERAEGF
                     YKAGRVSQTDVTKEEVNLGNARMQLAAARNDLEIAEDNLMAAMGVEGEFNFELVDTLE
                     YDKKDIDLDDSIDKALDTRPELKSLKAQEASMKANLTASKRAFYPQFFGRTAYRFKGE
                     GAETPGFIAGVGLQFPIFDGFARSANVQDAKAKLMKSQAQIETAKVEIAAEVKQLKLD
                     LELAEENIQITEKTKESAEKSFLLAQERYRLERASELELAEAESLYSSSEAAYRQSIY
                     NYNITAARLERAIGGTDESDK"
     gene            4279..5436
                     /gene="hemW"
                     /locus_tag="VFJ67_03435"
     CDS             4279..5436
                     /gene="hemW"
                     /locus_tag="VFJ67_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET7289467.1"
                     /translation="MAFGVYIHIPYCVKKCPYCDFNSWGIKGGFPESDYTESVLRELE
                     LYRDEIEGRELTSVFFGGGTPSLFDPGSIESIISKIYGLTSHAPDIEVSLEVNPKTAD
                     LEKLRGLREAGVNRISIGVQSFSQRKLEFLGRINSPQDGRRILDEVRSAGFLNYNFDL
                     MYGTGDETPEEWRSDLEEAVTFGSTHISAYCLTIEKGTEFGALFSKGKLLLPGEESLS
                     EFIDFTTAFLAGAGYSQYEISNYARPGFECRHNLLYWKGEDYIGLGAGAHSHLKSAPG
                     SPWGHRWANVRPPVSYMKAVAEGKRPVDFSERLTMEEAVEDRIIMGFRLNEGLDVKGL
                     RNNYGVSPDRGRMESLSEDGFLEMSGDVIKLTKKGTLLSDEIIVRLVSSLG"
     gene            complement(5442..5897)
                     /locus_tag="VFJ67_03440"
     CDS             complement(5442..5897)
                     /locus_tag="VFJ67_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289468.1"
                     /translation="MAKEPKKGETRYRFFIDKSGNWFQDGIKIRHRGTYLYNNRLLDV
                     DREGQFFIDEGSGRLYAEVEDAPFVVKMVHRRGGEFYLRLNDETEEALDFGSLRMSGE
                     NVPYAMVKNGRFEARFLRPAYYELMKYADKDGSDYFIETGGVKHYIKRT"
     gene            complement(5944..6357)
                     /locus_tag="VFJ67_03445"
     CDS             complement(5944..6357)
                     /locus_tag="VFJ67_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7289469.1"
                     /translation="MKTLTEYLWFNTNEKREMVHITNTIRDIVHKSGVKDGFALVSAM
                     HITAAVYVNDLEDGLIEDIWEWLEHLAPYRRDYRHHRTGEDNGDAHLKNLIMHHQVII
                     PVTKGDLDLGPWQRVFYAEFDGGRRKRVIVKVMGE"
BASE COUNT         1268 a         1968 c         1686 g         1670 t
ORIGIN      
        1 tactgtcttc catccggtga acgaagaggc gatcttcgac cccagcaccc caaccgctat
       61 ccctataacc ccccccgtga gcgagaggag cacggcctct atgaggaact gggtcattat
      121 gtctctctcc ctcgccccga cagccatcct tataccgatc tccttggtcc tttcccccac
      181 ggatacgagc attatgttca tgattcctat gccgcccacg acgagagaga tcgaggctat
      241 gctgccgagc aggatggtca tgatgttcga gatggcgaat atcctgtcta tcacccccat
      301 ctgggatttt atatagaaac tgtcctcttc gttcacgccg agcttgtgcc tttcgctaag
      361 gacggatctt atcatggttt cagcggccgg gatgtcgccc ggcgattcca cggacaccga
      421 tatcgattcg agcgcgcttg agccgagtat cctcttctgg aatgtcgtgt acgggatgag
      481 cgccacgtcg tcctggtccc tccccccggt cgtctgcccg agcgcggtca tgacccctat
      541 caccctgaac gaattcttgc cgatcctgac cgtctgcccc acggggtcaa tgtatccgaa
      601 gaggttctcg accaccgtcg tgccgagcac gcagacgttc tctgtccttg ccgtgtcttc
      661 gttattgaaa aatacccctt ccgacgggta ccagtcatta atgaagacga aatcggggct
      721 cgtgcccacg acaacggtga accagttcct gtttccccag accacctgct cgggcgcgtc
      781 tatcatcggc gagacgtgct tcactatgtc gagcttcctt atggcgtccg cgtcctcggc
      841 cgtgaggtct atcgcattgt cctggatgcc gacgcccctt ttgccgggat ttatgaaaag
      901 cgacttcccg ccgaggctcg tgagctgctc ctcgatcatc ctgttggcgc cttgagtgag
      961 ggagacgagt gaaatgacgg ccgcgacgcc gatgatgatg cccagggtcg taagaatcga
     1021 ccttaccttg ttcgacttta tggcccttga agagaccttc aaagcggcta ggaaattcat
     1081 gtgtttattg tttccttagc tgcgtgagcg ccgccgtttc ttctcttttg ttcgattatt
     1141 gagccgtccc tgaatacgat tatacgctct gcgcactcgg ctatgtcgtt ctcgtgagtg
     1201 attatgatta cggtcttgcc ctgctcgtta agggatttga atatctccat gatctcgagg
     1261 ctcgtgctcg tgtcgaggtt ccccgtgggc tcgtcggcta gtatcatgtt cgggtcgttc
     1321 acaatggcgc gcgcaatggc gacccgctgc tgctgccccc ctgagagctg agacgggaga
     1381 tgtttctccc ttccccggag ccccactcgc gagagcgctt cccctgcgag cttcgtcctc
     1441 tcccgtgcgg gaatgcttcc gtatatcatg ggcagctcca cgttctcgac tgcggtcgtc
     1501 ctcgccagca ggttgaagct ctggaatacg aaccctatct tgacgttcct tatccctgcc
     1561 aactcgtcct tgcccatgtc ggagacccta ttgccgtcga gataatatgt cccgctagtc
     1621 ggcttgtcga ggcagccgat tatgttcatg aacgtgcttt tacccgaacc cgaggccccc
     1681 attatcgaca cgaattcccc ctccctgata tcgaccgaga cccccctcag cgcgtgcacc
     1741 tccgtgtcgc cgagcttata caccttgtgt atgtccttcg tgacgatgac gttgccgttc
     1801 atccgtcaga atctcctcgg ctgagggaga tacgacgatc cggtgtcggc tttggagccg
     1861 gatacggcct ccacgatcac cttttgtcct tccttcaaat ccccgtccag tatctctgtg
     1921 taggcgtcgt cgctgacccc ggtcttgacc ggcacggccc ctatctgtcc gttcgtgagc
     1981 ttgatccaga cgtacgaatt gccggagagc tcgtcgggcc tctcttttat atcggccgag
     2041 gagggcggta tgaacctgag cgcagccgtc ggtaccctga gcacgtcctt tttgtcggcg
     2101 acgactatct tcacctcagc agtcataccg ggcttaagct tcccctcttt attctccgtg
     2161 agtactatca cgttatacgt gaccacgttg ttgtttatta tcggctcgtt cctcacctga
     2221 gagacggtcc cggggaaagt ttcgccggga tatgcgtcca ctttgaaata ggccttctgg
     2281 tcggggacga gctttccgat gtcggcctcg ctaaccttgg tgtcgatctt catctccccg
     2341 aggtcgctcg ctatcacgta aagcgccttc cctccgggcg taacggattc tcccgcgttc
     2401 acgttcctcg aaagcacgac gccgtcgata ggggacctta taacagtatt ctccagattg
     2461 gtcgaggcga tctcgagggc gaccttcgtc tgatcgaacg cggcgagcgc cgaggaatat
     2521 tttgcctgcg agtcgtcgaa ctcctctttt ggtataagcc ttttttcgta gagctccttg
     2581 ttcgccttgt aaagagagtt cgctatcgtg aaatcggcct ccgccttctt cttttcggcc
     2641 tccgccttct tgagctcgat tgtgaggggc cccggattta tctccgcgag cgcgtctccc
     2701 gtcttcactt ccgtattgaa gtcggcgtag attttgtcga catttccctg cacgagagag
     2761 cccaccgcca ccgagtttag agggttgagc gtccctgtcg ccgaaatgag cgatataaca
     2821 tcgttcctgt cggcttcggc cgtcctgtac gtgatacggt tatccttccc gccgaagagc
     2881 ctcggcacaa ggaagacagc cagcaatata acgactatca gccccgcgat ggtcttctta
     2941 tttatcagat tcatcagttc cgcctatggc gcgctcgagc ctagcggccg ttatattata
     3001 gttgtaaata gactgcctgt acgccgcctc gctcgatgag tatagggact ccgcctcggc
     3061 gagctcgagc tcggacgccc tttcgagcct gtatctttcc tgcgcgagga ggaagctctt
     3121 ctccgccgat tccttcgtct tctccgtaat ttgaatgttc tcctccgcga gctcgaggtc
     3181 gagcttgagc tgctttacct cggccgctat ctcgaccttt gcggtctcga tctgcgcctg
     3241 ggatttcatc agcttggcct tggcgtcctg gacgttggcc gacctcgcga acccgtcgaa
     3301 tatcgggaac tgaagtccca cacccgctat gaagcccggc gtctccgccc cctctccctt
     3361 gaacctgtat gcggtccttc cgaaaaactg cggataaaac gctcttttcg aagccgtgag
     3421 gttagccttc atcgaggcct cctgcgcttt gaggctcttc agctccggtc tcgtatcgag
     3481 cgctttgtct atggaatcgt cgaggtcaat gtccttcttg tcgtattcga gcgtatcgac
     3541 gagctcgaag ttgaattcgc cctcgacgcc catggccgcc atcaggttat cctctgcgat
     3601 ctcgaggtcg ttcctcgccg ccgccagctg catcctggcg tttccgagat tgacctcttc
     3661 cttcgtcacg tctgtctgcg agaccctgcc cgccttgtag aacccctcgg cgcgctcgaa
     3721 cctcttctcg tcctccgtga ccttcttctc gtaagactcc acgagatgcg tctgtgagag
     3781 gacgttgtaa tagctgacga tggtgttcag ctcgatgtcc tccttcgccg cctggctgtc
     3841 gaacttcgtg gacttgagct gctccttgct cgacttcact ttattggacg tctgcccgaa
     3901 atcccatatc atctggtcga ggaagaaccc cgattcgcgg cctacgaacg gcacgatgat
     3961 ccttgtatat acatcggggt aataaggggc ctgcatgttc ctcacgagcg cctttgagat
     4021 ttcgatattc gcttccgaaa ccgtgatgcc ggggttttcc ttgagcgcta tgctcacggc
     4081 ctcatcgagc gaaaggacct tcttctcctg ggcccctgcc gtgctcaggc tgaggagcaa
     4141 catgaaaaac aggatgaaaa gtgtacccgc gctcttcata tcatatctct attaaagcaa
     4201 taatatgacc ctaaaacata tttgttgcaa tatcccccgt ctgttgcatc accccctgag
     4261 ccgccgtaaa atacctgaat ggccttcggc gtatatatac atatacccta ctgcgtgaaa
     4321 aaatgcccct actgcgattt caactcctgg ggcataaaag ggggcttccc cgaatccgat
     4381 tacactgaat ccgtactcag ggagctcgag ctttacaggg acgagataga gggacgcgag
     4441 ctcacctcgg tctttttcgg cggcgggacg ccttctctat tcgaccccgg cagcatagaa
     4501 agtataatct cgaagatcta cggactaaca tcccacgcgc cggacatcga ggtctcgctc
     4561 gaggtaaatc cgaaaacggc cgatctcgag aagctcaggg gtctccgcga ggcgggtgtg
     4621 aacaggataa gcatcggtgt ccagtccttt tcccaaagga agctcgaatt cctgggaagg
     4681 ataaacagcc ctcaagatgg ccgtaggata ctcgacgaag taaggtctgc gggattcctg
     4741 aactataact tcgacctcat gtacggtacg ggcgatgaaa cccctgaaga atggcgtagc
     4801 gacctcgaag aggccgttac tttcggctcg acccacattt ccgcctactg cctgactata
     4861 gagaagggaa ccgagttcgg cgcgctcttc agcaaaggaa agctcctcct tcccggggag
     4921 gaatctcttt cggagttcat cgatttcacg acggccttcc tcgccggagc tggctactcg
     4981 cagtacgaga tttccaacta cgcgaggccg ggcttcgagt gcaggcacaa tctcctatac
     5041 tggaagggag aggattacat cggtctcggc gcgggggcgc attcgcatct caagagcgcc
     5101 ccgggctccc catggggaca caggtgggcg aacgtcaggc cccctgtctc ttacatgaag
     5161 gctgttgcgg aaggaaagag gcccgtcgat ttttccgagc gtctcactat ggaagaggcc
     5221 gtagaggacc ggatcatcat gggtttcagg ctgaacgagg gtctcgacgt gaaggggctt
     5281 cggaataact acggcgtctc tcccgacaga gggcgtatgg agtccctcag tgaagacggc
     5341 tttctcgaga tgtcgggcga tgtgataaag cttacgaaga agggcacgct cctctctgac
     5401 gagattatag tcaggctcgt gagctcactc ggctaacagg gtcaggtcct tttgatgtag
     5461 tgtttaaccc ccccggtctc tataaaataa tcgcttccgt ctttgtccgc gtatttcatc
     5521 agctcgtagt atgccggcct caagaacctc gcctcgaacc tgccgttctt taccatcgcg
     5581 taaggcacgt tctcaccgct catcctgaga gagccgaaat cgagcgcttc ctcggtctcg
     5641 tcgttcagcc tgaggtagaa ttccccgccc ctcctgtgga ccatcttcac gacgaaaggc
     5701 gcatcctcaa cctccgcgta aagcctgccg ctcccctcat cgatgaaaaa ctgaccttcc
     5761 ctgtccacat cgaggagcct gttgttatag aggtacgttc cccggtgtct tatttttatt
     5821 ccgtcctgaa accagttccc ggacttgtct atgaagaacc tgtatctggt ctcgcctttt
     5881 ttgggctctt tcgccatggg tatctagtat aacggagtgt gctcgaggaa acctttaagt
     5941 ccttcactcc cccatgacct tcacgatgac cctctttctt ctgccgccgt cgaactccgc
     6001 gtagaacacc ctctgccagg ggcctaagtc gagatcccct ttcgtcacgg gaataatgac
     6061 ctggtggtgc atgatgaggt tctttaaatg ggcgtccccg ttgtcctcgc ccgtcctgtg
     6121 gtgcctgtaa tcacggcggt agggggccag gtgctcgagc cactcccata tgtcctctat
     6181 gagcccgtcc tcgagatcgt tcacgtaaac tgcggccgtt atgtgcatcg cggacacgag
     6241 ggcgaacccg tccttgacgc cgctcttatg cactatgtcc cttatcgtat tcgttatatg
     6301 caccatctcc ctcttctcat tcgtgttgaa ccacaggtac tcggtcaggg ttttcataat
     6361 gtcctcccgc gcatgttgta ttatatgagt gtaattccat taagggggag atacgagcat
     6421 gataatagag ctgagcgtaa tccctatagg cgcgggaacg tctctctcgg agtacgtcgc
     6481 cgaggtaatg agggtcatcg agtcgagcgg tctcaaatac gagtcccatt ccatgggtac
     6541 gaacatcgag tgcggatggg acgacatcac tccactagtc agaaagtgcc ac
//
LOCUS       DASSJH010000159         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_897050, whole genome shotgun sequence.
ACCESSION   DASSJH010000159 DASSJH010000000
VERSION     DASSJH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897050"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /gene="dnaE"
                     /locus_tag="VFJ67_03450"
     CDS             complement(<1..1988)
                     /gene="dnaE"
                     /locus_tag="VFJ67_03450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET7289470.1"
                     /translation="MSNGFVHLHLHSQYSLLDGAIKFEDLFPLAKEFGMEAVALTDHG
                     NLFGAYEFYKKAKESGIKPIIGCELYVTQKINPDNPSEGKTHHLTVLSMNGAGYRNLS
                     RLVTKAYFDGFYRRPRVDHELLSTHNEGLIVLSGCLNSELAKAIFNKDMKAALNVAAM
                     YREIFGDRYYLEVQATGLPEQKRVNKKVREIGEKLGIPIVATNDCHFLRREDSRPHDA
                     LLCIQTGSSLQDENRFRFQGDQYYLKAEGEMMKDLEGFEDAIRMTSKIAERCNFDFER
                     DGYKLPVFEAEGGESLDEYLARLAREKLEGRLTENSIPDEKRGEYRSRLESEIKIIQK
                     MGFSGYFLVVADFINYAKSSGIPVGPGRGSAAGSLAAYALGITEIDPIPYNLIFERFL
                     NPERVSMPDIDIDFCGEGRDEVIRYVTEKYGADKVAQIGTFGTMSAKAVVKDVGRVMG
                     IPYADVDRVTKLIPSFRGKVFSIDKAIESVAELKKLIDDSPALKEMIELAKPLEDMVR
                     HSSTHAAGIVIANEPLADHIPLCKGSRNEIVTQFDMNSIEELGFVKFDFLGLKTLTVI
                     NKALELIKENYGNGEEFKLDINKIPLDDPEVYALLSSGRTRGIFQIESSGMKELMINL
                     EPSGFEDIIAVLALYRPGPLDSGMVDEYIERKHGKRKI"
     gene            2266..>2590
                     /locus_tag="VFJ67_03455"
     CDS             2266..>2590
                     /locus_tag="VFJ67_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289471.1"
                     /translation="MEEILSMLNTIPSPYSDLVAVSVIFLILLLISLLIAFLFEAKPG
                     KEAVKPEAEKPAEIEAEKHLPAPDLEVFKAPPAGERPAEVFRAPVEREEAPVKAPEKA
                     APEAVE"
BASE COUNT          521 a          711 c          680 g          678 t
ORIGIN      
        1 tgaatcttcc tcttcccgtg cttgcgctct atgtactcgt ctaccatgcc gctgtcgagc
       61 gggcccggcc tgtaaagcgc gagtacagct attatgtcct cgaaccccga gggctcgaga
      121 ttgatcatga gctctttcat gccggatgat tcaatctgga atatgccgcg tgtccttccg
      181 ctcgaaagaa gcgcgtagac ctccgggtcg tcgaggggta ttttattgat gtccagcttg
      241 aattcttctc cgttcccgta gttctccttg ataagctcga gcgccttgtt tatcaccgtg
      301 agcgtcttaa ggccgaggaa atcgaacttg acaaaaccga gctcttcgat cgagttcatg
      361 tcgaactggg tgacgatttc attcctcgag cctttgcaga gaggtatatg gtcggcgagc
      421 ggctcgttcg ctatcactat gcccgccgca tgggtggacg aatgcctgac catgtcctcg
      481 agcggctttg cgagctctat catctcctta agcgccgggg aatcgtcgat cagcttcttt
      541 aattcggcca cgctctcaat cgccttgtct atgctgaata ccttccctct gaaagacggt
      601 atcagctttg taaccctgtc gacgtcggcg tacggtatgc ccatgaccct gcctacgtcc
      661 ttcaccacag ctttcgcgga catggttccg aacgtgccta tctgggcgac tttatcggcc
      721 ccgtattttt ccgttacgta tctgattacc tcgtccctgc cctccccgca gaagtcgatg
      781 tctatatcgg gcatgctgac gcgctcgggg ttgaggaatc tttcgaagat taggttataa
      841 gggatggggt cgatctcggt gatcccgaga gcgtatgcgg ccaggctccc ggccgcgctc
      901 cccctgcccg ggccgacggg aatgccgctt gatttggcgt aatttatgaa atccgcaacg
      961 acgaggaagt agcccgagaa gcccattttc tgaataatct tgatctcgct ttcgagcctg
     1021 ctcctgtact ccccgcgctt ttcatcgggt atggaatttt ctgttaacct gccttccagc
     1081 ttttctctcg cgagccgggc cagatactcg tcgagcgact cgccgccttc ggcttcgaat
     1141 accggtagct tgtatccgtc cctctcgaaa tcgaaattgc acctctcggc gatcttagac
     1201 gtcatcctta tcgcgtcctc gaatccctcg aggtccttca tcatctcgcc ttcggctttg
     1261 agataatact ggtcgccctg aaacctgaac cggttctcgt cctggagcga cgagccggtc
     1321 tgtatgcaga ggagcgcgtc gtgcggacgc gagtcctcgc gtctcaagaa atggcagtca
     1381 tttgtagcga ctatgggtat gccgagcttt tcgcctatct cccttacctt tttattgacc
     1441 ctcttctgct cgggcagacc cgtggcctgg acctccaggt aatacctgtc gccgaagatt
     1501 tccctgtaca tggcggctac gttcaatgcc gctttcatat ccttgttgaa aatcgccttg
     1561 gcgagctcgc tgttgaggca tccgctaagt actatgagac cttcgttgtg cgtgctgagg
     1621 agctcgtgat cgaccctcgg cctcctgtaa aacccgtcga agtaggcctt tgtcacgagt
     1681 ctcgagaggt tccggtatcc tgccccgttc atggagagca ccgtaaggtg gtgagttttg
     1741 ccctcggacg ggttgtcggg gttgatcttc tgcgttacgt agagttcgca ccctatgatg
     1801 ggtttgatgc ccgattcctt cgctttttta tagaattcgt acgctccgaa gaggttcccg
     1861 tgatcggtaa gggcgaccgc ctccatgccg aactctttgg cgagagggaa gaggtcctcg
     1921 aactttatcg cgccgtccag cagtgaatat tgggagtgca gatggaggtg gacaaaaccg
     1981 ttagacatca gtttttatta tatcagtgaa ggctcgggct gttcaagtgt atcgaaaaca
     2041 tcatttgatt cttacgggtt acagagagac aaattttatt gcctttggta ccgtacaaag
     2101 gcgtaacgga ctcatcgagg gctcgttgtt gaaaagaatt ccctgaaaag tatattaacg
     2161 gtaggcatat aaagacatcg gacggaaatc ttccgcacag cgcaatttat ccagtccaat
     2221 gtccgcccgt taataaggtg tgtcatcaag gaggcttttg gcattatgga agaaatttta
     2281 tcaatgttga atacgattcc gagcccttac tcggacctgg tcgccgtaag cgtaatattc
     2341 ctgatattac tcctcatctc cctcctcatc gcgttcctct tcgaagcaaa gccggggaag
     2401 gaggcagtca agcccgaagc ggaaaaaccg gccgagatag aggccgagaa gcatcttcct
     2461 gcgcccgacc tcgaggtgtt caaagcgcct cctgccggag aacggcctgc cgaggttttc
     2521 cgggccccgg tcgagcgcga ggaagcgccg gttaaagcgc ctgaaaaggc tgcgccggag
     2581 gcagtggaag
//
LOCUS       DASSJH010000160         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1006769, whole genome shotgun sequence.
ACCESSION   DASSJH010000160 DASSJH010000000
VERSION     DASSJH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006769"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFJ67_03460"
     CDS             complement(<1..422)
                     /locus_tag="VFJ67_03460"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET7289472.1"
                     /translation="MKWYFAPLLAVTLLAASIQTALPVNSATTAYTAKIATLLPKEAR
                     NNGKLGVVVKSLTSGETIYEHNAQNLFIPASNEKIITSVAALSLLKRDYRFKTEFYSG
                     GGISKGVLHGGLYVKGYGDPTFGEPHLGFIVYQLKKRG"
     gene            616..1335
                     /locus_tag="VFJ67_03465"
     CDS             616..1335
                     /locus_tag="VFJ67_03465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7289473.1"
                     /translation="MALASRNIKKTKKAFFFDFGDTLASTDPPFIFRIAMAMRKAGFD
                     ITDREFEAEYVKADYRLFLKHKEMGGITPRQHREWFFPLLYDSLAPVPDINRFRENVR
                     REMSGINFSRSLVAGASELLDFLKGKGYLLAVISNNDGYTEEKCEEVGIRRYLDYVFD
                     STRLDMVKPDPGIFKYAADRMGISPSEAVHVGDMYGTDVMGALHAGADVIWFNGRRMD
                     KFDDADVPEVSELSQIIGLVD"
     gene            1418..>2483
                     /locus_tag="VFJ67_03470"
     CDS             1418..>2483
                     /locus_tag="VFJ67_03470"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HET7289474.1"
                     /translation="MDGEIKIEGARENNLKNINVSIPWHKFTVVTGLSGSGKSSLALD
                     TLYAEGLRRYIECLSTYARQFLERVDRPDMDDISGLPPAVAIESRNTVRNSRSTVGTT
                     TEVYDYMRLLFAKIGRIFCPECGREVRHRSPGDIAEELLRGHGGERVLITFPPGNLNL
                     TPAELLSNGFTRIIKDGEVAEVEGLPKIPAGAEIVLDRLLLDKKSRPRLVGSLELAFE
                     ESRRVNVRIVDGDVLKFSADLECPEGHMRFNEPTPLLFSFNSPQGACPECKGFGNILG
                     LDPDLVVPNPDKSLSQGAIEPLTKPSLKPQMRKLLHFAEERGIYTDKPYASLPAKEKE
                     LIFEGGAGFPGVRGYFRRLEE"
BASE COUNT          605 a          621 c          708 g          549 t
ORIGIN      
        1 ataccccttt tcttaagctg atagacgatg aatcccaggt gcggctctcc gaatgtgggg
       61 tcaccgtaac cttttacata aagaccgccg tgcagaacgc ctttcgatat gccgcctccg
      121 ctgtagaatt cggtcttgaa cctgtaatca cgcttaagga gagagagcgc agccacggaa
      181 gttattattt tttcgttcga agccggtatg aagagatttt gcgcattgtg ctcgtagatg
      241 gtctcccccg aggttaggga cttgaccaca acgccgagct tgccgttgtt cctggcctct
      301 ttcggcagca gggtcgcgat ttttgctgtg taggctgttg tcgcgctgtt tactggcagt
      361 gcggtctgaa ttgaggcagc taagagggtg accgcaagca aaggagcaaa ataccatttc
      421 atattaggaa aaatattcta acatctccgc gttttcattg caaagaaaaa ttgagatatt
      481 atcggaatta atccgattat cggggtgttt agtacggtat ctccgaaaaa accgggcgcg
      541 gccgcactac agatgcagaa ttagtggaac ttaacacata tgctatcatt ttctctatag
      601 ggggcgcatg aagaattggc actagcttcg aggaatataa aaaagacaaa gaaggcgttt
      661 ttcttcgatt tcggcgacac gcttgcatcg acggatccgc cgtttatctt caggatcgcc
      721 atggcgatga ggaaagccgg tttcgatatt acggacaggg aattcgaagc ggaatacgta
      781 aaagccgatt acagactctt cctgaaacat aaggagatgg ggggcattac accgcggcag
      841 caccgggaat ggtttttccc gctcttatat gattcgttag cccctgtacc cgatataaac
      901 aggttccggg agaacgtgag aagggagatg agcggaatca atttctccag atccctcgtg
      961 gcgggggcct cggagcttct cgatttcctc aagggcaagg gttacctgct ggccgtgatc
     1021 tcgaataatg acggctacac ggaggaaaaa tgcgaagagg tcgggatcag gcggtatctc
     1081 gattacgtct tcgattcgac aaggctggat atggtcaaac ccgaccccgg tatcttcaaa
     1141 tacgccgctg acaggatggg tatatctccg tcggaagcgg tgcatgtggg ggacatgtac
     1201 ggcacggacg tcatgggcgc gctgcacgcg ggggcggatg tcatatggtt taacggaagg
     1261 agaatggata aattcgacga cgccgacgtg ccggaggtaa gcgagctctc ccagataatc
     1321 ggactcgtgg attaatcaga caatcggacc gcaaattcag gccgccccgc cgggacggat
     1381 cctgttcatc tcataattaa attataaagg caggctcatg gacggcgaaa tcaaaatcga
     1441 gggcgccagg gaaaacaact taaagaacat taatgtctcc ataccctggc ataaattcac
     1501 ggttgttacg gggctaagcg gatcgggaaa gtcctcgctg gccctggata cgctctacgc
     1561 ggaaggtcta aggaggtaca tcgagtgcct gtccacctat gcgagacaat tcctcgaacg
     1621 cgtggaccgg cctgatatgg acgatatatc gggactccct ccggctgtcg ccatagagag
     1681 caggaacaca gtcaggaact cgcgctcgac cgtcggcacc acgacggagg tctacgatta
     1741 catgcggctc ctcttcgcga agatcggcag aatcttctgc cccgaatgcg gcagggaggt
     1801 cagacacagg tctccggggg atatagccga ggagttgttg cgcggccatg ggggagaaag
     1861 ggtgctgatc acgttcccgc ccggcaattt aaatctgacg cccgccgagc tgttgtcgaa
     1921 cggctttacc aggatcataa aggacggcga agtagcggag gtcgaaggtc tcccgaagat
     1981 tcccgcgggc gctgagatcg ttctcgaccg gcttctactg gataagaaat cccgcccgag
     2041 gctcgtaggc tctctcgagc tcgcgttcga ggagagcagg cgtgtgaacg tccgtatagt
     2101 cgacggcgac gtcctgaaat tttcagccga tctcgagtgc cccgagggtc atatgcgttt
     2161 caacgagccg acccctctgc ttttttcctt caacagccca cagggagcat gcccggagtg
     2221 caagggcttc ggcaacatac taggtctcga tcccgacctc gtcgttccca atccggataa
     2281 gagcctgtcg cagggggcga tagagccgct aacgaagcct tctctcaagc cgcagatgag
     2341 gaagctcctc cacttcgcgg aggaacgggg tatctatacc gacaagcctt acgcgtccct
     2401 cccggctaag gaaaaggagc ttatcttcga agggggagcc ggctttcccg gcgtaagagg
     2461 atatttcagg cgtcttgagg aaa
//
LOCUS       DASSJH010000161         5783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1442718, whole genome shotgun sequence.
ACCESSION   DASSJH010000161 DASSJH010000000
VERSION     DASSJH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442718"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1667
                     /locus_tag="VFJ67_03475"
     CDS             300..1667
                     /locus_tag="VFJ67_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET7289475.1"
                     /translation="MTYINPQIFREYDIRGIVDKDLNDDVLERIGKAYGTYVKEAGAK
                     VVSIGRDCRLSSPAYAKAMTRGINSTGVDVIDIGMVSTPMLYFSLYNLEVDGGVMITA
                     SHNPGEYNGIKLCRGRDSVFGEQIQKIREIAEAGEFAAGKGSSIETDIEELYVGFLRE
                     NIQIKPGVRAAVDYGNGMVGSIGPRVFKEFGCDVTELYVTPDGTFPNHHPDPTVEENL
                     EKLIETVRSGRYEIGIGFDGDGDRIGVVDEKGNIVWGDMLVLVFARDVISRKPGAKII
                     GDVKCSNRLFNGITEAGGEAIMWKTGHSLIKDKMKTEKASLAGEMSGHIFFADKFFGY
                     DDALYASLRILEIISRTGKPLSRLLEGIPPAISTPEIRVDCPEAFKFKAVEMVKAELG
                     GKYRIIDIDGVRVEFPDGWGLIRASNTQPALVLRFEAQTEERLSEIRGIIEGELRAAM
                     KKLSA"
     gene            complement(1678..1911)
                     /locus_tag="VFJ67_03480"
     CDS             complement(1678..1911)
                     /locus_tag="VFJ67_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289476.1"
                     /translation="MMNFRAEYGEYSAQEAVDAILVLLRQNGVEIDEKSFTWTLVGAL
                     FEKGIITRKEFERIYFSLKNHEVNGNRSKENVL"
     gene            complement(2110..3249)
                     /locus_tag="VFJ67_03485"
     CDS             complement(2110..3249)
                     /locus_tag="VFJ67_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7289477.1"
                     /translation="MSKKILGGREKVKAAVVQASPVFMDKGRTVEKACALIREAGRNN
                     AELVAFSEAFIPCYPAYYTVGYETPPHEWTDYMIALQENSLVIPSEDTRILGEAARDA
                     GAYVVIGCNELDDRPGSQTVYNTLLFIDKNGEVLGRHRKTMPTYTERLYWGMGDASDL
                     RVFDTDIGRIGGLICWENHMTLIRAAMIHRGEEFHIAVWPGNWKRGEDHLLDADTSPG
                     GALCNLQSLIKVHAFEAGAFVLSGCGYLDSDDFPEKWHYIRDGGHINYDWARGGSSIV
                     NPAGRYLAEPNFEKDAILYAECHANQIKAVKAVFDSLGHYSRWDIAQLSLREGPWEPE
                     VPAGRPYAPDPDLPASELRRISEEYEISVESLEAILREVGRLGKA"
     gene            complement(3266..3658)
                     /locus_tag="VFJ67_03490"
     CDS             complement(3266..3658)
                     /locus_tag="VFJ67_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289478.1"
                     /translation="MTDQGQPLGGGQQSEFGNIGSSILSAFNLGVSYNQQTGGLKSVT
                     EAVDELGTTIYTNLAQVYGTKFQDEFVRANTEYFLQIALLGYIVPSVCAYDEEFKNRM
                     LELIEMRAVKTQQQMSSQSNPGGGLITP"
     gene            3822..5516
                     /locus_tag="VFJ67_03495"
     CDS             3822..5516
                     /locus_tag="VFJ67_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7289479.1"
                     /translation="MATVEGGVLVAKTLRELGVTEIFSLSGGHINPIYKACSEFGIRI
                     IDTHHEQGASMAADAYGRVGRKPGVCLVTAGPGFTNTITGIAGAYLANSPLMVISGRS
                     GVEENDRLSLQEIDQQGMAAPVTKWARTVFDTRRIPEYITSAYKKAITGRPGPVYLGM
                     SYEVLYPGCDERKVSAYNTGLPVYKPGAPVESIAKVAELLAESKRPVAVAGSGAWYSD
                     ADRALLRFIERARMPLFTLNFGRGIVSDDHPLCFGAASPSAPNAFREITGEADLILLL
                     GVRLSIYIGFGRTFNPAAKIVQIDIDPGEIGRNRPADLGLAADLATVLSELTEYMEER
                     YIESDYAAWLKRAESQWREKWKDADKLRKSSKTPIHPVRAAKAVEDVMGEDGILVIDG
                     GDTQAFTDGYFRVKLPGHYVKGGPLGCMGVGVPFAIGAKAAEPEKQVALISGDGAAGM
                     NFMEFETALRHKLPFVAVVCNDSAWGMTRHQIEITYGKGSKTQGVEFGMIPFHEVLRA
                     LGGYGELVEKPGDLEPAIERAFSSGVPALVNVVTDPDAVSGATYAITEMMMSANKR"
     gene            complement(5551..>5783)
                     /locus_tag="VFJ67_03500"
     CDS             complement(5551..>5783)
                     /locus_tag="VFJ67_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007028.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET7289480.1"
                     /translation="IPRQLYEVVIQAAIGNRIIARESVKALRKDVTAKCYGGDVTRKR
                     KLLEKQKEGKKRLKQIGKIEIPQEAFLAVLKT"
BASE COUNT         1252 a         1544 c         1657 g         1330 t
ORIGIN      
        1 tcttcctgaa cgccgacacc ggggtgtcga aatcggccag cacctccttc catacgggta
       61 tgagggtccc cttttttaac tttttcctga aatcggccag agacggtaag atcatggcaa
      121 caaagaataa aatcggcggt attaaagtca agccgcgcct ttaaatgttc ttatcttgtt
      181 gtatcatcta tatctttgtt tttcgagaat acttcggagc ttaatcacac tttttaggtg
      241 ttatccttta ttgctttcac gggcatatat attaattaca aggattggag gattcattaa
      301 tgacttacat taacccccag atattcaggg aatacgatat tagagggatc gtcgataagg
      361 acttgaacga tgacgtgctc gaacgtatag gaaaggccta cggaacctac gtaaaggaag
      421 ccggggccaa agtcgtctcc ataggcaggg actgcaggct gagctccccc gcttacgcta
      481 aagcgatgac gcggggcatc aactcgacgg gcgttgacgt gatagacatt ggcatggtct
      541 cgacccccat gctttacttt tctctttaca acctggaagt cgacggagga gtcatgataa
      601 cggcgagcca caacccgggc gagtacaacg gcataaagct ctgccggggc agggattccg
      661 tattcggcga gcagatacag aagataaggg agatcgccga ggcgggggaa ttcgcggcgg
      721 gcaaagggtc ttcgattgaa accgacatag aggagcttta cgtgggattc ctcagggaga
      781 acatacaaat aaaaccgggc gtcagggctg cggtcgatta cgggaacgga atggtgggct
      841 ccataggccc ccgggtgttc aaggaattcg gctgcgacgt caccgagctc tacgttaccc
      901 cggacggcac gttccccaac caccatcccg accccacggt agaagaaaac ctcgagaaat
      961 tgatagagac ggtgaggagc ggcagatacg agataggcat cggttttgac ggcgacgggg
     1021 acaggatagg ggtcgtcgac gagaagggca acatcgtatg gggggacatg ctcgtgctcg
     1081 tcttcgcgag ggacgtcata tcgcggaagc ctggagcgaa gatcatcggc gacgtaaaat
     1141 gctcgaaccg gctcttcaac ggcataaccg aggccggggg agaggcgata atgtggaaga
     1201 ccgggcattc cctgatcaag gataagatga agaccgagaa ggcgtcactc gccggggaga
     1261 tgagcgggca catcttcttc gccgataaat tcttcggata cgacgatgct ctctacgcct
     1321 cgctccgcat tctcgagatt atatcgagaa cgggcaagcc cctttcccga ttgctcgagg
     1381 gtatcccccc ggccatatcg acgcccgaga tcagggtcga ttgtcccgag gcctttaaat
     1441 tcaaggctgt ggaaatggtg aaagcggagt taggcggcaa atacaggatt atcgatattg
     1501 acggcgtgag ggtggagttc ccggacggat ggggcctcat cagggcgtcc aacacgcagc
     1561 cggcgctcgt attgaggttc gaggctcaga cggaggaaag gctctccgaa ataaggggta
     1621 ttatagaggg tgagctgcgg gcggctatga agaaattatc cgcctgacgg gtggatgcta
     1681 tagcacgttc tcttttgatc tgttgccgtt tacttcgtgg ttcttgagcg aaaaatatat
     1741 ccgctcgaat tcctttcgcg ttattatgcc tttctcgaaa agagcgccta cgagcgtcca
     1801 tgtgaagctc ttttcgtcta tctctacccc gttctgcctc aggagcacga gtatggcatc
     1861 gaccgcttct tgagccgagt attccccgta ttccgccctg aaattcatca tccgttcctc
     1921 cttaaagacg ctcgaagagc caacaccttt ggttcccatt cgtttatata acttatttaa
     1981 atgcgtgtca aggggttatg atacggctat gtacgaaatc aggcggcttt gatgcctaat
     2041 cgaagtatcg ggacgtcggg gagaaacggg ctttcccatc gccgtttccg gttgaccccg
     2101 gggctttggt caggcttttc cgagcctgcc gacttctctc aggatggcct cgaggctctc
     2161 gacgctgatc tcgtattctt ccgagatacg cctgagctcg cttgccggca gatcggggtc
     2221 cggcgcataa ggccttcccg ccgggacctc gggctcccag gggccttctc tcagtgaaag
     2281 ctgcgcaatg tcccagcgcg agtagtgccc gagtgaatcg aacacggcct tcacggcctt
     2341 tatctggttc gcgtggcatt ctgcgtagag tatggcgtcc ttctcgaaat ttggctcggc
     2401 caggtatctt cctgcggggt tgactatcga gctcccgccg cgcgcccagt cgtagttgat
     2461 gtgtccgccg tcccttatat aatgccactt ctctggaaaa tcgtccgagt ccagatagcc
     2521 gcatccgctc aggacgaacg ctcctgcttc gaaggcgtgt acttttatga gcgattgcaa
     2581 attgcagagc gctccgccgg ggctcgtatc ggcgtcgagc aggtggtcct cgcccctctt
     2641 ccagtttccc ggccacaccg caatgtgaaa ttcctcgccc ctgtgtatca tggcggccct
     2701 tatgagggtc atgtggtttt cccagcatat cagcccgccg atgcggccaa tgtcggtatc
     2761 gaagaccctc aagtcggacg cgtctcccat accccagtac agcctttcgg tatatgtggg
     2821 catcgtcttc ctgtgccttc cgagcacctc gccgttttta tcgataaaca agagagtgtt
     2881 atagacggtc tggctcccag gcctgtcgtc tagctcgttg caccctataa cgacatacgc
     2941 ccccgcgtcc ctggccgctt cgccgaggat acgcgtatcc tcgctcggga tgacgagcga
     3001 gttctcctgg agcgcgatca tgtaatcggt ccattcgtgg ggaggggtct cgtatccgac
     3061 cgtgtagtaa gccgggtagc acggaatgaa cgcctcggag aacgcgacga gctcggcgtt
     3121 gttccttccc gcctccctta tcagggcgca cgccttttcc acagtccgcc ccttatccat
     3181 gaaaaccggg gaggcctgca ctacggcggc ctttaccttc tctctccctc cgagaatttt
     3241 cttgctcatg ccgggcgctt atggtttacg gagttatcag acccccgccg gggttactct
     3301 gggatgacat ctgctgctgg gtttttactg ccctcatctc tatcaattca agcatcctgt
     3361 tcttgaattc ctcgtcgtac gcgcatacgg acggaacgat atatccgagc aaagcgatct
     3421 ggaggaaata ttcggtattg gcccttacga attcgtcctg gaatttcgtc ccgtaaacct
     3481 gcgcaaggtt cgtatatata gtggtcccga gctcgtctac cgcctcagtc acgctcttta
     3541 atcccccggt ctgctggttg tatgaaaccc cgaggttaaa cgccgacaat atcgagcttc
     3601 cgatgtttcc gaactcactc tgctgtccgc cccctaatgg ttgtccctga tctgtcattt
     3661 tttccctccc tgttttatca aatgtctatt ctaacacaaa ttgattttaa agccgaaagg
     3721 cctctcatgc ccggataatt ttacattcaa tcgcggattt agttgatttg gaaaaccaaa
     3781 agtgtaagag tatttagaac tctaggaccg gaggggctga aatggctaca gtagaagggg
     3841 gggtactcgt ggcgaagacg ctccgcgagc tgggagttac ggagatattt tcactcagcg
     3901 gcgggcatat caaccctata tacaaagcat gctccgaatt cggcataagg ataatcgaca
     3961 cccatcacga gcagggggcg tctatggccg ccgacgcgta cggaagggtc ggcaggaagc
     4021 ccggcgtgtg cctcgttacg gccggtccgg gtttcacgaa tacgatcacc ggcatagcag
     4081 gggcttacct cgccaactca ccgctcatgg tcatatcggg gcgctcgggt gtcgaggaga
     4141 acgacaggct gtcccttcag gagatagacc agcagggtat ggccgctccc gtcaccaagt
     4201 gggcgaggac ggtgttcgat acgaggcgca tacccgaata cataacctct gcgtataaaa
     4261 aggccatcac cgggcggccg ggtcccgttt atctgggcat gtcgtatgag gtgctttatc
     4321 cgggctgcga tgaacgcaag gtctcagctt ataacaccgg tctgcccgtt tacaaaccgg
     4381 gggctccggt ggagtcaata gcgaaagtcg cggagttgct tgcggaatcg aagaggccgg
     4441 tcgcggtcgc cgggagcggc gcatggtatt cggatgcgga tagagcgctt ctcaggttca
     4501 tcgaaagggc gaggatgccg cttttcacgc tcaacttcgg gcgaggcata gtctcggacg
     4561 accatccgct ctgtttcggg gccgcgagcc cgtcggcccc gaacgcgttc agggagatca
     4621 cgggagaggc ggacctcata ctccttttgg gcgtgaggct cagcatatac atcggcttcg
     4681 gaaggacgtt caatcccgca gcgaaaatag tccagataga tatcgacccc ggcgagatag
     4741 gcaggaacag accagctgat ttaggcttgg cagccgacct cgcgacggtg ctttcggagc
     4801 ttacggaata tatggaagag cgctatatcg agtccgatta cgcggcctgg ctgaaaaggg
     4861 cagagagcca atggcgggaa aaatggaagg acgccgataa attgaggaag tcctccaaaa
     4921 cccctataca tcccgtcagg gcggcgaagg cggtagagga tgtaatgggg gaggacggca
     4981 tactggtcat agacggcggc gacacgcagg ccttcacaga cggctatttc agggttaagt
     5041 tgcccgggca ttacgtgaaa ggcggcccgc tcgggtgcat gggcgtgggc gtgcccttcg
     5101 ctataggcgc gaaggcggcc gagccggaaa agcaggtggc tctcataagc ggcgacggcg
     5161 ccgccgggat gaatttcatg gagttcgaga ccgcgctcag gcacaagctg ccattcgtcg
     5221 cagtcgtctg caacgacagt gcgtggggga tgacaaggca ccagatcgag attacgtacg
     5281 ggaaggggag taagacccag ggcgtcgagt tcggcatgat cccctttcac gaggtgctga
     5341 gggcgcttgg cgggtacggc gagcttgtgg agaagcccgg cgatctcgag cccgcgatag
     5401 agagggcttt ttcttcgggc gtccctgcgc tcgtcaacgt cgtcacggac cccgatgctg
     5461 tgagcggggc cacgtacgcc attaccgaga tgatgatgtc cgcgaataag agataagaaa
     5521 gttcggtaga gtgtggtttg ggttgtaccg tcaggtcttg agcacggcta aaaacgcttc
     5581 ctgcggtatc tcgatcttgc cgatttgttt taagcgcttc ttcccttcct tttgcttctc
     5641 taagagcttc ctcttcctcg ttacgtcccc gccgtagcat ttggccgtca cgtcttttct
     5701 gagcgctttg accgattctc gcgcaatgat cctgttccct atcgccgcct gtataacaac
     5761 ttcatagagc tggcggggta tga
//
LOCUS       DASSJH010000162         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_469455, whole genome shotgun sequence.
ACCESSION   DASSJH010000162 DASSJH010000000
VERSION     DASSJH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469455"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1759
                     /gene="ilvD"
                     /locus_tag="VFJ67_03505"
     CDS             53..1759
                     /gene="ilvD"
                     /locus_tag="VFJ67_03505"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7289481.1"
                     /translation="MKKKEDQSAKPDKLRKFSSIVVEGADRAPNRSMLRAVGFKDKDF
                     MKPVIGIASTWSMVTPCNMHIDKLALDAAHGADKAGGKSIIFNTITVSDGISMGTPGM
                     RYSLVSREVIADSIETVAGAEGFDGLVAIGGCDKNMPGCVIAMARLNRPSVFVYGGTI
                     MPGFYNGKPVDIVSVFEAVGAHANHDISDSQLRSIETHAIPGPGSCGGMYTANTMASA
                     IEALGMSLPNSSAQAAVSEEKKNDCINAGAAVLNMIKSNIRPSDIMTRKAFENAIAVV
                     IALGGSTNSVLHLLAMANAAGVKLSIDDFTRVGRKTPVLADLKPSGRFMMADFCRIGG
                     LTPLMKMLLDEGLIHGDCLTVTGKTVKENLKDAGTYTKGQEIVRPVSDPLKKDSHIVI
                     LRGNLAPEGSVAKISGQEGLSFTGKAIVFDSEEQALKAILAGKVKKGHVIVIRYEGPR
                     GGPGMREMLSPTSAVMGKGLGKEVALITDGRFSGGTHGFVVGHITPEAYDGGAIAVIR
                     NGDTITIDAVKREISLGVAASEIKKRLTAWKKPRPKAERGALAKYAKLVSSASEGAVT
                     DQ"
     gene            2018..2743
                     /locus_tag="VFJ67_03510"
     CDS             2018..2743
                     /locus_tag="VFJ67_03510"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HET7289482.1"
                     /translation="MTTLIVSDVHLGSFYNRARELAAFIRSASFGRLIILGDLFDKPS
                     TDKLRDEEWEFLDLVRGMSRRKEIIWVEGNHDEGLYDTIPALLNIQAVKQYEWGIDGK
                     RFLAIHGHQFDIYSRNKSILASCAGELYRFLRRIDLMLGKDIFHRLCFENGAWKRSSN
                     LVTEGALEYGKNSDADFVFCGHTHRAFNVRREGVEYFNTGCWNDMHCHYVTVDSSGVS
                     LKKLSEYGEAVKTGVRLAAQQAT"
     gene            complement(2740..>3056)
                     /locus_tag="VFJ67_03515"
     CDS             complement(2740..>3056)
                     /locus_tag="VFJ67_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HET7289483.1"
                     /translation="GDGVRILVERLWPRGLSKKDAHMDIWLKDIAPSTELRKWFSHDP
                     AKWEEFRGRYFKELNKNHEAVKKLLDITERSDVTFVYGSKEKQYNSAAALKDYIENLG
                     GS"
BASE COUNT          767 a          767 c          883 g          639 t
ORIGIN      
        1 attgcgttga taataattta atcttcgtga cagcttagac aggaggctcg ttttgaaaaa
       61 gaaagaggat cagagcgcca agcctgataa attgcggaaa ttttcatcta tagtagtgga
      121 aggagccgac agggctccca accgctctat gctgagggcc gtaggtttca aggacaagga
      181 tttcatgaaa cccgtgatcg gcatagcttc gacatggagc atggtgacgc cctgcaacat
      241 gcacatagac aaactcgcgc tcgatgcggc gcacggtgcc gataaagcgg gcgggaaatc
      301 gatcattttc aacaccataa cggtttcgga cggcatatcc atgggcactc cggggatgag
      361 gtattcgctc gtttcgaggg aagtaatagc cgattcgatc gagaccgtcg cgggcgccga
      421 aggcttcgac ggcctggtcg ctatcggcgg gtgcgataag aatatgccgg ggtgcgtaat
      481 cgctatggcc cgcctcaaca ggccctcagt atttgtttac ggcggaacga tcatgccggg
      541 cttctataac ggcaagcccg tcgatatcgt gtctgttttc gaagcggtgg gcgcccatgc
      601 taatcacgat atttcggaca gccagctgag gagcatcgaa acacacgcga tacccgggcc
      661 gggatcatgc ggcggtatgt acacggcgaa tacgatggca tcggcgatcg aggcgctcgg
      721 gatgagcctt ccgaacagct cggcgcaggc cgcggtctcc gaggaaaaga aaaacgactg
      781 catcaacgcc ggggcggctg tactgaatat gataaagagc aatatacgcc cgagcgacat
      841 catgacgcgc aaggcgtttg agaacgcgat cgccgtggtc atcgctctcg gcggctcgac
      901 gaactcagtg cttcatctcc tggcgatggc taatgcggct ggcgtcaagc tctcgatcga
      961 cgattttacg cgagtcggca ggaagacccc ggtactagcc gacctcaagc cgagcggcag
     1021 gttcatgatg gccgattttt gcaggatcgg cggcctcact cctctcatga agatgcttct
     1081 cgacgagggg ctcatacacg gggactgtct tacagtgacg ggtaaaaccg tgaaggagaa
     1141 ccttaaagac gcggggacat acacgaaggg tcaggagata gtacggccgg taagtgaccc
     1201 gctcaagaag gacagccaca tcgtgatact aaggggaaac ctggctcccg aaggatcggt
     1261 cgcgaagatc tccggtcagg aagggctttc gttcacggga aaggctatcg tcttcgattc
     1321 cgaggagcag gctctcaaag cgattctcgc gggaaaggtt aaaaaaggac acgtgatagt
     1381 gatacggtac gaaggtccgc gcggtgggcc cggaatgaga gagatgctct ctccgacctc
     1441 ggcggttatg gggaagggat tgggcaagga agttgctctc atcactgacg gcaggttctc
     1501 gggaggaacg cacggattcg tcgtcgggca cattacgccc gaagcatacg acgggggtgc
     1561 cattgcggta atcaggaacg gagataccat tacaatagac gccgtaaaga gggagatcag
     1621 cctcggcgtc gccgcatccg aaataaaaaa acgcctcacg gcatggaaaa agcccagacc
     1681 caaggcggag aggggcgctc tcgcaaaata cgcgaagctc gtttcctcgg cgtcggaagg
     1741 cgctgtaact gaccaatagc taaaaaaacg ttcggaggcg tctcaaaacg cgtgggacgc
     1801 tcccgaactt actatccaac attaaattcc atatctaata attccgaaaa acagggtttt
     1861 tccctgattt tttacccttt ttaaccattt cttaagccta tcctaatact cccttaaact
     1921 gcgtgcttta taacataagc atgatgggaa attacggttt tgaggataaa gcggcaataa
     1981 gcgttacata ccaaaaaagg aggttggcgg gaaggatatg actacactaa tagtctccga
     2041 cgtgcacctg ggatcattct ataacagggc aagggagctg gcggcgttta taaggtctgc
     2101 gagcttcggc aggctgataa tcctcggtga cctgttcgat aagccgagca ccgacaagct
     2161 tcgcgacgag gagtgggagt tcctcgatct cgtaaggggc atgtcccgga ggaaggagat
     2221 aatctgggtg gaagggaatc acgacgaggg cctctacgat accattccgg ctctcctgaa
     2281 catacaggcc gtgaagcagt acgagtgggg gatcgacggg aaaaggttcc tggcgataca
     2341 cggccatcag ttcgatatat acagcaggaa caagtcgata ctcgcctcct gcgccgggga
     2401 gctgtacagg ttcctcagga gaatagacct tatgctcggg aaggacatct ttcacaggct
     2461 ctgcttcgag aacggggcgt ggaagaggtc gtcgaacctg gtgaccgagg gtgccctcga
     2521 atacggcaaa aactcagacg ccgatttcgt gttctgcgga cacacgcata gggcctttaa
     2581 tgtgaggaga gaaggggtcg agtatttcaa cacgggctgc tggaacgaca tgcactgcca
     2641 ttatgtgacc gtcgatagca gcggagtaag cctgaaaaaa ctctcggaat acggagaggc
     2701 cgtgaagacg ggcgtcaggc tcgcggcaca gcaggcgact taagatccgc cgagattttc
     2761 tatgtaatct ttcagcgccg ctgcgctgtt gtattgcttt tccttcgaac cgtagacgaa
     2821 agtaacgtcg cttcgttccg taatatccag cagcttcttt accgcctcat ggtttttatt
     2881 cagctcctta aaatatcgtc ctctgaactc ttcccacttt gcagggtcgt gagagaacca
     2941 cttgcggagc tccgttgaag gcgctatgtc cttgagccat atatccatat gagcgtcttt
     3001 cttcgacaac cccctcggcc agagcctctc gaccaggata cggactccgt cgcccg
//
LOCUS       DASSJH010000163         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1335282, whole genome shotgun sequence.
ACCESSION   DASSJH010000163 DASSJH010000000
VERSION     DASSJH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335282"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFJ67_03520"
     CDS             <1..692
                     /locus_tag="VFJ67_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET7289484.1"
                     /translation="ALLSASSYLTLHTGLFTVETIDLKGNNRITQREILKRSGLKEGQ
                     SSIFFFENQVEDEILKNPWIKKATVHKDFPKKVTIEVEEADVYCIVLSEGGKPLYMSR
                     TGRILGSGNFDLGLDFPVLIGDGIGDPKLLEEALEILELSKNSTVLKWDDISEVQLNS
                     VYGITVFTNDNRRIEFDRDNIVEKWDKAERIMRYSRSLGLEESYINVSSGSMGVVDFK
                     HRAAKPGAKDG"
     gene            685..1917
                     /gene="ftsA"
                     /locus_tag="VFJ67_03525"
     CDS             685..1917
                     /gene="ftsA"
                     /locus_tag="VFJ67_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET7289485.1"
                     /translation="MGKEQDLIIGLDIGTTKICAIVGELNSRTGEVEIIGVGTYPSYG
                     LKRGVVVNIDNTIQSVKHAVEEAEHMAGCEIRTVFVGIAGGHIKGMSGHGMITIRDRE
                     VAKRDIERVIESANAVLIPADREIIHVIPQEYTIDGQGGIKDPVGIYGIKLETKVHIV
                     TGQVTAAQNLVKCIHGAGMDVADVVLEQLASSEAVLTEDEKEIGVVLIDCGGGTTDIA
                     IFVGGSIRYTENVTLGGDNIDRDIALGLSTPLAEARRIKEKYGTALAELVSPDETIEV
                     PSVGGKHFRKISRRDLAMIIEPRMEEIFALAKREIKKSGYEDLAPAGAVITGGTVIMN
                     GTVELAERVLEMPVRMGIPTDVGGLTDIIANPVYSTGVGLVLYGSGYKGDKKLRIRDE
                     NVFKKIFDSMKNWFQEFF"
     gene            1957..>2418
                     /locus_tag="VFJ67_03530"
     CDS             1957..>2418
                     /locus_tag="VFJ67_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010530455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7289486.1"
                     /translation="MATFELEDTGIEQKAKIKVIGAGGAGGNAINAMIEKGLHGVDFI
                     VANTDSQDLRKSLASTKIQIGSRTTKGLGSGGNPVLGREAAIEDQIKIAEAIEGADMV
                     FITAGMGGGTGTGASPIIAQVSKEVGALTVGVVTRPFSFEGPKRNKQAIEGI"
BASE COUNT          684 a          545 c          725 g          464 t
ORIGIN      
        1 ttgcgcttct ctccgcgtcg tcctacctga cgctccacac tgggttgttc accgtggaaa
       61 cgatcgatct caaggggaat aacaggataa cccaaaggga gattctcaag aggtcaggcc
      121 tcaaagaagg gcagagcagc atcttttttt tcgagaatca ggtcgaggac gagatactga
      181 agaatccgtg gataaaaaag gctacagtac ataaagattt ccccaaaaag gtcacgatag
      241 aagtcgagga agcggacgtt tactgcatcg tgctttctga gggaggaaag cccctctaca
      301 tgagcaggac aggcaggatt ctcggctccg gaaattttga tctggggctt gattttccgg
      361 tgctgatagg cgatggtata ggagacccga agcttttgga agaggccctc gagatcttgg
      421 agttatctaa aaacagtact gtcctgaaat gggacgatat ttcagaggta cagcttaatt
      481 cggtatacgg gatcaccgta tttacgaatg acaaccgccg gattgaattc gatagagaca
      541 acatagtcga gaagtgggac aaggcggagc ggatcatgag gtattccagg tccctcggtc
      601 tcgaggaatc gtatatcaac gtgtcctccg ggagcatggg ggtcgtagat ttcaaacacc
      661 gtgcggcgaa accgggagcg aaggatgggt aaagagcagg atttgattat cgggctcgac
      721 attgggacga ctaaaatatg cgcaattgtg ggtgagttaa attcaagaac aggggaagtc
      781 gagataattg gcgtagggac atacccttca tacggattga aaagaggggt agtcgtaaat
      841 atagacaaca cgatacagtc ggttaagcac gcggtcgaag aagcggagca catggccgga
      901 tgcgagataa ggacggtctt cgtcgggatc gcgggcggcc acataaaagg catgagcggg
      961 cacgggatga ttactataag agaccgcgag gtcgccaagc gcgacatcga aagggtgata
     1021 gaatccgcta acgccgtgct gatccccgcg gacagggaaa taattcacgt catacctcag
     1081 gaatacacga tagatgggca gggcgggata aaagacccgg tcggtattta cggcataaag
     1141 ctcgagacca aggtgcacat tgtcacggga caggtaaccg cggcccagaa cctcgtaaaa
     1201 tgcatacacg gggccggcat ggacgttgcc gacgtcgttt tagaacagct cgcctccagc
     1261 gaggccgtgc ttacggaaga cgaaaaggag ataggcgtcg tgcttattga ctgcgggggc
     1321 ggaactaccg acatagccat atttgtcgga ggcagcatcc gctatacgga gaacgtcact
     1381 ctgggcggcg acaatataga cagggacatt gcgctcgggc tctccacgcc gctcgccgaa
     1441 gcccggcgga taaaggagaa atacggcacg gcgctcgccg agctcgtctc tcccgatgaa
     1501 acgatagaag tgcccagcgt gggcggcaaa cacttcagaa aaatatcgag acgcgatctc
     1561 gccatgataa tagagccgcg catggaagaa attttcgccc tcgcgaaaag agagataaag
     1621 aagtccgggt atgaagacct cgcccccgcg ggagcggtga taacgggcgg cacggtcata
     1681 atgaacggaa ctgtcgagct tgcggaaagg gtgctcgaaa tgcccgtaag aatgggcatt
     1741 ccgacggacg tcggaggatt gacggacatt atagcgaacc ccgtttattc aaccggagtc
     1801 ggcctcgttc tctacggcag cggttacaag ggcgacaaaa agttgcggat acgcgatgaa
     1861 aatgttttca aaaaaatatt tgacagtatg aaaaactggt ttcaggagtt tttttaggtt
     1921 aaaatgagtt aactatactg gaggagggtg ttaaatatgg caacgttcga gctagaagac
     1981 acaggtatag aacaaaaggc gaagataaag gttatcggcg cgggcggtgc gggcggtaac
     2041 gcgataaacg caatgataga gaagggtctt cacggcgtcg atttcattgt ggcgaatacc
     2101 gattcgcagg acctgagaaa atccctcgcc tccacaaaaa ttcagatcgg cagcagaact
     2161 actaaaggcc tcggctcggg cggcaacccc gtgctcggca gagaggccgc tatagaagac
     2221 cagataaaga tagccgaggc gatcgaaggc gcggacatgg tgttcatcac cgcgggcatg
     2281 ggcggaggca ccggcacagg ggcatctccc atcatagctc aggtatcgaa agaagtaggt
     2341 gcactcaccg tgggcgtcgt cacgaggccc ttcagcttcg aaggtcccaa gaggaacaag
     2401 caggctatcg aaggcata
//
LOCUS       DASSJH010000164         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1550721, whole genome shotgun sequence.
ACCESSION   DASSJH010000164 DASSJH010000000
VERSION     DASSJH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550721"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFJ67_03535"
     CDS             complement(<1..679)
                     /locus_tag="VFJ67_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289487.1"
                     /translation="MESAVSKFVFLNPPGLAVTVLCLFLFAIVIYGSLRSTRNLKSVK
                     RRVVLTSLHALAFITVIFILANPALRKESYREDKKTLAVVVDTSWSMNLSGEQEGGNR
                     AESVRSFFSDNPGYFDRLGRNYTLDYYTFDETPRASSRESVLKDKPSGRHTDFGKLIG
                     ALTEKQKRGELDEALILSDGGRSGDPGDAPSGETLKNAGLRINTVSALTADATDDIWI
                     DRINSSEV"
     gene            complement(722..1681)
                     /locus_tag="VFJ67_03540"
     CDS             complement(722..1681)
                     /locus_tag="VFJ67_03540"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7289488.1"
                     /translation="MDKLMSAAMIAVRDCMAVKEGENVLVITDEPLRKIGYALWEAAK
                     SLGAEAVITEIIPRKSNGEEPPGTVAEFMKLFDAVLIPTQKSLSHTESRRNACKAGVR
                     IATLPGITEDTMVRTLNADYGLIAERSRKLADIISRGSNVRVTTEKGTDINLVIEGRE
                     GSADTGLNHSPGDFSNLPAGEAYVAPLEGRSAGVVVFDGSMAGVGKLENEVIRAKVEG
                     GFATEITGGRGAEKLLSIMTPFGKLAFNLAELGIGTHDKALITGSVLEDEKAIGTIHI
                     AFGDNKSMGGTVRVASHLDGVVMKPTVEVDGDTIMKEGNLLVF"
     gene            1847..2845
                     /locus_tag="VFJ67_03545"
     CDS             1847..2845
                     /locus_tag="VFJ67_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7289489.1"
                     /translation="MPNIGLVAPYWSDLKTEELVETARLAEELGYHSIWVPEMWGRDA
                     FTILGLIAANTKRIKLATGIIPVFSRSPAVIAQTIATLDEISGGRMMLGLGTSGPAVI
                     ENWHGVPFDKPLQRTREYVEIIKMVLGGGRVDYEGEIFSLNGFRLQFKPLRSDIPVLV
                     AAIGPRNVRLAGETADGWIPFLVPVEGLSDSGKEFLEGARSRGRDRDKLIICPYITTA
                     VSENPDPAKDAVREHIAYYIGGMGTFYFNTVSRYGYGDEARAIRSAWEGAKKSAAIEA
                     VSDRMLDSLSVSGTPAHGKAVLAGYYESGADVPVIVFPPKASRALVRETIISLAPQ"
     gene            complement(2902..>3636)
                     /locus_tag="VFJ67_03550"
     CDS             complement(2902..>3636)
                     /locus_tag="VFJ67_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET7289490.1"
                     /translation="ETYNKDKFIKSNLRLVISIAKNYMGRGLPLADIIQEGNIGLIKA
                     VEKFDPSKGYRFSTYASWWIIQSITRSLFDQTRVIRIPVRVLEQANKITRTANMLRHE
                     NGENPDIENIAQEAGLSVKKVNKVIKATSTNIVYLDAVNPSNGEAKSSFIDFIPDTRP
                     ATDTLLAKVSMTEKIEEALSSLTDREEDILRMRFGIGYDESYTLDEIGNRYSLTRERI
                     RQIERRALKKLRQLSNGSVLKDFISC"
BASE COUNT          681 a         1088 c          987 g          880 t
ORIGIN      
        1 agacttcgct cgaatttatt ctgtctatcc atatatcatc cgtcgcgtcc gcggtgagag
       61 cgctgaccgt gtttatcctc aagcccgcat tcttaagcgt ttctccggac ggtgcgtcac
      121 cggggtctcc gctccgcccg ccgtcggaca aaatgagcgc ctcgtcgagc tcgcccctct
      181 tctgcttttc ggtgagcgcg cctatcagct ttccgaaatc ggtatgcctg cccgagggct
      241 tgtccttcag cacggactcc cgagaggagg ccctgggcgt ctcgtcgaag gtgtagtagt
      301 cgagcgtata attccggccg agcctgtcga agtaaccggg gttatcgctg aagaagctcc
      361 tcacggattc ggccctgttc ccgccttctt gctccccgga caggttcatg ctccaggacg
      421 tgtccacgac taccgcgagc gttttcttat cttccctgta actctctttt ctgagagcgg
      481 ggttcgccag tatgaatatg accgtgatga aggcgagcgc gtggagcgat gtaaggacca
      541 ccctcctttt cacgctcttg aggttcctgg tgctcctgag agagccgtaa atcactatcg
      601 cgaataagaa gaggcacagg acggttaccg caagccccgg cgggttaagg aaaacgaatt
      661 tgcttaccgc tgactccatc ggaataaatt taccacacga ctccccgctt ttcagagccg
      721 gctaaaatac gagcagatta ccctccttca ttatcgtatc tccgtccact tcgaccgtcg
      781 gtttcataac gaccccgtcc agatggctcg ccaccctcac cgtcccgccc atgctcttgt
      841 tgtctccgaa cgctatatgt atcgtcccga tagccttctc gtcctcgaga acgctcccgg
      901 ttatgagcgc cttgtcgtga gtgcctatgc cgagctccgc gaggttgaat gcgagcttcc
      961 cgaacggtgt cattattgag aggagcttct cggcccctcg tcctcccgtg atctcggtcg
     1021 cgaacccgcc ctccaccttc gccctgataa cttcgttctc gagcttcccc acccctgcca
     1081 tagagccgtc gaatacgacg accccggccg acctgccctc gagaggggcc acgtaagcct
     1141 cccccgcggg gaggttgctg aagtcgccgg ggctatggtt gagccccgtg tcggcgctcc
     1201 cctccctgcc ctcaattacg aggttgatgt cggtgccttt ctccgtggtt accctcacgt
     1261 tcgacccccg gctaataata tccgcgagct tcctgctcct ctcggcgatg agcccgtagt
     1321 cggcgttcag cgtcctcacc atcgtgtcct ccgttatccc cgggagcgtc gctatcctca
     1381 ctcccgcctt gcacgcgttc ctcctcgact cggtgtggct gagggacttc tgtgtcggta
     1441 tcagcacggc atcgaacagc ttcatgaatt cggcgaccgt cccgggcggc tcctccccgt
     1501 tcgatttgcg gggtattatc tcggtgatga cggcttccgc cccgaggctc tttgccgctt
     1561 cccagagcgc atacccgatc tttctcagcg gctcgtcggt gatgacgagc acgttctctc
     1621 cctcctttac agccatgcag tccctgaccg cgatcatcgc ggcgctcatc agcttgtcca
     1681 tgtcccgtgt ctcctcgctc cgttcgttta ttgtgcgtaa aaaatccgat agtataatat
     1741 gccgatcccg gagcgccctg taaatgcgca cgggacggag cctctcgcga aatgcccgat
     1801 accgactcac gcgatatttt caacctacat acatgcgggg gtgatcatgc ctaatatcgg
     1861 tctcgtggct ccatactgga gcgacctcaa aactgaagag ctcgtcgaga cggcccggct
     1921 cgcggaagag ctcgggtacc attcgatatg ggtcccggag atgtggggaa gagacgcgtt
     1981 tactatactc ggcctgatcg cggctaacac gaagaggata aagctcgcta ctgggataat
     2041 ccccgttttc agcaggagcc cagccgtcat agcgcagacg atagcaacgc tggacgagat
     2101 ttcgggcggc cgcatgatgc tcggcctggg aacgagcggc ccggcggtta tagagaactg
     2161 gcacggcgtt cccttcgata agccccttca gcggacgagg gaatacgtgg agataatcaa
     2221 gatggtcctc ggcggaggac gggtcgacta cgaaggcgag atattctcgc tcaatgggtt
     2281 caggctccag ttcaaaccgt taaggagcga tatacctgtt ctcgttgcgg ctattggccc
     2341 caggaacgtg cggctcgcgg gagagacggc cgacggctgg atacctttcc tcgttccggt
     2401 cgagggcctg agtgattccg gtaaagagtt cctggaagga gcccgctcga ggggcaggga
     2461 tagggataag ctcataattt gtccgtatat cacgaccgcg gtttcggaga accctgatcc
     2521 ggcgaaggac gccgtaaggg agcacatcgc atactacatc ggagggatgg ggacttttta
     2581 tttcaatacg gtgagcaggt acgggtatgg ggatgaagcc cgggcgataa gaagcgcgtg
     2641 ggaaggcgcg aagaagagcg ccgcgataga agccgtgagc gacaggatgc tcgactcttt
     2701 atcggtctcg ggtacgcccg cccacggaaa ggccgttctg gccggttact atgagtcagg
     2761 cgccgacgtc ccggtgattg tttttccgcc taaagcctcg cgagccctgg tcagggaaac
     2821 tataataagc ctcgccccgc aataatctgc ttattgctcg tatatagagc gtgagagacg
     2881 ggctcttccg aaacgcacgc ctcagcagct tataaaatct ttcagcacac tgccgttgct
     2941 gagctgtctc agcttcttga gcgcgcgtct ctcgatctgc ctgatgcgtt ccctagtgag
     3001 gctgtacctg ttcccgatct cgtcgagcgt gtaggactcg tcgtatccga tgccgaacct
     3061 catcctgagt atgtcttcct ccctgtcggt caggctcgag agcgcctctt cgatcttctc
     3121 cgtcatggat accttcgcga ggagcgtgtc cgtcgcgggt ctcgtatccg gaataaagtc
     3181 tatgaagctg ctcttggcct ccccgttgct ggggttaacg gcgtcgagat aaacgatgtt
     3241 agttgaagtc gcttttatta ccttgttgac tttctttacg ctgagacccg cttcctgcgc
     3301 gatattctct atgtccgggt tctcgccgtt ctcgtgacgg agcatgttcg cggtcctcgt
     3361 gatcttgttc gcctgctcga gcacccgcac cggaattcgt atcacccttg tctggtcgaa
     3421 cagagagcgc gtaatgctct ggattatcca ccaggaagcg tatgtcgaaa acctgtagcc
     3481 cttcgaaggg tcgaactttt cgacggcctt tataagccct atattgcctt cctgaattat
     3541 atcggctagc ggtagtcctc tccccatgta gttcttcgcg atgcttatca cgagcctgag
     3601 gttcgatttt atgaatttat ccttgttgta agtctc
//
LOCUS       DASSJH010000165         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1225819, whole genome shotgun sequence.
ACCESSION   DASSJH010000165 DASSJH010000000
VERSION     DASSJH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225819"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1481
                     /locus_tag="VFJ67_03555"
     CDS             165..1481
                     /locus_tag="VFJ67_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7289491.1"
                     /translation="MRTHNIFIAALLLSLLFGTINSPGHEDGHSHQDDKTQLRESPAD
                     GLIELYKSKISEDPDDYYNYTKLGESYIQKGREVGGIKPYHQAEQALKKAVELYPDGY
                     AAYIYLGQVSSYTHNFQKTIEYAKKAIELKPEKSVPYGLLGDAYMELGKYEEAGKAYQ
                     IASIISPGFYSLSRISQLKDLTGDTNGAILAMKESVEMAESGNLPVENRAWANVMLGS
                     LYLKEGKIEEAEGYYEKALAIFQDYYLALGQLAQLHTESGEYDEAAKLYERAIELNPR
                     PQYYLALGKVYDELGIPEKSKASYRKAEEGYQEYAKSGIRGHSRELALYYADNGINLE
                     RALELAKRDSADTEDIYAFDTLAWAYYKTGNIDKARDAINKSLRLGTKDAVLYYHAGM
                     IYYKLDKNEEAEKYFSLVLTTNPFFDKDAVNNVRYALGELGKSVSR"
     gene            1703..2122
                     /locus_tag="VFJ67_03560"
     CDS             1703..2122
                     /locus_tag="VFJ67_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289492.1"
                     /translation="MENGHSNGKEIKLGQLTQDPGVNINVLRRSTRIPYHMRLKYSLL
                     NSKSSEDSEMHLFSGSTFNISEHGIGMEGTKGFPPNFKIYATLFTGAKVINFEGTIKW
                     LYHSNSEKWYMGVEISSRRDELKEIYATLSQLSNPGM"
     gene            complement(2137..>2443)
                     /locus_tag="VFJ67_03565"
     CDS             complement(2137..>2443)
                     /locus_tag="VFJ67_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289493.1"
                     /translation="ATVAVMATAFAILPKKRRDAMKEFSSKVDALIEEMISGVSSQLE
                     RDFEGIKIQVNDSLSPLRNFYKMEKQKLSESKERVNELKSGFAEIKDKMLRSQSVRS"
BASE COUNT          727 a          553 c          614 g          549 t
ORIGIN      
        1 atccgggcga gaggggatgc aggggtacgg atacgaaacg ctgtcctgta gattaaggat
       61 ttatgaggat gacggcaaga gggggatgtt tatatactcc ccctcgtctc tatttttcga
      121 atataagata gactcagaaa aaatatgatt aaggtgcagg aactatgaga actcacaata
      181 tatttattgc cgcattactt ctttcgctgc tattcggaac cattaactcc cctggacatg
      241 aagacggtca ttctcaccag gatgataaaa cacaattaag agagagtccc gcagacggtc
      301 ttatagagct ttataaatcc aaaataagtg aagaccccga tgattattat aactacacaa
      361 aactcggaga gagctacatt cagaagggga gagaagtggg aggtataaag ccctatcatc
      421 aagccgaaca ggcgctcaag aaagccgtcg agctttatcc cgacggatac gctgcgtata
      481 tatatctggg tcaggtgagc tcatacacgc ataattttca aaaaactatt gaatacgcga
      541 agaaagcgat cgagttaaaa cccgaaaaga gcgttccgta cggccttcta ggcgacgcgt
      601 acatggagct cggaaaatac gaggaggcag gtaaagcata ccaaatcgcc tcgataatat
      661 ctccgggatt ctattcatta agccgcatct ctcagctgaa ggatttgacg ggggatacca
      721 atggcgcgat tctggcgatg aaggagtcag tagagatggc ggagagcggc aatcttcccg
      781 tagaaaacag ggcatgggca aacgtaatgc tcggctctct atatttgaag gaagggaaga
      841 tagaggaagc cgaaggttat tatgaaaaag cgctcgcgat ttttcaggat tactacctcg
      901 cgctcgggca gctagcacag cttcacacag aaagcgggga atacgatgaa gccgcaaagc
      961 tttacgaaag agcgatcgaa ttaaacccga gaccgcaata ctacctggcg ctcggcaagg
     1021 tatacgatga gctcggcata ccggagaaat ccaaagcatc atatcgcaaa gccgaggagg
     1081 gatatcaaga gtatgcgaaa agcggaatca ggggacattc gagggaacta gcgctttact
     1141 atgcggacaa cggtataaac ttggagcggg ctttggagct ggcaaaaaga gattccgcgg
     1201 atacggagga tatttacgcc tttgatactc tggcctgggc ttattataaa acagggaata
     1261 tcgataaggc gagagacgct ataaataaat cattaaggct gggaacgaag gatgccgttc
     1321 tttattatca cgccggaatg atttactaca aactcgacaa gaatgaggag gccgaaaaat
     1381 atttcagcct cgtcctgacg acgaatccgt ttttcgataa ggatgctgta aacaatgtca
     1441 ggtacgcact cggcgagctc ggcaaatcgg tctcccggta aatgtccgtc aaacccggtt
     1501 tcgcgacaag ccggtgtgag aattgcacat aatgtccgca gccgggccgg gcattacggc
     1561 ctgacaacag catcataagt cattgaatcg gcacgaatac acggtatccg agtttaattt
     1621 cagaacacag gacgaaagtt ttaagccatt tacagaccaa agtccaattg aaataccggc
     1681 gaatcaatga tattttaatc ggatggagaa cggtcattcg aacggtaagg aaataaagct
     1741 cggccagctc actcaggatc ccggtgtcaa tataaacgta ctcaggcgct cgacgcggat
     1801 accatatcat atgaggctga agtacagcct actgaacagc aaaagcagtg aagacagtga
     1861 gatgcatctg ttcagcgggt ccactttcaa catctccgag cacggtatcg ggatggaagg
     1921 cactaaagga ttcccgccca atttcaaaat atacgctacg ctgttcacag gggcaaaggt
     1981 aataaatttc gaaggaacta tcaaatggct ctatcactcg aactccgaga aatggtacat
     2041 gggggtcgag atctcgagcc ggagggacga gctgaaagaa atatatgcga ccctctcaca
     2101 gttgagcaat cccggaatgt aacggacggt tttaggtcag ctccgcacgc tctgacttct
     2161 cagcatctta tcttttatct cggcgaaccc ggacttgagc tcgttcacgc gctctttaga
     2221 ttcggataat ttttgcttct ccatcttgta gaaatttctg agcggagaga gtgagtcgtt
     2281 aacctggatc ttgataccct cgaagtccct ctcgagctgc gagcttacgc ccgatatcat
     2341 ctcctctata agcgcgtcca ccttactgct gaactctttc atggcgtccc tcctcttctt
     2401 ggggaggatc gcgaaagcgg tcgccatcac cgcgaccgtc gca
//
LOCUS       DASSJH010000166         6135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1445150, whole genome shotgun sequence.
ACCESSION   DASSJH010000166 DASSJH010000000
VERSION     DASSJH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445150"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2741
                     /locus_tag="VFJ67_03570"
     CDS             <1..2741
                     /locus_tag="VFJ67_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7289494.1"
                     /translation="GMTVSDVVQSLREQNVEVAAGVLGQPPVPSGNAYQLSVNTLGRL
                     DEADQFSEVVIRTGEDGRITHLKDVARIELGARDYGVNSYLDGKPAQAIGIFQLPGSN
                     ALATAQAVEKTMARISKDFPKGIEYRIIYNPTVFVQQSINEVYHTLFVAFVLVFIVVI
                     VFLQDWRAAILPMIDAVVSLVGTFAVMAAFGFSLNNLSMFGLVLAIGIVVDDSIVVVE
                     NIKRWMSKGLERREATLKAMAEITGPVLAITLVLSSVFIPTAFLPGISGQFYKQFALV
                     IASSTIISAINALTLAPARAVQLITPDTDAEHHEPLPRVGIAAILGFLAYWLLTGPVT
                     GIFGIHTVQGYGPEQSSTAALWAVRIVLFAAGCAAGWFAGPFVNRVLSKVFGGFNRLF
                     DRASEMYSNSVSRLLRFSAIAIIVYCGLIGLTYLGFTRVPSGFIPAQDQGYLIVDVAL
                     PDGASLERTDAVIARATNIILDTPGIAYAVGFAGFSGATFSNSSDAGVIFTPLAPFDQ
                     RARHGPKLQEIIADLSKRLSVIQEARIIVISPPPVRGLGTAGGFKMEVQDRGDVGLRE
                     LEKATYALIGAANQEPGLTRVFTTFSTRTPQIYAEVDRTKAKKLDVPLTNIFDTLQVY
                     LGSVYVNDLNLFGRVYRVTAQAEGGFRQQPEDIAKLRTRNARGDAIPLGSVVDIKSET
                     GPDRVVRYNLYPAIEINGDTLPGFSSGQAIAAMEKLAKKILPAGVSFEWTDIAYQQVT
                     AGNTALYIFPLCVLFVFLILSSLYESWSLPLAIILIVPMCLLSALTGLMIRGMDNNIL
                     TQIGFVVLVGLACKNAILIVEFAKDYQETGKGREEAVVEASRVRLRPILMTSFAFILG
                     VVPLLIAAGAGAEMRQALGTSVFSGMFGVTFFGLFLTPVFYVVIRRISESRKSGAK"
     gene            2746..4206
                     /locus_tag="VFJ67_03575"
     CDS             2746..4206
                     /locus_tag="VFJ67_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET7289495.1"
                     /translation="MRFISAILLTTILLASAGCTVGPDYKTPQTDLPESFQDSGAKDF
                     STDSIEIEWWSGFSDPVLDKLIQDALAGSYDIQIATARILEARALRTETEFQLAPIVP
                     FNASYTKQQVGESTVFPGVKREADLYEAGFDAVWELDFFGRIRRTIEADTAEVQSREA
                     QRRDVIVSLLAEVARNYFELRGAQNQLDVARRNAANQQNTLDLTITLLEGGRGTELDT
                     SRARAQLNNTLAIIPPLEARVRTTIYRLGVLTGRQPDAYLEELLVPKPFPKVPDVIRI
                     GTPVGLLERRPDIRVAERNLAGFTASIGIATAELFPQITLLGSISLQADTFSGVFESG
                     AGAFSIGPNIFWPAFDLGRVYQRVKQADARTQAAFAEYQLTVLQAIEDVDSSLVEHNQ
                     QIVRYKYLAEAAVDSEKAMKLARMRYDFGVASFLDVLDAERTLLQAQTQLAASETDLH
                     TSLVAVYKSLGGGWEIYDENMAETSANVSAGGDQQK"
     gene            complement(4230..4598)
                     /locus_tag="VFJ67_03580"
     CDS             complement(4230..4598)
                     /locus_tag="VFJ67_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289496.1"
                     /translation="MGKFNIVLSVFGGMILAVLLLLSFVIPYVILPIAAALGSLVLVS
                     IYFLKEDRSLSDRAAMKTFYCPFRKMIVEAKLRPSIFAYRTYDDVLKCSAFKDRVRCQ
                     KRCLNMPEFKSDPRPVAVLH"
     gene            4942..5703
                     /gene="cmoA"
                     /locus_tag="VFJ67_03585"
     CDS             4942..5703
                     /gene="cmoA"
                     /locus_tag="VFJ67_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HET7289497.1"
                     /translation="MKRDRVFREQKGMTADFEFNDTVADVFDDMVVRSVPFYSEQQRM
                     IKEIGGIFSTPGSNIYDLGCATATTLINLCREVDPSASLIGYDNSAPMIEKAKLKIKE
                     NGFEDRIQVLYADLNGDLDSIELNNASVVIICWTLQFVRPLKREKLINKIYNGLNENG
                     VLIITEKILTNSSLLNRYYIKLYYDYKQRNQYTYEEITNKREALENILIPYRIDENIE
                     MFKRNGFETVDTFFQWYNFVGFICIKSRNPIHDNF"
     gene            5741..>6135
                     /locus_tag="VFJ67_03590"
     CDS             5741..>6135
                     /locus_tag="VFJ67_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7289498.1"
                     /translation="MNIRSLLKKSVELVRSRISSIWSRYYEIPGSVSPVSRTVGYDRG
                     QPIDRYYIEKFLSAHSSDIKGRVLEFGNRNYTSRYGGDKVVKSDVLCIEEGNPEATIV
                     ADLTGAHQIQSNTFDCIIMTQTLQMIYDF"
BASE COUNT         1428 a         1740 c         1737 g         1230 t
ORIGIN      
        1 tgggcatgac cgtgagcgac gtcgtgcagt ccctgagaga gcagaacgtc gaagtagcgg
       61 ccggcgttct cgggcagccc ccggtgccgt cgggcaacgc ttaccagctt tcggtcaata
      121 cgctcggaag gctcgacgag gccgaccagt tctcagaagt tgtcatcagg acgggcgagg
      181 acgggcgtat cactcacctg aaggacgtag cgcgcataga gctaggagcg agggattacg
      241 gcgtcaacag ctacctcgac ggcaagcccg cgcaggcgat cggtatattc cagctccccg
      301 ggtcaaacgc gctcgccaca gcccaggccg tggaaaagac catggcccgt atcagcaagg
      361 acttccccaa ggggatcgag tacaggataa tctataaccc gaccgtattc gtacagcagt
      421 ccataaacga ggtctatcac acgctgttcg tggcgttcgt cctcgtgttc atcgtagtaa
      481 tcgtattcct tcaggactgg agggccgcga tcctgcccat gatagacgcg gtagtgtccc
      541 tcgtcggcac tttcgcggtg atggcggctt ttggattttc actcaataac ctatcgatgt
      601 tcggcctcgt gctcgcgata ggtatcgtcg tcgacgattc cattgtcgtc gtagagaaca
      661 tcaagcgctg gatgtcgaaa gggctcgagc ggagggaggc gacgctgaaa gccatggctg
      721 agatcacggg ccccgtgctc gcgatcacgc tcgtcctgag ctcggtattc ataccgacgg
      781 ctttcctccc tggcattagc ggccagttct ataagcagtt cgcactcgtt atcgcctcat
      841 cgaccatcat ctcggccata aacgcgctga cactcgcccc ggcgagggcg gtgcagctca
      901 tcacgcccga caccgatgcc gagcatcacg agcccctgcc ccgcgtcggc atcgcggcca
      961 tactcggctt tttggcgtac tggctgctca ccggtcccgt cacggggatc ttcggaattc
     1021 ataccgtaca gggctacggt ccagagcagt cgagcacggc ggccctctgg gccgttcgaa
     1081 tagtgctctt cgcggcagga tgcgcggcag gatggttcgc gggccctttc gtcaacagag
     1141 tgttaagcaa ggtgttcggc ggtttcaaca ggctgttcga ccgcgcatca gagatgtaca
     1201 gtaactcagt cagcaggctg ctgcgcttct cggccattgc cataatcgtc tattgcggtc
     1261 tgataggact tacatacctc ggattcacga gggttccgag cgggtttatc ccggcgcaag
     1321 accagggtta tctcatcgtc gacgtcgccc ttccggacgg cgcttccctc gagcggacgg
     1381 acgccgtgat cgcacgcgcg acgaacatca tcctcgacac ccccggcata gcctacgccg
     1441 tcgggttcgc cggcttctcg ggagccacgt tctcgaacag ctcggacgcg ggcgttatat
     1501 tcaccccgct ggctccgttc gaccagcgcg ccaggcacgg ccccaagctc caggagataa
     1561 tagccgactt aagcaagagg ctttctgtca tacaggaggc gaggatcata gtcatctcac
     1621 cgccgccagt caggggactt ggtactgcgg gcgggttcaa gatggaggtc caggaccgcg
     1681 gcgacgtcgg tctcagggag ctggagaagg cgacctacgc gctgataggg gccgcgaacc
     1741 aggagcccgg actgacgagg gtattcacga cgttcagcac gaggacaccg cagatatacg
     1801 ccgaggtcga caggaccaag gccaagaaac tcgacgtccc gctcaccaat atattcgaca
     1861 cgctccaggt gtatctcggc tctgtttacg taaacgacct caacctcttc ggacgcgtct
     1921 acagggtgac ggcgcaggcg gaggggggat tccgtcagca gcccgaggac atcgcgaagc
     1981 tgaggacgcg gaacgccagg ggggatgcca taccgctcgg ctccgtcgtt gacataaaga
     2041 gcgaaacggg acccgaccgc gtcgtgcggt ataacctcta tcccgcgata gagataaacg
     2101 gcgacacgct gcccggcttc agctcggggc aggcgatagc cgcgatggag aagctcgcga
     2161 agaagattct gcccgcgggc gtgagtttcg agtggacgga catcgcttac cagcaggtga
     2221 ccgccggaaa cactgcgctc tacatattcc cgctctgcgt gcttttcgta ttcctcatac
     2281 tttcctctct ctatgagagc tggtcgctcc ctctcgcgat catactgata gtgcccatgt
     2341 gtctcctgtc cgcgttaacg ggcctgatga tccggggcat ggacaacaac atcctcacgc
     2401 agatagggtt cgtcgtgctc gtgggtctcg cgtgtaagaa cgcgatattg atagtcgagt
     2461 tcgccaagga ctatcaggag acgggcaagg gccgcgagga ggccgtggtc gaggcctcgc
     2521 gcgtaaggct ccgccccatt ctcatgacct cgttcgcttt catactgggc gtggtgcccc
     2581 ttctaatagc ggcgggcgcg ggcgccgaga tgcgccaggc gctcggaacg tccgtgttca
     2641 gcggcatgtt cggggtcacg ttcttcggcc tctttttaac gccagtcttc tacgtcgtca
     2701 taaggcgtat atcagaatcc agaaaatccg gagcgaagta gagtcatgag attcatatcg
     2761 gcaattttac tgacaaccat tctgcttgcg tccgcgggct gtacggtcgg ccccgactac
     2821 aagaccccgc agacggacct ccccgaatct tttcaagact cgggcgcgaa ggacttctcc
     2881 acggactcga tcgagatcga gtggtggagc gggttcagcg acccggtcct cgacaagctg
     2941 atccaggacg cgctcgccgg gagctacgac atacagatcg cgaccgcgcg catcctggaa
     3001 gcgcgcgccc tgaggacgga gaccgagttc cagctcgcgc ctatcgtgcc tttcaacgcg
     3061 agctacacga agcagcaggt cggggaatcc acggtcttcc ccggtgttaa gagggaggca
     3121 gacctctacg aggcggggtt cgacgccgta tgggagctcg acttcttcgg gcgcatcagg
     3181 cggacgatcg aggccgacac ggccgaggtc cagtcccgcg aagcccagag gcgggacgtg
     3241 atagtgtccc ttctggccga ggtcgcgagg aactatttcg agctgagggg agcgcagaac
     3301 cagctcgacg tcgcgcgccg gaacgcggcc aaccagcaga acacgctcga cctcaccatc
     3361 acgcttctcg aaggcggcag gggcactgag ctcgacacct cgagagcgag ggcgcagctc
     3421 aacaacacgc tcgccatcat accgccgctc gaagcgaggg tcaggactac catatacagg
     3481 ctcggggtgc tgacgggacg tcagcccgac gcgtacctgg aagagctcct cgtgcccaaa
     3541 ccgttcccca aggtgcccga cgtcatcagg atcgggaccc cggtcggact tctcgaaagg
     3601 aggccagaca tcagggtcgc ggagaggaac ctcgccgggt tcacggcgtc cataggcata
     3661 gccacggccg agctcttccc gcagataacg ctcctcggta gcatctcgct ccaggctgat
     3721 acgttctcgg gcgtcttcga gagcggggca ggagctttct ccatagggcc gaacatattc
     3781 tggccggcct tcgacctggg tcgggtgtac cagagggtaa agcaagccga cgcgcgcaca
     3841 caggcggcgt tcgctgagta tcagctcacg gtgctacagg caatcgagga cgtggacagc
     3901 tcgctggtcg agcacaacca gcagatagtc aggtacaagt acctggccga agccgcggtc
     3961 gacagcgaga aggccatgaa gctcgcgagg atgaggtacg acttcggcgt cgccagcttc
     4021 ctcgacgttc tcgacgccga gcgcacgctc cttcaggcgc agacgcagct cgccgcgagc
     4081 gagacagacc tccacacctc cctcgtcgcc gtttacaagt ctctcggcgg cggatgggaa
     4141 atatacgacg aaaacatggc cgagacctcg gctaacgttt cagctggcgg cgatcagcaa
     4201 aagtaacaca caccggtgtc agttcctgat caatgcagga cagccacagg ccgcggatcg
     4261 gatttgaact ctggcatgtt caggcatcgt ttctggcatc tcaccctgtc cttgaacgcc
     4321 gagcatttca gcacgtcgtc gtatgtccta tatgcgaaaa tactcggccg cagcttggct
     4381 tccacgatca ttttgcggaa cggacagtag aacgtcttca tcgcagctct gtccgacagg
     4441 cttcggtctt ctttaagaaa atagattgat acgaggacga gcgaaccaag cgctgcggct
     4501 atgggcagaa tgacgtacgg tatcacgaac gagagcagga gcaaaacggc cagtatcatg
     4561 ccgccgaata cggaaagaac tatattgaat ttacccatga tacaccccca ttcaatatat
     4621 tttataatga attaggttgc ataatttatg ccgcgggcat gcggcgatac aagccgaaaa
     4681 aactggttat tttttatggg cattttgaga aacgtccgga ttgtccgacc ttctgacaca
     4741 tttcccaaat tgcccctccc ggcggaagca aacccaagtg acggggataa tcggcggcgg
     4801 gagcaggatt cgagactgac gcaatagggt tattggtgct attctaaatg ttgtaagggt
     4861 ttgccggaat ttgcataatt gaaataaaag ataaaatatc aactgggttc aaataaacaa
     4921 ctgactctaa gaggcagcta tatgaagcgc gacagggtat ttagagaaca aaaaggcatg
     4981 accgccgatt ttgagtttaa tgatacggtc gccgacgtct tcgacgacat ggtcgtccgg
     5041 agcgtcccgt tctactcgga gcagcagagg atgataaaag agatcggcgg catattctca
     5101 acacccggca gcaacatata tgatctcgga tgcgccactg ccactaccct cattaatcta
     5161 tgccgggaag tcgatccgtc ggcctctctg atcggctacg ataattccgc gcctatgata
     5221 gagaaagcga aactcaagat aaaagagaac ggattcgagg atcgtatcca ggtgctttat
     5281 gcggacctca acggtgacct cgattcaatt gaactgaata atgcgagcgt tgtaataata
     5341 tgctggactc tgcaattcgt ccgcccgtta aaaagagaaa agctcataaa caaaatatac
     5401 aacgggctca atgaaaacgg cgttcttata atcactgaaa agattcttac aaacagctcg
     5461 ctcctcaacc gttactacat caagctctat tatgattaca agcagcgtaa ccaatacacg
     5521 tatgaggaaa taacgaacaa gagggaagcg ctcgagaaca ttctaatccc ctacaggatc
     5581 gacgaaaaca tcgagatgtt taaacggaac ggttttgaaa ccgtcgatac cttttttcag
     5641 tggtataatt tcgtcgggtt catctgcata aagagccgga atccgatcca tgacaacttt
     5701 taacgatccc gaataccggg catcaacctg acgcctcctt atgaacatcc ggtcccttct
     5761 taaaaaatca gtggagctcg tcaggagccg aatcagctcc atatggagca ggtattatga
     5821 gattcccggg agcgtatcgc ccgtaagcag gaccgtcggc tacgacaggg ggcagcccat
     5881 cgaccgttat tatattgaaa agttcctgag cgcccattct tccgacatta agggacgcgt
     5941 gctcgaattc gggaacagga attacacatc gagatacggg ggagacaagg tcgtaaaaag
     6001 cgatgtcttg tgtatcgaag aagggaatcc cgaagcgacg attgtagccg atctgacggg
     6061 cgcgcatcaa atacaatcaa acacctttga ttgtattatc atgactcaaa cgctgcaaat
     6121 gatctacgat ttcca
//
LOCUS       DASSJH010000167         3678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1551754, whole genome shotgun sequence.
ACCESSION   DASSJH010000167 DASSJH010000000
VERSION     DASSJH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551754"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1664
                     /gene="rpoD"
                     /locus_tag="VFJ67_03595"
     CDS             75..1664
                     /gene="rpoD"
                     /locus_tag="VFJ67_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET7289499.1"
                     /translation="MRNKKKDSFQMKIVDEERVPDTTVDSIDEDKIDVDIVFTVEQDD
                     EEFIEEEVQESAVKYGFNLNDAKENASEYDLIRFYLHEIADHSLLTREQEIRIAKEIE
                     TGKRIVAKAILGSPVMLKEIIRLGDEIQKGSLNVRDITNVLDDADNAVEEELQLGIRN
                     SLTAINKLHEDNEQANKELGPASVRKKKVLLERIKKNNGRMLGYLEDINLNATQMERI
                     LSVARRDIDRQEKLRRESETLKNRKKPAVSKSRKSPKGKVEAIFTDPGPDTGLLSKAL
                     KRIEFGEQMTHRARRKLIESNLRLVVSIARRYINRGLPFLDLIQEGNMGLMRAVEKFE
                     YNRGYKFSTYATWWIRQAITRALADQSRIIRIPVHMTETINRIVRTSRHLVQELGREP
                     LPEEIADKVGMQSDKVARVLKISKDPISLETPIGDEEDSKLVDLIEDSNTTSPVKVLE
                     MSELKEIINHALASVLNIREESIVRLRFGIDDEKEHTLEEVGHEFKVTRERIRQIEVK
                     AIKKLKRAGRVYPIKSYLEKA"
     gene            complement(1699..2580)
                     /locus_tag="VFJ67_03600"
     CDS             complement(1699..2580)
                     /locus_tag="VFJ67_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7289500.1"
                     /translation="MIKFSVMEELIRAFIGSLRRRETTKETYRKALREFSKWLGGTSP
                     SVMTPDDIQRYKDYIISKRLSATSMSSYLTAVRRFYDYLVSTGTVRENPARKVKGAPR
                     PRRHLTDPITRRDVSKLMNAVDKSSPLGRRDAAILGLMVRAGLSEIEIVRLNLGDLKY
                     KGGQRVIYVQGKSKDRKDEYVSVAQPMEETLMKYLEERGESGPEEPLFRGIGNRAVKE
                     RISTRGLRARINHYFESSGIKKSGITPASLRHTAAMLAIEEGATVSEVQRLLRLRTSE
                     SALQYFEEAKELMRKQF"
     gene            2649..2765
                     /gene="rrf"
                     /locus_tag="VFJ67_03605"
     rRNA            2649..2765
                     /gene="rrf"
                     /locus_tag="VFJ67_03605"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            2906..3508
                     /gene="purN"
                     /locus_tag="VFJ67_03610"
     CDS             2906..3508
                     /gene="purN"
                     /locus_tag="VFJ67_03610"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET7289501.1"
                     /translation="MKKTRLAVFVSGGGTNLQAIIDARIPSVDIVLVFSNNPGAYALE
                     RASNEGIPCEVLDHKKYASRDEYDSEALELIEPYDIDLIALAGFMRILSPRFVRAYQN
                     RILNIHPALLPSFPGVNAARQALEAGVKHTGVTVHFVDEGVDTGPIILQSVVPVLDDD
                     TEESLLERIHSEEHRIYPAAIELVASGKFRIEGKRVIALE"
     gene            complement(3526..>3678)
                     /locus_tag="VFJ67_03615"
     CDS             complement(3526..>3678)
                     /locus_tag="VFJ67_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7289502.1"
                     /translation="IAAGADGLIIEVHNNPEVAISDGGQSLKPKKFAALMEKAAPVAK
                     AVGREI"
BASE COUNT          924 a          921 c          998 g          835 t
ORIGIN      
        1 agagcttgaa aagcggctat tgactgaata caccgattta atgaaacaaa aataaccgga
       61 agaggggaga ccctatgcga aataagaaga aagacagttt tcaaatgaag atagtcgatg
      121 aagaaagggt gcctgatacg accgttgatt cgatagacga ggataagatc gacgtggata
      181 ttgtctttac ggttgagcag gacgatgagg agtttatcga agaggaagtc caggaaagcg
      241 ctgtaaagta cggattcaat ctgaatgacg cgaaagagaa cgcgagcgaa tacgatctta
      301 tcaggttcta tcttcacgaa atagcggacc attcgcttct tacgagggaa caggagataa
      361 ggatcgcgaa agagatcgag acgggcaaga ggatcgtcgc caaggccata ctcgggtctc
      421 ccgttatgct gaaggagata ataaggctgg gcgacgagat tcagaaaggc agccttaacg
      481 tgagagacat caccaacgtt ctcgacgacg ccgataacgc tgtcgaggaa gagctccaac
      541 tgggtatcag aaattcattg acggccataa ataaattaca cgaggataat gagcaggcta
      601 ataaggagct cggcccggca tccgtcagga aaaagaaagt ccttctcgag agaataaaga
      661 agaacaacgg caggatgctg ggatacctgg aagacattaa cctgaacgct actcaaatgg
      721 agcggatact gtcggtcgcc aggagggaca tagacagaca ggagaagctg aggcgcgaat
      781 cggagactct gaagaacagg aaaaagcccg cggtatccaa gtcccggaaa tctcccaaag
      841 ggaaggtcga agcgattttc accgatcccg gcccggatac aggtttactg agcaaggctc
      901 tcaaaaggat agagttcggc gaacagatga cacacagggc caggaggaag ctcatcgaat
      961 ccaacctgcg tctcgtcgtc agcatcgcca ggcgttatat caaccgcggt ctgccgtttc
     1021 tcgacctcat acaggaggga aacatggggc tgatgagggc ggtcgagaag ttcgaataca
     1081 acaggggtta taaattttcc acttatgcga catggtggat aagacaggcg ataactaggg
     1141 cgcttgcgga tcagtcgagg attatcagga ttccggtgca catgacggag accataaaca
     1201 ggatagtcag gacttcgaga cacctcgtgc aggagctcgg tcgagaacct ctgcccgaag
     1261 agatcgcgga caaggtcggc atgcagtcgg acaaggtcgc gcgggtgctt aagatatcga
     1321 aggaccctat atcgctcgaa actcccatcg gcgacgagga ggacagcaag ctcgtcgacc
     1381 tgatcgagga ctcgaacacc acgtctccgg tcaaggtgct cgaaatgagt gagctcaaag
     1441 agataatcaa tcacgcgctt gcttccgtgc tcaatatcag ggaggagagt atcgtgaggc
     1501 tcaggttcgg catagacgac gaaaaagagc atactctcga agaggtcggg cacgaattca
     1561 aggttacgcg tgaaaggata aggcagatcg aagttaaagc gataaagaag cttaaaaggg
     1621 cagggcgggt ctatccgata aagagctatc tggagaaggc gtaaccggac ggggattctt
     1681 ttttcccgta gctccctatt aaaattgttt tctcatcagt tctttcgctt cctcgaaata
     1741 ctgaagggcc gactccgagg tcctgagcct caggagacgc tgtacctccg acacggtggc
     1801 cccttcctct attgccagca tagcggcagt gtgtctcagg ctcgcgggcg ttatcccact
     1861 tttcttgatc cccgagctct cgaaatagtg gtttatgcgc gccctcagtc cgcgcgtcga
     1921 gattctctct tttaccgccc tgttgcctat accccggaat aaaggctctt caggaccgga
     1981 ttctcccctc tcttcgagat acttcatcag ggtttcctcc atcggctgag caaccgacac
     2041 gtactcgtcc ttcctgtcct tacttttacc ctgcacgtaa attaccctct gccccccttt
     2101 gtatttcagg tcccccaggt taagcctgac gatttctatc tcgctcaacc cggctctcac
     2161 catcaggccg agtatagccg cgtctctccg gccgagggga gaggacttat cgactgcgtt
     2221 catgagcttt gagacgtctc tccgtgtgat gggatcggtc aggtgacggc ggggacgggg
     2281 agcgcccttg acctttcttg ccgggttttc ccggactgtg cctgtagaaa cgaggtagtc
     2341 gtaaaacctc ctcacggctg tcaggtagga agacatggac gtggccgaga gccgcttgga
     2401 aataatgtaa tctttgtagc gctgtatatc gtcaggcgtc atcacggagg gcgatgtgcc
     2461 gccgagccat ttcgagaact cgcgaagggc ttttcggtac gtctctttcg tggtctccct
     2521 tctccggagc gagcctataa aagcccttat taattcttcc ataactgaga atttaatcat
     2581 atttaccacc cgtattcaac atcggccctt gacatgctct ctatttgttg taatttattc
     2641 tgcagaatct aggtggcaat tctgaagagg tcacacccgg tcccattccg aacccggaag
     2701 ttaagctctt cgaggccgat gatactgctc ctgtacggga gtgggaaagt aggaagctgc
     2761 cttggttctt tttttttgcc cttttgctcc cccgctccgg aaccatttcc cggtctttgc
     2821 ctcgtatatt acggtaaatt gtttttagca aacctttgtg tactattttc tcgtttcgat
     2881 atcgtggtca aagaggcggc aggatttgaa aaagacaagg ctcgcggtat tcgtttcggg
     2941 cggcggcacg aacctccagg ctattatcga cgcccgcatc ccttccgtgg atatcgttct
     3001 cgttttcagc aacaaccccg gggcttacgc cctcgagcgg gcgtccaatg aggggatacc
     3061 ctgcgaagtg ctcgaccata agaagtacgc gagccgtgat gaatacgaca gtgaagctct
     3121 tgagttaata gagccctacg atatcgacct gatagcgctg gcgggtttta tgaggatact
     3181 ttccccccgg ttcgtcaggg catatcaaaa tagaatcctg aacatccatc ccgccctcct
     3241 tccctcgttc ccgggcgtta acgctgcgcg gcaggcgctt gaggcgggcg tgaagcatac
     3301 gggggtcacg gtccatttcg tcgatgaggg cgtggatacg ggccctataa tactgcagtc
     3361 ggtcgtgccc gtgctcgacg acgataccga ggagagcctt ctcgaaagga tacacagtga
     3421 ggagcacagg atataccccg cagcgattga gctcgtcgcc tcaggtaaat tcaggataga
     3481 gggtaaaagg gtaatagcgc tggaatagtc cccgagggga acaccctata tttcccttcc
     3541 gactgctttc gcgacaggag ccgccttttc catcaacgcc gcgaactttt tgggtttgag
     3601 agactggccg ccgtcgctta tcgcgacctc aggattattg tgaacttcta ttattagtcc
     3661 gtcggccccg gctgcgat
//
LOCUS       DASSJH010000168         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1445254, whole genome shotgun sequence.
ACCESSION   DASSJH010000168 DASSJH010000000
VERSION     DASSJH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445254"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1503)
                     /gene="tilS"
                     /locus_tag="VFJ67_03620"
     CDS             complement(151..1503)
                     /gene="tilS"
                     /locus_tag="VFJ67_03620"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7289503.1"
                     /translation="MKKTIEKYGMLFPGDRVVVGVSGGADSLGLLHALLELEEYRPEL
                     IVAHLNHGIRGKEAQRDASFVKRAAESCGLKFELGKADAPGYKKEKKLSLEKAARILR
                     YEFFEKTRSKFGADKIATAHTLDDQAETVLMRLIRGSGLRGLSGIPPVSRGVIIRPLI
                     ETPRPDIEKYLESKGIAWIEDSTNKLRTMQRNRIRLDLIPELGAYNPRIKETLSRTSD
                     ILRSEEDYIQREAEKNFRRVFIPGGGELRGDLKKFGRLHQALRLNMLRLAVDDLNKSL
                     KNIASLHLLSADEFLMSDSVSGEVEFPDEIVIAKGHDSFLVTTRLGLEHKFSYFIGSP
                     GKWKFPEFGVDIKEVPAKSLEKEREDVAYFDARKVVFPVEVRNFRPGDRFIPLGMKEE
                     KKLKNFFVDEKIPRFERYRTPIFTSRGEIFWIGGMRIDERFKIRKKGTKAIRMSLVAP
                     "
     gene            complement(1487..2155)
                     /locus_tag="VFJ67_03625"
     CDS             complement(1487..2155)
                     /locus_tag="VFJ67_03625"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET7289504.1"
                     /translation="MRHIYSFFKQNILAGVLVTVPFGLTFLILYKLGAWIVSLLSAAP
                     ARLIGGYLAELPRGLYEASLFLIGLTGTLLVVLFIGAVARNFVGRKLVGFGEAIITKI
                     PLSRTIYIATKQVINTLFVGTGIKNLKRVALLEFPRKGVYSIGFITGSLEPGVHQNQS
                     DKKLLSVFVPTTPNPTTGYYVMLPEDEVTELPIAIEEAFKIIMSGGLATNNIENALPG
                     EKND"
     gene            complement(2159..2443)
                     /locus_tag="VFJ67_03630"
     CDS             complement(2159..2443)
                     /locus_tag="VFJ67_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HET7289505.1"
                     /translation="MTKKELADVIHTKIGLSKRESAEIVEFFFEVAKEKLSRGEGIKL
                     PRFGSFRVQSRKARKGRNPATGETIEIAERRAVVFKPSRFLRDAIDKQTA"
     gene            complement(2465..>2955)
                     /locus_tag="VFJ67_03635"
     CDS             complement(2465..>2955)
                     /locus_tag="VFJ67_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7289506.1"
                     /translation="EIGFLHPGKSAVLMAGAGDEDCGFIGELHPDLSDRLGISKRLYI
                     AEFDLDALTRKFRKTRKAFTPLPKFPSVRRDIALIVDEETPAGSILEEIIKLDSKLIE
                     DADVFDVFTGGQVEKGKKSVAVSLHLRAQDKTLTEEEINKVQDKTIKKLQLALGADLR
                     TI"
BASE COUNT          582 a          817 c          768 g          788 t
ORIGIN      
        1 tatcgccgag ttaaagagaa gggcgctcag ggcgcctgcg gaaagggtga aggttatgaa
       61 gagcgttctc ggggacgacg aggggattgt aggcgcttcc tatctggcgc ttcatgccgg
      121 ttaggaaacg tgaggcggta tgccggagat tcagggggcg acgaggctca ttctgatcgc
      181 cttcgtccct ttcttcctaa tcttgaacct ttcatcgatc ctcattcccc cgatccagaa
      241 tatctctccc ctgctcgtga agatcggcgt cctgtatctc tcgaagcgag ggattttttc
      301 gtccacgaag aagtttttca gtttcttctc ctccttcatc ccgagcggga taaacctgtc
      361 gccgggcctg aagtttctga cctccaccgg aaaaacgact ttccgcgcgt cgaagtatgc
      421 gacgtcctct ctctcttttt cgagagactt tgccggcacc tccttgatat cgaccccgaa
      481 ttccgggaac ttccattttc cgggagagcc gataaagtaa gagaatttat gctcgagccc
      541 taacctggtt gttacgagga aggaatcgtg cccctttgcg atcactatct cgtccggaaa
      601 ctccacctct cccgagaccg agtcggacat gaggaactcg tcggccgata ggaggtgaag
      661 tgacgcgata ttcttaaggc ttttattgag gtcatcgact gcgagtctca acatattcaa
      721 tctgagcgcc tggtggagcc gcccgaattt cttcaagtct cctctcaatt cgcctccgcc
      781 cggtataaaa acgcgcctga agttcttttc ggcttcacgc tgaatatagt cttcctcgct
      841 cctgagtatg tcggaagtcc tcgaaagggt ctcctttatc cgggggttat aggctccgag
      901 ctcgggaatc agatcgagcc ttatcctgtt tctctgcatg gtcctgagct tgttcgtcga
      961 atcttcgatc cacgcgatac ccttcgattc gaggtacttt tctatatccg ggcgaggggt
     1021 ctcgatgagg ggccttatta tcacgcccct agagacaggc gggattcccg aaagtcctct
     1081 caagccgctt cccctgatga gtctcatgag cacagtttca gcctggtcgt cgagcgtatg
     1141 ggcggtagcg attttatccg ctccgaattt gctgcgcgtt ttctcaaaga attcgtaacg
     1201 gagtatacgc gccgcctttt cgagagatag ttttttttct tttttatagc ccggcgcgtc
     1261 tgccttgccg agctcgaact taaggccgca ggactcggcc gcgcgcttta cgaacgatgc
     1321 gtccctttga gcctcttttc ccctgatgcc gtgattcaga tgcgcaacga tcagctccgg
     1381 tctgtattcc tctaactcca ggagggcatg aaggagccct aacgagtccg cgcctccgga
     1441 gacaccgacg actactctgt cgccggggaa gagcatcccg tacttttcaa tcgttttttt
     1501 cacccgggag agcattttca atgttgttgg tcgcgaggcc acccgacatt attatcttga
     1561 acgcctcctc tatcgctatc ggaagctcgg ttacttcatc ttcggggagc attacataat
     1621 acccggtcgt gggattcggg gttgtcggga cgaatacgct gaggagcttt ttgtcggatt
     1681 ggttctggtg gacgcccggc tcgagcgatc ccgttataaa gcctatcgag taaacacctt
     1741 ttctcggaaa ctccaggagg gcgaccctct tcagattctt gatcccggtt ccgacgaaga
     1801 gcgtatttat tacttgcttc gtcgctatgt agatcgttct ggataacggt atcttggtga
     1861 ttatcgcctc gccgaagccg acgagcttcc tgccgacgaa gtttctcgcg accgctccga
     1921 taaataagac gacaagcaat gtgcccgtga gtcctatgag aaagagtgag gcctcatata
     1981 aacctctcgg gagttcggcg agatagccgc cgatgagcct tgcgggagcc gcgctcagga
     2041 gggatacgat ccacgctccg agcttgtaca atatcaaaaa tgtgaggccg aacggtaccg
     2101 tgaccagtac gccggcgagt atgttctgct tgaagaagct atagatatgc ctcatgcatc
     2161 aagctgtctg cttgtcgatg gcgtcgcgga gaaacctgct gggtttgaat acgacagcgc
     2221 gtctctcggc gatctcaatc gtttcgccgg tggcggggtt ccttcctttc ctggccttcc
     2281 tgctctgtac gcggaagctg ccgaaccggg gaagctttat tccctcgccc ctgctgagct
     2341 tttctttcgc tacctcgaag aaaaactcga ctatttccgc agactccctc ttggagagcc
     2401 cgatcttggt atggataaca tccgcgagtt cttttttcgt catgtcgctt acctctcctg
     2461 tttattatat tgtacgtaaa tcggccccca gagccaattg gagcttttta atcgtcttgt
     2521 cctgaacttt gtttatttct tcctcggtga gggtcttgtc ctgagccctg agatggagcg
     2581 aaaccgcaac actcttcttg cccttctcga cctggccccc ggtgaatacg tcgaacacat
     2641 cggcgtcctc tatgagcttc gaatcgagct tgataatctc ttcgagtatg ctgcccgcgg
     2701 gtgtttcttc atccacgatc agcgctatgt cccttcttac agaggggaat ttgggaagcg
     2761 gggtgaacgc cttccgggtt ttccggaact tacgggtcag agcgtcgagg tcgaactccg
     2821 caatgtagag cctcttcgag attccgagcc tgtcgctgag gtcagggtgc agctcgccta
     2881 taaagccgca gtcctcgtcc ccggccccgg ccattagaac ggccgatttg cccgggtgga
     2941 ggaatccgat ttccg
//
LOCUS       DASSJH010000169         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1009785, whole genome shotgun sequence.
ACCESSION   DASSJH010000169 DASSJH010000000
VERSION     DASSJH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009785"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2074
                     /gene="uvrA"
                     /locus_tag="VFJ67_03640"
     CDS             <1..>2074
                     /gene="uvrA"
                     /locus_tag="VFJ67_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7289507.1"
                     /translation="GKSSLAFDTLFAEGQRRYMESLSAYARQFVEQIDKPDVDSIEGL
                     SPSISVDQKTFQRNPRSTVGTITEIYDFLRLLFARLGKPYCSSCGTEISAQSVDRMTE
                     RVAGLGEGEKVSVFSPIVQGRKGEYKKELEDLRREGFLKVRIDGKLIDLDEEIKLNKQ
                     KKHTIELLVDVIVIRPGVRDRINESLKLALKRSGGVAKAETASGALLSLSDKFACPEC
                     GMSYPEISPRLFSFNSPYGSCPTCQGLGETWFCDPELFIDEELSIDEGAIIPWRNSNY
                     FNQLTDSVAEYYGIDTSIPFGKLPAKQKKTLIHGSGGEEITLKRERRGRTLRYKDTYT
                     GVAGIITEWYKETDSPPVREKLAQFMRTAECPDCGGARLRKEALSILFNEKSIYDVVT
                     MSSENCLAFFSNLNLTKRESEIGDRIIKEIDSRLTFLTDVGLGYLTLDRSAPTLSGGE
                     AQRIRLATQVGSKLTGITYVLDEPTIGLHPRDNGRLIGTLKSLRDSGNTIIVVEHDEE
                     TIRNADFIVDIGPGAGEKGGKIVAEGNVDELISHRESLTGSYLSGRTCIPVPEARRKP
                     RGFITVEGASEHNLKYIDVSFPLGVFTCVTGVSGSGKSTLVIDTLYNALGRKLYRSKE
                     NVGKYERFAGVGEIDKVVNVEQTPIGRTPRSNPATYTGIFAPIRDLYSMLPQANIRGY
                     KPGRFSFNV"
BASE COUNT          514 a          560 c          617 g          383 t
ORIGIN      
        1 gggcaaatcc tctctcgcct tcgataccct cttcgccgag gggcagagga ggtatatgga
       61 gtcgctctcg gcctatgccc ggcagttcgt ggagcagata gacaagcccg acgtcgattc
      121 catagaaggt ctctctccat cgatctctgt cgaccagaag acgttccaga ggaacccccg
      181 ctcgacggtc gggaccatta ctgaaatata cgatttcctg aggctcctct tcgcccgcct
      241 cggcaagccc tactgctcca gctgcggcac ggagatttcc gcgcagagcg tggacaggat
      301 gacggagcgc gtagcgggac tgggagaggg agagaaggtc tccgtattct ccccgatagt
      361 acaggggagg aaaggggaat acaaaaaaga gctcgaagat ttgaggaggg agggcttcct
      421 caaggtcagg atagacggga agctcatcga cctcgacgag gagataaagc ttaacaagca
      481 gaaaaaacac acgatcgagc tgctggttga tgtcatagta atccgccccg gcgttcggga
      541 caggataaac gagtcgctga aactggcgct taagcgttcg ggcggggttg cgaaggcgga
      601 gaccgcctcg ggcgccctac tcagcctgag cgataagttc gcgtgccctg aatgcgggat
      661 gagctacccc gaaatctccc cgcgtctctt ttcattcaac agcccctacg gctcctgccc
      721 gacgtgccag ggtctggggg aaacatggtt ctgcgaccct gagctcttca tcgacgaaga
      781 gctttcgata gacgaagggg cgataattcc gtggcgcaac tcgaactact tcaatcagct
      841 gaccgacagc gtcgccgaat attacggtat cgacacgtct ataccgttcg gaaagctgcc
      901 cgcgaagcag aagaagacac ttattcacgg ctcgggcggc gaagagataa cactcaagag
      961 ggagaggcga gggcgcacac tcaggtataa ggacacatat acgggcgtcg caggcattat
     1021 caccgaatgg tataaggaaa cggattctcc tccggtgcgg gaaaagctcg cccagttcat
     1081 gcggacggct gagtgcccgg attgcggggg cgcgaggctc agaaaagagg ccctgtcgat
     1141 tctgtttaac gaaaagtcca tatacgacgt cgtaacgatg tcttccgaaa actgtctcgc
     1201 cttcttttca aatctcaatc tcacgaagag agaaagtgag atcggggacc gcatcataaa
     1261 ggaaatagac tcacggctca cgttcttgac tgatgtcggg ctcgggtacc tgacgctcga
     1321 ccgctccgcc cccacgctct caggcggcga ggcgcagagg ataaggctcg ccacacaggt
     1381 gggctcgaag ctcacgggaa taacgtacgt tctcgacgag ccgacgatag gtctccaccc
     1441 gcgtgacaac ggaaggctca tcggcacgct caaatcgctc cgggacagcg gcaatacgat
     1501 aatagtcgtc gagcacgacg aggagaccat aaggaacgcc gattttatcg tcgatatagg
     1561 gccgggggca ggtgagaagg gcgggaagat agtcgccgaa ggtaacgtgg acgagcttat
     1621 aagccaccgt gagtctctca ccgggagcta tctttccgga aggacctgta tacctgtgcc
     1681 ggaagcacgg aggaagcccc gcggttttat caccgttgaa ggggcgtccg agcataatct
     1741 gaaatatatc gacgtatctt tcccactcgg agtattcacc tgcgtcacgg gcgtgtcggg
     1801 ctcgggcaag agcactctcg tcatagacac tctatacaac gccctcggac ggaagctcta
     1861 caggagcaag gagaatgtag ggaagtacga aaggtttgcc ggggtcggcg agatagacaa
     1921 ggtcgtgaac gtggagcaga cgcctatagg caggactccg aggtcaaacc ctgcgaccta
     1981 caccggaata ttcgccccga taagggacct ctactcgatg ctcccgcagg cgaacatcag
     2041 gggatacaag cccggcaggt tcagcttcaa cgtg
//
LOCUS       DASSJH010000170         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_688927, whole genome shotgun sequence.
ACCESSION   DASSJH010000170 DASSJH010000000
VERSION     DASSJH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688927"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /gene="htpG"
                     /locus_tag="VFJ67_03645"
     CDS             complement(<1..940)
                     /gene="htpG"
                     /locus_tag="VFJ67_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET7289508.1"
                     /translation="MAQHTTKKHEFQAEVKQVLDIVIHSLYTDKEVFIRELVSNSSDA
                     LEKLRYIQLTEKDIYDENLPLEINIRTDSEENSITIEDHGVGMSEDELIENLGTIAHS
                     SSKAFLKAVAESGGVSEKLIGQFGVGFYSVFMVADMVEVYTHSREKEGKSLLWKSDGG
                     GEYEIEPAEGVRRGTKIVVRLKEDAKEFSDPERVKDVLTHYSGFVQFPIKLNGEQINK
                     IRPIWMRNKGEVSDEEYSEFYKFQADAFDGPLYRLHFSSEAPLEINALVFVPAENPER
                     WGFGKSDPGVSLYCKKILIDTKPEGLLPDWLRFLKGV"
     gene            complement(1116..1853)
                     /locus_tag="VFJ67_03650"
     CDS             complement(1116..1853)
                     /locus_tag="VFJ67_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7289509.1"
                     /translation="MSNDSFLSRRSFIRIGGAAVLGAELLRAGGSSFYIPEARAADVT
                     SPMTSDEALKALMDGNKRFINSMNESCMQSTLEHRMKVAEGQNPFAVILACADSRVPP
                     EIIFDQWIGDLFVVRVAGNIITPSNYGILGSIEYGVKALGVNLIMVLGHSRCGAVDSA
                     IKAIKDGTKFPGSIETIVQAIKPSVERAKGEPGDLLHNAILDNVRLGVSRISGSDPVV
                     APMVKEGKVKVVGGNYDLVTGEVTLVT"
     gene            1969..>2873
                     /locus_tag="VFJ67_03655"
     CDS             1969..>2873
                     /locus_tag="VFJ67_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET7289510.1"
                     /translation="MKKTKNISQFVCQNCGNHSPRWLGKCPECGSWNSYVEEKLERQK
                     KSAKRADVSEPVAITEIGGGGEERIPTGNKELDRVLGGGFVPGSVVLVGGDPGIGKST
                     LALQMLNDIGREKQKRGPSVSRFLYVTGEESPMQVKMRAARTGVSSESVFVLTETSVE
                     TILEEIEKLSPEIAVIDSIQTMFAEDFGSAPGSVGQIRECTAKLMQYAKTRGTAIFLI
                     GHVTKEGTIAGPKVLEHLVDTVLYFEGGKDHPYRILRAVKNRFGSTNEIGVFEMLDSG
                     LREVKNPSEIFLSERPVNASGSVVTP"
BASE COUNT          642 a          798 c          750 g          683 t
ORIGIN      
        1 cgaccccttt caggaacctg agccagtctg gaagcaagcc ctcgggcttc gtatcgatca
       61 gtatcttttt gcagtaaaga ctcactccgg gatcagattt tccgaagccc catctctccg
      121 ggttttcggc cgggacgaat acgagagcgt ttatctctag gggtgcttcg gagctgaagt
      181 ggagtctgta tagcgggccg tcaaacgcgt ccgcctggaa tttatagaac tcgctatatt
      241 cctcgtccga tacctctccc ttattcctca tccatatagg ccggatcttg ttgatctgct
      301 ctccgttcag ctttatcggg aactgaacga agcccgaata atgcgtgagt acatccttca
      361 cgcgttcggg gtccgaaaac tccttcgcgt cctccttgag acggacgacg atcttcgtcc
      421 cgcgccttac gccctcggca ggctcgatct catattctcc tccgccgtcg ctcttccaga
      481 ggaggctctt tccttccttc tcccggctgt gtgtatagac ctccaccatg tccgcaacca
      541 tgaacacgct gtaaaaaccg acaccgaact ggcctatcag cttctcgctc acgccccccg
      601 attcggcgac cgctttgagg aacgcttttg aacttgaatg ggctatagtg ccgaggttct
      661 caatgagctc gtcctccgac atacctacgc cgtggtcctc gatcgtaatc gagttctcct
      721 ccgaatccgt cctgatattt atttcaagcg ggaggttctc gtcgtatatg tctttttcgg
      781 tcagctggat gtacctgagt ttttcaaggg cgtctgagga gtttgaaacg agctctcgta
      841 tgaaaacctc tttgtccgta taaagcgagt ggataacgat atccaggacc tgcttcacct
      901 cggcctggaa ttcgtgtttt ttcgtagtat gttgggccat ttcgaataac aaacctccgg
      961 attattttcg gtaataaatc agtcccaaac ggctattcta ccgtatgcaa aatattcatc
     1021 gggaggaaaa tttcaagagt ccgagcagtc ttcccgcatt gacagcgggc ttaattcccg
     1081 tctccagcgt atagacagca gggaagacga aaggtctatg tcactaatgt aacttcgccg
     1141 gtcacgagat cgtagttccc gcctacgacc ttgaccttcc cttccttgac cattggcgcg
     1201 acaaccggat cggatccgct tatcctgctc actccgagcc tgacattgtc gaggatggca
     1261 ttatggagca ggtctccggg ctcacctttc gccctttcaa cagagggctt gatggcttga
     1321 actatggtct ctatagagcc aggaaattta gttccgtctt taatagcctt gatggccgaa
     1381 tccactgccc cgcacctgct gtgcccgagg accatgatga ggttcacgcc gagggctttc
     1441 acaccgtact caatgctccc gaggataccg tagtttgaag gagtgattat attccccgca
     1501 accctgacca cgaacaggtc ccctatccac tggtcgaata taatctcggg gggaacacgg
     1561 gagtccgcgc acgcgagaat aactgcgaag gggttttgcc cctctgcaac cttcatccgg
     1621 tgctcaagcg tactttgcat gcagctctca ttcatagagt ttataaaccg tttattcccg
     1681 tccattagcg ccttgagcgc ttcatcgctt gtcattggcg atgtgacgtc ggccgccctc
     1741 gcttcaggta tatagaacga gctccctccc gctctgagca gctcggctcc cagcaccgca
     1801 gctccgccta tgcggatgaa gctacgcctc gataaaaagg aatcgttact catatcttgc
     1861 cctccttgcc tctttattgt atttacagaa atcagccgtt atcgtcaatt ctaatgcata
     1921 tttataataa tgtataatgt catcccggct gttcatccgg acaacgtaat gaaaaagaca
     1981 aaaaacatct ctcagttcgt ctgccagaac tgcggaaacc actcccccag gtggctcggc
     2041 aaatgccccg aatgcgggag ctggaactcg tacgtcgagg agaagctgga aaggcagaaa
     2101 aagtcagcga aaagggccga cgtgtcggag cccgtcgcta taaccgagat cggcggcggc
     2161 ggcgaggaga ggataccgac cggcaacaag gaactcgaca gggtgctggg aggcgggttc
     2221 gtcccgggct cagtcgtact cgtcggggga gacccgggga ttggtaaatc cacgctcgcg
     2281 cttcagatgt tgaacgatat cggcagggag aagcaaaaac gcggcccgtc ggtatcgagg
     2341 ttcctttacg tgaccggcga ggaatcgccg atgcaggtca agatgagggc ggcgcggact
     2401 ggtgtatcgt cagaaagcgt gttcgtgctc actgagacct cggtcgagac gatactcgag
     2461 gagatagaga agctctcgcc cgagatcgcc gtgatagatt ccattcagac tatgtttgcg
     2521 gaggacttcg gctccgctcc cggcagcgtc gggcagataa gggaatgcac cgccaagctc
     2581 atgcagtacg cgaagacgag agggacggcg attttcctca tcgggcacgt aacgaaggaa
     2641 ggcacgattg cggggcccaa ggtgctggag cacctcgtcg atacagtgct ctatttcgaa
     2701 ggcgggaaag accatccgta caggattctt agggcggtca agaacaggtt cggctctact
     2761 aacgagatcg gggtcttcga gatgcttgac agcgggctga gggaggtaaa gaacccctcc
     2821 gagatattcc tctccgagcg ccccgtgaac gcatcgggct cggtcgtgac acc
//
LOCUS       DASSJH010000171         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_39522, whole genome shotgun sequence.
ACCESSION   DASSJH010000171 DASSJH010000000
VERSION     DASSJH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39522"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..1307)
                     /locus_tag="VFJ67_03660"
     CDS             complement(423..1307)
                     /locus_tag="VFJ67_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7289511.1"
                     /translation="MKIHTIDHEFFAKRVIASYLIENGGEPILIESGPDTTFHKLEKG
                     LNEHGYSIEDIRNVFVTHIHLDHSGAAWRFADAGAKIYVHPFGAQHLADPAKLVASAK
                     RIYNEQMETLWGEIRPIARDNIHEIRDGEKIKIGSISIKAVETLGHASHHHSYLVDDL
                     LFTGDAAGVRIDNGPVLPPTPPPDINIDLWRESIRKISRLRPEALYLTHFGKSGDVGD
                     HLKELENRLLEVTAWAGERLKQGKTEEEMIIEMEGMFGEILVKAGADKQLVEAYELAD
                     PFWMNVGGLVRYWKKFRT"
     gene            1432..2268
                     /locus_tag="VFJ67_03665"
     CDS             1432..2268
                     /locus_tag="VFJ67_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7289512.1"
                     /translation="MNILEFSSLVFLGSLLAGFLGAATGLGGGVVIVPLLALGFGVDI
                     HYAMGASLVSVIATSSAAASAYVKEGFSNIRVGMFLEIATTIGAVAGAAIATSISPSD
                     IAVVFGLVLLYSAYQSGFTRHKDAAPCEPDKLATRLRMNSEYPTPEGPKSYYVRAVPQ
                     GFGIMFLAGALSGLLGIGSGALKVIAMDQVMRLPFKVSATTSNFMIGVTAAASAGVFL
                     NRGYVDPGLAMPVMLGVLIGSLAGAHFLFGASTKLLRIVFAAVIFALAAEMLYKGLTG
                     GL"
     gene            2270..2659
                     /locus_tag="VFJ67_03670"
     CDS             2270..2659
                     /locus_tag="VFJ67_03670"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HET7289513.1"
                     /translation="MKEKGGRMSDERIDLIIGNLLRIAVIVSAIFVFAGAVIYLWRHG
                     LEMPDFGVLSGVPKNLRGLKEIIDSAWQIRSVGIIQLGLLLLIFTPVARVAFSVIAFL
                     LERDYMYVVFTLIVLTVLLLSVTGVIV"
     gene            2836..>3026
                     /locus_tag="VFJ67_03675"
     CDS             2836..>3026
                     /locus_tag="VFJ67_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289514.1"
                     /translation="MEINNRQCFRKVLFLSSVLFVLLFFLSGFCGLAVTASAKESAPA
                     EIKEIAKGAYIYAFPMIMN"
BASE COUNT          604 a          788 c          833 g          801 t
ORIGIN      
        1 ggcgcacagg ggctatgtcg accactgtga ggctcgcaat cgacgcatcg tcggtggccg
       61 atacagccat agctgcaacc ccgcccatgg aatgtcccac aaggtgagtg ggctcgacgc
      121 agttcgtctc gatgagatgc acgatgtcat cccgcatcgc ctcgatgctg tgagggccgt
      181 gcggcgaatc gccgtgattg cgcaggtcag ggacgatcac tcggcagtcg gctgacagcc
      241 cgcgcataat gcgccgccag ttccgtgaag accccaggag cccgtggagg ataacgactt
      301 cggagccgga gcttccggat ttaatgaggt tgagttccat cttacttttt cttccgtcta
      361 gatctgtctt tgtgtgctcg caagtttaga gctgcctcac agccggatcc atccgtacgg
      421 gctcacgttc ggaacttttt ccagtagcgc acaagtccgc cgacattcat ccagaacggg
      481 tctgcaagct catatgcttc gactaattgc ttatccgctc cggccttgac gagtatctcc
      541 ccgaacatac cttccatttc aattatcatt tcctcttcgg tctttccctg tttcagtctc
      601 tctcccgccc atgctgtgac ttcgagaagc ctgttttcga gctccttcag gtggtctccg
      661 acatcgcccg acttgccgaa atgagtgagg tacagggctt ccggcctcaa tctcgagatc
      721 ttccgtatcg attcccgcca gagatcgatg ttgatatcgg gtggcggggt agggggcagt
      781 accggcccgt tatctatgcg tacgccggcc gcgtctcccg taaacaggag atcgtccacg
      841 agataggaat gatggtggga cgcgtgcccg agggtttcaa cggccttgat actgatgctc
      901 ccgattttta tcttttcccc gtcccggatt tcgtgaatat tatccctcgc aatgggccgg
      961 atttcgcccc atagcgtttc catttgttcg ttataaatac gttttgcgga tgcaacgagc
     1021 tttgcggggt cggccagatg ctgggcaccg aatgggtgaa catagatttt cgcgcccgcg
     1081 tcggcgaagc gccatgccgc cccggaatgg tcaaggtgta tgtgtgtcac aaaaacgttt
     1141 ctaatgtctt ctatagagta accgtgctcg ttcaggccct tttccagttt atggaaagtc
     1201 gtatcggggc ctgattctat taatatgggt tcgccgccgt tctcgataag atacgaagca
     1261 atcaccctct ttgcaaagaa ctcatggtct attgtgtgaa tcttcatcaa aaactcctct
     1321 tacttttaat gctgcgtccg tttccgcaaa tgctccggtc tgttatgatg tagatacgta
     1381 caagagaata agttattctc gaatcgcatg atgagctgaa aacccggtca aatgaatata
     1441 cttgaattct cctcgctcgt ctttctcggt tctctccttg cgggtttcct cggcgccgcc
     1501 acgggcctgg gcggcggtgt ggtgatagtg cctctcctcg cgctcggctt cggcgtggat
     1561 atacattacg cgatgggcgc ttccctcgtc tcggtgatag caacgtcatc ggctgctgcg
     1621 tccgcatacg tcaaggaggg gttcagcaac ataagggtgg ggatgttcct tgagatcgct
     1681 actacgatcg gcgcggtcgc gggagccgcg atagccacga gcatctctcc ctccgatatt
     1741 gccgtggtct tcggactcgt gcttctatat tcggcctacc agtccggttt cacccgtcac
     1801 aaagacgcag cgccctgtga gcctgataag ctcgccacgc gtctcaggat gaattccgaa
     1861 tacccgacac cggagggtcc taagagctac tatgtccgcg cggtccccca gggtttcggg
     1921 ataatgtttc tggcaggcgc cctttcgggc ctcctgggaa taggttcggg ggccctcaag
     1981 gtgatagcga tggaccaggt aatgcgtctc cctttcaagg tatccgcaac gacgagcaac
     2041 ttcatgatag gcgtgacggc cgccgcgagc gcgggggttt ttctaaaccg ggggtacgtc
     2101 gatccgggtc ttgccatgcc cgtcatgctg ggggtgctca taggctctct cgcaggagct
     2161 cattttctgt tcggcgcatc gacgaagctg ctgcgcatcg tgttcgcggc agtcatcttc
     2221 gctctcgcgg cagagatgtt gtataagggt ctcacgggag gtctttgata tgaaagaaaa
     2281 ggggggcaga atgagcgatg agcgtatcga tcttataata ggaaaccttc taaggatcgc
     2341 ggtaatagtt tcggcgatat tcgtgtttgc gggagcggtg atttacctct ggcgtcacgg
     2401 actggaaatg ccggatttcg gggtcttatc gggcgtgcct aagaacctgc gcggcctgaa
     2461 ggagataatt gactctgcat ggcagataag gagtgtgggg attattcaac tgggtcttct
     2521 gctgctcatc ttcacacccg tggcgcgagt cgcattttca gtaatagcct ttctgctcga
     2581 gcgggactac atgtacgtgg ttttcacact gattgtgttg acggtccttc tcctgagcgt
     2641 cacgggagtt atagtctgat ccgggcgctt cggtatcctg agcctcccga tcgaaagagt
     2701 tgtaatgcga tttcggggcg aagttgataa atgccccaac ggctattaga attcgaaatg
     2761 gggatagaat taaggtttgt atttatcgat ctttatagtg cctgcatcta agtgcgtgtt
     2821 agaccaggag gacgtatgga aattaataac aggcaatgtt ttcgaaaagt ccttttttta
     2881 tcgtccgtgt tatttgtgct tttattcttt ttatcgggat tttgcgggct ggccgttaca
     2941 gcttcggcca aagagtccgc gcccgcggaa ataaaggaga tcgctaaggg tgcttacatc
     3001 tacgccttcc ccatgataat gaacta
//
LOCUS       DASSJH010000172         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_364645, whole genome shotgun sequence.
ACCESSION   DASSJH010000172 DASSJH010000000
VERSION     DASSJH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364645"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFJ67_03680"
     CDS             <1..318
                     /locus_tag="VFJ67_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose dehydrogenase"
                     /protein_id="HET7289515.1"
                     /translation="VLGKNGVLVLSSVTGGERKIEVPADMINLGFVLGNKVVVGTVNA
                     NREYFEMGVKDLCHAELEWPGLLAKFLTHPVNGLDNYNELMSKLTEAKGAIKVYCEIS
                     PLN"
     gene            335..1162
                     /locus_tag="VFJ67_03685"
     CDS             335..1162
                     /locus_tag="VFJ67_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET7289516.1"
                     /translation="MPTILKKHWPEYLMEGAGLGFFMISACFFVALLEHPSSPAHAAI
                     PDPMTRRMLMGMAMGLTAIAIIYSPWGKQSGAHINPSVTLTFFRLGKVRTLDAAFYII
                     AQFIGAAAGVLIAHVLLGKIASHPHVNYAATFPGMNGVVIAFLGEAVISFILMTVILN
                     VSNTPAIARYTGIFAGLLVAAYITFEAPISGMSMNPARSLGSSLFASSHAGLWIYFTA
                     PLFGMLLAAEAYVRMKGAAAVSCAKLHHQNDKRCIHCGYPGAVRADNVHAINERVKI"
     gene            1199..1612
                     /locus_tag="VFJ67_03690"
     CDS             1199..1612
                     /locus_tag="VFJ67_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289517.1"
                     /translation="MFKSFESKSFKTAVVSIAMILALAFWAQGGIAGETMTSAPFAGV
                     KVNGGTVTLTKEGDKTILTLSDDFVVPQTPDPHWQVVDSKGNVYLLQRLPIKGDKVNK
                     SITLPPYVPDVAKVQIWCAFAETLLGEAPFASHMN"
     gene            1710..2570
                     /locus_tag="VFJ67_03695"
     CDS             1710..2570
                     /locus_tag="VFJ67_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7289518.1"
                     /translation="MRLNGLIIGLLFIVAAVSAGAQVTEKKSITLEGANIVIAGAMAE
                     AKKLNAPGGVIAVVDDGGNLVALERIDGTFAAGANISIGKAKTAVLFKKPTKFFEDVI
                     KNGRTSMVALPDFTPLQGGVPITVDGEIVGAVGVSGAASAQQDEELAIAGANALMTKK
                     SGEEGTAPVTYFDRDKVSAAFAKGMPLTEVANYKVHASRRTEPGVAEVHTRDTDIIYM
                     LEGSATLVTGGNVVEGKTTAPDEIRGKEISGGETRSLNPGDVIIVPAGVPHWFKDVKG
                     PVLYYVVKVS"
     gene            2588..3085
                     /locus_tag="VFJ67_03700"
     CDS             2588..3085
                     /locus_tag="VFJ67_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7289519.1"
                     /translation="MKLNLISMLLISIISVAHAAAAETPLKPVLTLGAAKKAVEAAEK
                     EAAKRGATVVIAVVDDGGQLILLERLDDTQVASVEVGIGKARTAAIFRRPSKVFEDQI
                     REGRVAALALPGATPLQGGIPIIIDGKVVGAIGVSGNTPQEDEEIAIVGASAIGEVIA
                     SGEKK"
BASE COUNT          748 a          876 c          975 g          620 t
ORIGIN      
        1 gtcctcggga aaaacggcgt cctcgttctc tcgagcgtca cgggagggga gcgcaagata
       61 gaggtccccg ccgacatgat caatctcgga ttcgtgctcg ggaacaaagt agtcgtcggc
      121 acggtcaacg cgaaccgcga atacttcgag atgggcgtta aggacctctg ccacgcggag
      181 ctcgaatggc cggggctcct cgcgaagttc ctcacgcatc ccgtgaatgg gctcgacaac
      241 tataacgagc ttatgtccaa gcttacggaa gcgaagggcg cgataaaagt atattgcgag
      301 atatcgcccc tcaactgaaa aacggaacac gccgatgccg actatattga aaaaacactg
      361 gcccgaatat ctgatggaag gcgcaggcct cgggttcttc atgatatcgg cctgcttttt
      421 cgtcgcgctc ctcgagcatc catcctctcc tgcacacgct gcgatacccg accctatgac
      481 gaggaggatg ctgatgggaa tggcgatggg actcacagcg atagctatca tatactcgcc
      541 gtggggaaag cagtctgggg cgcatattaa cccgtcagta accctcacat tcttcaggct
      601 cggaaaggtc aggaccctgg acgccgcctt ctatattatc gcgcagttca taggagctgc
      661 ggcgggggtg cttatagcgc acgtgctcct tggaaagatc gcgtctcacc cccatgtcaa
      721 ctacgcagcg acgtttcccg gaatgaatgg ggtggttatc gcgtttctgg gggaggcggt
      781 catctcgttc atacttatga ccgtcatcct gaatgtttca aacacgcccg cgatcgcgag
      841 atacacaggt atcttcgccg ggctcctcgt tgcggcttac ataactttcg aagcgcctat
      901 ctcgggcatg agcatgaacc cggcgcgctc tctgggatcg agtctcttcg cgagcagcca
      961 cgccgggctg tggatctact tcacggcgcc acttttcgga atgctgcttg cggccgaggc
     1021 atacgtgcgg atgaagggag cggccgcagt ttcctgcgcg aagcttcacc accagaacga
     1081 caagcgatgc atacactgcg ggtatccggg agcggtcaga gccgataacg tccacgcgat
     1141 caatgaaaga gtaaaaatct gagctttgag aacagttata aaaaaaggag gtattacaat
     1201 gtttaaatca tttgagtcca agagcttcaa aacggcagtc gtatcaatcg ctatgatcct
     1261 ggcgttggct ttctgggcac aaggcggcat cgcaggagaa accatgacgt ccgccccttt
     1321 cgcaggcgtt aaggtaaacg gcgggacggt aacactcaca aaggagggag acaaaactat
     1381 cctcactctg tcggacgatt tcgtcgtccc tcagaccccc gatccgcact ggcaggtagt
     1441 tgattcgaag gggaacgtct atttactcca gcgtctgccc atcaagggcg acaaggtgaa
     1501 caagtcaatc accctgcccc cgtacgtgcc cgacgtggcg aaggtccaga tatggtgcgc
     1561 tttcgctgaa acattgctgg gagaagctcc tttcgcttcg catatgaact agatctgcgg
     1621 ttggacctgc gcctgacgga ggtgtcccga ccgcgggacg cctccggcct gtcggaggga
     1681 aagtggaaat ctacagggag gtatcaaata tgagattgaa cggactgatt atcggattac
     1741 tttttatcgt ggcggcggta tcggccggcg ctcaggtcac ggagaagaaa tcgatcacgc
     1801 tcgagggagc taatattgtt atcgccggcg ctatggccga ggccaaaaag ctgaacgccc
     1861 caggcggtgt gatagcggtc gtggacgacg gggggaacct cgtcgcactc gagcgcatag
     1921 acggaacttt cgcagccggg gcgaatatct cgataggcaa ggcgaagact gcagtgctct
     1981 tcaaaaagcc gaccaagttt tttgaggacg ttataaagaa cgggcgcacc agcatggtcg
     2041 cccttcccga cttcacgccg ctccagggcg gggttcccat caccgtcgac ggcgagatag
     2101 tcggagccgt cggggtgagc ggcgccgcga gcgcccagca ggacgaagag ctcgcgatcg
     2161 cgggagcgaa cgctctcatg acgaaaaagt ccggagagga gggcaccgct ccggtcactt
     2221 acttcgaccg cgacaaggtc tcagccgcat tcgccaaggg gatgcccctg acggaggtcg
     2281 cgaactataa agtacacgcg agccgcagga ccgagcccgg cgtcgccgaa gtgcacacca
     2341 gggacaccga catcatctat atgctcgaag ggtcggcgac gctcgtcacg ggcggcaatg
     2401 tcgtcgaagg taaaacgaca gcgcctgacg agatacgcgg gaaggagatt tccggcggcg
     2461 agacgcggag cctcaatccg ggcgacgtga taatcgttcc cgcaggagtc ccccactggt
     2521 ttaaagacgt gaagggacca gtgctctatt acgttgtgaa ggtcagttag accggggagg
     2581 tatgaaaatg aaattaaatc ttatctctat gctcttaatt tcgattatat cagtcgctca
     2641 cgcggctgca gccgaaaccc ctcttaaacc cgtgctcacg ctcggcgccg cgaagaaggc
     2701 ggtagaggcg gccgagaaag aggcggcaaa gaggggagcg acggtagtga tcgccgtggt
     2761 cgacgacggt gggcagctga ttctgctcga gcgtctcgac gacacgcagg tcgcgagcgt
     2821 cgaagtggga ataggcaagg ccaggactgc tgccatattc aggaggccga gcaaggtgtt
     2881 cgaggaccag attcgggagg ggcgcgttgc agccctcgcg cttccggggg cgacaccgct
     2941 ccaggggggc atccccatta tcatagacgg caaggtcgtg ggagcgatcg gcgtgagcgg
     3001 gaacacccct caggaagacg aagagatcgc gatcgtggga gcttccgcaa tcggcgaggt
     3061 catagcctcg ggagagaaaa aatgagactc gaatcgatca tatatacggc cgcttttctg
     3121 ttgtcgatca ttgccgcgct ggctgtgcac gcagcatcgc ccgttgatgt gccgagcgga
     3181 aaaccttcag ccgtcatcga cctggctacg actaagggc
//
LOCUS       DASSJH010000173         3686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1552756, whole genome shotgun sequence.
ACCESSION   DASSJH010000173 DASSJH010000000
VERSION     DASSJH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552756"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            541..1005
                     /locus_tag="VFJ67_03705"
     CDS             541..1005
                     /locus_tag="VFJ67_03705"
                     /inference="COORDINATES: protein motif:HMM:NF037752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289520.1"
                     /translation="MKGFNLFMLLAVLFLSDFALAQIPSCPCDTTELPSGVSGIEIIE
                     TLCPGGELADDAGSNVTQETVQIFTLEPGGASYGTFTDGSNLGCSISDGNIALLRSVT
                     EQEFEDCNERLIEGCDLLTRSIPTLSQWSMMATAGVLGLIGLIYTLRRKTAA"
     gene            1357..1668
                     /gene="rplU"
                     /locus_tag="VFJ67_03710"
     CDS             1357..1668
                     /gene="rplU"
                     /locus_tag="VFJ67_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019218292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET7289521.1"
                     /translation="MQAVIKTGGKQYSVNKGDVIDVELLTGNPGDVVEFKEVLMVADE
                     GGTTVGSPVVGNAIVKAKILGTKKGEKLIVFKFRRRKDFRKKTGHRQKYTSVEITEIS
                     R"
     gene            1684..1944
                     /gene="rpmA"
                     /locus_tag="VFJ67_03715"
     CDS             1684..1944
                     /gene="rpmA"
                     /locus_tag="VFJ67_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012172613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET7289522.1"
                     /translation="MATKKGGGSSKNGRDSRGRRLGLKKFGGETVKAGNILVRQRGTK
                     FHPGLYVGMGRDHTLFALKDGVVEFQTYKKTKTRINIQPEQQ"
     gene            2248..3483
                     /locus_tag="VFJ67_03720"
     CDS             2248..3483
                     /locus_tag="VFJ67_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289523.1"
                     /translation="MGLKSRVGNIYSGNALVLAFIFVFISLLISLFNSYYYTSTITDE
                     RGQEMARLFDGTGKSFADTSKEILNTRHDIVYIKLLNPDGVLLESFGGDDTGDLEIIP
                     INTPDNNTIVLGVVGKDWKSLALSSLMWSALIGIAVSVVIIFLLSLFSSGRNDSMENL
                     IAAMKSVSRGDLAGARLDPESSDDVSTIRAYETFNQMVDRMKKRESIEEDLPPFQPTL
                     ISADDEDEEEETISRPRNVTVVVAKIADFQELSNTLKPAEFNAFLADYRKAASAIISN
                     YGGVIEALLKDEIVAFFNAPEEQVNPELKAVCSAVEVLQLLASITRERKIEGKAVISG
                     KIGIGMKSVPVYDDSSMPHGVKQVTDMARRICTVAPVWRVIVSADVYQIVKDFVEARE
                     LKLGNEPYYSIVGVEEGVV"
BASE COUNT          987 a          934 c          987 g          778 t
ORIGIN      
        1 ttcgcagaac ctaccagcct gccgtcgccg aggaagacca gtcctgttac gttgtcgaaa
       61 ccgatagggc cgatgagggt cgcgttgccg ttgctggggt cgagcaaata aagactgctc
      121 gacagggggg cattctcccc actcggggat ccggttgcgg caccgtataa taactgcgca
      181 tttgccgacg ccgtaagagc cgcaattaac aatagtgtat aaaaacaggc cttgattgtt
      241 tccacaggct aatcctcccc cccaaacgac tgttatttaa taatcagtat taatgcgaat
      301 attagcatta atgataaaaa attaccagat ttttaagatg cgaatcaggc gtgtatatga
      361 ttggcgcacg ggacagacgg gacgcatcat ccgggtatat accccgctta tcggtcccat
      421 aaccggaact gtctttgaac caaaaaatcg agtgaaaccg tctgcttgct taacgttcct
      481 gacatatgtt aatatcaaca tattcacctg ttattgatct aatggaggag gtggcggatc
      541 atgaaaggct ttaacctgtt tatgttattg gcagtcttgt ttttatccga tttcgctttg
      601 gctcaaatac cttcatgccc gtgcgatacc acagagctgc cgagcggtgt gtctgggatc
      661 gaaataatcg agaccctctg cccgggcgga gagcttgccg acgacgcagg ttcaaatgtc
      721 acgcaggaga ctgttcaaat ctttacgctg gaaccgggag gtgcgagcta cggcactttc
      781 acggacggct cgaatctagg gtgcagcata tccgatggaa acatagccct gctgcgaagc
      841 gttaccgaac aggaattcga ggattgcaat gagcgcctca tagagggctg cgacctactg
      901 accagatcca tcccgaccct gtcccaatgg agcatgatgg cgacggcagg ggtgctgggg
      961 ttgatcggat tgatttatac gctgagaaga aaaactgcgg cgtaaacgag gcgaataaat
     1021 aaaaagaatt aagaagacgt ggacccgtcc atggctaagt cctatggccg ggcattgtcg
     1081 ccggggaggc tgaacgaatc gtcacccgcc ccctcaaaat atgaagcccc ccttcaaata
     1141 ttgaagccca gggggttaaa atttgacccc taaggaatac acatttaagt aatacacaat
     1201 taatgaatac acagtaaaaa aatgcgcgta aaaaaatacg cgcgcctgaa gaagctcacg
     1261 cttcctggaa ttctgcttct ctaatattca aaaatttgac aacacgccat aaatatgaat
     1321 aattttaccc tttcacagaa gcgggaggag tatatcatgc aggctgtaat caaaaccggc
     1381 ggaaagcagt acagcgtgaa caaaggcgac gtcatagacg ttgaattgct gaccgggaat
     1441 ccgggcgacg tagtggaatt caaggaagtg ctgatggttg cggacgaagg cggtaccaca
     1501 gtaggctcgc cggtcgtcgg taacgcaatc gtgaaggcga agatactcgg cacgaagaaa
     1561 ggcgagaagc tcatcgtctt caagttcaga aggagaaagg acttcagaaa gaagaccggg
     1621 caccgtcaga agtacacctc ggtcgagatc accgagattt caaggtaggg aggcgtataa
     1681 caaatggcaa ctaaaaaagg cggcggcagc tctaaaaacg gaagggactc cagggggaga
     1741 cggctcgggc tcaagaagtt cggcggcgag acggtgaagg cggggaacat actcgtaagg
     1801 cagaggggaa cgaagttcca tcccggcctc tacgtcggca tgggacgcga ccataccctt
     1861 ttcgcgctca aggacggggt cgtcgagttc cagacgtaca agaaaaccaa gaccagaatc
     1921 aatatccagc ccgagcagca gtaggctcaa tatccccggc cggggtttca ggcgggacgg
     1981 catagccgca cgcgcatttc ggctcccgcg gggattagac ccggcattcg gccggaatct
     2041 attatttcgg atcaatcggt tacactcaaa gggccgtcaa gaaaaagacg ccgggaattt
     2101 tcttaacact ctgttaaaat taatctttac cagttaaatt ttagccagta ttatgaagtt
     2161 agtttatctg ttccaatctt caaaagagac ggatgcgttc ccgacccgcg aaacgcattt
     2221 gatcggacag ccggagaaac gaggcaaatg ggattaaagt ccagggtagg gaatatatat
     2281 tcgggaaacg cgctcgtact cgccttcata ttcgtcttca tttccttatt gatttcgctc
     2341 ttcaactcct actattacac ctcgaccatt actgacgaaa gaggtcagga gatggcccgt
     2401 cttttcgacg gcaccggcaa gagcttcgcc gacacctcga aggaaatact caatacgaga
     2461 cacgatatcg tgtacataaa gctcctcaac ccggatggcg tgctcctcga aagcttcggg
     2521 ggggatgaca ccggagacct cgagataatc cctattaaca cccccgataa caacaccata
     2581 gtgctgggcg tggtcgggaa ggactggaag agcctcgctc tgtcctcgct catgtggagc
     2641 gccctcatag gcatagccgt ttcggtggtg attatattcc tgttgtccct cttctcttcc
     2701 ggacggaacg actcgatgga aaacctgata gcggcgatga agagcgtctc ccgcggagac
     2761 ctagccggag ccaggctcga ccccgagtcc tcggacgacg tttccacaat aagggcttac
     2821 gagacgttca atcagatggt ggacaggatg aagaagaggg aaagcatcga ggaagacctg
     2881 ccgccgttcc agccgacttt aatttcagcg gacgacgaag acgaggaaga ggaaaccatt
     2941 tcgaggccca gaaacgtgac cgtcgtggtc gcgaagatcg ccgacttcca ggagctctcg
     3001 aacactctca agccggccga gttcaacgcg ttcctggccg attacaggaa ggccgcgtct
     3061 gccatcatct ccaattacgg aggcgtgatc gaggcgcttt taaaggacga gatagtggct
     3121 ttcttcaacg caccagaaga gcaggtaaac ccggagctca aggccgtctg ctcggcagtc
     3181 gaggtgcttc agctcctggc cagcatcacc agggagcgca agatagaggg caaggcggta
     3241 ataagcggca agatcggcat agggatgaag tccgtccccg tttacgacga ctcgagcatg
     3301 ccccacggcg taaagcaggt gaccgacatg gcgcgcagga tctgcaccgt cgcacccgta
     3361 tggagggtga tagtctccgc cgacgtttac cagatcgtga aggacttcgt cgaagcccgc
     3421 gagctcaagc tcgggaacga accctactac tccatagtgg gcgtcgaaga gggtgtggtc
     3481 tgaaaactgg ccgttttacc cgctctcgtc tcttcctttc cccaacatta agaaccaact
     3541 tgactccgac aatttcgtat gtctgcggcg acacgccgcc cccatttgtc tcattttcac
     3601 gattcatgta atctttcttg accgttatgg acaaaaagcg catagtcgtc gccatgagcg
     3661 gcggcgtgga cagcacgaca gtagcg
//
LOCUS       DASSJH010000174         6508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_365779, whole genome shotgun sequence.
ACCESSION   DASSJH010000174 DASSJH010000000
VERSION     DASSJH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365779"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..742
                     /locus_tag="VFJ67_03725"
     CDS             227..742
                     /locus_tag="VFJ67_03725"
                     /inference="COORDINATES: protein motif:HMM:NF037752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7289524.1"
                     /translation="MIKKKFIKEELQLIKLIFPILLTVLFAAHHAFAQIPSCPCDTLE
                     FPDGNTGDSIVEILCPGGNLASGNDSIVTQDEVLIATPEGETPLLGYHVGIAKGDFKF
                     CQVSDDGISNVPIELSDQEFENCKDSLVERCSLGHNPIPTLSEWGMIAMAGVLGMIGL
                     YIAARRRKAAA"
     gene            1269..2180
                     /locus_tag="VFJ67_03730"
     CDS             1269..2180
                     /locus_tag="VFJ67_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET7289525.1"
                     /translation="MGLNRSFIGKEYEPSVHVVSTEDVIDYAWAIGARNLSYFNYNGA
                     FNFSHESPVGMAPPSYAVTYELPMLEQVWSDPELHGGVEEAKKNVLMLVHGDQSMKFY
                     KPIRPGDKLTYRTKIVDIQDKGSGELLKINVLTTNGKGEKVVESDWGLFIRGIGSGEK
                     PKTAASNGQPKEAPAEPPLAFRDIIRVPDDITYRYSKASNDMNPIHIDEKVAKEAGLS
                     GIIVHGLCTMSMTMRAIIESYLDSDPSKLKSLGVRFTAPVYPGDTIIVDGWEKGRENG
                     HVVIGFDVTRKSDNVKVIKGGTAEVEI"
     gene            complement(2249..3934)
                     /gene="hflX"
                     /locus_tag="VFJ67_03735"
     CDS             complement(2249..3934)
                     /gene="hflX"
                     /locus_tag="VFJ67_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET7289526.1"
                     /translation="MHISKIYGKTSGLTRSETGGLERLYRRKIPPKEIITLELARTLT
                     RLTHELRRGISVMIDRKGKVSAIFLGDTHEIPFDEILGRSREAKFRLRGHRLVHTHLK
                     NSGLSEHDLATLVNERLDLIGLLEVRPDGDPGKFYLAHVLPANHDGSKWRIEEYRDLG
                     QLDTACDELIRDVEQSLEKSYYEQGGAKGREGVLLVGFSTKYRSEAEESLEELKSLAK
                     SANKAVLGTVIQIRKKMDPRYLIGKGKLNEAILTAKQVGAEKIIFDVELTPAQVKSIT
                     DETNLVVMDRTQLILEIFARHATTSEGKLQVKLAQLKYNLPRLVGRGIELSQLGGGIG
                     TRGPGEKKLEEERRRIRKMIENLEKEITQLSKRRERTREKRKETGIPTVTFIGYTNVG
                     KSTLFNALTNSGVVVENKLFSTLVPTTRKIMLPRGREILITDTVGFISDLPKELVNAF
                     RATLEELGGSSLLLHVADASDPMVDERIESVEKILAQTGYESIPQMIVLNKADKTPPE
                     VARRLGRAYGAPLISAREKNAIPEMLGLIEKRIFGETPEDEAEGRMALSLHTA"
     gene            complement(4361..5197)
                     /locus_tag="VFJ67_03740"
     CDS             complement(4361..5197)
                     /locus_tag="VFJ67_03740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7289527.1"
                     /translation="MNKHENAIQSHYGQEGLSDRILSALASAGKEIRTPADTSTFDEF
                     HIRGHEATLEIGLLAELGRGMKVLDMGSGIGGPARTIAAEFGCRVTGIDLVDEYIRTA
                     EMLTGMVGLGDKVTFRKGDMTDLPFDAGSFDRALTIHTQMNVRDKAKLFGEVSRVLSH
                     GGRFALYEICAGPNSPARFPVPWADDPSISFLITPVELREMLGREGFKELEWRDVSAK
                     SLEWFRSEMEARASAPEDAPPPLGLNLIMGPSFAEKLENVFKNLKEDRIRVVMGVFRK
                     GE"
     gene            complement(5329..>6508)
                     /locus_tag="VFJ67_03745"
     CDS             complement(5329..>6508)
                     /locus_tag="VFJ67_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289528.1"
                     /translation="AWTIALKFTGGGLDAPGAVSIDEDGNVWATNDLLYGPQPELSAC
                     GDGGSGVTKLTPDGYPLSSALGFKGGGLDGAAYGLAVDQTGNVWIGNYHGGGVSLLTP
                     DGSPLSPDVMAYRAGGNAGRVQGTIVDYEGNVWFVNSGPSSACQACGNTLTLFPGGDP
                     DNAVTFAYPGTDTLNEPYGVALDLDGEIWITNAGGNTVTRIDSLGNPDFQSEAECCGI
                     SGPRGIAIDSIGNVWIANLTDPGGSEGGSVTLLDPFGSNAPGSPFRDGGIRGAWGIAV
                     DGADNVWVANFIGGTITNLCGVTSESCPQGYNTGDPISPSSGYDGGGALQHPTAVAID
                     QAGNVWAANNVNDLEACLRMSESGCPQASNECEGDGLVVFYGLATPVDAPLIGPPRQP
                     "
BASE COUNT         1355 a         1844 c         1729 g         1580 t
ORIGIN      
        1 atgatcggat agtacgtggc ttcgcgccgc aggaaggcaa ctgcgtaaac taggcttata
       61 ataaagcaaa caaaagggag ctccggctcc cttttgtttg cttctggcag ccggaacaac
      121 gtccggtcct agcacgtaat agaaccttcc caacctcccg gtaattattc taaaatcgta
      181 ccttgtagtg caaatccggg agtgatgtaa tcatcccgat ttatatgtga tcaaaaagaa
      241 atttataaag gaggaattac aattgataaa gctgattttc ccgattttat taactgttct
      301 gtttgccgcg catcacgcat tcgcgcagat tccttcttgc ccgtgcgata cccttgaatt
      361 cccggatggt aacactggcg actcaatcgt cgagatcttg tgccccggcg gtaatctggc
      421 ttcaggtaac gattcaatcg taactcagga tgaagttctg atcgcaactc ctgaaggtga
      481 aactccgctg cttggctatc atgtaggtat tgcaaaaggc gacttcaagt tttgtcaggt
      541 ttccgacgat ggcataagta atgtaccgat tgagctttcc gaccaggaat tcgaaaattg
      601 taaggacagc ctggtcgaac gatgcagcct gggccataat cccatcccta cgctctccga
      661 atggggcatg atcgcgatgg ccggcgtcct tggcatgata gggttatata tagctgctcg
      721 cagaaggaaa gcggctgctt aaacacggac ataacgcatt aagatagagt aacaaaaagg
      781 gagcttcggc tccctttttt gttcctacat ctgtttctgt ttctctgcac atctgcgaca
      841 ttttaagtcg atggcgctca atcatttcat aacacgccct gtttattcag tctttaccaa
      901 ttcccttcgc cgctgaccgc gttataagga caaatccggc tcaagattcg aattagcgtc
      961 caaaaaaacg gcgaaaagtt tttgcaaatg cgaatagccc ccttgacaaa accacccttt
     1021 cctgattatt ttatatatca ggaatgaacg tgcctcaatg tggaacttat cggagatttg
     1081 tggataagac aatgattgca ggctgtttaa ctgtactccg cgacatatat cttttttctg
     1141 tttcctgttt ttccgggttc ccggggaatt cgcaattctg taagctaacg cagtatatag
     1201 agttataaaa gagctaatta tcatacgagg ccggcggcgg attacacacg taggcaggga
     1261 gattcaagat gggactcaac agaagcttca taggaaagga atacgagcct tccgtgcacg
     1321 ttgtcagcac ggaggatgta atagattacg catgggcgat aggggccagg aacctcagct
     1381 atttcaatta taacggggca tttaatttca gccacgagag cccggtcggg atggccccgc
     1441 cgagctacgc ggtaacctac gagctcccga tgctggagca ggtttggagc gacccggagc
     1501 tccacggagg cgtcgaggag gccaagaaga acgtcctcat gctcgtccac ggcgaccagt
     1561 cgatgaaatt ctacaagccc atcaggcccg gagacaagct cacctacagg accaagatcg
     1621 tggacattca ggacaagggc tcgggcgagc tgctcaagat aaacgtgctc actacgaacg
     1681 gcaagggcga gaaggtagtc gagtcggact ggggcctttt cataaggggc atcgggagcg
     1741 gtgagaagcc caagaccgcg gcgtccaacg gccagccgaa ggaagccccg gccgagccgc
     1801 ctctcgcgtt cagggacata atccgcgtac ccgatgacat cacgtacagg tattcaaaag
     1861 cgtctaacga catgaacccc atacacatag acgaaaaggt agccaaagaa gccggcctga
     1921 gcgggataat agtccacggc ctctgcacca tgtccatgac tatgagggcg ataatcgaga
     1981 gttatctgga cagcgacccg tcgaagctca aatcgctcgg cgtcaggttc acggccccgg
     2041 tttaccccgg cgacacgata atcgtcgacg gatgggagaa agggagagag aacggacacg
     2101 tagtgatagg gttcgacgta acgagaaaga gcgataacgt taaagtaatc aaaggcggta
     2161 ctgcggaagt agagatatag tactaccttg acttgctttg atgattgatg agtgaacgag
     2221 cggcggcacc aggaaccgcc gctttttttt acgctgtatg caggctgagc gccatccggc
     2281 cctcggcttc gtcttcaggg gtttccccga atatcctttt ctctatgagg ccgagcattt
     2341 cgggtattgc gttcttctcc cgagccgata tgagcggcgc gccgtaagcc ctgccgagcc
     2401 tccgggcgac ttcgggcggg gtcttgtccg ccttgttgag cactatcatc tgcggtatgg
     2461 attcgtagcc cgtctgcgcg agtatcttct ctacggactc tatcctctcg tccaccatcg
     2521 ggtcgctcgc gtcggccacg tgcagcagca gcgacgagcc tccgagctct tcgagagtcg
     2581 cccggaacgc gtttacgagc tccttcggca ggtcgcttat gaaacccaca gtgtccgtta
     2641 tcagtatttc tctccctctg gggagcatta tctttctcgt ggtagggacg agcgtggaaa
     2701 agagcttgtt ctccaccacg acgccgctgt tcgtgagcgc gttgaagagg gtcgatttgc
     2761 cgacgttcgt atagcctatg aacgtgacgg tcggtatgcc ggtttccttc ctcttctccc
     2821 tggttctctc tctcctcttg cttaactgcg tgatctcctt ctcgaggttc tcgatcatct
     2881 tcctgatcct tcgcctctcc tcttcgagct tcttttcacc ggggcccctc gttccgatac
     2941 cgcccccgag ctgcgatagc tcgatcccgc gcccgacgag gcgcggcagg ttgtacttga
     3001 gctgggcgag tttaacttgc agcttccctt ccgaggttgt cgcatgtctt gcgaatattt
     3061 ccaatatgag ctgggtcctg tccatgacga cgaggttagt ctcgtccgtg atagacttca
     3121 cctgcgcggg cgtgagctct acgtcgaata ttatcttctc cgcgcctacc tgctttgccg
     3181 tgagtattgc ttcgttcagc tttcctttcc cgatcaggta cctcgggtcc attttctttc
     3241 ttatctgaat cacagtgccg agaacggctt tgttggctga cttcgcgagc gatttaagct
     3301 cttccagtga ctcttccgcc tcggagcggt atttcgtcga gaagcctacg agaagcactc
     3361 cttcccggcc tttcgctccc ccctgctcgt aataggattt ttcgaggctc tgctcgacgt
     3421 ccctgattag ctcgtcgcag gcggtatcca actggccgag gtccctgtac tcctcgatgc
     3481 gccacttgct gccgtcgtgg ttggcgggca ggacgtgcgc gagatagaac ttgccggggt
     3541 cgccgtcggg tctcacttcg aggagtccga tcaagtcgag acgctcgtta acgagggtcg
     3601 cgaggtcgtg ctcgctcaac cccgagttct tgagatgcgt atggacgagc ctgtggcccc
     3661 tcagcctgaa tttggcctcc ctcgacctgc cgagtatctc gtcgaacggg atctcgtgcg
     3721 tgtcgcccag gaatatggcc gataccttcc ccttcctgtc tatcatgacg gagatgcccc
     3781 ttctaagctc gtgcgtgagc ctcgtgaggg ttcttgccag ctccagcgtt attatttctt
     3841 tcggggggat tttccttctg taaagtcttt caaggccgcc tgtttcactt ctcgtgagtc
     3901 ctgatgtctt tccgtatatt ttacttatat gcactcctcc tgagcgtatt taccactact
     3961 cttatgataa acattcgccc gaatcagtca aggagattaa tattccctta atcaataatt
     4021 ttcgagtcat tccggcaaat tcaggaaaag gcaaaatcgc cgcgtccatg atcctgaggc
     4081 aagttcagga caggccgcct cgagatgacg gaataagagc tgtcattgag agcaaagcga
     4141 agcaatctca tcctttgcat ttaattccct ctcctggagg ggggaagggt cgtgggcggg
     4201 gacgtaacct tctaatttac gattttagag atcccgttcg agccgaaccc gtcgaaatat
     4261 atcatataaa tgtaagcaag agtggtggat aaaactgata cttaaaccat ctcctccggc
     4321 cgtcccggtg gggcgtcagt tcataggcag tcccgatatg tcattccccc ttcctgaata
     4381 cgcccatcac aacccgtatc ctgtcttcct tcaggttttt gaatacattt tcgagcttct
     4441 cagcgaagga cggtcccata atcaggttga ggccgagagg aggcggggcg tcttcgggcg
     4501 cggaggcacg cgcttccatc tcggaccgga accattcgag agacttcgcg ctcacgtccc
     4561 tccattcgag ctctttgaac ccttcccgtc cgagcatttc cctcagctcc acgggcgtga
     4621 ttaaaaaact tatcgacgga tcgtcggccc acggtacggg gaagcgcgct ggagagttag
     4681 gtcccgcgca tatttcgtat agcgcgaacc ggccaccgtg ggagaggacc cttgagacct
     4741 cgccgaacag tttagccttg tcccgcacgt tcatctgagt gtgtatcgtt aacgccctgt
     4801 cgaaagagcc ggcatcgaaa ggcaggtccg tcatgtcgcc ctttctgaac gtcactttgt
     4861 ctcccaggcc gaccatcccc gtcagcatct ccgccgtccg gatgtactcg tcgacgagat
     4921 ctattcccgt gacgcggcag ccgaattcag ccgcgatcgt gcgcgccggc cctccgatac
     4981 ccgagcccat gtcgagcact ttcataccgc gtccgagctc cgcgagcagt ccgatctcga
     5041 gggtcgcttc atgccccctg atatgaaatt cgtcgaatgt ggacgtatcg gcgggggttc
     5101 taatctcttt tcctgcgctt gctagggccg aaagtattct gtcactgagg ccttcctgtc
     5161 cgtaatggct ttgtatcgca ttttcgtgtt tattcatagc tcgtaccttt ggttttggtc
     5221 tgtgcggcgg gatttatttt aagtctattg gatagggaag ggtgtatcaa ctagtttttg
     5281 ttccgaatgg gatttagaga tgcttatata gaagactttc gacgttccct acggctgtct
     5341 cggagggcct atcaggggcg cgtcaacggg agtcgcgagg ccgtagaaca cgacgagccc
     5401 gtcgccctcg cattcgttag aagcctgcgg gcatccgctc tcggacatcc tgaggcacgc
     5461 ctctaagtcg ttcacgttat tggcggccca cacgttcccc gcctggtcta tcgccacagc
     5521 cgtcgggtgc tgaagcgccc ccccgccgtc atatcctgac gagggcgata tgggatcccc
     5581 cgtgttatac ccctgggggc agctctcgct cgtaactccg cataagttcg tgatcgtgcc
     5641 gcctatgaaa ttggcgaccc atacgttatc cgcgccgtcg acggcgatac cccacgcgcc
     5701 ccgtattccc ccgtccctga agggcgagcc gggcgcgttc gagccgaagg ggtcgagaag
     5761 cgtcacgctc ccgccctcgg agccgcccgg gtcagtcagg ttcgctatcc agacgttccc
     5821 gatgctgtct atcgcaatac cccttggccc gcttatgccg cagcactccg cttccgactg
     5881 aaagtcgggg ttcccgagcg agtctatgcg cgtaaccgtg ttcccgccgg cgttcgttat
     5941 ccagatctct ccatcgaggt cgagagcgac gccgtagggc tcgttaagcg tatccgtccc
     6001 cgggtatgcg aacgttacgg cgttatccgg gtcgccgccc gggaaaagcg taagcgtatt
     6061 gccgcaggcc tggcacgcgc tcgaaggccc gctgtttacg aaccagacgt tgccttcgta
     6121 atcgactatc gtgccctgca ccctgccagc gttgcctccg gccctgtatg ccataacatc
     6181 gggcgatagc ggggagccgt cgggagtgag cagcgacacg ccacccccgt gatagttccc
     6241 gatccacacg ttccccgtct gatctacggc gagtccgtaa gcggctccgt ctaaacctcc
     6301 tcccttgaat ccgagcgcgg aggataacgg atagccgtcg ggagtgagct tcgtgacgcc
     6361 cgagcctccg tcgccgcagg cggagagctc cggctgcggg ccgtaaagaa ggtcgttggt
     6421 cgcccaaacg ttcccgtcct cgtctatcga tacggcgccg ggagcgtcga gaccgccgcc
     6481 cgtgaatttg agcgcgatcg tccatgcc
//
LOCUS       DASSJH010000175         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1339048, whole genome shotgun sequence.
ACCESSION   DASSJH010000175 DASSJH010000000
VERSION     DASSJH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339048"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFJ67_03750"
     CDS             complement(<1..412)
                     /locus_tag="VFJ67_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7289529.1"
                     /translation="MREPESKMHERERKLFKEAVKIAGKAGKMLLGSFRKERPVERGT
                     VKEVKLVYDLVADSIIRKEIESRFPGHSYVTEETGFVDKESDYLWIIDPLDGTSNFAD
                     QNPMFAVSISLWHGGEPLLGVIEAPMMMERFAAVK"
     gene            489..767
                     /locus_tag="VFJ67_03755"
     CDS             489..767
                     /locus_tag="VFJ67_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289530.1"
                     /translation="MGGTYLEGEKALERLGDLAELICRIAEGSGLENEDEELLEGLLL
                     EMGAALRERDLPGAKKIVARMLSEVLGYDEVRAGFVMRNHLNKNAGWG"
     gene            complement(890..1105)
                     /locus_tag="VFJ67_03760"
     CDS             complement(890..1105)
                     /locus_tag="VFJ67_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289531.1"
                     /translation="MVTRKKEKTKKEPLEKAIYVKASQEFVDMLNESQWHLQMRPAQI
                     VREAVTEYLKKHLPKEARDKYFKTTAK"
     gene            1475..2563
                     /locus_tag="VFJ67_03765"
     CDS             1475..2563
                     /locus_tag="VFJ67_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7289532.1"
                     /translation="MKWSWKIGEFAGIGVYMHATFVLIIAWVILIHWSAGHDLATIVS
                     GVVFVLALFLCVVLHEFGHALTARRFGIRTRDITLLPIGGVARLERMPDDPMQEFWVA
                     LAGPAVTVAIALLLFFWLRITGTFVPVDKITMTQGSFVERLMLVNVFLFVFNMIPAFP
                     MDGGRVLRALLALRTDYTSATQTAATIGQAIALIFGFVGLFTNPFLIFIALFVWIGAA
                     QEASMVKIKSALGGIPVYKAMLTDYKTVTSGDSLKDIVDLTMSGSQQDFPVVDNGRLV
                     GIVPHADFIKGLAEKGDTVPVGVIMRREFETADPSEMLEYAFARLQTGDFYTMPVVKN
                     GELVGLLTMDNVGEFMRIQSAMSGPRGR"
     gene            2755..>3442
                     /locus_tag="VFJ67_03770"
     CDS             2755..>3442
                     /locus_tag="VFJ67_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET7289533.1"
                     /translation="MSCAGCVETVERTLRSLPGVSDAQVNFAERTATVTGDVSPDALV
                     SAVGRAGYTASLLEDEDAESEKENAELKHYRDLLRRTVVSGVISVIIFAVSMFGLLPS
                     VDSPSGRISWGVASLLTLFVLAYGGGRFFTGALKSFKNHNANMDTLIAVGTGVAWVYS
                     TFVVLFPGSLSEIARHLYFETAALIIAFINLGSALEMRARGKTSQAIKRLIGLQPRTA
                     RVLRDGQEVDI"
BASE COUNT          732 a          894 c          969 g          847 t
ORIGIN      
        1 cctttaccgc ggcgaacctc tccatcatca tcggcgcttc tataaccccc agaagaggct
       61 ctcctccgtg ccagagggat attgagactg cgaacatggg gttctggtcg gcgaagttgc
      121 tcgttccgtc gagcgggtct attatccata ggtaatcgga ttctttatcg acgaaccccg
      181 tttcctccgt tacgtacgag tggcccggga aacggctttc aatctctttc ctgataatac
      241 tgtcggcgac caaatcgtat acgagcttta cctctttgac cgttccccgt tcgaccggcc
      301 gctctttcct gaagctcccg agcagcatct tccccgcttt cccggctatc ttaacggctt
      361 ccttgaataa ttttctttcc cgctcgtgca ttttgctttc tggctctctc atgggttcgc
      421 tccccggtca acgttcttac atttgccgga ataatgtaga attaataata tctgattcag
      481 ggggagtaat gggcggcacg tacctcgaag gggaaaaggc gcttgagcgg ctaggagacc
      541 tggcggagct catctgcagg atcgcggagg ggagcggctt ggagaacgag gacgaagagc
      601 tgctggaagg actccttctg gagatgggcg cggctctcag ggagagagac ctccccgggg
      661 cgaaaaaaat tgtggcaagg atgctctccg aagtgctcgg ttacgatgag gtccgcgccg
      721 ggttcgtcat gcggaaccat ctcaacaaga acgccgggtg gggatgattt aatagtctgg
      781 cttgcccctg ttcgcgtagt gaggcgtaaa agccctgtta cagcatgtta atggacggta
      841 tgtcagatat atttacagtg gttgatccgc cggtatacga gcggacatgt tatttcgcgg
      901 tggtcttgaa atacttgtcc cttgcttcct tggggaggtg ttttttcaga tattccgtta
      961 ccgcttccct cacgatctgg gcaggtctca tctggaggtg ccactgtgac tcgttgagca
     1021 tatccacgaa ttcctgggac gccttgacgt atattgcttt ctcgagcggc tctttcttgg
     1081 ttttctcttt ctttcttgta accatatttt aaatatataa catagtcgag atataaaatg
     1141 aatagacgat tttccgctcc ggcagaaatg tccgcggtga attaaagcgg cgataaatgc
     1201 gtcccggaaa attataatgt tatatgtata ttatcgtggc gcggggcggt gtcatgagct
     1261 catggcgtat ctgcgtccgg gagggcgctc gccgtgactt ccgtattgac atcgcgaaag
     1321 cagcgaataa attttaattg atgtataaag acgacgactc cttatacggt acaatttacg
     1381 tggtgagcat cagacggcat gggcacatat cgaagaatct aacaggtctt tacggtgtca
     1441 taatgaaaag ggtttcatac ggaggggacg taccatgaaa tggtcctgga agataggcga
     1501 gttcgcgggc ataggggttt atatgcacgc gactttcgtg ctcataatcg cctgggtgat
     1561 actcatacac tggagcgcgg ggcacgacct cgcgacgata gtctccggtg tcgtttttgt
     1621 cctggcgtta ttcctatgtg tcgtgctcca cgagttcggt cacgcgctca ccgcccggag
     1681 gttcggcata aggacgcgcg acatcacgct cctgccgatc ggcggagtag ccaggctaga
     1741 gcgtatgcct gatgacccga tgcaggagtt ctgggtggct ctcgccggcc ccgccgtgac
     1801 cgtggcgatc gcgcttctgc tctttttctg gctccggatt accggcacct tcgttcctgt
     1861 cgataaaatc accatgaccc agggttcttt cgtcgagcgt ctcatgctcg tgaacgtatt
     1921 cctctttgta ttcaatatga tccccgcgtt cccgatggac ggaggacgtg tgctgagggc
     1981 gctgctcgcg ctaaggaccg attatacgag cgcgacccag acggcggcca cgataggaca
     2041 ggcgattgcc cttatcttcg gcttcgtcgg tctctttacg aacccattcc tgatcttcat
     2101 agcgcttttc gtatggatag gggccgccca ggaggcgagc atggtaaaga taaaatcggc
     2161 gctcggcggt atcccggtgt ataaggcaat gctcacggat tataaaaccg ttacttccgg
     2221 cgacagtctc aaagacattg tcgatctcac gatgtcgggt tcacagcagg actttcccgt
     2281 ggttgataac ggcaggctcg tcggaattgt tcctcacgcc gactttataa aaggtctcgc
     2341 cgagaaaggc gacactgtcc cggtgggggt tatcatgagg cgcgaattcg agaccgctga
     2401 cccatccgag atgctcgagt atgcgtttgc ccgccttcag acgggcgatt tctatacgat
     2461 gcccgtcgta aagaacgggg aactggtcgg actgcttacg atggacaacg tgggggagtt
     2521 catgaggatt cagtccgcca tgtcgggccc gcgcgggcgc tgaaacggac aaatccgggt
     2581 cattataatg cgggcaatgt taagccccgc cccgaaaatc acccggcaca agtggtatca
     2641 tattcacgtc tgattgattc ggtatcgatc cgcgttacag gtgaaaatat ggaagaaaaa
     2701 acggaaattc ggaaaggcga tcggaatacg acccgccgtc tccacgtgac ggatatgagc
     2761 tgcgccggat gcgtcgaaac ggtagagagg acgctcaggt ccctaccggg ggtatcggac
     2821 gcgcaggtta acttcgccga gaggacggcg acagtcacgg gcgacgtctc cccggacgcg
     2881 cttgtcagcg ccgtcggaag ggctggttac acggcctcac ttctcgaaga cgaagacgcc
     2941 gaatccgaga aggagaatgc ggagttaaaa cattatagag acctcctccg ccggacggtc
     3001 gtctccggtg taataagcgt aataatattc gctgtaagca tgttcggtct cctgccttct
     3061 gttgattcgc cttccgggcg gatttcgtgg ggcgtggcat cgctgctcac gctgttcgtc
     3121 ctcgcgtacg ggggaggccg ttttttcact ggagctctga agtccttcaa aaaccataac
     3181 gctaacatgg atacgctgat cgccgtcggc acgggagtcg catgggtcta ttcgaccttc
     3241 gtcgtactct ttcccggctc tctctcggag atagcgagac acctctactt cgagaccgct
     3301 gctctgataa tcgcgttcat aaatctcggc tctgcgctcg agatgagggc gcgcggcaag
     3361 acctctcagg cgataaaacg cctcataggt ctccagccaa ggactgcccg ggtactgaga
     3421 gacggtcagg aggtcgatat cc
//
LOCUS       DASSJH010000176         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1553787, whole genome shotgun sequence.
ACCESSION   DASSJH010000176 DASSJH010000000
VERSION     DASSJH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553787"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..82
                     /locus_tag="VFJ67_03775"
     CDS             <1..82
                     /locus_tag="VFJ67_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289534.1"
                     /translation="IINEVRGVNRVVYDVSTKPPSTIEWE"
     gene            complement(112..321)
                     /locus_tag="VFJ67_03780"
     CDS             complement(112..321)
                     /locus_tag="VFJ67_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289535.1"
                     /translation="MKWDYRVISVDKLFQGSEDHDIAVSKEAATARRNKVGQGMESTL
                     NELGGDSWELVTIAGDFGIFKKPRS"
     gene            complement(327..518)
                     /locus_tag="VFJ67_03785"
     CDS             complement(327..518)
                     /locus_tag="VFJ67_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289536.1"
                     /translation="MKKKEEFIYDVRVTSRHIREGVITKKDYDKHLSELPNVEEKSEP
                     LIIEDEDGKAGGEPESGEE"
     gene            complement(638..1576)
                     /locus_tag="VFJ67_03790"
     CDS             complement(638..1576)
                     /locus_tag="VFJ67_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7289537.1"
                     /translation="MKILLTGGAGFIGSWVAERYIKEGHDVLILDDLSTGRMENIPEN
                     AAFIKCDIRDDDAVRRAFAEFRPDVVNHHAAQIDVRKSVEDPAYDASVNIIGTLRLLE
                     SSVRNGVKRFIFASTGGAIYGEPANIPADEKTPPMPISPYGTSKYAVEKYLEYYRHIY
                     SLDSVALRYANVYGPRQNPHGEAGVIAIFCSRILSGQPCMVFGDGEQTRDYVYAGDVA
                     RANLCALSSPSGSYNIGTQIETSVNGLITELEAASGIKFTVQYAEARAGEVGRISLDV
                     RLAGKVLDWAPSVFLAKGIKETWEWFKAGYAGADKS"
     gene            2038..>2474
                     /locus_tag="VFJ67_03795"
     CDS             2038..>2474
                     /locus_tag="VFJ67_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HET7289538.1"
                     /translation="MGRNNLKESSHALTTGVLGSSHDFGRHRSMPGDFAYEQNIMSVA
                     ATGKSNGKQRVIPDEDYKLLQSYFREVGTESLLTATDEIRIATKIKKYNAMARRLEAM
                     IGRLTEGSRGVKAQKVCECRRTGKKSAAGGKAGCAPRPSLKSTL"
BASE COUNT          577 a          683 c          619 g          595 t
ORIGIN      
        1 catcatcaac gaggtcaggg gcgtaaaccg tgtggtatac gacgtatcga ccaaaccccc
       61 cagcacgatc gagtgggaat gaaacggtga gaggccgtac cgccttaaat ctcagcttct
      121 gggtttttta aatattccga agtccccggc gatcgtgacc agctcccacg aatctccgcc
      181 gagttcgtta agtgtgcttt ccatgccctg cccgacctta ttccttctgg cagtcgcggc
      241 ttctttcgat accgctatat cgtggtcctc gctgccctga aacagcttgt caactgaaat
      301 aaccctatag tcccatttca tcaagttcat tcctctccgc tctcgggctc tccacctgct
      361 ttcccgtctt cgtcttcgat aataagaggc tcgctcttct cctccacgtt gggaagctcg
      421 ctcaaatgtt tgtcgtaatc cttcttcgtt atcacaccct ctcttatgtg cctgctcgtg
      481 acccttacgt catatataaa ttcttctttc ttcttcatat aattgaacct ccgaggtgat
      541 taaggatacc ttaagagccc ggatacgcaa tcgaacgcgg ctgccgcctg aatccagctc
      601 cgtaccaggc cggccagggc gaagggaggg cccgctctca ggatttatcg gctcccgcgt
      661 aaccggcttt gaaccattcc caggtctctt ttatgccctt ggcgagaaag acactcggcg
      721 cccagtcgag gaccttgccg gcgagtctca cgtcgaggct tatcctgccg acctccccgg
      781 cccgcgcctc cgcgtactgt accgtgaatt tgatcccgga ggccgcttcg agctcggtta
      841 tgagtccgtt caccgaagtc tcgatctgtg tccctatgtt gtagctgccg gagggggacg
      901 agagggcgca caggttcgcc cgcgcgacat cgcccgcata aacgtagtct ctcgtctgtt
      961 caccgtcacc gaataccatg cagggttgtc ccgaaaggat gcggctgcag aatatcgcga
     1021 ttaccccggc ttccccgtga gggttctgtc tcgggccgta cacgttcgcg tatctgagag
     1081 cgaccgagtc gagggaataa atgtgcctgt aatactcgag gtatttctcg acggcatact
     1141 tggatgtgcc gtaaggcgat atgggcatgg gcggggtttt ttcatcggcg ggtatatttg
     1201 cgggctcgcc gtatatagca ccgccggtcg atgcaaatat gaaccgtttt acgccgttcc
     1261 tgaccgagga ctcgagcagc cttagagtgc cgatgatatt gactgacgcg tcgtaggcgg
     1321 ggtcttcgac ggatttcctc acgtcgatct gggccgcgtg gtggtttacc acgtccggac
     1381 ggaattcggc gaatgcccgg cggactgcgt catcgtcacg tatatcgcat ttgatgaacg
     1441 ccgcgttctc gggaatattc tccattctcc cggtcgaaag gtcatcgagt atcagtacat
     1501 cgtggccctc ctttatatat ctctccgcaa cccaggatcc gataaatccg gcgccgcccg
     1561 taagaagaat cttcatttcg acaatccccc gctaaaaaat aattttggta gagaatctac
     1621 ccgggtacgt tgtaccgacc ccgcttatat ttactttatt cgaagtggtt gtcaacaaaa
     1681 cgtaagcggc agttccgcaa accgcgataa caatcaatta tcatttcccc gttgtgtaaa
     1741 tacaattatt taaacagcaa tgcccggatc ttataatatc acccagaaag ccgaatcggg
     1801 gcacgtgccg tgatattttt ggtaaccgga gccaacacat tttgttttca atttccggcc
     1861 aattattcgg acaatgacat tttttgtcaa taaacccctc cggggcagag gaaacgaagc
     1921 gactgtcgga attccaatta atgaatgata acagataatt ggagtaaata tttgttttat
     1981 ggcatgaaca ttgcatccga atctatccga ggattaaaaa atcggaggat caatcaaatg
     2041 ggtcggaaca acttaaaaga atcgtcacac gcgttaacta caggcgttct cgggagcagc
     2101 catgatttcg gcaggcacag gtcgatgccg ggtgattttg cctacgagca aaacatcatg
     2161 agcgtcgccg ctaccggaaa gagcaacggc aaacaaaggg tgatccccga tgaggactac
     2221 aagctccttc agtcgtattt cagggaagtg ggaacggaat cacttctcac ggcgaccgac
     2281 gagatccgaa tcgcaacgaa gatcaagaaa tataacgcga tggcccggag actcgaggcc
     2341 atgatcggca gacttaccga agggagccgc ggcgtgaagg cccagaaggt atgcgaatgc
     2401 aggcggaccg ggaaaaaatc cgcggccggc ggcaaagccg ggtgcgcacc ccgcccctcc
     2461 ttaaaatcta cact
//
LOCUS       DASSJH010000177         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_474377, whole genome shotgun sequence.
ACCESSION   DASSJH010000177 DASSJH010000000
VERSION     DASSJH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474377"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1077
                     /gene="recO"
                     /locus_tag="VFJ67_03800"
     CDS             370..1077
                     /gene="recO"
                     /locus_tag="VFJ67_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET7289539.1"
                     /translation="MKSSEAFLLRKRPYGDADYVLTLFTREYGKISAFAKNAKTSRKR
                     FGGRLEPFVLFRVRFRERSGDMKFVEDVETIEVFRRLMEDIELFMWGSFLSESVEILL
                     PKESPNEDLFSLFTDTLRQIDSGKSAMPLVLGFQLGALSLSGYGPSLDACAECGGETG
                     KGARFSIRKGGALCAGCAEGLQNGTPYSGDFLKDRESMEIQLGRVLRYIKLFSKFTEY
                     HTEKKFNSSKFIEELKI"
     gene            1074..2252
                     /gene="argJ"
                     /locus_tag="VFJ67_03805"
     CDS             1074..2252
                     /gene="argJ"
                     /locus_tag="VFJ67_03805"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET7289540.1"
                     /translation="MIRVPGFAGSGISCGIKKRGKKDLALLFSEKPARAAGVFTTNIV
                     KAPPVLVGMERIKSGLCQAVLVNSGVANAFTGKAGMKTALLTTECAADELGIDKSLVI
                     PSSTGVIGGQLPVDKINKAMPHLVSELREDGLEDAASAIMTTDAFPKLAITKVGIGGR
                     TGTICVIGKGAGMIMPNMATMLAYILTDINVGRRTLTKALGNVVNNSFNKIIVDGDTS
                     TNDTVLILANGKLGNTALGLTGTLYRKFEQAVTEAASEVAEMIVKDGEGATKTVKIIV
                     IGAKSVSDAESVARTIGTSLLVKSALYGEDPNWGRIVAAAGRAGVDFDPDRLDLYIGD
                     HKVLRNSKEVMDEARALEIMKTPKFSITLDLKTGKRASFVITSDITYDYLKINAHYRT
                     "
     gene            complement(2321..3424)
                     /locus_tag="VFJ67_03810"
     CDS             complement(2321..3424)
                     /locus_tag="VFJ67_03810"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET7289541.1"
                     /translation="MYKEIIRPILDRLDSETFHNIARESLHLAELTPLTLGLVELFAD
                     GHKRFTDPRLRTTLGGIEFENPLIVGAGWDKSGRALKGLWRLGFAGVEVGSVLEYRQP
                     GNPKPRQFMLSPGVALNWLGFNSPGMDVVAKNLSRYEGAPVRIGISIGMNRDVPHDDS
                     PRAHAVVAERLYGYADYFAINVSSPNTPGLRMLHEKGRKRDIIQAVNDAMESRGGRKP
                     LFVKIAPELGFKEVDDILEVAVEHSITGIIAANTADVPEMKAKYGERWKSQAGGLSGD
                     DEDYRRMTTEKVAHVYKQAGDKLEIIGVGGIKDAETALEKIRAGARALQIVTAIRGEG
                     TAVSGKINRGIVEYMEREGVKSLDELIGVDEGS"
     gene            complement(3417..4517)
                     /gene="ychF"
                     /locus_tag="VFJ67_03815"
     CDS             complement(3417..4517)
                     /gene="ychF"
                     /locus_tag="VFJ67_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET7289542.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNALTNAGAEMGNFPFTTIEDNVGII
                     PVSDERLYKIASLVKPQKITPSYLEVLDIAGLVKGASEGKGRGNAFLASIREVDLILH
                     TVRCFEDDNVMHVEGSTDALRDIYIIEDELILKDLETIERREEKLQSQAKSGDKKMKD
                     ALEIVSRLREFIENGNKVRNFPLDEESAKVVREMYLLTRIPVLYVCNVGESDIPDASG
                     NEEVSKVKEYAAKEGAEVIVLSAKIEAEIAELPHDEKKMFLNELGLESSGLDRLAKVA
                     YDKLGLITYFTQGPKEVRAWTVKSGSKAPQAAGVIHSDFERGFIRAETAAYDDFIAAG
                     SEASLKEAGKMRSEGKEYVVKDGDILLFRFNV"
BASE COUNT         1002 a         1328 c         1277 g         1045 t
ORIGIN      
        1 tgtaaatacc ctgggcgcgc gatccgaccg ttaaacgcgg taatatgaaa aatgacaaca
       61 aaattaagag agaaagtacg gcaggcccta tctccctttt ttttctataa gacaccctgt
      121 gcataacgta ctcccggaca atctgattcc atcaccctcg gtatccgcgt aactgcgcgg
      181 cgaataatat tataaaagat caatattagt aactctagtg aattccggtg attttttatc
      241 cccgtaacgc cggctggaag ccccgcaccg aattcggcag ccgtaatcgg gcaaccggta
      301 taaagagtcc ctccgattat atccccgcat ctcgcaaact caccggtttt taggaataat
      361 atcagtttaa tgaaaagctc agaagcgttc ctcctgcgaa agcgccccta cggcgacgcc
      421 gactacgtgc tcacgctctt caccagggaa tacggcaaga taagcgcgtt cgcaaaaaac
      481 gccaaaacaa gcagaaagag gttcggcggg aggcttgagc ccttcgtcct tttccgcgtg
      541 aggttccgcg agaggtcggg agacatgaag ttcgtcgagg acgtggagac gatagaggta
      601 ttcaggcggc tcatggaaga catcgagctc ttcatgtggg gcagcttcct gagcgagtcg
      661 gtcgaaatac ttttgccgaa agaatccccg aacgaggacc tgttcagcct gtttacggat
      721 acgctcaggc agatcgacag cggaaagagc gccatgcccc tcgtgctcgg cttccagctc
      781 ggcgcgcttt cgctctcggg gtacgggccg agcctcgacg cgtgcgctga atgcggcgga
      841 gagaccggaa agggggcccg cttcagcatt aggaaagggg gcgctctctg cgcgggatgc
      901 gcagaggggt tacagaacgg aaccccctac tcgggcgatt tcctaaagga cagggaatcg
      961 atggaaatac aactcggcag ggtgctccgg tacatcaaac tgttctcaaa gttcaccgaa
     1021 taccatactg aaaagaagtt taattcatcc aagttcatag aggagctcaa gatatgataa
     1081 gggtccccgg atttgccgga agcggaatat cgtgcggaat taaaaagagg gggaaaaagg
     1141 acctggccct cctcttctcg gaaaagccgg ccagggccgc gggcgtattt acgacgaaca
     1201 tcgtgaaggc ccccccggtg ctcgttggga tggagcgtat caagagcggg ctctgccagg
     1261 ctgttttagt caacagcggc gtcgcgaacg cgttcacggg caaggccggg atgaagacgg
     1321 ctctgctcac gacggagtgc gcagcggacg agctcggcat cgacaaatca ctcgtcatcc
     1381 cttcctcgac cggagtcatt ggaggccagc tccccgtgga caagatcaac aaggccatgc
     1441 cccatctcgt atcggagctg agagaggacg ggctcgagga cgcggcctcg gccatcatga
     1501 cgaccgatgc gttcccaaag ctcgcgataa cgaaagtggg gataggcggc aggacgggga
     1561 caatatgcgt cataggaaag ggggcgggga tgatcatgcc gaacatggcg acgatgctcg
     1621 cgtacatact taccgacatc aacgtcggaa ggcggacact gacgaaggct ctcggtaacg
     1681 tagtcaataa ttccttcaac aagattatag tggacgggga cacatcgacg aacgatacgg
     1741 tgctgatact ggctaacggt aagctcggga acacggcgtt gggattgacc gggacgcttt
     1801 acagaaaatt cgagcaggcc gtgactgagg ccgcatccga agtggcggag atgatagtga
     1861 aggacgggga gggagcgacg aagactgtaa agataatcgt cataggggca aagtccgtga
     1921 gcgacgcgga gagtgtggcg aggacgatag gcacgtcgct cctcgtgaag agcgcgcttt
     1981 acggagagga ccccaactgg gggaggatag tagccgcggc gggaagggcc ggcgtggatt
     2041 tcgacccgga caggctcgac ctctatatcg gggatcataa ggtactccgg aattcgaagg
     2101 aggtgatgga cgaagccagg gctcttgaaa tcatgaagac gccaaagttc tcaatcacac
     2161 tcgacctcaa gaccgggaag agggcttcgt tcgttataac gagcgatatt acttacgatt
     2221 atctaaagat taatgcgcat tacaggacgt agttaaagtt gaagtacagg ccttggtcag
     2281 gcttacttta gaggctttga gtagttttgg aggcacattt ttagcttccc tcatcgacgc
     2341 cgattagttc atcgagcgat ttgacccctt ccctctccat gtactccacg atcccccggt
     2401 ttatctttcc cgaaacggcc gtgccctcac ccctgatcgc ggtgacgatc tgaagcgccc
     2461 ttgccccggc ccttattttt tccagcgccg tctcggcgtc ctttatcccg cccaccccta
     2521 ttatctcgag cttatccccg gcctgcttgt aaacgtgtgc gactttttcg gtcgtcatcc
     2581 ttctgtaatc ctcgtcgtcg ccgctcaggc cccccgcctg gctcttccat ctctctccgt
     2641 atttggcctt catctctggc acgtcggccg tattggcggc gattatcccg gtgatagagt
     2701 gctcgaccgc gacttcaagt atgtcgtcca cctctttgaa tccgagctcg ggcgcgatct
     2761 tgacgaacag aggcttcctc cctccccggc tttccatggc atcattcacg gcttgtatta
     2821 tatccctctt gcgccccttc tcatggagca tcctgaggcc gggcgtattc ggagagctta
     2881 cgtttatggc gaaatagtcg gcgtaaccgt agagcctctc cgcgacaacg gcgtgcgctc
     2941 tcggggaatc atcgtggggc acgtccctgt tcatgccgat gcttatgcct atcctcacgg
     3001 gcgcgccttc gtatctggag aggttcttcg ccacgacgtc catgcccggg ctgttgaacc
     3061 cgagccagtt aagggcgacg ccgggagaga gcatgaactg cctcggcttc gggttgcccg
     3121 gctgcctgta ctcgagcacc gagcccactt cgacgcccgc gaacccgagc cgccacaatc
     3181 ccttgagggc acggcccgac ttatcccatc ccgcgcctac gatgagaggg ttttcaaact
     3241 cgatccctcc aagagtggtg cggagcctcg ggtcggtaaa cctcttatgc ccgtctgcga
     3301 acagctcgac gaggccgagc gtaagcggcg tcagctccgc cagatgtagt gattcccttg
     3361 caatgttgtg gaaggtctct gaatcgagcc tgtcgaggat cggtcttatg atctctttat
     3421 acattgaacc tgaagaggag tatgtccccg tctttcacga cgtattcctt tccctcggac
     3481 ctcatcttgc ccgcttcctt cagggaggcc tcagagcctg cggcgatgaa atcgtcgtaa
     3541 gccgcggtct cggcccggat aaagccccgc tcgaagtccg agtgtatgac cccggccgcc
     3601 tgcggagcct tcgatcccga tttcaccgtc cacgccctca cttccttcgg cccctgcgtg
     3661 aagtaggtta tcaatccgag tttatcataa gcgaccttcg cgagcctgtc gaggcccgag
     3721 ctttcgaggc cgagctcgtt aaggaacatc ttcttctcgt cgtggggcag ctccgcaatc
     3781 tcggcttcga tcttggcgga aaggactatc acctcagccc cctccttcgc cgcgtattcc
     3841 tttaccttac tgacctcctc gtttcccgag gcgtccggga tgtcgctctc cccgacgttg
     3901 catacgtaga gcaccggtat cctcgtcagg aggtacatct ccctcaccac cttcgcggat
     3961 tcctcgtcga gggggaaatt cctgaccttg ttcccgttct ctatgaattc cctgagacgg
     4021 ctcactatct cgagtgcatc tttcatcttc ttatcaccgc tcttcgcctg cgactggagc
     4081 ttctcctcgc gcctctctat cgtctcgagg tctttcagta taagctcgtc ctctattatg
     4141 tatatgtccc tgagcgcgtc ggtcgagcct tcgacgtgca tcacgttatc gtcttcgaag
     4201 cagcgcaccg tgtggagtat gagatccact tccctgatac tcgcgaggaa ggcgttgccc
     4261 ctccccttcc cttccgaagc gcccttgacg agcccggcga tgtccaggac ctcgaggtag
     4321 ctcggggtga tcttctgcgg ctttacgagg cttgcgatct tgtagagcct ttcgtccgag
     4381 accgggatta tccccacatt atcctcgatc gtcgtaaagg ggaagtttcc catctcggcc
     4441 ccggcgttgg tcagggcatt aaaaagtgtg gattttccta cattgggaag gcccactatt
     4501 ccgcatttta gtcccatagt gtcaatttaa accttttttt gaaatattgt accggcaagg
     4561 aagaaaccgc tgataagcta cctcaacgtg tggcgcagtc aaggtagaag aggcgcacgg
     4621 ttagacgctg ccgggccggg ggaatcaccc tg
//
LOCUS       DASSJH010000178         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1120126, whole genome shotgun sequence.
ACCESSION   DASSJH010000178 DASSJH010000000
VERSION     DASSJH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120126"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VFJ67_03820"
     CDS             complement(<1..1087)
                     /locus_tag="VFJ67_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7289543.1"
                     /translation="MKEQRVSAQMGDILIKEDVINLDQLKTAIQEQKETGKRLGETLL
                     NLGYIDEHQLVAYLSKQYGVPAINLDQFDISIDVLKTVSRESALKHKLIPINKSGSTL
                     VVAMSDPSNIFAVDDLKFATGHNIEVVVTSERSIRNAIDRFYGSQQEWEDARKAQENT
                     AAVDKLMGDLDEFLIEISGEENIDLNDLEKASEEAPVIKLVNHLLMDSIRKGASDIHI
                     EPYEKSLRIRYRIDGVLYDIMHPPLKLKNAVTSRIKVMSNLDIAERRLPQDGRIKVKM
                     AQNRTMEYRVSVVPTLFGEKVVMRILDKESLQLDLTKLGFYEEQLEVFRRSIYQPFGM
                     VLITGPTGSGKTTTLYSSLMDLNQIDVN"
     gene            1713..>2302
                     /gene="trmFO"
                     /locus_tag="VFJ67_03825"
     CDS             1713..>2302
                     /gene="trmFO"
                     /locus_tag="VFJ67_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET7289544.1"
                     /translation="MKSGKEIIIAGGGLAGSEAAHAAAKAGARVRLYEMRPVKPSLAH
                     NTGDLGELVCSNSLKSDSLDNASGILKEEMRYLGSLVLEAAEKTRVPAGKALAVDREA
                     FAQYITERIENNPLIELVREEFDSIPDSPDAPLIIATGPLTSEPLAQNIRELTESDHL
                     YFYDAISPIIDGDSIDYSRVYRASRYEDGETGEGDYL"
BASE COUNT          546 a          588 c          561 g          607 t
ORIGIN      
        1 tattcacgtc tatctggttc aggtccatga ggctcgagta aagggtcgtc gtcttaccgc
       61 tccccgtcgg tcctgtgatc agcaccattc cgaatggctg gtatatggac ctcctgaaga
      121 cctcgagctg ttcctcgtaa aacccgagct tcgtcaggtc gagctggaga gactccttgt
      181 ccagtatcct catcacgacc ttctctccga atagcgtcgg cactacggaa acacggtatt
      241 ccatcgtcct gttctgggcc atcttcacct tgatgcggcc gtcctgcggg agcctccttt
      301 cggctatgtc gaggttagac attaccttta tcctcgacgt tacggcgttc ttgagcttca
      361 acggcgggtg cattatatcg tagagcacgc cgtctatcct gtatctgatt cgtaacgatt
      421 tttcgtaggg ctctatgtgt atgtcgctcg cgcctttccg gatcgaatcc atcagcaggt
      481 gattcacgag ctttattacc ggagcttctt ctgacgcttt ctccaagtca ttgaggtcga
      541 tgttctcttc gcccgatatt tcgatgagga attcgtccag gtcgcccatg agcttgtcaa
      601 cggcggccgt gttttcctga gccttccttg cgtcctccca ttcctgctgc gagccgtaga
      661 atctgtcgat cgcgttcctg atcgacctct cggacgtcac gacgacctct atgttgtgac
      721 cggttgcgaa cttcaggtcg tcgacggcga atatattgga cgggtcggac atcgcgacga
      781 cgagcgtcga gcccgatttg tttatcggga tgagtttatg ttttaatgcg gactctctgg
      841 atacagtctt gagaacgtcg atcgagatat cgaactggtc gagattgatt gccgggacac
      901 cgtattgctt gctcagatac gcgacaagct ggtgctcgtc gatgtagccg aggttgagga
      961 gggtctcgcc gagcctcttc ccggtttcct tctgctcctg gatggcggtt ttaagctgat
     1021 caaggtttat tacgtcttcc ttgataagaa tgtcgcccat ctgagcgctg actctctgtt
     1081 ctttcatatc tctattaccg tcacatgggg gtcttattaa attacgatct acgacccgcc
     1141 cgcggatgga ttatttattc aaagcaagtg ctgagtgttg aaaatagaca aatggattaa
     1201 cagcgatcca agtgccgatg atcacaattc cccgcccctc aagagaaaat ttaacgcaat
     1261 agctcttccc gggcttatat atattcgccc atatccggcc gacgcttcac cccagtctat
     1321 ttctatgccc agaaccactt tataatttta atcctctttc gagttaatcc gaccactacg
     1381 gataaaatag taaaaatagc aatatatatg ccagagagcc ggtctgtgca ggcttgacca
     1441 taagttacta cgattacaat aaattcttaa gaattccgtg taacccgcag ttatcactga
     1501 tgaattaacg tgacgaatat tgtatcgcta agactttatt tgtcacaaat ggcatataac
     1561 taaccggcag caggaaagtt aaataattta taacgaaagc aagtttggga tatttaaaag
     1621 gtactgctca attcctacta ctgctctgtg ttttccttaa agaccgagct caactcacct
     1681 gatattccgg ccgttcgggt aatataaagc gcatgaaatc cgggaaagaa ataatcatag
     1741 cgggcggagg actcgcgggc tcggaggccg cccatgcggc agcgaaagcg ggcgcgaggg
     1801 tcagactcta cgagatgaga cccgttaaac cgtccctggc gcataatacc ggcgacctgg
     1861 gagagctcgt ctgcagcaac tcgcttaaat cggattccct cgataacgcg agcggtatat
     1921 tgaaagagga aatgagatac ctgggctcgc tcgtactcga agcggccgag aaaacccggg
     1981 ttcccgcggg taaagccctc gctgtcgaca gggaagcttt cgcacaatac ataacggaga
     2041 ggatcgagaa taatcctctt attgaattag taagggagga gttcgatagt atcccggatt
     2101 ctcctgacgc acctctaata atcgcgaccg gccccctcac ttcggagccg ctcgctcaaa
     2161 atatccgcga gctgacagaa tccgatcatc tctatttcta cgacgcgata tccccgataa
     2221 tagatggtga ctcgatagat tattcgaggg tatacagggc atcgaggtat gaagacgggg
     2281 agacaggcga gggcgattac ct
//
LOCUS       DASSJH010000179         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_42158, whole genome shotgun sequence.
ACCESSION   DASSJH010000179 DASSJH010000000
VERSION     DASSJH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42158"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..1965)
                     /locus_tag="VFJ67_03830"
     CDS             complement(361..1965)
                     /locus_tag="VFJ67_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7289545.1"
                     /translation="MSTLYRYLKWDGRQPEFGLDSGDFFSEMSDYLMEGWTPDEAYEW
                     ILKQGLKGQKTKVMGIDGLRSELASWRQKAYEKYNPGTALDRIKSELDEIINRELSHI
                     KNTLPADSHELGERERFLGSLDPKPVRAVESLSGYEFLDGEAGRRFRALLERLEDMRK
                     AERFIKRFGEKFTGETPVDLDGLLELIDRLEELDSIEKSLISGDFDDIDTDGISWALS
                     GEAGESLYLLKNFKSALEKSGYIRSSGERTVLTPRGIRKLGESALKDIFSSLNRDKIA
                     EHDTGLRGSGPAKHEESKDYEFGDPFNLNIVNTLKRSLARESEGRGIKIDPGDFEVYE
                     TEYQTQAATALLLDLSWSMSFQGRFPAAKRVALALDHLIRTKYPKDDFHIIGFSTGAR
                     KLTTRELAVTTWDSNDPFTNIQEALTVAARTISGSRSANKQIILITDGQPTAYYLDGY
                     LQVELPMFFGGLSPRATFETLKEVKRVTGMGIRINTFMLDDSPSLRRFVDEMTRINKG
                     RAFFTTPDQLGKYLFVDYMKRKRKVL"
     gene            complement(1962..3359)
                     /locus_tag="VFJ67_03835"
     CDS             complement(1962..3359)
                     /locus_tag="VFJ67_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HET7289546.1"
                     /translation="MQGRPATIGELKASEYRVLSVKDEMRKNLIARLRRGENPFTGII
                     GYEDTVIPQVETAILAGHDIIFIGERGQAKSRLIRSLVNLLDDEVPVIKGGELNDNPY
                     SPVTKLSRELVAKYGDDTEIEWLGRDGRYSEKLATPDVSVADLIGEVDPIKVAEGRYL
                     SDELTIHFGLIPRTNRGIFAINELPDLVEKVQVGLFNVMEEKDIQIKGYKIRLPLDIC
                     IVATANPEDYTNRGRIITPLKDRFQSQISTHYPKTRDVEIVIMEQESYVPDIEGFSLR
                     VPKFIKEIIAELSFQARSSGDINQRSGVSVRTTIANYESIIAAAEKRGITIGEREVAP
                     RITDFPAIIPSTSGKIELEYLGEDSSEEAVVEKLIKRSIKAVFDSYFPALDVLADVIK
                     SFEQGYVEVADVMPSEDYIMGLREIKGLRGCIDSLGISDSPAEIAAAIEFTLEGLHLN
                     NKLNKENVRGKIIYK"
BASE COUNT          726 a         1111 c          976 g          983 t
ORIGIN      
        1 ctatgattcc aatgtatctc ctccgttcct tccaacaaat gatgccatcc gttcatcctg
       61 cttgcccggc gaagtcttga cgaagccggg aagccggaag cgacgcggca atcccgcctt
      121 tttctttccc gacatcacat aatttacgtt aagaaaatat agagtattaa ggcgtgaaaa
      181 atgttttagg gttgcggtag aattaaaaca ttttcgcctt aatacgatat ttttaggaaa
      241 ttctgtgctg tcggttgatt tttgcttctt ttcatcaagg aaaagaagga taaaaaagaa
      301 aagaaggata caaaattcat ttcaccgttt aatttcaatt cgtcatatgc acaacggatg
      361 tcaaagcacc ttcctcttcc tcttcatata atcgacgaag aggtatttac ccagctggtc
      421 gggcgtggtg aagaacgcgc ggcccttgtt gatccgggtc atctcgtcga cgaaccgcct
      481 gagcgacggg ctgtcgtcca gcatgaacgt gtttatgcgt atccccatcc cggtcacgcg
      541 cttcacttcc ttgagcgtct cgaacgtggc cctcggcgag agccctccga agaacatggg
      601 caattcgacc tgtaaatatc cgtcgaggta ataagccgtg ggctggccgt ccgtgatgag
      661 aattatctgc ttgttggcgc tcctcgagcc agatatagtc ctggcggcga ccgtgagcgc
      721 ctcctgtatg ttcgtgaacg ggtcgttcga atcccaggtc gtgacggcaa gctccctcgt
      781 cgtcagcttc ctcgcccccg tggagaagcc tataatgtga aagtcgtcct tcggatattt
      841 cgtccttatc aggtggtcga gcgcgagcgc gactctcttc gcggccggga acctgccctg
      901 aaaactcatc gaccagctta ggtcgagaag gagcgccgtc gccgcctgcg tctggtattc
      961 cgtttcataa acctcgaaat cccctgggtc gatcttgata ccccggcctt cggattccct
     1021 cgcgagcgac cgcttgagcg tgttcactat gttcaggttg aacgggtccc cgaactcgta
     1081 gtctttggat tcctcgtgct tcgccggccc cgagcccctg agcccagtat cgtgctccgc
     1141 tatcttgtcc cggttgaggg aggagaatat gtccttgaga gccgattccc cgagtttcct
     1201 tatccccctc ggtgtgagca cagtacgctc gccgcttgac cttatgtatc ccgacttctc
     1261 gagcgcagac ttgaagttct tgagtaggta gagcgattcg cccgcttccc cgctcagagc
     1321 ccagcttatc ccgtccgtgt ctatgtcgtc gaagtccccc gagatgaggc tcttttcgat
     1381 gctgtccagc tcttcgagcc tgtctatgag ctcgaggaga ccgtctaaat cgacgggtgt
     1441 ttcgcccgtg aacttctcgc cgaaacgttt aatgaacctc tcggctttcc tcatgtcctc
     1501 gagcctttcg agaagagcgc ggaacctccg tcccgcctcc ccgtcaagga attcgtaccc
     1561 gctcaggctc tcgaccgccc gcacaggctt cgggtcgagc gagcccagga acctctccct
     1621 ctcgccgagc tcgtgggaat cggcgggcag ggtgtttttt atgtgagaga gttctcggtt
     1681 aataatttcg tccagctccg attttatccg gtcgagtgcc gttccgggat tgtatttctc
     1741 gtaggccttc tgcctccacg aagcgagctc cgagcggagg ccgtcaatac ccatgacctt
     1801 cgttttctgc cctttgagac cctgcttgag tatccactcg taggcctcgt caggggtcca
     1861 gccctccatg agataatcgg acatctcgct gaagaagtcc ccggagtcga gaccgaactc
     1921 gggctgcctc ccgtcccatt tgagatacct gtaaagtgtg ctcatttgta aattatcttt
     1981 ccccggacgt tttccttgtt cagcttgtta ttcaggtgga gcccttcgag cgtaaattcg
     2041 atcgccgccg ctatctccgc gggcgagtcc gagatcccta gcgaatctat gcagccccga
     2101 agacccttta tctcccggag ccccatgata tagtcctcgg acggcatcac gtccgcgacc
     2161 tccacgtagc cctgctcgaa gctctttatc acgtccgcga gcacgtccag ggcagggaag
     2221 taggaatcga atacagcttt tatcgagcgc tttatgagct tctcgactac ggcctcttcc
     2281 gaggagtcct cgcccaggta ttcgagctct atctttccgc tcgtcgatgg aattatggca
     2341 gggaagtccg ttatgcgggg cgcgacctcg cgctccccga tcgttatacc ccttttttca
     2401 gctgccgcta ttatgctttc gtagttggcg atagtggtcc tgacgctcac gcccgagcgc
     2461 tggttgatgt cccccgagct gcgcgcctga aacgagagct cggcgattat ctcttttatg
     2521 aacttcggca ccctgagcga gaacccctct atgtcgggga cgtaggactc ctgctccatg
     2581 atgactatct ccacgtcgcg cgtcttcgga taatgcgtgc ttatctggga ctggaacctg
     2641 tccttgagcg gggtgattat tctccccctg ttcgtatagt cctccgggtt ggcggtggcc
     2701 actatgcaga tatcgagagg gagccttatc ttgtacccct ttatctgtat atccttctct
     2761 tccatcacgt tgaagagccc cacctgcacc ttctccacca ggtccgggag ctcgtttatg
     2821 gcgaatatgc ccctgttggt cctcggtatg agtccgaaat gtatcgtgag ctcgtcggac
     2881 agatacctgc cttcggccac ctttatcgga tctacttcgc ctataaggtc ggcgaccgag
     2941 acatccggcg tggcgagctt ttcgctgtac ctcccgtccc tcccgagcca ttcaatctcc
     3001 gtatcgtccc cgtatttcgc gacgagctcg cgtgagagct tcgtcacggg cgagtagggg
     3061 ttgtcgttca gctctccccc ttttatgacc gggacctcgt cgtcgagcag attcacgagg
     3121 gaccttataa ggcgcgactt cgcctgtccc ctttcgccga tgaatatgat gtcgtggcca
     3181 gcgagtatag ctgtctcgac ctggggaatg actgtgtctt cgtagcctat tatccccgtg
     3241 aacgggttct ctcctcttct aagtctcgcg atgaggtttt tcctcatctc gtcttttacg
     3301 gagagcaccc tgtactcgct cgctttgagt tccccgatag tcgcgggtct gccttgcatt
     3361 atgtttcccc ctcgggacag atttaatcgg ctgcaaatct cgcggggcgc gggctgtcgg
     3421 atgatccggc ccagagcctc cgtgataagc ggctgccgga aaaatcacgc gataaccgga
     3481 aacggcagcc ctcggacggt ttgctcgaaa ttatcaccgc ttacccgtta aaattataat
     3541 ggttatcggg caggatttga agcccgattg tcaacacgat gcccccggcg gggtttcaat
     3601 agggtcccct ccggtatata attgatgaca ggaccgtggt gaaaaacggg actttgagtc
     3661 gtatttgccc gtttttcatc ataagtatca gggagggagc ggcatccgtc cggatcgcgc
     3721 gccggcggct tctttttaag ccgtcgcaac cgcgccgaat gtcaccgccc cgcaaaaact
     3781 gtccgacgga gaaaag
//
LOCUS       DASSJH010000180         5352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_367354, whole genome shotgun sequence.
ACCESSION   DASSJH010000180 DASSJH010000000
VERSION     DASSJH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367354"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFJ67_03840"
     CDS             complement(<1..534)
                     /locus_tag="VFJ67_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HET7289547.1"
                     /translation="MSERVVDNILDLIGGTPIIKLNSVVPEGSADVWAKLENLNPAGS
                     VKERICLRMIATAEESGAIEPGKSVIVEPTSGNTGIGLALVCAVKGYRLILTMPDDMS
                     LERRQLLTAYGAEVVLTPASEQMEGAVRKAEEILKEHPFSFMPQQFGNMENPSAHREG
                     TGPEILEQMGGRIDAFVA"
     gene            complement(598..795)
                     /locus_tag="VFJ67_03845"
     CDS             complement(598..795)
                     /locus_tag="VFJ67_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289548.1"
                     /translation="MEESEETLDSYLKKMEDQELKGLLLKLRNELRKPDASWESVRSV
                     LESVKRKNSRVFSEVATLIVS"
     gene            910..3609
                     /locus_tag="VFJ67_03850"
     CDS             910..3609
                     /locus_tag="VFJ67_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET7289549.1"
                     /translation="MARKKKVKLKSKPKKSKFLAFLLVAFLFLSASGILALYGAYLYY
                     SRDLPNFRAITGYKPKLITEVYSSDGTLIAEFAAERRKLIPYEEIPPRVRDAFIAVED
                     RRFFEHEGVDIKSILGAVLENIQEGDWVRGASTITQQVIKNIILTPERTLSRKIKEAI
                     LAHRIENNLSKEEILYLYLNHIYLADGTYGVEAASWNYFGKSARDLTLGEAAVLAGLP
                     KKPEYYSPRKHLDRALERQKLVLSKMEEAGFITRDQRFDALDQKITIVPKKRVNNDLA
                     PYFVEHVRRYLQDKVGVKDFLNGGYTVFTTLDVDLNLEAQWALKRGIMSLDARHGHNF
                     VSRHLASDKEIKRFTREQGVKSVEIGGTYDAVITGVTKDDKSKDSDKPLYTASVRIGD
                     VAGVLKFAVSSPFGTAVPGLKTPYPGKLAPADGYEGTSLSPVELKTGDVVKVEADDTQ
                     DGKYYFSLLYIPAAQAALISMESNGRIKALVGGYDFGLSQFNRTTQAMRQPGSSFKPI
                     VYSAAVDKGYSETSILYDMPVVVKDWAPQNYDGNYEGAMVLRRALAKSRNLASIRLII
                     DIDPKYAQKYATSNFGFTSKLQPYPSLVLGGSDVTVLEMADAFNVFATGGNLVEPQFI
                     LRIYDRNGKIIEDNTGGKFTSKEEALKAEREDARQKILDELARKKGVEPLESPEYISE
                     EVLTEKSDVSANADKYTFLTPGEFLELLREKSVDFTSAGNSRQTLKPETAFIMTDLLQ
                     AVVKEGTGMRALKLTSLAPIAGKTGTSNDYTDAWFVGFSPKITTAVWVGRDDHKPLGR
                     KEAGSAAALPIWIDYMEDALAKFPGGVFKQPAGIKMVSTPYGYIPYSLDSLRDNVIDS
                     IRDRVMIDGQAVDNTGMYYPKELGGVRNGQPETETEIDYLLKR"
     gene            complement(3606..4130)
                     /locus_tag="VFJ67_03855"
     CDS             complement(3606..4130)
                     /locus_tag="VFJ67_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7289550.1"
                     /translation="MAIRNASKKTSNSLLYLALLCLLIYLVKTHNDYSSPSVVEGGDI
                     TGKIYVEIDDGTFPTVRAISKPEELAEIRHLYNIDSELKNGSKVILYDGKEARTGRIS
                     GLKSLSLGIPIGINSASAEGLEALPGIGEKLALRIVAYRNQAGGFKDADQLLNVNGMG
                     EKKLALVKPIINLD"
     gene            complement(4222..5013)
                     /locus_tag="VFJ67_03860"
     CDS             complement(4222..5013)
                     /locus_tag="VFJ67_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7289551.1"
                     /translation="MDLGIKDKVAIVGASSKGLGKAVALGLAREGVKLTLCARGKKDL
                     DKTADEIVKETGAEVLAIECDMSKTDDIKKVVDETIKKYLRIDIVVNNAGGPPTGAFL
                     DFSLEDWQKAIELNLISTITLSKAALPCMKERKWGRIINITSVAVKQPIDGLILSNTA
                     RAGVIGLAKTLSNEFGPYNITVNNVCPGRILTDRITHLASEKAKREGTSLEDALASME
                     QDLPARRIGRPEELADLVVFLASERASYITGTTIQVDGGLTKGLF"
     gene            complement(5110..>5352)
                     /locus_tag="VFJ67_03865"
     CDS             complement(5110..>5352)
                     /locus_tag="VFJ67_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289552.1"
                     /translation="ISLSSFQLTGTGWYSTDYAKGPGIPPHANDLGTAPKDGTEAKDK
                     KETPGLPTEAAAVSGTSTYDSIKKDTAKAKSKSKGS"
BASE COUNT         1179 a         1499 c         1455 g         1219 t
ORIGIN      
        1 ggccacgaac gcgtctatcc tgccgcccat ctgctcgagt atttcggggc ccgttccttc
       61 cctgtgcgcg gacgggttct ccatgttgcc gaactgctgg ggcatgaagg agaaggggtg
      121 ctccttcaat atctcctccg ctttcctgac cgctccttcc atctgctcgg aggcgggcgt
      181 gaggacgacc tccgcgccgt acgctgtcag aagctgcctc cgctcgaggc tcatgtcatc
      241 gggcatcgtg aggatgagcc tgtatccctt gacggcgcac accagagcga gcccgatccc
      301 ggtgttcccg ctcgtgggct cgactatcac gctctttccc ggctctattg ctcccgattc
      361 ttccgccgtg gcgatcatcc tgaggcatat cctttccttg acgctcccgg cggggttcag
      421 gttctcgagc ttcgcccaca cgtcggccga gccttccggg accacgctgt tcaatttgat
      481 tatcggcgtc ccgccgataa gatcaagaat gttgtcgact actctctcgc tcatttgatc
      541 cgggttgccg cggtcaggta ttgttacggc gctgcggccc gcgccttcat tttattatca
      601 gcttactatc agcgtcgcga cttcactgaa tacgcggctg ttttttctct tcaccgattc
      661 caggaccgac ctcacgctct cccaggacgc gtcgggcttc ctgagctcgt tcctgagctt
      721 gagcagcagg ccctttaact cctggtcttc catttttttc aagtaggaat cgagggtctc
      781 ttcgctttct tccatcggtc cgccctgcct gaaatttgat ttatttaata atcccgactc
      841 aggtataatt aaccttgaat cccggaaagt caatcattta tacgctatta aaggattaag
      901 tacagcttaa tggccaggaa aaagaaggtt aaacttaaat caaaacctaa aaaaagcaaa
      961 ttcctcgcct tcctcctagt tgcgtttctt ttcctttcgg catccgggat actggcgctc
     1021 tacggggcgt acctctacta ctcgcgcgac ctccccaact tcagggctat cactggctac
     1081 aagcccaagc ttataaccga ggtctattcc tcggacggca cgcttatcgc cgagttcgcc
     1141 gccgaaagac gcaagctcat tccttatgag gaaatacctc cccgcgtgag ggacgcgttc
     1201 atcgccgtcg aggacaggcg ctttttcgag cacgagggcg tcgacataaa aagcatactg
     1261 ggcgcggtgc tcgaaaacat acaggaaggc gattgggtta ggggcgcgag caccataacg
     1321 cagcaggtga taaagaacat tatcctcact cccgaaagga cgctttcgag aaagataaag
     1381 gaagccatac tcgcgcacag gatagagaac aacctctcca aggaagaaat actgtatctc
     1441 tacctcaacc acatctatct cgcggacggc acgtacgggg tggaggcggc gagctggaac
     1501 tatttcggta aatccgcgag ggacctgacc ctcggcgagg ccgcggttct ggcggggctt
     1561 cccaagaagc ccgaatatta ctctccgagg aaacacctgg acagggcgct cgagagacag
     1621 aagctcgtcc tcagcaagat ggaggaagcg ggtttcatca cgagggacca gaggttcgac
     1681 gctctcgacc agaaaataac gatagtcccg aagaaaagag tgaataacga cctcgcgccc
     1741 tatttcgtcg agcacgtgag gaggtatctc caggacaagg tcggggtgaa ggactttctc
     1801 aacggcggat acaccgtctt caccacgctc gacgtggacc tcaacttaga ggcgcagtgg
     1861 gcgctcaaaa ggggtattat gagcctcgac gcgagacacg gccacaattt cgtctcgagg
     1921 caccttgcaa gcgataagga gataaaaaga ttcacccgtg agcagggcgt aaaatccgtc
     1981 gagatcggcg ggacgtacga cgccgtgata acgggggtaa cgaaagacga caaatccaag
     2041 gattccgata agcctttata caccgcttcc gtacggatag gggacgtggc cggcgtgctt
     2101 aaattcgccg tgagctctcc tttcggtacg gccgtacccg gtctcaaaac cccttatccg
     2161 ggcaagctcg cccccgcaga cggatacgaa ggcacgagcc tctctccggt cgagctcaag
     2221 acgggagacg tcgtaaaggt cgaggccgac gatactcagg acggcaagta ctatttctcg
     2281 ctcctctaca taccggcggc tcaggccgcg ctcatctcga tggaatccaa cggcaggata
     2341 aaggccctcg taggagggta cgacttcggt ctctcccagt tcaacaggac cactcaggct
     2401 atgcgtcagc ccggctcttc gttcaagcct atcgtttatt ctgcggccgt ggacaaaggg
     2461 tacagcgaga cctcgatatt gtacgacatg cccgtcgtgg tcaaggactg ggccccccag
     2521 aactacgacg gcaactacga aggggctatg gtcctgagga gggcgcttgc gaaatcgcgc
     2581 aacctcgcga gcatcaggct catcatcgac atcgacccca aatatgccca gaagtacgcg
     2641 acgagtaatt tcggtttcac atccaagctt cagccgtacc cgtctcttgt cctgggaggc
     2701 tccgacgtga cggtgctcga aatggcggac gcgttcaacg tattcgctac gggcggaaat
     2761 ctcgtcgagc cacagttcat tctccgaata tacgacagga acggaaagat catcgaggac
     2821 aacacgggcg gaaaattcac ctcgaaggag gaagcgctca aggcggagag agaggacgcg
     2881 aggcagaaga tactggacga gctcgcccgg aaaaagggcg tggagccgct cgaatcgccc
     2941 gagtacatat cggaagaagt gctgaccgaa aagagcgatg tatccgcgaa cgccgacaaa
     3001 tacactttcc tcacgcccgg ggaattcctg gagctgctga gagagaagtc ggtcgacttc
     3061 acgtcggcgg gtaattccag acagacgctc aagccggaga ccgctttcat aatgaccgac
     3121 ctccttcagg cggtcgttaa ggaagggacc ggaatgaggg cgctcaagct cacgtctctc
     3181 gctccgatag ccggcaagac gggaacgtcg aacgactaca cggacgcgtg gttcgtaggg
     3241 ttcagtccga aaataactac tgcggtatgg gtcgggaggg acgaccacaa gccgctcgga
     3301 aggaaggaag cgggctcggc ggctgcgctc ccgatatgga tagactacat ggaagatgcg
     3361 ctcgctaaat tcccgggagg cgttttcaag cagccggcgg gcataaaaat ggtctcgaca
     3421 ccatacggat acatacccta cagcctcgat tcgctcagag acaacgtgat agattccata
     3481 cgcgacaggg ttatgatcga cggccaggcg gtcgacaata ccggaatgta ttaccccaag
     3541 gaattaggcg gggttagaaa cggccagccc gaaacagaga ccgagataga ttaccttctc
     3601 aagcgttagt cgaggttgat gatcggcttc acgagtgcaa gcttcttctc gcccattccg
     3661 tttacattaa gcagttggtc ggcatcttta aatccgccgg cctgattcct gtaggccact
     3721 atacggagag cgagcttttc gcctattccc ggaagcgctt cgaggccttc cgcgctcgcg
     3781 gagtttattc ctatagggat accgagagag aggctcttca acccgctgat cctgcccgtc
     3841 cgggcctctt tgccgtcata aagtataact ttagaaccgt tctttaactc gctatcaata
     3901 ttgtataaat gtctgatttc cgcgagttct tcaggctttg agatggctct gaccgtcggg
     3961 aatgtcccgt cgtcgatctc gacgtagatc tttccggtta tatcgccgcc ctctacaacg
     4021 ctcggggatg aataatcgtt atgggttttc acgagatata tgagtagaca caatagggca
     4081 agatacagga gggagttgga agttttttta gaagcgttgc gaatcgccat ttattcattc
     4141 cccgatggtc aaagcgtgga aggaaattaa ctcccagctc aacgttgcct aagccaaaat
     4201 aggccaggga ggattttttc tttaaaaaag cccctttgtc agcccgccgt ccacctgtat
     4261 ggtggtcccc gtgatataac tcgcccgctc cgaggccagg aacacgacga ggtcggctag
     4321 ctcctcgggt cttcctatgc gtcttgccgg caagtcctgc tccatcgagg cgagagcgtc
     4381 ttcaaggctc gttccttctc ttttcgcttt ttccgaagcc agatgcgtta tcctgtccgt
     4441 gagtatcctt cccggacaca cgttattcac ggtgatgtta taggggccga attcgttgga
     4501 cagggtcttc gcgaggccga taactccggc cctcgccgtg ttcgagagta tgagcccgtc
     4561 aatgggctgc ttcacggcga cggacgtgat gttgattatg cgtccccatt tgcgctcctt
     4621 catgcagggc agggcggcct tgctcagggt gatggtgctg atgagattga gctcgatcgc
     4681 cttctgccag tcctcgagcg aaaagtcgag gaacgccccc gtgggagggc cgccggcgtt
     4741 attcacgaca atgtcgatcc gcaaatattt ttttattgtc tcgtcgacga ccttttttat
     4801 gtcgtccgtt ttcgacatat cacactcgat tgcgagcact tcggcgccgg tctcctttac
     4861 gatctcgtcc gccgttttat cgaggtcttt ctttcctctc gcgcataaag tgagctttac
     4921 tccctcccgc gcaaggccga gagcgaccgc tttgccgagg cccttgctgg atgcgcctac
     4981 gatagcgact ttatctttta ttccgaggtc catgatattt tgtggttatc cttttcgagc
     5041 gcaccgtccg agtaaataaa agggacgcgg agcagggcag ggggggcgaa gtccggcctt
     5101 ctcatccttt taagagcctt tggatttgga cttggctttt gccgtgtctt tctttatcga
     5161 atcgtacgtc gatgtgcccg atacggctgc ggcttcagtc gggagtcccg gagtttcttt
     5221 cttgtccttg gcctccgtgc cgtctttcgg ggccgtgccc aggtcgttcg catggggcgg
     5281 aatgcccggg cccttggcgt aatccgtcga ataccatccg gtccccgtga gctggaagga
     5341 cgagagagat at
//
LOCUS       DASSJH010000181         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_475575, whole genome shotgun sequence.
ACCESSION   DASSJH010000181 DASSJH010000000
VERSION     DASSJH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475575"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..1417)
                     /locus_tag="VFJ67_03870"
     CDS             complement(332..1417)
                     /locus_tag="VFJ67_03870"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET7289553.1"
                     /translation="MAKAKVKEGTDSVVRLLGKEADYLLDHKCKTVTKNLLHLPGPDF
                     VERVFAPSDRSNSVLRNLQLMYNTGRLAGTGYLSILPVDQGIEHSAGASFAPNPIYFD
                     PENIVKLAIEGGCNAVASTLGVLGAVSRKYAHKIPFLVKLNHNELLTYPNKYDQIMFA
                     NVDQAFQMGAVAVGATIYFGSEESDRQIQEVSEAFSYAHTLGLVTVLWAYLRNPAFST
                     AKVDYHLSADLTGQANHISATIEADIVKQKQAENNGGFLALKFGKTDPKVYSKLTSDH
                     PIDLTRYQVVNCYMGRAGLINSGGASTGREEDDLAQAVRTAVINKRAGGMGLITGRKS
                     FQKPMKDGVEILNAVQNVYLDESITVA"
     gene            complement(1535..2755)
                     /locus_tag="VFJ67_03875"
     CDS             complement(1535..2755)
                     /locus_tag="VFJ67_03875"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7289554.1"
                     /translation="MDKLVISDLPDSVFHGKRVFVRVDFNVPIRDGKISEDYRIRRAL
                     PTIDYLVERKAKVILGSHLGRPKGIAIADLSLKPIAMRLSDLLGKSVKFNGKVIGPEV
                     RDALDALEPGEVMLLENLRFHKEETDNDPAFSKELASYADIYVNDAFGTSHRKHASTY
                     GMASMFDLRVAGFLVSRELKFLTRLRENPDRPFLVIVGGSKIKDKIHALKSLIEKADR
                     VLLGGGAAYTFLKAKGVNIGSSIVEDEFVGWAKEALDKYGDKIFLPVDHVVAGNLEGR
                     KNCMVVDGEIPDDLKGYDIGPKTAAKYNHLIKGTGSIFWSGPMGVFEIGDFSAGTTQL
                     ARSVALATWRGATTVVGGGETIAAIREAEVLDLEITHISTGGGALLEFLGGDELPGIS
                     ILNDKEEKISMAAS"
     gene            complement(2755..>3079)
                     /locus_tag="VFJ67_03880"
     CDS             complement(2755..>3079)
                     /locus_tag="VFJ67_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7289555.1"
                     /translation="SAVAMRVPTADVSVVDFTCEVKKHTSPEEVNEKFRQAASGELKG
                     YLRYSDEELVSSDFIGDPHSAVFDSALTSVVDGNLVKVIGWYDNEYGYSSRVVDLLEF
                     IGEKM"
BASE COUNT          626 a          905 c          769 g          779 t
ORIGIN      
        1 cccctatgcc gcccggcctt ataacgagaa tcctgcggct ctccctatcc ccgtattttc
       61 gggggccgac cataagtttg gataccgagc aagagatact tccgagcacc gcgtccagtt
      121 tcttcagtcc ttccatcttc cagttcatta cggttatccg gtcataaagc ggctcacgtc
      181 aggctagtac acgacccgcc gactctagta taaatcttcc gggataatat aggtaggatg
      241 aagttggtct tcaggctcgg aagcggatat cgaatacggg ccgaggtctt aacggcttta
      301 gctcttatta cggaaaacag cagcacggtt atcaggctac ggttatagac tcgtccaggt
      361 atacgttctg tacggcgttc agtatctcga cgccatcctt catcggcttc tggaaagact
      421 tccgccccgt gatgagcccc atgccgcccg ccctcttgtt gatgacggcc gtccttacgg
      481 cctgtgcgag gtcgtcctct tccctgcccg tagaagcgcc tccggaattt atcagtcccg
      541 cgcggcccat gtagcagttc actacctggt acctggtcag gtctatcgga tgatcggacg
      601 tgagcttcga gtagaccttc gggtcggtct ttccaaattt gagcgcgagg aaccccccgt
      661 tattctccgc ctgcttctgt tttacgatgt cggcttctat ggttgcggaa atgtggttcg
      721 cctgccccgt gaggtcagcc gacaaatgat aatccacctt tgccgtgctg aatgcagggt
      781 tcctgagata agcccagagc acggttacga gccccaatgt gtgcgcgtag ctgaacgcct
      841 ccgagacctc ctgaatctgc ctgtcgctct cctctgagcc gaagtatatc gtagccccga
      901 cggcgaccgc tcccatctgg aatgcctgat ccacgttcgc gaacattatc tggtcgtatt
      961 tgttagggta agtaagcagc tcgttgtgat tgagctttac gaggaacggt atcttgtgag
     1021 catatttcct cgaaacagcg cccaacactc cgagagtgga ggcgacggcg ttgcagcctc
     1081 cttcgattgc gagcttcacg atattttccg gatcgaaata aatgggattc ggagcgaaag
     1141 atgcccccgc agaatgctct atgccctgat cgaccgggag aatcgacaga taacccgtgc
     1201 ccgccagcct gcccgtgtta tacatgagct gaaggttacg cagcacagaa ttcgacctgt
     1261 ccgaaggagc gaaaaccctc tcgacaaaat cggggccggg cagatggagt aaatttttgg
     1321 taaccgtttt gcacttgtgg tcgagcagat aatccgcctc tttacccaag agcctgacta
     1381 cggaatccgt cccttctttc acttttgcct tcgccatttt tatgctcctt atttgattcc
     1441 agaaccgtgt cctaaagata acacgaaatt atttggatgg gtagattcag aacgccgtga
     1501 tcgggcacgg aatttcattt tacggatacg gattttaact cgcggccatg gaaatctttt
     1561 cttctttgtc gttgagaatc gagattcccg gaagctcgtc gccgcccaga aattcaagaa
     1621 gcgcgcctcc gccggtcgag atgtgagtga tctcgagatc aagcacctcc gcctccctta
     1681 tcgcggcaat ggtctcgccg ccgccgacaa ctgtcgtagc ccccctccac gtcgcgagcg
     1741 ctacggacct cgcgagctgc gtggtgccgg ccgaaaaatc gccgatctcg aaaaccccca
     1801 tgggcccgct ccaaaagatc gatccggtcc cttttatcag gtggttgtac ttggcagccg
     1861 tcttgggtcc tatgtcgtag cccttgaggt cgtccggtat ctcgccgtca acgaccatgc
     1921 agttcttcct tccttcgaga ttacccgcga cgacgtggtc gacggggagg aatattttgt
     1981 ctccgtattt atcgagcgct tccttcgccc accccacgaa ctcgtcctcc actatggagc
     2041 tccctatgtt cacgcccttc gctttgagga atgtataagc ggcccctcct cccaggagca
     2101 ccctgtccgc cttttcaatg agactcttca aagcgtgtat cttgtccttg atcttgctcc
     2161 cgccgacgat taccaggaac ggccggtccg ggttctcacg gagccgcgtc aggaatttta
     2221 gctccctgct gacgaggaac cccgcgaccc tgaggtcgaa catggacgcc atgccgtatg
     2281 tggaggcgtg ctttctgtgc gatgtgccga acgcgtcgtt tacgtatata tcggcgtacg
     2341 aggcgagctc cttcgaaaac gcggggtcgt tgtcggtctc ttccttgtgg aacctgaggt
     2401 tctcgagcag catgacctcg ccgggctcga gcgcatccag cgcatccctg acctcgggtc
     2461 ctatgacttt tccgttgaac ttaactgact ttccgagcag atccgaaagc ctcatggcta
     2521 tgggcttcaa cgacaggtcc gcgatcgcta taccttttgg tctgccgagg tgcgatccga
     2581 gtatgacttt cgcctttctt tcgacgagat aatctatagt cggtaaagct cttcttatcc
     2641 tgtaatcctc gctgatcttt ccgtccctta tcggaacatt gaaatccact ctcacgaaca
     2701 ctcgcttgcc gtggaatact gaatcgggaa ggtcggatat cacgagttta tccatcacat
     2761 tttctcccct atgaactcga gtagatcgac caccctcgac gaatatccgt attcgttgtc
     2821 ataccacccg atcaccttca ccaggttgcc gtcgacgacg gacgtcagcg ccgaatcgaa
     2881 tacggcggaa tgcgggtctc cgatgaaatc cgatgatacg agctcctcgt ccgagtacct
     2941 aaggtagcct ttgagctccc ctgaggccgc ctgcctgaat ttttcgttca cctcttcagg
     3001 gctcgtgtgt tttttgactt cgcaagtgaa atcgacaact gacacgtcag cggtcgggac
     3061 cctcattgcg acagcgctc
//
LOCUS       DASSJH010000182         3837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_904277, whole genome shotgun sequence.
ACCESSION   DASSJH010000182 DASSJH010000000
VERSION     DASSJH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904277"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFJ67_03885"
     CDS             <1..469
                     /locus_tag="VFJ67_03885"
                     /inference="COORDINATES: protein motif:HMM:NF023786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7289556.1"
                     /translation="RIDSFREFPEALEGLDDKKQSKIVTYCTGGIRCEKATAYMISRG
                     FTDVYQLKDGIINFCQKYPDTVWEGKCFVFDDRLVSDVEAEGEVISRCVICGESSDRY
                     QNCRYPECDDLVILCKRCSEENEGCCSVECLEEYHAHNRIRTRERQGYKSRTA"
     gene            complement(753..>3837)
                     /locus_tag="VFJ67_03890"
     CDS             complement(753..>3837)
                     /locus_tag="VFJ67_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET7289557.1"
                     /translation="VGSADGVKRAMDRAVAGGEREQREGASGKWVAHWKMVHIIRPVW
                     ETAGQDNQMGKKFPPLTYTDEDADGLTLLEPAPRTIRGARNLISVALQYGNAFGQGFQ
                     AAALKPADFFGNDDILYLMEDMATGEIRLSILWEWVDKGAVLTEDDKETGVKAGDIFT
                     SDLFERLLAEEYDKLLKAKDKDVHDDSKPTTLPIAREIVETYVLDNIKTPWYIDLLNI
                     NLNNHDLSTAKIRIKQYMDAFKKDGTRITENLDFLPETRLDTTLEDELDSFEREVREM
                     KEWFAQPRFKGIRRLYSARQVVQQAGTIENDYTVARRYSEEFYSRLRELFAKGEQITT
                     FGPYSPGQIVTMKRIGIEGIYLGGWATSAKGSLSEDPGPDLASYPLSQVPDEAAGLVR
                     ALLTADRNQKFMRSRMSEKERKATPKVDYRPFIIADADTGHGGDAHVRNLIKRFVEAG
                     VTGYHIEDQKPGTKKCGHQGGKVLVPVDEQIKRLNAARFQLDIMKVPGIIVARTDAEA
                     ANLLDGRGDERDQPFILGATNTTVPGYKNCYLAILKRFYDKGITDVNGFMMYKISDAE
                     YDEVYAWFDKVGLTPIIDKSIQALKEGKERSITKPLENAASKFVETWEVESGLMTYAQ
                     AVAEQMEFAIEEGRNLGMTIEEWFEFANHSSYQHARDKARQMGIEAPWDCELSRTPEG
                     YYQVKGGIEFAIAKSLAVAPYADLIWMETKTANYEEAKEFAEAIHAEFPDKMLAYNLS
                     PSFNWDTTGMSEDEMRNFPKEIGKMGYVFDFITYGGHQIDGLAAEEFATALKQDGMLA
                     LARLQRKFRLLDSPYRTPQTLVGGPRLDGALAASSGRTATTVAMGKGSTQVQHLVETE
                     VPPRILEDWFELWSEHYGIPGRLSVKLRPHTAGSELLELTVKDEGGNKVADVIFATIH
                     DRWGNNFVSIRDQNTFEDKFRKKRLMALIHIFLIHRYKAGSVHYVSPTEDNQKQAQGM
                     KAMGIYDEVNTEIGHIIVARVNTERVKELLSTDLAELKNFIAKKSKPKGKKKDKQ"
BASE COUNT          686 a         1151 c         1052 g          948 t
ORIGIN      
        1 gaggatagat tcgttccgcg agttccccga agccctcgaa gggctcgatg acaaaaagca
       61 gagcaagatc gtgacctact gcacgggcgg gataaggtgc gagaaggcga ccgcctacat
      121 gataagcagg ggcttcaccg atgtttacca gctcaaggac ggaattatca acttctgcca
      181 gaagtacccg gacaccgtat gggaggggaa gtgtttcgtc ttcgacgaca ggctcgtctc
      241 ggacgtcgag gccgagggtg aagtcatctc acggtgcgtc atctgcgggg aaagctccga
      301 ccgttaccaa aactgtaggt accccgaatg tgacgacctc gtgatactgt gcaagagatg
      361 cagcgaagag aacgaaggct gctgctccgt tgaatgcctc gaagaatacc atgcacacaa
      421 ccgaataagg acccgcgaga gacaggggta caaatcaaga acggcctgac ggcgcagaac
      481 ctgtgcccgt agacgattct catgcggcta ctccgccgaa ccttcgctgg tttaagagaa
      541 taagctggaa agaaccgtaa aacacactac gtgtgtgctt agtcctttat gttcttcgcc
      601 ttcagcaaaa gggcgaggac gaacccgaga agaaagatcg cgtatagaaa aggcggaagc
      661 agctccatca tcgtttaaaa ctccctccgt cacggattga aaaacccgga ggcccttttt
      721 accggggcct ccgtaactcg ttttttaaaa gattactgct tgtctttttt cttgcccttg
      781 ggcttcgatt tcttcgcgat gaagttcttg agctcggcga ggtcggtcga gagcagctct
      841 ttaaccctct cggtattgac ccgggcgact atgatgtgcc cgatctccgt gttgacctcg
      901 tcgtatatcc ccatcgcttt catgccctga gcctgtttct ggttgtcctc ggtcgggctc
      961 acgtagtgta cggaaccggc cttgtacctg tgtatgagga atatgtgtat gagggccatg
     1021 agcctcttct tccggaactt gtcttcgaac gtgttctggt ctctgatgga aacgaagttg
     1081 ttcccccagc ggtcgtgaat ggtcgcgaat atcacgtcgg cgactttatt cccgccctcg
     1141 tccttgaccg tgagctcgag gagctcggag cccgcggtat gcggtctcag cttgacgctc
     1201 aggcggcccg gtatgccgta gtgctcggac cagagctcga accagtcttc caatatcctc
     1261 ggcgggactt ccgtctcgac caggtgctgc acctgtgtcg agcccttgcc catggcgacg
     1321 gtagtagccg tacgccccga cgatgcggcg agcgcgccgt cgagtctagg ccctccgacg
     1381 agtgtctgag gcgtcctgta aggcgaatcc agcagtctga acttcctctg gagcctcgcg
     1441 agcgcgagca tcccgtcttg cttgagagcc gtcgcgaact cctcggccgc gagcccgtct
     1501 atctggtgcc cgccgtaggt gatgaagtcg aacacatagc ccatcttacc gatctctttc
     1561 gggaagttcc tcatctcgtc ctcgctcatc cccgtcgtgt cccagttgaa tgaaggcgag
     1621 aggttgtagg cgagcatctt gtccgggaac tctgcgtgta tcgcttcggc gaattccttc
     1681 gcttcttcgt agttagcggt cttggtttcc atccagataa ggtcggcgta gggagcgaca
     1741 gcaagcgact tcgctatggc gaactcgatt ccgcctttaa cctgatagta accctcgggc
     1801 gtcctcgaaa gctcgcagtc ccacggggct tcgataccca tctgccgcgc cttgtccctc
     1861 gcgtgctggt atgaagaatg gttcgcgaat tcgaaccact cctcgatcgt catcccgagg
     1921 ttcctgccct cttctatcgc gaactccatc tgctcggcaa cggcctgggc gtaggtcatg
     1981 agtcccgact ccacttccca tgtctcgacg aacttactgg ccgcgttctc aagcggtttc
     2041 gttatgctcc tttcttttcc ttccttgagc gcctgtatgc tcttgtctat gataggcgtg
     2101 aggcccacct tgtcgaacca ggcgtaaacc tcgtcgtact cggcatccga tatcttgtac
     2161 atcatgaatc cgttcacgtc ggtgatgcct ttgtcgtaga acctcttcaa tatagcgagg
     2221 tagcagttct tgtatccggg caccgtcgtg ttcgtcgccc cgaggatgaa cggctggtcg
     2281 cgctcgtctc cccttccgtc gaggaggttt gcggcctcgg cgtccgtacg ggcgactatt
     2341 atccccggca ctttcattat atcgagctgg aaccttgcgg cgtttagcct ctttatctgc
     2401 tcgtccaccg gaaccagcac cttaccgccc tggtgtccgc atttcttcgt gcccggcttc
     2461 tggtcttcta tgtggtatcc ggtaacgccc gcttcgacga acctctttat gaggttcctc
     2521 acgtgtgcgt ctccaccgtg ccccgtgtcg gcgtctgcga ttatgaacgg cctgtaatcg
     2581 accttcggag tggccttcct ctccttctcc gacatgcgcg acctcatgaa cttctggttg
     2641 cggtcggcgg tgaggagagc gcgcaccagc ccggccgcct catcgggcac ctggctcagt
     2701 ggatagctcg ccaggtccgg gcccgggtct tcggacagcg accctttggc ggacgtagcc
     2761 cagccaccga gatagatacc ttcgatccct atgcgtttca tcgtgactat ctgtccgggc
     2821 gagtacggac cgaatgtggt tatctgctcg cctttcgcga agagctcccg gagcctgctg
     2881 tagaattcct cggagtatct cctcgctacg gtgtagtcgt tctctatcgt ccccgcctgc
     2941 tgcactacct ggcgggccga gtagagacgc cttatcccct tgaacctcgg ctgcgcgaac
     3001 cattccttca tctctctcac ttccctctcg aacgaatcta gctcgtcctc gagagtcgtg
     3061 tcgagcctcg tttccgggag gaagtcgagg ttctcggtga tcctcgtgcc gtcctttttg
     3121 aaggcgtcca tgtactgctt tatcctgatc ttcgccgtgg ataggtcgtg gttgttgagg
     3181 tttatgttga ggaggtcgat gtaccagggc gtcttgatat tgtcgagcac gtaggtctcc
     3241 actatctccc tcgcaatcgg gagcgttgtg ggcttggagt cgtcgtgtac gtccttgtcc
     3301 ttcgccttaa gcagcttgtc gtactcttcg gcgaggagcc tttcaaataa atccgaagta
     3361 aatatgtctc ccgcctttac gcccgtctcc ttgtcgtcct ccgtgagcac cgcacccttg
     3421 tccacccatt cccagaggat gctcaggcgt atctcgcccg tggccatgtc ctccattaaa
     3481 tatagtatgt cgtcgttgcc gaagaagtcg gccggcttga gcgccgccgc ctggaagccc
     3541 tgtccgaagg cgttaccgta ttgaagggcg acggagatga ggttcctcgc gcctctaatg
     3601 gtgcgtggcg cgggctcgag aagcgtgaga ccgtccgcgt cctcgtcagt gtacgtgagg
     3661 ggcgggaatt tctttcccat ctggttgtcc tgtccggctg tttcccagac gggccttatg
     3721 atgtgcacca tcttccagtg ggcgacccac ttgccgctcg cgccctcgcg ctgttcgcgc
     3781 tcgccgccgg cgaccgcacg atccatcgcg cgcttcacgc cgtcggcgga tcccacc
//
LOCUS       DASSJH010000183         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1555709, whole genome shotgun sequence.
ACCESSION   DASSJH010000183 DASSJH010000000
VERSION     DASSJH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555709"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFJ67_03895"
     CDS             complement(<1..513)
                     /locus_tag="VFJ67_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7289558.1"
                     /translation="MSSNGGNRRALRFVLLLGAVSLFGDMTYEAARSVIGPYMALLGA
                     SAAAVGTVAGLGEFIGYGFRIISGVFSDRTRRYWTITITGYVISLAAVPALALAGNWP
                     LAAALMMAERFGKAVRNPARDAMLSHAAHGMGRGWAFGLHEAMDQTGAIIGPAIVAGV
                     LVWKGSYTYGF"
     gene            complement(570..872)
                     /locus_tag="VFJ67_03900"
     CDS             complement(570..872)
                     /locus_tag="VFJ67_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289559.1"
                     /translation="MAELTEREKEFELFGMGIDDLEQLVRTRAAQQNIPVTHILIDWL
                     ELAQYQLEDRYRNVHHIINRLIYVLQSGMVIDNPAEAGKSETPDAVEKKPSKKKKD"
     gene            1003..1416
                     /locus_tag="VFJ67_03905"
     CDS             1003..1416
                     /locus_tag="VFJ67_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289560.1"
                     /translation="MMRKFVIAALLILTIPAGPARGEEYLPDSMKTHPEWYIIVSEWS
                     VYASWGGVAIIQGLTIENTSDLAYRNVKVRVTYTSNTAGGSGSVVNQESGVLPITLPP
                     HSKDTYLKAGYPFGAGSQFMNPLAIQVLGATPVSD"
     gene            complement(2025..2759)
                     /locus_tag="VFJ67_03910"
     CDS             complement(2025..2759)
                     /locus_tag="VFJ67_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289561.1"
                     /translation="MKSRLSYLKLYNALAVNVFLLWALVLSACAGAGTARTNVAPGGV
                     DSESRPEVINDEVLEMEKKYYVAGVLDTYNSLAGDAVRQREYRDEVINGRIYVIDYYY
                     NKFRQNMRSTKPDTNIVTDIAVLGLDAADAINPARRAKEILLAISGGLTGPDSSVDAD
                     DFYDEALPALISKMDSLRLERLVLIRQRMKYGTQGENPYTLGAAMIDVNNYFQSGTIS
                     GALLGITKSSGVAANEAEKELGGMSR"
     gene            2919..>3222
                     /locus_tag="VFJ67_03915"
     CDS             2919..>3222
                     /locus_tag="VFJ67_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289562.1"
                     /translation="MDINQIMNMRLIPGCIILVILALTPLRAQPVYSAESVPPAGQIP
                     SVIHVGVIPLNPFVITTNGKPKGISIELWEEIARSHGWKYEYTFLPATGYLDFVPEL"
BASE COUNT          645 a          957 c          893 g          727 t
ORIGIN      
        1 gaagccgtac gtgtagctcc ccttccagac gagtacgccc gcgactattg ccgggccgat
       61 tatcgcgccc gtctggtcca tcgcttcgtg gagtccgaac gcccagcccc ggcccatgcc
      121 gtgagcggcg tgggagagca tggcgtcgcg ggccgggttc ctgacggcct taccgaaacg
      181 ctcggccatc atgagcgcgg ccgccagggg ccagtttccc gcaagcgcca gggcgggtac
      241 ggcggcgagg cttatcacgt aaccggttat ggttatagtc cagtatctgc gggtgcggtc
      301 gctgaatacc ccggatatga tcctgaagcc gtatccgatg aattcgccca gccccgcgac
      361 cgttccgacc gcggcggcgc tcgccccgag gagtgccatg tagggcccga tgacgctcct
      421 cgccgcctcg tacgtcatgt cgccgaagag gctcaccgcc ccgaggagca gcacgaacct
      481 aagtgcccgc ctattcccgc cgttcgagct cattgggacc cgtcccgcat ggattaatga
      541 tacagaatta taagggattc aaagaggggt cagtccttct tctttttaga gggctttttc
      601 tcaacggcgt ccggggtctc gcttttcccg gcctccgccg ggttgtcgat gaccatgccg
      661 ctctggagca cgtatatgag gcggttgatt atgtggtgga cgttacggta cctgtcctcg
      721 agctgatatt gcgcgagctc gagccagtcg atcagaatat gcgtgacggg tatgttctgc
      781 tgcgcggccc tcgtcctgac gagctgctcc aggtcgtcga ttcccatgcc gaaaagctcg
      841 aattctttct ctctttcagt aagctccgcc atatcatatc tccttcgcgc gttcgtgtat
      901 cgtgcccccg gccccgctcc gggccgccgc ccggtttata ttacataagg attggattca
      961 taacacttgc cccgaaggag accccggcat atactatcaa tcatgatgag aaaattcgtt
     1021 atcgccgctc tattgatcct cacaatcccg gccgggcccg cccgcgggga ggagtacctg
     1081 cccgactcca tgaagactca cccggaatgg tacatcatcg tctccgagtg gtcggtgtac
     1141 gcatcgtggg gaggcgtcgc cataatccag ggcctgacca tagagaacac ctccgacctc
     1201 gcgtacagga acgtgaaggt aagggtcacg tacacctcga acacggccgg aggctcgggg
     1261 agcgttgtaa accaggaatc gggcgtcctc ccgattacgc tcccgccgca tagcaaggac
     1321 acctatctga aggcgggata cccgttcggc gcgggctcac agtttatgaa tccgctcgcg
     1381 atacaggtgc tcggcgcaac tcccgtttcc gactgaagca ggggcgtccg tactgtccca
     1441 atcaggctaa taatccgcct ttattgactt atatgaagtc ggagacatgc ttcgcctgat
     1501 acaccccagg tcagccggaa tcccgatatc cgcgagcgcg ggccccgcgc tggcggcggc
     1561 atgggttcga taacgggcca gctccaatgc taatacgcca ttatccgtcc cgcgaagcct
     1621 tgcgatttcc cgttacacac ggaattttcg acttttataa aaaaacatgc acgaaatgtg
     1681 gtgcaaaaaa attcaaactt tgctatactc ttattaaccg gttgttacaa cctgagcccc
     1741 gtcaactgaa gactagggac ctcttggcag ggaaccgaaa tcttccgggg ctctttagga
     1801 ctaattcctt attactaagt agtgcgggtt ggggtttttg ttttacaatc aaaaggctgg
     1861 agtgtagatt gaggcagggt taatttgtcc cggcgtagca ctatttaatg cgggtgagcg
     1921 gattattgcg tcgacacaac cggaaggcgg catggtaatc cgcgtgagcc cgtgacaccg
     1981 ggcttgcggc agattttttt ccttcaataa gcagcgggag cggtttaacg ggacattccg
     2041 ccgagctcct tctccgcttc gttcgcggct acccccgaag atttggttat accgagaagc
     2101 gcgcccgata tggtgcccga ttgaaagtag ttgttgacgt cgatcatggc ggccccgagc
     2161 gtgtaggggt tctccccctg agtcccgtat ttcatcctct gtcttattag cacgagcctc
     2221 tcgagcctga gagagtccat cttcgagata agcgccggga gagcctcgtc gtagaaatcg
     2281 tccgcgtcaa ctgacgaatc ggggccggtg aggccgcccg atatggcgag cagtatctcc
     2341 ttcgccctcc gggcggggtt tatcgcgtcg gcggcgtcga gccccagaac ggctatgtcc
     2401 gtaacgatgt tcgtatcggg ctttgtgctg cgcatgttct gcctgaattt gttgtaatag
     2461 tagtcgatga cgtaaatgcg cccgtttatg acctcgtccc ggtactccct ctgacgcacg
     2521 gcatccccgg cgagagaatt gtacgtatcg aggacgccgg cgacgtagta tttcttttcc
     2581 atctccagca cctcgtcgtt tatgacctcg gggcgggact cgctgtcaac tccgcccggg
     2641 gcgacgttcg tcctcgcggt ccccgctccc gcgcacgcgg agagcacgag cgcccacagc
     2701 agaaacacat tgactgctaa ggcgttgtaa agctttaaat acgataaacg gcttttcatg
     2761 tgtgtgatgc tcctctcaac ggcaatatta taccgtcttc cgatacccgc ttcagcagga
     2821 atttccagcc gtgcagcggg gcggcgctaa ctccccgccc cgccgccgtg gggatcgccc
     2881 gcggacatcg acattattga caccggaggc aaacggctat ggatattaat caaattatga
     2941 acatgcgcct catccccgga tgcattatcc tcgtaatcct ggctcttacg ccgctccggg
     3001 cacagcctgt gtactccgct gagagcgttc cccccgccgg gcaaattccg tctgtcatcc
     3061 acgtgggggt gattcccctc aatcccttcg tcatcacaac taacggcaaa ccgaagggga
     3121 tatccataga gctttgggag gagatagcca ggagccacgg atggaagtat gaatatacgt
     3181 tcctgccagc aactggttat ctggatttcg tacccgagct cg
//
LOCUS       DASSJH010000184         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1341579, whole genome shotgun sequence.
ACCESSION   DASSJH010000184 DASSJH010000000
VERSION     DASSJH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341579"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /gene="xseA"
                     /locus_tag="VFJ67_03920"
     CDS             <1..837
                     /gene="xseA"
                     /locus_tag="VFJ67_03920"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7289563.1"
                     /translation="EPKGVGAQALALEQLKEKLLAEGLFSPERKRPLPFLPRRIGIVT
                     SPTGAAIKDILKVLDSRFPNLEIIVSPARVQGIEAPGDIITSLNRLYVLPGIDLIILA
                     RGGGSKDDLGAFNDEALARVIAKSPVPLVSAVGHEIDFTIADLVADVRAATPSNAAEI
                     VVREKAELLQGIAELKAKIAFALLNRVEFHAREIDQMQSDMARLFQIKMDNAAAETGA
                     LAGKLDALSPLKVLDRGYSITYKLPSMKNVRDSKELKRGDKVLIAFAKGKARCSVTES
                     ED"
     gene            complement(834..1847)
                     /locus_tag="VFJ67_03925"
     CDS             complement(834..1847)
                     /locus_tag="VFJ67_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7289564.1"
                     /translation="MSEKTIPELLQNRVKEHGTKLLYQRRDGWSWKQITWLDFDRKVR
                     NIAAFLHDLGFGRGDRALVVSSNRLEAISSELAVFHLGGVIVPLSPGESAAKIIEAAN
                     ELHPKLIFIEQGAAAEGILGTLPGLGSVARVIIFPDARISNERVLNFKAVLKFGLMKR
                     KKLEDELAGIYKKITPDLPAAIFISPDGSSTLKAEVSEGDLIESLEKASRRCPDLNAE
                     DQAFSYITEASPFAKFVNYLTIYQGSRAAVAEGRKDFYQDIVEVMPTVLYATKAGLEE
                     LCGKSGASLNGRKPGKSLRMDLGNRVRHILTDSHPGGETETIIRSAGALLIEVPEFNL
                     LLG"
     gene            1929..3299
                     /locus_tag="VFJ67_03930"
     CDS             1929..3299
                     /locus_tag="VFJ67_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET7289565.1"
                     /translation="MKKVVKIKPIESELFDIARHLVTQLRSSGHKAFCVGGCTRDMMM
                     GVPPKEYDITTSATPDEVSKIFSHTIPVGVSFGVILVLIGHYRFEVATFRKEESYSDS
                     RHPDLVTYSTEEEEDVLRRDFTINGMLYDPIAGEAIDFVGGTDDIRDGIIRTIGVPYQ
                     RFDEDKLRLMRAVRFASRYGFEVESETYRALTELASGILLVSAERIRDELVKIITQKN
                     PGAGLDLLRVSDLLRHFLPEVDVMYGVEQPPEFHPEGDVFTHTCLVLDRIYENTEGLA
                     SPELAMAGLLHDVGKPPTFSVSDRIRFNGHDKLGADMSREICRRLKFSNRQIELIYEL
                     IRDHLKFKDVFHMKKSTLKRFIGQTDFEEHMILHLADCLASHGSTEAYDFVMKKFDEF
                     KDEEIKPEPLIGGRELIEMGYKPGPIFTEILNFVEEAQLEGEIQSTDEARELVSRKYP
                     IGKSAV"
BASE COUNT          804 a          898 c          915 g          689 t
ORIGIN      
        1 gagcctaagg gggtcggggc acaggcccta gccctcgaac agctgaagga gaagctcctg
       61 gcggaagggc tgttcagccc cgagaggaaa cgtcccctcc ccttcctgcc ccgaaggatc
      121 ggcatcgtga catcccctac cggggcagcg ataaaagaca ttctaaaagt tttagacagc
      181 aggttcccga acctggagat aatagtctcg ccggcgaggg tacagggaat agaggcgccg
      241 ggagatataa ttacatctct caacaggctc tacgtgcttc ccgggataga cttgatcata
      301 ctggcgagag gcggcggctc gaaggatgac ctgggcgcgt tcaacgacga ggcgcttgcg
      361 agggtgatag cgaaatcccc agtccctctg gtatcggccg tcggacacga gatcgatttt
      421 acgatcgccg accttgtggc ggacgtaagg gcggctacgc cctcgaacgc cgctgaaata
      481 gtcgtaaggg aaaaagcgga gctccttcag ggcattgcgg agctcaaggc caagatagcc
      541 ttcgccctct tgaaccgagt cgaattccat gcgagagaga tagaccagat gcagtccgat
      601 atggcaaggc tcttccagat aaagatggat aacgccgccg ccgaaacggg tgcactcgcc
      661 gggaagctcg acgcgctcag ccctctgaaa gttctcgacc gcggttacag catcacctac
      721 aaacttcctt ccatgaaaaa cgtcagggat tcgaaggagc taaaacgcgg agacaaggtc
      781 ttgatcgcct tcgccaaggg aaaggccagg tgctcggtca cggagagcga agactaaccc
      841 aggagaaggt taaactcggg cacctcgata aggagtgccc cggcgctcct tataatcgtt
      901 tcggtttccc ctccaggatg tgaatccgta agtatatgcc tcactctgtt gccgaggtcc
      961 atcctgagac tcttccccgg cttcctgccg tttaggctcg cgccggattt accgcacagc
     1021 tcctcgaggc cggctttcgt cgcatacaag acagtcggca ttacctccac tatgtcctga
     1081 taaaaatcct ttctgccctc ggcaacagcg gccctgctgc cctgatagat agtgagataa
     1141 ttgacgaact tcgcgaacgg gctcgcctcc gttatatatg aaaacgcctg gtcttcggcg
     1201 ttgaggtcag gacacctgcg ggaggctttt tcaagggatt caatcagatc cccttccgaa
     1261 acctcagcct taagcgtact gcttccgtcg ggagatatga atatcgcggc aggaagatcg
     1321 ggcgtaattt tcttatatat accggcgagc tcgtcctcga gcttcttcct tttcattaag
     1381 ccgaacttga gcacagcctt gaagttgagg accctttcgt ttgaaatcct ggcatcgggg
     1441 aatattatca ctctcgcgac gcttcccagg cctgggagcg tgcccaatat accctcggcg
     1501 gcagcaccct gctcgatgaa tatgagcttc ggatgaagct cgttcgcggc ctcgataatc
     1561 ttcgcggcgc tctcccccgg agacaacgga acgatcactc ccccgaggtg aaagacggca
     1621 agctccgaag agatcgcctc gagcctgttg gaagagacca ctagcgccct gtcccccctt
     1681 ccgaacccga ggtcgtgcag gaatgccgcg atattcctga ctttacggtc gaagtcgagc
     1741 catgtaattt gtttccagct ccagccgtcc cgcctctggt aaaggagctt ggttccgtgc
     1801 tcttttactc gattttgcag aagttctggt atagtctttt cgctcataaa ctgttaccgc
     1861 gagtgatgcg tctaaatccg ctcccggata gagtgtaaaa tatactataa aactgccggt
     1921 ttaaaactat gaagaaagtc gtaaaaatca aaccgatcga gagcgagctc ttcgacatag
     1981 cgagacatct tgtcacccag ctaaggagct cgggacacaa ggctttctgc gtcggcggtt
     2041 gcacgaggga catgatgatg ggcgtcccgc ccaaggaata tgacataacg acgagcgcca
     2101 cgcccgacga agtatcgaag atattctcac atacaatacc cgtcggagtg agcttcggcg
     2161 ttatactcgt ccttatcggc cattacaggt tcgaggtcgc cactttcagg aaagaggaga
     2221 gctactcgga cagccgccac ccggacttgg tcacatacag caccgaggaa gaagaagacg
     2281 ttctcaggcg tgatttcacc attaacggaa tgctctatga cccgatcgcc ggggaggcta
     2341 tagatttcgt gggcgggacg gacgatataa gggacgggat aatcaggacc atcggcgttc
     2401 cctaccagag gttcgacgaa gacaagctca ggctcatgag ggccgtgaga ttcgcatcga
     2461 ggtacgggtt cgaggtcgag agcgagacgt accgagctct cacggagctc gcgtccggga
     2521 tactgcttgt gagcgccgag aggattaggg acgagctcgt gaagatcatc acacagaaga
     2581 accccggagc cgggctcgac cttctccgtg taagcgatct cctccgtcat ttcctgcctg
     2641 aagtggacgt tatgtacggt gtggagcagc cgcccgaatt ccatcccgag ggtgacgtat
     2701 tcacacacac gtgtctcgtg ctcgacagaa tatacgagaa taccgaaggg ctcgcctcac
     2761 cggagctcgc catggcgggg ctcctccacg acgtaggcaa gcctccgacg ttctccgtct
     2821 cggatcgaat aagattcaac ggccacgaca agctcggggc tgatatgtcg cgcgagatat
     2881 gcaggcgact caagttctcg aacaggcaaa tcgagcttat ttatgagctg ataagggacc
     2941 atctcaaatt caaagacgtt ttccatatga aaaagagcac gctgaagaga tttatcgggc
     3001 agaccgattt cgaggagcac atgatcctgc atctcgcgga ctgcctggcg agtcacggct
     3061 cgaccgaggc ttacgacttc gtgatgaaaa agttcgacga gttcaaggac gaggagataa
     3121 aacccgagcc gctcatcgga gggagggagc ttatcgaaat gggttataaa ccggggccga
     3181 tattcacgga gatattgaat ttcgtcgagg aagcccagct cgaaggggaa attcagagta
     3241 cagatgaagc ccgcgagctc gtttcgagaa aatacccgat cggcaagagc gctgtttaga
     3301 tcgttc
//
LOCUS       DASSJH010000185         5381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1341909, whole genome shotgun sequence.
ACCESSION   DASSJH010000185 DASSJH010000000
VERSION     DASSJH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341909"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="VFJ67_03935"
     CDS             complement(<1..97)
                     /locus_tag="VFJ67_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET7289566.1"
                     /translation="MRLILFGPPGAGKGTQAKKITDKYMTAHISTG"
     gene            complement(113..1432)
                     /gene="secY"
                     /locus_tag="VFJ67_03940"
     CDS             complement(113..1432)
                     /gene="secY"
                     /locus_tag="VFJ67_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET7289567.1"
                     /translation="MNRNIGAIPRIPELNKRLLFTAVMLIVYRLGVFVPIPGIDPEQI
                     LKLFEQTKGTIFDILNMFSGGALQRASVFALGVMPYITSSIIMSLLVKAFPSLEAMQK
                     EGEAGRRKINQYSRYGTVLICLVQGFMLAITLEGGIGSGATVVKEAGWTFRIMTVLTL
                     TAGSLFVMWIGEQITERGIGNGISLIIAASIIVGLPGGLWNIIRLISTGEMTVFGVAL
                     ILILLVLIVGFIVFMERSYRKIPVQYPRRVVGRKVFGGQSSHLPLKTNTAGVIPPIFA
                     SSLLIFPATILTFINVPFLRNMSDLLFQNFIVYNIIFAGLIIFFAYFYTAIIYNPDDL
                     ADNLRKNGGFIPGIRPGKKTSEYIDRILGRITFIGAIYVAFICVIPSLLQREPFNLPF
                     HFGGTSLLIVVGVTLDFVQQIESFLITHSYEGFVRKKGMKGPRRYNV"
     gene            complement(1429..1860)
                     /gene="rplO"
                     /locus_tag="VFJ67_03945"
     CDS             complement(1429..1860)
                     /gene="rplO"
                     /locus_tag="VFJ67_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET7289568.1"
                     /translation="MLNNLSPQPGSKKNKKRVGRGTGSTGKTSGKGHKGQRARSGGRV
                     SAWFEGGQTPLKMRSPKRGFTSRKTLKFDVINVSDLNRFGEGEVVTREEFASAGLVRG
                     KNPVKVLGNGELERSLTVRADAFSKSAVAKIEDKGGKADTI"
     gene            complement(1949..2473)
                     /gene="rpsE"
                     /locus_tag="VFJ67_03950"
     CDS             complement(1949..2473)
                     /gene="rpsE"
                     /locus_tag="VFJ67_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET7289569.1"
                     /translation="MQKEKIDPSEFELKEKVVHIRRVAKVTKGGKRFHFTALAVVGNS
                     AGIIGSGLGKSNEVPDAIRKAIEKAKKGLIRVPIQGSTIPHEVFGEHVSSTVMLRPAT
                     PGTGVIAGGAVRAVVELAGIHDILSKVVGSTNPLNVVKAAINGLSMLRMPREVASVRG
                     KELTELDLPKSVLS"
     gene            complement(2513..2884)
                     /gene="rplR"
                     /locus_tag="VFJ67_03955"
     CDS             complement(2513..2884)
                     /gene="rplR"
                     /locus_tag="VFJ67_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET7289570.1"
                     /translation="MKRASRKSKRDVRHVRVRRKLTGTPDRPRLVVFKSSKHMYAQII
                     DDLKAHTLLSASSLTPKVKEMLGQEKSNKTDSAKIIGKYIGELSVSMGIKEVCFDRGG
                     YPYHGRIKAFADGAREAGLVF"
     gene            complement(2886..3434)
                     /gene="rplF"
                     /locus_tag="VFJ67_03960"
     CDS             complement(2886..3434)
                     /gene="rplF"
                     /locus_tag="VFJ67_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET7289571.1"
                     /translation="MSRIGRKPIKVPGGVKISLGKNTVQVEGAKGKLSWDIPEGVSAK
                     IEDENILIDRRNDEKKIKALHGLSRAVISNMVTGVSEGFTRTLEIVGVGYRAEALGKS
                     QIKFSLGYSNPVEFSLPEGVSVAVEERGTRLILQGIDKQVVGQTAARIRELRPPDSYK
                     GKGIRYSAEMMKLKPGKAGAKG"
     gene            complement(3445..3837)
                     /gene="rpsH"
                     /locus_tag="VFJ67_03965"
     CDS             complement(3445..3837)
                     /gene="rpsH"
                     /locus_tag="VFJ67_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET7289572.1"
                     /translation="MTDPIADMLTRIRNAIIAGHKSTLLPTSKMKTELARILKEQGYI
                     ADYKAEDQDGRKVINITLAYTPDRESVIKEIRRLSKPSRRVYVNKDEIPRIKGGLGVC
                     ILSTSKGIMTGAQARRQGIGGELICSIL"
     gene            complement(3885..4070)
                     /locus_tag="VFJ67_03970"
     CDS             complement(3885..4070)
                     /locus_tag="VFJ67_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET7289573.1"
                     /translation="MTRKAMMEKSIREPKFKVRSRNRCPLCGRPRAFLRKFNMCRLCF
                     RDLSSHGKVPGVRKSSW"
     gene            complement(4084..4638)
                     /gene="rplE"
                     /locus_tag="VFJ67_03975"
     CDS             complement(4084..4638)
                     /gene="rplE"
                     /locus_tag="VFJ67_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET7289574.1"
                     /translation="MVPRLKEVYKTKVVPSLKNKFSYKNDMEIPRIEKIVINMGIGRL
                     SDGGKDKKIIEEAAEELSLITGQKPMVRAAKKSIAGFKLREGVPVGCSVTLRGSKMYE
                     FLDRLINLTLPRVRDFRGVSAKAFDGSGNYTLGLKEQIIFPEIDYSKVQHVKGMNITI
                     VTTARTDEESRGLLKEFGMPFVAN"
     gene            complement(4638..4994)
                     /gene="rplX"
                     /locus_tag="VFJ67_03980"
     CDS             complement(4638..4994)
                     /gene="rplX"
                     /locus_tag="VFJ67_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET7289575.1"
                     /translation="MGAKAIKDTKNRRKLNIKSGDTVYVVAGKEKGKTGKVIKVLHKK
                     NGALVEKLNIVKRHQRQSQKNPTGGIVEKESPIHISNLMIYDPSERKPVRIGRKILSG
                     GEKVRYSKKSGEEIKK"
     gene            complement(4996..5364)
                     /gene="rplN"
                     /locus_tag="VFJ67_03985"
     CDS             complement(4996..5364)
                     /gene="rplN"
                     /locus_tag="VFJ67_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET7289576.1"
                     /translation="MISSTTFLESADNTGAKRLYCIKVLGGTRRKYARLGDIVVVSIK
                     EAIPNSKVEKGTVHRAVIVRTRKEQRRTDGSYVRFDNNAAVLITKDNEPLGTRVFGPI
                     ARELRTKGFMKIISLAPEVV"
BASE COUNT         1102 a         1584 c         1221 g         1474 t
ORIGIN      
        1 cgcctgtcga aatgtgagct gtcatgtact tatccgttat cttcttcgcc tgtgtcccct
       61 ttccggcacc gggcggacca aataaaatca atctcattca ataactccaa tctcaaacgt
      121 tgtatcgtct cggtcccttc atgcctttct tcctgacgaa cccctcataa ctgtgagtta
      181 tcaggaagga ctcgatctgc tgaacgaaat cgagagtcac tcccacaacg atcagaaggg
      241 atgtgccgcc gaaatggaac ggaagattaa aaggctccct ctgcagtaac gagggtatca
      301 cgcaaataaa agctacgtat atagcaccta taaaagtaat tcttcccagg attctgtcaa
      361 tgtattcgga cgttttcttg ccgggcctga tgccgggtat aaaccccccg ttctttctca
      421 gattatcggc cagatcgtcg gggttgtata tgattgccgt ataaaaatac gcgaaaaata
      481 ttatgagtcc agcgaatata atattataca caatgaaatt ctgaaataat aaatcagaca
      541 tattcctcag gaagggcacg tttatgaacg tgagtatcgt cgcggggaaa attagcagcg
      601 aggacgcaaa tataggaggt atgacaccgg ccgtattagt cttaagcggc aggtgcgaag
      661 actgacctcc gaataccttt ctcccgacga ccctcctagg gtactgtacc ggaatttttc
      721 tataagacct ctccatgaac actataaagc cgacgatgag gacgagaagg atcagtatga
      781 gcgccactcc gaacacggtc atttcgccag tgcttatgag ccttattatg ttccacaagc
      841 ctcccggcag gcccacgatg atcgatgccg cgataatgag agagatgccg ttcccgatgc
      901 cgcgctccgt aatctgctcg ccgatccaca tcacgaaaag agatccggct gtgagcgtga
      961 gcaccgtcat tatcctgaag gtccatcccg cctccttcac cacagtcgcg cccgagccta
     1021 ttccgccctc aagcgtgatc gccagcatga agccctgaac gaggcagatc agcaccgtgc
     1081 cgtaacgcga atactggttg atttttcttc ttccggcctc gccttccttc tgcatggcct
     1141 cgagcgaagg aaaagccttg accagaaggg acattatgat cgaagacgtg atatagggca
     1201 tgacgcctaa cgcgaaaacg gacgccctct gaagcgcgcc gcccgaaaac atattcaaga
     1261 tgtcaaagat cgtgcccttc gtctgctcaa agagctttaa aatctgctcg gggtctattc
     1321 ccggtatagg gacgaaaacg ccgagcctgt acactataag cataaccgcc gtgaaaagaa
     1381 gacgtttatt gagctcgggg attctgggaa tagcgcctat attcctattc atatagtatc
     1441 tgccttaccg cccttatcct cgatcttggc cacggcgctc ttgctgaagg catccgctct
     1501 cacggtgagc gagcgttcga gctcgccgtt ccccaacacc ttgacggggt tcttgccccg
     1561 cacgaggccc gcgctggcga attcttcacg tgttaccact tcgccctcac cgaacctgtt
     1621 caggtccgag acatttatta cgtcgaattt aagggtcttg cggctggtga agcccctctt
     1681 cggagacctc atcttgagag gcgtctgtcc gccttcgaac caagcggaga ctctgcctcc
     1741 cgacctcgcc ctctggcctt tgtgtccctt accgctcgtt tttcccgtgc tccccgtgcc
     1801 cctgccgact cttttcttat tttttttcga tccgggctga ggagatagat tattaagcat
     1861 tttcgatgac cctcttaaat aaagaatttt tcatccctgt ccgttcccga ccgtatcggg
     1921 gacaggggtc ggttacgcat tatcgatttc agctcagaac ggatttcgga agatccagtt
     1981 ccgtcagctc tttccctcgg acactcgcga cttccctcgg catcctcagc atggacaacc
     2041 cgtttatagc ggccttcact acgttcagag ggtttgtgga tcctacgact ttcgagagaa
     2101 tgtcgtgtat cccggcgagc tcgacgaccg ccctcactgc gcccccggca ataacacccg
     2161 tacccggcgt cgcgggtctc agcataaccg tgctcgacac gtgctcgccg aaaacctcat
     2221 ggggaatagt gctgccctgt atagggaccc ttataagccc cttcttcgct ttctctatag
     2281 ccttgcggat tgcatcgggc acttcgttag atttgccgag gccagagccg atgatcccgg
     2341 cggagttgcc tacaacggca agcgcggtga agtggaacct ttttccgccc ttggtgacct
     2401 tggcgacacg ccttatgtga acgacctttt cctttagctc aaactcactc gggtctatct
     2461 tttccttttg caagcatacc tccctcgaac gacaaattaa tcgtacttgt tgttaaaata
     2521 cgagccctgc ttccctcgcc ccatcggcga aggccttgat ccttccgtga taggggtatc
     2581 cgccgcggtc gaaacagacc tctttgatac ccattgagac ggaaagctct ccgatatact
     2641 taccgattat ctttgcggaa tcggtcttgt tcgatttctc ctgaccgagc atttccttaa
     2701 ctttcggcgt gagcgaagaa gcggagagaa gcgtgtgcgc cttcagatcg tcgattatct
     2761 gcgcgtacat gtgcttagac gacttgaaga cgacgagcct cggcctgtca ggagtgccgg
     2821 tcaatttcct tctgactctt acgtgcctca cgtccctttt tgattttctg ctagctcttt
     2881 tcattttatc cctttgcacc agcctttccg ggtttcaatt tcatcatttc agccgagtac
     2941 ctgataccct tgcccttgta cgaatcgggc ggcctcagct cccttatcct cgccgcggtt
     3001 tgtccgacga cctgcttgtc gatcccctgg aggattagcc tcgtacccct ttcctcgacg
     3061 gcaaccgaca cgccttcggg gagtgaaaac tcgaccgggt tcgaataccc gagactgaat
     3121 ttgatctggc tcttgccgag cgcctcggcc ctgtaaccga caccgacgat ctcgagggtg
     3181 cgcgtgaagc cctcgctcac gcccgtgacc atgttggata tgacggcgcg ggagagaccg
     3241 tgaagcgcct ttatcttctt ctcgtcgttt ctgcggtcga tcaggatatt ctcgtcctcg
     3301 atcttggcgc tgacaccttc ggggatgtcc cacgaaagct tgcccttggc gccttcgacc
     3361 tgcactgtgt tcttcccgag gcttatcttt actccgcccg gaacttttat cggttttctt
     3421 ccaattcgag acattcagac tacctcacaa tattgaacag ataagttcgc cgcctatacc
     3481 ctgcctgcgc gcctgcgcgc ccgtcataat ccccttggac gtggacagga tgcagacacc
     3541 aagcccgccc tttatgcgcg gtatctcgtc tttattgacg tacaccctgc ggctcggctt
     3601 gctgagcctg cggatttcct ttatcacgct ttccctgtcc ggggtgtacg cgagcgtaat
     3661 gtttataacc tttcttccgt cctggtcttc tgccttgtaa tccgctatgt aaccctgctc
     3721 cttcaaaatc ctcgccagct ccgttttcat tttggaggta ggcaagagcg tggacttgtg
     3781 tcccgctatt attgcgtttc taatccttgt taacatatca gctattgggt ctgtcatttg
     3841 tcaatcgccc ctttattgga atccgtcggc aatataattt tcatctacca gctcgatttc
     3901 cttacacccg gaaccttgcc gtggctggag agatccctga aacagagcct gcacatgttg
     3961 aacttcctca agaaggccct cggtctgccg cagagaggac agcggttcct cgaccttact
     4021 ttgaacttcg gctccctgat cgatttttcc atcattgctt ttcgtgtcat taagccttct
     4081 ccattaattc gcaacaaacg gcataccgaa ctcctttagg agccccctcg actcttcgtc
     4141 ggtccgcgcc gtcgtcacaa tcgtgatatt cattcctttt acatgctgta ccttgctgta
     4201 atcgatctct gggaagatga tctgctcttt aagacccagc gtgtaattcc cgctcccgtc
     4261 gaaagccttc gccgagaccc cgcggaagtc cctcaccctg gggagcgtga ggttgatgag
     4321 cctgtccagg aactcgtaca tcttactgcc cctcaaagtg accgaacagc cgacgggcac
     4381 gccctcgcgt aatttgaacc ccgcgatgga tttcttggcc gctcttacca tcggtttctg
     4441 accggtgatg agcgagagct cctccgcggc ctcttctatt atcttcttgt ccttccctcc
     4501 gtcgctcagc ctgccgatac ccatgttgat gacgatcttc tcgatcctgg gtatctccat
     4561 gtcgttctta tacgagaact tgttctttaa agagggaacg actttggtct tgtaaacttc
     4621 tttaagcctg ggaaccatta tttcttgatc tcctctccac tcttcttgct gtatctcact
     4681 ttctcgccgc ccgacagtat cttgcgtccg atgcggacag gttttctctc ggacgggtcg
     4741 tagatcatca ggttggatat gtgtattggc gattccttct ccactatacc gcccgtcggg
     4801 tttttctggg actggcgctg atgcctcttc actatattga gcttctcgac gagtgcgccg
     4861 tttttcttat ggagcacctt tatgaccttc ccggtcttac ccttttcctt gcctgcaact
     4921 acgtagacgg tgtctcccga cttgatattc agcttccttc tgttttttgt atccttaatc
     4981 gcctttgcac ccactttaga ctacctccgg cgcaagagaa ataattttca tgaatccttt
     5041 cgtacgcagc tccctggcta ttggaccgaa cacgcgggtc cccaaaggct cattgtcctt
     5101 cgtaataaga actgcggcgt tattgtcgaa cctgacgtat gagccgtccg tccttctctg
     5161 ctcttttctc gtcctcacta taaccgctct gtgcactgtg cccttctcaa ccttggaatt
     5221 aggtatggct tctttgatcg ataccaccac tatgtcgccg agcctcgcgt attttctgcg
     5281 agtgccccca agcactttta tgcagtagag cctcttggcc ccggtattgt cggccgattc
     5341 caaaaacgta gtcgagctaa tcactcggag gtccctcccg t
//
LOCUS       DASSJH010000186         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_581614, whole genome shotgun sequence.
ACCESSION   DASSJH010000186 DASSJH010000000
VERSION     DASSJH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581614"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFJ67_03990"
     CDS             complement(<1..521)
                     /locus_tag="VFJ67_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET7289577.1"
                     /translation="MYYKDYDTEGFFDEYFLPDGSPRDGASILLKRLEQLPGGELLRR
                     QKATEARLFQMGVTFTVYGEEEGTERIFPFDIIPRVIEAREWESLEKGLKQRIYALNL
                     FIDDVYNSKKIIKDGVIPEELLQTSKCYRPQCEGFKPPRGIWCHISGTDLVRGGDGVY
                     YVLEDNLRCPSGVS"
     gene            complement(818..1210)
                     /locus_tag="VFJ67_03995"
     CDS             complement(818..1210)
                     /locus_tag="VFJ67_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289578.1"
                     /translation="MDNDSVLNSIKAAFNLGVWMRQNQPGKSASDAGRSLGETIRLNL
                     RQPEEGQYEKDVIEANVAYLTDIALLGYLMSGVCSYDHEIKEKIPYLIEKRLISESYI
                     DAPSDYEGKVADSEDGNYFTRPKAVGMN"
     gene            complement(1770..>2404)
                     /locus_tag="VFJ67_04000"
     CDS             complement(1770..>2404)
                     /locus_tag="VFJ67_04000"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET7289579.1"
                     /translation="YIFIDNGGKVSQGFLETYEGTGDNPVEQDILATLDNLVMTGDYD
                     TLYITGHSLGGALAVLAFPDLSQKVSIENVLMYNFAGPAAGDSDFVSAYEDEYGTNRV
                     SWRIVNANDLVPKLPPLGLDCPDFSYFHVSGEFQITFGVGLPALPDFSAADCDLITIG
                     AQVVTYGLNNQDGIIEDHKLCTYFTTLCNQGSDPSTCAERAIGCGTAESP"
BASE COUNT          505 a          683 c          603 g          613 t
ORIGIN      
        1 gagacgcccg acgggcatct cagattgtcc tcgagcacgt agtaaacgcc gtcccctccc
       61 ctcacgaggt ctgtccctga aatatggcac cagatgcctc tcggcggttt gaacccctcg
      121 cactggggcc tgtagcactt gctcgtctgc aggagctctt cgggaatgac gccgtccttg
      181 attatctttt tgctgttgta aacgtcgtct atgaaaaggt tgagcgcgta tatcctctgc
      241 ttgagcccct tctccagaga ctcccattca cgcgcctcta tgaccctcgg gattatgtcg
      301 aacgggaata tcctctcggt cccctcctcc tcgccgtaga cggtgaacgt cacccccatc
      361 tggaagagcc tcgcctcggt ggccttctgc cgcctaagca gctccccgcc ggggagttgt
      421 tctagcctct tcaggaggat cgatgcgccg tccctggggc ttccgtccgg aaggaagtac
      481 tcgtcgaaaa acccctctgt gtcataatcc ttgtaataca taaccattcc ttcagtttag
      541 catttttttc tattcctcac attatccgtt cccgtccgct ctcccgctcc gccccagcgc
      601 acggcatttt ttatttcccc gggccaagag agacaggggg gatggcccgg ggataggcta
      661 aaggtgtgtc agttgattag taatccggat accgcacgcg gctacggtcg aacgtcgctt
      721 aaccggccgg gtaaaacacc cggagacggg aacacccgca tattcccgtc tccggctaca
      781 taaggaggta tgaagaaatg agattacatt cgtcatgtca gttcattccg accgctttcg
      841 gcctggtgaa ataattcccg tcctccgagt cggcgacctt cccttcgtaa tccgagggcg
      901 cgtcgatata gctctcgctt atgagcctct tctctatgag gtaagggatc ttttccttta
      961 tttcgtggtc gtaagagcat acacccgaca tcaggtagcc caagagagcg atgtccgtga
     1021 ggtaggccac gttcgcctct atcacgtcct tctcgtactg gccttcctcc ggctgacgaa
     1081 ggttgagcct tatagtttcc ccgagagaac gcccggcgtc tgaagcgctc tttcccggct
     1141 ggttctgcct catccaaaca cctaaattaa atgccgcctt gatcgagttt aacacgctgt
     1201 cgttatccat cgtccttcct cctccataga gtttgagatg ctccgacccg tttccccctt
     1261 taaacccgtt aacgcttcct taatttgtac gcgtttgcgt atgttttata cccaaagctt
     1321 tcggtaatat atgtggcaat aatcgtgcca atgccgtaaa caaaggggaa ttataaggaa
     1381 tctaatgagt ttactagacc ttacatttat tagcagttgc ggtgaaagtg tgcctaaaat
     1441 ataaaatttg cctgaaaact gaacatgaag gtattaattt ataagcattt cagggggaaa
     1501 ttaagcagcg tacttaagat attactttac ctccgccgca attagttatg tcacttcggg
     1561 cgaggcaaga caatcccgtt tttcaatttt aataatcgca ccaggatggt gcccctatag
     1621 gatcggtcag ccgcctcgca ggagcgagtt cctgaatcga gttcaggaca ggccagccgc
     1681 gattcgactt acttgtcgct ccaggatggc gctcctatgg tgatgtttcc atattttatc
     1741 ctgcatcatg cagcttgcac cttctttcct cacgggctct cggcggtgcc gcatccgatc
     1801 gctctctcgg cgcacgtcga ggggtcggac ccctgattgc atagcgtcgt gaaatacgtg
     1861 cacaatttgt ggtcctcgat tatgccgtcc tgattattga gaccgtatgt cacgacctgc
     1921 gcgcctatgg tgatcagatc gcagtcggcg gcgctgaagt ccggaagcgc cggaagcccg
     1981 acgccgaacg ttatctggaa ttccccgctc acgtggaagt acgagaaatc ggggcagtcg
     2041 agaccgagcg gtggaagctt agggacgagg tcgttcgcgt tgacgatccg ccagctcacg
     2101 cggttagtgc cgtattcatc ctcgtacgcg ctcacgaaat cgctgtcccc ggcagcaggc
     2161 ccggcgaagt tgtacattaa gacgttctct atcgaaacct tctgcgagag atcggggaaa
     2221 gcgagcaccg cgagcgctcc gccgaggctg tgccccgtaa tgtacagcgt gtcgtagtct
     2281 cccgtcatga cgaggttgtc gagcgtagcg agaatgtcct gctcgacggg attatcgccc
     2341 gtgccctcgt acgtttcgag aaacccttga gataccttgc cgccgttgtc gataaagata
     2401 tagt
//
LOCUS       DASSJH010000187         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1342835, whole genome shotgun sequence.
ACCESSION   DASSJH010000187 DASSJH010000000
VERSION     DASSJH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342835"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..729)
                     /locus_tag="VFJ67_04005"
     CDS             complement(76..729)
                     /locus_tag="VFJ67_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7289580.1"
                     /translation="MPKLTIDGIEVEVEDGLNLIQAASRVGIDVPHFCYHPALSIVAQ
                     CRQCLVEVEGVPKVMPACNTFVREGLIVKTNTEKAIKARKATVEFTLINHPLDCPICD
                     KGGECPLQLTTVKHGPGYSRFEGPEEKKVRRKYYLSDRILYDPNRCIMCTRCVRFTDE
                     VTKTGELGFDGRGFRKKIVVFPGKNLDNELAGNVTDLCPVGALLNKDNLHEERVWYW"
     gene            972..>2178
                     /locus_tag="VFJ67_04010"
     CDS             972..>2178
                     /locus_tag="VFJ67_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289581.1"
                     /translation="MRSKVLQPIAIFLLLLALLSALIIFIIQTRYFRQFVKLTTNAVV
                     STLTAQNFTIGSIEGNFLEGITLKNVSLGIGGDKFIDCDEIYIDYSLPLILDGSMLFS
                     RVVPLNEVRVTGLRVNLVHHKDDTWNFQVIQKLMMKEKKPNPDWNFFIENGSIRDAVM
                     TIHDESRNERSIFELPDAHLSVNLFKIADKAEFNVRDGHLIAAYESMDFEKLHFENIK
                     GKAVYSNKVLPDRLEVENVVFNYMGATVTGKGAIDNMMNPTFNLAGEIKDVEIPEIGK
                     LIVEVEGRGSSAKWKDLHATGEMKLRSSSLLESPLGGGIGSVVIDNTHVALSKGSLSA
                     PFGQASFEGAIDLYGMKNRSKDNIADIEFDLKSLKVPAIFELIEKKNGPIEAGINRNL
                     DAVINSKFKV"
BASE COUNT          520 a          551 c          585 g          522 t
ORIGIN      
        1 ctgtcccttc ctcgggtcga ctccgaccgt aatgttgcac ccattgctgc agagcgagca
       61 aacgctgtcg gtgaattacc agtaccacac ccgctcctcg tgaaggttat ctttattgag
      121 gagcgcaccg acggggcaaa gatccgtgac gttacccgca agctcgttgt cgaggttttt
      181 tcccgggaag acgactatct ttttcctgaa ccccctcccg tcgaatccca gctcgcctgt
      241 tttcgtgacc tcgtccgtga acctgacgca cctcgtgcac atgatgcagc ggttcgggtc
      301 gtataatatc ctgtcgctga ggtagtattt ccttctgacc ttcttctctt cgggcccttc
      361 gaacctgctg tagccgggcc cgtgttttac tgtcgtgagc tggagagggc attcgccgcc
      421 cttatcgcat ataggacagt cgaggggatg gttgatgagc gtgaactcta cagtcgcctt
      481 cctcgccttg atagcttttt cggtattggt tttgactatg aggccttctc tcacgaacgt
      541 attgcaagcg ggcatgacct tcggcacgcc ctccacctcg accaggcact gcctgcactg
      601 tgcgactatg ctcagggccg ggtggtagca gaaatggggc acgtcgatgc cgacgcggga
      661 agccgcctgt atgagattga ggccgtcttc tacctctact tcgatcccgt ctatagtgag
      721 cttaggcata ggaaattcgg ttaagcgaga gtcgggaaaa tattatctga cgggcggtgc
      781 cttagcaagt tatcggggga ataaggggtg attgcagcag ccgcggattt acgcccggct
      841 gaggctgttt ataagtcgta gcgggatgtt ctgataatgc ctgagcgcat ccgtaacacc
      901 tcgggccgga cgctcctgcc cgaaagttat ctcgcccgga ggggcatgtg ttataattca
      961 cggtacaggc tatgcgttcg aaggttcttc aacctattgc tatattcctt ctgctactag
     1021 ctttattatc ggcattaatc atttttataa tccagactcg ttacttcagg cagttcgtca
     1081 agctcacgac gaacgcagtc gtcagcaccc ttaccgccca gaatttcacg atcggcagca
     1141 tcgagggcaa tttcctcgag gggatcacgc ttaagaatgt ctccctcggc ataggcggag
     1201 ataaatttat cgactgcgac gagatataca tcgattattc gcttccgctc atcctcgacg
     1261 gctcgatgct tttcagcagg gtggtccccc tcaacgaagt cagagtcact ggtctcaggg
     1321 taaacctcgt ccaccacaag gacgatacgt ggaatttcca ggtaatccag aaattaatga
     1381 tgaaagaaaa aaagcccaat cccgactgga acttcttcat agagaacggt tcgatcaggg
     1441 acgctgtaat gacgatccac gacgagtcga ggaatgaaag gtcgatcttc gagctgccgg
     1501 atgcgcatct gtcggtcaac ctgttcaaga tagccgataa ggccgagttc aatgtcaggg
     1561 acgggcacct gatcgcagcc tacgagagca tggatttcga gaagctgcat ttcgagaaca
     1621 taaagggcaa agccgtctat tccaataagg tgcttcctga caggctcgag gtcgagaacg
     1681 tcgtattcaa ttacatgggc gctaccgtta ccggaaaagg ggcgattgac aatatgatga
     1741 accccacttt caacctcgcg ggagagataa aggatgtcga aatacctgag atagggaagc
     1801 tcatcgtaga ggtcgaagga cggggaagct ccgctaagtg gaaggacctg catgctacgg
     1861 gagagatgaa gctcaggagc tcgtctcttc tcgaaagccc actgggaggc ggtatcggat
     1921 cggtcgttat cgacaacacg cacgttgcat tgagtaaggg gagcttgagc gcgcccttcg
     1981 ggcaggcgtc gtttgaaggc gcgatcgacc tctacgggat gaaaaacagg agtaaagata
     2041 atattgcgga tatagagttc gatttgaagt ctctcaaggt tcccgctata ttcgagctta
     2101 tcgagaaaaa aaacggacct atcgaagcgg gaatcaaccg aaacctcgat gccgtaataa
     2161 attcgaaatt taaggtta
//
LOCUS       DASSJH010000188         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_906140, whole genome shotgun sequence.
ACCESSION   DASSJH010000188 DASSJH010000000
VERSION     DASSJH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906140"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VFJ67_04015"
     CDS             <1..1003
                     /locus_tag="VFJ67_04015"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET7289582.1"
                     /translation="IELAKLIVKSFPSIDMVRMTSSGTEATMSGVRLARAYTKRDYII
                     KFEGCYHGHADYLLVQAGSGATTFGTPSSPGVPKSFTEKTLLANYNDLGSVMRLYDKY
                     GDRIACVILEPVAANMGVVPPAYGFLEGLRKVTRDSGSLLIFDEVITGFRLGMGGAQR
                     KYGITPDITCLGKIIGGGLPVGAFGGRKEIMSMVAPLGPMYQAGTLSGNPLAMAAGLA
                     TLRKLKERGNYKRLRDLSRMMVEGINEVIKKAGVTAVVNSVDSMFTLFFAGAAPVDYR
                     TVLKSDTKKYSKFFENLLRAGIMFPPSQFEAVFLSLAHTERDIDRTLDAVYKALKTV"
     gene            1055..1255
                     /locus_tag="VFJ67_04020"
     CDS             1055..1255
                     /locus_tag="VFJ67_04020"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET7289583.1"
                     /translation="MFIAIGFELGFSVIAGILLGQYIDKLVGTRTPWFTIIGLLAGGF
                     AGFNILLRMIKRVNDGKDSESD"
     gene            1227..1613
                     /locus_tag="VFJ67_04025"
     CDS             1227..1613
                     /locus_tag="VFJ67_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HET7289584.1"
                     /translation="MTERTASLINLPSIGRVERTGLAVTGVLMVVSYFLGSRELAVGV
                     AAGGILFTANFVAIRFLVHALVGKSHTKGFGIFAFVIKMAVLIAIVAALFIFAKVNLY
                     GFFIGVTGVVIVIIGESLRGSKDGTL"
     gene            1600..2298
                     /gene="atpB"
                     /locus_tag="VFJ67_04030"
     CDS             1600..2298
                     /gene="atpB"
                     /locus_tag="VFJ67_04030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET7289585.1"
                     /translation="MEHFSWFTLIGLGQYDYILGSILVLILLALAGFSIKSALSKEDS
                     VLPDEKFSIRTIFEILTIDFLFDLFTNMLGSKENAKKYFPLLGASFFFILFANFLGII
                     PGFLPPTGNFNVPVACALVIFVMYNFYGFKENGLAYLKHFAGPVIFLAPLMFIIEVIS
                     HFVRPVSLSLRLFMNITGDHLILGVFTNLVHYIIPAIFIGLGIFVSFLQAFIFTVLSA
                     IYISLATAHEAEIE"
     gene            2353..2679
                     /locus_tag="VFJ67_04035"
     CDS             2353..2679
                     /locus_tag="VFJ67_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET7289586.1"
                     /translation="MKTALSILSIVGVAILASFIPDAAFAQEGSGGELTKLGLALGAG
                     LGIGIAAGVAGLGQGRATAAALSGIARNPGASSKILTPMIIGLALIESLVIYALLIAF
                     LLQGKI"
     gene            2756..>3048
                     /locus_tag="VFJ67_04040"
     CDS             2756..>3048
                     /locus_tag="VFJ67_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289587.1"
                     /translation="MKKAAACLLIVFVLFGCGKTAGLIQKDDQESLAKSSDLYYKLIM
                     WKYYEKAAQFVDPDKHKEFEAFVLANQKDLNITGYEIKEMVYLTENEAGTEPA"
BASE COUNT          710 a          727 c          826 g          785 t
ORIGIN      
        1 aatagagctg gcgaagctca tagtcaagtc ctttccatcc atcgacatgg tcaggatgac
       61 cagctcgggt acggaagcca cgatgagcgg ggtgcgtctc gcgagggcct atacgaagag
      121 ggactacata attaagttcg aagggtgcta ccacgggcat gccgattacc tcctcgtcca
      181 ggcgggatcc ggggccacca ccttcggaac gccgagcagc ccgggtgttc ccaaatcctt
      241 tacggaaaag actctgctgg ccaattataa cgacctcggc tctgtgatga ggctgtatga
      301 taaatacggc gacaggatcg cgtgtgtcat actcgagccg gtcgctgcca atatgggcgt
      361 ggttccgccg gcttatggat ttcttgaggg attaaggaag gttacccgcg attcaggttc
      421 actcctcatc tttgacgagg taatcacagg gttcaggctc ggtatgggcg gagcccagcg
      481 caaatacggc attacgcccg acatcacctg cctcgggaaa attataggtg gcgggctccc
      541 ggtgggggcc ttcggcggaa ggaaggaaat tatgagtatg gtcgcccctt tgggacccat
      601 gtaccaggca ggcaccctgt ccggaaaccc gcttgccatg gcggccggtc tcgcgaccct
      661 gagaaagcta aaggagcggg ggaattacaa gaggctgcgt gatctctcgc gtatgatggt
      721 cgaagggata aatgaagtaa taaagaaggc aggcgttacg gctgtggtca attcggtcga
      781 ttctatgttc acgctctttt ttgccggggc cgccccggtc gattaccgga cggttctcaa
      841 gagcgataca aaaaagtact ccaaattttt tgaaaattta ctcagggctg gtattatgtt
      901 tccgccctct cagtttgagg ctgttttcct ttcactcgcc cacactgaaa gggacatcga
      961 tcgtacgctc gatgccgtgt ataaagctct taaaacggtc tgattttaca ggggggaatg
     1021 tacctttaag agtaacggaa aatataaatg gctgatgttt atcgccatcg ggttcgagct
     1081 cgggttttcc gtgatcgcgg gcatattgct ggggcagtat attgataaac tcgttgggac
     1141 ccggactccc tggtttacga taatcgggtt actggcgggg ggatttgcgg gatttaatat
     1201 tctcctgagg atgataaaaa gggttaatga cggaaaggac agcgagtctg attaatttgc
     1261 cgagcatagg acgggtcgaa agaaccgggt tggctgtaac cggagttctt atggtcgtgt
     1321 cttattttct cggctcgagg gagcttgcgg ttggagtcgc ggcgggggga atactgttta
     1381 ccgccaattt cgtagccatt aggttcctcg tgcatgcgct agtggggaaa tcgcatacta
     1441 aggggttcgg aatattcgcg ttcgtaatca agatggcggt cttaatcgcg atcgtcgcgg
     1501 ctttgttcat tttcgccaaa gtgaacctgt acggattttt tatcggggtg accggggtcg
     1561 ttatcgttat aataggagaa agcttgagag ggagtaaaga tggaacactt tagctggttt
     1621 actttaatcg gcctcggtca atacgactat atattggggt cgatcctcgt gcttatcctt
     1681 ctcgcactgg ccgggttctc gataaagagc gccctctcaa aagaagattc ggtattgccc
     1741 gatgagaaat tctctataag gactatcttc gaaatcctca ctatagactt tctattcgat
     1801 ctcttcacga acatgctcgg ctcgaaagag aatgcgaaga aatattttcc gctcctggga
     1861 gcctcgtttt tcttcattct gttcgcgaac tttttaggca taatccccgg tttccttccg
     1921 ccgacgggga acttcaacgt gcccgtagcc tgcgcgctgg tcatattcgt tatgtacaac
     1981 ttttacgggt ttaaggaaaa cgggctcgct tatctgaaac atttcgcagg gcccgtgatc
     2041 ttcctcgctc cgctaatgtt cattatcgag gtaataagcc atttcgtgcg cccggtatct
     2101 ctctcgctca ggcttttcat gaacattacc ggggatcacc tcatactcgg cgttttcacg
     2161 aatctggtgc attacataat acccgctata ttcataggcc tcggaatatt tgtatctttc
     2221 ctgcaggcgt ttatatttac ggtgctttcg gcgatataca tatcgctcgc gacggcgcat
     2281 gaagctgaaa tcgaataaga tcgaacacta aatttgaaaa caaatgacct actatacaaa
     2341 aggaggtaaa aaatgaaaac agcactttcg attctctcga tagtcggagt cgccatcctc
     2401 gcttcattta ttcccgatgc ggctttcgcg caggaaggga gcggcggaga gctcacgaag
     2461 ctcggactcg cccttggcgc gggattgggg attggtatcg cggcgggtgt ggccggtctg
     2521 ggtcaggggc gcgcgactgc ggctgcattg agcggcatcg cgagaaaccc gggcgcaagc
     2581 tccaagatcc tcactccgat gattatcggt cttgcactca tcgagtctct cgttatctat
     2641 gctcttctca tagcattcct gcttcaggga aaaatataat tcggctgcgg gggcctatcg
     2701 gtcccccgca ttcgtttatt tgaatgtttc ttccgcaggc attaacattt ctactatgaa
     2761 gaaagcagcg gcttgtttac taatcgtctt cgtcctcttc ggctgcggta aaacggccgg
     2821 actgatacag aaggacgatc aggagtccct tgcaaaatcg agcgacctct attacaagct
     2881 cataatgtgg aagtactacg agaaggccgc gcagttcgtt gatcccgaca agcacaagga
     2941 gttcgaagct ttcgtgctcg ccaaccagaa agacctcaat atcaccggat acgagattaa
     3001 ggaaatggtc tatcttacgg aaaacgaggc cgggacggag ccggcgca
//
LOCUS       DASSJH010000189         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1557884, whole genome shotgun sequence.
ACCESSION   DASSJH010000189 DASSJH010000000
VERSION     DASSJH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557884"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFJ67_04045"
     CDS             <1..925
                     /locus_tag="VFJ67_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289588.1"
                     /translation="WRFDISMPIEIDGSLSESGPEGNLGLVADDWSGGIAFCANTNGE
                     CFDAQDQPTVPNTNIEPIYFAPTIVLDDLGRRHVIFVTGDRRDPSSILKSGKLYNFID
                     DYIPAYLAGGTPTGGNVIKTASTLISAGQVIELTAQSGVEGQFVSTASNNFSSDQGEF
                     LVKFPSNLGNPELGEKGFGVPVVINRVLIFTTYAPELDSSDPCSGGTGLGRIFAIDFI
                     TGSAALSRIPGVRESDILKGSSAQQGLAAGSTVAEGMPTPAQLTFGARGSVLMSVAFT
                     GGPVAGGSQFIVWELPPLPTRTQTLFWEELL"
     gene            940..1596
                     /locus_tag="VFJ67_04050"
     CDS             940..1596
                     /locus_tag="VFJ67_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7289589.1"
                     /translation="MRLARNTNGFSLIEVMVVVTIMVMLLAIGTSILGGRAAQRRSVD
                     EVTNNVSSMLQTGKLMSVRDGVEYRVVFARCDDVDDTDPDCPKCDEYVEYQAGDKDIS
                     LIMERGDSNTGSTVWCMQSEHSKRFQSDLDITTSENLGEDGQPLEFAFVPTGMRRDFG
                     TDVNNETLTISPAVDSNIENCGQLRVSPAGGVSVTEGRWDGSQCNPILDSSASPTPAP
                     "
     gene            complement(1708..2787)
                     /locus_tag="VFJ67_04055"
     CDS             complement(1708..2787)
                     /locus_tag="VFJ67_04055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7289590.1"
                     /translation="MCSACNTGLNQAKADQFAEKMFNVLNHGALTIMISIGHRTGLFD
                     AMSGLTHSTSLEIANAAGLNERYVREWLGAMVTGGIVEYDPDEGTYHLPAEHAVWLTR
                     KAAPNNFAVSTQWFSIAGSVEDLIVECFKNGGGVPYEEYKRFNEVMSEESSQTVVFPL
                     RDHLLPLVPGINERLEAGIDVLDVGCGSGYALIHMAKMFPNSRFMGYDLLPEAIERGR
                     TSARESGLTNITFESLDVSRLQDRERFDLITTFDAVHDQADPARVLSNIHAALKDDGV
                     YFMQDIKGSSHVEKNMDNPLAPFLYTVSCTHCMTVSLAQGGKGLGAMWGKELACEMLK
                     EAGFGEIDVKELPHDPINYYYIIRK"
     gene            complement(2845..>3089)
                     /locus_tag="VFJ67_04060"
     CDS             complement(2845..>3089)
                     /locus_tag="VFJ67_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289591.1"
                     /translation="TEPEHPIRKEVKLHEDRFRSILKDVVRDLKNSDPGFKSLNVDNV
                     VDTYYLILEGAIVASKNYNDTWPLDRAVEAVEKLIE"
BASE COUNT          679 a          819 c          848 g          743 t
ORIGIN      
        1 gtggaggttc gatatatcca tgccgatcga aatcgacggt agcttatccg aaagcggtcc
       61 tgaagggaac ctcggtctcg ttgcggatga ctggtccggc ggcatagcgt tttgcgcgaa
      121 taccaacggt gaatgtttcg acgcgcagga tcaaccgacg gtccccaata ccaatataga
      181 gccgatttac ttcgcaccga ccatcgttct cgacgacctc ggaaggagac acgtcatttt
      241 cgtaacgggt gacaggagag acccgagcag cattctgaaa tccgggaagc tctataactt
      301 tatcgacgac tatataccgg cgtatctcgc gggagggact cccacagggg ggaatgtgat
      361 aaaaactgca tcaacgttga tctccgcggg gcaggtcatc gagcttacag cgcaatccgg
      421 tgtagagggg cagttcgttt caacggcatc caacaatttc agctcggacc agggagaatt
      481 tctggttaaa ttcccgagca acctcggtaa tcccgagctc ggggagaagg gtttcggcgt
      541 gccggtggta attaacaggg tcctcatatt tacaacatac gctcccgagc tcgattcatc
      601 ggatccatgc agcggaggaa cgggcctggg aaggatattt gccatcgatt tcataaccgg
      661 gtccgcagcg ctctctcgca tacccggggt tagagaaagc gatatattaa agggctcgag
      721 cgcgcagcag ggtttggcag cgggctctac ggttgcggag ggaatgccga ctccggcaca
      781 gcttactttc ggggcgaggg ggtcggtact catgtccgtg gctttcacgg gaggccctgt
      841 ggccggcggg tcccagttca tagtgtggga gctcccgcct cttccgacga ggactcaaac
      901 gctgttctgg gaggagctgc tctaggggta aaaagatcaa tgagactagc gcggaataca
      961 aacgggttca gccttatcga ggtgatggtc gtcgttacta tcatggtaat gctgcttgct
     1021 ataggtacgt cgattttggg cgggagggcg gcacagcgcc gctccgtgga cgaggtcact
     1081 aacaacgtgt cttccatgct tcagaccggg aaattgatgt cggtaaggga cggggtcgaa
     1141 tacagggtcg ttttcgcgag atgcgacgac gtagacgata cggatcctga ctgtccgaaa
     1201 tgtgacgaat acgtcgagta tcaggccggg gataaggaca tttcgctcat catggagaga
     1261 ggggacagca acacgggctc cacggtatgg tgtatgcagt cggagcactc gaaaagattt
     1321 caatcagatc ttgatatcac aacctcggaa aacctcggcg aggatggaca accccttgaa
     1381 tttgcctttg ttcccacggg catgaggaga gatttcggga cggacgtaaa taacgagact
     1441 cttacgataa gtcccgcggt cgattccaat atagagaact gcggacagct aagggtatct
     1501 cccgcaggag gcgtatcagt aacggaaggc agatgggacg gctcgcaatg caatccgatt
     1561 ctcgattcgt cagcttcacc cacgcccgct ccgtagtgaa ggtctcgagc cgcatcagta
     1621 tttcctcgat gatatgcata caacaccatt gtaacattcg gttcgtcttc ctttctctac
     1681 ggcctgccta taggctgtgt tctcagctca cttcctgatt atgtagtagt agttgatcgg
     1741 gtcgtgcggc agctctttca catctatctc gccgaaccct gcttccttca gcatttcgca
     1801 tgcgagctcc ttcccccaca tcgcgcccag tcccttcccg ccctgagcaa gcgataccgt
     1861 catgcaatgc gtgcaggata ctgtatagag gaacggagcg agcgggttgt ccatgttctt
     1921 ctcgacgtgg ctcgagccct tgatatcctg catgaaataa acgccgtcgt ctttcaacgc
     1981 cgcatgtatg ttggacagca cgcgtgcggg atcggcctga tcgtgcactg cgtcgaaggt
     2041 cgttatgagg tcgaacctct ccctgtcttg caatctggaa acgtccagag actcgaatgt
     2101 gatattggtc aaaccgctct ccctcgcgct cgttcttccc ctctctatag cttccggcag
     2161 gaggtcatat cccatgaacc tgctgttcgg gaacatcttc gccatatgga tgagcgcata
     2221 accgctcccg cagccgacgt cgagcacatc gattccggcc tctagccttt cattgattcc
     2281 ggggacgagc gggaggaggt ggtcgcggag agggaagacg accgtctggc tgctttcctc
     2341 ggacataact tcgttgaagc gtttatattc ttcatacgga acgccgccgc catttttaaa
     2401 gcactcgaca atgaggtctt cgaccgaacc cgctatggag aaccattggg tggatacggc
     2461 gaagttattg ggcgcggctt tcctcgtgag ccagactgcg tgctcggcgg ggaggtgata
     2521 cgtcccttcg tccggatcgt actccacgat gcctcccgtg accatagcgc ccagccattc
     2581 tcgcacatac ctctcgttta gccccgccgc attcgcgatc tccaagctcg ttgaatgcgt
     2641 aagcccgctc attgcatcga acaggcctgt cctgtgcccg atggatatca ttatcgtaag
     2701 ggctccgtgg ttgaggacgt tgaacatctt ttccgcaaac tggtcggctt tcgcctggtt
     2761 taaacctgta ttacatgcgc tgcacatatt aaagctcctc ctgattgtta gacagacagg
     2821 tctgtcttta tatagttaaa aattttattc tataagcttt tcgacagcct ctacagcgcg
     2881 atcaagcggc caggtatcgt tgtaattctt gcttgcgaca atggccccct cgaggatcag
     2941 atagtaagta tccacgacat tatcgacgtt caggctttta aatcccgggt ccgaattctt
     3001 aaggtcccgc actacgtcct tcaggatgga gcggaatctg tcttcgtgga gcttgacctc
     3061 ctttcgtatc ggatgttcgg gctcggtta
//
LOCUS       DASSJH010000190         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1232641, whole genome shotgun sequence.
ACCESSION   DASSJH010000190 DASSJH010000000
VERSION     DASSJH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232641"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFJ67_04065"
     CDS             <1..353
                     /locus_tag="VFJ67_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7289592.1"
                     /translation="EKRKKKKDEGFLYVRSGEGQWVELMKGVKVKNLYRDPKKNYATL
                     LVRMDPGTTFPDHIHTDTEECYVIDGDLRMGGQIFGKGDYIRAEAHSTHENISTDNGC
                     FLLIAASEENELLA"
     gene            765..1334
                     /locus_tag="VFJ67_04070"
     CDS             765..1334
                     /locus_tag="VFJ67_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003854991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET7289593.1"
                     /translation="MHKVIKKYLMLPAAAVIFSVAGLSAFADTTVMVGGEAMYPTKDI
                     VDNAVNSADHTTLVAAVKAAGLVETLKGPGPFTVFAPTNDAFENLPDGTVEMLLKPEN
                     KDALTKVLTYHVVPGHLTFDALAQQIKAGNGKAELKTVSGGTLTVWMNGPHNILIKDE
                     KGDIADISTYDVIQSNGVIQVIDTVLLPN"
     gene            1414..1950
                     /gene="msrB"
                     /locus_tag="VFJ67_04075"
     CDS             1414..1950
                     /gene="msrB"
                     /locus_tag="VFJ67_04075"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7289594.1"
                     /translation="MSTKAVITIIISVLIIAGFMFIVLNKADTRGDNQSREVNASNTS
                     ADTLPPGISGPTKEEWMKKLTPQQYYILWEKGTEKAFSGSLLNNKKKGIYVTAGCKLP
                     VFSSENKYDSGTGWPSFWKPISDDSVILKPDNDLGFERTEVLSKCGEHLGHVFKDGPQ
                     PTGLRYCINSAALEFIEE"
     gene            1991..>3574
                     /locus_tag="VFJ67_04080"
     CDS             1991..>3574
                     /locus_tag="VFJ67_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HET7289595.1"
                     /translation="MGLSRVLTVFTYLLAVLSVSLAFNYTAALAANHREAPITAIDDE
                     ADITDVFAFVSYDPDDPSASDKVTMIVNYDPFLEPGNGPNYFPFDSEILYAIRVDNDQ
                     DALEDIVFEFRFTTEIRLPNVFTGFVGALDGINAPDNSPPDLDGNPTAGQPVIPPAIT
                     ALDGDGSQGLSLRQFYSVTMVKGNNRTQLTSATGSPLFAVPTNVGPRTMPDYPSLFAQ
                     GIYDLGSGIKVFAGTIDDPFWLDLGATFDSINFRTQGFVVQGVLTDEQDADGSNNYAP
                     DDFSGYNVNTIAIEVPIELLTSGGEKHAASDPEAVIGMWGTSSRPKEKKYRGPGEDPK
                     LSKKFTQIQRMGNPLFNELVIGTGFKDKFSMAQPENDAEYSDFFLDPVFARVLNTVYE
                     ALYGPDVLPIPDVPRLDLLPLVQYTAPIAPEGTPTGPIADVLRLNTGIPPTPPDKQSR
                     LGFLTLLDDDPSNDDPAGFPNGRRVVDDVTDIAARVVAGVLVPEFNNFPHNRIGDGVN
                     VNDVQPYNTSFPYLNYAHDG"
BASE COUNT          937 a          924 c          928 g          785 t
ORIGIN      
        1 aagaaaaacg caaaaagaag aaggatgaag gtttcctgta cgttcgaagc ggcgaagggc
       61 agtgggtcga actcatgaaa ggggtcaagg tgaagaacct gtacagggac ccgaaaaaga
      121 attacgccac gcttctcgtc aggatggacc cggggacgac gtttcccgac catatccata
      181 ccgataccga agagtgctac gtcatagacg gcgacctcag gatgggaggt caaatattcg
      241 gaaaaggcga ctatatccgc gccgaagctc acagcactca cgagaacata tcgaccgaca
      301 acggctgctt cctgctcatc gcggcgtccg aagaaaacga gcttctcgcg taatccggcc
      361 tcataaacgc ataaagcccc ctgccggatt acgcttagct acatgacacg agcgattgaa
      421 cgagcaggta atttctcgag cgtaatcata tccggtaaat ctaaattaaa agtgatccgt
      481 aatctgtttt cttccgtata acaattaggg cggcactgtt attagcgccg cacgatacag
      541 ccaatacctc gacccgcatc ccgattccgc atcggtattc cgtttttgat gatccgagct
      601 ttatccttct atatatactg tactgcctac atattatata tatctatatc gtccggcctt
      661 tgatgcttat cccttaaatc gataaatgtt tcatgaagtc gagatctaaa aattttttcc
      721 ctccgtataa atattcaaat tacaaacttc aaggaggtgc agaaatgcac aaggttataa
      781 aaaagtattt aatgctgcct gcggcagcgg taattttttc ggttgcaggg ttgtcggcgt
      841 ttgcagatac aaccgtaatg gtcggcggag aggctatgta tccgacgaag gacatagtgg
      901 ataacgcggt caattcggcc gatcacacaa ccctcgttgc ggcggtaaag gcagcggggc
      961 tagtcgagac actgaaggga ccgggcccgt tcaccgtgtt cgcaccgacc aacgacgctt
     1021 tcgaaaacct tcccgacggt acggtcgaga tgcttctcaa gccggagaac aaggacgctc
     1081 tcacgaaagt gctcacttat catgtagtcc ccggccacct caccttcgac gcgctcgccc
     1141 agcagataaa ggcaggcaac ggcaaggccg agctcaagac ggtaagcggc ggaacgctga
     1201 ccgtgtggat gaacggtccc cataacatcc ttattaagga cgagaagggc gacatcgcgg
     1261 atatatccac atacgacgta attcagtcaa acggagtcat acaggtaata gatacagtac
     1321 tgctcccgaa ctgagctgat tcgatcattt gctgagaggg cgctttcaac ccgcatgttg
     1381 gcgccctcat accggcaagc acggagatca ctaatgagca caaaagcagt aatcactatc
     1441 ataatttcag ttcttatcat agccggcttt atgttcatcg ttttaaacaa agccgataca
     1501 cgcggggata atcaatcccg cgaagttaat gcgtccaaca cctcagccga tacgctccct
     1561 ccgggtatat cgggtccgac caaggaagaa tggatgaaga agctcacgcc ccagcaatat
     1621 tacatcttgt gggaaaaggg tacggagaag gcattttcgg gctcattgtt aaacaataag
     1681 aagaagggga tctacgttac ggcaggctgc aagctcccgg ttttcagctc cgaaaataaa
     1741 tacgactccg gtacaggctg gccgagtttc tggaagccga tttcggatga ctccgtgatc
     1801 ctgaaaccgg acaacgatct ggggttcgag cgtacggagg tgctcagtaa atgcggcgag
     1861 catttgggac acgtctttaa agacggcccg cagccgaccg ggctcaggta ctgcatcaac
     1921 tcggcggcgc ttgagttcat tgaggagtaa agagtagaag acaaaaacaa tacatttagg
     1981 aggtgttatc atgggattat cgagagtctt aaccgttttt acatatctgc tggcggtatt
     2041 atcagtttct ttagcattta attacacagc cgctctcgcg gctaaccaca gggaagcgcc
     2101 gataacggcg atcgacgatg aagcggatat cacggacgtt ttcgctttcg tcagctacga
     2161 tccggatgat cccagcgctt ccgacaaggt cacgatgata gttaactatg atccttttct
     2221 ggagcccgga aacggtccta actattttcc gttcgactcc gagattctct atgcgataag
     2281 ggtcgataac gatcaggacg cgctcgagga catagttttc gagttccgtt tcactacgga
     2341 gataaggctt ccaaatgtgt tcacgggttt cgtaggcgcg ctcgacggaa tcaatgctcc
     2401 tgacaattca ccgccggacc tggacgggaa cccgacagca gggcagccgg tcataccgcc
     2461 tgcgatcacc gccctcgacg gagatggttc gcaaggattg agcctgagac agttttacag
     2521 cgttacgatg gtaaaaggca ataaccggac gcagctcaca agcgcgacgg ggagtccgct
     2581 ctttgccgta cctacgaatg tcggtccgag gaccatgcct gactatcctt cgcttttcgc
     2641 gcaggggata tacgatctcg gcagcggaat caaggttttc gcgggaacga tcgacgaccc
     2701 gttctggctc gatctcggtg cgactttcga ctcgataaac ttcagaaccc agggcttcgt
     2761 cgtccagggc gtgctcactg atgaacagga cgcggacggt tcgaacaact acgcgcctga
     2821 cgatttctcg ggttataacg taaatacgat agcaatagag gtgccgattg agctcctgac
     2881 gagcggcgga gaaaagcacg cggcatccga ccccgaggcc gtaatcggca tgtggggcac
     2941 gagctcgagg ccgaaggaga aaaagtacag ggggccggga gaagacccga agctgtcgaa
     3001 gaaattcaca caaatacaga gaatgggcaa ccccctcttc aacgagctcg ttataggtac
     3061 gggcttcaaa gataaattca gtatggcgca gccggagaac gacgcggaat attcggattt
     3121 cttcctcgat cccgtatttg cgagagtgct caataccgta tacgaagccc tatacggccc
     3181 tgatgttctc cccataccgg atgtcccgag attggacctg ttgcccctgg tacaatacac
     3241 ggctcctata gctccggagg ggactccgac gggtcctata gcggatgtac tgagactgaa
     3301 caccggtatt cctcccacgc cgcccgataa gcagagcaga ctcggattcc tgacgctcct
     3361 cgatgacgat ccgtcaaacg acgaccctgc ggggtttccg aacggacgcc gcgtcgttga
     3421 cgatgttact gatatcgctg cgcgtgtcgt agccggtgtg ctggttcccg aattcaataa
     3481 ttttccgcac aacaggatcg gggacggcgt caacgtgaac gacgtacagc cgtacaacac
     3541 gtcattccct tatttaaatt atgctcacga cggg
//
LOCUS       DASSJH010000191         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1124584, whole genome shotgun sequence.
ACCESSION   DASSJH010000191 DASSJH010000000
VERSION     DASSJH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124584"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="VFJ67_04085"
     CDS             complement(<1..150)
                     /locus_tag="VFJ67_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET7289596.1"
                     /translation="MDTVRLGLAQINVRVGDVEGNLKKILGYIKDARAGGVDILSFPE
                     LAVTGY"
     gene            complement(301..909)
                     /locus_tag="VFJ67_04090"
     CDS             complement(301..909)
                     /locus_tag="VFJ67_04090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7289597.1"
                     /translation="MERSQNLRNCLFPLAIYAVIICLIFLMKVFLPWQFVVSLSALLM
                     LSVPLVLKSDTSDLRWDPKGLLVGILASAALLLIYIAVLYGYGRYSGKSLVFNGLSYP
                     FILVQLMLVALPEEVFFRGYMQQKLGNTIKGVIAVSFLFALAHFVTLCLADGGFSVCG
                     QAVLTFFPSLVMGYLYMATGTLWASIIFHFLANIVHISAGFS"
     gene            complement(965..2197)
                     /locus_tag="VFJ67_04095"
     CDS             complement(965..2197)
                     /locus_tag="VFJ67_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289598.1"
                     /translation="MNICTEAYKKYSYLVHAVLLGLAGYIVYFLTQPRQHDYFNYFSH
                     LADAFLHGRLYLTDPPSTFEELIAVGNKYFEIYPPMPAILLMPFVAVLGVSFDHVLVS
                     FFVGGFNLAVVYLIMRKLTEDEEMRIWMTVLFGFGTIHWYLATVGSVWYFAQIVATAF
                     LLCAVLVTFTIRKPLLIGLLVGAAYWSRLPTLLSLPFFVIMLSDIWLLPKNGAGILKR
                     VRLSPLLLLGAGVGIFVLLNFLYNYLRFGSVFDVAYVKQSIYPAKENISPWFNKGLFD
                     LSYIPYHLRVLFLEPPVFMNTWPYVIPSKIGLSILITTPAFIFALFAGIRNKLSLACW
                     SAILTTAFLIFIKSGTGWTQFGYRYALDFYPFLLLLTLRGIGRELKWYHKALIIAGVL
                     VNLWGVLFINKFEWFKAY"
     gene            2321..>2977
                     /locus_tag="VFJ67_04100"
     CDS             2321..>2977
                     /locus_tag="VFJ67_04100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7289599.1"
                     /translation="MKFNPQVKDLSLIAVISIVWTFVLAYIAYTIIEDKNPGSAISIW
                     NVWDTQHYLKIAEYGYSSSTADKRNLLIVFFPLYPYLIKVFALVFKNYLLSALIVSNL
                     AYAATAFYLYKLVRLDFEDDDAYRSVIYISVFPTAYFLHAAYTESLFLALAIACFYYA
                     RKENWPLAGVLGMLAAATRVTGILLLPVLIIEYLHQKGYKLKNIKINILWIFVIALGL
                     V"
BASE COUNT          846 a          731 c          752 g          648 t
ORIGIN      
        1 atagcctgtg acggcgagct cggggaagga gagaatatcc actcccccgg ccctcgcgtc
       61 tttaatgtat ccgagtatct tttttagatt cccttccaca tcgccgacac gcacgtttat
      121 ctgtgctagt cctaatctta cagtatccat cgtcaaaccc gtgtataaat tatagcactc
      181 taagaaataa aatgattaca gtattttaac cgaatcggga ccaagatgga atattccggg
      241 catgaaaaac ggacgcgggt tttcccgatt ccggcatatg atcgccgccc gacggacggt
      301 ttacgaaaat cccgccgata tgtggactat gttggcgagg aaatgaaaga ttatgctcgc
      361 ccagagcgtt ccagtcgcca tgtacagata acccatgacg agagaaggga aaaaagtgag
      421 gaccgcctga ccgcagacgg aaaaaccgcc gtccgccagg caaagggtta cgaaatgggc
      481 gagcgcgaag agaaagctta cggctataac gccttttatc gtattcccga gcttctgctg
      541 catgtagcct ctgaaaaata cctcctcagg cagagctacg agcataagct ggacgagtat
      601 gaagggatag gagagcccgt tgaagacgag ggacttcccg gagtaccggc cgtaaccgta
      661 aaggacagcg atatatatca gcagtagcgc ggccgaggcc aggatgccga cgagcagacc
      721 cttcggatcc cacctgaggt cgctcgtgtc ggacttgagt actaacggga cggacagcat
      781 caggagcgcc gacagtgaga ccacgaattg ccaaggtaaa aacaccttca tcagaaatat
      841 caggcatatt atgacggcat aaatagctaa cggaaacagg cagttacgga gattctggga
      901 tctttccata taagggagcc ggatgagatt ctagactcta aaatacataa cgcccgatat
      961 gcggtcaata agccttgaac cattcgaact tattgataaa caatacaccc cacaggttga
     1021 ctagtacgcc cgcaataata agcgctttgt gataccattt gagctcccgg ccgatgcccc
     1081 tcaaagtcag cagcaaaagg aagggataga agtcgagagc gtacctgtag ccgaactggg
     1141 tccatcccgt accgctttta atgaatataa ggaacgccgt cgtcagtatg gccgaccagc
     1201 aggcaaggga gagcttgttc ctgatcccgg caaagagagc gaatatgaat gccggcgtcg
     1261 tgatgagtat tgagagtccg atctttgacg gtattacgta gggccatgtg ttcatgaaga
     1321 ccgggggctc gagaaagagg acgcggaggt ggtaagggat atacgacaaa tcgaacagac
     1381 ccttgttgaa ccagggcgat atattttcct tggcggggta tatagactgc ttgacataag
     1441 caacgtcgaa gacagagccg aacctgaggt agttgtacaa gaagttaagc agaacgaaga
     1501 taccgacacc cgcgcccagg agcaggagcg gacttagcct caccctcttg agaatccccg
     1561 cgccattttt tggaagaagc cagatgtcag agagcatgat cacgaagaag gggagcgata
     1621 ataaggtcgg cagtctcgac cagtacgcgg ctccgacgag gagccctatt aaaagcggtt
     1681 tacggattgt gaacgttacg agcacggcac aaagcagaaa cgcggtagcg acgatttgag
     1741 cgaagtacca cacggagccg accgtggcca gataccagtg tattgtgccg aagccgaaga
     1801 gcacggtcat ccatatcctc atctcctcgt cctcggtcag cttccgcatt atgagatata
     1861 cgaccgccag attgaagccg cccacaaaaa aagacacgag cacgtggtcg aacgagacac
     1921 ccagaacggc tacaaagggc atgagcagta tcgcgggcat aggagggtaa atctcgaaat
     1981 acttattgcc taccgcaatg agctcttcaa aggtcgatgg cgggtcagtg aggtatagcc
     2041 ttccgtgaag aaacgcgtcg gccaggtggc tgaaataatt gaagtaatcg tgctggcggg
     2101 gctgggtcag aaaatataca atatatccgg ccaggcccag gagaacggca tgaacgagat
     2161 acgagtattt tttataggct tctgtgcaga tattcattaa aaatattcgg atccgctatt
     2221 caatatttca taagattgga ttaacattat attaagatta ccgacaacca gtaaaaatca
     2281 acgagacctg agacccgaag ccgaacgaag ccggtaatac atgaaattta atccccaagt
     2341 taaagactta tccctcatcg cggtaatttc gatagtctgg acttttgtcc ttgcatatat
     2401 cgcttatacg atcatagagg ataaaaaccc gggctcggca atttcgatct ggaacgtatg
     2461 ggacacgcag cactatctca agatcgcgga atacggctat tcaagctcta ccgccgataa
     2521 aagaaacctc ctgatagtct tcttcccgct ctatccgtac ttaatcaagg tcttcgcgct
     2581 tgtattcaag aattatctcc tttccgccct tatcgtatcc aacctcgctt acgcggcgac
     2641 cgcattttac ctttacaagc tggtgagact cgatttcgag gacgacgacg cgtacaggtc
     2701 ggtcatatat atttccgtct tcccgaccgc atatttcctt cacgccgctt acaccgagag
     2761 ccttttcctg gccctggcaa tagcctgttt ctattacgcg agaaaggaga actggccgct
     2821 tgccggcgta ctggggatgc tggccgcagc gacacgggtc accgggatcc ttctcctgcc
     2881 ggtactcatt attgagtacc tgcatcagaa aggatacaaa ctcaaaaaca taaaaataaa
     2941 catcttatgg atattcgtga tcgcgctcgg cctggtt
//
LOCUS       DASSJH010000192         6141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_371685, whole genome shotgun sequence.
ACCESSION   DASSJH010000192 DASSJH010000000
VERSION     DASSJH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371685"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFJ67_04105"
     CDS             complement(<1..196)
                     /locus_tag="VFJ67_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289600.1"
                     /translation="MINTVDEFMEEFEKHKDKVEWYLHQPTPTSNFTDIRGKGVLCSP
                     LTVMCTPPVHYHLDFLDIDVD"
     gene            complement(447..1094)
                     /locus_tag="VFJ67_04110"
     CDS             complement(447..1094)
                     /locus_tag="VFJ67_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7289601.1"
                     /translation="MNGGFETGDFTGWIVIQEPGSAGDWFVYSGDVAPISPHFVLPPP
                     VGVFAAISDQLESSSQVLYQDIDVPAGFSIACSVIIYYENFAEGFVTASDLSYTTAPN
                     QQARIDIMDPSADAFDVGAGVLENLYQTNPGDPLTLGYTVLNFDLSQYAGSTVRFRVA
                     QVDTVSYFLFAVDDVRCGEIVRNIPTLGEWGMIAMAGALGLAGLIYSRRRRALAA"
     gene            1502..2056
                     /locus_tag="VFJ67_04115"
     CDS             1502..2056
                     /locus_tag="VFJ67_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289602.1"
                     /translation="MTRPILPVIALILAFAFYARGAEAPMPEGYGVVVDVDWPSQPGH
                     RRFNVGNGDVVTGRYGWYSPPGAIGPADYNVLIITKPISKARTYIVFEATNIDPEVLK
                     KKLGRLEGEIQDVCDLEGALKPGDMVHITTEDIHGLTKVELHFTRGEDQKHVARVSFI
                     NHLVHPIPETDPAYPYIPYVSEIE"
     gene            complement(2261..2698)
                     /locus_tag="VFJ67_04120"
     CDS             complement(2261..2698)
                     /locus_tag="VFJ67_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289603.1"
                     /translation="MKKLMTMFVTVMLFAFVGLGVMGSSAADQPAEMQYEQPATEGMQ
                     PALQGDSAMPAEGSQIIQGVITLVGKNQLNVKETATGIEHQIRIDKAQEKMLTTGYEI
                     TAQIDNGMLVSYTLGGVPPNVKEIVYTARNLPEENVLEQQKMF"
     gene            complement(3139..3639)
                     /locus_tag="VFJ67_04125"
     CDS             complement(3139..3639)
                     /locus_tag="VFJ67_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289604.1"
                     /translation="MKRILTYTICGLLIAGSSLGAYSYEGESPAVQEQDVYGSPNTDI
                     MVREFETQVERTRAANDPTEMERRQYIPQFMEGSSGTVIGTVTLVGDNEMRMVESGTG
                     IEHQIMITEAQQKQLTTGFNIEAELNNGRLVNFVEQGVPPDVEKIVYSAEDLPQDNIL
                     EQQKAF"
     gene            4028..5152
                     /locus_tag="VFJ67_04130"
     CDS             4028..5152
                     /locus_tag="VFJ67_04130"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HET7289605.1"
                     /translation="MKGTINYIKSGIKTNIFLSFALSLVFLSCGGSGNENRVDTNAVL
                     SDLAYKVILDDYQQLLDRGQDLESAIGTLSGDITPGNVEKARDAWKAAREPWESSEGF
                     LYGPVDTEGIDPSIDSWPVDTSGIQAIIGSPDDIDTQYAAQLDGTLKGYHTMEYLLFT
                     IGGDFVTAEETADYLASDPRLVEYLHAIIVDFNAQVGKLIVFWSPGESDYAGELATAG
                     QGSNVYPTLKAGLEEVVNGILGIAHEVGNEKLNVPFSEQAPVFVESRFSSNSITDFTN
                     NILSIDKIYFGRNGSLSLSDIVGGIDAPVNDDVRQAIDEAVAAINDIPEPFNESVLDP
                     ALAPVIQNAIDRVVELRLVLENEVYPIMFPGGNKPPVDET"
     gene            5198..>6141
                     /locus_tag="VFJ67_04135"
     CDS             5198..>6141
                     /locus_tag="VFJ67_04135"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET7289606.1"
                     /translation="MRREIIRGILVSVMSVIILTGCDGGESDGGGGIGGTDELMLGGD
                     TTVFDASSQAFNFPATGITEEGLIKHDQGDGAFDAIFVSSPSPVNPGLGPVFNNNSCQ
                     SCHANNGRGRPDDTLTSLLFRVSVPGESAVGGPNPVAGFGLQLQTRSTVGTGKEAGVT
                     IEYTETPGEYGDGTEYSLRAPVYTIVDPYIPIPVEILISPRIAPPVFGIGLLEAIPED
                     EILANADPADEDGDGISGRPNYVWDFEKGTQALGRFGWKANTPTAIQQAADAYNQDMG
                     VTSPLFPTESSAGQTQEDGLSDDPEINADTLDDTVFYI"
BASE COUNT         1488 a         1627 c         1691 g         1335 t
ORIGIN      
        1 tgtcgacgtc gatgtcgagg aagtcgaggt ggtagtggac gggcggcgtg cacatgaccg
       61 tgagcgggct gcagaggacg cccttccctc ttatgtccgt gaaattcgag gtcggggtcg
      121 gctggtggag gtaccactcg accttgtcct tgtgcttctc gaactcttcc atgaactcgt
      181 caacggtgtt tatcatgcgt tatttagtat gctaggcggg aatttaaatg caaattacgc
      241 ggcgttcatc cttcggcgag atccccccta cgcaaaagct ccgggggatc acccgacgtc
      301 tcatagccct tgggcgacgt gccgcaggca gggcaaacgg gatttgttga atgtatgagt
      361 gaaaggtgac acccccatcc taaccttccc ctatatgaag gggaaggaag tataaaaatg
      421 tcgaaaggtt aaaggttaac gtatcgctac gctgcgagag cgcgcctgcg gcgggagtag
      481 ataaggcccg cgagcccgag cgctccggcc atagcgatca tgccccactc tccgagggtc
      541 gggatatttc taactatctc accgcacctc acgtcgtcaa cggcgaaaag aaaataactt
      601 accgtatcca cctgggcaac cctaaacctg acagtgctgc ccgcgtactg agagagatcg
      661 aaattgagaa ccgtgtaccc gagtgttaaa gggtcgcctg gattcgtttg ataaaggttt
      721 tccaatacac ccgctcctac gtcgaaagcg tcggccgaag ggtccattat atcaattctg
      781 gcctgttgat tgggtgccgt cgtatatgac agatcggatg cagtaacgaa tccttccgca
      841 aaattttcat aataaattat cactgaacac gcgatcgaga atcctgccgg cacatctata
      901 tcctgataaa ggacctgaga gctcgactct agctggtcgg agatagcggc gaaaacgcct
      961 acaggcggag gaagaacaaa gtggggcgaa ataggagcaa cgtccccgct ataaacgaac
     1021 cagtcacccg cgctgcccgg ctcctgaatt acgatccatc ccgtgaaatc ccctgtttcg
     1081 aagccgccgt tcacgaaggg attgtcctga gccagagcgt tgccatgaat tgaatataaa
     1141 aggaaaaaaa ccgcaaaaaa tttgaaaaaa taatgcaatt tgcgcattta accatcctcc
     1201 ttagcggtac tatcagtcat tataacaaaa ttcagtgaaa tattacatag ttccaaaata
     1261 ttgcgatgac ttttggtgtg tgtgacggcc ccggcattgt tcgcttacac ggctccgacc
     1321 ggatccggga ggatgcaatc cgtgaagcgt tgcggaaaat gacagtccgg agggcgggtt
     1381 aggccgggtt aaggaccgaa tactctaaca attaaagcac tcaatcaaaa tacttaaaat
     1441 agagtattaa catttgcgtg aaaccgggtt ataatttggg acaatttaaa ggaggatgta
     1501 catgacgcgc ccgatccttc cggttatagc cctcatactg gcattcgcgt tttacgcccg
     1561 cggggcggaa gccccgatgc cggagggata cggcgtcgtc gtggatgtgg actggccctc
     1621 tcagcccggc cacaggagat tcaacgtcgg aaacggcgac gtcgtcacgg ggagatacgg
     1681 ctggtacagc ccgcccggag ccataggccc cgccgactat aacgtgctca taatcacgaa
     1741 accgataagc aaggcgcgca catatatcgt gttcgaggcc acgaacatag accccgaggt
     1801 gctgaagaag aagctcggca gactcgaagg cgagattcag gacgtatgtg acctcgaagg
     1861 cgcgctcaag ccgggcgata tggtgcacat aaccaccgag gacatccacg gcctcacaaa
     1921 agtcgagcta cattttacac gcggagaaga ccagaagcac gtggcgaggg tttcgttcat
     1981 aaaccacctt gtacatccga tcccggaaac ggaccccgca tatccgtaca tcccctacgt
     2041 gtccgagatc gagtaaccgt aatacttacc tagagtgaaa atacgaagcc cgtatcccca
     2101 agatacgggc ttatcatata aagcgctccc tgttccaatc ccggttaact tttcatcatc
     2161 tcattttagg caccgcgggt cccaggttct gtcgaacgcg ggatgggacc cccgcgatgc
     2221 tttgtacaat tctgaacgac ccgccgctca aggtcagccg tcaaaacatc ttttgctgct
     2281 caaggacgtt ttcctcgggg agattcctgg ccgtgtaaac tatctctttc acgttcggcg
     2341 gaacgccgcc cagggtatag gaaacgagca tgccgttatc aatctgcgcg gtaatttcgt
     2401 aacccgtcgt gagcatcttt tcctgagcct tgtctatccg aatctgatgc tcaataccgg
     2461 tcgcagtctc cttaacgttc aactggtttt tgcccacgag agtgataact ccctgtatga
     2521 tctgggaacc ctcggcaggc atagccgagt cgccctgcaa tgcgggctgc attccttccg
     2581 tagctggctg ctcgtattgc atttcggccg gttggtcggc cgcgctgctg cccataacac
     2641 ctaaccccac gaaagcgaaa agcataacgg taacaaacat ggtcattaac tttttcattt
     2701 gcttcacctc ctgtatttgg attaaagtaa tgaccttctt aagggctgga attgcaaatc
     2761 gtatgccata tgatcggcat tatccgcaat agaaaatgta tgtaaggaca gacggttggg
     2821 tgaattcaaa ttcagttcgg aaatgcagcg tatgtcaatt gtacatatac gaacggttga
     2881 ttgtgtatgt aatgtaacag aacaaaacgg cagccttttt gaagagttaa ttcgacatca
     2941 tttacataca agaaaagccc gtatcttcgg ccaagaagat acgggcttta cgtaaaaagc
     3001 gctccctgtt ccaattccgg ttaacttttc atcggctcat ttttttaggc accgcgggtc
     3061 cctggttcgg acgaacgcgg gacgggaccc cgcgatgctc tacacagttg tcgatgacgc
     3121 gccgctgggg ggccgccatc aaaaggcttt ttgctgttca agtatgttgt cctggggaag
     3181 atcctcggcg gagtaaacta tcttctccac gtcgggcggc actccctgct ctacgaagtt
     3241 gacgagccta ccgttattga gctccgcttc tatattaaaa ccggtcgtaa gctgcttctg
     3301 ctgggcttcg gttatcatga tctgatgttc aatgccggta ccgctctcga ccatcctcat
     3361 ttcgttgtcc ccgaccaggg ttacagtccc tataacagtc cccgatgagc cttccatgaa
     3421 ttgggggata tactgacgcc gctccatctc ggtcgggtcg tttgcggcgc gggtcctctc
     3481 aacctgtgtc tcgaattccc tcaccatgat gtcggtatta gggctcccat aaacgtcctg
     3541 ctcctgaacg gccgggcttt cgccctcgta ggagtacgct ccgagcgagc ttcccgcgat
     3601 gagtaatccg cagattgtat atgtcagaat ccttttcatt gtcacacacc tcctagtgcg
     3661 cgtttgtgtt tctgttacgt atatgatctg caacgggtgt gccagccggg ggtatgaact
     3721 aatgttgtgt cattcagcat ggttgcggat gcaggtatgt atcgggcgca ctgcatcggg
     3781 gacttactgt atcgatagga actgtcccgg ctgtatgaaa cgggacagcg ggcgcgggct
     3841 taacacacga ggcatcgggg ggggtatgca tatacaaccc tatcgccgga tcaggccggg
     3901 aacgctccct gaatcgaaag ccggtctgcc atcctctccc ggccggttat tgacatcggg
     3961 actagtcctg ttattatttt gataacgaag ttcattatca cattcaccct tggggaggag
     4021 ggaatgaatg aaggggacaa tcaattacat caaatcaggc ataaaaacta atattttcct
     4081 gtccttcgcg ctttctcttg tattcctttc gtgcggcgga tcgggaaatg aaaacagggt
     4141 ggacacgaac gccgtgctca gcgatctggc ctacaaggta attctcgacg actatcagca
     4201 gctgcttgac aggggacagg acctggaaag cgctatcggg acgctctccg gggacataac
     4261 ccccgggaac gtcgagaagg cgcgcgatgc gtggaaggcg gcgagggagc cctgggaatc
     4321 gagcgaaggt ttcctctacg gccccgtgga tacggagggc atagacccgt cgatagacag
     4381 ctggcccgtc gatacctccg gcatacaggc gattatcggg agcccggacg atatagacac
     4441 acagtatgcg gcccagttag acggcaccct caagggatat cacacgatgg agtacctgct
     4501 cttcacgatc gggggtgatt tcgtcacggc cgaagagaca gccgattacc tcgcgtccga
     4561 cccgaggctc gtcgagtacc tccacgcgat aatagtcgat ttcaacgcgc aggtagggaa
     4621 gctcatcgta ttctggtctc cgggcgagag cgattacgcg ggcgagctcg cgaccgccgg
     4681 ccaggggagc aacgtttacc ccacgctcaa ggccgggctc gaagaggtcg taaacggcat
     4741 actcggcatc gcccacgaag tggggaacga gaagctgaac gtgcctttct cggagcaggc
     4801 cccggtgttc gtcgaatcga ggttcagctc gaactcgatc acggatttta ctaacaacat
     4861 actgagcata gacaagatat atttcggccg gaacggaagc ctttcactat cagacatagt
     4921 aggcggcata gatgcgcccg tgaacgacga cgtgcgccag gcgatagacg aggccgtagc
     4981 ggcgataaac gacataccgg agccttttaa cgagtcggtg ctcgaccccg ctctcgcgcc
     5041 cgttatccag aacgcgatcg acagggtagt cgagctgagg ctggtgctcg agaacgaggt
     5101 gtatccgatc atgttccccg gaggcaacaa acccccggtg gacgaaacgt agaagtggct
     5161 cgcggtttcg gataaacagc aggaaagggt gagaaaaatg agaagggaga taatacgcgg
     5221 gattctggtg tccgttatga gcgtaattat attgacgggc tgcgacggcg gagaaagtga
     5281 cggcgggggc ggcataggag gcacggacga gctgatgctc ggcggggaca cgacggtgtt
     5341 cgacgcatcg agccaggcgt tcaatttccc ggcaacgggg ataacggaag aaggactaat
     5401 aaaacacgat caaggggacg gggcgttcga cgcaattttc gttagctcac cctcccccgt
     5461 caatccgggt ttgggacccg tattcaataa caactcttgt caaagctgtc acgccaacaa
     5521 cggccgtggc aggcccgacg ataccctgac ctccctactc ttcagggtaa gcgtcccggg
     5581 cgaatccgcg gtcggcggtc ccaacccggt ggcggggttc ggcctccagc tccagacgcg
     5641 ctcgaccgtc ggcaccggga aggaagcggg ggtaacgatc gaatacaccg agacccccgg
     5701 agaatacggg gacggtacgg aatactcttt aagggctcct gtttacacca ttgtcgatcc
     5761 gtatataccg attccggtgg agatactgat atccccgcgc atcgcgcccc cggtcttcgg
     5821 gatcgggctc ctcgaggcga taccagagga cgagattctc gctaacgcag acccggcgga
     5881 cgaggacggt gacggtatct cgggcaggcc caattatgtg tgggacttcg agaaagggac
     5941 tcaggcatta ggcaggttcg gatggaaggc gaacacgcca acagcgatac agcaggcggc
     6001 agacgcgtat aaccaggaca tgggagtaac ctctcccctc ttcccgacgg aatcgagcgc
     6061 ggggcagacg caggaggacg gcctgtccga cgacccggag ataaatgcag atacgctcga
     6121 cgatactgtt ttctatatac a
//
LOCUS       DASSJH010000193         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_696813, whole genome shotgun sequence.
ACCESSION   DASSJH010000193 DASSJH010000000
VERSION     DASSJH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696813"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFJ67_04140"
     CDS             <1..761
                     /locus_tag="VFJ67_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289607.1"
                     /translation="KTTSGRNIAMFDYDFRKGRVVSPPATFLRYTGTGFQTLAGMAVG
                     PDGLYIAPILPLSDGRSAVLKVTYDPQHSHPYTLESETTATVLLESKGCFGCHMYGES
                     GWGTAGPNLDSEKLVARVSERLDSAAYRARVKELDELETEPYKSYREERAEVLRKNGI
                     GRVRAWTKYHIMEPKFDNPESAMPNLGVNDKEAEIITKFLVPDPPDTRTEVYQDFSIV
                     IPELRYRYLVYAFLLGSAVTLLIVGGYTAARRKK"
     gene            912..2840
                     /locus_tag="VFJ67_04145"
     CDS             912..2840
                     /locus_tag="VFJ67_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7289608.1"
                     /translation="MRMRFWPVIVIFGWLVFYPVSSSGQEENRTLPATRTLEPVVVTG
                     SAYPLELSKSTGSVTVITEEDIKNSHAVSVGEVLEQVPGLYVDQPASRGGVTSVYIRG
                     GDPNFTLVLIDGVKVNDPTNSRGGSFDFSSLSTDDIERIEIVRDPMSSLYGSDALSGV
                     INIITKKGKGKPKASAEAMAGRYGQYQLLGGVSGGGPFYDFSVTGSWLDDGEQVEGSS
                     FKSPSVTANLGLLKRDDMELSTTFRYSHIDSTSFPDDSGGPEFAVFRGTDDRNIDQYL
                     LGLGYTHSPAGWWEYGIKLSFYNNKEKFSSPGVAPGERDPFGIPPNEADTDYKSYDAN
                     ISSTFSPIDGLDIVAGFESEFQDGSSEGMILGDFPLPASFDLSRYILSPYLEVQFGMI
                     ENLFVVVGAREDFPEGFDPAFSPRAGLSYKIARTGTILRGSWGEGFKLPSFFSLANPI
                     VGNPDLVPEESRSLDLGITQGVWDERLSAQFNVYYNEFKNLVDFEEGPPPILVNRSRV
                     TVKGFELIGNIRPWEEFQLSGSASYADSDIKGTDEPLRNRPKWWGELTLLYRPSRYVK
                     FNLDAVFTGSVPDSSIPTGDVTLDPYDVYNVALTWTPRDRISVYVAVDNLLNEQYEQY
                     VGFPAPGVSPRAGVRITF"
BASE COUNT          728 a          910 c          936 g          624 t
ORIGIN      
        1 ccaagacgac gagcgggaga aacatagcca tgttcgacta tgatttccgg aagggcaggg
       61 ttgtctctcc gccggcgacg ttcctcagat atacgggtac cggattccag accctggcgg
      121 gtatggctgt aggccccgac ggactttata tcgctcccat actgccgtta tcggacggca
      181 ggagcgctgt gctaaaggtc acttacgacc cccagcacag ccacccctac acactcgaaa
      241 gcgagacgac cgcgaccgta ctccttgaat cgaagggctg cttcggctgc cacatgtatg
      301 gagagagcgg ctggggcact gcggggccca atctcgacag cgagaagctc gtcgcgaggg
      361 tcagcgagag actcgactcg gcggcataca gggcccgcgt gaaggagctc gacgagctcg
      421 aaaccgagcc gtataaaagc tacagggagg aaagagccga ggtgctacgg aaaaacggga
      481 tcgggagggt gagggcatgg actaaatacc acataatgga gcctaaattc gataatccgg
      541 aatctgcgat gccgaatctc ggggttaacg acaaagaagc cgagatcatt accaagttcc
      601 tcgtccccga tccgcctgat acgaggaccg aggtatacca ggatttcagc atagtgatcc
      661 cggagctcag atacagatat ctggtctatg cgtttcttct cgggtccgct gtaactctcc
      721 tgatcgtcgg cgggtacacc gccgcgcgca gaaaaaaata atagttcggg atggcttgta
      781 tttgccgggt ccgccggaca cctgcgtgcg tacacttatc ccgcaccttt actcggggcg
      841 ggtttcgaaa tatcatcatc cgggcgtggt ataaattgcc tgtaaacggg agacggcgcg
      901 gacggcatct gatgagaatg agattctggc ctgtaatcgt gatattcgga tggctggttt
      961 tttatccggt ctcgtcttcg ggacaggaag aaaaccggac tcttccggct acccggaccc
     1021 tggagcccgt tgtcgttacc gggtcggcat accctttgga gctcagcaag agcacgggaa
     1081 gcgtaactgt cattacggaa gaggacataa agaacagcca cgctgtcagc gtgggcgagg
     1141 tcctggagca ggtcccggga ctttacgtcg atcagcctgc ctcgcgcgga ggcgtcacgt
     1201 cggtctatat ccggggaggc gatcccaact tcacgctcgt gctcatcgac ggcgtgaagg
     1261 tgaacgaccc gaccaacagc agggggggct cattcgattt ctcctcgttg agcacggacg
     1321 acatagagcg aatcgagatc gtacgcgatc cgatgtcgtc cctctatggc tcggatgcgc
     1381 tgagcggcgt cataaacatc atcacgaaga aagggaaggg aaagccgaag gcgtctgccg
     1441 aagccatggc cgggagatat ggccagtatc agctcctggg cggcgtctcg ggggggggac
     1501 ctttctacga cttctccgtc acgggctcat ggctcgacga cggggagcag gtggaaggga
     1561 gctccttcaa gagtccgagc gtaaccgcga acctggggct cctcaagagg gacgacatgg
     1621 agctgtcaac cacattcagg tactcgcaca tcgacagcac gagcttcccg gacgacagcg
     1681 gcggcccgga attcgcggtg ttccgcggta ccgacgacag gaacatcgat cagtaccttc
     1741 tgggactcgg ctacacacac agccccgccg gctggtggga atatggaatc aagctctctt
     1801 tctataacaa taaggagaaa ttctcctcgc ccggggtcgc ccctggcgaa cgcgatccat
     1861 tcggcatccc ccccaacgaa gcggatacgg actacaagag ctacgacgcg aatatctcga
     1921 gcacgttttc ccccatcgat ggtctcgaca tagtcgcggg gttcgagtcg gagttccagg
     1981 acggatcgag tgaagggatg atactcgggg atttccccct gccggcttcc ttcgacctca
     2041 gcaggtatat cctgtccccg tacctggagg tccagttcgg catgatagag aacctgttcg
     2101 tagtggtagg cgcgagggag gacttccccg agggtttcga tccggctttc agcccgagag
     2161 ccggattgtc atacaagatc gcccggaccg gcacgatact caggggcagc tggggcgagg
     2221 ggttcaagct cccgtccttc ttttccctgg cgaatccgat agtcgggaac ccggacctcg
     2281 tgcccgagga gagcaggagc ctcgacctcg gcatcactca gggcgtctgg gacgaaagac
     2341 tgagcgcgca gttcaacgtg tattacaacg agttcaagaa tctcgtggac ttcgaggaag
     2401 ggcctcctcc catactcgta aaccgctccc gcgtgacggt gaaggggttt gagcttatag
     2461 gaaatatacg accgtgggag gagtttcagc tgagcgggag cgcgagctac gccgacagcg
     2521 atataaaggg tacggacgag ccgctccgga acaggcccaa atggtggggg gagctcaccc
     2581 tcctctacag gccgtccagg tacgtgaagt tcaacctgga cgccgtattc acggggagcg
     2641 tccctgattc ctcgattccg accggcgacg ttacgctcga tccgtacgac gtgtataacg
     2701 tcgccctcac gtggacaccc agagaccgga taagcgtgta cgtagcagtc gataacctcc
     2761 tcaacgagca gtacgaacag tatgtagggt tcccggctcc gggcgttagc ccgagagcgg
     2821 gcgtgagaat cactttctaa cgcgcagaat ctgcgctcat atatgattat tatccactcc
     2881 tctgcacgac caacaaccaa atttaaaccc gttcgtggcg aagccaaaaa cttgcggcac
     2941 agaatgtgag ctcatgtacg tttcttccat acctccacag cttaggccgg cggcgcagcc
     3001 ttaccgtcct cacggaacca gcggcacgca atgtcttgca cggaacctat gctcccccac
     3061 atagcgtgtt tctacgatcc tcccgccact ccactgccta tacaagaatt tccctctccc
     3121 ttcagggaac gaatgcgaac aagtgagcat tcggttgcct tggatcagag acacagaagc
     3181 acattgaccg ccgtgtgt
//
LOCUS       DASSJH010000194         5845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1560697, whole genome shotgun sequence.
ACCESSION   DASSJH010000194 DASSJH010000000
VERSION     DASSJH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560697"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..3718
                     /gene="smc"
                     /locus_tag="VFJ67_04150"
     CDS             209..3718
                     /gene="smc"
                     /locus_tag="VFJ67_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET7289609.1"
                     /translation="MKIKSLEITGFKSFYEKTRINFDIRLSAIVGPNGCGKSNVLDAI
                     RWILGEQNPRQLRANVMEEIISNGSEFLRPLGMAEVSLVMEKVPNYNFEEVEIKRRVF
                     RSGETEYYLNGVPCRLKDITDIFIDTGSGARAYSVIGQGRVDQMITAKPEDKRTLIEE
                     VAGIRKYKIRRRETETRIKTTRENLSRVKDMANEVKRQMDTLSLQAKHAGEFRELSEE
                     ARRLEAGILRARLERIEKKKNSVTGEKTAVEQAIAKSEEETIQVINLLKKLNNQSSIT
                     DDNIRQLENEVFRTKTDLQSKISSQELIKSEISSIERFIEKIESESTLLSGERENLSA
                     QTGLKQGACEEVRHELEKEKTEIEEKENQLAEIKAGFAAIRAEHQEIRASLFKTLDEY
                     SSLKGAALGYEKELRDLYTKKEIIEKEYSEIEQERKTAEEEVSRLGRILAENEARKQN
                     IAEAKGRMETSLSSLRETQAVKRGEYDGLLERLNESTSRLTVLNQVQANYEWLPEGIR
                     NFILEHKGNGVLGTVSDFITVPEGYERAVEAVLGDRIKWILVEGNAQALSAVDSLRQR
                     SLGRGTFVSAGKDRVIAESNGRPANSKPLSEIVSLDDRLSTIGELLDSVYMVPTLNEG
                     LRMQAETGGGASFVTAEGDYLHPDGAISGGTAQPGVLERKREIENLKTQIGALESGVE
                     RLSGEIEAAELEIKNLNDVIRGYESELVETEIREAETRKDILNHRNNLEKLEKRIYTI
                     SQNIRSTDVETGEKLRLMDETRTKITVLDSEKAVLEQRFGEVEVRIQKAEEEERSVER
                     EIAEKKVRCASFVEKEKSLIEDLTELGKRITDIDARLDNESRNIEEKKREKLGFIEKD
                     RYTVEEISLISNTINEHEEKLRSMKNERGAVAEEIRVTSEKRESLNAGLSDLRLRNNS
                     LELDLNGLQIENENIRDIIERSNLSDIIRAQAEAGDYAEINIQQEEGKLKKLQAQIEK
                     FGPVNLLAPEEYNKLEERYKFLDEQMEDLNQAINSLGKAISKIDKESEKRFRETFELM
                     DQKFQQIFNRLFRGGEGKLVLTEPDSMLESGVEVMVRPRGKKFQSITLLSGGEKALSA
                     IALIIAACLIKPAPFLLFDEIDAPLDDRNTSYFIELVKEIDNNSQVILITHNKKTMQQ
                     VNSLIGITSNRSGTSTVVSVNLN"
     gene            3866..>5845
                     /gene="secA"
                     /locus_tag="VFJ67_04155"
     CDS             3866..>5845
                     /gene="secA"
                     /locus_tag="VFJ67_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007545685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET7289610.1"
                     /translation="MISYIMKKIVGSQNDREIKRLSALVEMVNDRESEFQSLPNAEFA
                     PRTVELKERIMNGFNGNGDLTEDAHFAKLEEGLIEILPEAFGLVREASRRTLGMRPFD
                     VQLIGGLVLHLGRIAEMKTGEGKTLVAALPLYLNGITGLGAHLVTVNDYLARRDAMWM
                     GPIYKLLGLNIGVINHEISYLVEWEDPARAENAIKNNLSVWPAEYSDMEIPPEKNLDV
                     LASFKTRLVECSRKEAYRAHATYGTNNEFGFDYLRDNMKFSLNDYVQREHSYAIVDEV
                     DSILIDEARTPLIISGPSEESTQLYYDINNVVAKLKPEVDFTLDEKTRQVTPTEEGSN
                     KVERALGISNVYDPANLEILHHVIQGLRAHNLFHRDVDYMVKDGKVIIVDEFTGRLMP
                     GRRWSDGLHQAIEAKEDVEIENENQTLATITIQNYFRMYRKLAGMTGTADTEAFEFKN
                     IYDLEVNVVPTNKPMIRADRNDSVYKSEREKFGAVAEEIKELHEKGRPALVGTTSIEK
                     SERLSGMLQKLRIPHQVLNAKQHENEAEIVAQAGRIGAVTIATNMAGRGTDIILGGNN
                     EYLARDILRKKFRIDPQEALPEQFEEAMLEAKSVCEDEKKRVLDLGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFFISLEDDLMRIFAS"
BASE COUNT         1712 a         1421 c         1720 g          992 t
ORIGIN      
        1 gactacatcg aggcaaagag agaggtcaga cttatcaata cccgcaaaac gaacattaag
       61 agcgacaaaa agatagaaaa aaacttctgg tccagcttat ttaaaaagta atttaatcca
      121 gatgccccga aatcccgcaa gccgtattat ttcttcggat atttactttt tttaaacttt
      181 tgattatctt cactcattag attaggcgat gaaaatcaaa tccctggaaa ttaccggatt
      241 caagtccttt tacgaaaaga cccgcataaa tttcgacata cgcctgagcg cgatcgtagg
      301 ccctaacggc tgcgggaaat cgaacgtgct cgacgccatc aggtggatac tcggcgagca
      361 gaacccgagg cagctgaggg cgaacgtaat ggaagagatt atctcgaacg gcagtgagtt
      421 cctgaggccg ctcggaatgg cagaggtctc cctcgtaatg gagaaggtgc ctaactacaa
      481 cttcgaggaa gtggagataa agcgccgggt tttccgctcc ggggagaccg agtattactt
      541 aaacggcgtc ccgtgcaggc ttaaggacat cacggatata ttcatcgata cgggctcggg
      601 agcgagggct tactccgtca taggacaggg aagagtggac cagatgataa cggccaagcc
      661 cgaggataaa aggacgctca tagaagaagt ggccggcatc aggaaataca agataaggcg
      721 cagggagacc gagaccagga tcaagaccac gagggagaac ctgagccgcg taaaggacat
      781 ggcgaacgaa gtaaagaggc agatggatac gctcagcctc caggcaaagc acgcggggga
      841 gttcagggag ctttccgagg aggcgaggag gctcgaagcc gggatcctca gggccaggct
      901 cgagaggatc gagaaaaaga agaacagcgt gaccggggaa aaaacggccg tcgagcaggc
      961 gattgccaaa tcggaagaag aaacgataca ggttattaac ctgctcaaaa aattaaataa
     1021 ccagtcctca attacggacg acaacataag gcagctcgag aacgaggtct tcaggaccaa
     1081 aaccgacctc cagtcgaaaa tatcctcgca ggagctgata aaatcagaaa tctccagcat
     1141 cgagaggttc atcgagaaga tcgagagcga gtccacgctg ctcagcggcg agcgtgagaa
     1201 cctctcggcc cagaccgggt tgaagcaggg cgcgtgtgaa gaggtcagac acgagctcga
     1261 aaaagagaag accgagatag aggaaaaaga aaaccagctt gcggagatca aggccgggtt
     1321 tgccgctata cgcgccgagc accaggagat aagggcatcg ctgttcaaga cgctcgacga
     1381 atacagctct ctaaaagggg cggcgctcgg ctacgagaaa gagctcaggg acctctatac
     1441 gaagaaagaa atcattgaaa aggaatattc ggaaatagag caggaaagaa aaaccgccga
     1501 agaagaggtc tcgaggctcg ggcgcatact cgccgagaat gaagccagaa agcagaatat
     1561 tgcggaagcg aagggcagaa tggagacctc tctctcgtcc ctccgtgaga cacaggccgt
     1621 aaaacgcggc gagtacgacg gactcctcga acgccttaac gagagcacct ccagacttac
     1681 tgtgctcaac caggtccagg cgaactacga atggctcccc gaggggataa ggaatttcat
     1741 tctcgagcat aagggaaacg gcgttctcgg cacggtttcc gatttcatta cggtccctga
     1801 aggatacgaa agggcagttg aagcggtgct cggggacagg attaagtgga tactggtaga
     1861 ggggaacgct caggccctga gcgcggtcga ttccctgaga cagcgctccc tcgggagagg
     1921 gacatttgtc tccgcgggga aagaccgggt catcgcggaa tcgaacggga ggcccgcgaa
     1981 ttcgaagcct ttatccgaga tagtcagcct ggatgacagg ctgagcacga tcggggagct
     2041 actggatagc gtatacatgg tacccacgct taacgaaggt ctcaggatgc aggcggaaac
     2101 gggaggggga gcgtccttcg tcaccgctga aggcgattac ctgcacccag acggggcgat
     2161 ctccggagga acggcccagc cgggggtgct cgagagaaag cgggagatcg agaacctcaa
     2221 aacccagatc ggcgcacttg aaagcggcgt cgagaggtta agcggggaaa ttgaagctgc
     2281 ggagcttgag ataaagaacc tgaacgacgt gatcaggggg tacgagagcg agctcgtcga
     2341 gaccgagatc agagaggctg agaccaggaa ggacatactc aaccacagga acaacctcga
     2401 aaagctggag aaacgtatct atacgatcag ccagaacatc aggagcacgg acgtcgaaac
     2461 cggcgagaag ttgaggctca tggacgaaac gaggacgaag ataaccgttc tcgattccga
     2521 gaaagccgtc ctcgagcagc ggttcggcga agtcgaggtc aggatccaga aggccgagga
     2581 ggaggagcgc tctgtcgaga gggaaatcgc cgaaaaaaag gtcaggtgcg cctcgttcgt
     2641 agaaaaggag aagagcctaa tcgaggattt gacggagctc ggaaagagga taacggacat
     2701 cgatgcgcgt ctcgataacg agtcgaggaa catcgaggaa aagaagcggg aaaaactcgg
     2761 ctttatagag aaggacaggt atacggtcga agagataagc cttataagta ataccattaa
     2821 tgagcatgaa gagaaacttc ggtcgatgaa gaacgaaagg ggcgccgtag ccgaagagat
     2881 ccgggtcacg agtgagaaga gggaaagcct gaacgccgga ttgtcggacc tgagactccg
     2941 gaacaattcc ctcgagctcg acctgaacgg ccttcaaatc gagaatgaaa acatcaggga
     3001 catcatagaa aggagtaacc tgtcggatat aatccgggcc caggccgaag ccggggatta
     3061 cgccgagata aacattcagc aggaagaggg gaagctgaag aagcttcagg cgcagatcga
     3121 gaaattcggg cccgtcaacc tgctcgcccc cgaagagtac aacaaactcg aggagagata
     3181 taaattcctc gacgagcaga tggaagacct taaccaggcg attaattcgc ttggaaaagc
     3241 tataagcaag attgacaagg agtccgagaa gaggttcaga gagacgttcg agctgatgga
     3301 ccagaaattc cagcagattt tcaaccgcct gttcaggggc ggggaaggca agctggtgct
     3361 gaccgagccc gacagcatgt tagaatcggg cgtggaggta atggtgaggc cgaggggtaa
     3421 gaaattccag tccataacac tcctttcggg cggcgaaaag gccctctctg ccatagccct
     3481 gatcatcgcc gcctgcctga taaaacccgc gccgttcctt ctgttcgacg agatagacgc
     3541 cccgctcgac gacaggaata cttcttattt catagagctg gttaaggaaa tcgacaataa
     3601 ttcccaagtg atactgataa ctcacaacaa gaagaccatg cagcaagtca actccctgat
     3661 cggcataact tccaacaggt ccggcacttc gaccgtcgtt tcggtcaacc tgaactgata
     3721 cctgcgcaag aggcggaact atatataaaa caggggatta aatcttaatt gcggctgact
     3781 cccgtataaa taagcaaaat ttgtgatgcc cgggttttac ccttatactt ttaccggtcc
     3841 caatgtttcg gaggattttt agaaaatgat ttcttatata atgaagaaga tcgtaggttc
     3901 ccagaacgac agggaaatca agagactgag cgctcttgtc gaaatggtga acgaccgtga
     3961 aagtgaattc cagtccctgc ccaacgccga gttcgcaccg cgcaccgtgg agttaaagga
     4021 gaggataatg aacggcttca acgggaacgg cgacctcacc gaagacgcgc acttcgcgaa
     4081 gctcgaggaa gggctgatag agatactgcc cgaggcgttc ggtctcgtga gggaggcttc
     4141 acggaggaca ctggggatgc gtcccttcga cgtacagctc ataggggggc tcgtgcttca
     4201 cctgggcagg atcgctgaaa tgaagacggg agaaggaaaa acgctcgtcg ccgcgttgcc
     4261 gctctatctg aacggaataa cgggtcttgg agcgcacctc gtgacggtga acgactacct
     4321 ggcgaggcgc gacgcgatgt ggatgggccc catatacaag ctcctcgggt tgaacatcgg
     4381 ggtcattaac cacgagatat cctatctcgt cgaatgggaa gacccggcaa gggccgagaa
     4441 cgcgataaaa aacaacctca gcgtatggcc cgccgagtac tcggacatgg aaataccgcc
     4501 cgagaagaac cttgacgtgc ttgcctcatt caagaccagg ctcgtagagt gctcgagaaa
     4561 ggaggcttac agggcgcacg ctacctacgg gacgaacaac gagttcgggt tcgattacct
     4621 gagggacaac atgaagttct ccctcaacga ttacgtccag agagagcaca gttacgcgat
     4681 cgtcgacgag gtggacagca tcctgatcga cgaggcgagg accccgctca tcatatcggg
     4741 cccgtccgag gaatcgacgc agctttacta cgacattaac aacgtggtcg cgaagcttaa
     4801 accggaggtc gatttcacgc tcgacgaaaa gacgcgtcag gtcacgccaa ccgaagaggg
     4861 atcgaacaag gtcgaaaggg ctttgggtat aagcaacgtc tatgatcccg ccaacctcga
     4921 gatcctccat cacgtcatac agggcctccg cgctcacaac ctcttccaca gggacgtcga
     4981 ctacatggtc aaggacggca aggtaatcat cgtcgacgag tttaccggga gactcatgcc
     5041 gggaaggcgc tggagcgacg gtctccatca ggcgatcgag gccaaagagg acgtcgagat
     5101 cgaaaacgaa aaccagacgc tggccacgat aacgatccag aactacttca ggatgtacag
     5161 gaagctcgcc gggatgacgg gtaccgccga cacggaagct ttcgaattca agaacatata
     5221 cgacctcgag gtgaacgtcg tacccaccaa taagcccatg ataagggccg acaggaacga
     5281 ctccgtttac aagtcggaga gagagaaatt tggcgccgtc gcggaggaga taaaagagct
     5341 ccacgaaaaa gggaggccgg cactcgtggg aactacgtcc atcgagaaat cagagaggct
     5401 ctcgggcatg ctccagaagc tgcgcatacc ccatcaggtg ctcaacgcga agcagcacga
     5461 gaacgaggcc gagatcgtag cgcaggccgg aaggatcgga gcggttacca ttgcgacgaa
     5521 catggcgggc aggggcacgg acatcatact cggcggtaat aacgaatacc tcgcgaggga
     5581 tatactcaga aaaaaattca ggatcgatcc gcaggaagcc ctcccggaac agttcgagga
     5641 agccatgctc gaagcgaagt cagtttgcga ggacgagaag aagcgggtgc tcgatctggg
     5701 aggactccac ataataggca cggagcgtca cgagtcccgc aggatcgaca accagctgag
     5761 gggaaggtcg ggcagacagg gagacccggg ctcctccagg tttttcatat ccctcgagga
     5821 cgacctcatg aggatattcg cctcg
//
LOCUS       DASSJH010000195         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_585548, whole genome shotgun sequence.
ACCESSION   DASSJH010000195 DASSJH010000000
VERSION     DASSJH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585548"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFJ67_04160"
     CDS             <1..596
                     /locus_tag="VFJ67_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7289611.1"
                     /translation="YKIPPYDETIEYVNRVYAHYDYLKKHPVDRNIDSMIASRKDGVK
                     SVVTSKKSFVYVERLGGAETGVSQAALSDTGKKPVLTVKAEKATVNTTNATAKNTTVK
                     KSIAAPVSRPVEKARQGQYTVQLASFPDLRIAREMEESLKSKTYPAYIETVELPGKGT
                     WYRVKVGGFTTEDEAKKYGNDMITAEPDVKSIIVTLN"
     gene            923..2920
                     /locus_tag="VFJ67_04165"
     CDS             923..2920
                     /locus_tag="VFJ67_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET7289612.1"
                     /translation="MKSIIMAILLIAAFGFFFYNLYNLYKLLRLGKFENRFDRIGERI
                     RLVLVYVFGQKRLVKNYKFAGIEHFMIFWGFVIVTVGTIEILIGGVIPGFRLIPGSAH
                     NVYEFILDIVQTLVMIAIIMGIINRTTIAKRREVNGLDAVVILGLIFGLMVTALGAMA
                     TNVAMGEAEAKWVPVSSAISSVFFSGMSTSGLEYSHEFFWWFHVLIVLGFLNYLPYSK
                     HSHVFTAIPNVFFQNLKPRGALKPIDFENVPDDVDHFGAGKIQDFTWKDILDAYTCTE
                     CGRCTDNCPAWHTEKPLSPREIVVKLRHYASSEGKALLNGGSSNGSEASTIPGPEWVT
                     EDELWACTSCNACVEQCPLFIDQMGKIMEMRRFLTLEGRLTGTAVKTLQKLQTQGNPW
                     GFSEGDRAKWISGYEGVNVIGENGVEDASAFDYIYWMGCYGGYDPRGQKIAQSVVSLL
                     TQAGVSFAVLGPMERCTGDPARRLGEEALYQMLATQNIETLNGIKVKKVIANCPHCFN
                     TIKNEYPQFGGNYEVINHTQLLNELVKQGRLKPTKEINQGVAYHDPCYLGRYNRVFDE
                     PRELLQSIPGLRLIELDRKRERSFCCGAGGGKIWMEEDAPRVNWNRFEEVESKNPDTL
                     AVACYFCDTMFEDASKHAGNDKMEIKDISVILRESVEADKR"
BASE COUNT          809 a          736 c          871 g          686 t
ORIGIN      
        1 gctataagat tcccccttac gatgaaacga tcgagtatgt gaacagggtc tacgcccatt
       61 acgattatct gaagaagcat cccgtggaca ggaacatcga ctccatgatc gcgagcagga
      121 aagacggggt taagtccgtt gttacgtcca agaagtcgtt cgtatatgtg gaaagactgg
      181 gcggagccga aacgggcgta agtcaggccg ctctttcgga taccgggaaa aaaccggttt
      241 tgacagtgaa agctgaaaag gcaacagtta atactactaa tgcgactgcg aaaaatacga
      301 ctgtgaagaa gagcattgcc gcgcctgtga gccggcctgt cgagaaagcc cgtcaggggc
      361 aatacaccgt tcagctggcg tcattcccgg acctgcgcat agccagggag atggaagaga
      421 gcctcaaatc caagacgtac ccggcgtaca tcgagacggt cgagctgccc ggcaagggga
      481 cctggtacag ggtcaaggtg ggcgggttca cgacggaaga cgaggccaag aagtacggga
      541 acgatatgat aaccgcggag ccggacgtca aatccatcat cgtaaccctg aactgacgat
      601 cccttacccc atgacatcaa acggctcctg acaatgtgac gggctttgtc ccggtctgtc
      661 ttacttttgc ggctcgttac acggcttttc cggttcgtga ctcctctatg acgtgacggt
      721 cttcgatata tattatatag cgtatatata tatcggccgg gctttgccgg aatgcgcgaa
      781 accggtcaat ttatctcatt attgaaatta attacgccat ctgaccgaaa agccttaatt
      841 ccttgactat cacagtgctt ttttttaaga tacagagaga aggtctggca gttcgggcgc
      901 ccgcacgtta aggagacaat ctatgaagtc gatcataatg gcaattctgt taatagccgc
      961 tttcgggttt ttcttctata acctgtacaa cctctacaag ctgctccgtt tgggcaaatt
     1021 cgaaaacagg tttgacagga taggcgaaag gatcaggctc gttctcgttt acgtgttcgg
     1081 ccagaagagg ctggtcaaga attataagtt cgccggtata gagcatttta tgatcttctg
     1141 gggcttcgtg atagtcacgg tcggcaccat agagatacta attggcggcg tgatccccgg
     1201 gttcaggctc attccgggct ccgcgcacaa cgtatacgaa ttcattctgg atatagtgca
     1261 gacgctcgtc atgatagcga taataatggg gatcataaac aggaccacta tagcgaagag
     1321 gcgggaagta aacggtctcg acgctgtcgt gatactggga ctgatcttcg gactcatggt
     1381 cacggcgtta ggcgccatgg cgacaaacgt cgcgatggga gaagccgaag cgaagtgggt
     1441 tccggtatcg agcgctattt cgagcgtttt cttctcgggg atgagcacat cagggctcga
     1501 atactcgcat gaatttttct ggtggttcca cgtgcttata gtcctcggtt tccttaacta
     1561 tctgccttat tcgaaacaca gccacgtctt cacggctatt cccaacgtct tttttcagaa
     1621 cctcaagccg agaggcgcgc tgaagccaat cgatttcgag aacgtgcccg atgacgtaga
     1681 tcacttcggg gcgggcaaga tacaggattt cacatggaaa gacatactcg acgcatatac
     1741 ctgcacggag tgcggaaggt gcaccgataa ctgccccgcg tggcatacgg agaagccgct
     1801 ctcaccgaga gagatagtcg tcaagctcag acattacgca tccagcgagg ggaaagcgct
     1861 attgaatggc ggctcctcga acggcagtga agccagtacg atccccgggc cggaatgggt
     1921 aactgaagac gagctatggg cctgtacgag ctgtaacgcc tgcgtcgagc aatgccctct
     1981 ctttatcgac cagatgggga agatcatgga gatgaggcgc ttcctcacgc tagagggaag
     2041 gctgacgggt acggccgtca agacactcca gaaactccag acccagggga atccgtgggg
     2101 atttagcgag ggcgaccgcg ccaaatggat atcggggtat gagggtgtga acgtgatagg
     2161 tgagaacggc gtcgaggacg cgtccgcttt cgactacatt tactggatgg ggtgttacgg
     2221 cgggtatgac cccagggggc agaagatcgc tcagtccgtg gtgagcctgc taactcaggc
     2281 cggggtcagt ttcgcggtgc tcggtccgat ggaaagatgc acgggagacc cggcgagaag
     2341 gctcggcgaa gaagcgctat accagatgct cgccacgcag aacatcgaga cccttaacgg
     2401 gatcaaggtg aagaaggtaa ttgccaactg cccgcattgt ttcaatacga ttaagaacga
     2461 atacccgcag ttcggcggga attacgaggt cataaaccat acccagctcc tcaacgagct
     2521 cgtgaaacag gggagattaa aaccgacgaa agagatcaac cagggtgtcg catatcacga
     2581 cccatgttac ctcggcagat ataacagggt attcgacgag ccgagggagc tgctgcagtc
     2641 catacccggc ctgaggctca tagagctcga caggaagcgt gaaaggagtt tctgctgcgg
     2701 agccggcggc gggaagattt ggatggagga agacgccccg agggtgaact ggaacaggtt
     2761 tgaggaggtc gaatccaaga accccgatac gctcgccgtc gcatgttact tctgcgatac
     2821 gatgttcgag gacgcgtcta aacatgcggg caacgataaa atggagatca aggacatatc
     2881 cgttattctg agggagtcgg tcgaagccga taagcgctga tcgatggtca agtgctaaaa
     2941 tagatgcata tataatacaa tatagaatta tatatattac attcttacat ctaacagcta
     3001 cctcaactat taccaatttc tttgtatgtt ccatatatga agttaagata gataatcatg
     3061 gacggatttt catgcctgtt tcttgactca ggcggcggct gg
//
LOCUS       DASSJH010000196         7514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1346955, whole genome shotgun sequence.
ACCESSION   DASSJH010000196 DASSJH010000000
VERSION     DASSJH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346955"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="VFJ67_04170"
     CDS             complement(<1..127)
                     /locus_tag="VFJ67_04170"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HET7289613.1"
                     /translation="MSFIKGLTERMGIVGELLQFFIQNKWWWITPMIIILILFAFL"
     gene            complement(194..1981)
                     /locus_tag="VFJ67_04175"
     CDS             complement(194..1981)
                     /locus_tag="VFJ67_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HET7289614.1"
                     /translation="MNILGISCYFHDAAAALLKDGVLIAAAEEERFSRIKHDYEFPEN
                     AIGFCLAAGGIEAGDIDYVMFFEKPFIKFERLLLCSMQTFPRSMKLFREAMVTWLGDK
                     LWIKHLLLKKLGVPASKVLFSEHHLSHAASTFYCSPFDEAAILTVDGVGEWTTAALGV
                     GRGTDIKLIKEIRFPHSLGLLYSAFTAFLGFEVNEGEYKVMGMAPFGRPNYVDKIYEL
                     VHVDDDGGFELDMDYFSFHYSSEKTYKKKFEKLFGDPRDQKAHFFTPSTGYPSYFGEK
                     PADFDELGKRNQFYADIAASIQVVTEQVMLKMANYAYRETGINKLCMAGGVALNSVAN
                     RKILSETPFEEIYIQPAAGDGGGAVGAALYGYHALLGQPRKFVMEHAYWGEEHSASDT
                     EEFLTENNIPYEKIEDDQKLIARVTDALTGGKVIGWHQGRFEWGPRALGNRSILADPR
                     RADMKDIVNVKIKFREPFRPFAPSVLVEKAGDYFALDHPEKHYPARFMLYVTDVHENK
                     RDVLPAITHVDGTGRLQTVRKELNPKYYSLIETFGQATGVPVVLNTSFNLKGEPIVNT
                     PVEAFNSFSKSGMDMLVLGNYIVDKDKLP"
     gene            complement(2025..2300)
                     /locus_tag="VFJ67_04180"
     CDS             complement(2025..2300)
                     /locus_tag="VFJ67_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289615.1"
                     /translation="MSTIQRKKKEEKEKSLLRKIWDGWKKIARKIGDFNARVILTIFY
                     LILLMPFALLVKVFTDPLEIKKNAKKGWQPREENPDITPMEKAVRQF"
     gene            complement(2611..4734)
                     /locus_tag="VFJ67_04185"
     CDS             complement(2611..4734)
                     /locus_tag="VFJ67_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET7289616.1"
                     /translation="MIILDKRKYERIVSELIDAKDIRELVERYSLDRIYFYHILKDIS
                     LGERSPNHKLLKNIAQRRKLSEDFVIEQAKSVLNFLLPYYQSFQTDSLDYYKILNVSR
                     SATDEEIRRNWIELMKAHHPDKAGAEGLDTSKKINEAYEVLSSASKREAYDNKHLPPT
                     PVIVPQHEMKKYYYAGALMVFVFLVVMYASGSGLIFQSPEEKERLVREFEAPDMPNTV
                     YKGDYLDAEKVDKKISEIKPIEQAKRTGEKSAVKEKDATSAAVTQEMPSPAENTVVAD
                     AGPADETAPVEKTDSEDVTETVPAEEFGNAALEPKAEKTIEITEVTPDVSDKETQPTE
                     PVPGEVKPAVKETLAAKSDEAPAPAGKEAGKSPEAPPAAETVKKSAEPSPKQEVLTAE
                     KSGAVKDEQKPGSEIKPESEIKETEKAPDIKLAEKPAAPVKPKGELTEISVPEKENPP
                     SAGEYYTVRQGDSLWTIAHKFGKTTGELVSLNGLSDSKLRIGEKILISGEGVIPPKPV
                     TEVAVKKAEPVPVPVTRPKREITPAPVVAKAQAPVKPPVKTDITSDFNPKLATSAVAV
                     RDTSVRPPEPIPPAAPPDRDSVYGFVTDYVSAYKNKDIDRVKALFMPDAVENGVSISR
                     VLSSYSSNFSNLDIMKYDVKVSRISVENSLGYVRGEFFITFKDQRTGALKSSKGQINW
                     TLSWTGGSWRIRELTYKIENTDTVG"
     gene            5205..5630
                     /gene="ndk"
                     /locus_tag="VFJ67_04190"
     CDS             5205..5630
                     /gene="ndk"
                     /locus_tag="VFJ67_04190"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7289617.1"
                     /translation="MELERTLSIIKPDGVEKNVIGEVISRFEKNGLRIAALKMVKLTK
                     EDARGFYIVHKERPFYGSLTDFMSRGPVVLMVLEGENAIAKNREIMGATNPAQAAEGT
                     IRKDFATSIEENTVHGSDSPESAAFEIGYFFNALELCGG"
     gene            complement(5815..6513)
                     /gene="lipB"
                     /locus_tag="VFJ67_04195"
     CDS             complement(5815..6513)
                     /gene="lipB"
                     /locus_tag="VFJ67_04195"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET7289618.1"
                     /translation="MDSETKRVIRWEYRGVVSYADGLALQNERRETLRKEGPGAGGYL
                     LLLEHSPVITDGRFGRGGNIMLPPEELEKRGVETYKTGRGGDVTFHGPGQLVAYPIID
                     LRAFGMGARAYVRALEETIIRTLGDYDLKGVTMEKFPGVWAYGGKIASVGVAVRNGIT
                     MHGCAFNVNTDLDYFSMIIPCGLDGVRMTSLREILDEEMDFGAVTRAFVRSFGEVFGA
                     EMREAGEGFSLINE"
     gene            6646..>7514
                     /gene="holA"
                     /locus_tag="VFJ67_04200"
     CDS             6646..>7514
                     /gene="holA"
                     /locus_tag="VFJ67_04200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7289619.1"
                     /translation="MKQKSGGKSSAQDFSSYIASGDPAPVLVFTGGQAFLIEKAVTEL
                     RERLLGPSGDINFITFSGESASGREIADNASTYPMFSKKKLVVVKNADKLSAKELTAL
                     EPYISSPSPSACLVLVFGDGKKPKLGGGKHVAAFDFSMEKGSAPSAVREEARKLGYDI
                     TRAGAEALIGLVGEDLQEIHNELVKISLYKGDKKTIGPEDVEALTKKTKFADVYQLIN
                     AISRKDKRAAHKVLGELESSGEEPLSILGLISWRFRLIWKAKELQDRKLGQSEMLKEL
                     KISPGQLYYLSEDL"
BASE COUNT         1538 a         2153 c         2017 g         1806 t
ORIGIN      
        1 tcaggaatgc aaaaagtata agtattataa tcatcggagt gatccaccac catttgttct
       61 gtatgaaaaa ctgtagcagc tcacccacta tgcccattct ttcggtcagt ccttttataa
      121 atgacatatc gtatccctcc ggattgcccc taaattatat cagcttttta aaatttatgt
      181 aataccgtgc cggtcagggc agcttgtcct tgtccacgat gtaatttccg agcacgagca
      241 tgtccattcc gctcttgctg aaagagttga acgcctcgac aggtgtattg actatcggct
      301 cacccttgag gttgaacgaa gtgttgagca cgaccggcac ccctgtcgcc tgtccgaacg
      361 tctcgataag gctgtagtat ttcggattta actccttcct caccgtctgg agcctccccg
      421 tgccgtccac gtgcgttatg gcgggcagca cgtcgcgctt gttctcgtgt acgtccgtaa
      481 cgtagagcat gaacctcgcc ggatagtgct tttccggatg gtcgagcgcg aaataatccc
      541 cggccttctc cacgagcaca gaaggcgcga agggacggaa cggctccctg aatttaatct
      601 tcacgttcac tatgtccttc atgtcggccc tgcgggggtc ggcgagtatg ctcctgttgc
      661 cgagagcgcg gggaccccat tcgaacctcc cctggtgcca gcctatgacc ttccctccgg
      721 tgagggcgtc cgtgacgcgg gcgatgagct tctggtcgtc ctcgatcttt tcataaggga
      781 tgttattctc cgtgaggaac tcctccgtgt cagaggcgct atgttcttcg ccccagtagg
      841 catgctccat gacgaacttc cggggctggc cgagcagagc gtggtagccg tagagggccg
      901 cgcctacggc tcctccgccg tctcccgccg cgggctgtat gtatatttcc tcaaacggcg
      961 tctccgacag tatcttcctg ttggcgacgc tgttcagagc gacgccgccc gccatgcaga
     1021 gcttgtttat acccgtttcc ctgtaagcgt agttcgccat cttgagcatt acctgctccg
     1081 tcacgacctg aatgctggcc gctatgtccg cgtagaactg gttccgcttt ccgagctcat
     1141 cgaaatccgc gggcttttcc ccgaagtagg acggatagcc ggtcgaaggc gtgaagaaat
     1201 gcgccttctg gtccctcggg tcgccgaata gcttctcgaa cttctttttg tacgtttttt
     1261 cggaggagta atggaatgag aagtagtcca tgtcgagctc gaacccgccg tcatcgtcca
     1321 catggacgag ctcgtatatc ttgtccacgt aattgggcct tccgaagggg gccatgccca
     1381 tgaccttata ctctccctcg ttcacctcga atccgaggaa cgcggtgaag gcgctgtaga
     1441 ggagtcccag ggaatgcggg aaccttattt ctttaatcag cttgatgtcc gtgcccctgc
     1501 ctacgccgag cgcggctgta gtccattcgc ccaccccgtc cacggtgagg atcgccgcct
     1561 cgtcgaacgg agagcaataa aaggtgctcg ctgcgtgcga gaggtggtgt tcgctgaaaa
     1621 gcaccttcga cgcgggcacg cccagcttct tgagcaggag gtgcttgatc cagagcttgt
     1681 ccccgagcca cgtgaccata gcctccctga agagcttcat cgagcgcggg aaggtctgca
     1741 tggagcacag gagcagcctc tcgaacttga tgaagggctt ctcgaagaac atcacgtaat
     1801 cgatgtcgcc cgcttcgatc ccccccgccg cgagacaaaa cccgatcgcg ttctcaggga
     1861 actcgtagtc gtgctttata cggctgaacc tctcctcctc ggcggccgct atgaggacgc
     1921 cgtccttaag gagcgcagcg gcggcgtcgt gaaagtaaca tgatattcca aggatgttca
     1981 tatattctcc gttcttctac cgtgccgaca ggggctatcc gccgtcagaa ttgtctcacc
     2041 gccttctcca tgggagtaat gtccggattc tcttccctcg gctgccagcc ttttttcgcg
     2101 ttctttttga tctccagggg atcggtgaag actttaacca ggagcgcgaa aggcatcagc
     2161 aggatgagat agaaaatggt gagtattacc ctcgcgttaa aatcccctat cttcctcgct
     2221 atctttttcc agccgtccca tatcttcctg agaaggctct tttccttttc ttccttcttt
     2281 tttcgctgga ttgtgctcat cttgataaat caaacggttc agccatcgac tggccgtttc
     2341 ctcctttggt ttctactttt gtgactactg tcagttgtta attaatccga cgacttagcc
     2401 cacgaaagaa cttaaaaaat acccgacgat ccgtcttttg aacatctaaa tatacattta
     2461 tgtgcaatat tccggtaatt aaagaaagtc acagggcaat tagtgtattt acattgacag
     2521 acccgccggg cttgaatgat acgggcggct ttttcatagc cgcacctgtt atcagtcata
     2581 ccggcctccg gcgcggaggt ctctttgcgg tcagcctacg gtatcggtgt tctctatctt
     2641 atatgtaagc tccctgatcc tccacgagcc tccggtccac gaaagggtcc agtttatctg
     2701 ccccttcgag ctctttagcg cgcccgtgcg ctggtccttg aaggttatga agaattctcc
     2761 ccggacgtac ccgagagagt tctcgacgga tatcctgctg actttaacgt cgtatttcat
     2821 tatgtccaga ttggaaaaat tagagctgta ggagctgagt accctggata tgctcacccc
     2881 gttttccacg gcgtccggca taaagagggc cttcacgcgg tcgatgtcct tgttcttgta
     2941 tgccgatacg taatcggtta cgaagccgta aacggagtcc ctgtcaggcg gagcagcggg
     3001 gggaatgggt tcggggggac gtactgaggt gtcccgtacg gctaccgcgg aagtcgcgag
     3061 cttggggttg aaatcggatg ttatgtcagt ttttacggga ggtttcaccg gagcctgtgc
     3121 cttcgctacg acgggcgcgg gagtgatttc cctcttcggc cgagtgaccg gaacgggtac
     3181 gggctccgcc tttttcaccg ctacctcggt tacgggtttg ggaggtataa cgccttcgcc
     3241 cgagatcaat atcttttcgc ctatgcggag cttgctgtcc gagaggccgt tcagactcac
     3301 aagctcgccc gtggtcttgc cgaacttgtg cgctatggtc cagagtgagt cgccctgcct
     3361 gaccgtatag tattctccgg ccgagggggg attctctttc tcgggaacgg aaatttccgt
     3421 gagctcgccc ttcggtttca cgggagccgc gggtttctcg gccagtttta tatcaggcgc
     3481 tttttcagtc tcttttattt cgctctcggg ttttatttca ctaccgggtt tttgctcgtc
     3541 ttttacagcc ccgctcttct cggctgtcag gacctcttgc ttaggagagg gttcggcgct
     3601 cttcttcacg gtctcggcgg ccgggggagc ctcgggcgac tttcccgcct ccttgccggc
     3661 cggcgccgga gcctcatccg actttgctgc gagcgtttct tttaccgcgg gttttacttc
     3721 cccgggaacg ggctcagtcg gctgagtctc cttgtcagac acgtccggcg tcacttcagt
     3781 tatctctatc gtcttttcgg ccttcggctc aagggccgcg ttcccaaact cctcggcggg
     3841 caccgtctcc gtaacatctt cagaatcggt cttttctacg ggagcggtct cgtcagccgg
     3901 ccctgcatcc gctaccactg tattctccgc gggagagggc atttcctgcg ttacggcagc
     3961 cgatgttgca tctttttctt tcactgcaga cttttcaccg gtcctttttg cctgctctat
     4021 gggcttgatc tctgaaatct ttttatcgac tttctcggcg tccaggtaat cgcccttgta
     4081 cacggtgttg ggcatgtccg gggcctcgaa ttccctgacg agcctttcct tttcctcagg
     4141 cgactggaat atcagcccgg agcccgaggc gtacataacc acgagaaaaa cgaagaccat
     4201 gagcgcgccc gcataatagt atttcttcat ctcgtgctgc gggacgatga caggggtcgg
     4261 cgggaggtgc ttgttgtcgt aagcttccct cttcgaggcg ctgctcagca cctcatacgc
     4321 ttcattgatt tttttcgacg tatcgagtcc ctctgcgccg gctttatccg gatggtgggc
     4381 cttcatgagc tcgatccagt tccgcctgat ttcctcgtcg gtcgcgctcc gggatacgtt
     4441 cagtatcttg tagtagtcga gggagtcggt ctggaagctc tggtagtagg gcagaaggaa
     4501 attgaggacc gatttcgcct gctctataac gaagtcttcg ctcagcttcc gcctttgcgc
     4561 aatgttcttc aatagcttgt gattcggaga tcgctcgccc agcgatatgt ccttcagtat
     4621 atggtagaag tagatcctgt cgagggagta tctttcgacc agctccctga tgtccttggc
     4681 gtcgatcagc tcggatacga tacgctcgta ctttctttta tcgagtatta tcataattcc
     4741 ccttatccgc cgctgccgcc ggagacactc gagctcccgc ggacggaggc attactttat
     4801 catatttaga gaatttttcc cgcatgcggg acgtgaacga ggatccggga atggaacccg
     4861 ccgggccgga acggaatgtg cgtcctctac gagaaacttt cagggttctt atccccggcg
     4921 tatcaggtga gaacgatgaa gaataaattc cggcacctcc gcaatagatt ccatcaccct
     4981 ttaccggacg cagcgatcaa gcttcaacat aattatcatg tatccgagcc gattgggcaa
     5041 gtattttgag aaaattcctc ccggacctga ccgctgagcc gttatttgag gcgcgggcgc
     5101 gtgccgctgc ctgagccgct atgggcggag cggcgccgga gcgcccgaat ttgacaaaac
     5161 agtatttccc gaaagaatta aggggcaaaa aatcatccgg aggcatggaa ttggaacgca
     5221 cactatcgat aatcaagccg gacggggtcg aaaaaaacgt tataggggaa gtcataagca
     5281 ggttcgagaa gaacgggctc aggattgccg ccctcaagat ggtaaagctc acgaaagagg
     5341 acgcccgggg cttctacata gtgcataagg agaggccctt ctacgggagc ctgaccgatt
     5401 tcatgtcccg gggccctgtg gtgctgatgg tgctcgaagg cgagaacgcg atcgcaaaga
     5461 acagggagat tatgggggcc acgaaccccg cccaggcggc agagggcacg ataaggaagg
     5521 acttcgccac cagcatcgag gagaacaccg ttcacggctc cgattccccc gaatcggccg
     5581 ccttcgagat cggctacttc ttcaacgcgc tcgagctctg cgggggatag accacttcag
     5641 ttgtgcaaca gcggtccaat cccgttcgtc ccgaacatat atttccttcc cccttgaggg
     5701 acggttgcaa gcaagcgcgc aaacggatgc cacggaacag aggcacagaa gcacattaca
     5761 tgtctactca atgctgccta atacaaaaca gggttagtat gggggtgata cagttcattc
     5821 attaatcaaa gaaaacccct cacccgcctc cctcatctcc gccccgaaga cctcgccgaa
     5881 gcttctgacg aaagcccgcg tgacggctcc gaaatccatc tcctcgtcga gtatctccct
     5941 gagagaggtc atcctcaccc catccaggcc gcagggtatg atcatcgaga aatagtcgag
     6001 gtccgtgttc acgttgaacg cgcagccgtg catcgttatg ccgttcctga cggccacgcc
     6061 gacagaggcg atcttgcccc cgtacgccca gacgcccggg aacttctcca tggtcacgcc
     6121 ttttagatcg taatccccga gcgtgcggat tatagtctct tcgagcgcgc gtacgtaagc
     6181 cctcgcgccc atgccgaaag cgcggaggtc tattatcggg tacgccacga gctgaccggg
     6241 gccgtggaac gtgacgtcac ctcccctccc ggtcttatat gtctcgactc cccgcttttc
     6301 aagctcctcg ggaggtagca taatgtttcc gccccggccg aacctgccgt cggtgatcac
     6361 gggactgtgc tctaaaagaa gtagataccc gcccgcgccg ggaccctctt tgcggagcgt
     6421 ctcgcggcgc tcgttctgga gcgcaaggcc gtcagcgtaa ctcacgacgc cacggtattc
     6481 ccatcttatc acgcgcttcg tttccgaatc catacgccta acagtctaaa cgataagcgg
     6541 cccctttcca gagcgcttgt ctccccggac gtactcgcat atactcaccc tgtatccgct
     6601 ccggggcgaa tgaaaggtta agcatccccg gccggctatc ccgatatgaa acagaaatca
     6661 ggcggaaaat catccgcgca ggacttctca agctacatcg cctcgggaga tcccgcgccg
     6721 gtgctcgttt tcacgggcgg ccaggcgttc ctcatcgaaa aggcggtcac ggaactgagg
     6781 gaaaggctcc tcggcccttc gggggacatc aatttcatca cgttcagcgg ggagagcgcc
     6841 tcgggaaggg agattgccga caacgcgagc acgtacccca tgttctctaa aaagaagctc
     6901 gtcgtggtga agaacgccga caaattatcg gcaaaggagc ttacagcact cgaaccgtac
     6961 atttcatcgc cttccccttc cgcgtgcctc gtgcttgttt tcggggatgg taagaagccg
     7021 aagctcggcg gggggaagca cgtcgccgct ttcgatttct ctatggaaaa gggcagcgcc
     7081 ccctcggccg ttagggagga agcccggaag ctcgggtacg acatcacacg cgcaggcgca
     7141 gaggcgctaa tcggcctggt gggggaagac cttcaggaga tacacaacga gctcgtcaaa
     7201 atatccctgt ataagggcga taagaagacc ataggcccgg aagacgtgga agcgctcacg
     7261 aagaagacca agttcgccga cgtttatcag ctcataaacg ccatatcaag gaaggacaag
     7321 cgcgcggccc acaaggtgct gggcgagctc gaatcatccg gggaggagcc cctatcgata
     7381 ctcgggctca taagctggag gttcaggctc atctggaagg cgaaagaact gcaggacagg
     7441 aagctcggcc agtccgagat gctgaaggag ctgaagattt ccccggggca gctctactac
     7501 ctgagcgagg acct
//
LOCUS       DASSJH010000197         5521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1019505, whole genome shotgun sequence.
ACCESSION   DASSJH010000197 DASSJH010000000
VERSION     DASSJH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019505"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..843
                     /gene="truA"
                     /locus_tag="VFJ67_04205"
     CDS             109..843
                     /gene="truA"
                     /locus_tag="VFJ67_04205"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7289620.1"
                     /translation="MRNIKITIEYDGSGYVGWQRQPSGPTIQETIETSLATMTGERVK
                     LLGSGRTDSGVHALGQVASFRTGTSLSPSDIQKGLNSLLPRDIVITGAEEAEPDFHAQ
                     LSAKSKTYIYKILSRPYPSAILKDRAWFIPYPLKARLMDEAARSLVGEHDFRAFAQAE
                     ATVKSTVRTVLAARITKRKDLIEFSIEADGFMKRMVRLIVGTLAQVGKEKITPSQFRE
                     ILESGEKTKHVHAAPARGLYLKEVRY"
     gene            907..1665
                     /locus_tag="VFJ67_04210"
     CDS             907..1665
                     /locus_tag="VFJ67_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7289621.1"
                     /translation="MKFLRFETRGKETLYGTPDSGGIKEIRGDIFVRFEAAGRTFDPA
                     EVRLLPPVMASKIVAVGLNYNDHAAEMGRELPEEPRLFIKPSTSLIAHGDKIIYPKHM
                     SSHVDYEGELALVIGKKAKHVEESDALGYVLGYTCLNDVTARDLQSKDIQFTRAKGFD
                     TFAPVGPVVETELDPGDLEISTYLNGERKQHSSTKNLLFSVPRLVSFISKVMTLLPGD
                     IISTGTPSGVGPMKQGDKVEVEIEGVGKLTNYVV"
     gene            complement(1687..3639)
                     /locus_tag="VFJ67_04215"
     CDS             complement(1687..3639)
                     /locus_tag="VFJ67_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7289622.1"
                     /translation="MSTKKTAKKSRKNTATPAQRPPVAEHVPDSPLLRIIRHRALAAV
                     FLFVVSFSVFIPTLNNGLVWDDLTFVESWGPRLKDVPLGLNLIFPSRNEEAKSSKYYR
                     PVFSSSLIIDAKIWGNSNFGFHFTNVFIHSVSTVLLYLLILLLFKEFNRGTGESEAFL
                     GSMLFAVYPIHVESVSFISARGDILAAMFLLLCLIFYILSYRNNFFILLAGVSFYLSF
                     LSKEVAFSFPVIILGFDLISRRLFSRASIVKYIAICILVIIYFYLRWGSIGNSLNILN
                     SAVYRETGSSPGVLEFIPMFLGAYLFYNWKLIFPYDLNHFIGAIPAGDALTIIISLLL
                     VVGVISVFIISFRKKENITAFSLLWIFATLGPAVLIAIYPLAATRYAERFLYVPSAAF
                     CLLLGYVIVRGGRLTGRRWAAAAVGGLLCASYVIVTVKDQKIWQDEITFWEAVIKKSP
                     DQLVPRVNYGEALRRYGRADEAIHQHSALLDLNIKGSQRGKSYAAISLALDYIDKGEY
                     RKAEEYLDTAVKYDPGIEAKYNYYRGYIYMKQDDIYSARPYLEKAIELQPVYPQALYL
                     LGVFYWSEAEKNYDMYGLCIRSLERAVEQDKGFIAARLLLAQAYNAVGEKEKARNQIE
                     TVLRLSSDPNYLRQAQSILGAINSYQ"
     gene            complement(3839..>5521)
                     /locus_tag="VFJ67_04220"
     CDS             complement(3839..>5521)
                     /locus_tag="VFJ67_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7289623.1"
                     /translation="KEAKTNRYFRPVYFASLVIDYRMWGAAPFGYHMTSIILHAVSTV
                     LLYFLVLLLFGEFKRGPGGSEAFLASLLFAVYPLHVESVSFIAARGDILAGMFFLMCL
                     IFYILSYRRFFYILPAAFAFYFSFLSKEVALAFPLIILGYDFISGRLLARTNIFKYMI
                     IGLLIVLYFYVRSGSFMHFLDVLYQNSLRETEGTAGIGEVVTLFLGTYLFYVEKLLFP
                     YNLNHFIGTIRGGTAFYIIVSVLLIAAVAAVFVISIKKRENITAFSLIWIFAALGPAV
                     MIAIYPLAITRYAERFVYIPSMGFCMLLGYLIVRGGRLTGRRWAAVAACGLLCASYLV
                     VTVKGQEVWSDEITFWEAAVRKSPDQIIPKVNYGEALRQAGRVDEAIRQHTAALDPRI
                     ESTGRGKAMAASSLAADYIQKGDYTNAETYLRAALEYNPATEGQYYYHMGLIRFKKND
                     TEEAANYLRRAVEVDPKNAKSYYLLGVIYALEAENRKSADDYRLAKEALTNSLKYDEG
                     LVSARILLAQVYLALGDRARAREQAEAAIRYTSDPVTIRQVKSMLNAIDAGR"
BASE COUNT         1442 a         1454 c         1462 g         1163 t
ORIGIN      
        1 gataacgatc gtgtacacga taatcctcgt gctcgcgaac ctgatagtgg acgtgctcta
       61 cgtcattctc gacccgcgga taaaattcga caagggcggc atatagaggt gaggaacata
      121 aagatcacga tcgagtacga cggctccgga tacgtgggct ggcagagaca gccctcgggg
      181 ccgacgatac aggaaacgat agagacgtcg cttgcgacaa tgaccgggga gagagtaaag
      241 ctcctgggct cgggtaggac ggattcaggc gttcacgcgc tcggacaggt cgcaagcttc
      301 aggacaggga cttcgctcag cccctctgac attcagaagg ggctcaattc cctcctcccc
      361 agggacatag tgatcaccgg cgcggaagag gcggagcccg atttccacgc ccagctctcg
      421 gcgaagagca aaacatacat atacaaaata ttgagccgtc cctacccctc ggcgatacta
      481 aaggacaggg cgtggttcat accctatccg ctcaaggcgc gtctgatgga cgaagcagcg
      541 cgctctctcg taggagagca cgacttcagg gccttcgccc aggcggaggc gaccgtgaaa
      601 tcgaccgtga ggactgtgct cgccgcccga ataacgaaac ggaaagattt gatcgaattc
      661 agcatagagg ccgacgggtt catgaagaga atggtcaggc ttatcgtcgg gacgctggcc
      721 caggtcggga aggaaaagat cacgccttcg cagttccggg agatactcga atcgggagag
      781 aaaacgaagc acgtgcacgc ggcccccgca cgcggtcttt atctcaagga agtcaggtac
      841 tgattcgtgt ataatcccgt gctctgacgc tcaagtaaca aaaccccatc acgagaggta
      901 acggatatga aatttctgag attcgagaca cgcgggaagg aaaccctgta cgggacgcct
      961 gattcgggcg ggataaagga gatcaggggg gatatattcg tgagattcga agcagccggg
     1021 cgcacgttcg acccggcgga agtgaggctc ctccctccgg tcatggcgtc gaagatcgtc
     1081 gccgtgggtc tcaactacaa cgaccacgcc gcggagatgg ggagggagct gccggaagag
     1141 cccaggctgt tcataaagcc gagcacctcg ctcatagcgc acggggataa gataatctat
     1201 ccgaagcaca tgtcctcgca cgtcgattac gaaggggagc tggcgctcgt cataggaaaa
     1261 aaggcaaagc acgtggagga gtcagacgcg ctcggttacg tgctcggata tacctgtctg
     1321 aacgacgtta cggcgaggga cctccagtca aaggacatac agttcacgag ggccaagggc
     1381 ttcgacacgt tcgccccggt gggcccggtg gtcgagaccg agctcgatcc gggggacctc
     1441 gagatatcga cttatctgaa cggcgagagg aagcagcatt cgtcgacgaa aaacctgctg
     1501 ttcagcgtgc ccaggctcgt gagcttcata tcgaaggtga tgacgctatt gccgggagac
     1561 ataatatcga cgggcactcc ctcgggcgtg ggaccgatga agcagggcga caaggtggaa
     1621 gtagagatag agggtgtcgg gaagcttacg aattacgtgg tctaacgaac tcgccagcga
     1681 agggcgtcat tgatatgagt tgatcgcgcc gagaatcgat tgcgcctgcc ggaggtaatt
     1741 cgggtccgac gaaagcctga gaacggtctc aatctgattc cttgcctttt ccttctcccc
     1801 gacggcgtta tatgcctgag cgagaaggag cctcgccgca atgaatccct tgtcctgttc
     1861 cactgccctc tcgagcgatc taatgcagag cccgtacata tcgtagttct tctccgcctc
     1921 cgaccaataa aacacaccta aaagataaag tgcctgcgga tacacgggct gtaattcgat
     1981 cgccttttca agataagggc gggctgagta gatatcgtcc tgtttcatat atatgtagcc
     2041 cctgtaataa ttgtacttag cttcaattcc ggggtcatac ttgacggcgg tgtccagata
     2101 ctcttcggct ttccggtact cgcccttatc tatataatca agtgcgagag agatcgcggc
     2161 gtaactcttt ccgcgttggc tgcccttgat gtttaaatcg agcaaggcag aatgctggtg
     2221 aatagcctca tcggctctgc cgtatctcct gagagcttcg ccgtaattga ccctgggtac
     2281 aagctgatcg ggggacttct ttatcacggc ctcccagaat gtaatttcgt cctgccatat
     2341 cttctggtct ttaacagtca cgatgacata agaagcgcac agtaatccgc ccacggcagc
     2401 ggccgcccat ctcctccccg taagcctccc ccccctcact atcacgtatc cgaggagcag
     2461 gcaaaaggcc gctgaaggga cataaaggaa cctctcggcg tatctggttg ccgcgagcgg
     2521 atatatagct atcagtaccg cggggccgag cgtagcgaaa atccagagga gagagaatgc
     2581 tgtgatgttc tcctttttcc tgaacgaaat aataaaaacc gatataaccc caactaccag
     2641 aagtagcgaa ataataatag tcaaagcgtc accggcaggt atagccccga tgaagtggtt
     2701 caggtcatac gggaagataa gcttccagtt atagaagaga tacgccccca gaaacatggg
     2761 gataaattcc aagacgcccg gtgaactgcc tgtttcacgg tatacagccg agttcagaat
     2821 attgagagaa ttcccaattg atccccacct caaataaaaa taaataatta ccagaatgca
     2881 gattgcgatg tatttaacga tgcttgcgcg gctaaacagc ctcctgctga tgagatcgaa
     2941 gccgagaatg atgacaggga atgagaacgc cacctctttt gagagaaagg aaagataaaa
     3001 tgaaacccca gccagtaaaa tgaagaaatt atttctgtat gacagaatgt agaagatcaa
     3061 gcacaggagg aggaacatcg cggccagtat atctcccctt gccgaaataa aagatacgga
     3121 ttcgacatgg ataggataca ccgcgaaaag catcgaaccg agaaaagcct cgctctctcc
     3181 agtcccccta ttaaactcct taaagagcag aagaatcagg aggtaaagca gcaccgtgga
     3241 aaccgaatga atgaatacgt tggtaaagtg gaatccgaaa ttagagtttc cccatatctt
     3301 ggcgtcaatg atgagcgatg aagagaatac aggtctgtaa tatttactgc tcttagcctc
     3361 ctcattccta gaggggaata tgagattcaa acccagaggc acgtccttga gccttggccc
     3421 ccaactttcc acaaacgtta aatcgtccca gacgagaccg ttgttgagtg taggaatgaa
     3481 tacggagaaa gagactacga acagaaacac cgcagcgagc gccctgtgcc tgattatcct
     3541 caatagagga ctgtccggga catgctccgc cacgggcggc ctctgggccg gtgtcgcggt
     3601 attcttccga ctcttctttg cagttttctt ggttgacata ccctcaactg tagctaattt
     3661 ttttaggcag cttttccgag tccatcatga cagagcagta attcatgcat atctattata
     3721 caacaggctt ttacagcaga agaaaccgta aaccgttgcc cgaggtaagt tacgcccgta
     3781 ctgcagagag caaacctata agcttgagta acccgcttgt gaattccgca taaatgcgtc
     3841 aacggcccgc atcgatcgca ttaagcatcg acttcacttg tcttatggtt accggatcgg
     3901 aagtataccg tatagcggcc tcggcctgtt cccttgccct cgccctatcc ccgagggcga
     3961 gatagacctg tgcgaggagg attctcgcgc tcacgagccc ctcgtcgtat ttgagtgaat
     4021 tcgtgagcgc ctctttggca agcctgtaat cgtcggccga tttccggttt tccgcctcca
     4081 aggcataaat cacccccaaa agataatagg acttcgcatt cttaggatcg acctcgaccg
     4141 ctctccggag atagtttgcg gcctcttccg tatcgttctt tttaaaacgg atcagcccca
     4201 tgtggtaata atactggccc tccgttgcgg ggttatactc aagcgccgcc ctgagatatg
     4261 tttcggcgtt cgtgtaatcg cccttctgta tgtagtcggc agcaagcgag cttgcggcca
     4321 tggccttccc ccgtcccgtg ctctctatgc ggggatcgag cgcggccgtg tgctggcgta
     4381 ttgcctcatc caccctcccc gcttgcctga gcgcctcgcc gtaattgacc ttggggatga
     4441 tctggtcggg ggacttcctc acggctgcct cccagaacgt aatctcgtcc gaccatacct
     4501 cctggccctt taccgtaacg accagataag aggcgcatag caatccgcac gcggcgactg
     4561 ccgcccacct cctccccgta agccttcctc ccctcactat cagatacccg aggagcatac
     4621 agaagcccat agacggtata taaacgaacc tctccgcgta ccgcgttatc gcgagcggat
     4681 atatcgctat catcaccgcg gggccgagcg cggcgaaaat ccagataagc gagaacgcgg
     4741 tgatattctc tcttttcttt atcgaaatta cgaataccgc cgcgaccgcc gctattaaca
     4801 gaaccgaaac gattatatag aacgcggtcc cgccgcgaat cgttccgatg aagtgattaa
     4861 ggttgtaagg aaaaaggagc ttctcgacat agaaaagata agtgccgagg aagagcgtaa
     4921 cgacttcgcc gatacccgct gtcccttccg tttcacgtaa gctgttctga taaagcacgt
     4981 cgaggaaatg catgaacgac ccggacctca cgtaaaaata caaaactatg agtaagccga
     5041 ttatcatgta tttgaatata ttggtccgcg cgagcagcct cccgcttatg aaatcgtagc
     5101 ccagtattat caggggaaag gcgagggcca cctctttcga gagaaaggaa aaataaaaag
     5161 cgaacgcggc cggcagaatg tagaaaaacc ttctgtagga caatatgtag aagatgagac
     5221 acatcaggaa gaacatcccc gccagtatgt ctcctctcgc ggcgataaag gaaacggact
     5281 cgacgtggag gggatagacg gcgaagagaa gcgaggctaa aaacgcttcg ctcccgccgg
     5341 gaccccgctt gaattccccg aagagaagga gtacgaggaa atacagcaga accgtagata
     5401 cggcgtggag gataatgctc gtcatgtgat acccgaacgg ggcggcgccc cacatacggt
     5461 agtctatgac gagcgaggcg aagtacacgg gcctgaagta tctattggtc ttggcttcct
     5521 t
//
LOCUS       DASSJH010000198         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_807328, whole genome shotgun sequence.
ACCESSION   DASSJH010000198 DASSJH010000000
VERSION     DASSJH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807328"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFJ67_04225"
     CDS             complement(<1..337)
                     /locus_tag="VFJ67_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289624.1"
                     /translation="MIFVMGTGLYLLLNFFYLHGFGVFFVAVFALLAVCLLLFAVLTV
                     KVDGSDVSLRFGIGIIRKKFPVSGIGSALIVRNPWYYGWGIRKTPDGWLYNVSGLIAI
                     ELHMKNGEKY"
     gene            complement(406..1074)
                     /locus_tag="VFJ67_04230"
     CDS             complement(406..1074)
                     /locus_tag="VFJ67_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289625.1"
                     /translation="MTGKRFSKREAIEFGWHTTRANLGFFIVFLVVSMLISFFFSGFA
                     DLFEKRLLLLSAIFNLGYIFLTIVINLVGIKIALKFCDNDRRALAEVISFTPRLFLKF
                     AAGYILYGLLVAAGIILLVVPGLIFMVKYQYVIYFIADKNADVGEAFKKSSAITSGVK
                     WELFVFLILLSLINVAGIMCFFVGLLVSIPVTMLAAARVFRKLSGSVKEEAINPFQAP
                     GQTL"
     gene            complement(1144..1851)
                     /locus_tag="VFJ67_04235"
     CDS             complement(1144..1851)
                     /locus_tag="VFJ67_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7289626.1"
                     /translation="MSLVVLRDGLRVIDLGCGTGELTRMLADRLPASDVLGIDSSPEM
                     LEQAKSKEREGLRFELRSREDVSGGGWDLIFSNAALHWVDDHEKLIPALFNFLGPGGQ
                     IAVQLPSNHNHETQLLVHEIAKEPPFASAIGGWVRKSPVLSINAYAEALYKCGGMDIN
                     VFEKVFPHVLNDSDAIADWLAGTMMRPYLSRLSAELQARFMDTFKERLRRIYTRAPVF
                     YPFRRIFFSALKTNLHG"
     gene            2053..2439
                     /gene="crcB"
                     /locus_tag="VFJ67_04240"
     CDS             2053..2439
                     /gene="crcB"
                     /locus_tag="VFJ67_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET7289627.1"
                     /translation="MTNLIFIAIGGAAGAVLRYSVSGFVHGYANGLFPWGTLVVNLSG
                     CLVIGILWQMFENLSFSPHLRSFMFIGVLGAYTTFSTYGLETFNLMREKEIGYVLLNF
                     LGSNILGIGMVFLGFIAARLILNIAR"
BASE COUNT          645 a          678 c          588 g          615 t
ORIGIN      
        1 ggtatttttc cccgttcttc atgtgtagct cgatagctat caggccggac acgttataga
       61 gccatccgtc gggggtcttc cttatacccc atccgtaata ccagggattc ctaactatca
      121 aggccgatcc tatcccgctt acggggaatt tcttgcgtat gatccctatg ccgaaccgaa
      181 gcgacacgtc gctcccgtcg accttgaccg tcaatacggc gaacaggagg agacacaccg
      241 cgagcaacgc gaagaccgcg acaaagaata caccgaaccc gtggagatag aagaagttca
      301 acagcaaata caggcccgtg cccatgacga aaatcattag atacccgatt tgcgtgtgtt
      361 tgtagatacg gctcaatttc ttaatctacc cctttatttt cggattcaga gtgtctgacc
      421 gggcgcctga aacggattta tcgcttcctc tttcacggag cccgacagct tcctgaaaac
      481 tctcgccgca gccagcatgg tgaccggtat cgagaccagc agcccgacga agaagcacat
      541 aatacccgcc acatttatca gcgataataa tatcaaaaac acgaacagct cccacttgac
      601 cccgctcgtt atagcagagc tctttttaaa cgcctcccct acgtccgcat tcttgtccgc
      661 gatgaagtag atgacatact gatatttgac catgaagatg aggccgggca ctaccagcaa
      721 tattatgccc gcagcgacaa ggagaccata gagaatataa ccggcagcga attttaaaaa
      781 caacctgggc gtgaatgaaa tcacctccgc tagcgctctt cggtcgttat cgcagaactt
      841 gagtgcaatt ttaataccga cgagattaat cactatggtc agaaagatat atccaagatt
      901 gaaaatggcg gataataaca gcaggcgttt ttcaaacagg tccgcaaaac cgctgaagaa
      961 aaaacttata agcatcgaga cgacgaggaa tactataaaa aagcccagat tcgctctcgt
     1021 cgtatgccat ccgaactcga tcgcttccct ttttgagaac ctctttcctg tcacaagcga
     1081 ctccttcccg aacccagctg cggctaattt ttcgccgaca gtccgaactt taaattccta
     1141 ttgctatcca tgcaaattcg tcttcagcgc cgaaaagaag atgcgtctga aaggatagaa
     1201 gacaggggcc ctggtataaa ttcttctcaa cctctctttg aatgtatcca tgaagcgcgc
     1261 ttgcagctcc gcagagagac gcgagaggta cggcctcatc attgtacctg ccagccagtc
     1321 cgcaatcgca tcggaatcgt tcagtacgtg ggggaatacc ttctcgaaaa cgttgatatc
     1381 cattccgccg catttgtaaa gcgcctcagc gtacgcgtta atcgacagta ccggggattt
     1441 ccttacccac cccccgatgg cggaagcaaa cgggggctcc ttcgcaatct catgtacgag
     1501 gagctgagtc tcgtggttgt ggttagaggg gagctgaacg gcgatctgac ctccgggacc
     1561 gaggaagttg aacagcgccg gtatgagctt ctcgtggtcg tccacccaat gcaatgcggc
     1621 gtttgaaaaa atgaggtccc atcccccccc ggagacatcc tctctcgacc ttaactcgaa
     1681 cctgagcccc tcccgctctt tggacttcgc ctgctccagc atctcgggtg acgagtcgat
     1741 cccgagtaca tcgctcgcag gaagtcggtc cgccagcatt cgggtaagct cacccgtgcc
     1801 gcatccgaga tcaatcaccc tgagaccgtc gcggagtacc actagactca tcaggtcttc
     1861 gaaaggcgct gacctctctg acctgaattt atgatatagc tcggggttcc aggacattat
     1921 tcggctttcg cttccctccg gtgattaaag ttacttaaat aaacgtggaa attccataaa
     1981 agtacaaata atcccgcata tgttatcatt ttcttccatc cgttgatcat ataatcacat
     2041 ccgggggcga taatgacgaa tctgatattc attgcaatag ggggcgcagc cggagccgtt
     2101 ctgagatatt cggtatccgg tttcgttcac ggctacgcga acggattgtt cccgtggggg
     2161 accctcgtcg tcaacctgtc cgggtgtctc gttatcggga tcctctggca gatgttcgag
     2221 aacctttctt tctctcctca tttgaggtcg tttatgttca taggcgtgct cggggcctat
     2281 acgacgttct ccacgtacgg actcgagaca ttcaacctca tgagagaaaa agaaatcgga
     2341 tatgtgctac ttaattttct cgggagcaat atcctcggca ttggtatggt gtttctcggc
     2401 tttatcgccg caaggcttat attaaatatc gcgaggtagg aggcgtatat gaatatcttc
     2461 aaagaagaga tgcttgtcag gatattcatc ggcgagagcg acagttacga cggcaaatct
     2521 ctctat
//
LOCUS       DASSJH010000199         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_374714, whole genome shotgun sequence.
ACCESSION   DASSJH010000199 DASSJH010000000
VERSION     DASSJH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374714"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFJ67_04245"
     CDS             <1..675
                     /locus_tag="VFJ67_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7289628.1"
                     /translation="MEGYGLTEIGQCTWMRPGEPFRVGSCGKEAPGYEIKIGDPETDE
                     EVPRGYVGEIIVRPRTPNIMLHYYNKMPEKTVQDFRNFWFHTGDAGMMDKDGYIYFVD
                     RVKDYIRRRGENISSFEVEKIVSSHPEIEECGVIGIKAESGRYAEDEVMIVVVCRKGK
                     KIDPHKLMEFLEPKMPHFMLPRFIRFETTLPKTGTERVQKNKLRESGITKDTWDREKS
                     GYKVKR"
     gene            770..1036
                     /locus_tag="VFJ67_04250"
     CDS             770..1036
                     /locus_tag="VFJ67_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289629.1"
                     /translation="MSRIMKASLPFLAGVLFAAIIMGSFNIFREAQAQPGYQPNMEQA
                     LGSLHQARISLEISSPDKGGHRVRALELVNQAIEQVRLGMYFAE"
     gene            1231..>2169
                     /locus_tag="VFJ67_04255"
     CDS             1231..>2169
                     /locus_tag="VFJ67_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289630.1"
                     /translation="MRHLILALLMPAVAMLACAKAQYSPPDLGGIYTQAAMRSHEEGN
                     PVIVIPGILGSKLRDSETGQLVWGAFDGGEADPETPEGARLIAIPMKEGVPIDELTDS
                     VRSDGALDRVKVKLFGLPLQLNAYINILSTLGAGGYLDQSLASNKLNEIDYGKEHFTC
                     FQFDYDWRLDNVENAKRLKKFIEEKKEYILGEYKKRGINKDDVKFDIVAHSMGGLLTR
                     YFLMYGDAGLPRDGSKPVVTWAGAEYVEKVIIVGTPNAGAVGAVETLVKGRDIGPFLP
                     KYEPAIIATMPSLYQQLPRTRHGEVVDENGQRLDLMD"
BASE COUNT          556 a          572 c          650 g          391 t
ORIGIN      
        1 atggagggtt acggtctcac cgagataggc cagtgcacgt ggatgaggcc cggagagccc
       61 ttcagggtgg gctcctgcgg caaggaagcg cccggctacg agatcaagat cggagacccc
      121 gagaccgacg aggaagtgcc gagagggtac gtgggcgaga taatagtacg cccgaggacc
      181 cccaacataa tgctccacta ctacaacaag atgcccgaga agaccgtaca ggatttcagg
      241 aacttctggt tccacacggg ggacgcgggc atgatggaca aagacggata catttatttc
      301 gtcgacaggg tgaaggacta catacggagg aggggagaga acataagctc cttcgaggtc
      361 gagaagatag tgagctccca ccccgagatc gaggaatgcg gagtaatcgg catcaaggcc
      421 gagagcggga ggtatgccga ggacgaggtc atgatcgtcg tcgtatgcag gaaggggaag
      481 aagatcgacc cgcacaagct catggagttc ctcgagccca agatgcctca cttcatgctc
      541 ccgaggttca ttcgtttcga aacgacgctg ccgaagacag gcacggagag ggttcagaag
      601 aacaagctca gggaatcggg cataacaaaa gatacctggg acagggaaaa gtccggatac
      661 aaggtcaaac gctgagtctt taccttaagc gctttacgaa taaaatttgc ggctgactgc
      721 ggcgcgatct atagttggta taaatttcaa atgtagaagg agagtgttca tgtctaggat
      781 aatgaaagct tcgctcccgt tcctggcggg cgttctgttc gcagcgatta tcatgggctc
      841 gtttaatatt ttcagggaag cccaggcgca gccgggctac cagcccaaca tggagcaggc
      901 cctcggcagt ctccaccagg cacgcatcag tctcgaaatc tcgtcgcccg acaagggcgg
      961 ccacagggtc agggccctcg agctcgtgaa ccaggcgata gaacaggtca ggctcggcat
     1021 gtatttcgcc gagtgagcag gcctgagacc ctataattca attcagtgct tttggctctg
     1081 ctccatggag tccggatgtg aagagattcc ttcatcgcgg acgtaacagg gactctgcag
     1141 taacttaaga ataaatccga ataggcccac gcgccgtcta aagcgcagag gacaaacggt
     1201 tatgcagccc gttttattcc tcggggggcc atgagacatt taatattggc gttgctgatg
     1261 cctgcggtag cgatgctcgc ctgtgcgaag gcgcagtaca gcccgcccga cctgggcggt
     1321 atatatacgc aggcggccat gcgctcgcac gaagagggca atcccgtcat agtaatcccc
     1381 ggtatcctcg gctcgaagct gagggacagc gaaacaggcc agctcgtctg gggagctttc
     1441 gacggcggcg aagccgatcc ggagaccccg gagggcgcgc gcctgatcgc gataccgatg
     1501 aaggaaggcg tcccgattga cgagctcacg gacagcgtgc gctcggacgg agccctcgac
     1561 agggtcaagg taaagctctt cgggctcccc ctacagctaa acgcttatat caacatactc
     1621 agcacactcg gcgcgggcgg atacctcgac cagtccctcg cgtcaaacaa gctgaacgag
     1681 atcgattacg gcaaagagca cttcacgtgc tttcagttcg actacgactg gaggctcgac
     1741 aacgtcgaga acgcgaagcg gctcaagaaa ttcatcgagg aaaaaaagga atacatactc
     1801 ggggaataca aaaagcgcgg aataaataaa gacgacgtca aattcgacat agtggcccat
     1861 tcgatgggcg gtctcctgac acgttatttt ctcatgtacg gcgacgccgg cctaccccga
     1921 gacggctcaa agccggtggt cacgtgggca ggggcggagt atgtcgaaaa ggtgatcata
     1981 gtcggcacgc ctaacgcggg cgccgtggga gcggtcgaaa ccctagttaa ggggagggac
     2041 ataggcccct tccttcctaa atacgagcct gccataatag cgacgatgcc ctcactctac
     2101 cagcagctcc cgaggacgag acacggcgag gttgttgacg agaacggcca acggctcgac
     2161 ctcatggac
//
LOCUS       DASSJH010000200         5149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_374874, whole genome shotgun sequence.
ACCESSION   DASSJH010000200 DASSJH010000000
VERSION     DASSJH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374874"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /gene="cysC"
                     /locus_tag="VFJ67_04260"
     CDS             <1..477
                     /gene="cysC"
                     /locus_tag="VFJ67_04260"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET7289631.1"
                     /translation="NEVEKKLISTGVRTFILDGDNVRMGLCKDLGFSEADRAENIRRI
                     GEVSKLFVNAGCLVLSAFVSPYRRDRDAVRALVEDGEFVEIYVKAPLEVCEQRDVKGL
                     YKKAREGVIKGFTGIDDPYEEPLNPEITIETDKVSLAEGAEQILGYLSGRHVLNSN"
     gene            522..1463
                     /locus_tag="VFJ67_04265"
     CDS             522..1463
                     /locus_tag="VFJ67_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET7289632.1"
                     /translation="MRKRILVTGGAGFIGSHLSERLLAEGNEVVCVDNFFTGSKENIK
                     HLLQNPYFEVQRHDICFPLYLEVDEIYNLACPASPVHYQFDPVQTTKVNVSGSINMLG
                     LAKRLKIKIFQASTSEVYGDPSEHPQRESYWGNVNPIGPRACYDEGKRCAETLFFDYY
                     RQHGIQIKVLRIFNTYGPRMLPNDGRVVSNFIIQALKGEDITVYGDGSHTRSFCYVDD
                     TVEAIIRMMKSPDGFTGPVNAGNPEEVTILSLARKIIELTGSSSKVVFSGLPEDDPRR
                     RKPDITLAREALGWNPVMGIDKGLEKTIDFFRSRLAI"
     gene            1701..2942
                     /locus_tag="VFJ67_04270"
     CDS             1701..2942
                     /locus_tag="VFJ67_04270"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET7289633.1"
                     /translation="MTAEIAERARKASFSLAKAASGPKNDALLRMAKSITKRAEYIIG
                     ENEKDVEEARAKGLSPAMIDRLRLTEERLDKIADGLRQVAAMEDPVGEIVRTWKRPNG
                     LLVGKMRIPLGVIGIIYESRPNVTADAAGLCVKSGNAVILRGGSETIRSNVAIGEVLR
                     DSLREAGLPEDAVQVIPVTDRDAVLEMLKLEEYIDLIIPRGGEGLIRFVSENSRIPVL
                     KHYKGVCHVFVDESADMGMAERICVNAKVQRPGVCNAMETMLVHEKIAKKFLLAVAKK
                     LEEEGVELRGCEVTRKILPGIKKAEESDWHEEYLDLILSVKVVKDIDEAISHIEKYGS
                     MHTESIVTENYTNSQKFLNEVNSSTVMVNASTRFSDGYELGLGAEIGISTSKLHAFGP
                     MGALELTTTKFVVFGDGQIRE"
     gene            2971..4317
                     /locus_tag="VFJ67_04275"
     CDS             2971..4317
                     /locus_tag="VFJ67_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7289634.1"
                     /translation="MKRARLKSKTLPASERKASRSGPVSKKKDKKKESFTSSEYDNFR
                     DEIEAVPQEEELFIEIDEKVLVELSEAEIDIKEESPEDDESGEEGGKQFTPDEEFRLL
                     QVYFKEMGMEPLLTPKEEIEVSAKIKRCEARAAEMRTLLESVFQKRLGKALQGTVSEV
                     EGLKRSTISARLKSAKRLKTALRKLDGEAAKKSIRRSTLLSKVYLMRARRFKERFIKA
                     NLRLVVSIAKRYMSRGLPLPDLIQEGNVGLMRAVERFDHTKGYKFSTYASWWIHQAIS
                     RSLLDQTRTIRVPVYVLEQATKVHRISSAMNKEMGRKPLPEEISRKTGISVEGVKRVL
                     EATKEVVQLDSPVLDGEKTTLLDFIQDDISGSPDYAVAKATLTSKIKDALSTLTPREE
                     QILKMRFGIGFETTFTLDEIGKHFKLTRERIRQIEKRALEKLEDSDVGAILKSFIE"
     gene            4323..>5149
                     /locus_tag="VFJ67_04280"
     CDS             4323..>5149
                     /locus_tag="VFJ67_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7289635.1"
                     /translation="MLLGLNLRNFTVIDEIAVSFGPGLNIITGETGAGKSVIVDAVNV
                     ILGDRVSPEYIKTGREEAHLEALFDISDKPAVKERLDALGFDAAGGELLIKRIIYRKG
                     RSRAFINGGIATLPVLGQASEGIIDIFSQHEHQSLLRAENHVRVLDEYGGHGALAEAQ
                     RALYARFREATREIERLTGDQRSLYDKEDFLKFQRAEIDDALLREGEDKELEEEKKRL
                     INHERLLTAASGAYESLYENEPSLLGTLSKLSSDLKDAGRIDPSLKEAGDSIENAAI"
BASE COUNT         1340 a         1328 c         1598 g          883 t
ORIGIN      
        1 aacgaggtcg aaaagaagct catttcaacg ggcgtcagga cattcatact ggacggagac
       61 aacgtaagga tggggctctg caaggacctc gggttctccg aggctgacag ggcggagaac
      121 atcaggcgca ttggggaggt atcgaagctg ttcgtcaacg cgggctgctt agtgctgtcg
      181 gcgttcgtca gcccctacag gagggacagg gacgccgtga gggcgctcgt cgaggacggt
      241 gagttcgtcg agatatacgt gaaggccccc ctcgaggtat gcgagcagag ggacgtaaag
      301 gggctctaca agaaagcgag agagggcgtc ataaaggggt ttaccgggat agacgaccca
      361 tacgaggagc cgctcaaccc tgaaataacc attgagacgg acaaggtgag cctcgccgag
      421 ggcgccgagc agatactggg ctacctctca ggcagacacg tgctgaattc gaattagcca
      481 agcaggcggt attacatgcg cgcagtaaag gacggtgcgg aatgaggaag agaatcctcg
      541 tgaccggagg ggcggggttc atagggtccc acctctcgga aaggctcctt gccgagggga
      601 acgaagtcgt gtgcgtcgat aacttcttca cgggatcgaa ggagaatatc aagcacctgc
      661 tccagaaccc ttattttgaa gtgcagagac acgacatttg ttttccgctg tatctcgaag
      721 tggatgagat ctacaacctc gcatgcccgg cctcaccggt acactaccag ttcgatccgg
      781 tacagactac gaaggtgaac gtatcgggct ctatcaatat gctggggctc gcgaagaggc
      841 tcaagataaa gatattccag gcatcgacga gcgaggttta cggggacccc tcggagcacc
      901 cgcagcgcga gagctactgg ggcaacgtga acccgatagg accgcgcgcc tgttacgacg
      961 agggcaagcg gtgcgcagag accctctttt tcgattatta ccggcagcac ggcatccaga
     1021 taaaagtgct gaggatcttc aacacatacg gcccgaggat gctccccaac gacggcaggg
     1081 tcgtgagcaa cttcattata caggcgctca agggggagga cattacagtt tacggcgacg
     1141 gctctcacac caggtctttc tgctacgttg acgataccgt ggaagcgatc ataaggatga
     1201 tgaaaagccc ggacggtttc accgggcctg taaacgcagg gaacccggaa gaggtgacta
     1261 tcctcagcct ggcaagaaag ataatcgagt taacgggctc gtcgtccaaa gtcgtattca
     1321 gcggcctgcc cgaggacgac cccaggagga gaaagcccga cataacgctc gcgcgcgaag
     1381 cgctcgggtg gaaccccgtc atgggcatag ataagggtct cgaaaagacg atcgattttt
     1441 tcaggtcgcg gctggccata tgagcccggc ctgattcgcg tatctgcaac cgttttcgag
     1501 gctttccggc cccgcgccat gccggtgggt ccgggtcagg cggtcagggc ggctcctttc
     1561 ggcgtattca gacaaaaaat agtcttgact ttttgtaacc gactgtatta tcatttaaat
     1621 accgatccta ggactatcaa cttatctaaa tgtctgatat caaagtgaaa aacaaactga
     1681 agcgaacccc gttaacgaat ctgacggcgg aaatcgcgga aagggccagg aaggcttcgt
     1741 tcagtctcgc caaggcggct tccggcccga agaacgacgc cctcctgcgt atggcgaaaa
     1801 gcattacgaa gagggccgaa tatataatcg gagagaacga gaaagacgtc gaggaagcgc
     1861 gggccaaagg gctttcgccc gccatgatag accgtctcag gctaaccgag gaacgcttgg
     1921 acaaaatagc cgacggtctc aggcaggtag cggccatgga agacccggtc ggcgagatcg
     1981 tccgcacgtg gaagcgcccg aacggtctcc tcgttgggaa gatgaggata ccgctcggtg
     2041 tcatcggaat aatttacgaa tcgaggccga acgtaacggc cgacgccgcc gggctctgcg
     2101 tgaagtcggg gaacgcggtc atactcaggg gcggctctga aactataagg tcgaacgtcg
     2161 cgatcggaga ggtgctgaga gactccctca gggaagcggg gctgcccgag gacgcagttc
     2221 aggtcatacc ggttacggac agggacgccg tgctcgagat gctcaagctc gaggaataca
     2281 ttgacctcat cataccgagg ggaggggagg gcctgatcag gttcgtttct gagaactccc
     2341 gcatacctgt gctcaagcat tacaagggag tgtgccacgt tttcgtcgac gagagcgcgg
     2401 atatggggat ggcggagagg atatgcgtca acgccaaggt ccagcgtccg ggcgtgtgca
     2461 acgccatgga gacgatgctc gtccacgaga agatagcgaa gaaattcctc ctggccgtcg
     2521 ccaaaaagct cgaagaggaa ggcgtcgagc tgaggggctg cgaggttacg aggaagatac
     2581 tcccgggtat aaaaaaagcg gaggagtccg actggcacga agagtacctc gatctcattc
     2641 tgagcgtgaa ggtcgtgaaa gacatcgacg aggccataag ccacatagag aaatacggct
     2701 ccatgcacac agaatccata gtcacggaaa attacacgaa ttcgcaaaaa ttcctgaacg
     2761 aggtaaattc atctactgta atggtgaacg cgtcaacgcg tttcagcgac ggttacgagc
     2821 tcgggctcgg tgccgagata ggaataagca cgtcgaagct ccacgccttc ggtcccatgg
     2881 gggcgctgga gctgacaacg acgaagttcg tcgttttcgg agatgggcag atcagagagt
     2941 agtgccatct taattagagg aggatggaaa gtgaagagag ccagactgaa gagcaaaacc
     3001 cttcctgcgt ccgaacgtaa ggcttcgaga agcgggcccg tttcaaaaaa gaaagacaag
     3061 aagaaagaga gtttcacgag ctcggaatac gataatttca gggacgagat tgaagccgtc
     3121 ccccaggaag aggagctctt catcgagata gacgagaagg tcctcgtaga gctgtcggaa
     3181 gccgagatag acatcaagga ggagagcccg gaggacgacg aatccggcga ggaaggcggc
     3241 aagcagttca cgcccgacga ggaattcagg ctcctgcaag tctatttcaa ggagatgggc
     3301 atggagccgc tccttacccc gaaagaagag atagaggtct cggccaagat aaagaggtgc
     3361 gaggcgaggg ccgccgagat gaggacgctt ctcgagagcg tgttccagaa gaggctcggc
     3421 aaggcgctcc agggcacggt ctccgaggtg gagggtctca agcggagcac tatatcggcc
     3481 cgcctcaaga gcgcgaagag gctcaagacc gccctcagga agctcgacgg tgaggcggcg
     3541 aaaaagagca taaggagatc gaccctcctt tcgaaggtct atctgatgcg ggcgcggagg
     3601 ttcaaggagc gttttataaa ggcgaacctg aggctcgtag taagcatcgc caagaggtac
     3661 atgagcaggg ggctcccgct ccccgacctg atacaggaag gcaacgtcgg cctgatgagg
     3721 gcggtcgaga ggttcgacca cacgaaggga tacaagttct cgacatacgc ctcgtggtgg
     3781 atacaccagg cgatctcacg ctcgctcctc gaccagacga ggaccataag ggtccccgtt
     3841 tacgttctcg agcaggcgac caaggtgcac aggattagct ccgcgatgaa caaggagatg
     3901 gggcggaagc ctctccccga ggagatttcg aggaagacag gcatctccgt cgaaggcgtg
     3961 aagcgcgtgc tcgaagcgac gaaagaagtc gtccagctcg actccccggt tctcgacggc
     4021 gaaaaaacga cgctcctcga tttcatccag gacgacatct cgggctcgcc cgattacgct
     4081 gtcgcaaagg ccacgcttac atccaagatc aaggacgccc tttccacgct cacgccgcgc
     4141 gaggagcaga tactcaagat gaggttcggc atagggttcg agaccacttt cactctcgac
     4201 gagatcggga agcacttcaa gctgacgcgg gagcgcataa ggcagataga aaagagggcg
     4261 ctcgaaaaac tcgaagattc cgacgttgga gcgatactaa aaagcttcat cgagtagagc
     4321 cgatgctgct cggactcaat ctcaggaatt tcaccgtcat agacgaaatc gccgtatcct
     4381 tcggccccgg cctcaatatc atcacgggag agaccggagc ggggaagtcc gtcattgtgg
     4441 acgccgtgaa cgtcattctc ggcgacaggg tgtcccccga atacatcaag accggcaggg
     4501 aagaagcaca cctcgaagcg ctcttcgaca tatccgataa acccgctgtc aaagaaaggc
     4561 tcgacgctct tgggttcgac gccgcgggcg gagagctcct cataaagcgc atcatataca
     4621 ggaagggaag gagcagggcc ttcataaacg gcggcatcgc cacgctcccc gtgctcgggc
     4681 aggcgtcgga agggattata gacatattca gccagcacga gcaccagagc ctgctcaggg
     4741 ccgagaacca cgtaagggtg ctcgacgaat acggcgggca cggagcgctc gctgaggcgc
     4801 agagggcgct ttacgcgcgg ttcagagagg cgacgaggga gattgagagg ctcaccggag
     4861 accagaggag cctctacgat aaggaggact tcctcaagtt ccagcgcgcg gagatagacg
     4921 acgcgctcct gagggaaggc gaggacaagg agctcgagga agagaagaag aggctcatca
     4981 accacgaacg cctcctcacg gcggcgagcg gtgcctacga gtctctttac gaaaacgagc
     5041 cctcgctact ggggacgctc agcaaactgt cgtccgatct caaggacgcg ggcagaatag
     5101 acccgtcgtt aaaggaagcg ggggactcga tagaaaatgc ggccattca
//
LOCUS       DASSJH010000201         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1020532, whole genome shotgun sequence.
ACCESSION   DASSJH010000201 DASSJH010000000
VERSION     DASSJH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020532"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFJ67_04285"
     CDS             <1..834
                     /locus_tag="VFJ67_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET7289636.1"
                     /translation="ITDFGVFVELEPGIEGLVHISEITWTKKFRHPKEIIQAGSAVEA
                     VVLDVDSENRRISLSLKQIEPSPWQLFKEKNPPNSRVKGVIKNVTDKGLFVEVEENIV
                     GLVRPDNISWKGRVNPEESFERGKEIEVVVLNVDDRNQRIGLGLKQLSADPWEEVQKK
                     YRAGQSSATGKVKEVKDNGIVVELEEGIEGYIKANELSQERGQREITKSFSVGDEITA
                     QVTGFDKKKRQVFLSKRKYDEWQDKERVSNFISSQGEPTVKLGDVLKEKLKQMNNDEV
                     I"
     gene            837..1718
                     /gene="sppA"
                     /locus_tag="VFJ67_04290"
     CDS             837..1718
                     /gene="sppA"
                     /locus_tag="VFJ67_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7289637.1"
                     /translation="MKSAFVVIGVLVLLFFAFLAGMGCGLLISGDDGFSAGDRVAVLR
                     IDDVIVDDQAYIDSLDKIKEDKKVKALVVRIDSPGGAVGPSQEIYSELKKLRSDMPVI
                     ASIGNVGASGGYYIACAAEKIFANPGSITGSIGVVAEFMSYEKLLEWARVDVEVLKSG
                     KYKDVGSPFREMTPEDREYMQGLIDNVYSQFKSAVAESRGIGAAEVDKIADGKIFTGE
                     QAKRLKLVDDMGTISDAVNLAAQKAGIKGKPDLVYYPKKKSQLLELLDSKLDIPALTG
                     RPSKFSFGLFYLVDIIN"
     gene            1721..2206
                     /locus_tag="VFJ67_04295"
     CDS             1721..2206
                     /locus_tag="VFJ67_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014121409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET7289638.1"
                     /translation="MKVLWAPWRIQYITGDKEEGCIFCRKPKEGNDKKNLILYTGETS
                     FIIMNRYPYSNGHLMAVPYKHTNNLSDLTQEERLELMNLTVKCTDILKAIRPDGFNIG
                     MNLGRAGGAGIDDHLHYHIVPRWNGDSNFMAVIADVRVMPEYLEETYETLSKHLKSLE
                     R"
     gene            2211..2600
                     /locus_tag="VFJ67_04300"
     CDS             2211..2600
                     /locus_tag="VFJ67_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289639.1"
                     /translation="MRYIKVLFIILIILFFAILYTQNMEMFTHEITLGLNLKFYKIGP
                     YITMNVVIILTAFAAGAVVAVIFGALQSIGAGAESKEKSRRIRELETRNRELIEEIQR
                     EEKLKKSDPSSSPFSPPSAIDSSSQNS"
BASE COUNT          779 a          586 c          789 g          509 t
ORIGIN      
        1 ataacggatt tcggggtctt cgtcgagctc gagcccggca tcgaagggct cgttcatata
       61 tcggaaatca cctggacaaa aaagttccgt catccgaaag agatcataca ggcagggagc
      121 gcggtcgaag ccgtcgtgct cgacgtggac agcgagaaca ggcgcatatc gcttagcctg
      181 aaacagatcg agcccagccc atggcagctc ttcaaggaga aaaacccgcc taattcgagg
      241 gtaaagggag tgatcaagaa cgttacggat aagggtttat tcgtcgaggt tgaagagaat
      301 atcgtcggac tcgtgagacc ggacaacata tcctggaaag gcagggtaaa cccggaggag
      361 tccttcgaga gaggaaagga aatagaggta gtcgtgctca acgtggacga caggaaccag
      421 aggatagggc tcggcctgaa acagctttca gccgacccgt gggaagaggt gcagaaaaaa
      481 tacagggccg gtcagtcgtc cgcaacggga aaggtcaagg aagtgaaaga caacgggatc
      541 gtggtcgagc tcgaagaagg gatagagggt tacatcaagg cgaacgagct gagccaggaa
      601 agagggcaga gggaaatcac caagagcttc agcgtagggg acgagatcac ggcgcaggtg
      661 accgggttcg acaagaagaa gaggcaggta tttctcagca agagaaagta tgacgaatgg
      721 caggacaagg aaagggtctc gaacttcatt tcgtctcagg gagagccgac ggtcaagctt
      781 ggagatgtgc tcaaggaaaa attaaagcaa atgaataacg acgaggttat ctgagggtga
      841 agagcgcttt tgtggtgatc ggggtactgg ttcttctctt cttcgccttc ctggccggta
      901 tgggctgcgg tctcctgatt tcgggcgatg acggtttctc ggccggggac agggtcgcgg
      961 tgctgaggat cgatgacgta atagtcgacg accaggctta tatagacagc ctggacaaga
     1021 taaaagaaga caagaaggta aaagcgctcg tcgtgaggat cgactcgccc gggggcgctg
     1081 tcggcccctc acaggagatc tacagcgagc tcaaaaaact gaggagcgac atgccggtca
     1141 tcgcgagcat cgggaacgtc ggagcctcag ggggttatta catcgcgtgc gcggctgaga
     1201 agatattcgc caacccgggc tcgatcacgg ggagcattgg agtcgtcgcg gagttcatga
     1261 gctatgaaaa gctcctcgaa tgggccaggg tggacgtgga agtgttaaag agcggaaagt
     1321 ataaagacgt gggctccccg ttcagggaaa tgactcccga agacagggaa tacatgcagg
     1381 gactgattga caacgtatat tcgcagttca agtcggcggt cgccgagtcg aggggaatcg
     1441 gggcggctga agtcgacaaa atcgcggacg ggaagatttt cacgggggag caggcgaaac
     1501 ggctaaagct ggttgacgac atggggacaa taagtgacgc cgtcaatctc gcagcccaaa
     1561 aggcgggaat aaaaggaaag ccagaccttg tttattatcc gaaaaagaaa tcccagttac
     1621 tcgagctcct tgattccaag ctggacatac cggcccttac cggcaggccc tccaaattca
     1681 gctttggact tttctatctg gttgatataa ttaactgata atgaaagtgc tctgggcgcc
     1741 ctggaggatc cagtacataa cgggggacaa ggaagaggga tgtatttttt gcagaaagcc
     1801 caaagaaggg aatgacaaaa agaacctcat attatatact ggtgagacta gcttcattat
     1861 aatgaaccgt tatccatatt cgaacggaca ccttatggca gtcccttaca aacacacgaa
     1921 taatctctcc gacctgacgc aggaagaaag gctcgaactg atgaacctta cggtcaaatg
     1981 cacggacatt ctgaaggcga taagaccgga cgggtttaat ataggtatga acctgggcag
     2041 ggccggcggg gccggtatcg acgaccacct ccactaccac atagtgccga ggtggaacgg
     2101 ggacagcaat ttcatggccg tgatcgcgga cgtgagggta atgcccgaat acctcgaaga
     2161 gacgtacgag accctaagca agcatttaaa atcattggag aggtgacgat atgagatata
     2221 taaaagttct gttcataata ctgataatac tctttttcgc aatcctgtat acacagaaca
     2281 tggaaatgtt tacgcacgag atcacgctag ggctcaacct caagttttac aaaatagggc
     2341 catatataac tatgaacgtc gtaataatcc tgaccgcgtt tgcggcggga gcggtcgtag
     2401 ccgtaatttt cggcgcgctc cagtcgatag gcgcgggagc ggaatcgaaa gaaaaatcga
     2461 gacgcataag agagctcgaa acgcggaaca gggagctcat agaggaaata cagagggaag
     2521 agaagctgaa aaaaagcgat ccttcttcat ctccgttcag ccctccgtcc gcaatcgaca
     2581 gctcctctca aaacagctga cggggccttg gtgccgcagt cacgagggat tcaaatatta
     2641 atatgaggaa cagaggaaaa ccc
//
LOCUS       DASSJH010000202         4495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1128652, whole genome shotgun sequence.
ACCESSION   DASSJH010000202 DASSJH010000000
VERSION     DASSJH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128652"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1643
                     /locus_tag="VFJ67_04305"
     CDS             138..1643
                     /locus_tag="VFJ67_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7289640.1"
                     /translation="MKICTITFLASLLIFFTLAADPSSSAEDELEGLDAFIELAMEQY
                     DVPGVSVAVVKDGEIVYLKGFGVRTISTKDKVDGDTVFQLASVTKTFTAASVASMVDR
                     GKLGFDEEVIKVIPDFALMDPYSTRYTTARDLLAHRTGLPAFTGDIFDNLGYSREEVI
                     RRVRYFTPACSFREEANYSNIGYFLAGQEAAYAADSSWEDVVRENIIKPLGLKHTGFT
                     YEMDKEKNLASPHAVVDGETKVIPPNQQRVLAPAGAMTSSASDLARYLIMHLDQGKFR
                     GKTILSQESVEQLFTPAIVDKPTFAELPPITGDNGFSYGLAWGIYYWHNHKILEKGGA
                     LDGYRSVVVLVPDLKLGIAVLANMNLTVFPEAVRAFVLEQYLGEADYDMQEAIMERSK
                     KMGEMIGLDRTVKPENPRPPSKDLDAYTGAYENDLYGSFSVIRDGDNLKVEAGPAKYT
                     GTLTHVNYDTFYLKWPVFISSPDEVTFVIDAEGNVTEYIDSALGRFKKVKE"
     gene            complement(1826..2083)
                     /locus_tag="VFJ67_04310"
     CDS             complement(1826..2083)
                     /locus_tag="VFJ67_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289641.1"
                     /translation="MTKKYLHIMLVAGALALGACAGQNQQAQEDDGFDMSICNNIPVS
                     VEAYEAMDPADKQNKISCADLERCASYMGNDTPPNVPCEGP"
     gene            2248..2589
                     /locus_tag="VFJ67_04315"
     CDS             2248..2589
                     /locus_tag="VFJ67_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289642.1"
                     /translation="MSGQPPLAAVDNYYYYLNMITENVRNGYDIMVLKYCGLSLPLIP
                     ALIEVTKQDSGEFDVSTIPAIEIGARLWSQQGQKDKIDELSRLVSAYPELSPWKIHID
                     RAYDTLRELEK"
     gene            2679..3500
                     /locus_tag="VFJ67_04320"
     CDS             2679..3500
                     /locus_tag="VFJ67_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289643.1"
                     /translation="MSKRLIVTILAAAFTAALAYTFTPYARESGPAEAADSSKEKPAD
                     NPGPAAAPVKELTLDEILGKYYKAIGGLADWGTLNTLAVKGKIVSQNAEIATTAEYMR
                     PDKCRLTYKIGGKLVVQSFNGRTAWQQSAMSESVAPELLDAGRTAYLRDRCAIESPLI
                     DYAKKNLRVTLEGRDIVDGKDAYKIKVTYASGNFQYYLLNSASFLPVKAVGFYTVDGK
                     EVVMMTRFRDYTRVGSLLIPFKLEIEKKGNAPREEYIVESVIPNPPIDPAIFNMP"
     gene            4173..>4495
                     /locus_tag="VFJ67_04325"
     CDS             4173..>4495
                     /locus_tag="VFJ67_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289644.1"
                     /translation="MKNKNELILPALVLGLLVAVAAWIPINYTGDLFYYKKLRDEGVE
                     TTAEVMMKAIMTDGRLVRTNITSPSDDHRIMVSLRLPRGVTSVCTLHVSKSTYDVVSK
                     RDTLGV"
BASE COUNT         1051 a         1411 c         1122 g          911 t
ORIGIN      
        1 cgaaccgttc ctcgtaaagt tttaacccat cctcacacag cgccccccct cccctccgcc
       61 ccgcgagtgt tattgtctcc cctcccgttt catgttacgt tgaaagtacc gcttcaacat
      121 ccggccggag gtccctgatg aagatttgca cgatcacctt tttggcgtcc ctgttaattt
      181 tcttcacact cgcagcagac ccctcctcat ccgcggagga cgagctcgaa gggctcgacg
      241 ccttcataga gctcgccatg gagcagtacg atgtccccgg cgtttccgtg gccgtcgtga
      301 aggacggcga gatcgtttac ttgaagggtt tcggcgtaag gaccatcagc actaaagata
      361 aagtggacgg cgacacggtg ttccagctcg cctccgtaac gaagacgttc acggccgcct
      421 cggtcgcctc catggtggac aggggcaagc tcggcttcga cgaggaagtg ataaaagtaa
      481 tcccagactt cgccctcatg gacccctatt cgacccgcta cacaaccgcc cgcgacctgc
      541 tcgcccaccg caccgggctc cccgcgttca cgggcgacat attcgacaac ctcggctact
      601 ccagagagga agtcataagg cgggtgcgct acttcacccc cgcatgcagc ttcagggagg
      661 aagcgaacta ctccaacatc gggtacttcc tcgcgggaca ggaagctgcc tacgcggccg
      721 attcctcgtg ggaagacgtc gtcagggaaa acataatcaa gccgctcggc ctcaagcaca
      781 cgggcttcac atacgagatg gacaaggaaa agaacctggc ctcccctcac gcggttgtgg
      841 acggcgagac gaaggtcata ccgccgaacc agcagagagt gctcgccccg gcgggcgcga
      901 tgacgtcctc cgcgtccgac ctcgcccgat accttatcat gcacctcgat cagggcaagt
      961 tcaggggcaa gactattcta tcgcaggaat cggtcgagca gttattcacg cccgcgatag
     1021 tcgacaagcc tacgttcgcg gaattgcccc ccatcaccgg cgacaacgga ttcagctacg
     1081 gactcgcctg gggcatctac tactggcaca accacaagat tctcgagaag ggtggcgcgc
     1141 tcgacggtta caggagcgtc gtcgtgctcg tgccggacct gaaactcggc attgcggtgc
     1201 tcgccaacat gaacctcacg gtcttccccg aggccgttcg cgcgttcgtt ctcgagcagt
     1261 acctgggcga agccgactac gacatgcagg aagcgataat ggaaaggtcg aagaagatgg
     1321 gagagatgat cggcctcgac aggacggtca agcccgaaaa cccgcgcccc ccgagcaaag
     1381 acctcgacgc gtacaccggg gcgtacgaga acgacctcta cggcagcttt agtgtcatca
     1441 gggacggaga caacctcaag gtcgaagcgg gcccggccaa gtacacgggc acactcacgc
     1501 acgtcaatta cgacacgttc tacctcaaat ggcccgtatt catttcatcg cccgacgagg
     1561 tcacgttcgt aatcgacgcg gaaggcaacg tcacggaata catcgactcc gccctcggca
     1621 ggttcaaaaa ggtgaaggaa tagcttcaac agcacatcgt ttttcgcagt tgcttccctc
     1681 atttgtagga gcggcttcca gccgcgatac gaatccgttc gcccttaatt taatttccct
     1741 ttcccttgag gggggagggt ttgggtgggg gtgttacctt tcattcaata agccagtcat
     1801 actgaaccaa tcctgtaaca gattatcaag gcccctcgca aggcacgttc ggcggcgtat
     1861 cgttacccat gtacgacgcg cacctctcga gatcggcgca gctgatcttg ttctgcttgt
     1921 ccgccgggtc catggcctcg tacgcctcca cgctcacggg aatattattg catatgctca
     1981 tatcgaaccc gtcgtcttcc tgtgcctgct gattctgacc cgcgcacgcc ccgagagcga
     2041 gtgcccccgc cacgagcatt atatgtagat acttcttagt catgagtctc tatcctcctg
     2101 gttacttacg aaattctccc tctcattata cacccgatag agccaaaaaa tagccctgcg
     2161 cggcccgctc ggaactcacg gcggacctgc tgccattgcc tttccgccgc ctccgattat
     2221 atttttctcc ggctgaagga gcacgagatg agcggtcaac cccccctcgc ggcggtcgat
     2281 aactactatt actacctgaa catgatcacg gagaacgtgc ggaacggcta cgacatcatg
     2341 gtgctgaaat actgcggtct ctcgctcccc ctcatccccg cgctcatcga agtcacgaaa
     2401 caggactcgg gggaattcga cgtctccacg atccccgcga tagagatcgg agcgagacta
     2461 tggagccagc agggacagaa ggacaagata gacgagctct cacgcctcgt ttccgcatat
     2521 cccgagctct ccccctggaa gatacacatc gaccgcgcct acgacaccct ccgcgagctg
     2581 gagaaataag acttgcgcat ggccctcacc ctccactctt tcccctttga atttccccct
     2641 caatccatta tccttcttcg ttgcaccggg tggcgtacat gagcaaaaga cttatcgtaa
     2701 ccatactcgc agccgcgttt acggcggcac tggcgtacac tttcacgcca tacgcccgtg
     2761 agagcggccc cgccgaagca gcggacagct caaaggaaaa acccgccgac aaccccggac
     2821 ccgcggccgc tccggtaaag gagctaaccc tcgacgagat actcggaaaa tattacaaag
     2881 ccataggcgg cctcgcggac tgggggaccc tcaacacgct cgccgtgaag ggcaagatag
     2941 tttcacagaa cgccgagatc gcgaccaccg ccgagtacat gcgccccgac aagtgcaggc
     3001 tcacgtacaa aataggcgga aagctcgtcg tccagtcctt taacggccgg accgcatggc
     3061 agcagagcgc catgtccgaa agcgtcgcgc ccgagctcct cgacgccgga cgcaccgcct
     3121 acctccgcga caggtgcgcc atcgaaagcc ctctcatcga ctacgcgaag aaaaatctca
     3181 gggtcacgct cgaaggccgc gacatagtgg acgggaagga cgcgtacaag atcaaggtca
     3241 catacgcttc gggcaacttc cagtactacc tcctgaactc agcgtcattc ctgccggtga
     3301 aggcagtcgg cttctacacc gtcgacggga aggaagtagt catgatgaca aggttcaggg
     3361 actacacgag agtcggcagt ctcctcatcc ccttcaagct cgagatagaa aagaaaggca
     3421 acgccccccg cgaggaatac atagtcgaat ccgtcatacc caacccgccc atcgaccccg
     3481 cgatcttcaa catgccctga cgcacacacg ccccagaggc tttgagtcga agcatgtcat
     3541 atcgagcaac agttctgagc aagcgcaaga actggtcgtt ctttgctaat ttcaagtaac
     3601 tcatgttgac atcgtataag acttgcacaa tacaaagcga gaaatccgtt tttgtctttc
     3661 acgacattcc cagattccac tctgagcgaa cacccaggag catctcaact cgcttcagca
     3721 cctgttttcc cgacattaca gaatttgcgt taagaaaata gagtattaag gcgtgaagat
     3781 cgttcagggg acgattccga gctagtgagg aatcggtcgc tctttcttct ccgtaacaac
     3841 gtagattaat tcggcacgtc aaatcttcat cgctccttat gcgggttgaa ttgaacgatc
     3901 ttagccttaa tgcgagattt ttagcaaatt atgttctgtc ggttgaattt tgcttctttt
     3961 catcagggaa aagaaggata aaaaaatatc ttcctcattt ctatgctttt caccggtatt
     4021 cacagaaccg ttgcgtggca aacacacccg gacgaccgta cctgcccccc tttccatctc
     4081 accccgaaag ggatgtatta ttaccccgta cacacggctc gcacccggcc gctccgccgg
     4141 gctcgctcta aaccgccagc atcgtcgtca ttatgaagaa taagaacgaa ctcatcttac
     4201 ccgccctcgt cctcggcctc ctcgtcgccg tggccgcttg gattcctatc aattacacgg
     4261 gcgacctctt ctactataaa aagctccgcg acgaaggagt cgagactacg gccgaggtca
     4321 tgatgaaggc gatcatgacg gacggccgcc tggtgaggac gaatatcacg tccccttcgg
     4381 acgaccaccg catcatggtc tcgctgcgcc tgcccagggg cgtcacgtcc gtgtgcaccc
     4441 tccacgtctc gaaatccacg tacgatgtag tctccaaacg ggacacactg ggcgt
//
LOCUS       DASSJH010000203         4772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1237006, whole genome shotgun sequence.
ACCESSION   DASSJH010000203 DASSJH010000000
VERSION     DASSJH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237006"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFJ67_04330"
     CDS             <1..1039
                     /locus_tag="VFJ67_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HET7289645.1"
                     /translation="ASRPEEEAIYLYSTGSTGKPKRVSRTHFNLAALADNHTQTVGWS
                     CEDRVLFAVPISHTYAFGNYISAVRSGAAVVVTSEFNRQKVLDLLEKEKITVFPAVPV
                     MLDVLSRTHMDAPIDLSSLKLVISAGAPLQEHTFFKFRERFSVYPRQLYGSTETGVIS
                     INLCGDDEIEKRFNSVGRPVRNVTVKVFDEEGREAEAGVEGEIAVKSPSMTTGYYGLP
                     EETARVFRNGYYFTGDLGFIDEAGYIYIRGRKKFLINVGGFKVDPEEVENLLSKHPDV
                     VEAAVLGITDNLGNERVKAVIVTRRQMRVKEVLGFLRVRIAEYKLPSLVEFRTELPRS
                     PAGKILRERLK"
     gene            1061..1330
                     /locus_tag="VFJ67_04335"
     CDS             1061..1330
                     /locus_tag="VFJ67_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7289646.1"
                     /translation="MNNDIKLRVKKVLSRNILRDSDTSDLGDDTPLIEYGVGVDSVSR
                     LEFLVALEEEFGIRLDESEITPEFFETVSGIAGFISRRIELNDGV"
     gene            complement(1327..2043)
                     /locus_tag="VFJ67_04340"
     CDS             complement(1327..2043)
                     /locus_tag="VFJ67_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289647.1"
                     /translation="MAEISLEEIIREAKKRKIDLGADPEKTVKVCSKLGLIPKPKRKR
                     VTEGGATRTRLLFPEDTIDKLAHIKAMKSGGLTLGEIRDSFALEYVQGALKDLLNNAD
                     DEKVKELANIISREKELESIVEAPLVYLIEGMSPDEAKKLLTLFIGVGYYALLEAQKS
                     LEEFKFNDARKSLFRAIFYNSIAVLRLARTTNDTKLEETAFELYEKTVIEPIGRASER
                     VRKEFINSFERYLKEKGLTN"
     gene            2249..3010
                     /locus_tag="VFJ67_04345"
     CDS             2249..3010
                     /locus_tag="VFJ67_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289648.1"
                     /translation="MAKSTLVLYEPLGSLTEKSYYWDEYVQFYEQYWGGPTSPFGVWF
                     GNEYESFVNALSENLGMAKDEIEHCFFTKDEEGKYYIAPYKSKANLFFCENFIPFEWF
                     VPFSEEDRQTLYTHWGFNALHYDTRVHIALGRLDEAEKIVADAINQQSQPVDASVRTY
                     LSEHLSKGLAELKAWLSGFDPGGFLVLNYGELCSVINPYSLENEHSVGETWKILGLFK
                     EGKYTEAESSLGAFMRKWDDVREYASGSLDGSTLQ"
     gene            3364..4599
                     /locus_tag="VFJ67_04350"
     CDS             3364..4599
                     /locus_tag="VFJ67_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7289649.1"
                     /translation="MFKKSEFADVFNDFALGHTESIAESFDQDELDDVDAFFDDEAQL
                     TEEPEQTITEKTSGDEEISQDLRLVNAYFKEVSTEALLAPHEEAHVAAKIKCCEDRAR
                     AVRRKIEKVLGKRVGEEASGVHNSVRNYMETANNGLSHKNGTKHRTASADLLLLARLY
                     EAYVTKGFQLRNRFIKANLRLVASMAKRYVGRGVPFLDLIQEGNLGLIKAVERFDHTK
                     GYRFSTYACWWINQAMTRGIFNQTRTVKMPAYLLEKAGKVRHVRRLLTKDYEREPYAE
                     EIAEKAQMSVENVKRILESSVNKVVRLDTPVWNGEKATYMDFMEDSSSLDVDSVIAEV
                     SIPDNINDALLMLSIREREVVKMRFGIGYDCPYTLDEIGRKFRLTRERIRQIEKKALD
                     RIRRSKSAPVLRSLMEVYN"
BASE COUNT         1152 a         1231 c         1322 g         1067 t
ORIGIN      
        1 ggcctcccga ccggaggagg aggcaatata cctttactcg accgggtcca cgggaaaacc
       61 caagagagta tccaggacac acttcaacct cgcggccctg gcggacaatc acacgcagac
      121 agtaggctgg tcctgcgagg acagggtcct cttcgcagtc cccatctcgc atacatacgc
      181 gttcgggaac tatataagcg ccgtcaggtc aggcgcagcg gtcgtcgtga cctcagagtt
      241 caacaggcag aaggtgctcg acctcctcga aaaggagaaa atcacggttt tcccggccgt
      301 accggtcatg ctcgacgtgc tgtccaggac ccacatggat gcgccaatcg atctctcttc
      361 actgaaactc gtcatatccg cgggcgctcc tttgcaggag cacacgtttt tcaaattccg
      421 cgagaggttt tccgtctacc cgcgccagct ctacggatcg acagagacgg gggtcatatc
      481 cattaacctc tgcggcgacg atgaaataga gaagcggttt aactcggtcg gtagacccgt
      541 gaggaacgta accgtgaagg tgttcgatga agagggccgc gaggccgagg cgggcgtaga
      601 gggagagatt gcggtcaaaa gcccgtccat gacgaccggc tactacggac tccccgaaga
      661 gacggcgcgc gtattcagaa acggatatta ctttactggc gatctgggtt tcatcgacga
      721 ggctggctat atatacatca gggggcgcaa aaaattcctc atcaacgtcg gcggtttcaa
      781 ggtcgatccc gaggaagtgg agaatctctt atcgaaacac ccggacgtcg tcgaggccgc
      841 cgtattgggc ataacggata acctcggtaa cgagcgggtg aaagctgtta tcgtcacgcg
      901 acggcagatg cgcgtaaagg aagtactcgg cttcttgagg gtgaggatag ccgagtacaa
      961 gctcccctca ctggtcgagt tccgtaccga gcttccgagg agcccggctg gtaaaatact
     1021 tagggagcga ttaaagtgag agccttacgg gaaacgtcgt atgaacaatg atatcaagtt
     1081 aagggtgaag aaagtgctct ctagaaatat cctcagggat tcggacacga gcgatctggg
     1141 cgacgatacc ccgctgatag aatacggcgt cggcgtcgac tccgtttcga ggctcgagtt
     1201 cctagtcgcg ctcgaggagg agttcggcat aaggctcgac gaaagcgaga taacacccga
     1261 attttttgaa accgtatccg gtattgccgg ttttatatcc cgcaggatag agctaaatga
     1321 cggcgtctag ttggtcagtc ccttttcctt gagatacctc tcgaacgagt ttatgaattc
     1381 cttccttacc cgctcgctcg cccgccctat gggttctatc acggtttttt cgtagagctc
     1441 gaaggcggtc tcttcgagct tcgtgtcgtt cgtcgtcctg gctaacctca gcacggcgat
     1501 agagttatag aatatggccc tgaacagcga cttccgggcg tcgttgaact tgaactcctc
     1561 gagcgacttc tgggcttcga gtagcgcgta gtaccccact ccgataaaaa gagtgaggag
     1621 ctttttggcc tcgtctggcg acatcccttc tatgaggtat acgagcgggg cctccacgat
     1681 tgattcgagc tccttctccc tgcttataat attggcgagc tccttcacct tctcgtcgtc
     1741 ggcgttgttc aggaggtctt taagcgcccc ctggacgtac tctaacgcga agctgtccct
     1801 gatctccccg agcgtgagcc cgcccgactt catggccttt atgtgagcga gcttgtctat
     1861 tgtgtcttcg gggaatagca gccgggtcct ggtcgcgcct ccctcggtga ccctcttccg
     1921 tttgggtttc ggtatgagcc ccagcttcga gcacaccttg accgtctttt cggggtcggc
     1981 gcccaggtct atttttcttt ttttcgcttc tcttataatt tcttcaagtg aaatttcagc
     2041 cataccatga tattaagcgt tatatgaagc ttttgtcaat cggtttttta ttgactttcg
     2101 gtcagttatg ccggtatttc ccctcccgcg gcccgtcagg ccccataaat aactctctct
     2161 aacgccgccc gggtgaaacg atttatgtcc ccgtcgccgg gtctcccctc cgattgattt
     2221 tgagcgggat ttggtttatt tatttcatat ggcgaagagc acgctcgtcc tctacgagcc
     2281 cctcggaagt cttactgaaa aaagctatta ctgggacgaa tacgtgcagt tctacgagca
     2341 gtactggggc ggtcccacgt cccctttcgg agtgtggttc gggaatgagt acgaatcatt
     2401 cgtaaacgcc ctttccgaga acctcggaat ggccaaagac gaaatcgagc actgcttctt
     2461 tacgaaggac gaggagggga agtattacat cgccccctac aagtcgaaag cgaatctgtt
     2521 tttctgcgag aacttcatac ccttcgagtg gttcgtccct ttcagcgagg aagacagaca
     2581 gactctctac acgcactggg gcttcaacgc gctccattac gacacccgcg tacacatagc
     2641 cctcgggaga ctcgacgaag ccgaaaagat agttgccgat gcaattaatc agcaatctca
     2701 gcccgtggac gcctccgtgc ggacgtatct ctccgagcat ttgtcaaaag gtctggcgga
     2761 gcttaaagca tggctctccg gtttcgatcc cggcggcttt ctggtactca attacggcga
     2821 gctctgctcc gtaataaacc cctattccct cgagaacgag cactccgtcg gtgagacctg
     2881 gaaaatcctg ggcctgttta aagaaggaaa atacacggaa gcggaatcct ctctcggcgc
     2941 cttcatgcgg aagtgggacg acgtaaggga atacgcatcc ggaagcctcg acggttccac
     3001 cctccagtga acatgtgatg acccggcacg gaacggaccc gttcccggat taaatcggcc
     3061 ggcggcgtac gtcttaacaa atccccggca tgtgtatgcg gtaatcccct cccgaatgtt
     3121 ttcggtcagc ccctgcgtat aaataattat gaacggggtt cggcgaaaaa ttgcgaaatc
     3181 tccgggttcc ggcttgcgcg ggacttattt caaggagttt cttcaatgat ccttctgact
     3241 tatagtatgc gaattgaatc tgaaaccaat tgccgttcgt aatggtttat agatacaaga
     3301 acgactgaat cgggatgcat ccggcagccg gcctgaagca tcttaaagtg aaggaggcgg
     3361 taaatgttca agaagtctga atttgcggat gtattcaatg atttcgcgct tggccatacg
     3421 gagtctatcg ccgagagttt cgatcaggac gagctcgatg acgtggatgc atttttcgac
     3481 gatgaagctc agctcacaga ggaacccgag cagactatta cggaaaaaac gagcggggac
     3541 gaggagataa gtcaggattt aagacttgta aacgcatatt tcaaagaggt gagcactgag
     3601 gccctcctcg cgccccacga agaggctcac gtcgcggcca agataaagtg ctgcgaggac
     3661 agggcgaggg ctgtgcgcag gaagatagaa aaggttctgg gcaagagagt cggcgaggaa
     3721 gcgtccggtg ttcataacag cgtaagaaat tacatggaga cggctaacaa cggcctctct
     3781 cataaaaacg gaacgaaaca tagaacggcc tcggcggacc tcctccttct cgcgaggctg
     3841 tatgaggcat acgtgacgaa ggggtttcag ctcaggaaca ggtttattaa agcaaacctc
     3901 aggctcgtgg cgagcatggc gaagagatat gtcggcaggg gcgtcccttt tctcgacctc
     3961 attcaggagg ggaacctcgg tcttatcaaa gccgtcgagc ggttcgacca caccaagggc
     4021 tacaggttct ccacatacgc ctgctggtgg ataaatcagg ccatgacgag aggcatattc
     4081 aaccagacca ggaccgtaaa gatgccggct tatctgctcg aaaaggcggg caaggtgaga
     4141 cacgtcagga ggctcctgac gaaggattac gagagggagc cttatgccga ggagatagcc
     4201 gagaaagcgc agatgagcgt ggagaacgtt aagaggatac tggagtcgag cgtgaacaag
     4261 gtggtaaggc tcgacacgcc cgtatggaac ggcgagaagg cgacgtacat ggacttcatg
     4321 gaagactcga gctctctcga tgtcgacagc gtgatagcgg aggtctccat acccgataac
     4381 atcaacgacg ccttactgat gctatcgatc agggagaggg aggtcgtgaa gatgaggttc
     4441 gggatcggtt acgactgccc ctatacgctc gacgagatag gcaggaagtt ccgcctcacc
     4501 agagaaagga taagacagat cgaaaaaaag gcgctcgaca ggataaggag gtcgaaatca
     4561 gcccctgtcc tgaggagcct gatggaagtc tataactaaa aatcaagcgg actgcgtaaa
     4621 cgcaggaaaa tccggccata aaaattgaag aatggtcgat cgacgagggt gcgcccgtga
     4681 taacggtgcg cacccttttt ttatcggctc gcgcccggag cccctctcga gtcgtgtata
     4741 ttatcggatt ggtcgcttca gatgaaaccc gg
//
LOCUS       DASSJH010000204         6989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1020994, whole genome shotgun sequence.
ACCESSION   DASSJH010000204 DASSJH010000000
VERSION     DASSJH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020994"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..635
                     /locus_tag="VFJ67_04355"
     CDS             111..635
                     /locus_tag="VFJ67_04355"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HET7289650.1"
                     /translation="MPGKRVKLLIGRKEKVDFPTLRLYGIDAKVDTGAYTSAIHCDSI
                     RAVRKGGERFVRFRLLDPSHPAYDGREIRKPLLGRKQVKNSFGQTEFRYIVKTRIVIF
                     GKSHDIELSLCDRSGMEHPILLGRKFIQDKFVVDVSRFDLSAKNRVIRIGSNGKPKGR
                     KPGARGGTEPDSRK"
     gene            complement(665..865)
                     /locus_tag="VFJ67_04360"
     CDS             complement(665..865)
                     /locus_tag="VFJ67_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289651.1"
                     /translation="MFRKGLQVILNQPIEGKKDRFEQGLKGMIVEEFDLPHGKSYRVQ
                     FVDGRVARFPEELMKQAVDVIA"
     gene            1096..1791
                     /locus_tag="VFJ67_04365"
     CDS             1096..1791
                     /locus_tag="VFJ67_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289652.1"
                     /translation="MAEFLDKLKVRLDKGLETVNVKSKEIIGRQKIRLHLSELEAERK
                     MVFQDLGKLAYKQYQLSGDAPGFAAGQAPLTITSPGLDKWEESVLDVLGRISDGQLNG
                     ILFEDTPKQFKVTAKTLYKEVEKLIPDSEKKGQNLQWLGRVLGKFRLAAKKVSKRIDG
                     ERETVYVFDKQKTRSALESVKSGAEKSEKPAKETASPSDTDAILVSCKKLADIDSRIE
                     KLKKQMKKIGEKA"
     gene            complement(1822..2151)
                     /gene="trxA"
                     /locus_tag="VFJ67_04370"
     CDS             complement(1822..2151)
                     /gene="trxA"
                     /locus_tag="VFJ67_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7289653.1"
                     /translation="MAANGIIELSDTNFDAEVMKSDVPVLVDFWAPWCGPCRAIAPAV
                     EEISSSYQGRIKVGKLNVDENQQTTMKFGIRSIPTLIVFKGGKAVEQIIGAVPKGEIE
                     RVVGKAL"
     gene            complement(2232..3776)
                     /locus_tag="VFJ67_04375"
     CDS             complement(2232..3776)
                     /locus_tag="VFJ67_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7289654.1"
                     /translation="MTKTLLLILISMTGTGGVMDLSLNDAVRIALENNRDIQIEEKNV
                     AVSEGEIKTQEGVFDPLFNIVSFFNDGEEPVLSTFVPSGTVTQKQFNAEANINGLLPS
                     GTFYNLIDFNTTWTKTTNPLEDLSPSWFNNVGFSLGQELLRNFGVDVNRTFIITATRT
                     NEITVKELEVRISQVLLEVENRYWLVVAARKNLELERTALDLAVDLKNRNQIQVEVGV
                     LPPVSVTQAESEVAAREVSVIRAENDLQAAEDNLKNTLAMDLSQRIAAVDEPTTEVYT
                     FSEQDSLAQAYEQRPEIQQAELDIANKKTLKHYYSNQRLPRFAVEGSLSLQGLGGDEN
                     PDRLSFEDEPEPIPPDFNSPSDAFRQLWNGDFATWQVLGIFSFPIFNRTASGNYIKAT
                     ADLDRSVIVLSRTKDDVALDVKSAVRQIENSLRAIDAARVSIKLAKEVVANEQERLNV
                     GIGTTRDVLEAQRDLIDAGTREITAVTSYNIALAELDYAKGTMLEKHGVRIEEFGPGE
                     MKPAPE"
     gene            3969..4625
                     /locus_tag="VFJ67_04380"
     CDS             3969..4625
                     /locus_tag="VFJ67_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7289655.1"
                     /translation="MSTEKALDKRELSAKEAAKDKVNTHDRILVAAIRLFSEKGFNGT
                     TTKEIAETAEVNEALIFRHFSTKRDLYGAIIERKISEEPGIELPLETHRDTKDDRLIF
                     TAIATHMFEKCGSDPSFMRLLHFSALEGHDLSDMFFDTYVEYINMLISDYIETRIKEG
                     EFKKLNPLYSSKAFIGMIVNQIIVQELFGEKKKNNTNHKELIQTFVEIFLEGIKSKKS
                     "
     gene            4803..5681
                     /locus_tag="VFJ67_04385"
     CDS             4803..5681
                     /locus_tag="VFJ67_04385"
                     /inference="COORDINATES: protein motif:HMM:NF025185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HET7289656.1"
                     /translation="MKNSFTDWIPLVLLPALAIFVARPALPAWGTLFAVAFSIFYGFK
                     WLTYRRAVAMGARPGLKSSVGYLFCWVGMDAAAFFDRRVSVPGPAGTEWLLALLKSAL
                     GLVLFFLAARIFYPVHALTAGYIGLTGFLMFSFFGTFHILSLYWRSRGVNAVPMMNSP
                     LLTASLSEFWSSRWNLAFRDIARIFVFRPVLRRYGIVYAVFAAFIFSGILHELLISLP
                     ADAWYGLPTLFFIIQACGVFIEKSESGVKHGINRGPRGRLFAAVFILVPLVLLFHPPS
                     IENCMLPFMKALGAIG"
     gene            5741..6361
                     /locus_tag="VFJ67_04390"
     CDS             5741..6361
                     /locus_tag="VFJ67_04390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HET7289657.1"
                     /translation="MLFYVKNKAVLSLAAVLVIALVSLLLKHILGLSWNLHIGGVTVA
                     LFFLCLGLILASDLILSILLVVIFHTAFIRLWMEMAGYFSRQRLYDVLAGGLLASGEE
                     MFFRGVLLQYMVQTLGWDSYYAVAISAAAFALFHVIWNKRLALFSVWAFWEGVMLGAA
                     YLYTDSLPAVMAVHAAHDIAGFTLFFLQRRRGFLVVIQHPGFRVTR"
     gene            6411..>6989
                     /locus_tag="VFJ67_04395"
     CDS             6411..>6989
                     /locus_tag="VFJ67_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7289658.1"
                     /translation="MNYESELKVAVEAVRKASGLCVRVQSSLVSEETVRKKDDSPVTV
                     ADFGAQAVICRELMKSFPDIPIVAEEDSSELGSEGGKALTARVLEFAAEVFPGMDEEG
                     LVSAIDAGDYGGGAGGTFWTLDPIDGTKGFLRGEQYAVALALIENGRVVLGVLGCPAL
                     PLDLKLPDGGKGCILTAVKGEGASMRPLEHNTP"
BASE COUNT         1512 a         2000 c         1958 g         1519 t
ORIGIN      
        1 ggcgcagtcg tccatgataa tcctcacgac ggtgacggac atagtagggt ttgccgtctt
       61 cctcggattg gctacaatca tgttcgggtt gaacaaatga gaggggaatt atgcccggga
      121 aaagggtaaa gctgctcata ggcaggaagg aaaaagtcga tttcccgacg ctcaggctct
      181 acggcataga cgccaaagtg gacaccgggg cctatacctc ggccatccac tgcgacagca
      241 taagggcagt gcggaaggga ggggagaggt tcgtcaggtt caggctgctc gacccctcgc
      301 acccggccta cgacgggagg gagatcagga agccgctcct cgggcggaag caggtcaaga
      361 attccttcgg gcagactgag ttcagataca tagtcaaaac ccgtatcgtg atattcggca
      421 agagccacga catagagctt tcgctatgcg acaggtcagg gatggagcac ccgatactgc
      481 tcgggaggaa attcatacag gacaagttcg ttgtggacgt atcgaggttc gacctgtccg
      541 cgaagaacag ggtcattaga atcggaagca acgggaaacc taaaggaagg aagccagggg
      601 cgcgcggtgg gaccgagcca gattcaagaa aataaaaaac acaatcagcg gtctattaca
      661 ctcatcaggc tataacatcc acggcctgct tcattagctc ctcggggaac ctcgcaaccc
      721 tgccgtccac gaactggacc ctgtaggatt tcccgtgcgg caggtcgaac tcctcgacga
      781 tcattccctt gagcccctgc tcgaacctgt cctttttccc ctctatgggc tggttgagta
      841 tgacctgcaa ccctttcctg aacatcccgc acctccggct gataaaaata atcgaatgag
      901 aatatataaa atcggcggag gatttgcaaa ttgggggagt tgtcgttgcg agccgaaggc
      961 gcggcaatct catgaataaa gcgagatcgc cacgaccaaa atacggtctc gcgatgacat
     1021 aaagatgccc cttcccgacg acgctttaca tcgattcccc aatcgttata taatgtgatg
     1081 acggaggtga cgccgatggc agagttcctc gataagctca aggtcaggct cgataagggg
     1141 ctcgagaccg taaatgtaaa atccaaggag ataatcggga ggcagaagat taggctccac
     1201 ctctcggagc tcgaggcgga gaggaagatg gtcttccagg acctgggcaa gctcgcctac
     1261 aagcagtatc agctctcggg cgacgcgccg ggattcgcgg ccggacaggc ccccctgacg
     1321 atcacctccc ccggcctcga caagtgggag gaatccgtcc tcgacgtgct cggcaggatc
     1381 tcggacgggc agctcaatgg gatacttttc gaggacaccc cgaagcagtt caaggtcacg
     1441 gccaagaccc tttacaaaga ggtagagaag ctaataccgg attcggagaa gaaggggcag
     1501 aacctccagt ggttaggccg cgtgctcggg aaattcaggc tcgcagcgaa aaaggtctcg
     1561 aagcggatcg acggcgagcg cgaaaccgtt tatgtgttcg acaagcagaa aacgcgctcc
     1621 gcgctcgaaa gcgtgaagtc gggcgcggaa aagagcgaaa agcccgccaa agagacagcg
     1681 tccccatccg atacggacgc cattctggtc tcgtgcaaaa agctcgcgga tattgactcc
     1741 aggattgaaa agctcaaaaa gcagatgaag aagatcggcg aaaaggcttg aacgcaaggc
     1801 ctttttaccg ggttataccc ctcagagcgc cttaccgacc accctctcta tctctccctt
     1861 gggaaccgcg cctatgatct gctcgacggc cttccctccc ttgaaaacga taagcgtggg
     1921 gatgctcctg attccgaatt tcatcgtggt ttgctgattt tcgtcaacgt tgagcttccc
     1981 gaccttgatc ctcccctgat aggaagacga gatctcctcg acggcggggg cgatagcccg
     2041 gcagggcccg caccaggggg cccagaaatc gaccagcacg ggcacgtcgc tcttcatgac
     2101 ctccgcatcg aagttcgtgt cgctcaattc gataattccg ttggcagcca taaatatctc
     2161 cttttcttta tactactctc tatacccgca attagtatat cagaaaactc ggtcgtctca
     2221 cagagccccg tctattcggg agcgggtttc atttcgcccg ggccgaactc ctcgatgcgg
     2281 accccgtgct tctcgagcat cgtacccttc gcgtagtcga gctcggcgag cgcgatgtta
     2341 tagctcgtga ccgctgtgat ctccctcgtg cccgcgtcta taaggtccct ctgcgcctcg
     2401 agcacgtcgc gcgtggtgcc tatcccgaca ttcagcctct cctgctcgtt ggcgacgacc
     2461 tccttggcga gcttaatcga caccctcgcg gcgtctatcg ccctcaaact gttctctatc
     2521 tgtctcaccg cgctcttcac gtcgagcgcg acgtcgtctt tagtccggct cagcacaatg
     2581 acgctcctgt cgaggtcggc tgtcgccttt atgtaattcc cgctcgctgt cctgttgaaa
     2641 atcggaaagc tgaatatgcc cagtacctgc cacgtggcga agtccccgtt ccagagctgc
     2701 ctgaaggcgt cgctcggact gttgaagtcg ggcgggatgg gctcgggctc atcttcgaag
     2761 ctgagcctgt cggggttctc gtcgccgccc agtccctgga gagagaggct gccctcgacg
     2821 gcgaacctcg ggagccgctg gttcgagtaa tagtgcttga gcgtcttctt gttcgcgatg
     2881 tcgagctccg cctgctgtat ctccgggcgc tgctcgtacg cctgggcgag tgagtcctgc
     2941 tcgctgaatg tgtagacttc tgtggtaggc tcgtccacgg cggcgatccg ctgggagagg
     3001 tccatcgcta gagtgttttt aaggttgtcc tcggcggcct gaaggtcgtt ctccgcccgg
     3061 atgacgctca cctccctagc cgcgacctct gactccgcct gcgtgacgga gacgggaggc
     3121 agcacgccca cttccacctg tatctggttc cggttcttga ggtcgaccgc tagatcaagc
     3181 gcggtgcgct cgagctcgag gttcttcctc gcggcgacga cgagccagta gcgattctcc
     3241 acttcgagga gcacctgcga tatcctcacc tccagctcct tcaccgttat ctcgttcgtc
     3301 cgcgtggccg tgatgatgaa cgtcctgttc acgtccacgc cgaaattcct gagcagctcc
     3361 tgcccgaggc tgaagcccac gttgttgaac cagctcgggc tcaggtcctc gagcgggttg
     3421 gtcgtcttgg tccacgtcgt attgaaatcg atcaggttgt agaacgtgcc gctcggcagg
     3481 agcccgttga tattggcttc cgcgttgaac tgcttctgag tgaccgtgcc gctcgggacg
     3541 aacgtgctta gcacgggctc ctccccgtcg ttgaagaacg aaacgatatt gaacagcgga
     3601 tcgaacacgc cctcctgcgt cttgatctcc ccttcagaga cagcgacgtt cttttcctct
     3661 atctgtatgt ccctgttatt ttcgagcgct atgcggacgg cgtcgttaag agagaggtcc
     3721 atgacgcccc ctgttccggt catcgagatc aaaatcagca acagcgtttt ggtcatcgta
     3781 atctctgaga aagtcgctcg ctcgggggcg cgcgcaataa attaaagttt caggttacaa
     3841 tatataattg aaaaaatcag gatttcaatc ggcctctgca gccggtcgga cagttttctt
     3901 gacactccat ggcatcgctg gtaatttgta agtaattact tacttacagc ctacagggat
     3961 aaaacgaaat gtcaacagaa aaagcgctcg acaagagaga gttatcggcc aaggaagccg
     4021 cgaaggacaa ggtcaacacg cacgacagga tactcgtggc ggcgataagg cttttctccg
     4081 agaagggatt caacgggacg acaacgaagg agatcgccga gaccgccgag gtgaacgagg
     4141 cccttatatt caggcacttt tccacgaaga gagacctcta cggggccatc atcgagagga
     4201 agattagcga ggagccgggg atagagctcc cgctcgagac gcacagggac acgaaggacg
     4261 accgtctcat tttcacggcg atcgcgacgc acatgttcga gaagtgcggg agcgacccgt
     4321 cgttcatgag gctcctgcat ttcagcgcgc tcgaaggcca cgacctctcc gacatgttct
     4381 tcgacaccta cgtcgagtac atcaacatgc ttatatccga ttacatcgag accaggatca
     4441 aggaagggga gttcaagaag ctcaacccgc tatactcctc gaaagcgttc ataggcatga
     4501 tcgtgaacca gataatcgtg caggagctgt tcggggaaaa gaaaaagaac aacacgaacc
     4561 acaaggaatt gatccagacg ttcgtcgaga tttttctcga aggcataaaa tccaaaaaat
     4621 cctgaagcgg gtcgaaatta tttgcgccga tccgcagacc gtatgcgccc gggccgcttc
     4681 cggggcgatg gacgagcgat aatccggaaa tagctttctt ccgcgcagcc gcacgcagcg
     4741 tatttcacgc ttcttataaa ttatcaatcc gaattccggc agcgtccgta ttaatcagac
     4801 aaatgaagaa ctccttcacc gactggatac cgctcgttct gctcccggcg ctcgcaatat
     4861 tcgtagctcg tcccgcgctc ccggcgtggg gcactctctt cgcggtcgca ttctcgatat
     4921 tctacggttt caagtggctc acatacagaa gggccgtagc catgggagcg agaccgggac
     4981 ttaagagctc ggtcgggtat ctcttctgct gggtgggaat ggacgccgca gcctttttcg
     5041 acaggagagt cagcgtcccc gggccggcag gaacggaatg gctcctcgct ctcctcaagt
     5101 ccgcgctcgg gctcgttctc ttctttctcg ccgcgcgcat cttttatcca gtccatgctc
     5161 tgaccgcggg ctacataggg ctcacggggt tcctgatgtt ctctttcttc ggcacgttcc
     5221 acatactgtc actttactgg aggagccgcg gcgtgaacgc tgtcccgatg atgaactcgc
     5281 cgctgctcac ggcctcgctc tccgaattct ggagcagcag gtggaacctc gccttccgcg
     5341 acatagcccg catattcgtg ttcaggccgg tgctgagacg gtacggtatc gtgtacgcgg
     5401 tctttgcggc gtttatattc tcaggcatcc tccacgaact tctcatatcg ctcccggccg
     5461 acgcgtggta cggcctcccg acactcttct tcatcattca ggcgtgcggg gtcttcatag
     5521 agaaatcaga atcaggcgtg aagcacggaa tcaaccgcgg accgagggga cggctctttg
     5581 ccgctgtatt catactcgtc cctctcgtgc tgctctttca cccgccgtcg atagagaact
     5641 gcatgctccc gttcatgaaa gccctcggcg ccataggctg acaggatgga cttgcagctc
     5701 ccacgccgtt aaactgaacg ggttaccgga tagaagggcc atgctgttct acgtaaaaaa
     5761 taaagccgtc ctctcgctcg cggcggtgct cgtaatcgcg ctcgtctctc tcctgctgaa
     5821 acacatcctc ggccttagct ggaaccttca cataggcgga gtaacggtcg cccttttttt
     5881 cctgtgcctc ggccttatac tggcttcaga cctgatcctc agcatccttc tcgttgtcat
     5941 tttccacacc gcgttcatcc gcctgtggat ggagatggcc gggtatttca gccggcagcg
     6001 cctctatgat gttctggcgg gaggcctcct cgcgtcgggc gaggagatgt tcttcagggg
     6061 cgtgctgctc caatatatgg ttcagaccct cggctgggat tcatattatg ctgtcgccat
     6121 atccgcagcc gcgttcgccc tcttccacgt aatatggaac aagcgcctcg cgctcttctc
     6181 cgtctgggcg ttctgggaag gcgttatgct cggggcggcg tatctctaca cagattctct
     6241 cccggccgtt atggccgtcc acgccgcgca cgacatagcg gggttcacac tcttctttct
     6301 ccagaggagg agggggtttc tagtcgtgat acagcatccg gggtttcggg taacgagata
     6361 aatggtaaaa tgtttctcct cataatacgg aataaaatca ggagcagcgg atgaattacg
     6421 aatccgagtt gaaagttgcg gtcgaagccg tgagaaaggc ctccgggctg tgcgtgaggg
     6481 tgcagtcctc gctcgtatcg gaagagacgg taaggaagaa ggacgactcg cccgtcacgg
     6541 tcgcagactt cggcgcgcag gccgtaatat gccgtgagct catgaaatcc ttccctgata
     6601 tcccgatcgt cgccgaagag gactcatccg aattgggatc cgagggaggg aaagccctca
     6661 ccgcgcgcgt actcgaattc gccgccgaag tatttcccgg aatggacgaa gaaggtctcg
     6721 tctcggcgat agatgcgggg gactacggcg ggggagcggg cggaacgttc tggacgctcg
     6781 accccataga cgggacgaag ggattcttga ggggggagca gtacgcggtt gcgctcgctc
     6841 tcatcgagaa cggccgcgtg gtgctgggcg tgctcggctg ccctgccctg ccgctcgacc
     6901 tcaagctccc ggacggaggc aagggctgca tactcaccgc tgtgaagggc gaaggcgcgt
     6961 cgatgcgtcc tctcgagcac aataccccg
//
LOCUS       DASSJH010000205         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_51043, whole genome shotgun sequence.
ACCESSION   DASSJH010000205 DASSJH010000000
VERSION     DASSJH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51043"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VFJ67_04400"
     CDS             complement(<1..1551)
                     /locus_tag="VFJ67_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7289659.1"
                     /translation="MSRRMVIGKLSDFENDGMKTVGLGDSKDKILICRFGGEFSALSP
                     YCTHYGAPLEEGALSEGRIVCPLHHSCFSAKNGDFLEPPAFDSLVAYELESEGENLVV
                     TVPDKIEKRRSPGLAKPDYKSDKRRFGIIGGGAAGYSAAQTLRENGFKGRIYIITREN
                     ELPYDRPNLSKEYLQGKAEADWMPLRGQKFYDEHGIDIVGGREVLKLDVQNKKLTLDE
                     GQDLVFDKILIASGGKPLNLNIPGADLGNIFTLRSFANADEIIAAAEKASKAAVIGSS
                     FIGMETAYSLSVLGINVTVIGKDSVPFENILGKEIGELFMKEHGNRGIAFEMSANVSR
                     FAGSGKVEKIVFNDGKEIPADLVLVGIGVVPATGFIEGIGLNPDGSLRTDIYLAVAED
                     VYAAGDIASFPNAVTGERRRIEHWRTAEQQGKIAALNMLGKNVPYSSVPFFWTAQAGL
                     ELRYVGHARAWDYTITWGSVADKDFITFYISGDKVAAAAGNNRDAEMAAAEELMRTGR
                     MPAPGVLEK"
     gene            1786..>3337
                     /locus_tag="VFJ67_04405"
     CDS             1786..>3337
                     /locus_tag="VFJ67_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7289660.1"
                     /translation="MALIGKPSVIAPPAETGTETASARRTSGRWVLAATILGSSIASI
                     DGTAVNVALPALQEKLGATITNAQWVIEAYTLFLAALILVGGALGDRYGRRRVYALGV
                     ALFALSSVLCGLSHSIYQLIAFRALQGVGGAMLIPGSLAIITVFFSPGERGRAIGTWS
                     AFSAITTALGPVLGGWLIENVSWRWIFFINVPIAAAVLTILFLRVPESRGGVSSGRVD
                     WAGGVLATLGLGGILFGLIESSNLGFGSPLVMATLALGALALVSFVIVEGREESPMMP
                     PSLFRSVTFTGTNVATLFMYGALGGVFFFLPFALIQVYGYTATEAGASLLPIILIIFV
                     LSRWAGGLTDKYGAKIPFAAGTVLGAAGYLSLALFNGEGSYFSTIFPGITLIGLGLAL
                     SIAPLTTAVMNSVHVEYSGTASGINNAVARMAGLLTIAVLGIIILHAFNGYLDAGMDA
                     ASVPRAVRDLLAGERIKMAGAEIPPALGLEMAATLRGIVRGSFIGGFDIVLYIASGFS
                     LLAAAVGLA"
BASE COUNT          592 a         1016 c          965 g          764 t
ORIGIN      
        1 tttttcgagg accccgggcg cgggcatcct gccggtcctc ataagctctt ccgcggcggc
       61 catttccgcg tccctgttgt tccccgccgc ggccgccacc ttatcccccg atatgtaaaa
      121 cgtaataaaa tccttatccg cgacgctccc ccacgtaatc gtgtagtccc aggcacgggc
      181 gtgtccgacg tatcttaatt ccagtcccgc ctgcgcggtc cagaagaacg gcacgctcga
      241 ataaggaacg tttttgccga gcatgttgag cgccgcgatt ttcccctgct gctccgccgt
      301 cctccaatgc tcgattcttc ttcgttctcc cgtaacggcg ttcggaaacg aagcgatgtc
      361 gccggccgca taaacgtcct ccgccacggc gaggtaaata tccgtcctga ggctaccgtc
      421 cggattgagc ccgatacctt ctataaatcc ggtcgccggt actaccccta tgccgacgag
      481 gacgaggtcg gcgggaatct ctttcccgtc gttgaatact attttttcaa cttttccgct
      541 ccccgcgaat ctcgacacgt tcgcgctcat ctcgaacgcg attccgcggt tcccgtgctc
      601 tttcatgaat aactccccga tctcttttcc caatatattt tcgaatggaa cggagtcctt
      661 gcctattacg gtaacgttta taccgagcac ggacaggctg taagcggttt ccatgccgat
      721 gaagctcgat cctataaccg cggctttgga cgccttttcc gcagcggcta ttatctcatc
      781 ggcgttcgcg aagcttctga gcgtgaagat attgccgaga tccgctccgg ggatgttgag
      841 gttgaggggt ttcccgcccg aggcgatgag aatcttgtcg aacacgaggt cttgtccctc
      901 gtcgagcgtc agctttttgt tctgtacgtc cagcttcaaa acctctctcc cgccgactat
      961 atcgataccg tgctcgtcgt aaaatttctg acccctgaga ggcatccaat ccgcctcggc
     1021 cttgccctgc aggtactcct tacttaaatt cggccggtca tacgggagtt cgttctctct
     1081 cgttattata taaatcctgc ccttaaagcc gttctcccgg agcgtctggg cagccgaata
     1141 gccggccgct cccccgccga ttataccgaa cctcctcttg tcggatttat agtccggttt
     1201 ggcaaggccg ggcgaccgcc ttttttcaat tttatcgggg accgtgacga caaggttctc
     1261 gccttcggac tcgagctcgt atgcgaccag agaatcgaat gccggcggct cgagaaaatc
     1321 cccgttcttc gcgctgaagc atgaatggtg gagcgggcag acgatcctgc cttcgctcag
     1381 cgccccttct tcgagcggag ctccgtagtg ggtgcagtat gggctcaggg cggaaaattc
     1441 cccgccgaac ctgcaaatca gtatcttgtc cttcgaatcc ccgagcccga cggttttcat
     1501 gccgtcgttc tcaaaatcgc tcaatttccc tattaccatt cgcctgctca taattgcctc
     1561 cgtacaagcg caatatgtcc tgtcatcagt gtacttcaaa cgttatacca caagaccgcg
     1621 gctattaaaa ccggacaaat aactgttaat cacattatag gctgtgcgaa cgggttgggt
     1681 gcggtgccgg ttaacagaag agctcgggaa gaatcgtata tgagcgcaca ttgtgcgcgt
     1741 gtgccggttt acatttgtgg agtatagtgt ttactttacg gctttatggc tctgatagga
     1801 aaaccctccg tcattgcgcc cccggccgag accggaaccg agaccgcttc cgctaggagg
     1861 acctcgggaa ggtgggtgct tgcggcgacc attctcggat cgagtatcgc gtccatagac
     1921 ggcaccgcgg tgaacgtcgc gctgccggcg cttcaggaaa aactgggcgc gacgataacg
     1981 aacgcccagt gggttataga ggcgtacacg ctgtttctag cggcgctcat actcgtcggc
     2041 ggagcgctcg gggacaggta cgggaggagg cgcgtctatg cgctcggcgt cgcgctcttc
     2101 gccctgtcgt cggtcctctg cggactctcg cattccatat atcagcttat cgcgttccgc
     2161 gcgctccagg gcgtgggcgg ggcgatgctc ataccgggga gcctcgcgat aatcaccgtc
     2221 ttcttcagcc ccggggagag ggggagggcc ataggcacgt ggtcggcgtt ctcggcgata
     2281 acgaccgcgc tcggcccggt gctgggcgga tggctcatcg agaacgtctc ctggcggtgg
     2341 atattcttca tcaacgtgcc gatagcggca gccgtgctca cgatactgtt cctccgcgtg
     2401 cccgagagca ggggcggcgt gtcgagcggc agggtggact gggcgggcgg ggtgctcgcg
     2461 acgctcgggc tgggcggcat actcttcggc ctcatcgaga gctcgaacct cgggttcggg
     2521 agcccgctcg tgatggcgac actcgcttta ggcgcgctgg cgctcgtgtc gtttgtcatc
     2581 gtcgagggga gggaggaatc gcccatgatg cccccttcgc tcttccgctc ggtcacgttc
     2641 acgggcacga acgtcgcgac gctcttcatg tacggcgcgc tggggggagt gttcttcttc
     2701 ctgcccttcg cgctcatcca ggtgtacggc tacacggcta cggaggccgg ggcgtcgctc
     2761 cttcccataa tactcatcat attcgtgctc tcccgctggg cgggcgggct caccgataaa
     2821 tacggggcga agataccgtt cgccgcggga accgtgctcg gggcggcggg ctacctttca
     2881 ctcgcgctat ttaacggaga ggggagttat ttcagcacca tattccccgg cataacgctc
     2941 atcggcctcg ggctcgcgtt gagcatcgcg cccctgacga cggcggtaat gaactcggtc
     3001 cacgtcgagt attcggggac cgcgtcggga ataaacaacg ccgtcgcgag gatggcgggc
     3061 ctcctcacga tagcggtgct cggcataata atactccacg cgttcaacgg ctacctcgac
     3121 gcgggcatgg acgctgcttc cgtgccccgg gccgtgaggg acctgctcgc gggggaaagg
     3181 ataaagatgg ccggagccga gataccgccc gcgctcggcc tggagatggc cgctacgctc
     3241 cgcgggatcg tacggggctc gttcataggc gggttcgaca tcgtgctgta tatcgcgtcg
     3301 ggcttctcgc tcctcgcggc tgcggtcgga ctcgcta
//
LOCUS       DASSJH010000206         7544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1349402, whole genome shotgun sequence.
ACCESSION   DASSJH010000206 DASSJH010000000
VERSION     DASSJH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349402"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="VFJ67_04410"
     CDS             <1..105
                     /locus_tag="VFJ67_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET7289661.1"
                     /translation="LRTNIGIVSTLDDAVADFNRTERRKGKTIVRVRP"
     gene            321..1574
                     /gene="icd"
                     /locus_tag="VFJ67_04415"
     CDS             321..1574
                     /gene="icd"
                     /locus_tag="VFJ67_04415"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020278096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HET7289662.1"
                     /translation="MSSSKPSVGQKIKIDKKNESLIVPDNPIIPFIEGDGTGPDIWRA
                     SQIVFDAAVEKAYKGKRKVMWREVLTGEKAFNLKGEWLPEETLEDLKEYTVSIKGPLT
                     TPIGGGIRSLNVALRQLLDLYACVRPVKYIKGTPSPIKRPEDMDMIIFRENTEDVYSG
                     IEWQSGSKEAIDIRNYLVDKYGVNIREGSGIGIKPISPFGTKRLVRKALKYAVEKKKE
                     SVTIVHKGNIMKFTEGAFRDWGYELAREEFSKEVITEDDLWDKYGGKRPEGVVVINDR
                     IADNMLQQILTRTREYHVITTANLNGDYLSDACAAQVGGLGMAPGGNIGDYLALFEAT
                     HGTAPKYAGQDKVNPGSLILSGVMMFEYLGWNEAADLIVKAMELTILDKTVTYDLERQ
                     LEGAKLLKCSEFGETIAANMDRVLV"
     gene            1650..2591
                     /gene="mdh"
                     /locus_tag="VFJ67_04420"
     CDS             1650..2591
                     /gene="mdh"
                     /locus_tag="VFJ67_04420"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7289663.1"
                     /translation="MSRPKISVIGAGNVGATTAMMLSQLEIGDVVLVDIVEGMPQGKA
                     LDMMEMNPVYGLDVNITGTNGYEETANSDVVVITSGIPRKPGMSRDDLIATNTEIVKQ
                     VTENVAKRSPNAILILVTNPLDAMVYVAHKVSKFPKERVMGMAGVLDSARFRSFIGME
                     LGVSVENIEAFVLGGHGDDMVPLARCTTVAGVPITELIPEDRIESIIKRTRGGGGEIV
                     ALLKTGSAFYAPAVSAVEMVEAILKDKKKILPCCVLAQGEYGVDNVFVGLPVILGRKG
                     IEKIFEIKLTKKESEELKVSASHVKELCDHIDKTTAK"
     gene            2737..3468
                     /locus_tag="VFJ67_04425"
     CDS             2737..3468
                     /locus_tag="VFJ67_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HET7289664.1"
                     /translation="MANGTAQNVMTLRRDAWWVAPLVTAFILGAFGIYSTWRAFSNAD
                     FIYGPYISPFYSPYILASWWPFSPALLILWAPLGFRATCYYYRKAYYRSFFLAPPACA
                     VKPLAKEGSYCGETKFPFIIQNIHRYFFYAATVILFFLWVDAFKALKFEDGFGVGVGT
                     LVLFANATLLSFYSLGCHSFRHLIGGKLDVFSKCPTRFMLWSKVSVFNEHHMLWAWVS
                     LFGVALADLYVFLLARGVITDVRLI"
     gene            3469..5196
                     /locus_tag="VFJ67_04430"
     CDS             3469..5196
                     /locus_tag="VFJ67_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HET7289665.1"
                     /translation="MEKYETHTHDVIVIGAGGAGLRAAIEASAQGASVGLVCKSLLGK
                     AHTVMAEGGVAAALANLDAQDNWKVHFHDTMHGGKMLNNWRMAQLHAQEAPDRVRELE
                     HWGALFDRTKDGKIMQRAFGGHTWKRLAHVGDRTGLEMIRTLQDKGIHTGMDVYMECT
                     ITHLLKDGERISGAFGYWRESGRFVLFKTKAVILATGGIGKSYKVTSNSWEYTGDGHA
                     LAYDAGADLIDMEFVQFHPTGMVWPPSVKGLLVTEAVRGEGGLLTNRNGERFMEKYDP
                     ERMELSTRDVVARSIYIEVQEGRGSPHGGAFLDISHRGAEYVKKKLPSMYHQFMEFAE
                     IDITKEPMEVFPTTHYMMGGVRVDADTTGSTVPGLFAAGEAAGGMHGANRLGGNSLSD
                     LLVFGRRAGLGAAQYANELRDNTLKIDDVHLENAAQEMLKPFENAEGENPYTVHQALQ
                     EAMGSYVGVFRTKEKLQDGLEEIQVLKDRASQLRIEGSRMYNPGWHLCKDLKSMLIVS
                     EAIAKCALQREESRGAHSRVDFPKADYDKWGKVNSVISKDNGNMKISTSPLPQMPEEL
                     RKIVEGGHK"
     gene            5197..5937
                     /locus_tag="VFJ67_04435"
     CDS             5197..5937
                     /locus_tag="VFJ67_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET7289666.1"
                     /translation="MAVVKMRIYRGDSNGGSEKEYEVPLDEGMVVLDAIHFIQANYDG
                     DLAVRWNCKAAKCGSCSAEINGMPKLTCKTRLDSLPLDKPVTVYPIKSFPIMKDLVTD
                     VSWNYKVRKTIPPFTPAKDTEWIMYQEDVERSQEFRKCIECFLCQNVCHILRNHDKKN
                     EFIGPRMLIKIAELEMHPLDVEDRKAFLRGQAGVGYCNITKCCTEVCPEHIHITDNAI
                     IPLKERVADTYFDPLQWLFGRSKGNHKQ"
     gene            5965..7128
                     /gene="sucC"
                     /locus_tag="VFJ67_04440"
     CDS             5965..7128
                     /gene="sucC"
                     /locus_tag="VFJ67_04440"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET7289667.1"
                     /translation="MNIHEYQSKELLSQYGVATPTGKVAFKEDEAHQIAKDLNADRFV
                     VKAQIHAGGRGKGGGVKLASTLDEVKQIASEILGMILVTHQTGPEGKLVQKVLVEEAS
                     DIEKELYLGMVIDRSREQIVIMASREGGMEIEEVARKHPSKITREYVDPTVGLLPYQC
                     RKLAYFLGLEGKTVNKAVKFISGLYQLFTEKDCSLAEINPLILTKGGDVLALDAKMNF
                     DDNALFRHPDIEKLHDPSEEDPTELEAKKWGISYVKLDGNIGCLVNGAGLAMSTMDII
                     KHHGGEPANFLDVGGGANTEQVTQAFKMILSDPNVKAIFVNIFGGIMKCDTIAEGIIA
                     ASKEVGISVPLVVRLEGTNVELGRKMLSESGLNIVTGGDMREAAKNVVEAAGA"
     gene            7326..>7544
                     /locus_tag="VFJ67_04445"
     CDS             7326..>7544
                     /locus_tag="VFJ67_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HET7289668.1"
                     /translation="MKAVAVREPIGVFLQEVNKYPVLSKEEEYSLAVRYYEENDLEAA
                     NKLVVSNMRFVIKIASEYISYGFPLADLV"
BASE COUNT         1961 a         1948 c         2227 g         1408 t
ORIGIN      
        1 ctgcgcacga acatcggcat cgtctcgact ctcgacgatg ctgtcgccga ctttaaccgg
       61 accgagcgac gtaaggggaa gacaatcgtc cgtgttcgtc cgtagggaca tgcgcccgtg
      121 gtatactgca gaccgtactc agacagatga tgaagcggta ggcccgtccg aacacccgcg
      181 ctgagtatgt ggaatttgtc cttctagcga gggtacgagt ccgcccggac gctcgttgag
      241 agcggaaagt gctcaataat ttgaattccg ggcttttatt atttatattt cattccaaat
      301 tttttaacag gagttttacg atgtcatcct caaaacccag tgtgggtcag aaaataaaga
      361 tagacaaaaa gaacgaatcc ctcatagtgc ctgacaaccc cattatccca ttcatagaag
      421 gcgacggcac cgggcccgac atttggaggg cttcccagat agttttcgac gccgccgtcg
      481 agaaggcgta taaaggaaag agaaaggtaa tgtggcgcga ggtgctcacg ggggagaagg
      541 cgtttaatct caagggcgaa tggctgcccg aggagacgct cgaagacctt aaggaatata
      601 ctgtgtccat caagggcccg ctcacgacgc cgataggcgg tggcataagg agcctgaacg
      661 tcgcgctgag acagcttctc gacctctacg cgtgcgtccg ccccgtaaag tacattaaag
      721 gaaccccgtc cccaataaag agacccgagg atatggacat gatcatattc agggagaaca
      781 cggaggacgt ttattccggc atagagtggc agagcggctc gaaggaagcg atagatataa
      841 ggaactacct ggtcgataaa tacggggtga acataaggga aggctccggg ataggcataa
      901 agccgataag ccccttcggt acgaagaggc tcgtcaggaa ggcgctcaaa tacgcggtcg
      961 aaaaaaagaa ggaaagcgtc acgatcgtgc acaaaggcaa tatcatgaag ttcactgaag
     1021 gcgctttcag ggactggggc tacgagctcg cgagagaaga attctcgaaa gaggttatca
     1081 cggaggacga cctctgggat aaatatggcg gcaagagacc ggaaggggtc gttgtaataa
     1141 acgacaggat cgcagacaat atgctccagc agatactcac gaggacgagg gaataccacg
     1201 tcataactac ggcgaacctg aacggcgact acttatccga tgcctgcgcg gcgcaggtgg
     1261 gaggcctcgg catggctccc ggcggcaaca tcggcgacta cctggcgctc ttcgaagcga
     1321 cccacgggac cgctcccaag tacgcgggtc aggataaggt gaaccccggc tcgctgatat
     1381 tatccggcgt gatgatgttc gaatatctgg ggtggaacga agccgccgac ctcatagtaa
     1441 aagctatgga gctcacgata ctcgacaaga cggtcacgta cgacctcgaa cgccagctcg
     1501 agggcgcgaa gctgctcaag tgctctgagt tcggggagac gatagccgca aacatggaca
     1561 gggttctggt ttagctgttg atcggttata caagttacgg gatataagca cctgaaaaac
     1621 tataaagaaa taataaagga gcatttgtta tgagcagacc gaagatttca gtaattggcg
     1681 cggggaacgt cggcgccacg acggcaatga tgctgtcaca gctcgagatc ggagacgtag
     1741 tactcgtgga catcgtcgag gggatgccgc agggcaaagc gctcgacatg atggagatga
     1801 accccgttta cgggctcgac gtcaacatca cgggcacgaa cggttacgag gagacggcga
     1861 attctgacgt cgtagtcatc acctcgggca ttccgaggaa gcccggcatg agcagggacg
     1921 acctcatcgc aacgaacacg gaaatagtaa aacaggtgac tgagaacgta gccaagaggt
     1981 cgccgaacgc gatattgatc ctagtcacca atcctctgga cgcgatggtt tatgtcgccc
     2041 acaaggtgag caaattcccc aaggagcgcg tcatgggaat ggcgggagtg ctcgactccg
     2101 cgaggttcag gtcgttcatc gggatggagc tcggcgtctc ggtcgagaat atagaggcgt
     2161 tcgtgctcgg cgggcacggc gacgacatgg tcccgctcgc gagatgcacg accgtggcag
     2221 gcgtgcctat cacggagctc atccctgaag acaggataga atcaataatc aaaaggacca
     2281 gagggggcgg tggggaaatc gttgcgcttc tcaagacggg gagcgctttc tacgcgccgg
     2341 ccgtgtccgc ggtcgaaatg gtcgaggcta ttctcaagga caagaagaag atactgcctt
     2401 gctgcgtgct cgcgcagggt gagtatggag tagacaacgt tttcgtcggt ctgcccgtaa
     2461 tcctagggag gaagggcatc gagaagatat tcgagataaa gctcacgaag aaggagtctg
     2521 aagagctcaa ggtctcggcg agccatgtga aagagctttg cgatcatata gataaaacga
     2581 cggcaaaata agagacaaac ggacacgccc ggagggtctt tcggcttgac tagctccggg
     2641 cgttcatata atattacagg ccgattttcc cgtacgaata ttacgaatct ttgacactaa
     2701 aacacaattt ctgagacgag ttagggaggc acctaaatgg caaacggaac cgcgcagaac
     2761 gtaatgacac tgaggcggga cgcatggtgg gtggcgccgc tggtcacagc cttcatactg
     2821 ggcgcattcg gcatatactc cacgtggcgc gcgttctcga acgccgattt tatatacgga
     2881 ccttacatat ctccgtttta ttccccttac atactggcct catggtggcc tttctctccc
     2941 gcgctcctca ttctatgggc gccgctcgga ttcagggcca cttgctacta ttacaggaag
     3001 gcctattaca ggtcgttctt cctcgcaccg cccgcctgcg ccgtgaagcc cctcgcgaag
     3061 gaaggctcat actgcgggga gacgaagttc ccgttcataa tccagaacat acaccgctat
     3121 ttcttctacg ccgcgacggt aatactgttc ttcctctggg tggacgcctt caaggccctc
     3181 aagttcgagg acgggttcgg agtaggcgtg ggcactctcg tgcttttcgc gaacgccacg
     3241 ctcctcagct tttattccct gggatgccat tcattccgcc acctgatagg cgggaaactc
     3301 gatgtattct cgaaatgccc cacacggttc atgctatgga gtaaggtaag cgtcttcaat
     3361 gagcaccaca tgctatgggc atgggtgagc ctcttcggag tcgctctcgc cgacctctac
     3421 gtgttccttc tcgcaagggg cgttatcaca gacgtgaggt taatctaaat ggagaagtac
     3481 gaaacacata cgcacgacgt cattgtaatc ggcgcgggag gcgccggatt gagagcggct
     3541 atcgaagcgt cggcccaggg cgcgtcggta ggactcgtct gcaagtcgct ccttggcaag
     3601 gcgcacacgg tcatggctga gggtggcgtt gccgccgcgc tcgcgaacct cgacgcgcag
     3661 gacaactgga aggtccactt ccacgatacc atgcacggcg ggaagatgct gaacaactgg
     3721 cgcatggcgc agctccacgc gcaggaagcg cccgacaggg taagggagct cgagcactgg
     3781 ggggcgctct ttgacaggac gaaggacggt aagataatgc agagggcctt cggcggccac
     3841 acgtggaaga ggctcgccca cgtcggcgac aggaccggcc tcgagatgat acgcacgctc
     3901 caggacaagg gcatacacac cggaatggac gtgtacatgg aatgcactat tactcatctg
     3961 ttaaaggacg gcgagaggat atcaggcgcg ttcggctact ggagggagag cggcaggttc
     4021 gtgctcttca agacgaaggc cgtgatactc gccacgggcg gaatagggaa gtcgtacaag
     4081 gtcacatcca attcctggga atacaccggc gacggacacg cgctcgccta cgacgccgga
     4141 gcggatctga tcgacatgga attcgtacag ttccatccga ccgggatggt atggccgccg
     4201 agcgttaagg gattgctcgt caccgaggcc gtaaggggcg agggaggcct gctcacaaac
     4261 aggaacggcg aaaggttcat ggaaaaatac gaccccgaga ggatggaact ctctacgagg
     4321 gacgtcgtcg cgaggtccat ctatatcgag gtccaggaag gccgcggctc gccccacggc
     4381 ggcgcgttcc tcgacatatc ccacaggggg gccgagtacg tgaagaagaa gctccccagc
     4441 atgtaccacc agttcatgga gttcgccgag atagacataa cgaaagaacc tatggaagta
     4501 tttccgacta cccattacat gatgggcgga gtccgtgtgg atgccgacac gaccgggagc
     4561 acggtgcccg gactcttcgc cgcgggagaa gccgcgggcg gcatgcacgg cgccaacagg
     4621 ctcggaggga attcgctctc ggacctcctg gtattcggaa ggagggcggg tctcggcgcg
     4681 gcgcagtacg cgaacgagct ccgcgacaac acgctcaaga tagacgacgt acacctcgag
     4741 aatgccgctc aggagatgct caagccgttc gagaacgcgg agggcgagaa cccctacacc
     4801 gtgcatcagg cgctccagga ggcaatgggg tcgtatgtag gcgttttccg gacaaaggaa
     4861 aagctccagg acgggctcga ggagatacag gtgttaaaag acagggcctc tcagctcagg
     4921 atcgagggct cgcgcatgta caaccccgga tggcacctct gtaaagacct taaatcgatg
     4981 ctcatcgtct ctgaagccat agcgaaatgc gctctccaga gggaggagag caggggcgcc
     5041 cacagcaggg tggacttccc caaggcggat tacgataaat ggggcaaggt caattcggtg
     5101 atctcgaaag acaacgggaa catgaagata agcacgtctc cgctccccca gatgcccgag
     5161 gagctgagaa agatcgtaga gggaggtcac aaataaatgg cggtcgttaa aatgaggatc
     5221 tacaggggcg actctaacgg tggctctgaa aaggaatacg aagtacctct cgatgaaggg
     5281 atggtcgtcc tcgacgccat acacttcata caggccaact acgatggcga cctggccgtg
     5341 aggtggaact gcaaggcggc caaatgcggc tcgtgcagcg ccgagataaa cggcatgccg
     5401 aagctcacct gcaagaccag gctcgactcg ctgccgctcg ataagcccgt gaccgtttac
     5461 cctattaaga gctttcccat aatgaaggac ctcgtaacgg acgtctcctg gaactacaag
     5521 gtcaggaaaa cgataccgcc tttcactccg gcgaaggata cggaatggat tatgtaccag
     5581 gaggacgtag agaggagcca ggaattcagg aagtgcatag agtgcttcct ctgccagaac
     5641 gtctgccata tattgaggaa ccatgacaag aagaacgagt tcatagggcc gcgcatgctg
     5701 ataaagatcg ccgagctcga gatgcacccg ctcgatgtcg aagacaggaa ggcgttcctg
     5761 aggggccagg ccggggtagg gtactgcaac atcaccaagt gctgcacgga agtatgcccg
     5821 gaacacatac atataaccga taacgcgata atcccgctca aggagagggt ggccgatacg
     5881 tacttcgacc cgctccagtg gcttttcggc agatccaaag gtaaccacaa acaatagtca
     5941 aagtttttcc caggaggaat taaagtgaat atccatgagt atcagtcgaa ggagctgctc
     6001 tcccagtacg gcgtcgcgac gcccacggga aaggtcgcgt tcaaggagga cgaagctcac
     6061 cagatcgcga aagacctgaa cgcggacagg ttcgtcgtaa aggcgcagat tcacgcggga
     6121 gggagaggca aaggcggggg agtcaagctc gcctcgacac tcgatgaggt caaacagata
     6181 gcctccgaga tactcggcat gatactcgtt acgcaccaga cgggccccga ggggaagtta
     6241 gtacagaagg tcctcgtcga agaggcatcg gatatagaaa aagaactcta cctcggcatg
     6301 gtcatcgaca ggtcgaggga gcagatagta ataatggcga gccgcgaagg cggcatggag
     6361 atagaagagg tggctcggaa gcacccttcg aaaataaccc gtgaatacgt cgacccgact
     6421 gtgggcctcc tgccttatca gtgcagaaag ttagcctact tcctcggact cgagggcaag
     6481 actgtaaata aggccgttaa attcatctcg ggcctgtatc agctcttcac ggagaaggac
     6541 tgctcgctcg ccgagataaa cccccttatc ctcaccaagg gcggggacgt gctcgcgctc
     6601 gacgccaaga tgaacttcga cgacaacgcc ctcttcaggc acccggacat agagaagctc
     6661 cacgacccgt cggaagagga ccctacagag ctagaggcca agaagtgggg catcagctac
     6721 gtgaagctcg acggcaacat cggctgcctc gtgaacggcg cgggcctcgc catgtccacc
     6781 atggacataa taaagcacca cggcggggag cccgctaact tcctcgacgt cggcggagga
     6841 gccaacacgg agcaggtcac tcaggccttc aagatgattt tgagcgaccc aaacgtaaaa
     6901 gccatattcg ttaacatatt cggcggcatc atgaaatgcg acaccatagc cgagggcatc
     6961 atagcggcct cgaaagaggt gggcataagc gtgcctctgg tcgtcaggct cgaggggaca
     7021 aacgtcgagc tgggacggaa aatgctgtcg gaatcaggac ttaacatagt cacgggcgga
     7081 gacatgaggg aagccgccaa gaatgtcgta gaggcagcag gagcgtgatg aaccccgtgt
     7141 acccgcgttc ctctcgacag gccgggacgg ccccgtccac accccttgaa atttaaggaa
     7201 agtattaata tattagcact aaccatttgc catcactaga ataatatatt tcgacattcg
     7261 tctttttgcc cccttgacat ttccccgatg atgattagtt ttcaaaaagg aggtttaaaa
     7321 aacggatgaa agcagtcgca gtcagggagc cgataggtgt ttttttacag gaagtaaaca
     7381 aatacccggt tctgagcaaa gaagaagaat acagcctggc ggtgaggtat tacgaggaaa
     7441 acgacctcga agcggccaac aagctggtcg tatcgaacat gaggttcgtt ataaagatag
     7501 cgtccgagta tatctcctac gggttccctc tcgccgacct tgtt
//
LOCUS       DASSJH010000207         4763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_588543, whole genome shotgun sequence.
ACCESSION   DASSJH010000207 DASSJH010000000
VERSION     DASSJH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588543"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VFJ67_04450"
     CDS             complement(<1..988)
                     /locus_tag="VFJ67_04450"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7289669.1"
                     /translation="MDKIDGVQINDLAREFGSPLFVASASAIIRNCRAFRAAFSAAYP
                     NVEVAYSYKVNSVPALLGIIHNEGLWAEAASGFEYGLARRMGVPGSSIVLNGPYKKKE
                     ELKEALREGAIINADHTDELDVLEEIAREEGGHVEIGIRVNMETGIDQLPDRFGFSIE
                     SGDAMRAVERACSGGLLRIAGLHVHLTSYIIEPHDEGGMPAEYIKLIWPKDARAYRKA
                     ASETARFATEAREKFGVRMKYIDMGGGFPNVGSIAPYVSAVTEPILAACGGEEPPLLI
                     LEPGRAIVSDAVHLITTVATMKELRGGVRAAVADAGINILPTSFWRFQDIEYA"
     gene            complement(1018..1962)
                     /locus_tag="VFJ67_04455"
     CDS             complement(1018..1962)
                     /locus_tag="VFJ67_04455"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET7289670.1"
                     /translation="MKVSEDVLSLIGNTPLVRVNKLTGGLGSEIYAKLEFTNPSGSFK
                     DRVAKYIIDDAEARGLIKPGDLIVEATSGNMGVAFALVGAVRDYRCIFTLPETISKEK
                     ILAIKLFGAEVVLTPRGLPPSDERSCYKVAQRIAREKGAFYVNQYFNTLNPEAHYMTT
                     GPEIWRDTEGQIDAVVCGIGTGGTITGIGRYLKDMKKSVRVIGVEPRGSVFKDFLEKR
                     MLVEAGDFHIEGIGKNFIPGVIDFNYIDDVIQVNEDEALRTVEGLVKSEGILAGDSSG
                     AVMAGAIEYARSRAGRERIVVILPDSGLKYLSKFTAFL"
     gene            complement(1959..3344)
                     /locus_tag="VFJ67_04460"
     CDS             complement(1959..3344)
                     /locus_tag="VFJ67_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7289671.1"
                     /translation="MAKERFILGWESGGGSDYLTSALSGRAREVMESPHGMWNYMSYR
                     DAIGILGLDAGEIYEEGRIDLDPRTWADFGWMTCFAGPPESAIEAMRAATTRENVNPY
                     SPDLINPLRDAAAEIKFGRPRDGSFEVIGVEGSQAAISYTLQTFVGPGDEVIITDPGY
                     FHFTSSILMAGGVPVTIPLGPENGFRMRPDEVEKHITPRTKAIIVCDPLNPFGTVQKK
                     DELLALIEIARAKGIIIINDITHGTHRVSPQAVHVPMSSLGGDTNTDNVVSTFSVSHG
                     YGMAGVRIGFLAGHPDVMRACLVTKVALTRINTNLIAQLGALAAIKDTAYVAESETLI
                     RRNCALVKKIVGNNEGLSIPAGPEYGFSLVIDVAGAGVTAQELTVALFKRRVAVYPGD
                     GLGDVGATSYIRLNISRPDVWAFTHLSEALPSAISEARGGAYREAVIKFFESKDNGRA
                     RKIVRMIKGEG"
     gene            complement(3370..4218)
                     /locus_tag="VFJ67_04465"
     CDS             complement(3370..4218)
                     /locus_tag="VFJ67_04465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HET7289672.1"
                     /translation="MSRNGMPGFEELLKPLPWYSPKSWYYGLCSLFLGTVGQLSDGIR
                     TGYRYGFDSGMIMNYIYENKPGGKFYLGKKIDEAFLKQITCRAFRAVKEIQKNMILSY
                     LAEREGMETFIADMASGEADYLYDVLRQTGPLVKAHLRDISRDALEESGKTAARRRLT
                     DRVTFGMGDALDPDSLRVIDGRPDLVIEAGLYGIIHDDALIRAHLAQLRDILNPAALL
                     FNVQTYNPQIELIARALRNQEGGRCVWHLRPAEEVIGWAEEAGFRDARVTYDPYGIYA
                     VVMMRN"
     gene            complement(4208..>4763)
                     /locus_tag="VFJ67_04470"
     CDS             complement(4208..>4763)
                     /locus_tag="VFJ67_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7289673.1"
                     /translation="DPERLRKVLSDPKRLARAFSRHTYGMLAWVDLFGAKLKSIRDPE
                     VKLVAAGIIADNARHAKLFSRRARELGETPETYKPPQIGQEIYDILESYTDTFDELAY
                     AWGSLVHFSALLDIYYSASDEKSRAVIEEVRKDVTRHLKELQGYFEKNADTPEKMKRA
                     EEIKSVADRIYADREDVEIEWYES"
BASE COUNT          837 a         1523 c         1288 g         1115 t
ORIGIN      
        1 ccgcgtattc tatgtcctgg aacctccaga acgaagtcgg cagaatgttt ataccggcgt
       61 cggctacagc ggccctcacg cctccacgga gctccttcat ggtggctacg gtcgtaatca
      121 gatgcacggc gtcgctcact atcgccctcc ccggctcgag aatgaggagc ggcggctctt
      181 ccccgccgca ggccgcgagt ataggttccg tcaccgccga aacatagggc gcgatagagc
      241 ccacgttcgg gaaaccgcct cccatgtcga tgtatttcat ccggacgccg aatttctccc
      301 gcgcctcggt cgcaaagcgc gcagtctcgg acgcggcctt cctgtaagcg cgcgcatcct
      361 tgggccaaat cagctttatg tattcagcgg gcatgccgcc ttcgtcgtgc ggctcgatta
      421 tgtagctcgt caggtggacg tgcagtcctg caatgcggag gagtccgccg gagcaggcac
      481 gctctaccgc acgcatcgca tcgcccgatt cgatgctgaa tccgaacctg tcggggagct
      541 ggtctatgcc cgtctccatg ttgaccctta ttccgatctc gacgtggccg ccttcctcgc
      601 gggctatctc ttcgagcaca tccagctcat cggtatggtc ggcgtttatt atcgcgcctt
      661 ctctcagggc ctccttcagc tcttcctttt tcttatacgg cccgttcagc acgatcgagc
      721 ttcccggtac gcccatccta cgcgcgagcc cgtactcgaa cccggacgcg gcctcggccc
      781 acaagccctc attatgaatg ataccaagca gtgcgggtac ggagttcacc ttgtatgagt
      841 acgcgacctc gacgttcgga taagcggctg agaacgcggc cctgaacgcg cggcagttgc
      901 gaattatggc agatgcggat gcgacgaaaa gcggcgagcc gaattcccgc gcgagatcgt
      961 ttatctgaac gccgtctatt ttatccatgt atgagaccct gcggcaatat gagtatatca
     1021 gaggaacgcc gtgaatttgg agaggtactt gagaccggaa tcggggagta tgacgacgat
     1081 acgctcgcgg cctgcgcggg aacgcgcgta ttcgatagcc cccgccatca ccgcgccgct
     1141 cgaatctccg gcgagaatgc cctcactctt cacgaggcct tcgacggtcc tgagagcttc
     1201 gtcctcgtta acctgtatca cgtcgtctat gtagttgaag tcaatcacgc cgggtatgaa
     1261 gttcttcccg atgccttcga tatggaaatc cccggcctcg acgagcatcc gcttctcgag
     1321 aaagtccttg aatacggagc cgcgcggctc gacgccgatc accctcacgc tctttttcat
     1381 atccttgagg tacctgccta tgcccgtgat cgtccccccc gtccctatgc cgcagacgac
     1441 agcgtcgatc tgcccttcag tgtccctcca aatttcaggg ccggtcgtca tgtaatgggc
     1501 ctcggggttg agcgtgttga aatactggtt cacgtaaaag gcccccttct ccctcgctat
     1561 cctctgggcc accttgtagc agctcctctc gtctgagggc ggcagtcccc tcggcgtgag
     1621 tacgacctcc gcgccgaaga gctttatcgc gagtatcttc tccttgctta tagtctcggg
     1681 cagcgtgaat atgcacctgt aatccctcac cgcgcccacg agggcgaacg cgacgcccat
     1741 gttgccggac gtagcctcta cgatgagatc ccccggttta atgagccctc tcgcctctgc
     1801 gtcgtcgatt atgtatttcg cgactctgtc cttgaagctc ccgctcgggt tcgtgaactc
     1861 gagcttcgca tatatttcgc ttccgaggcc gcctgtgagc ttgttcacgc ggacgagcgg
     1921 cgtgttgcct atgaggctga gcacgtcttc ggataccttc atccctctcc ctttatcata
     1981 cgcacgattt tcctcgcgcg gccgttatcc ttgctctcga aaaattttat gaccgcctcc
     2041 ctgtacgcgc cgccgcgcgc ctccgatata gcggagggga gagcttccga gaggtgcgta
     2101 aacgcccaga cgtcgggcct cgatatgttg agcctgatat aggaggtcgc gcctacgtcc
     2161 ccgagcccgt cgcccggata gacagcgacg cgcctcttga aaagcgcgac cgtgagctcc
     2221 tgcgccgtca cgcccgcgcc tgctacgtct atcacgaggg agaacccgta ctcgggcccg
     2281 gccggaatgg acaggccctc gttatttccg actatctttt tcacgagcgc gcagttcctt
     2341 cttataagcg tctccgattc agcgacgtac gcggtgtctt taattgcggc gagcgccccg
     2401 agctgggcaa tgaggttcgt gttgatgcgg gtaagcgcga ccttcgtcac gaggcacgcc
     2461 ctcatcacgt ccgggtggcc ggcgaggaac ccgatcctca ctcccgccat gccgtaaccg
     2521 tgcgagacgc tgaaagtaga gacgacgttg tccgtattcg tatcgccgcc gagcgagctc
     2581 atcgggacgt gcaccgcctg cggcgagacc ctgtgcgtcc cgtgcgttat gtcgtttatt
     2641 attatgattc ccttcgcccg cgctatctct atcagcgcaa ggagctcgtc ctttttctgc
     2701 actgtgccga aagggttgag cgggtcgcat acgattatgg ctttcgtacg cggcgtgatg
     2761 tgcttctcga cttcgtcagg cctcatgcgg aagccgtttt ccgggccgag cggtatcgtc
     2821 accggcacgc cgcccgccat gagaatcgag gacgtgaagt ggaaatatcc ggggtcggtg
     2881 atgatgacct cgtcgcccgg accgacgaac gtctgaagtg tgtaagagat cgcggcctgg
     2941 ctgccttcga cgcctattac ctcgaatgag ccgtccctcg ggcgaccgaa ttttatttcg
     3001 gccgcggcgt ccctgagggg atttatgagg tcgggcgagt agggattgac gttctcgcgc
     3061 gtcgtggccg cgcgcatcgc ctcgatcgcg ctttccggag gccccgcgaa gcaggtcatc
     3121 catccgaagt cggcccacgt ccgggggtct aagtctatgc gtccctcttc gtatatctcc
     3181 cccgcgtcaa gcccgagtat gccaatagca tccctgtacg acatgtagtt ccacataccg
     3241 tgcgggctct ccataacctc cctcgccctc cccgagagtg cggatgtgag gtaatcggat
     3301 ccgccgccgc tttcccatcc tagtatgaac ctttcctttg ccattattcc cgcgggtcct
     3361 ttcctttatt caatttctca tcattacgac agcgtagatg ccgtatgggt cgtatgtgac
     3421 cctcgcgtcc ctgaaccccg cctcctccgc ccacccgatg acctcctcgg ccggacgcag
     3481 gtgccagacg caccttccgc cctcctggtt cctgagcgcc ctcgcgatga gctcgatctg
     3541 cgggttgtag gtctgcacgt tgaaaaggag cgcggccggg ttaagtatgt ctctcagctg
     3601 cgcgagatgg gcgcgtatga gagcgtcgtc gtggattatc ccgtagagcc ccgcttctat
     3661 cacgagatcg ggcctgccgt ctatgaccct gagactgtcg gggtcgagcg cgtcgcccat
     3721 tccgaaagtt accctgtccg taagcctgcg acgggcggcc gtcttcccgc tctcttcaag
     3781 cgcgtccctg cttatgtccc tcagatgcgc cttcaccagt gggcccgtct gacggagcac
     3841 gtcgtagagg tagtcggcct cacccgaagc catgtccgcg atgaaagtct ccatgccttc
     3901 cctctccgcg aggtagctca atatcatgtt cttctgtatc tctttcaccg ccctgaatgc
     3961 cctgcacgtt atctgtttga gaaaagcctc gtcgatcttt tttcccaaat aaaatttccc
     4021 gcccggcttg ttttcgtata tgtagttcat gatcatcccg gaatcgaatc cgtacctgta
     4081 gcccgtcctt atgccgtcgg acaattgacc cacggtcccg aggaagaggg aacagaggcc
     4141 gtagtaccag ctcttaggcg aataccaggg gagcggcttc agcagctctt caaaaccggg
     4201 catcccgtta cgactcatac cactctatct ccacgtcttc cctgtcggcg taaatcctgt
     4261 cggcgacgga ttttatctcc tcagccctct tcatcttctc gggcgtatcg gcgtttttct
     4321 cgaagtagcc ctgtagctcc ttcaaatgcc tcgtaacgtc tttcctcacc tcttctatca
     4381 cggcacggct cttttcgtca gaggcgctgt agtagatgtc gaggagggcc gagaaatgga
     4441 cgagcgagcc ccaggcgtat gcgagctcgt cgaacgtatc cgtatatgat tcgagtatgt
     4501 cgtaaatctc ctgcccgatt tgaggcggtt tgtacgtttc gggcgtctct cccagctccc
     4561 gagccctccg ggagaagagc ttcgcgtgcc tcgcgttgtc ggcgatgatc cccgccgcga
     4621 ctagctttac ctcggggtct ctgatggatt tcagcttcgc cccgaagagg tccacccacg
     4681 cgagcatccc gtacgtatgc ctgctgaacg ccctcgcgag acgcttcggg tcggacagca
     4741 cctttctcaa cctttcaggg tcg
//
LOCUS       DASSJH010000208         3617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_912696, whole genome shotgun sequence.
ACCESSION   DASSJH010000208 DASSJH010000000
VERSION     DASSJH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912696"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1566
                     /locus_tag="VFJ67_04475"
     CDS             259..1566
                     /locus_tag="VFJ67_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289674.1"
                     /translation="MGKYPPGLDVAVDYKFMDTIFERRSRRFGLGMEIEKGPLAYKSR
                     HAPVPLSELEEAIIVWNGLGIKNINLSDFPPHVGLDLEMQFTSRTIPALGDVHRTELF
                     YTNDDGTYMVKLHDRRPDDFRGLEGMSRDKRVDRILELFRESRIKIEDKRADLPSKPP
                     GLAAHNLWNVNKPGTTLFMPVTDLSACIINLYFFYMRPDHRFNFVDELHGMRAPGTAE
                     WQKKGFLDLGKRMPLIEAELRFANGYIAEQAFMGQNMVLTLQALGLGGWLFSGFASMF
                     MLGGTPFNRGLGFRFITPQLKGETGNPNPVAVGKDGLFQGFCPPYYKDMGEAVEALND
                     MKWTNWESHTMPFKNPEGVLQEVERPSKEEIQVVKDICSYVYDTYGRFPAFSDPMFLR
                     FMVQAHHLDLDFYDKFYPPGAYTENHKNHFKLWHPEITDPFEK"
     gene            1637..2614
                     /locus_tag="VFJ67_04480"
     CDS             1637..2614
                     /locus_tag="VFJ67_04480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HET7289675.1"
                     /translation="MEKIYVSAHYPGEPFERLRENYHVEEFLGTRLPTSDELLEGARG
                     SIAVISTVSDGIDKEFIDLLPGLRIVSNCGVGYENIDVPYATLRGVMVTNTPDVLTET
                     TADLAWALLISAARRVVEADDYVRRGDFKCWHPSLLLGQNIHGKTLGIFGMGRIGTAA
                     ARRASGFGMRVIYNNRKRNERAEIETKAVYVDFPELLKESDFIIVASPLNKGTRGRFG
                     MNEFRQMKRTAVIVNVGRGPIIKEKELAEALKEGVIWGAGLDVYEKEPEVDAGLLRLR
                     NTVLLPHIGSASFETRVRMIEMAVESVELALSGKTPKHLVNPEVLEQIG"
     gene            complement(2670..3158)
                     /locus_tag="VFJ67_04485"
     CDS             complement(2670..3158)
                     /locus_tag="VFJ67_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289676.1"
                     /translation="MIDNYDDFDSDIPVNYRGFDLSEIDYNELPALMEKIDTIHEDMN
                     RIKNLSNKLIGIYESSEPIKGDVSADQVSVYQKFINEGIKEYGEDRGRELIELLLGGP
                     HESPKQQIAKAKALLISYERLFEDEESEPIPPSQFEESDIFKTIKKLTGNKNLFPSLN
                     SQ"
     gene            complement(3280..3516)
                     /locus_tag="VFJ67_04490"
     CDS             complement(3280..3516)
                     /locus_tag="VFJ67_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289677.1"
                     /translation="MKVVSPAGDFEIKVSDSSVEGDYVVLSGQMGVWDSKIYLTPADI
                     WQFAAIFLRPAVILYVLKMPFRSLFGGPEGSSGK"
BASE COUNT          862 a          936 c          991 g          828 t
ORIGIN      
        1 gggggatgtt gtacgaggga taacatgagg cgatcagggc aatgatcagg gctaaaccgg
       61 ctggggaaaa cggagcaagt cgggggctat atttcatatt caggcctttc tgagatcgag
      121 attgaacccg tcatataaaa gtatatatga tgaattattc cgtttcatgt ccgtatcttt
      181 tctattttct cgtccatttg gattacactt tcatttttgc tacaataacc ttcaaaattc
      241 agtgggagat aagtagaaat gggtaaatat ccgccggggc ttgacgtcgc tgtcgattat
      301 aaattcatgg acaccatttt cgagaggagg tcgaggaggt tcggcctcgg gatggagatc
      361 gaaaaagggc cgcttgccta caaatccaga catgccccgg tcccgctctc ggagctcgaa
      421 gaggcgataa tcgtctggaa cgggctcggc ataaagaaca tcaatctctc tgacttcccc
      481 ccgcacgtcg ggctcgatct cgagatgcag tttacgagca ggaccattcc cgcgctcggt
      541 gacgttcaca ggaccgagct tttttacacg aacgacgacg gtacttacat ggtcaagctt
      601 cacgacaggc ggcctgacga tttcagaggg ctcgagggca tgagcaggga caagagggtc
      661 gaccgtatcc tcgagctttt cagggaatcg aggatcaaaa tagaagacaa acgggccgac
      721 ctgccttcca aaccgcccgg actggccgcg cataacctct ggaacgtgaa taagccgggc
      781 acgacgctct ttatgcccgt gaccgacctc tccgcctgca ttataaatct ctatttcttc
      841 tacatgcgtc ccgatcacag gttcaatttc gtggacgagc tccacgggat gagggccccg
      901 ggaacggccg agtggcagaa gaaggggttc ctcgatttgg gtaagaggat gcccctcatc
      961 gaggccgagc tcaggttcgc gaacggctac atagccgagc aggcgttcat gggccagaac
     1021 atggtgctaa cgctccaggc cctcgggctc ggaggatggc tgttcagcgg gttcgcgagc
     1081 atgttcatgt tgggcggcac tccgttcaac aggggactgg ggttcaggtt catcactccc
     1141 cagctcaagg gcgagacggg caaccccaac cctgtggctg tcgggaagga cgggcttttc
     1201 caggggttct gcccacccta ttacaaggac atgggcgagg ccgttgaagc attgaacgat
     1261 atgaagtgga ctaactggga atcccatacg atgcctttca agaatcccga aggagtgctc
     1321 caggaagtag agaggccgag caaggaagag atacaggtcg tcaaggacat ctgcagctac
     1381 gtctacgata cgtacggcag gttcccggcg ttctctgacc cgatgtttct acggttcatg
     1441 gtgcaggcgc accacctcga cctcgacttc tatgacaaat tctatccgcc gggcgcttat
     1501 acggagaacc acaagaacca tttcaagctg tggcacccgg agataaccga cccgtttgag
     1561 aaatagaggg cttacagagc cggattacta aactgcttac ccgattctaa acgggccaga
     1621 accgttcata aatgaaatgg aaaagatata tgtcagcgca cattatccgg gggagccttt
     1681 cgagcggctc agggaaaact accacgtcga ggaatttctc ggcacccggc tgcctacgag
     1741 tgacgagctg ctggagggcg cacgaggctc catagccgta atctctactg tttccgacgg
     1801 gatagataaa gagttcatag accttcttcc gggcctcagg atagtgtcga actgcggagt
     1861 ggggtacgag aacatcgacg ttccttacgc gaccctgagg ggcgtgatgg tcacgaacac
     1921 gcccgacgtc ctcaccgaaa ctacggccga cctcgcctgg gcgcttttga tctcggccgc
     1981 ccggagggtg gtcgaagcgg atgactacgt gaggcgcggg gatttcaagt gctggcaccc
     2041 gtcgctcctc ctcggtcaga acatacacgg caagaccctc ggtatcttcg gcatggggcg
     2101 aataggcacg gcagccgcaa gaagggcctc cggattcgga atgagggtca tatacaacaa
     2161 caggaagagg aacgagcgcg ctgagatcga aaccaaggct gtctacgtgg actttcccga
     2221 actcctgaag gagtctgatt ttattatcgt tgcttcaccc ttgaacaagg ggaccagagg
     2281 ccgtttcgga atgaacgagt tcaggcagat gaaacgcacg gcagtaatag tgaacgtcgg
     2341 caggggcccg atcataaagg agaaggagct tgccgaggct cttaaagaag gggtcatctg
     2401 gggcgcgggt ctcgacgtgt acgagaaaga gcccgaagtc gacgccgggc ttcttagatt
     2461 gaggaacacc gtcctcctgc cccacatcgg gagtgcttca ttcgagacga gggtgaggat
     2521 gatagaaatg gccgttgaaa gcgtagagct cgcgctctcc ggaaagacgc cgaagcatct
     2581 ggtcaacccg gaagttctgg agcaaatcgg ttagcctcgc ttaatctacg gctgtccctt
     2641 tcgcatatgc cccctcatat atgcaccagt tactgtgagt tgaggctagg gaaaaggttc
     2701 ttgttcccgg tgagcttctt tatcgtcttg aatatgtcgc tctcctcgaa ctgggaaggc
     2761 ggaatcggct ccgattcttc gtcctcgaag agcctctcgt aggatatgag aagcgccttc
     2821 gccttggcta tctgctgctt cggggattca tgcgggccgc ccaggagcaa ttctatcaac
     2881 tctctgcccc tgtcttcacc gtattccttt atcccttcgt tgatgaattt ctggtagacc
     2941 gaaacctggt ctgcggaaac gtcgcccttg ataggctcgg atgactcgta tatgccgatt
     3001 aatttgtttg agaggttttt tatcctgttc atgtcctcat ggatggtgtc gattttctcc
     3061 atgagggccg gcaattcgtt ataatcaatt tcggagagat cgaagcctcg ataattgacg
     3121 ggaatatcgg aatcaaagtc atcataatta tctatcatcc tgagtccctc ctttaagatt
     3181 tatctattaa aagtctaaga attcctaagc aaagtttcaa gccctatttt gaattctccg
     3241 ctcgccggca gcgcgggccg cccgaacgac gcggccgctt tacttgcccg agctcccttc
     3301 cgggccgccg aataatgacc ggaacggcat cttcagtaca tatagtataa cggccggcct
     3361 gaggaatata gccgcaaatt gccatatgtc cgccggtgtc aggtagattt tcgaatccca
     3421 aacgcccatc tggccgctca gcacgacgta gtcaccttcg acagatgagt cgctgacctt
     3481 gatttcgaaa tcacccgcgg ggcttacaac cttcatcaca cctccgatct tgattaagct
     3541 gactgctata tattttcatt atcccataaa gcccggagag ataccagtag ctatttagtg
     3601 tgtgactata gggttgg
//
LOCUS       DASSJH010000209         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_376717, whole genome shotgun sequence.
ACCESSION   DASSJH010000209 DASSJH010000000
VERSION     DASSJH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376717"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFJ67_04495"
     CDS             complement(<1..510)
                     /locus_tag="VFJ67_04495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7289678.1"
                     /translation="MVKTESTRSFKKKLYLVIISVLFTLLVLEIATRIWLDFIAPEDV
                     SLEYSLYSYLSPDDQRYVRHHYLNYAPNPEYRRGKTSHNSLGFRNEEFPAKKPEGVFR
                     IVLLGGSTTYNIGIDDNDKTWAARMERILRDKYGYKNVEVINAGVGGYNSWETLINLE
                     FRVLDIEPDL"
     gene            712..1821
                     /locus_tag="VFJ67_04500"
     CDS             712..1821
                     /locus_tag="VFJ67_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289679.1"
                     /translation="MNKYPKILVLILLSLVLSLLIAEGVSRMAFDPIDFLKPRRVPDD
                     VLRYRIEPGTGAHDSLGFRNKSFPAGAEIVAIGDSHTYGISARASESWPSALGRMTGK
                     SVYNMSLGGYGPAEYLYLMESEALKLHPELIIVGFYLGNDLMDSFTAVYSVDKWKDMR
                     KPEFDSSPGNGDADDDDFSYGIGDWLAGHSVLYRLVSSSFIGDNLRQARRMKRGEEIV
                     MFEDGKSGISTGFTPGQRLRGLDLGRAEVREGLRLSLMLFDRMNELARKNNVGFIVVI
                     IPTKESVFARYIGGNKALPASDKIDYLLRNEREADSEIRKYFDEHGIRYVDALGPLSL
                     SAGTEQIYPNNFGGHENKNGYGVIAGAVKEYIDGH"
     gene            1886..2515
                     /locus_tag="VFJ67_04505"
     CDS             1886..2515
                     /locus_tag="VFJ67_04505"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7289680.1"
                     /translation="MVRIKICGITNMEDALRAADLGADALGFIFYKGSKRYIEPRDAG
                     QIISSLPPFVSAVGVFVNQGIEEIKESARVSGIDTVQLHGDETPEFCGRLPQRLIKAI
                     RVKDAVNIAEVELYPVQAILFDKHTDEMYGGTGSSFDWGVLKGLEVSKKIILSGGLTP
                     ENVSLAVRLVKPYGVDVSTGVEDAPGKKNHEKMRKFIEAVKDGGETDRA"
     gene            2490..3698
                     /gene="trpB"
                     /locus_tag="VFJ67_04510"
     CDS             2490..3698
                     /gene="trpB"
                     /locus_tag="VFJ67_04510"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7289681.1"
                     /translation="MAARPIVPDELGHFGIFGGRYVSETLMPAILQLEKAYLEIKDDP
                     KFKQELDYYLKEYVGRPTPLYFAESMTKDLGGAKIYLKREDLAHTGAHKINNTIGQAL
                     LASRMGKKRIIAETGAGQHGVATATVAALFDMECEIFMGLEDTRRQALNVTRMKLLGA
                     TVREVAQGTQTLKDAMNEAMRDWITNVRNTFYIIGSVAGPHPYPMMVRDFQSVIGSEA
                     RRQILEKEKRLPDLLIACVGGGSNAMGLFYPFLGDETVKMTGVEAAGSGIESGHHAAS
                     ISSGTVGVLHGSKTYLLQDENGQVMGTHSVAPGLDYPGVGPEHSYLNDMGRVSYMSVT
                     DTEALEGFKYLSRTEGIIPALESSHAIAYARRIAPGMPKDSVILICLSGRGDKDINAV
                     SDILVRDIKQ"
BASE COUNT          876 a          946 c         1113 g          828 t
ORIGIN      
        1 caggtcgggt tctatgtcga gcaccctgaa ctcgaggttg ataagagtct cccacgaatt
       61 gtacccgccg actccagcgt ttatcacctc cacgttctta tacccgtact tgtctctcaa
      121 tatcctctcc atccgggcgg cccacgtctt gtcgttgtcg tcgatgccga tgttgtatgt
      181 ggtggatccg ccgaggagca ctattctgaa aacgccttcc ggttttttag ccgggaattc
      241 ctcgttcctg aagccgagcg agttatgcga agtcttcccg cggcggtatt cggggttcgg
      301 ggcgtagttc agatagtggt ggcgtacgta tctctggtca tcagggctca ggtacgaata
      361 gagcgagtat tcgaggctta cgtcttcagg ggcgatgaaa tcgagccaga tgcgcgtggc
      421 gatttccagt acgagcagcg tgaagaggac gctgattatt accagataca gctttttctt
      481 gaaagagcgt gtgctttcgg ttttaaccat accttgtccc ggattattgt ttatgcgccg
      541 ggttcgtcaa gtcacccggt tttttcgcat gaggcatcgg taaatatgat agcataggat
      601 ttatcggttt ttctcgtcct tccgccgatt gtctcgcctt tgttcctcga ttatattact
      661 ggaaattcag gatacataaa tgcctcgaac gcgccgggag ttacagccga cttgaataaa
      721 tatcccaaaa tcttggtcct catcctctta tcgctagtct tatcgctcct catagccgag
      781 ggtgtttcga ggatggcttt cgatccgata gatttcctca agccgaggag ggtccccgat
      841 gacgtgctca ggtacaggat cgagcccggc acaggggcac acgactccct cgggttcagg
      901 aacaaatcgt tcccggccgg ggcggagatc gtggccatag gcgactcgca cacttacggc
      961 atcagcgcga gggcgagcga gtcctggccg tccgctctcg ggagaatgac gggaaagtcg
     1021 gtatataaca tgtcgctcgg gggttacggc cctgccgagt acctttacct catggagagc
     1081 gaggcattga agctgcatcc cgagcttata attgtcggat tttatctcgg gaacgacctc
     1141 atggactctt tcacggccgt ttattctgtc gacaaatgga aggatatgag gaagcccgag
     1201 ttcgattcat cgcccgggaa cggggacgcg gacgacgatg acttttcata tggaataggc
     1261 gactggctcg ccgggcacag cgtgctttac aggctcgtga gctcttcctt catcggagat
     1321 aacctgagac aggcgcgcag gatgaaaagg ggtgaagaga tagtgatgtt cgaggacggg
     1381 aagagcggca tcagcacggg atttacaccc ggccagcggc tcaggggact cgacctcggc
     1441 cgcgcggagg tcagggaagg gctcaggctc agcctcatgc tctttgaccg gatgaacgag
     1501 ctcgcgcgta aaaataatgt cgggtttatc gtcgtcatca tacctaccaa ggaaagcgta
     1561 ttcgcccggt acattggtgg taataaagcc cttcccgcct cggataagat agattatttg
     1621 ctgcggaacg agagggaggc tgacagcgag atcaggaaat atttcgatga gcacggcatc
     1681 cgttacgtcg acgcgctcgg ccccctcagc cttagcgcgg gtaccgagca gatttatccc
     1741 aacaacttcg gcgggcacga gaacaagaac ggctacgggg taatagccgg tgcggttaag
     1801 gaatatatcg acggtcattg agatacgtcg ggatccggag cctgttttac ataaactgat
     1861 tgtgatttta taatttccgg ttcatatggt cagaataaag atttgcggta ttacgaatat
     1921 ggaggatgcg cttcgcgcgg ccgacctcgg ggcggatgcg ctgggtttta ttttttacaa
     1981 gggaagcaag aggtatatcg aaccccgcga cgcgggacag atcatttcct ccctgccccc
     2041 gttcgtctcg gctgtcgggg tcttcgttaa ccagggtatc gaggaaataa aagaatcggc
     2101 ccgcgtctcg ggcatagaca cagttcagct tcacggggac gaaacccctg aattctgcgg
     2161 caggctgccc caaaggctga ttaaagcaat cagggtgaaa gatgccgtga acattgcgga
     2221 agttgaactt tatccggttc aggctatcct cttcgataaa catacggatg aaatgtacgg
     2281 ggggactggt tcgagcttcg actggggggt gttaaagggt ctcgaagtat cgaagaaaat
     2341 catactgtcc ggcgggttga ctcccgagaa cgtatccctg gctgtgaggt tagtgaagcc
     2401 ctacggggtt gacgtgagca cgggggttga ggacgcgccc ggaaagaaga accacgagaa
     2461 gatgagaaaa tttatagagg cggtgaaaga tggcggcgag accgatcgtg cctgacgagt
     2521 tggggcattt cgggatattc ggaggcaggt atgtctcgga aacgcttatg cctgctatat
     2581 tgcagctcga gaaagcgtat ctcgagatca aggacgatcc caagttcaag caggagctcg
     2641 actactattt aaaggaatac gtcggacgcc cgacgcctct ttacttcgcc gagagcatga
     2701 cgaaagacct cgggggcgca aagatatatc tcaagcgcga ggacctcgca cacacgggcg
     2761 ctcacaagat aaataacacg ataggtcagg ccttgcttgc gtcgcgcatg ggcaagaaaa
     2821 ggataatcgc cgagacaggc gcgggacagc acggtgttgc gaccgccact gtcgccgctc
     2881 tcttcgacat ggaatgcgag atattcatgg gactcgaaga cacgagacgg caggcactga
     2941 acgtcacgcg catgaagctc ctgggcgcca ctgtgaggga ggttgcccag ggcacccaga
     3001 cgctgaaaga cgcgatgaac gaggcgatga gggactggat aaccaacgtc aggaacacct
     3061 tttacataat cggcagcgta gcggggccgc atccctaccc catgatggtc agagacttcc
     3121 agtccgtcat cggaagcgag gcgaggcggc agatattgga aaaggagaaa cgcctgcccg
     3181 acctgctgat agcgtgcgtc ggcggagggt caaacgcaat gggcctcttc tatcccttcc
     3241 tcggtgatga aacggttaaa atgactggag tggaagccgc ggggagcggt atagagtcag
     3301 gccaccacgc tgcgagcatc tcgagcggaa cggtcggtgt gctccacggc agcaagacat
     3361 atctccttca ggacgagaac ggccaggtca tggggaccca ttccgtagcc cccgggctag
     3421 attatccggg agtgggcccc gaacactcct acctgaacga tatgggaagg gtttcttata
     3481 tgagcgtcac cgatacggag gcgctcgagg ggttcaagta cctttcccgg acagagggga
     3541 taataccggc gctcgaatcc tcccacgcga ttgcctatgc caggaggatc gccccgggga
     3601 tgccgaagga ctcggtaatt ttaatatgcc tctccgggcg gggcgacaag gacataaacg
     3661 ccgtgtcgga tattctcgta agggacatta aacagtaagt tatttcactc gcgcagcgtc
     3721 cgccggcatg ctcgatcggt tgatatttca gccggttcat ccc
//
LOCUS       DASSJH010000210         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_589266, whole genome shotgun sequence.
ACCESSION   DASSJH010000210 DASSJH010000000
VERSION     DASSJH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589266"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFJ67_04515"
     CDS             complement(<1..296)
                     /locus_tag="VFJ67_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET7289682.1"
                     /translation="MLSGYIPVLLIIILAIGLAAAMLGLSAALGPKRDSKRKLSPYES
                     GIPPTGDTRGKFSIKYYLVGALFILFDVEAVFLFAWAVVFKKLGMLAFIEIIVFL"
     gene            434..1249
                     /locus_tag="VFJ67_04520"
     CDS             434..1249
                     /locus_tag="VFJ67_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289683.1"
                     /translation="MSKILTLSVLALLLTYNAYADVVFEMQQKELNPESVGQIKGKVK
                     GNNLRMDFYENGTELEGSMVFRGDKNEIIMINHDNKTYIIMDEAAMNSIAKQLEQAMS
                     QMEAAMKDMPPDQREKMKEMMKQKMPGSGGPYVEPVLKKAGEGKVNGVSCAKYDVYKG
                     DEKVRQHCVADWNSIEGGQEMMTVMLRMADFMDQLTKSFSKAGSLVGDQVQFEKNVFN
                     QLRQLNGFPIETIEYDDGKEDAESTFQSSEKTTVDAADMEPPAGYTKQSTTTN"
     gene            1300..1665
                     /locus_tag="VFJ67_04525"
     CDS             1300..1665
                     /locus_tag="VFJ67_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289684.1"
                     /translation="MIFRLKLSAGFVFTVLAGFAFVSAEHARAEKTACDFQNRECFTE
                     AGYPACYNKIDIQKYYEFSEGGQTALAEEILSDDTRCLKLNGNEKASMMDKSQGFVKF
                     VMRGSNKTLWAKREALYSN"
     gene            1814..2542
                     /locus_tag="VFJ67_04530"
     CDS             1814..2542
                     /locus_tag="VFJ67_04530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7289685.1"
                     /translation="MIVRLNKYISMCGVASRRKADELIETGGVKVNGRVVKELGRTID
                     TETDRIEVLGNVLEPELKRYVMLYKPKLYITALGEGQDKKRTIEELIKSIPERVFPVG
                     RLDYDAEGLIILTNDGELANKILHPKYELPKVYRARVRGVLGTETYTKMKAGTDLEDG
                     PAKPDSIKIIKKDGESTTVELSFHEGRNHLVKRFFAKFGHPVMQLTRMSVGPIKIGSL
                     ERGRWRDLTASELKTLNRALEKAG"
BASE COUNT          753 a          566 c          752 g          531 t
ORIGIN      
        1 agaaaaacta ttatttcaat gaatgccagc attcctagct ttttaaaaac aacagcccag
       61 gcgaacagga atacggcctc cacgtcgaac aatatgaaaa gcgctccgac gagatagtat
      121 tttatagaaa atttgccccg cgtgtctccc gtaggcggga ttcccgactc gtacggggat
      181 agcttccttt tggaatcgcg tttcgggccg agagccgccg aaagccccag catcgcggcc
      241 gcaagcccta tcgcgaggat tattattaat agtacgggga tataaccgct gagcactgaa
      301 ttagattaag ttgtactggg aaattgtcaa gaattttcga ggccggctga ggttaatata
      361 cgagggcttt tgcccgggat gaatgtcggg tcatatatgt tagaataatt ttaaaaattg
      421 aaggaggtcg cagatgtcga aaatactgac tctatcggtg cttgcactgc tcctgactta
      481 taacgcctac gccgacgtcg tgttcgaaat gcagcagaag gagcttaatc ctgaatctgt
      541 cggacagata aagggcaagg tcaagggcaa caacctgaga atggatttct atgaaaacgg
      601 gacggagctc gaaggctcga tggtattccg cggggacaag aacgaaataa taatgatcaa
      661 ccacgacaat aaaacttata taatcatgga cgaagccgct atgaactcga ttgctaaaca
      721 gctggagcaa gcgatgagcc agatggaggc cgccatgaag gacatgcctc ccgaccagag
      781 ggaaaagatg aaggagatga tgaagcagaa gatgccgggc tcgggcggac catacgtcga
      841 gcctgtattg aagaaggccg gtgaggggaa ggtgaacggc gtttcgtgtg caaaatatga
      901 cgtatacaag ggtgatgaaa aagtgaggca gcactgtgtc gccgactgga acagcataga
      961 aggcggacag gagatgatga cggttatgct ccgcatggct gattttatgg atcagttgac
     1021 caaatcgttt tcaaaagccg gaagcctggt cggcgatcag gtacagttcg agaagaacgt
     1081 atttaaccag ttaagacagc ttaacgggtt tcctatagag acgatagaat atgacgacgg
     1141 gaaggaggat gccgagtcca cgttccaatc gtccgagaag acgaccgttg acgcggccga
     1201 tatggagcct ccggcggggt ataccaagca gagcacgact acgaattgaa atcgcatggg
     1261 cttaataaaa accaccgtgt cataaaggag ataattatcg tgatattcag actgaaatta
     1321 tcagccggct tcgtattcac agttctcgcg ggcttcgcat ttgtctccgc ggaacacgcc
     1381 cgcgccgaaa agacagcctg tgactttcag aacagggaat gtttcacgga ggcgggttat
     1441 ccggcctgtt acaataagat cgatattcag aagtactacg aattctccga ggggggacag
     1501 acggcgctgg ccgaggagat tctctccgac gatacccgct gcctgaagct caacgggaac
     1561 gagaaggcct cgatgatgga taagagtcag gggttcgtca agtttgtaat gagggggagc
     1621 aacaagacgc tctgggcaaa acgggaggcc ctttactcga attgagggga tacgcttttc
     1681 tggttttgaa tcaagccggt ccggggttac gtccgaacca caaatatcaa ccggaaaaca
     1741 taacggctat attttttttc cgtgtgcgta aatccgcctt gcaggttaag agggtttccg
     1801 ggagatatgg aaattgatag tcaggctgaa taaatacatc tcaatgtgcg gggtcgcgtc
     1861 taggaggaag gcggatgagc tcatagagac cggcggagtc aaggtaaacg gcagggtagt
     1921 gaaggagctc gggcggacga tcgataccga aaccgacagg atcgaggttt tgggtaacgt
     1981 gctcgagccg gaattaaaaa gatacgtaat gctttacaag ccgaagctct acataacggc
     2041 gctcggcgag gggcaggaca agaagagaac aatagaggag cttataaaga gcatacctga
     2101 gagggtgttt ccggtcggac ggctcgacta cgacgcggaa ggcctgataa tactgactaa
     2161 tgacggcgaa ctcgccaaca agatactgca tcccaagtac gaactcccca aggtttacag
     2221 ggcgagggtc aggggcgtac tcggcaccga gacttatacg aaaatgaagg cgggcacgga
     2281 cctcgaagac ggacccgcaa agcccgactc gataaagata atcaagaaag acggggaatc
     2341 gacgacagtt gagctctcgt ttcacgaggg gaggaaccac cttgtgaaac ggttcttcgc
     2401 gaaattcggc catcccgtga tgcagctcac gcggatgtct gtaggcccga tcaagatagg
     2461 gagcctggag aggggcaggt ggagggacct gacggcatca gagctcaaaa cgctcaaccg
     2521 cgccctcgag aaggcgggct gaagccggcc tgtatcaatt atcgatatta tggtaggatt
     2581 ctgcggtgaa ttaaaaatca aa
//
LOCUS       DASSJH010000211         7688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_269232, whole genome shotgun sequence.
ACCESSION   DASSJH010000211 DASSJH010000000
VERSION     DASSJH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269232"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFJ67_04535"
     CDS             complement(<1..475)
                     /locus_tag="VFJ67_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289686.1"
                     /translation="MKKTYHSVQRRMIMSIRLYGLFHGKNIAVFIISLLLISSVLATQ
                     DSNAQDSLCVGGASNGAIESGEECDDGNEADADGCTGCRLDCEITVEKDAVHADNTQF
                     SFLRLTLPLLRLPIFNQFTLTDPGSNENTFFVPFLSGTFIGEIPAQGWELNGVICD"
     gene            656..1711
                     /gene="ald"
                     /locus_tag="VFJ67_04540"
     CDS             656..1711
                     /gene="ald"
                     /locus_tag="VFJ67_04540"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7289687.1"
                     /translation="MLIGVPKERKIQEYRVALTPEAVRVLAGRGHRVFVEKDAGSGSG
                     IPDGEYKKAGARIVPTEEELFKKAELIVKVKEPLPVEYPLIKEHHTLFCYLHLAADDK
                     LVRALKKSGCRAIAYETVELPDGSLPLLAPMSKIAGRLSVQVGVHFLQKSKGGKGVLL
                     SGAAGVRNGRVTVIGGGTVGLNAVLSALGLGAEVTVIDNKHPKLEFYYEHFEGRVRTL
                     PSYPEIIAGELARSDLAVGAVLVTGARAPRVITKEMIAGMEEGSVFVDVAIDQGGCSE
                     MSVPTTHDAPVYKTLGVTHYCVTNMPALVSRTSTYSLSNTILPYVSALAGGEIDRNEA
                     LLKGINIDDGELKIDIS"
     gene            1933..3612
                     /locus_tag="VFJ67_04545"
     CDS             1933..3612
                     /locus_tag="VFJ67_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7289688.1"
                     /translation="MTMSRNTPVKLFIALFLFFVTGSAAHFSYAGEDYVTDELIVKMK
                     SGTDESEAVAHVRSRGAVVRKSISDLRMMLIQVAPGDTLEDMQRSLENNPDVEYVEKN
                     YIVYADLTPNDPRFGQQSYLNVIDAPPAWNTVTGNSNVVMAILDTGVESTHEDISGKL
                     LPGCNVIGSFTESSCGSNFDDNNGHGTGVAGTAAAKTNNGLGVAGVCWNCQILPVKVL
                     GDSGSGTSVDTVEGIMFARQYALNNPSKRVVINMSLGRDCQASGITQSEQDAINAAWS
                     AGLLVVASAGNSGNSNLQCPAAADHVIAVSATTNSDTLASFSSYGSFVDLAAPGVNIV
                     NAVGTSNVAGSFYGTWSGTSFSAPIVSGVSGLIWSANISLTNAQVDQILRDTAENIGS
                     STFFGDGRVNADFAVDLASSGSPAPTPAPTAPPGPAPTPTPAPVTGGTLTGFVPGTAG
                     VKNSLTVTGAAKGAVVRFYYSRYTGTTSITTGVCKGKTLSLRSPTLLGSATADSTGKA
                     TINATLSSSLGGRTIYIQSRVETTSSCKITNRVTQTIKRSGSSTSRPIRFR"
     gene            3803..4645
                     /locus_tag="VFJ67_04550"
     CDS             3803..4645
                     /locus_tag="VFJ67_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET7289689.1"
                     /translation="MTKILAVLILTLSLLFVVNCGSTKIPKDFTDEQLYDAAMKELTE
                     AKGGFPWIFRGRDYDMVFAYLKEIQLRYTYSPYAALAELRTGDAYFQKGEYEQAAIEY
                     EEFLKRHPGHAEAPYATYRLALSYYKEIKSPDRDPLNTRTALQWFNTFIEKYPDSPLV
                     PDARARAMRCRDRLARREIYIGNFYSGRDNYKAAADRYKIVVEDYNDTQRYQEALYLL
                     GKAYAKSDQYDLARQTLTRLVQEFPNEKYSGKATSLLNDIQGKTAPPPQEEKKAEPAE
                     PQPG"
     gene            4729..5100
                     /locus_tag="VFJ67_04555"
     CDS             4729..5100
                     /locus_tag="VFJ67_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289690.1"
                     /translation="MSIRAKAFETKDADLYMTLIAPDYKQEKKGKTIGPGEVKKNFEV
                     NVKLFDTIRLTSTDRTIYTEGDRAEVFQKTIIDATDDQGKSRLRIKEKLSLARENGKW
                     LIVRESDEDFFYGYVFGRSKD"
     gene            complement(5111..5473)
                     /gene="dksA"
                     /locus_tag="VFJ67_04560"
     CDS             complement(5111..5473)
                     /gene="dksA"
                     /locus_tag="VFJ67_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HET7289691.1"
                     /translation="MDKPRLKHFREVLIKKMAGLLGIADDTVTQMSEGDGTFPDPLDR
                     ALSESSRTIELRKRDRERKLIQKIQKAIQKTEDGSYGVCEICGEEITEDRLEVRPETT
                     LCIGCKEEQEEVERQFGG"
     gene            5639..6511
                     /locus_tag="VFJ67_04565"
     CDS             5639..6511
                     /locus_tag="VFJ67_04565"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7289692.1"
                     /translation="MFKLSQIRFRVSEDAVSLYSDFLLGLGSLGVAEDIREDGMYELS
                     AYFPMDTDLSSVMESLREQAGVIKESVAGAKIGPVEVEHIDRSSWEVWKTVLTTVRAG
                     EGVVIVPPWEKYAPAPGETVVEINPSLAFGTGHHETTRLCIAFIEERARSGSVNSMLD
                     VGCGSAILAITAYKLGIGNVTAFDNDTIAISESRKNARRNGVLGKIKFFCGYLQSIRG
                     VYDLIVANVYIEPIYHMREELKSRLAPGGRLVVSGIPLTRRDDAVRYLVGAGYGLYEE
                     KREGDWVALGFRLD"
     gene            6574..7608
                     /gene="mtnA"
                     /locus_tag="VFJ67_04570"
     CDS             6574..7608
                     /gene="mtnA"
                     /locus_tag="VFJ67_04570"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET7289693.1"
                     /translation="MSSFKTIEWKDDRVVMIDQTKLPREESYVECMTYEDVADAIKRM
                     VIRGAPAIGVAAGMGIALGAIKIRTTDRDEFITEVRRIADVILGTRPTAVNLSWAVKR
                     MLALLAEGRGDVEEMKSRLAGEARYMLEEDISICMRIGKAGAPLIKDNSTVLTHCNAG
                     ALATGGYGTALGVIRAAVEEGKKIRVIATETRPFLQGARLTAWELDRDGIPVSLITDS
                     MAGYIMSKGMVDAVVVGADRIAANGDVANKIGTYTISVLAKTHDIPFYVAAPISTIDF
                     DCPGGRAIPIEERNPYEVTHISGIRIAPEVGVFNPAFDITPSGNVTSIITEKGVVISP
                     FEVSLSKLRG"
BASE COUNT         1844 a         2017 c         2173 g         1654 t
ORIGIN      
        1 agtcgcagat tacgccgttc agctcccatc cctgagccgg aatctctccg atgaatgttc
       61 ccgagagaaa cggcacgaag aaagtgtttt cgtttgagcc gggatcagtc agcgtaaatt
      121 gattgaatat tggcagcctg agcagcggca gtgtcaacct caggaacgaa aactgggtgt
      181 tatcggcgtg aacggcatct ttttccaccg ttatttcaca atccagcctg cagcccgtgc
      241 agccgtccgc gtcggcttcg ttgccgtcgt cacattcttc gccggattca attgctccat
      301 tagacgcacc gccgacacat aacgaatcct gcgcgtttga atcctgagtt gctaaaaccg
      361 atgatatcaa gagaagtgaa atgataaata cagctatatt ctttccgtga aagagtccat
      421 acaatcggat cgacataatc atcctccttt gaaccgaatg gtatgttttc ttcattatac
      481 aaataacatc attatttgta caggctttgt gagcttgaaa tggaacttga cgcattatgt
      541 gtagtattgg ttctgacggc gttgtattgt tgtattaaag attgcaaata ggacacaact
      601 cagacaaact aactactttt caatcactta ttcaaatgct attctgtaat cacccatgct
      661 cataggcgtt cccaaagaga ggaaaattca ggaatacagg gtcgctctca cgcctgaagc
      721 cgtccgcgtt ctcgccgggc gcgggcacag ggtcttcgtc gaaaaagacg cgggctcggg
      781 gagcgggata ccggacggcg aatataagaa agcgggcgcg cgcatcgttc ccaccgaaga
      841 agagcttttc aaaaaagcgg agctcatagt aaaggtaaaa gagcccctcc ccgtcgaata
      901 ccctttgata aaagaacatc atacgctctt ttgctacctc cacctcgcgg ccgatgacaa
      961 attagtccgc gcgctcaaga agagcgggtg cagggcgatt gcgtacgaga cggtcgagct
     1021 tccggacggc tcgctacctc tcctcgctcc gatgagtaag attgcgggca ggctctctgt
     1081 ccaggtgggc gtccatttcc tccagaaatc gaagggaggg aagggcgtgc tcctctcggg
     1141 cgccgccggg gtgaggaacg ggcgggtaac agtcattggc ggcggcactg tggggcttaa
     1201 cgccgtactc tccgcacttg gtctcggcgc ggaggtgacg gtcatagaca acaagcatcc
     1261 gaagctcgaa ttctattacg agcacttcga gggcagggtc aggacgctac cttcttatcc
     1321 cgagataatt gccggggagc tcgcaaggtc cgacctcgcc gtaggcgcgg tgctcgttac
     1381 gggcgcaagg gccccgaggg tgataacgaa ggagatgata gccggtatgg aggaggggag
     1441 cgtcttcgtc gacgtggcca tagaccaggg cgggtgctcc gagatgagcg tcccgaccac
     1501 gcacgacgcc ccggtatata aaactctcgg cgtgacccat tactgcgtga ccaatatgcc
     1561 cgctcttgtg tccaggacct cgacatattc actttcaaac accatacttc cttatgtatc
     1621 ggctcttgcc ggcggggaaa tagatcggaa cgaggcgctg ctcaaaggaa taaatatcga
     1681 cgatggtgag ttgaaaattg atatctcatg aaatttccct gatatcatag gcaagtaaaa
     1741 atggtgacaa tttgcgtcac cctgcaacaa ttatttgttg aatgtctgcc ggctccgatg
     1801 tttaaagtga cttaagcgct tgcatttatt gataaacctg atcaggtgcg ggtggcataa
     1861 actttgcaac ttttaattac ggggttattc aataaatttc atctgttctc gcttacaggg
     1921 aggatttcaa taatgacaat gtcaagaaat acgccggtga aattatttat agccctcttt
     1981 ctctttttcg ttacgggttc cgccgcacat ttttcttatg ccggggaaga ctatgttaca
     2041 gacgagctga tcgtgaaaat gaagagcggc acggacgaat cggaagcagt cgctcacgtt
     2101 agatcgagag gcgctgtggt gagaaagtcg atctccgatc tccgcatgat gctcattcaa
     2161 gtggcgcccg gagatactct cgaagacatg cagaggtcgc tcgaaaataa tcccgacgtc
     2221 gagtacgtcg aaaagaatta catagtatat gcggatttga cccccaacga tccgaggttc
     2281 gggcagcagt catatctgaa cgtcatagac gcgcctccgg catggaacac ggtgacgggc
     2341 aattcgaacg tagtaatggc gatacttgat accggagtgg aatccacgca cgaggacata
     2401 tccgggaaat tacttcccgg ctgcaatgtt atcgggagct ttacggaaag cagctgcgga
     2461 tctaacttcg atgataacaa cggacacggc acgggcgttg cgggcacggc cgcggccaag
     2521 acgaacaacg gcctcggcgt tgccggggta tgctggaact gccagatact tcccgtaaag
     2581 gtgctgggcg acagcgggtc cggcacgtcg gtcgacaccg tcgaaggcat tatgttcgcc
     2641 cgccaatacg ctttaaacaa tccgagcaag agagtggtaa taaatatgag cctgggcagg
     2701 gattgccagg cctcgggaat tacgcagtcc gagcaggacg ccattaacgc cgcatggagc
     2761 gcaggcctcc tcgtggtcgc gtccgccggt aattcgggaa acagcaacct tcaatgccca
     2821 gcggccgcgg accacgtgat agccgtgtcg gcgaccacca acagcgacac gctcgcgagc
     2881 ttctcgagct acgggagctt cgtggatttg gcggcgccgg gtgtgaatat cgtaaatgcc
     2941 gtcgggacgt caaacgtcgc cggaagcttt tacggcacat ggtccgggac atctttttcc
     3001 gcgcccatag taagcggagt ctccgggctt atctggtctg ccaacatctc ccttactaat
     3061 gcccaggtgg atcagatatt gagggatacg gcggagaata taggttcttc tacctttttc
     3121 ggcgacggca gggtgaatgc tgattttgcg gtggacctgg cttcttccgg aagccccgct
     3181 ccaacgcccg ctccgacggc gcctccgggc ccggctccta ctcccacacc tgctccggtc
     3241 acgggcggaa ccctcacggg ctttgtcccc ggaacggccg gcgtcaagaa ttcgctgacc
     3301 gtaaccggcg ccgcaaaggg tgcggtcgtc agattctact attcaaggta tacaggtaca
     3361 acttcaatta cgaccggggt ttgcaaaggg aagacgctga gcctgagaag tcctacttta
     3421 ctaggttcgg ctacagcgga ttctaccgga aaagcgacga tcaatgcgac gttgtcgagc
     3481 agcctgggcg gaaggaccat atacattcag tcaagagtgg agactacctc ttcgtgcaag
     3541 atcacgaaca gggtgactca gaccatcaag agatcggggt catctacgag cagaccgatc
     3601 cggttccggt aatttgaacg attaattccg gccgtcttat gcgggtgctt tgctccgggg
     3661 cgggagccct gtacaatatc cgggtccgcc ggtgcggtaa ggagtgacct gcccggcccc
     3721 tcgctacagc tgatcattcg cattccgcca atttcggtcc gaacttgaca cggatattat
     3781 tatctaaata ctataagact tcatgacgaa aattctcgcg gttctgattc tcacactctc
     3841 actgctcttc gtggtaaatt gcggctcgac caagattccc aaggatttca ccgacgaaca
     3901 gctctacgat gcggcaatga aggagctgac cgaggccaaa ggcggtttcc cgtggatatt
     3961 ccggggcagg gactacgaca tggtctttgc ctacctgaaa gaaatacagc tcaggtacac
     4021 gtacagcccc tatgcggcgc ttgccgagct caggaccggg gacgcgtatt ttcagaaagg
     4081 cgagtacgag caggccgcga tcgagtacga ggagttcctg aagcggcatc ccggccacgc
     4141 cgaggctccc tatgccacgt acaggctcgc tctatcgtat tataaggaga tcaagagccc
     4201 ggacagggac ccgctcaata cgaggacggc ccttcaatgg ttcaatacct ttatagagaa
     4261 atacccggat tccccgctcg tgcccgatgc gcgcgctagg gctatgagat gcagggacag
     4321 gctggcccgg agagagattt atataggcaa tttttattcg ggacgcgata actacaaagc
     4381 agcggccgac aggtacaaga tcgtcgtaga ggactataac gacacacaga ggtaccagga
     4441 agcgctatat ctgctgggca aggcctacgc gaaatcagac cagtacgatc tcgcgcgtca
     4501 gaccctgacc cgcctcgttc aggagttccc caacgaaaag tattccggca aggcgacctc
     4561 tctcctgaac gacatacagg gcaaaacggc cccacccccg caggaagaaa aaaaggcgga
     4621 gcccgcagag cctcagcccg gatgaggccc ctcctctgta tttccattat ctttacgttg
     4681 tttgcagtct cctgcgccgg cgacgatcaa aaggctatcg aggaggttat gtctatcagg
     4741 gcgaaggcct tcgaaacgaa ggacgcggac ctctacatga cgcttatcgc gcccgattac
     4801 aagcaggaga agaaaggcaa gaccataggg cccggggagg ttaaaaagaa tttcgaggta
     4861 aacgtaaagc tcttcgacac catccggctc acgagcacgg acaggaccat atacacggaa
     4921 ggagataggg ccgaggtctt tcagaaaacg atcatcgacg cgaccgacga ccagggaaag
     4981 agcaggctca ggatcaagga gaagctgtcg ctcgccaggg aaaacggcaa gtggcttata
     5041 gtcagggaat ccgacgagga ttttttctac ggttatgtat tcgggcgaag caaggattga
     5101 gcgatacctc ttacccgccg aattgccttt ccacttcttc ctgctcttcc ttacagccta
     5161 tacagagcgt ggtctccggc cttacctcga gcctgtcttc cgtgatctct tccccgcata
     5221 tctcgcatac gccgtaagac ccgtcttcgg ttttctggat agctttctga atcttttgaa
     5281 taagctttct ttccctgtcc cgcttccgga gctctatcgt tctgctggat tcggagagcg
     5341 ccctgtcgag agggtcggga aacgtcccgt ctccctcgct catctgtgtg acggtgtcgt
     5401 cggcgattcc caggagaccg gccatttttt ttataagtac ttccctgaag tgcttcagcc
     5461 ttggcttatc catcggaaaa ttatatcacc tgtgtcaggt agtgcaaaac aaaaaagagc
     5521 tcggcaacag gccggctttc gctcccgcgt acccgcgcgc tcgttccggg attccgcatt
     5581 atataattaa tatttctcgg ttattataat tcactaatct cgatttcaag gggggctgtt
     5641 gtttaagctg tcacagatca ggttcagggt ttcggaggat gccgtctctc tctattcgga
     5701 cttccttctg gggctgggct ctctcggcgt cgccgaagac atcagggagg acggcatgta
     5761 tgagctgtcc gcatatttcc cgatggatac ggacctctcc tcggtgatgg agagcctgag
     5821 agagcaggcg ggtgtaatta aagagagcgt cgcgggagcg aaaatagggc ctgtagaggt
     5881 cgagcacata gaccgctcga gctgggaagt ctggaagacc gtgcttacga cggtcagggc
     5941 gggcgagggg gtcgtcatag ttcctccctg ggagaagtac gcgcccgcgc ccggcgagac
     6001 tgtggtcgag ataaacccgt cgctcgcctt cggtacgggg catcacgaga cgacgcggct
     6061 ctgtatcgcg ttcatcgagg agcgcgcgag gtcgggcagc gtgaacagca tgctcgacgt
     6121 cggatgcggg agcgctattc tcgcgatcac cgcttacaag ctcggcatcg ggaacgtgac
     6181 ggccttcgat aacgacacga tagctataag cgagtcgagg aagaacgcac gccgaaacgg
     6241 cgtgctcggc aagattaaat tcttctgcgg gtacctgcaa agcatccgcg gcgtctacga
     6301 ccttatagtc gcaaacgtct atatcgaacc catctaccac atgagagagg agctgaaatc
     6361 gagactcgct cccggaggaa ggctcgtcgt ctcgggcata cccttaacaa ggagagacga
     6421 cgccgtccga tacctcgtcg gcgccggata cggtctctat gaagagaaga gggagggcga
     6481 ctgggtcgct ctcgggttca ggctcgattg attaagcaat acgctcctgt aaactgatac
     6541 gcgtgtttaa ataagatttt gaagggtaac ggtatgtctt cgtttaaaac catagagtgg
     6601 aaagacgaca gggtagtgat gatagatcag acaaaactgc cccgcgagga atcgtacgtc
     6661 gaatgcatga cctacgagga cgtggccgac gctatcaaac ggatggtcat aaggggcgcc
     6721 ccagcgatcg gcgtcgctgc gggaatgggt atagcgctgg gcgcgattaa aatacggaca
     6781 acggacaggg atgaatttat taccgaggtg cggaggatcg cagacgtgat cctcggaacg
     6841 agacccaccg ccgtgaacct ctcctgggcc gtgaagagga tgctcgctct tctcgcggaa
     6901 gggagagggg acgtggagga gatgaagtcg aggcttgccg gggaagcccg gtacatgctc
     6961 gaagaggata taagcatctg catgaggatc ggaaaagccg gggctccgct cattaaggac
     7021 aattccacgg tgctcacgca ctgtaacgcg ggggctctcg ccaccggagg ctacgggact
     7081 gctctcgggg tcatcagggc ggccgtggaa gagggtaaga agataagagt catcgccacc
     7141 gagacgaggc ccttcctcca gggcgcgagg ctcacggcgt gggagctcga cagggacggc
     7201 attcccgtct cgctcatcac ggacagtatg gcggggtaca taatgagcaa agggatggtc
     7261 gatgccgtcg tcgtaggggc ggacaggatc gcggctaacg gcgacgtcgc caacaagata
     7321 gggacctaca ccatatccgt cctggcgaag acacacgata tacccttcta cgtagcggct
     7381 cccatctcca ccatagattt cgactgcccg ggagggagag ctatcccgat cgaggagagg
     7441 aacccctatg aagtcactca tatatccggc ataaggatcg cgcccgaggt gggtgtcttt
     7501 aatccagcct tcgatattac cccaagcggg aatgtaacct cgattataac cgagaagggc
     7561 gtcgtgataa gcccgtttga ggtcagtctc tcaaaattgc gcggttagcg ggggacacta
     7621 cttcctttcc aggtcttcct ggcaggaaag gcatagtttg gtgaagggca tggccgcgag
     7681 tctttcct
//
LOCUS       DASSJH010000212         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_269577, whole genome shotgun sequence.
ACCESSION   DASSJH010000212 DASSJH010000000
VERSION     DASSJH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269577"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFJ67_04575"
     CDS             complement(<1..899)
                     /locus_tag="VFJ67_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sll0787 family AIR synthase-like protein"
                     /protein_id="HET7289694.1"
                     /translation="MEKELQTLIRTLKSSTRLVQKQYLVGLWRYFPLAPSIDGNDILL
                     GDDAAAIKSGDSFLLLAAEGVYQPLLDSNPYLAGRTSVLTNVNDIYAMGGRPVALVDV
                     LFSKSEASAGEVLRGIRDNAERYGVPVVGGHTTLDAESGSLSVFILGKAERLLSGFNA
                     RPDDDILLAAALEGKFYPAFNFWDSSSGLSESDLLWQLEILPGLAESGLADAARDVSM
                     AGLIGSALMMLESSKSGAEIFIDRIPVPEGVPLQKWLLTFPSYGFVISSRPGKTQYIK
                     DRFRDAGLACETIGRVTDGGRVY"
     gene            complement(907..1410)
                     /locus_tag="VFJ67_04580"
     CDS             complement(907..1410)
                     /locus_tag="VFJ67_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HET7289695.1"
                     /translation="MSSAIHTITATGGERIRKSGEYVFKVAGSPEELESYFRLRHAVF
                     VEEQKIFSGTDVDERDEGAIHIIALKGPDSVMVGGVRCYTTGDDTWYGGRLTAANGYR
                     NGRVGSGLVRFAVETVKSRGCKKFLAYVQPQNVNFFKRLDWKPVGEPVVYEGITHQLM
                     EADLGSA"
     gene            1692..2069
                     /locus_tag="VFJ67_04585"
     CDS             1692..2069
                     /locus_tag="VFJ67_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7289696.1"
                     /translation="MPIYEYHCPGCNKEVSIFFLTISEAETEKPVCPECGSGNLGRLI
                     SNVSAVSKPDNAKNKDIPGKSPAAGEDTASLARVMREAGRKSKKGFGEQFSEVTARLE
                     KGEPSKSIERSLRKRVGEAMETP"
BASE COUNT          441 a          641 c          584 g          428 t
ORIGIN      
        1 aagtaaaccc tgccgccgtc ggtcaccctt cctatcgtct cgcacgcgag tcccgcgtcc
       61 ctgaacctgt cctttatgta ttgtgtcttt ccgggcctcg aggatatgac gaacccgtag
      121 ctcgggaacg tcagcagcca cttctgaagc ggcacgcctt cgggcacggg aatcctgtcg
      181 ataaaaattt cggcgcctga cttcgagctc tcgagcatca tgagcgcaga gcctataagg
      241 cccgccatgc tcacgtccct tgcggcgtcg gccaggcccg actccgcgag cccgggtagt
      301 atctcgagct gccagaggag gtcgctctcg gaaagcccgc tggaggagtc ccagaaatta
      361 aacgccggat agaatttgcc ctccagagcc gcagccagga gaatgtcgtc gtccggacgc
      421 gcattgaacc cggacaggag ccgctccgcc tttccgagta tgaacacgga gagcgagccc
      481 gactcggcgt cgagagtggt atgcccgcca acaacgggaa ccccgtaacg ctcggcgttg
      541 tccctgatgc cgcgcagcac ctcgcccgcg ctcgcttcgc ttttggagaa gagcacgtcc
      601 acgagagcga cgggccttcc gcccatggcg tatatgtcgt tcacgttcgt gagcaccgaa
      661 gtcctgcccg cgaggtacgg gttcgaatcg aggagaggct ggtacacgcc ttccgcggcg
      721 aggagaagga aactatcccc ggattttatg gcggctgcgt cgtcgcccag cagtatgtcg
      781 ttcccgtcta tactgggcgc gagcgggaag tagcgccaca ggcccacgag gtattgcttc
      841 tggacgagcc tggtcgagga tttgagcgta cgtatcagtg tctggagctc tttttccatt
      901 tctctttcat gcggagccga ggtccgcttc catcagctga tgagttattc cctcgtaaac
      961 gaccggctcc cccacgggct tccagtcgag cctcttgaaa aaattgacgt tttgaggctg
     1021 aacgtaagcg agaaattttt tacatcccct cgatttcacc gtctcgaccg cgaacctcac
     1081 gagacccgag cccacgcgtc cgttcctgta accgttagca gccgtgagcc gcccgccgta
     1141 ccacgtgtcg tcgcccgtcg tatagcacct cacccctccc accatcacgc tgtcaggacc
     1201 cttgagcgcg attatgtgta tggctccctc gtcccgctcg tccacgtccg tgccgctgaa
     1261 tatcttctgc tcctcgacga agaccgcgtg acggagcctg aaatagctct cgagctcttc
     1321 cgggctcccg gcgactttaa atacgtattc cccggatttt ctgatccgct ctccccccgt
     1381 cgctgtgatt gtgtgaattg ctgagctcat aacaaccgct ccttcaggta ttccgctggt
     1441 cttccgcgcg tgcgcgctcc cgggcccgtt tcgaatgtga acggcccggc gccaagctgc
     1501 cggaacgtcc actatacttg acactatatc tactttagta aatcaagtca aggaagttcc
     1561 tgggcgcgga cgaggccgta aaaacgatgc ggcggacgcc gcccggacgg gccggtgagg
     1621 gacgctgtct ctccttgact agcactcata aatttcctat aatgtacgaa gcgtacacta
     1681 taggaaaaaa tatgcccatc tatgaatacc actgtcccgg ctgcaacaaa gaagtaagca
     1741 tctttttcct taccatatcg gaagcagaga ccgaaaaacc cgtctgtccc gaatgcggga
     1801 gcggaaatct cggacgcctg atctcgaacg tatcggccgt atcgaagcct gataacgcga
     1861 aaaataagga cataccgggg aagagcccgg cggcagggga agacacggcc tcgctcgccc
     1921 gcgtgatgcg cgaggcgggc cggaagtcga agaaaggttt cggagagcag ttcagcgagg
     1981 taacggcccg tctcgaaaaa ggcgagccgt cgaaatctat tgagaggtcg ctcaggaaaa
     2041 gggtcggcga ggcgatggaa acaccatagc cggcatatga tcagcggtcg gtat
//
LOCUS       DASSJH010000213         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_270304, whole genome shotgun sequence.
ACCESSION   DASSJH010000213 DASSJH010000000
VERSION     DASSJH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270304"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFJ67_04590"
     CDS             complement(<1..226)
                     /locus_tag="VFJ67_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289697.1"
                     /translation="MKKEDIEKIIDSWKTHLLVGPLEGYQLEISDDVPEAFAAVALYL
                     DSKTVRASGEVEDYYEGYRQAAVDVLNLIGV"
     gene            complement(346..759)
                     /locus_tag="VFJ67_04595"
     CDS             complement(346..759)
                     /locus_tag="VFJ67_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET7289698.1"
                     /translation="MVGIVVVTHGELAKELIAAVNFVLSSNPSVKMEGVCLHPDREFE
                     TFKQEIKKAIKKVKEKDGILLVTDMFGGTPSNISLTFLEENNIEVISGVNLPMLLKLA
                     TLSDKVKLAEAVKIAEAAGRDNIIVASKLLHSRNS"
     gene            complement(773..1624)
                     /gene="rapZ"
                     /locus_tag="VFJ67_04600"
     CDS             complement(773..1624)
                     /gene="rapZ"
                     /locus_tag="VFJ67_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET7289699.1"
                     /translation="MTNLVIISGVSGSGKSTAMNVLEDLGYYCVDNLPMTLLPKFIEL
                     CENSQGDINKIALVVDIREGVFFKGAPEVIRELREKGHPIDVIFLDSSDETLVKRYKE
                     TRRKHPLSVNGNILEGIAKEREMLRELKELSSYSIDTSDFNVHDLKELIRNKFDKSRT
                     RNLLVNILSFGFKHGYPYDADMVFDVRFLQNPYFVEELKEMNGLDEEIIQFVMSKEDT
                     KEYIRKLTDFLEFLIPRYEKEGRSYLTIAIGCTGGKHRSVVVARELSEHFKHLSPVTI
                     HRDISKS"
     gene            complement(1655..>2246)
                     /gene="hprK"
                     /locus_tag="VFJ67_04605"
     CDS             complement(1655..>2246)
                     /gene="hprK"
                     /locus_tag="VFJ67_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET7289700.1"
                     /translation="CNKREIPLFTTKLYTGKLITLLQEILEVRLAPFATVHGVLMDIH
                     RLGVLILGKSGIGKSECALDLINRGSKLISDDIVEIRKVGVSRLVGNGPERIKHLMEV
                     RGVGIINIKDLFGTTSVMDKREIDIVIELVHWDPGAEYDRLGIEVSNYTILGVDLPHL
                     VIPVSPGRNTATIIEVAARNQLLKMSGVQTPRNFDT"
BASE COUNT          442 a          627 c          546 g          631 t
ORIGIN      
        1 cgacgccgat caggtttagc acgtccaccg cggcctgcct gtagccctcg taatagtcct
       61 ccacctcgcc cgatgctctc actgtcttcg agtcgaggta tagcgcgacg gcggcaaatg
      121 cctcaggcac gtcgtcagag atctcgagct ggtatccctc gagcgggccg accagcaggt
      181 gggtcttcca tgagtctatg attttttcta tgtcttcctt cttcataact attataaatt
      241 aacccttatt gccgtgcgct tcaagtgccc ggatgtgagt gaaataatcg cctccggatc
      301 atcggaacgg ccgcgggtct gtctgccgac gggcttctcc ctctttcagc tgttcctgct
      361 gtgaaggagc ttgcttgcta cgattatgtt gtccctgccc gccgcctccg ctattttaac
      421 cgcttcagcg agctttacct tgtccgaaag cgttgcgagc ttaagcagca tcggcaggtt
      481 tacgccggag ataacttcga tgttgttttc ttcgaggaac gtcaggctga tgttggatgg
      541 agtcccgccg aacatgtccg tgaccaggag tatcccgtct ttttctttca cctttttgat
      601 cgctttcttt atttcctgct tgaaggtctc gaattccctg tcggggtgga gacagacccc
      661 ttccattttt acggacgggt tcgacgacag cacgaaattt acggccgcaa taagctcctt
      721 ggccagctct ccgtgggtta ctacgacaat acctaccatc ccgaaagtct ccttagctct
      781 ttgatatgtc cctgtgtatc gtgaccggcg acaggtgttt gaaatgctcg gacagctctc
      841 tggccacgac taccgacctg tgcttcccgc ccgtgcaccc gatggcgatc gtaaggtacg
      901 atctcccttc cttctcatac ctcggtatga ggaattcgag gaaatcggtg agcttcctga
      961 tatactcctt cgtgtcctct ttgctcatta caaattgaat gatctcttcg tcgagcccgt
     1021 tcatctcctt gagctcctcg acgaaatacg ggttctgcag gaacctcacg tcgaagacca
     1081 tatccgcgtc ataagggtac ccgtgcttga atccgaaaga caatatgttg acgaggaggt
     1141 tccgcgtgcg cgatttgtcg aatttgtttc tgatcagctc ctttaaatcg tgcacgttga
     1201 aatcggaggt gtctatgctg tagctcgaga gttcttttaa ctcccggagc atttccctct
     1261 ccttcgcgat gccttcgagt atattcccgt tgaccgacag cggatgtttc ctccgagtct
     1321 ccttgtatct ttttacgagc gtttcatccg aggaatcgag gaatatcacg tcgatcgggt
     1381 ggcctttctc cctgagctcc cttatgacct cgggagcccc cttgaagaag actccctcgc
     1441 gtatatccac cacgagggct atcttgttga tgtccccctg ggaattctcg cagagctcga
     1501 tgaacttcgg caggagcgtc atcgggagat tatcgacgca gtaatatccg aggtcctcga
     1561 gaacgttcat ggcggtgctt ttgcccgagc cggaaacgcc gcttataata accagattag
     1621 tcattcgggt ccttgttttg cggcgtgacg ggatttaagt atcgaagttc ctgggcgttt
     1681 ggacgccgct cattttcagt aactgattcc gcgccgcaac ttcaattatg gtagccgtat
     1741 ttctgccggg gctcacgggg attacgagat gcgggagatc gacgccgagt attgtgtaat
     1801 tgctgacttc aattccgagc ctgtcatatt ctgctcccgg gtcccaatgg acaagctcta
     1861 tgacaatatc gatttctctc ttgtccatga ccgatgtcgt gccgaacaga tccttgatat
     1921 taattatacc cacacctctc acttccatca ggtgttttat gcgttccggc ccgttgccga
     1981 caagcctcga tacaccgact ttccttatct ccactatgtc gtctgaaatg agtttagaac
     2041 ctctgtttat taagtcaaga gcgcattcgc tcttgcctat cccgctcttg cccagtatca
     2101 ggaccccgag cctgtggatg tccataagga ccccgtggac ggttgcgaaa ggggcaagcc
     2161 tcacctccag aatttcctgg aggagcgtaa tgagcttccc cgtgtagagc ttcgtcgtaa
     2221 agagagggat ctccctcttg ttgcag
//
LOCUS       DASSJH010000214         4596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_591053, whole genome shotgun sequence.
ACCESSION   DASSJH010000214 DASSJH010000000
VERSION     DASSJH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591053"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFJ67_04610"
     CDS             complement(<1..823)
                     /locus_tag="VFJ67_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7289701.1"
                     /translation="MSKIDNQLRVESLALHGGWEPDPTTGARALPIYQTTSFQFKSTE
                     HAANLFGLREFGNIYTRIMNPTQDAFEKRIAAMDGGVGALAVSSGQSATTIALLNIAQ
                     AGDEIVSMDNLYGGTYNLFHYTFPRLGVIVKFVKSNDLKALQKAITSKTKAVYGESIG
                     NPKLDIADLEGISGVAHKNGIPFVLDYTVSPYLLRPFDFGVDITVYSATKFIGGHGTS
                     IGGVIVDSGKFEWDSGKFPLISEPDPSYHGINFIEALKPLGNIAYIIKARVTLLRD"
     gene            complement(1191..1601)
                     /locus_tag="VFJ67_04615"
     CDS             complement(1191..1601)
                     /locus_tag="VFJ67_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET7289702.1"
                     /translation="MTSKRAVRILIGKVGLDGHDRGVKVISRALRDAGYEVIYTGLHN
                     TPQLVVETAIQEDVDAVGISILSGAHNYIFPELMRLFKEKGVDDVMVFGGGIVPKKDI
                     ENLKEKGVKTIFEPGTSTDEIVRWVGENVTVRDF"
     gene            complement(1639..3306)
                     /locus_tag="VFJ67_04620"
     CDS             complement(1639..3306)
                     /locus_tag="VFJ67_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7289703.1"
                     /translation="MSLEKRTSKELDRARKYWEEEYRGAGKREADYTTISGMEVPPLA
                     TPLDLNGFDYEDKLGFPGSYPFTRGIHSTMYRGRLWTMRQFAGFGSPEDTNRRFKYLM
                     DQGQTGLSTAFDMPTLMGYDSDHERSKGEVGREGVSVSSLQDMEVLFDGIDLGKVTTS
                     MTINCSASIIFAMYLVMAGKKGVSWGKLRGTLQNDMLKEFIAQREWISPPEPSVKIVI
                     DIIEFCAKEVPNWHPVSISGYHIREAGSTAVQELAFTLADGIGYVEESLKRGLDVDEF
                     APRLSFFFNVHNDFLEEVAKFRAARRMWARIMKERFGAKDQKSLILKTHAQTAGASLT
                     AQQPLNNIARVTIQALAAVLGGTQSLHTNSMDETLALPTEEAVTVALRTQQIIAEESG
                     VVNSIDPFGGSYVIESMTDTMEKEAYAYIEKIDELGGIIKAIETGYPQKEIADAAYKY
                     QRELDAGIKTIVGVNKYTSEQGVTIPTLKIEDTVETGQSEKLRELKRKRNNKVVQSKL
                     RGVVTAARRGDNLMPYIIEAVSEYATLGEICDIFREVYGVYRDPGYY"
     gene            complement(3607..>4596)
                     /locus_tag="VFJ67_04625"
     CDS             complement(3607..>4596)
                     /locus_tag="VFJ67_04625"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HET7289704.1"
                     /translation="IKMGLQANEYSNPWLSEGEVPPLPLDRDRIHIISQGVADPLYDY
                     DVIPLTYTESRWMAYVIRADQKDMKAILPEPLNLEDDVIEFWYVIHRNTMIGPYMEMG
                     VTFSASLKAQDGQTYKAGYYPYMYLSNDSGVSAGREPFGFPKKAAYIAAFEHGLNNNY
                     FNMLMERRGYILHTANGRYSDKPLSARPAFYGKTDWGRMNYRITTYPDVTNSVHEVTY
                     LPSDLPPGFGTGHRFMMDASSIRTATGRDIRSWFMQGTPFDYMGGQIPTKEVVGIISF
                     TFNLIIPGAKTIWTKVVKRDPEKDFGSLLYSTPFKYTMRHRFMVPMYSQGGQI"
BASE COUNT         1032 a         1283 c         1046 g         1235 t
ORIGIN      
        1 ggtccctcag cagcgtgaca cgggctttaa tgatataggc gatattcccg aggggcttca
       61 aagcctctat gaaattgatc ccgtgatagc tcgggtcggg ctccgagata agcgggaatt
      121 ttccgctgtc ccattcgaat tttcccgaat cgacgatgac tccgccgatg ctcgtcccgt
      181 ggcctcctat gaatttcgtc gccgaataga cagtaatatc cacgccgaaa tcaaacggtc
      241 tcaataaata tggggacacg gtataatcca gcacaaaggg tattccgttt ttatgcgcca
      301 cgcccgatat cccttcaagg tcggcgatgt cgagcttggg attccctatg gactcgccgt
      361 agactgcctt cgtttttgac gttattgcct tttgcagcgc tttaagatcg ttcgacttaa
      421 cgaactttac aatcacaccg aggcgcggaa aagtgtaatg gaaaaggtta taagtacctc
      481 cgtagaggtt atccatggat acgatctcat cccccgcctg ggcgatgttg aggagcgcta
      541 tagtcgtcgc ggactgtccg ctcgaaaccg cgagcgcacc taccccgccg tccatggctg
      601 cgatccgctt ctcgaaggcg tcctgcgtcg ggttcattat cctcgtgtag atgttcccga
      661 actcgcgcag gccgaaaaga ttggcagcat gttcggtgct tttgaactga aatgatgttg
      721 tctgatagat agggagcgcc ctcgccccgg tcgtggggtc gggttcccat ccgccatgca
      781 gagcgagcga ttccaccctg agttgattat cgattttact catcaccttg ctcctttgtg
      841 ctggttttca tttgttatct taatcgggtt tgttaaaaat gtcgagatag ttttcatagc
      901 cgctgtcccg gaatattgac aataatttaa aaaggataag aggttccagg cgcacggcac
      961 gctccttttt tcccgagaag gcaacctctg tgccattata ttgatattta tatgattccg
     1021 gcgggttatg ttcggaggag tcggataatt atgcctaccg gcatatgtat acgggatatt
     1081 ttaacaccat ctgtatatat aatacgcagg gaagattggg aagcccgaat tgacaatcta
     1141 tgtagaagcc cgctctaatt cccgcttcaa ggaataaatc cgttttctac tcaaaaatcc
     1201 ctgacagtca cgttttcccc cacccatctg actatctcat ccgtcgaagt gcccggttcg
     1261 aatattgtct taaccccttt ctccttgagg ttctcgatat cttttttggg cactataccg
     1321 cccccgaata ccattacatc atccacccct ttctccttaa agagcctcat caactccggg
     1381 aatatatagt tgtgcgcccc ggaaagaata cttattccga cggcatcgac gtcttcctga
     1441 atagccgttt ccacgactaa ttgtggggtg ttatggagcc ccgtgtaaat cacttcgtaa
     1501 cccgcgtccc tgagcgctct cgaaattact ttaacgcccc tgtcgtgtcc gtcgagcccg
     1561 accttgccta taagtatcct taccgctctc ttgctcgtca ttataattcc tccggtttgg
     1621 tatccggttc aggcgtaatt aatagtaacc cgggtcgcga tagacaccgt acacttccct
     1681 gaatatatcg catatctccc ccagggtcgc atactcgctc acggcttcga taatatacgg
     1741 catgagattg tcgccgcgcc gggccgcagt aacaacgccg cggagctttg attgcacgac
     1801 cttgttgttc ctcttccttt tcaactcacg gagcttctcg ctctgaccgg tctccacagt
     1861 gtcttcgatc ttcagggtcg ggatggttac accctgttcc gaagtgtatt tattgacccc
     1921 gactatcgtc tttatccccg cgtcgagctc cctctggtac ttatacgccg catccgctat
     1981 ctccttctgc ggatacccgg tctctatggc ttttataatt ccgccgagct cgtctatttt
     2041 ctctatatac gcgtaagcct ctttctccat cgtgtccgtc atagactcga ttacgtaact
     2101 gcctccgaag ggatcgatag aattaaccac accgctctct tcggcgatta tctgctgggt
     2161 cctgagggcc acggtcacgg cttcctcggt cggtaaagcg agtgtttcgt ccatcgaatt
     2221 cgtatggagg gactgcgtcc ctcccaacac agccgcaagc gcctggatcg tcactcttgc
     2281 tatgttattc aagggctgct gtgccgtaag actcgcccct gcggtctgcg catgcgtctt
     2341 taaaatcaga gacttctggt ctttcgcccc gaacctctcc ttcatgatcc tggcccacat
     2401 cctcctcgcg gcccggaact tcgcgacttc ctcgagaaaa tcgttatgga cattgaagaa
     2461 gaaagacagg cgcggcgcga actcatcgac gtcgaggccg cgctttaggc tctcctccac
     2521 atatcctatt ccatccgcga gcgtaaacgc gagctcctgg acggctgtgg accccgcctc
     2581 cctgatatgg taacccgaga tcgaaaccgg atgccagttc ggaacttcct ttgcgcagaa
     2641 ctctattatg tctatcacta tcttcacgga tggctcaggg ggggatatcc attcccgttg
     2701 agcgataaat tccttgagca tgtcattctg cagcgtcccc ctgagctttc cccagctaac
     2761 ccctttcttc ccagccatga ccagatacat agcgaaaatt attgaagcac tgcagttaat
     2821 ggtcatcgag gtcgtcactt tcccgaggtc tatcccgtcg aacaggacct ccatgtcctg
     2881 aagggaggaa acggaaaccc cttcccttcc cacttccccc tttgagcgtt cgtggtcgga
     2941 gtcataaccc atgagtgtgg gcatatcgaa tgcggtgctt agaccggtct gtccctgatc
     3001 catcagatac ttgaaacgcc tgttggtatc ctcggggctt ccgaagcccg cgaattgacg
     3061 catggtccag agccgtcccc ggtacatcgt actgtgaatg cctcttgtaa acggatagga
     3121 gcccgggaac ccgagcttgt cctcatagtc aaagccgttc agatcgagcg gtgtcgcgag
     3181 tggagggacc tccatcccag agatcgtggt ataatcagct tccctcttcc cggcgccccg
     3241 gtactcttct tcccagtact tcctggccct gtcgagttct tttgacgtcc tcttctctaa
     3301 actcattaat tcttctcctt gtaaaaatat aaaagtcgag cttatccgcc ggtcgcatat
     3361 ttgcgattta aagccatacc ttgtccctct gcttaatgca gttttgattc aagtctcaga
     3421 tttatacgag aacccatcta aatatgtttc cgaccgtata aaattcacaa ccccgcactg
     3481 cgtattatgt tcctatcctg taacgcccca ggagacgtca caggatagga ataggaaata
     3541 atcacgcaat gggttcagac aaatcatgaa tacaaaccgc cttccggtcc tgtttcctca
     3601 ctcctgtcag atttgcccgc cctgactgta catcggaacc atgaagcggt ggcgcatggt
     3661 gtacttgaac ggagtggaat aaagaagcga tccgaaatct ttctccggat ccctcttcac
     3721 aaccttagtc cagatggtct tagcacccgg aataatcaga ttgaacgtga agctgattat
     3781 tcccacgact tctttagtcg gaatctggcc gcccatataa tcgaatggtg tgccctgcat
     3841 aaaccaggaa cggatatccc tgcctgtagc cgtgcgaatg ctagacgcat ccatcatgaa
     3901 tcggtgcccg gtcccaaaac ccggcggtag atcagatgga agataggtaa cctcatgtac
     3961 actgtttgta acgtcggggt aggtagtgat acggtaattc atgcggcccc aatcggtctt
     4021 cccgtaaaaa gccggtcttg ctgaaagagg cttgtcggaa taacgcccat tagccgtatg
     4081 gagaatataa ccgcgccgct ccatgagcat attaaagtaa ttattattga ggccatgttc
     4141 gaatgccgct atataagcag ccttcttcgg aaacccgaac ggctcccttc ccgcggaaac
     4201 ccccgaatcg tttgaaaggt acatatatgg ataatacccc gctttataag tctggccgtc
     4261 ttgggctttt aatgaagcag agaaagtcac tcccatctcc atatacggtc cgatcatagt
     4321 attcctatgt atgacatacc agaactcgat tacatcgtcc tccaggttta ggggctcggg
     4381 gaggattgcc ttcatgtcct tttgatcggc tcgtatgacg tatgccatcc accggctctc
     4441 cgtatatgtg agcggaataa catcgtagtc atacaagggg tctgcaacac cctgacttat
     4501 aatgtggatc cggtccctgt cgagcggtaa tggcggcacc tcgccttcac tcagccaagg
     4561 attgctatat tcattggctt gtaatcccat cttaat
//
LOCUS       DASSJH010000215         4500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_704177, whole genome shotgun sequence.
ACCESSION   DASSJH010000215 DASSJH010000000
VERSION     DASSJH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704177"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            521..3409
                     /locus_tag="VFJ67_04630"
     CDS             521..3409
                     /locus_tag="VFJ67_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HET7289705.1"
                     /translation="MLDRRAEADNLGITDAVMQYVNRYYVDKSIIDPKNMLVLGLDRM
                     EQVVDKVLVEFPDGEDSTSFQVQAGSEKATFDLSGVQSLNDVTDRLEQVFTFVAPHLT
                     PNDPKINDIEYAVLDQMLNSLDPHSGIITPQVYKEFMIETEGSFGGLGIVIGVRDGDL
                     TVISPIEGTPAYRAGIEPNDKIVQIGNESTINMSLIEAVSKLRGPKGTEVAIYIMREG
                     FPAPREYKLVRDTIVIESVETFELNDGIEYVRIRDFQKNTLDSIINNIKNDGAEDPKG
                     IILDLRGNPGGLLDQAERISDLFLKSGVIVTTKIGNSAKRYHANDKDQQYNGSIVVLV
                     DAGSASASEIVAGALKNNERAIVIGERTFGKGSVQQIFDLNDGSALKLTIAQYLTPGD
                     KSIQDVGVTPDIILNPTVITDDTIVFHPNSDNTIPEKPWQKKKKTVNKVLEKPVYSIT
                     YLDNAKAVKTGGEEEEETPEEALSREEKRKKLDEDFYVSLAREIISSSSAPSRKESLG
                     EIQGDITKISKSEEGKIVDKWKLMGVDWSNEKSAGAKPSVDVRITPEIPAGNAGQKVG
                     IDVEVENTGKEPLYRLLATTKSENSVFDGKELIFGKVNPGEKRSWKTSFEIPKWALTR
                     EDWVNLKFEDGNDSHIPDYRFSVNTHGLERPLYAFNYEIVDDGRYGSSGNGNGVPEPG
                     EIIAFAAKVKNAGQGTSEKTLLTLKNNSGDKIFLEKGRAEFENLKPGEVMEAAFTFDV
                     NNTDSPLDLELQIVDDVFKEGLIGKALIPGNTEGAAFQKMDSTVTVAEADTPVRGGSY
                     KEAPIIALSSQGATFTALGQSGDWVKIKLNENSAGWINKGKTVISKSAGPGQPQGVTM
                     LKDTFEAPPVINVTGVPVTTDSQMITINGDVNDGDGVELVSVFIGEDKVALLPSTKTD
                     VPLSVKIKLEDDVNLITIIAKDSKGLLSKQSFVVRKDEEVVRKAEEKG"
     gene            3419..4348
                     /locus_tag="VFJ67_04635"
     CDS             3419..4348
                     /locus_tag="VFJ67_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7289706.1"
                     /translation="MAKFVLSRTLTGAFVIFAVVTITFFLLRTLPGGPFDTEKKLPPQ
                     IKKNIETKYHLDEPVWKQYLTYLRSLALGDFGPSYKYIDRSVNEIIGETLPVSVELGL
                     VSLILAVTLGCPIGIASAAKPGGAVDFASVSVATALVSVPTFVTGALLIYIFSVELRW
                     LPAALWGSPAHAVLPALTLAAGPAAYIARLIRASMLDTSRALFVRTARAKGLSGFRVV
                     TKHILRNALIPVVTVLGPITAFLVTGSFVVEHIFAIPGTGRFFVMAVSNRDYPLVMGI
                     TIVYTIILVLANLIVDVLYVVLDPRIKFDKGGI"
     gene            4351..>4500
                     /locus_tag="VFJ67_04640"
     CDS             4351..>4500
                     /locus_tag="VFJ67_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7289707.1"
                     /translation="MRNIKITIEYDGSGYVGWQRQPSGPTIQETIETSLMTITGERVK
                     LLGSGR"
BASE COUNT         1190 a         1191 c         1346 g          773 t
ORIGIN      
        1 acacacccag gcccggggaa aatccaaggg agcgaggccc ggcatcataa accggcgtac
       61 cctggcggcc cggagaccgg aacccgcgcc tgacgccccc ctttaccagg cgggtctccc
      121 gcatcccagg cggcgcgggg ccgaaagccc gtgcccggaa gcccgtgata tatgatatag
      181 catatatata tatcatatta cagatgcgaa gctccgcgca cacaaaaaac caacatccgc
      241 cttaaaatct gttaaaatta ttcatcttcg gccaaacgtc ccggtgcggt taatccggat
      301 accggacacg aaccggaact gagcaaaaac ggctgcttcc cgacaatttg aatttgattg
      361 ccgttcagcg cggtgtgata atatctaaca tgcagaccag acaaaaattt gaggagaacg
      421 aattttgaat aaatttacgg atagagccaa aaaagcattg gtgttaacag ccgttttcgc
      481 tgccctcgcg tacgccctgc ttttatcccc ggggtcaggc atgctcgacc ggagagccga
      541 ggctgacaac ctcggtataa ccgacgccgt aatgcagtac gtgaaccgct actacgtcga
      601 caagtcgatc atagacccga aaaatatgct cgtactgggc ctcgacagaa tggagcaggt
      661 cgtggacaag gtgctcgtcg aattcccgga cggggaggac agcacgagct tccaggtcca
      721 ggcgggcagt gaaaaggcga ccttcgatct aagcggcgtc cagagcctga acgacgttac
      781 agacaggctg gaacaggtgt tcacgttcgt agcgccgcac ctaacgccga acgaccccaa
      841 gataaacgat atagagtacg cagtgctgga ccagatgctg aattccctcg acccgcattc
      901 agggataatc acgccgcaag tgtacaagga gttcatgata gaaacggagg gtagcttcgg
      961 agggctcggc atcgttatcg gcgtgaggga cggagacctc acggtcatct ccccgatcga
     1021 aggcactccg gcctacaggg cgggcataga gcccaacgac aagatcgttc agatagggaa
     1081 cgaatcgacg atcaatatgt cgctcatcga ggccgtcagc aagctcaggg ggcccaaggg
     1141 gaccgaggta gcgatataca taatgaggga aggcttcccg gcgcccaggg aatacaagct
     1201 ggtcagggac acgatcgtga tagagagcgt cgagactttc gagctcaatg acggaatcga
     1261 gtacgtgagg atcagggact tccagaagaa tacgctcgac agcataataa acaacataaa
     1321 gaatgacggc gcagaggacc cgaagggcat tatcctcgac ctgaggggca acccgggcgg
     1381 gctgctggac caggcggaaa ggatatccga ccttttcctg aaaagcggcg ttatagtcac
     1441 gaccaagatc ggcaattccg caaagcgtta tcacgcgaac gacaaggacc agcaatacaa
     1501 cggcagcatc gtggtgctgg tcgacgcggg cagcgcgagc gcttccgaga tcgtggccgg
     1561 ggcgctcaag aacaacgaaa gggctatagt gataggggaa aggaccttcg ggaaaggatc
     1621 cgtacagcag atattcgatc taaacgacgg ctcggctctc aagcttacga tagctcaata
     1681 cctgaccccg ggagacaagt ccatacagga cgtcggagtg acgccggata tcatactgaa
     1741 cccgaccgtc ataacggacg acacgatcgt tttccatccg aactccgaca acacgatccc
     1801 ggagaagccg tggcagaaaa agaagaagac cgtgaacaag gtcctggaga aacccgttta
     1861 ctcgatcaca tacctcgata acgcaaaggc cgtcaaaacc ggcggggaag aagaggaaga
     1921 gaccccggaa gaggccctga gccgcgagga aaagaggaag aagctcgatg aggactttta
     1981 cgtttcgctg gccagagaaa taatcagctc ctcctcggcc ccgtcaagga aagaatcgct
     2041 cggggagata cagggggaca tcaccaagat cagcaagagc gaggaaggga agatcgtcga
     2101 taaatggaag ctcatgggcg tggactggtc gaacgaaaag tcggcgggcg ccaaaccttc
     2161 cgtcgatgtc aggattactc ccgagatccc cgccgggaac gcaggccaga aggtcgggat
     2221 agacgtggag gtggagaata cggggaagga gcctctctac aggctgctcg cgaccacaaa
     2281 atcggaaaat tcggttttcg acgggaagga gcttatattc ggaaaggtga atccgggcga
     2341 gaagaggtca tggaaaacgt cgttcgagat accgaagtgg gccctcacga gggaagactg
     2401 ggttaatctg aagttcgagg acggaaacga ctcgcatata ccggactata ggttttcggt
     2461 caatacacac ggactcgaga gaccgctcta cgcgttcaat tacgagatag tggacgacgg
     2521 caggtacggg tcgagcggca acggcaacgg ggtccccgag ccgggagaga tcatcgcttt
     2581 tgccgcgaaa gtgaaaaatg cggggcaggg gacatccgag aagaccctcc tcacgcttaa
     2641 aaacaactcg ggtgacaaga tattccttga aaaagggagg gcggaattcg agaacctcaa
     2701 gcccggagag gtaatggaag cggcatttac gttcgacgtt aacaacacgg actcgccgct
     2761 cgacctcgag ctccagatcg tggacgacgt attcaaggaa ggtctcatag ggaaagcact
     2821 cattccgggg aatacggaag gcgctgcctt tcagaagatg gattccacgg tgactgtggc
     2881 cgaagccgac acgcccgtga gaggcgggag ctacaaggaa gcgccgataa tagcactctc
     2941 gagccagggg gccacgttca ccgcgctcgg gcagagcggc gactgggtca agataaagct
     3001 gaacgagaac tcggccgggt ggatcaacaa gggcaagact gtaatttcga agtcggcggg
     3061 accggggcag ccgcagggag tgacgatgct gaaggatact tttgaggctc cgcccgtcat
     3121 aaacgtgaca ggcgtgccgg tcacgaccga ttcacagatg atcacaatta acggagacgt
     3181 gaacgacggg gacggcgtag agctggtatc ggtattcata ggcgaagaca aggtagcgct
     3241 gctgccgtcg acgaaaacgg acgtgccgtt atcggttaag atcaagctcg aagacgatgt
     3301 aaacctcatc acgatcatcg ccaaggattc gaagggcctc ctgtccaagc aatccttcgt
     3361 ggtgagaaaa gatgaagagg tagtgagaaa agcggaagag aaagggtaga gcggcttgat
     3421 cgcgaaattc gtcctcagcc gcacactgac cggcgcattc gtaatattcg cggtcgtgac
     3481 cataacgttc ttcctgctga ggactctgcc cggggggccg ttcgacacgg agaagaagct
     3541 acccccgcag atcaagaaga atatcgaaac caaataccac ctggacgagc ccgtctggaa
     3601 acagtacctg acgtacctgc gaagcctcgc gctcggggat ttcgggccgt cctataaata
     3661 catagacagg tccgtgaacg aaataatcgg cgagacgctc cccgtttcgg tcgagctcgg
     3721 gctcgtgagc ctcatcctcg cagtgacact cggatgccca atcggcatcg cctcggccgc
     3781 gaaacccggg ggggccgtgg atttcgcctc cgtgtccgta gcgaccgcgc tcgtatccgt
     3841 tccgaccttc gtaacgggcg ccctacttat ttatatattt tcggtcgagc tcaggtggct
     3901 cccggcggcg ctctggggga gccccgccca cgcggttctt cccgcgctca cactggccgc
     3961 gggccctgcc gcatatatcg cgcgcctgat aagggcaagc atgctcgaca cgtcaagggc
     4021 cctcttcgta agaacagcgc gggccaaggg gctgagcggc ttcagggtgg tcacgaagca
     4081 catactgagg aacgcgctca tccccgtcgt cacggtgctg ggaccgataa cggcgttcct
     4141 cgtaaccggg tcgttcgtcg tcgagcacat cttcgccata ccgggcacgg gcaggttctt
     4201 cgtcatggcc gtctccaaca gggactaccc gctcgtgatg gggataacga tcgtgtacac
     4261 gataatcctc gtgctcgcga acctgatagt ggacgtgcta tacgtcgtac tcgacccgag
     4321 gataaaattc gacaagggcg gcatataagg ttgaggaaca tcaagattac gatcgagtac
     4381 gacggctccg gatacgtggg ctggcagaga cagccatcgg ggccgacgat acaggaaacg
     4441 atagagacgt cgctcatgac gataaccggg gagagagtga agctactggg ctcgggaagg
//
LOCUS       DASSJH010000216         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_487637, whole genome shotgun sequence.
ACCESSION   DASSJH010000216 DASSJH010000000
VERSION     DASSJH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487637"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VFJ67_04645"
     CDS             complement(<1..1307)
                     /locus_tag="VFJ67_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7289708.1"
                     /translation="MDSDDRTTTDPQPGELMKKTKRELVEQIESLYRNNSELRKSLEE
                     MDAGLESVKHLGSILEESIFEIFVLDRTTLAFIHANKKARENIGYPMRDLCGITVFEL
                     SKYFGEDRLVKMAEPLREGKSDKVEYTATHTTHDGTAHSVEVHLQSSTFDNTPVVVAV
                     MVDITKRAQAEERLKETLTQLSKINKYETIIGIVTRTVHNSVNLKTVMENAVQAIYQN
                     MDAAKNVAIFLVEGAEAVLHAHRGLPDWFAEKTKRVQFPIGATWRTIVQRKTAYVPDT
                     DAGQQMDPAGVALGIKSYVSIPLKNDGKVLGVINISTDVKNSFRADELRLLDIVSRQI
                     EIAINNARFTESLIASEKALEEKVTILSRKESYEKIIYSIASRVHSSVDFDEVLRLTI
                     NGLRQNIKNADFIAVYFVEGNEAVMKSVYGHDLNFRKKVEKIP"
     gene            complement(1603..2091)
                     /locus_tag="VFJ67_04650"
     CDS             complement(1603..2091)
                     /locus_tag="VFJ67_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET7289709.1"
                     /translation="MKRKPESKKGNGHPKFENLKLAECLDCVCYEFRKTARFVINYYD
                     TALKDADLKSNQFIILVSVGYLESPNFKKLAEFVGIDQSTLARNLTTVEKQKLVSVRA
                     GKNRREKLISLTKKGERKLVSSFPLWKKAQGRLVDGLGTDNWKNIQKELTDVVSITKS
                     LN"
     gene            complement(2173..>2681)
                     /locus_tag="VFJ67_04655"
     CDS             complement(2173..>2681)
                     /locus_tag="VFJ67_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7289710.1"
                     /translation="IINGQPGTVEQINFRTTVIRDLQGIVHVFPNGTITQLSNQSKGW
                     SRYVIDMGVAYKENVDRVMDVMMGIGEGLRKDEKFGPLILEPLEILGVDNFGDSSVVI
                     KCMIKTLPLKQWDVGRELNRRIKNTFDREGIEIPFPCRSLYYGSGSPPFGVALGGDAE
                     AGAAETGS"
BASE COUNT          504 a          754 c          675 g          748 t
ORIGIN      
        1 tagggtatct tttccacttt ctttctgaaa ttgaggtcgt gaccgtaaac cgatttcatg
       61 accgcctcgt tcccctcgac gaaatatacg gcgatgaagt ccgcgttctt tatgttctgc
      121 ctgagtccgt ttatcgtgag acgaagcact tcgtcgaaat cgaccgagct gtggactctc
      181 gaggctatcg aatagattat cttctcgtag ctctcctttc tggagaggat cgtcaccttt
      241 tcctcgagcg ccttttccga ggcgatcagg gattccgtaa acctcgcgtt gtttatcgcg
      301 atctctatct gtctcgatac tatgtcgagg agcctgagct cgtccgccct gaatgagttc
      361 ttaacgtccg tggatatgtt tatgacgccg agtaccttac cgtcgttctt gagcggtatc
      421 gagacgtagc tcttaattcc gagcgcgacg cccgccgggt ccatctgctg cccggcatcc
      481 gtgtccggca cgtacgcggt cttcctctgc actatcgtcc tccacgtggc ccctataggg
      541 aactgcaccc tcttcgtctt ttcggcgaac cagtcgggca ggcccctgtg ggcgtggagc
      601 accgcctccg ccccctcgac gagaaagatg gccacgttct tcgcggcgtc catgttctgg
      661 tatatcgcct gcaccgcgtt ttccataacc gtcttgaggt tcacggagtt gtggaccgtt
      721 ctcgtgacta taccgattat ggtctcgtac ttgttgatct tggaaagctg tgtgagcgtc
      781 tccttgagcc tctcctcggc ctgtgccctc ttcgttatat ccaccatcac ggccacgact
      841 acgggcgtat tgtcgaatgt agagctctgg aggtgcacct ccactgaatg agccgtcccg
      901 tcgtgggtcg tgtgggtcgc cgtgtattcg accttgtccg atttaccctc ccggaggggc
      961 tccgccattt tcacgagcct gtcttccccg aagtacttcg agagctcgaa taccgtgatt
     1021 ccgcacaggt ctctcatcgg gtagcctatg ttctccctcg cttttttatt tgcgtgtatg
     1081 aaggcgaggg tcgtcctgtc gaggacgaat atctcgaata tcgactcctc gagtatcgag
     1141 cccaggtgct tgacgctttc gagaccggcg tccatttcct cgagtgactt acggagctcg
     1201 gagttattcc tgtagaggga ctctatctgt tcgacgagct ctctcttggt ctttttcatg
     1261 agctcgcccg gctgcgggtc tgttgtcgtt cggtcgtcgc tatccattcc gggctccgga
     1321 gaaaaaatat gtcggattca aagggtttaa ttgcaagtat caagccgttt ctgaaatctg
     1381 tcaacgacgg ggagatgtat ttctttcgtg cgggacagcc cgagacggta taggcaggtt
     1441 ttgataacgc ctgaaggatg cgtgtttgaa attaccggcg atcaatatcg ataagttgaa
     1501 cgatttttaa atggcgctgg aaaggttcag gaaggcgcaa catccgtaat atatgctcgg
     1561 gtgcatacga cgatgtaaac aagtcggggc gaccggccgc tctcagttga ggctctttgt
     1621 tatgctgact acgtctgtaa gctctttctg gatgtttttc cagttatccg ttcccagccc
     1681 gtccacaaga cgtccctggg cctttttcca aagcggaaaa gagcttacga gcttacgctc
     1741 gcctttcttg gtaagggaaa tgagcttctc cctccggttc ttaccggccc ttacgctcac
     1801 gagcttctgc ttctcgaccg tggtcagatt gcgggcgaga gtgctctggt ctattcctac
     1861 gaactcggcg agtttcttga aattgggcga ctccaggtat ccgaccgaca caagaataat
     1921 gaactggtta ctcttgagat cggcgtcctt gagagccgtg tcataataat ttatgacgaa
     1981 acgcgcggtc ttacggaatt cataacaaac gcaatccagg cactcggcaa gcttgagatt
     2041 ctcgaacttc gggtgcccgt tccccttctt tgactcaggt tttctcttca tttcagtcta
     2101 tatattaccc atgtataaca ccgcagtaaa gttatagttt agggttacaa acgggcacag
     2161 ttgcgcgcag cgtcacgacc ccgtttccgc cgcgcccgct tccgcgtctc cgccgagcgc
     2221 gacgccgaag ggcgggctcc cgcttccgta ataaaggctc ctgcatggga atggtatctc
     2281 tattccctcc cggtcgaacg tgttctttat cctcctgttt agctccctgc cgacgtccca
     2341 ttgcttgagc ggcagcgtct ttatcatgca ctttatgacg acgctcgagt ctccgaaatt
     2401 atcgaccccc aggatctcga ggggttcgag tatgagcggg ccgaatttct catccttcct
     2461 tagcccttcg cctatcccca tcatcacatc catgacgcgg tcgacgttct ccttgtaggc
     2521 gacgcccatg tctatcacgt atctcgacca gcccttgctc tggttagaga gctgagtaat
     2581 cgttccgttc gggaaaacgt ggacgatgcc ctggaggtcc ctgattacag tcgtcctgaa
     2641 atttatctgt tccactgtcc ccggctgccc gtttattatc g
//
LOCUS       DASSJH010000217         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1024737, whole genome shotgun sequence.
ACCESSION   DASSJH010000217 DASSJH010000000
VERSION     DASSJH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024737"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFJ67_04660"
     CDS             complement(<1..313)
                     /locus_tag="VFJ67_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289711.1"
                     /translation="MRRENFSADTGLEYNLKFFTQFIINPARTGAVLPSSNGLCEYMT
                     DLAGLNDVSTVIELGSGTGVITEKIMRKKAESTTFFALEINPAFVEATKNKCPDAIVY
                     QS"
     gene            565..1320
                     /locus_tag="VFJ67_04665"
     CDS             565..1320
                     /locus_tag="VFJ67_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7289712.1"
                     /translation="MQDEIIIDVRDVYKAFGPKEVHMGLTLAIKKGENITVLGGSGSG
                     KSVLLKEITGLLKPDSGDVIVEGENIIPMDETDLVNVRKKMGMLFQGSALFDSLTVEE
                     NIAYPLRENTKLPESEIKDVVAQNLELVGLPGIEDKMPSDLSGGMKKRVGLARAMAMN
                     PQILLYDEPTTGLDPPNISRINQLIRHMQAQFGITGVIITHDVKSAFEISDRIAFLCH
                     GKIIFTGTVKEAEETEIEILSDFIHGRMGGADE"
     gene            complement(1352..1825)
                     /locus_tag="VFJ67_04670"
     CDS             complement(1352..1825)
                     /locus_tag="VFJ67_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289713.1"
                     /translation="MSKYLLLLIAAALVLLYLFFPRGDEPREIEAVFDEMKEAASNKD
                     TDGVAEHFSMQYKDEYGATYHVVKKVIGNVFRKYDSIRVSYSGLSVVFSENESGEKEA
                     TANLDVTVTGYSSGMSYDLIGSEDSPDNVTVTLEKSGLGEWKITGVEGLDAQESY"
     gene            complement(1835..>2071)
                     /locus_tag="VFJ67_04675"
     CDS             complement(1835..>2071)
                     /locus_tag="VFJ67_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289714.1"
                     /translation="RSFGGDDAFLPFLRAVSPRVLITGEPVPSRDGDDPGLRKALADI
                     TVFDTSADGGVTLKTDGSGMSVGNYAGEKKANIK"
BASE COUNT          493 a          508 c          567 g          503 t
ORIGIN      
        1 aggattgata aacaatggcg tcggggcact tatttttggt cgcctcgacg aaggcggggt
       61 ttatctcaag cgcgaaaaag gtagtgcttt cggctttctt gcgcattatc ttttcggtaa
      121 tcacacccgt gcccgagccg agctctatga ctgtggaaac gtcgttaagt ccggcaaggt
      181 ccgtcatata ttcgcagagc ccgttgctcg atgggagcac ggctcctgtc ctcgcggggt
      241 ttatgatgaa ctgcgtgaag aatttaaggt tgtattcgag ccctgtgtcg gcgctaaagt
      301 tttctcttct cattttgaat tgtcccacac cgctgcagag ccgtaatagg tgtggatatt
      361 agggtcgtgc aaccggtctt ttaagcccat gtttatacat cggttgattg aaaaagtcaa
      421 ttgttaaagt gaattgatat tatttgggtg cgctgcctga ctattttgac aaataattgt
      481 tgtataatta gaatcccaca tgactcactg agaatatatt taccgttttt aatttatcaa
      541 cactgttaaa acggcggaaa tcggatgcag gacgaaataa ttatcgatgt tcgggatgtt
      601 tataaagcgt tcggccccaa ggaggtccac atggggctca ccctcgctat caagaaaggg
      661 gagaacatca ccgtgctcgg gggaagcggt tccggtaaga gcgtgctgct gaaagaaatc
      721 acgggacttc taaagcccga ttcgggcgac gtcatcgtgg agggtgaaaa tatcataccc
      781 atggatgaga ccgatctcgt caacgtaagg aaaaaaatgg ggatgctttt ccaggggtcg
      841 gcgctcttcg attctcttac cgtagaggag aacatagctt atccgctaag ggaaaataca
      901 aagctgcccg agagtgagat aaaggacgtc gtggcccaga acctcgagct cgtggggctt
      961 ccggggatcg aggacaagat gccgtccgat ctcagcggcg gtatgaagaa aagggtcggt
     1021 ctcgcgaggg cgatggcgat gaacccgcaa atcctcctct acgacgagcc cacgacgggg
     1081 ctcgatccgc ccaacataag caggatcaac cagctcataa ggcacatgca ggcccagttc
     1141 ggcataacgg gcgtcatcat cacgcacgac gtaaagagtg cgttcgagat atcggacagg
     1201 atagcgtttc tctgtcacgg gaagattata tttaccggga ccgtgaaaga ggcggaggaa
     1261 accgaaatag agatcctgag cgatttcatt cacggaagga tgggcggggc cgacgagtag
     1321 ctcattaccc cgttattgaa tttatctgcc cttaatagct ttcctgtgcg tcgagtcctt
     1381 cgacacctgt gatcttccac tcgccgaggc ctgacttttc taacgtgacc gtgacgttat
     1441 ccggggaatc ttcgcttcct atcagatcgt aagacattcc ggatgagtat ccggtgaccg
     1501 tgacgtcgag gttggcggtg gcctctttct cgccggattc gttttcgctg aagactacgg
     1561 agagaccgga ataagaaacc cgtatgctgt catactttcg gaaaacgttt ccgatgacct
     1621 tctttaccac atgataggta gcgccgtatt cgtctttgta ttgcatggaa aaatgttcgg
     1681 cgacgccgtc cgtgtcttta tttgacgcag cctctttcat ctcgtcgaat accgcctcta
     1741 tctcccgcgg ctcgtcgccg cgcgggaaga aaagatacaa gagaacgaga gctgccgcta
     1801 taagaagcag tagatatttg gacaccggga aagtttactt tatgttcgct ttcttttcac
     1861 cggcgtagtt cccgacgctc atgccggagc cgtccgtttt gagcgtaacc cctccgtccg
     1921 ctgaagtgtc gaacacggta atatccgcga gggccttgcg caggccggga tcatcgccgt
     1981 ctcgagacgg taccggctcg cccgttatca ggacccgcgg ggagaccgcg cggaggaacg
     2041 gcaggaacgc gtcgtccccc ccgaacgagc g
//
LOCUS       DASSJH010000218         9228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1024834, whole genome shotgun sequence.
ACCESSION   DASSJH010000218 DASSJH010000000
VERSION     DASSJH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9228
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024834"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            584..1156
                     /locus_tag="VFJ67_04680"
     CDS             584..1156
                     /locus_tag="VFJ67_04680"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7289715.1"
                     /translation="MEQGTVRNADQELIERLRKGDKNAFREIYARYSQIVFNLAFRML
                     RSREEAEEVVQEIFLQVWNKADSYDSGRGAISTWIVNIARSRSIDKLRMLGYREQTTE
                     LIEERVNSKSDLSRIIEDREESRKVIREALDSLPDNQRMAIEMVFFEGLTHIEAAERL
                     NEPVGTIKTRIRLGVAKLREKIAPYVGDLM"
     gene            1157..1930
                     /locus_tag="VFJ67_04685"
     CDS             1157..1930
                     /locus_tag="VFJ67_04685"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET7289716.1"
                     /translation="MAHKPEYEDQIALYSLGLLEGAELREVEEHLAEGCEICAAMLRD
                     SDTVFTNLPLCLEDAPLPEDLEKKILGRLEAREPVREKSSIFDFWKNLSPVWLNLGAA
                     VSLAVIIFLAVTNISLRNEIELQRQNLTELEARLDKEKEMIAFVMNPGVNKVELGSKM
                     PDINASGRIYWDKEKDKGLFLVSSIPLPKEGMTYQLWAIEDGKPVSMGVFDVDRSGNS
                     MMELKPMPEHDGPMQFAVTLEPAGGVPQPTGDMYLYGSL"
     gene            2076..2867
                     /locus_tag="VFJ67_04690"
     CDS             2076..2867
                     /locus_tag="VFJ67_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HET7289717.1"
                     /translation="MILPPCMRRSADFYLRLFFFLLLILTVNAEPSSAFDHAYAVYGS
                     VLSVYVKNGLVDYSGLKSDPGGLRSFLESTERVQRREFESWPRREQLAFLINVYNART
                     LELVIDNYPVKSIKDTGSGGKGPWDRPIVKVFGETITLNELENGTIRKNYKEPRIHFA
                     LVCAAMGCPPLLDKPYAGAGLDAQLETRTKKFLSDTGKNSLDEKSKTLRLSPIFEWYA
                     GDFEAEAGSVAGFLKEYYGGLPLDGYIIVYTDYDWSLNDNSDKME"
     gene            2938..5319
                     /locus_tag="VFJ67_04695"
     CDS             2938..5319
                     /locus_tag="VFJ67_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7289718.1"
                     /translation="MFGLIKKYTRWLHTQWPAGIVEKLPEVKEDYSSNVPGLYIVGDL
                     TGIPLLKFSSDSGARAVMHILEDESFLKSRETRKDGDVLDLVIVGAGVSGMAAALEAK
                     RSGISFEILEATEPFSTIVNFPKGKPIYTYPTEMVPAGDLQFTKDVKEPLVEEIKEET
                     LGRGIKPRIVRVEKIANRGGYMEVVLPEGASLKALRVIVGIGRSGNFRMLGVPGEDKD
                     KVFNRLHDPKDYEGKNVLVVGGGDSALETSIAIARAGGNVTHSYRNEEFSRPKPENVE
                     SLRMLMADPMADVSIETPSSERVTTSTGGFMPGEHKPGKITLMMKSRVRNIGDTDVTL
                     TDRDGKDITIPNDAVFTMIGREAPLDFFRRSGVSIKGEMNAKSITAMVIFLLFMTFVY
                     NWKAGGPLTKLFEKHKWFPFNLPEFFNSFGESFTRMVNDPSTLVGTLSITLSEPGFYY
                     SVLYSIIIIIFGTLRIRRRKTPYITVQTLSLMTFQLLPLFLLPYILLPYLGHNGVFDT
                     GFMKTVGDALFPAANYGHGREYWRAFGFILAWPLFVWNVFSSEPLWTWLVISVVQTFV
                     LIPLIIYFWGKGAYCGWICSCGAMAETLGDTQRHKMPHGAKWNRLNMVGQVILAVAFI
                     LLIARVLSWTFPNTALGQSIYRFYEGAFYGWKPFGIELNYKYVVDLVLAGIVGYGLYF
                     WYSGRVWCRFMCPLAALMHIYSRFSRFRILAEKKKCISCNVCTSVCHQGIDIMNFANK
                     GLPMSDPECVRCSACVQSCPTGVLKFGQINRKSGKVISIDSLAASPVLMREGK"
     gene            5441..6139
                     /locus_tag="VFJ67_04700"
     CDS             5441..6139
                     /locus_tag="VFJ67_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7289719.1"
                     /translation="MTPRIFVIIPALNEEVSIGKVIADIPGGLVSEVIVTDNGSADGT
                     ADIAAAAGATVLREERRGYGYACMKGIEYARSKAEGERPDIVVFLDGDYSDYPEELPI
                     LVKPIIEDGYDMIIGSRTRGERERGALLPQAFFGNALATTLIKWIYGVEFTDLGPFRA
                     IRFDKLLLLGMTDMTYGWTVEMQIKAAKQGLRCGEVPASYRKRIGESKVTGTLGGTVM
                     AGYKILFTIFKHIW"
     gene            complement(6186..7295)
                     /locus_tag="VFJ67_04705"
     CDS             complement(6186..7295)
                     /locus_tag="VFJ67_04705"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET7289720.1"
                     /translation="MSVKDLYSRLKPGNMTYSAKFWVIFIVSLIVLLIFYYILLDRYT
                     PYTSDAYVQAYVLQVAPQVDGQVDHVYIKNNEYVEKGRKLFSLDPRPYEYQVQHLKAK
                     LVQTRQNIDELKSDIAAAGQAVKQSQADHAYASQRYNDLKPLVEKNYIAKLEFDQSVE
                     QLNSTLAKLNQSKADFEKAKQALEYKIDGDYAIIKEVEASLATAEYNLSQTTFYAPTD
                     GYITNLQLTVGSYIKVGDAVLTFVDDNSWWVVANFRENSVGQIRKGQEAEVSLALYPG
                     TIFKAVVESTDWGVSTGQGVPSGFLPRVKDPENWVQLAQRFPVRLRITDIDNDKYKLR
                     IGASVSVAVFTGENFILNALARLWLRIGSLIDFIY"
     gene            complement(7396..7800)
                     /locus_tag="VFJ67_04710"
     CDS             complement(7396..7800)
                     /locus_tag="VFJ67_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289721.1"
                     /translation="MFRIRLALTAFAVLLTISGCAAIQGEQAASTDKMLAAAGFIIVT
                     ADTPEQEQMLNSLTPNKVQFSVRDNKPLYWYADPYNCKCIWTGDQAAYDRYERLVYQS
                     NLVNEEQEAAMMAEQAEFGPGLWGWAGGPWGW"
     gene            complement(7916..>9228)
                     /locus_tag="VFJ67_04715"
     CDS             complement(7916..>9228)
                     /locus_tag="VFJ67_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET7289722.1"
                     /translation="LRSCALTVRPVLNNEMNLFAVFTILITVTAVFSFLNEAFVKLPT
                     TIGVMLASLLASILILTLGYLGFGRTDWAVELVQAADFDHLFMNGMLSFLLFAGASSV
                     NIDELGEYKWTISYLATLGMVIATVLTGFIVWRVYNAIGVDMPLIYALVFGAIISPID
                     AVIVLKLFRRIHPGKTLESVISGESLFNDAVAVVLFVLFVEIQTGETQVTALHIGEFF
                     LKEALGGLALGIGLGYLAYRMIYIIVVRSQDGRVVVALITLSLVSGGYFLAETLDVSA
                     PLTCVVAGLFLARRRRQMPESAEHIRAYMRSLWEVIDDVLNAILFVLIGLEVLVLHLS
                     RPYIICGLLAVPLITAARYISVELPLVFLKLFRRLPSLSGFIMTWSGIRGGVSIALAL
                     SLADSPQQDLIVLMTYIVVVFSITVQGLTLENALKWNSRRTNSE"
BASE COUNT         2247 a         2486 c         2582 g         1913 t
ORIGIN      
        1 cttaccttct ccgattgctc tgtcttggcc ggactcttgt ccggcacctt ggtttcttcg
       61 gttctctcac acccggtacc gagaaacgca atcaacaacg gaactattaa taataatgcg
      121 aacggcctct tcatatacgc ctcctgagca ttcctgaaga atagaaataa tatagccagc
      181 aataacccat acgcaagaaa aataatttat acccctgtcg cataataaag ggcccgagcg
      241 cgagccggag cggttatcaa ccgccgcccg aggggcagcg gagcccggcg cgtccggacg
      301 tcatttaaag tgtccccgag gggttatgaa accggacgga ccgaacgtgc cgtatataca
      361 aaagtaatcg cggataaaac cggatccagc atcatgcggg cggaaaaatg taatgatttg
      421 atgaggctcg ggttttgcgg tccgtttctg agaaccgggc ccggctcaga cagtaactga
      481 atttataggg gaagacccag gtatgagatc cggagacacc cggtggattg acaacgaccc
      541 tgaattaact acaatggttc gaaagtctaa agcttttgcc gccatggaac agggcacagt
      601 ccggaacgca gaccaggaat tgatagaacg gctcaggaag ggcgacaaaa acgccttcag
      661 ggagatctat gcccgttact cgcagatcgt cttcaattta gcattcagga tgcttaggtc
      721 gagggaagag gccgaggagg tcgtgcagga aatattcctc caggtatgga acaaggccga
      781 ctcctacgac tccgggaggg gagcgatctc gacctggata gtgaacatcg cgaggagcag
      841 gtccatcgac aagttgagga tgctcggtta cagggaacag acgacagagc tgatcgagga
      901 gagggtgaac tcgaagtccg atctctctcg tataatagag gacagggaag agagcaggaa
      961 agtgatacgc gaggccctcg attctcttcc ggacaatcag aggatggcga tagaaatggt
     1021 gtttttcgag ggcctgacac atatagaagc ggctgaaagg cttaacgagc ctgtaggcac
     1081 aataaagacc agaataaggc tcggagttgc gaaattgaga gaaaaaattg caccctatgt
     1141 cggggatttg atgtaaatgg cgcacaaacc tgaatacgaa gaccagatag cgctctactc
     1201 gctcggcctc cttgaaggtg ccgagctgag agaggtagag gagcatctcg cggaagggtg
     1261 cgagatatgc gcggctatgc tcagggacag cgatacggta ttcacgaacc tgccgctatg
     1321 cctcgaagac gcgccgctgc ctgaagatct cgagaagaag atactcggca gactggaggc
     1381 cagggaaccg gtccgcgaaa aatcatccat attcgatttc tggaagaacc tgagccccgt
     1441 gtggcttaac ctcggcgcgg cagtctccct cgcggtcata attttcctcg ccgtgacgaa
     1501 catctcacta agaaacgaaa tcgaactaca gcggcagaac ctgaccgagc tcgaggccag
     1561 gctcgacaaa gaaaaagaaa tgatcgcctt cgtcatgaac cccggcgtga acaaggtcga
     1621 gctcggcagc aagatgccag acattaacgc ctcaggaagg atctactggg acaaagagaa
     1681 ggacaagggg ctctttctcg tctccagcat ccccctgccc aaggaaggca tgacgtatca
     1741 gctctgggcg atagaggacg gaaagcccgt cagtatgggc gttttcgacg tggaccgttc
     1801 cgggaacagc atgatggaat taaaaccgat gcctgagcac gatggcccca tgcagttcgc
     1861 tgtcacactc gagcccgcgg gcggcgtgcc acagccgacg ggcgacatgt acctctacgg
     1921 ctcactttaa ataacccgcc gaatcaatta aaatcactaa gtctgcggtt tcgccgggct
     1981 aagggatttc tcggcactgc tcctgaacgt aaatgaaacg aatccgagag tcgctcccga
     2041 gcgtataaac cttttagtat cggagacctc atacgatgat cctgcctcct tgcatgcggc
     2101 ggtctgcaga tttctatctg cggctattct tttttttact cctcatatta acggtcaacg
     2161 cggagccgtc atccgctttc gaccacgcat acgccgtcta cggaagcgtg ctttcggtct
     2221 atgtcaaaaa cggtctcgtc gattacagcg gcctcaaatc ggacccagga ggcctccgct
     2281 cattcctcga atcgaccgag cgtgtccaga ggcgggaatt cgagagctgg cccaggcgag
     2341 aacagctcgc atttctcatc aacgtctaca acgcacggac actcgagctc gtaatcgaca
     2401 attacccggt caagagcata aaggatacag ggagtggcgg gaaagggccc tgggataggc
     2461 cgatagttaa ggtcttcgga gagaccatca cgctcaatga gctcgagaac gggacgatac
     2521 gaaaaaatta caaagagccg aggatacact tcgcactcgt atgcgcggca atggggtgcc
     2581 ctcccctcct cgataaacct tatgcgggag cgggtctcga cgcgcagctc gagacccgga
     2641 cgaagaaatt cctctcggat accgggaaaa actctctcga cgagaagagc aaaacactcc
     2701 gtctctctcc tatcttcgaa tggtacgccg gggacttcga ggctgaagcg gggtcggtcg
     2761 cgggattcct caaagaatat tacgggggac tccctctcga cggatatata attgtatata
     2821 cagactacga ctggtcgctt aacgataatt ccgataagat ggaataacga gccgataacg
     2881 cacataaatg cataacgcgg gtgagcaggc tctaccgacc gcgccggagg aacagacatg
     2941 ttcggactga taaaaaaata tacgaggtgg cttcacactc agtggccggc ggggatagtc
     3001 gagaagctgc ctgaggtgaa agaggattat tcttcgaacg tcccggggct ctacatcgtg
     3061 ggagacctga cagggatacc gcttctcaag ttctcatccg actcgggcgc gagggcggtg
     3121 atgcacatac tcgaagacga gagcttcctc aaaagccgcg agaccaggaa agacggagac
     3181 gtgctcgacc tcgtcatagt gggggctggt gtctcgggaa tggcggccgc gctcgaggct
     3241 aaaaggagcg ggataagctt cgagatactg gaagcgaccg aacccttctc cacgatcgtc
     3301 aacttcccga aggggaagcc catatacacg tatccgaccg agatggtccc ggcgggggac
     3361 ctccaattca ctaaagacgt aaaggagcct ctcgtcgagg agataaagga agagacactc
     3421 ggcaggggca ttaagcccag gatcgtgaga gtcgagaaga tagcgaacag aggcggatac
     3481 atggaggttg tactccccga aggcgccagc ctcaaggcgc tccgcgtcat cgtcggaata
     3541 gggcgctcgg gcaacttcag gatgctgggc gtgcccggcg aagacaagga caaggttttt
     3601 aacaggctcc acgacccgaa ggactacgag ggcaagaacg ttctcgtcgt tggcgggggg
     3661 gacagcgcgc tcgaaacgtc gatcgcgata gcgcgggcgg gcgggaacgt cactcattca
     3721 tacaggaacg aggagttctc tcgccctaag cccgagaacg tagagagcct gaggatgctc
     3781 atggccgacc cgatggcgga cgtatccatc gagacgcctt cctcggagcg tgtcacgacg
     3841 agcacgggag ggttcatgcc gggggagcac aagcccggga agataacgct catgatgaag
     3901 agccgcgtca ggaacatagg ggacacggac gtgaccctca ccgacaggga cgggaaagat
     3961 atcacgatcc ccaacgatgc ggtatttaca atgatcgggc gcgaagcccc tctcgatttc
     4021 ttcagaagaa gcggcgtgag catcaaaggg gaaatgaatg caaagtccat aacggctatg
     4081 gtcatcttcc tcctcttcat gaccttcgtt tacaactgga aagcaggcgg gccgctcacg
     4141 aagctcttcg agaagcacaa gtggttcccg ttcaacctgc ccgaattttt caacagcttc
     4201 ggggaatcgt tcacgcgcat ggtcaacgac ccttcgactt tggtcggaac gctctccata
     4261 accctgagcg agcccgggtt ctattattcg gttctctact cgataataat cataatcttc
     4321 ggcaccctcc gcataaggag gagaaaaacg ccctacataa cggtgcagac gctctcactc
     4381 atgacgttcc agctgctgcc gttatttcta ctgccttaca ttctccttcc ttacctgggg
     4441 cacaacggcg tattcgacac ggggttcatg aagactgtcg gcgacgcact gttccccgcg
     4501 gccaattacg ggcacggccg ggagtactgg agggccttcg gcttcatact ggcgtggccg
     4561 ctcttcgtct ggaacgtctt cagcagcgag cccctctgga cgtggctcgt cataagcgtt
     4621 gtccagacgt tcgtgttgat accgctcatc atctacttct ggggcaaggg agcctactgc
     4681 gggtggatat gctcctgcgg tgcgatggcc gagacgctcg gcgacacgca gcgccacaag
     4741 atgccccacg gagccaaatg gaacaggctc aacatggtcg ggcaggttat acttgctgtc
     4801 gcgttcattc tacttatagc tcgggtgctg agctggacct tcccgaacac cgccctcggg
     4861 cagtcgatat acaggtttta cgaaggggcc ttctacggct ggaagccttt cgggatcgag
     4921 ctcaactata aatacgtggt agatctggtg ctcgcgggga tagtcgggta cgggctctac
     4981 ttctggtaca gcggccgggt gtggtgcagg ttcatgtgtc cgctcgcggc gctcatgcac
     5041 atatactccc ggttcagccg gttcaggata ctcgccgaaa agaagaaatg catatcgtgt
     5101 aacgtatgca cgtccgtatg tcaccagggg atagatataa tgaatttcgc caacaagggc
     5161 ctccctatga gcgaccccga atgtgtcagg tgcagcgcgt gcgtacagtc gtgcccgacg
     5221 ggagtgctga aattcggcca gattaaccgc aagagcggga aggtgattag tatagactcc
     5281 ctcgccgcct cccccgtttt gatgagagag gggaagtaat ccacatcacg caattccgct
     5341 gattcaaaat attcaaattg cggcaaggaa gagaggccgg gctgctttca tatggcttaa
     5401 gagtaagtgt aatatgtagt catctcggca gagaaatttt atgactccca gaatcttcgt
     5461 aataatccct gcgctgaatg aagaagtatc gatcggcaag gtgatcgccg acataccggg
     5521 cggcctcgta tccgaggtca ttgttacaga caacggatcg gcggacggaa ctgccgatat
     5581 tgcggcagcg gcgggcgcga ccgtgctgag agaagaaagg cgcgggtacg gttatgcgtg
     5641 catgaagggt atcgagtacg cacgttcaaa ggccgaaggc gagaggcccg acattgtagt
     5701 attcctcgac ggtgattact cggactatcc ggaagagctg cctatactcg taaagccgat
     5761 tatcgaggac ggatacgaca tgataatagg ttcgaggaca aggggcgagc gggaaagggg
     5821 cgctctcctc ccgcaggcgt ttttcgggaa cgcgctcgcg acgaccctta taaaatggat
     5881 ttacggagtc gagttcacgg atctgggccc attccgcgcg ataaggttcg acaagctgct
     5941 cttgctcggg atgacagaca tgacgtacgg ctggacagtc gagatgcaga taaaggccgc
     6001 caagcaggga ctcaggtgcg gagaggtccc cgcgagctac aggaagcgca tcggagaatc
     6061 gaaagtgacg gggactctcg ggggcacggt catggcgggc tacaaaattc tcttcactat
     6121 attcaagcac atttggtgac cgcgacacca acaaaagcag gtcgtccttt tccgcccgga
     6181 cgatttcagt agataaaatc tatcaggctc cctatgcgga gccataacct cgcgagcgcg
     6241 ttcagtatga aattctcccc cgtgaacacg gccacgctca ccgacgcccc gatcctgagc
     6301 ttgtatttgt cgttgtcgat gtcggtgatc ctgagacgca cgggaaaacg ctgcgcaagc
     6361 tgcacccagt tctcggggtc ctttacccgt ggaagaaaac ccgagggcac cccctgcccc
     6421 gtgctcacgc cccagtcggt gctttcgacg acagccttga atatcgtccc cggatagagc
     6481 gcgaggctca cctccgcctc ctgacccttc cttatctgtc ccacgctgtt ctccctgaaa
     6541 ttagcgacga cccaccagct gttgtcgtcg acgaacgtga gcaccgcgtc gccgacttta
     6601 atatacgacc cgacagtaag ctggaggttc gttatgtacc cgtccgtagg agcatagaac
     6661 gtcgtctgcg aaaggttgta ttccgccgtg gcgaggctgg cctcgacctc ctttataatc
     6721 gcgtaatcgc cgtctatctt gtattcgagt gcctgcttgg ccttctcaaa atcggccttc
     6781 gactggttca atttggcgag cgtcgagtta agctgttcta ctgactggtc gaattcgagc
     6841 ttggctatgt agttcttctc gacgagcggc ttcaggtcgt tgtaccgctg ggacgcgtat
     6901 gcgtgatcgg cctgtgattg tttcacggcc tgcccggcag ccgctatatc gcttttcaat
     6961 tcgtcgatgt tctgcctcgt ctggacgagc ttcgccttta agtgctgcac ctggtattcg
     7021 taaggtctcg ggtcgagact gaaaagcttc cgacccttct caacatactc gttattcttt
     7081 atatatacgt ggtctacctg cccgtccacc tgcggagcaa cttgaaggac gtaggcctgc
     7141 acataggcgt cgctagtata aggtgtgtac ctgtcgagga gtatatagta gaagatgagg
     7201 aggacgatga gcgagactat gaatataacc cagaacttcg ctgagtaggt catattaccg
     7261 ggtttcaatc tggagtacag gtctttaacg ctcatggaac cgtcgcagcg gaaacgacga
     7321 cgaccgaccg atcggaattt tccggagccg tcgccggatt accattttta atcagacatc
     7381 ggaggaggca cgccctcacc agccccaggg gcctcccgcc cagccccata gcccgggacc
     7441 gaactcggcc tgctcggcca tcatcgcggc ttcctgctcc tcgtttacga ggttggattg
     7501 gtacaccaat ctctcgtacc ggtcgtaggc cgcctgatca cccgtccaga tacatttaca
     7561 gttatacgga tccgcgtacc agtaaagcgg cttgttgtcc ctgacgctga actgtacctt
     7621 gttcggggtt agggagttta gcatctgttc ctgctccggt gtatctgcgg tgactattat
     7681 gaaccctgcc gcggccagca tcttatccgt gcttgcagcc tgttcgccct gaatcgccgc
     7741 acatccgctt atagtcaata atacggcgaa tgcggttagt gccaatctga tacgaaacat
     7801 tagattgctc ctccattacg atctcgctga aataattgcg ggaatgcgct tctcccggca
     7861 cccagcggac agactaaaac taacacctaa atcgaaataa ttaaatagcc ctcgatcatt
     7921 cggagttcgt acggcgactg ttccatttaa gcgcgttctc gagcgtgaga ccctggacgg
     7981 ttatcgaaaa cacgacgacg atgtacgtca tcagcacgat caggtcctgt tgcggggagt
     8041 cagcaagcga gagagccagg gctatagaga cccctcccct tatcccgctc cacgtcatta
     8101 tgaatcccga gagcgacggc agtcttctga acagctttaa aaatacgagc gggagctcca
     8161 cgcttatata ccgcgcggcc gtgatgagcg gcaccgccag gagcccgcag attatataag
     8221 gcctgctcag atgaaggacc aagacctcga gcccgatcag cacgaacagg atcgcgttga
     8281 gtacatcgtc tatgacctcc cagaggctcc tcatgtaggc tcttatatgt tcggcggatt
     8341 cgggcatctg cctccgcctc cttgcgagaa agagccccgc cacgacgcaa gtcagcgggg
     8401 ctgacacgtc gagcgtttcg gccaggaaat acccgcccga cacgagcgag agggttatca
     8461 acgctaccac gaccctgccg tcctggctcc gcacgactat gatgtatatc atcctgtatg
     8521 cgaggtatcc gagcccgatg ccgagagcta gacctcctag ggcctctttg aggaagaact
     8581 cccctatgtg aagtgcggtg acctgagtct ctcccgtctg tatctcgaca aagagaacaa
     8641 aaagcacgac cgcgacggcg tcgttgaaga gggactctcc cgatatcacc gattcgagag
     8701 tcttgccggg atggatgcgt ctgaagagct tgagcacgat cacggcgtct atgggggaga
     8761 tgatggctcc gaatacgaga gcgtatatga gaggcatgtc cacccctatc gcattgtaca
     8821 cccgccatac gatgaagccg gtaaggacag tggcgataac cattccaagc gtggccaggt
     8881 acgagatcgt ccacttgtat tcgccgagct cgtctatatt cacgctcgac gctccagcga
     8941 agaggagaaa gctcagcatg ccgttcatga agaggtggtc gaaatcggcc gcctgcacga
     9001 gctcgaccgc ccagtccgtt ctgccgaaac ccagataccc gagtgtgagt atcaggatcg
     9061 acgccaggag ggacgccagc atcacgccga tcgtggtggg gagcttgacg aacgcctcgt
     9121 tcagaaaact gaagaccgcc gtgactgtta tgagtatagt gaatacagcg aacaggttca
     9181 tctcgttatt taagaccggt cgtacggtca aagcacagct gcggagat
//
LOCUS       DASSJH010000219         7522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_271411, whole genome shotgun sequence.
ACCESSION   DASSJH010000219 DASSJH010000000
VERSION     DASSJH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271411"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VFJ67_04720"
     CDS             complement(<1..1028)
                     /locus_tag="VFJ67_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7289723.1"
                     /translation="MTISKTGLVIIVFVVLGLTLNACKQDQAQPPPPPQVTVSKPIVK
                     EIVEWDEYTGRLEAVDTVDVRARVSGYLESIHFKDGQTVKEGDLLFVIDPRPYQAELD
                     RAEAELRLAQARLSLAENDLKRAQHLVSARAISEEEADTKASDEQVARATVEQARAVV
                     NAAKLNVEFTQVRAPISGLISRKNVTVGNLINGGTGGTLLTTIVSLDPIYCYFEADEQ
                     SYLRYTSLEQEGKLPDQRHAQNPAYMELSNETGYPHKGYVDFIDNRLDPNTGTIRGRG
                     VFPNPGNTLTPGLFARMKITGSAEYKAILVPDEAVGSDQSQKYVMVVNPENTVEYRQV
                     TLGPRVNGL"
     gene            1371..1643
                     /locus_tag="VFJ67_04725"
     CDS             1371..1643
                     /locus_tag="VFJ67_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289724.1"
                     /translation="MTGSKTRIPTMEELSEALAAAGSAHHEYEQTVLHGVRHEEWAVF
                     YAPYVLGRLGDFVQPSVLIGLLTEAPLTDDWAESAAAHVLAELGRK"
     regulatory      1775..1859
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1967..2404
                     /locus_tag="VFJ67_04730"
     CDS             1967..2404
                     /locus_tag="VFJ67_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289725.1"
                     /translation="MARRRHIPKASMRRIGEAGIDFTGLKSSSVIGEKKNYNFQPEVT
                     EYNLWPFSTIEWVAAEGKYRARAMVQHKTTHNLVAFNSMHQDFGDAERPIDLLRHSLQ
                     GTAERPVYNIFGETIEVHTADIGGGAIVTVTEVLALIWSMTES"
     gene            complement(2527..2742)
                     /locus_tag="VFJ67_04735"
     CDS             complement(2527..2742)
                     /locus_tag="VFJ67_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET7289726.1"
                     /translation="MKPSNIDRLVFRIAGIFVLASLTLGYFVHPAWFLFTAFVGLNMF
                     QASFTGFCPMAKILKKTGVRAGEAFSE"
     gene            complement(2845..3540)
                     /locus_tag="VFJ67_04740"
     CDS             complement(2845..3540)
                     /locus_tag="VFJ67_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET7289727.1"
                     /translation="MYVSHKERHKTQRIGWLRAAVLGANDGIVSTASLVVGVAASNAT
                     HNSVLVAGVAALVAGALSMAAGEYVSVSSQADTEQADLSRERRELATDIESERDELTS
                     IYIGRGLDPVLAREVALKLMEKDPLTAHARDELGITETAAARPIQAALTSAATFAAGA
                     ALPLITAVFVPVSAAVPVVSGTSLVFLAALGALAAHTGGAPIFRGAARVTFWGALAMG
                     ITAVVGSLFGAAV"
     gene            complement(3665..4198)
                     /locus_tag="VFJ67_04745"
     CDS             complement(3665..4198)
                     /locus_tag="VFJ67_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7289728.1"
                     /translation="MAENPNPAFILYTACSVDGFIARENGSIDWLSKWDPAAAGYGEF
                     FGTIDGLIMGARTYDQILDTGKWPYDHKPCLVLSNRARKPFRECVEFFTGRVEDAVDL
                     MREWNLKRVWIVGGASMFTQFHRKKLIDDYIIAIIPIILGSGIPLIQSCDVEVSLRLV
                     SSKAQDSGLVLNHYKKI"
     gene            4398..5000
                     /locus_tag="VFJ67_04750"
     CDS             4398..5000
                     /locus_tag="VFJ67_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289729.1"
                     /translation="MRKYMFVIPFIFLALGCNDGGGGENRNFCYLPDASDPCFDENEG
                     GSTTIVTDQTVDEVLAEEEGMPGFEDELEIDDILNGLVIPRLGEPNCPFEQSELVIKT
                     AEEWNRFRNSCFFGMFDLPDVDFSESMVLVSTQNFAEFGTTIEAVLEFDNKLIAVVED
                     EVPTVPPPGPGFPFDIVSIPRRDLPVDFIRVEIPVGPVVE"
     gene            complement(5088..5246)
                     /locus_tag="VFJ67_04755"
     CDS             complement(5088..5246)
                     /locus_tag="VFJ67_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289730.1"
                     /translation="MREDIDIRLLDYFYFSHSAVEKANSLVVIEEESETGIKESEEKR
                     ASESNDKY"
     gene            5647..6096
                     /locus_tag="VFJ67_04760"
     CDS             5647..6096
                     /locus_tag="VFJ67_04760"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7289731.1"
                     /translation="MKLLTCTNAALLNISLLLLRLAVGIILFVAGAGKVLGWFGGMGM
                     DATINIFVTNMGIHPLLAYLSCYTEFIGGFLLIVGLLTRPAAFAITINMLVAGIVTLP
                     NGFMTGASYPFSMMVSSLVILLAGPMAYSLDAWLLSGGKKSSGAIVG"
     gene            6160..6585
                     /locus_tag="VFJ67_04765"
     CDS             6160..6585
                     /locus_tag="VFJ67_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7289732.1"
                     /translation="MAEKKYTGSCHCGSVRFECEIDLARGTSKCNCSICSKTRYWKAL
                     IKSDAFRLLRGEEYLTDYQFGSDTIHHLFCRRCGVKPFGRAHLDVTFGGETLSGVYYA
                     VNLACLDDVTPEELAEAPVRYEDGKNNNWESRPEEVRHL"
     gene            6675..7166
                     /locus_tag="VFJ67_04770"
     CDS             6675..7166
                     /locus_tag="VFJ67_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7289733.1"
                     /translation="MSEINDSMLEFLNGRHYATLATLNEDGSIHLTPVWYLFDDGLFY
                     VETSPSSRKARNVLARPQASIVVDSRRKQGDERWVSASGTAEIISGKRFEEIAAKILT
                     RYITKTGLEDPRVGPGFSAGGGIAICLTPASWGSWDFKSLDDQYFGSILRQTPEKWFH
                     QVD"
     gene            7352..>7522
                     /locus_tag="VFJ67_04775"
     CDS             7352..>7522
                     /locus_tag="VFJ67_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7289734.1"
                     /translation="MDNFATKFRAKDLDGIMSMYAPDIVSFDAIAQLQFKGADAYRKH
                     WETCLSFCQGHVR"
BASE COUNT         1702 a         1960 c         2078 g         1782 t
ORIGIN      
        1 aggccgttca ctctgggccc gagcgtgacc tgccggtact cgacagtgtt ttcggggttg
       61 accaccatca cgtatttctg cgactggtcg ctgccgaccg cttcgtcggg cacgaggatg
      121 gccttgtact ccgcgctgcc ggttatcttc atgcgggcga agagccccgg cgtgagcgtg
      181 ttgcctgggt tggggaatac gccccttccc cttatggtcc ccgtgttggg gtcgagccgg
      241 ttgtctatga agtccacgta gcctttgtgc gggtaccccg tctcgttcga gagctccatg
      301 taggcggggt tctgcgcgtg tctctggtcg ggcagcttgc cctcctgttc gaggctcgtg
      361 tatctgagat aggactgctc gtcggcctcg aagtagcagt aaatcgggtc gagcgaaacg
      421 atcgtggtca gaagcgtgcc gcccgtgccg ccgttaatca ggttccctac cgtcacgttc
      481 ttcctgctga tgaggcccga tataggagcc cgcacctgcg tgaactcgac gttgagcttg
      541 gctgcgttta cgacggcccg cgcctgctcg actgttgcgc gtgcgacctg ttcgtcggac
      601 gccttggtgt cggcctcttc ctctgatatc gcccgcgcgc tcacgaggtg ctgcgcgcgc
      661 ttgaggtcgt tctcggcgag cgagagccgc gcctgggcga gcctgagctc ggcctctgcc
      721 cggtcaagct ccgcctggta aggtctcggg tcgatcacga agagcaggtc gccctccttc
      781 accgtctgtc cgtccttgaa atgaatggat tcaagataac cgctcacgcg cgccctcacg
      841 tccactgtat cgacggcttc gagacgcccg gtgtattcgt cccactccac gatttccttt
      901 acgatgggtt tgctaaccgt tacctggggt ggagggggcg gttgggcctg atcctgtttg
      961 catgcgttaa gcgttaaacc cagaacaacg aaaacgatta ttaccagacc ggtctttgaa
     1021 atagtcatct gcatcgcgtc cttttgttat actttaaggt tattcttgct atggtttttc
     1081 cgatattact caataattac ggcatattgt aagtaagtac ttacttacat agaaattacc
     1141 agcccgaatt cggagtgtca agacagtcac gaatcttagg agccgtgcgg cggaagccgc
     1201 aaccgggaac ccgacgcctc ccggaacggt attgcacgca gcactttatg tgctttataa
     1261 attacgatgc tgcccggcaa aaagagattc cggccggtcc cgaattcgtg aaattagact
     1321 cttgctatat aaatttcaaa ttagtgactg ttcctgctat aatttatccc gtgaccggat
     1381 caaagacccg tatcccgacc atggaagagc tctcggaagc gctcgccgcc gcgggctcgg
     1441 cgcatcacga gtatgagcag accgtcctcc acggggtgcg gcacgaggag tgggccgtat
     1501 tctacgcccc gtatgtcctc ggaaggctgg gggatttcgt tcagccgagt gttctgatcg
     1561 gattactcac agaagcgccg ctcacggacg actgggcgga atccgcggcc gctcatgtcc
     1621 ttgcggaatt gggacgcaaa taaggacggt acaaatgaaa gtcgtcggta ttgaccactg
     1681 cccccgcata tacttcaatc tattcgactc ctttaattat tatcggctct ctaatcgatt
     1741 gacatcgaaa cgggatttgt attagaataa tttataacta gaagggcaaa ccatttgaaa
     1801 gaatggggcg caaagtcaca ggcctaagaa ctccggttta cggcggctgg attgctatag
     1861 tgggctcgaa taagccaatc tccctctgcg attcccctgt ataattccct ttgccccctc
     1921 tctactcacg tgaatgtcct tcaataattc cgtaaggggg ccggcaatgg cacgtaggag
     1981 acatatacca aaagcatcaa tgaggaggat aggtgaagct ggtatcgatt ttaccggcct
     2041 gaaatcatct tccgtgatag gggagaagaa gaattataac ttccagcccg aagtgacgga
     2101 gtataatctg tggccgtttt cgactatcga atgggttgcg gcggaaggga aatacagggc
     2161 gcgggctatg gtccaacaca aaacaaccca caacttggtc gcgttcaatt ctatgcacca
     2221 ggatttcggc gacgcggagc gtcccataga cctgttgagg catagccttc agggaacggc
     2281 tgagaggccg gtatacaata tcttcggaga aacgattgaa gtccacacgg ctgatatagg
     2341 cggaggtgcg atagtcactg tgacggaggt tctagcgctt atatggtcaa tgaccgaaag
     2401 ctgaggtgcg cgcggcaaac gtatcgggat acggtttcaa cgccggttgc ggaaagagcg
     2461 tccggtatca ggaacagctt ttgaattcgc atccctggtt cgctgatatt tatcgttctt
     2521 gctgaatcac tcggagaacg cttctccggc cctgactcct gttttcttga gtatcttggc
     2581 catcgggcag aaccctgtga acgaggcctg aaacatgttg agaccgacga aggccgtgaa
     2641 caggaaccac gccgggtgta cgaagtatcc gagcgtgagg ctcgcaagga cgaatattcc
     2701 ggcgatacgg aaaacgaggc ggtctatgtt ggacggtttc atgatttctc ccctccggaa
     2761 agttatacat ccgggattag ttattacacc tgacgcactt aaatatagga tacctcatat
     2821 gaaagactga aaaaaccgct catatcagac ggctgctccg aagagggagc ccactacggc
     2881 ggtgatgccc attgccagag cgccccagaa cgtcacgcgg gcagctcctc tgaatatcgg
     2941 cgcaccgccc gtatgcgctg caagcgctcc cagcgccgcc aggaacacga gagaagtgcc
     3001 cgatacgacc gggacggcgg ctgagacagg gacgaaaacg gccgttatca gtggtagcgc
     3061 cgcgccagct gcgaaagtcg cggcggaagt cagggcagcc tgtatcggcc gggcggccgc
     3121 agtttcagtt ataccgagct cgtcccgagc gtgcgccgtg agggggtcct tctccatgag
     3181 cttgagcgcg acctccctcg cgagcacggg atcgagaccc cgcccgatgt atatggacgt
     3241 gagctcgtcg cgctcgctct ctatgtccgt cgcgagctcg cggcgctcgc gcgagaggtc
     3301 agcctgttcc gtgtccgcct gcgagcttac ggatacgtat tcgcccgcgg ccatggagag
     3361 agcgcccgcg acgagggccg ccacacccgc gacgagcacg ctgttgtggg tcgcgttcga
     3421 cgccgcgacc cctacgacga gactcgcggt ggatactatg ccgtcgttcg cgccgaggac
     3481 cgctgccctc agccatccta tgcgctgtgt cttgtgtctt tccttgtggc ttacgtacat
     3541 aacagctccc ggaacaaata tatatgtacg attataggag cacgcgtagt ctttggcaat
     3601 cagggaaatc cgttcgggca attcactctt aggcgatccc ttgcggagga gccggcacgg
     3661 gaaattaaat ttttttatag tgattgagta ctagacccga gtcctgagcc ttgctgctga
     3721 ctaatcttag gctcacctcg acgtcgcagg attgtatgag aggtatcccc gagcctagta
     3781 ttatgggtat gatcgcgatt atatagtcgt cgatgagctt cttcctgtga aactgggtga
     3841 acatgctcgc cccgccgaca atccaaaccc gcttcaggtt ccattcccgc ataaggtcga
     3901 ccgcatcttc caccctgccg gtaaaaaact ccacacattc acggaaaggc ttccgggccc
     3961 tgttcgagag gacgaggcat ggtttgtgat cgtacggcca tttgcccgtg tccagtatct
     4021 gatcgtaagt cctggctccc attatgaggc cgtcgatcgt cccgaagaac tcgccgtacc
     4081 ccgccgcggc cgggtcccat ttggacagcc agtcgatcga gccgttctcc ctggctatga
     4141 agccgtccac gctgcaggct gtgtacaaaa taaatgccgg gttcgggttt tctgccataa
     4201 tctcacactc agctttatca tccccgcgga tgttatcgaa ggaacaaaca atatcataac
     4261 atattcgaat tgtttttaac cggtgccccc ttaccgtacg gtattgatta ttcggatagc
     4321 ataaataaaa ctttaatgat tttctttatt tcggaaacgg aatattattt gaaacccaac
     4381 tccggaggag acccgatatg agaaaataca tgttcgtcat tccgttcatt tttttagcgc
     4441 tcggctgtaa cgacggcggc gggggtgaaa accggaattt ctgttatctg ccggatgcgt
     4501 cggacccgtg cttcgatgag aatgaaggag ggagcacgac gatagtaacc gaccagacgg
     4561 tggatgaagt cctcgctgaa gaggaaggaa tgccggggtt cgaggacgag ctcgaaatcg
     4621 acgacatctt aaacggtctt gtaatacctc gtctgggtga gccaaattgt cctttcgaac
     4681 agagtgagct ggtaattaaa acggccgagg aatggaacag gttcaggaac agctgtttct
     4741 tcggcatgtt cgatctcccg gacgtggatt tctccgagag catggtcctc gtctcgactc
     4801 agaatttcgc cgagttcggc actacgatcg aagcggtcct cgagttcgat aacaaattga
     4861 ttgcggttgt tgaagacgag gttcctacgg taccaccgcc agggccgggc ttccctttcg
     4921 atatagtttc gataccgaga cgcgatctgc ccgtcgattt tatcagagtt gaaattccgg
     4981 taggtccggt tgttgagtaa ttggatgaag aaatttattg cgtcctgttt ctgttctttt
     5041 cagaaagtta cgccgggact ggaaataact gttaacaagc attcgctcta atacttatcg
     5101 ttagattcag aagcacgctt ctcttcggac tctttaatgc cggtttcaga ttcttcttcg
     5161 attacgacaa ggctgttggc cttttcgacc gcggagtgac tgaaatagaa gtagtcaagg
     5221 agtcttatat ctatgtcttc cctcacctaa atcacctccc ctgcctacat atgtatttac
     5281 attctaacat ttattgacag gcaatttaag tgttttttta atccggtatg cctgagcctc
     5341 cggatggccg ttaaaaagtt attaagcaga aaagaaatat cgattaaact gtcctatata
     5401 cgaaagcccg ttcgtcatat gtatgaagtt gaatctattt acggaggaaa tcatcatgaa
     5461 ttacaagatg gaacatacgg gagtcgaacg ggagctccac atgagccgcg tcctcgacgc
     5521 atggctggcg aatgccggga atgacggcgc tcaatacgga tagggcattc ggagggccgg
     5581 ggcgatcggt atcgttaact cggattagac aagagaaact aatttctgta accggaggat
     5641 atattgatga aactgttgac gtgcacgaac gccgctttgc tcaatatctc tctcttattg
     5701 cttcggctgg ccgtggggat catactcttc gtcgcgggcg ccggaaaggt cttgggatgg
     5761 ttcggcggaa tggggatgga tgccactata aatatcttcg tcactaatat gggcattcac
     5821 ccgctcctcg cttaccttag ctgttacacg gagttcatcg gtggatttct cctcatcgtg
     5881 ggcctcctca cccggcccgc tgccttcgcc ataacgatca atatgctcgt ggccgggatc
     5941 gtcactctcc cgaacggctt catgacggga gcctcgtatc ctttcagcat gatggtgagc
     6001 tctcttgtca tacttctcgc agggcctatg gcgtacagtc tggacgcgtg gctcttgagc
     6061 ggggggaaga agagctcggg cgcgatagtc ggttgatgcc ggcataatag aggggaagcg
     6121 ggatgaaaat aataatttta aattggacgg taacgctcga tggctgaaaa gaaatatacc
     6181 gggagctgcc actgcggctc agtccgcttc gaatgcgaga tcgatctggc gcgtggtaca
     6241 agcaagtgca actgctcgat atgctccaag accaggtatt ggaaagccct catcaaatcg
     6301 gacgcgttca ggctcctgcg gggcgaggaa tatcttacgg actaccagtt cggcagcgat
     6361 acgatccacc acctgttctg ccgccgctgc ggcgtaaagc cgttcggccg ggcgcacctt
     6421 gacgttacct tcggcggcga gaccctgtcg ggtgtctact acgcggtaaa cctcgcttgc
     6481 ctcgacgacg taactcctga ggagctcgcg gaggcgcccg tacgctacga ggacggaaag
     6541 aataataatt gggaatcccg gccggaagag gtcaggcatt tatgaggaga ggctagggca
     6601 gtcgcgggga cgttcggagt gcgctccccg ggcatacgat aaagcatatt aaatccgata
     6661 tcgggaggac aaccgtgtct gaaataaacg attcgatgct tgagttcctt aacggccgtc
     6721 attacgccac tctggcgaca ctcaatgagg acggctcgat acacctcacg cctgtgtggt
     6781 acctctttga tgacgggctt ttttacgtag agacgtcccc ctcatcgcgc aaagccagga
     6841 acgttctcgc ccgtccgcag gcttctatcg tggtcgacag caggaggaag cagggggacg
     6901 agcgctgggt gagcgcttcc gggacggcag agattatcag cggaaagcgg ttcgaggaga
     6961 tcgccgcgaa aatccttacg cgttatatca caaagaccgg tctcgaggac ccgcgcgttg
     7021 ggcccgggtt ttcagcggga ggcgggatcg ctatctgtct cacgccggcg agctggggat
     7081 cgtgggattt caaaagcctc gacgatcaat atttcggcag catcctgaga cagactccag
     7141 agaagtggtt tcatcaggtg gattaaaatc gggatctcta ctcagcatta attgggcaaa
     7201 tgattctgac tcatttgtcc gggcggtaaa agaatattaa tgaagtgtcc cgaatgcgcc
     7261 tccccgttcg tcgtatagat ataaacgaga aaaaaggaga tcgatcagga gcacgagcaa
     7321 aactaatgat gaaacacaga tacggcagct attggacaat tttgcaacga aattccgcgc
     7381 caaagacctg gacggcatca tgtccatgta tgcgcctgac atcgtgtcgt tcgacgccat
     7441 agcgcagctg cagttcaagg gagcagatgc gtacagaaag cactgggaaa cctgtctttc
     7501 cttttgtcag ggccatgtcc gg
//
LOCUS       DASSJH010000220         3980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_271441, whole genome shotgun sequence.
ACCESSION   DASSJH010000220 DASSJH010000000
VERSION     DASSJH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271441"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..842
                     /locus_tag="VFJ67_04780"
     CDS             111..842
                     /locus_tag="VFJ67_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET7289735.1"
                     /translation="MKKMSLDIERTGLDISEKGRAKDGTELSSDRRLLMQLLAFGNCH
                     NPRKLVDELKKSKMDFALYADINDPYGIGLLAVNEDPDFFVGGLREFLNGSAFTELTP
                     KPEYTMLGRTYSFGYEPDLERTLIHGPRERVLDPELKWAIWYPLRRNKLFETLSSEEQ
                     RSVLGEHGKLGFKFGDAGYGKDIRLACHGLDKNDNDFVIGVLGKKLYPLSILIQSMRK
                     TKQTSMYLESLGPFFIGKVLWQAKA"
     gene            complement(901..2232)
                     /gene="hslU"
                     /locus_tag="VFJ67_04785"
     CDS             complement(901..2232)
                     /gene="hslU"
                     /locus_tag="VFJ67_04785"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET7289736.1"
                     /translation="MSSETFFTPREIVSELDRYIIGQNKAKRAVSIALRNRWRRQRVS
                     PELKDEIAPKNILMIGPTGVGKTEIARRLAKLAQAPFLKVEASKFTEVGYVGRDVESM
                     IRDLTDIAIKMITDEEKQTVRAKAEDLAEERMLDLLFPPVGGEQPHTETGGETREKLR
                     KLLREGRLDERFVDIEVTSKNFPIQVFSPQGLEEMDVNISDMLGNLFPKKTKKRKVRV
                     PDAMEVLIQEEAQKLIDMDNVVKMAIERVEQSGIIFIDEIDKVAGRESTAGPDVSREG
                     VQRDLLPIIEGCTVNTKHGMVRTDHILFIGAGAFHVSKPSDLIPELQGRFPIRVELEP
                     LTTEDFINILTQPKNALIKQYTELLNTEKIDLIFKEDAIKEIAETAQTVNESTENIGA
                     RRLHTIMERMLDEISFNAPDMAEQKIVIDAKYVKEKIADIVKDRDLSRYIL"
     gene            complement(2234..2770)
                     /gene="hslV"
                     /locus_tag="VFJ67_04790"
     CDS             complement(2234..2770)
                     /gene="hslV"
                     /locus_tag="VFJ67_04790"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET7289737.1"
                     /translation="MENFHGTTIVCVKIGERVAMAGDGQVTLGHAAVKHSAKKVRKIF
                     EGKVLVGFSGATADAMTLFDKFEAKLEEFRGNLRRAAVELARDWRTDRILRRLEALLA
                     VADKDSMFLISGSGDVLEPDDNVVAVGSGGSYAQAAAKALSRHSGLGAREIAEESLKI
                     ASEICIYTNDKITVEELS"
     gene            complement(2773..3666)
                     /gene="xerC"
                     /locus_tag="VFJ67_04795"
     CDS             complement(2773..3666)
                     /gene="xerC"
                     /locus_tag="VFJ67_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET7289738.1"
                     /translation="MEQLIQNFRDYLGSERNYSKHTVKAYIHDIGEFSRVIKEKKLAS
                     GKDGSTDFGRLDETSVRAYISWLYTRNSRTSISRKLASVRTFFEFLVRKGTVKSNLAK
                     LVPTPRGEKRLPTFLTVDEVVKLLSTKVSDEYSTRDIALLELLYSSGLRVSELVGIDL
                     NDIDLKNLTVKVMGKGGKERIVPVGSKAAEALNKYLRERPDMNPTGDHLFVNTRGDRL
                     NVRSVDRVIRKYAALAGIPKNVSPHVLRHTFATHLLGGGADLRAIQEMLGHKSLSTTQ
                     RYTHTSIEKLMEIYDKTHPRA"
BASE COUNT          749 a         1146 c         1024 g         1061 t
ORIGIN      
        1 atgcaaataa attcttttta ttgcgcggat aattggcggg agcgggcgca aatgatcccc
       61 ttcaatcgcc aaccgcccgt ttccctgata tatttaataa ccgcggaggt atgaagaaaa
      121 tgtctctcga tattgaacgc acgggtctcg atataagcga gaagggtagg gcgaaggacg
      181 gaacagagct ctcctcggac agaaggctgc tcatgcagct cctcgccttc ggaaactgcc
      241 ataacccccg gaaattagtc gatgaattga aaaagtctaa gatggatttt gccctttacg
      301 ccgacataaa cgacccctac ggaataggtc tcctggccgt gaacgaggac cccgatttct
      361 tcgtcggcgg gttgagggag ttcctgaacg gctcggcatt cacggagctt acgcccaagc
      421 ccgaatacac gatgctcggc aggacttact cgttcgggta cgagcctgat ctggaaagga
      481 ctctcataca tgggcctcgc gagagagtac tcgaccctga gcttaagtgg gcgatctggt
      541 atcccctcag gaggaacaag ctcttcgaga cgctttcctc agaggagcag cgctcggtgc
      601 tcggcgagca tgggaagctt ggattcaaat tcggggacgc cgggtacggc aaggacatac
      661 gcctcgcctg tcacggactc gacaagaacg acaacgactt cgttatcggg gtcctcggga
      721 agaaactcta tcccctgtcc atactcattc aatcgatgcg gaagacgaag cagacctcga
      781 tgtatctcga aagcctcggg ccttttttca tcggaaaggt actctggcaa gcgaaagcgt
      841 aattcgctcg agactgcgcg ggcgggactc actcttatgt gagtgcggca caggagccgt
      901 ttataagata taacggctga ggtccctgtc cttcactata tccgcaatct tttccttcac
      961 gtatttcgcg tctataacta tcttctgctc ggccatatcg ggcgcgttga aggaaatctc
     1021 atctaacatc cgctccatta tcgtatggag cctcctcgcg cctatgttct ccgttgactc
     1081 gttcactgtc tgagccgtct cggctatttc ctttatcgca tcttccttga atatcaggtc
     1141 tatcttttct gtgttgagaa gctcggtgta ctgttttata agtgcgttct tcggctgcgt
     1201 aagtatgttt atgaagtctt ccgtcgttag cggctcgagc tcgaccctta tcgggaacct
     1261 gccctgaagc tccggtatga ggtcggaggg tttggacacg tggaacgcgc cagcgcctat
     1321 gaatagtata tggtcagtcc tcaccatccc gtgcttcgtg ttcaccgtgc acccttcgat
     1381 tatcgggagc aggtccctct gcacgccttc tctcgatacg tcgggccccg cggtgctttc
     1441 gcgcccggcg actttatcga tctcgtcgat gaatattatc cccgactgct cgaccctctc
     1501 tatcgccatc ttcaccacgt tgtccatgtc gatgagcttc tgagcctcct cctgaatgag
     1561 cacctccatc gcgtcgggga ccctgacctt cctcttcttg gtcttcttcg gaaagaggtt
     1621 gccgagcatg tccgatatgt tgacgtccat ctcttctaat ccctgggggg agaagacctg
     1681 tatgggaaaa tttttggacg tcacttctat atctacgaac cgctcgtcga gcctcccttc
     1741 ccggaggagc ttcctcagct tctccctcgt ctcgccgccg gtctcggtat gcggctgctc
     1801 gccccctaca ggagggaaca ggaggtcgag catcctctcc tcggccaggt cttcggcttt
     1861 ggccctgacg gtctgtttct cctcgtccgt gatcatcttt atggctatgt ccgtaaggtc
     1921 cctgatcatc gactcgacgt ccctgccgac gtagcccact tcggtgaatt tagatgcctc
     1981 caccttgagg aacggggcct gtgcgagctt cgcgagcctc ctcgctatct cggtctttcc
     2041 gacccccgtg ggcccgatca tcagtatgtt cttgggggcg atctcgtcct ttagctcggg
     2101 agatacgcgc tggcgtctcc agcggttacg gagcgctatc gatacggctc gtttagcctt
     2161 gttctgccct attatgtacc tgtccaactc ggagactatc tccctcggag tgaaaaacgt
     2221 ttcgctgctc attttatgag agttcctcta ctgtgatttt gtcgttggta tagatgcaaa
     2281 tttcggaagc tatcttgagc gactcttccg cgatttccct cgcgcccagt ccggagtgcc
     2341 gggaaagcgc cttcgccgcc gcctgggcgt acgagcctcc cgagccgact gcgacgacgt
     2401 tgtcgtcggg ctcgagcacg tcaccgctcc ctgatatcag gaacatgctg tccttgtcgg
     2461 caaccgccag gagcgcctcg agcctcctca gtatgcggtc ggtcctccag tccctcgcga
     2521 gctctaccgc cgcccttctc aggttgcccc tgaactcctc gagcttcgcc tcgaacttgt
     2581 caaagagcgt cattgcgtcg gcggtcgctc ctgaaaaccc tactaagacc ttgccctcga
     2641 atatcttcct taccttcttg gccgagtgtt tgacggcggc gtgaccaagc gttacctggc
     2701 cgtcccctgc catcgctact ctctctccta tctttacgca tactatcgtc gttccgtgga
     2761 agttttccat gtttacgctc tcgggtgtgt tttatcgtag atttccatta atttttcaat
     2821 cgacgtatgt gtgtatctct gcgtggtgga aaggctcttg tgtccgagca tctcctgaat
     2881 ggccctcagg tcggcgcccc ctccgaggag gtgtgtggcg aacgtatgcc tgagaacgtg
     2941 cgggctcacg ttcttgggta tgcccgcgag cgcggcgtat ttccttatga ccctgtcgac
     3001 gctcctcacg ttgagcctgt ctcccctcgt attgacgaag aggtggtctc ccgtggggtt
     3061 catatccggt ctctctcgaa ggtatttatt cagagcctca gccgccttcg agcccaccgg
     3121 cacgatcctc tccttgccgc cctttcccat gaccttcacg gtgaggttct tgaggtcgat
     3181 gtcgtttaaa tcgattccga ccagctcgct caccctgagg ccgctcgagt aaaggagctc
     3241 gagaagtgct atgtcccggg ttgaatactc atccgagacc ttggtgctga gcagcttcac
     3301 gacttcatcg accgtaagga atgtcggaag cctcttctct ccgcgcggcg tggggacgag
     3361 cttcgcgagg ttgcttttaa ccgtcccttt tctgactagg aactcgaaga aggtccttac
     3421 cgacgcgagc ttcctcgata tggaagtcct cgaatttctc gtatagagcc agcttatata
     3481 tgccctcacg gacgtttcgt cgagcctgcc gaaatcggtc gagccgtctt tccccgacgc
     3541 gagctttttc tctttgatta cgcggctgaa ttcgcctata tcgtggatgt aggccttgac
     3601 ggtgtgtttg gaataattcc tttcggaacc cagataatcc ctgaaattct gtattagctg
     3661 ttccataacg gcaatcttta taatatatac acagctcccc ttctttcaat ggagagtgtt
     3721 tgtagggagc gggggaggct gaaagcgggt ccgtttgaaa taaccggctc cgggggcatg
     3781 ctgcctgtcg ccgatctggt tattttctat gagcgcggtt tttaattctc gaacccggcg
     3841 tagtccctgg aaagctcgtc atagaacaac cccgacagct gcacgcgttt cgcgcctatg
     3901 tagcgctttt tgtaatactg tttgttgatg ctgtcgatgg tgaccttcct tgaggccgaa
     3961 gaagcgtgta tgaactcgtc
//
LOCUS       DASSJH010000221         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_488172, whole genome shotgun sequence.
ACCESSION   DASSJH010000221 DASSJH010000000
VERSION     DASSJH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488172"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFJ67_04800"
     CDS             complement(<1..846)
                     /locus_tag="VFJ67_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289739.1"
                     /translation="MRREQIIEFLNFDDLVNRHPKDEPYVVLIGYESFQPTEYEEVAL
                     YSHSVHFNSLMISTDSKERALNEIDYHLVFASEGGYFEQAPSGEMIYNLNESWDYVDG
                     LTVEPLILDRHWPGLGIQFYEPTQRMILYLGALNKNGDWINPYTEDVIIRSMQKNELL
                     NEVRHSINRIEIKVDYLKDYLAARKSGLLIAKYSSRVLMFQSTDQIPILDSSKEVPHG
                     RWNYHYVNEDSKSPKELWAEAELRQKFWIDPLPEPRHEGAHPRGEFVGGVKFTLQDGT
                     KEEYDV"
     gene            complement(920..2014)
                     /locus_tag="VFJ67_04805"
     CDS             complement(920..2014)
                     /locus_tag="VFJ67_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7289740.1"
                     /translation="MNSNAWPEKARTFCDLIGLEHPIIQAPMAGGPTTPGLVAAVSNA
                     GGLGSFPGGYLSPDELEDAIRKIRSLTDKPFAVNLFVSEKPRKRKPPQKVMKKLAEIA
                     GEVGADLPDSIEGESFTFEDELGVILDNNVPVFSFTFGVPKRKHMNELRRRKITVIGT
                     ATCVREGIKLAEVGCSAVIAQGSEAGAHRGTFPETEGVPMVGGISLVPQMADNTGVPV
                     ICSGGIMDGRGIAAALALGASAVQMGTAFLGCHEAAVPAPWQEALRNSDDSSTVLTRV
                     YTGKYARGIRNRFYDEMSALEGKVPSYPIQNYLTRPIRNASRAKGDPEYMSLWAGQGS
                     PMMRNCAAGELVRMLVEEYGEAIERLCESF"
     gene            complement(2162..3472)
                     /locus_tag="VFJ67_04810"
     CDS             complement(2162..3472)
                     /locus_tag="VFJ67_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HET7289741.1"
                     /translation="MTDSESTTGTAAGEISGVVEHCFRHEAGKIVSTLTRKFGAKHLN
                     LAEDVVQEALARALQTWPYYGVPKNPAAWLTQVAKNLALDVVRRDKVFRNKEIDIANF
                     MENNPASPGGGDSVSFKDEIRDDSLWMIFMCCHPAVPREAQVALALKTICGFNAGEIA
                     KAFLTSEAAVLKRLTRAKRRIREASIPFELPAGDELGDRLDSVLDTVYLLFNEGYKAS
                     GGDRLTRSDLCGEAIRLCTLLAGHPLSDQPRVHALLALMLLNGSRLPARTDPDGNIML
                     LKDQDRALWDRAMIARGLFHLVQSAEGEELSVYHLQAGISACHCAAPDYESTDWRRIL
                     HYYDILTAIDDSPVAALNRAVAVAKVHGPEAGIEAVEAIPNRDQLETYYLLYAVLGEF
                     EELLGNRKAAAEYFRRSLDLVTIKSERKLLMKRIRAIESGRRGP"
     gene            complement(3489..>3716)
                     /locus_tag="VFJ67_04815"
     CDS             complement(3489..>3716)
                     /locus_tag="VFJ67_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7289742.1"
                     /translation="FAESKEAIAGYFLLKVDSLDEAVEIAKNYPGLNYGATVEVRPVA
                     PECAATLSLLEKTGEGGVTESVTQAGASRFS"
BASE COUNT          786 a         1082 c          899 g          949 t
ORIGIN      
        1 aacatcgtat tcttcttttg taccatcctg caaggtaaac ttaacacctc ctacaaactc
       61 tcctctagga tgagcacctt catgcctagg ttccgggagt ggatcaatcc aaaatttttg
      121 tcttaattct gcttccgccc agagctcctt aggggatttg gaatcctcat ttacataatg
      181 ataattccac cttccgtggg gaacctcctt gctactgtct aatattggga tttgatcagt
      241 tgattgaaac atcagaactc tcgatgaata tttagctatt aatagaccag actttcttgc
      301 agctagatag tctttcaagt aatcaacttt aatttcgatt ctattaattg aatggcggac
      361 ttcatttaac agctcgttct tctgcatact tcttataatt acgtcttcag tatatgggtt
      421 aatccaatct ccatttttgt tcaatgcacc caagtataga atcattcttt gagtaggctc
      481 ataaaattga attccaagac ctggccaatg acgatctagt attagtggtt ctacagtaag
      541 accatccaca taatcccaac tctcgttaag attataaatc atctcaccgg atggagcttg
      601 ctcaaaatat ccaccttcgg atgcaaacac taaatggtaa tcaatttcat ttagtgctct
      661 ttctttagag tcagttgaaa tcattaaaga attaaaatgt actgagtggc tataaagggc
      721 tacttcttca tattcagttg gttgaaatga ttcgtatccg attaacacaa cgtacggttc
      781 atctttagga tgtctattta caagatcatc aaaatttaag aactcaatta tttgctctct
      841 tctcatctcg atattttaga aacaagttat cactaattag tttattcact aaaaccacct
      901 ttgcaaatca aaacttcttt cagaaactct cgcacaaccg ttcaatcgct tccccgtatt
      961 cctcgaccag catcctcacg agctctcctg ccgcgcagtt tctcatcatc ggcgagccct
     1021 gccccgccca gagggacatg tactcggggt cgcccttggc cctcgacgca ttcctgatgg
     1081 gccgcgtgag atagttttgg atcggatacg aggggacttt cccttcgagc gccgacatct
     1141 cgtcgtaaaa gcggttcctt attcccctcg catacttccc cgtatatacc ctcgtcagca
     1201 ctgtgctcga atcgtctgaa ttccggagcg cctcctgcca cggcgcgggg accgccgctt
     1261 catggcatcc gaggaacgcc gtgcccattt gtaccgccga ggctccgaga gcgagcgcag
     1321 ccgctatccc ccgcccgtcc ataatcccac ccgagcatat gaccggcaca ccggtgttgt
     1381 ccgccatctg gggcacgagc gatatgccgc cgaccatggg aaccccctcg gtctcgggga
     1441 acgtacccct gtgcgccccg gcctcgctcc cctgagcaat tacggcgctg cacccgacct
     1501 cggcgagctt gattccttcc ctgacgcacg tggctgtgcc gatgaccgtg atttttctcc
     1561 tgcggagctc attcatgtgc ttacgcttgg gcacaccgaa cgtgaagctg aataccggga
     1621 cgttgttatc gagaataacc ccgagctcgt cttcgaaggt gaagctctcc ccttcgatcg
     1681 agtccgggag atcggcgccg acctctcccg cgatctccgc gagcttcttc atcacttttt
     1741 gcggaggctt cctcttgcgc ggtttttcgg acacgaaaag gttcacggcg aatggcttat
     1801 cggtcagaga gcgaatcttc ctgatcgcgt cttctaactc gtcgggcgaa aggtatcccc
     1861 ccgggaacga gcccagtccg ccggcgttgg agacggcggc cactaacccg ggcgtagtag
     1921 gcccgcccgc catgggagcc tgaataatcg ggtgctcaag tccgataaga tcgcagaatg
     1981 tccttgcctt ttccggccat gcatttgaat tcatgtttcc ccctgataac gcggctgaga
     2041 atgtcaattt tattttacat aatattaatc ggtcagaagg aaaagtgttt cccccggagt
     2101 agattcctta gaaattaact tttatgagtc ggaagccgaa gctccgcggc tccgatttga
     2161 actaagggcc cctgcggccg ctctctatgg cgcggatcct tttcatgagg agcttccttt
     2221 cggacttgat cgttacgagg tcgagcgatc tgcggaaata ttcggcggct gctttcctgt
     2281 tgccgagaag ctcttcgaac tcaccgagga cagcatagag gaggtaatag gtctcgagct
     2341 ggtcgcggtt gggtatggct tccacggctt ctatcccggc ttcggggccg tgaaccttgg
     2401 cgactgcgac agcgcggttg agcgcggcga ccggagaatc gtcaatggcc gtcaatatat
     2461 cgtaataatg caggatgcgc cgccagtcgg tcgattcgta atcaggagcc gcgcagtggc
     2521 aggccgagat gccggcctga agatgatata cgctcagctc ttcgccttcc gctgactgca
     2581 ccagatgaaa gagcccccgt gctatcatcg ccctgtccca tagcgccctg tcctggtctt
     2641 tgagcagcat gatgttcccg tcagggtccg tccgagccgg gagacgggag ccgttcagca
     2701 gcatgagggc cagaagcgcg tgcacgcggg gctgatcgct cagcgggtgt cccgcaagca
     2761 gtgtgcatag ccttatggcc tcgccgcaaa gatcgctcct tgtcaggcgg tctcctcccg
     2821 aagccttgta tccttcgttg aacagcagat aaacggtatc gagcaccgag tcgagcctgt
     2881 ctccgagctc atcccccgcg gggagctcga aaggtatcga ggcctcgcgt atcctgcgct
     2941 tcgccctcgt cagcctcttc agcacagccg cctccgatgt gaggaacgcc ttcgcgattt
     3001 cccccgcgtt gaacccgcaa atggtcttga gcgcgagcgc cacctgcgcc tcgcgcggga
     3061 ccgccggatg gcaacacatg aatatcatcc acaaactgtc gtccctgatc tcatccttga
     3121 atgacacgga atcccctcct cctggcgatg ccgggttatt ctccatgaaa ttcgctatat
     3181 cgatttcctt attgcggaat accttgtcgc gccttacgac gtcgagcgcc aggttcttcg
     3241 caacctgcgt gagccatgcg gcggggttct tcggtactcc gtaatagggc cacgtctgga
     3301 gcgcccgcgc gagcgcttcc tgtaccacgt cctcggcgag gttcaggtgt ttcgccccga
     3361 atttcctcgt cagggtggag acgatcttgc ccgcctcgtg gcggaaacaa tgctcgacga
     3421 cacccgatat ttccccggcc gccgttcctg tcgtgctttc ggaatcagtc atccgtctat
     3481 gctgccggtc aactaaaccg cgatgcaccg gcctgcgtaa cactctcggt aacaccgcct
     3541 tccccggttt tttcaaggag gctcagcgtg gcggcgcact cgggcgcgac tggccttacc
     3601 tctacagtcg ctccgtaatt caggcccgga tagtttttgg caatctcgac tgcttcgtcc
     3661 aggctgtcca cttttaatag gaaatatccg gcgatcgctt ccttggattc cgcaaa
//
LOCUS       DASSJH010000222         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1461597, whole genome shotgun sequence.
ACCESSION   DASSJH010000222 DASSJH010000000
VERSION     DASSJH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461597"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /gene="tatC"
                     /locus_tag="VFJ67_04820"
     CDS             complement(<1..629)
                     /gene="tatC"
                     /locus_tag="VFJ67_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7289743.1"
                     /translation="MEESKMSFLEHLGELRRRMLWSILAVLVCFIPAYYFSGQIFDFL
                     MEPIRNELPEGSMLIFTMPAEGFTTYLKVSIYTAIAAAVPFILYQVWKFVAPGLYKHE
                     KRIIIPFILFGTLFFALGGSFFYFVVAPKAFEFLLNEYSSDYVKAFPSIKEALSFFMA
                     LILGFGLIFEFPLVIFVLARIGIVSSAWLKRQRKYAILVSAVAAAVLTPT"
     gene            856..2442
                     /gene="serA"
                     /locus_tag="VFJ67_04825"
     CDS             856..2442
                     /gene="serA"
                     /locus_tag="VFJ67_04825"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET7289744.1"
                     /translation="MKVLITDGMSKEGLSILESAEGLDLDVRKSTPKEELAEIIGGYD
                     AIVIRSATKLTADLIEAGKNLTVIGRAGIGVDNVDVEAATKKGIVVMNTPEANAITTA
                     EHTITLMLSLARQIPQAHASLKAGKWERNKFKGMEIYGKTLGCIGLGNIGKLVAERAL
                     GLKMNVIAYDPFLSKEAAEKIGVELVSLDDLLKRSDIITIHTPLTPETKNLINKDTIE
                     KMKKRIVLINCARGGVINENDIAEAIKSGRVAGAAFDVFVNEPPEGGNPIIALEENVV
                     FTPHLGASTEEAQTKVGLAIAEQIVDFLKNGVVKNAVNMPSVSLEVLKTMKPYLNLAE
                     KLGSLQGQLCKGGVKEIHIEYDGEVSELDTSPITVSALKGFLTPMMDVVVSHVNAPVI
                     AGERGIKVIEAKSSQTKDYRSLISLRVITEQGESQVSGTIFGKEEPRFVRINGVTIDV
                     VPKGYLLVSENNDRPGFIGAMCTLLGENGVNIGLLHLGREAIGGRAIVFTNVDSPVPQ
                     NVIEKISKLPDIISVTQVNL"
     gene            2443..>3081
                     /locus_tag="VFJ67_04830"
     CDS             2443..>3081
                     /locus_tag="VFJ67_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET7289745.1"
                     /translation="MINRLSLPQGVKDYTPEKAEELRRVEDGLLEEFGRWGYRKIITP
                     LFEYLEPLSVGLGDELKSKVMKFVDPSSGEVVALRPDITPQVGRIVATQMKDHTAPLR
                     LCYNGRVVRFEEKGSGKEREIFQVGCELVGLSSPEADAEIIALGVKSLGKSGIKNLVL
                     DIGHTGILRYLLDRTGDLRESIEEALKKKDQEALSRAIAKSRAPKDIRDAILS"
BASE COUNT          868 a          778 c          877 g          558 t
ORIGIN      
        1 gtgggcgtga gcaccgcggc cgcgaccgcg ctcacgagta ttgcgtattt cctctgtctt
       61 ttgagccatg cgctcgatac gataccgatc ctcgcgagga cgaatataac gagagggaac
      121 tcgaatatga gaccgaagcc cagaatgagg gccatgaaga acgagagggc ctccttgatc
      181 gaggggaacg ctttcacata atcggaagaa tattcgttga gcaggaattc gaatgcctta
      241 ggcgcaacga cgaagtagaa aaacgaaccc ccgagcgcga aaaacagtgt cccgaaaaga
      301 ataaacggga ttattatccg cttttcatgc ttgtagagcc cgggcgcgac gaacttccag
      361 acctgataga gtatgaaggg cacggctgcg gcaatcgccg tataaatgga gactttgaga
      421 taggtcgtga agccctcggc cggcatcgta aaaataagca tgctcccctc gggcagctcg
      481 tttcttatag gctccataag gaaatcgaat atctgtcccg aaaaataata agcaggaatg
      541 aagcagacca gaacggccag gatcgaccag agcatcctcc gcctgagttc tcccaggtgt
      601 tcaaggaacg acattttgga ttcttccata gttgcggcaa aagattaact caatagcgtg
      661 cggttctcaa acatttgcat caggcgcgcg gattagagtc agcggaatac agtcataagt
      721 gccgtgccga atccccggac ccccagaatc gagtaacgtc ccgccggcta acctccgaaa
      781 ttgacaataa atcatagctc gcatatattg ttatcccgtt aaaaatcatc ccttatatat
      841 ctctcggagg aagaattgaa agtactgatt acagacggta tgtccaagga aggactcagc
      901 attctggagt ccgccgaagg tctcgacctg gacgtcagaa agagcacgcc taaggaagaa
      961 cttgcagaaa tcataggcgg ttacgacgct atagtaataa ggagcgcgac caagctcacg
     1021 gccgacctga tagaggccgg aaagaacctg acggtaatag gcagggccgg gataggcgtt
     1081 gataacgtgg acgtggaagc ggcgacgaag aaaggaatcg tcgtcatgaa caccccggag
     1141 gcgaacgcca tcaccaccgc cgagcacacg atcacgctca tgctctctct cgcgagacag
     1201 ataccgcagg cccacgcgtc gcttaaagcg ggcaagtggg aaaggaacaa gttcaaaggg
     1261 atggagatct acggcaagac cctcgggtgc atcggtctcg gaaacatagg aaagctggtc
     1321 gctgagaggg cgctcgggct taagatgaac gtaatagcct acgacccgtt cctgtccaag
     1381 gaggccgcgg agaagatcgg cgtggagctg gtttcgctcg acgacctcct caagaggtcg
     1441 gacataataa cgatacacac cccgctgacc cccgagacga agaaccttat caataaagac
     1501 acgatagaaa aaatgaagaa aaggatcgtg ctcattaact gcgcgcgagg cggagtgata
     1561 aatgaaaacg atatcgccga ggctataaaa tctggcaggg tcgcgggcgc cgctttcgac
     1621 gtgttcgtga acgagccgcc tgaagggggg aaccccataa tcgccctcga agagaacgtg
     1681 gtattcaccc cccacctcgg agcctcgacg gaagaggcgc agacgaaagt agggctcgct
     1741 atagccgagc agatagtcga tttccttaag aacggagtgg tgaagaacgc tgtcaacatg
     1801 ccctctgtga gcctcgaagt gcttaaaacg atgaagcctt acctcaacct tgccgagaag
     1861 ctcgggagcc tacagggcca gctctgcaaa ggcggggtca aggaaataca tatagaatac
     1921 gacggcgagg tctcggagct cgacacctca ccgataaccg tgtccgctct caaggggttc
     1981 ctcacgccca tgatggacgt ggtcgtgagc catgtaaacg cccccgttat cgccggggaa
     2041 aggggtataa aggttataga agcgaaatcc tcgcagacga aggactatag gagcctgata
     2101 tccttaaggg taataacgga acaaggcgaa agccaggtgt cgggcaccat attcgggaag
     2161 gaagagccca ggtttgtcag gataaacggt gttacaatag acgtcgtgcc caagggatac
     2221 ctcctcgtga gcgagaataa cgacaggccg ggattcattg gcgcgatgtg cacactgctc
     2281 ggagaaaacg gagtgaacat aggtctcctc cacctgggcc gcgaagcgat aggaggacgg
     2341 gccatcgtat ttacgaacgt ggattctccg gttccgcaaa acgtgataga aaaaatatca
     2401 aagctcccgg acatcatttc cgtgacacag gtcaatctat agatgataaa ccgccttagt
     2461 cttccacagg gtgtaaaaga ctacacgccc gagaaagcag aagagctcag aagggtagaa
     2521 gacggtcttc tcgaagagtt cggcaggtgg ggttacagga agataatcac cccgctcttc
     2581 gaatacctgg agccgttgtc cgtagggctc ggcgacgaac tcaagagcaa ggtcatgaaa
     2641 ttcgtcgacc cctcttcggg cgaagtcgtc gcgttgagac cggacataac gccgcaagtg
     2701 ggcaggatcg tcgccactca gatgaaggac cacaccgcgc ccttgaggct ctgctacaac
     2761 ggcagggtcg tcaggttcga ggaaaaaggg agcggcaagg agcgggaaat cttccaggtg
     2821 ggctgtgagc tcgtagggct atcttcgccc gaagccgacg ccgagatcat cgctctcggg
     2881 gtcaagtcac tcgggaaatc ggggatcaag aacctcgtgc tcgacatcgg gcacacgggc
     2941 atcctcagat acctgctaga caggacgggg gacctcagag agagcatcga agaggctctt
     3001 aaaaaaaaag accaggaagc gttgtcgagg gcgattgcca aatcgcgcgc tcctaaagac
     3061 ataagagacg cgatcctcag c
//
LOCUS       DASSJH010000223         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_55492, whole genome shotgun sequence.
ACCESSION   DASSJH010000223 DASSJH010000000
VERSION     DASSJH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55492"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFJ67_04835"
     CDS             complement(<1..362)
                     /locus_tag="VFJ67_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7289746.1"
                     /translation="MEAANKEYRLKKPILPPGPRGLPYIGSLAGYFSDPLGFLTRIAH
                     EYGDIVYFTLGSRRIYLLNNPEHIKDVLVTHNRNFTKSRALNRAKLVLGEGLLTSEGD
                     LHLRQRRIIQPVFHYRRI"
     gene            490..954
                     /locus_tag="VFJ67_04840"
     CDS             490..954
                     /locus_tag="VFJ67_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7289747.1"
                     /translation="MEDVKTLRRILRENKTIAVVGLSDKWHRPSYFAAKYLQEHGYRI
                     IPVNPGHKEILGEKCYASLLDIPDKVDVVDIFRKAEDVPPIVEDAIKIGAKVVWMQLG
                     IINEEAAKRAREAGLEVVMNRCMKIEHGRLFGGINFIGVNRGIISSKRSKSA"
     gene            1026..2321
                     /locus_tag="VFJ67_04845"
     CDS             1026..2321
                     /locus_tag="VFJ67_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HET7289748.1"
                     /translation="MADRKFGFETLCIHAGQIPDSETGSRAVPIYQTAAYVFDSADNA
                     ASLFNLQTFGNIYTRIMNPTNAVFEERMAALEGGRAAVGVSSGMAAQMVALLTILKSG
                     DEIVSSSTLYGGTYTQFDVNFRDLGINVNFVNPDEPENFRKAITKKTKALFAETIGNP
                     FGNVLDIEAVSAIAREAGIPLIIDNTFATPYLCRPIEFGADIIVHSATKFICGHGTSI
                     GGVLVESGAFPWDNGNFPGMTEPSKAYHGVRFYETFGDFGYTMKARCEILRAYGPALS
                     PFNAFLFLQGLETLNLRMERHCSNALAVAEFLKGHPAVTWVNYPGLPDNPYYGLARKY
                     LPRGSGSIFTFGIKGGKEAGIRFIESVEFLSHLANVGDAKTLVIHPASTTHRQLTEDE
                     QEEAGVNPEMVRISVGLETLDDILWDIDQALTSSQKAKA"
     gene            2367..3269
                     /locus_tag="VFJ67_04850"
     CDS             2367..3269
                     /locus_tag="VFJ67_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7289749.1"
                     /translation="MDKTLKVGIALGGGGAKGFAHIGVLDALGRAGIEFDMVAGTSIG
                     ALVGAVYASGNLPELERISRKYGVKDLPFLLGPTWPRKGLFSGNYIERLLGDIVRVQN
                     IEDLGKPFAALAVDLNKAEVVTFTSGNLYRAVHASMSIPGLFKPVIDGDRILVDGGVL
                     EPVPLSALRRLGADVVVAVDLLSNLTPVGTAPEKTPVFAEYIRFLAERFYMEGLIEAK
                     EREREIAVSLVDIIQRSSIIAQRRLTEYNFRDNPPDVVIDPPLSDIKVLDFHRGESIV
                     DTGRRAAEKAIPELRNILKRAAAR"
BASE COUNT          765 a          898 c          993 g          701 t
ORIGIN      
        1 tttatcctcc tgtaatggaa cacgggctgt atgatccttc tctgcctcag atgcaggtcg
       61 ccctcgctcg tgaggagccc ttctccgagg actagttttg cgcggtttag agcgcggctc
      121 ttggtgaagt tgcggttgtg cgtgacgagc acgtccttga tgtgctccgg gttattgagg
      181 agatatatgc gccgcgatcc cagagtaaaa taaactatgt ccccgtactc gtgcgctatc
      241 ctcgtgagaa agccgagagg gtcgctgaaa tatccggcga gcgagccaat atagggcagt
      301 cctcttggcc ccggagggag tatcggcttc ttgagccggt attctttatt cgccgcttcc
      361 attctgaatc aagaatatgg aatcggatat agagtgtcaa caacactctt taccgggact
      421 ccgcgcctcc cgattttctt gtaatcggtc ggttgtctcg tgtaaattat tccgggaggg
      481 gagacgccaa tggaagacgt aaagactctt cgcaggatac tcagggagaa taaaacgatc
      541 gccgtcgtcg ggctctccga caaatggcac cgtccgagct acttcgccgc gaaatacctc
      601 caggagcacg gctacaggat tatccccgtc aacccgggac acaaggagat acttggtgag
      661 aaatgctacg cgagcctcct cgacatcccg gacaaggtgg acgtcgtcga tattttccgt
      721 aaggcggagg atgtccctcc catagtcgaa gacgcgataa agataggtgc gaaggtcgtt
      781 tggatgcagc tcggaataat aaacgaagaa gcggccaaac gggcgcggga agcggggctc
      841 gaggtcgtca tgaacaggtg catgaagata gagcacgggc gtctcttcgg aggcataaat
      901 ttcatagggg ttaaccgcgg gatcatttca tccaaaaggt ctaaatcggc gtaggaagcc
      961 ttgagtcgtc accgttaagt ggtcacggct ttgtatcgaa gcgcataata tcgggagggg
     1021 taaacatggc tgacagaaaa ttcgggttcg aaacgctgtg tattcacgcg ggacagatcc
     1081 ccgactcgga gaccggctcg cgcgccgtgc cgatctacca gacggccgcg tacgtattcg
     1141 acagcgccga caacgcggcg agcctcttca atctccagac gttcggaaat atatacacgc
     1201 gcatcatgaa ccccacgaac gccgtgttcg aggaaaggat ggccgctctc gaaggaggca
     1261 gggccgcagt cggcgtttcc tccggcatgg ccgcccagat ggtggctctc ctcacgattc
     1321 tcaaatcggg cgacgagata gtatcgtcga gcacgctcta cgggggcacg tacacgcagt
     1381 tcgacgtaaa tttcagggac ctcggcatta acgtgaattt cgtcaacccg gacgagcccg
     1441 agaatttcag aaaggccata acgaagaaaa cgaaggcgct cttcgcggaa accattggca
     1501 atccgttcgg gaacgtgctc gacatcgaag ccgtttccgc gatcgcgcgc gaggccggca
     1561 taccgctcat catagacaat acctttgcga ctccttatct ttgcaggcca attgaattcg
     1621 gggccgacat tatcgtgcac tcggcgacaa agtttatctg cggtcacgga acctctatag
     1681 gcggagtgct cgtcgaatca ggcgcgttcc cctgggataa cggcaacttc cccgggatga
     1741 ccgagccctc gaaggcttat cacggagtga ggttctacga gacgttcggg gatttcggat
     1801 acacgatgaa ggcgaggtgc gagatactga gggcctacgg ccccgcgcta agccctttca
     1861 atgcgttcct cttccttcag gggctggaaa ccctcaacct caggatggag aggcactgct
     1921 cgaacgctct agccgtcgcg gaattcctga agggccaccc cgcagtcacg tgggttaatt
     1981 accccgggct gcccgataac ccctattacg gtcttgcgcg gaaatacctg cccaggggct
     2041 cgggctcgat attcacgttc ggaatcaagg gcgggaagga agcggggatc agattcatag
     2101 agagtgtgga gttcctgagc cacctcgcca acgtgggcga cgcgaagacg ctggtgatcc
     2161 atccggcgtc gaccacgcac cgccagctta ccgaggacga gcaggaggag gcgggcgtga
     2221 acccggagat ggtcaggatt tcggtcggtc tcgagaccct ggacgacatc ctctgggaca
     2281 tagaccaggc tcttacgagc tcgcagaaag cgaaggcgtg accgtgcccg tcgcgtaccg
     2341 gatgatttca tgctgcatcg aataatatgg ataagacctt gaaagtcggg atcgcgctcg
     2401 gaggcggagg ggctaaaggg ttcgctcata taggagtcct cgacgctctc ggcagggccg
     2461 ggatcgagtt cgacatggtc gcgggtacga gcataggagc gctcgtcggc gccgtgtacg
     2521 cttcggggaa tctcccggag ctcgagcgta tctctaggaa gtacggtgtg aaggatctgc
     2581 cgtttctgct aggtcccacc tggccgcgta agggtctctt cagcgggaat tatatagagc
     2641 gtctcctcgg cgatatagtc cgtgtgcaaa atatcgagga tttgggtaaa cccttcgccg
     2701 ccctggccgt cgacctcaat aaagccgagg tggttacctt caccagcggg aatctctaca
     2761 gggcggtgca cgccagcatg tccatacccg gactgttcaa gcccgtgatt gacggggaca
     2821 ggatactcgt cgacggcgga gtgctagagc ccgtaccttt aagcgcgctc aggcgtctcg
     2881 gcgcggacgt cgtcgtagct gtcgatctcc ttagcaatct gaccccggtg gggacggctc
     2941 ctgagaaaac gcccgtgttc gccgaatata tccgcttcct tgccgaaagg ttctatatgg
     3001 agggtctgat tgaggcgaaa gagcgggaga gggagatcgc cgtgtcgctt gtcgatataa
     3061 ttcaaaggag ctctatcatc gcccagagaa ggctgacgga gtacaatttc agggacaatc
     3121 cgcccgacgt cgtcatcgac ccgccgctct cggatatcaa ggtgctggat ttccataggg
     3181 gcgagtcgat tgtagatacg ggcaggaggg ctgctgaaaa agcgataccg gagttgagga
     3241 atattctcaa aagagcagcc gcccggtaac ctctctcctt cccaatttcc attcgataaa
     3301 gagcagtacc catacaggta cgaattcgag ccatattata acgttcgtga atacgcc
//
LOCUS       DASSJH010000224         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_916300, whole genome shotgun sequence.
ACCESSION   DASSJH010000224 DASSJH010000000
VERSION     DASSJH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916300"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFJ67_04855"
     CDS             complement(<1..793)
                     /locus_tag="VFJ67_04855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7289750.1"
                     /translation="MRVLLLNDYGILQGGAEIQMSRLRQLLRDRGHDARLFTSDAKID
                     DLTNVADYTCQGTTSHFRTLLQTANPWAYTGLRKVMREFRPDIVHVKIFLTQLSPLIL
                     PLLKDVPSLYHVAWYRPVCPTGTKMLPDGSACTVRAGTPCYLNGCLPIRDWIPIMTQM
                     ALFRRWRSAFGAILANSESVRDILSQNGIDGVEVLHYGIERRPDGHSLNETPTAAYAG
                     RLVREKGVDILLRAFAKMTGKLPDARLIITGDGPDRDRTERLISEL"
     gene            complement(818..1801)
                     /locus_tag="VFJ67_04860"
     CDS             complement(818..1801)
                     /locus_tag="VFJ67_04860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7289751.1"
                     /translation="MIENRPPLMSVIITTVGGYESVRTVIEHLKMQTVADRLEVIIVS
                     PSMRLTAAEEAKLKHFSNSIIMEADLDNGLSDAWVDAVNESRAPIIAFGENHAFPEPQ
                     WAEALIEAHKGPWAAVGSVIRNANPGSINSRAQLYMTYGRWTGPIESGEAEDLPGHNS
                     SYKRSILLEYGNELKYMLVRANIMHMDLRARGCRLYMEERAAVVHINVSKASSILPDL
                     FYNGQLYTAALVHYKRWSVLKKIFHALLEPLIILRHFQGTMNSLDRDGQRKKLVPAAL
                     PIIIAGLTAHLFGKLKGYASGFGNAQKKINSYEFDRFKHITREDIAELSKL"
     gene            complement(1857..2906)
                     /locus_tag="VFJ67_04865"
     CDS             complement(1857..2906)
                     /locus_tag="VFJ67_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7289752.1"
                     /translation="MMQNVSIGLIGCGRIANLVYLNILRRMPGLRLAAIAEPDGVKRE
                     RAGSAAPQAGLYTDYRDLLRHPDLDAVIICLPNDMHAEAAVSAFEAGKHVYLEKPIAI
                     NTAEAEEVLKAWNGSNLVGMTGFNLRFNPAYQSLKKHIESGTIGEITCVRTVFSYWRR
                     EFPEWRKHRTSGGGALLDIGSHHVDLLRYTLDTDITRVSATTGSHNTEGDNAICEFKT
                     SGGIDVQSYFSITSADEDKIEVYGTRGKLSADRFNSSLRVVISESQRPGLLSQIKGDV
                     SSLLNSRNLKDRILHPAKEPSFDLAIGRFISAVKGADTASPDLLDGYKCLRVLEGAEE
                     SALTGSVVFLGEHQV"
     gene            3179..>4218
                     /locus_tag="VFJ67_04870"
     CDS             3179..>4218
                     /locus_tag="VFJ67_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7289753.1"
                     /translation="MIKKPSQSLNILIVDDEANIRKTLTVCLESLGHSVTAVSNPQDS
                     LSEAGRRVFDLAFVDLRLGTESGLDLIPALLASCPWLKIIVVTAYASIDTAVEAIRRG
                     ASDYIPKPFTPEHIEIVTNRIASVRGMERQIAALEEHLNKINPEITFESRHPEMQRAV
                     ELARRVAPTDAALLIKGPSGTGKTVLARAIHDWSRRSDKPFAVISCPTLSPELLESEL
                     FGHVKGAFTGAVSDNPGRIAACEGGTLFLDEIGDLPLNIQPKLLRFLQEKEFERIGDH
                     RTLRADVRIISATNTDLERVVKEGRFREDLYYRLNVIEITLPPLSERPDDVQELAERM
                     LAFFGAQNHKLI"
BASE COUNT          934 a         1119 c         1117 g         1048 t
ORIGIN      
        1 tcagctctga gatcaaacgc tccgttctat ctcggtccgg tccgtccccc gtgattatga
       61 gcctcgcatc gggcagcttt cctgtcattt tcgcgaacgc gcggagaagt atgtcaactc
      121 ccttttcccg tacgagccgc cctgcatatg ccgctgtcgg tgtttcgttt aaggagtggc
      181 cgtcgggtct cctttctatc ccgtagtgaa gcacttcgac cccgtcgata ccgttctgtg
      241 acaaaatgtc ccttaccgat tcgctgttcg ccagaatagc tccgaaggcg ctccgccatc
      301 gtctgaataa tgccatctgc gtcataatcg gaatccagtc cctgataggc aggcatccgt
      361 tcagataaca gggcgtgccg gccctcacgg tgcatgcgct tccgtcgggg agcatcttgg
      421 tcccggtcgg acagaccggg cggtaccacg ctacgtggta aagagagggc acgtctttga
      481 gaagcggcag tataagcggg gagagctggg tcagaaatat cttcacatgc acgatatccg
      541 gcctgaattc tctcatcacc ttcctgagcc ccgtatacgc ccatggattt gcggtctgga
      601 gaagcgtacg gaagtgagag gtagtgccct gacatgtata atcagctacg ttcgttaaat
      661 cgtcaatttt cgcgtcgctc gtgaataacc tggcatcgtg tcctctatcc ctcagtaatt
      721 gacggagcct cgacatttgt atctcggctc cgccctgaag aattccgtag tcgtttaaga
      781 gcaggactct catatccgtg attctatcga ctgcatgtta cagtttcgat aattccgcta
      841 tgtcttccct cgtgatgtgt ttgaatctgt cgaactcgta gctgttgatc tttttctgcg
      901 cgtttccgaa acccgacgcg tatcccttta atttcccgaa caagtgcgca gtgaggccgg
      961 cgattatgat cggcagggcg gccggcacga gtttttttcg ctgtccgtca cggtcgaggc
     1021 tgttcatagt accttggaaa tgtctgagaa taataagagg ctcgagcagt gcatggaaga
     1081 tttttttaag aactgaccat ctcttgtaat gaacgagcgc ggctgtgtaa agctggccgt
     1141 tatagaagag gtcaggcaga atggaagaag ctttagacac gtttatatga accacggcgg
     1201 ctctctcctc catgtaaagc ctgcaacccc tcgcgcggag atccatgtgc atgatattgg
     1261 ccctcaccag catatacttc agctcgttcc cgtattcgag cagtatcgag cgcttgtacg
     1321 agctgttgtg acccggcagg tcttctgcct cgcccgattc gatcggcccc gtccatcgtc
     1381 cgtatgtcat gtagagctgt gcccggctgt tgatacttcc ggggttggcg ttcctgatga
     1441 cgctccccac ggcggcccag ggccccttat gcgcctctat taacgcctcg gcccactgcg
     1501 gctccgggaa cgcatggttc tccccgaacg caattatggg cgctcgcgat tcattgaccg
     1561 catccaccca tgcgtcggaa agtccgttat ccagatcggc ttccatgatt atagagttac
     1621 tgaaatgttt gagctttgcc tcctcggcgg ccgtgagcct catcgaaggg gatacgatta
     1681 tgacttcgag cctgtccgcg actgtctgca ttttaaggtg ctcgattacg gtacggacgg
     1741 attcgtatcc gccgacggtc gttattatca cggacatgag cgggggtcgg ttttctatca
     1801 tgactatatt ctctagtaaa gcattattga caggatgtgt cgaattatca gagacgttac
     1861 acctgatgct cgcccaagaa tactacgctt cccgtcagcg ccgattcttc ggccccttcg
     1921 agaaccctga gacacttata cccgtcgagc agatccgggc tagctgtatc cgcccctttt
     1981 actgccgaga tgaaacggcc gatcgcgaga tcgaacgacg gttctttagc ggggtggagt
     2041 atccggtctt ttaaatttcg ggagttaaga agcgaactga catcgccttt gatctgcgat
     2101 aatagtcccg gccgctggga ttccgagatt acgaccctga ggctggaatt gaatctgtcg
     2161 gctgagagct tgccccgtgt gccgtaaacc tcaattttat cctcatctgc ggaagttatc
     2221 gagaaatagg actgaacatc gatgcctccc gatgttttaa actcgcatat agcgttatcc
     2281 ccctcggtat tatgagagcc ggttgttgcg gaaacacgtg taatgtccgt gtcgagcgtg
     2341 tatcgtagga ggtccacatg gtgagagccg atgtcgagca gcgctccgcc gcctgaggtc
     2401 cgatgcttcc tccattcagg gaattccctg cgccagtacg agaaaactgt cctaacgcat
     2461 gttatttccc cgatagtgcc tgattcgata tgttttttaa gcgactgata ggcgggattg
     2521 aaacggaggt tgaatcccgt catgccgacg agattactgc cgttccatgc cttcaggact
     2581 tcttcggcct cggccgtatt tatagcgatc ggcttttcga gataaacgtg ctttcccgcc
     2641 tcgaacgccg agacggccgc ttccgcgtgc atatcgttcg gaagacagat tattaccgcg
     2701 tcgaggtccg ggtgacggag gaggtctcta taatccgtgt aaagcccggc ctggggagct
     2761 gcgcttccgg ccctctctct ctttacgccg tcaggctctg cgatagcggc cagcctgagt
     2821 ccgggcattc ttcgcagtat attaaggtag accaagtttg ctatacgtcc gcaaccgatg
     2881 aggccgatgc tcacgttctg catcatctgt ttgagctata ctcctgaaaa gaagttataa
     2941 atgtcatttc gatgaagtcc ggtttcattc taacctatac tcggtttatt taacagacgg
     3001 cactgcgaat gcggcatcgg gacgggagat gtggaatagg tataattgat aatataatct
     3061 taaatgtgtg aaatagacgg cttcgctcag tgccgatgtt attaatataa acggccggcc
     3121 atctacaagc cgaaatataa tgatgtaaga atattgtaaa aagagttgca ggtaattgtt
     3181 gattaaaaaa ccttcacagt ctctgaatat cctcatagtc gacgacgaag cgaacataag
     3241 gaagacgctg accgtttgcc tcgaatccct gggtcatagt gttacagcgg tgagtaatcc
     3301 gcaggactct ctttccgagg cgggcaggcg cgtattcgat ctcgctttcg tcgatctaag
     3361 actcggtacc gagagcggcc tcgaccttat acccgcgctt ctggcctcat gcccgtggct
     3421 caagatcata gtcgttacgg cctatgcgtc gatagacacg gctgtcgagg cgatcaggcg
     3481 gggagcatcc gattatattc cgaagccttt tacccctgag catatagaga tcgtcacaaa
     3541 ccgcatagcg tccgtacgcg gaatggagcg gcaaatagcc gccctcgagg agcatctcaa
     3601 caagattaat cctgaaatca ccttcgaatc cagacatccg gaaatgcaga gggctgtaga
     3661 gcttgcccgc agagtggccc cgactgacgc cgccctgctc attaaagggc cgagcggcac
     3721 cggcaagacg gtgctcgccc gcgccataca tgactggagc agaaggagtg ataagccgtt
     3781 cgcagtcata tcgtgtccca cactcagccc ggagctcctc gagagtgagc tcttcggaca
     3841 tgtgaaggga gcgttcacgg gcgccgtatc cgataacccg ggacgaatag ccgcttgcga
     3901 aggagggacg ctgtttctag acgagatcgg ggacctgccg cttaatatac agcctaagct
     3961 gctgaggttt ctccaggaga aggaattcga gcggatcggt gaccacagga ctctcagggc
     4021 ggatgtgcgc ataatttcgg ctacgaatac cgatctggag agagtagtga aggagggacg
     4081 attccgtgaa gacttgtact acaggcttaa cgtcatcgag ataacactac cgcctctctc
     4141 ggagcggcct gatgacgttc aagagctggc ggagaggatg ctcgcattct tcggcgctca
     4201 aaaccacaag ctgattca
//
LOCUS       DASSJH010000225         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_55781, whole genome shotgun sequence.
ACCESSION   DASSJH010000225 DASSJH010000000
VERSION     DASSJH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55781"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /gene="glnS"
                     /locus_tag="VFJ67_04875"
     CDS             complement(<1..930)
                     /gene="glnS"
                     /locus_tag="VFJ67_04875"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HET7289754.1"
                     /translation="MDDDKEKKSLDFIRTIVEEDLESGKFGGRVATRFPPEPNGYLHI
                     GHAKSICLNFGVAEEYRGTCNLRMDDTNPEKEDMEYVDSIIKDVRWLGFDWDDRLFYA
                     SDYYRQLYDYAVELIRKGKAYVDSLSAEEIREHRGTLNKPGRESPYRDRPIEESLDLF
                     ERMRKGEFEEGTHVLRAKIDMSSGNINMRDPVMYRILHEPHYRTGDDWCIYPMYDYAH
                     RISDSIEGITHSLCTLEFEDHRPLYDWFLDELGIYHPQQIEFSRLNLNYTVMSKRRLL
                     ELVQKGYVNGWDDPRLPTISGLRRRGYTPASIRN"
     gene            1051..2034
                     /gene="hemB"
                     /locus_tag="VFJ67_04880"
     CDS             1051..2034
                     /gene="hemB"
                     /locus_tag="VFJ67_04880"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET7289755.1"
                     /translation="MYFPAYRPRRLRRNERLRDLIAETDISAGELIYPMFITPGKGER
                     EEVVSMPGIYKQSVDNIVKEALEAGKLGVNSILLFGIPEKKDETGSEGYDPNGVIQKA
                     VRELKKKAKGLVVITDVCMCEYTSHGHCGIIRDGEVNNDETLEYLARMALSHAEAGAD
                     IVAPSDMMDGRVGVIRETLDENGFGSLPIMAYSAKYASSFYGPFRDAAESPPQFGDRK
                     GYQMDHRNVREAMREIALDIEEGADIVMIKPALSYLDVISAARREFDHPIAAYNVSGE
                     YSMVKAAGRLGWIDEKQVMMEILTSIKRAGADLIITYHALEAARILNSARK"
     gene            2038..2241
                     /locus_tag="VFJ67_04885"
     CDS             2038..2241
                     /locus_tag="VFJ67_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HET7289756.1"
                     /translation="MEYTSYKVVEVPLVTDEELEKVINRWVGEGWVLDSIHFAMRESS
                     RRPSMAFVVFASNRNESAKNHSS"
BASE COUNT          537 a          616 c          673 g          530 t
ORIGIN      
        1 attccttatc gaagccgggg tgtatcccct tctcctcagt cccgagattg tgggcaacct
       61 ggggtcgtcc catccgttga cgtatccctt ctgtacgagc tcgagaagcc ttctcttgct
      121 catgaccgtg tagttgaggt tgaggcgtga gaactctatc tgctgagggt gatatatgcc
      181 gagctcatcc agaaaccagt cgtagagcgg cctgtgatcc tcgaattcga gcgtgcagag
      241 cgagtgcgtt atcccttcta tggagtcgga aatgcggtgc gcgtagtcgt acatcggata
      301 gatgcaccag tcatcacccg tcctgtaatg aggctcgtgg agtatcctgt acatgaccgg
      361 gtcgcgcatg tttatgttgc ccgaggacat gtctatcttc gcccggagca cgtgcgtccc
      421 ctcttcgaac tctcccttcc gcatgcgctc gaataggtca aggctctcct cgatgggtct
      481 gtcgcggtaa gggctttccc tcccgggctt attgagtgtt cccctgtgct cccttatctc
      541 ctcggcgctc aggctgtcga cgtaggcctt gcctttcctg atgagctcta cggcgtaatc
      601 gtagagctgc cggtaatagt ctgatgcgta aaagagcctg tcgtcccaat cgaatccgag
      661 ccacctgacg tctttgatta tcgaatcgac gtactccatg tcttcctttt cggggttcgt
      721 atcgtccatc ctgaggttgc acgtgccccg gtattcctcg gccacgccga agttgaggca
      781 tatggatttc gcgtgcccta tgtggaggta gccgttgggc tccgggggga aacgcgtcgc
      841 gaccctgccg ccgaatttcc cgctttccag gtcctcctcg actattgtcc ttatgaagtc
      901 gaggctcttt ttctctttat cgtcgtccat tgtcgtctgt ccagtcggga attaataata
      961 tacaaacccc cggaaacatc aaaagtttaa atggaataat atatgccgtt tattgactaa
     1021 acgtccaaag agcaattaat attaatagcc atgtatttcc ccgcctaccg tcctagacgg
     1081 ttaagaagaa acgagcgtct gagggatctg atcgccgaga ccgatatctc ggccggggag
     1141 ctcatatacc cgatgttcat cacgccgggc aagggcgaga gagaggaagt agtctcgatg
     1201 ccaggaatat ataagcagtc cgtggacaat atagttaagg aagcgctcga ggcagggaag
     1261 ctcggtgtaa attcgatact cctcttcggc ataccggaaa agaaggacga gacggggtcg
     1321 gaagggtacg acccgaacgg cgttatacag aaggccgtga gggagctcaa gaagaaggca
     1381 aagggactcg tcgtaatcac ggacgtctgc atgtgtgaat acacgagcca cgggcactgc
     1441 gggataataa gggacggcga ggtgaataac gacgagacgc tcgaatacct ggcccgcatg
     1501 gcgttatctc acgctgaggc cggagcggat atcgtggctc cctccgatat gatggacggc
     1561 cgcgttggtg tgattaggga aacgctcgac gagaacgggt tcggcagcct gcctataatg
     1621 gcctattcgg cgaaatacgc gtcctcgttc tatgggcctt tcagggacgc cgccgagtcc
     1681 cctccccagt tcggggacag gaagggctac cagatggacc acaggaatgt gagggaggcg
     1741 atgagagaaa tcgccctcga tatagaggaa ggggccgaca tcgtgatgat aaaacccgcc
     1801 ctctcctacc tcgacgtcat atcggccgcg aggcgggagt tcgatcaccc gatcgccgca
     1861 tacaacgtga gcggggaata ttctatggtg aaggccgcgg gacgcctcgg ctggatcgat
     1921 gaaaagcagg tgatgatgga gatactgacg agcatcaaga gggccggggc cgatctcata
     1981 atcacgtacc acgcgcttga agcggctaga atcctcaaca gcgcccggaa atagactatg
     2041 gaatacacga gttacaaggt cgtcgaagtg ccgctcgtca cggacgagga gttagagaag
     2101 gtcataaacc gctgggtcgg cgagggatgg gtgcttgaca gcattcactt cgccatgagg
     2161 gaatcctcga gaaggccgtc catggccttc gtcgttttcg cctcgaacag gaacgaaagc
     2221 gccaagaacc acagctcatg accggaaacg ttgacctcgc gtctgataag ttccgcacgt
     2281 aatgcagtga taaataaact ctcctgattg aaataacgga tgccggatta gcgttttttg
     2341 ccgaggtccc gctcga
//
LOCUS       DASSJH010000226         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1353773, whole genome shotgun sequence.
ACCESSION   DASSJH010000226 DASSJH010000000
VERSION     DASSJH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353773"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..708
                     /locus_tag="VFJ67_04890"
     CDS             64..708
                     /locus_tag="VFJ67_04890"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HET7289757.1"
                     /translation="MMSKNLLKSAVLSIFAIFIVAGAGAAISLAQNDTEPAKQGVMDK
                     MHQEMMAKWQQYATPGEGHKALEPLVGNWDYTVTWWETPESQPMKSTGSSEVKWIMGG
                     RYLEQTATGTSMGQEFHGMGLMGYDNMKKEYTGVWIDNMGTGMMTSTGTYDPATKTFT
                     EKGTFSCPQDEGGEKSFRGVTTMTSADTYKYEMFVTGKDGKEARMMEIVYTRKK"
     gene            complement(722..1600)
                     /locus_tag="VFJ67_04895"
     CDS             complement(722..1600)
                     /locus_tag="VFJ67_04895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET7289758.1"
                     /translation="MIKNAAKEILSYILWPLLLGVPIYAVYRGIEAGRPTLYFNAAYL
                     SLAASLFVLERVMPYREEWVQSDGQEFPDFAHTVLNKGLIQLALFLLLISGIIETMGH
                     KSAYSFWPSGLPMAVQVLIAMVVSEFGLYWKHRLAHTWDFMWRFHSVHHSVEKLWFFN
                     TGRFHFVDTFGGLLFSIPLLFIMGVPGDVFIYFSSITAFIGLLTHCNIYMHGGILNYI
                     FITPNVHRWHHSKKVEEGNNNYGENLVIFDILFGTYYHPRDRHVGTLGIKEEMPKAFL
                     GQLAAPFVWNKYRKKG"
     gene            complement(1791..2345)
                     /locus_tag="VFJ67_04900"
     CDS             complement(1791..2345)
                     /locus_tag="VFJ67_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7289759.1"
                     /translation="MALTPSNMLPLGTKAPDFKLPNAVTEKELGLDELKSDKATVVMF
                     ICNHCPYVKHVQKGLVELANDYIPKGVSFIAINSNDVAKYPEDSPANMKSVALRLGYP
                     FPYLFDETQEVARAYEAACTPDFYVFDADTKLAYRGQMDDSRPGNGKPVTGRNIRETL
                     DRILSGKPVAADQIPSIGCNIKWK"
BASE COUNT          706 a          688 c          688 g          650 t
ORIGIN      
        1 tcttccatcg tccaaatcgg ttagtatttt aggtgtaatt ccacatacag gaggctatac
       61 agaatgatga gcaagaatct tttgaagtcg gccgtactat cgatctttgc cattttcatt
      121 gtcgcgggcg caggggccgc aatatccctc gcacagaatg acactgagcc cgcaaagcag
      181 ggcgtgatgg ataagatgca ccaggagatg atggccaagt ggcagcagta cgcgacgccc
      241 ggagaaggcc ataaagccct cgagcccctc gtcggcaact gggattatac ggtgacgtgg
      301 tgggaaacac ccgagtctca gcctatgaaa tcgacgggct caagcgaagt gaaatggata
      361 atggggggac gttacttgga gcagacggcg acaggcacca gcatgggaca ggaatttcac
      421 ggcatgggat taatgggata cgacaacatg aagaaggaat atacgggcgt ctggatcgac
      481 aatatgggta caggcatgat gactagcacc ggtacatacg acccggccac caagacattt
      541 accgagaagg gtactttctc atgcccgcag gatgaagggg gagaaaaatc cttccgcggc
      601 gtaacgacca tgacgagcgc cgacacgtac aagtacgaga tgtttgtcac cggcaaagac
      661 ggcaaggaag cccgcatgat ggaaatcgta tacacgagaa agaaataata tccggcctgt
      721 cctagccttt tttccggtat ttattccaga cgaaaggcgc cgcaagctgt ccgaggaacg
      781 ctttgggcat ttcttcttta atcccgagcg ttcctacgtg acggtccctg ggatgataat
      841 aggtcccgaa gagtatatcg aaaataacga ggttctctcc gtagttgttg ttcccctctt
      901 cgactttttt ggaatggtgc cacctgtgaa cgttgggtgt gataaagata tagttcagaa
      961 ttcccccgtg catataaatg ttacagtgcg tgagcagccc aatgaacgcc gttatcgagc
     1021 tgaaatatat aaacacatcc cctgggacgc ccatgataaa aaggagcggt atgctgaaaa
     1081 ggaggccgcc gaaggtatcg acgaaatgga acctccccgt gttgaagaac catagcttct
     1141 cgacgctgtg gtgcacggaa tggaagcgcc acatgaaatc ccacgtatgg gccagacggt
     1201 gcttccagta gagtccgaat tcgctcacca ccattgctat aagcacctgt acggccatag
     1261 gcagccctga gggccagaaa gaataggcgg acttatggcc catcgtctct attatcccgc
     1321 tgatcagcag taaaaagagc gccagttgaa tcagtccctt attgagtacc gtatgggcga
     1381 aatcagggaa ctcctgtccg tctgactgga cccactcttc cctgtacggc ataaccctct
     1441 cgagcacgaa gagcgatgcg gcgaggctca gatatgcggc gttaaaatat aacgtcggcc
     1501 tcccggcttc tattccccga tagaccgcgt atatcgggac gcccagcagg agcggccaca
     1561 ggatgtatga caatatctct ttagctgcgt tctttatcat cgggattacc tcttctcacc
     1621 ctgtgcgact attatagaat tctaaatcaa aattggaatt tgataaggaa aaattatcgg
     1681 aatccctcat tagatataat catacaccgg atattttaag atagtttgaa tttcaccgga
     1741 gcacgtagaa cgtgctgcgg gaatacaatc cgtaagactc aggtctccga ttacttccac
     1801 ttgatattac agccgatgct cggaatctga tccgcggcga ccggcttgcc gctcaatatc
     1861 ctgtcgagcg tctcccttat attcctgcct gtcaccggct tcccgttacc gggcctcgaa
     1921 tcgtccatct gccccctgta cgcaagtttc gtgtccgcgt cgaacacgta aaaatcgggc
     1981 gtgcatgcgg cttcgtaggc cctcgccact tcctgcgttt catcgaagag gtacgggaac
     2041 gggtagccga gcctgagagc cacgctcttc atgttggcag gggagtcttc cgggtatttc
     2101 gcgacgtcgt ttgaatttat cgcgataaac gaaacgcctt tcggaatgta atcgtttgcg
     2161 agctcgacga gacccttttg aacatgtttt acatacggac aatggttgca aataaacatt
     2221 acgaccgtcg ctttgtccga cttcagctcg tcgaggccga gctccttttc agtgactgcg
     2281 ttggggagct tgaaatccgg ggccttagtg cctaaaggca gcatattgga aggtgtaagg
     2341 gccatggtcc gttctcctta aatatcagtt cttttcgtcg ctaaacttta tcgtcctgtg
     2401 cgatccggcg tcgagcacat ctattcggct gaattttcat atcaacatcc gcacaacccc
     2461 gcacagagaa tttaaataaa ccgcatgcct taagcggtac ggatttttat tctacatagt
     2521 atggccgcat aatcctagct tgccggaccc cagcggctgg caggggatca cgaacattcc
     2581 atcataattt accttgagga cggtcgccgt aagaccctgt gattatatgc taaataagca
     2641 tatttggtcc gcacggacat ttgccccgat ctaggagaag caaccaactg ttaacggaca
     2701 cttttggctt caatcaaaat aatacttgac ag
//
LOCUS       DASSJH010000227         5477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_916487, whole genome shotgun sequence.
ACCESSION   DASSJH010000227 DASSJH010000000
VERSION     DASSJH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916487"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VFJ67_04905"
     CDS             complement(<1..1376)
                     /locus_tag="VFJ67_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET7289760.1"
                     /translation="MRQKDSSTRQIIIALLSVSLSIAILDASIGIIGTRAQFLMFRSI
                     MLPIAVTSGFSFVLILVLWKLIGERVVNKYGLERVPAIISIGVFVGVTVLLISINYSI
                     FAQNWPAYRTELIVFLIISSFALVIAYYTAKRINGSQYGALAGSILSIMIPLILTEIM
                     ILIWLDGHGELKEYDLSLGFFSITLLILGIFFVLIKTKFDTIIPLAVLMFSALVVCPV
                     VFVMTKETYEPAPSSFKPSSGHKVRHVILITIDTLRSDALSSYGSTRISTKNIDSLAA
                     DGALFKNAFSAAPWTLPSFSSMMTGLSPTVHKTYTAQSALPNKFKTLAEYMRDSGYYT
                     AAIGDNYFLHPEFNMDQGFIEYNFFPKRQMVVDSFAANVINTAFPGRLNYYASTRDLT
                     DLAAGWIEQNRDSDFFLWVHYYDPHIPYAPPREYISKDAVPDGSIGYALMEAGKIRSG
                     HFAPNASQ"
     gene            complement(1466..2896)
                     /locus_tag="VFJ67_04910"
     CDS             complement(1466..2896)
                     /locus_tag="VFJ67_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7289761.1"
                     /translation="MKLLIIGLDCADPRFVFGWKEELPNLRRLMEKGAYGPMLSCHPP
                     ITVPAWAVMMSGKDPGQLGYYGFRNRMDYSYDGYTIANATAVKCDRVWDILSRRGKKV
                     VLLGVPQTYPPKPVNGCVVSGFLAPSTESNYTYPAPLKDEIEEVAGGYVLDVENFRTD
                     DKEALLGRIYDKTQKHFKVAKHLIRTKPWDFFMLVEMGVDRIHHGFWSYVDPEHRKFE
                     PGNPFENSIKEYYKYCDREIGELLSLVPEDTVVMVISDHGARKMEGGICFNEWLIREG
                     YLTLKSYPQTQTRIADAEIDWSRTIAWGDGGYYGRLFMNVRGREPEGIIEPGDYEKVR
                     SELIRKIEAIPGPDGEDIGTKAHRPEDIYREANGVPPDLIVYFGALGWRSVGSVGRGS
                     IHTFENDTGSDEANHDWNGVFILNENGCRAGNLEPGYRNGIRIYDIAPTILDLFGTKP
                     EGESTGKSLTSAGNGGLFGRLKRILV"
     gene            complement(2897..4891)
                     /locus_tag="VFJ67_04915"
     CDS             complement(2897..4891)
                     /locus_tag="VFJ67_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7289762.1"
                     /translation="MRKTLITVLIVCAMSLISDLVPETSARTGIDGGELIRQKNITGA
                     TTTTAKISPNLLRLLGKMEDKGVTGKGIGSLHADDLSTPLVKVDDQGNVQVYVHMNES
                     NSENIGQLEGLGLIPEVVNDELKIVQGWLPHENVEKAAELGFVSKITPPSYGRPRAGS
                     VTSEGDAIMGSDEARDTFGVDGSGIKVGVISDGVDSLAASQMSGDLPDDVQVGNPGNG
                     DEGTAILEIVHDIAPGAELAFHNGTTTLTFINAINFFRNNGVDVIIDDLGFLSEPFFQ
                     DGSVAQAAADAAADGIVYVSAAGNDQDAHYQALFLDTDPEDGDDEVNLHDFGLAADES
                     SDVGMTIQLPANRTSVVVLQWSNPFGEASDDYDLFLVDSDTGDVLDSSTNIQNGDDDP
                     IEIASIGNDGPGTGFFDIVINKFSGEDQTLEIFFNVSGTPVEFNVPENSVYGHPAAPG
                     VISVGATFEGEVDFFSSEGPSSIFFPSVVTVSGEETGRAAALLEDRDTPTIVAPNRVS
                     TTVPGFTSFAGTSAAAPHAAGVAALVLQALGFGAEVQASGIESSIVSSRQAEEVTNII
                     TGTADDIPPAGYDNVSGFGSINALAAVEEAINENPQPSPSPSPTPTSPPGNNGSDGSG
                     GCSVYGPAGTDIAGTAFANALILLIPAFVTAFGFLRRRKQ"
     gene            complement(5253..>5477)
                     /locus_tag="VFJ67_04920"
     CDS             complement(5253..>5477)
                     /locus_tag="VFJ67_04920"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HET7289763.1"
                     /translation="IRRLKLAAGQSADIHTVYVHLPELRITTDQQRYTCLEAGRLYRF
                     ESLAGDFVRDIEVDAWGLVINYPGLFRRII"
BASE COUNT         1140 a         1520 c         1457 g         1360 t
ORIGIN      
        1 ctctgcgagg cgttgggtgc gaaatgtccc gacctgatct tccctgcttc cataagagcg
       61 tatccgattg aaccgtcggg gaccgcgtcc ttcgatatat actccctggg aggggcatac
      121 gggatatggg ggtcgtaata atgcacccag aggaagaaat cggaatccct gttttgttcg
      181 atccacccgg cagcgaggtc tgtgagatct ctggtcgacg cgtagtaatt caaacggccg
      241 gggaacgccg tatttatcac atttgcggcg aaggagtcta cgaccatctg ccgtttcgga
      301 aagaaattat attctatgaa cccctggtcc atattgaatt cgggatgtag gaaataattg
      361 tcgcctatcg ccgccgtata atacccggaa tcacgcatat attcggccag ggttttaaat
      421 ttattcggca gtgccgactg ggcggtgtat gtcttgtgaa ccgtgggcga aagtcctgtc
      481 atcatggaag agaacgacgg cagtgtccac ggggccgcgg agaacgcgtt tttaaaaagc
      541 gctccatcag cggcgagtga atctatattc tttgtcgata tccgcgtgct cccgtagctc
      601 gagagcgcgt ctgacctgag agtgtctatc gttatgagaa taacgtgcct gaccttgtgg
      661 ccggacgacg gcttgaagct gctcggagca ggctcgtagg tttctttcgt catgacgaat
      721 acgacggggc acactacgag ggccgagaac atcaaaactg cgagcggaat tatcgtatcg
      781 aacttcgttt tgatcaggac aaaaaatatt cctagtatga gcagggttat cgagaaaaac
      841 cccagcgaca agtcgtattc cttcaactcg ccgtggccgt cgagccagat gagtatcatg
      901 atttcggtga ggatcagcgg aatcatgata ctcagtatgg aacccgcgag cgccccgtat
      961 tgagagccgt ttatcctttt cgccgtgtaa taagctatga cgagcgcgaa ggacgaaata
     1021 atcagaaaca cgattaattc agtcctgtaa gctggccagt tctgagcgaa aatactatag
     1081 ttgatcgaga tcaggagaac cgtgactcct acgaatactc cgatcgatat aatggcaggg
     1141 accctctcca ggccgtattt atttaccacc ctttcgccta tgagcttcca taagacgaga
     1201 atcagaacga acgaaaaacc cgatgttacc gctatgggga gcattatcga ccggaacatc
     1261 aagaattgag cccgagtccc gattatcccg atcgaggcgt cgagaatcgc tatagagagc
     1321 gagacggaga ggagggctat tattatctgt cttgtcgagc tgtctttttg ccgcattacg
     1381 ctatattctt cccatcccgt cacacgttcg tcaataccgg aggagatcgt ccgcttcccg
     1441 gatacgtatg caggcttgaa tcgtattaaa ccaagatcct ttttagccgg ccgaagagac
     1501 ccccgtttcc ggcggatgtc aggcttttcc ccgtagattc accctctggc ttcgtaccga
     1561 aaaggtcgag tatggtagga gcaatatcat agatcctgat accgttcctg tagccgggct
     1621 cgaggttccc cgccctgcac ccgttctcgt tcaatatgaa tacgccgttc cagtcgtggt
     1681 tcgcttcgtc ggaccccgtg tcgttttcga acgtgtgtat gcttcccctt ccgacgctcc
     1741 cgacagaacg ccacccgagc gcaccgaaat agacgatgag atcgggcggg actccgttcg
     1801 cttccctgta tatgtcctcc ggcctgtgcg ctttcgttcc gatgtcttct ccatcagggc
     1861 cgggtatagc ctctatcttt cggatgagct ccgagcggac cttttcataa tcccccggct
     1921 ctattattcc ctcgggctct ctgcccctga cattcataaa gaggcggccg taatagcctc
     1981 cgtcacccca ggcgatggtc ctgctccagt cgatttccgc gtcggctatc ctcgtctgcg
     2041 tctgcggata gcttttcagc gtgaggtaac cctcccggat gagccattcg ttgaagcata
     2101 ttcccccttc catcttcctc gccccgtggt cggatattac catcacgact gtatcctcag
     2161 gtacgagcga caggagctcg ccgatctccc tgtcgcagta tttgtaatat tcctttatgg
     2221 aattctcgaa cgggttgccc ggctcgaatt tcctgtgctc cgggtcaacg tagctccaga
     2281 acccgtgatg tatcctatcc actcccatct cgaccagcat gaagaagtcc cagggctttg
     2341 ttcttatgag gtgctttgcg accttgaaat gcttttgggt tttgtcgtat atccttccga
     2401 ggagagcttc cttgtcgtcg gtcctgaagt tctcgacgtc gagcacgtat ccgcccgcca
     2461 cttcctctat ctcgtcctta agaggtgccg ggtatgtata attgctctcg gtcgaaggag
     2521 cgaggaaccc gctcacgaca caaccgttca cgggtttcgg ggggtaggtc tgggggacgc
     2581 cgaggagcac cacctttttt cccctgcggg ataaaatatc ccagaccctg tcacacttca
     2641 cggccgtcgc gttcgctatc gtatagccgt cgtaggagta atccattctg ttcctgaagc
     2701 cgtagtatcc gagctgtccc gggtccttcc cgctcatcat cactgcccag gcgggtacgg
     2761 tgatcggggg gtggcaggaa agcatggggc cgtaagcccc cttttccatg agcctcctga
     2821 ggttggggag ctcttccttc cagccgaata cgaatctcgg gtcggcgcaa tcaagcccga
     2881 taatcaaaag cttcattcac tgcttccttc ttctgaggaa accgaaagcc gtgacgaagg
     2941 ccggtatcag aaggatcagt gcatttgcaa aggcggttcc cgcgatatcg gtgcctgcgg
     3001 gaccgtaaac agagcatccc ccgctgccgt cagagccgtt gtttccggga ggggatgtgg
     3061 gcgtaggact gggtgacggt gaaggctgcg ggttctcatt gatggcttcc tcgaccgcgg
     3121 cgagggcatt tatgctcccg aagcccgaaa cgttatcata cccagcgggc ggaatgtcgt
     3181 ccgcagtgcc cgtgataata ttcgtgacct cctccgcctg tctcgacgag acgatagagg
     3241 attcgatacc gctcgcttgt acctccgcac cgaatccgag cgcctggagc acgagcgccg
     3301 ccacaccggc tgcgtgaggc gccgcggccg aagttcccgc aaagctcgtg aagcctggaa
     3361 cggtggtcga taccctgttg ggggccacta ttgtaggcgt atccctgtcc tcgagcagcg
     3421 cggctgcgcg cccggtctct tcgcccgaga ccgtcactac cgacgggaag aatatggacg
     3481 aaggcccttc ggagctgaag aagtcaactt ctccttcgaa tgtcgcgccg acggatataa
     3541 cgcccggcgc ggcagggtgt ccgtagacac tgttctcggg gacgttgaat tccactggcg
     3601 ttccgctcac gttgaaaaat atctcgagcg tttggtcttc accgctgaat ttatttataa
     3661 ctatgtcgaa aaagcccgtt cccggaccgt cgttgccgat cgatgcgatt tctatcgggt
     3721 cgtcgtcgcc gttctggata ttagtgctgg aatcaaggac gtcccctgta tcggaatcga
     3781 caagaaaaag gtcgtagtcg tccgaggctt ctccgaaagg gttactccac tggagcacga
     3841 cgactgaagt ccggtttgct gggagctgta ttgtcatccc tacgtcgctt gactcgtccg
     3901 cggccagccc gaagtcgtgc agattgacct cgtcatcgcc gtcctcgggg tcggtatcga
     3961 ggaaaagggc ctgataatgt gcgtcctgat cgttccccgc cgcggatacg taaacgatac
     4021 cgtcagccgc cgcatccgct gccgcctggg cgacggagcc gtcctgaaag aaaggctcgg
     4081 acaggaatcc gaggtcgtct attattacgt ccacgccgtt gttcctgaag aaatttatcg
     4141 cgtttataaa ggttagggtc gtcgtcccgt tgtgaaacgc gagttccgcg ccgggagcta
     4201 tatcgtgaac gatctcaagt atggctgtgc cttcgtctcc gttgcccgga ttgccaacct
     4261 gaacgtcgtc cggaaggtcg cctgacatct gcgaggcggc gaggctgtct acgccgtccg
     4321 atatgacgcc cacctttata cccgacccgt cgacgccgaa ggtatccctg gcttcatccg
     4381 accccattat agcgtcccct tcggacgtaa cagatcccgc tctcgggcgc ccgtagctgg
     4441 gcggggtgat cttcgagacg aaaccgagtt cagccgcttt ttcaacgttt tcgtggggga
     4501 gccagccctg tacgattttc agctcgtcgt ttacgacttc cgggattaaa ccaagccctt
     4561 ccagttgccc gatgttttca ctgttcgatt cgttcatgtg cacgtaaacc tgcacgttcc
     4621 cctgatcgtc caccttcact aagggtgtgg aaagatcatc cgcgtggaga ctcccgatgc
     4681 cttttccggt cacgccctta tcctccattt tgccgagtaa tctgaggagg ttcgggctta
     4741 tctttgcagt tgttgtagta gctcctgtaa tatttttttg tcttattaat tctcctccgt
     4801 ctatcccggt tcgggcgctt gtttcgggga ccagatcgga tatcagagac attgcacaaa
     4861 cgattagcac cgttatcagg gtttttctca ttaagaagct tctcccgggg atgatctcga
     4921 ttttaatatt ataaccaatc ggccgccatt catagcggat aatttaaagc ggccggcatc
     4981 ataatttccc ctgatcacgg cggattgtat gttctttatg acgacaacat tataccgggc
     5041 gccctgcctg ggaggagtca cgatcgtctg cccccgtcac tctgacctca cgggagtgtg
     5101 aatctgttaa ttaactcacc tatatcgctg catttatagg cgacgataat cggcgctgcg
     5161 gcgaaagccc cttcctttat tattatattg ggctgcattt ttgtagtcgt gcagcgccgg
     5221 ctgtacggta tagcatgagg ctgcatgcaa agctatatga ttctcctgaa gagaccgggg
     5281 tagttgatga cgagccccca ggcgtcgacc tcgatatctc tcacgaaatc accggcgagc
     5341 gattcgaacc ggtagagcct gcctgcctca aggcatgtgt agcgctgctg gtcggttgta
     5401 attctaagct ccggtaaatg aacgtacacg gtgtgaatat ccgcggattg cccggcggcg
     5461 agcttcagcc gccgtat
//
LOCUS       DASSJH010000228         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1026200, whole genome shotgun sequence.
ACCESSION   DASSJH010000228 DASSJH010000000
VERSION     DASSJH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026200"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFJ67_04925"
     CDS             complement(<1..858)
                     /locus_tag="VFJ67_04925"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET7289764.1"
                     /translation="MAEEKPLTTEITRYVSNTDRDIYTVSNIPEEVIAVIFAYVSRSP
                     KSFRENIGTVIEEEELGRERASRFHEKWVLNYGHASVAEHAAVHVGIEKVSRLFSSIL
                     ELSNEYLSFTEYSQRYQRPVRGDFFVPGELDHHASLRDEYMKLNDELYTIYSGLNEKL
                     LAHLKKTEPAPEGTDEKAHLRALEKIAFEDARYALTLATFTNLGMTANARAIEDSLTK
                     LLSNKYEEVRRRAEEIKNEVRFSVPTLVKYANENRYIKETGQELTEIARRMLGGGVSK
                     TSNKGPAVTL"
     gene            complement(931..1683)
                     /locus_tag="VFJ67_04930"
     CDS             complement(931..1683)
                     /locus_tag="VFJ67_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HET7289765.1"
                     /translation="MKNDVAGTVAEWIGEARKIVVLTGHELSAESGLPDFTDTRLNPH
                     IRDFRESRDVRAGYWKKIMDIYPALQGAEPGPGHRALAELEMLGNLDCIFTQCADGLH
                     HRAGSTTVIELNSSILWITCPGCGKDYSLDEIAAMLEKGADVPVCRECGSDQLKPAIS
                     FPGQPPPHWEAREAWMRLHNADLFLIAGASLAREPVASYPFLARENNARVAIISEKEG
                     PADDFVDAVIYGKPGQVLSYIIKKVKEGITIS"
     gene            1839..2180
                     /locus_tag="VFJ67_04935"
     CDS             1839..2180
                     /locus_tag="VFJ67_04935"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET7289766.1"
                     /translation="MKCKPAEMTEFSDTALRILWDDGHESIYLYEELRQECPCATCRQ
                     LRKTSRDGRLPFKKTIPLRAKTSAIRPLTIEPVGQYAFKFGWNDKHDTGIYTFEFLRG
                     LCSCEECSPGA"
     gene            complement(2186..2905)
                     /locus_tag="VFJ67_04940"
     CDS             complement(2186..2905)
                     /locus_tag="VFJ67_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET7289767.1"
                     /translation="MRSRSAFLFALLLLIFTVCYSSNAQENQDARRTLSVIGNGKADA
                     APDVAYLTLSVETIAKNASQAVKDNAETTNKVIQVIKAKLGKTDKVSTAGYNLAPVYE
                     YNNQTNKNEFNGYRASNRIIVEAHNLKELGNIIDATAKAGLNNIESLSFDTTKRDEFR
                     REALTRAVNDAKTTAETLAKAAGVKITKILQLSPSFDYPVPVYRDYALAKEAAPAAPP
                     TPIEPGDVSVSVSVNIVFEIE"
BASE COUNT          594 a          816 c          786 g          812 t
ORIGIN      
        1 gagcgtaact gcggggcctt tattcgacgt tttggaaact cccccgccga gcatccgcct
       61 cgcgatctcc gtgagctcct gtcccgtctc cttgatgtat ctgttttcgt tcgcgtattt
      121 tacgagagtc ggcaccgaga acctgacttc gttctttatc tcctcggccc tccgcctgac
      181 ctcttcatat ttattcgaga ggagcttggt gagggagtct tcgatagccc tcgcgttcgc
      241 tgtcattccc aggttcgtga atgtcgcgag agtgagcgcg taacgggcgt cctcgaacgc
      301 gatcttttcg agagccctca aatgagcttt ctcgtccgta ccctccggag cgggctcggt
      361 cttttttaaa tgggcgagga gcttttcgtt cagcccggag tatatggtat aaagctcgtc
      421 gttcagcttc atgtattcgt ccctgagcga tgcatgatga tcaagctcgc cgggcacaaa
      481 gaaatcaccg cggaccggcc tctggtatct ctgcgaatat tcggtgaacg ataggtactc
      541 gttagagagc tccagaatgg acgagaagag cctggatacc ttttctatac caacatgcac
      601 cgcggcgtgc tcggcgacag aggcatgccc gtagttgagc acccattttt cgtggaacct
      661 ggaggccctt tccctgccta actcttcttc ctcgattacg gtaccgatat tctccctgaa
      721 gctctttggg ctcctgctca cgtaggcgaa tattacggct atgacctctt ccgggatatt
      781 gctgaccgtg taaatgtccc tgtccgtgtt ggatacgtat cttgttattt cggtcgtgag
      841 cggtttttcc tcagccattt ctctcctctg aatttccctt tgaaataatg tctggacccg
      901 ctttgggagt aactattata ccgttatcgg tcaggagatg gtaatgcctt ccttcacctt
      961 ttttataatg tagctcagta cctgtccggg cttgccgtat atgactgcat cgacaaagtc
     1021 gtcggccggc ccttccttct cgcttatgat cgcgaccctg gcgttgttct ccctggcgag
     1081 gaacgggtaa gaggcgacgg gctccctggc gagggacgcg cctgctatta gaaaaaggtc
     1141 ggcgttatgg agcctcatcc aggcctcccg ggcttcccag tggggcggag gctgtccggg
     1201 gaatgagatc gcaggtttga gctggtcgct cccgcattcc ctgcacactg gaacgtctgc
     1261 gcctttttcc agcatcgcgg ctatttcgtc gagcgaataa tccttcccgc agcccggaca
     1321 cgtgatccag aggatgctgc tgttgagctc tatcacagtc gtgcttcccg ccctgtggtg
     1381 gaggccgtcc gcacactgtg tgaatatgca gtcgaggttc ccgagcatct caagctcggc
     1441 cagcgcccgg tgtcccggac ccggctcggc accttggagg gccgggtata tgtccatgat
     1501 tttcttccag taaccggccc ttacgtccct gctctcacgg aaatctctta tgtgggggtt
     1561 aagcctggtg tcggtaaaat cgggcagccc gctctctgca gaaagctcgt gccctgtgag
     1621 gactactatc ttccgggcct ctccgatcca ctccgctacc gttccggcga cgtcgttttt
     1681 catctgaaat ggattataca attccgggac aaaatatgtc aacgaaacct tttcgctgct
     1741 ttagcgttcg atgttctgga aatcgggagt caagcgccgc cggattcggt acaatatatc
     1801 tgcacggtac aaacgtatag tccgggagat acttagctgt gaaatgcaag cctgcggaaa
     1861 tgaccgaatt cagtgataca gccctacgga ttctgtggga cgacggccac gaaagcatat
     1921 atctatacga ggaactaagg caggaatgcc cgtgcgctac ttgcagacag ctcaggaaaa
     1981 cgagcagaga cggcaggctc cccttcaaga agactatccc tctcagggca aagacttcgg
     2041 cgatcaggcc tctcactatt gagcccgtgg ggcaatacgc gttcaagttc ggctggaacg
     2101 ataaacacga tacggggatt tatacgttcg aattcctgag agggctctgc agctgcgagg
     2161 agtgcagccc gggcgcctga cccgtttact ctatctcgaa cacgatattg actgacacgc
     2221 tgacggatac gtcaccgggc tctatcggcg tgggcggagc ggccggtgcc gcttccttgg
     2281 ccagggcgta gtctctgtaa acgggtaccg ggtaatcgaa ggatggtgag agctggagta
     2341 ttttagttat cttgacccct gccgccttcg ccagcgtctc ggcggtagtt ttcgcgtcat
     2401 tcaccgcgcg agtcagcgcc tctctcctga attcatctct ttttgtggta tcgaaagaaa
     2461 gactctcgat gttgttgagg ccggctttgg cagtggcgtc gattatattc ccgagctctt
     2521 tcaggttatg agcttctact attatcctgt tgctggccct gtacccgttg aactcgttct
     2581 tgttcgtctg gttattgtat tcatacacgg gggcaagatt atagccggct gtcgagacct
     2641 tatctgtttt gccgagcttt gcttttatca cctgtattac cttgttcgta gtctcggcgt
     2701 tatccttgac ggcctgggaa gcgtttttag ctatggtctc gacggagagg gtaaggtagg
     2761 cgacatccgg ggccgcgtcc gctttcccgt tcccaatgac gctcagcgtg cgcctggcat
     2821 cctgattttc ctgggcattg gaactataac acactgtgaa tattaacaat aataatgcaa
     2881 ataggaatgc cgatctgctc ctcatttctc acctcatttc tatgtttact cgtctcaatg
     2941 acgcatttta ttatactcga tatttacgaa cgaattcgga taaggatttc actcccatat
     3001 agaaacca
//
LOCUS       DASSJH010000229         4280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_917213, whole genome shotgun sequence.
ACCESSION   DASSJH010000229 DASSJH010000000
VERSION     DASSJH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917213"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFJ67_04945"
     CDS             <1..842
                     /locus_tag="VFJ67_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7289768.1"
                     /translation="WALGRTGQATEPQIAEITKLLKDKSPKVRINAVDALLSLDAPAR
                     KHNADIAALLKDPDVRVRTITATALGRMGSSAADQAPALGDLVTAANDDKQVNMDTML
                     AALGALGEIGHAAGGQADKVAPVLGSKKTVLRIAARKSLWMISPLGRNVVMQILGATY
                     RYPAEADQLRLLAHYGSAGGEESETLIRWIGAGADKVPGELNRKEGAQILGIYLDNWG
                     FTEGRKEMRDDFVVRISETVKAGTWTKEDLPLLKKAGETLKTGKYENQANAVNETIGK
                     VQG"
     gene            1008..2252
                     /locus_tag="VFJ67_04950"
     CDS             1008..2252
                     /locus_tag="VFJ67_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7289769.1"
                     /translation="MFGKKIFYGWYIVAASLFIIILDGLLLYSFGVFLPYLNEDFGLS
                     RAAGSSLFSFRSLVQAPAFILAGRMIDRFDPRAVIFGGGLIAAVGMFLSGIAETTWQL
                     YISYGFFIGLGDAVLYITCVALVSRWFVKKRALAIGIITTGVPLSGLITNPLTAWLIK
                     GFGVRNALFALGALMLTVLMSAFVLRSRPEDKNLRPYGEENGGEYEKSREGSGIPEDG
                     SDWGAIEAISTPNYWLMYSMYFLGFTTFLIIVTQFYNFEIDLRVAALAAAGPPAAIGL
                     GSIIGRTLLSSILAEMLEYRKVLLICFFAQGSSILLLLFFDHIWAFYLFGFLFGFFYS
                     AWVPIFPTLLGKFFGLRALGTIYGIFGTSYSIAAIGGPLLAGYVHDVAGTYTYPFVFT
                     IICCYLAAAGAFFIKAPVRKAA"
     gene            2341..3822
                     /locus_tag="VFJ67_04955"
     CDS             2341..3822
                     /locus_tag="VFJ67_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289770.1"
                     /translation="MNADTLRFIRSEGAGLLSGTRRELWLNLTGMRARTRLVGVYGSR
                     RDFLEPELFLSCGGYAGGEEEEKTRLKLLSSFLARAFLSGKTAGLSDRILAFEAGEEF
                     SAGGKRMTLRSAKARILSEPRKYKRDEIDVRAGELLSGLNTLCLRKLESLSECSEALG
                     FKTYGELLDAVHGIASVSLGGEAEKFLRDTDYIAGDLLEWFLTRKIDVRMKDASLSDI
                     TFLFNSAELGGYFPKPGFESFTGTVLGGMSLRPPRQVSFDTVKRMGKTADGFSLTLGP
                     SVETAVSIFPVGGIHDYESLLGCLGHSFCYAYTDPGEDFEFVYLRDPCLTDIFSGLFM
                     DLIYEPVWLKRYLRIDMSGDLHNFLHLKRLMSARIDAGRALCIREIYGGGGPGEVPAL
                     LTDIMSGAAKCRYDGRGFLPEFLSPVRSPFRFKALLAAHGLRTFMKEAFDEEWWRAGT
                     AGDYLSGVWSGGGRITGGSLLERCGCGEEGPEVLVRGFEKAFR"
     gene            complement(3837..>4280)
                     /locus_tag="VFJ67_04960"
     CDS             complement(3837..>4280)
                     /locus_tag="VFJ67_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289771.1"
                     /translation="TSWIKDPERLQDLISESEAIQDKIWSLSVEAVAKTPNQEITSAY
                     IESLNEVIDLHTKRVTVALMYRIPTLIWYALIFVTVLAMGVVGYQFGFTGGRSIFITI
                     ILALTFSAVIYLIADIDRSLGGAIRVSQQPMIELQKKIDEVVPPH"
BASE COUNT          943 a         1181 c         1278 g          878 t
ORIGIN      
        1 cctgggctct cggacggacg ggacaggcga ccgagcctca gatagcggag ataaccaagc
       61 tgcttaaaga caagtccccg aaggtccgga tcaacgccgt cgacgcgctt ctcagcctcg
      121 acgctcccgc gaggaaacac aatgcggaca tagccgccct cctgaaggat ccggacgtga
      181 gggtgcggac gatcacagcg accgcactcg ggagaatggg ctcctcggcc gcggatcagg
      241 ctccggcctt gggcgacctc gttacggcgg cgaacgacga caagcaggta aacatggata
      301 caatgctcgc ggctctgggc gcgctcgggg agatagggca cgccgcgggc ggccaggcgg
      361 ataaggtcgc gccggtgctc ggcagcaaga agacagttct caggatcgcc gcgagaaaat
      421 ccctctggat gatcagcccc ctcggcagaa atgtagtcat gcaaatcctc ggcgccactt
      481 accgctatcc ggccgaggcc gaccagctca ggctgctcgc ccactacggc agcgccggcg
      541 gcgaggagtc ggagacgctg atcagatgga tcggcgcggg ggcggataaa gttccgggcg
      601 agctcaacag gaaggaaggc gctcagatac tcggcatata tctcgacaac tggggcttca
      661 cggagggaag aaaggagatg agggacgatt tcgtcgtccg tatttccgaa accgtcaagg
      721 ccgggacgtg gactaaagaa gacctgccgc tgttaaagaa ggctggtgag acgcttaaaa
      781 cgggcaagta cgagaaccag gcgaatgcgg taaacgaaac gatagggaaa gtgcagggat
      841 aggtcgcccg gccccggcac atcccgccaa cacacactcg ggtccgggaa caaaatgtta
      901 ttttcaggta tgattacccc ccttgacgaa tcggaagtta tttcgcggcc cgtgaaccag
      961 gtaacgccga ctcggactca caagcacgca cgtaaaagag agaccgcatg ttcgggaaaa
     1021 agatattcta cggctggtat atagtcgcgg cttcgctttt tataattatt ctggacggtc
     1081 tcctgctcta ctcgttcggc gtattcctcc cttacctgaa cgaggatttc ggtctcagcc
     1141 gcgcggcggg gtcgtccctt ttctcattca gatccctcgt acaggccccg gctttcatac
     1201 tcgccgggag gatgatagac aggttcgacc cgcgggcggt catattcggc ggcgggctga
     1261 tagctgcggt cggcatgttc ttatcaggca tcgccgagac gacctggcag ctctacatct
     1321 cgtacggatt cttcatcggg ttaggggacg ccgtgctcta catcacctgc gtcgccctcg
     1381 taagcaggtg gttcgttaag aagagggcgc tcgcgatagg catcataact acgggcgtgc
     1441 cgttaagcgg gcttattacc aacccgctga ccgcgtggct gataaagggg ttcggagtga
     1501 ggaacgcgct cttcgccctt ggcgcgctca tgctgaccgt actcatgtcg gctttcgtgc
     1561 tgcgctcgcg gccggaggac aagaatctca ggccctacgg agaggagaac ggaggcgagt
     1621 acgagaagtc acgggaaggg agcggcatcc cggaagacgg gagcgactgg ggagcgatcg
     1681 aagcgatctc gacccccaac tactggctca tgtactcgat gtacttcctc ggattcacga
     1741 ctttccttat tatagtgacc cagttctaca acttcgagat agacctcagg gtcgcggcgc
     1801 tcgctgccgc gggccctccg gcggcgatcg ggctcgggag catcatcggg aggacgctcc
     1861 tttcgtcaat actcgctgaa atgctcgagt acagaaaggt attactcata tgcttcttcg
     1921 cgcaggggag ctctatattg ctgctgctct tcttcgacca catttgggcg ttctacctgt
     1981 tcggattcct gttcggattc ttttacagcg cgtgggtccc gatattcccg acgctcctcg
     2041 gtaaattctt cgggctgagg gcgctcggca caatatacgg catattcggg acttcgtact
     2101 caatagccgc gatcggcgga cctctcctcg caggatacgt gcacgacgtg gcggggacgt
     2161 acacgtaccc gttcgtcttc actataatct gctgctacct tgcagccgcg ggcgcgttct
     2221 ttataaaagc ccccgttcgt aaagcggcct gagcgcgcca ggcatactcg cttctaatga
     2281 ctaggggcgt tctatataat tgataattgc tcacatataa ccgaggaaag gaaattcacg
     2341 ttgaacgccg acacgctaag gtttataaga tcggagggcg ccgggctcct gagcgggaca
     2401 cggagagagc tgtggctgaa cctcaccgga atgagagcgc gaacccgcct agtcggcgtc
     2461 tacggctccc gtcgggattt cctcgagccc gagctctttc tctcgtgcgg gggatacgcg
     2521 ggcggcgaag aggaagagaa aacacgcctc aagctgctgt catcgtttct cgccagggcg
     2581 tttctttccg ggaagacggc gggccttagc gaccgtatac tcgccttcga agcgggagag
     2641 gaattcagcg ccggcggaaa aagaatgacg ctccgctcgg cgaaagcgcg catattatcc
     2701 gagccccgga agtataaaag ggacgagata gacgtaaggg cgggagagct cctctcaggg
     2761 ctcaacacgc tttgcctgag aaagctggaa tcgctctcgg aatgctcgga agccctcgga
     2821 ttcaaaacat acggagagct cctagacgcg gtccacggca tagcgtccgt gagcctcgga
     2881 ggcgaggcgg agaagttcct ccgcgatact gattacatag ccggggactt gctcgagtgg
     2941 ttcctcacga ggaagattga cgtccgcatg aaggacgcct cgctttcgga tataacgttc
     3001 ctattcaact cggccgagct cggaggttac ttcccgaagc ccggtttcga gtctttcacg
     3061 ggaaccgtgc tcggagggat gtcactccgc cctccccgtc aggtcagttt cgatacggta
     3121 aagagaatgg gaaagacggc tgacggattc tcgctaactt tagggccgtc ggtagagaca
     3181 gccgtatcaa tatttccagt gggggggata cacgattacg aatcccttct cggctgtctc
     3241 ggtcattcgt tttgttacgc ctacacggac ccgggagaag acttcgaatt cgtgtaccta
     3301 agagacccct gcctcaccga tatcttctcc gggctcttca tggatctcat atacgagccc
     3361 gtatggctca agaggtattt aaggatcgac atgagcggag acctccataa cttcctccat
     3421 ttaaagagac tcatgtcagc aaggatcgat gcgggacgcg ccctctgcat tcgcgaaatt
     3481 tacggaggag gcggcccggg ggaggtgccc gcgctgctca cggacataat gagcggagcc
     3541 gcgaaatgca gatacgacgg acggggattc cttccggaat tcctatcgcc ggtgcgctcc
     3601 ccattccggt ttaaagccct tctcgccgcg cacgggctaa ggacattcat gaaggaggcg
     3661 ttcgacgagg aatggtggag ggccggaacg gccggggatt atctgagcgg cgtctggtcc
     3721 gggggaggca ggataacagg gggctcgctg ctcgagagat gcggatgcgg tgaggagggc
     3781 ccggaggtgc tcgtccgggg tttcgagaag gctttcaggt aaggatttaa acgagcctaa
     3841 tgcggtggga cgacctcgtc tatctttttc tggagctcta tcataggctg ctggcttacc
     3901 cttatagcac cgccgaggct ccggtcgatg tcagcgatca gatatatcac cgccgagaaa
     3961 gtaagcgcga ggatgatagt tataaagatg ctcctgccgc cagtaaatcc gaactgatag
     4021 cctacgactc ccatagcgag aacggtcacg aatatcagcg cataccaaat gagcgtgggg
     4081 atcctgtaca ttagcgcgac ggtaactctc ttcgtgtgga gatctattac ctcgttgagc
     4141 gactcaatgt atgcagacgt gatctcctga ttgggtgttt tcgcaaccgc ctcgaccgac
     4201 aacgaccaaa tcttatcctg aatagcctcc gactcggata tcaaatcctg aagcctctcc
     4261 ggatctttta tccacgacgt
//
LOCUS       DASSJH010000230         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1463227, whole genome shotgun sequence.
ACCESSION   DASSJH010000230 DASSJH010000000
VERSION     DASSJH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463227"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFJ67_04965"
     CDS             <1..745
                     /locus_tag="VFJ67_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7289772.1"
                     /translation="GVLQAELQKVRHDPVELITRAVQPLLWLVLFGEVMARVRGISPG
                     NLSYIDYLSAGILAQSVLFVAIFFGISAIWDRDLGILHRMLVSPAPRTSIVMGKAFAA
                     SFSGFIQGVIVYSIAGLLGADLSFSPLHIFGVVVLIALGCGLFSTLSLIIACLVKTRE
                     RFMGIGQLLTMPVFFASNAIYPISLMPDWLRTVSSLNPLTYQVDAMRSLMITGVKPEF
                     GLPFDFFILTVITGILIIIAGRLYPRMAV"
     gene            946..2256
                     /locus_tag="VFJ67_04970"
     CDS             946..2256
                     /locus_tag="VFJ67_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289773.1"
                     /translation="MSIPEGLEAALGFGFMDAIFTRRSRRFGLGMEIKEGTLAHKSRH
                     EPVPLSELEEAFLIWAGTGMTGLSLGDLPRTGISWLFQWTGRSWPCSCNSHSTELFYT
                     NDNGVYMVRLHDVMPEDGKSLIFHGMTDDEKLKKVLDLFRKNQIKLDDGRADLPKGEP
                     GLFDFNAWNCNRPGTTFFVPVTNTTVEYMVLLFIYFGAKYGFNIIDELNGGRSCGLDK
                     WIEKGYIRKDVELSLFDLELRVLTTLNVEQAFICQNMNLALQGLGLGGWAFTGLLPHY
                     ALGVHPDYKGLGFRFTKPGKTVRSAKPPVPVGRDGVFEALCPPYVKDMGEAVDKFLRM
                     RGRFWEEDNPYPYQNPAGVLADEYHPSEVRIEIVKDFCKYVYENYGRFPAFLDPMFTR
                     LVFQAHHLDLEFYDKYYTEGAYTELHKNHFHLWHPEMKDPFGRK"
BASE COUNT          499 a          624 c          625 g          511 t
ORIGIN      
        1 cggggtcctt caggcggagc ttcagaaggt caggcacgac ccggtcgagc tgattacgcg
       61 cgcagtgcag ccgcttctat ggcttgtgct cttcggcgag gtgatggcgc gggtgcgggg
      121 aatatccccc gggaacctga gctacatcga ctatctctct gcgggcatac tcgcgcagag
      181 cgtgctgttc gtcgcaatat tcttcggtat atcagcgatc tgggaccgcg acctcggcat
      241 actccacagg atgctcgtga gccctgcccc gcgtactagt atcgtcatgg ggaaggcgtt
      301 tgctgcgagc ttcagtggat tcattcaggg cgtgatagtt tactcgatag cgggattact
      361 cggcgcggac ctcagcttct ccccgctgca tatattcgga gtcgtcgtgc tcatagcgct
      421 cgggtgcggg cttttttcga ccctctcgct cataatcgcg tgcctcgtga agacgcggga
      481 gaggttcatg ggcataggac agctcttgac catgcccgtc ttcttcgcga gtaacgccat
      541 ctatcctata tcgctcatgc ctgattggct gcggacggtc tcttccctga acccgcttac
      601 gtatcaagtt gatgcgatgc gctcgctaat gataacgggc gttaaacctg aattcggcct
      661 cccgttcgat ttctttatcc tcaccgtcat aaccggaatc ctcatcatca tcgccggccg
      721 tctttatcca cgtatggcgg tctaatacgg catcccccct tccggtagat ttcatacgca
      781 cgcaaagcaa taagccatgt ccgataattt aacttatgta tactgtttac ctacaccttc
      841 ctaactggct gcaaactcag tattttatca agataccctc acagatattt caaaatgccc
      901 cataatcgtg taatcctatt caaaaatgcc gtagaggaga cagttatgag cattcccgaa
      961 ggacttgagg ccgcgcttgg attcggattc atggacgcca tttttacgag gcgctcgcgg
     1021 aggttcggcc tcgggatgga gataaaggag ggcacgctcg cccacaagtc gcgccacgag
     1081 ccggtgcccc tatccgagct cgaagaggcg tttcttatat gggcagggac cggcatgacc
     1141 gggctctccc tcggtgacct accgcgtacg ggcatctcat ggctcttcca gtggacgggg
     1201 aggagctggc catgctcctg caattcccat tcgacggagc tcttctatac gaacgacaat
     1261 ggcgtctata tggtgaggct ccacgacgtc atgccggagg atgggaaaag cctcatcttc
     1321 cacggcatga ctgacgacga gaagctcaaa aaagtgctcg atctattcag aaagaatcaa
     1381 atcaagctcg atgacggccg cgccgatctt cccaagggtg agccgggact cttcgacttc
     1441 aacgcctgga actgcaacag gcccgggacc acgttcttcg tccccgtgac gaacacgacg
     1501 gtcgagtaca tggtgctgct gttcatctat ttcggcgcca aatacgggtt caacatcata
     1561 gacgagctta acggcgggag gtcgtgcggc ctcgacaagt ggatagagaa ggggtacatc
     1621 agaaaggatg ttgagctctc gctgtttgac ctcgagctca gggtgctgac gaccctcaac
     1681 gtcgagcagg cgttcatatg ccagaacatg aacctggcgc tccaaggatt agggttaggc
     1741 ggatgggcat tcacggggct cctcccccat tacgccctcg gcgttcaccc cgattacaag
     1801 ggcctcgggt tcaggttcac gaagcccggg aaaaccgtgc gcagcgcaaa gcctcccgtg
     1861 ccagtaggca gggacggcgt tttcgaggcg ctgtgtccgc cttacgtgaa ggacatgggc
     1921 gaagcggtgg acaaattcct ccgcatgaga gggaggtttt gggaggagga taacccctac
     1981 ccttaccaaa accctgcggg agtgttagcg gatgagtacc acccgagcga ggtcaggata
     2041 gagatagtga aggacttctg caaatacgtt tacgagaatt acggaaggtt ccccgcgttc
     2101 ttagatccca tgttcacgag gttagtcttt caggcgcacc atctggactt ggagttctac
     2161 gacaagtact acaccgaagg ggcatacaca gagctacaca agaaccactt ccatctctgg
     2221 catcctgaga tgaaggaccc atttggtagg aagtgaagg
//
LOCUS       DASSJH010000231         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1027059, whole genome shotgun sequence.
ACCESSION   DASSJH010000231 DASSJH010000000
VERSION     DASSJH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027059"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFJ67_04975"
     CDS             <1..559
                     /locus_tag="VFJ67_04975"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HET7289774.1"
                     /translation="RNGTLTSIAPTGTISIIAGTTSGIEPLFALAYERKNVLRGETLY
                     EINPLFLEYGMKSGINPQEFHERLMPGLIRTGCISDAEDVPDDLKRLFTTALEIPYEQ
                     HIRIQAAFQKHVDNSVSKTVNMPESVSMADVEKAYLDAYRLGCKGITVFRYGSRKQQV
                     LEIGSDESTFEKEYFIKCDPGACRL"
     gene            complement(605..1072)
                     /locus_tag="VFJ67_04980"
     CDS             complement(605..1072)
                     /locus_tag="VFJ67_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289775.1"
                     /translation="MKILYVMAGALVCTLLLSAGATIDNALAGEAVGGIILQIDPEPE
                     ELDQVSEPVGDISEQPDDAPSESLDQVSETDDQASEEVGADIGSTEELDQASETDDQA
                     SEEVGADSETAEQASEVLPAAPEGEEYPLEEESPLQEEMPLGDREAEGRGLGQ"
     gene            1461..2348
                     /locus_tag="VFJ67_04985"
     CDS             1461..2348
                     /locus_tag="VFJ67_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HET7289776.1"
                     /translation="MKKLTGDKVEKIESPYEGISLKDYYIEKRRLQLELLLIQQSVAK
                     NGERWCICFDGRDAAGKGSTILRFTQNLMPKYYKIVDLGVPTPKESKYWFHRYEQHLP
                     NPGEMVFFDRSWYNRALIEPTMGYCTQKQYEYFMDKVLDWEHDLIDGGMKLVKFYLSV
                     DPEHQLYRFQERLTDPLKFWKFSENDYNARKKWETFTKYKEQMFAHTASEKSPWVLIN
                     SNSRKTSRLTCMLYVVRLYGNKKFVPLTGEDVTETHTIEINGVAFDGLNKLQYSVLKD
                     LADESELKAQEILTGPQNG"
     gene            2341..2811
                     /locus_tag="VFJ67_04990"
     CDS             2341..2811
                     /locus_tag="VFJ67_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7289777.1"
                     /translation="MDEDYTLANTLNLPRVRSAGGLVYNDNYHILLIFKRGKWDLPKG
                     RFEMGSDPHETALREISEETGLDKNKLTIQGKLVSTWHTTRHEKTKYLKKTHWYLVHY
                     HGEDSDVAPQFREGIIECRWVHLPDLDKYRPMLLPRIEYVIDFWLQNLAYAPRF"
BASE COUNT          750 a          790 c          801 g          656 t
ORIGIN      
        1 caggaacggc acgctcacat ccatagcgcc gacgggaact ataagcatca tcgccgggac
       61 gacctccgga atagagccgc tctttgcgct cgcgtacgaa agaaagaatg tgctccgagg
      121 ggagaccctc tatgaaataa acccgttgtt cctcgagtac ggcatgaaat ccgggatcaa
      181 tcctcaggaa ttccatgagc gtttgatgcc ggggctcatc agaaccggct gcattagcga
      241 tgccgaggac gtgcccgatg acctgaagcg attattcaca acggcgctcg aaatacctta
      301 cgaacagcat ataaggattc aggccgcttt tcagaaacac gtcgacaatt cggtttcgaa
      361 aacggtgaac atgcccgaga gcgtcagcat ggccgatgtg gaaaaagcgt atctcgatgc
      421 ctaccgtctc ggatgtaagg ggataacggt gttccgttac ggctcccgga agcagcaggt
      481 gctcgagatc gggtccgatg aaagcacgtt cgagaaggaa tattttatca aatgcgatcc
      541 cggcgcctgc aggctgtaat caaatgagtt gatcgggata atcgaggcgc gatatgcgtg
      601 tgtgttactg acccaggccc cttccctcgg cctccctgtc tcccagaggc atttcttcct
      661 gtaacggaga ctcctcttcg agcgggtact cctctccttc gggcgcggcc ggcaggactt
      721 cggatgcctg ctccgcagtc tccgaatcgg ctcctacctc ctccgacgcc tggtcgtcgg
      781 tctcagaagc ctgatccagc tcctcagtag aacctatgtc ggctcctacc tcttcggagg
      841 cctggtcgtc cgtctccgac acctggtcga gcgactcgga aggcgcgtcg tccggctgct
      901 ccgatatgtc gcccacgggc tcggatacct ggtcgagctc ctccggttca gggtctatct
      961 gcagaattat tccgccgacc gcttccccgg cgagagcgtt atcaattgtg gccccggcgc
     1021 tgaggagcag cgtgcacacg agcgcccctg ccattacgta taatattttc atacaacccc
     1081 ctttcatcaa cgtcttaaca attccttaac attgttccgt ataattctaa tgatagtgct
     1141 tatattcgga ttcggcaacg cttggccgcg gctgacgccg ccgtgggtgt ttcaggagaa
     1201 aacagcccgt tccgcgtccc ttttggatgg gtgcggaatt tgaaatatcg ggcttgtttt
     1261 tatataataa ttgacgcagc gaatccgacc tgccgaagtg acacaaaaca gtgttgtacg
     1321 taaatcttat catactgact ccctggattt aaataaaaaa ggggagaggt gcaactgctg
     1381 taagtcagct tcacggaaaa tattcagacg ggtcatttcg ggattgaccg gcttcaggga
     1441 ggtaacgtag gagaaactgc atgaaaaagc ttaccggcga taaagtagaa aagatcgagt
     1501 ccccatacga ggggataagt ctcaaggact attatataga gaagagacgc ctccagctcg
     1561 agctgctcct gatacagcag agcgtagcga agaacgggga gaggtggtgt atttgcttcg
     1621 acgggcgcga cgccgcgggt aagggctcga cgatactcag gttcacgcag aacctgatgc
     1681 cgaagtacta caagatagtg gacctcggcg tgccgacgcc gaaggaatcc aaatactggt
     1741 tccacaggta cgagcagcac cttcccaacc ccggggagat ggtgttcttc gaccggtcgt
     1801 ggtataaccg cgcgctcata gagccgacca tgggttattg cacgcagaag cagtacgaat
     1861 attttatgga caaggtgctc gactgggagc acgatctcat agacggcggc atgaagctcg
     1921 tgaagttcta cctttccgtc gatccggagc atcagcttta ccgttttcag gagcgtctga
     1981 ccgatcctct caaattttgg aaattttcgg aaaacgatta caatgcaaga aagaaatggg
     2041 agacgttcac gaaatacaag gagcagatgt tcgcccatac cgcgtccgag aaatcgccgt
     2101 gggtgctcat aaactccaat tcgcggaaga cgtcgagact cacctgcatg ctgtacgtgg
     2161 tcaggcttta cggcaacaag aagttcgtcc ctctgacggg cgaggacgtg acggaaacgc
     2221 atacgataga aattaatgga gtcgctttcg acggactcaa taaactacaa tattcagtgc
     2281 tgaaagatct ggccgacgag agcgagctca aggctcagga gatactgaca ggaccccaaa
     2341 atggatgaag attatacatt agccaatacg cttaaccttc cccgtgtcag gtctgcgggc
     2401 ggactcgttt ataacgacaa ctaccacatc ctccttatat tcaagcgcgg gaagtgggac
     2461 cttcccaagg gcaggtttga aatgggctcc gacccccacg agaccgcgct cagggagata
     2521 agcgaggaaa ccgggctcga taagaacaag ctgaccatac aggggaagct tgtttccacg
     2581 tggcatacga cgaggcacga aaaaaccaag tatctcaaga agacacactg gtacctcgta
     2641 cattatcacg gcgaggattc ggacgtcgcc cctcagttca gggaggggat catagagtgc
     2701 cgctgggtgc acctcccgga tctcgacaag taccgtccga tgctgctgcc gaggatcgaa
     2761 tacgtaatag acttctggct ccagaatctg gcgtacgccc ccaggttttg ataatcatta
     2821 cctgccggac acacactttg cggcggagtc tgtaaaggag cggaataaat tcggcgtgag
     2881 atggttggaa gaccactgcg ctcacggctg ccggctattt aaccagcttg ttgatccttt
     2941 ctttatagaa actattaacg aaatcgtcgg tatcgccgat gatctccacc cccattt
//
LOCUS       DASSJH010000232         4041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_165292, whole genome shotgun sequence.
ACCESSION   DASSJH010000232 DASSJH010000000
VERSION     DASSJH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165292"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1121
                     /locus_tag="VFJ67_04995"
     CDS             144..1121
                     /locus_tag="VFJ67_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289778.1"
                     /translation="MKRISNAAVSLTLVLFASCLVAAYLGQLSSQAAQSVRADAGGIQ
                     VAQAEDDEGGSSSSMGPRYTEAELISLDGEIFVNPLYTGKVSTVRTIDKNGLIGQKVD
                     IEVRIMTDGWRKFSLPQSASGAYGVIVSGDEGILKLSKPADAVTPAGGELKSIRKLVI
                     EFGYLNTVFSFPAMNVLEPSKVEIGVLDTSNNKFINGNIIAMKSDQAAVEFESLPSAV
                     VNKEGNMRVSLKEPGGTFINSDIPAWGYNIIVSETDIGSPAPIKAEVFGLPDDAEIRF
                     DFSSLTGQVIDPSTKILKVGEINKGAVVSTITTKIGGAQPLTVTVRRVK"
     gene            1201..1950
                     /locus_tag="VFJ67_05000"
     CDS             1201..1950
                     /locus_tag="VFJ67_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7289779.1"
                     /translation="MSGHSKWANIKHRKAAVDAKRGKVFTKLIRELTVAAKHGGGDPE
                     ANPRLRTAIAAAKTQNMANDTIDRAIKRGTGEMGGEEYHELSYEGYGPGGSAVLVKTL
                     TDNKNRTVSDIRRIFTKFGGSLGESGCVSWMFHMKGRIAFEKDKVDEDKIFEIALDAG
                     ADDVVTEESELVVITPPDAFEAVKSAIQQAGLSFENAEITMIPQNSVKIEGREAESMI
                     RLMEALEDSDDVQNVYSNFDVSEDLMLSLAS"
     gene            1958..2503
                     /gene="ruvC"
                     /locus_tag="VFJ67_05005"
     CDS             1958..2503
                     /gene="ruvC"
                     /locus_tag="VFJ67_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET7289780.1"
                     /translation="MIGIDPGSRVCGYGVLESRGGEVKYLTSGAIAPGNGLPLNQRLR
                     IIHESLMKVIGDHSPEAMSVEDIFFAKNAKSAIKLGEARGVALLAASSSGIPVFEYPP
                     TRIKLALTGRGRASKSEVRKMLSLILGVSDFETEDASDAVAIALCHIHLSRIEAKLGK
                     EFTQPRKRRRRFTLDDLPSKG"
     gene            2481..3068
                     /gene="ruvA"
                     /locus_tag="VFJ67_05010"
     CDS             2481..3068
                     /gene="ruvA"
                     /locus_tag="VFJ67_05010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET7289781.1"
                     /translation="MISLLRGKVAHKTLGKVVVDVNGVGYGVTVPLSTYYRLPETGSE
                     TELKIHTSMKDSSIELFGFHTEDERAVFTLLISVAGVGPRVATNILSYITPAEFASAI
                     SAGELERRKIPGIGPKLASRLTTELKDKVSGLGVSGEPSGKGGVLEDVVSALLNLGYK
                     KAEIDERISELERISAGETGIEAALRESLKVMRKL"
     gene            3151..>4041
                     /gene="ruvB"
                     /locus_tag="VFJ67_05015"
     CDS             3151..>4041
                     /gene="ruvB"
                     /locus_tag="VFJ67_05015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET7289782.1"
                     /translation="MAERLLSPRDGEDESLLDINLRPDRLRDYLGQVKVKEKVGIFIE
                     AARRRKEALDHVLLYGPPGLGKTTLAHVVSNELGVRIYATSGPVIERVGDLASILTNL
                     EDRDVLFIDEIHRLNRIVEEYLYSAMEDYKIDIMIGEGPTAKSMKVSVNRFTLVGATT
                     RTGLLTSPLRSRFGVDLRLDYYTSEEIEEILKRSAGILGVEITSGGVAELACRARGTP
                     RIANRLLKRVRDFAEVRADGVIDTGVAGSALEMLEVDSLGLDNLDRAILETIIEKFRG
                     GPAGIDSIAAAVSEDSDTIEE"
BASE COUNT          989 a         1034 c         1234 g          784 t
ORIGIN      
        1 ttttcggtaa taataaaacc cctcgacagg tctcaggcat cggggcgcgt taaaataccc
       61 ggctgtatat gctataatca aattttaagt tatccgcgcc cgatttttta gaacggttaa
      121 aaatgcttat tacagaggga gttatgaaac gtatatccaa tgccgcagta tccttgaccc
      181 tcgtattgtt cgcttcgtgc ctcgtggcgg cttatctagg ccagctttcc tcccaggcgg
      241 ctcaatccgt ccgggccgat gcgggaggca tccaggtcgc tcaggcggaa gacgacgaag
      301 gaggctcttc gtcatccatg ggtcccaggt acacggaagc cgagctcatt tcgctcgacg
      361 gagagatatt cgtaaaccct ctatacacag gaaaggtctc aaccgtgagg acgatagata
      421 aaaacgggct cataggacaa aaggtcgaca tagaggtcag gataatgacg gacgggtggc
      481 ggaagttcag cctccctcag tcggcgtcag gggcgtacgg ggtcatagtc tcgggcgacg
      541 agggaatact gaagctctcg aaaccggccg acgccgtgac tcccgcggga ggggaattga
      601 agtcgatacg gaagctcgtt atcgagttcg gatacctgaa taccgttttt tcattcccgg
      661 ccatgaacgt gctcgagccg agcaaggtgg agataggagt gctcgacacc tcgaataaca
      721 agttcataaa cggcaatatc atagccatga agtccgatca ggccgcagtc gagttcgaaa
      781 gcctgccttc ggccgtggtg aataaggaag gcaatatgag ggtgagccta aaggagccgg
      841 gcggcacctt tatcaattcc gatatcccgg cctgggggta taacataatc gtatcggaga
      901 cggacatcgg cagccccgcc cctataaagg ccgaggtctt cggcctgccg gacgacgccg
      961 agataaggtt cgacttcagc tcgctcacgg ggcaggtgat agacccctcg actaagatac
     1021 tcaaggtggg ggagataaat aaaggggcgg tcgtctccac gataacgacc aagatcggag
     1081 gcgcccagcc tctcacggtg acggtgagga gggtgaaatg acgcccttgc cattcattat
     1141 ccaatgtctt agaattctta tatagtgtat tttaacaatc ggaagtgagg tgatgtaacc
     1201 atgtccggac attcaaaatg ggcgaatata aaacacagaa aggcggctgt ggacgccaaa
     1261 aggggtaagg ttttcacgaa gcttatacgt gaactgaccg ttgcggccaa gcacggcggg
     1321 ggggaccctg aagcgaaccc tcgtctcagg accgcgattg cggctgccaa gacccagaat
     1381 atggcgaacg acaccataga ccgggccata aagcggggca cgggcgaaat gggcggggaa
     1441 gagtatcatg aattatccta cgagggttac gggccgggcg ggagcgccgt cctggtgaag
     1501 acgcttaccg acaacaagaa caggaccgtt tccgatatca ggaggatatt cacgaagttc
     1561 ggcggaagcc tgggggagtc ggggtgtgtt tcctggatgt ttcacatgaa gggaaggatc
     1621 gcgttcgaga aggacaaggt cgatgaggac aagatattcg aaatcgctct cgacgcgggg
     1681 gccgacgatg tcgttaccga agagagcgag ctcgtcgtta tcaccccgcc cgacgcattc
     1741 gaggcggtga aatccgcgat acagcaggcg ggcctgagct tcgagaacgc tgagatcacc
     1801 atgatcccgc aaaacagcgt gaagatcgaa ggcagggaag ccgagagcat gatacgtctc
     1861 atggaggctc tcgaagacag cgacgacgtt cagaacgtgt attcgaactt cgacgtatcg
     1921 gaagacctca tgctgagcct ggcgtcctga gatgagggtg atcggaatag accccggttc
     1981 gagagtttgc ggctacgggg tccttgagtc ccgcggcgga gaggtcaaat acctgaccag
     2041 cggtgccatc gctccgggaa acggccttcc gctcaatcaa agactgagaa tcatacacga
     2101 gtcactgatg aaggtgatcg gcgatcactc gcccgaggcg atgtccgtcg aggatatttt
     2161 ttttgcgaaa aacgcgaaga gcgcgataaa gctcggggag gcgagaggcg tagcgcttct
     2221 ggccgcgtcc agttcgggta taccggtatt cgaatacccg ccgaccagga taaagctcgc
     2281 gctcacgggc aggggcaggg cgagcaagtc ggaagtaagg aagatgctct cgttgatact
     2341 cggagtctcc gacttcgaga ccgaggacgc ttcggacgcc gttgctattg cgctctgcca
     2401 tatacatctc tcacgaatcg aagctaaatt aggaaaggaa ttcacacagc cgcggaagag
     2461 gaggaggcgc ttcactctag atgatctccc ttctaagggg taaggttgcc cacaagacgc
     2521 tcgggaaggt ggtggttgac gtgaacggcg tcggatacgg tgtgacggtg cccctttcca
     2581 cctattacag gctccctgaa acggggagcg agaccgaatt gaagatccac accagcatga
     2641 aggattcctc tatcgagctc ttcggctttc acaccgagga cgaacgagcc gtattcacgc
     2701 tccttattag cgttgccggg gtcggtccca gggttgcgac gaacatactt tcatatatca
     2761 caccggcgga gttcgcatcc gctatatccg cgggcgagct ggagaggcgg aaaataccgg
     2821 gcatcggacc gaagctcgcc tcacgcctca cgaccgagct caaggacaag gtctcgggct
     2881 tgggcgtgtc tggggagcct tcaggtaaag gcggcgtcct cgaagacgtc gtatccgctc
     2941 tccttaacct cggatataaa aaggccgaga ttgacgagcg catctccgag ctcgagcgca
     3001 tatccgcggg cgaaaccggc atagaggcgg ctctcaggga atcgctcaag gtcatgagga
     3061 agctgtgagc ttctttccgt gatccggatt tctaagaatc gtctcttaat ttgtattata
     3121 atagatttac tccatagaga gggagcaaga atggcggaaa ggcttctgag cccgagagac
     3181 ggggaagacg agtcgctcct cgatataaac ctcaggcccg accggctgag agactatctc
     3241 ggtcaggtga aggtcaagga gaaggtgggg atcttcatcg aggccgcgag gaggaggaag
     3301 gaggcgctcg accacgtgct cctttacggc ccgcccgggt taggcaagac gacacttgcg
     3361 cacgtcgtct ctaacgagct cggcgtcagg atctacgcca cctccggccc tgttattgag
     3421 agggtcggcg acctcgcatc catactcacg aacctcgaag acagggacgt gctgttcata
     3481 gacgagattc accgcctgaa ccgcatcgtt gaggaatacc tctactccgc gatggaggac
     3541 tacaagatag atatcatgat aggcgaaggg ccgacggcta aatccatgaa ggtgtcggtg
     3601 aacaggttca cgctcgtagg cgcaacgacg aggacgggtc tcctgacctc tccgctcagg
     3661 tcgaggttcg gcgtcgatct ccggctcgat tactatacgt ctgaggagat agaggagata
     3721 ctgaagcggt cggccggcat cctcggcgtg gagatcactt cgggcggagt ggcggagctc
     3781 gcatgccgcg cccgcggcac tccccgcata gcgaacaggc tgctgaaaag agtgagagac
     3841 ttcgccgagg tccgggcgga cggcgtaatc gataccggcg tcgcgggctc cgcgttagag
     3901 atgctcgagg tcgattccct cgggctcgat aacctcgaca gggcgatact agagaccata
     3961 atcgaaaagt tcaggggcgg ccctgcgggt atagattcca tcgccgcagc ggtgagcgaa
     4021 gacagtgaca ccatagagga g
//
LOCUS       DASSJH010000233         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1355196, whole genome shotgun sequence.
ACCESSION   DASSJH010000233 DASSJH010000000
VERSION     DASSJH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355196"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFJ67_05020"
     CDS             complement(<1..528)
                     /locus_tag="VFJ67_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289783.1"
                     /translation="MSAMKQMENPPKARLAVRVGVTGHRPHGLDRADIGTLKIKVNEV
                     LKHVKGVAVEVKSSFESLYADGGPPILRIISPLAEGADMLVAEEALAEGYELQCVLPF
                     DRQEYEKDFTDGDSLGKYRELLEKATALLELDGSRVTPDLENEAYQTAGRMVLAQSDV
                     LIAIWDGEDEKGKGGT"
     gene            complement(535..2388)
                     /locus_tag="VFJ67_05025"
     CDS             complement(535..2388)
                     /locus_tag="VFJ67_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289784.1"
                     /translation="MPQINEDYLIPIVVGVSGRKGSQPAGERISSLVIPEMHGLRNTF
                     PSSPFVVISSLSGSEDRLLARLAVEHLRANLIAVLPSPPDQYGEALPDDKSREEFSEL
                     LGQAGSLVDIASLMRSRLGETTYATAENPEMTGRFAAAFIAEHSEILFAVSSDGDTRE
                     PDASALIVKWMRDGGITREYSLRVNKSGLDTMDRRMIITIDPAGSGAGVEYPPPDEAA
                     ARGISGPEKPGSFPGVVYRAVSRQVFGLIQSVSGMLDDLYDSFFKHTGPGVADRFERS
                     PFTGIMERIDGFNLDIRNYLDTRGEKDLLKSRRYLLQDESDNDRISGDPALRKIICLY
                     TGSDVLSQYYQKKTASLVNWIYVLFFAAVVLYGLIDWSSYLVLCYIGLIPAIALLVKK
                     TSRDRVEDRFLDYRALAEGLRVQFFWTLCGINENVSSHYLSKYEGLLTWIRKAVRSIE
                     IVTLQNESAEAERQQNEFLVVTNRLWIESQLGYFSSKKRPLLVRTQQFGNVVFLSFVL
                     TLIVALAYGVYVLAAGVGNGEAINDFQILLGVIAAVGVAAQAYKNKRAYDELQRRYSL
                     AQQTYASAKRELEIGKVPPERVLIAVGREALLENSDWLWTHRNVPIEVPKG"
     gene            2815..3216
                     /locus_tag="VFJ67_05030"
     CDS             2815..3216
                     /locus_tag="VFJ67_05030"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HET7289785.1"
                     /translation="MKKQKLLILLLLAACPFILTGCLAAAAVGGAAAAGAGTVAYIKG
                     ELKATEEATLNKTWEATVAAVDDLQFIVINKIKDDVSGQLEAKTADNKTVKIQLTRVT
                     SNLTDISIRIGTFGDESLSRYILSKIEARLK"
     gene            3322..3687
                     /locus_tag="VFJ67_05035"
     CDS             3322..3687
                     /locus_tag="VFJ67_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017291805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7289786.1"
                     /translation="MISREKALRIARDWIDSWNRHDLDSIMSHYSEDVELISPIVIKL
                     LGDESAKVTGRDALREYFKKGLAAYPDLRFETIDVFTGVDSLVLYYRRHNGPPGAELM
                     VLNQEDKITRVIAHYSYAE"
     gene            complement(3719..>3988)
                     /locus_tag="VFJ67_05040"
     CDS             complement(3719..>3988)
                     /locus_tag="VFJ67_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009843890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HET7289787.1"
                     /translation="VMRVFFYMPFMHSESKDMQNRSMALFGALEKGFTYPRELAEMLS
                     DNMEYAERHRAIIERFGRFPHRNRILGRESTTEEIEFLKQPGSSF"
BASE COUNT          927 a         1123 c         1017 g          921 t
ORIGIN      
        1 cgtgcctccc ttccctttct cgtcctcacc gtcccatatc gcgataagca cgtcgctctg
       61 cgccagcacc atacggccgg ccgtctgata ggcttcattc tccagatcgg gagtcacccg
      121 cgaaccgtcg agctcgagca gagcggtggc tttctcaagc agctcacggt acttcccgag
      181 agaatcgccg tccgtaaaat ccttctcgta ttcctgccgg tcgaaaggga gcacgcactg
      241 gagctcgtat ccctcggcga gggcttcttc ggcgacgagc atgtccgcac cctcagcaag
      301 gggcgatatt atcctgagta tcggcggtcc cccgtcggca tataaagatt cgaaagacga
      361 tttcacctcg acggcgacgc cctttacatg cttcaggacc tcgttcacct ttattttgag
      421 tgtaccgata tcagccctgt cgaggccgtg aggacggtga cccgtaacgc ctaccctgac
      481 cgcgagacgg gctttcgggg gattttccat ctgcttcatt gcgctcataa cattctaccc
      541 tttgggtacc tctatgggca cgttcctgtg ggtccacagc cagtcgctgt tctcgaggag
      601 cgcctccctg cccacggcga tgagaaccct ctccggcggc accttgccga tctcaagctc
      661 cctcttggcc gacgcgtagg tttgctgagc caggctgtac ctcctttgga gctcgtcata
      721 cgccctttta ttcttatatg cctgggcggc tactcccaca gcggcgatca cgcccagaag
      781 gatctggaaa tcatttatgg cctcgccgtt tcccacgccc gcagccaata catatacgcc
      841 gtaagccaga gcaactatca aagtcaatac gaacgacagg aatacgacgt tgccgaactg
      901 ctgtgttctt accagcagcg gccttttctt cgagctgaaa tatccgagct gagattctat
      961 ccataatcta ttggtgacga cgaggaattc gttttgttga cgctcagctt cggcgctttc
     1021 gttttgaagt gtcacgatct cgatgcttct taccgccttc cttatccatg tgagcaagcc
     1081 ctcgtacttg ctcaggtagt gggaagacac gttctcgttt atcccgcaga gcgtccagaa
     1141 gaactgcaca cggagtcctt ccgcaagcgc cctgtaatcg aggaagcggt cttccaccct
     1201 gtctctcgag gtctttttta caagaagcgc aatcgcgggg atgagcccta tgtagcagag
     1261 gaccaggtaa gagctccagt cgatgagccc atagagcaca acggctgcga aaaagaggac
     1321 gtagatccag ttgacgaggc tcgcggtctt cttctgataa tactgcgaca gcacatccga
     1381 acccgtgtag aggcagatta ttttacggag cgccgggtcc ccggatattc gatcgttatc
     1441 cgattcgtcc tgaagcagat agcgcctgga tttcaggagg tctttttcac cccgggtatc
     1501 gagataattc cttatatcca ggttgaagcc gtcgatcctc tccataattc ctgtaaaggg
     1561 cgatctctcg aatctatccg cgacaccggg tcctgtgtgc ttgaaaaaag aatcgtacag
     1621 gtcgtcgagc atgccgctca ccgactggat cagaccaaaa acctgcctgg aaaccgctct
     1681 ataaacgacg ccgggaaagc tgccgggttt ctccgggccc gaaattcccc gggccgcggc
     1741 ttcgtcgggc ggcgggtatt ccactccggc cccggagccg gccggatcga tagtgattat
     1801 catcctcctg tccatggtat ccaggccgga tttattaacc ctgagggagt actcccgcgt
     1861 tatgccgcca tcacgcatcc atttgactat caaggccgat gcatccggct cgcgcgtatc
     1921 accgtcgctt gaaactgcaa aaagaatttc gctgtgctcg gcgataaacg ccgcggcgaa
     1981 tctccccgtc atttccggat tctccgccgt ggcgtaagtt gtttccccga gcctcgacct
     2041 catcagcgat gcgatatcga ccaacgaccc ggcctggccg agaagctccg agaactcttc
     2101 cctgctttta tcgtccggga gcgcctcccc gtattgatcg ggcggagacg gaagtaccgc
     2161 tataagattt gcgcgcaggt gctcgaccgc aagtctcgcc aacaggcggt cctcgcttcc
     2221 tgagagagag gatatgacaa cgaacggtga gctggggaac gtattgcgga gcccgtgcat
     2281 ctcgggaatg acgagggagg agatacgctc tccggcgggt tgtgagcctt tccggccgct
     2341 cactccgacc acaatcggta tcagatagtc ttcatttatc tgcggcataa tagagtttca
     2401 atcagagttc gtgtgctttt cccttgaacc agccgctgta ccgacgtccg cgccgcattt
     2461 ttatacacgg cttattcggc aacaaccccc cagttcccgc cctcgattaa caatatacga
     2521 ttgagctcga tcgaagtttc gtttccgaag caaaatcttt aattggtttt ctccgtcaga
     2581 accggaaacc gcttgacccc gatgatatta caattctaat tatgagtaaa taataattca
     2641 acagttcttc cggcgacaga tccatgcagg atactacatc agacacggcg ctcacccggt
     2701 taatccccgt gaatggaaca atacagttgc tttgttccgc cgtcacagga tataattgaa
     2761 ttatttgcac atcatagtcc tgacaagacc ttttgcaccg gaggaatcac cgatatgaag
     2821 aagcagaagc tattgatctt actattactt gcggcttgtc cttttattct gaccggatgc
     2881 ctcgcggcgg cggccgtagg cggcgcggcg gcggcgggcg ccgggactgt agcctatata
     2941 aagggagagt tgaaggcgac cgaagaggcg actctcaaca aaacatggga ggccaccgtt
     3001 gcagcggttg acgatctcca gttcatcgtg ataaacaaga taaaagacga tgtttcggga
     3061 cagctcgaag ctaagaccgc cgacaacaag accgtcaaga tacaattaac cagggtcacg
     3121 tcaaacctga ccgacatatc gataaggatc ggaacgttcg gtgatgaaag cctttcgcgc
     3181 tatattctga gcaagataga ggcgaggctc aagtgacccc cgggtccgaa tccggtatag
     3241 agcagggtga agaacggtta tgcaaacggc tgccggatga aaccgtaacc atgacttgat
     3301 tcaggactta aaccgaaata aatgatttcc cgcgaaaaag ccttacggat cgccagggac
     3361 tggatcgatt cctggaacag gcacgacctc gattccatca tgtcccacta ctcggaagac
     3421 gtggagctca tatcaccgat cgtgataaag ctccttggcg acgaatcggc taaggtcaca
     3481 ggcagggacg cgctgaggga gtacttcaaa aaggggctgg ctgcgtatcc cgacctccgg
     3541 ttcgagacga tcgatgtctt cacaggcgtc gacagcttgg tgctttatta caggagacat
     3601 aacgggccgc ctggagcaga gctgatggta ttgaaccagg aagataaaat cacgagagtc
     3661 attgcccatt acagctatgc ggaataagtg cgagcccgca tagcccgtag aagattgatc
     3721 agaaagaaga gccgggctgt tttaaaaact cgatctcttc agtcgtagat tccctgccga
     3781 gtatcctgtt ccggtgggga aatctcccga aacgctctat tatcgcacgg tgtctttccg
     3841 cgtattccat attgtctgag agcatttcag cgagctcgcg agggtaagtg aaacccttct
     3901 ccagggcccc gaaaagagcc atcgatctat tctgcatatc tttactctct gagtgcataa
     3961 acggcatata gaagaaaacc ctcattac
//
LOCUS       DASSJH010000234         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_382100, whole genome shotgun sequence.
ACCESSION   DASSJH010000234 DASSJH010000000
VERSION     DASSJH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382100"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /gene="pdxA"
                     /locus_tag="VFJ67_05045"
     CDS             complement(<1..915)
                     /gene="pdxA"
                     /locus_tag="VFJ67_05045"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET7289788.1"
                     /translation="MSKKPRIGLTMGDPNGISPEIVVKALNSDEVTGICSPIIYGSGD
                     VLESAAKLTGLKLDCPIHDPSGFRTADLKPCRVDRKAGEASLSYIKEAVKAAMSGELD
                     AVVTAPISKESTHLAGSKFPGHTEMLKELTGAEEAVMMFEGGSFRVVLVTIHTALREV
                     PNLITKERVLSVIKITHRALADKFKIPQPRIAVCGLNPHAGESGAFGDEEIVHITPAV
                     AEARKLGIGVTGPLPADTLFYYANQGKWDAVVAMYHDQGLIPFKMLSFNVGVNLTLGL
                     PIIRTSPDHGTAFNIAWKGVADPSSMIAA"
     gene            complement(976..1737)
                     /locus_tag="VFJ67_05050"
     CDS             complement(976..1737)
                     /locus_tag="VFJ67_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7289789.1"
                     /translation="MLIDTHAHLDSLEDLTGTLARAREYGVEKIIAISSDLASSRDTV
                     SIAGKNENVFAAVGIHPHAARTADAAVLKEIETIAGKEKVVAVGETGLDYHYMNSTRE
                     EQIHSLTAHIAIANRAALPIVIHVRDADPDLMEILRKEKVSDRTGVIHCFTGNYETAR
                     KYIDLGFYISFSGIATFKKSEDIRDAAKNIPADRLLLETDSPYLAPVPMRGKPNEPAY
                     VKYVAEAVAAARGVTFEEISELTAENAERLFKLGR"
     gene            complement(1821..2270)
                     /gene="nrdR"
                     /locus_tag="VFJ67_05055"
                     /pseudo
     CDS             complement(1821..2270)
                     /gene="nrdR"
                     /locus_tag="VFJ67_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647752.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
     gene            complement(2271..>3251)
                     /gene="glyA"
                     /locus_tag="VFJ67_05060"
     CDS             complement(2271..>3251)
                     /gene="glyA"
                     /locus_tag="VFJ67_05060"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7289790.1"
                     /translation="QPHSGAQANMSVYFACLEPGDTILGMNLAHGGHLTHGSPVNFSG
                     RLYNVVPYGVTEDTNLIDYDEVRRLALEHRPKLIIAGWSAYPRDIDFGRFRQIADEAG
                     ALLMADIAHPAGLVVAGLYSNPVPHCDFVTTTTHKTLRGPRGGMVMMKGSQEKQVNSR
                     VFPGVQGGPLMHVIAAKAVAFKEALDPGFKEYQKQIVRNSKKLGECLTGMGLRLVSGG
                     TDTHLVLVDLRETELTGKIAEETLEAAGITVNKNAIPFDPRPPAVTSGIRIGTPAITT
                     RGMKEKEIEIIAGFIKEALNNPGNEKVLSRIKDDVRVLCSQFPIYRHRLI"
BASE COUNT          567 a          961 c          868 g          855 t
ORIGIN      
        1 ggcggcgatc atactcgaag gatcagcgac ccctttccac gctatattga atgcggtccc
       61 gtgatcgggc gacgtcctta ttatcggaag cccgagcgtc agatttacgc cgacgttgaa
      121 tgagagcatc ttgaaaggga tgaggccctg gtcgtggtac atcgcaacga cggcgtccca
      181 tttaccctga tttgcgtaat aaaagagcgt atccgcaggc agcggccctg taacgccgat
      241 cccgagcttc cttgcttcgg ctacggccgg ggtgatgtgg acgatctcct cgtcgccgaa
      301 agcgcccgac tccccggcgt gggggttcag gccgcagacc gctatcctgg gctgcgggat
      361 cttgaattta tcggcgagcg cgcggtgagt gatctttatc acggaaagca cgcgctcttt
      421 cgttatgagg ttaggcacct ccctgagcgc ggtgtgaatt gtgacgagca cgaccctgaa
      481 gctccctccc tcgaacatca taacggcctc ctcagcacct gtgagctctt tcagcatctc
      541 cgtgtgcccg gggaacttcg accccgccag gtgagtggat tccttgctta taggcgcggt
      601 gacgaccgca tccagctccc ccgacatcgc ggctttcacc gcctctttta tgtatgatag
      661 actggcctcg ccggcttttc tgtccaccct gcagggcttg aggtcggctg tgcggaatcc
      721 ggacggatcg tgtatcgggc aatccagttt caggcccgtc agcttggccg ctgactcgag
      781 aacatcaccg ctcccgtaga ttatggggga gcagatccct gtcacctcat ccgagttgag
      841 ggcctttacc acgatctccg ggcttatacc gttcgggtcg cccatggtca atccgattct
      901 aggcttcttg ctcatcaagg gattatttta aataaacgta ttcgccgtgt atagggtgac
      961 gggcgtgagc ggcgctcacc ttccgagctt gaaaagcctc tcagcattct ccgcggtcag
     1021 ctccgagatc tcttcaaacg tgacgcctct cgcggcggcg accgcctccg ctacgtattt
     1081 cacgtatgcg ggctcgttcg gttttcccct catagggacg ggagcgaggt atggggagtc
     1141 cgtttccagg agcagcctgt cggcggggat attcttcgcc gcgtcccgta tatcttccga
     1201 tttcttaaac gtcgctatac cggagaatga aatgtagaac ccgaggtcga tgtattttct
     1261 ggccgtctca tagtttccgg tgaagcagtg gatgacaccg gtcctatccg agaccttttc
     1321 tttcctgagt atttccatga ggtcggggtc ggcatccctc acgtgtatta ctatcggcag
     1381 cgctgcgcgg ttcgcgatgg cgatgtgcgc cgttagggaa tgtatctgct cttcccttgt
     1441 agaattcatg tagtggtagt caagccctgt ctcacccacg gccacgacct tctccttgcc
     1501 cgcgatcgtc tctatctcct tgagaacggc ggcgtcggct gtcctggcgg cgtgcgggtg
     1561 gatgccgaca gcggcgaata cgttttcgtt cttcccggcg attgacacgg tgtcgcgcga
     1621 ggacgcgagg tccgaggaaa tggctatgat tttttcgacc ccgtactccc ttgccctcgc
     1681 gagagtaccc gtcaggtctt caagcgagtc caggtgggcg tgcgtatcga taagcattac
     1741 ttcttggttt tgtcctttat cagcgttagc gtttgtaagg tgggatcggt cttacggcct
     1801 aattttatct gctcaccttt ctccttgagg agatcggtga gctcgttcag gaactggttc
     1861 acgtctttaa acgacctgta aacggaagcg aatctcacat acgccacttc gtctatttcg
     1921 tagagcttgt ttatcaccct ttccccgatc tcgctgctgt ccacttccct gtcgcccctc
     1981 tcctggaact ctctttcgat gtcggagacg aagttctcga ttacttccat gcttatgggc
     2041 ctcttctcgc acgccttgat catacccgcg attatcttgc tgcggtcaaa tggctcccgc
     2101 accccgcctt tctttatgac gaggagctcc atttcctcga ccctttcgta agtcgtgaac
     2161 ctgctcttgc agttaaggca ttccctgcgt ctccttatcg acgtatcttc cttggcgaga
     2221 cgcgagtcaa tgaccttact ttccaggctc gagcagaatg gacatttcat ctatatcagt
     2281 ctgtgtctgt aaatcgggaa ttggctgcaa agcacccgga cgtcatcctt tattctcgaa
     2341 agcacctttt cgttacccgg gttgttcaat gcctctttta tgaaaccggc gattatctct
     2401 atctcttttt ccttcatgcc cctggtagtt attgccggcg ttccgatcct tatgccgctc
     2461 gttactgccg gcgggcgcgg gtcaaacggt attgcgttct tgtttactgt tatgcccgca
     2521 gcctctagtg tttcctcggc gatctttccc gtaagctctg tctccctgag gtctacgagc
     2581 accaggtgag tgtccgtgcc tcccgagacg agcctgagcc ccattcccgt gaggcattcg
     2641 ccgagcttct tcgaattcct gacgatctgt ttctgatatt ccttgaagcc ggggtcaagc
     2701 gcctctttga acgcgacagc ctttgcggcg attacgtgca tcagaggccc cccctgtacg
     2761 ccggggaaaa ccctgctgtt gacctgcttt tcctggcttc ccttcatcat gaccattccg
     2821 cccctgggcc ctctcagggt cttgtgagtg gtcgtcgtta cgaaatcgca gtggggcacc
     2881 gggttcgaat acagccccgc gaccacgagt cctgccgggt gtgcgatatc cgccataaga
     2941 agggcgcccg cttcgtctgc tatctgcctg aacctgccga agtctatgtc cctcgggtag
     3001 gcgctccatc cggcgattat gagcttcggc ctgtgctcga gcgcgagcct ccttacctcg
     3061 tcgtaatcga tgaggttcgt atcctcggtc accccgtacg ggactacgtt ataaagcctc
     3121 cccgagaagt tgacggggct tccgtgggta aggtgtccgc cgtgagcgag gttcattccc
     3181 agtatggtgt caccgggctc aaggcaagcg aagtaaaccg acatattcgc ctgtgcgccc
     3241 gagtgcggct g
//
LOCUS       DASSJH010000235         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1569083, whole genome shotgun sequence.
ACCESSION   DASSJH010000235 DASSJH010000000
VERSION     DASSJH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569083"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="VFJ67_05065"
     CDS             complement(<1..1617)
                     /locus_tag="VFJ67_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase"
                     /protein_id="HET7289791.1"
                     /translation="MSTVRDISKQPFKVVSAPRSLQIRNSLESEYADIYTPQVLEALK
                     ALSVFNRDQKLLMEKRYARRAQRNRNRERIRFLNPNDFIPRTNIKVQDARDGKFVGSE
                     IPHDLQRQWIQGTGPAAKPNTSLDKSIRNVAYALLSGADGWMFDGEDALGQISTMSLD
                     NQRNLKLAIRKDPVFMNVAEQVSREMNEWAKGFFGREIIMDWRKQLDFTTKIFRARGL
                     HLDDRHIRDENGLMLSASVVDVVLYVVNNYQELDKSGSSIVLYLPKIQTAEEAAFWNE
                     LLSALEDHLSIPLGSIKVYVLIEQLEATFQLMEIRAALGRHFVGFNTGRWDYINSVSD
                     AMAWDQSFINPNIESITMTYGYMRNYEDRVRRAVNTPGVNHNYALWQGGMEPNIPVGS
                     DKGVKEGMKKAVEGAKREQQEGASGKWVAHWKMVHIVRPVWEKQGQENQLGRQFPALT
                     YTEEDAAGLRAVEPSPRTVRGARDLASVAMQYGNAFGRGFQAAALKPADSFGNDDVLY
                     LMEDMATGEIRLSILWEWVHKGARLTADDVE"
     gene            complement(2054..>2341)
                     /locus_tag="VFJ67_05070"
     CDS             complement(2054..>2341)
                     /locus_tag="VFJ67_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002753539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7289792.1"
                     /translation="MDDINPEFPDADVALVIGANDVTNPAARHDKSSPIYGMPILDVD
                     KAKHIMVIKRSMNPGFAGIENELYFDEKTMMVFGDAKTVVGNITKELNELK"
BASE COUNT          439 a          679 c          633 g          590 t
ORIGIN      
        1 ctcgacgtcg tctgccgtga gtcgcgcgcc cttgtgtacc cattcccaca ggatgctcaa
       61 ccgaatctcg cccgtagcca tgtcctccat caggtagagg acatcgtcat tgccgaatga
      121 gtcggcaggc ttgagcgcgg ctgcctggaa gccgcggccg aaggcgtttc catactgcat
      181 cgcgacgctc gccagatcgc gcgcgccccg taccgtgcgg ggtgagggtt ccacggcccg
      241 caggccagcg gcgtcctcct ccgtgtaggt gagcgcgggg aactggcgtc cgagctggtt
      301 ctcctggccc tgcttctccc agaccggccg gacgatgtgg accatcttcc agtgagcgac
      361 ccacttgccg ctcgcgcctt cctgctgttc ccttttcgcg ccttcgacgg cctttttcat
      421 cccttcctta acgcccttgt ccgagcctac ggggatgttg ggctccatgc cgccctgcca
      481 gagcgcgtag ttatggttta cgccgggggt gttcacggct ctcctgacac ggtcctcgta
      541 attcctcatg tagccgtaag tcatcgttat cgattctatg ttggggttga tgaacgactg
      601 gtcccacgcc atcgcgtcgg aaacgctgtt gatgtagtcc cacctgcctg tgttgaatcc
      661 gacgaaatgc ctgccgagcg cggcgcgtat ctccataagc tggaacgtgg cttccagctg
      721 ttcgatgagc acgtacacct ttatagatcc gagcgggatg ctcaggtggt cctccagcgc
      781 agacaagagc tcgttccaga acgcggcctc ttcggccgtc tgtatcttcg ggagatagag
      841 cacgatagac gagcccgatt tatcgagctc ctgatagttg ttcactacgt aaagcacgac
      901 gtcgacgacg gaagccgaga gcatgaggcc gttctcgtcc cttatgtgcc tgtcgtcgag
      961 gtggagcccc ctcgccctga atatcttcgt cgtgaagtcg agctgctttc tccagtccat
     1021 gattatttcg cggccgaaaa atcccttggc ccattcgttc atctctctcg agacctgctc
     1081 ggcgacgttc atgaacaccg ggtctttcct tatcgcgagt ttcagattcc tctggttgtc
     1141 gagagacatg gtggatatct gtccgagcgc gtcttcgccg tcgaacatcc agccgtctgc
     1201 gcctgaaagg agggcgtaag cgacgttcct tatgctcttg tcgagggacg tattcggctt
     1261 ggccgccggg cccgtgccct ggatccactg cctctggagg tcgtgcggta tctcggagcc
     1321 cacgaacttg ccgtcgcgcg cgtcctgcac ctttatatta gtacggggta tgaaatcgtt
     1381 ggggttgagg aaccttatcc tctccctgtt gcggttgcgc tgggcgcgcc tcgcgtacct
     1441 cttttccata agcagcttct ggtccctgtt gaacacggaa agcgctttca gggcctccag
     1501 tacctggggg gtataaatgt ccgcgtactc gctttcgagg ctgttcctta tctggaggct
     1561 cctcggggcc gagaccacct tgaacggctg tttagaaata tcacgaacgg tgctcatgtt
     1621 gctccttctc cttgggttat tttctgtgca aaagatttaa aatgggaacc atcattataa
     1681 tacacccggt cttcaaatca actaaaatta aaataacctg ccgtactgat taatatattt
     1741 tcctcaacgg agtaatattg cgaccgggcc ggagaaaaaa atctgttctg cgacgcacaa
     1801 cagacgaacg aaggttatcg atagagaacc ctgcccctta aaaacatttt acccgttttt
     1861 atgcccttgt gaattgtccg gggataaatt gcggcgagtc gccaatattc ggaagtaata
     1921 aatgttcaac ccttcgacaa gctcacggtt tcgtcaagac ttcgccggac aggaggaacg
     1981 aacgtgtttg gctttgatgc cggtttggca gaggagcctc acgagaaccg taaatcagca
     2041 gacattgtct gccttactta agctcgttga gctccttcgt gatattaccg acgaccgtct
     2101 tcgcgtcgcc gaacaccatc atggtcttct cgtcgaagta tagctcgttc tcaatacccg
     2161 cgaacccggg gttcatgctc cgcttgatga ccattatgtg cttggccttg tccacgtcca
     2221 gtatcggcat gccgtatatc gggctcgact tgtcgtggcg cgcggcgggg ttggtaacgt
     2281 cgtttgcccc tatcacgagc gcgacgtcgg cgtccgggaa ttcggggttg atgtcgtcca
     2341 t
//
LOCUS       DASSJH010000236         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_918414, whole genome shotgun sequence.
ACCESSION   DASSJH010000236 DASSJH010000000
VERSION     DASSJH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918414"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFJ67_05075"
     CDS             <1..314
                     /locus_tag="VFJ67_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET7289793.1"
                     /translation="NILGVPKDTLDTYGAVSAPVVEAMAHGVRKLAGSDIGLAVSGIA
                     GPGGGTPEKPVGTVFIGIDSGKGGTRSKKFLFHGTREEIKLVTSSHALRLIMQIFLNN
                     V"
     gene            327..1367
                     /gene="recA"
                     /locus_tag="VFJ67_05080"
     CDS             327..1367
                     /gene="recA"
                     /locus_tag="VFJ67_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET7289794.1"
                     /translation="MAKQTFSPDSSEKEKTIELAISSIEKQFGKGSIMRLGAEAPIPQ
                     LSVISSGSLGLDIALGVGGYPRGRIVEIFGPESSGKTTLALHVIAEAHKKGGIAAFVD
                     AEHALDPNYAKNLGVKIDDLLISQPDFGEQALEIVDTLVRSGAVDVIVLDSVAALVPR
                     AEIEGEMGDAHVGLQARLMSQALRKITATVGRSKTLVIFVNQTRMKIGTLPYMNPETT
                     SGGTALRFYSSVRIDVRRIGSIKEGEEVSGNRVRAKVVKNKVSPPFRDAEFDIMFGSG
                     ISQVGELIDMGSKMNIVEKSGTWFSYGGERLGQGRENARGFLKENPDIAEKLKSDILT
                     KTGVIKPEKPGE"
     gene            1371..2504
                     /locus_tag="VFJ67_05085"
     CDS             1371..2504
                     /locus_tag="VFJ67_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET7289795.1"
                     /translation="MGSVSIDDILRAAVRHGASDIHIGTGRHPILRIEGKLTPVKSAP
                     VLSSDDVRDMAHQMMNPAQRERFETLYEMDLAYSISGLSRFRVNVFQQRGTLSIAIRL
                     IPYNILSFSSLNLPPVMEKIASEERGLVIVTGTTGSGKSTTLAAIVDYINRNKARHII
                     TVEDPLEYMHEDITSYINQREISIDTLSFANAMRAALREDPDVILVGEMRDLETMEIC
                     LSAAETGHLVLTTLHTLDAQESINRMLTIFPPHQHNQVRYQLAQVLKAIISQRLVPTA
                     DGKARVPAAEILIATERIKDLISDPQKTWEIRQAIHEGSLHYGMQTFDQCLYNLYKSG
                     LITYDEAMSQATNRNDLAMRIEGITSGSGSLIREETFGRQTSK"
     gene            2604..3323
                     /gene="tsaB"
                     /locus_tag="VFJ67_05090"
     CDS             2604..3323
                     /gene="tsaB"
                     /locus_tag="VFJ67_05090"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7289796.1"
                     /translation="MMRVLAIETSTYSGSIAVSEGERIIGELYLDMGPSHSERLVPAI
                     DRMLSDLGIERKDLGGVAVSLGPGSFTALRVGISTAKGLSYSLGIPVTGASSLEILAM
                     NVPFSPLQVCAVTDARKGELFAALFRTDDGAVRRITEDAIVAPGKLAETIKEKTIFIG
                     EGALLYRDFLEDNESWGEAPVFCPAYMNYPRAAALAFYGFRRFAGGHADEVMGLAPMY
                     LRKPDAELTAKGSQHDGIGHN"
     gene            3301..3519
                     /locus_tag="VFJ67_05095"
     CDS             3301..3519
                     /locus_tag="VFJ67_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HET7289797.1"
                     /translation="MMESDIIEKLLEGDEEFKRIYFEHRELDDVVRSLESKGTLTLDD
                     EMEVRKLKKVKLSLKDRMEAKIAEWKNK"
BASE COUNT          940 a          946 c         1140 g          692 t
ORIGIN      
        1 agaatattct gggcgttccg aaagacactc tcgacacgta cggcgcggtg agcgcgcccg
       61 tcgtggaggc tatggctcat ggtgtgagga agctcgcggg ctcggacata ggcctggccg
      121 tatcgggcat cgcggggccc ggagggggca ctcccgagaa gccggtcggc acggttttca
      181 tagggatcga ttcgggcaag ggggggacgc gctcgaagaa attcctcttt cacgggacga
      241 gggaggagat aaagctcgtt acgtcctctc acgcgctccg tcttattatg caaatttttt
      301 taaataacgt ataatctgga gctactatgg caaaacaaac attctcacca gattcatcag
      361 aaaaagaaaa aacgatagag ctcgcgatat cttctataga gaagcagttc gggaagggct
      421 ccatcatgag gctcggggcg gaagcgccca taccccagct ctccgtaata tcctccggct
      481 cgctggggct ggacatagcg ctgggagtgg gcggataccc gagagggagg atagtcgaga
      541 tattcggccc cgaatcttcg ggaaagacga cgcttgcgct ccacgtaatc gcagaggccc
      601 acaagaaggg cggcatcgcc gcgttcgtcg acgccgagca cgcgctcgac ccgaactacg
      661 caaagaacct cggggtgaag atagacgacc tcctcatatc gcagcctgat ttcggggagc
      721 aggcgctcga gatagtggac acgctcgtaa ggagcggcgc ggtcgacgtc atcgtgctcg
      781 actcggtcgc tgcgctcgtg cccagggcgg aaatagaggg cgagatgggg gacgcgcacg
      841 tggggctcca ggcgaggctc atgtcccagg cgctccggaa gatcacggcc acggtaggca
      901 ggtcgaagac actcgtcata ttcgtcaacc agaccaggat gaagattggg actctcccct
      961 acatgaaccc cgagacgacg agcggcggga cagcgctcag gttctattcc tccgtgagga
     1021 tagacgtgag gcgcataggc tctataaagg aaggggagga ggtatcgggg aacagggtca
     1081 gggccaaggt cgtaaagaat aaggtatcgc cgcctttcag ggacgccgag ttcgacatta
     1141 tgttcggaag cggtatctcc caggtcgggg agctcataga catgggctcc aagatgaata
     1201 tagtcgagaa aagcggcaca tggttctcct acggggggga gaggctcggg caggggaggg
     1261 agaacgcgag gggtttcctc aaagaaaatc cggatatagc ggaaaaacta aagagcgata
     1321 tacttacgaa aacgggggtc ataaagccag agaaacccgg ggagtagtat atgggctcgg
     1381 tgagcatcga cgatatactc cgcgcagcgg tcaggcacgg cgcatcggat atacacatag
     1441 ggacgggcag acacccgata ctgcgtatcg aggggaagct cacaccggtc aagagcgcgc
     1501 ccgtgctctc gtccgacgac gtgagggaca tggctcacca gatgatgaac cccgcccagc
     1561 gcgagaggtt cgagacccta tacgaaatgg acctcgccta cagcataagc gggctctccc
     1621 gtttcagggt caacgtattc cagcagagag ggaccctgtc gatagccatc aggttgatcc
     1681 cttacaacat actgagcttc agctctttga acctccctcc cgtgatggag aagatagcga
     1741 gcgaggagag agggctcgtt atagtcacag gcacgacggg gagcggtaag tccactacgc
     1801 tcgccgcgat agtcgattac atcaacagga acaaggcccg gcatatcata acggtcgaag
     1861 accctctcga gtacatgcac gaggacatta cgagctacat taaccagagg gagataagca
     1921 tagacacgct ctccttcgcg aacgccatga gggccgcgct cagggaagac ccggacgtca
     1981 tactcgtcgg cgaaatgaga gacctcgaaa cgatggagat atgcctgtcc gcggccgaga
     2041 ccggacacct cgtgctaacg acgctccata cgctcgacgc gcaggaatcg ataaaccgta
     2101 tgctcacgat attccctccc caccagcaca accaggtgag gtatcagctt gcgcaggtgc
     2161 tgaaggcgat aatatcgcag aggctggtcc cgacagccga cggaaaggcg cgggtgccag
     2221 cggccgagat actcatagca acggaaagga taaaggacct gatctccgac cctcagaaga
     2281 cgtgggagat aaggcaggcg atacacgagg ggagcctcca ttacgggatg cagaccttcg
     2341 accagtgtct ttacaacctc tacaagagcg gcctgatcac atacgacgaa gcgatgagcc
     2401 aggcgaccaa caggaacgac ctcgccatga ggatagaggg catcacttcg ggatcgggat
     2461 ctctcatacg ggaagagacc ttcggtcgtc agacatcgaa ataacaccgc ctttttcgct
     2521 cccgaacatt cgcgcggaca gttagccgct tctttcggtt cccggggcaa tccgttataa
     2581 tttgatatag tcaaatagaa ggtatgatga gagttctcgc aatcgaaact tcgacgtatt
     2641 cgggcagcat cgctgtctcg gaaggtgaac gaataatcgg agagttatat ctcgacatgg
     2701 gcccttctca ttcggagagg ctcgtgccgg cgatagacag gatgctttcc gatctcggga
     2761 ttgagagaaa ggacttgggc ggggtagccg tctccctggg gccgggctcg ttcacggcgc
     2821 tgagggtggg catatcgacg gccaagggcc tttcgtactc cctcggcatt cccgttacag
     2881 gggcatcgtc attggaaatt ctcgcaatga acgtgccctt ttcgcccctt caggtctgcg
     2941 ccgttacgga cgcgagaaag ggggagctct tcgcggccct cttcaggacg gacgacggcg
     3001 ccgtcaggag gatcaccgaa gacgctatag tcgcgcccgg caagcttgcg gaaacgataa
     3061 aggaaaaaac tatatttata ggcgaaggcg ctttacttta tagagatttt ctggaagata
     3121 atgagtcatg gggggaagct ccggtgtttt gtcccgctta tatgaactac ccgagggctg
     3181 cagccctcgc gttttatggt ttcagaaggt ttgccggagg gcatgcggat gaggtgatgg
     3241 gtctcgcgcc catgtattta cgaaaaccag acgcagaatt aaccgcaaag gggagccaac
     3301 atgatggaat cggacataat tgaaaagctg ctcgaagggg acgaggagtt taagcgcatc
     3361 tacttcgagc acagggagct cgacgatgtc gtaaggagcc tcgagagcaa aggcactctc
     3421 acgctcgacg acgagatgga agtcaggaag ctgaaaaaag taaagctttc gctcaaggac
     3481 agaatggaag cgaagatcgc tgaatggaag aataaataag aactgcgcgc cgctgtgtga
     3541 ccttaagcgc cgcgcaaaaa atacgggggt aattttcaaa tggcgagaat gatcggtgcc
     3601 gagatgtttt tcgagactct tatacacgag ggcatagacg tggtgttcgg tctgccgggg
     3661 gggtacgtgc tcaaggtgta tgacgtgatg ccgaagtact cggaccgcct caggcacg
//
LOCUS       DASSJH010000237         6740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1136057, whole genome shotgun sequence.
ACCESSION   DASSJH010000237 DASSJH010000000
VERSION     DASSJH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136057"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFJ67_05100"
     CDS             <1..1059
                     /locus_tag="VFJ67_05100"
                     /inference="COORDINATES: protein motif:HMM:NF025499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HET7289798.1"
                     /translation="NYASLPKGMIMFHKYPVRNRTAFEEHLAEALAYARDGSGRARVH
                     FTLSPEHLGIVSAFVGGVKGLYEREGGSLDVTYSVQKPSTDTIAVDMENRPFIGDDGK
                     PVFRPAGHGALIENLNALGCDIAFIKNVDNVVPDRLREETCLWKKALGGYLLALESLI
                     FGALDKLEKGGGYRDVAEELSRLSNLGLEIPVSEHTGAGDAQARLVSALGRPLRVCGV
                     VKNQGEPGGGPFWVRGKGGGLTKQIVESAQVDMSDPGQKRVWESSTHFNPVDLVCGLK
                     DRKGTPYDLTRFIDSDAGFISVKSKDGRALKALELPGLWNGAMAFWNTVFVEVPVITF
                     NPVKTVFDLLKPEHQPKL"
     gene            1147..1479
                     /locus_tag="VFJ67_05105"
     CDS             1147..1479
                     /locus_tag="VFJ67_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET7289799.1"
                     /translation="MSEKRYGKEVVYVDPDFMDLIPFFLESRHKEIVTIRECIANADL
                     KEAQRLGHGMKGAGGGYGFQEISSIGKSIEEAAKAGNTGEIEDALERLAEYLAVVEVL
                     PGKSEMLD"
     gene            1539..2846
                     /locus_tag="VFJ67_05110"
     CDS             1539..2846
                     /locus_tag="VFJ67_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289800.1"
                     /translation="MANARKTVTEDVRVKLPRDLTDAAAAYKEALGILDSLYARQLSN
                     ELTHSLEKDIWNEIAETLKKGADGLSGGARMSYEQGSASLARGIFDLFPLDAGSSNGK
                     DAIEIILGRVFSPEGDGESYYAGAAGRENREYDGLISEYDGNMGLFTDALMVSRGYKG
                     KVFQGDLPHIKCLDVFSLSGGLNARHKPICVFFSGGTKENVSSLSRMTVFINLYAARF
                     KALTEKIALKYLAGAEALEDMTPGETARLLLIWLRGHDAGHFIGADRLGRVMSEFDSN
                     YMVLHELKSDMIALYNMKHLSGSLLDGGLLHKAYLLTIAEMLRYMRREGFVKHPDTGS
                     AYLAYRHFRDMGAIRHDPWGGKFIVDMDRLEKVVDEFAWDLVRLFADGNEARARGFVN
                     SWGWLGVEDTDGLPRGCPEELRRMIADTEIPHEINYNFVTSFG"
     gene            complement(3222..4316)
                     /locus_tag="VFJ67_05115"
     CDS             complement(3222..4316)
                     /locus_tag="VFJ67_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289801.1"
                     /translation="MERWSAFLVASALFVLLTLSSSTGIAQSPRRIQLESTRGFPSIE
                     HTDWLGDPNTLFVAPSQSVTSTIRVITFTPPYQYTGRVRLSATCCYIPDTLIWVGTGV
                     EVYVGATLTAATLPRLPLDSQNPPGQQIIPPYIYVDVPGQSVEATLYVTAGPNVTPGN
                     WEARIAAENPNQGVNEYIRVMINVLPAWPPDGAAPACMPGLEVISLNSIKPPPYTWKA
                     NNLGNTSYYLGIKSATLPIGMDVTIKKAPVFPRLEPTEAIITFKNTRGWPVGIRKSDS
                     RSCAVTNPTTTVYEGQTATIRINRTDTTTLVFSKSTCRFWFIGCWSVGMDDIFQFSEG
                     PFWTLFGGKQADIETVKDWGKVWTDVYGSY"
     gene            complement(4493..6589)
                     /locus_tag="VFJ67_05120"
     CDS             complement(4493..6589)
                     /locus_tag="VFJ67_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289802.1"
                     /translation="MTRHGRDGDFMAMEAVFKDDFSEFINDTGIQIRQRCGEKPALRD
                     IYVFPDLEILSPGNLAGAGPGAGRINSSALIGVSVSGGGVLVCGPEDSGKTSLLKVLA
                     HSLAEAGFFPVYVDGKNLSIPRPEETREFFLGKYGSQYTAQSRGLITAEPREKWILLL
                     DNADRIRTGSAELGSFLLGLNDHFGGSILTAVNPPEFADLPKGLSVCGVPAGYAVCGI
                     AELGQTLREELISKWTHAGGAVDEEGAARRKDKLRGAVDKVLARNLVPSYPVFILTIL
                     QMADSEDLEGPGASSYGQYYDYLITKSLKEVVRREDLPFFMSLLSGLAYRMFSRRSRW
                     ISEEDIEALIGDVSADFPSHPEARGPVITALLGSETLAGRESGYEFRYGYIYHYFAAL
                     YFSRNLQNENVKEEVRRLSGSLDDEEYANIMLFLIHLSGDPFLLEQVARNVEYAAAAE
                     EPYTYEEEEQYLDELISELEKLIDKDRKLREIRAEFLPSRDMPERQPIGFRTNGTDIR
                     VTRRKEAALHGSARKSQFVDALLRISCELLKGQPTGGSYYIPGEFDENVYALWLSSLR
                     RRKKEADRMREAAEGMEERIFKGGGPEEDFSYLTALYGLYMRLYKRGVRQLSLLTEAG
                     SPEYENAIPDELMRICSGLFRKDQAPGIDTIGKFLEKNRERKFARELLRECVSLRAYL
                     FGEDEYSKEIAKLFNP"
BASE COUNT         1511 a         1844 c         1906 g         1479 t
ORIGIN      
        1 aattacgcga gcctgcccaa gggcatgatc atgttccaca aatatcctgt ccgtaacagg
       61 accgcattcg aggagcacct cgccgaagcc ctcgcttacg ccagagacgg aagcggcagg
      121 gcgcgcgtgc atttcacgct ctcgcccgaa cacttaggca tagtgagcgc gtttgtcggc
      181 ggcgtgaagg ggctatacga gcgcgaaggg ggaagcctcg atgtcaccta ctcggtgcag
      241 aaaccgtcca cggacactat tgcggtcgac atggagaaca ggcccttcat cggcgacgac
      301 gggaagccag tattcaggcc ggccggacac ggggcgctca tcgaaaacct gaacgcgctc
      361 ggatgcgata tagcgttcat aaagaacgta gacaacgtcg tgcctgacag gctcagggaa
      421 gagacctgtc tctggaaaaa ggcgttgggc ggttacctgc ttgcgctcga aagccttata
      481 ttcggggcgc tcgataagct ggaaaaaggc ggaggataca gggacgtcgc cgaagagctg
      541 tcccggctct cgaatctggg gctcgaaata ccggtctctg agcataccgg agccggagac
      601 gcgcaggccc gcctggtgtc ggcactaggc aggccgctca gggtctgcgg ggtagtgaag
      661 aaccagggcg agcccggcgg ggggccgttc tgggtgagag ggaagggcgg cgggctcacg
      721 aagcagatag tggaatcggc gcaggtggac atgagcgatc ccgggcagaa gagggtttgg
      781 gagtcctcga cacacttcaa ccccgtcgat ttagtctgcg gacttaagga ccggaaggga
      841 accccctacg acctcacacg gttcatcgac agtgacgcgg ggttcatatc cgttaaatcg
      901 aaagacggga gagccttgaa ggctctcgag ctgcccggac tctggaacgg cgcaatggcc
      961 ttctggaaca cggtgttcgt cgaggtcccg gtaatcacgt tcaacccggt caagaccgtt
     1021 ttcgacctgc tcaagcccga gcaccaaccc aagctctgaa cgcagatatt cggttccccc
     1081 gtttacccgg agagcgggat agggtgtatt attacctgag aagctcagcg ggaggcatgc
     1141 tcgcatatgt ccgaaaaacg ttacgggaaa gaagttgttt acgtggaccc cgatttcatg
     1201 gacctcatac ctttcttcct cgaaagcagg cataaggaga tcgtgaccat aagggagtgc
     1261 atagcgaacg cggacctcaa ggaggcccag aggctcgggc acggcatgaa gggcgcgggc
     1321 gggggatacg gtttccagga gataagcagc atagggaaga gcatagagga agcggcgaag
     1381 gccggcaaca cgggagagat cgaagatgct ctcgagagac tggcggaata cctggccgtg
     1441 gtcgaagtgc tgcccggaaa gagcgaaatg ctcgactgac gcccgcatag caaaaacccg
     1501 cttaaatccg tccgagttac ctgttaaaat accgggtcat ggcaaacgcc aggaaaaccg
     1561 taaccgaaga cgtacgcgtg aagctgccac gggacctgac tgacgctgcg gccgcttata
     1621 aggaggccct cggcatactc gacagcctct atgcgaggca gctctctaat gagctcactc
     1681 attcactcga aaaggatatc tggaacgaaa tcgccgaaac actgaaaaaa ggtgccgacg
     1741 gactctcggg cggagcgcgc atgagctacg agcagggctc ggcctcgctc gcgagaggca
     1801 tattcgatct ctttcccctc gacgccggaa gctcgaacgg aaaggacgcg atagagataa
     1861 tacttggcag ggtattctcg cccgaagggg acggcgagag ctattatgcc ggagccgccg
     1921 gacgagagaa ccgcgaatac gatgggctta tcagcgagta cgacggcaac atgggtctct
     1981 tcaccgatgc gctcatggtg tcgcggggat ataaggggaa ggtatttcag ggcgacctcc
     2041 cgcatatcaa gtgcctggac gttttctccc tctcgggcgg tctcaacgcc cggcacaagc
     2101 cgatatgcgt attcttctcc gggggcacga aggaaaacgt ttcctccctt tcgcgcatga
     2161 ctgtattcat aaacctctac gcggccaggt tcaaggccct taccgagaag atcgcgctta
     2221 aatatctcgc cggagccgag gcgctcgagg acatgacgcc cggggagacg gcgcggcttc
     2281 tcctcatctg gctcaggggt cacgacgccg ggcatttcat cggcgccgac aggctcggca
     2341 gggtgatgtc cgagttcgac agcaactaca tggtgctcca cgagcttaaa tccgacatga
     2401 tcgctctcta caacatgaag cacctctcgg ggagtctcct cgacggaggt ctcctacata
     2461 aggcatacct tctcacaatt gccgagatgc tgaggtacat gaggagggag gggttcgtca
     2521 agcacccgga cacgggctcg gcttacctcg catacaggca cttcagggac atgggggcga
     2581 tacgtcacga cccgtggggc gggaaattca tcgtggacat ggacaggctc gaaaaggtgg
     2641 tggacgagtt cgcatgggac ctcgtcaggc tcttcgccga cgggaacgag gcgagagccc
     2701 gggggttcgt caacagctgg gggtggctcg gggtcgagga cacggacggg cttccgaggg
     2761 gctgcccgga agagctccgg aggatgatcg ccgacacgga gataccgcac gagatcaatt
     2821 acaacttcgt gacgagcttc gggtgaggtg caaagccggt atctctcaaa cgtaaagctc
     2881 ccctatcaca atccgccgag ggccgaacga ttgaagtatt ttttctaagg cagacaaatg
     2941 actgcatcca tgcagctggc cgcctgtgta aagaaaatcc catatggacg ggttgtgagc
     3001 gtgcagtcga acgattggga gcaggaattg acgcagacat caggcaaaat gattctcctg
     3061 tccagcaggc actggccttt catggcagcc ggcgtagtgg cgacgtcaac cacgaccagc
     3121 cctacaatga taataacggc taatatgaat aataatccga tacttttcat gacttggctc
     3181 cttggaactt gggatcagag gctttgactt aattaaagtt actaataaga tccatacaca
     3241 tcggtccaaa ccttccccca atcctttacc gtctctatat ctgcttgttt tcctccaaat
     3301 agcgtccaga agggcccttc gctgaattga aagatatcgt ccatcccgac gctccagcac
     3361 cctataaacc agaatctgca agttgatttg ctaaagacga gcgttgttgt atcagtcctg
     3421 ttaattctga ttgttgcagt ttggccttca tatactgtag ttgtcggatt tgtgacagcg
     3481 caacttctgc tgtccgattt ccttatcccc acaggccatc ccctggtatt cttgaaagtt
     3541 ataatagctt cagtgggttc tagtcttgga aaaacaggtg cttttttaat agtaacatcc
     3601 atgccgatcg gcagagtcgc tgattttatt cccagataat acgacgtatt tcccaagttg
     3661 ttcgctttcc acgtataagg cggtggtttt atagagttca gtgatatgac ctccaggccg
     3721 ggcatgcacg ccggcgcagc gccgtcgggc ggccaggccg gaagaacgtt tatcattacc
     3781 ctgatgtatt cgttcacccc ctgattcggg ttctcggcgg cgatacgagc ctcccagttg
     3841 ccgggtgtta cgttgggacc ggcagtcacg tacaaagtcg cttcaacaga ttgcccgggg
     3901 acatccacgt atatatacgg gggaattatc tgttgtcccg gtgggttctg ggaatctaga
     3961 ggcagccgcg ggagcgtcgc ggcagtcagc gttgcaccga catatacttc aactccggtt
     4021 cccacccata tgagggtgtc cgggatataa cagcaagtag cgctcagcct cactctgccg
     4081 gtgtattgat agggcggcgt aaaagtgatt acccggatcg tggatgttac cgattgtgag
     4141 ggagcgacaa acaatgtgtt tgggtccccg agccagtcgg tatgctcaat tgagggaaat
     4201 ccccgggttg actcgagttg gatgcgccgc ggggactgtg caattcctgt agaggatgag
     4261 agcgtcaaaa gtacaaacag agcagaagcg acgagaaacg cggaccacct ttccatgacc
     4321 cctccctcgc ggtatcctcg tcatgcttga aaacccctca gggcaattat gcagtcgaca
     4381 cgggagcgtc tggcttgccc atctgagaga gatgaggaaa ccacatcttg ccttatattc
     4441 cgagactaca atgaatctga gctgatgtca attgtggatt aatccgccgt atttacgggt
     4501 tgaagagctt cgcgatctcc ttcgaatact cgtcctcccc gaacagataa gcgcggagtg
     4561 aaacacattc cctgaggagc tccctcgcga attttctctc tctattcttc tcgagaaatt
     4621 tgccgatagt atcaatgcct ggagcctggt ctttacggaa gaggcccgag catatcctca
     4681 tcagctcgtc agggatcgcg ttctcgtatt cgggagagcc cgcttccgtg aggagtgaca
     4741 gctgcctcac gccgcgtttg tataacctca tgtagagtcc gtaaagcgcg gtaaggtacg
     4801 agaaatcctc ttccggtccg ccgcctttga atatgcgctc ctccattccc tcggccgctt
     4861 ccctcatcct gtcggcttct ttttttcttc tcctcaggga cgacagccac agggcataaa
     4921 cgttttcgtc gaattcgccc ggtatataat aggagccgcc ggtcggctga cccttcagca
     4981 attcgcatga gattctaagg agagcgtcta cgaattgcga cttccgcgcc gaaccgtgaa
     5041 gggcggcctc cttccttctc gttaccctta tgtccgtgcc gttcgtccgg aacccgatgg
     5101 gctgcctctc gggcatgtcc cgggacggca gaaactcggc ccttatctcc ctcagtttcc
     5161 tgtccttgtc tatcagtttc tcgagctcgc ttatgagctc gtccaggtat tgctcctctt
     5221 cttcgtacgt gtaaggctcc tcggcggccg cggcgtactc gacgttgcgg gctacctgct
     5281 cgagcaggaa aggatccccc gaaaggtgga tgaggaacag cattatgttg gcgtactctt
     5341 catcgtcaag gcttccggat agtcttctca cttcttcctt cacgttttca ttctggaggt
     5401 tccgggaaaa gtagagcgcg gcgaagtagt ggtatatata tccgtacctg aattcgtaac
     5461 ccgattccct gcccgcgagg gtctccgatc cgaggagcgc ggttattacg gggccccgag
     5521 cttccgggtg actcgggaaa tccgccgaga catcgccgat cagggcttcg atgtcttcct
     5581 ccgatatcca cctgctcctc cttgagaaca tcctgtatgc gagcccggac aggaggctca
     5641 tgaaaaatgg cagatcttcc ctgcgtacga cctccttgag tgatttggtt atcaggtaat
     5701 cgtagtactg cccgtaggag ctcgcgcccg gcccctccag gtcttcggaa tcggccattt
     5761 gcagtattgt caatataaat accgggtagg aagggacgag attcctcgcg agaaccttat
     5821 ccaccgcgcc tcttagtttg tcttttctcc gagcggctcc ctcttcgtcc accgcccctc
     5881 ctgcgtgcgt ccatttcgag atgagctcct ctctgagcgt ctgtccgagc tccgctatcc
     5941 cgcaaacggc atagccggcg ggaaccccgc aaactgacaa tccctttggc aggtcggcga
     6001 attcgggcgg gttcacagcg gtaagaattg acccgccgaa atgatcattg agaccgagca
     6061 ggaaagatcc cagctcggcc gacccggtcc ttatcctgtc ggcgttgtcg aggaggagta
     6121 tccacttctc ccggggctcg gccgttataa gaccccggct ttgagcggtg tattgcgaac
     6181 cgtatttccc gaggaagaat tcgcgcgttt cttcggggcg cgggatgctg agattcttcc
     6241 cgtcaacata aacggggaag aagcccgctt cggcgagact gtgggcaagg accttgagca
     6301 gtgaggtctt tcccgaatcc tccgggccgc atacgagcac accgcctcct gagaccgaga
     6361 cgccgatgag cgccgatgag tttattcttc cggcgccggg ccccgcgccc gccaggtttc
     6421 cggggctcaa tatctcgaga tcggggaata cgtatatgtc ccggagagcg ggtttttccc
     6481 cgcatctctg tcttatctgg atgccggtat cgttaataaa ttcgctgaaa tcatctttga
     6541 atacagcttc cattgccatg aaatccccgt ctcttccgtg gcgcgtcaac cccgttatcc
     6601 ggccggcggg aaacacaggc gcgcattttt tctcaataaa ttatttagat tatacaggga
     6661 ggtctgaagg aggagcaaat atttgttgtg gttccggata gtttaattta aaatccaatg
     6721 aaatcataaa taatcgttcg
//
LOCUS       DASSJH010000238         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_491244, whole genome shotgun sequence.
ACCESSION   DASSJH010000238 DASSJH010000000
VERSION     DASSJH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491244"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VFJ67_05125"
     CDS             <1..1428
                     /locus_tag="VFJ67_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET7289803.1"
                     /translation="NDLYHYAFSENAVTSEKEEPAKEDGFVTGEESRERLGVKAFLRD
                     SAIMYTGLWASRWFYVRNKNSRIFSASFSGWWHNITQWPVWDDGDSFFTNWVTHPIIG
                     SQEYLFYRAMGHSYLVSALGVVLQSTLFEYTIEGTVEVPSLVDLVSTPLVGVPMGFAL
                     EKSSDWLISTDFVPAKILGHILNPMRNFIHDRQVGIYDPFSKTFMSVSGPIVFTPNKS
                     IAIDLAYPYFMEEPIPLGRFRADLEVVNLKKKEDGQFIFYSLRADVPSKNGLWGAYVR
                     IAQSGVNSVMINGEEVRDGFEFANVLVGGKLLLWKSHNSAVAGGMELVLPTSYKDNID
                     RLSTVTLFRRNFPINLQKAWTVTPYVTAAAWKGIFNVQAMASTDVVLNAGELEGNSAE
                     WRLNYGASAGANFPVIASPVLYAEFNAYTCLTADTFQKNDMFMTGGVRFGRKFSPGFA
                     MQFPVYGADKDIDRYSYMFDFQVRF"
     gene            1586..>2208
                     /locus_tag="VFJ67_05130"
     CDS             1586..>2208
                     /locus_tag="VFJ67_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7289804.1"
                     /translation="MKTRVLVLGAGFGGLELSTLLSEAFGDEVDVTLIDKSDHFIFGY
                     SKLDVMFDRAEPDAVRLPYRNFVKPGVRFLQQTVTAIDPDARRVTTDAGTYESDFLVV
                     ALGADYDLDATPGLADVGSEFYSVAGAERLRGALQNFSKGPAVIGVSAAPFKCPPAPS
                     ECALMLHDFLTEKGVRSRCEITLVLPLGSPVPPSPDTSRALLSAFSER"
BASE COUNT          455 a          641 c          660 g          452 t
ORIGIN      
        1 aacgaccttt accactacgc cttttccgag aacgctgtca cctcggagaa ggaagagccc
       61 gcgaaggaag acggattcgt cacgggcgag gagagccggg agcgcctggg cgtaaaagcc
      121 tttctccgag actcggcgat aatgtacacg ggactctggg cgtccaggtg gttctacgta
      181 aggaacaaga acagcaggat attcagcgcc tcgttctcgg gctggtggca caatataacg
      241 cagtggcccg tatgggacga cggcgattcg ttcttcacca actgggtcac gcacccgatc
      301 ataggctcac aggagtacct cttctacagg gccatgggtc acagctacct cgtctccgcg
      361 ctgggcgttg tgctccagag cacgctgttc gagtacacga tagagggcac tgtcgaggtc
      421 ccttccctcg tcgacctcgt ctcgacgccc ctcgtaggcg tgccgatggg gttcgcgctc
      481 gagaagtcat ccgactggct catatccaca gacttcgtcc cggccaagat actcggccac
      541 atactcaacc cgatgaggaa cttcattcac gacaggcagg tcggcatata cgacccgttc
      601 tcgaagacct tcatgtccgt gagcgggccg atagtgttca cgcccaataa aagcatagcg
      661 atagacctcg cgtacccgta cttcatggag gagcccatac cgctcggcag gttcagggcc
      721 gacctcgagg tagtcaacct caagaagaaa gaggacgggc agttcatttt ctattccctc
      781 cgcgccgacg tgccttccaa aaacgggctc tggggcgcgt acgttaggat agcgcagtcg
      841 ggggtgaact cggtcatgat aaacggcgag gaagtgaggg acgggttcga gttcgcgaac
      901 gtgctggttg gcggaaagct cctcctctgg aagtcccaca actcggccgt ggcgggcggg
      961 atggagctcg tgctcccgac ctcgtataag gacaacatag acaggctctc tacggtcact
     1021 ttattcagaa ggaacttccc cataaacctc cagaaggcgt ggacggtgac gccctacgtg
     1081 actgcggccg cgtggaaggg tatattcaac gtgcaggcta tggcttcgac cgacgtcgtg
     1141 cttaacgcgg gcgagctcga ggggaactcg gccgagtgga ggctcaatta cggagcctcg
     1201 gcaggggcta atttcccggt catagcgtcc ccggtgctat acgctgagtt taacgcctac
     1261 acctgcctta cagcggacac gttccagaag aacgacatgt tcatgaccgg gggagtaagg
     1321 ttcgggagga agttcagccc cggcttcgcc atgcagttcc ctgtttacgg ggcggacaag
     1381 gacatagaca ggtacagcta catgttcgac ttccaggtga ggttctagtt ttaaataatt
     1441 tgtcattgcg agctgcgaag cagcgtggca atctcatcat ttcttttaat tccctctcct
     1501 taaatcgcgc tggacaaaag aggagcggtg catcaacata ttacgtttac gtataaattc
     1561 actcacaccg ccggaggtca tttcaatgaa gacgcgagtc ctggttcttg gagcgggttt
     1621 cggaggcctc gagctcagca ccctgttgtc ggaggcgttc ggcgatgagg tcgatgtgac
     1681 cctcatcgac aagagcgacc acttcatctt cggctactcg aagctcgacg tgatgttcga
     1741 ccgggcggag cctgacgcgg tccgcctgcc ctaccgcaac ttcgttaaac cgggcgtgcg
     1801 ctttctacag cagacggtta ccgcgatcga cccggacgcg cggcgcgtca cgaccgatgc
     1861 cggcacctac gagtccgatt tcctggtcgt cgcgttaggc gccgactacg acctcgacgc
     1921 cacgccgggt ctcgccgatg tgggaagcga gttttactcc gtagccgggg ccgagcggct
     1981 gcgcggggcg cttcagaatt tctcgaaagg cccggccgtg atcggcgttt ccgcggcgcc
     2041 gttcaagtgc ccgcccgcgc ccagcgaatg tgcgcttatg ctgcacgatt tcctgacgga
     2101 aaagggcgtc cgcagccggt gcgaaattac attggtgctg ccgctgggga gcccggtgcc
     2161 gccgtcgccc gatacttcga gagcgctgct gagcgccttt tcggagcg
//
LOCUS       DASSJH010000239         5627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_594609, whole genome shotgun sequence.
ACCESSION   DASSJH010000239 DASSJH010000000
VERSION     DASSJH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594609"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VFJ67_05135"
     CDS             complement(<1..1116)
                     /locus_tag="VFJ67_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7289805.1"
                     /translation="MSHVITNIVYLISALLFILGLKGLNSPETARKGQKMAAIGMAMA
                     IIGTLFQHEVITYEWIIVGMIIGSLAGIGIAAWTPMTKMPERTALSHAFGALAAALVG
                     VAEYYHGQDLNTVRMTAIGSEVMLGSLTFTGSMMAFGKLQGILPGSPWTYKGQNFFNI
                     SLLGVIVLIVIILVFFPATSFLFYIGLILALTFGVMLILPIGAADMPTVISILNSYAG
                     ISAALLGFALNNKILIIAGALDGASGFILSIIMCRAMNRSFTNVLFGAFGKREEADAT
                     PVDEDVSPKQARSGTPREAAEIFKTAKSVIVVPGYGMAAAQAQHSVRELADILQAQGT
                     DVKYAIHPVAGRMPGHMNVLLAEASVPYDVLVDMDDIN"
     gene            complement(1113..1415)
                     /locus_tag="VFJ67_05140"
     CDS             complement(1113..1415)
                     /locus_tag="VFJ67_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7289806.1"
                     /translation="MDGALLVAGLYIFMLAAFLGFLVISRVPPLLHTPLMSATNAISA
                     IIVVAAILVAGSGHGGLVTVLGTIAVACAMCNVVGGFLITERMLKMFKKGDGKSEK"
     gene            complement(1432..2562)
                     /locus_tag="VFJ67_05145"
     CDS             complement(1432..2562)
                     /locus_tag="VFJ67_05145"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HET7289807.1"
                     /translation="MRVGIIKETLENERRIAAVPETVSKMTKAGTEVLIESGAGSGSF
                     ISDGEFEAVGASISSRDEILSSADVLLKVNKPTTAEIEKMKEGAIVISFLQPFSSPEE
                     IKKLAGRKVSALSMEMVPRITRAQRMDALSTMSTVAGYKVVLLAADASGRFFPMLSTA
                     AGTIPPCKAIIIGAGVAGLQAIATAKRLGAVVTAFDVRPAAGEQVKSLGAEFVSLDVP
                     HEQAEDTGGYAKELPPEFYQKEQEIIRKYSKDTDIIISTALIPGKKAPVLITEDMVKE
                     MKPGSVIVDMAVEFGGNCTLSEAGKEVVKHGVTIIGYSNIPAMMPVHASLLYAKNILA
                     FVNLISPDGKSATLDLEDDIIKGSLITHAGEIVHPAIKEAVK"
     gene            complement(2827..3156)
                     /locus_tag="VFJ67_05150"
     CDS             complement(2827..3156)
                     /locus_tag="VFJ67_05150"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET7289808.1"
                     /translation="MKRLYTPRDESELVFLKSMFEASRMPYYVQNENFGSLYTGSYMS
                     SLNAKTIMVPEEIFDDAKDLIRSVNEDAVFEDERGGGGEGGSGAGSLLDGVLEFLSLK
                     RKGRERD"
     gene            3512..3982
                     /locus_tag="VFJ67_05155"
     CDS             3512..3982
                     /locus_tag="VFJ67_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7289809.1"
                     /translation="MQTLESILSGHYCFKGLEKKYLDMIVARATHASYDEGDYIFHEG
                     DKGDKLYIIQKGIVALETRLAPDRDPITIQMRGEGDVLGWAWLFPPHLCHFDAKAVAP
                     TEAVVLDGTFIRTKCEEDHDLGYELMKRFASLIQQRLQAVRLQNPNMYVVKSKT"
     gene            complement(4023..4439)
                     /locus_tag="VFJ67_05160"
     CDS             complement(4023..4439)
                     /locus_tag="VFJ67_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET7289810.1"
                     /translation="MNHDELMELIIEHYKHPKNRGPLENARVVQKGGNPGCGDIITVY
                     LDADEQGVVSRLSFEGEGCMVSQAGTSLILEKAEGMTLSEIEEMDPGTVTEILGKRLV
                     TTRPGCTHLGLNTLKLAVRKLRTEQDGVPAGVTGRG"
     gene            complement(4499..4924)
                     /locus_tag="VFJ67_05165"
     CDS             complement(4499..4924)
                     /locus_tag="VFJ67_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HET7289811.1"
                     /translation="MTKIDEIIGQFQDADYQETLAILLDYSENLPPLPEEYRDARDAG
                     FNRVPECETPVFIFVDVKDGRVHIHADVPKESPTVRGFVSLIVDAFDDATPLDIESAP
                     VNLIHSLSLDRKLGTRRMFGLSAVYNRIRSEVRKADEVH"
     gene            complement(5028..>5627)
                     /locus_tag="VFJ67_05170"
     CDS             complement(5028..>5627)
                     /locus_tag="VFJ67_05170"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET7289812.1"
                     /translation="VVFYGDKNNWWACYAYWVFKLFGHEKALVMNGGRQKWVEEGRPL
                     TKEVPNYPKAAYKVSGVNESIRAFRDDVTKHLGTKKPLIDVRSPKEFTGELLHMEAYP
                     QEGALRGGHIPGAKNVPWATAVKEDGTFKSADDLKAIYEEGKGLKKNDDVIAYCRIGE
                     RSSHTWFVLTYLLGFDKVRNYDGSWTEWGNLVKAPIEKP"
BASE COUNT         1125 a         1669 c         1548 g         1285 t
ORIGIN      
        1 gttgatgtcg tccatgtcga cgagcacgtc gtagggcacg ctcgcctcgg ccagcagcac
       61 gttcatgtgc ccgggcatcc ttcccgcgac cgggtgtatg gcgtatttca cgtccgtccc
      121 ctgcgcctgg agaatgtcgg caagctccct cacgctgtgc tgcgcctgag cggccgccat
      181 tccgtacccg ggcacgacga tgaccgactt cgcggtcttg aatatctcgg ccgcctccct
      241 gggagtgccg gagcgggcct gtttggggct cacgtcctcg tcgaccggag tcgcgtccgc
      301 ctcctctctc tttccgaaag cgccgaagag cacgttcgtg aaggagcggt tcattgccct
      361 gcacatgatg atcgagagta tgaagcccga agccccgtcg agcgcgcccg cgatgatgag
      421 gatcttgttg ttgagcgcga agccgagtag cgctgcggag ataccggcgt aggagttcaa
      481 tatggaaata acggtcggca tgtcggccgc gcctatcggc agaatgagca tcacgccgaa
      541 cgtgagggcg aggatgaggc cgatgtaaaa cagaaacgat gtcgccggga agaataccaa
      601 tatgatcacg attagtacga ttacgcccaa gagggatatg ttgaaaaagt tctggccttt
      661 atacgtccag ggcgagcccg gcagtatgcc ctggagcttg ccgaacgcca tcatgctgcc
      721 cgtgaaggtc aggctcccga gcatgacttc cgagcctatg gccgtcatcc tcacggtgtt
      781 gaggtcctgc ccgtgataat actcggcgac gcccacgaga gccgcggcca gcgcgccgaa
      841 cgcgtgcgac agagcggttc gctcgggcat cttcgtcatc ggagtccacg cggcgatgcc
      901 tatacccgcg agagacccga ttatcatgcc gacgattatc cactcgtatg tgatgacctc
      961 gtgctgaaag agggtcccta tgattgccat cgccatgccg atcgcggcca tcttttgtcc
     1021 tttgcgcgcg gtctcgggcg agttcaggcc cttcagtccc aggatgaaaa ggagggccga
     1081 tatcagatat acgatgttgg ttataacgtg gctcattttt cgctcttacc gtcccctttt
     1141 ttgaacattt tcagcatgcg ctccgtgatg aggaaaccgc ccacgacgtt acacatggcg
     1201 caggcgaccg ctatcgtgcc gagcacggtt accaagcctc cgtgaccgct ccccgcgacg
     1261 agtatcgcgg ctacgacgat gatcgccgat atagcgttcg tggccgacat cagcggcgtg
     1321 tggagaagcg gcggaacccg cgatatgacg agaaacccga ggaatgcggc aagcatgaat
     1381 atatataatc cggcgactaa taaagctccg tccatgagac tcctccttta attatttcac
     1441 ggcctctttt atggccggat gcactatttc tcccgcgtgc gtgataagcg agccttttat
     1501 tatatcgtct tcgaggtcga gtgtggccga tttaccgtcg ggcgagatga ggttcacgaa
     1561 tgccagtatg ttcttcgcgt aaagaaggct cgcgtgaacg ggcatcatgg ccgggatgtt
     1621 cgagtatccg attatagtca cgccgtgttt tacgacttcc ttcccggctt ctgacagcgt
     1681 gcagttaccg ccgaattcca cagccatgtc cactatgacc gagcccggct tcatctcctt
     1741 gaccatgtct tcggttatga gcaccggggc cttcttgccg gggataagcg ctgtgctgat
     1801 gattatgtcc gtgtccttag agtacttgcg tatgatctcc tgctccttct ggtagaactc
     1861 aggagggagc tccttcgcgt atcctccagt gtcctccgcc tgctcgtgcg ggacgtcgag
     1921 cgatacgaac tctgcgccga ggctctttac ctgctcgcct gcggccggcc tcacgtcgaa
     1981 cgccgtcacg accgctccga gacgcttggc ggtcgcgatc gcctggagcc ctgccacgcc
     2041 cgcccctatg attatcgcct tgcacggggg tatagtgccc gccgcggtgg aaagcatggg
     2101 gaagaacctc cccgacgcgt cagccgccag gagcactacc ttgtaaccgg cgaccgtgct
     2161 catcgtggag agcgcgtcca tgcgctgcgc ccgcgtgatc ctcgggacca tctccatcga
     2221 gagcgcggat accttgcgcc cggccagctt ctttatctct tcggggctcg agaagggctg
     2281 gaggaacgat atgacgatag ccccttcctt catcttctct atttcagctg tggtgggttt
     2341 gttgaccttt agcagcacgt cggcgctcga aagtatctcg tcacgggagg atatactcgc
     2401 accgacggcc tcgaactcgc cgtcgcttat gaatgatccg gagccggccc cggattcgat
     2461 caggacctcc gtccccgcct tcgtcatctt gctcactgtc tcgggaacgg ccgcgatgcg
     2521 gcgctcgttt tccaatgtct cttttattat tcctacgcgc atgaattatc tccgccctcc
     2581 gcaaaccggg ggattagaaa ttacccaaac tctaatcctg cgtcaatatc gaatctacca
     2641 taaaaaaggc gcggaatgcc aacatgattt tcatttccac gaaatcaagc gctttatgat
     2701 tctattaata tttttagatc agtcaaggtt aggaggccta aacatcgaaa atcaggaggg
     2761 aactgtcacc ggggcccgaa ccgaaggaca ttatttataa aactggaacg ttccccgcat
     2821 gcgcggtcag tcccgctccc ggcccttcct ttttaaggag agaaactcga gcaccccgtc
     2881 gagaaggctc ccggcgccgc tgccgccctc ccctcccccg ccgcgctcgt cctcgaacac
     2941 ggcgtcctcg ttcacactgc gtatgaggtc cttcgcgtca tcgaatattt cctcggggac
     3001 cattatcgtc ttcgcgttga gagagctcat gtaggagccc gtgtagagag agccgaagtt
     3061 ctcgttctgg acgtaatagg gcatcctcga agcctcgaac atgcttttga ggaataccag
     3121 ctccgattcg tcccgtggcg tgtacagtct tttcataagc ggcaatcaat aatgtaccag
     3181 aattatttaa tactataatc aatagcccga ataaggcgca gggtttgatt gggggtgatt
     3241 actttacatt gtaggagcgg cttccagcag cgacaaacgg gggagcaaga cccgttcgtc
     3301 ctgagcttgt cgaaggattg aacgggcggg agattggaag cccaacccat tcatacttcg
     3361 atatacccaa tacgaacggg ttgggttgct ggcttgcacg gaagagccga atggaatcgt
     3421 atatgtgcac acattgtgtg ccgcaggttc tgcgcctcta aacatgatat aattatcccg
     3481 tacgagagta tatctccggg agaatgccgt tatgcagaca cttgaatcga tactcagcgg
     3541 gcactactgc tttaagggac ttgaaaaaaa gtatctggac atgatagtcg ccagggcgac
     3601 ccatgccagt tacgatgagg gcgattacat attccacgag ggcgacaagg gcgacaagct
     3661 ctatataatc cagaagggca tcgtagcgct cgagacgcgg ctcgcccccg acagggaccc
     3721 tataacgata cagatgagag gagagggtga cgtgctgggg tgggcatggc tcttcccgcc
     3781 gcatctctgc cacttcgacg ccaaggccgt cgcgcccacg gaggcggtcg tgctcgacgg
     3841 cactttcata cgcacgaagt gcgaggaaga ccacgatctg ggctacgagc tgatgaagag
     3901 gttcgcttcc ctgatacagc aaaggctcca ggcggtgagg ctccagaacc ccaatatgta
     3961 cgtcgtcaaa tccaagacct gaaaacaatc cggaatacta tttaatttaa ggaatacttg
     4021 cgtcagccgc gtccggttac gcctgccggg actccgtcct gctccgtcct cagcttcctc
     4081 acggcgagct tcagcgtgtt gagaccgagg tgcgtgcagc cgggcctggt ggtgacgagc
     4141 ctcttgccga gtatctccgt tacggtcccg gggtccatct cctctatctc ggagagcgtc
     4201 ataccctcgg ctttttcgag tatgagggac gtacccgcct ggctcaccat gcatccctcc
     4261 ccttcgaagc taaggcggct gacgacaccc tgctcatcgg catcgagata aactgtgata
     4321 atgtcgccgc acccggggtt cccgcccttc tggacgaccc gggcgttctc gagcggcccc
     4381 cggttcttcg ggtgcttgta gtgctcgatt atgagctcca tcaattcgtc gtggttcata
     4441 gggcgttctc tgtattagtg tagcggatgg acatgtccgg agacgtgcgc gggcgcggtc
     4501 agtgtacttc gtccgccttc ctgacctcgc tcctgatacg gttatacacg gccgagaggc
     4561 cgaacatgcg cctcgtgccg agcttacggt cgagactcag gctgtggatt aggttcaccg
     4621 gggcggactc tatatccagg ggagtcgcgt catcgaacgc gtcaacgatg agtgatacga
     4681 accccctcac agtcggcgac tccttgggca cgtccgcgtg gatatgcacc ctcccgtcct
     4741 tcacgtcgac gaatatgaag acaggggttt cgcactcggg cacgcggttg aaccccgcgt
     4801 ccctcgcgtc cctgtactcc tccgggagcg ggggcaggtt ctccgagtaa tcgaggagta
     4861 tggcaagcgt ctcctgatag tcggcgtcct gaaactgccc gattatttca tctatcttcg
     4921 tcatagccgt ctttaaacta ctcctgaaat agcaataaaa cgggatgcgg cggcagctgc
     4981 cggcacaccc cgcgttgtct ctcttattta ttatagtacc ttagcgttca gggcttctct
     5041 atcggagcct tgaccaggtt cccccactcc gtccacgagc cgtcgtagtt cctcaccttg
     5101 tcgaacccga ggagatatgt gaggacgaac cacgtgtggg aggacctctc ccctatcctg
     5161 caatacgcga tgacgtcgtc gttcttcttg agccctttgc cctcttcgta tatggccttt
     5221 agatcgtccg ccgacttgaa cgtcccgtct tcctttacag cagtcgccca gggtacgttc
     5281 ttcgcgcccg gtatgtggcc tcccctcagc gccccctcct ggggatacgc ctccatgtgg
     5341 agcagctccc ccgtgaactc cttgggcgag cgcacgtcta tgaggggctt cttcgtgccc
     5401 agatgcttcg taacgtcgtc cctgaaagcc ctgatggact cgttgacgcc ggagactttg
     5461 taagcggcct tcgggtaatt cgggacctct ttcgtcagag gcctcccctc ctcgacccat
     5521 ttctgcctcc cgccgttcat gacgagggct ttctcgtggc cgaagagctt gaacacccag
     5581 taagcgtagc acgcccacca gttgttcttg tcaccgtaga atacgac
//
LOCUS       DASSJH010000240         8702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1136818, whole genome shotgun sequence.
ACCESSION   DASSJH010000240 DASSJH010000000
VERSION     DASSJH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136818"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1250
                     /locus_tag="VFJ67_05175"
     CDS             51..1250
                     /locus_tag="VFJ67_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HET7289813.1"
                     /translation="MNPIQFLTEIIKEISIPGLFDIAFMALLIYAVLVLFNKTKARFV
                     LTGVIIISIVYLLARQFNLVLTTSVLQTFFAVIFIALIIIFQEEIRQFLEQIALWSLN
                     PQLALERSHGSSREEIEVLVSTVTDLAKERIGALIVLPGKTTIERAVEGGKTLNGKLS
                     EPLLKSLLDPHSIGHDGAIIIDKGNVVKFSCHLPLSKNFTMLLNRGTRHAAGLGLSEL
                     SDALCIIVSEEKGTVSVARNGELRQIGNEQLNLVLEKFYSEVNPIKRRRHWFTFFTKN
                     YREKAIAVLMAVALWFVFVHESKLVYMTYNIPVKYTTLPPDLKVEDIKPETVKVTFLG
                     PRRAFYFLNEKEVELFLKIPEARKGIKTVNISESSLKFPKDLSLEDIEPRRVLVRIDN
                     VSTKEAK"
     gene            1247..1393
                     /locus_tag="VFJ67_05180"
     CDS             1247..1393
                     /locus_tag="VFJ67_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289814.1"
                     /translation="MNIPEGGVSLDAENQGAVRHDPYDEAARAVLPPYQVSEYEPHYI
                     VEHD"
     gene            complement(1531..1902)
                     /locus_tag="VFJ67_05185"
     CDS             complement(1531..1902)
                     /locus_tag="VFJ67_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HET7289815.1"
                     /translation="MNKEKNVFGEELENCSSSPLTGFYRDGCCRTGPEDLGMHVVCTE
                     VTEEFLEFSKSRGNDLSTPIPEYEFPGLKPGDRWCICALRWKEAFDSGFAPPVVLAST
                     HESVLEVVSLEDLKKYAIDLA"
     gene            complement(2033..2596)
                     /locus_tag="VFJ67_05190"
     CDS             complement(2033..2596)
                     /locus_tag="VFJ67_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289816.1"
                     /translation="MVRKFFVVGKEGENKDLAPVPGEVYQDEAGLETGLIELLSELAL
                     LMEELKEIKDRDPVQTLKILSDSVNKVAVFAEHSLGGALREDFLRDALLEASGSFSHL
                     KLLHADRNRLSAQTAINLYAGWAGDAAGKNQAFRQISLGMVRVLESYLNFIAGFFGTP
                     HLAEEWNEALDIYISELKELVTSVVYR"
     gene            complement(2634..3983)
                     /locus_tag="VFJ67_05195"
     CDS             complement(2634..3983)
                     /locus_tag="VFJ67_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7289817.1"
                     /translation="MNLAKLLVREGMVSAAQVKESIESGTDAGYGVTESLVALGYVTE
                     RQIVEFLSREYGVPAMDIDGCEADESLLGLVPGETALEKFLVPISLEGADLTIAVSDP
                     SNILMLDDLGFITGKNIKPVIASERSIREKLSKYYGAGDAAVSTPDEDGSFDSGSAGG
                     PAVDSLHSSDINSPVEDIIRELEQYERELSSPPYPASGAEANSGADDSSGEDPTDESQ
                     PVQLHEVIGNGQTNQDNYVFDLTKEISPSSRLPLSLSEDVKDSPEPDEKETADVNVRN
                     EEEYLGESTAPSSGGYTIQEEKEEKPQSAPPVEDRKPPGPDMTVDGTGRTVLIVDVSP
                     TVRKIMTMTLERAGYRVYAAGDGMQALARINEAIPDLVFVDIKLPHMDGYQLCKVIKS
                     HGLTKSVPVILVSGKTGVLDKMKVRMAGASDLITKPFSAGALIEAAGKFAARGQLTQ"
     gene            4447..4785
                     /locus_tag="VFJ67_05200"
     CDS             4447..4785
                     /locus_tag="VFJ67_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET7289818.1"
                     /translation="MKKVEAIIKPFKLDDVKEALKEIGVQGLTVTEVKGFGRQKGHTE
                     LYRGAEYVIDFLPKIKLEIVVSDDMVTKVIDAIMDSAKTGKIGDGKIFIIPMEEVIRI
                     RTGERGEDAI"
     gene            4874..6292
                     /gene="glnA"
                     /locus_tag="VFJ67_05205"
     CDS             4874..6292
                     /gene="glnA"
                     /locus_tag="VFJ67_05205"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET7289819.1"
                     /translation="MPKKPSDVLKIIKDSSIEFIDLRFLDFVGMWQHFSVPAHVFELG
                     TFEEGLGFDGSSIRGWQSIHASDMLVIPDPESARIDSFMTHPTLAIICDIVDPITREP
                     YSRDPRNIAKKAAAYLKSTGIGDTAFFGPELEFFIFDDVRYDQTSNTGYYFVDSIEGI
                     WNSGRDENPNLGYKPRHKEGYFPTPPSDTFQDMRSEMVRELEKIGIEIEAHHHEVATA
                     GQCEIDMRFSPIVQMGDQMMWYKYVLRNVAKKFGRTVTFMPKPLFEDNGSGMHVHQSI
                     WKKDKPLFAGNGYAGMSDLAMNYIGGILKHARALCAFTNPTTNSYRRLVPGFEAPVNL
                     AYSARNRSAAIRIPMYSPSPKAKRLECRFPDPSCNGYLAFSAMLMAGLDGIEKKMSPG
                     EPLDKDIYALGPEELAGIPNVPGSLEDALKALEADHEFLMKGDVFTEDVIETWIEYKT
                     DKEVNPVKLRPVPYEFKLYFDV"
     gene            complement(6521..8497)
                     /gene="nuoL"
                     /locus_tag="VFJ67_05210"
     CDS             complement(6521..8497)
                     /gene="nuoL"
                     /locus_tag="VFJ67_05210"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET7289820.1"
                     /translation="MIVWIILIPLFGAAVNGLFFASGFWKKYLRGDEHTEKMVVSIIG
                     CGVVLVSALISTSLFFKLLSLDPARREIIQNLFEWIPSGSFTVSFSLLFDTLSCVMAL
                     IVTWVGFLIHVYSVGYMHHDEAYSKYFTYLNIFIFFMLTLVLGDSFPPMFVGWEGVGL
                     ASFLLIGFWYQNPENATAGRKAFVVNRIGDFGFILGIFLIFFVFGSVNYQEVFGKASD
                     PAFMSAVPAAVITVIALLLFVGAVGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTA
                     GAYMIARCNVLYSQSPTAQMVVVTVAALTSFLAATIAITQNDIKKVLAYSTISQLGYM
                     FMGIGVGAYTAGLFHLVTHAFFKALLFLGSGSVIHALSGEQDIRHMGGLRKFIPTTAI
                     TFLIGTLAIAGIPFFSGFFSKDAILGSVYERGYTIHWIIGMITVVLTAIYMGRLYLLT
                     FEGKSRVTDKVKDHIHESPATMTIPLMVLAFLSIVGGYMGVPEFMGELVGIHESNLFD
                     KFVSSSITMDNFISAGHHLFSHTNLIVLSISAALIGIAAACYFYYFKPSIPGRIASDK
                     TIEPLYEGSLNKWYVDELYNYVFVQPFKYLSEQASRFDLGIIDGAVNGVAAVLKDMAL
                     TLQAAQSGLLRNYATLMAIGAFAILVFIIVWRSF"
     gene            complement(8505..>8702)
                     /gene="nuoK"
                     /locus_tag="VFJ67_05215"
     CDS             complement(8505..>8702)
                     /gene="nuoK"
                     /locus_tag="VFJ67_05215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET7289821.1"
                     /translation="ELMINSANLAFVAFSRAHNDMTGQVFMIMSITVAAAEVAVGLAL
                     VVAVYAHRETLDVDILKILKG"
BASE COUNT         2202 a         2275 c         2377 g         1848 t
ORIGIN      
        1 gggatactca tctgatatac ttacaagacc aaacgaaagg gctagtttaa ttgaatccca
       61 tccaattctt aactgaaata ataaaagaaa taagcatccc cggcctgttt gacatagctt
      121 tcatggctct ccttatatat gcggtgctgg tgctgttcaa caagacgaaa gcccggttcg
      181 tgctgactgg ggtgataata atcagcatag tatatcttct ggcgcgccag ttcaacctgg
      241 tgttgacgac ctcggtgctt cagacgttct tcgccgttat attcatcgca ctcataataa
      301 tcttccagga ggagataagg cagttcctgg agcagatagc cctctggagc ctgaaccctc
      361 agctcgcgct cgagaggtcg cacggctcgt cgcgcgagga gatagaagtg cttgtgagca
      421 cagtcacgga cctggccaag gagaggatcg gcgcccttat agtgctcccg gggaagacaa
      481 cgatagagag ggcggtcgaa ggcggcaaaa cgctcaacgg caaattgagc gagcctctcc
      541 tgaagagcct gctcgacccg cactccatag gacacgacgg cgcgataata atagacaaag
      601 ggaacgtggt gaagttttcc tgccacctcc cgctctcgaa aaacttcacg atgctcctca
      661 acaggggcac ccgtcacgcg gccggcctgg ggttatccga gctgagcgac gcactctgca
      721 taatcgtatc tgaagagaag ggcacggtat ccgtcgccag gaacggggag cttagacaga
      781 taggcaatga gcagctgaac ctggtgctcg agaaattcta cagcgaagtc aatccgatca
      841 agagaagaag gcactggttt acgttcttca ccaagaacta cagggaaaaa gcgatcgccg
      901 tgctaatggc cgtcgcgctc tggttcgtat tcgtgcacga atcgaagctc gtgtacatga
      961 catacaacat ccccgtgaaa tatacgacgc tccctccgga cctaaaggtg gaggacatca
     1021 agcccgagac ggtgaaagtc acgttcctcg gcccgaggag ggcattttac ttcttaaacg
     1081 agaaggaggt cgagctcttc ctcaagatac cggaggcgag gaagggtata aagaccgtga
     1141 acatatccga atcgagcctg aagttcccga aggatttatc cctcgaggac atagagccca
     1201 gaagggttct ggtcaggatt gacaacgtct cgactaaaga ggcaaagtga atatacctga
     1261 gggcggagtg tctctcgatg ccgagaatca gggggctgtc cggcacgacc cttacgacga
     1321 ggccgcccgc gccgttttac cgccctatca ggtcagcgaa tacgagcctc actatatcgt
     1381 cgaacacgat tgaagccctc tcgcgaagta accagtccga ccgcccgctt aatttattca
     1441 ccttgaactg cgtagccgtt taacgcgccc ggcgcatatc aagaataata cagaacgaaa
     1501 cgggaaacac cccgcctcag gaagaggcgg tcacgccagg tctatagcat attttttgag
     1561 gtcttcgagt gaaacgacct ccagcacaga ctcgtgtgtg gacgcgagca cgacgggagg
     1621 agcgaacccg ctgtcgaacg cctccttcca ccttaacgcg catatgcacc acctgtcgcc
     1681 cggcttgaga cccgggaact cgtactccgg aatgggagtc gagaggtcgt tgccccggga
     1741 ttttgagaat tcgagaaact cctccgtgac ctccgtgcag acgacgtgca tgcccaaatc
     1801 ttcgggaccg gtcctgcagc agccgtcgcg gtaaaagccg gtcagtggtg atgacgagca
     1861 attctcaagt tcttcgccga aaacgttctt ttccttattc ataataaggc tccttcgagt
     1921 gcgagacgcc gaatgtgttt ttatggacag ggataatata acaaggctcc gggtgattgg
     1981 gaatccgggg atggaataaa agcggggatc ggcggtaaaa ccgtcggggc tgttacctgt
     2041 aaacgacgct cgtcacgagc tctttcagct cgcttatgta tatgtcgaga gcttcgttcc
     2101 actcctccgc cagatgggga gtgccgaaga atccggcgat gaaattcaaa taagactcaa
     2161 gcacacggac catgccgagg gatatctgcc ggaatgcctg gttcttgcct gcggcgtcgc
     2221 cggcccagcc ggcgtagagg ttgatcgccg tctgagcgga gaggcggtta cggtcggcat
     2281 gaaggagctt gagatgcgag aagctgccgg aggcctcgag gagagcgtcc ctcaggaagt
     2341 cttcccggag ggcaccgccg agggaatgtt cggcgaatac cgcgaccttg ttgacgctat
     2401 cggataggat tttgagggtc tggacagggt ccctgtcctt tatctccttt aattcctcca
     2461 ttaagagcgc aagctcggac aagagctcga tgagccccgt ttcaagcccg gcctcgtcct
     2521 gatacacctc tcccgggacc ggtgcgaggt ctttgttttc accttcctta ccgacaacga
     2581 aaaatttacg aaccatatct gcctatcccg aattcactcc tgcatacaat atatcattgt
     2641 gtcaattgac cgcgtgcggc aaatttccct gccgcttcga tgagagcccc cgccgagaac
     2701 ggctttgtta tcaaatcgct cgcacccgcc atcctgacct tcatcttatc gaggaccccg
     2761 gttttaccgg agaccagaat aacggggact gattttgtaa gaccgtggct cttgataacc
     2821 ttgcagagct ggtaaccgtc catgtggggg agcttgatat cgacgaacac gaggtcgggt
     2881 atcgcttcgt ttatcctggc aagcgcctgc atcccgtccc cggctgcgta tacgcgatac
     2941 cccgcccttt cgagagtcat tgtcattatc ttccttaccg taggacttac gtccacaata
     3001 agcacagtcc ttccagttcc gtccaccgtc atatcgggcc cgggcggctt ccggtcttct
     3061 acggggggag cggactgagg cttctcctcc ttctcctcct gaatcgtata accgccggaa
     3121 gacggggccg tcgattcacc gagatactct tcttcattcc tgacgttcac atccgctgtt
     3181 tctttttcgt ccggctccgg ggaatcttta acgtcctccg aaagagagag aggcaaccgt
     3241 gaggacgggc ttatttcttt agtaaggtcg aaaacgtaat tatcctgatt cgtttgtccg
     3301 ttcccgataa cctcatgtag ttgcacgggt tgagactcgt ccgtcggatc ctctcctgac
     3361 gaatcgtccg cgcctgaatt agcttccgca ccggaagcag gatagggagg ggatgaaagc
     3421 tctctctcat actgctcgag ctcccttatg atgtcttcta ccggactgtt tatatcggaa
     3481 gagtgaagcg aatccacagc cggaccgccc gcagagccgg aatcgaacga tccatcttca
     3541 tccggcgtag agaccgcggc atcgcctgct ccatagtatt tggacagctt ctctcttatc
     3601 gacctttcgg aagcgataac gggcttgata tttttaccgg tgatgaatcc caggtcgtcc
     3661 agcatcagga tattcgacgg gtccgagaca gctatcgtca gatcggcgcc ctcgaggctt
     3721 atagggacca gaaatttctc gagcgccgtt tcaccgggaa cgagtccgag cagagattca
     3781 tcggcctcac agccgtcgat gtccattgca gggactccgt actccctgga gaggaactcg
     3841 acgatctgac gttccgtcac gtaaccgaga gcgaccagcg attcggttac gccgtacccg
     3901 gcatccgtgc ccgattctat ggactccttt acctgtgcgg cgctgaccat accttccctg
     3961 accagcaatt ttgccagatt cattctcacg atatgaaaag tgagcaataa acatgccagt
     4021 gtgccgggaa agggatatga gacgtaacta gctgcataca ttgaaatata tagcagctaa
     4081 tcgcgtcttc cgcacacccc tgaactgaca aaaaacgtca aaagtatgaa atcaatgcgc
     4141 agtcatacag cccatgcacg atccggacgg aagactatct cagggagtac cgctgatact
     4201 ccgttagccc cgcatcaggg gagggagaga gctcatttcc acgaatgtcc gcccgccggc
     4261 aaatcaaatc acaggccggg gtttcatagc gccccgggga atgtttaatt tttagaatgc
     4321 tttgcttaag ttttaggcac tttttaagcg ggagttcaaa tataagcccc tattatcatt
     4381 gattgaaaag cttttagata agtggtatgt tgtttgcaat cattggatgg gaaggaggaa
     4441 tatcccatga aaaaagtaga agctatcatt aagcctttca agctggacga tgtgaaggaa
     4501 gcccttaagg aaataggtgt tcagggcctg acagtgaccg aagtcaaagg cttcggacga
     4561 cagaaaggac acaccgaact ctacagggga gccgaatacg tcatagattt cctgcccaaa
     4621 atcaagctcg agattgtcgt atccgatgac atggtgacaa aggtcatcga tgctataatg
     4681 gacagcgcaa aaacaggtaa gataggcgac ggcaagattt tcatcatacc gatggaagaa
     4741 gtcatcagga tcaggacggg cgagaggggc gaggacgcca tataaatttt gaattttaaa
     4801 caacaatagg aggaacagta agaatatgcc cacagcaata aagtctaaag gcaaaggagt
     4861 atcggaggat attatgccca agaagccctc tgatgtatta aaaattatta aagacagcag
     4921 tatcgagttc atagacctcc gttttctgga tttcgtgggg atgtggcagc atttcagcgt
     4981 tcccgcgcac gttttcgagc tcggcacctt cgaagaaggt ctcgggttcg acgggtcgag
     5041 catcagggga tggcagtcga tacacgcgag cgacatgctc gttatccccg accccgagtc
     5101 ggctaggatc gactcgttca tgacccatcc gacactggcg attatctgcg atatcgtaga
     5161 cccgatcacg cgcgagccct actcccgcga cccgcgcaac atagcgaaga aagctgcggc
     5221 ttatctcaaa agcaccggca tcggagacac cgcgttcttc ggaccggagt tagagttctt
     5281 tatcttcgac gacgtcaggt acgaccagac atccaacacc ggctattact tcgtcgattc
     5341 gatcgaggga atatggaact ccggcagaga cgagaacccg aacctcggct ataaaccgag
     5401 gcataaggaa ggatacttcc cgacgcctcc ctcggacacg ttccaggaca tgcgttcgga
     5461 aatggtcagg gagctggaga agatcggaat cgagatagaa gctcaccatc acgaagtcgc
     5521 cacggcggga cagtgcgaaa tcgacatgag gttctctccg atcgtgcaga tgggcgacca
     5581 gatgatgtgg tataagtacg tactcagaaa tgtggcgaag aagttcggaa ggactgtcac
     5641 cttcatgccg aagccccttt tcgaagacaa cggctccggt atgcacgtcc accagtcgat
     5701 atggaagaag gacaagccgc tcttcgccgg caacggatat gcgggtatga gcgacctcgc
     5761 gatgaactac ataggcggta tcctgaagca cgcgagagcc ttatgcgcat tcacgaatcc
     5821 gacaacgaac tcatacagaa ggctcgtccc gggattcgag gctccggtaa acctggcgta
     5881 ttcggcaagg aacaggagcg cagctataag gatcccgatg tattccccga gcccgaaggc
     5941 gaagaggctc gaatgcagat tcccggaccc gtcgtgcaac ggttacctcg cattctcagc
     6001 gatgctcatg gcgggactcg acggcataga gaagaaaatg agccccggag agccgctcga
     6061 taaggacata tacgcgctcg gaccggagga gctggccgga ataccgaacg taccgggatc
     6121 gctcgaagac gctctcaagg cgctcgaagc ggaccacgaa ttcctgatga aaggcgacgt
     6181 gttcaccgag gacgtcatcg agacgtggat cgaatacaag accgataagg aagtaaaccc
     6241 cgtgaagctg agacccgtgc cctacgaatt caagctttac ttcgacgtgt aagaaagccg
     6301 ccggcaaggc gaaataaaaa gatacgaaag cggcggccct tcactggacc gccgcttttt
     6361 ttatgcccga agcaaagtat taatcccctt aatcccaaaa ccaaaccgtg atctccagag
     6421 gtcgtttttc ggatgggccg gttcagcacg cggaattatt ttgtgagaat atccacgaaa
     6481 ggaaggtggt tcggaggaat gtcccgaacg acatgcccgt ctagaagctc cgccagacga
     6541 taatgaatac gaggatcgcg aaggccccta tagccatgag agtcgcgtag ttgcggagaa
     6601 gcccgctctg cgcagcctgg agcgtgagcg ccatgtcctt gagcaccgcg gcgacgccgt
     6661 tcacggcgcc gtctatgatg ccgagatcaa atctcgaggc ctgctccgaa agatatttga
     6721 aaggctgtac gaatacgtag ttatagagct cgtcaacgta ccacttgttg agcgaaccct
     6781 cgtaaagagg ctcgatggtt ttatcagacg cgatcctgcc ggggatcgag ggcttgaagt
     6841 agtaaaaata acaggccgcc gcgataccga ttagagcggc ggatatcgat aatacaatca
     6901 agttagtatg gctgaagaga tgatgccccg ccgatatgaa gttatccata gtgatggaag
     6961 aggaaacgaa tttgtcgaaa aggttcgact cgtgtatccc cacgagctct cccatgaact
     7021 ccggaacgcc catgtatcct ccaactatgg aaaggaaggc gaggaccatg agggggatcg
     7081 tcattgttgc gggggactcg tgtatgtggt cttttacctt atccgtgacc ctcgatttgc
     7141 cctcgaacgt gaggaggtaa agccttccca tgtagattgc ggtcaggacg acggtgatca
     7201 tgcctataat ccagtggatc gtgtatccgc gttcgtacac ggaacccagg atggcgtctt
     7261 tgctgaaaaa gcctgagaag aacgggatgc ccgctatggc cagagtgcct ataaggaatg
     7321 tgatcgcggt cgtgggaatg aattttctga ggccgcccat gtgtcttatg tcctgctcgc
     7381 ccgaaagagc gtgtatgacg cttcccgacc cgagaaagag gagcgccttg aagaacgcgt
     7441 gtgtcacgag gtggaagagc cccgccgtgt acgcccctac gccaatgccc atgaacatgt
     7501 agccgagttg gctgattgtg gagtaagcga ggactttctt tatgtcgttt tgtgttatag
     7561 ctatcgtcgc cgcgaggaac gaagtcagtg cagcgacagt taccacaacc atctgggccg
     7621 ttggggactg ggagtataat acgttgcacc gagcgatcat gtaggcgccc gccgtgacca
     7681 tcgtggcagc atgtatgagg gcgcttaccg gagtggggcc ggccatggcg tcgggcagcc
     7741 acacatataa aggtatctgg gccgacttgc cgactgctcc tacgaaaagc aggagagcta
     7801 tgaccgtgat cacggccgcg ggtaccgcgc tcatgaacgc ggggtcggac gccttgccga
     7861 atacctcctg ataattcacg gagccgaaaa cgaaaaatat aagaaagatt ccgagaatga
     7921 atccgaagtc ccctatcctg ttcacgacga aggctttcct gccggcggtc gcgttctccg
     7981 ggttctggta ccagaaaccg atgaggagga aggacgcgag cccgacccct tcccagccga
     8041 cgaacatcgg ggggaagctg tctcccaata ccagcgtgag catgaaaaag ataaaaatat
     8101 tgagatatgt gaagtacttg gaatatgcct cgtcgtggtg catgtacccg accgaataga
     8161 cgtggatgag gaagccgacc catgtgacga taagcgccat cacgcaggag agggtgtcga
     8221 agaggagcga gaagctcacg gtaaagctgc ccgagggtat ccattcgaag aggttctgaa
     8281 ttatctcacg ccgcgcggga tcgagcgaga gcagcttgaa aaagagcgat gtcgaaatga
     8341 gggcggatac gaggacgacg ccgcagccga ttatgcttac gaccattttc tccgtgtgct
     8401 cgtcgccgcg gagatacttc ttccagaacc cggaggcgaa gaagaggccg ttgacggccg
     8461 caccaaatag aggaatgaga ataatccaga ctatcatcta gtttctaacc ttttagtatt
     8521 tttaatatat cgacatcgag cgtctccctg tgcgcgtata cagccacgac gagggcgaga
     8581 cccacggcca cctcggccgc tgctacagtg atggacataa tcatgaacac ctgaccggtc
     8641 atatcgttgt gagccctcga gaacgcgacg aaggcgaggt tcgcggaatt gatcatgagc
     8701 tc
//
LOCUS       DASSJH010000241         5596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_384054, whole genome shotgun sequence.
ACCESSION   DASSJH010000241 DASSJH010000000
VERSION     DASSJH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384054"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VFJ67_05220"
     CDS             complement(<1..819)
                     /locus_tag="VFJ67_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7289822.1"
                     /translation="MKVKNIIVPLAFLMLVPALFSCAHATGYKSAPETADEPFESTDT
                     PKNEAYNHFAVSRMLFLDNRIEESLAELELAEQADPSSAYLKYNLALVYMTIGRMQDA
                     MAKLQESIKTDPDFAPTYTLLGKIYASSQDPKEREQSVGILERAVQLDPDDAESLLFL
                     GIMDTEAGNYESAEARFKKITELYPDNEKSYFFLGKLYYEKGDYARAEENYKKALDIN
                     PSFGSALVELALVYEQQGKMKESERIYQDVIVLFPHSLESYVRYGNFLFRVNRME"
     gene            complement(821..2251)
                     /locus_tag="VFJ67_05225"
     CDS             complement(821..2251)
                     /locus_tag="VFJ67_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET7289823.1"
                     /translation="MTYQKGDSIRWALFLFILLSCISAHAASSGENLLYIYSGGKKIG
                     YSYDSVKTEGGVTEAVERARIKVKLLDNTQDIATDARYRLDNGRLTSFDFRFESPSGA
                     VTARGEREGDAMRVKMSSLSGASEIVFDAPEGLIPASMLPARLKAEGFGIGREYTVPV
                     LDPMSVITASSAESFDTLHKVVGKESVEVPSLGAVEAWEVESTLSGTRVTTWISDDGR
                     VLKQIMPPGMTALRDEGGRVSDEGLTALDIEGATSIPSNVTIDDPRDLSFMKAEIGGI
                     VPGDGFDLSDGYRQTFDGNIVSIRAGDTSTVRTYELPNKDPRFGPYLAPENLIQSGDK
                     DIVSKSREILGEERDSLKAAVMLNAWVFSHLTKTGTAGIPNARDVLKTGSGDCNEHSA
                     LFAALARAAGIPTKTVSGTIYLDGRFYYHAWNEVYVGEWVAVDPTFGQVPADATHIKL
                     VEGDLNKSSVIMNAAGKINLKILEAS"
     gene            2697..3773
                     /gene="mqnC"
                     /locus_tag="VFJ67_05230"
     CDS             2697..3773
                     /gene="mqnC"
                     /locus_tag="VFJ67_05230"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HET7289824.1"
                     /translation="MEVRNIFGKVFAEERITRDEGLFLLKNADTIQMGALADRVRRKH
                     HPEGVVTFVADTNPNYTNVCDTECLFCAFWRPPGAHDAYTHSVDKVIEMMRTSYHNGA
                     TTVLLQGGHNPEITLDYYLEIIRRTRKEIPGLHLHAFSAPEISAIAKYSDLTTEEVLR
                     RFWDAGLRTIPGGGAEVLSNRVRKKISPLKIDADQWMKVTREAHLIGFRTTATMMFGH
                     YEEDEDILEHLERIRALQDETGNFLAFIPWTFKPKNTFLERKLPNETGGDRYLRVLAL
                     SRIYLDNFVHIQGSWFTQGAKIGSLSMFYGASDLGGTLFDENVLCCAENKLRSTVDEL
                     VHMIRSAGFVPAQRDTYYQILQRF"
     gene            complement(4017..4325)
                     /locus_tag="VFJ67_05235"
     CDS             complement(4017..4325)
                     /locus_tag="VFJ67_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289825.1"
                     /translation="MKVPREEISHIHISHIDEFIAARFIEAGGRPLAFRISGADDEGT
                     ILLEAIEPRSGPPEQEGIDFELSACECGGLGHEDAGIYTGRPVYTSSAYEFLDFLLGH
                     "
     gene            complement(4411..>5596)
                     /locus_tag="VFJ67_05240"
     CDS             complement(4411..>5596)
                     /locus_tag="VFJ67_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289826.1"
                     /translation="GGGYYGHHHHHHDKGHYYDNDYYAPGQHCVFVPEDKFSNRNAKL
                     YRVDGERNLTVMRNVKNVTNIKTVNNTIYNYGPDKTAVERASGQKLRPVNLVDSDLST
                     LRAGKGGRNLDNNNYKVFRPNIERKADETPFTGENNVLRRAENGRNTKGAGVNNAFTS
                     GEKNTGNNPGQGRSLEGPGNKSYGNTAPDNYGVIKKNTGRDAGPVAPDYGKRGDYPGG
                     KNTPDYNQGGPDKNATVTSRQKNSYPGQPVYNDQNRVKSNKAVNDRMKNNQSPVQVPQ
                     QKSVERNPYNKYRGPGGSLNTYQSSGKSSRQTYNKPALQQRNTYNPNGYKAQSQQAPK
                     YNYQPSQRQSNASSSKKSSGRSSSNTDRTQYTSPTINQGARSYNPGSTRSYNSSSFAG
                     RR"
BASE COUNT         1067 a         1592 c         1583 g         1354 t
ORIGIN      
        1 ctccatcctg ttgaccctga agaggaaatt gccgtaacgc acgtaggact cgagactgtg
       61 gggaaagagc actataacgt cctggtatat gcgctccgat tccttcatct ttccctgctg
      121 ctcgtacacg agcgcgagct cgacgagggc cgagccgaag ctcgggttta tgtcgagggc
      181 cttcttgtag ttctcttcgg cgcgggcgta atcgcccttc tcgtagtaga gcttgcccag
      241 gaagaagtag ctcttttcgt tgtcggggta aagctcggta atctttttga acctggcctc
      301 ggcgctctcg tagttgcctg cctccgtgtc cattatgccg agaaataaga gggattcggc
      361 gtcgtcgggg tctagctgga cggccctctc aagtatcccg acagactgct ccctctcctt
      421 cgggtcctgg gaggaggcgt atatcttgcc cagcagcgtg taggtagggg cgaaatccgg
      481 gtcggtcttt atggattcct ggagctttgc catcgcgtcc tgcatcctgc cgatagtcat
      541 gtaaacgagc gcgaggttat atttcaggta cgcggacgag gggtcggcct gctcggcgag
      601 ctcgagctcg gcgagggact cctcgatcct gttgtcaaga aaaagcattc gcgagacggc
      661 gaagtgattg tacgcctcgt tcttgggcgt gtccgttgat tcgaacggct cgtccgcggt
      721 ttcaggcgcg ctcttgtatc cggtcgcgtg agcgcaggaa aagagggccg gaacgagcat
      781 cagaaacgcg agcgggacaa taatattttt tactttcatt tcaggaggct tccagtatct
      841 tgagatttat tttacccgca gcgttcatta tgacggagct cttgttgagg tccccttcga
      901 cgagcttgat atgcgtcgca tcggccggga cctgaccgaa agtggggtcg acagccaccc
      961 actccccgac gtacacctcg ttccatgcgt gatagtagaa cctgccgtcg agatatattg
     1021 tacctgacac cgttttcgtc ggaattcccg ccgccctggc gagcgccgcg aaaagggcgg
     1081 aatgctcgtt gcagtccccg gagcccgtct tgagcacgtc gcgcgcgttg ggaattccgg
     1141 ccgtgcccgt cttcgtgaga tgcgagaaga cccatgcgtt gagcatgact gccgccttga
     1201 gcgagtccct ctcttccccg agtatctccc tcgatttcga cactatgtcc ttatcgccgc
     1261 tctgtatgag gttctccggg gcgagatacg gcccgaatcg cgggtctttg ttcgggagct
     1321 cgtatgttct gaccgtcgac gtgtcccccg cccggatcga gactatgttc ccgtcgaagg
     1381 tctgcctgta cccgtccgag aggtcaaagc cgtccccggg gacgatgccg cctatctcgg
     1441 ctttcatgaa gctcaggtcc cgcgggtcat cgatcgtgac gttcgaaggg attgaggtcg
     1501 ccccttctat gtcgagcgct gtcaggccct cgtccgatac ccgcccgcct tcgtcacgga
     1561 gggccgtcat gccgggcggc attatttgtt taagcacccg gccgtcgtcc gatatccatg
     1621 ttgtgacgcg cgtgcccgag agcgtggact ccacctccca tgcctcgacc gccccgagcg
     1681 aggggacctc gacactttcc tttccgacga ccttatgcag cgtatcgaag ctctcggcag
     1741 acgaggccgt tatcacggac atggggtcga gcacggggac cgtgtattcg cgcccgatcc
     1801 cgaagccctc cgcctttagg cgggccggga gcatggacgc cgggatgagg ccctcaggcg
     1861 cgtcgaaaac gatctctgac gcccccgaca gggagctcat cttcacgcgc atcgcgtccc
     1921 cttccctctc tccccgggcc gttaccgctc cggacgggga ttcgaacctg aaatcgaacg
     1981 aagtgagcct gccgttatcg agcctgtagc gggcgtcggt cgcaatgtcc tgcgtgttgt
     2041 cgaggagctt gacctttatt ctcgcccttt cgacggcctc cgtaacgccg ccttcggttt
     2101 ttacggaatc gtaggagtag cctatcttct taccgccgga atatatgtag aggaggtttt
     2161 caccggatga agccgcgtgc gcggagatgc acgaaaggag tatgaagagg aacagagccc
     2221 accgaattga gtcccctttc tggtatgtca tgttacggga ataatctaca cacctttgcc
     2281 cggggtctca atattttttg ttgaacgggg tatgggggcg cacaatgtgc gctcatatac
     2341 gattccttcc agccccccca tatgaaccag cgactgaggc ccaacccgtt cgccctgagc
     2401 tcgtcgaagg gttgaagggg tttgctctcc ctaatttgtc gcggctggaa gccgctccta
     2461 caaaatacta tcaccctcac cctacccctg tttagtatta tgcaacattt agcctgaagt
     2521 cgatgtactt cattgcctct gttcaggggc gaccgattgc gcgcttgctt ccaatcgtcc
     2581 ctcaagggag agggaaatcg ttggttaagc aggggagctc cggtaatata gcttacgagc
     2641 gcaggttctg cgccccgatt gacaccccgc gccattttag gttcactaga atgtatatgg
     2701 aagtaaggaa catattcgga aaggtattcg ccgaggaaag gataacgagg gacgagggtc
     2761 tctttctcct caagaacgca gacaccatcc agatgggcgc gctagccgac cgcgtgaggc
     2821 ggaagcacca cccggagggg gtcgtaacgt tcgtcgccga taccaacccg aactacacga
     2881 acgtctgcga tacggagtgc ctcttttgcg ccttctggcg tcccccgggc gcgcacgacg
     2941 cctacaccca cagcgtcgac aaggtcattg aaatgatgag gacgtcctac cacaatgggg
     3001 cgacaactgt gctccttcag ggcggacaca accccgagat cacactcgat tactaccttg
     3061 aaataataag gaggacgagg aaggagattc cgggcctcca cctccacgct ttctcggcgc
     3121 ccgagataag cgcgatagcg aaatactccg acctcacgac tgaagaagtg ctgaggaggt
     3181 tctgggacgc cggtctccgg acgatccccg gcggaggcgc ggaagtatta tcgaaccggg
     3241 tgaggaagaa gataagccct ctcaagatag acgccgatca gtggatgaag gtcacgcgtg
     3301 aggcgcacct gatcgggttc aggaccacgg ccacgatgat gttcgggcac tacgaggagg
     3361 acgaggacat actcgagcac ctcgaaagga taagggcgct ccaggacgag accgggaact
     3421 tcctcgcgtt catcccgtgg acgttcaagc cgaagaatac gttcctcgaa aggaagctcc
     3481 cgaacgaaac gggcggcgac aggtacctca gggtattggc gctctcgcgc atatacctcg
     3541 acaacttcgt ccacatacag ggctcgtggt tcacgcaggg ggccaagatt gggtctctct
     3601 ccatgttcta cggcgcgagc gacctcggcg gcacattgtt cgatgagaac gtgctctgct
     3661 gcgccgagaa caagctgaga tcgacagtgg atgagctcgt gcacatgata aggtctgcgg
     3721 ggttcgtgcc cgctcagagg gatacttact accagatact ccagaggttt tgattccacc
     3781 tgaattagac aattcaaaat acgaaggcag ggtaattacg ccttttcttt tttgtcattc
     3841 ccgacaccga tcgggaatct agtttttttc ttgttttatt taaaagatat ggattcccga
     3901 ttaagacact cgggaatgac agaacaaaaa attaggtccc ctcactcctc gaccgtattc
     3961 tcaaccgtga cgctccatgc gtttccggtg aactcgatgt aacagtcgaa ggagctttaa
     4021 tgtccgagga ggaagtcgag gaactcgtag gcggagctcg tatagacggg tctgcctgta
     4081 taaatacccg cgtcctcgtg gcctagaccg ccgcactcgc acgccgagag ctcgaaatct
     4141 atcccctcct gctcgggcgg gcccgaccgg ggctcgattg cctcaagcag aatcgtgccc
     4201 tcatcgtccg ctccgctgat cctgaaagcg agcgggcgtc cgcctgcttc gatgaatctc
     4261 gcggctatga actcgtcgat atgggatata tggatatggg agatttcttc gcggggaact
     4321 ttcactgatg agtatattaa cagaaggggc gcccgttttc acggccgccc ccggtcggaa
     4381 tttctaaaat gagtttttta ccgcttcagg ctaccttctt ccggcgaacg agctgctgtt
     4441 gtaagacctc gtgctgcccg gattgtaact ccgcgcgccc tgatttatcg tcggactggt
     4501 atattgagtc cggtcggtgt ttgaggacga cctaccgctc gattttttcg aggaggaggc
     4561 gttcgactgc ctctggctcg gctgatagtt atatttggga gcctgctgac tctgcgcctt
     4621 gtatccgttc gggttgtagg tgttcctctg ctggagcgcg ggcttgttgt acgtctgcct
     4681 gctgctcttg ccggagctct ggtacgtatt taatgagccg ccgggcccgc ggtatttgtt
     4741 ataagggttc ctttccacgg acttctgctg cgggacttga acaggcgact ggttattttt
     4801 catccggtcg ttaaccgcct tgttgctttt cacgcggttc tggtcgttat agaccggctg
     4861 tcccggataa gagttcttct gtctgctggt caccgtggcg ttcttgtcgg gtccgccctg
     4921 attgtagtcg ggcgtgttct tgccgccggg gtagtctccc cttttcccgt aatccggagc
     4981 gaccgggcct gcgtccctgc ccgtattctt ctttatcacg ccgtaattgt cgggcgcagt
     5041 gttgccgtat gacttatttc cgggcccttc gagcgacctg ccctgacccg gattgttgcc
     5101 cgtattcttt tcgccggacg tgaaagcatt attaacaccc gcccctttcg tattccgccc
     5161 gttttccgcg cggcgcaata cgttattctc gcctgtgaac ggtgtttcgt ccgctttcct
     5221 ttctatattc ggcctgaaca ccttataatt gttgttgtcg aggttcctgc cgcccttccc
     5281 cgccctgagc gtggaaaggt cggaatcgac cagattgacc ggcctgagtt tctgtccgct
     5341 cgcccgctcg acggccgtct tgtcagggcc gtagttgtat atcgtgttgt ttacggtctt
     5401 gatgttcgtc acgttcttca cgttgcgcat gaccgtgagg ttgcgctctc cgtccaccct
     5461 atagagcttc gcgttcctgt tcgagaattt atcctcgggc acgaacacgc agtgctggcc
     5521 cggagcgtaa tagtcgttgt cgtagtagtg gcctttatcg tgatggtggt gatggtgacc
     5581 gtaatatccc ccgccc
//
LOCUS       DASSJH010000242         3292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_59253, whole genome shotgun sequence.
ACCESSION   DASSJH010000242 DASSJH010000000
VERSION     DASSJH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59253"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..763
                     /locus_tag="VFJ67_05245"
     CDS             221..763
                     /locus_tag="VFJ67_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289827.1"
                     /translation="MVARCLYICVILNFLLLFGCAKGPLNVNEFVAPKAASANYEVVL
                     IPDFRKTDLEWVPYDSGTEIADMVAEQLRTQDKFKVISRTDKFQGAGDQKVLLVDGIV
                     SGYTRGCKYCEYIFHGFDDKGKMSVQVRVTLVDDKTGEILADVGIDGRAKPPGTGRSK
                     YIRVVDEIVKVIDSVNSGKS"
     gene            975..1994
                     /locus_tag="VFJ67_05250"
     CDS             975..1994
                     /locus_tag="VFJ67_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET7289828.1"
                     /translation="MKAKVLFFILGSVTGFFIAYTHYELVIAAAAAVLLGAVSTGIAL
                     VLERNIRHYTPKSFLGAAIGMSAAALCFFAVREVISGFSIPGEISALISAAFFLFLFH
                     AGITIGYRKGKETEQAAARGRVHTRTVTETKILDTSVIIDGRIADVAEAGFINGPMII
                     PKFIIKELQHIADSSEPIKRVRGRRGLDVLKRMQKDIPNVSVKITNHDFPNIKEADLK
                     LVELARRLKGIIITNDFNLNKVAGLQNVKVMNLNQLSNALKPVVLPGETMSIHVVKEG
                     KEENQGVGYLDDGTMVVVDEAKRYLGDHIEVSVTSVLQTPTGRMIFSRVKEEDSKHMV
                     SDYRG"
     gene            1987..2694
                     /gene="ispD"
                     /locus_tag="VFJ67_05255"
     CDS             1987..2694
                     /gene="ispD"
                     /locus_tag="VFJ67_05255"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET7289829.1"
                     /translation="MARISASAVIAAAGQGKRFGDGLPKQFLPLLGKPVLAYSVETFS
                     KSGLIDEIILVVPGDWSSFTKTDITNKLSTDKVIKIIAGGPERQDSVLNGFSSLSGKP
                     DIVVVHDGARPLVSPELLEEVISEASACGGAIAALPAGDTVKKASAEMYVGGTIPRDS
                     LWFAQTPQAFRYDVLKNALSAASRDGFLGTDESQLVERTGVNVKLVKGSPYNIKITTP
                     EDLRLGELILKERAAAK"
     gene            2761..3243
                     /gene="ispF"
                     /locus_tag="VFJ67_05260"
     CDS             2761..3243
                     /gene="ispF"
                     /locus_tag="VFJ67_05260"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET7289830.1"
                     /translation="MHRIGFGFDAHKFSEGRKLMLGGLEIPFGLGLSGHSDADVLTHA
                     ICDALLGALGDGDIGTHFPDSDPRYKGVSSLSFLREITHMLKRGGYEVGNIDCVIVCE
                     KPRIAPHIQAIKDSLSQVMGLPGNRIGIKATTTDGMGFTGRGEGIASYAVALIKPASA
                     "
BASE COUNT          854 a          829 c          927 g          682 t
ORIGIN      
        1 acgcgacgca cgcggtcatg aggcaggcga gggtggcggc aaggactggc tgccgggtca
       61 aaattatccg tgattcgagg cgggtccggt atctcatccg gccttttccg gggagcgcgg
      121 cgagaacccg cgggctcgcc cgcatcatac gaataacgac ggtactttaa caaattacaa
      181 tttcggatac aatataaccc acgaaaaacg gaggaacgta atggtagcca ggtgtttata
      241 tatatgcgta atcctaaatt tcctcttatt attcgggtgt gccaaaggcc cgctcaacgt
      301 aaatgaattc gtagcgccca aggctgcgag cgcgaattat gaagtagttc tgatcccgga
      361 cttcaggaaa acggatctgg aatgggtgcc ctatgattcc ggcaccgaaa tcgcggacat
      421 ggtggccgag cagctgagga cgcaggacaa attcaaggta ataagccgta cggacaaatt
      481 ccagggtgcc ggggaccaga aggttttatt ggttgacggt atagtcagcg gatacacacg
      541 cggctgtaaa tactgtgaat atatttttca tggttttgac gacaagggaa agatgtccgt
      601 ccaggtcaga gttactttgg tcgatgataa aacgggggag atcctcgccg atgtcggcat
      661 agacggaagg gccaaacccc cgggcaccgg aagaagcaag tatatcaggg tcgttgatga
      721 aattgttaaa gtgatcgact ccgtgaacag cggtaagtct tgacagcttt ataataaaaa
      781 aataacaatc tcatctcctt ttgcggattc cggtaccagt aatcgtaacc acacccggtt
      841 ttaagacgga tatcgatgtg attcgcggtt gaaacgaaga gctctttcgt cagcttgtga
      901 aaatttagcg attacttata cttagtcata accgaatcca ttctgaaggg gacagggatt
      961 gaggtgaggg agaaatgaaa gcaaaagtac tattctttat tctaggcagc gtgacggggt
     1021 tttttatcgc gtacacgcat tacgagctcg ttatagcagc cgcggcagcc gtccttcttg
     1081 gcgcagtctc tacaggaatc gctctcgtac tcgaacgcaa tatcagacac tataccccga
     1141 aatcgtttct cggagccgca ataggcatgt cggctgcggc tctctgcttt ttcgccgtca
     1201 gggaggtcat ttcgggtttc tctattcccg gggagatatc ggcccttata tcggccgcgt
     1261 tcttcttatt tcttttccac gccgggataa cgataggcta caggaaggga aaggaaacgg
     1321 agcaagccgc cgccagggga cgtgtccata cgaggacagt gaccgagacg aagatactcg
     1381 acacgagcgt catcattgac ggcaggatag ccgacgtagc cgaagcgggc ttcataaacg
     1441 ggcctatgat aatccccaag ttcatcatca aggagctcca gcatatcgcg gattcctcgg
     1501 agcctataaa gcgcgtaagg ggaaggaggg gcctcgacgt cctgaagaga atgcagaagg
     1561 acataccgaa cgtatcggtc aagataacta accacgattt ccccaacatc aaagaggccg
     1621 atctcaagct cgtagagctc gcgaggaggc tgaagggcat aatcatcacc aacgacttca
     1681 acctcaacaa agtggccggc ctccagaacg tgaaggtgat gaacctgaac cagctctcga
     1741 acgcgcttaa gcccgtcgta cttcccgggg agacgatgag catacacgtc gtcaaggaag
     1801 gcaaggaaga gaaccagggc gtaggttatc tggacgacgg cactatggtc gtggtggatg
     1861 aagccaaaag gtatctgggc gaccacatag aagtatccgt cacgagtgtg ctgcagaccc
     1921 cgaccgggag aatgatattc tcgagggtca aggaagaaga cagcaagcac atggtttcag
     1981 actaccgtgg ctaggatcag cgcctcggcc gtcatagcgg cggccggtca gggaaaaagg
     2041 ttcggggacg gattgccgaa gcagttcctg ccgcttctgg gaaagccggt actcgcgtac
     2101 tcggtcgaga cgttctcgaa gtcaggtctc atcgacgaga taatactggt cgtgccgggc
     2161 gattggtcgt ctttcacgaa gacggatata acgaacaagc tctccacgga taaagtaata
     2221 aaaattattg caggcggccc ggaaaggcag gactccgtcc ttaacgggtt cagctccctc
     2281 tcggggaaac ccgatatcgt cgtggtgcac gacggagctc gcccgctcgt ctcgccggag
     2341 cttctcgaag aggtcataag cgaagcctcc gcgtgcggag gggccatagc cgccctcccg
     2401 gcgggagata cggtaaagaa ggcttccgcc gaaatgtacg tcggcggcac cataccgaga
     2461 gattccctct ggttcgcgca gactcctcag gctttcagat acgatgtcct taaaaacgcc
     2521 ctctctgcgg cttcccgcga cggctttctg ggaacggacg agtctcagct cgtcgaaaga
     2581 acgggtgtaa acgtgaagct tgtcaaaggc tccccctaca atataaagat tacgactcct
     2641 gaagacctca ggctgggaga gctcatatta aaagagagag cggcggcaaa ataaaacgta
     2701 tccgcagctt taaaatgcgg attcgcccgt ttatgattac acgcattgac ggtccaggct
     2761 atgcacagga taggattcgg cttcgacgca cataaattct ctgaaggcag aaagctcatg
     2821 ctcggcggtc tcgagatacc gttcggcctc ggcctctcgg gtcattcgga cgcagacgtg
     2881 ctcacgcacg cgatatgcga cgcgctcctc ggggcattgg gtgatggaga cattgggact
     2941 cattttccag acagcgaccc ccgctacaaa ggcgtgtcga gcctctcgtt cctgagggag
     3001 ataacgcaca tgttaaaaag gggcggatac gaggtcggga acatcgactg cgtcatagtc
     3061 tgcgaaaagc ccaggatcgc ccctcacatt caggcaatta aagactcact ctcccaagtc
     3121 atgggacttc ccggaaacag gatcggaata aaggccacga ccaccgacgg catgggtttt
     3181 acgggcaggg gagaaggtat cgcgtcctat gccgtagctt tgataaagcc cgcgtcggcc
     3241 taaacgtcgg tcctatcggc gccggcgaat gaatcaaatc aggcaagcgc cg
//
LOCUS       DASSJH010000243         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1137468, whole genome shotgun sequence.
ACCESSION   DASSJH010000243 DASSJH010000000
VERSION     DASSJH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137468"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFJ67_05265"
     CDS             <1..557
                     /locus_tag="VFJ67_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7289831.1"
                     /translation="QNHIMKFWSHVKIGTTQAQRKLFHKIGRARKDLGIRHEELTESD
                     VSRVAEFFGVRDKDVIDMEMRMASRDFSLDTSFDEDNSITYLDTVSDSGSNQEDVLES
                     IEQAELANEGLRAGFNKLTPREKRVIESRYLSTPQGKLRELGDELGISKERVRQIEVQ
                     ALKKLRGEVETSVTQGNEYGTENR"
     gene            580..2529
                     /gene="dnaK"
                     /locus_tag="VFJ67_05270"
     CDS             580..2529
                     /gene="dnaK"
                     /locus_tag="VFJ67_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET7289832.1"
                     /translation="MAKSGKIIGIDLGTTNSVVAIVEGGEPKVIINEEGSRITPSVVA
                     FTKDGDILVGGPAKRQAVVNPENTIFSVKRLMGRRYEEVEGEAKRLPYKIVKTKNDDA
                     WVEVMGKQYSPPQISSHVLMKLKKAAEDYVGGEIKEAVITVPAYFNDSQRQATKDAGK
                     IAGLEVKRIINEPTAAALSYGFDKKSEGTIAVYDLGGGTFDISILEVGDNVIEVKSTN
                     GDTHLGGDDFDRLIMDYIITEFKKESGIDLGNDKMALQRLRQESEKAKVELSNTLETE
                     INLPFITADATGPKHLVMKITRSKFEQLIDEKVKGTLEPCRQALKDAGVKAGDIDEVI
                     LVGGSTRIPLVQKVVTDFFGKEPHKGINPDEVVAMGAAIQGAVLGGEVRDVLLLDVTP
                     LSLGIETLGGVMTKIIERNTTIPTKKSQVFTTAEDSQTSVEIHVLQGERQMAGDNRTL
                     ARFILDGIPPSARGIPQVEVTFDIDADGILHVSAKDLATQKEQKVRVEASSGLSKDEV
                     DKMVRDAEEKSEDDKKRHDLVEKRNRLDELIYRTEKSFKEFEGKLSEDDKKELEEALE
                     QGRKAVKGNEASEIESATDKITHASHKLAEFMYKQQSQGGGDGAHQAESGGEAPGEAP
                     QGEPGGEAEGGKDGDDVIDAEFTEK"
     gene            2721..2924
                     /gene="rpmE"
                     /locus_tag="VFJ67_05275"
     CDS             2721..2924
                     /gene="rpmE"
                     /locus_tag="VFJ67_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET7289833.1"
                     /translation="MKADIHPEYKDVTISCSCGAQYKTKSTRFEDFTVEVCSNCHPFY
                     TGKERAAESKGRVERFRKKYSKN"
BASE COUNT          860 a          749 c          933 g          491 t
ORIGIN      
        1 tacagaacca cataatgaag ttctggagcc acgtgaagat agggaccacc caggcgcaga
       61 ggaagctctt ccacaagatc ggcagggcca gaaaagacct cggcatccgg cacgaagagc
      121 tgaccgagtc cgacgtcagc agggttgccg agtttttcgg ggtcagggac aaggacgtca
      181 tagacatgga aatgaggatg gcgtcgaggg acttttctct cgatacgtcc ttcgatgaag
      241 acaattcgat cacgtatctc gacacggttt cggacagcgg ttcgaaccag gaagatgtgc
      301 tcgaatcgat agaacaggcg gagctggcga acgaagggct cagggcggga ttcaacaagc
      361 tgacgccgcg cgagaagcgc gtcatcgaaa gcaggtatct ctccacgccg cagggcaagc
      421 tgagggagct cggcgacgag ctcggtatct caaaggagag agtaagacag atagaggtcc
      481 aggctcttaa gaagctgaga ggcgaggtcg aaacttcggt tactcaggga aacgaatacg
      541 gtacggaaaa cagataacta agatcaggag gctattaaaa tggcgaaaag cggcaagata
      601 atcgggatcg acctcggaac gaccaactcg gttgtggcca tagtagaggg cggagagccg
      661 aaggtaatca taaacgaaga ggggtcgagg ataaccccgt ccgtggtggc gttcacgaag
      721 gacggcgaca tcctcgtcgg cggacccgcc aagagacagg cggtcgtcaa tcccgagaac
      781 actatattct cggtaaagag gctcatgggc agacgctacg aggaagttga gggcgaggcc
      841 aagagactcc cttacaaaat agtaaagaca aaaaacgacg acgcatgggt cgaggtgatg
      901 gggaagcagt attctccccc gcagatatcc tcgcacgtgc tgatgaaact gaagaaggcg
      961 gccgaggact acgtcggcgg cgagataaag gaagcggtca tcaccgttcc cgcctatttt
     1021 aacgacagcc agagacaggc gaccaaggac gccggcaaga tcgcgggact cgaggtcaag
     1081 aggataataa acgagcccac ggcggcagcg ctctcctacg ggttcgacaa gaagagcgag
     1141 ggcacgattg ccgtttacga cctgggcggc ggtacgttcg acatatcgat actggaagtc
     1201 ggcgataacg tcatagaggt aaagtccact aacggcgaca cgcacctggg cggagacgac
     1261 ttcgacaggc tcataatgga ctacataatc acagaattca aaaaggaatc gggcatcgac
     1321 ctcgggaacg acaagatggc gctccagagg ctgagacagg aatccgagaa ggcgaaggta
     1381 gagctttcga acacgctcga gaccgagatc aacctgccgt tcataacggc ggacgccacg
     1441 gggcccaagc acctcgtcat gaagataacg cgctccaagt tcgagcagct catcgacgaa
     1501 aaggtcaagg gcacgctcga gccctgccgt caggccctga aggacgccgg tgttaaagcg
     1561 ggcgacatcg acgaggtaat actcgtcggc ggatcgacca ggataccgct cgtccagaag
     1621 gtggtcacgg atttcttcgg gaaggagccc cacaagggta ttaacccgga cgaggtcgtg
     1681 gcgatgggcg cagctataca gggcgcggtc ctgggcggcg aggtaaggga cgttctactt
     1741 ctcgacgtca caccgctctc tctcggtatc gagaccttgg gcggcgtcat gacgaagatc
     1801 atagaaagga acaccaccat ccccaccaag aagagccagg tattcacaac ggccgaggac
     1861 agtcaaacat cggtcgagat acatgtgctc cagggcgaga ggcagatggc gggagacaac
     1921 aggacgctcg cgaggttcat actcgacggt atcccgccgt cagcgagggg gataccccag
     1981 gtagaggtca cgttcgatat agacgccgac ggcatactgc acgtctcggc caaggacctc
     2041 gccacacaga aagagcagaa ggtcagggta gaggcttcga gcggtctttc caaggacgag
     2101 gtcgacaaga tggtgaggga cgccgaggag aagtcggagg atgacaagaa gcgccacgat
     2161 ctcgtggaaa agaggaacag gctcgacgag ctcatataca ggacagagaa gagcttcaag
     2221 gaattcgaag gcaagctctc ggaggacgac aagaaggagc tcgaagaggc cctcgagcag
     2281 ggcagaaagg ccgtaaaggg caacgaagcc tccgagatcg agtccgctac ggataagata
     2341 acccacgctt cccacaagct cgccgagttc atgtacaagc agcagtccca agggggcggt
     2401 gacggagcgc atcaggcgga gtcaggcggt gaagccccgg gagaggcgcc tcaaggcgag
     2461 ccgggcggtg aggccgaggg cggtaaggac ggcgacgacg tgattgacgc cgagttcacg
     2521 gaaaaataag taaccgagag agcatttacg aaactcaaaa agcataaaag gcggcccccg
     2581 gccgcctttt tgtttgtcgg ggctgctcag attaactatt gaacagccgg catttaaatg
     2641 ttaatttaat attaagcagg tttgggtttt tataagatct aagacgtatt gcccgcgcat
     2701 aggcataagg aggtatcaca atgaaagcgg atattcatcc ggaatataaa gatgtcacta
     2761 taagctgctc ctgcggcgcc cagtacaaga ccaagtccac ccgtttcgaa gacttcactg
     2821 tagaggtgtg ctccaactgt caccccttct atacgggtaa ggaaagggcg gcggagtcca
     2881 agggcagggt cgaaaggttc aggaaaaagt actccaagaa ctagccccgt aacgccggca
     2941 aacaacacta tcagcttcag gcaattcatt tcataggaga cgacaaaggg ctttgatgaa
     3001 gatttttgtc aatgacagac cacggaggta atc
//
LOCUS       DASSJH010000244         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_595895, whole genome shotgun sequence.
ACCESSION   DASSJH010000244 DASSJH010000000
VERSION     DASSJH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595895"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFJ67_05280"
     CDS             <1..359
                     /locus_tag="VFJ67_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HET7289834.1"
                     /translation="GHPMDAKRALAFELTSWLHGLESAESAAQDFATKFSQREFPEDA
                     RAVSIKRGDGKTVLDLVVESSEKLKSRNEAKRLIAQGGVSVNGERVSDINSPMPGAGT
                     YQMRIGKKEFVLVKIS"
     gene            complement(359..741)
                     /gene="rnpB"
                     /locus_tag="VFJ67_05285"
     ncRNA           complement(359..741)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFJ67_05285"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(751..1140)
                     /locus_tag="VFJ67_05290"
     CDS             complement(751..1140)
                     /locus_tag="VFJ67_05290"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HET7289835.1"
                     /translation="MIHIDGAARGNPGEAGIGVLIEEENGGSREVKKYLGTRTNNQAE
                     YSALITALESSRDYKNREIRIFTDSLLLANQMNGFWKVKHPEIKELYSKAKGLIEGFS
                     RVTIKHIPREENTEADRLANLAIDEYL"
     gene            complement(1175..1903)
                     /locus_tag="VFJ67_05295"
     CDS             complement(1175..1903)
                     /locus_tag="VFJ67_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET7289836.1"
                     /translation="MRDQILVLDTLQRIDMELSQLEKNLKEYPEKISTYESEIEAFRE
                     RLETSKKIKDEILKNKSRIELDVAQNEDTIKKSEQKLFEIKTHKEYEALQKQIAEAKR
                     NNSVLEERLLQEMEKLEGLESRISKEEEELGNKENEYSSRISDFKEKIEELKVLYLPK
                     TEEKKKIASGLDRDLLSVYERVKKRNGYVLALAKNEVCTGCNMNIPPQLFNEVLTHSR
                     LIQCPNCLKFLYTESEIKSEAKTG"
     gene            complement(2059..2132)
                     /locus_tag="VFJ67_05300"
     tRNA            complement(2059..2132)
                     /locus_tag="VFJ67_05300"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2096..2098),aa:Ile,seq:cat)
     gene            complement(2274..3161)
                     /locus_tag="VFJ67_05305"
     CDS             complement(2274..3161)
                     /locus_tag="VFJ67_05305"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET7289837.1"
                     /translation="MKKPLLTLFFISTIILLIPLSAFPASPPGKKNGDDTAGEASQEK
                     NDILSVEVTNTDLQLVLLGTLTSGGVPKAMIKNPVTGELGSYVPGDVLDLVSSETVNV
                     VEISNCAVLIERSGKYETMECRNKMPTVVFNKPSALARYKLILPEDGGTKFIFSDEFT
                     TDYDKEIADLSEKHGVDPYLVKAVIKAESNFDPNAVSPKNAQGIMQLIPETANDYGVG
                     DPFDAKDNMEGGVQYLKDLLDYFDGDMSLSLAAYNAGKGAVIKHGFTIPPYPETTDYI
                     AKVLGYYKLLKAKSYALKR"
BASE COUNT          644 a          841 c          837 g          926 t
ORIGIN      
        1 aagggcaccc tatggacgcg aagagggcgc ttgcgttcga gctgacgtcg tggctccacg
       61 ggctggagag tgcggagtcg gccgctcagg attttgcgac caagttctca cagcgtgagt
      121 tccccgagga cgcgagagcg gtctcgatta agaggggtga cggcaagacc gtgctcgatc
      181 tcgtcgtcga gtcctcggag aagctcaaga gccggaacga ggcgaagaga ctgatagcac
      241 agggcggcgt gagcgtgaac ggcgaaaggg tctccgacat caattcgccc atgcccgggg
      301 cgggaaccta ccaaatgagg atagggaaga aagagtttgt gctcgtaaaa ataagttaga
      361 agcaagcgag cctgtaagcc gaattctgtc gaggacaatc attcatctgg gacatccatt
      421 actgaatgcc tcttgcaaca tacccggaag catcggacgg gccgtccttt atttcacgag
      481 gaaaagcgct tccctatttt gtcttactcc gggcggggtt taccatgccg cccgctgtcg
      541 ccagcaggcg cggtagtctc ttacactgcc ttttcaccct taccggcctt ccgtaaccgg
      601 aaagcttggc ggtatgtttt ctgtggcact ttccgttgcc tcacggcacc tgggcgttac
      661 ccagcgccct gccctgcgga gttcggactt tcctccagcc ccggcgtttc cgccgcagcc
      721 ggcgactgtc cggctcactt gcttcttcat ttaaagatat tcatcaatgg cgagattcgc
      781 aagcctgtcg gcttccgtat tctcttcacg ggggatgtgt ttgatcgtaa cccggctgaa
      841 tccttcgatt aagcccttgg ccttactgta aagctctttt atttcgggat gcttcacctt
      901 ccagaagccg ttcatctggt tggcgagcag gagcgagtcg gtaaatatcc ggatttccct
      961 gttcttataa tcacgggacg attcgagggc cgttatgagc gccgagtatt cggcctggtt
     1021 gttcgtacgt gtgccgagat acttcttcac ctcgcgcgag ccgccgttct cctcttctat
     1081 caggacccct atccctgcct cccccgggtt tcccctggcc gctccgtcga tgtgtatcac
     1141 tatgcggcct ccgccggacg gtttgaaggg aaggctatcc tgtcttggct tcactcttga
     1201 tctcgctttc agtgtaaagg aatttcagac agtttgggca ttggatcagt ctcgagtggg
     1261 tcagcacttc attaaacagc tgaggcggta tgttcatatt gcagccggtg catacttcgt
     1321 tcttcgcgag ggcgagcacg taaccgtttc ttttcttcac ccgctcgtat acggacagga
     1381 ggtccctgtc gagcccggag gcgatcttct ttttctcttc cgtcttgggc agatagagga
     1441 ctttcagctc ctctatcttt tccttgaaat ccgagatcct ggagctgtac tcattctcct
     1501 tgttcccgag ctcttcttct tccttcgata tgcgggattc caatccctcg agcttttcca
     1561 tctcttggag cagtctttct tcaagcaccg agttattccg tttcgcctcg gcgatctgct
     1621 tctggagggc ttcgtattcc ttatgggttt tgatttcgaa aagtttttgc tcggactttt
     1681 ttatcgtatc ttcattttga gcgacatcga gctcgatgcg cgacttattt ttaaggatct
     1741 cgtcttttat tttctttgaa gtctccagcc tttctctgaa agcctcgatc tcactttcgt
     1801 aagtcgagat tttttcgggg tattctttta agtttttttc cagttgactt agctccatgt
     1861 ctattcgctg gagcgtatcg agaaccaaaa tctggtctct catttttttc tccttttttt
     1921 tatcgcttta ttttcggaaa tattggtttt aaaaagtctg gctggaaaca ggggaacagc
     1981 tatttggaat gatatttgag gaattattct gataatgtcg tctctgcccg ttaaaagaaa
     2041 catgacctct tctaagtgtg ggcccacccg gactcgaacc agggaccaac ggattatgag
     2101 tccgccgctc taaccaactg agctatgggc cctctgaact tatttagtga atttagaagt
     2161 ctagataaag ttaaggggtt tgtcaaacca gcacgctccg gaaattctaa aatcagctcg
     2221 tgggtatttg gtgctgaacg cgcggctact gcggctctcg cgttcatgca gggctatctt
     2281 tttaatgcgt agctctttgc cttgagcaat ttatagtatc cgagcacttt cgcgatgtaa
     2341 tcggtcgttt ccggataagg gggtatggta aagccgtgtt ttattaccgc tcccttgccc
     2401 gcattatacg cggcgagaga aaggctcatg tctccgtcga agtagtcgag aaggtcctta
     2461 agatactgca cgcctccctc catattgtcc ttggcgtcga acgggtcgcc cacgccgtag
     2521 tcgttcgccg tttccggtat gagctgcatt atgccctgcg cgttcttggg cgatacggcg
     2581 ttagggtcga agttcgactc cgctttgatg acggccttta cgagataagg gtcgaccccg
     2641 tgtttctcgc tcaggtcggc tatttccttg tcgtaatccg tcgtgaattc gtcggagaag
     2701 atgaacttgg tcccgccgtc ctcgggcagg atcagcttgt acctggcgag ggctgaaggt
     2761 ttgttgaaca cgacggtcgg cattttgttg cggcattcca ttgtctcgta tttccccgac
     2821 ctctcgatga gcaccgcaca gttggatatc tcgaccacgt ttacggtctc cgagcttacg
     2881 aggtcgagca cgtctccggg gacgtacgac ccgagctccc ccgtaaccgg gttctttatc
     2941 attgccttcg gaactcctcc gctcgtgagt gtcccgagaa ggacgagctg gaggtcagtg
     3001 ttcgttacct cgaccgacaa tatgtcgttt ttctcctggg acgcctctcc ggcagtatcg
     3061 tctccgtttt ttttgcccgg cgggctagcc ggaaaagcgg ataaaggaat gagtagtatt
     3121 atcgtggata taaagaagag tgtcagaaga ggcttcttca tcgagtgaga tactcgtttg
     3181 agactacttt cagaataaat gataaagcgc caaaagtaaa taaagttttt aagtctctaa
     3241 gacgcccg
//
LOCUS       DASSJH010000245         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_276037, whole genome shotgun sequence.
ACCESSION   DASSJH010000245 DASSJH010000000
VERSION     DASSJH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276037"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFJ67_05310"
     CDS             <1..711
                     /locus_tag="VFJ67_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET7289838.1"
                     /translation="LPIPGHNIPMAIIGTFILAFGWFGFNPGSTLAGTDLRIGVVATN
                     TMLASAAGAFTAMVIMWTRFGKPDPSMMANGLLAGLVAITAPCAFVNSVSAVIIGVIA
                     GALVVGSVFFVERVLKVDDPVGAVSVHGTCGAWGVLSLGIFADGVYGDGWNGVAGPVK
                     GLLYGDPSQLMAEVIGVITNFVVVFVLAYVFFKICDALIGLRTSPETEIQGLDLPEMG
                     VEGYAAYTDYPQPHVMEK"
     gene            981..1694
                     /locus_tag="VFJ67_05315"
     CDS             981..1694
                     /locus_tag="VFJ67_05315"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET7289839.1"
                     /translation="MTKPEVEKAGFINRAIGRLRSRRFAYLAKSLVILILAYPYLEVN
                     IRGQLLLTIITIFVMMSLIIAVSDSKRSIIITLCLGLPWFASLIVNFPVFENAQTVFI
                     RKEILFAVLLFSYTTFKIFMHLLRSREVTSEILFASVCVYLLIGLTWSSFYIFIDYIY
                     PHSFIDTSDLIPINAPRILFFSYITLTTVGYGTLIPVSDPARSLALLEAIIGQLYLAI
                     LVARLVGLHISKPRATDQS"
     gene            1971..>3323
                     /locus_tag="VFJ67_05320"
     CDS             1971..>3323
                     /locus_tag="VFJ67_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7289840.1"
                     /translation="MHNSSLKLRARKILSHLRTYRRFYIVLAAFSFNIFTAFSNFSTV
                     HIKQYSYGGFYLYGLFFILCLPLRLVPGLLICGAGLSYALWWINEEKSGLTQLPLTVL
                     DFRITMSNPEGVIEAIKAPGWTLYVIAAAAVIIVLVAVWAAVSTFRSITSGKNKYAYR
                     RLTLNLLAAALLLVSANIFLTQFLNVVRRLVYEDDYMWESVRFSGLSRTIGIWGFLFY
                     SYQLERIDSGDFLNSSRGAPPPTNDEIAEAVREYINIGGNPRHKQPNIVVVQAESTFN
                     PEDAFILSKPIHNKLFEPNKYTQAVGPMFVNAVGGGSWITEFEFVTGIDSRLFGYSGF
                     YTHSSLSPYVKESFATYLKNHGYVTEAYYVTEGEFYNGSNAYRNYGFDHFYGNIGPAG
                     WGTTDEQVADVVIGSSKPEEAPFFKFIITIENHSPHTCKDYTSKEKFVTTFEGSDEFN
                     E"
BASE COUNT          779 a          919 c          840 g          785 t
ORIGIN      
        1 ctcccgatcc cgggacacaa catcccgatg gcgataatcg gtacgttcat actcgcgttc
       61 ggctggttcg gattcaaccc gggcagcacg ctcgcgggca ccgacctcag gatcggcgtc
      121 gtcgcaacca acacgatgct cgcgtcggct gccggagcgt tcacggccat ggtcattatg
      181 tggaccaggt tcggcaagcc cgatccaagc atgatggcga acgggttgct cgcgggactc
      241 gtcgctataa cggcgccgtg cgcgttcgtg aacagcgtct cggccgttat aatcggcgtc
      301 atagccggcg ctctcgtagt tggaagcgta ttcttcgtcg agcgcgtgct taaagtggac
      361 gaccccgtag gagcggtttc cgtacacggg acctgcggag cgtggggcgt cttgagcctg
      421 ggtatcttcg ccgatggagt ttacggggac ggatggaacg gtgtagcggg cccggtcaag
      481 gggctcctct acggcgaccc gtctcagctc atggccgagg taataggcgt catcacgaac
      541 ttcgttgtcg tcttcgtcct cgcttatgtc tttttcaaga tatgcgatgc cctgataggc
      601 ttgagaacaa gccctgaaac agaaatacag gggctcgatc tgccggagat gggtgtcgag
      661 ggttatgcgg cgtataccga ctaccctcag cctcacgtga tggaaaaata ggatacaatc
      721 cacggagagc cgcggttcaa tgtgccgcgg ctctgccttt ccccttaaag atagccgccg
      781 gtgtatccgc accggcgggc tttttttata taaacgaaat agagatcgcg tccattccga
      841 ggggacgatg agagagcgga gtgtttgctt ccttcccgta ttccccgcac gtttttgccc
      901 ggactgtcaa actccactac attcccgcaa aaatagtagt tatagttaaa ccttattcgc
      961 gcggccaggc ggagataccg atgaccaaac ccgaagtgga aaaagccggt ttcattaaca
     1021 gggcgatcgg ccgtctcaga agcaggaggt tcgcctatct cgccaaatcg ctcgtgatac
     1081 tcatccttgc ttacccctac ctcgaagtga acataagggg tcagctcctg ctcacgataa
     1141 taacgatatt cgtcatgatg tcgctcataa tcgcggtcag cgatagcaaa aggagtataa
     1201 taatcacgct ctgcctgggc ctgccgtggt tcgcatcgct tatcgtcaac ttcccggtgt
     1261 tcgagaacgc acagaccgtg ttcataagga aagagatact cttcgcggtc ctcctcttct
     1321 cctacacgac gttcaaaatc ttcatgcatc ttttaaggag ccgcgaggtg acttccgaga
     1381 tactcttcgc ctccgtgtgc gtttacctgc tgatcgggct cacctggtcg tccttctata
     1441 ttttcataga ctacatatac ccccactcct tcatagacac gagcgacctg atcccgatca
     1501 acgcgccgag gatactcttc ttcagctaca taaccctgac cacggtcgga tacggcaccc
     1561 ttatcccggt ctccgacccc gcacgctccc tcgcgctcct cgaggcgata atcggccagc
     1621 tatacctcgc catattggtc gcccgcctgg tagggctcca catatccaag cccagggcca
     1681 cggaccagag ctagggcccc gtcccgcttt gtcggctact ccttacaccg ggagatgcag
     1741 atagcaattc aacaccgtat tatccatcaa ctccgattat gattgaacag aatccggcgc
     1801 tataatataa tatttacagg gtataggacg ccaagagaca cggggataaa gtaataacgc
     1861 atgttcgcgt tccgattata tttttcaaca cttacggtaa caggttcaga ccacgggacc
     1921 gcacatcttc tacgccggaa tcaaactctc ctctgcccgg gtgatcgggg ttgcataatt
     1981 cctctctgaa actaagagcc cggaagatac tgagccatct aaggacgtac cggagattct
     2041 atatagtcct ggccgcgttc tccttcaaca tcttcaccgc tttttccaat ttttcgaccg
     2101 tacacataaa acaatattca tacggagggt tttatttata cgggctcttt ttcatcctgt
     2161 gtttgccgct ccgccttgtg cccggtctcc ttatttgcgg cgcggggctt tcctacgcgc
     2221 tctggtggat aaacgaagaa aaaagcggcc tgacgcagct cccgcttacg gttctcgact
     2281 tcagaattac gatgtcgaac cccgagggag ttatagaggc aatcaaagcg cccggctgga
     2341 cgttgtatgt cattgccgcc gcggcagtaa ttatagtcct cgtagcggta tgggcagcag
     2401 tatcaacttt ccgcagcatt acatccggaa aaaataaata tgcctacaga aggctgactc
     2461 tcaacctgct ggccgccgca ctattgctgg tatcagccaa tatcttcctt acacaattct
     2521 taaacgtagt acggaggctc gtatacgaag atgactacat gtgggaaagc gtgcgcttct
     2581 ccggcctttc gagaacaata ggcatctggg gatttttatt ctactcgtat caactcgagc
     2641 ggatagattc aggagacttc ttgaattcga gtcgcggcgc gccgcccccg acgaacgacg
     2701 agatcgcgga agcggtcaga gagtacataa atattggtgg gaacccgcgg cataagcaac
     2761 cgaatatcgt ggtcgtgcaa gccgaatcga cttttaatcc cgaggacgct ttcatactga
     2821 gtaaacccat acacaacaag ctgttcgagc cgaataaata cactcaggct gtcgggccga
     2881 tgttcgtcaa cgccgtcgga gggggcagct ggataacaga gttcgagttt gtcacgggca
     2941 tagactcgcg tctcttcggt tactccggat tttataccca ttcgtccctc tcgccttacg
     3001 taaaagagtc gttcgcgaca tatttaaaaa accacgggta cgttacagaa gcctactatg
     3061 taaccgaggg cgaattctat aacggctcca acgcatacag gaactacggc ttcgatcact
     3121 tctacgggaa tatcggtccc gcgggctggg gcactacgga cgagcaggtg gcggatgttg
     3181 tgatcggatc gtcgaaaccg gaagaagcgc cgttctttaa atttattata acgatcgaga
     3241 accattcccc gcacacttgt aaagattaca ccagcaagga aaagttcgtt acaacgtttg
     3301 agggctcgga cgagttcaac gaa
//
LOCUS       DASSJH010000246         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_596875, whole genome shotgun sequence.
ACCESSION   DASSJH010000246 DASSJH010000000
VERSION     DASSJH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596875"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFJ67_05325"
     CDS             complement(<1..665)
                     /locus_tag="VFJ67_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7289841.1"
                     /translation="MKVTILGSATSTGVPIIGCNCPVCTSASPRNKRTRCSVFVEAAG
                     RNILIDTSTDLRCQALRHGITKLDAVLYTHSHADHTHGIDELRVYNFVNKTSIRCFGN
                     AQTVDNIRNNFKYIFDGVESAGGKPNLTLTPVSGKFDFDGVEIIPVDIYHAEWTILGY
                     RIGGMAYLTDCSGIPEGSWDKLRDLEVLIISALRYKPHPAHLNVEQAVEVARKLSPGL
                     TVFT"
     gene            805..2529
                     /locus_tag="VFJ67_05330"
     CDS             805..2529
                     /locus_tag="VFJ67_05330"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7289842.1"
                     /translation="MSTKPRFAAIVASFLLITLISSNSYAADKGEDLYKETFQLYKSL
                     AGSQEKISDKEIWDTIARAFYSIYINYPQSDKAPNALFISGKMYEEMGERFGSGDDLE
                     TAVDYSRLFVKKYPESSLADDAQLRVARIVEKDSRSDAYLEYEKITTDFPNGDMNTIA
                     KNKTAELSPFKPAVRKADTGDEPAQPDSAADSKSGLVSVSQIRHWSTDNFTRVVIHVD
                     KETPFKSMFLKEDKANGKPPRLLVDIYGTKVDKKLYVEPITKGLLEEIRFARNTKDSV
                     RVVLYIKSFEDYKVFALNDPFRIVMDIQGNPKADEYYAVKKDTEDYKYEASLPEDQVS
                     SLSEALGLKIRTVVIDAGHGGHDPGAIGPSGLKEKQVTLNIAKALKEKLDKDGKKYGI
                     TNVYLTRSDDRFIPLEERTGIAKKYGADLFISIHCNAARDKQAYGTETYYLSLTKDQR
                     SLAVAARENATTSISRGEMSKVLKQYLLSAKIDESSRLAGHVQNSVVRNMSSKYQPVQ
                     NKGVKRAPFVVLIGADIPSILVETAFITNPRDEKRFKSEDYIDEMADGIFNGIVKFSS
                     EVQTASLK"
     gene            complement(2538..>3632)
                     /locus_tag="VFJ67_05335"
     CDS             complement(2538..>3632)
                     /locus_tag="VFJ67_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET7289843.1"
                     /translation="PRLKNLGRLVGDKHIIVCIGPGGVGKTTISSAVALESASMGKKT
                     LALTVDPSRRLAQSLGLSAGVSEGKVSAKRMKDAGYSGKGELTVSILDVKKTFDDLIR
                     KVSRSQDAAARILANNYYTSVADSIAGAHEFMAMEALYSSYTEGSYDLIVVDTPPSEH
                     LTDFLSAPLRLSSILDSQGFRSFFLMDRLSFGLTRLVTSVSLRFIQRIVGLEVIHDMW
                     EFFSEFESVSEDIKTRAARTYDLLKSPEASFLIVTNLSEAAIENTLEWERDLISANHG
                     VGAVVVNRTSRNRDLPALKGSREPGFFGSPELRENLERLYKDYKREADAEKKAFKKLG
                     SGFPCIPVPEIDTEVAGLRDLHNLREYLFG"
BASE COUNT          922 a          960 c          991 g          759 t
ORIGIN      
        1 gtgaataccg tcaggccggg gctgagcttg cgtgccacct cgaccgcctg ctccacgttc
       61 agatgggccg ggtggggctt atacctgagc gcgctgatga tgaggacctc caggtcccgg
      121 agcttgtccc acgagccttc ggggatgccg ctgcagtcgg tcagataggc cataccccct
      181 atcctgtagc cgagaatggt ccattcggcg tgatagatat caactggtat gatctccact
      241 ccatcgaaat cgaatttccc tgagacaggc gttaaggtaa ggttgggctt tccgcccgcc
      301 gactctacgc cgtcgaatat gtatttgaaa ttatttctaa tattgtcaac ggtttgagcg
      361 ttcccgaaac acctgatcga ggtcttgttg acgaagttat agaccctgag ctcgtctatg
      421 ccgtgagtat ggtcggcgtg ggagtgcgta tagaggacgg cgtcgagctt cgtgatcccg
      481 tgcctgagcg cctgacatct caggtccgtg gacgtatcga tgagtatatt cctgcccgcg
      541 gcctccacga atactgagca tcttgtcctt ttattccggg ggcttgccga cgtgcagacg
      601 ggacagttgc agccgatgat cggcacgccc gtagaggttg cggaacccag tatagttacc
      661 ttcaccgtta gaaatttatc atcaagaatc tatgattaca aaagatttac cctttataat
      721 gaaagaaaat ccggtaaaat cttaaagtag ttcataaaat accatttaca aatgaccacg
      781 aaattaatat aataatacag taaagtgagc acaaaaccgc gatttgccgc aatcgtagcc
      841 tctttcctcc tcatcaccct gatatcatcc aattcctatg ccgcggacaa gggggaggat
      901 ctgtacaaag agacgttcca gctctacaaa tccctcgccg gaagccagga aaagataagc
      961 gataaggaga tttgggatac aatagcgagg gcgttctaca gtatctacat caactatccg
     1021 cagagcgaca aggccccgaa cgccctcttt atctcaggga aaatgtatga ggagatgggg
     1081 gaaaggttcg gctccgggga cgacctagag accgctgtcg attattcgag gcttttcgtg
     1141 aagaaatacc ctgaaagcag tctcgcggac gatgctcaac tccgggtcgc caggatcgtc
     1201 gagaaagaca gcaggagcga cgcttacctc gaatacgaaa aaatcacgac ggatttcccg
     1261 aacggcgata tgaacacgat cgcgaagaac aagaccgccg agctctcgcc gttcaaaccg
     1321 gctgtgagaa aggccgatac gggcgacgag ccggcgcagc cggattcggc tgcggatagc
     1381 aagagcggcc tcgtgagcgt gagccagata cggcactggt cgaccgacaa cttcacgagg
     1441 gtagtgatcc acgtcgataa ggaaacaccg tttaaatcca tgttcctcaa ggaagacaag
     1501 gcgaacggca agccccccag acttctcgtc gacatatacg gcaccaaggt ggacaagaaa
     1561 ctttacgtcg agccgataac gaaggggctt ctcgaagaga tcaggttcgc caggaacacg
     1621 aaggacagcg tgagggtggt gctttacata aagagcttcg aggactataa ggtatttgcg
     1681 ctgaacgacc cgttcaggat cgtcatggac atacagggga atccgaaagc ggatgagtat
     1741 tacgcggtta aaaaggatac ggaagattat aaatacgagg cttcgctgcc cgaagaccag
     1801 gtttcgagct tatccgaagc gctcggactc aagataagaa cggtcgttat cgacgcgggg
     1861 cacggaggac acgacccggg agcgataggc ccttcgggac tcaaggaaaa gcaggtaaca
     1921 ctcaacatag ccaaagctct taaggaaaag ctcgacaagg acgggaagaa gtacggaatt
     1981 acgaacgtct acctcacgag gagcgacgac aggttcatac cgctcgagga aaggacgggt
     2041 atcgcaaaga agtacggggc cgatctgttc atctccatac attgcaacgc ggccagggac
     2101 aagcaggcct atggaacgga aacgtactat ctcagcctga ccaaggacca gagatctctc
     2161 gccgtcgccg cccgtgagaa cgcgactacg agcatctccc gcggcgagat gtcgaaggtg
     2221 ctgaaacaat acctcttaag cgcgaagatc gacgagtcat cgagactcgc cgggcacgtg
     2281 caaaattccg tcgtcagaaa tatgtcgtca aaatatcagc cggtacagaa caaaggcgta
     2341 aagagggctc cgttcgtcgt tctcatcggg gccgacattc ccagcatact cgtcgagacc
     2401 gctttcatca cgaacccgag ggacgagaag aggttcaagt cggaagatta tatcgacgag
     2461 atggcggacg gcatattcaa cgggatagtc aaattctctt ccgaagtaca gaccgcgtcg
     2521 ctcaagtaac tcgcgcgtca gccgaaaagg tattccctca gattgtgaag gtctctcaag
     2581 cccgccactt ccgtatcgat ctcgggaacc gggatacacg ggaatcccga gccgagcttt
     2641 ttaaaggcct ttttttcggc atccgcttcc cgcttataat ccttgtaaag tcgttctagg
     2701 ttctctctga gctcggggct gccgaaaaat ccgggttccc gagaaccctt tagagccgga
     2761 aggtcgcggt tccggctggt tctgttcacg accaccgctc ctaccccatg gttcgcggaa
     2821 atcaaatccc tttcccactc gagtgtgttc tctatcgcgg cttcgctcag gttggttact
     2881 atcagaaatg acgcttcggg agacttgagg aggtcgtagg tcctggcggc gcgcgttttt
     2941 atatcctcgc tcaccgactc gaattcggaa aaaaattccc acatatcgtg gatgacttcg
     3001 agtcccacga tcctctgtat aaacctgagc gatacggaag tgacgagccg tgtgaggccg
     3061 aagctgagcc tgtccataag gaaaaacgac ctgaacccct gcgagtcgag aatggagctc
     3121 agcctgagcg gagcggagag aaagtcggtg aggtgctcgg agggaggagt atcgacgacg
     3181 atcagatcgt agctgccttc ggtgtatgac gaatagaggg cttccatcgc catgaactcg
     3241 tgagcgccgg cgatagaatc ggcaacgctc gtatagtaat tattagcgag aatcctcgcc
     3301 gccgcgtcct gagaccggga gaccttcctt atgagatcgt cgaacgtctt cttcacgtcg
     3361 aggatgctta ctgtaagctc gcccttcccg ctgtatcccg cgtccttcat gcgcttcgcg
     3421 gagaccttgc cttcggatac tcccgcagag agcccgaggg actgcgcaag cctcctcgaa
     3481 gggtcgaccg taagagccag tgtctttttc cccatcgagg ctgactcgag ggcgaccgcc
     3541 gaggaaatgg tcgtcttgcc cactcctccc ggaccgatgc agacgatgat atgtttgtcc
     3601 ccgacgagcc gtccgagatt cttcaaccgc gg
//
LOCUS       DASSJH010000247         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1359465, whole genome shotgun sequence.
ACCESSION   DASSJH010000247 DASSJH010000000
VERSION     DASSJH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359465"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFJ67_05340"
     CDS             <1..505
                     /locus_tag="VFJ67_05340"
                     /inference="COORDINATES: protein motif:HMM:NF023298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HET7289844.1"
                     /translation="ACLNNCPVYRKVGGHSYGWVYSGPIGAIINPQLLGIDKAPELPF
                     ASSLCGACRDVCPVRIDFPKVLLELRHEVRESGAEKGKGAEPFFERLSFRLWRTIVTH
                     EKFYSILLSLAYRLQTPFRKNDALRSLPYPFSRWTRDRDFPAVSETPFRERWKKMKRR
                     GGEGGML"
     gene            460..735
                     /locus_tag="VFJ67_05345"
     CDS             460..735
                     /locus_tag="VFJ67_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289845.1"
                     /translation="MEENEKKGRGGGYAMSGRSREAILGNIRRGLYGAEHTDDGNTAL
                     DVHTISEKAAEIQEKYLIKNDSNLLEQLVKELYNVNTEVYMAGTEDE"
     gene            820..1197
                     /locus_tag="VFJ67_05350"
     CDS             820..1197
                     /locus_tag="VFJ67_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HET7289846.1"
                     /translation="MVGILEGEGLTHITSTDKNEMARAGMGITGADYAIADTGTLVLL
                     TDGSKPRSVSLLPPVHLAIVKESNIVCNINELFIILKDKLDAGEPVPSCVTFITGPSR
                     TADIELNLTLGVHGPKELHVIIT"
     gene            complement(1209..1712)
                     /locus_tag="VFJ67_05355"
     CDS             complement(1209..1712)
                     /locus_tag="VFJ67_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HET7289847.1"
                     /translation="MANKKFLIFAAATLFGGAAIISCNKTAGTGAMDDAESNPVKHGE
                     YIVKIAGCNDCHTQGYPEADGNVPVDEWLTGSPLGYRGPWGTTYAINLRLFMQGLNQD
                     EWITYARSARSRPPMPWYVLRDLSDSDLVAIYKFVRYLGPAGDPMPAYVPPGKEPTGP
                     YVEFPSD"
     gene            complement(1844..2563)
                     /locus_tag="VFJ67_05360"
     CDS             complement(1844..2563)
                     /locus_tag="VFJ67_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007245137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7289848.1"
                     /translation="MKLKGKKALVTGGARGIGKGVAAAYLKEGASVIICGRNELILKA
                     ACNDLGKLGPVDYIVADISHRDDVARLAEEIGERWVSLDVLVNNASILGERKPIIDYP
                     EDVWEEVVDINLNAQFYVTKALLPLLMKSENGSIINVSSSVGRKGKKEWGAYAASKFG
                     VEALTQVLSDELGENGPRVNSVNPGGTRTGMRADAYPDEDPMSLPSPEDISPVFVYLA
                     SDESKGVTGREFNARDWIGAK"
     gene            complement(2725..>3212)
                     /locus_tag="VFJ67_05365"
     CDS             complement(2725..>3212)
                     /locus_tag="VFJ67_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289849.1"
                     /translation="GFGEIEEIKFTFNVHKGDVEARRSWVWRTKEDIVTFSGGGTDKP
                     VTYSRKEITADSPDSLREIDAHFINDQYLLLFPFHLVWDSGMKVELAGENVKFPMGKG
                     SGRMVSVIYPPTGGYTPGDRYDLFLDDNDMIVEWIYRRGGSETPTVTARCGKTITAWA
                     P"
BASE COUNT          692 a          839 c          929 g          752 t
ORIGIN      
        1 ggcgtgtctc aacaactgcc ccgtttacag aaaggtgggc gggcactcgt acgggtgggt
       61 gtattcggga cccataggcg cgataatcaa cccgcagctc ctggggatag acaaggctcc
      121 cgagctgccg ttcgcgtcat cgttatgcgg ggcgtgcagg gacgtgtgcc cggtcaggat
      181 agatttcccg aaggtgctgc tggaactaag gcacgaggtg agggaatccg gggcggagaa
      241 agggaaggga gcggagcctt tcttcgagcg gctctctttc aggctatgga gaacgatagt
      301 cacgcacgaa aagttctact cgatcctctt atcgctcgct taccgtcttc agacgccttt
      361 caggaagaat gacgccctga gatcgctgcc ttacccgttt tcccgatgga cccgggacag
      421 ggatttcccg gcggtttcgg aaactccttt cagggagagg tggaagaaaa tgaaaagaag
      481 gggaggggag gggggtatgc tatgagcggg agatcgaggg aagcaattct ggggaatata
      541 agaaggggct tgtacggtgc tgagcataca gacgacggta atactgcttt ggacgttcac
      601 acgatatcgg agaaagcggc cgaaattcag gaaaaatatc ttataaaaaa tgatagcaac
      661 ttgttagaac aattagttaa agagctttat aacgtcaata ccgaggttta catggccggg
      721 actgaagatg aatgaaggga gtatgtaatc gggcttgtac gccggaaggg tatcgaatcg
      781 ttcgcggcgt gggagccgga cttcctgagc tcccccggtt tggtcggcat tctggagggg
      841 gaagggctca cgcatataac atccaccgat aaaaatgaga tggcgcgagc cgggatgggc
      901 ataaccgggg ccgattacgc gatcgccgac acggggactc tggtcctgct cacggacggg
      961 agcaaaccga ggagcgtatc gctccttcct cctgtgcatc tggcgatagt taaagagtca
     1021 aatatagtct gtaatatcaa tgagttattt atcatactta aagacaagct cgatgccgga
     1081 gagcccgtcc cgagctgtgt gacttttatc acagggccga gcaggacggc cgacatagag
     1141 ctaaacctta cgctcggcgt acacggccct aaagagctcc atgtaataat cacctaaaac
     1201 atcggtaatc agtccgacgg gaactcgacg tagggccctg tgggctcctt gccgggtggg
     1261 acgtaagcgg gcataggatc gcccgcgggg ccgaggtatc tcacgaattt gtatatcgct
     1321 acaaggtccg aatccgagag gtcccgcagc acgtaccagg gcatgggcgg tctcgaccgt
     1381 gcggatctcg catacgtgat ccattcgtcc tggttaaggc cctgcatgaa gagcctgaga
     1441 tttatcgcgt atgtggttcc ccatggtccc cggtatccga gggggcttcc cgtgagccat
     1501 tcgtcgaccg gcacgttacc gtcggcttcc gggtatccct gagtgtggca gtcgttacag
     1561 ccggcgattt tcactatgta ttcaccgtgc ttgacgggat tggattctgc gtcatccatg
     1621 gcgcccgtgc cggctgtctt gttgcaggaa ataatcgccg cgccgccgaa taaagtcgcg
     1681 gctgcaaata tgagaaactt tttattcgcc atctcgccat cctccgaaaa cagaattcag
     1741 ttacgagtcc actattatag gcagactcag aatcgaacgc aatggcaagg aagaagttgc
     1801 tgatccggca atgcttacga tttgagagag tttaattccg cgattatttt gcgccgatcc
     1861 agtctctcgc gttaaattcc ctgccggtga cgcctttaga ttcgtcggac gcgagatata
     1921 cgaatacggg ggagatgtcc tcgggcgagg gtagggacat cgggtcctcg tcggggtatg
     1981 cgtctgcgcg catgcccgtg cgcgtaccgc cgggatttac ggagttcacc ctgggcccgt
     2041 tctcgcccaa ctcgtcggac aggacctgcg tcagggcttc gaccccgaac ttggacgcgg
     2101 cgtaggctcc ccattccttt tttcccttgc gccccacgct cgagctgacg tttattatag
     2161 agccgttttc tgatttcatt aacagcggca gaagggcttt tgtgacatag aactgtgcgt
     2221 tcagattgat gtccacgact tcctcccaga cgtcctcggg atagtcgatg atcggtttcc
     2281 gctcgccgag tatgctcgcg ttattcacga gcacgtcgag ggatacccat ctctctccga
     2341 tctcttcggc gaggcgcgcg acatcgtccc tgtgcgagat gtccgctacg atgtaatcga
     2401 cagggccgag ttttccgagg tcgttgcagg cggctttgag aatgagctcg ttacggccgc
     2461 atataatgac gctcgcgcct tccttgagat atgcagcggc tacgccttta ccaatgcccc
     2521 tggcaccgcc cgtgacgaga gcctttttgc ccttgagctt cattacactc aattctaccc
     2581 gttatggatt gtatatctaa gagaaaccgg ggccgggtta ctgaccggct ttgatcggct
     2641 caccggagcc tgatagtttg atttctacat ccgtgagcca gacacggaac ttgccgtccg
     2701 cgccgggatg atttaacgaa acggttatgg ggcccatgcg gtgattgtct tcccacatcg
     2761 cgcggttacg gtaggggtct ccgagcctcc gcgtctgtat atccactcga cgatcatgtc
     2821 gttatcgtcg aggaacaagt catacctgtc gccgggtgtg tagccgcctg tcgggggata
     2881 aatgacgctg accattctgc ccgagccttt gcccatgggg aattttacgt tttcgcctgc
     2941 gagctcgact ttcataccgc tgtcccagac gagatggaac gggaagagta gcaagtattg
     3001 atcgttgatg aaatgggcgt cgatctccct gagcgaatcc gggctgtctg cggttatttc
     3061 cttacggctg tacgttacgg gcttgtccgt gcccccgccc gagaaagtga cgatatcttc
     3121 cttcgtccgc catacccagg acctgcgggc ttcgacatca cccttatgga cgttgaatgt
     3181 gaatttaatt tcctcgattt ccccgaagcc gc
//
LOCUS       DASSJH010000248         3024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1359578, whole genome shotgun sequence.
ACCESSION   DASSJH010000248 DASSJH010000000
VERSION     DASSJH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359578"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3024)
                     /gene="rpoC"
                     /locus_tag="VFJ67_05370"
     CDS             complement(<1..>3024)
                     /gene="rpoC"
                     /locus_tag="VFJ67_05370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET7289850.1"
                     /translation="YKRLKHRGITCEKCGVEVISSKVRRERMAHIELSSPVAHIWFLR
                     SIPSRIGMLLDMTLKDLEKALYYESYVVLDPGGTTLKFGEVLSEDKYRKAVEEYGHSF
                     KVGMGAETIREMLKKIDLVELSDQLRHEMKNTSSPIKRARIAKRLRICEAFRKSKNRP
                     EWMILTRIPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKKLLELGAPDIIV
                     RNEKRMLQESVDALLENGRRGRPVLGHNHRPLKSLSDIIKGKQGRFRQNLLGKRVDYS
                     GRSVITVGPELKLHQCGIPKQMGLELFRPFIYQKLEKWGAAATIKIAKKLVEQESPIV
                     WDALDEVIKEHPVLLNRAPTLHRLSIQAFEPILIEDKAIQIHPLVCPAYNADFDGDQM
                     AVHVPLSVEAQTECRALVMSTNNILSPAHGKPIILPTQDIVLGIYYMTRDMAYDKGDG
                     KLFASINEVQMAYDSGEIGIHAKIKVRVAGKLYETTAGRVLLYDIMPKDIPFELVNRV
                     MTKRAIEELVDTCFRMAGGKNTVILADRMRTLGFKYSTKAGISISIDDMSIPHKKKEL
                     LQKAQDEVVKVQNQYSQGLITDGERYNKVIDIWARTSDEVAQEMMRDISYEEVMDKEG
                     NVKRGTSSNPIYMMIDSGARGSQTQVRQLAGMRGLMAKPSGEIIETPITSNFREGLTV
                     LQYFISTHGARKGLADTALKTANAGYLTRRLIDVAQDVMIKEFDCGTVEGITVSDLVE
                     GGEIIERVGERVLGRVSVDDIIDPVSGQIIVAANNEIDEQAAAKIDEAGSITEVRIRS
                     VLTCETRIGVCVLCYGRNLATGKLVNIGEAVGIIAAQSIGEPGTQLTMRTFHIGGAAS
                     QRAAESTLESTHEGSVRFNNVRTVKSKDGSHVVINRTGTLIIVDDKGYERERYPLALG
                     ARLRINEAEKVKEGQLLAEWDPYTTPFISEFEGKVGFVDLEQGITLKEQVDEVTGIYK
                     KVVIESKNLDLHPGLVITTAEGKSIQYSLPIGAIIE"
BASE COUNT          505 a          896 c          805 g          818 t
ORIGIN      
        1 ctcgatgatc gcccctatcg gtagcgagta ttgaatgctc tttccctccg cggtcgtgat
       61 tacgagaccg gggtggaggt cgaggttctt tgattctata acgaccttct tataaatacc
      121 cgtaacttcg tctacctgtt cctttagagt tataccctgc tcgaggtcaa cgaaccccac
      181 cttgccctcg aattccgaaa tgaacggtgt agtgtaaggg tcccattccg cgagaagctg
      241 tccttccttc accttctcgg cttcgtttat cctgagcctg gcgccgagcg cgagaggata
      301 acgctccctt tcgtagccct tgtcgtcgac gattatgagc gtcccggtac ggttgattac
      361 gacgtggctc ccgtccttgc tttttacggt cctgacgttg ttgaacctga ccgagccctc
      421 gtgcgtcgac tctaacgtgc tttcggccgc cctctggctc gcggcgcctc cgatgtggaa
      481 cgtcctcatc gtgagctgtg tacccggctc cccgatcgac tgggcggcta tgatgccgac
      541 cgcctctccg atgttgacga gcttacccgt ggcgaggttc ctgccgtaac agagaacgca
      601 cacgccgatg cgggtctcgc aggtcagcac ggaccttatc ctgacctccg tgatgctgcc
      661 tgcctcgtcg attttggccg cggcctgctc gtcgatctcg ttgtttgctg cgactattat
      721 ctgaccgctt acggggtcta tgatgtcgtc gacggacacc cttccgagca ctctttcgcc
      781 gaccctctct attatctcgc cgccttcgac gaggtcgctc acggtgatgc cttccaccgt
      841 gccgcagtca aattctttta tcattacgtc ctgggctacg tcgatcagtc tcctggtgag
      901 gtaacctgcg ttcgcggtct tgagcgccgt gtcggcaagt cccttcctcg cgccgtgcgt
      961 cgagatgaag tactggagaa ccgtcagccc ttctctgaag ttggacgtga tcggcgtctc
     1021 gatgatctca cccgaaggct tggccatcag ccccctcatg cccgcaagct ggcggacctg
     1081 ggtctggctg cctctcgctc ccgaatctat catcatgtat atggggttcg agctggtacc
     1141 gcgttttacg ttcccttcct tgtccatgac ttcttcgtat gaaatatctc tcatcatttc
     1201 ctgggcgact tcgtcgctcg tacgcgccca tatgtcaatt accttgttgt acctctcgcc
     1261 gtccgtgata agaccctgtg aatactggtt ctggaccttg acgacctcgt cctgcgcttt
     1321 ctggagcagc tccttcttct tgtgcggtat gctcatgtcg tcgatcgaga tcgagatccc
     1381 agccttggtc gaatacttga acccgagcgt cctcatcctg tcggcgagga tcacagtgtt
     1441 ctttccgccg gccatcctga agcaggtatc gaccagctcc tcgattgccc tcttggtcat
     1501 gaccctgttg acgagctcga agggtatgtc cttcggcata atgtcgtaga ggagcacgcg
     1561 tccggccgtg gtttcgtaga gcttgccggc gacgcgtacc tttatcttcg cgtgtatacc
     1621 tatttccccc gaatcgtagg ccatctggac ttcgtttata ctcgcgaaga gcttgccgtc
     1681 ccccttgtcg taggccatgt ccctggtcat gtagtaaatg cctaagacta tgtcctgtgt
     1741 cgggaggatt atcggcttcc cgtgcgcggg ggacaatatg ttgtttgtcg acataacgag
     1801 cgccctgcac tccgtctgag cttcgaccga cagagggacg tgaacggcca tctggtcgcc
     1861 gtcgaagtcc gcgttataag cgggacagac gagcgggtgt atctgaatgg ccttatcctc
     1921 gatgagtata ggctcgaacg cctgtatgct gaggcggtgt agcgtcggag cgcggttgag
     1981 cagcaccggg tgctccttta tcacctcgtc gagggcgtcc catactatcg gggattcctg
     2041 ctctacgagc ttcttcgcga tttttatagt cgccgcggct ccccattttt cgagcttttg
     2101 gtatatgaac ggcctgaaaa gctcgaggcc catctgcttc ggtatgccgc actggtgtag
     2161 cttcagctcg ggacctaccg tgatcaccga acggcccgaa tagtccacgc gttttccgag
     2221 gaggttctgt ctgaaccttc cctgcttgcc cttgataata tcggagaggc tcttgagcgg
     2281 cctgtggttg tgcccgagca cgggtctccc tctccgcccg ttctcaagca gcgcgtccac
     2341 cgattcctgg agcatcctct tctcgtttct cacgataatg tcgggtgcgc ccagctcgag
     2401 gagcttcttg agacggttgt tcctgtttat gacgcgtctg tagaggtcgt tcaagtcgga
     2461 tgtcgcgaac ctgccgccgt ccagaggcac aagcggcctg aggtcgggag gaagaacggg
     2521 tatcctcgtg aggatcatcc attccgggcg gttcttcgat ttcctgaagg cctcgcaaat
     2581 cctgagtctc ttcgctatcc tcgccctttt tatgggtgag gaggtgttct tcatttcgtg
     2641 cctgagctgg tcggagagct cgacgaggtc tatcttcttg agcatctccc ttatggtctc
     2701 ggcacccatg ccgaccttga agctgtggcc gtactcttcg acggcctttc tgtatttgtc
     2761 ctcgctcagg acttccccga atttaagtgt cgtgcctcct gggtcgagga ctacgtacga
     2821 ttcgtagtaa agcgcctttt cgaggtcttt gagcgtcata tcgagaagca tgccgatcct
     2881 cgaaggaatg ctcctcagga accatatatg ggctacgggg gacgaaagct ctatgtgagc
     2941 catcctctcc cttctgacct tggatgaaat gacctcgacg ccgcatttct cacaggttat
     3001 gcccctgtgc ttgagtctct tgta
//
LOCUS       DASSJH010000249         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1359725, whole genome shotgun sequence.
ACCESSION   DASSJH010000249 DASSJH010000000
VERSION     DASSJH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359725"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..863)
                     /gene="coaE"
                     /locus_tag="VFJ67_05375"
     CDS             complement(255..863)
                     /gene="coaE"
                     /locus_tag="VFJ67_05375"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288980.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7289851.1"
                     /translation="MYGITGNIASGKSSVARMFEELGARIIDADDVARAVVEPGEPAW
                     KDIADEFGKEVLEPGGRINRKKLAEIVFSDEARRGKLNDITHPRIKERIKGLLREYEK
                     GGAQVVMIEATLIVERGGLRSLIDGLIVVTADEETQIERLVREKGYTRDEAVSRLGAQ
                     MPAGEKVKYGDYVIDNSGPLEDTRRRVNEVWEAICSPGNRPA"
     gene            1015..1878
                     /locus_tag="VFJ67_05380"
     CDS             1015..1878
                     /locus_tag="VFJ67_05380"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HET7289852.1"
                     /translation="MSKKPEDIKTVGVVGAGTMGSGIAQVVASSGKNVVLVDVTDAAL
                     ARGVKAIEGSLGRLVKKETISEEESKSIVSRIKTTTKYEDFKDVDLIIEAAFEDMKVK
                     KEIYKKLDESTSPDVVLATNTSSLPIVEIAVGTGRPDKVVGMHFFNPAPVMKLVEIIK
                     TLTTSDETADFAFDFATALGKEPVRTKDIPGFIVNRILIPMLNEAVFAYSDGVGTPED
                     IDKAMKLGTNQPIGPLALIDLIGLDVTIDVMDVFYKEFQDSKYRAAPLLRQMVRAGWL
                     GRKSGKGFFQY"
     gene            complement(1963..>2524)
                     /locus_tag="VFJ67_05385"
     CDS             complement(1963..>2524)
                     /locus_tag="VFJ67_05385"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET7289853.1"
                     /translation="KFTLDNVGKSAAVFNPEKLQWLNGWYIRNKPPEEIARLVLPILA
                     GKGIKAELDEKLVMIVKELGQRAKTLLDIADSLGYFYADEIEYDEKAAGKFLTPEMLD
                     VLKELIEKLTQASSFTSDIIQSVFEGIMSERGLKLGTIAQPVRVALTGGTVSPGIFEV
                     MVILGKDEVLKRLNKAIGYVSGRTPA"
BASE COUNT          541 a          705 c          643 g          635 t
ORIGIN      
        1 ccggcttcgg cctctctttc cttttcttcg agtacggcaa ttttgtcctt cattatcggc
       61 tcctttgaag gttcttgaga atccaaaaaa atactacgta aaaacctaat tggcaagttt
      121 acgcgccggg cggtgtatcc gtatctattg cccggaaata taaggaattt tatcgggggt
      181 ttccgtcggg ccggataggg cggatgcggt gcgaatttaa taccggatcc cgtgtggttc
      241 aggtttatta gccgtcaggc gggcctgttc cccggggagc aaatggcctc ccagacttca
      301 ttcacgcgtc tccgcgtatc ttctagaggc cccgagttgt cgatgacgta gtctccgtat
      361 ttgaccttct ccccggctgg catctgcgcc ccgagtctcg agaccgcctc gtccctcgta
      421 taacccttct cacggacgag cctctctatt tgtgtctctt cgtctgcagt cactacgatg
      481 agcccgtcta tcaggctcct gagtcccccc ctttccacga tcagcgtagc ctcgatcatc
      541 acaacttgtg ctcctccctt ctcgtattct ctaaggagcc ccttgatcct ttcctttatc
      601 ctcggatgcg tgatgtcgtt gagctttccc ctcctggctt cgtcgctgaa gactatctcc
      661 gcgagctttt tcctgtttat cctccctcct ggctcgagca cctctttccc gaattcgtct
      721 gcaatatctt tccacgcggg ctctcccggc tctacaaccg ccctcgctac atcgtctgcg
      781 tcgataatcc tcgcgcccag ctcctcgaac atacgtgcga cggagctttt cccggaggct
      841 atgtttcccg taataccgta caccttcacc ggggtatttt aattcggcgg caatactctt
      901 tcaaatcggg cttctttgtg tgtgcgatac gctcgatcgg gagacttgat aatattatca
      961 gttgggtata ataatccctt gatttcgcaa ctgcgaatag aaaaggaggc cgtcatgtcc
     1021 aaaaaaccgg aggatatcaa gacagtagga gtcgtaggag cgggaactat gggctcgggg
     1081 atcgctcagg tggtcgcgtc gagcgggaag aatgtggtgt tggtggacgt taccgatgct
     1141 gcgctcgcaa ggggcgtcaa ggcaatagag ggcagcctcg ggcggctcgt caagaaggag
     1201 accataagcg aggaagagag taaatccata gtatccagaa ttaaaacgac cacaaaatat
     1261 gaggatttca aggacgttga cctcatcata gaggccgcct tcgaggacat gaaggtcaaa
     1321 aaggagatct acaagaaact cgacgagtcg acatcgcccg acgtggtgct cgcgacgaac
     1381 acctcgtccc tcccaatcgt tgagatagcc gtcgggaccg ggaggccgga taaggtcgtg
     1441 gggatgcact tcttcaaccc ggcgcctgtt atgaagctcg tcgagataat aaagacgctc
     1501 acgacttcgg acgagacggc ggatttcgcg ttcgattttg caactgctct cggaaaggag
     1561 cccgtcagga cgaaggacat ccccgggttc atagtgaaca ggatactgat acccatgctt
     1621 aatgaggcgg tgtttgcata ctcagacggc gtcggcacgc ctgaagacat agacaaggcc
     1681 atgaagctcg gcacgaacca gccgataggc ccgctcgcgc tcatagacct catcggactc
     1741 gacgtcacca tagacgtcat ggacgtattc tacaaggagt tccaggattc gaagtacagg
     1801 gccgcgccgt tattgagaca aatggtcagg gcagggtggc tcggacggaa atccggaaag
     1861 ggcttcttcc agtattaata atcggcggta aatcggaggc gtgttttaaa tacgttcggt
     1921 cagtcccgta caggttgacc ggaaattcca ttgtttttta tttcaggctg gtgtacggcc
     1981 ggatacatac ccgatagcct tattgagtct cttcaatacc tcgtccttcc cgagtatcac
     2041 catcacctcg aatatgcctg ggctcaccgt cccccccgtg agcgcaaccc tcacgggctg
     2101 ggctatggtc ccgagcttga gtcccctctc actcattatg ccctcgaaca cactttgtat
     2161 aatgtctgag gtaaaagagg aagcctgcgt aagtttttcg atcagctctt tcaggacgtc
     2221 gagcatttcg ggcgtgagga acttgcccgc cgccttctcg tcgtattcga tctcgtcagc
     2281 gtagaagtat ccgagagaat ccgctatatc gaggagcgtc ttcgccctct gtccgagctc
     2341 ctttacgatc attacgagct tttcgtcgag ctcggccttg attccctttc cggcaagtat
     2401 cggcagcact agcctcgcta tctcttccgg gggcttattc cgaatatacc agccgtttaa
     2461 ccactggagc ttttccgggt tgaacacggc cgccgacttc cccacgttat cgagagtgaa
     2521 cttc
//
LOCUS       DASSJH010000250         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_495137, whole genome shotgun sequence.
ACCESSION   DASSJH010000250 DASSJH010000000
VERSION     DASSJH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495137"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFJ67_05390"
     CDS             <1..614
                     /locus_tag="VFJ67_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7289854.1"
                     /translation="YVESVRSFIDGTAPALTIKREVPKIPVYIAGLTKGAAKLAGEVA
                     DGIMPYLSTPEYIKTLAGYVAEGAKAAGRDPKDVDITLGIPSVVSDDLELARKSAKRG
                     LSGYLRFPFYQRLMTNNGYGEAIDKIKAGTDPMEVFTDEMLDSVALVGPAERCRARLE
                     EYRKAGVALPIIVPSPAGKQSNVEVMKITVAAYGGDSAISRSS"
     gene            complement(806..958)
                     /locus_tag="VFJ67_05395"
     CDS             complement(806..958)
                     /locus_tag="VFJ67_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289855.1"
                     /translation="MGGNWNTSAGHFSARAPTGFARLFGGCAEFRKIDLAAAINRLRT
                     LEIEQK"
     gene            1276..2283
                     /locus_tag="VFJ67_05400"
     CDS             1276..2283
                     /locus_tag="VFJ67_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET7289856.1"
                     /translation="MDRRDFLKFLAMGGTGAALLKGDFAFAGLDMDLQGDANADNTIN
                     TLFDSWSSQVMFQRKPYRYPYGVGHPQVTKKRLNTVLGAIKSYGLSTGVKWNVRYTDS
                     RIKPESLENVDVYVSLTRYINQPNPPPVGTGFSYVDSELAALEDFVSQGGGILLMTDH
                     GATEEIPNWTENDAALASVFGITLRNIFVTHTDPEHPDRPEYMVMEIDPDLHEDLKYI
                     ANQVAHVSAHDSCIMLPPADFTPLLKFPKGSTAYDIAVKHYIDLPNHYFSILVPYGAG
                     NVIVVGNSGIVGDYGSPDPAPGLITMENNLNFFLNCVTYLAGHSCIPDPGQGPCDFFT
                     L"
BASE COUNT          586 a          576 c          644 g          585 t
ORIGIN      
        1 cctacgtcga gtcggtgagg tcttttatcg acggtacggc tcccgcgctt acgataaagc
       61 gggaggtccc gaagattccc gtctatatcg cgggtctcac gaaaggtgct gcgaagctag
      121 cgggcgaggt ggccgacggg ataatgcctt acctctcgac gcccgagtac ataaagactc
      181 tcgcgggtta cgtggcggag ggcgcgaagg cggcagggag agacccgaag gacgtggaca
      241 ttacgctcgg cataccgtcc gtcgtgtcgg acgacctcga gctcgcgagg aagtcggcca
      301 aacgcgggct gtccggttat ctccggttcc cgttctacca gaggctcatg acgaacaacg
      361 gttacggcga agcgatagac aagataaagg cgggcacaga cccgatggaa gtattcactg
      421 acgagatgct cgactcggtc gcgctcgtag ggccggcgga gaggtgcagg gcgaggctcg
      481 aggagtacag aaaagcaggc gtcgcgcttc cgatcatcgt gcctagcccg gcggggaagc
      541 agtcgaacgt cgaggtgatg aagatcaccg tcgcggcgta cgggggggat agtgctatat
      601 cccgttcgtc ctgagcttag cctttcctga gcctgtcgaa gggtcgaagg gcgcatagta
      661 ggtgcggctt ccagcatcgt gcaccgaaag ttgccggaaa gatcaccggg agttcaccgg
      721 attccgggtt ttgagcggcg ggagctcttg ttcaaaatct tcaatacagc aatatccctg
      781 ctatagatta tatattgtat atccttcact tctgctcgat ctccagcgtc ctgaggcggt
      841 ttatagcggc agcgaggtct atcttgcgga actcggcgca gccgccgaac agtcgggcaa
      901 atccagtcgg tgcccgcgcg gaaaaatgcc cggctgaagt attccaatta cctcccatcc
      961 gctaacctca tacatatgta tggttcttta catgacattt aacctgtacc tttaaaccta
     1021 tattgggggc cggatcatcg atctccctga atgattggcg tgcaatctaa gattataaac
     1081 tattaagata tttaagaatt accttccggg aatgtatata ttgactttca aattccatcc
     1141 taatagaatg gcctctataa gatttaatag gcggttgtgt cgtatcgtgt catgcgctga
     1201 aaaaaagcga gggtattata acaactgtac gccgcaagaa acactaagca gattaggttt
     1261 ggagggaaaa gcacaatgga tcggagagat ttcttaaagt tccttgccat gggcggaacc
     1321 ggagccgcat tgcttaaggg cgattttgct tttgcgggcc ttgatatgga cttgcaagga
     1381 gacgccaacg ctgacaacac gataaacaca ctattcgata gctggagttc tcaggtgatg
     1441 tttcaaagga agccgtatcg ttatccttac ggcgttggtc atccgcaagt aaccaagaag
     1501 cgtctgaata cagtcctggg agctatcaag agttacgggc tttccacggg agtgaaatgg
     1561 aatgtgcgtt atactgattc ccggataaaa ccggagagcc tggagaatgt ggatgtgtac
     1621 gtcagcctca cccgttatat taatcagccc aacccgcccc cggtcgggac aggtttttca
     1681 tatgtagatt ccgagctcgc ggcactggag gatttcgtca gtcagggcgg agggattctt
     1741 cttatgacag accacggcgc aactgaagaa atcccaaact ggacagagaa cgatgccgct
     1801 cttgccagcg tgtttggaat taccctcagg aacatctttg tcacccacac tgatccggaa
     1861 catccggacc gacctgagta tatggtgatg gagattgatc cggatttgca tgaagatctc
     1921 aagtatattg caaaccaggt cgcgcacgtt tccgctcatg attcttgcat aatgctgccg
     1981 cctgctgatt tcactcctct attaaagttt cccaaagggt ctaccgctta tgatattgct
     2041 gtaaaacatt atatcgactt gccgaatcac tatttctcga tattggttcc ttatggcgcg
     2101 ggtaatgtta tcgttgtggg caattcggga atagtaggag attatggatc cccggatccg
     2161 gctccggggc tcataactat ggagaacaac ctgaacttct ttctaaattg cgttacatac
     2221 ctggcaggac acagttgtat tccggacccc ggtcagggac catgtgactt ttttactttg
     2281 tagtaagtac tcgttaagaa gagtcaaaca gctcgcatat ttggtgaaat cggccgtatt
     2341 atcgacatct ttgattcccg aggcgagata ttccataaag ttttacccct c
//
LOCUS       DASSJH010000251         4320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1139754, whole genome shotgun sequence.
ACCESSION   DASSJH010000251 DASSJH010000000
VERSION     DASSJH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139754"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFJ67_05405"
     CDS             complement(<1..422)
                     /locus_tag="VFJ67_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HET7289857.1"
                     /translation="MKHLAFINKAVTDYFTFWIILFSVVTFLYPAPFAGLTGLIVPVL
                     GIIMFGMGMTLTVEDFRRIAARPRDVAIGAAAQYGIMPVTGFILVKVFQLDPMLATGV
                     VLVGSCPGGTASNVITYLAKGDVALSVAMTSVTTLMAPL"
     gene            646..1026
                     /locus_tag="VFJ67_05410"
     CDS             646..1026
                     /locus_tag="VFJ67_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7289858.1"
                     /translation="MARENEKVIERLIKCINDRKVEVMDELFHDDAVMDWPQSGEKIV
                     GAANRRAIYGAFPALPTITPRRMVSAGDLVVAEASLDYGGPSPYKAVFIFEFRNGKIA
                     KETAYWSEPFPAPDWRSQWVEKMD"
     gene            1166..2872
                     /locus_tag="VFJ67_05415"
     CDS             1166..2872
                     /locus_tag="VFJ67_05415"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7289859.1"
                     /translation="MRYSQYFIPTLKEDPADAEVVSQKLMIRAGMIRKVAAGIYNYLP
                     LGLRCISKVEQIVREEMNRAGAIELLMPAVVPSELWRESGRWDYYGKELLRFKDRADR
                     EFCIGPTHEEVVTDIVRRDVRSYKQLPINLYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFDADVAGAEKSYQLMYDAYVRIFERCGLEFRAVLADTGNIGGSFSHEFMVLAPTGE
                     DVVMSCGNCGYAANLELAEIDWHEKGVKGSKEDKVVVEVHTPGLKTVEEVAEFLKVRP
                     SNLIKTMIVTADGQPVAALVRGDHELSLTKLKKALGAEIVELATAATIEEVTGGKMGF
                     SGPVGLGIRTIADRSVVGMGSSVTGANKEDYHIVNVLPGRDFELKETGDIRVASEGDK
                     CPRCKRGTLSATRGIEVGHVFMLGTKYSEAMNATFIDSDGKEKPYIMGCFGIGIGRTV
                     AAAIEQNHDENGMIFPKALAPFQVTVLPLKVKDDEIMTAAEKIYGELMNKGYEVIIDD
                     RDVGPGFKLKDAELIGIPVHIAVGPRTIKEGEAELKLRKGGVSRNVKLEEVAKEVETL
                     LS"
     gene            2890..3612
                     /gene="pyrF"
                     /locus_tag="VFJ67_05420"
     CDS             2890..3612
                     /gene="pyrF"
                     /locus_tag="VFJ67_05420"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET7289860.1"
                     /translation="MARPDLKPKERIILALDFSELRIAKFWVENLKHKIKTFKVGPIL
                     FLDAGPIGIKEFRNMGADVFLDLKFHDIPSTVEHSGRQAVRYGVKMFTVHALGGFEMM
                     KVVSDAVADEAAKHKRHKPMVLAVTVLTSHDEASLKRIGINTSTRDTVLSLASLADKA
                     GVDGLVCSGMEVEMLRKEFGERFTLVVPGIRPGGGAHDQKRVTTPAQAVASGADYLVI
                     GRAITEAENPDLVVDEIVKSIS"
     gene            complement(3979..>4320)
                     /locus_tag="VFJ67_05425"
     CDS             complement(3979..>4320)
                     /locus_tag="VFJ67_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289861.1"
                     /translation="NYKPSGVIFTKQYSEAKNIISNIAASPLLPRELKDLIYQFIDDV
                     DHNLGLIREVLIDASKELPLKYKTVDDVIKMNLIWVWNLYNDRRKDLAMKSEEILNFV
                     NEYLKIKDIMK"
BASE COUNT         1132 a         1034 c         1263 g          891 t
ORIGIN      
        1 aggggggcca tgagagtcgt cacggaagtc atcgcgacgg agagggcgac gtcccccttc
       61 gcaagatagg tgatgacgtt agacgccgtg cctccggggc aggagcccac gagcacgacc
      121 cccgttgcca gcatggggtc cagctgaaac accttgacga gaatgaaccc ggtgactggc
      181 ataattccgt actgcgcggc cgcccctatc gccacatccc tcggcctcgc ggctatcctc
      241 ctgaaatcct ccacggtgag cgtcatgccc atgccgaaca tgataatacc gaggacgggc
      301 actataagcc cggtgaggcc cgcaaaaggg gccgggtaga ggaaagttac gacagagaag
      361 aggattatcc agaacgtgaa atagtctgtt actgctttat ttataaaagc gagatgtttc
      421 aatcgggctt ctttacagga tgaattttat aagcaggcta aattacataa atagagaata
      481 ggtgtcaatc ggcgcctata cagcgttcgt atctgtaacc catttcattt gcggcccgaa
      541 tctactatag taagaataag gctcatcacc ctctgacggc ggggccgggg gtgatttatg
      601 cagctgcatc atctgtattc ataacataga caggaggcag acgtcatggc gagggaaaac
      661 gagaaggtca tcgagcgctt gatcaagtgc ataaacgaca ggaaggtgga ggtaatggac
      721 gagctattcc acgacgacgc cgtcatggac tggccgcagt cgggcgagaa gatcgtgggc
      781 gctgccaaca ggcgggctat atacggggcg ttcccggcgc tcccgacgat cacgccccgg
      841 aggatggtgt cggcaggcga cctcgtcgtt gccgaggcgt cgctcgacta cggcggcccc
      901 tctccttaca aggcagtatt catattcgaa ttcaggaacg gtaagatagc caaagaaact
      961 gcctattgga gcgagccctt tcccgcccct gactggcggt cccagtgggt cgagaagatg
     1021 gattagttaa agacggtttc catccacagg attaagttca aggctgggca tcaaataaac
     1081 ctccctttct tgattagaac catagaacac actatgtgtg tgcgtgttgc aattggacgc
     1141 ggaatcggta atttatacgg ctaacatgag atattcccaa tactttatcc ctacgctcaa
     1201 ggaagacccc gccgatgccg aggtagtgag ccagaagctc atgataaggg ccggcatgat
     1261 aaggaaggtc gcggccggca tatacaacta cctccccctc gggctgagat gcataagcaa
     1321 ggtcgagcag atagtcaggg aggagatgaa cagggcgggg gcgatagagc tgctgatgcc
     1381 cgccgtcgtg ccgtccgagc tatggaggga gagcggcagg tgggactatt acggtaagga
     1441 gctgctaagg ttcaaggacc gcgccgacag ggagttctgc atcggcccca cgcacgagga
     1501 ggtcgttacg gacatagtaa ggcgggacgt ccgctcgtac aagcagctcc cgattaacct
     1561 ttaccagata cagaccaagt tcagggacga gatacgcccc cgtttcggcg tcatgcgcgc
     1621 ccgcgagttc ataatgaagg acgcttacag cttcgatgcg gacgtggcag gagcggagaa
     1681 gtcctatcag ctcatgtacg acgcgtacgt gcgcatattc gagaggtgcg gtctcgagtt
     1741 cagggccgtg ctcgcggata cggggaacat aggcgggagc ttctcccacg agttcatggt
     1801 gctcgctccc acgggagagg acgtcgtgat gagctgcggg aattgcggat acgctgccaa
     1861 cctcgagctc gcggagatag actggcacga gaaaggggtc aagggctcga aggaggacaa
     1921 ggtagtcgtc gaggtacaca cgccgggtct gaagacggtc gaggaagtgg cggagttcct
     1981 taaagtgagg ccgtcgaacc tgataaagac gatgatagtg acggcggacg gtcagcccgt
     2041 ggccgcgctc gtgaggggag accacgaact gagcctcacg aaactgaaaa aagcgttagg
     2101 cgctgaaata gtagagctcg cgaccgccgc gacgatagaa gaagtgacgg gcggcaagat
     2161 gggattcagc ggccctgtag gcctcggcat aaggaccata gcagacaggt cagtcgtcgg
     2221 catggggagc tccgtcacgg gagccaacaa agaggactac cacatcgtaa acgtgctccc
     2281 cggaagggac ttcgagctaa aggaaacggg cgacatcagg gtagcctcgg aaggggacaa
     2341 gtgccccagg tgcaagaggg gcacgctctc cgcgacgcgc ggcatagagg tgggacacgt
     2401 cttcatgctg ggcacgaagt attcggaggc gatgaacgcc acgttcatag actccgacgg
     2461 gaaagagaag ccctacataa tggggtgctt cgggataggg ataggtagga ccgtagccgc
     2521 agcgatagag cagaaccacg acgagaacgg gatgatattc ccgaaggccc tcgcgccttt
     2581 tcaggtcacc gtgctcccgc tcaaggtgaa ggacgatgag ataatgacag ccgccgagaa
     2641 gatatacggt gagcttatga acaaaggata cgaggtcata atagacgaca gggacgtcgg
     2701 cccgggattc aagctgaagg acgccgagct gatcggcata cccgtacaca tagccgtagg
     2761 gccgaggacg attaaggaag gggaggcgga attgaaactg cgcaagggtg gcgtttcgcg
     2821 taacgtgaag ctcgaagaag tggcgaagga agtagaaacg ctcctctcat agatatataa
     2881 ttcaaccgga tggcaagacc cgacctgaaa cccaaagagc ggataatact cgcgctcgat
     2941 ttctccgagc tcaggatagc gaaattctgg gtcgagaatc tcaaacacaa gataaagaca
     3001 ttcaaggtag gcccgattct gttcctggat gcggggccga tagggataaa ggagttccgc
     3061 aacatggggg cggacgtttt cctcgacctc aagttccacg acataccttc gacggttgag
     3121 cattcaggga ggcaggcggt gcggtacggg gtaaagatgt tcacagtcca cgcgctaggc
     3181 ggattcgaga tgatgaaggt ggtctcggat gcggtcgccg acgaagccgc gaaacacaag
     3241 aggcataagc ctatggtgct ggctgtaacg gttctcacga gccacgacga ggcgagcctg
     3301 aagcgcatcg gtataaacac ttcgacgaga gatacagtgc tgagcctagc ttcactcgcg
     3361 gacaaggcgg gcgtggacgg gctcgtgtgc tcagggatgg aagtggagat gcttagaaaa
     3421 gaattcggag aaaggtttac tctggtcgtc cccggaatac gccccggcgg aggggctcac
     3481 gatcagaagc gtgtcactac ccccgcccag gcagttgcca gcggcgccga ctatctcgtc
     3541 atcggccggg cgataaccga ggcagagaat ccagacctgg ttgtggatga gattgttaaa
     3601 tctatttcgt gatttattaa atccacccta accctccttt acaaaggagg gaaaaaatca
     3661 aaggcacaac tgaacgacat agaacttaat agattccttc cccctagagg ggggggaagg
     3721 gtaggatggg ggtgatagat ttccttggtc tttttatcga aactaccaaa gactaaatcc
     3781 ccctgtgttg gtttaaccgt gggagtcact tcgacaaaga agcttattag ctttcagcca
     3841 ataccgacca attcctccga tttgtcggaa ttgttcccct ttttctaagg gggagattaa
     3901 agaggaaacc gcgcaatcac cgaggccgat aatcccggat tggttgtgga tgagattgta
     3961 gaatctatat agtgtgtctt acttcatgat atcttttatc ttcaaatatt cgtttacaaa
     4021 atttagaatt tcttcagatt tcatggcgag gtcttttctc cggtcattgt ataaattcca
     4081 tacccatata agattcattt ttatcacatc atcaactgtt ttatatttca aaggtaattc
     4141 tttggaagca tcaattaaca cttcccttat tagacctaga ttatgatcaa catcatcaat
     4201 aaattgataa atgagatctt ttaattctcg gggcaataaa ggcgaagcag ctatatttga
     4261 tatgatattt tttgcctcgc tatactgttt tgtgaatata acaccagatg gtttgtaatt
//
LOCUS       DASSJH010000252         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1139932, whole genome shotgun sequence.
ACCESSION   DASSJH010000252 DASSJH010000000
VERSION     DASSJH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139932"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VFJ67_05430"
     CDS             complement(<1..990)
                     /locus_tag="VFJ67_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET7289862.1"
                     /translation="MGEVIATLFGIIIGLGVLVYAFSVYREIKSLPDGNDKMREIAAA
                     ISEGAMVFLSREYRIIGIFVIVVFILLGIFIDWKTAVAYVGGAVCSLTAGFFGMKAAT
                     KANVRTAHAAATEGQGKALTVAFKGGNVMGLAVAGIGLLGLSVAFTLGIGLPDNPGGG
                     AGIALFAEKFAAIIGGFAMGASSVALFARIGGGIYTKGADVGADLVGKVEAGIPEDDP
                     RNPATIADNVGDNVGDVAGMGADLFESYVGAVVATIVIAASYGKNEIALMSIPIILIA
                     IGSFSSIIGAKSVEWMKDLEPQLVLRRATFYTGGIYLAVSLVVMLMVGLVDGIG"
     gene            complement(1011..1604)
                     /gene="pth"
                     /locus_tag="VFJ67_05435"
     CDS             complement(1011..1604)
                     /gene="pth"
                     /locus_tag="VFJ67_05435"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7289863.1"
                     /translation="MILVVGLGNPGSEYASTKHNLGYLAVDEIGKRAGIDLTRKKFHG
                     LYGEGTFRRDKLILLKPETYMNRSGESVSNAVNFYHIPPENIIVVHDELDLPAGTVRI
                     KPGGGSAGHKGVASIIERLGNGDFARIRIGIGKPKQKSGTVSHVLSKFSKEENEIVNE
                     SVLKAADAVLEVIEHGLRKAMNKYNVRTESTDPESQQ"
     gene            complement(1614..2267)
                     /locus_tag="VFJ67_05440"
     CDS             complement(1614..2267)
                     /locus_tag="VFJ67_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET7289864.1"
                     /translation="MAQNILEVSIRTKPGKSGAREVRKLGNIPAVLYGKGTEPVLLSV
                     NPAALKQALSTEAGENTLLEITYSDDSNEVKKLSLLREIQYDFLTSKPIHFDFQALDI
                     NKKITVDVPVKIVGKSKGVKDGGILEEILREIPVECLPKDIPNSFEIDVTELEIGHSI
                     HVSALKVSEAFDILKDDEETIVTILAPKMEVEAPAAGAEEVPEKAAEGEVKEEGGEE"
     gene            complement(2289..3230)
                     /locus_tag="VFJ67_05445"
     CDS             complement(2289..3230)
                     /locus_tag="VFJ67_05445"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET7289865.1"
                     /translation="MDNAKIFCGTSNVPLAKAVCKYLDVPLGKSEVRRFSDGEVFVEI
                     SESVRGSHVFVIQSTCPPVNEHIMELLIITDALKRASARAVTAVIPYYGYARQDRKVQ
                     PRAPISAKLVADLITIAGISRVVTVDLHAGQIQGFFNIPVDHIYATPVFMEYIKEKFS
                     GEDVVIVSPDAGGMERARSYAKILDYGLAMTDKRRPNPNEAEIMNVIGDVDGKSVILV
                     DDLVDTAGTAIQAGKALMREGAKSVTLCSTHGVLSGSAIEKIKKSDLEEIIITDTIAP
                     SEEAAGCSKIKVISISNLLGEAIKRIATGESISSLFN"
BASE COUNT          674 a         1012 c          811 g          921 t
ORIGIN      
        1 gcctatgccg tccaccaggc ctaccatgag cattacgacc agcgagaccg cgagataaat
       61 accgcccgtg tagaacgtcg cccgcctcaa aaccagctgc ggctcgaggt ccttcatcca
      121 ttcgacgctc ttagccccta ttatcgacga gaagctcccg attgcgatga ggattatcgg
      181 tatggacatg agggcgattt cgttctttcc gtagctcgcg gcgatgacga tcgtagctac
      241 gactgcgccg acgtaggatt cgaacaggtc cgctcccata cccgccacgt cgcccacgtt
      301 gtcgcctacg ttgtcggcta tagtggccgg gttcctgggg tcgtcttcag ggatcccggc
      361 ttcgaccttg ccgacgaggt cagcgcccac gtcggccccc ttcgtatata tacctccgcc
      421 gatccttgcg aacagcgcta cggagcttgc gcccatagcg aagccgccga ttatagccgc
      481 gaatttctct gcgaacagag ctatgcccgc gccgccgccg gggttatccg ggagcccgat
      541 accgagagtg aacgcaacag agagtccgag gagtccgatg cccgctacag caaggcccat
      601 cacgtttccg cccttgaaag ctactgtcag cgcttttccc tggccttccg tagccgcggc
      661 gtgtgccgtc ctgacgttgg ctttagtcgc cgccttcatg ccaaaaaatc cggcggtcag
      721 cgaacagaca gcgcccccga catacgctac ggcggtcttc caatctatga aaataccgag
      781 gagtataaat accacgatca cgaatattcc gattatccgg tattcacggc ttaagaagac
      841 catcgcgcct tcggagatcg ccgccgcgat ttccctcatt ttgtcgttgc cgtcgggaag
      901 agacttgatt tccctgtaca ctgaaaaggc gtataccaaa acacccaacc ctataattat
      961 tccaaagaga gtcgctatca cctctcccat agtcatacac ctcctctaat ttattgttga
     1021 ctctcggggt ccgtactttc ggttctgacg ttgtatttgt tcattgcttt ccggaggccg
     1081 tgctcgatca cttccagaac cgcatcagcc gcttttaaga cggattcatt cacgatttca
     1141 ttttcttcct tgctgaattt cgacagcacg tgcgaaacgg tgccggattt ctgcttgggt
     1201 ttacctatgc ctatcctgat tctggcgaag tcgccgtttc cgagcctttc tattatcgac
     1261 gcgacccctt tatggccggc gctgcccccg cccggcttga tccttaccgt ccctgcgggc
     1321 agatcgagct cgtcgtgcac gactataatg ttttcaggcg ggatgtggta gaagtttact
     1381 gcgtttgaaa ccgattcccc gctccggttc atgtatgttt ccggcttcag cagaatcagc
     1441 ttgtctctac ggaaagttcc ttccccgtac aggccgtgga acttttttct cgtcagatcg
     1501 atcccggccc ttttccctat ttcatccacg gccagataac cgaggttgtg ctttgtcgaa
     1561 gcgtattcgc ttcccgggtt gccgaggccg accactaata tcatttcgtt taattattct
     1621 tccccgcctt cttcttttac ttcgccttca gcagcttttt ccggaacctc ttccgcgccc
     1681 gccgccgggg cttctacttc catcttcgga gccaatatcg tcacgatcgt ttcttcgtcg
     1741 tctttcaata tgtcaaaggc ctcggacacc ttgagagcgc tcacgtgtat cgaatggcct
     1801 atctcaagct ccgtaacgtc tatttcgaag gagttcggaa tatctttcgg aaggcattct
     1861 acgggaattt ccctgagtat ctcttccagt atcccgccgt ctttcacgcc ctttgatttg
     1921 cctactatct tcaccgggac gtccaccgtt atcttcttgt ttatatcgag ggcctgaaaa
     1981 tcgaaatgaa tcggcttgct cgtcaggaaa tcgtattgaa tctcacggag aagcgagagc
     2041 ttcttcactt cattggaatc gtcgctgtac gtgatttcga gtagagtgtt ctcgcccgct
     2101 tccgtcgaca gggcctgttt caatgccgca gggttaactg acagcagtac cggctctgtc
     2161 cccttcccat aaaggaccgc gggtatgttc cccagcttcc tgacttccct cgccccgctt
     2221 ttcccgggct ttgttcttat cgatacttcc aggatgttct gtgccatgac gctcgctcct
     2281 tctttgtgtt aattaaacag gctgcttatg gactcgcccg tcgcaattct ttttatcgcc
     2341 tccccgagga ggttcgaaat gcttattacc tttattttac tacaaccggc cgcttcctct
     2401 gatggagcga tcgtgtcggt aattataatt tcttccaggt ctgatttctt aattttttct
     2461 attgcgctgc ccgagagcac gccgtgcgtg ctgcagagcg ttacgctttt tgccccttcc
     2521 ctcatgagag ccttccccgc ctgtatcgct gtccccgcgg tgtcaacgag gtcgtctacc
     2581 aggattaccg attttccgtc gacgtcccct attacgttca tgatctcggc ttcgttcgga
     2641 ttcgggcgtc tcttgtccgt catggcgagt ccgtagtcga ggatcttcgc gtaagacctc
     2701 gccctctcca ttcctcccgc gtctggggaa acgatgacta catcctctcc gctgaacttc
     2761 tcttttatat attccatgaa aacgggcgtt gcgtagatat gatcgaccgg gatgttgaaa
     2821 aacccctgta tctgtcccgc atgcaaatct accgtcacca ccctgcttat gcccgctatc
     2881 gtaatgaggt ccgcgaccag cttcgcggat atcggcgccc gcggctggac tttacggtcc
     2941 tgtctcgcat agccgtagta gggtatgaca gctgttacag cccttgccga agccctcttg
     3001 agagcgtccg tgataatcag cagctccata atatgctcgt tcaccggcgg gcaggtggac
     3061 tgtatgacaa atacgtggga gccgcgtacg ctttccgata tttcgacaaa cacctctccg
     3121 tcgctgaagc gcctgacctc ggacttgccg agcgggacgt ccaggtattt acaaacagct
     3181 tttgccagcg ggacgttgga agtcccgcaa aatatctttg cgttgtccat ttgatctcac
     3241 atgaaattga ttgaggacga tgtccgatat aaaaaacaag gtattataaa atagataaat
     3301 cataattcaa gttcagtttt cggtccgggc aggcaggatt gatagcgtta ccgaaagaca
     3361 aggaaatggg gatcctcctc gcgaatcggg tcgccaggct ggcgaccgcg gacggctc
//
LOCUS       DASSJH010000253         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_387273, whole genome shotgun sequence.
ACCESSION   DASSJH010000253 DASSJH010000000
VERSION     DASSJH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387273"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..379)
                     /locus_tag="VFJ67_05450"
     CDS             complement(29..379)
                     /locus_tag="VFJ67_05450"
                     /inference="COORDINATES: protein motif:HMM:NF006749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HET7289866.1"
                     /translation="MLYIIIKTVLTAAVIVGVSELGKRHTAFAAMLAALPLTSLLAMI
                     WLYTDTKDAGRIADLSTAIFWLVLPTLLFFLILPWMLRHNYNFWLSLGVSSAVMVVFY
                     LAYAALGRKLGLPL"
     gene            524..1492
                     /locus_tag="VFJ67_05455"
     CDS             524..1492
                     /locus_tag="VFJ67_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HET7289867.1"
                     /translation="MRVTSYTIQEDLHLEEETATAAALHERLKDGRPRWVDIVSPDDD
                     ELRSILTPLVHDDSIVEDFILKERNLPVAHSEQELYFEFPIIRDMASFDHEYISFLCM
                     PHLLITIHDHEIEVLNKLSHDLCKHLHLQRATIASLLFNLMAELVEKNYFLYMDQREK
                     ITKLSNRVIKGQNSIDVRTILHLKKNIGRINIMIEDQLYCINTIQIYEGVAFDIGKQR
                     RNFNNLEDEIRNGFRFLSRYENRLEDIHKHYELMLQEKTNNRLKVLTIVAAIFLPLNL
                     IAGIYGMNFEYIPTLKWDFGYHAALAFMTLVAVAMVYYFYRHGWFK"
     gene            1865..>3611
                     /locus_tag="VFJ67_05460"
     CDS             1865..>3611
                     /locus_tag="VFJ67_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7289868.1"
                     /translation="MNFERMFFSTTVLITLALTIIISVALLFFPLVGALGFEYAAVTG
                     FFLSFISVFISAELITGDGKNYSAGKRISDRVSSVLSVNLVFLIFVYVIGLLGSLIKG
                     DCFIREGTVFFLLIPVVSVFFSATLGLLAGFTLRRKGFIVGALALIAIFAYSLLELYR
                     EPSLFVYNPLFGFFPGPIYDEAIPVTDTLIAYRLITVLWGLLFLILLYLANGFGYRRV
                     GAWDFLKLIAVGIALFTAYSYEHELGISFDREYITGEILPASVETDHFIIYYDPASPE
                     ARRIKLIAGDHEWRYMELSGFLEVDSAEKIRSYIYPDSDTRKRVVGAGDTTIANPIHK
                     EIHLVYDTFPQPILKHELTHVMAGDFGTEYLRLSPKIGLLEGLAVAADWSGDVYDPHQ
                     WSKALIENGMAPDIENIAGFGFWYAPPGQSYTLMGSFSRYLIDTYGMEKFKTVYRTGD
                     FSLYGESLPELEADWKKFLAGVPAPAGIDELAKSRFGAPAIFTATCPRRVAALKAEGF
                     RYYAEDNFRKARETFEEALGYDGQDPVLLNGLAYSSYYEGDYDGAVKAAGQTGGLTEV
                     DRAALENLRGNALWQK"
BASE COUNT          870 a         1034 c          974 g          733 t
ORIGIN      
        1 attgggaccg ggtactgagc agcgtccttc agagaggcag gccgagcttt ctgcctaacg
       61 cggcgtaggc gagatagaaa acgaccatca ccgccgacga gacccctagc gacagccaga
      121 aattgtaatt atgcctcagc atccagggca gtatcaggaa gaacaggagc gtcggaagca
      181 cgagccagaa tatggccgtc gagagatcgg caattcgccc cgcgtccttc gtatccgtat
      241 agagccatat catggccaag agggacgtga gagggagcgc agccagcatg gcggcgaaag
      301 ccgtgtgcct cttgccgagc tccgataccc ccactattac ggccgccgtt aacacagtct
      361 taataatgat atataacatc gcccgcacct tccctgttca tgtaattgat attaaaatac
      421 tcccagtccg tgaagcttac aatgaccggg gcggcgggat cagaatatgc ggactcaatc
      481 gagcgcgggg ccggtaacag gtaagcgcat cggaggctga gacttgagag taacttcata
      541 cactatccaa gaggaccttc atctggaaga ggagacagcg acggcggcgg cgctccacga
      601 gaggttaaaa gacggcaggc cgagatgggt cgacatagta agcccggacg acgatgagct
      661 gaggagcatc cttacgccgc tcgtccacga cgactccata gtcgaggatt ttatactgaa
      721 ggagcgcaac ctgcccgtag cgcattcgga acaagaactc tacttcgaat ttccgattat
      781 tagggacatg gcctcgttcg atcacgaata tatatccttc ctctgcatgc ctcatctcct
      841 gataacgatt cacgaccatg agatcgaggt attgaacaag ctgtcccatg acctgtgtaa
      901 gcatctgcac ctccagaggg ccacgatagc gtccctcctc ttcaacctca tggcggagct
      961 ggtggaaaaa aattacttcc tctacatgga ccagagggag aagataacga agctcagcaa
     1021 cagggtcata aagggacaga actcgatcga cgtcaggaca atccttcatt taaagaagaa
     1081 cataggacgc ataaacatca tgatcgaaga ccagctctac tgcatcaaca caatacagat
     1141 atacgaaggg gtggcgttcg atataggcaa gcagcgtagg aatttcaaca acctggagga
     1201 cgagatacgc aacggtttca gatttttgag caggtacgag aacaggctcg aggacataca
     1261 caaacactac gagctcatgc tccaagagaa aacaaacaac aggctcaagg tgctgaccat
     1321 agtggcggcg atattcctgc ccctcaacct gatcgctggc atctacggca tgaacttcga
     1381 gtatatccca acgctcaaat gggatttcgg gtatcacgcc gcgctggcgt ttatgaccct
     1441 cgtcgcggtc gcgatggtct attatttcta ccgccacggc tggtttaagt gacgggcgca
     1501 cacaatgcgt gctcatacgc attcattccg aatcttttct gcctgacccg caagtgatcc
     1561 caccacgttc aacccttccc ggctatggct cgggtcttcg cagagacgag cgacaagctt
     1621 ccggattcgc tgtagctacg ccggatcacc cgacggatca tcgcctttgg gcgacgtgcc
     1681 gcagcagggc gaacgggttc tagattcccg attaaaacat tcgggaatga catataaaga
     1741 gtaattctcc tccagactat gaattcaaat atcatgcgcg tccatacccc cctctccccg
     1801 aacgtggtgt aaaatatagg gcagccacgg caagaattcc cggagagagt gtaaaggcaa
     1861 gagaatgaat ttcgagcgaa tgttcttctc cacaaccgta ctgataaccc ttgctctcac
     1921 aataatcatt tcggtcgccc tcctcttctt cccgctcgta ggcgcgctcg gcttcgaata
     1981 cgcggcggtc acgggttttt tcctttcatt tatctccgtt ttcatatcgg ccgagctcat
     2041 tacgggcgac ggcaagaact actccgccgg aaagcgtatt tcggacaggg tgtcgtccgt
     2101 gctatcggta aacctcgtct tcctcatatt cgtctatgta atcggactcc tgggctccct
     2161 catcaagggg gactgcttta tcagggaagg cacggtgttc ttcctcctga taccggtagt
     2221 cagcgtcttc ttctcggcca cgctcggact gctcgccggg ttcacgctca ggagaaaagg
     2281 gttcatcgtc ggagcgctcg cgctcatcgc catattcgcg tactcgctcc tcgagcttta
     2341 cagagagccc tcgctcttcg tgtataaccc gctcttcggg tttttccccg gccctatcta
     2401 cgacgaggcg atacccgtga cggatacgct catagcgtac aggctcatca cggtcctttg
     2461 gggtctcctc ttcctgatac tgctatacct cgcgaacggg ttcggctaca gacgcgttgg
     2521 cgcgtgggac ttcctgaagc tcatcgccgt cggcatcgcg ctctttacgg catactcata
     2581 tgagcacgag ctcggcatca gcttcgacag ggaatacata accggggaga tactcccggc
     2641 gtccgttgaa accgaccact tcatcatcta ctacgacccc gcctcccccg aggcccggcg
     2701 catcaagctc atagccgggg accacgaatg gaggtacatg gagctgagcg gtttcctcga
     2761 agtggactcc gcggagaaga tacgctctta catctacccc gattcggata cgcgcaagag
     2821 ggtcgttggc gcaggcgaca cgacgatagc aaacccgata cacaaggaga tccacctcgt
     2881 ttacgacacc tttccgcagc cgatactcaa acacgagctt acgcacgtga tggccgggga
     2941 cttcgggacg gagtatctcc gcttgagccc caagatcgga ctcctcgaag ggctcgccgt
     3001 cgcagccgac tggagcggcg acgtttacga cccgcaccag tggtcaaagg ccctcataga
     3061 gaacggcatg gccccggaca tagaaaacat cgccggattc gggttctggt acgcgccccc
     3121 ggggcagagc tacacgctca tgggctcctt ctcgagatac ctcatcgata cgtacggaat
     3181 ggagaaattc aagacggttt acaggacggg cgacttttcg ctttacggag agagcctgcc
     3241 cgagctcgag gccgattgga agaaattcct cgcgggcgtg cccgctcccg caggcataga
     3301 cgagctcgcg aagtcgcggt tcggcgcgcc cgccatattc acggcgacct gcccgaggag
     3361 ggtggcggcg ctcaaggccg aggggttcag gtactacgcc gaggataatt tcaggaaggc
     3421 gcgggagacc ttcgaagagg cactcggata cgacgggcag gacccggtgc tcttaaacgg
     3481 gctcgcgtac tcgagctact acgaggggga ctacgacggg gccgtaaaag cagcaggaca
     3541 aacaggcgga ctcacggagg tcgacagggc cgcgctcgag aacctcaggg gcaacgccct
     3601 ctggcaaaag g
//
LOCUS       DASSJH010000254         5923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_495744, whole genome shotgun sequence.
ACCESSION   DASSJH010000254 DASSJH010000000
VERSION     DASSJH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495744"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFJ67_05465"
     CDS             <1..681
                     /locus_tag="VFJ67_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7289869.1"
                     /translation="VSSILASSVPLSGAYATVGMGTFLAAVTQAPLTSIFLIFEMTQS
                     YQVVIPIMISSVLGSMTSRLLIGGSLESLELSKAGIHLEEEVGGRIIHLLRAKDIMRR
                     DVETIPENMTLRELIEFLPRSRFTTFPVVDKDENLVGIISIQDFRKWIFEESIKDLVV
                     VKELATLNVVTVNPEDTLYTVLKKWEKKPVEILPVIESKTSKKVLGALSRKDVIAAYN
                     DALSNKTV"
     gene            complement(730..2274)
                     /gene="pckA"
                     /locus_tag="VFJ67_05470"
     CDS             complement(730..2274)
                     /gene="pckA"
                     /locus_tag="VFJ67_05470"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET7289870.1"
                     /translation="MIGKELFDRHGIRNPGKIYYNAPVSALYEETVRRGEGEIAEGGT
                     LVAYTAPHTGRSPQDRFIVKEPGSEAEILWGPVNKPITPENFDSLYKKVTEHLEGKDL
                     FVRDLYVCAHPEYRMRVRVINEYAWHNVFVNNLFIRPGREELPHESVEFTVIAAPDVE
                     ADPETDGTRTGTFVVLNLDKRVAIIGGTRYAGEMKKSVFTVLNFLLPREGVFPMHCSA
                     NVGRDGDVALFFGLSGTGKTTLSADPVRALIGDDEHGWFDSGVFNFEGGCYAKVIKLN
                     PETEPEIYDASLKFGSVLENVEVEPETRRINFDSDRFTENTRSAYQIDSLRNIVPGGV
                     GGIPRNVFMLTYDAFGVLPPISKLTAEQAVYYFILGYTAKVAGTERGVTEPQATFSSC
                     FGAPFLPRPPLYYAEMLKLKLSDSGASVWLLNTGITGGPYGVGKRMPLPQTRALVNAA
                     VNGELDKGKFKEVPVFSLMVPESCPGVDAKLLEPRKSWADPAAYDAKAEELRARFEQE
                     FAKHKA"
     gene            2644..3135
                     /locus_tag="VFJ67_05475"
     CDS             2644..3135
                     /locus_tag="VFJ67_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET7289871.1"
                     /translation="MFKVGNKAVYPAHGVVQVKAVEAKEICGTKRNFYILQVVDSDVT
                     VMVPTDNAETVGLRPVITKRQITKIYDILKTKIRNSNHQNGSQSWNKRYREYSDKLKS
                     GDIFEVAVVLRDINHLQKDKDLSFGEKRIMDSARTLLIKEISIAKNLGEDSVTKEIEK
                     LLG"
     gene            3200..4072
                     /locus_tag="VFJ67_05480"
     CDS             3200..4072
                     /locus_tag="VFJ67_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7289872.1"
                     /translation="MANGILDIETASRLRRFYFRSPSRVRGRKAGLHKSLYKGISPDF
                     LEYKEYNRGDELRQVDWRLYGRHDRLYVKKFEDEVNLAWCILVDSSASMGYGEGVSRK
                     LRYSAGLAATLAYLLLRQGDSVGGGAFSADGLSVIPPRAGNSYIAPILEKLETLAPAG
                     MTALSEPLLKALEVFRQDASFVIISDLLTDEREIEKSLQLLRAAKKDAVIFHVLHEDE
                     VEFSFSGSLEFLDMESDEKVIVDTDAVRDGYKRRIREFTERLKVLCHEYESRYVFSPS
                     SRPYEESLIEIADK"
     gene            4084..>5923
                     /locus_tag="VFJ67_05485"
     CDS             4084..>5923
                     /locus_tag="VFJ67_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HET7289873.1"
                     /translation="MNFSFLNPLFLIGLAAVALPIIAHLISRKSGVTKKFPAVRFLIA
                     SQGDASARSRLKDLLLLLLRAWVIVLLALVFSKPALFSLAPAGASDPKSLAIIVDNSF
                     SMVYGDNFGLAREKAAELIDTLPDGSFAIVAPLVTPEGVRLSPSGDREALRDALGEIK
                     ISGSFTRNEKRLEEALGAIDGTPGGAKEVVFITDLQKNGWTGEDAGKGWLRLIDVSPG
                     PSPANRAVTSIDTAYGKDSVTLRLYVSNFSDKPEDSLLATAALGGEELSAYLDIPPGG
                     TAGKELTIPIGPVPGDKSPDAVQEISGEGSVSIPRDSLHIDDTRYFVLSRRDVLGVLI
                     VDGDPREDARLSETYYLARAAETISEITGAHLTVKDNDSFLDEKLSGYDMVFLANVGD
                     ITEASAKELSDFAARGGTVVIFLGERVRASSYNALLKDTLPAELITEEDRETRIIPGP
                     GNTFPNDVAERLSQVGVRKYIKALPSQDAQVLLSLENGDPFMIERPLGNGRVFLVTST
                     ADTSWNNFSITPVFLPVVKSLLDIPGSVNEGRRNYQVGDTVPLDIPAGAVSVEVINPS
                     GKKNALDTATAAFDGTYEPGIYTVKTSGKDLYKFAVNVDPSESNLEK"
BASE COUNT         1353 a         1684 c         1689 g         1197 t
ORIGIN      
        1 gtgagcagca tactcgcgag ctcagtgccg ctcagcgggg cttatgcaac cgtcgggatg
       61 ggaacttttc ttgccgcggt aactcaggct cctctcacat ccattttcct gatattcgaa
      121 atgacccaga gctaccaggt agtaatcccg ataatgataa gctcggtcct cggcagcatg
      181 acttcccggc ttctcattgg aggctctctc gagagcctcg agcttagtaa agccggaata
      241 caccttgagg aagaagtcgg cggacggata attcacttgc tgcgtgcgaa ggatataatg
      301 agacgtgacg tcgagacgat acccgagaac atgacgctcc gggagctgat agaatttctg
      361 cctcgaagcc gctttaccac gttcccggtc gtggacaaag atgagaatct cgtcggaata
      421 atttccatac aggatttcag gaagtggata ttcgaggaat ccataaaaga tctcgtcgtg
      481 gtaaaagagc tggccacgct caacgtggtg accgtaaacc ccgaggatac gctctatacg
      541 gtattgaaga agtgggagaa aaaacccgtc gaaatcctcc ccgtgatcga atcgaagacc
      601 tcgaaaaagg ttttaggcgc gctttcgaga aaggacgtga tcgccgctta caatgacgca
      661 ctctcaaaca aaaccgtgtg agacgaaaca actcggggac agacagcggt tacttttatg
      721 ttagagggtt cacgccttat gcttcgcgaa ctcctgctcg aacctggccc tgagctcctc
      781 agccttggcg tcgtacgctg cggggtcggc ccagctcttc cggggctcaa gaagcttcgc
      841 atctactccg ggacaggatt cgggcaccat caggctgaac acgggcactt ccttgaactt
      901 gcccttatcg agctcgccgt tcacggcggc gttcacgagc gccctcgtct gcgggagcgg
      961 cattctcttt ccgaccccgt acggcccgcc ggtgatacct gtattgagca gccatacgga
     1021 agcgccgcta tcgctcagct tcagcttcag catctcggcg taatatagag gcggtctcgg
     1081 caggaacggc gccccgaagc aggagctgaa cgtcgcctgc ggctcggtca cacccctttc
     1141 cgtcccggcc accttcgccg tgtacccgag tatgaagtaa tacaccgcct gctcggccgt
     1201 gagcttcgag atggggggca gcaccccgaa cgcgtcgtac gtgagcatga agacgttcct
     1261 gggtatcccg ccgactcctc cgggcacgat atttctgagc gagtctatct gatacgcgct
     1321 cctcgtattc tccgtgaacc tgtcgctgtc gaagtttatc ctgcgcgtct cgggctctac
     1381 ctctacgttc tcgagcacgg agccgaactt gagggacgcg tcgtatatct cgggctccgt
     1441 ctcggggttg agctttatca ccttcgcgta gcagccgccc tcgaaattga acacgccgct
     1501 gtcgaaccac ccgtgctcgt cgtcccctat gagcgcgcgc accgggtccg ccgagagcgt
     1561 cgtcttgccc gtccccgaga ggccgaagaa gagcgcgacg tccccgtccc tgcctacgtt
     1621 cgccgagcag tgcatcggga atacgccttc cctcggcagg aggaaattga gtacggtgaa
     1681 cacggacttc ttcatctcgc ccgcgtaacg ggtgccgcct atgatggcta cgcgcttgtc
     1741 gaggttgagc acgacgaacg tccccgtcct agtgccgtcc gtctcggggt cggcttcgac
     1801 gtccggcgcg gcaatcaccg tgaattctac cgactcatgg ggaagctcct cgcgcccggg
     1861 cctgataaac aggttgttga cgaacacgtt atgccacgcg tattcgttta tgacccgcac
     1921 gcgcatacgg tattccgggt gtgcgcaaac gtagaggtct ctgacgaaaa ggtctttccc
     1981 ttccaagtgt tccgtaactt ttttatagag agagtcgaag ttttcgggcg ttatcggttt
     2041 gttaacgggc ccccagagta tctccgcttc cgatcccggc tcttttacta tgaacctgtc
     2101 ttggggcgag cgtcccgtgt gcggggcggt ataagcgacg agagtaccgc cttcagcgat
     2161 ctccccctcc cctctcctta ccgtctcttc gtagagggcg gagaccggcg cgttataata
     2221 gatctttccg gggttcctga tcccgtgcct gtcgaaaagc tcttttccaa tcatgtgccg
     2281 caccggataa cacctccctc gacttttata tgcaagtagt tttcgggaaa aggtaaatgt
     2341 atatcaaccg ttgatgcatt tcaagaatcc cgaatagaga tattttaatt ctttacagtt
     2401 acttctatca taagcctccc gtctatgaga accccgggca tgacccttcc gcgcgttacc
     2461 gggcggggga aacggtgagg ttaaaatact cctccggggg cctccggagg gcactccggc
     2521 ccactcttcc ggccggaaca gtgagacgcg gcccgcccct gctcatttga cctgaagccc
     2581 gaattgtgat agattttagt cagcagcacc actcatcaca caaaggggtc actaaatata
     2641 tatatgttca aagtagggaa caaagctgtc tatccggctc acggggttgt gcaggtgaag
     2701 gcggtggaag caaaggaaat ctgcggaact aaaagaaatt tttacattct gcaggtcgtg
     2761 gacagcgatg ttaccgttat ggtgccgacc gataacgccg agactgtggg cctcaggccc
     2821 gtgataacta agagacagat aaccaagata tacgatatcc tcaagacaaa gatcaggaat
     2881 tcaaaccacc agaacggaag ccaaagctgg aacaaaagat accgcgaata ttccgacaag
     2941 ctcaagagcg gagacatatt cgaggtcgca gtcgtgctca gggacatcaa ccacctccag
     3001 aaggacaagg acctttcgtt cggcgagaag aggataatgg attcggcccg cacgctcctc
     3061 atcaaggaaa tatccatagc gaagaacctc ggagaggatt cggtaacgaa ggaaatagaa
     3121 aagctgctcg gctaaaccgg gtgaatccgc ctcctccctc ggttatactt tcccaatcaa
     3181 accggttaga agttaaatca tggcaaacgg catactcgac atagagaccg cttcgcgtct
     3241 caggcgcttt tatttcagga gcccgtcccg cgtcaggggc aggaaagcag gactccacaa
     3301 gagcctctac aaaggtatca gccccgattt cctcgaatac aaggaataca acagggggga
     3361 cgagctgaga caggtggact ggaggctcta cggccgccac gacaggcttt acgtaaaaaa
     3421 attcgaggac gaagtcaacc tcgcctggtg catcctggtc gacagctcgg cctctatggg
     3481 ttacggcgag ggcgtctccc ggaagctccg ttactcggcg ggcctggcgg cgactctcgc
     3541 ttatctactg ctgagacagg gggattccgt cggcggaggc gcgttctctg cggacgggct
     3601 gtcagttata ccgcccaggg ccgggaattc gtacatagcc cccatactgg aaaaactcga
     3661 gacgctcgcg cccgcgggga tgaccgcgct ctcggagccg ctcttgaaag cgctcgaagt
     3721 gttcagacag gacgcttcgt tcgtcattat atccgacctc ctcacggacg agagagagat
     3781 cgagaagtcg cttcagctgc tcagggccgc gaaaaaggac gcggtcatat tccacgtgct
     3841 gcacgaggac gaagtcgagt tctccttcag cggctccctc gagttcctgg atatggagtc
     3901 cgacgaaaag gtcatagtgg atacggatgc cgtgagggac ggttataaaa gaaggatcag
     3961 ggagttcacg gagaggctca aggtcctctg ccacgagtac gaatcgaggt acgtgttctc
     4021 gccctcctca aggccgtacg aggaatccct tatcgagatc gccgataaat agagagagtg
     4081 gcattgaatt tttccttcct taacccgctg ttcctgatag ggctcgccgc agtcgccctg
     4141 cccatcatcg cgcacctcat atcgaggaag agcggcgtca cgaaaaaatt cccggccgta
     4201 cggttcctca tcgcctcgca gggagacgcg tcggcgaggt cgaggctgaa ggacctgctt
     4261 ctgctcctgc tgagggcttg ggtgatagtg ctgctcgcgc tcgtcttctc gaagcccgcg
     4321 ctcttctcgc tcgctcctgc aggagcgagc gacccgaaat cgctcgccat catcgtcgac
     4381 aactccttca gcatggtata cggggataac ttcgggctcg cgagggagaa ggccgctgag
     4441 ctgatagaca cactccccga cgggagcttc gccattgtcg ccccgctcgt cacgcctgaa
     4501 ggggtcaggc tctcgccgtc gggcgacaga gaagcgctcc gggacgcgct cggagagata
     4561 aagatatcgg gctcgttcac ccggaacgag aagaggctcg aagaggcgct cggcgccatc
     4621 gacggaacgc cggggggcgc gaaggaagtc gtattcataa cggacttgca gaagaacgga
     4681 tggacggggg aagacgccgg gaagggctgg ctcaggttga tagacgtctc accggggcct
     4741 tcgcccgcga accgggcagt gacctccata gacacggcat acggcaagga ttcggtaacg
     4801 ctcaggctct acgtttccaa tttctcggac aagcccgagg attctcttct ggccacagca
     4861 gcgctgggcg gcgaggagtt gagcgcctac ctcgatatcc cgcccggagg gaccgccggg
     4921 aaggagctca ccattcccat cggacccgtc ccaggggata aatccccgga cgccgtccag
     4981 gaaatatcgg gcgaaggctc cgtctcgata ccgcgggata gtctccatat cgacgatacg
     5041 agatatttcg tcctctccag gagggacgtt ttaggcgtgc tcattgtgga cggggacccg
     5101 agggaggacg cgagactgtc cgagacctac tacctggcga gggcagccga gactatatcc
     5161 gagatcacgg gcgcacactt aacggtaaag gacaacgaca gcttcctcga cgagaagctc
     5221 tcggggtacg acatggtgtt cctcgcgaac gtcggagaca tcacggaggc gagcgcgaaa
     5281 gagctttcgg atttcgccgc gcggggcggc acggtcgtga tattcctcgg cgagcgcgtg
     5341 agagcgtctt catacaacgc gcttttaaaa gacacgctcc ccgcggagct gataaccgaa
     5401 gaggacaggg agacgaggat aatcccgggg cccggaaaca catttccgaa cgacgtcgcc
     5461 gagaggctca gccaggtcgg tgtcaggaag tacatcaagg cccttccttc ccaggacgct
     5521 caggtcctgc tgagtcttga aaacggagac ccgttcatga tagaaagacc tctcgggaat
     5581 gggagggtgt tcctcgtcac gtccacggcc gacacgagct ggaacaattt ttcgataacc
     5641 ccggtgttcc ttccggtcgt gaagagtctc ctcgacatac cggggtcggt gaacgagggc
     5701 aggagaaact atcaggtagg cgacacggtc ccgctcgaca taccggcagg cgcggtcagt
     5761 gtggaagtga taaacccctc gggaaagaaa aacgctctgg atacggccac agccgctttc
     5821 gacgggacgt acgagcccgg aatatacacg gtaaaaacat caggcaagga tttatacaaa
     5881 ttcgcagtga acgtggaccc aagcgaatcg aacctcgaga aga
//
LOCUS       DASSJH010000255         5495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1248344, whole genome shotgun sequence.
ACCESSION   DASSJH010000255 DASSJH010000000
VERSION     DASSJH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248344"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFJ67_05490"
     CDS             complement(<1..655)
                     /locus_tag="VFJ67_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET7289874.1"
                     /translation="MNDQWLFKSETYDNCNCAVNCGCQFNLPSTHGYCQSAFVGNIVE
                     GHFNDTPLAGLNWAALYKWPGEIKDGNGKRQIVIDERTDEAQRAALETIISGGACKPL
                     SNLFSVFASTCSEFCETLFLPIGLEANPELRTARVDIPGIMRSSGRPIINEFNGEPFH
                     VALARPSGSFEFTYAEIGLGTTSVTGDMEMAYEDSWAHYCVHHFNQDGLVRQRSRLTA
                     "
     gene            complement(1022..1447)
                     /locus_tag="VFJ67_05495"
     CDS             complement(1022..1447)
                     /locus_tag="VFJ67_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HET7289875.1"
                     /translation="MYLKIVAAVLIVAFIGIQFIFIERTNPPITGEIDAPAPVKEILM
                     RSCYDCHSNETVWPWYSYIAPASWLLEDDVNEGRAELNFSEWNGYDEKKKQKKIKETW
                     EEVNEGEMPQWYYVIMHPEAKLSDADKQVIRDWTAGWGK"
     gene            complement(1554..2396)
                     /locus_tag="VFJ67_05500"
     CDS             complement(1554..2396)
                     /locus_tag="VFJ67_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289876.1"
                     /translation="MEVIIAWLTIASLILIVIGLIDPAIALPWSREKTRKRAAMVYGY
                     AFFAMVALSIVFFPRTNLESRLYALFFLCIAALAAGFAYPKVVLPWARDASRKAVLLF
                     YLPPAVLLICAMVYANASRQVDPRYYIPPDELAGADQVMTLRYRAAAELKGQNNIGLD
                     RVRKIDVTGAKGGGYDMLVEFNIDDVMIPSFFKLKAESDMTNVYKAVYTSGYDVKNVT
                     VTAYFPVGYREKEGETPPVPVLTTSLAASAAAGVDWSLSPYDLETIVLPKAWKVEFKR
                     EGFE"
     gene            complement(2547..2972)
                     /locus_tag="VFJ67_05505"
     CDS             complement(2547..2972)
                     /locus_tag="VFJ67_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289877.1"
                     /translation="MRKILLSSAALAVLLTVVSLGNIGGCGGSNICDFGISSIYNGAS
                     FDDQDSEWDCRTGGVDEYTIAFFADGTGNRSDLGDFTWVQTECKQLEFTTDEGDFATL
                     RGLSVTGGIINGEKFGQLKFNQVSEDLGNFGYTCELVVF"
     gene            complement(3116..3913)
                     /locus_tag="VFJ67_05510"
     CDS             complement(3116..3913)
                     /locus_tag="VFJ67_05510"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7289878.1"
                     /translation="MRKPAVAENLSFWWGLMERFMSFDGVELAYMDSCGKGSPLFLLH
                     GFTTSCMIDWLETGVYNALAGAGRRVVMLDMRGHGESAKPHSAVSYCNRAMARDVHAL
                     AGSLGISEFGLMGYSMGAKVAVEAALLSEGIRRLVLVGFAVYESGWQYNEAERLARVK
                     NMLSRKSGKGDAYRLMAERYHGDRRAFAARLEGALLPEFTARDLLRIDVPVLVANGCD
                     DEVGAQRAASYFIRGYGKTFGGDSRSILENQKFPGLVLEFLDSDEYV"
     gene            4525..>5495
                     /locus_tag="VFJ67_05515"
     CDS             4525..>5495
                     /locus_tag="VFJ67_05515"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET7289879.1"
                     /translation="MKKTALLIAFSLILARAALAAPFAYITNEMDDTVSVIDTATNEV
                     VALVPTGDEPRGVAVSSDGKYVYVTNFLGNSVSVIDTSLNRAISTIRVGERPQCVAVS
                     PDGSRAYVTNFKSNTVSVIDTAAGKVIASVPVGTRPDGVAITPDGRFVYTANFEADTV
                     SVIDTSDNKVKATVASTHRPSGIVVSPDGAFVYVTNIASGDVTVIKTVGNRLLRTVKA
                     GMLPAGVVVSKDGRTVYTANSGSSDVTIIDTEEYEVTATIPAGPLASGADLTPDGKFL
                     YVANSGDKTVTVIDTAEKKVAATVKVGESPLAFGKFITQGSPPAPSPG"
BASE COUNT         1213 a         1630 c         1468 g         1184 t
ORIGIN      
        1 atgctgtgag cctcgatctt tggcggacca acccatcctg attgaagtga tgaacacaat
       61 aatgcgccca tgaatcttcg tacgccatct ccatatcgcc cgtcaccgag gtcgtgccta
      121 gtccgatttc tgcataggta aactcgaaac tgccgctagg acgtgccagc gcaacgtgga
      181 acggctcacc attaaactcg ttgattatcg gtctgccgct gctcctcatg atgcccggta
      241 tatcaaccct tgcggttctg agttccgggt tcgcttcgag accaatcggc aggaacaatg
      301 tctcgcaaaa ttccgagcat gtggacgcga atacggagaa caaattgctc aggggtttgc
      361 atgccccgcc tgagataatt gtttcgagtg cggcccgttg agcttcatcc gtacgttcgt
      421 cgataacgat ctggcgtttg ccatttccat ccttgatctc gccgggccac ttgtacagcg
      481 ccgcccaatt cagccccgcg agcggcgtat cgttgaagtg gccttcaacg atgttaccga
      541 cgaaggccga ctggcaatag ccgtgggtgc taggcaagtt aaactggcaa ccgcaattca
      601 cagcgcaatt gcagttatcg taggtttcgc tcttgaacag ccattgatca ttcattcgcc
      661 ggccctcccc tcaatgcgct tcaaataagg tttgttatgg catcactgct gtctaggcgt
      721 tcagtttgaa tggtcgctta ggttatatgt ggcccgcctt tcccgccgat tgtcgacccc
      781 ccagccgtca ctcatctctg ccacataact cataggagac ttcgttggat caataagatt
      841 ataaaatttc cgggccgtat agtcaataga atgtcaagcc cctgggaaac caatccgcct
      901 tttcagttag attcccttca gaaatcccgc ataatggtat tcgcaaaaat agattgagag
      961 catttgcatg tttcaactta aatcgagctc gtcaaacggg gagcggtaca aacaaacttc
     1021 cctacttccc ccaccccgcg gtccagtcgc ggatgacctg cttgtcggcg tctgacagct
     1081 tcgcctccgg gtgcatgatg acgtagtacc actggggcat ctccccctcg ttcacctctt
     1141 cccacgtctc cttaatcttc ttctgcttct ttttctcgtc gtacccgttc cactcggaga
     1201 aattcagctc cgccctcccc tcgttaacgt cgtcttcgag tagccaggac gcgggcgcga
     1261 tgtaggagta ccagggccac acggtctcgt tcgaatgaca gtcgtagcac gagcgcatga
     1321 gtatctcttt caccggggcg ggcgcgtcta tctcgcccgt gatcggcggg ttcgtcctct
     1381 caatgaatat gaactgtatc cctatgaacg caactatcag cacagcagcg acaatcttta
     1441 aatacaccac cgcttccgga cctccggttt tttagtaaag tcgggactgc ttctcttagt
     1501 cgcagatatt ataatggaaa ctcgggccta atccagaccg cgccggttaa aaatcactcg
     1561 aaaccttctc gcttgaactc gaccttccag gcctttggca gcactatggt ctcgagatcg
     1621 tacgggctca ggctccaatc gacccccgcc gcggctgagg cggctaacga cgtggtcagc
     1681 acgggcacgg ggggcgtctc cccttccttc tccctgtatc cgaccgggaa ataggcggtc
     1741 acggtcacgt tcttcacgtc gtagcccgag gtatagacgg ctttgtaaac gttcgtcatg
     1801 tcggactccg ccttgagctt gaaaaatgaa ggtatcatga cgtcgtcgat gttgaactcg
     1861 acgagcatgt cgtatccccc gcccttggcc ccggtcacgt cgatcttcct cacgcggtcg
     1921 agcccgatgt tgttctgccc cttcagctcc gcggcggccc tgtacctgag cgtcatcacc
     1981 tggtcggccc cggcgagctc gtcgggaggg atgtaataac ggggatcgac ctgcctggat
     2041 gcgttcgcgt acaccatagc gcagatgagg agcacggcgg gagggagata gaaaagcagc
     2101 accgccttcc tcgacgcgtc cctcgcccac ggcagcacta ctttcgggta ggcgaagcct
     2161 gcggcgagag cagcgataca gaggaagaag agagcgtaga gcctgctctc gaggttggtg
     2221 cgcgggaaga acacgatgct gagcgcgacc atggcgaaaa aggcgtagcc gtacaccatc
     2281 gccgccctct tccgcgtctt ttccctcgac cagggaagcg cgatcgcggg atcgatcaga
     2341 cctatcacaa tcaatatgag tgatgcgatc gtaagccagg ctatgataac ttccattcga
     2401 taaaacgctc cggtttgctc gcaccccagt ccgcccgggc gtacccgcgc caacaaattt
     2461 accctcccgg cgtattaaca caaacgcgtc cgggcatacg gttagtcaca ataaggaaca
     2521 agcggcgctg atttagggag cgcggcctag aagaccacga gctcgcaggt gtatccgaag
     2581 ttccccaggt cttccgagac ctgattgaat ttcagctgcc cgaacttctc tccgtttatg
     2641 atgccgcccg tgacagacag gcccctgagt gtcgcgaaat caccttcgtc cgtcgtgaat
     2701 tcgagctgct tgcattcggt ctgcacccag gtgaagtccc cgaggtccga cctgttgccg
     2761 gtgccgtccg cgaaaaacgc gatcgtgtat tcgtcgaccc cgcccgtcct gcaatcccat
     2821 tcggaatcct ggtcgtcgaa actcgctccg ttataaatgc tggatatgcc gaagtcgcag
     2881 atgttgctcc ctccgcaccc tccgatgttc ccgagcgaca ccacggtcaa aaggaccgcg
     2941 agcgcggcgg aagacaatag tattttcctc atggcctgca tcctccttga gatgttttag
     3001 gcgaaaattt attactcccc cggaaggaaa gttacaccaa ttatatatca taatgtgcga
     3061 gagaaatacg gccggacggc cgggaccgtc ataaagccgg gccgggcgcg cgccctcaga
     3121 catattcgtc cgaatcgagg aactcgagca cgaggcccgg aaacttctgg ttttccagta
     3181 tggagcggct gtcgccgccg aacgtcttgc catacccgcg gatgaagtac gaggcggccc
     3241 tttgagcgcc cacctcgtcg tcgcacccgt tcgcgacgag cacggggacg tctatcctca
     3301 ggaggtcccg cgcggtgaat tcgggaagga gagccccttc gagcctcgcg gcgaacgccc
     3361 tcctgtcacc gtggtaacgc tccgccatga gcctgtacgc atcccccttg ccggacttcc
     3421 tgctgagcat gttcttcacg cgggcgagcc tctcggcctc gttgtactgc cagccgctct
     3481 cgtaaactgc gaaccccacg agaactagcc tccttatgcc ctcagacagg agcgccgcct
     3541 cgaccgcaac cttggctccc atcgaatatc ccatgagccc gaattcggat atccccaggc
     3601 tcccggcgag ggcgtgcacg tccctggcca tggccctgtt gcagtaggag acggcggagt
     3661 ggggcttggc cgactccccg tggccgcgca tatcgagcat gaccaccctc cgccccgcgc
     3721 ccgcgagcgc gttatagacg cccgtctcca accagtctat catgcaggag gtggtgaagc
     3781 cgtggaggag gaagagaggg gatcccttcc cgcaggagtc catatacgca agctcgaccc
     3841 cgtcgaagct catgaacctt tccataagtc cccaccagaa cgacagattt tcagcgacgg
     3901 caggttttct caagaaagag ttcgtgaccg ggcccgatcg tacggaggga gaaacggtgg
     3961 atgctgcaaa atcagagggg gaggagaatt ccgaagttct atctacgtat ccgcaggtat
     4021 taccgaccgc cagtttctat tatacaattt cgcgcgacag atttgaagag acgattccga
     4081 acgagtgagg aatcggacgc ccttgaaaaa tgacggaatg caactgttct tcgctaaaca
     4141 ttactgtaac cgtagcgaga aagccatctt tttatttgtc cttccgacat tacagaattt
     4201 gcgttaagaa aatagaatat taaggcgtaa aaatgtttta gggatggggt ggaattaaaa
     4261 cattttcgcc ttaatgtgag atttttagca aattatgttc tgtcggttga tttttgtttc
     4321 ttttcatcaa ggaaaagaag gataaaaaga ataatctttc ttctctccct gtctttttca
     4381 caattcatca tcacataaac ccttgtccta ccagtttcag gtcggattta acaacggaaa
     4441 atcgcagtat tcttaacccc cgcaatgttg tattatttgc tgaacggggg cgggctcagc
     4501 ctcccccggg ccgaggtgtg agagatgaag aaaacagcgc tcctgatcgc cttttccctg
     4561 atattagccc gggccgccct cgccgcgccc ttcgcctaca tcacgaacga aatggacgac
     4621 accgtctcgg tcatagacac ggcgaccaac gaggtagtcg ccctcgtccc gacaggggac
     4681 gagccgaggg gggtcgcggt cagctcagac ggcaagtacg tgtacgtcac caacttcctc
     4741 ggcaacagcg tctccgtcat agacacgtcg ctcaaccggg ccatctcgac catccgggtc
     4801 ggggagaggc cccagtgcgt cgccgtgagc ccggacggca gccgcgccta cgtgacaaac
     4861 ttcaagagca acacggtctc ggtcatcgac acggccgcgg gcaaggtcat agcgtcagta
     4921 cccgttggga caaggcctga cggcgtcgct ataacgcccg acggcaggtt cgtttacacg
     4981 gcgaatttcg aagccgacac ggtttcagtc atagacacgt cggataataa ggtgaaggct
     5041 acggtcgcga gcacccacag gccgtcgggc atcgtagtga gcccggacgg cgcattcgtt
     5101 tacgtaacca acatcgcgag cggggacgta acggtgataa agacggttgg gaacaggctg
     5161 cttcggacgg tgaaggccgg gatgctgccc gcaggggtgg tagtaagcaa ggacggcagg
     5221 acggtttaca cggcaaattc cggcagcagc gacgtgacga tcatcgatac ggaggaatac
     5281 gaggttacgg caaccatacc ggcggggccg ctcgcctcgg gcgcagacct cacgcccgac
     5341 gggaaattcc tctacgtcgc caactcgggg gacaagaccg ttacggtcat agacacggct
     5401 gagaaaaagg tcgctgccac ggtgaaggtc ggcgagagcc cgctcgcctt cggcaagttc
     5461 atcacgcagg gaagcccgcc cgccccgtca cccgg
//
LOCUS       DASSJH010000256         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1032481, whole genome shotgun sequence.
ACCESSION   DASSJH010000256 DASSJH010000000
VERSION     DASSJH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032481"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFJ67_05520"
     CDS             complement(<1..311)
                     /locus_tag="VFJ67_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289880.1"
                     /translation="MKVSDLRDSPGIWLFLIMLLILALRLPTTHVSVIDWDESVYFTI
                     AQDITNGGVPYKTAWDTKGPLLFFIFVPVILLFDENIAALRVFTTAYLLLSMFFLYLL
                     A"
     gene            729..>2556
                     /locus_tag="VFJ67_05525"
     CDS             729..>2556
                     /locus_tag="VFJ67_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET7289881.1"
                     /translation="MTKSQKYIPIFIFTILLIAIFLILGPSRFNKPVKGSSETLSVRT
                     ENDFVVDPGLMALPAEDIVAVLLESTDCKVLDKDTGGKGYDIIPYRYASSMEQTFCWE
                     DDNRNAGHYMVLVNDEGEEVLQVNANGECVTKEIGAGDYELRIYHDAETEKNVAVFIV
                     PENGKSILSTASGETLDSVSTFLETDKCEGCDLSGANLYNADLRGVDLEGAKLNDAIL
                     ANANLSGANLKGTELRNADLTGADMTGANLAGANLAGAILINSDLTDANLLRANLENA
                     DLTGANITEAQLAGAVTTGVLRNSVKSDETVGTKVHDDVVATITCTTGAIAAGAGEDL
                     DITGPCTVDGTVPNGVYHYRNVNIYNGGSLTFNDAVIHFWAYNILVEYNGSLIAGSEM
                     TPIGAAGGKLTIHLYGEDQGVGGKGVLCKTGDMCGVDTNIWNNDHTSKQTLPGPVEDY
                     FYHYHPLPADTGDAHGYFGYKTLGVSYNGTLRLFGKKGALYPDTNPTPSDSGMSWARL
                     SGDVNVGATQLTVDRDVDWGVGDRIVVTTTDYLPAHSEVLEIIAPAVSATTFNFKVIN
                     PFTGAGISGGLQYKHSGTPYPLTGVPNINIKVKNQPAAETRAA"
BASE COUNT          692 a          605 c          732 g          527 t
ORIGIN      
        1 ctggccaata aatagagaaa gaacatcgaa agaaggaggt aggcggtggt aaaaacgcgg
       61 agagcagcga tgttttcatc aaaaagaaga attacaggca cgaatataaa gaataaaagc
      121 gggccttttg tgtcccacgc ggtcttgtaa ggcactcctc cgttggttat gtcctgagca
      181 atcgtgaaat agacagactc atcccaatct attacggaga cgtgcgtcgt cggcagcctc
      241 agggcaagaa tcaacagcat tattaagaat aaccatatgc ccgggctatc cctcagatcg
      301 ctaactttca cttactcctc cttttctaag tggagatata ttagtacatt ggaatcgatt
      361 tgtgaaccgg gacaataaat cctgccgaaa aactccggga atccacccat ggcgcggatt
      421 attgaaataa cccggaaaat tataatgctt tctcgatgga atcgggtgta ttcaatcggt
      481 aggcgtgttc tgtgacggct gtatccgcag taggttatgg ccgcatatac catccgtccg
      541 cgttatgaaa ttcgtaaata attataggta gaatttgcgt tccggaagaa acggtaacca
      601 tatcgtcgtt cacgatatga aagttctgcc gctatttgca gtgtttgcaa aatcaattga
      661 ttagcattat gtttacaaaa tatagtccat tcatagagtt gtaccgatta agaggaggat
      721 gatcaaccgt gacaaagtcc caaaagtaca tccccatatt tattttcacg attctattaa
      781 tagcgatttt tttaatcctg ggtccgagca ggtttaacaa gcctgtgaag gggagctcgg
      841 agacactgag cgtacggacg gagaacgact tcgtagtaga ccccgggctc atggccctgc
      901 cggccgaaga catagtggcc gtgcttctgg agtcaaccga ctgcaaagtt ctcgataagg
      961 acacaggggg gaaggggtac gacataatcc cttaccggta cgcgtcgtca atggagcaga
     1021 cgttctgctg ggaagacgat aacaggaacg cggggcatta catggtgctt gtgaatgacg
     1081 aaggggagga ggtgctccaa gtcaatgcga acggcgaatg cgtgaccaag gagatagggg
     1141 cgggggatta cgagctgagg atttatcacg acgcggagac agagaagaac gtggccgtgt
     1201 tcatagtgcc agagaacggg aagtcgattt tgagcacggc ttccggggag acgctcgaca
     1261 gcgtgtcgac gttcctcgag acggacaagt gcgaggggtg cgacctatcg ggagcaaacc
     1321 tgtacaatgc ggacttgagg ggagttgacc tcgaaggggc caagctcaac gacgcgatac
     1381 tggctaacgc aaacctgagc ggggctaacc tgaaagggac ggagctcagg aacgcggacc
     1441 tcacgggagc ggacatgacg ggggcgaacc tcgcaggggc aaacctcgcg ggagcgatac
     1501 tgatcaattc cgatcttacg gacgcgaatc ttctccgggc gaatctcgag aacgccgatc
     1561 taaccggggc taatatcacg gaggcgcagt tggcgggggc ggttaccaca ggagtcctgc
     1621 gaaacagcgt taaatcagac gaaacggtcg ggacaaaagt acacgatgat gtggtcgcaa
     1681 caataacatg tacgacgggc gcaatcgcgg cgggggccgg tgaggacctc gatataacgg
     1741 gtccgtgtac cgttgacggt acggttccga acggggttta ccattaccga aacgttaaca
     1801 tatataacgg cggatccctc acctttaacg acgccgttat tcatttctgg gcatacaaca
     1861 tactcgtaga atataacggg agcctaattg cgggttccga aatgactccc atcggcgcag
     1921 ccggcgggaa gcttacgata catctttatg gtgaggacca gggcgtcgga gggaagggcg
     1981 ttctctgcaa aacgggcgac atgtgcggcg tggataccaa catatggaac aacgaccata
     2041 cgagcaagca gacgctgccg ggtccggtag aggattattt ctatcactac catccgctgc
     2101 ccgccgatac gggcgatgca cacggctact tcgggtataa aaccctcggg gtttcataca
     2161 acgggactct cagattattc ggaaagaagg gtgcgctcta tcccgacacc aacccgacac
     2221 cctccgattc aggaatgagc tgggcgcgtc tgagcgggga tgtaaatgta ggagccacac
     2281 agctcacggt ggatcgagac gtcgactggg gagtcgggga caggatcgta gtcaccacga
     2341 ccgactacct tcccgcgcac tcggaggtcc tggaaataat agcgcccgcc gtcagcgcaa
     2401 cgacgtttaa tttcaaggtc ataaatccgt tcacgggcgc gggaatatcc ggaggcttgc
     2461 agtacaagca cagcggcact ccgtatcctt tgacgggtgt tccgaatatc aatataaagg
     2521 tcaagaacca gcctgccgcg gaaacgaggg cggcgg
//
LOCUS       DASSJH010000257         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_922832, whole genome shotgun sequence.
ACCESSION   DASSJH010000257 DASSJH010000000
VERSION     DASSJH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922832"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFJ67_05530"
     CDS             <1..788
                     /locus_tag="VFJ67_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HET7289882.1"
                     /translation="NRTVGTILGSEITRRYGTDGLPPDTVRLHFNGSAGQSFGAFIPN
                     SMTLILEGDSNDYIGKGLSGGKVIVYPPKGSTFKPEENIIIGNVAFYGATGGEAYIRG
                     MAGERFCVRNSGVHAVVEGVGDHACEYMTGGRVLVLGSTGRNFAAGMSGGVAYVLDEI
                     GDFSVRCNKEMVSLDKLGDEDIEEIKGMIERHVEYTHSERGRDILSRWTGLSEKFVKV
                     MPKDYKRMLEAIRKVQKTGLSGDEALMAAFEENKRDLARVSGN"
     gene            862..>2135
                     /locus_tag="VFJ67_05535"
     CDS             862..>2135
                     /locus_tag="VFJ67_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET7289883.1"
                     /translation="MGDPTGFMEHDRELEPDRSPKERIKDWKEFHKHLPEEKLRVQGS
                     RCMDCGIPFCQSGTIINGLTAGCPVNNLIPEWNDLIYKGLWKEALERLHKTNNFPEFT
                     GRICPAPCEGSCVLGINEPPVTIKTIECTIIDKGFEEGWVVPEIPETRTGKKVAVIGS
                     GPAGLACAAQLNKAGHWVTVFERDDRIGGLLMYGIPNPHLEKSVVERRVKLLAEEGVK
                     FVTNTEVGKDYPVGKLLKEFDAVVICTGATRPRDLPVEGRELKGIHFAMEFLKANTKS
                     LLDSGLKDKNFISAKDKDVIVLGGGDTGTDCVATSMRHGCRSLLQFEILPKPPFERAP
                     DNPWPQWPKVYTISYGQEEAMAVFGADPRKYQVLTTRFAGDGEGNVKELHTIQIEWHM
                     SENGRTSFREIPGTEKVWPAQLVFLALGFLGP"
BASE COUNT          548 a          531 c          665 g          391 t
ORIGIN      
        1 tcaacaggac ggtcgggact atactcggga gcgagataac gaggcgttac ggaacggacg
       61 gtctcccgcc cgatacggta aggctccact tcaacggctc tgcgggacag agcttcgggg
      121 cttttatacc taactccatg acgctgatac tcgaaggcga ttcgaacgat tacatcggca
      181 agggcctctc ggggggcaag gtcatagtct atccgcctaa gggctcgacg ttcaaacccg
      241 aagaaaacat aataataggc aacgtagcct tctacggcgc aaccggaggg gaggcgtata
      301 taaggggcat ggccggagag cgcttctgcg tcaggaacag cggggtccac gcagtcgtcg
      361 agggcgtcgg cgatcacgcc tgcgaataca tgacaggcgg gcgcgttctc gtgctcggat
      421 cgaccggcag gaactttgcg gcagggatgt ccggaggggt ggcgtacgtc ctagatgaga
      481 tcggagactt ctccgtcagg tgcaacaagg agatggtaag tctcgataag ctcggggacg
      541 aagatatcga agagattaaa ggcatgatag agcgtcacgt ggagtacacc cacagcgaaa
      601 ggggaagaga cattctctcc cgctggaccg ggttatctga aaaattcgta aaagtcatgc
      661 ccaaggatta caagaggatg cttgaagcga taaggaaagt ccaaaaaacc ggcctcagcg
      721 gggacgaggc cctcatggcc gcgtttgaag aaaataaacg cgacctggcc cgtgtaagcg
      781 gcaattaatt ctctccgatt ttcgtacaag taagccaaaa tccggtataa tcctatcgat
      841 tttgaactgg aggaagtctt aatgggtgac cctacaggtt ttatggagca tgatagagag
      901 ctcgagcccg accgctcccc gaaagagcgg ataaaggact ggaaagagtt ccataaacat
      961 cttcccgagg aaaagctcag ggtacagggg tcgaggtgta tggactgcgg gataccgttc
     1021 tgccagtcgg ggaccatcat aaacggactg acggcgggct gccccgtaaa caacctcatt
     1081 cccgagtgga acgacctcat atacaaaggg ttatggaaag aggcgttgga acgtctccat
     1141 aagacgaata actttcccga attcacgggg aggatatgtc ctgccccctg cgagggttcg
     1201 tgcgtgctcg gcatcaatga gcctccggtt accataaaga cgattgaatg cacgataata
     1261 gacaaggggt tcgaagaagg gtgggtcgtt ccggagatac ccgaaacgag gactggcaag
     1321 aaggtcgctg tcatcggctc cggcccggct gggcttgcct gcgcggcgca gctcaacaag
     1381 gcggggcact gggttaccgt attcgagagg gacgacagga taggggggct cctcatgtac
     1441 ggtataccga atcctcattt ggaaaagagc gtagtcgaga gaagggtgaa gctgctcgct
     1501 gaagagggag tgaagttcgt aacgaacacc gaggtgggca aagactaccc ggtgggcaag
     1561 ctgcttaagg aattcgatgc ggtcgttata tgcaccgggg ctacgaggcc ccgcgacctg
     1621 cctgtcgagg gcagggagct caaaggcatc catttcgcca tggagttcct gaaggccaac
     1681 accaagagcc tcctcgacag cggtctcaag gacaaaaatt tcatatcggc caaggacaaa
     1741 gacgttatag tcctcggcgg aggcgatacg gggaccgact gcgtcgccac atcgatgagg
     1801 cacgggtgca ggagcctgct tcagttcgag atattgccca agccgccttt cgagcgggcg
     1861 cccgacaacc cctggccgca gtggcccaag gtttatacga taagctacgg acaggaagag
     1921 gcgatggccg tattcggggc cgacccgagg aaataccagg tcctgacgac caggttcgcc
     1981 ggagacggtg aggggaacgt aaaggagctc cataccatac agatagaatg gcacatgagc
     2041 gagaacggac gcacttcgtt cagggagata ccgggcacgg agaaggtatg gcctgcccag
     2101 ctcgttttcc ttgcgctcgg attcctcggg cctga
//
LOCUS       DASSJH010000258         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_496354, whole genome shotgun sequence.
ACCESSION   DASSJH010000258 DASSJH010000000
VERSION     DASSJH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496354"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VFJ67_05540"
     CDS             complement(<1..924)
                     /locus_tag="VFJ67_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HET7289884.1"
                     /translation="MITKKTVDRADFHLKIVQEISDLVNKSTGLNTILKKVVNKVASS
                     LHFDVVSVYLWDRDNNVLRLRSTKGLHVDPMKTPITLNAEEGLTGLVFETSRAITVMP
                     ASEHPRYRYFPEIGEEEYESYIGVPIILQNRCIGVLVGQTKDKRLINPAEETLFQIIA
                     SRLAGLLEVADRLERLKTPSIVKHETRTYQGKGVSTGVAIGSIFIFRGLFQQISADKL
                     RPSSPKVEKGRLKKALREVEEDLRDLIKTLDSDALLSKAEIDIFRAHLMILEGATLRN
                     AISQKLKEKNIPAELAVIEGIEPIAQQFEGLS"
     gene            1268..2686
                     /locus_tag="VFJ67_05545"
     CDS             1268..2686
                     /locus_tag="VFJ67_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7289885.1"
                     /translation="MDKSSDGKKVIKAAIVGSGPAGFYTAEHMLKRKDADFRVDMFDR
                     LPTPHGLVRSGVAPDHQKIKSVTKVYDKIASDPRFRFFGLVEFGKHVTLEDLKKHYHV
                     VVFSTGAQTDRRLGIPGEELEGSHTATEFVAWYNGHPDYRHLRFDLSGEKAAVIGVGN
                     VAVDVARILCRSTEELKETDIADYALEALAGSRIKDVYLLGRRGPLQAAFTNPEIREL
                     GKLSGAHPVTLPHEVGHDEFTEEALGEGKDQTAVNKLEILKSFSDPAHHTKDKRLHIR
                     FLVSPVEIIGDERGHVRALKLVRNELYRAEDGSVRSRPTDKYEVLGTDIVFRSIGYQG
                     VPLPGVPFHEKWGVIGNEKGRVTDPGTGGHMIGLYVCGWIKRGPTGVIGTNKQDAGET
                     VECIAEDVANGAINVPKNPGADCVLDLVSQAQPDYLTYDDWIRIDNLEIERGKALGRP
                     RLKFTSVEEILEALKSAKKAAE"
BASE COUNT          642 a          768 c          846 g          604 t
ORIGIN      
        1 actcaggcct tcgaactgct gggcaatggg ttctatgccc tctattaccg cgagctcggc
       61 cggaatattc ttttccttga gcttctggga tatggcgttc ctgagggtcg cgccctcgag
      121 tatcatgagg tgcgccctga aaatgtctat ttccgccttc gacaggagcg cgtcagaatc
      181 gagagtcttg atgaggtcgc ggaggtcttc ctccacctcc ctgagcgcct ttttaagcct
      241 gcccttctct accttggggc tcgaaggcct cagcttgtcg gcgcttatct gctggaagag
      301 tcccctgaat atgaatatgc tcccgatagc gacgcccgtg gagacgccct tcccctggta
      361 ggtcctcgtc tcgtgcttga cgatcgaagg ggtcttcagc ctctccagcc tgtcggccac
      421 ttcgagcagg cccgcgagcc tcgaagcgat gatctgaaat agggtctcct cggcggggtt
      481 tatgagcctc ttgtccttcg tctggccgac gaggacgcct atgcacctgt tctggagtat
      541 tatgggtacg ccgatgtagc tctcgtattc ctcctccccg atctcgggaa agtacctgta
      601 cctcgggtgc tcggaagccg gcatgaccgt gatggccctc gacgtctcga aaactagccc
      661 cgtgagacct tcctcagcgt taagcgttat aggggtcttc atcgggtcga cgtgtagccc
      721 cttcgtcgag cggagcctga gcacgttgtt atccctgtcc cataggtaca cggagaccac
      781 gtcgaagtgg agcgagctcg ctaccttgtt gaccaccttc ttcaggatgg tgttgaggcc
      841 cgtggattta ttgaccaggt cgcttatttc ctggacgatc ttcagatgga agtcagctct
      901 gtctacagtt ttcttggtaa tcatcaaaga acccggaccc ggcttataga atactccgcg
      961 gaacgcattg tccgccgcgc ctaacctgtt ccgatatgtt tatcgtctgc acctgcaatc
     1021 caagcacgat accggatacg cccgggagcg gaaatcgtcc cctggacgtc ctgcatgcgg
     1081 tgataaatac acccggcggg ttttttgggt atatttatcg cctgaattat aatactcatt
     1141 aaaaacccgg atgcataatt cccgaataaa aatataattg cgataacgaa cgggtcaatg
     1201 attaaacctg tctgcggcgg aaaacctgac ctctgcagcg ggacaaagga agataaggaa
     1261 cctgataatg gataagagca gtgacggaaa gaaagtgata aaggcggcga tagtaggatc
     1321 ggggccggcc gggttctaca cggccgagca catgcttaag cggaaggatg cggatttccg
     1381 tgtggacatg ttcgacaggc tgcccacgcc gcacgggctc gtgaggtcgg gcgtcgcgcc
     1441 tgaccaccag aagataaagt ccgtgacgaa ggtctacgac aagatagcgt cagacccgag
     1501 gttcaggttc ttcgggctcg ttgagttcgg caagcacgta accctcgaag acctgaagaa
     1561 acactaccac gtggtcgtgt tctctaccgg cgcccagacc gacaggaggc tcgggatacc
     1621 gggcgaagag ctcgaagggt ctcatacggc gaccgagttc gtggcctggt acaacggtca
     1681 ccccgattac aggcacctgc gcttcgacct ctcgggggag aaagcggccg tcataggagt
     1741 gggaaacgtg gcagtggacg tcgcgaggat actatgccgc tctacagaag agcttaagga
     1801 aacggacata gccgactacg ccctcgaagc gctcgccggg agccgcataa aagacgtgta
     1861 tctcctcgga aggagggggc cgctccaggc ggcgttcact aaccccgaga taagggagct
     1921 cggcaagctc tccggggcgc accccgttac gcttccgcac gaggtcgggc acgacgagtt
     1981 tacggaggag gcgctcgggg agggcaagga ccagaccgcc gtcaacaagc tcgagatact
     2041 gaagtccttt tccgatccgg cgcaccatac gaaggataaa aggctccata taaggttcct
     2101 cgtttctccg gtcgagataa tcggggacga gcgcggacat gtgcgagcgt tgaaactcgt
     2161 cagaaacgag ctctacaggg ccgaggacgg atcagtgcgc tcccgcccca cggacaagta
     2221 cgaggtgctc gggacggaca tcgtgttcag gtctatcggg tatcagggcg tgccgcttcc
     2281 gggcgtgccg ttccacgaga agtggggtgt gatcgggaac gagaaagggc gcgtgacgga
     2341 tcccggcacg ggcggtcaca tgatcggcct ctacgtttgc ggatggataa agagggggcc
     2401 gacgggcgta ataggcacga acaagcagga cgctggggag actgtggagt gcatagccga
     2461 ggacgttgcg aacggggcga taaacgttcc caagaatccc ggcgcggact gcgttctcga
     2521 tctcgtgtca caggctcagc cggattatct tacatacgac gactggatca ggatagacaa
     2581 cctcgagata gagagaggta aggcgctcgg gaggccgagg ctcaaattca cgagcgtcga
     2641 agagatactg gaggccctga aatccgctaa aaaagctgca gaatagccgt gacgggctat
     2701 ctataatata taaaataccg tattagatat atcatttaac cgactataga cattgataat
     2761 gtctttctta aaggatcgac tttcctgatc ctgatcctga ctctgtcgcc ctcggcggga
     2821 attttgtcgg ttaccaggcc ggccggggcc tcgaagaggt
//
LOCUS       DASSJH010000259         3200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_713257, whole genome shotgun sequence.
ACCESSION   DASSJH010000259 DASSJH010000000
VERSION     DASSJH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713257"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFJ67_05550"
     CDS             complement(<1..812)
                     /locus_tag="VFJ67_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7289886.1"
                     /translation="MSKSRAKASGIREIQSDFLIIGSGLAGLYAALYASRFGSVTILT
                     KSTVEESNSYWAQGGIAAVVDPEDSTWFHIEDTMRAGRGLNDMEAVTVLVNEGKDRVS
                     DLIKLGMKFDTGEKGLELGLEGGHTRRRVLHAGGSSTGREMVKFLIAAVENNKSIMKF
                     ESTGVVGFFSDGVSCSGALAIGVETGPVAFISKSTILATGGASALYERTTNPEGATGE
                     GIALAYEAGAEISDMEFEQFHPTAFYNKDGASFLISEAVRGEGAYLLDSGGK"
     gene            complement(855..>3200)
                     /gene="glnD"
                     /locus_tag="VFJ67_05555"
     CDS             complement(855..>3200)
                     /gene="glnD"
                     /locus_tag="VFJ67_05555"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET7289887.1"
                     /translation="PRNKTLAKEATEKLLYLLWDLGLDVGHSVRTIDECIGLSQEEDT
                     TILTSLLDSRFVCGGEQLYMDLDKKIYCQLLPTISHKFIQSKIRENEQRINRFGRSVY
                     LLEPNVKEGEGGLRDIHYALWIAQAKFKVKNFSDLLPKGILMENEIRIFEKSLNFLLL
                     IRSELHYLAGRREDRLGFEFQEKIAKFLGFKDAELPAVERFMRIYYLRGNELREQSKR
                     LIEKCLLDHKSGIRTAKTVTLDNGFIIQGGMLSASNINIFRDDPVNLMRAFEYADKYQ
                     VKMSNYLHDLIRENVSVTTMDEGVRSNPELNASFLRLLKKGKNVADTLFEMNRLRFLG
                     HYIPEFGKIVCMVQHDAYHVYTVDVHSIFMVREIENLLSYKYEMEHPLLTKTAESLVS
                     RHVLYLACLFHDMGKGEGKDHAAKGAAMIPKIAGRLGLDATETKQLEFLVENHLLMSH
                     VSQRRDIHDYSLVIRFAKSVKNLETLSLLYLLTFADIKSVGPDVWTNWKGMLLKELFI
                     RTAKVLERGSFKGEDPLARQKRVIDEVLRILGSKVSRRNVRAILETMPESYFLGFSPR
                     KIAYHVGLIEKSKDAVGMDVLFYPHEEYNEFTFWGFDEPGIFSKLCGVVRASGLNVLG
                     ARITTRKDGRILDVFYVNKLGQSVKEGEEVWDKLRDNLNKVLTGKKDVEELVARKKQE
                     KPIYSKAIPQYPTRIMVDNESSDAATVIDVITYDRAGLLYDITKTIKDLGLSIEYAKI
                     STKVDQVVDAFYVVDLSHKKITDPDRIEEIKTAIQESITSG"
BASE COUNT          624 a          934 c          818 g          824 t
ORIGIN      
        1 ctctttcccc cgctgtcgag aaggtaagcg ccttcgcccc gcacggcttc cgaaataagg
       61 aagcttgctc cgtccttgtt ataaaaagcc gttgggtgga actgctcgaa ttccatatcc
      121 gaaatctccg ctccggcctc gtatgcgagc gctatgccct cgcccgtcgc gccttcggga
      181 ttggtagtcc tctcgtagag cgcagacgcc ccgcccgtgg cgagtatcgt ggacttcgat
      241 ataaaagcga ccggcccggt ctcaacgccg atggcgagcg ctccggagca cgaaaccccg
      301 tccgagaaga accccactac gcccgtgctc tcgaacttca ttatcgattt attgttttcg
      361 acggcggcta tgaggaactt taccatctcc ctccccgtcg agctcccgcc cgcgtggagc
      421 acgcgtctcc ttgtgtgccc gccctcgaga ccgagctcga gccccttctc cccggtgtcg
      481 aacttcatgc cgagctttat gaggtccgag acgcggtcct tcccttcgtt gacgaggacg
      541 gtgaccgcct ccatgtcgtt gagccccctg ccggcgcgca tcgtgtcctc tatgtggaac
      601 caggtggaat cctcgggatc gacgacggcg gcaatacccc cctgcgccca gtaggagtta
      661 ctctcctcga ccgttgattt ggtgagtatc gtgacgctcc cgaatcttga ggcgtagaga
      721 gccgcgtaga ggccggcgag gccgctcccg attatgagaa aatccgattg tatttccctg
      781 atgccggaag ctttcgcacg cgatttactc attttcgaca tttatgtccg cagaattgat
      841 tttcgaaaag gaccttaccc ggaagttatc gattcctgta tggcggtttt tatttcttct
      901 atcctgtccg gatccgtgat ctttttatgg ctcaggtcca cgacgtaaaa agcgtccacc
      961 acctgatcga ccttggtcga gatcttcgca tattcgatcg aaaggccgag gtccttgatc
     1021 gtcttcgtaa tgtcgtagag gagccccgcc ctgtcgtaag tgatcacgtc tatcacggtc
     1081 gcggcgtcag aggattcgtt gtcgaccatg atcctggtcg ggtattgcgg tatcgccttg
     1141 ctgtatatgg gcttctcctg tttcttcctc gcgaccagtt cctcgacgtc cttcttgccc
     1201 gtaagtacct tgttcagatt atccctgagc ttgtcccaaa cctcttcccc ttccttcacc
     1261 gactgaccga gcttgttcac gtagaatacg tcgaggatcc ttccgtcttt cctcgtcgta
     1321 atacgcgcac cgagcacgtt caggccggac gccctgacga caccgcagag cttggagaat
     1381 ataccgggct cgtcaaagcc ccagaacgtg aattcgttgt actcttcgtg gggataaaac
     1441 agcacgtcca tgccgactgc gtccttggat ttctcgatga gtccgacatg gtaggcgatc
     1501 ttccgcggag aaaatccgag gaaataggat tccggcatgg tttcgagaat agccctgacg
     1561 ttacgcctgg agaccttgct cccgaggatc ctgaggactt cgtctatcac cctcttctgc
     1621 ctcgcgagcg ggtcttcccc tttgaagctc cccctctcga ggactttggc cgtccttatg
     1681 aagagctcct tcaggagcat gcccttccag ttggtccata cgtcgggacc gaccgacttg
     1741 atgtcggcga acgtcagcag atatagaagc gacagcgttt cgaggttctt tacggacttc
     1801 gcgaacctga ttacgaggct gtaatcgtgt atgtcccttc tctgcgagac atgggacatc
     1861 aggagatgat tttcgaccag gaattcgagc tgcttggtct cggtcgcgtc gagaccgagc
     1921 ctcccggcta tcttcgggat catggccgct cctttcgccg cgtggtcttt cccttccccc
     1981 ttgcccatat cgtgaaagag gcacgcgaga taaagcacat gcctgctgac gagtgactcc
     2041 gccgttttcg tcaggagagg gtgctccatc tcgtacttat agctgaggag gttctctatc
     2101 tccctcacca tgaaaatgga atggacatct accgtgtaaa catggtaagc gtcgtgctgt
     2161 accatgcata cgatctttcc gaactccggg atgtaatggc cgaggaaacg gagcctgttc
     2221 atctcgaata gagtgtcagc gacgttcttg cccttcttga gcagcctgag gaacgaagcg
     2281 ttcagctcgg ggttcgacct gacgccctca tccatcgtcg ttacactcac gttctctctg
     2341 atcagatcgt gcaggtaatt gctcattttt acctggtact tgtccgcgta ttcgaacgcc
     2401 ctcatcagat ttacagggtc atccctgaat atattgatgt tggaggcgga aagcatcccg
     2461 ccctgaatga tgaatccgtt gtcgagggtt actgtcttcg ccgtgcggat gccggactta
     2521 tggtccaaca ggcatttttc tatgagtctc ttcgactgct ccctcaactc gttcccgcgg
     2581 aggtaatata tcctcatgaa cctttccaca gcagggagct cggcgtcctt gaagcccaag
     2641 aacttggcga tcttttcctg aaattcaaag cccaatctgt cttctctccg ccccgccaag
     2701 taatggagct cggacctgat gagaagcaga aaattcaggc tcttttcgaa aatcctgatc
     2761 tcgttctcca tcaatattcc tttcggaagg aggtcggaaa agttcttgac cttgaacttg
     2821 gcctgggcga tccagagggc gtagtgaatg tcgcggagac cgccttcgcc ttccttaacg
     2881 tttggttcga gtaaatagac ggatctcccg aacctgttta tcctctgctc gttctccctg
     2941 atcttgctct gaatgaactt atgagaaatg gtggggagga gctggcagta tatcttctta
     3001 tccaggtcca tgtatagctg ttcaccgccg cacacgaacc tgctgtcgag gagcgaggta
     3061 agtatagtcg tgtcctcttc ctgcgagagc ccgatgcact cgtcgatcgt tctcacggag
     3121 tgtcccacgt cgagcccgag gtcccagagc aggtaaagga gcttctccgt cgcttccttc
     3181 gccagcgtct tgttgcgggg
//
LOCUS       DASSJH010000260         7507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1249268, whole genome shotgun sequence.
ACCESSION   DASSJH010000260 DASSJH010000000
VERSION     DASSJH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249268"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFJ67_05560"
     CDS             complement(<1..382)
                     /locus_tag="VFJ67_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7289888.1"
                     /translation="MFKELLKRKNISRISLEADTEQHGLKRVLGPLDLVSLGIGIIIG
                     AGIFVVTGQAAAQYAGPGISLSFVLSGFGCVLAGLCYAEMASMIPVSGSAYTYAYATL
                     GQFIAWVIGWDLVLEYLFASSTVSV"
     gene            complement(413..2536)
                     /locus_tag="VFJ67_05565"
     CDS             complement(413..2536)
                     /locus_tag="VFJ67_05565"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET7289889.1"
                     /translation="MEISSHTLILLVLGISVLSLAFAGYLAKYVLSSDMGTSAMQEIS
                     NAIKEGAEAFLKRQYSTIIMMSLALAALMFLVYWFLAGDKDTAWRTTVAFLFGAGCSA
                     LAGYIGMWISIRTNIRTASAVRSSLDRALRISFRGGAVSGITIVAMSLLGVGILFVLY
                     GGYSADAEELKRVPYLIVGYGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIPE
                     DDPRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVALFPHFGIKGILFPL
                     VARAFGLIASMIGISMVGLKNENEDPMGALNRGYYITCLLAAAGFFLATYLLLDGNLY
                     LFLAGVIGIATSIAFVYITQYYTEYRYRPVKFIAEASQTGPATNIIAGLAVGLECTAL
                     PVLTISAALVSAYYCGIYGLQEFSDISRNSAGLYGTAIACMGMLGTAAYILAMDTFGP
                     ITDNAGGIVEMSKQPEEIRNKTDRLDAVGNTTKALTKGYAIGSAALAAFLLFSAYMDE
                     VTHLTGKLFDTVDIAKVQVFVGGLLGAMLVFVFSALAIRAVARAAYYVISDVRAQFKE
                     NPGILQGTSKPNYARCVDIVTKGALKEMIPPGLLAVCMPIAVGLVFKQFGLGAETVAA
                     FLMVGTIAGILLATLMNNGGGAWDNAKKFVEMGNYGGKKSETHKATVVGDTVGDPFKD
                     TAGPSIHVLIKLLSTITLVLAPLFI"
     gene            2999..4828
                     /locus_tag="VFJ67_05570"
     CDS             2999..4828
                     /locus_tag="VFJ67_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HET7289890.1"
                     /translation="MTIHAMPLVVAALCVMVIFYRYYHGFIAAKVAVLDDSRKTPAYT
                     MYDGQNYFPTNKWVLFGHHFAAITGAGPLIGPVLAAQFGVLPGYLWILIGVVLAGAVH
                     DFIIFFASVRHGGKSLAEIAKSEISPLSGIVASIAVLFILVVALAGLGLAVVNALSES
                     SWGVFTIGCTVPIAFFMGLYMYKFRKGRIVEATAIGVVLLILAVYFGKDIAHSPLAPY
                     FSIQKTKITIFILVYGFIASILPVWLLLCPRDYLSSFMKIGTIVFLAAGIIVVNPELK
                     MPAVTEFIKGGGPIIPGALFPFTFITIACGAISGFHALVASGTSPKMVSKESDIKTIG
                     FVAMLIEGFIAILALIAATALFPGDYFAINVPGPIFETLGLSTVNLGHLSAQVGETLN
                     GRTGGAVSLAVGMAQIFTALPGMSHLMDYWYHFAIMFEALFILTTIDTGTRVARFMIQ
                     EFGGRFYKPFERVDHLPSAVISSALISCGWGYFVLTGSISTIWPMFGTANQLLAGIAL
                     CVGTTFIIKLGRAKYAWVTLVPMAFVMATTLTAAWLNIKNNYIPLTQKAGFTVQGYVN
                     IVFTVVMMICAVIIIIDSIRVWTKALPAERKEQAIPQKEAASD"
     gene            4896..5231
                     /locus_tag="VFJ67_05575"
     CDS             4896..5231
                     /locus_tag="VFJ67_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289891.1"
                     /translation="MGEHAKKEPSYRGIREAAGECGRLHRELRERYGEEEVEIIQIEP
                     KDYIYILPRVIKDIIKRREPLIPSIRTLLLWHPVPAVIVNGRVVGGMGKVPEAWEIYK
                     ELDKSSSRS"
     gene            complement(5228..6145)
                     /gene="secF"
                     /locus_tag="VFJ67_05580"
     CDS             complement(5228..6145)
                     /gene="secF"
                     /locus_tag="VFJ67_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET7289892.1"
                     /translation="MDLIRPGTHIDFVGKMGKAVILSTVLILISIGSLIYHKGPNWGV
                     EFTGGSEIQIKLTQDLSTERIKSTLGKAGYPPDEVQRIGLQGDNEYLIRFSPDLVKYE
                     QIQSFQKILEGIMSGNDEFKGGSILRVDYIGPKVGAELIEKAIIAVLLGCLGILIYVM
                     LRFEFAFALGAVLALVHDTVITLGAISITDKEFTLTIIAALLTIIGYSVNDTIIIFDR
                     IRENMKKGGRTTFEEIVNDSVNQTLSRTILTSTTVFIVLVALFVLGGSVIHDFAFALI
                     VGVIVGTYSSVFVASAIVIYWRKRKGLTL"
     gene            complement(6172..>7507)
                     /gene="secD"
                     /locus_tag="VFJ67_05585"
     CDS             complement(6172..>7507)
                     /gene="secD"
                     /locus_tag="VFJ67_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET7289893.1"
                     /translation="KGSDIKGEELKLQFFSKENLDTAAKIAKENFRDRADIDESDLSL
                     DFVLKGSYLNDIQVRAIDQVKQVIENRIQELGLIEPSIQKAESDRILIQVPGASERDR
                     QRIIDIIKRSAVLEFKIVKDTGAAEDVLLAKYGVKTPDELKSQNLMLIPGDTGTENEK
                     FFIATVDSPVTGESLSDARITFDEFGRPAVGFSFKGEGASKFGALTEKSIGQRLAIVL
                     DGKIKSAPTIQERITSQGRITGNFTSEEAKDLALVLRSGALPVPVTVEQERTVGPSLG
                     KDSIESGMLSMAVGGALVIIFMIFFYKLQGVVSDLALALNMLFIMGFLSAFGITLTLP
                     GIAGLVLTMGMAVDGNIIILERIKEELRAGKSPTAAIEAGYARSLWTVLDANITTLIT
                     ALILFWFGTGPIKGFAATVSIGIVSTVFSNVIVARVITNLIYQDRKVPALSI"
BASE COUNT         1678 a         2085 c         2034 g         1710 t
ORIGIN      
        1 ctacggatac ggtcgagctc gcgaagaggt actccagcac gaggtcccag ccgatcaccc
       61 acgctatgaa ctgcccgagg gtggcatacg cgtatgtgta ggcgctcccg gaaacgggga
      121 tcatggacgc catttcggca tagcaaagcc ctgcaaggac acatccgaaa cctgagagga
      181 cgaacgagag cgatattccg gggcctgcgt actgtgccgc ggcctgtccc gtgacgacga
      241 atataccggc accgattatg atcccgatgc cgaggctgac cagatcgagc ggaccgagca
      301 cacgttttag tccgtgttgc tcggtgtccg cttcaagcga tatccggctt atattttttc
      361 gcttcaggag ctccttaaac atcgcggtct cctgaatatt actcgctaac gtctagataa
      421 acaatggcgc gagaacgagc gtaatcgtcg agagcagctt tatgagcacg tggatggacg
      481 gccctgccgt atccttgaac gggtcgccca ccgtatctcc gacgaccgtc gctttgtgcg
      541 tctccgattt cttgccgccg taattcccca tctcgacgaa tttcttcgcg ttgtcccaag
      601 cgccgccgcc gttgttcatg agcgtcgcga gcaggatgcc ggctatcgtt cctaccatca
      661 ggaacgccgc cactgtctcg gccccgaggc cgaactgctt gaatacgagt ccgacggcga
      721 tgggcataca gaccgcgaga agaccgggag ggatcatctc cttgagcgcg cccttcgtca
      781 cgatatcgac gcacctggca tagttaggct tggaagtgcc ctggagtatg ccggggtttt
      841 ccttgaactg ggcgcgtacg tcactgataa cgtaatacgc ggcccttgcg accgccctta
      901 tagcgagcgc gctgaaaacg aacacgagca tcgcgcccag gagtccgccg acgaatacct
      961 gcaccttcgc gatgtcgacc gtgtcgaaga gctttccggt gaggtgggtt acttcgtcca
     1021 tgtaggcgga gaagagaagg aatgcagcga gcgccgcaga gccgatcgcg tatcccttcg
     1081 tgagggcttt ggtcgtattt ccgacggcgt cgagcctgtc ggtcttgttt ctgatttctt
     1141 cgggctgctt gctcatttcg actatgccgc ccgcgttgtc ggtgatcggg ccgaacgtat
     1201 ccatcgcgag gatgtaggcc gccgtaccca gcatgcccat acaggcgata gccgtgccgt
     1261 agaggcccgc cgagttccgg gagatatcgc tgaactcctg gagcccgtag ataccgcagt
     1321 aatacgcgct aacaagcgcg gccgaaatcg taaggacggg cagggctgta cattcgagac
     1381 ccaccgcgag accggctatg atgttcgtcg ccgggccagt ctgggaagct tcggctatga
     1441 atttcaccgg acggtacctg tattcggtgt agtactgtgt tatgtaaacg aacgcgatgc
     1501 tggtcgcgat accgatcacg cccgccagga acagatagag gttgccgtcg aggaggagat
     1561 acgtcgcgag aaagaatccg gccgccgcga gcaggcatgt gatgtagtat ccgcggttca
     1621 gcgcccccat cgggtcctcg ttttcgttct tgagcccgac catgcttatc ccgatcatac
     1681 tggcaataag accgaacgca cgggcaacga gcgggaagag tattcccttt attccgaagt
     1741 ggggaaagag cgccacgccc aggatcatcg cgcctatgtt ctcggctgct gtagattcga
     1801 agaggtcggc tccacggcct gcgcagtcgc cgacgttatc gccgacgagg tccgctataa
     1861 cagccgggtt ccgggggtcg tcttcgggta ttccggcctc gaccttaccc acgaggtctg
     1921 ctcccacgtc ggcagctttc gtgtaaatac cgccgccgag ctgtgcaaaa agggcgacga
     1981 agctcgctcc gaatccataa ccgactatga gataggggac cctcttgagc tcttcggcat
     2041 ccgcgctata accgccgtag agtacgaaga ggatgcctac gccgaggaga ctcattgcga
     2101 cgatcgttat gcccgatacg gcgcctcccc tgaacgaaat cctgagcgcc cggtcgagac
     2161 tcgacctcac ggcgctcgcc gtacgtatgt tcgtcctgat cgatatccac attcctatat
     2221 aaccggcaag cgctgagcac cccgctccga aaagaaacgc cacggtcgta cgccaggccg
     2281 tgtccttatc tccggcgagg aaccagtaaa cgagaaacat cagggcggca agagctagac
     2341 tcatcataat gatcgtcgag tactgcctct tgagaaacgc ctctgccccc tcctttatcg
     2401 cgttgctaat ctcctgcatc gcggaggtgc ccatatcgga gctgagcaca tattttgcga
     2461 ggtatccggc gaatgccaga gaaagcacac ttatacctaa aactaataaa attagcgtgt
     2521 ggctgcttat ctccatatca aaatctcctt tgttttatag acagcattga tttattttca
     2581 gtttattaat gactcgacgg gatatgaggg ataaacgggg gctatcgtaa tgaacgcggg
     2641 attgtctgta tataaaataa aaatatgagc ggatcataaa tttacggcag gcacactcat
     2701 atgagcggcc gcacctcaac caaggcggtc tatagcctca ttcctcccga agatgcggaa
     2761 aacatatcag aacgcataat aaagtcaagg agatcgggag ccggccggca aaacagagcg
     2821 taatgaagaa tatcggccga gattttgagt ttataattca tatactgtat cttcataatg
     2881 cggcgtattg aaactccatg tgaaatacag aaggcaagat tatggaacac ctcgtaaggc
     2941 tgcttccggt tatccggtgc gcgcctgaat attctaattc cggcatgagg ttagcgatat
     3001 gacgatacac gctatgcctc ttgtcgtagc ggctctctgc gtaatggtga ttttctacag
     3061 gtattaccac gggtttatcg ccgccaaggt cgccgtgctc gacgattcga ggaagacccc
     3121 tgcgtacacg atgtacgacg gccagaacta cttcccgacc aacaaatggg tgctcttcgg
     3181 gcatcatttc gcagccataa cgggcgcggg gcccctcatc gggcccgtac tggccgccca
     3241 gttcggagtg ctgccgggat acctctggat actcataggg gtggtgctgg cgggcgcggt
     3301 gcacgatttc atcatattct tcgcctccgt caggcacggc gggaagtccc tcgcggagat
     3361 agcgaagtcg gagatcagcc ccctttcggg gatcgtcgcg tcgatcgccg tcctgttcat
     3421 actcgttgtc gcgcttgcgg gactcgggct cgcggtcgtg aacgcccttt ctgaaagctc
     3481 gtggggcgtt ttcacgatcg gttgcacggt gccgatcgcg ttcttcatgg ggctctacat
     3541 gtacaagttc aggaaaggga ggatagtcga ggcgaccgca atcggcgtcg tcctcctcat
     3601 actggccgtc tatttcggga aggacatagc ccactccccg ctcgctccgt atttcagcat
     3661 ccagaaaacc aagattacga ttttcattct ggtctatggt ttcatagcct ccatactgcc
     3721 cgtatggctt ctgctatgcc cgcgggacta ccttagctcg ttcatgaaga tagggacgat
     3781 cgtgttcctc gcggctggga tcatcgtagt caaccccgag ctcaagatgc cggccgtgac
     3841 cgagttcata aagggcgggg ggccgataat tccgggcgcg ctgttcccgt tcacattcat
     3901 aacgatcgcc tgcggcgcta tatccggctt ccacgcgctc gtcgcctcgg gcacgtcccc
     3961 caagatggtc tctaaggaat ccgacatcaa gacgatcggg ttcgttgcga tgctcattga
     4021 agggttcatc gcaatactcg cccttatagc cgcgacggct ctattccccg gcgattactt
     4081 cgccataaac gtacccgggc cgatattcga gacgctcgga ctgagcaccg taaacctcgg
     4141 ccacctttcg gcgcaggtcg gagagacctt gaacgggagg accggcgggg cggtctcgct
     4201 tgctgtcggt atggcccaga tattcacggc ccttcccggc atgagccacc tgatggacta
     4261 ctggtaccac ttcgcaataa tgttcgaggc gctcttcata ctcacgacca tagacacggg
     4321 caccagggtc gcccggttca tgatacagga attcggcggg aggttttaca agcctttcga
     4381 gagggtggac cacctgccct cggccgtgat atcgagcgcg ctcatatcat gcgggtgggg
     4441 atatttcgta ctgacgggga gcatcagcac tatctggccc atgttcggga cggctaatca
     4501 gctcctggcg ggtatcgcgc tctgcgtcgg gacgacgttc ataatcaagc tcgggagggc
     4561 aaaatacgcg tgggtaacat tagtgcccat ggccttcgta atggcgacaa cgctcaccgc
     4621 tgcgtggctg aatataaaaa acaattacat accgctcacg cagaaagccg gattcacagt
     4681 ccaggggtac gtgaacattg tattcacggt cgtcatgatg atatgcgcgg tcataatcat
     4741 catagactcc ataagggtat ggaccaaggc gctcccggct gagcgcaagg agcaggcgat
     4801 ccctcaaaag gaggcggcca gcgattagcg aatcaaccgt tatcatagtc agggagagcg
     4861 atactggaag cggatgcgta tgctgctcgg gcggcatggg agagcacgcg aaaaaggagc
     4921 cctcttaccg cgggataagg gaagccgctg gggagtgcgg gaggctccac agggagctgc
     4981 gcgagaggta cggggaggag gaagtggaga taatacagat cgagcccaaa gactacatat
     5041 acatcctgcc aagggtgatc aaggacataa tcaaacggcg ggagcccctg ataccgagta
     5101 taaggacttt gctgctctgg catcccgtgc ctgcagtcat cgtaaacggc agggttgtcg
     5161 gaggaatggg taaagttccc gaagcctggg agatttataa ggagcttgac aagagctcgt
     5221 cacgttccta aagcgttaat ccctttctct ttctccagta tataactatc gcgctcgcaa
     5281 cgaatacgga tgaatatgta cctacgataa caccgacaat aagggcaaac gcgaagtcgt
     5341 gtatgaccga gccccccaga acaaagagag ccactagcac gatgaatacg gtggtactcg
     5401 taaggatcgt cctcgagagc gtctggttta ccgaatcgtt cactatctct tcgaatgtag
     5461 ttcttccgcc cttcttcata ttctccctta tcctgtcgaa tatgattatg gtgtcattca
     5521 ccgaataacc gattatcgtc agaagcgccg ctatgatagt aagagtaaat tccttgtccg
     5581 tgatggaaat cgctccgaga gtgatgaccg tatcatgaac cagcgcaagc acggccccca
     5641 gagcgaaagc gaattcgaac ctcagcatta cgtatataag tatccccaga cacccgagca
     5701 ggacggctat tatagccttt tctataagct cggcgccgac cttggggcct atgtagtcca
     5761 ccctcagaat ggagccgccc ttgaattcgt cgttaccgct catgatgcct tcgagaatct
     5821 tctggaaaga ctgtatctgc tcgtatttaa ccagatcggg ggaaaatctt ataagatatt
     5881 cgttgtctcc ctgaagaccg attctctgta cctcatcggg cgggtagccc gcctttccca
     5941 gggtgctctt tatccgttca gtgctgaggt cctgagtgag ctttatttga atttcggagc
     6001 cgccggtaaa ttccacgccc cagttgggac ctttgtgata tatgagcgag ccgatcgaga
     6061 tgagtatcag aacggtcgag agaataaccg ccttccccat tttccccacg aaatctatat
     6121 gagttccagg tcttattaaa tccaagttgc aatatcctcc cgagtcttcc ttcagatgct
     6181 taaagcaggg actttcctgt cctgatagat caaattcgtg attacacggg caacgattac
     6241 gttgctgaat acggtagata caataccgat cgaaacggtt gcggcgaaac cttttatcgg
     6301 accggtcccg aaccagaaaa gaatcaatgc ggtaatgagc gtcgttatat tcgcgtcgag
     6361 caccgtccag agagacctcg catagcccgc ctctatcgca gccgtagggg atttgcccgc
     6421 cctgagctcc tcttttatcc tctcgagaat gatgatattc ccgtcaacag ccatacccat
     6481 cgtgagtacc agtcccgcta taccgggcag agtgagcgtt atgccgaatg cggatagaaa
     6541 tcccatgata aacagcatgt tcaaagcgag ggcgaggtcc gagacaacac cctgaagctt
     6601 gtagaagaag atcatgaata ttattaccag cgctcctccg acggccatcg aaagcatccc
     6661 gctctcgata gagtccttgc cgagcgaggg gcctacagtc cgctcctgct ccacagttac
     6721 gggcaccggc agtgcgcccg agcgtaggac gagcgcgagg tctttcgctt cctcgctcgt
     6781 gaaattgccc gtgatccttc cctgtgaagt tatcctctcc tgtatcgtgg gcgctgattt
     6841 gatcttgccg tcgagcacga tcgcgagcct ttgtccgatg ctcttttcgg tgagcgcgcc
     6901 gaacttgctc gccccctccc ccttgaagct gaagccgacg gcgggcctgc cgaattcatc
     6961 gaatgtgatc ctcgcgtcgg aaagcgactc gcccgtgacg ggagaatcca cggtcgcgat
     7021 aaagaacttc tcgttctccg tgccggtatc tcccgggatg agcatgaggt tctgggactt
     7081 gagctcgtcg ggcgtcttaa caccgtattt ggccagcaat acgtcctctg ccgctcccgt
     7141 atccttgact atcttgaact cgagcaccgc ggagcgtttt attatgtcga taatcctctg
     7201 tctgtccctt tcggacgcgc ccgggacctg gatcagtatc ctgtccgatt cggctttttg
     7261 aatcgagggc tcgatgagac cgagctcctg gatgcggttt tcaatgacct gcttcacctg
     7321 gtctatagct cttacctgta tgtcgttcag gtacgagcct ttgagcacaa aatctaaaga
     7381 caggtcggac tcgtctatgt cggccctatc cctgaaattc tcttttgcga tctttgcggc
     7441 ggtgtcgagg ttctctttgg aaaagaattg cagtttgagc tcctctccct tgatgtcgga
     7501 accctta
//
LOCUS       DASSJH010000261         6319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_600031, whole genome shotgun sequence.
ACCESSION   DASSJH010000261 DASSJH010000000
VERSION     DASSJH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600031"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1802)
                     /locus_tag="VFJ67_05590"
     CDS             complement(81..1802)
                     /locus_tag="VFJ67_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289894.1"
                     /translation="MTKYTRVRFLLVYALFIIIGLGNIGGCETSEDGSGGDDDTELRT
                     CDLTGADSACEICAELGDAACCTLTSPAGCGSVSAITTFTTCNDDLSAACVIGTEPTP
                     VPTPVPTPEPTPEPTPDPTGVPTPVPTPVPTPTPAPTPTPGPTPTPLPCPSPGPAGTH
                     TISIINQCSQSVWIGAQSINFTSLLCEAGTGSSPAVGNCGPEGGQGTTIANNTNWVGA
                     PPWDTDGGATWEIPPSDNDPAGLGGVRQLTIPDCWDSGILVARTGCQVLPSPAPTTNL
                     TCETGNCGGTYGVENCGGINGPKPASIAEFTFDGGIPANCTGADNYDVSFVAGFNVLI
                     ETAPCQNGCGSAGSGCTQMPVCPWDTYVFQPDTQPNVGGFVVTPNGDIGTCLSPFNMA
                     SIGLPPLENMSADQMNRLGCVGGYDETPWTNNDAWGVCMTNFGSNTDSCAMTSSTTCG
                     TNMNIPPTGNSTPPCCQFPTSMCDPYGQCDSVMGRTAAWPTDNDGGSPTEYIENVHEA
                     CGDNTSGGQAGAYAWSFDDGDQVLGLANALFTCQGDDINYVVTLCPDSAPQPTPQPTL
                     VPAATEE"
     gene            2048..2485
                     /locus_tag="VFJ67_05595"
     CDS             2048..2485
                     /locus_tag="VFJ67_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7289895.1"
                     /translation="MKITSPDFGEGGMMPREMTCEGRDVSPELHITGVPEGAKSLALI
                     CDDPDAPGGMFVHWLVWNIPPDTKKIERGKEPDGVQGVNGFGGRGYRGPCPPSGTHRY
                     FFRIFALKDNLNLRDGSKKQALKEAMDPYIIAKAELMGKYRRG"
     gene            2871..3332
                     /locus_tag="VFJ67_05600"
     CDS             2871..3332
                     /locus_tag="VFJ67_05600"
                     /inference="COORDINATES: protein motif:HMM:NF037752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7289896.1"
                     /translation="MKGYSILILLALLLFSDFAFSQTCPCDTLELSDGTTGNEIIELI
                     CPGGELGEGTLFELSQFSVAVGTESAFYQALNLPDLGSGCLIGLAGVGTDGIELTPEE
                     TEICRQSLIQRCDLKLITPIPTLSEWGMIATAGVLGIIGLIVAARRRKAAA"
     gene            complement(3553..4122)
                     /gene="def"
                     /locus_tag="VFJ67_05605"
     CDS             complement(3553..4122)
                     /gene="def"
                     /locus_tag="VFJ67_05605"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7289897.1"
                     /translation="MAFRKILTYPDPFLRKKCARVEEIDDGVLGILDDMAETMYEARG
                     VGLAASQIGVDKRIVVVDISPRKTGAEDEDEEGVVEYEGPGLLELINPEIISSEGEII
                     AEEACLSIPGFTSDVKRKKKVTIEFCNREGDLMEIEAEDLLARVFQHEIDHLNGVLFI
                     DRLSRLKRELIKRKIEKEFGKEEKRYAVL"
     gene            complement(4253..4537)
                     /locus_tag="VFJ67_05610"
     CDS             complement(4253..4537)
                     /locus_tag="VFJ67_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7289898.1"
                     /translation="MKRSDIEKELADKFNLQPSQSEQILDTIIEHMTEVLEGGGRIEI
                     RGFGSFFTKSYGSYEGRNPRTGSKVMVSSKKLPHFRPSRELITKLNGGSE"
     gene            complement(4708..6126)
                     /locus_tag="VFJ67_05615"
     CDS             complement(4708..6126)
                     /locus_tag="VFJ67_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289899.1"
                     /translation="MAKSGSKKGGNGKGNGKFSLGERYEQALNYPLFDSIFNRRSRRF
                     GLGMKLEDTTLGFESQYDPIPLEEMEEALLVWAGTGLTGLCLADLPPETGIDLLCQWT
                     GRTWPSACNNHGTELFFTNDKGLYHVNVKNMLPKNHELDMFFRMSREKRMERILELYR
                     ESLVKLEEGRAQLPDRMPGLFEFNQWNTNKPGTSVFIPVTDVTEEYINLLHLYCSYTY
                     GFTVIDDLTGKPAGVQKWIDKGRLKGDVKMSLFDLEVRILTGLNVEQAFICQNMSLAL
                     QALGLAGWTFSGFIPRFAMGAAPHLFKGLGFRFIQPRKGVIDPPATVPVGRDGVFHAY
                     CPPYYKDMDDAIDAFHHHKEAAWKPDKPFPYTEPDKHLMRAPRPTENTIQIVKDVANY
                     IYDTYGRFPAFVDPMYMRLVFQAMNIDVEFYDKYYPKGSYSEMHLNKFLSDHPEARGS
                     VGEKAGVREARAEGAAKKKIKA"
BASE COUNT         1325 a         1760 c         1774 g         1460 t
ORIGIN      
        1 cctagaggcc cgagccaatg ccgggctttc cacagcccgg cattgattcg gattttattt
       61 agcgtctcgc ctttaattta tcattcctca gtcgccgcgg gaacgagagt cggctgcggt
      121 gtgggctggg gcgcgctgtc cgggcagagg gtcacgacgt aatttatatc gtcgccctga
      181 caggtaaaga gcgcgttcgc gagaccgagc acctggtcgc cgtcgtcgaa tgaccatgcg
      241 taagcgcccg cctgtccccc gctcgtgttg tcgccgcacg cttcgtgcac gttctcgatg
      301 tattccgtag gactcccccc gtcgttgtct gtaggccacg cggcggtcct tcccatgacc
      361 gagtcgcact ggccgtacgg gtcgcacatt gaagtcggga actggcagca gggcggggtc
      421 gagttaccgg taggcggtat gttcatatta gttccgcacg ttgtggaaga cgtcatcgcg
      481 cagctgtcgg tgttgctgcc gaaattcgtc atgcagacgc cccaggcgtc gttgttcgtc
      541 cagggggttt catcgtagcc acctacgcat ccgagcctgt tcatctggtc cgcagacata
      601 ttttcgagtg gtggaagtcc gatcgaggcc atgttgaacg gcgagaggca cgtccctatg
      661 tctccattgg gagtcaccac gaagccgcct acattcggct gggtgtccgg ctggaaaacg
      721 tacgtgtccc acgggcacac gggcatctgt gtacaccccg acccggcgct cccgcagccg
      781 ttctgacagg gcgcggtctc gatcagcacg ttgaaccccg cgacgaatga gacgtcgtag
      841 ttgtctgccc cggtgcagtt agccggaata ccgccgtcga acgtgaactc cgcgatggag
      901 gcgggcttgg gtccgttgat cccgccgcag ttctcaaccc cgtacgttcc gccgcagttg
      961 ccggtttcgc acgtgaggtt cgttgtagga gcaggagacg ggagtacctg gcagcctgtg
     1021 cgggctacga gaattcccga atcccagcag tcgggtatag tcagctgcct cacgccgcca
     1081 agccccgccg gatcgttatc gctgggcggt atctcccatg tggcgccgcc gtcggtgtcc
     1141 cagggcgggg cccctaccca attggtattg ttggcaatag tagtgccctg tcctccctcg
     1201 gggccgcagt tgcctacagc gggcgagctg ccggttccag cctcgcatag gagcgaggtg
     1261 aagttgatgc tttgcgcgcc tatccatacg gactgcgagc attggttaat aatgcttata
     1321 gtgtgagttc cggccggtcc gggtgagggg cacggcagag gcgtgggggt cggtccggga
     1381 gtcggcgtcg gagcgggcgt cggtgtcggc acaggcgtgg gcaccggagt cggcacaccc
     1441 gtcggatcag gcgtcggttc cggcgtgggc tccggtgtgg gaacgggggt cggcactggc
     1501 gtaggctccg ttcctatgac gcacgcggcg ctaagatcgt cgttgcacgt agtaaaagtg
     1561 gttatcgccg aaacggatcc gcagccggcc ggggatgtca gcgtacagca ggccgcatcg
     1621 ccgagctcgg cgcaaatctc gcaggcggaa tcggcgcctg tcagatcgca cgtccgtaat
     1681 tcggtgtcgt cgtcaccgcc cgagccgtcc tcggacgttt cgcatccgcc gatgttgccg
     1741 aggccgatta ttataaaaag agcgtaaacg agaagaaacc gaacgcgagt gtatttggtc
     1801 attttatccc cttcaaagcg cacgaatttt taaaagcctt tacgttttta taattcaaat
     1861 ctcaacgttt acgtaaaaat acggccgttc gtgatttagc acaatatata aaatattaaa
     1921 ttgttgttcg tcgcggccga gaccggtccg cacctgcggg atcgcggaga gatacgcata
     1981 actccttgtc aacgatatac aaaccgtgag ataattaatc tagcgaaaat cttgcaggag
     2041 gcggattatg aagataacga gccccgattt cggagaagga gggatgatgc cgagggagat
     2101 gacatgcgag ggacgggacg tatccccgga gcttcacata acgggagtcc ccgaaggggc
     2161 gaaatcgctc gcgctgatat gcgacgatcc cgacgcgccg ggcgggatgt tcgtccattg
     2221 gctcgtctgg aatatccctc ccgatacaaa gaagatagag cgcgggaagg agcctgatgg
     2281 cgttcagggg gtaaacggct tcggcgggag gggctacagg gggccctgcc ctccttcggg
     2341 cacgcacagg tacttctttc gaatcttcgc tctcaaagac aatctcaatc tcagggatgg
     2401 ctcaaagaag caggcgctca aggaagcgat ggacccatac atcatcgcca aggccgagct
     2461 gatggggaaa tacaggagag gctagcgcag aacctgcgct cgtatacggt tcttccgtat
     2521 ctcttctact tgaaccggca actgcggcaa tctcacccct tcctgtcatt tcgagggagc
     2581 gtagtatgcg accgcggcaa tctcatgaat aaagcgagat cgccacgacc ataaagcggt
     2641 ctcgcgaaga cagaaaatga ggtgtcccct caccctcatc ctacactcca acaaccgccc
     2701 ggagtatata cccctgcgga gttgtcggaa tgtcggaaaa taaaaaatcc tccataacca
     2761 tcgaccaccc ggtgaataac tcaattgtat ttgaaacagc cctgttttat aatagtgctg
     2821 attaaaattc gtatatacca atctaattaa aggaggagga ggaaaaagag atgaaaggct
     2881 acagcatctt aatcctactg gctttattgc ttttttccga ttttgctttt tcacaaacct
     2941 gtccgtgtga tactctggaa ttgagcgacg gcaccaccgg caacgaaatt attgagctga
     3001 tctgccccgg aggcgagctt ggcgaaggca cacttttcga attaagtcag ttttcagtcg
     3061 cggttggtac tgaatccgcg ttttatcagg cgctaaactt acccgatctt ggatccggtt
     3121 gtttaatagg cttagcaggc gtaggcaccg atgggataga gcttaccccg gaagaaaccg
     3181 agatctgcag gcagagcctg atacaaagat gcgacttaaa gctaattact cccatcccca
     3241 ccctcagcga atggggaatg atagcaacgg ccggggtttt gggaataatc gggttaatcg
     3301 tcgctgcgcg cagaagaaaa gcggccgctt aaacaaagga ttcaagcgta aaaaaataac
     3361 gtaaaggaaa gggagcttcg gctccctttt tgtttccttc ccgtcattcc agaaacgatt
     3421 gcgagcgaca aatactagca agctgaatcg cgggtggaag ccgctcctac agacaggttg
     3481 caagatatat gatgcagtat gtaaaacgag accctgaatc aagttcaggg tgacagatgg
     3541 cagggacacc cctcacagca cggcgtacct cttttcctct ttcccgaact ctttctcgat
     3601 cttgcgcttt atgagctcgc gcttgagacg cgagagcctg tcgatgaaaa gcactccgtt
     3661 caggtggtcg atctcgtgct ggaatacgcg cgcgagcagg tcctcggcct cgatctccat
     3721 caggtccccc tcacggttgc agaactctat cgtcaccttc ttcttcctct tcacgtcgct
     3781 cgtaaacccg gggatgctga ggcacgcctc ctcggcgatt atctctccct ctgatgaaat
     3841 gatctcaggg tttatgagct cgaggagccc gggcccttcg tattccacga ccccttcctc
     3901 gtcctcatcc tcggcgcccg ttttcctcgg gcttatatcg acgacaacta tcctcttatc
     3961 gacgcctatc tgggacgcgg caagccccac tccgcgggcc tcgtacatcg tctccgccat
     4021 gtcgtctaag atgccgagca ccccgtcgtc gatctcttcg acacgggcgc atttctttct
     4081 caggaacggg tcgggatacg taagtatttt tcggaacgcc ataaaaatag gctaaaggat
     4141 acggccggaa tgtcaatgtg cggattaagc gcttgagaac gggacccggg cggtatgccc
     4201 tgtccttact cgatatatct cgggaacgcc ggatgagggc gatgcgccga agctattcgc
     4261 ttccgccgtt cagcttcgtg atgagctctc tgctcggcct gaaatgaggg agcttcttcg
     4321 acgataccat cactttggaa ccggtcctcg ggttccgccc ctcgtaagaa ccgtagctct
     4381 tggtgaagaa gcttccgaac cccctgatct cgatcctgcc gccgccctcg agcacctcgg
     4441 tcatatgctc gattatggta tcgagaatct gctcggattg cgaaggttga agattgaatt
     4501 tgtccgccaa ttccttttct atatctgaac gcttcatagt taagctccca acctttttag
     4561 tccaccatta ctcccgcccc tggcctacaa ttccaattta gataattctt aagtatttgt
     4621 caagtgttag attgaccggg aatgaaatta atttgactcc ccctataacc gggcataagg
     4681 cgtccgaagg acttgtttgc tattgaatta agcctttatc ttctttttcg cggccccctc
     4741 cgccctcgcc tccctgactc ccgccttttc cccgacgctg cccctggcct ccgggtggtc
     4801 gctcaggaat ttgttcaggt gcatctcgga gtaggagccc ttggggtaat acttgtcgta
     4861 gaattcgacg tctatgttca tcgcctgaaa aacgagcctc atgtacatcg gatcgacgaa
     4921 cgcggggaac ctgccgtacg tgtcgtaaat gtagttggct acgtccttca ctatctgtat
     4981 cgtgttctcc gtcggcctcg gcgctcgcat caagtgcttg tccggctccg tgtatgggaa
     5041 gggcttgtcc ggtttccacg ccgcttcctt gtgatgatgg aacgcgtcta tagcatcgtc
     5101 catgtccttg taatagggcg gacagtaggc gtggaacacg ccgtcccttc cgacgggtac
     5161 ggtcgcgggc gggtcaatga cgccctttct gggctgtatg aatctgaagc cgagcccctt
     5221 gaacaggtgc ggtgccgccc ccatggcgaa cctcgggatg aacccgctga atgtccatcc
     5281 ggcgagtccg agcgcctgga gagcgaggct catgttctgg catatgaaag cctgctctac
     5341 gttcagcccg gtgagtatcc tgacctcgag gtcgaagaga gacatcttga cgtccccctt
     5401 gagcctccct ttgtctatcc acttctgcac gcccgccggc tttccggtga ggtcgtcgat
     5461 tacggtgaac ccatacgtgt agctgcaata gaggtggagg aggttgatgt attcctcggt
     5521 aacgtccgta acggggatga atacggacgt ccccggcttg ttcgtattcc actgattgaa
     5581 ttcgaagagt cccggcatcc ggtcggggag ctgcgccctc ccttcctcga gcttcacgag
     5641 gctctcccgg tagagctcga gtatcctctc catccgcttt tcacggctca tgcggaagaa
     5701 catatcgagc tcgtggttct tgggaagcat gttctttaca tttacgtggt aaagcccttt
     5761 gtcgttcgtg aaaaagagct cggtcccgtg gttgttgcac gccgacggcc acgtgcgccc
     5821 cgtccactgg cagaggaggt ctatgcccgt ttcgggaggg agatcggcga ggcagaggcc
     5881 cgtgagcccg gtgccggccc acacgaggag cgcctcttcc atctcttcga gcggtatggg
     5941 gtcgtattgg gactcgaagc cgagcgtcgt atcctcgagc ttcatcccca gtccgaagcg
     6001 ccgggagcgc ctgttgaata tggaatcgaa aagcgggtaa ttgagggcct gctcgtacct
     6061 ctcgccgaga gagaacttgc cgttcccttt tccgttcccg ccctttttgc tgccgctttt
     6121 agccataata cccctccgga tagtgataaa taaatattaa gaggatagtg acccccccag
     6181 atagagactt taaaataact cggcggaaaa tgcaaggaac cgggggtacg ggtgagtgag
     6241 tcagcgttta tgcgcggctt cggacgcgga gatcgaaccc agcgggagac tcaggggaag
     6301 gtctggaaat ctaatgaaa
//
LOCUS       DASSJH010000262         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_280390, whole genome shotgun sequence.
ACCESSION   DASSJH010000262 DASSJH010000000
VERSION     DASSJH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280390"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..823
                     /gene="trmB"
                     /locus_tag="VFJ67_05620"
     CDS             170..823
                     /gene="trmB"
                     /locus_tag="VFJ67_05620"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET7289900.1"
                     /translation="MDNSEIVNPILDISLRTLPLDLGKVFGNANECLLEIGFGDGDFL
                     IENALGNPGKNYVGIEIKKSRFNKAVKRAKRQKAGNVRFIHMDADIAAGELFGPDTFT
                     TAYINFPDPWPKDRHKKHRIINRGFLTKLSRIMKKDAILEMASDHSEYVTNTLYTMRE
                     TGLFETAFPDPGYTLQMPGRRETKYEAEFRSEGREIRYMSFKNIKRPGFSRSRSGKI"
     gene            855..1130
                     /locus_tag="VFJ67_05625"
     CDS             855..1130
                     /locus_tag="VFJ67_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289901.1"
                     /translation="MRKILTVCAIIYTGLFLSGCSEYGPGYLFQLIFIVAPLVALGVM
                     LLKRTESTNDSLYILEGQIKRLSSKLESLEAKVTELTDKENKSKVKK"
     gene            complement(1136..1741)
                     /locus_tag="VFJ67_05630"
     CDS             complement(1136..1741)
                     /locus_tag="VFJ67_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289902.1"
                     /translation="MNRFIILAICVTGSVILVSAGSALSQSAAPPAPAPAPAPVPADF
                     PSYIVTPSEDLNPYDLSTMQKSTETNYRDGTGTLGTSVERQSNIEVNEALEKKKQERL
                     KAEADAKAKEAKAASESETGEAIPQEETSVAEPPQLGESEKDLETPGVRSGLFTWTDE
                     QGVLHATNDIGQVPIKYQVEALQNSSKNINLNKGPQDKKQR"
     gene            complement(1891..2433)
                     /locus_tag="VFJ67_05635"
     CDS             complement(1891..2433)
                     /locus_tag="VFJ67_05635"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7289903.1"
                     /translation="MIKKWKVIGSERLSSNRVFSTRKDRSVSPITGEEHDFFVIEAPD
                     WVNVVAVSDENEMLLIRQYRHGIGSETVEIPGGCVDPGETPMDAARRELLEETGYVSD
                     EWTCIGEVVPNPAIQNNTCYTFLAEGLRKEREPNFDTTEYIVTFTVPVRDIPRLVAER
                     QITHSLVVSAFYWYCLHAAG"
     gene            complement(2467..>2959)
                     /locus_tag="VFJ67_05640"
     CDS             complement(2467..>2959)
                     /locus_tag="VFJ67_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7289904.1"
                     /translation="RIFVFPKPVIAALNGHTVAGGCILAIACDSRIMVSGKPRIALNE
                     LTIGASVFTSIAEILKFTVGSRNAQAILYSGRMSSAEEALGLGLVDEAHPAEDFGDAV
                     LACARDLAGRNGEAFGSIKRLLRKHVIDNIERDEINTVSDFVDIWYSPDTREKLKKIE
                     IRG"
BASE COUNT          720 a          806 c          752 g          681 t
ORIGIN      
        1 gctcgacaaa aaagcgcgcg gcgggaagat cgagatggcg ctccccggat ccctgggccg
       61 tatggccgag atagacgggg gttacgggct caagatagat gaatccctca tccgggaaag
      121 cacccggtaa aacccgttca taatcacagc cacgtctgac cgtaaaaaaa tggacaactc
      181 cgaaatcgta aatccgatac tcgatatatc gctccgcacc ctgccgctcg atttgggcaa
      241 agtgttcggg aacgcgaatg aatgcctcct cgagataggt ttcggggacg gggacttcct
      301 cattgagaac gcgctcggga accccgggaa aaactacgtc ggaatagaga taaagaagag
      361 caggtttaac aaggccgtca agcgggcgaa gcgccagaag gcagggaacg tcaggtttat
      421 tcatatggac gcggatatag cagccgggga attgttcggc ccggatacat ttacgaccgc
      481 ctatatcaac ttccccgacc cgtggcctaa ggacaggcac aagaaacaca ggataataaa
      541 ccgcggcttc ctcacaaagc tttcccgtat aatgaaaaaa gacgcaattc tcgagatggc
      601 gagcgaccac agtgaatacg tcacgaatac cctctatact atgagagaaa cgggcctgtt
      661 cgagacggcg ttccccgacc ccggttatac attgcaaatg cccgggcgca gggaaacgaa
      721 atacgaagcg gaattcagat cggaagggag ggaaatccgc tacatgagtt ttaaaaacat
      781 aaagcgtccc gggttttcaa ggagccgctc aggtaaaata tgagtgcaaa atcaataagc
      841 aggaggatag aagaatgaga aaaatactga cagtgtgcgc aattatatat acggggttgt
      901 tcctgagcgg ctgctcggaa tacggacccg gatacctgtt tcagctcata tttatagtag
      961 cccctctcgt cgcgctcggt gtaatgctcc tgaaaaggac cgagagcacg aacgactccc
     1021 tctacattct ggaaggacag ataaagagac tctcctccaa gctcgagagc ctggaagcta
     1081 aagtgaccga gcttactgac aaggagaata aatcaaaggt caagaaatag acggcttatc
     1141 gctgcttctt atcctgggga cctttattca gattgatgtt ctttgaagaa ttctggagag
     1201 cctcgacctg atattttatc gggacctggc ctatgtcgtt ggtagcgtga agcacgccct
     1261 gctcgtcggt ccacgtaaaa agacctgacc ttacaccggg cgtttcgaga tccttttcgg
     1321 actcgcccag ctgcggaggt tccgcgacac tcgtctcctc ctgaggtatg gcttccccgg
     1381 tctccgactc actcgccgcc ttcgcctctt tcgccttggc atccgcctcc gctttcaacc
     1441 tttcctgctt cttcttctcg agcgcttcgt tcacttctat attgctctgg cgctcgacag
     1501 acgtaccaag cgtgccggtg ccgtccctgt aattggtctc ggtgcttttc tgcatcgtcg
     1561 agagatcgta gggattgagg tcctcgctgg gcgtaactat atacgaaggg aaatcggcgg
     1621 gtacaggggc aggagcgggt gcaggagccg gaggcgcggc cgactgagat aatgctgacc
     1681 ctgcggatac gagaattacg ctcccggtca cgcatattgc cagtattata aatcggttca
     1741 tattcggcct cctctttcag gcaccctctt ataaatatac acttgcttga gaaaatactc
     1801 aaacccaaaa attaatgtat agcggagacc gaagccaata gttaaaccat tttattaaga
     1861 aagatttata gtgggaggtc aggtatcaaa tcacccggct gcatggagac agtaccagta
     1921 aaacgccgaa accactaacg aatgcgttat ttgcctttcg gccactagtc tcggtatgtc
     1981 ccgcacaggt acagtgaacg taacaatgta ttcggtcgta tcgaagttag gctccctctc
     2041 cttccggagg ccctcagcga ggaaggtata gcatgtattg ttctgtatag ccgggttagg
     2101 cactacctcg cctatgcacg tccattcatc cgacacgtag cctgtctctt cgaggagctc
     2161 cctcctggcc gcgtccattg gtgtctcccc cggatcgacg catccccccg gtatctcgac
     2221 cgtctccgac ccgatgccgt gcctgtactg ccttatcagc agcatctcgt tctcatccga
     2281 aaccgcgacc acgtttaccc agtcaggggc ctctatgacg aaaaaatcgt gctcttcccc
     2341 ggttatcggg gatacgctcc tgtcctttct cgtcgaaaat accctgtttg aggagagacg
     2401 ttcagagccg atcaccttcc atttctttat catagccact ctgtcacctt gaatacgcca
     2461 gcgcacttaa ccgcggattt caatcttctt taatttctcc cgggtatcgg gagagtacca
     2521 aatatcgacg aaatctgaaa cagtgtttat ttcgtcccgc tcgatgttat cgatgacgtg
     2581 tttcctgagt agtctcttta tacttccgaa ggcctccccg tttcttccgg cgaggtcacg
     2641 ggcgcaggcg agcacggcat cgccgaaatc ttcggccgga tgtgcctcat cgactagacc
     2701 gaggccgagc gcttcctcgg cggagctcat cctcccggaa tatagaatcg cctgggcgtt
     2761 cctggaaccg accgtgaatt taagtatctc cgcgatgctc gtaaataccg aggcgccgat
     2821 cgtcagctcg ttgagcgcta tcctgggctt ccccgagacc atgatcctgc tgtcgcaggc
     2881 gattgcgaga atgcagcccc cggcgacagt atgcccgttc agggctgcga tcacgggttt
     2941 tggaaagacg aatatgcgc
//
LOCUS       DASSJH010000263         4900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_924007, whole genome shotgun sequence.
ACCESSION   DASSJH010000263 DASSJH010000000
VERSION     DASSJH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924007"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFJ67_05645"
     CDS             <1..197
                     /locus_tag="VFJ67_05645"
                     /inference="COORDINATES: protein motif:HMM:NF016718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289905.1"
                     /translation="VDIESKRFFFDVKENHKGKYLRITELSGGRSCIVIPLGGINLFK
                     ERLGEVIQEAEKLIDTNGSM"
     gene            complement(363..1625)
                     /gene="lysA"
                     /locus_tag="VFJ67_05650"
     CDS             complement(363..1625)
                     /gene="lysA"
                     /locus_tag="VFJ67_05650"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7289906.1"
                     /translation="MHDSYQYRDGEFFIEEVPARKIAESVGTPCYIYSCAVIEDAFLT
                     YKEAFFGIDTLMCYSVKANSNLAILNSFARLGAGFDIVSLGELIRVRRAGGDPSKVVF
                     SGVGKTEEEMKAAIEAGILFFNVESVEELRLLNEVGVRLGTRAPVALRVNPDIDPKTH
                     PYISTGFKKSKFGIEISRAFEVYKEAAGMKGIRVTAIDAHIGSQIFELTPFVDSLKKL
                     VKLADDLLSNGIGIEYIDIGGGLGISYKMDEIPPHPKEYAQAIKEAFGCKPYKLVLEP
                     GRSLVGNAGVLVTKVLYLKEGTAKKFVIVDAAMNDLIRPAFYESYHEILTVSGPAKAK
                     DKVDIVGPICESGDFLAKDRKFPAVSSGDLLAVMSAGAYGFAMSSNYNTRPRVPEVLV
                     KGGEFRVVRKRETYDEMLSGESVPDFGG"
     gene            complement(1739..2332)
                     /locus_tag="VFJ67_05655"
     CDS             complement(1739..2332)
                     /locus_tag="VFJ67_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HET7289907.1"
                     /translation="MGKKVEFYYDFTSPYTYIASSRIGKICKDNGAELEWKPFFMGGL
                     FIETGVKAPVDIANKFAYVKHDTQDLARHYGVDFKFPSVFPLHSVKAMRGAFAAAEKG
                     KLTEYTHEMFRLYWTEGRDLSKDDELRDAVAGIGIDPDWFMARIGEQEIKDKLRDETS
                     KAAARGVFGAPTIFVADKMFWGNDRLDYVDDYLKGGL"
     gene            complement(2379..2972)
                     /locus_tag="VFJ67_05660"
     CDS             complement(2379..2972)
                     /locus_tag="VFJ67_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HET7289908.1"
                     /translation="MGKKVEFYYDFSSPYTYIASTRIEKICEENGAELEWKPILLGGL
                     FNDTGVKPAKEIENKFAYVKQDTQDSANRYGVEFHFPAVFPLNSVKTMRGAFAAAEKG
                     KLTEYNHEMFRLYWTEGRDLSKDNELRDAVAGIGIDPDWFMARIGEQEIKDKLRDETS
                     KAAARGAFGAPTIFIGDKMFWGNDRLDFVDRHLKGTL"
     gene            3135..>4900
                     /gene="hemE"
                     /locus_tag="VFJ67_05665"
     CDS             3135..>4900
                     /gene="hemE"
                     /locus_tag="VFJ67_05665"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET7289909.1"
                     /translation="MTSNFSFNGLRVTAFESRRASEIEKLILYHGGVPRVAPSMREVP
                     LSESGEAVKFAEKLARGKFDIVIFMTGVGTRALAQAVSGEVPLDDFTKALAKTKIVAR
                     GPKPVAALRELGLKPDITVPEPNTWRDVVSTLDAELAISGKNVAVQEYGISNEEFINA
                     LKKRGAYVTPVPVYKWDLPEDTAPLRKAIKSITEKKEDISLFTSSQQIYHLFKIASEE
                     GLADKLKEGFKDVAIGSIGPTTTEALTRFGLAADYEPDSPKMGNLIREMARCGGELLR
                     KKRTAHGNGVDTNSWHRVQMVWNGGREDTRKQITDNSAFMKACRREKTDFTPVWLMRQ
                     AGRFMREYRELRARVSFLDLCKTPELAAEVTLSAVDRLGVDAAIIFSDILLIIEPLGV
                     DLEFSKGEGPRILRPVRSGNAVDRLREFEPESMGFVYEAVRITRRALDPEKALIGFAG
                     APFTVASYVIEGGGSRNYENTKQLMYNDREAWHTLMARLTDATTAYLNKQIESGADAV
                     QLFDSWVGCLSPDDFAEYVQPHMKKLIGAVTKGVPVIYFGTGTSALLELMRECGSSMI
                     GFDWRVDLASAWERVGYDVAVQG"
BASE COUNT         1131 a         1370 c         1322 g         1077 t
ORIGIN      
        1 atgtggatat tgaatcgaag cgttttttct tcgacgtgaa agaaaaccac aaaggcaaat
       61 atctcagaat aacggagctc agcggcggca ggtcctgcat agtaatacca ctcggcggga
      121 tcaacctttt caaggagaga cttggagagg tgatacagga agccgagaag ctcatcgaca
      181 ctaacggcag catgtagttc cccggggtcg gttattcccg caaggaaata ctcccgtatc
      241 tatcaacagc tgaaaaacga cggagccggc cttttgagcg gacggggtgt tgcattattg
      301 tcagtatgac aatccaggcg aacctcgcct aatccggacg tatcagggtg cggtattgcc
      361 gtctagccgc cgaaatcggg gacagattcc ccgctcagca tctcgtcgta ggtctccctc
      421 ttcctgacca cgcggaactc cccgcctttg actagtactt cgggcacacg cggtcgcgta
      481 ttgtagttgg aagacatggc gaacccgtat gcgcccgcgc tcatcacagc gagcaggtcg
      541 ccgctcgaaa cagccggaaa cttcctgtct ttggccagaa aatccccgga ctcgcatatc
      601 gggccgacta tgtccacctt atctttcgct ttagcgggcc cgctgacggt cagtatctca
      661 tggtaggatt cgtagaacgc cgggcgtatc aggtcgttca tcgccgcgtc gactataacg
      721 aacttcttag ccgtcccttc cttgagatag aggaccttcg taacgagtac gcctgcgttc
      781 ccgacgagcg acctcccggg ctcgagcacg agcttgtagg gcttgcagcc gaaagcttcc
      841 ttgatcgcct gtgcgtactc cttcggatgg ggcggtatct cgtccatctt atagctgata
      901 ccgaggcctc ccccgatgtc tatgtattcg atccctatgc cgttcgagag cagatcgtcc
      961 gcgagcttta cgagcttctt cagcgaatcg acgaacgggg tgagctcgaa tatctgggag
     1021 ccgatgtgcg cgtcgatcgc cgtgacgcga atacccttca tcccggccgc ctccttatag
     1081 acctcgaacg ccctgctgat ctctattccg aacttgctct tcttgaaccc ggtcgagata
     1141 tatggatggg tcttcgggtc tatgtccggg ttcacacgga gcgcaacagg ggcacgcgtg
     1201 ccgagcctga ctccgacctc gttcaggagg cgtagctctt cgaccgactc gacattgaaa
     1261 aacaatatcc cggcttcgat cgccgctttc atctcctctt cggtcttgcc cacgcccgaa
     1321 aatacaacct tcgacgggtc gccgcctgcg cgtctgaccc ttatgagctc gcctaaggaa
     1381 acgatatcga agcccgctcc aagccgcgca aaagaattca ggatcgcgag attggaattc
     1441 gccttgaccg aatagcacat gagcgtgtcg atgccgaaaa acgcttcttt ataagtgagg
     1501 aacgcgtcct cgatcacggc gcagctataa atgtaacacg gggtgccgac ggattcagcg
     1561 attttcctgg cggggacttc ctctataaaa aactccccgt ccctgtattg ataagaatcg
     1621 tgcatgacct gaattctcct tggaacgagc cgggaagaaa tttacacaaa ttatttcaaa
     1681 tgaaaacagg gactaataaa gcatgagact atcaagaaca tgtaccggac atccgaggct
     1741 acaatccccc ctttaaataa tcgtccacat aatcgagcct gtcgttcccc cagaacatct
     1801 tgtctgcgac gaaaatggtg ggagccccga acacaccgcg ggctgccgct ttggacgtct
     1861 catccctgag tttgtccttt atctcctgct cgccgatacg cgccataaac cagtcggggt
     1921 cgatgccgat ccccgcgaca gcatcccgga gctcgtcgtc cttgctcagg tccctgccct
     1981 cggtccagta gagcctgaac atttcatgtg tatactcagt cagcttaccc ttttcggccg
     2041 cggcgaaagc ccccctcatc gcttttacgc tgtgaagagg aaataccgac gggaatttaa
     2101 aatcgactcc gtagtgcctc gccaaatcct gcgtgtcgtg ttttacatag gcgaatttgt
     2161 tggctatatc caccggtgct ttcacacccg tttcgatgaa gaggccgccc ataaaaaagg
     2221 gcttccattc gagctccgcc ccgttgtcct tgcatatctt cccgattcta ctagaagcaa
     2281 tatatgtata agggcttgtg aaatcgtaat aaaattccac tttctttccc attcctccgc
     2341 tccgggatgc gttgttggta aattattata gaaacgctct aaagagtccc ttttaaatgc
     2401 ctgtccacga aatctagcct gtcgttcccc cagaacatct tgtccccgat aaatatggtt
     2461 ggggccccga acgcccccct cgcagcagcc ttagaagtct cgtccctgag tttgtccttt
     2521 atctcctgct cgccgatacg cgccataaac cagtcggggt cgatgccgat ccccgcgaca
     2581 gcatcccgga gctcgttgtc cttgctcagg tccctgccct cggtccagta gagcctgaac
     2641 atctcgtgat tatattcggt cagcttaccc ttttcggcgg cggcgaaagc ccccctcatc
     2701 gtctttacgc tgttaagagg gaaaacggcg gggaaatgaa actcgacgcc gtaacgattc
     2761 gcagaatcct gagtgtcctg ctttacgtaa gcgaacttgt tctctatctc tttcgcgggc
     2821 ttcacaccag tgtcgttaaa aagccctccc agcagaatgg gcttccattc gagctcggcg
     2881 ccgttctcct cgcatatctt ctctatacgc gtcgaggcta tgtatgtgta agggctgctg
     2941 aaatcgtaat aaaattcgac cttctttccc attcttagtc tccaagtcgc attcgtgtaa
     3001 aggggattat atgaaattag gtttacaata tcaagcggga gatccggcgg atgacgtaac
     3061 cttacgggcc ccggacgcga ttgaccgcag tcttacctgt gataaactca gatatataat
     3121 tataaccagc caagatgacg agtaattttt cattcaacgg actcagggtc acggcgtttg
     3181 agagccggag ggcgagcgag atagagaagc tgatactcta tcacggaggt gtgccgaggg
     3241 tagcgccttc catgcgtgaa gtcccgctct cggaatccgg tgaggctgtc aaattcgcgg
     3301 aaaagctcgc ccgcggcaaa ttcgacatcg tgatattcat gacgggagtc ggcacgcgcg
     3361 ctcttgcgca ggccgtttcc ggagaggttc ctctcgatga tttcacgaaa gccctggcca
     3421 aaacgaaaat agtcgcgcgc ggtccgaaac ctgtagccgc gctcagggag ctcgggctca
     3481 aacccgacat aactgtccct gaacccaaca cgtggcgcga cgtggtatcg acgctcgacg
     3541 ccgagctcgc gataagcggc aaaaacgtgg ccgtacagga gtacggtatc tcgaacgagg
     3601 agttcataaa tgctctcaag aagaggggcg cttatgtgac cccggtcccc gtatacaagt
     3661 gggacctgcc agaggatacg gcgccgctca ggaaagccat aaaatcgatt acagagaaaa
     3721 aagaggatat atctctcttc acgagctcgc agcagattta ccaccttttc aagatagctt
     3781 cagaggaggg tcttgccgat aagctgaaag agggatttaa ggacgttgcg ataggttcca
     3841 tcggacccac gaccacagag gcgctcacga ggttcggact cgccgccgat tacgagcccg
     3901 acagcccgaa gatggggaac ctcatacgcg agatggccag gtgcgggggc gagctgctca
     3961 ggaaaaagag gacggcccac ggtaacggcg tggatacgaa cagctggcac cgcgttcaga
     4021 tggtctggaa cggcggcagg gaagatacga gaaaacaaat taccgataat tccgcgttca
     4081 tgaaggcgtg caggagggaa aagaccgatt tcactccggt atggctcatg cggcaggcgg
     4141 ggagattcat gcgcgaatac agggagctcc gggcgagggt gtctttcctc gacctgtgca
     4201 agactcccga gctcgcggcc gaggtcacac ttagcgcggt tgacaggctc ggcgtggacg
     4261 cagcgataat tttctccgac atattgctga taatagaacc actcggggtc gatctcgaat
     4321 tctcgaaggg cgaaggaccg agaatcctga ggccggtccg ctccggaaac gccgtcgacc
     4381 ggctgagaga gttcgagccc gagtcgatgg gcttcgtcta tgaggcggtc cgaatcacgc
     4441 gccgggcgct cgacccggag aaggcgctca tcgggttcgc gggtgcgccc ttcacagtgg
     4501 cctcatacgt catagaggga ggaggctccc ggaattacga gaacacgaag cagctcatgt
     4561 ataacgacag ggaggcctgg catacgctca tggcaaggtt gacggacgcc accaccgcgt
     4621 acctgaacaa gcagatagag tcgggcgcgg acgcggtgca gctcttcgac agctgggtcg
     4681 ggtgtctctc tcccgacgat ttcgcagaat acgtacagcc gcacatgaag aagctcatag
     4741 gcgcagtcac gaaaggcgtg cccgtgatat atttcggaac ggggacgtcc gcgctcctcg
     4801 agctgatgag ggaatgcgga agctccatga taggttttga ctggagggtg gacctcgctt
     4861 cggcttggga gagggtcgga tacgacgtcg ccgtgcaggg
//
LOCUS       DASSJH010000264         4297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_64206, whole genome shotgun sequence.
ACCESSION   DASSJH010000264 DASSJH010000000
VERSION     DASSJH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64206"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFJ67_05670"
     CDS             complement(<1..385)
                     /locus_tag="VFJ67_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET7289910.1"
                     /translation="MLKTPEEYVQSLRELDPVVYMKGKRVESVPDEPLLEPGVNAVAV
                     TYEFALLPEYTDTMTAVSHVTGRKVNRLLHINTSQNDLLKKLEMVRLLCRETGCAQRY
                     LCHDALNALYETTHNLDLHFGTDYHV"
     gene            493..633
                     /locus_tag="VFJ67_05675"
     CDS             493..633
                     /locus_tag="VFJ67_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289911.1"
                     /translation="MNVKLRESMDDGKIKKGEKQFIYPSFPFLKRVQDMDEKKQKSTD
                     RT"
     gene            complement(903..1637)
                     /locus_tag="VFJ67_05680"
     CDS             complement(903..1637)
                     /locus_tag="VFJ67_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7289912.1"
                     /translation="METHILIIAFASFFGAAFLKGLTGLGFMSICLPLISSFTDITVA
                     IPLVIIPSLASNIMVIAQARRTSESLKRFWPLYLSALPGLYAGVSVLHQSGNSVAKAV
                     LGAMSIAYSLYLLLNVNIVVGNKYEKLLNVPVGLVNGFLNGLTGTQVIPMLPYLISLE
                     LHRDLLIGAINIGFTFSTLVLLALLGKFDFLSLHIIGISLAGIIPVSIGIYLGGKLRY
                     HISESRFRIAVLVILLIIGINLILNR"
     gene            1983..3131
                     /gene="chrA"
                     /locus_tag="VFJ67_05685"
     CDS             1983..3131
                     /gene="chrA"
                     /locus_tag="VFJ67_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008183941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HET7289913.1"
                     /translation="MSDEDKQEYGRVKELAWLFLRLGATAFGGPAAQIAIMHDETVRR
                     KKWLDDKAFLDLIGAVNLIPGPNSTEVAIHVGYLRAGWRGLVTAGACFIIPAAVIVTA
                     LTWVYVRYGSTPEGNHFFYGIKPVIIAVILHALFFIGRKAVTEPVAILTGTAVAAAYF
                     FGVNEIALLFLGGLFVMLWKNVGSLRGGSAAAFLIPFLGLDGAAAEAKGASLTSIFLV
                     FLKIGSVLYGSGYVLLAFLNSDFVEHRGWLTHQQLLDVVAIGQVTPGPWFTTATAIGY
                     LLGGVPGAALATLGIFLPSFIFVALSNPLIPRIRKSPWAGAALDGVNAASLGLMAGVM
                     VDLAKASFPDPLTILIGLAAAGALFLLRMNPTWLMLAGGAVGLLTGLL"
     gene            3665..4111
                     /locus_tag="VFJ67_05690"
     CDS             3665..4111
                     /locus_tag="VFJ67_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289914.1"
                     /translation="MPDQKGRKDLKANRDLPVMKGKQVSSLHLRKRRAQTARTAGRKS
                     KSVSTPITTACWTRQKFQTHITHATASRVCKALRETRDRRVQQGLKDRREFKVTPDQQ
                     GHRVIRVRREYRVMPGNRDHKVCKVMWDQQGHKVRRVIRARTDSQV"
     gene            complement(4108..>4297)
                     /locus_tag="VFJ67_05695"
     CDS             complement(4108..>4297)
                     /locus_tag="VFJ67_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289915.1"
                     /translation="CVSLQALNTLRTLRPLLPVANEFGAVYLSGIQRTAIVIVYSGIY
                     LYSTNYAVRSGSFDCLNN"
BASE COUNT          953 a         1067 c         1358 g          919 t
ORIGIN      
        1 tgacgtgata gtcggtgccg aagtggaggt cgagattgtg ggtcgtttcg taaagcgcgt
       61 tgagcgcgtc gtggcagagg tagcgctgcg cgcagccggt ctcgcggcaa aggagcctca
      121 ccatctcgag cttcttgaga aggtcgttct gtgaggtgtt gatgtgaagg agcctgttca
      181 ccttcctgcc ggtgacgtga gagacggcag tcatggtgtc ggtgtattcg ggaaggagcg
      241 cgaactcgta ggtgaccgcg acggcgttca cgcccggctc gagcagaggc tcgtcgggga
      301 cgctttcgac gcgcttgccc ttcatataga cgaccgggtc gagctctctc aggctctgga
      361 cgtattcttc gggggttttg agcacgggtg ttttctccct tgtccgccgg gggcgggaac
      421 gcggcccgtc cctatttgaa gatagtctca taaaggggag gctgtgtcaa tcggggagag
      481 ggcgggtttt ccatgaatgt aaagctaagg gagagtatgg atgatgggaa aataaagaaa
      541 ggagaaaagc aatttattta tccttctttt cctttcctga aacgagttca ggacatggat
      601 gaaaagaagc aaaaatcaac cgacagaaca taatttgcta aaaatatcgc attaaggcta
      661 aaatcgttca attccacccc aattagcaaa gtagttaagt gatagccgaa gttaattagt
      721 ctcatactgg taggggaggg cgtccgaatg ctcgcttgct tgcattcgtt ccctgaacga
      781 ttttcacgcc ttaatactct attttcttaa cgcaaattct gtaatgtcgg aaatactccg
      841 tccttcgatc cttcgacttc gctcaggacg aacggctttt gttggataat cgaatgtcat
      901 cactagcggt taaggatcag gttgattccg attatgagga ggatgacgag taccgcgatg
      961 cggaacctcg attccgagat gtgatatcgc aatttcccgc cgagatagat gccgattgag
     1021 acggggataa tccccgcgag cgaaattccg attatatgaa gcgacaggaa gtcgaacttg
     1081 cccagcagcg cgaggagcac gagcgtcgag aacgtgaagc cgatgtttat cgcgcctatg
     1141 agaaggtccc tgtgcagctc taaagatatg agatagggga gcatgggtat gacctgagtg
     1201 cccgtgagtc cgttaaggaa cccgttcacg agcccgaccg ggacgttcag tagtttttcg
     1261 tacttattcc ccacgacgat gttgacgttc aaaagcagat agagcgagta cgcgatcgac
     1321 attgcgccga gcacggcctt cgcgaccgag ttgcccgact gatggagcac gctcacgccc
     1381 gcatagaggc cggggagcgc cgagaggtaa aggggccaga acctctttag gctctcgctc
     1441 gtcctcctcg cctgcgcgat gaccataatg ttgctcgcga gcgacggtat gatgacgagc
     1501 ggtatcgcga ccgtgatgtc ggtgaagctc gatatgagcg ggaggcatat gctcatgaag
     1561 ccgagcccgg tgaggccttt cagaaaagcg gcaccgaaga atgaggcgaa ggcgatgatg
     1621 aggatgtgtg tttccatgcg ggggccggtc ccggcttcgg gcttctcaga atataatgag
     1681 gaggggaggg gaaggcaagc gggttttatt atcgcggctg gaagccgctc ctacaatttg
     1741 ttaaaggcga gatacccgat tctttttgga gggcacccga ttgtacgcac gcttacaatc
     1801 gttgtttggg aatgacaaga atttaatgta cacccccacc cataccctcc ccccttaagg
     1861 gggagggaaa taaaagatat gcgatattaa atcctgacgt catcttcggg gtccggtgaa
     1921 tccactttat atagtcttca tatataatga catctgactc ccactaactt tcggatggaa
     1981 aaatgagcga cgaggacaaa caggaatatg gcagggtgaa ggagctggcg tggctgttcc
     2041 tgaggctggg ggcgaccgcg ttcggcgggc ctgccgcgca gatcgccata atgcacgacg
     2101 agaccgtgcg caggaagaag tggctcgacg ataaggcctt cctcgactta atcggcgccg
     2161 tgaacctgat accggggccg aactcgaccg aggtggctat acacgtgggt tacctccgcg
     2221 ccgggtggag ggggctggtg acggcggggg cgtgcttcat aatccctgcc gcagtcatcg
     2281 ttaccgcgct cacgtgggtt tacgtcaggt acggctcgac tcccgagggg aaccattttt
     2341 tctacggcat taagcctgtg ataatcgcgg tgattctcca tgcgctgttc ttcatcgggc
     2401 ggaaagctgt gacggagccc gtggcgatat tgacggggac agccgtagca gccgcatatt
     2461 tcttcggagt gaacgagatc gcgcttcttt tcctgggcgg gttattcgtg atgctctgga
     2521 agaacgtagg cagtctgaga ggcgggagcg ccgccgcgtt tctcattccg ttcctgggat
     2581 tggacggagc cgcggccgag gcgaaggggg cgtcgctcac gtcgatattc ctcgtattcc
     2641 tcaagatagg gtccgtcctc tacgggagcg ggtacgtcct gctcgcgttc ctgaattcgg
     2701 actttgtcga gcacagggga tggctcacgc accagcagct gctcgacgtc gtggcgattg
     2761 gacaggtgac gccggggccg tggttcacga cggcgaccgc gataggctat ctgctcgggg
     2821 gagtgccggg cgcggccctc gcgactctcg gcatatttct gccttctttt atattcgtcg
     2881 cgctgagcaa ccctctcatt ccgaggatca ggaaatcgcc gtgggcgggg gccgcgctcg
     2941 acggcgttaa cgcggcgtcg ctcgggctga tggccggcgt gatggtagat ctcgcgaagg
     3001 cttctttccc cgacccgctg actatactga taggcttggc cgcggccggg gcgctgttcc
     3061 tgctccgcat gaatccgacg tggctcatgc tcgccggagg ggccgtgggg ctgctgacgg
     3121 ggctcttgtg agggggcttt aagggactgt tcggcggatt taaactttct ctatcgaaac
     3181 aagcggatta tcgtaaatag ttcaccatta cggttaccta ttttcaaaaa tagccatata
     3241 atttggtcat aggcaaaggt ggctccactt cggaaactcc ttacaactgt tcttttgcgg
     3301 gatccgtgca ttgctgactt tgtaactgga gccataaatc ggcgtgtttt ttatcggtta
     3361 cacaaaatgc ataaagaagc gtatgaagta tctcgcgcaa tgagaatctc gcggcccggg
     3421 caaatcaggg tcacgaaatc attaatattt ctgctcatat cggctttact gcttgtagta
     3481 ctggcttgcg acagcgggag cggcagcgtc ggcgacgggg gcggggacgg atcgggtctg
     3541 gaaggcccac ccggaccgcc gggacccccg ggacctcccg gacctcaagg accgcccggg
     3601 cctccgggtt ccggagacgg ttcaggactg ccgggacctc aggggccgcc cggacctcaa
     3661 ggggatgccg gaccagaagg gccggaagga cctcaaggcg aaccgggacc tgccggtgat
     3721 gaagggcaaa caagtctcat cgttacatct acggaagcgc cgggcgcaaa ctgcgcgaac
     3781 ggcgggacga aagtcgaagt cggtctcgac tccgataaca acggcgtgct ggacgcggca
     3841 gaaatttcag acacatatta cgcatgcaac ggcgagcaga gtctgcaagg cgctcaggga
     3901 gacgcgggac cgcagggtcc agcagggcct caaggaccgc agggaattca aggtgacgcc
     3961 ggaccagcag ggccacaggg tgatcagggt ccgcagggag tacagggtga tgccgggcaa
     4021 cagggaccac aaggtttgca aggtgatgtg ggaccagcag ggccacaagg tgcgcagggt
     4081 gatccgggcg cggacggatt cacaagttta attgttcaga caatcgaagc tcccggagcg
     4141 aactgcatag ttggtggagt aaaggtagat gccggaatag acgatgacaa tagcggtacg
     4201 ctggatgccg gagagataga cagcaccgaa ttcgtttgcg acagggagga ggggcctcag
     4261 ggtgcgcagg gtattcaggg cctgcaagga gacacag
//
LOCUS       DASSJH010000265         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_822325, whole genome shotgun sequence.
ACCESSION   DASSJH010000265 DASSJH010000000
VERSION     DASSJH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822325"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFJ67_05700"
     CDS             <1..352
                     /locus_tag="VFJ67_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7289916.1"
                     /translation="DTKEVKKIVSRIKEGPSVLIAPEGTRGKEGKLLPFQKGGFLIAM
                     LAGVPVVPMVITGGLDIMPKDSKVLRPGTMKIRILPPINVADLPPGREGREELMRIVR
                     SQMEEVLDREERRE"
     gene            612..2300
                     /locus_tag="VFJ67_05705"
     CDS             612..2300
                     /locus_tag="VFJ67_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289917.1"
                     /translation="MNSHKLADLELSTIITLAGMPPQKELVNPRMPSEMAKKVRVTFR
                     PLPKDFGNQVIMRFRDKLEQELKENGVQLIPWDEAAEVPPGIVSKILRTRKVKSSIHA
                     VVDVKREYSLARSILSALAEKIYIHTRKPERSVMEILKISGWADDFTARYVQDPFNTQ
                     IITLMPLEPEFADGSTTYDRKIALGLKNLITTMSEIVMGIAPDRFSLVNMNLSDSIYR
                     NEELDEFVLNSLIPKIYAPIKPPVLNRFKKGEFEPSHSVFAKQLADLGRLVEPTSLFP
                     QGSKFSEKITRLSHRDVVEKILEGRTGVSYGFIAIAEAPVYEGPVTVTKEEWESFPVV
                     ESIKDERVRENAQGRWYVKTIIRGQEIYQQIPDIWIVTSRSGSNKTNLDPNTDVVRIG
                     LIKGKLNLETPRGVDLHRRDIRPSFDTYVILAQAMAAALYTPELIKNGLPILHFHGYP
                     DPTFFGQSEYFAGARNPSLPCGTVEAALLNYSAIYEVADRNGDSIKMLCLVEADHGVN
                     ILGFDRDYLVKRLSEGVDQGHISLGGKYLPDLRKSDLRADGKEQDLEKAAGSAH"
BASE COUNT          601 a          605 c          665 g          468 t
ORIGIN      
        1 agacacgaag gaagtcaaga agatagtgag caggataaaa gaagggcctt ccgtcctcat
       61 cgcccccgag ggcacaaggg gcaaggaagg caagctcctc ccctttcaga agggggggtt
      121 cctgatagcg atgctggcgg gtgttccggt agtgccgatg gttattacgg gcggtctcga
      181 tatcatgccc aaggactcga aggtgctgag gccgggcacg atgaagataa ggattctccc
      241 tcccataaac gttgcggatc tccctcccgg gagagaggga agagaggagc tcatgcgcat
      301 cgttagatcg caaatggagg aagtgctcga ccgcgaagag aggcgcgagt gaaaatagtt
      361 ctatcattcc cggggcatgt aaagattgtc atatgcgtat tctcgtctat aataaattct
      421 gtccgtgccc tttattgagt aacaacggcc gatagtctat aatttatgag ctatcggcgg
      481 ggttcatcga aacattaata taaaaacaca atcgctcaat ttgaatccgc ggctttctga
      541 tcatgatata ttgatattcg cacattttga gggacgcctc ttgaacagga ttgtcgtgag
      601 ggggagagaa aatgaatagt cataaactag ctgacctcga gctcagcacg ataataacgc
      661 ttgcaggaat gcccccgcag aaagagctcg tcaaccccag gatgccttcc gagatggcga
      721 agaaggtgag ggtgacgttc aggcccctgc cgaaagattt cgggaaccag gtgatcatgc
      781 gcttcaggga taagctcgaa caggaactaa aggaaaacgg cgtccagctg atcccctggg
      841 acgaagccgc tgaagttccg ccggggatcg tttcgaagat attacggacc aggaaggtca
      901 agagcagcat ccacgctgtc gtggacgtga aaagggagta ttccctggca cgcagcatcc
      961 tgagcgcgct cgccgagaag atttatatcc acaccaggaa gcccgagaga tcggtaatgg
     1021 agattctgaa aataagcgga tgggctgatg attttacggc aaggtacgtt caggacccgt
     1081 tcaacacgca gataatcacg ctcatgccgc tcgagcccga gttcgcggac ggcagcacca
     1141 cctacgacag gaagatagcg ctcgggctca agaacctcat caccacgatg tcggagatag
     1201 tcatgggtat agcgcccgac aggttctcgc tcgtcaacat gaacctttcg gattccatat
     1261 accggaacga ggagctcgac gagttcgttc tcaactcgct cataccgaag atttacgcgc
     1321 ccatcaagcc ccccgtgctc aacaggttta aaaaggggga gttcgagcct tcacattccg
     1381 tcttcgctaa acagctcgcc gacctcggca ggctcgtcga gccgacgtct ctgttcccgc
     1441 agggctcgaa gttcagcgaa aagatcacga ggctctccca cagggacgtc gtcgagaaga
     1501 tactcgaagg caggacgggc gtctcttacg ggtttattgc tatcgcagag gcgcctgttt
     1561 acgaaggccc tgttacggta acgaaagagg agtgggaaag cttcccggtc gtcgaaagca
     1621 tcaaggacga gagggttaga gagaacgcac agggcagatg gtacgtcaag accataatca
     1681 gggggcagga gatttatcag cagatcccgg atatatggat agtcacctcc cgctcgggga
     1741 gcaataagac gaacctcgat cccaacacgg acgtcgtgag gatcgggctc ataaaaggaa
     1801 agctcaatct tgagacccct aggggagtag acctccacag gcgtgacatc cggccgtcgt
     1861 tcgacacgta cgtgatcctc gctcaggcca tggcagccgc gctctacacg cccgagctta
     1921 taaaaaacgg cctccctatc ctccattttc acggataccc ggaccccacg tttttcggcc
     1981 agagcgaata tttcgcgggc gcgaggaacc cctctcttcc gtgcggcacg gtcgaggcgg
     2041 cgctgcttaa ctattccgcc atctacgaag tcgccgaccg gaacggggac agcatcaaaa
     2101 tgctctgcct cgtggaagcc gaccacgggg tcaatattct cgggttcgac agggactatc
     2161 ttgtaaagag gctcagcgag ggtgtggacc aggggcacat atcgctcggg ggcaagtacc
     2221 tgcccgatct gaggaaaagc gacctcaggg cggacgggaa agagcaggac ctggagaaag
     2281 ccgcgggttc cgctcattaa ttccgatatt gccaatttct ctcactttgg tatattgtt
//
LOCUS       DASSJH010000266         4996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1249852, whole genome shotgun sequence.
ACCESSION   DASSJH010000266 DASSJH010000000
VERSION     DASSJH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249852"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VFJ67_05710"
     CDS             <1..1263
                     /locus_tag="VFJ67_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289918.1"
                     /translation="AGLALRIFLSQFLTYRSDFSAWTGWGRQLATAGFGEFYGRYWCD
                     YMPGYLYVLWFLDNVNRAMPAIPVDILFKLPANLADLGISILIFFTLRLITSTKNAMI
                     ASVVYFFNPASLSNSTFWGQVDSFHALPILLSVYLGLRRKFILSGMFAALAFMIKPQS
                     LVIFPLIGFLGLKPIFESGYKLTVRSLLPSIELVAAALVTAVIVTLPFIWDGIDSAAY
                     LITGPFDLIRERFGAAYGQYTASSLNAFNFWGAVEMWMSDDTKFLGITYRTIGTLIFG
                     AVYALVVGLLIRFTAALRTKDLSGYGYCVFEAITLVLFTLFLFVTRAHERHLLPTIVF
                     FTLITYRTWVFWYIYAVVSCVYVANMVYSYIQLTTDYKGIPDSLSGYFIPGIFSLYLI
                     GYIIVLLNFVVSTAKHEKSLDTPAPRTP"
     gene            1274..2584
                     /locus_tag="VFJ67_05715"
     CDS             1274..2584
                     /locus_tag="VFJ67_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET7289919.1"
                     /translation="MPEQSFLSKYKAELLLALILVFCFGIRFYRLEIPDSYYFDEVYF
                     AFTAQEMAKGNRAAWESYAKAPEGMAYEWTHPPLGKEISALGILIFGNNTLGWRFFQA
                     LFGSLGALFIYLLGKDLFVAKSAGIIASLLYSFESFVFVLSRITMVDIFISDFLILAS
                     LFIVKYARTRRFANLVLAGFFCGAGMSVKWSGLYITEFLGGVALFLMYYFEVYSTSAR
                     DREFIASVLRIIPRMAFAFMVVPILVYIATYIPFFYNGDTFRDFLTLQANMYGYHGGV
                     TATHPYQSSWWQWPLMLKPVYLSAENLGKGLNAHIYLVGNPFLWYTGLGFLLLGIFQA
                     VRKETPALLFTVLSVFAYWLPWALSPRKVVFLYHFLPSLVFLLMTSVYFLNELWNGSR
                     KGKIFVVLYICIAGGVFFYFYPILAALPIKDTEIDRFMWLRSWR"
     gene            2662..3279
                     /locus_tag="VFJ67_05720"
     CDS             2662..3279
                     /locus_tag="VFJ67_05720"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET7289920.1"
                     /translation="MKRSDISVSIILGIIIAAFVIAILSTLKDELHIRYIPYALLWPV
                     SLVIIPALVILWVYIASHFGSRWHILFQFGKFIPIGVSNTAIDFGILNFLMFMTGIHK
                     GYMFSVFKSISFLFAVANSYVWNKFWTFECRERDGMGKQFIKFIIVSAIGFALNVLVA
                     SFMVNVIGARGGISPVLWANIATLASIILVIIWNFLGYKYLVFNK"
     gene            complement(3281..4498)
                     /locus_tag="VFJ67_05725"
     CDS             complement(3281..4498)
                     /locus_tag="VFJ67_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289921.1"
                     /translation="MEQDNTGRDSPKTVLLIFIIIFALSFVVYLLTTEDHPTPFNNFV
                     RLADAMLHGRLYLTRDVPWLEIIPFQGRYYIVPPPLPAILILPVVAVFGLSTDQTVIS
                     IFFGSLNVSLAYLTARAVSKSRSVQLWTAAMFGFGTIHWWVAAAGGVWTISHTISVTI
                     LFIAILLTLYKRSPFYAGAALGASYWTRLPTVLSLPFFVAMYSDEWYRGGEDLNILKR
                     INLRPLLWLGAGVGIFVVLNACYNVLRFGTPFDISYYLIPGILDEPWYREGIFDISYI
                     PRHLKVIFGGYPRFIDEFPYVTPSWNGMAIWITTPAFIYAFFAGIRSRLAAGCWLSIL
                     LVGLVNFCHGTWGFSQFGYRFAVDFYPFLFLLTVKGIGDEIRWHHKVLIAAGILVNLW
                     GVLWINKFGWVGY"
     gene            complement(4512..>4996)
                     /locus_tag="VFJ67_05730"
     CDS             complement(4512..>4996)
                     /locus_tag="VFJ67_05730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7289922.1"
                     /translation="SSAVLDGFSMSKSEIIGVMDADLSHPPEKIPEMVEPLLNGEADI
                     VIGSRYVDRGGTEDWALRRKISSRIATLAVIGLTRVKDPMSGFFFLKREVIESVPLSP
                     KGFKIALEILVRGKYDRVVEVPIIFRDRIYGETKLSGGVILDYLYHVAKLYGFKILKS
                     "
BASE COUNT         1104 a         1325 c         1270 g         1297 t
ORIGIN      
        1 gcgggtctgg ctctcaggat tttcctctcc cagttcctca cgtacaggtc tgatttcagc
       61 gcgtggacag gatggggacg gcagctggcg actgccgggt tcggagaatt ttatggcagg
      121 tactggtgcg attatatgcc cggttacctc tacgtgctgt ggtttctcga taacgtcaac
      181 cgcgccatgc ccgccatacc ggtcgatata ctcttcaagc tcccggccaa cctcgccgac
      241 ctcggaatct ccatactcat attttttaca ctcaggctta taaccagcac aaagaacgcg
      301 atgatagcgt cggtcgtata ttttttcaac cctgcgtcgc tctcgaattc gaccttctgg
      361 ggacaggtcg attcttttca cgccctgccc atattgctct ccgtgtatct cgggttaagg
      421 aggaaattta ttctttccgg gatgttcgct gcgcttgcgt ttatgataaa gccccagagc
      481 cttgtcattt tccccctcat agggtttctc gggctgaagc cgatattcga aagcggatac
      541 aaactcacgg tccggtctct cctgccttcg atagagctcg tcgcggccgc cctggttacg
      601 gcggtcatag tcacgctccc gttcatatgg gacgggatcg attcggctgc ctatttgatc
      661 accgggccct tcgatctcat aagggagagg ttcggcgcgg cgtacgggca atatacggcc
      721 tcttccctga acgcctttaa tttctggggc gctgtggaga tgtggatgtc cgatgacacg
      781 aagttcctgg gtatcactta caggactatc gggacgctga tattcggagc ggtctacgcg
      841 cttgttgtgg gcctcctgat aaggttcaca gccgcgctca ggaccaaaga tttaagcggt
      901 tacggctact gtgtgttcga ggcgattacg ctcgtgctgt ttacactatt tctcttcgtt
      961 acgcgcgccc atgaacggca cctcctgccg acgatcgtgt ttttcacatt aatcacatac
     1021 aggacctggg tgttttggta tatctacgcc gtggtatcgt gtgtttatgt ggcgaacatg
     1081 gtgtattcct atatacagct tactacggac tataaaggaa tacccgacag cttatccggc
     1141 tattttattc cgggcatatt ttctttgtat ctcattggat atattattgt actcttgaat
     1201 tttgtcgtaa gtacggccaa acatgagaaa tcgctcgaca ctccagctcc ccggacccca
     1261 taagagataa cctatgccgg aacaatcctt tctcagcaaa tataaggccg agctccttct
     1321 cgccctgatc ctggtatttt gcttcggcat caggttctac cgcctggaaa tacccgacag
     1381 ttattacttc gacgaggtct atttcgcctt caccgcccag gaaatggcaa aaggtaacag
     1441 ggccgcatgg gaaagctacg ccaaggcccc cgaagggatg gcttacgaat ggactcaccc
     1501 gccgctcggg aaagagataa gcgctctcgg catccttata ttcggtaata acactctcgg
     1561 atggcgtttt tttcaagccc tgttcggctc attaggggct ttattcatct acctcctcgg
     1621 gaaggattta ttcgtcgcca agtcggcggg catcatcgcc tcccttctct acagtttcga
     1681 gtctttcgtc ttcgtgctga gccgtatcac tatggtcgat atcttcatat ccgacttcct
     1741 catactcgcg tcccttttta tcgtcaagta tgcaaggacc aggcgattcg cgaacctcgt
     1801 cctggccggg tttttctgcg gtgctgggat gtcggtaaag tggagcggcc tgtacatcac
     1861 ggaattcctt ggtggtgtgg cgctctttct catgtattat ttcgaggtat attcgacctc
     1921 ggcacgcgac agggaattta tcgcctccgt tctgagaatc attcctagaa tggcgttcgc
     1981 gttcatggtt gttccgatcc tcgtctacat cgcaacttat attccctttt tctataacgg
     2041 agacacgttc cgggacttcc tcaccctcca ggctaacatg tacgggtatc acggaggcgt
     2101 aaccgcgacc cacccgtacc agtcgtcctg gtggcaatgg cctctcatgc tcaagcccgt
     2161 ttacctctct gcggagaacc tcgggaaggg gttgaacgct cacatatatc ttgtcggcaa
     2221 tccgttctta tggtacacgg gtctcgggtt cctgctgctg ggtatattcc aggcggtgag
     2281 aaaagaaact cccgcccttc tcttcaccgt attgagcgta ttcgcatact ggcttccctg
     2341 ggccctgtct ccgaggaagg tggtgttcct gtatcatttt ctcccttcgt tggtattttt
     2401 actcatgacg agcgtatact tcctgaatga gttgtggaat ggttcgagaa aagggaaaat
     2461 attcgtggtc ctttacatct gcatcgcggg cggcgtattc ttctattttt atccaatcct
     2521 cgcggcattg ccgatcaaag atacggagat agaccgtttt atgtggctca ggtcctggag
     2581 gtagtgtaat cggtttccgc cgggcggtcg ggcccgtgag cgcaatatga gaggctgggg
     2641 caagaagttc taatacctga aatgaaaaga tcggatatat ccgtttcaat aatactaggg
     2701 atcataatag cggcgttcgt aatcgccata ttgagcacgc tcaaggacga gctccacatc
     2761 cgttacatac cttacgccct gctatggccc gtgtccctcg taattattcc ggccctcgta
     2821 atactctggg tctatatagc ctcacacttc ggctccaggt ggcatatcct ctttcagttc
     2881 ggcaagttca ttcccatagg cgtctcgaat accgcgatcg atttcgggat actcaacttt
     2941 ctgatgttta tgacgggtat acacaagggc tacatgtttt cggtcttcaa gagcatatcg
     3001 ttccttttcg ccgtggctaa cagctacgtc tggaacaagt tctggacctt cgagtgccgg
     3061 gaaagggacg gcatgggaaa gcagttcata aaattcataa tagtctccgc gatagggttc
     3121 gcgctcaacg tcctggtcgc ttcgttcatg gttaacgtca tcggcgcgcg gggcggtatt
     3181 tcaccggttc tgtgggcgaa tatagccact cttgcgtcca taatactcgt gatcatatgg
     3241 aacttcctcg gttacaaata cctggttttc aacaaataga tcaatagccg acccatccga
     3301 acttgtttat ccagagcacg ccccagaggt ttacgaggat gccggcggcg atgagcacct
     3361 tatggtgcca ccttatctcg tcgcctatac cttttaccgt cagtaggaac aggaacggat
     3421 agaagtccac cgcgaacctg tatccgaact gggaaaatcc ccatgtgccg tggcagaaat
     3481 ttacgagccc cacgagaagg atggacagcc agcagccggc agccagcctg ctccttattc
     3541 ccgcgaaaaa cgcgtatatg aaggcgggag tcgttatcca gatcgccata ccgttccagg
     3601 acggcgtgac gtaaggaaat tcatcgatga accggggata gccgccgaat atgactttca
     3661 ggtgcctcgg aatatacgag atgtcgaata tgccctcccg gtaccatggc tcatccagta
     3721 tccccggtat caggtaatag gatatatcga aaggagtgcc gaatctaagg acgttatagc
     3781 aggcgttaag gactacgaaa atgcctaccc ccgccccgag ccataagagg ggtctcaggt
     3841 ttatcctttt caatatgttc aagtcctcac ctcctctgta ccattcgtcc gagtacatcg
     3901 cgacgaaaaa aggcagagaa aggacggtcg gaaggcgtgt ccagtatgac gcccccaggg
     3961 ctgcccctgc gtaaaacggg ctgcgtttat agagggtgag aaggatagcg ataaaaagaa
     4021 ttgtcacgga aatagtatgc gagatagtcc aaacgccgcc cgcggcggct acccaccagt
     4081 gtatggtgcc aaacccgaac atggcggccg tccaaagctg tacgcttctg cttttcgaga
     4141 cggccctcgc cgtgagatac gcgagagaga cgttcaggct cccgaagaaa atggagatca
     4201 ctgtctggtc cgtcgaaagg ccgaagacag cgacgactgg cagtatcagt atcgccggga
     4261 gcgggggagg cacgatatag tatcttccct ggaaagggat tatctcgagc caggggacat
     4321 cgcgggtgag gtataacctc ccgtggagca tggcgtccgc gagcctgacg aaattattga
     4381 aaggcgtggg gtggtcctcc gtcgtgagca aatatacgac gaatgaaagg gcgaatatga
     4441 taataaatat gagtaagacg gtcttcgggc tgtctctgcc cgtattatcc tgttccattt
     4501 caaaatcggc tttaagactt gaggatcttg aatccgtaga gtttggcgac atggtaaaga
     4561 taatcgagga ttacgccgcc gctcagcttc gtctccccgt atatcctgtc ccggaagatt
     4621 atcgggactt ccacaactct atcgtatttg cccctgacca gaatttcaag tgcgatctta
     4681 aaccccttcg ggctgagcgg gacgctctcg atcacttccc ttttgagaaa gaaaaatccc
     4741 gacatcgggt ccttgacgcg tgtgagccct atgacggcga gagtcgcgat cctcgaggat
     4801 atctttctcc ggagcgccca atcctcggta ccgcctctat cgacgtagcg gctcccgatt
     4861 acgatgtccg cctccccgtt taaaaggggc tccaccattt caggtatctt ttccggagga
     4921 tggctcaggt cggcgtccat gacgccgatt atttcgctct tcgacatgct gaagccgtcc
     4981 agaacagccg aagaga
//
LOCUS       DASSJH010000267         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1576697, whole genome shotgun sequence.
ACCESSION   DASSJH010000267 DASSJH010000000
VERSION     DASSJH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576697"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..386
                     /locus_tag="VFJ67_05735"
     CDS             150..386
                     /locus_tag="VFJ67_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HET7289923.1"
                     /translation="MDPKEIRALIEAGMPGSSVDVEGDGTHFEAVIVSSDFNGKTLLE
                     RHQQVYKVLGEAMKERIHALSLKTYTPEQWEKIK"
     gene            446..766
                     /gene="grxD"
                     /locus_tag="VFJ67_05740"
     CDS             446..766
                     /gene="grxD"
                     /locus_tag="VFJ67_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HET7289924.1"
                     /translation="MEDVIKKIESQVAKNKVILFMKGTKEMPQCGFSAKVVQILNSHG
                     VDYETVDVLADPEIRQGIKEYSQWPTLPQLFVDGKFIGGCDICVEMEESGELGSILKD
                     AVRE"
     gene            complement(768..1457)
                     /locus_tag="VFJ67_05745"
     CDS             complement(768..1457)
                     /locus_tag="VFJ67_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7289925.1"
                     /translation="MKSAKSDHERMLSVLSLKNKAGWAMEQNISDLADEDLVGLFVAS
                     RDEEAFNEIVNRYGDKVYRLAYRITGSQNDAEEVLQEVFLILVEKLSTFRKESRFSTW
                     LYRVASNASFMYIRNSRKSSDKELSLDDYKPYNDYGALEGVEEKDWSGRPDSQLLGRE
                     GTELIEKAIGELPEEYRVVFHMKDIEKLTSKEISKILKLSVPAVKSRVLRARLFLRDK
                     LSDYYSELNNG"
     gene            complement(1532..2158)
                     /locus_tag="VFJ67_05750"
     CDS             complement(1532..2158)
                     /locus_tag="VFJ67_05750"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7289926.1"
                     /translation="MGRTSCAKENLITSAIELIGTRSYNAVGVQELCEHAGVKKGSFY
                     HFFPSKRDLTLEALDQIWNKFKVETLDPIFNSEISPIDKFKTLLARSYEYQSSRKDCT
                     GCMTGCGFGNLALELSTQDEEIRKKIEEIFCEWTQYLERLILQSIEEGVLPSDTDPAA
                     TAQAMVAYIEGVYLLGKTFNDPCMIQRLGEGVLKLCIPKKKTDVCSQM"
BASE COUNT          547 a          622 c          512 g          559 t
ORIGIN      
        1 cgccgagaag gagcaaaata acgactagga cgataagcgc tgtcctcatg ctcatatcgt
       61 gaattatagg tttcaatcac gcccatttca acagcctgcc cggccgcgcg tttcttgaaa
      121 gggtactaaa aacatttata ataaccctga tggatccgaa ggaaataaga gcccttatcg
      181 aggccggaat gccgggctcc tcggtcgacg tggaggggga cggcacgcat ttcgaggcgg
      241 ttatcgtttc gagcgacttt aacggaaaaa cccttctgga gcgccatcag caggtttata
      301 aagtcctcgg cgaagccatg aaggagagga tacacgcgct ctcgctcaag acatatacac
      361 ccgagcagtg ggaaaagatt aaataggggc cgaatcgcgc agacgagcgg gtcccggccg
      421 ttaaaacatc gggaggcagt ataaaatgga agacgtaatc aagaaaatag aatcgcaggt
      481 agcgaaaaac aaagtgattt tattcatgaa aggtactaag gaaatgccgc agtgcgggtt
      541 ctccgcgaag gtcgttcaga tactcaactc tcacggggtc gactatgaga cagtcgacgt
      601 gctcgccgac cccgagatca gacagggcat caaggaatat tcccagtggc ccactctgcc
      661 gcagctcttc gttgacggga agttcatcgg cggatgcgat atatgcgttg agatggaaga
      721 gtcaggcgag ctcggttcca tactcaagga cgcggtaagg gaataaccta gccgttattg
      781 agctccgaat agtagtccga gagcttatcc ctcagaaaaa gcctcgccct caacaccctc
      841 gatttaactg caggcaccga aagcttcagt atcttcgata tctccttgct cgtgagcttc
      901 tcgatatcct tcatgtgaaa aaccaccctg tattcctcgg gcagctcccc tatggccttc
      961 tctatgagct cagtaccctc cctgccgagg agctgcgaat ccggcctccc gctccagtcc
     1021 ttctcttcga ctccttcgag cgcaccgtaa tcgttatagg gtttataatc gtcgaggctg
     1081 agctccttgt cgctgctttt tctcgaattc cttatataca tgaagctcgc gttcgaggcc
     1141 accctgtaaa gccacgtaga gaacctcgac tccttcctga acgtggacaa tttttcaacc
     1201 agtatcagaa agacctcctg caggacctcc tcggcgtcgt tctggctgcc ggtaatcctg
     1261 tatgccagcc tgtaaacctt atccccatac ctgtttacaa tctcgttgaa cgcctcctcg
     1321 tcccgactcg cgacgaacaa acccaccagg tcctcatcgg cgaggtcgct tatgttttgc
     1381 tccattgccc atcccgcttt attcttgaga ctcaacaccg aaagcattct ctcgtgatca
     1441 gatttagcgg acttcataat tactcctcct gattcgataa tatagttgac cggtctgttt
     1501 aaaaataata aaaaaatata tgcccggtct tttacatctg agaacacaca tccgtcttct
     1561 ttttcggtat gcacagcttg agcacacctt caccaagcct ttgtatcatg cacgggtcat
     1621 taaaggtctt gcccagtaag tacacccctt ctatataggc gaccatggct tgagccgtgg
     1681 ccgccgggtc cgtatccgac ggcagcacgc cctcttcaat ggactgaaga attagtcttt
     1741 ccaggtactg ggtccactcg cagaatatct cctcaatctt tttccttatc tcctcgtcct
     1801 gcgtgctcaa ttcgagcgcg aggttcccga acccgcatcc cgtcatacac cccgtgcagt
     1861 cctttctgct cgactgatat tcataagacc tcgctagcag cgtcttgaac ttgtcgatag
     1921 gtgaaatctc ggaattaaag atcgggtcga gggtctccac cttgaactta ttccatatct
     1981 ggtccagggc ctccagggtt agatcgcgct tcgaagggaa gaagtgatag aagcttccct
     2041 tcttcacccc ggcgtgctcg cacagctcct gcacgcctac ggcgttatag ctcctcgtgc
     2101 cgataagctc tatcgcacta gttatcagat tttcctttgc gcagctcgta cgtcccatga
     2161 ctttccttta gttgaccagt tggtaaaata atatcacttc agacgccgtt gtcaagtaaa
     2221 tggatttaaa aacccggatc
//
LOCUS       DASSJH010000268         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_173636, whole genome shotgun sequence.
ACCESSION   DASSJH010000268 DASSJH010000000
VERSION     DASSJH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173636"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFJ67_05755"
     CDS             <1..530
                     /locus_tag="VFJ67_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HET7289927.1"
                     /translation="VIGVTTRWLFLIVFLILSPLVYLISFGIGIEKAKPVEFCNSCHI
                     MNSHVDDMKDPDSESIAALHYKYRWISDNQCYTCHSDYGLYGAVGAKFAGVRHILNYY
                     VVGYPNKLEIRGTYNNERCLFCHAPVESYQDIEEHQEYAAEIKSSKQSCFGADCHVSP
                     HPNETKEIADNWRIK"
     gene            527..739
                     /locus_tag="VFJ67_05760"
     CDS             527..739
                     /locus_tag="VFJ67_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289928.1"
                     /translation="MNSKRNYKILLLTIAGILNILGMLLIIIPVFELTPLTLIVSAVF
                     GGGLVILSIIIYATVVTRDLIKKGIL"
     gene            complement(976..1398)
                     /locus_tag="VFJ67_05765"
     CDS             complement(976..1398)
                     /locus_tag="VFJ67_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7289929.1"
                     /translation="MIENIKIKEIMTTDVMTVHENENLSVVAKIFRESPIHHVPVLKG
                     KRPVGIISTQDLFKLIFDFDSTDTRMLDTLLDHTYKIKDVMSNKLVVFEEESMLKDAA
                     KILSDSSLHSIMIVNSKGDLTGIVTTTDLMRFLYKNIE"
     gene            complement(1676..2374)
                     /locus_tag="VFJ67_05770"
     CDS             complement(1676..2374)
                     /locus_tag="VFJ67_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7289930.1"
                     /translation="MRRRFKSGSTCSLRADTIFSGLDSEGVVELESKMQRFDYPKDVV
                     LFLKGERPRGNYCICSGRVKLSVHSPDGRGLIAGYPSAGSVIGVRAVLSGKSHDFTAR
                     TVEESKLNFVDKEDFLAFLRRDGGVCLRLAESLCEELSRTYADLKNAALAGPEERLAS
                     LLLRLCEEVGEPTPDGFVINMGVCQEEMAEMAGMSRRTLTRAINKLRRMELIKCGRRV
                     TVVRDKTALQNLIT"
     gene            complement(2358..>3405)
                     /locus_tag="VFJ67_05775"
     CDS             complement(2358..>3405)
                     /locus_tag="VFJ67_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HET7289931.1"
                     /translation="CRADARRCRDPRELTTYQGRNLINDVAGFGSPLLVLTGGDPLKR
                     DDIYDLISYSVEKGLRTTVSPSVTLLLTDESVRKLREAGISRLAVSLDGSMPSIHDSF
                     RGVEGSFARTVEILSLCALEGIPIQVNTTVTRYNLGDFENIAKLIGGFGPVLWSVFFL
                     VPTGRGRAADGVSAEEYEEVFGKMYELSKSVSYDIKSTEAPHYRRYAAQARVRESRAG
                     KCPLDAAAERKRDAIGRAPRGINDGKGFVFVSHTGEVYPSGFLPLSAGNVKEGSIVDI
                     YRNSPLFREIRDYDRLKGKCGACEFKNICGGSRARAYALTGDYMKSEPYCVYVPRDYC
                     TKESGEEPARAAKI"
BASE COUNT          798 a          919 c          795 g          893 t
ORIGIN      
        1 gtgtaatagg ggtaacaacc agatggcttt ttttgattgt gtttttaatc ctttccccgc
       61 tggtctattt gattagtttc ggcattggaa ttgagaaagc aaagcccgtt gaattctgta
      121 acagctgcca tataatgaat agtcacgtgg acgacatgaa agatccagat agtgagagca
      181 ttgccgctct tcattataag tatcgctgga tttccgataa tcaatgctac acgtgtcact
      241 cagattacgg gctttatgga gcagttgggg caaaattcgc tggcgtcagg cacatcctga
      301 attactacgt tgttggttat ccgaataaac tagagattag gggcacttat aacaacgaga
      361 gatgcctttt ctgccatgcc cctgtcgaat cctatcagga catagaagaa catcaggagt
      421 atgcagcaga aatcaaatcg agcaaacaat cttgttttgg cgcagactgt cacgtatctc
      481 ctcaccccaa tgaaacaaag gaaatagcgg ataattggag gatcaaatga attcaaagag
      541 aaattataaa atattgcttc ttacgattgc cgggatatta aacatcttgg gaatgctttt
      601 aattattatt cctgtatttg agctaactcc cctaacactt atcgtctctg cagtttttgg
      661 tggaggctta gtaatacttt caatcattat atacgccaca gttgtaacaa gggatctaat
      721 aaaaaagggc atcctctagc aagatgctcc ggtcgtatgc ggattcttct ggactcatca
      781 gtcgctgata ttttgatcca ttcgacaggc tcataacgaa cgtgtgatta ttcggccccc
      841 gcaaactgag gataaaattg gggtgaacca cctcggaatt gtaaaagtac ccctaatgct
      901 gaattcttta tagcataatt gggtaggata gcacacgagc gcaggttctg cttcacaaat
      961 tgtgggttgg tctcgctatt cgatattctt atacaggaat ctcatcaggt cggtcgttgt
     1021 cacaatgccg gtgaggtctc ctttcgaatt gactatcata atcgaatgga gcgagctgtc
     1081 tgagagtatc ttcgccgcat ccttcagcat ggattcctcc tcgaatacga cgagcttgtt
     1141 cgacatgaca tccttgatct tgtaagtgtg atcgagaagc gtgtcgagca tcctagtatc
     1201 ggtcgaatca aaatcgaata tgagcttgaa cagatcctga gtcgagatta tacccacggg
     1261 cctcttcccc ttgagcacgg ggacgtggtg gatggggctc tccctgaata tcttcgcaac
     1321 cacactcaag ttctcgtttt cgtgcacggt catcacgtcc gttgtcatga tctcttttat
     1381 cttgatgttc tcaatcatat tcagtccctc acgtcagtta tttttagcta tggattatac
     1441 atacccggcg gataacgtaa atcgatctga ttagaaatta acacttatca ttaaaattat
     1501 cgcgttaacc ttactatgac aatgttcgca gttccgctcg agccctaata aaattatctt
     1561 ttaccgggaa tacttgcgtc aaggacatat gccacaaagt aggatggaca tttgtctcat
     1621 tcgacggtta gtaaaggctg ataataatag tagttttaca ggcggcggtg atctttcagg
     1681 ttataagatt ttgaagcgcg gttttatccc taactaccgt cacacgtcta ccgcacttga
     1741 tcagctccat tcttctgagc ttattaatcg ccctcgtgag agttctcctg gacatacccg
     1801 ccatttcagc catctcttcc tgacaaacgc ccatatttat cacgaaccca tcgggtgtgg
     1861 gctccccaac ttcctcgcac agcctcagca gcagtgaagc aagccgttcc tcaggccccg
     1921 ctagcgctgc gtttttcaga tcggcgtacg tcctcgagag ctcctcgcag agagactccg
     1981 cgagtctcag gcagacgcct ccgtctctcc ttagaaatgc aaggaaatcc tctttatcta
     2041 caaagttcag cttagattcc tccacggtcc gcgcggtaaa atcatgcgac tttccggaga
     2101 gaacagccct gactccgatc acgctcccgg ccgaaggata acccgcaatg aggccccggc
     2161 cgtcggggga atggaccgaa agcttgacgc ggcccgagca gatacaatag tttccgcgcg
     2221 gtctttcacc tttgaggaaa aggacgacat ccttcggata atcaaacctc tgcatcttgc
     2281 tctcgagctc gacaactccc tcactatcga gcccggagaa gatagtgtcc gctctcaggc
     2341 tgcatgtgct gccgctttta aatcttcgcc gcacgggcgg gttcctcccc ggattctttc
     2401 gtacagtagt ccctcggcac gtaaacacag tatggctcag atttcatata atcgcccgtt
     2461 agcgcataag ccctagccct cgagccgccg cagatgttct tgaactcgca tgcgccgcac
     2521 ttgcccttga gcctgtcgta atcccttatc tccctgaaaa ggggcgagtt cctgtaaata
     2581 tcgactatgc tcccctcctt cacgttacct gctgagagcg gcaggaatcc gctcggatac
     2641 acctcgcccg tgtgcgagac gaaaacgaag cccttcccgt cgttaatccc gcggggcgct
     2701 ctgcctatcg cgtcccgctt cctttcggca gcggcgtcta gcgggcattt gccggctctc
     2761 gattccctta ccctcgcctg cgccgcgtat ctcctgtaat gaggcgcttc cgttgacttg
     2821 atgtcatacg acaccgactt cgaaagctcg tacatcttcc cgaagacctc ttcgtattcc
     2881 tcggcgctca cgccgtcggc cgccctcccc ctccccgtcg ggacgaggaa gaatacgctc
     2941 cagagaacgg ggccgaatcc tccgatgagc ttcgctatgt tctcgaaatc gcccaggttg
     3001 tatcgtgtca cggtggtatt gacctgaatc ggtatgccct cgagggcgca gagagagagt
     3061 atctcgacgg tcctcgcgaa gctcccttcg actcccctga acgagtcgtg tatcgagggc
     3121 atggaaccgt cgaggctcac cgcgagcctc gaaatacctg cctccctgag cttgcggacc
     3181 gactcgtcgg tgaggagcag ggtcacgctc ggggacacgg tcgtccgcag tcccttctcc
     3241 acgctatacg agatgaggtc gtatatgtca tcccttttta acgggtcgcc gcccgtgagc
     3301 acgaggagag gcgatccgaa cccggctaca tcgtttatga ggttccgtcc ctgatacgtc
     3361 gtgagctcgc gcggatccct gcatctgcgc gcgtccgccc tgcag
//
LOCUS       DASSJH010000269         4787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1471921, whole genome shotgun sequence.
ACCESSION   DASSJH010000269 DASSJH010000000
VERSION     DASSJH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471921"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1245
                     /locus_tag="VFJ67_05780"
     CDS             187..1245
                     /locus_tag="VFJ67_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7289932.1"
                     /translation="MKIRSLILTAAILLGLAVPVQTVRADDSKVTIRAGHFPNITHAQ
                     GVIGQARGDFEKALGDKASIDWKLFNSGTSAIEALFAGELDITYIGPNPAVNGYIKSD
                     GEALRIVSGAASGGAGFVVRKDAGIKSAGDFNGKKLATPSIGNTQDVALRNWLGENGY
                     KLKEKGGTVEVVPLENPDQLTLFIKKEIDGAWTVEPWVSRLIYDGAGELFLDEKELWP
                     NGQYVTTHIIVNTKFLKEHPDLVKTWLETNLRITDWINNNKEEAKQVLNKELKRETGK
                     AIPQNILDAALPRIEFTYDPIASSLFQSAEDAYKLGFLGKKEPDLSNIYDLSILNGIL
                     RDRGLQEIKADSGQTAEK"
     gene            1268..1510
                     /locus_tag="VFJ67_05785"
                     /pseudo
     CDS             1268..1510
                     /locus_tag="VFJ67_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
     gene            1550..2317
                     /locus_tag="VFJ67_05790"
     CDS             1550..2317
                     /locus_tag="VFJ67_05790"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7289933.1"
                     /translation="MENVTKVFGTKEGKVTALSDINLTIRDGEFVSIVGSSGCGKSTL
                     LNMIAGLDKPTSGRILADGKRVEGPGADRVVVFQEGALFPWLTVNENVEFGLKLRGVA
                     RKIRRELAYDYLKLVGLDDKFHNSYIHQLSGGMKQRVALARALVMEPSILLMDEPFAA
                     LDIQTRESLQEELQRIWVESHKTIIFVTHNVEEAVILGDRVLVFTKNPGRIKSEHDIE
                     IERPRKPGNPEVFLNTKWITDEIREDGEDTEVREAAG"
     gene            2317..3078
                     /locus_tag="VFJ67_05795"
     CDS             2317..3078
                     /locus_tag="VFJ67_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7289934.1"
                     /translation="MKRKIILLTVFYLLLIGVWEGVASLHIWPSYLFPSPLSVVRSLI
                     AGFQDNSFTIGILESMKRVLIGYGLSIVGGVLIGLAIGRVKVLDETVGTIVLGFQALP
                     SICWLPIALLWFGLSESAIVLVVVLGSLFAVTIGVDSGVKNVPPIYLRAARTMGAGGF
                     NLYTKVIIPAAMPSIISGLKQGWSFAWRALMAGELLYVSIGLGHLLTMGRELNDISQI
                     IAVMIIIIVIGITVDRFVFTKLENRVRERWGLLNA"
     gene            3323..3985
                     /locus_tag="VFJ67_05800"
     CDS             3323..3985
                     /locus_tag="VFJ67_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289935.1"
                     /translation="MKSEDTVRIANREWENIRESLIGIADIGDLDLGALPGSGSAKKY
                     SGLYFVSSLNRLEEGFSEHGYFTPEAAYVYVNLVFIAGKKLGLEDNFAESFGSGYSWV
                     RTGFFDLDYVEDHRTVIKQLLFFQTFYPIEGDYNWSFESSEVREKLKRVFNNFLLWQN
                     NPAAYVKGLRVFREKLEPLRRGLSKALGVPIVDGPRSVRSARYSRYKKPDLKSAGGSI
                     IK"
     gene            4029..>4787
                     /locus_tag="VFJ67_05805"
     CDS             4029..>4787
                     /locus_tag="VFJ67_05805"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET7289936.1"
                     /translation="MISRIIGNTGAVVILLLILLSPASSFAQSTKQQIEELKKQIEII
                     QLQNQKQIEELKKQIEGLQTERAADQEKIAEIKTKQETVDKDAWYNNFLAKYDKGFTF
                     ESSDEKGFKFKTRFTMLAQIQGIVNDTDNKDVATNFNIRRFELRWDGYAFVPWFYYTL
                     MIDPAASSLLKDAYLTAAYKKEISPRTGQWKVPFNREELNSSSALQMVERSIVNAQFS
                     LERDRGLALQGGLGKNNNVSYSAGVFNGDGLNGTS"
BASE COUNT         1272 a         1165 c         1389 g          961 t
ORIGIN      
        1 ctgggccgag gctaatgggg tatgtataaa tattatacag ttgtatcaca tggaatgaat
       61 aagtggttac tgtttaatcg ttacgatcca tgtgcccagc cgtttctcct gtaatcatta
      121 gataatttat tttggcacgc gttttgccat tatagctccc ctaacatcga ctcaggagga
      181 aaaacaatga agataaggtc attaatttta actgctgcga ttttgctagg gcttgcggta
      241 ccggtccaga ccgtgcgtgc cgatgattcg aaagtaacca tccgcgccgg tcattttccc
      301 aacataaccc atgcccaggg tgtcataggg caggcgaggg gggatttcga gaaggcgctc
      361 ggcgataaag cctccataga ctggaagctc ttcaattcgg ggacttccgc catagaagcc
      421 ctcttcgcgg gcgagctgga tattacgtac ataggaccta accccgccgt caacgggtac
      481 atcaaatcgg acggcgaggc tctccgcata gtgtctggcg ccgcgagcgg cggcgcggga
      541 ttcgtggtga ggaaggacgc aggcatcaag tcggccgggg attttaacgg taagaagctc
      601 gcgactccga gcatagggaa tacacaggac gtcgcgctca ggaactggct cggcgagaac
      661 ggatacaagc tcaaggaaaa aggcgggacc gtcgaagtgg tgccgctcga aaatccggac
      721 cagctcaccc tgttcattaa aaaggaaata gacggcgcat ggaccgtaga gccctgggta
      781 tcgaggctca tatacgacgg cgcaggagag ctgttcctcg acgaaaaaga gctctggccg
      841 aacggtcagt acgtgaccac tcatattatc gtcaacacga agttcctcaa ggagcatccc
      901 gacctggtta aaacatggct cgaaactaat ctccggataa ccgattggat aaacaataac
      961 aaggaagagg cgaagcaggt gcttaacaag gagctaaaga gggaaacggg gaaggcgata
     1021 ccgcaaaata ttctggacgc tgcgctcccg agaattgaat tcacgtatga ccctatagcg
     1081 agctctctat tccaatcggc cgaggatgca tacaagctcg ggttcctggg aaagaaagag
     1141 ccagacctct ccaatattta cgacctttcg atattaaacg gaattctcag ggacagaggt
     1201 cttcaagaga tcaaggccga cagcggtcag acagcggaaa aataaacgtc taatacaagg
     1261 aaatcagatg catttggaaa cagtgaagaa actggaagtg ctcaagcctg acaaagtcgt
     1321 cgatctaagg gacgcggtct gccctcatcc cctcaacgtg ataagggaca ctatcagggc
     1381 gctcaacgat gacgcggtgc ttctcgcgat atcggatcac gaagccacgg cgctcgagaa
     1441 cgcgcccagg ttctgcgaga ggagagggta tccgtaccgg gtcatcgagc aggaaggctt
     1501 gtggaacata ttcattcaga agatagagaa gaaagaggcg aggctcaggg tggaaaacgt
     1561 cacgaaagtg ttcggaacga aagagggaaa ggtcaccgcc ttaagcgata taaacctcac
     1621 gatacgcgac ggagagttcg tctcgatagt cgggtcttca gggtgcggaa agtcgactct
     1681 cctcaacatg atcgcggggc tcgataagcc cacgagcgga cggatactcg cggacgggaa
     1741 gcgcgtggag ggccccggcg cggacagggt cgtagtcttc caggaggggg cgctctttcc
     1801 gtggctcacc gttaacgaga acgtggagtt cgggctcaag ctcagggggg ttgcccggaa
     1861 aataagaaga gagctcgcgt acgattactt gaagctcgtc gggctcgatg ataagtttca
     1921 caactcctat atccaccagc tctcgggcgg catgaagcag cgcgtcgcgc tcgcgagggc
     1981 gctcgtaatg gagccctcga tattgctcat ggatgagcca ttcgccgctc tcgacataca
     2041 aacgagggag tcgctccagg aggagctcca gaggatctgg gtggagtccc acaagacgat
     2101 cattttcgtt acacataacg tggaagaggc ggtgattctc ggcgacaggg tgctcgtgtt
     2161 cacgaagaac cccgggagga tcaagtccga gcacgatata gagatcgaaa gaccgaggaa
     2221 acccggcaat ccggaggtgt tcctgaacac gaaatggatc acggacgaga taagggaaga
     2281 cggtgaggat acggaagtca gggaggccgc gggctgatga aacgcaagat aattctatta
     2341 accgtttttt atctcctcct tataggcgta tgggagggag tagcctcgct tcacatatgg
     2401 ccctcatacc tgttcccgtc cccgctaagc gtcgtgaggt cactcatcgc gggctttcag
     2461 gacaacagct tcacaatagg cattctggag agcatgaaaa gggtgctcat cggttacggt
     2521 ctgtctatag taggcggcgt cctcataggt ctcgcgatcg gcagggtgaa ggtgctcgac
     2581 gaaacggttg gtacgatagt gctcggcttc caggcactcc cgagcatatg ctggctccct
     2641 atagcgctcc tctggttcgg cctcagcgaa agtgcgatag tcctcgtggt agttctcggg
     2701 tcgctctttg ccgtcaccat aggcgtcgat tccggtgtga agaacgtacc ccccatatac
     2761 ctccgggccg cgaggacgat gggcgcgggc ggattcaatc tctacactaa agtcatcatc
     2821 cccgccgcta tgccgtccat cataagcggg ctcaagcagg gatggtcttt cgcgtggcgc
     2881 gctctcatgg ccggcgagct tctctacgta agcatcggac tcggacatct cctcacgatg
     2941 gggagggagc taaacgacat aagccagatc atagctgtaa tgataataat catagtcatc
     3001 ggcataacgg tcgacaggtt cgtctttacg aagctcgaga accgagtaag agagaggtgg
     3061 gggttgctaa atgcctgagc atgtcgattt caaaaatctg atagtcccga agcaccgcgt
     3121 caagctaagg ggcacgtatt attgttgtcc tgacagcaac tagctattag ttttaaccga
     3181 caatcggaat atcccgtcaa cacacgcttt tattgaggcg cacgggagag ctgcggaaaa
     3241 tttcccggat ttgaaaagtt gatatctcaa ctttacgggg gagttctacg caaaaagcaa
     3301 aaggatatta caggagatga aaatgaaatc ggaagataca gtcaggatag caaatcggga
     3361 atgggaaaac ataagggaat cgctcatcgg gatagcggac atcggagatc tggatttagg
     3421 cgcgctcccg ggatcgggct ctgcgaagaa gtatagcggg ctctacttcg tgagcagcct
     3481 caaccgtctg gaagagggat tttccgagca cggatatttc acgcccgagg cggcgtacgt
     3541 atacgtaaac ctagtgttta tcgcgggaaa gaaactgggg ctcgaagaca atttcgccga
     3601 gagcttcggg agcggttaca gctgggtgcg gaccgggttc ttcgacctcg attatgtcga
     3661 agaccaccgg acagtcataa agcagctcct cttctttcaa accttttacc cgatcgaagg
     3721 ggactataac tggagcttcg aatcttccga agtaagggag aagctcaagc gggtgttcaa
     3781 taacttcctc ttgtggcaga acaacccagc cgcgtatgtc aaagggctga gggtgttcag
     3841 ggaaaagctc gagccgctca ggcgcgggct ctcaaaggcc ctcggcgtgc ctattgtcga
     3901 cgggccgagg agcgtaagga gcgccaggta ttcgaggtac aaaaaaccgg atctaaaatc
     3961 agccggcggg tcgattatca agtgaccggc taactgcgag aaaaatcaat ttaaggagga
     4021 taagtaagat gataagcagg atcattggaa acacgggggc ggtggtaatt ctgctcctga
     4081 tacttctcag ccctgcgagc tcgttcgctc agtcgacgaa gcagcagata gaagagctca
     4141 agaagcagat cgagatcata cagctccaga accagaagca gatcgaggag ctgaagaagc
     4201 agatcgaggg tcttcagacc gagagggcgg ccgatcagga gaagattgcg gagatcaaga
     4261 caaagcagga gacggtcgac aaggacgcct ggtataacaa tttcctggcg aagtacgaca
     4321 agggctttac gttcgaatcg tcggacgaaa aaggcttcaa gttcaagacg agattcacga
     4381 tgctcgccca gattcagggc atagtcaacg atacggacaa taaggacgtc gcgacgaact
     4441 tcaacatcag gaggttcgag ctcaggtggg acgggtacgc gttcgttccg tggttctact
     4501 atacactcat gatagacccg gctgcgagct cgctcctgaa ggacgcttat ctcaccgccg
     4561 cttataagaa ggagataagc ccgaggacgg gacaatggaa agtgcccttc aacagggaag
     4621 agcttaactc gtcctcagcg ctccagatgg tcgaaaggtc gatcgtgaac gcgcagttct
     4681 ctctcgaaag agaccgcggc ctcgcgctac agggcggact cgggaagaac aacaacgtct
     4741 cctacagcgc cggcgtcttt aacggtgacg ggctcaacgg cacgagc
//
LOCUS       DASSJH010000270         8842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_823559, whole genome shotgun sequence.
ACCESSION   DASSJH010000270 DASSJH010000000
VERSION     DASSJH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823559"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFJ67_05810"
     CDS             complement(<1..878)
                     /locus_tag="VFJ67_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289937.1"
                     /translation="MNMSEHGTEITTAGNPVIRVSTILTLLLVYLLLFYSVPSMSENA
                     ASGVYTVELKSKAGLVKIFLPDDISAGDTVSAAVALYPGGVTGEAMNSNLRILGSYVI
                     ETSFFNISASQKSIKISVPRNSAGTKMKFTLRDASMKSLDSASVPIGLSSAAVGEVET
                     PTPYDYQCPLIGQAGRTVEIKGPFDGDFATTDFKIGNKKANVIAESPRKLVFESPTDV
                     IGSVEVVLVERDVEVRRPFTCLQVLKIGEGDAVPVVSGSQTLPPARTASAGRDERSPA
                     PGVPQRELPPASQSLE"
     gene            complement(886..1611)
                     /locus_tag="VFJ67_05815"
     CDS             complement(886..1611)
                     /locus_tag="VFJ67_05815"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7289938.1"
                     /translation="MSRESSPGPSRILSFLFAFTVLFVLVFGLGVFVGKRLGRQELKI
                     TNTFEEAAPEPTAGLVEEPEEQESAEIVESPAPAAEEAAIETPPPTEPPRVEEKKSAE
                     AEVKKTEAPNRIETPPPAKPDEKLAEVTREIERELDKKRAGQKEEAKTVKPVAPQIDP
                     AGAYTVQIGSFQDQKQANSLASYLQSKGYPVFIKSMTSPDNEKWYRVRVGTFKDIESA
                     KAYGESLKNKEPKVTLVFITINY"
     gene            complement(1615..3294)
                     /gene="argS"
                     /locus_tag="VFJ67_05820"
     CDS             complement(1615..3294)
                     /gene="argS"
                     /locus_tag="VFJ67_05820"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET7289939.1"
                     /translation="MKEDIERVVLQSVESIGKKINLTDLPVEVEVEIPKRKEFGDFSV
                     NTAMVLARKMGRNPRQVAELIIENLPGEKDRLFRKIEIAGAGFINFFVREEAIVNKLG
                     DITGLGERFGTSDLGKGLSVLVEFVSANPTGYLHMGHARNAVVGDTISNILSASGYTV
                     TKEFYINDAGRQMELLGESVFARYMEIFGQNAQIPEDGYKGDYVKDIASEIKELEGDS
                     LLGKPRDESLSFAREFAREKLLGFIKKDLTDIGVDFGVWYSEKEYIHGYGENGSAPGK
                     LGEIKKRLADGGALEEKDGALWFAATRYGDTQDWVLVKSDGSPTYFLADIAYHADKFE
                     RGFQRLINVWGADHHGHVSRLKAALRALGFDDSRFNVVLIQFVRLMRHGEEVSMSKRA
                     GSYVTMRDVVSEVGADVMRFFLLMRSSESHLDFDLDLAKKESSENPVYYIQYAYARIR
                     SLFRKADEEGVEESASSLGMLTQQEELDLIKKLLLYPDVVEDSADTLAPHKIAYYLQE
                     LAADFHAYYNKFRIVDKDRALSGARLYLVRCVETVLSNGLGLLGISAPERM"
     gene            complement(3383..4009)
                     /locus_tag="VFJ67_05825"
     CDS             complement(3383..4009)
                     /locus_tag="VFJ67_05825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7289940.1"
                     /translation="MNLILIRHGETDWNRTGRCQGVADIDLNDTGRRQIEELAHSLKD
                     ADIRAVYSSDLLRALRTAREIASSHGLEVRVEKDLREMDQGELEGLLFDDIRDRYAHV
                     LREWRESPETLRLPGGETLLQVEERAWGVFERLDSLHSDETIVAVSHNLTITALLCRI
                     TGVGLRGFRNFNLRAASKNLIVSSNGSIEVSVLNDVSHLSPAESIPQF"
     gene            complement(4075..5190)
                     /gene="tolA"
                     /locus_tag="VFJ67_05830"
     CDS             complement(4075..5190)
                     /gene="tolA"
                     /locus_tag="VFJ67_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HET7289941.1"
                     /translation="MKPEDKDTTKGTSFWGIVFSAALHAAVLILVLFWGFSGTSNNNG
                     SSGPIQVSLSDLGPGGGGQGSNPANQPKAPPREPEKPKPAQQEPPPVEKKEEVKETPP
                     EPEEKPKEPEKVEVKKEEPKKEEKPKEVVKEEPEKKEVIPLETEKKEEVKKEVKKEEP
                     KKTEPPKETKKEVAEKPKPTEKPKTEAKKTTASKKKDLESEKNKVLQDIKRQRVLDEL
                     KSGPGESAQPEEEMGEEQRLAMADSGEETGESESGPGNSTEGSRSNEGGGGSSLNPVL
                     LDLYKDKVHRKISRNWRIPPGVPTDGSLVAGVFFRVDQSGRVFDVRINSSSGNTAFDE
                     YCVSAIRKSAPLPAPPSEFAKQAETEGLEVSFRNAPY"
     gene            complement(5211..5645)
                     /gene="tolR"
                     /locus_tag="VFJ67_05835"
     CDS             complement(5211..5645)
                     /gene="tolR"
                     /locus_tag="VFJ67_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HET7289942.1"
                     /translation="MGMQTGKNNGRSVMSEINVTPFVDVMLVLLVIFMVTTPILYQGV
                     DVNLPKTESRPMPSLDRERKVVVTLNSSGEIFIEKERYTLDQLRIEIRKLMAERGKNL
                     TDEDVFLRADTSVPYGTVVEVMSEIRNAGVQKIGLITEPVPH"
     gene            complement(5651..6388)
                     /gene="tolQ"
                     /locus_tag="VFJ67_05840"
     CDS             complement(5651..6388)
                     /gene="tolQ"
                     /locus_tag="VFJ67_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HET7289943.1"
                     /translation="MLEAQANNGAGQIKIVNLITDAGPVVKVVLLLLFVMSLISWTII
                     FSKSFVLKRASKRSEKFLELYNASGNFGNLYSSTKHIGGPIAEVFRAGYTEILKLRRA
                     GGAAQNPESRTSPEVIVNELGVVELVERALKRTMASETAKLEGSLIFLATTGSAAPFI
                     GLFGTVWGIMTSFIGLAGSQGVPTLQAVAPGIAEALVATAIGLAAAIPAVIAYNYFIS
                     RVKRIDIEMENFSSEFLNIVDRYIKKV"
     gene            6662..7108
                     /locus_tag="VFJ67_05845"
     CDS             6662..7108
                     /locus_tag="VFJ67_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289944.1"
                     /translation="MIKAAGLTTLAMTLLLAAVSFARAAEDRYKVVIEESYYVVKPEN
                     RDKFLSIYREKLYPFWSEMQKRGVIVDEYKLFTQRVHTLEPLWTYKTVVKFKNYESID
                     RWLELRDEVYNKLFPEGGGYEKPRKELDLITEDHWDEFIRELPLKN"
     gene            complement(7144..7761)
                     /locus_tag="VFJ67_05850"
     CDS             complement(7144..7761)
                     /locus_tag="VFJ67_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289945.1"
                     /translation="MKCQQCNNNALYQVGSEQVPLCLNCFALMQQTTDRIDEALKEEI
                     NYLHDSIDYTIFGFRAGPRYPTKRPVLLSGGTVNHNHIAINNSQVGVLNTGNIKNLNQ
                     TIDSLYAASQKELAENIRKFSNAILADENLTDDQKTQVLESLDFITKELFQKSENRKR
                     TIVSTLFGVISKAVELTANTLVVWQALYPMLESFSVNSTEDYGSR"
     gene            complement(7790..8389)
                     /locus_tag="VFJ67_05855"
     CDS             complement(7790..8389)
                     /locus_tag="VFJ67_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HET7289946.1"
                     /translation="MAHAIDMPSESPQKIKHHLLQLQKKGFLIIDRAKGMMERTDTKP
                     SWATGLLHKTNLLFSIPIVGTANCGPATIFAEENFQGFLRVSSRLIGRSRPDGLYAVK
                     AGGSSMNRVNINGGSIDDGDYVIIDGNDLTARDKDVVVAIIENKATIKRFIYDKNNYQ
                     IVLRSDSSFDYEPIYIHPEDEFRISGKVIGVIKNPKISD"
     gene            complement(8646..>8842)
                     /locus_tag="VFJ67_05860"
     CDS             complement(8646..>8842)
                     /locus_tag="VFJ67_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HET7289947.1"
                     /translation="GPVGIDSIAAAVSEDRDTIEEVYEPFLIRQGLVAKTPRGRVVTP
                     LAYSHLNMPLPRAQKGLFGD"
BASE COUNT         1841 a         2462 c         2136 g         2403 t
ORIGIN      
        1 aactcgagcg actggctggc cggcggaagc tccctttgcg gaacaccagg cgcgggtgac
       61 cgctcgtccc ttccggcaga cgccgttctc gcaggcggaa gcgtctgtga gccgctcacg
      121 accggtaccg cgtctccctc gcctatcttt aaaacttgca ggcatgtgaa cggcctcctc
      181 acctccacat ccctttcgac gagaaccacc tctacggagc cgattacgtc cgtagggctt
      241 tcgaagacaa gcttccgtgg agactcggcg atgacgtttg cttttttatt gccgatcttg
      301 aaatcggttg tcgcgaagtc cccgtcaaag gggcctttta tctccacagt cctcccggcc
      361 tggccgatga gcgggcactg gtaatcgtaa ggtgtcgggg tctctacttc acctaccgcc
      421 gccgaagaga gacctattgg taccgatgcc gagtcgagag acttcatcga tgcgtccctg
      481 agcgtgaatt tcatcttggt cccggcagag ttccttggga cgcttatctt tatggacttc
      541 tgtgaggcgg agatgttgaa aaagctcgtt tcgatgacgt agctgccgag tatccggagg
      601 ttactgttca tagcctctcc ggtaacccct cccgggtaaa gcgctaccgc ggctgatacc
      661 gtgtcccctg cggatatgtc gtcaggaagg aatatcttta cgagaccggc tttcgatttg
      721 agctcgaccg tgtatacgcc gctcgcggcg ttttcggaca tcgacgggac cgaataaaag
      781 agcaaaaggt aaacgaggag cagagttaga atcgtcgaga cccggatgac cgggtttccg
      841 gctgtcgtga tttctgttcc gtgctcggac atgttcatga ctgcatcagt aatttatcgt
      901 gataaatacg agcgttactt tcggctcctt attcttcagt gattctccgt atgccttggc
      961 gctctcgata tctttaaatg tccctaccct gaccctgtac catttctcgt tatcgggcga
     1021 cgtcatggat ttgataaaca cggggtatcc tttcgactgc agatagctcg cgaggctgtt
     1081 cgcctgcttc tggtcctgaa aagaccctat ctgtactgta tatgcgcccg cgggatcgat
     1141 ctgaggcgca accggtttca cggtcttcgc ctcttccttt tgcccggcac gtttcttgtc
     1201 gagctcgcgc tctatttcac gtgtaacttc ggctaatttt tcgtcaggct tggcaggggg
     1261 gggcgtttcg attctattcg gagcctcggt tttttttact tcagcttccg cgctcttctt
     1321 ttcctcaaca cgcgggggct cggtaggtgg cggcgtctcg atagccgctt cctcggctgc
     1381 cggggctggg ctttccacta tttccgcact ctcctgctcc tccggttcct cgaccagtcc
     1441 ggcagtcggc tcgggtgcag cttcttcgaa cgtattggtt atcttgagct cctgacggcc
     1501 gagtctcttg ccgacgaaca ctccgaggcc gaaaaccagt acgaagagta cggtaaaagc
     1561 gaacaggaac gaaagtatcc tgctcggtcc cggtgaactt tccctgctca tacgttacat
     1621 cctctccggc gccgatatgc ctagaagtcc caatccgttg ctgagcactg tctccacgca
     1681 tctcacgaga tagagccttg ccccgctcag cgcgcggtct ttgtcgacga tcctgaattt
     1741 attgtagtag gcgtggaagt ccgccgctag ctcctgaaga tagtatgcga tcttgtgcgg
     1801 agcgagcgta tccgccgaat cctcgaccac gtccggataa agaagcagtt ttttgatgag
     1861 gtcgagctcc tcctgctgcg taagcatgcc gagagacgaa gccgactcct caacgccctc
     1921 ttcgtccgct ttcctgaaga ggctcctgat ccgcgcgtat gcgtactgga tataatagac
     1981 cgggttctcg ctcgattcct tcttcgcgag atcgagatcg aagtcgagat ggctctcgga
     2041 gctccgcatc aggaggaaga agcgcatcac gtcggctccg acctcgctca ccacgtcccg
     2101 catcgtgacg tagctccctg cccttttcga catggacact tcctcaccgt gcctcatgag
     2161 gcgtacgaac tggatgagca ccacgttgaa cctcgaatca tcaaagccga gcgcccggag
     2221 tgcagccttc agcctggata cgtgcccgtg atggtcggcc ccccagacgt tgatgaggcg
     2281 ttgaaaaccc ctctcgaact tatcggcatg gtacgcgata tcggcgagga aatatgtagg
     2341 gcttccgtcg ctcttgacga gcacccagtc ctgcgtgtcg ccgtatctgg tggcggcgaa
     2401 ccacagggct ccgtccttct cttcgagagc gcccccgtcc gcgagacgct tttttatctc
     2461 gccgagcttg ccgggcgcgg agccgttctc gccgtacccg tggatgtatt ccttttcgct
     2521 gtaccagacg ccgaaatcga cgcctatgtc cgtaaggtct ttttttatga agcccagaag
     2581 cttctccctc gcgaattccc tcgcgaaaga gagggattcg tccctgggct tacctagaag
     2641 cgaatcgcct tcgagctctt ttatctcgga cgcaatgtcc tttacataat cgcccttgta
     2701 tccgtcctca ggtatttgcg cgttctgtcc gaagatctcc atgtatctcg cgaataccga
     2761 ctcgccgaga agctccatct gcctccccgc gtcgttaatg taaaattcct tcgtgactgt
     2821 ataacccgag gccgagagta tattcgagat cgtgtctccc acgaccgcgt tcctcgcgtg
     2881 acccatgtgg aggtaacccg tcgggttcgc gcttacgaat tcgacaagca cggagaggcc
     2941 tttcccgagg tccgaggtcc cgaaccgctc gccgagcccc gttatatccc cgagcttgtt
     3001 tactatggct tcttccctga cgaagaaatt aatgaacccg gcccccgcga tctcgatttt
     3061 tctaaagagc cggtccttct cgccggggag gttctcgatt atgagctccg ccacctgccg
     3121 cgggttcctg cccatcttcc tcgcaagcac catggcggta ttaacagaaa aatcgccgaa
     3181 ctccttacgt ttcgggattt cgacctctac ctcgacgggg aggtcggtga ggttgatttt
     3241 cttacctatg ctctcgacag attgtaaaac gaccctctca atatcttcct tcatatcgtt
     3301 atcaggaaat tacaccatat atccgtggga gttacaagaa aggcgcgata ccgggatgtc
     3361 cgtccgggaa atatcatgat aatcagaatt gtgggatcga ctcggccggg gacaggtggg
     3421 atacatcgtt gagaacactg acctctatac tgccgtttga ggaaacgatc agattcttcg
     3481 acgcggccct caggttgaaa ttacggaatc ctcttaatcc gacgcccgta atcctgcaca
     3541 gaagcgccgt gatcgtgagg ttatgactaa ctgcgactat agtctcgtcc gaatgaaggc
     3601 tatcgagcct ttcgaatacg ccccacgccc tttcctccac ttgcaggagc gtttccccgc
     3661 cgggcagtct tagcgtctcc gggctctccc tccattccct gagcacgtgg gcgtatctgt
     3721 cccttatatc gtcgaacagg agcccttcga gctccccctg gtccatttct ctcaggtcct
     3781 tttccaccct gacctcgagg ccgtggctgg aggcgatctc cctcgccgtg cggagcgccc
     3841 ggagaaggtc gctcgaatac acggccctta tgtcggcgtc ctttagcgag tgagcgagct
     3901 cctctatctg ccttctgccc gtgtcgttga ggtcgatatc ggccacaccc tgacacctgc
     3961 ccgtcctgtt ccagtccgtc tctccgtgcc gtatgagtat tagattcata ttttggttgt
     4021 tacagaaagc tccggaatgt ttaacccggc ggccgtaatc ccgccccgta cggctcagta
     4081 aggcgcgttc ctgaatgata cctcgagccc ctctgtctcg gcttgttttg caaactccga
     4141 tggcggcgct ggaagggggg ctgatttacg tatcgcactc acgcaatatt cgtcgaacgc
     4201 cgtattgccg gaggatgaat ttattctgac gtcgaatacc ctgccgctct gatcgaccct
     4261 gaagaagacg cccgcgacga gactgccgtc cgtaggcact ccgggcggta tcctccagtt
     4321 cctgcttatc ttcctatgga ctttatcctt atataaatcc aacaagaccg ggttcaggga
     4381 cgacccgccg ccgccctcat tactccggga cccttcagtc gaattacccg ggcccgactc
     4441 cgattccccc gtttcctctc ccgaatctgc catcgcgagc ctctgctctt cccccatctc
     4501 ctcctcgggc tgagcactct ctccggggcc gctcttgagc tcatccagga ccctctgtct
     4561 ctttatgtcc tggaggacct tgttcttctc gctctcgagg tcttttttct tgcttgcagt
     4621 cgttttcttg gcttcggttt tcggtttctc tgtaggcttg ggtttttcag ccacttcctt
     4681 tttcgtttct ttgggcggct ccgtcttctt cggctcttcc ttttttactt ccttcttcac
     4741 ttcctctttt ttctcggtct cgagcggtat gacttccttt ttctcgggct cctccttcac
     4801 gacctctttg ggcttctctt cctttttagg ttcttctttt ttaacctcga ccttctccgg
     4861 ctctttcggc ttctcttccg gctcgggcgg agtctccttg acctcctcct tcttttcgac
     4921 cggtgggggc tcttgctggg caggcttcgg cttctcaggc tcccgtggcg gcgctttcgg
     4981 ctgattcgcg ggattagagc cctgtccccc tcccccgggc cccaagtccg agagggatac
     5041 ctgtataggc ccgctcgatc cgttattgtt ggaggtgccg ctgaatcccc agaacagcac
     5101 gagaatcagc acggccgcgt ggagagcggc tgaaaatact atcccccaaa aagatgtgcc
     5161 tttggttgta tctttgtctt ccggtttcat ttccccgctt acctctggcc ttagtgcgga
     5221 acaggctctg ttataagccc gatcttctgt acccccgcgt ttcttatctc cgacatgacc
     5281 tcgacgaccg tgccgtacgg gacgctcgta tcggctctca ggaacacgtc ctcgtccgta
     5341 aggttcttgc ccctctcggc catgagcttt cttatttcga tacgcagctg gtcgagcgtg
     5401 taccgttctt tctcgatgaa tatctctccg ctgctgttga gcgtgaccac gacctttctc
     5461 tccctgtcga gcgacggcat cggccgggac tccgttttcg gaaggttcac gtccacaccc
     5521 tgatagagta tcggggtcgt gaccatgaat atgacgagaa gcacgagcat cacatccaca
     5581 aacggcgtta cgtttatctc cgacatcacg gagcgtccgt tattcttacc tgtctgcatt
     5641 cccatgttcg ctataccttc ttgatatatc tgtccactat gttgaggaac tcagaggaga
     5701 agttctccat ttctatgtcg atcctcttca ccctgctgat gaagtagttg tatgcgataa
     5761 cggcaggtat ggccgcggcg agacctatcg cagtcgctac aagggcttcg gctatgcctg
     5821 gggcaacagc ctgaagcgtg ggcacgccct ggctcccggc gagcccgatg aaggaggtca
     5881 tgatacccca tacagtgccg aagagtccta tgaacggcgc tgcgctgccc gttgtggcca
     5941 ggaaaatgag cgagccttcg agcttggccg tttccgacgc catggtacgc ttgagcgccc
     6001 tctcgacgag ctcgacgact ccgagctcgt tcactatcac ctccgggctc gtcctcgatt
     6061 ccgggttctg agccgctccg cccgccctcc tcagcttcag tatctccgta tatccggccc
     6121 tgaagacctc cgctatcggc ccccctatgt gcttggtcga ggagtagagg ttcccgaaat
     6181 tgccgctcgc gttgtagagc tcgaggaact tctccgacct cttggacgcc cttttcagga
     6241 cgaaggactt agaaaaaatt atcgtccagg aaatgagcga catcacgaac agaaggagca
     6301 gcacgacctt cacgaccggt cccgcatccg tgatgaggtt aacgattttt atctggccgg
     6361 cgccgttatt cgcctgcgcc tctaacatta aatatatcca gtttgtcata tgtgctgacc
     6421 cgctgaaaag ttggattttg gatttgaacg aaaatcaatt cttctgactg ttaagtacga
     6481 atgagccgca tttaagattt ggtattaagt tttccatact tagaaactaa aatcaaataa
     6541 tattcacatg aaaaccgcga tccggcgggt ttgcggcggg gtgaggccgg gatgtataat
     6601 atgagtcccg aaaagggcgg gagaggttcc cggaacttta aagggagggt agataaaaag
     6661 aatgataaaa gcagcgggcc tcacaactct cgcaatgaca ctgctcttag cggcggtatc
     6721 tttcgcccgg gccgccgagg acaggtataa ggtcgtcatc gaggagagct attacgtggt
     6781 caagcctgaa aacagggata agttcctcag tatataccgc gaaaagctct accccttctg
     6841 gagcgagatg cagaaacgcg gcgtcatcgt cgatgagtat aagctcttca cgcagcgggt
     6901 ccacactctc gagccgctct ggacgtataa gacagtcgta aaattcaaga attacgagtc
     6961 gatagacagg tggctcgaac tccgggacga ggtttacaac aagctctttc cggagggagg
     7021 cggttatgag aagccgagga aggagctcga tctcatcacg gaagaccact gggacgagtt
     7081 cataagggag ctgcctctca agaattaata tatagctata tcttggcctt gagggggaat
     7141 tagttacctg cttccataat cttcggtcga attaactgaa aaactttcca acattggata
     7201 caaagcttgc cagaccacaa gcgtgtttgc agttaattca actgcttttg atattacacc
     7261 aaaaagagta ctgactattg tcctctttct attctcagat ttttgaaata gctcttttgt
     7321 gataaagtcc aaactttcga ggacttgagt tttttgatca tcagtcagat tctcgtcagc
     7381 taaaatagca ttagagaatt ttcttatatt ttccgctagc tctttctgag atgccgcata
     7441 taaactatct atcgtttggt tcaggttttt aatattacca gtatttagaa ctccgacttg
     7501 actattattt atggcgatat gattgtgatt tacagtcccg ccagaaagca ataccggtcg
     7561 tttggttgga taacgtggtc ctgcacgaaa cccaaagata gtgtaatcaa tactgtcatg
     7621 aagataattt atttcttcct ttaatgcttc atctattcta tctgttgttt gttgcattag
     7681 tgcaaaacaa ttcaagcata atggcacttg ttctgatcct acttgatata acgcgttatt
     7741 attacattgt tggcatttca tatttttaac cttgcatgcg cattaaatct taatcgctga
     7801 tcttaggatt tttaattaca ccaataactt tgccacttat tctaaattca tcttcaggat
     7861 gaatatatat cggttcataa tcgaatgatg aatccgagcg caaaacaatt tgataattat
     7921 ttttgtcata gataaaacgt ttaatcgttg ctttgttttc aatgatagcg acaacaacat
     7981 ctttgtcccg agctgttaga tcatttccat cgatgattac ataatctcca tcgtcgatgc
     8041 ttccaccatt gatattaacc cgattcattg atgaaccacc cgctttgaca gcatataaac
     8101 catcaggtct cgagcgtcca attagcctac tcgaaactcg gagaaaccct tgaaaattct
     8161 cctctgcaaa aattgttgca ggtccgcaat tagcagtccc gacaattggt attgagaaaa
     8221 gaagattcgt tttgtgaagc agtcctgtag cccaacttgg cttagtatca gtgcgttcca
     8281 tcattccttt agctcgatcg ataattaaga agcctttctt ctgcagttgt aatagatgat
     8341 gctttatttt ttgtggagac tcacttggca tgtcaatggc atgtgccatt tctcgcaagg
     8401 tcatcttagc cagattttct ttcttagaga gctttaagag tttttcttgg atgggatgcg
     8461 cacgttgaat atatcacaaa gccttaaatt attcaagtcc gtaaaacttg acataataca
     8521 tatcaaaaaa agcaaagccc aatggtttct ttttatagtt cccgaggtat attgggatgt
     8581 tagcccggaa tatcgaacct ctcaaatatt gatgctcaga cattaatatt tattgtagat
     8641 acgggctaat ccccgaacag ccccttctgc gcacggggta gaggcatgtt gaggtgggaa
     8701 tacgcaagcg gcgtgactac cctgcccctg ggagtcttgg ctactagccc ctgccggatg
     8761 agaaacggct catagacttc ttctatagtg tccctgtcct cgctcacggc ggcggctatc
     8821 gagtctatgc ccacggggcc tc
//
LOCUS       DASSJH010000271         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1251187, whole genome shotgun sequence.
ACCESSION   DASSJH010000271 DASSJH010000000
VERSION     DASSJH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251187"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFJ67_05865"
     CDS             complement(<1..676)
                     /locus_tag="VFJ67_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET7289948.1"
                     /translation="MSDSSDKLRALSTLKTSAGETGYYSLKSIEKTLGKDISRLPVSI
                     KILLENVLRNYDGTVINEGHLNALAGWSPDNGSAEEIPYCPARVILQDFTGVPCVVDL
                     AAMRTVVNRLGGNPDKINPIVPVDLVIDHSVQVDYFANDRAFKLNVEREYERNGERYA
                     FLRWAQKAFDNFRVVPPGTGIVHQVNLECLASVVTSANINGKTIAYPDTLVGTDSHTT
                     MINGLSV"
     gene            complement(892..2124)
                     /gene="fabF"
                     /locus_tag="VFJ67_05870"
     CDS             complement(892..2124)
                     /gene="fabF"
                     /locus_tag="VFJ67_05870"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7289949.1"
                     /translation="MKRRVAVTGIGLVTPLGIGNKETWDAVCAGRSGVRRISKFDPSE
                     HKTQIAAEINGFDPELFMNPKEARKTDAFIQYAVASAKLALEDSGLEITEELSPHTGT
                     IIGSGIGGMETYVNTVLTMAEKGPGRLSPFFITNIINNMAAGYVSIFFNAKGPNCCTT
                     TACAASAHAIGYAAMAIRNGEADAMFAGGTEAPCIPLTFAGFNAMRALSTRNDEPERA
                     SRPFDKERDGFIIGEGSGMLILEEMERAKKRGAKIYAEIVGYGMSSDASHITAPSLDG
                     PERCMLAALKDGGINPDEIEYINAHGTSTPLNDSNETNAIKQVFGQRAYKIPVSSTKS
                     MTGHLLGAAGAVEAAFTVLALDQGILPPTINQEFPDPECDLDYVPNEARKADIKTALS
                     NSFGFGGTNASLIFRKID"
     gene            complement(2124..2369)
                     /gene="acpP"
                     /locus_tag="VFJ67_05875"
     CDS             complement(2124..2369)
                     /gene="acpP"
                     /locus_tag="VFJ67_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7289950.1"
                     /translation="MAQEKEILAKVKEMVAGQLGKTEDEISPESSFIEDLGADSLDLV
                     ELIMSMEDEFGLEISDEDAESIITVQDAVNFILERKK"
     gene            complement(2390..>3016)
                     /gene="fabG"
                     /locus_tag="VFJ67_05880"
     CDS             complement(2390..>3016)
                     /gene="fabG"
                     /locus_tag="VFJ67_05880"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET7289951.1"
                     /translation="EPAALEVVAAIEKSGGSASVSGFDVSDFDTVQKKVEETIAEYGG
                     IHILVNNAGITHDTLLMRMNKEDWDRVITTNLTGVFNCTKAVTRTMIKQRSGRIVNIT
                     SVVGEMGNSGQTNYAASKAGIIGFTKAAARELAQRSVTVNAISPGFIETDITNELPEE
                     IRRQYIEKIPMGRFGRPEDIAGVVAFLTSDEASYITGEVIRVNGGIYT"
BASE COUNT          531 a          872 c          825 g          788 t
ORIGIN      
        1 gtacgctgag gccgtttatc atggtcgtat gtgaatcggt tcctactaat gtgtcggggt
       61 aagcgatggt ctttccgttt atattcgccg aggtgacgac gctcgcgagg cattcgaggt
      121 tcacctggtg gactatcccc gtgccgggag ggactaccct gaagttatcg aacgcctttt
      181 gcgcccacct taaaaacgcg tacctctcgc cgtttctctc gtattcgcgc tcgacgttca
      241 atttaaaggc tctatcgttc gcgaagtaat cgacctgaac ggagtggtct attacgagat
      301 cgacggggac tatggggttt atcttgtcgg ggttgccgcc tagcctgttg acgacggtcc
      361 tcatcgccgc caagtcaacg acgcaaggta cgccggtaaa gtcctggagt atcacgcgcg
      421 cagggcagta gggtatctcc tccgcgctcc cgttatcggg gctccatccc gcaagcgcgt
      481 tcaggtggcc ctcgtttatc actgtcccgt cgtagttcct gagtacgttt tcgagcagaa
      541 tcttgattga cacggggagg cgggaaatgt ccttccccag agttttttca atagatttca
      601 gtgagtaata acctgtttcg cccgcgcttg ttttaagcgt agaaagggct ctcagtttat
      661 cgctgctatc gctcataatt attctcctta gctagctggt taagcggaag gttttccata
      721 acattctaca gttgaatcgg gacaaatcaa gccgaagcgc cccgtaaatc catgtgaatt
      781 taaccgggcg tcagtgcgct cgaagcggat cggattaacg gggcaatccg cgcccttaac
      841 gcttccggta cggtgtatga tccgggatct gtgggttgag gagctttccg ttcagtctat
      901 ctttctgaat atgagcgagg cgttcgttcc cccgaagccg aacgaattgc ttagcgcggt
      961 ctttatatcc gcttttctcg cttcgttggg gacgtaatcg aggtcgcatt cggggtcggg
     1021 gaattcctgg ttgatcgtcg ggggcagtat cccctgatcg agcgcgagca ccgtgaaagc
     1081 ggcctctacg gctcctgccg cgccgagcaa gtggccggtc atggatttcg tcgagcttac
     1141 cggtattttg taagccctct ggccgaatac ctgcttgatc gcgttcgttt cgttcgaatc
     1201 gttgagcggg gtcgatgtcc cgtgcgcgtt tatgtactca atctcgtccg ggttgatccc
     1261 accgtccttg agcgccgcga gcatgcaccg ctcgggcccg tcgagagacg gggccgttat
     1321 gtggctcgcg tcgctgctca tgccgtaccc gactatctcc gcgtaaatct tcgcgcctct
     1381 ctttttggcg cgctccatct cctcgagtat gagcatcccc gagccttcgc cgatgatgaa
     1441 gccgtctctt tccttatcga acgggcgcga ggcgcgctcg ggctcgtcgt tacgcgtgga
     1501 aagggcgcgc atggcgttga accccgcgaa cgtgagcggt atgcacgggg cctccgttcc
     1561 gcccgcgaac atggcgtcgg cctcgccgtt cctgatggcc atggccgcgt agcctatggc
     1621 gtgtgcgctc gccgcgcagg cggtcgtcgt gcagcagttg gggcccttgg cgttgaagaa
     1681 tatggatacg tatcctgccg ccatgttgtt tatgatgttc gtaatgaaga acggggagag
     1741 gcgtccgggg cccttctcgg ccattgtgag cacggtattg acgtaggtct ccatgccccc
     1801 tatgccggag ccgattatgg tccccgtgtg cggggaaagc tcttccgtta tctcgagtcc
     1861 cgaatcctcc agggcgagct tggcggaggc gaccgcatac tgtatgaacg cgtccgtctt
     1921 cctcgcctct ttcgggttca tgaagagctc ggggtcaaac ccgtttatct cggccgcgat
     1981 ctgcgtcttg tgctcggagg gatcgaattt cgatatcctc cgcacgccgg agcggccggc
     2041 gcacaccgcg tcccaggtct ctttattccc tattccgaga ggagttacga gcccgatgcc
     2101 cgtaacagct acccttcttt tcattacttt ttacgctcca gtataaagtt gaccgcgtcc
     2161 tgaaccgtta ttatggattc cgcgtcctcg tccgatatct cgagtccgaa ctcgtcttcc
     2221 atggacatga tgagctcgac gaggtcgagc gagtcggcac ccaggtcctc aatgaatgaa
     2281 gattcgggcg atatctcgtc ctctgtcttg ccaagctgac ccgccaccat ctccttgact
     2341 ttcgcgagaa tttctttctc ctgagccatg gtaaatacct cctttgatat tatgtatata
     2401 ttccgccgtt aaccctgata acctcgcccg tgatataaga cgcctcgtcc gaggtcagaa
     2461 aagcgaccac gccggcgatg tcctcgggcc ggccgaacct gcccatcggt atcttttcta
     2521 tgtactgcct tcttatttct tcaggaagct cgttcgttat atccgtctcg atgaaccccg
     2581 ggcttatcgc gttcactgta accgacctct gcgccaattc cctggccgcc gccttcgtga
     2641 agccgattat gccggccttt gacgcggcgt aattcgtttg gccggagttg cccatctccc
     2701 ccactacgga ggtgatgttt actatccttc ccgagcgctg ctttatcata gtacgcgtaa
     2761 cagcttttgt acaattgaac actcccgtga ggttggtggt gatgaccctg tcccagtcct
     2821 ccttgttcat tctcatgagg agcgtgtcgt gagttatgcc cgcgttgttg acgagaatgt
     2881 gtatcccgcc gtattccgcg attgtctctt ccaccttttt ctgcaccgta tcgaagtccg
     2941 agacgtcgaa cccggagacg gaagcgctcc ctccggattt ctctatagcg gccacgacct
     3001 cgagggccgc gggctc
//
LOCUS       DASSJH010000272         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1578010, whole genome shotgun sequence.
ACCESSION   DASSJH010000272 DASSJH010000000
VERSION     DASSJH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578010"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFJ67_05885"
     CDS             complement(<1..964)
                     /locus_tag="VFJ67_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289952.1"
                     /translation="MSCDERNGGDTLPPDPKDWVCKSSEIGPTPEEIEQYCSTADPGL
                     PAPEFLRNPPPLSSLEDKNIYDTEMQDFLRVKGYVNELGWPGDMNWRLTGPYVGPIGS
                     GQAYGVHPAVRLYYSPEVIEWLCGGRQGEIPDGAVIVKEMHSINQSLDIALDSEGCMV
                     INADIEPNSWTIMVKQKDASPDGWYWANYSTLPEPPVAAWAVGNPPIIDRSAFTSEEF
                     LGGVPDEPNPLWYPTGYVYESDNKIPDVVGPYSEYGNYCVNCHASAEKEQTFSSLDNI
                     LGPGLRYKHYGPLGDGGTRRVSTSELPVSVPEVAGLRITPSVNKA"
     gene            1259..1756
                     /locus_tag="VFJ67_05890"
     CDS             1259..1756
                     /locus_tag="VFJ67_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289953.1"
                     /translation="MLAVISSEAGDDAAVTGKTYDSPYAYCQAVGTIDNPGKEYTGPA
                     VPESIAKALKKEWGSPESAPLDSFIRGTFWRCMDGKVYACNVGANLPCDEKADVSKDP
                     TQGMKDYCKENAGSDFIPMYVTGHSTIYDWKCDGTTPVAGKPFAEVDKQGYQKGIWYE
                     IQPAE"
     gene            1794..2501
                     /locus_tag="VFJ67_05895"
     CDS             1794..2501
                     /locus_tag="VFJ67_05895"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF004079.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET7289954.1"
                     /translation="MSIHNRAIGIIGSMDAEISHYLKNSELERETSWNGITFYETGLF
                     GKKTVVVKSGVGKVFAAMVCERLIDEFNPGAVVFTGVAGALNRRLDIGDVVLSETCIQ
                     YDLDAEALGFPRGEIPYTHLREFKADEALLNIAGSTKLKGNKVITGTILTGDQFMTTK
                     ETKEHKYLTEELHGDVIEMEGGSIAQVCTLNKIPFIIVRTVCDRADGTAAADFNKFLP
                     LAARNSLDVVKNLLQNY"
BASE COUNT          660 a          694 c          722 g          575 t
ORIGIN      
        1 ctgctttgtt gacagaaggc gttatacgga gcccggccac ctcggggacg gatacgggta
       61 attcggatgt gcttacacgc cgggtcccgc catcgccgag cggcccgtaa tgcttgtacc
      121 tcagcccggg gccgagaata ttatcgagcg acgaaaatgt ctgttccttt tccgcagaag
      181 cgtggcagtt gacgcagtag tttccgtatt cgctgtaggg accgaccaca tccggtatct
      241 tgttatcgct ctcatataca tatcccgtcg ggtaccagag cggattcggc tcgtccggca
      301 ctcccccgag gaattcttcg ctcgtgaatg cggagcggtc gatgataggg gggttcccca
      361 ccgcccaggc cgcaacaggc ggttccggca gtgtcgagta attcgcccag taccacccgt
      421 cgggagacgc gtccttttgc ttgaccatta tcgtccacga attgggctcg atgtcggcgt
      481 tgatgaccat gcacccctcg gagtcgagcg cgatatcgag cgactggttt atcgaatgca
      541 tctccttcac tatgaccgct ccgtccggga tctcgccctg ccttccgccg cacagccatt
      601 ctattacttc gggggaatag tagagtctga cagcaggatg aacaccgtaa gcctgcccgc
      661 ttcctatagg gcctacgtaa ggaccggtaa gacgccagtt catgtcgccc ggccagccca
      721 gctcgttgac gtaccctttc actctcagaa aatcctgcat ctcggtgtca tagatatttt
      781 tatcctcgag gctcgaaaga ggtggcgggt tccggagaaa ttcgggagca ggcagccccg
      841 ggtcagccgt cgagcaatac tgctcgatct cctcgggtgt aggcccgatc tcggagcttt
      901 tgcataccca gtccttgggg tcgggcggga gcgtatctcc gccgttcctt tcatcacatg
      961 acattaacgc cattaaaaag agggatagga cggatatgta caaagccgta atcttcatat
     1021 tgccgaagta tatagctgcc cataaaaatt tgatagacag ggtactaaaa aaatgaatgc
     1081 gttttatgtc ttatagggat attgattctt attcgattta tttaagctta tacgccggat
     1141 gcgctataat tgttatatac cgacttgatt gaacaatcgc aggtgaagcg cggcgagcaa
     1201 aggaggaagg atgaaaaccc ataacattct gtcattatta gtcatacctg ctgtattaat
     1261 gttggccgta atatcatccg aagcaggcga tgatgcagcg gtcacgggca aaacgtacga
     1321 cagcccttac gcctattgcc aggctgtcgg cacgatcgac aatcccggaa aggaatacac
     1381 ggggcccgca gtacccgagt ccatagcgaa ggcgctgaaa aaagaatggg gctcgcccga
     1441 atcagccccc ctcgactcgt ttatcagggg aacgttctgg cggtgcatgg acgggaaggt
     1501 ttacgcatgc aacgtcggcg ccaacctgcc gtgcgacgaa aaagcggacg tgagcaagga
     1561 cccgacccag gggatgaaag attactgcaa ggaaaacgcc ggctccgatt tcataccgat
     1621 gtacgtaacc gggcactcga ctatatacga ctggaagtgc gacggaacga cgcccgttgc
     1681 cgggaagccg ttcgccgagg tcgacaagca gggttaccag aaaggaatct ggtatgagat
     1741 tcagcccgct gaatgaaacg cactgtgtat aacgactttc aattgctttt atcatgagca
     1801 tacataatag agctatcggc attatagggt ccatggatgc cgagatcagc cactatctca
     1861 agaattcaga gttggagaga gaaacgagct ggaacggtat tacgttctac gagaccggac
     1921 tcttcggcaa aaaaaccgtc gtcgtcaaat caggtgtcgg caaggtgttc gccgcaatgg
     1981 tatgcgagcg gcttatagac gagttcaacc ccggcgccgt tgtattcacc ggggtagccg
     2041 gcgctcttaa ccgcaggctc gacattgggg acgtcgtcct gagcgaaacc tgcatccagt
     2101 acgacctcga cgccgaggcg ctcggcttcc cgcggggcga gataccttac acgcacctca
     2161 gggaattcaa ggccgacgag gctctcctca atatcgcggg cagcacgaaa ttgaagggca
     2221 acaaagtaat tacgggtacc atactgactg gagaccagtt catgacgacg aaagagacga
     2281 aagagcataa gtatctgact gaagagcttc acggagacgt tatagagatg gaaggcggct
     2341 ctatcgcgca ggtatgcacg ctcaacaaga ttccattcat aatagtgagg acagtctgcg
     2401 atagagcgga cgggacggcg gccgcggatt tcaacaagtt cctcccgctc gcggcgagga
     2461 attcgttaga tgtcgtaaag aacctgctcc agaattattg agaaagcaca aacgagaatt
     2521 attcgattat ggtcggaaca aagaaagtaa accagccggt atctaattag ccccggagct
     2581 gaacgcctcg ccatcggccg acccggtaaa catccgtatc agaatctctt cctcatcgtg
     2641 agccggtttt t
//
LOCUS       DASSJH010000273         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1143571, whole genome shotgun sequence.
ACCESSION   DASSJH010000273 DASSJH010000000
VERSION     DASSJH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143571"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2273
                     /locus_tag="VFJ67_05900"
     CDS             <1..2273
                     /locus_tag="VFJ67_05900"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HET7289955.1"
                     /translation="VDEGILQLIDQKTADPFTFFYRKLALEVNSYDTFSLLLPDVTLG
                     LSPPGGGFGARKEMQFLSTQGIRRVKPVAYWSGVLTADAEGKASFKFDMPQFQGAVRI
                     MAVAVRGEEFGSSEKFTRVKSPIVMLPTAPRFFSLNESVIIPVSLRNDTKKDGQFTTN
                     MTVSGPGTVAPMNSGDQKEKPAEGAKEAKIDSDIPDGSEKTIYFVVDTADETGAITLN
                     ITASGNGESTSFDMDVPVLADLPASRTESVGSLKTADTELAIENLESYRTGTIHRELY
                     VSKLPLIQFSGKLEYLLQYPYGCLEQVTSRVFPLIYLSDIAKEIDPDVFKKADPAAMV
                     QEGIRQIGTMQVQGGGFSLWPGGVEPEPWVSIYAAHFLVEARRAGYFADDSLYSGAIE
                     YLKSVAKAKPDYSTGELERAVYALYVLSRDGKPDLSTMDFIRDRHIGELTVESKALLG
                     GAYAAAGNAAALEELLKGLNDVEEVTRETGGNFNSTIRNRAILLLAILDVNPDDPRVP
                     ALVERLSRDVQLNAWWTTQESSFALLAIGQFVKKQSETPPYTGKVYLGDEELATFSSE
                     ATLSLPDVTGDKPLSIKMDDGFKEGSAYYTLLTSGIPTRKGFKPVAEGLNVVREFLTR
                     DGAPLDPGNIVQGDLVVVRTKLQSGSGKLENVVIENLLPSGFEVENPRLKTTETLSWA
                     TGDSVEPEYLDIRDDRILIFTDLPDAKWYNYYALLRVVNPGTFTVPPVQAEAMYSPNI
                     RYTGKLENPFVVKMRQ"
BASE COUNT          573 a          651 c          698 g          449 t
ORIGIN      
        1 cggtggacga aggaatatta cagctcatag accagaagac cgccgacccg ttcacgttct
       61 tttacagaaa gctcgcactc gaagtaaatt cctacgatac gttctcgctc ctccttccgg
      121 acgtcacgct cggcctctcc cctccgggcg gaggtttcgg ggccaggaaa gaaatgcagt
      181 tcctgagcac gcaggggata aggagggtca agcctgtcgc atactggtcg ggagttctca
      241 ccgcagacgc tgagggaaaa gcctcgttca aattcgatat gcctcagttc cagggtgccg
      301 tccgtataat ggccgtagct gtgaggggtg aggagttcgg atcgtccgaa aagttcacgc
      361 gcgtcaaatc cccgatagtg atgctgccga cggcgcccag gttcttttcc ctgaacgaga
      421 gcgttatcat cccggtctct ctcaggaacg acacgaagaa ggacgggcag ttcacgacga
      481 acatgaccgt aagcggaccc ggcacggtcg ctccaatgaa tagcggcgat cagaaagaaa
      541 agcctgccga aggagcgaag gaagcgaaaa tagattccga tatacccgac ggcagcgaga
      601 agacgattta cttcgtggtc gatacggctg acgagacagg cgctataacc ctcaatatca
      661 cggcttcggg gaacggcgag tcaacgagct tcgatatgga tgtccctgtg ctcgctgacc
      721 tccccgcgag cagaacggaa tccgtaggaa gcctcaagac cgccgacacc gagctcgcga
      781 tcgagaattt ggagtcatat cgtaccggga cgatacatag ggagctctac gtaagcaagc
      841 tgcctcttat tcagttctcg gggaagctcg aatatctgct ccaatatccg tacggctgtc
      901 tcgagcaggt cacctcccgc gtattcccgc tcatatacct ttccgatatc gcgaaggaga
      961 tcgaccccga tgtgttcaaa aaagcagacc ccgctgcgat ggtgcaggag gggataaggc
     1021 agattggcac aatgcaggtg cagggcggtg gattctccct atggccgggc ggagtggagc
     1081 ccgagccctg ggtgagcatt tatgcggcgc atttcctcgt cgaagcccgg agggccggtt
     1141 atttcgccga tgattccctt tattcgggtg ctatcgaata tttgaagagc gtcgcgaagg
     1201 ccaagcccga ttacagcaca ggagagctcg agagagccgt atatgcgctc tatgtcctga
     1261 gccgcgacgg aaagcctgac ctcagcacta tggatttcat aagggaccgc cacataggcg
     1321 agctcacagt cgagtcgaaa gcgctcctgg gcggggccta tgccgcggca ggcaacgcgg
     1381 cggccctcga agagcttctc aagggtctta acgacgtaga agaggtcacg agagaaaccg
     1441 gaggcaactt taattccacg atcaggaacc gcgccatact gctcctcgcg atcctcgacg
     1501 tcaaccctga cgacccgagg gtcccggcgc tcgtcgagcg gctgtcgaga gacgtgcaat
     1561 tgaacgcgtg gtggaccacg caggaaagct cgttcgccct cctggcaata gggcagttcg
     1621 tcaagaagca gagcgaaacc ccgccctaca ccggaaaggt gtacctgggg gacgaagagc
     1681 ttgcgacatt cagcagcgaa gcgacgttga gccttcccga cgtcacgggc gacaagccgc
     1741 tcagcataaa gatggacgac gggttcaagg aagggagcgc atactatact ctcctgacga
     1801 gcggcatccc aactaggaaa gggtttaaac ctgtcgccga gggcctcaac gttgtgaggg
     1861 aattcctgac gagggacggc gcgccgctag accccggtaa tatcgttcag ggcgacttgg
     1921 tcgtcgttag gacgaagtta cagagcgggt ccggaaagct agagaacgtc gtgattgaga
     1981 acctcctgcc ttcggggttc gaggttgaaa acccgagact caagacgacc gagacattat
     2041 cgtgggctac gggggacagc gtcgagcccg agtacctcga catcagggac gaccgcatac
     2101 tgatcttcac ggacctcccc gacgccaaat ggtacaacta ctacgcgctt ctccgtgtgg
     2161 tgaacccggg cactttcacc gtcccgcccg ttcaggcgga agcgatgtac tcgcccaata
     2221 tcaggtacac cggaaagctc gagaacccgt tcgtcgttaa aatgaggcag tgataactct
     2281 gtgcgcggga tgatgggtag cagtgaaggg atacggaata acatgaacgg tcgcggcacg
     2341 ttcttaaaaa aactggcatt gatatttttt c
//
LOCUS       DASSJH010000274         3858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_499259, whole genome shotgun sequence.
ACCESSION   DASSJH010000274 DASSJH010000000
VERSION     DASSJH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499259"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..710)
                     /gene="ybgF"
                     /locus_tag="VFJ67_05905"
     CDS             complement(21..710)
                     /gene="ybgF"
                     /locus_tag="VFJ67_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET7289956.1"
                     /translation="MSLGCVATQGDVSSVYARQTRLEAKMERLSNQVQTISQSETTTT
                     GTDVELREKVYQLEANMKELSQAYAKLESKVNQLTLGSAPPVTESGSQMQTPPETASV
                     NTEEVIFNEGYTELSDGNYDGSRAKFKEFLSKYPKSPKANDATFWIAESYYREGQFEE
                     SILEYQKFIDSYPKDQRVPLAYLKQGMSLVEIGKQEEAKLFFQTLIDKYPNSDEAKTA
                     KEKISELAVER"
     gene            complement(765..1463)
                     /locus_tag="VFJ67_05910"
     CDS             complement(765..1463)
                     /locus_tag="VFJ67_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7289957.1"
                     /translation="MERLWLRALCVCFVFTLLIAVGCGSNLDKEMADAEAALQAAKDA
                     GAEGLPEYQAAEELIEKAKEMMAAGNKDAARQLLEEARFKAIEAEGKAKSQAYAQTAD
                     EGNLQDLSPAGSQYGMVDIFFDYDQSAIRIDAKPVLDQNASIIRNSGDKFQVVVVEGY
                     CDIRGTEEYNLALGQRRAESVTNYLVGLGISPSKVQPISKGETEQFAAGTSEYDYQQN
                     RRAHFVPAVGSPSF"
     gene            1642..2430
                     /locus_tag="VFJ67_05915"
     CDS             1642..2430
                     /locus_tag="VFJ67_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET7289958.1"
                     /translation="MANHFKLSAFLKYFLIVQVSLVLFNVDSGHTGPLGDDETVLPLT
                     PLNDSVFVDVSQFGKSDITLEKESDRKSIADFQDFLQNTRNVYFSIWLIRIAQVNMLD
                     INKKEEQIIINPLRWWKNFLGFQNDGNRRGDFEWKDGDRFKTNWVAHPAFGAYTYLYY
                     RAKGYDRFTSAFGSVVQSTLFEYTIEGMIQSPSIHDLIITPGIGVPAGIVLEETSNLL
                     LSSNSGFLNALGYVINPMRIIVPDKENVNLSPLMSGQVVIGFQW"
     gene            2508..3092
                     /locus_tag="VFJ67_05920"
     CDS             2508..3092
                     /locus_tag="VFJ67_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7289959.1"
                     /translation="MSKEEGQAVAGAFWSLSPVVAITSSWQGKPNGQIAVTSVTSSIV
                     HSIPRLLVGIWKGNYTHGFIMNSGAFTIHLLRPDQLGLVRDFGFYTGRERNKFESVAY
                     RTGVTGSPVLTDAHSYAECRVLNAMDGGDMTAFLVSVVDGGILSRGEWMTLDRFYSDA
                     PYEWIEEYGEKLSRSVAYSLNIIHNIDYSPWKPR"
     gene            complement(3159..>3858)
                     /locus_tag="VFJ67_05925"
     CDS             complement(3159..>3858)
                     /locus_tag="VFJ67_05925"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7289960.1"
                     /translation="GLLGSTYYMEHPAVAAENTVAMINMDMIGGLKDNRLNVLGVGSA
                     KEWRGLIDKANSDVGLDLKYMDQAFGPSDQTVFFAEDIPAVLFFTGLHKAYHTPDDDW
                     QKINYEGEARLLEIVGNLVRELGDGENGITFSGGNPAYDGPPGISVYLGTIPEYTGGE
                     NGVRLAGVKKGSPAERSGLKENDTIIELDGIKIRNVYDYRNALAVSEAGVGVDLVIMR
                     AGKRITVNVVPEPR"
BASE COUNT          937 a         1039 c          882 g         1000 t
ORIGIN      
        1 agtacataat aataaacagt ttaacgctca actgcaagtt ccgaaatttt ttcttttgcc
       61 gtcttagctt catcagaatt cggatactta tctatcagcg tctggaagaa caatttggcc
      121 tcctcctgct tgccgatttc gaccagagac atgccctgtt tcaggtatgc gaggggcact
      181 ctttggtcct ttggatagga atctataaac ttctgatatt cgagtataga ctcctcgaat
      241 tgcccttccc tgtaatagga ttcggcaatc cagaacgtag catcgttagc tttcggtgat
      301 ttagggtact tggacagaaa ttccttgaat ttcgccctgg atccgtcgta attaccgtca
      361 ctcagctccg tatacccttc attgaatata acctcctcgg tgtttaccga ggccgtttcc
      421 ggaggcgttt gcatctggga tccggattcc gtaaccggcg gagcggagcc cagtgttagc
      481 tgatttacct tggactccag cttagcgtag gcctggctca gctctttcat gttggcttcg
      541 agctgataga ccttctctct aagctctacg tccgttcccg ttgttgtagt ctcactctgg
      601 cttatggtct ggacctgatt cgagagcctt tccatcttgg cctcgagcct tgtctgacgg
      661 gcgtaaacgc tgctgacatc gccttgagta gccacacaac cgagagacat aatagaaatc
      721 accgccacga taatattaat agcttttgca tgcataggtc agacctaaaa actcggagaa
      781 ccaaccgccg gtacaaagtg cgcccttctg ttctgctgat aatcatactc acttgttcct
      841 gccgcgaact gttcggtttc tcctttgctt ataggctgca cctttgaagg cgaaattccg
      901 agcccaacca aataattcgt tacggattcg gctctcctct ggcccagggc aaggttgtat
      961 tcttccgtgc cccttatatc gcaataccct tcaaccacaa ccacttgaaa cttatctccg
     1021 ctattcctaa ttatactcgc attctggtca agcacgggct tcgcatctat tcttattgcc
     1081 gattgatcgt aatcgaaaaa tatatcgacc atgccgtatt gggatcctgc tgggcttaag
     1141 tcttgcagat taccttcgtc ggcagtctga gcataagcct ggcttttagc tttcccttcg
     1201 gcttcgatcg ccttgaatct ggcttcttcc aacagctggc gtgcggcgtc cttgtttcct
     1261 gcggccatca tctctttggc tttttcaatc agttcttctg cggcctggta ctcgggcagg
     1321 ccttctgcgc ccgcatcctt cgccgcctga agcgcggctt cggcatcggc catctcttta
     1381 tccaaattcg acccgcaacc aactgctatc aataaagtaa acacaaagca tacacataaa
     1441 gcacgtaacc acagcctttc cattaagctc ctcctgatcg aaatgaatct tctacctgag
     1501 ataattacat tattttagct gccaagtcaa gagattttaa tcaaaaatgc gttaaatcac
     1561 aaataaaaat gaagaatttt taaaccaaat aatcgctatc aaaaatcccg tgccggtgtt
     1621 attatttaga aagaggaaat tatggcgaat cacttcaaat tatctgcatt tttgaagtat
     1681 tttttaattg tccaggtctc cctcgtattg ttcaatgtcg attccggcca tacgggaccc
     1741 cttggggacg atgagactgt attaccacta acccccctca acgactcagt atttgtagac
     1801 gtttcgcagt tcgggaaatc ggacattacc ctggaaaaag aatccgacag aaaatccatc
     1861 gccgacttcc aggattttct gcaaaacact cgtaatgttt atttctcgat ctggctcata
     1921 cgcatagcgc aggtaaacat gctcgatata aacaaaaaag aggagcagat cataataaac
     1981 ccgctcaggt ggtggaagaa cttcctcggc ttccagaacg acggaaatag aaggggtgat
     2041 ttcgagtgga aggacggaga cagattcaaa acgaactggg tcgcgcaccc ggcattcggg
     2101 gcctacacct acctgtatta cagggctaag ggctatgacc gtttcacttc agccttcggc
     2161 tcagtagtcc agagcacact gttcgagtac acgatagagg gaatgataca atctccctcg
     2221 attcacgatc tcataatcac acccggaatc ggtgttcccg cgggcatagt tctcgaagaa
     2281 acttccaacc tgcttttgtc cagcaacagc gggtttttga acgcgctcgg gtacgtgatc
     2341 aaccctatga gaataatagt ccccgacaag gaaaacgtga atttaagtcc gctcatgagc
     2401 gggcaggtcg ttataggatt ccaatggtag aagcagtttc acgtccgttg caaaagtgaa
     2461 accaacccgt ttgtcacaat agaatcggat attcaggacg acaatcaatg agtaaagaag
     2521 aaggacaagc ggtcgctgga gcgttctggt ctctttcccc ggtagttgca attacaagtt
     2581 cctggcaggg caagccgaac ggacagatcg cggtaacgtc cgttacctct tccatagtgc
     2641 attccatacc gagactcctt gtcggtatat ggaaggggaa ttatacgcac gggttcatca
     2701 tgaacagcgg ggcgttcacg attcacctgc tgaggcccga ccagctcggt ctcgtgaggg
     2761 acttcggatt ctacaccgga agggagagaa acaaattcga gagcgtcgcg tacaggacag
     2821 gcgttacggg cagccccgtg cttacggacg cacattccta cgccgaatgc agggtgctta
     2881 acgcgatgga cggaggcgac atgacggcct tcctcgtaag cgtcgtagac ggcggaattc
     2941 tcagcagggg cgagtggatg acactcgacc ggttttactc tgacgctcct tacgaatgga
     3001 tagaggagta cggagagaag ctctccaggt ccgtagcgta ttcgttaaac ataatccaca
     3061 atatagatta ttcaccgtgg aaacctcggt aacattggag acggcggaca tgaagcccgg
     3121 cctgccgata gctaggcgtc atatcgatga gaaccgtctc agcgcggctc gggcacgacg
     3181 ttcacggtaa tccgcttccc cgccctcatt atcacgagat cgaccccaac accggcttca
     3241 gaaaccgcga gcgcgttccg gtaatcatat acgttccgaa tctttattcc gtcgagctct
     3301 ataatcgtgt cgttctcctt cagtcccgat ctctcggccg gacttccctt tttcacaccc
     3361 gcgagcctga ccccgttttc accgcctgta tactccggga tggtccccag atagacgctg
     3421 attccggggg gtccgtcgta agccggattc ccgccgctga acgttattcc gttctcaccg
     3481 tcacccagct ccctaacgag attccccacg atctcgagga gccgggcctc gccctcgtag
     3541 ttaattttct gccaatcgtc atcaggtgtg tgataggcct tatgaagtcc ggtaaagaag
     3601 agaacggccg gtatatcctc ggcgaaaaaa acggtctggt cgctcggccc gaacgcctga
     3661 tccatgtatt tcaaatccag accgacatcc gaattggctt tatctatgag gcccctccac
     3721 tccttggccg agccgacccc cagtacgttg agcctgttgt ccttgagtcc cccgatcata
     3781 tccatattta tcatcgctac ggtattttct gccgcgacag ccggatgctc catataataa
     3841 gtagagccca ggaggcct
//
LOCUS       DASSJH010000275         4491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1364173, whole genome shotgun sequence.
ACCESSION   DASSJH010000275 DASSJH010000000
VERSION     DASSJH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364173"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..2473)
                     /gene="priA"
                     /locus_tag="VFJ67_05930"
     CDS             complement(59..2473)
                     /gene="priA"
                     /locus_tag="VFJ67_05930"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET7289961.1"
                     /translation="MNGTIQVAVPMPGEALYTYSVPPHLKEGTMLGKRVLVPFRNRRT
                     IGFIVGPGEPPPDIELKDILDIIDDEPLFDEKRLAFLKWAADYYMTSLGIVLKAAHPG
                     GLGASIRRVIKITGEGIGALEKGRLTGHEALVLKTVLNAGEISSHKLLSLVDNTSAEL
                     LNSLRRRGLVEFSYELKSDPKVKTEKIISASPGAAVSDPLLKRKKTKSHILEYLIENG
                     RVPLDDLKEVFGNVGAHLSWLAENAFIEIGEEEIARDPFAGIEIKGDVKPALTLDQEI
                     ACGKIKDAVGKGRFAPFLLHGVTGSGKTEVYLRAIEEVIGKGREAILLVPEISLTPLL
                     VKRVRARFGRNVAVIHSALSDGERFDSWRMASRGEVKVVIGARSAIFAPFRNIGIIIV
                     DEEHETSYKQDETPGYNARDLALVLGRMTDSVVVLGSATPSAESYYNALKRRFEYLSL
                     PLRVEDRPLPTVEIADMKYEGGAIFSQRLREEIMDNFWHDKQTILFLNRRGYATLIIC
                     QACGEIMKCPNCSVSLTYHAKEDSVRCHMCGLSEKLTGACPKCGESLRGLGIGTQKVE
                     EELRRIIPEAKAARMDRDTVAGKKSLLALYGKLERGEIDVLIGTQMVAKGHDLPGVTL
                     VGVISADMALGIPDFRSGERTFQLVTQVAGRAGRGDTPGKVIVQTYNPEHPSIEHAVR
                     QDSRGFLDEELKLREELGYPPYSRLVNVRFTGRIERETRDASERAGETARKLVTKLKP
                     GALQILGPSPCPVSKIRNRYRFQMLLKSTNAGLLRSFTKKLRKLMSNVTGDVRVSVDV
                     DPYNLS"
     gene            2833..3690
                     /locus_tag="VFJ67_05935"
     CDS             2833..3690
                     /locus_tag="VFJ67_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7289962.1"
                     /translation="MGRLDRLLNKADDYKEAGRFEEALKELAKAVKIAPRDPDVYLSY
                     ALTYDAMEDFQSSVSYFRKAVELNPEDPYIWTQLGITLSRMGRQGESLRAYNAALDLD
                     SEYVIAKWHMALTYRSIGLYEDALRYFTECRESDADIDYIKDEIGYQTGLCYFDMGWT
                     KEALYEFRKHLEACPGDTWAHMSVGNCYFDFGWIDESAAKFKEVIDLSPEFIPAYNAL
                     ALSLAEKGWYDEALDVLRTALKISPDDETVKDNMDYIQSLKEDDDGAKGVILLSLILQ
                     LLKRKEISD"
     gene            complement(3705..>4491)
                     /locus_tag="VFJ67_05940"
     CDS             complement(3705..>4491)
                     /locus_tag="VFJ67_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7289963.1"
                     /translation="IHIDVDAVLLGELAFAPFDRLFVHGRDLGPVSMYVMGVILLLNL
                     AFILVFYKELKLATFDPGLAAALGFAPGLIHYGLMGLVSVTAVGAFNAVGSVLVVALM
                     ITPPAAAYLLTDSLPRMIVLSALIGILSAVSGFWAAILIDANIAGSIATVSGLIFLLV
                     FLLAPERGLLAIAVRRSRQKREFAGRVLAIHLLNHEGTPEESRESHIAHVEETLRWKE
                     GFASSIVGYSVKSGIIFVENGHLRLTDKGREFAGRSVVEEFIV"
BASE COUNT          938 a         1364 c         1189 g         1000 t
ORIGIN      
        1 atgtagcatg agtccaaata agatagggag gatttgtttt ttcctaggtt cccccacttc
       61 aactaagatt atacggatcg acgtctacgg aaaccctcac gtctccggtg acgttcgaca
      121 tgagcttcct cagtttttta gtgaaactgc ggagtagccc cgcgttcgtg gatttaagaa
      181 gcatctgaaa cctgtacctg ttccttatct tgctcacggg gcacggcgaa ggcccgagaa
      241 tttgcagcgc gcccggcttc agctttgtga cgagcttcct cgcggtctct cccgccctct
      301 ccgatgcatc acgggtctca cgctctatcc ttcccgtgaa cctcacgttc acgagcctcg
      361 agtaaggcgg atagccgagc tcctctctca gcttcagctc ctcgtcgagg aaccccctgc
      421 tgtcctgcct cacggcgtgc tctatgctcg gatgctccgg attataagtc tggactatca
      481 ccttccccgg cgtgtcccca cgccctgccc ttccggccac ctgagtgacc agctggaacg
      541 tcctttcgcc tgagcggaag tcggggatac cgagcgccat gtccgccgag atgacgccca
      601 cgagcgttac accagggagg tcgtgcccct tggctaccat ctgtgtcccg atgagcacgt
      661 ctatctcccc gcgctcgagc ttcccgtata gagcgagcag gctcttcttt cccgcgaccg
      721 tatccctgtc catgcgcgcc gccttggctt cggggattat ccgcctgagc tcctcctcga
      781 ccttctgcgt cccgataccg agcccgcgca gggactcacc gcatttcggg cacgcgcccg
      841 tgagcttctc cgagagaccg cacatgtggc acctcaccga gtcctccttc gcgtgatagg
      901 taagggaaac ggaacagttg gggcacttca ttatctcccc gcacgcctgg catatgatga
      961 gcgtcgcata gccgcgcctg ttgagaaaga gtatcgtctg cttatcgtgc cagaagttgt
     1021 ccattatctc ctcacggagc ctttgcgaaa atatagctcc gccctcgtac ttcatgtcgg
     1081 cgatctctac cgtgggcagg ggcctgtcct cgaccctgag cggcagggaa aggtattcga
     1141 acctgcgctt gagggcgttg tagtatgatt ccgcggacgg cgtagccgat cccagcacaa
     1201 cgaccgaatc agtcatcctt ccgagcacga gcgcaaggtc ccttgcgttg tagccgggcg
     1261 tctcgtcctg tttgtaagac gtctcgtgct cctcgtcgac gattattatg ccgatgttcc
     1321 tgaagggcgc gaatatcgcc gagcgagccc ctatcacaac cttcacctcg cctctgctcg
     1381 ccatgcgcca ggaatcgaac ctctccccgt ctgacagcgc gctgtgtatg acggcgacgt
     1441 ttcttccgaa cctcgccctc accctcttta cgaggagcgg cgtgagcgat atttcgggca
     1501 cgaggagtat ggcttccctc cccttcccta tgacctcctc tatcgcgcgc agatagacct
     1561 ccgttttccc gctccccgtg acgccgtgga gcaggaacgg cgcgaaccgg cccttcccga
     1621 cggcgtcctt tatcttcccg cacgcgatct cctggtcgag cgtgagggcg ggttttacat
     1681 ccccctttat ttcgatgccc gcgaatgggt ccctcgctat ctcttcctct ccgatttcga
     1741 tgaaggcgtt ctccgcgagc catgagaggt gtgcccccac gttcccgaac acctctttga
     1801 gatcgtctag aggcactctg ccgttctcga tgaggtattc gagtatatgc gacttcgtct
     1861 tcttcctttt aaggagcgga tcgctcacgg cggcgcccgg agaggccgat attatcttct
     1921 cggtctttac cttcgggtcc gatttcagct cgtaggagaa ctctacgagc cctcttctcc
     1981 tgagcgaatt gaggagctcg gccgacgtgt tgtcgacgag agagaggagc ttatgcgagc
     2041 ttatctcccc cgcattgagc acggtcttga gcacgagggc ttcatgcccc gtgagccttc
     2101 ccttttcgag cgcgccgatt ccctctccgg taatttttat cacgcgcctt atgctcgcgc
     2161 cgagtccgcc cggatgagcg gccttgagaa cgatgccgag cgaggtcatg tagtagtcgg
     2221 cggcccactt caggaacgcg agcctcttct cgtcgaagag cggctcgtcg tcgattatat
     2281 ccaggatgtc tttaagctcg atatcgggag gaggctcgcc cggccccacg atgaacccta
     2341 tcgtcctcct gttcctgaaa ggcacgagga cgcgctttcc gagcattgtc ccctctttca
     2401 gatgcggagg gacgctgtac gtatagagtg cctcccccgg catggggacg gcgacctgaa
     2461 tggttccatt catttacacg ggagtctaat tgttttcccg attgcgtgca atagaagccg
     2521 ggctccggcc gacgcatatg cctgattgca gatacaaatc aaatgccgcg gctaagcccg
     2581 cgaaaggatt tatgaacgta actttgtgag gactcacccg gccacgcaca ccgccgcatc
     2641 cgaggtttct aatatcttat ctgaggatac gcctctaact cacaattctc attgatgaat
     2701 atattatgca cagtttgtcc acaggtcctc cccggagtta aaatcaataa attaagtttc
     2761 agatgttgac aacttcaatc acagcaaata tgatttaaac aggtcggtac caaaggccgg
     2821 ccggaacgtt caatgggaag gttggacagg ctcctaaaca aggcagacga ttataaggaa
     2881 gcgggccgct tcgaggaagc tctgaaagag cttgccaagg cggtcaagat agctccccga
     2941 gacccggacg tttatctttc ctacgccctc acctacgacg cgatggagga ttttcaatcc
     3001 tcagtctcat atttcagaaa ggccgtcgag ctcaacccgg aagaccccta catctggaca
     3061 cagctcggta taaccctctc gaggatggga aggcaggggg agtcgctccg cgcttacaac
     3121 gcagccctcg atctcgattc cgaatacgta atcgcgaagt ggcacatggc gctgacctac
     3181 aggtcgatcg gcctctacga agacgctctc aggtacttca cggagtgcag ggaatcggac
     3241 gccgatatcg attacattaa agacgagatc ggctatcaga ccggcctctg ctacttcgac
     3301 atggggtgga ccaaggaggc cctctacgag ttcaggaaac acctcgaagc ctgcccgggg
     3361 gacacgtggg ctcacatgtc cgtagggaac tgctacttcg acttcggatg gattgacgaa
     3421 tccgccgcga agttcaaaga ggttatcgat ctctcgcccg agttcatacc ggcttataac
     3481 gcgctcgctc tttccctcgc ggaaaagggc tggtacgacg aagcgctcga cgtgttaagg
     3541 acggcgctca agatatcgcc cgacgacgaa accgtaaaag acaacatgga ttacattcag
     3601 tcgcttaagg aagacgacga cggcgccaag ggcgtcatac tcctgagcct gatcctccag
     3661 ctcctgaaga gaaaagagat atccgactga gcatttgtta aggctcagac tatgaattcc
     3721 tcgacgaccg acctgcccgc gaactccctc cccttatctg tcagcctgag atgcccgttc
     3781 tcgacaaaaa ttattccgct tttcacggaa taaccgacta tgcttgatgc aaatccctct
     3841 ttccacctga gcgtctcctc aacatgcgct atatgcgatt cacgcgactc ttcgggagtc
     3901 ccctcgtgat tgagcaggtg tatggcgagc accctgcccg cgaactcgcg cttctgtctc
     3961 gaccgtctca cagctatcgc cagtagccct cgctcagggg ctaagaggaa taccaggagg
     4021 aatatcaggc ccgaaacggt tgcaatcgag cccgcgatgt tcgcgtctat gagtatggcg
     4081 gcccagaacc cggagaccgc actcagaatc ccgataagtg cgcttagcac tatcatgcgc
     4141 gggagactgt ccgtgaggag gtaggccgcc gcgggaggcg ttatcatgag agcgaccacc
     4201 agcaccgagc cgacagcgtt gaacgctccg acggccgtaa ccgagacgag gcccatgagg
     4261 ccgtagtgta taagcccggg cgcgaaaccg agcgcggcgg ccaggccggg gtcgaatgtg
     4321 gccaatttca gctctttata aaaaacgagt atgaatgcaa ggttcagtaa aagtattacg
     4381 cccatcacat acatagacac gggtcccagg tcgcggccgt gtacgaacag gcggtcgaac
     4441 ggggcgaacg ccagttcccc gaggagcacg gcgtctacgt cgatgtgtat a
//
LOCUS       DASSJH010000276         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_282701, whole genome shotgun sequence.
ACCESSION   DASSJH010000276 DASSJH010000000
VERSION     DASSJH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282701"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFJ67_05945"
     CDS             complement(<1..394)
                     /locus_tag="VFJ67_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289964.1"
                     /translation="MKNIPLALLCLFPLLAGCIDVGGSSSNPIQGEVVERRALTEEEV
                     AIWNEQAECIETASKSSPDIEDLNATDVPVVPEITIRKDLMCGNIPPDNLIACQSEDE
                     IAILDGLDQETFNRALRHEEIHYILFFET"
     gene            636..1169
                     /locus_tag="VFJ67_05950"
     CDS             636..1169
                     /locus_tag="VFJ67_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289965.1"
                     /translation="MKRVSVTTSYVVSFLFFLTVFFAASVLTPFTDEARAAGGTAKGT
                     IKLKDKTVDVKYAYFIKAPDSFDESKTVSTVIITPNDISQKITECQSGSCASGITEGM
                     TVGKEDFGGTIRVVYWVVTKDGMMQYSGNDDVSALVLTTDTADRMAGKLTIDDSAAEG
                     PKIDVEFDAPLAKEFKE"
     gene            1322..2125
                     /locus_tag="VFJ67_05955"
     CDS             1322..2125
                     /locus_tag="VFJ67_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289966.1"
                     /translation="MSNMAESLNKYIFPLLALIAFAIFHYGPSGVSDAEAVAYNNKEK
                     IYLKDITPILYELSEVGKSVSQKAVSLQNASPEECSYEFGYYQGIVEGLRVRLNTIPP
                     PPRMITIQSTALLAIGDYISGLSLYTRACTEDDYNMKAKLGYDGRQRLIDADIKIRQV
                     NYLITNPQVQARAQSQTQTQTPAAPDNKIAQMCASTWPADQRMQDYCVKNQTEALIKL
                     NQMNRKYAPGSRERQTIQNCSSLWKKGNTYDYRMVVFCVENVLGKQATQ"
BASE COUNT          566 a          560 c          608 g          447 t
ORIGIN      
        1 cggtctcgaa aaagagaatg taatggattt cctcgtgcct caaggccctg ttgaacgtct
       61 cctggtcgag tccgtcgagt atggcgattt catcttccga ctgacaggca ataaggttat
      121 cgggaggaat gttaccgcac atgaggtctt tcctgatcgt gatctcgggt acgaccggaa
      181 cgtcggttgc gttcaggtcc tctatgtcag gactcgattt gctggcggtc tcaatgcact
      241 ccgcctgctc gttccatatc gcgacctctt cctctgtgag cgctctcctc tcgacgacct
      301 cgccttggat agggttcgag gatgagccgc ctacgtcgat gcagcccgct aataacggga
      361 aaagacagag caatgccaaa ggaatgtttt tcatattccc cacctcttcg aatcaataat
      421 atacaaagcg cctttaattt ccactccggt ggaaaaatgc ctgcgttccc ggtgattacg
      481 gaggcccatt gactccgcca cggtgctcgc gcataatctc ggcttgaggg gcgaggccgt
      541 agataatcct tccatataaa gcggaaggcg attgcgcaat tcataattgc cgtcataggg
      601 gccgggtccc gaaatcgggc aaaaggagat gtgaaatgaa gagagtttcc gtaacaactt
      661 cgtatgttgt ttcattctta ttttttctca cagttttctt cgcggcgtcc gtgctgactc
      721 cgtttacgga cgaagcccgg gcagcgggag gaacggccaa agggacgata aagctcaagg
      781 acaagacggt ggatgttaaa tacgcttact tcatcaaggc cccggattcc ttcgacgaga
      841 gcaagacggt aagcacggtt ataatcacgc ccaacgatat aagccagaag atcacggaat
      901 gccagagcgg gtcgtgcgcg agcgggataa cggagggcat gacggtcggg aaagaggact
      961 tcggaggcac gatacgggtc gtttactggg tcgtcacgaa agacgggatg atgcagtatt
     1021 ccgggaacga cgacgtctcc gcgctcgtgc tcacgacgga cacggccgac aggatggcgg
     1081 gcaaactgac tatagacgac tccgccgcgg aaggcccgaa gatcgacgtc gagttcgacg
     1141 ctccgctcgc gaaggagttc aaggaatagg cacctactcc ggctgatggt attcgtttgc
     1201 ggtcggaaat cgatctgaaa aattatgctt agaagcagat tgattatcgt gtagtgtatt
     1261 ttaagacagg acgggagagc gaaagccgag cccggagaac agacaggagg tgtcataagt
     1321 aatgagtaat atggccgaat ctctcaataa atatatattt ccgctgttgg cgctgatcgc
     1381 tttcgcgata ttccactatg gcccgtccgg tgtaagcgac gcggaagccg tcgcttataa
     1441 caataaggag aagatatacc tcaaggacat cacccccata ctctatgagc tttctgaagt
     1501 tggcaaatcc gtgagccaga aggccgtaag ccttcagaac gcgagcccgg aagaatgctc
     1561 gtacgagttc gggtactacc aggggatagt cgaaggcctg agggtgagac tcaacacgat
     1621 ccccccacca ccgaggatga taaccataca gtcgaccgca ctcctcgcga taggcgacta
     1681 cataagcgga ctcagcctgt acacgagggc gtgcacggaa gacgattata atatgaaggc
     1741 caagctggga tacgacggca ggcaaaggct catcgacgcc gacataaaga taaggcaggt
     1801 gaactatctg atcacgaatc cccaggtgca ggctcgggct cagtctcaga ctcaaactca
     1861 aacaccagcc gctcccgata acaagatcgc tcagatgtgt gcctcgacgt ggcccgccga
     1921 ccagaggatg caggattact gcgtaaagaa ccagaccgag gcgctgatca agctcaacca
     1981 gatgaaccgc aagtacgcgc cgggctcgag agagaggcag acgatccaga actgcagctc
     2041 cctatggaaa aaagggaaca catacgacta ccgcatggtc gtattctgtg tcgagaacgt
     2101 actggggaaa caggcgaccc aatagcgggg ctgtccgcat cgcctgagag gtgcgcatgc
     2161 tcgaaatcat ccccttcgag g
//
LOCUS       DASSJH010000277         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_175097, whole genome shotgun sequence.
ACCESSION   DASSJH010000277 DASSJH010000000
VERSION     DASSJH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175097"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFJ67_05960"
     CDS             complement(<1..331)
                     /locus_tag="VFJ67_05960"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET7289967.1"
                     /translation="MTDLKKYIRDIPDFPKKGIIFHDITPLLQNAGAFEAAIERMAEM
                     LGGCEADYLVGIESRGFIFASALAIKLRTGLVIVRKPGKLPSVTINASYDLEYGSDSL
                     EVHRDAIH"
     gene            complement(351..1004)
                     /locus_tag="VFJ67_05965"
     CDS             complement(351..1004)
                     /locus_tag="VFJ67_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7289968.1"
                     /translation="MTLALLGACAKSPTPPPSIPRVSEKDIQKCLTGRLTVKEGYRYH
                     MLEEGETLYRISIMYGTTVEELIAVNNISDYTDIPAGTMLKIPGAVVSSGLVWPLPGK
                     ISSGFGRRGGRYHWGIDIPAPNGTPIRAAADGLVLISTDGLRGFSGYGRIVVIEHGGG
                     ISTLYAHNRRNDVKPGTCVRAGETIAEVGSTGNAIGSHLHFEVRNDGKPVNPLNYLP"
     gene            complement(973..1449)
                     /locus_tag="VFJ67_05970"
     CDS             complement(973..1449)
                     /locus_tag="VFJ67_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HET7289969.1"
                     /translation="METVIGVIGAGEVTEDEYVTALETGREIARRKFTLVCGGLGGVM
                     EAACKGAKSEGGLTVGVLPGEFRKEANPYVDITVVTGMGHGRNIIVVRSSDAIIAIGG
                     SFGTLSEIAFALRLGIPVVGIKTWDVSTDIKKAENPREAVEMAAGLVDARAARGMR"
     gene            1635..2279
                     /locus_tag="VFJ67_05975"
     CDS             1635..2279
                     /locus_tag="VFJ67_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289970.1"
                     /translation="MNIFTNSRLLILIALSLFITVRHAAAQQNPDLAPLGFAFGISDD
                     DAEDIIETNGKRIMENNVDSKKIRTIVIQGMIVTLPLDLEGKDVSTGLEFYDGKLLSS
                     SLNLNTKDSFEESQMRDEFMKYLTGKYGEPSSSDSMLSFKTWTWNMPDVKLVFHTNEK
                     KYDMKVVYTYEPVDQAKHEKELDKLRGTEESNPAEDMFINGDYSKPKYYDETGH"
     gene            complement(2320..2823)
                     /locus_tag="VFJ67_05980"
     CDS             complement(2320..2823)
                     /locus_tag="VFJ67_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289971.1"
                     /translation="MRPKIQGVSLLLLVLASASCANMGLIMQNMNPEQVSKDELKDLP
                     TEPTPKYGIRVGKDSNVNADMSEILENVDYQFTEFSECFSIKDGGEKARQYTIAVVNG
                     TFRCEYYFGSCNGEYDSDYGLIIVSYKAFSRKGILATLKHEWAHVYGLLRDDHQNLKD
                     VKWCTRY"
BASE COUNT          641 a          815 c          721 g          749 t
ORIGIN      
        1 gatggatcgc gtccctgtgc acctcgagcg aatccgatcc gtattcgagg tcgtaggacg
       61 cgtttattgt aacggacggc agcttccccg gcttcctcac tataactagt ccggtgcgga
      121 gcttaatagc cagggccgaa gcgaatatga acccgcgcga ttctataccc acgaggtaat
      181 cggcttcgca tccgccgagc atctccgcca tcctttcgat cgcggcttcg aaagcgcctg
      241 cattctggag gagaggggtg atgtcgtgga atattatgcc cttcttcggg aagtccggta
      301 tgtccctgat gtatttcttg aggtcggtca tttagaattc tcctatcgtg tcatggcaga
      361 taattaagcg ggtttaccgg cttgccgtcg ttcctcacct cgaagtggag gtggctgccg
      421 atcgcgttcc ccgtagagcc tacctcagcg atcgtctctc ccgctctgac gcatgtcccc
      481 ggtttcacgt cgttccttct gttgtgtgca tacagtgtgc ttatcccccc gccgtgctca
      541 atcacgacga tcctgccgta tccggaaaac cccctcagtc cgtcagtgct tataagcacg
      601 agcccgtccg cggccgccct gatcggcgtt ccgttcggag ccgggatgtc tatgccccag
      661 tggtaccttc ccccgcgcct gccgaaaccc gaggatatct ttcccggaag gggccagacg
      721 agccctgaag aaacgaccgc tcccggtatc ttgagcatgg tgcccgcggg gatgtccgta
      781 tagtcgctta tattattcac tgcgatcaat tcttcgaccg tcgttccgta cattatggat
      841 atcctgtaaa gggtctctcc ctcttcgagc atgtggtatc tgtacccttc ctttacggtg
      901 agccttccgg tcaggcactt ctgtatgtcc ttctcgctca ccctgggtat cgacggcgga
      961 ggggtagggc tcttagcgca tgccccgagc agcgcgagcg tcaacaagcc cggcagccat
     1021 ttccaccgct tccctgggat tctctgcctt tttaatatcg gttgatacat cccaagtttt
     1081 tatccctacg accggaattc ccaatctgag agcgaaggcg atttcagata atgtaccgaa
     1141 gctccctcct attgcaatta tagcgtcgct cgagcggacg actatgatgt tcctgccgtg
     1201 gcccatgccg gtgacgacgg ttatatcgac gtaggggttg gcctccttcc tgaattcacc
     1261 cgggagcacg cccacagtca ggcctccctc tgatttcgct cccttgcatg cggcttccat
     1321 aacgccgccc agtccgccgc atacgagcgt gaatttcctc cgcgctatct ccctgccggt
     1381 ctcaagcgcc gtaacgtact catcctccgt tacctcgcct gctcctatca ctccgataac
     1441 ggtctccata tccggcaaga ttatatgctt tttcaagccc ttacacaaac gggataacgg
     1501 cttcgaaccg tattaccccg ctgcgggcgc cccgcggaaa ggattaataa aacttgattt
     1561 tgacacggtc atcttatacc tttttatgat atttgagccc agagctttcg ctaaatgtaa
     1621 acagggggga ataaatgaat atttttacaa acagccgttt attgatactg atcgctcttt
     1681 cgttatttat tacggttcgt catgccgccg ctcagcagaa ccccgacctt gctccgctcg
     1741 gtttcgcgtt cggcatctcg gacgacgatg ccgaggatat aatagagaca aacgggaaaa
     1801 ggataatgga aaacaacgtc gactcgaaga aaataaggac tatcgttata cagggcatga
     1861 tagtcacact gcctttggac ctcgagggaa aggacgtgag caccgggctc gaattctacg
     1921 acggtaaact tctttcgtcc tctctgaatt tgaatacgaa ggactcgttc gaggagtcgc
     1981 agatgcgcga cgagttcatg aagtatctca cgggtaaata cggcgagcct tcgagctccg
     2041 acagcatgct ctccttcaag acatggacgt ggaatatgcc cgacgtaaag ctggtatttc
     2101 atacgaatga aaagaagtac gatatgaagg tcgtttatac gtatgagccg gttgaccagg
     2161 cgaaacacga gaaggagctc gacaagctgc gtggcactga ggagtcgaac cctgccgagg
     2221 atatgtttat caacggggat tacagcaaac ccaagtatta cgatgaaact gggcactgaa
     2281 atctgtcggg attccgcggc gtcgcggcga gaagccggat caatacctgg tacaccactt
     2341 aacgtctttc aaattctgat ggtcgtccct caataacccg tacacatgcg cccattcgtg
     2401 tttgagggtg gccaatatgc cttttctgct gaacgctttg tagctaacta ttattaatcc
     2461 gtaatcagag tcgtactccc cattgcagct cccgaaatag tactcgcatc tgaaagtgcc
     2521 gttcactacg gcgatcgtat actgcctcgc cttctctccg ccgtccttaa tgctgaagca
     2581 ttcgctgaat tcggtgaatt gataatccac gttctcgagg atttcgctca tatccgcgtt
     2641 tacgttcgaa tccttaccga ccctgatgcc gtatttcggt gtcggttccg tggggagatc
     2701 cttcaattcg tccttgctaa cctgttcggg gttcatgttc tgcatgatga gccccatatt
     2761 ggcgcacgag gctgacgcaa gcaccaggag aaggagcgaa acgccctgta tcttcggtct
     2821 cataatccga tacatactat aactcaatgc gggccgattg gtgatgggta ttcggcttcg
     2881 acataacgta taaaatgttt tatgcttggt aaaacacgga aagtgt
//
LOCUS       DASSJH010000278         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_926286, whole genome shotgun sequence.
ACCESSION   DASSJH010000278 DASSJH010000000
VERSION     DASSJH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926286"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /gene="uvrA"
                     /locus_tag="VFJ67_05985"
     CDS             <1..1658
                     /gene="uvrA"
                     /locus_tag="VFJ67_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7289972.1"
                     /translation="MHVRVFLSKYRSAFTCDGCIGSRLVKEALWVKVGGMNIYELSLL
                     PIDILREFFEGLELSDFESSIAHEILKQIKSRLDFLVKVGLEYLTLARLSRTLSGGEA
                     QRVNLACQLGATLTETLYIMDEPSIGLHPRDVDRLISLIKELRDRDNTVVVVEHDFEM
                     IKSADNIIELGPLAGERGGDIVYQGEVKKLLNGSADSLTKRYLTGKTQILTPSKRRKG
                     SGKSLTVTGARENNLKNLTVSFPLRTFICVTGVSGSGKSSLVNDVIYSALARKFRGEV
                     ERVGKYGEIRGAGNLSDVIMLDQNPIGKSSRSNPVTYIKAYDEIRKTMAGTWQAKSKG
                     LTPSHFSFNVPGGRCETCEGEGVQQIEMHFLADVFVTCEECGGKRFNKDVLSVRYRER
                     NIDEILSLTVNEAINFFYEVPVIGRKLRILQDVGLGYIRLGQAAPTLSGGEAQRIKIA
                     RELGRKGGKDILYILDEPTVGLHAEDVKKLLEVINKLVLAGNTVLVVEHNLDVIKSAD
                     YILDLGPEGGEEGGFVIAQGTPEDVAASDESHTGRYLKKAMSS"
     gene            1849..>2478
                     /locus_tag="VFJ67_05990"
     CDS             1849..>2478
                     /locus_tag="VFJ67_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET7289973.1"
                     /translation="MSIGKRDYYEVLGVERGAGQQEIKKAYRKLAMQYHPDHNDAPDA
                     EEKFKELSEAYGVLSDAEKRARYDRGGFSALDGMSAEDIFSGINFGDIFGGGGLGGSI
                     FDEIFGFGRRRGPARGRDIEVEITVPLKRILTGGEETVSYGRRVACPDCGGSGAKAGT
                     EPKTCTECGGSGQKVYTSRQQGMTFQQVAVCPSCKGRGSIIEEFCPACAG"
BASE COUNT          618 a          595 c          783 g          482 t
ORIGIN      
        1 agatgcatgt gagggtattc ctcagcaagt acaggtcggc gttcacgtgc gatggctgca
       61 tcgggtcaag actcgtgaag gaggcccttt gggtgaaggt aggcgggatg aacatatacg
      121 agctttccct gctgcctata gacatactca gggaattctt cgagggtctc gagctcagcg
      181 atttcgaaag cagcatagcc cacgagatac tgaagcagat taaatcgaga ctggatttcc
      241 tcgtaaaagt cggtctcgaa tatctcactc tcgcaaggct gtcgaggact ctctccgggg
      301 gagaggcgca gcgggtgaac cttgcctgtc agctgggcgc aacgctgacg gagacccttt
      361 atataatgga cgagccctcg atagggcttc acccgaggga cgtcgaccgt ctcatctcgc
      421 tgataaagga gctcagggac agggacaata cggtagtagt cgtagagcac gatttcgaga
      481 tgataaagtc ggccgacaat attatcgagc tcgggccgct cgccggcgaa aggggagggg
      541 acatagtcta tcagggcgag gtgaagaagc tcctcaacgg ctcggccgat tccctgacga
      601 aaagatacct taccggcaag acgcaaatat taacaccttc aaagaggcgg aagggcagcg
      661 ggaagtccct cactgtaaca ggcgcccggg agaacaacct gaaaaacctg actgtctcgt
      721 ttccgctgag gactttcatt tgcgtaaccg gcgtctcggg ctccgggaag agctcgctcg
      781 tgaacgatgt gatttactct gccctcgcgc ggaaattcag gggcgaggtc gagagggtgg
      841 gaaagtacgg cgagatcagg ggggccggca acctgtcgga cgtaataatg ctcgatcaga
      901 accccatcgg aaagagctcg aggtcgaacc ccgttaccta cataaaggcc tacgacgaga
      961 taaggaagac aatggcaggc acctggcagg ctaaatcgaa ggggctcact ccgtcacatt
     1021 tttcgttcaa tgtccccggg gggcggtgcg agacgtgcga gggcgagggc gtgcagcaga
     1081 tagagatgca cttcctcgcg gacgtgttcg tgacgtgtga agagtgcggg ggcaaacggt
     1141 ttaacaagga cgtcctgtcc gtccggtaca gggaaagaaa tatagacgag atactttctc
     1201 tcacagtaaa cgaggccata aacttctttt acgaggtccc ggtgatcggc aggaagctga
     1261 ggatactcca ggacgtcggc ctcggataca tcaggctcgg tcaggcggcg cccacgcttt
     1321 ccggagggga ggcgcagagg ataaagatcg cgagagaact ggggaggaag ggagggaagg
     1381 acatcctcta catactcgac gaaccgactg tcggactcca tgcggaggac gtgaaaaagc
     1441 tgctcgaggt tataaacaag ctggtcctgg ccggcaatac cgtgctcgtc gtggagcata
     1501 atctcgacgt gatcaaatcg gccgattaca ttcttgacct cggcccggaa gggggcgaag
     1561 agggcgggtt tgttatcgcg caggggactc ccgaggatgt tgcggcgtcg gatgaatccc
     1621 acaccggcag atatttaaag aaagccatgt cttcctgact cattggctaa acgctgtttt
     1681 gcatacgagt attcgcttga ggataggaac cgggcttatt ttcgattaaa atacactttc
     1741 cgactcaggt ttaatccccc ataaaaaccc gcattggctg tttatgcggt cttgaaaaaa
     1801 gggaaatgat ttttatataa gaaataaaag catatacggg tttggcttat gtcgattgga
     1861 aaacgagatt attacgaggt gctgggcgtc gagagaggcg cgggccagca ggagataaaa
     1921 aaggcctacc gcaagctcgc catgcagtat caccccgacc ataacgacgc gcccgacgcc
     1981 gaggagaaat tcaaagagct ctcggaagct tacggcgttc tctcggacgc cgaaaagcgc
     2041 gcgcgctacg acaggggagg gttctccgct ttagacggca tgtccgcgga ggacatattc
     2101 agcggaatca acttcgggga catattcggc ggtggcgggc tgggcggaag catattcgac
     2161 gagatattcg gattcggcag gaggagaggg cctgcccgtg gcagggacat agaggtcgag
     2221 ataacggtgc ccctcaagcg gatactcacg gggggagaag agaccgtttc atacggcagg
     2281 agggttgcgt gccccgattg cggcgggtcg ggcgcgaagg cagggactga gccgaaaacg
     2341 tgtacggaat gcggcggatc gggccagaaa gtctatacga gccgccagca ggggatgacc
     2401 tttcagcaag tggcggtctg cccgtcctgt aagggacggg gttcgataat cgaagagttc
     2461 tgtcctgcat gtgcaggg
//
LOCUS       DASSJH010000279         4514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_602574, whole genome shotgun sequence.
ACCESSION   DASSJH010000279 DASSJH010000000
VERSION     DASSJH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602574"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFJ67_05995"
     CDS             <1..886
                     /locus_tag="VFJ67_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289974.1"
                     /translation="SLRIKDEPVDHTTRLPKMFEDEGTDDELVIMKDTDIPEADGLNS
                     DLQRTQTNERDYVRHSTESLGVDMGVLASRPTPAAGYGGGPGPSYRDQVRIYSSGSRS
                     SGGFFARAAYTLLILIVFAVIIGASYIILSNTGVIPRETADSIEMYARSLIPVSISGS
                     LGNEVVITAHSGKWLDTRNGPMYVISGTVTNESGRPVSYIKIRSEFISDGQTVYDNMV
                     YAGNTFTENELRVSPLSDTLLKLKKRSGDIDYYNTEKLAGLDSNVQPGESIPFFAVYP
                     ASGRMLGLKYNLDVVGYE"
     gene            1118..1489
                     /locus_tag="VFJ67_06000"
     CDS             1118..1489
                     /locus_tag="VFJ67_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289975.1"
                     /translation="MKSTRLKISVSQDGLLPQETAEIATLEFPALPKKLLKALGFERV
                     DNKTYVGVLPVFINLGEKDQEIEIELGVTLPEMRKKIIEIVKTPARLIAVAEDRKPPA
                     PAGRKKKAPVRTASRRATSKR"
     gene            1504..2979
                     /gene="guaB"
                     /locus_tag="VFJ67_06005"
     CDS             1504..2979
                     /gene="guaB"
                     /locus_tag="VFJ67_06005"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7289976.1"
                     /translation="MRVENVEECLTFDDVLLVPAYSEILPNDVDVSTRLTRNIKLNIP
                     LISAAMDTVTESSTAIAMAQEGGIGIIHRNLDIQYQAAEVKRVKKYESGMFVNPLTVR
                     PKHKVSDALEIMLKQNITGLPVTKEDDTLLGIITFRDLRFEKNLDFKVEQIMTPKKRL
                     ITVGEGTTLEEAKELLHKYKIEKLPVVDKEFRLRGLITMKDIEKIEKYPTASKDSMGR
                     LRCGAAVGVGPDREERIDALIDVGCDVIVIDTAHGHSKKVIDSIKSTKSNFPKVELIA
                     GNIGTGEGADAVIKAGADGVKVGVGPGSICTTRVIAGIGVPQVSAIQDVTRVSELHDI
                     PVIADGGIKYSGDIVKALAAGAHSVMIGNLFAGTDEAPGEVILFQGRTYKVYRGMGSI
                     EAMKKGSKDRYAQNLGDDMIDSKLVPEGIEGRIPYRGPLGGAIYQLIGGLRAGMGYTG
                     CGTIEELRSNGKFIKVTNAGLREGHVHDVIITKEAPNYRIE"
     gene            3054..>4514
                     /gene="guaA"
                     /locus_tag="VFJ67_06010"
     CDS             3054..>4514
                     /gene="guaA"
                     /locus_tag="VFJ67_06010"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7289977.1"
                     /translation="MRETILVLDFGSQYTQLIARRIRELGVYSEIKPFSTPLKDIESM
                     KPKAIILSGGPASVWDDNSPTVDRELFDLGIPVLGICYGMQLTVQLMGGIVERSKVRE
                     FGPATLEITNGFDLLSGVPDKSDIWMSHGDRVLELPPGFESIACSENSPTAATKNREM
                     RIFGTQFHPEVVHTKFGREILSNFVFKIAGCKGEWTPRSFVETAIKEIRQSVGNATVI
                     CGLSGGVDSAVAAVIIQRAIWDKLRCIFVDTGLLRMNEAEEVVESFRDMGLNLVHVDA
                     RERFLARLRGVTDPERKRKIIGEEFVNVFEEEASKIKDAKFLAQGTLYPDVIESVSVK
                     GPSATIKSHHNVGGLPEKMNLKLVEPLRELFKDEVREVGRELGVPDEILGRHPFPGPG
                     LGIRIMGEIDEERLEILRQADRIFIEEIKKGGVYDDIWQAFCVFVPVKTVGVMGDERT
                     YENVIALRAVTSQDGMTAKWARIPYDLLEKISVRIIN"
BASE COUNT         1199 a         1155 c         1351 g          809 t
ORIGIN      
        1 aagcctcagg ataaaagatg agcctgtcga ccacaccacc agattaccga agatgttcga
       61 ggacgagggc acggacgacg agctcgtaat aatgaaagat accgatattc cggaagcaga
      121 cgggttgaat tccgacctgc agcggactca gacgaatgag cgggactacg taaggcattc
      181 gactgaatct ctgggcgtgg acatgggtgt tctcgcgagc cgcccgaccc cggcggcggg
      241 gtacggcggc ggacccgggc cctcgtaccg cgaccaggtg aggatatatt cgtcgggaag
      301 ccggtcgagc ggcggttttt tcgccagggc ggcatacacg ctactgatac tgatcgtctt
      361 cgccgttatt ataggcgcct cctacatcat actctccaat acaggcgtca tcccgaggga
      421 aaccgccgat tccatcgaaa tgtatgcgag gtctcttata ccggtcagca tatctgggtc
      481 tctcgggaat gaggtagtca taactgccca cagcggtaaa tggctcgata cgaggaacgg
      541 accgatgtac gtcatatcgg ggaccgtaac aaatgaatcc ggccgccctg tcagctacat
      601 aaaaatcagg agcgaattca tatcggacgg acagaccgtt tacgataaca tggtttatgc
      661 tggtaatacg ttcacggaga atgagctcag ggtatccccg ctgagcgaca cactcctcaa
      721 gctcaaaaag aggtcgggcg atatagatta ttataatacg gaaaagcttg cgggccttga
      781 ttcgaacgtg cagccggggg agtccatacc cttttttgcg gtatatccgg cttcggggag
      841 gatgctcggc ctaaaataca acctcgatgt cgtcggttac gaatgacggg cgcaaacggc
      901 ccggcccccc ctgaaaggag cctgaagcgc ggcctgcgcg cggaatatcc ttgataaatc
      961 tttcataatc aattaaaata ttctgcttaa tagcggaata agagaattac ctttcaatct
     1021 gattcgggga taaaacagaa attcattttt ggcgtataat taagctttta tcaaatatgg
     1081 aagggatgtt tagcatcccc gcaaggtgag ggaatatatg aaatcgaccc gtcttaagat
     1141 aagtgtaagc caggacggcc tgctgcccca ggaaacggcg gagattgcga cactcgagtt
     1201 ccccgcattg cccaagaagc tgctcaaagc cctcgggttc gagagggtcg ataacaagac
     1261 ctacgtcggc gtactccccg tattcattaa cctgggcgaa aaggaccagg aaatagagat
     1321 cgagctcggc gtgacgctgc cggaaatgag aaagaagatc atagaaatcg tgaaaacccc
     1381 ggcaaggctc atagccgtcg ccgaggatag aaaacccccg gctccggctg gcaggaaaaa
     1441 gaaggccccg gtaagaacag cctcaagaag agcgacatcc aaaaggtaaa caaggaaaat
     1501 cctatgcgtg tcgaaaatgt ggaagaatgt ctaacattcg atgacgttct tttagtcccg
     1561 gcctattcgg agatactgcc gaacgacgtc gacgtatcca cacgtctcac gcgcaacatc
     1621 aagctcaaca taccgcttat cagcgccgcc atggacacgg tgaccgagtc gagcacggcg
     1681 atagcgatgg ctcaggaagg cggcatcggg ataattcaca ggaacctgga cattcagtat
     1741 caggcggcgg aagtcaaacg ggtgaaaaaa tacgagagcg ggatgttcgt gaacccactt
     1801 acggtcaggc cgaagcacaa ggttagcgac gctctcgaaa taatgctcaa acagaatatc
     1861 acgggtctcc ctgtcacgaa ggaagacgat acgctcctgg gaatcatcac tttcagggac
     1921 ctgcgttttg aaaaaaacct cgatttcaag gtcgagcaga taatgacgcc taagaagagg
     1981 ctcatcacag tcggagaggg aactactctc gaagaagcga aggagctgct ccacaaatac
     2041 aagatagaga agctcccggt ggtcgataag gaattcagac tccgcgggct cataacgatg
     2101 aaggacatag agaagattga gaagtacccc actgcgtcca aggatagcat ggggaggctc
     2161 aggtgcgggg cggccgtcgg agtgggcccc gacagggaag agaggataga cgccctcatc
     2221 gacgtcgggt gcgacgtcat agtgatagac acggcccacg gccattcgaa gaaagtgata
     2281 gattcgataa aatcgaccaa gtccaacttc cccaaggtcg agctcatagc gggcaacatc
     2341 ggcacaggcg aaggagcaga cgctgtcatt aaggcggggg cggacggtgt gaaggtcggc
     2401 gtgggacccg gctccatatg caccacgcgg gtcattgccg gtataggtgt gccgcaggtc
     2461 tcggccatac aggacgtaac cagggtctcc gagctgcacg atatacccgt aatcgccgac
     2521 ggcggaataa aatattcggg cgatatagta aaagcgcttg ctgccggcgc tcattccgta
     2581 atgataggaa accttttcgc ggggactgac gaggccccgg gcgaggtcat actgttccag
     2641 ggcaggacgt acaaggtgta caggggaatg gggtcgatcg aggccatgaa gaaaggcagt
     2701 aaggacaggt acgcccagaa cctcggggac gacatgatcg attcgaagct cgtgcccgaa
     2761 ggcatagagg gaaggatacc gtacagaggg ccgctcggcg gcgcgatcta ccagctcata
     2821 ggcggactca gggcgggtat gggctacacg ggatgcggca ccatagagga gctcaggagc
     2881 aacgggaagt ttataaaagt cacgaacgcg ggactgagag agggccatgt ccacgacgta
     2941 atcatcacga aagaggcccc gaattacagg atcgaataat ccgcacggtc cgcagccagt
     3001 tttaatcatc tgctccacca acaccggaaa cggccgggca tttcaacagc gacatgcgcg
     3061 agaccatact agtactagat ttcggctccc agtatacgca gctcatcgca cgcaggataa
     3121 gggagctcgg cgtctactcc gaaataaaac cattcagcac cccgcttaaa gacatagaga
     3181 gcatgaaacc gaaggcgata atcctctcgg gcggccccgc gagcgtctgg gacgacaact
     3241 ccccgaccgt cgacagggag ctgttcgatc tggggatacc ggtcctcggg atatgctacg
     3301 gcatgcagct cacggtgcag ctcatgggcg gcatagtcga gcgctcgaag gtgcgggagt
     3361 tcgggcccgc gacgctcgag ataacgaacg gattcgacct cctgagcggc gtcccggaca
     3421 agagcgacat atggatgtcc cacggcgacc gcgtgctgga gctgccccca ggtttcgaat
     3481 ccatagcctg cagcgagaat tccccgaccg ccgcaacgaa aaaccgggag atgaggatct
     3541 tcgggacaca gttccatccg gaggtcgtgc atacgaaatt cggcagggag atactatcga
     3601 acttcgtctt caagatcgcc ggatgcaagg gcgagtggac gcccaggtcg ttcgtcgaaa
     3661 ccgctataaa ggagataagg cagagcgtcg ggaacgcgac agtcatatgc gggctctcgg
     3721 gcggcgtgga ctccgccgtg gccgccgtca taatacagcg cgcgatatgg gacaagctcc
     3781 gctgcatatt cgtcgacacc gggctcctca ggatgaacga agcggaggag gtagtggaat
     3841 cgttcaggga tatggggctc aacctcgtgc acgtggacgc aagagagagg ttcctcgcga
     3901 ggctccgggg cgtcacggac cctgaaagga agaggaagat cataggcgag gagttcgtta
     3961 acgtgttcga ggaggaggcg tcgaaaataa aggacgcaaa attcctggcg caggggacgc
     4021 tctacccgga cgtcattgag agcgttagcg taaagggccc ttcggcgact atcaaatcac
     4081 accacaacgt aggcgggctg cctgaaaaga tgaacctcaa gctcgtcgag ccgctgagag
     4141 agctcttcaa ggacgaagtg agggaagtgg gccgcgagct cggagtgccg gacgagatac
     4201 ttggccgcca tccgttcccc ggcccggggc tcggcatcag gatcatgggc gagatagacg
     4261 aagagaggct cgagatactg agacaggccg acaggatctt catcgaggag ataaagaaag
     4321 gcggcgttta cgatgatatc tggcaggcgt tttgcgtgtt cgtgccggtc aagaccgtag
     4381 gcgtgatggg ggacgagagg acttacgaga acgtcatagc gctccgggcg gtcacgagcc
     4441 aggacggcat gacggccaaa tgggcgcgca tcccttacga ccttctcgag aaaatctcgg
     4501 tccgcatcat caac
//
LOCUS       DASSJH010000280         4899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_825012, whole genome shotgun sequence.
ACCESSION   DASSJH010000280 DASSJH010000000
VERSION     DASSJH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825012"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFJ67_06015"
     CDS             <1..322
                     /locus_tag="VFJ67_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HET7289978.1"
                     /translation="VPSGIRTNLRLLQVGDIISLALCHGWESMEIPDVPVDYEGGSAK
                     LFLKSGDGFNFTLSPYPFSETLLDLSVEGLRLERKSYSDDSDLRSALDNAPRVTLDFT
                     IGKG"
     gene            332..1639
                     /locus_tag="VFJ67_06020"
     CDS             332..1639
                     /locus_tag="VFJ67_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET7289979.1"
                     /translation="MQERVFNAFDEKDRPSAELIEDCVHCGFCLSACPTYRETGNELD
                     SPRGRIYLMKTASEGKIPMSGSLVKHLDLCLGCLACEPACPSGVQYGRLIEAGRSQIE
                     RRYERPLPERIQRSFIFSLFPYPGRLKLVLPFFYLYQKLGIKGLIDSTGILKSVSPGL
                     ARMEEMLPEVSSPLVPRALPEVVPARGKKRFRVALLTGCVQSVFFSKTNEATVRVLAE
                     NGCDVIIPRNQGCCGALSVHSGRLHEGRDFAKHLIRSFDALDVDAIIVNSAGCGSTMK
                     EYGDLLEHDPAFAERAHKISEKVRDVMEFLGDIGLAGELKALDMKITYQDACHLGHAQ
                     RIKDQPRSVLGQIPGVELTELTESELCCGSAGIYNMVEPGMSQRLLERKIKHIRETGA
                     DCLVAGNPGCLLQIGRGLKQYGLEIKTAHPVELLDRSYRGAAE"
     gene            complement(1651..2148)
                     /locus_tag="VFJ67_06025"
     CDS             complement(1651..2148)
                     /locus_tag="VFJ67_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289980.1"
                     /translation="MRPSAAAAVVLTIFLSVLMAGCRAGNENAEFPENCLQTGFEYKD
                     GSVILNTDRKPSQGLYLLNNKSDSDYWITHPVKDPGASAGWTSDINPGNWSAFTVNIP
                     GFELACMKTGQSSMDTLSCEKVLRVCRIQDPVFKPDMNGNYWVSEDKPQGAVLDEIKG
                     RGIGF"
     gene            2291..2860
                     /locus_tag="VFJ67_06030"
     CDS             2291..2860
                     /locus_tag="VFJ67_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289981.1"
                     /translation="MRRFSLLLVAFLIFTFYSCDSVEDFFRGPVDPLIDEQTIESQDF
                     NLTTPNYGIQVAIDSGVSAGTEHVLDLLDNRAEEFLQCQFGDSQIGFENFPLDGTVVP
                     PLSELRVYVVSNRFECEAEDRSVCGGIYFFDIDAIAISEGGFEGCGEFSVWKHELAHR
                     YGLEADHSNQSDFNSCSKPQTCDPGNIFD"
     gene            complement(2876..3451)
                     /locus_tag="VFJ67_06035"
     CDS             complement(2876..3451)
                     /locus_tag="VFJ67_06035"
                     /inference="COORDINATES: protein motif:HMM:NF018964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HET7289982.1"
                     /translation="MKGVILFLSLFYLTFYIPMSLTFYAPWWMKLNCNWHNRCAIIGY
                     DNAYTGIEELAAFFRHQGGLDHFYTAKEKTHLREVRGMFDKMLIIGVAALVLLALTYD
                     RKRIGRFAIINAAIILSLLLVLPFFTTFWRDVFHPLLFKNNLWINNRYDLSFYIMPRQ
                     FFKYTVALLVVLSVSLNVIIRLLFRKSAKNA"
     gene            complement(3500..4708)
                     /gene="nhaA"
                     /locus_tag="VFJ67_06040"
     CDS             complement(3500..4708)
                     /gene="nhaA"
                     /locus_tag="VFJ67_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET7289983.1"
                     /translation="MAVIPSRAVNAIREFLNLESAGGIILVGAAVLALILNNSPLSGL
                     YDLLLQTPVEMRVGALHLAKPLLLWINDGLMAVFFLLIGLEIKREVMDGQLSSLSQIA
                     LPGIAAVGGMAVPALIYVFFNFNYPGALHGWAIPAATDIAFALGVMTLLGSRVPLSLK
                     LFLLTLAIIDDLGAIVIIAVFYTGKLSLLSLALAGAAAAALVVMNVVGVKRVAAYVIV
                     GVVLWICVLKSGVHATLAGVALAFAIPLRTKEDEPCPLRDLEHTLHPWVAFGIMPVFA
                     FANAGVSLEGMSLFTLFEPVPVGVALGLFLGKQFGVFGFAKLCIKLGLARMPDGASWL
                     SLYGTSVLCGIGFTMSLFISTLAFDPGASDEGISARLGILAGSFVSAVCGYFILKYSL
                     ARQKTAANEQ"
BASE COUNT         1105 a         1350 c         1393 g         1051 t
ORIGIN      
        1 cgttccctcc ggaatccgga cgaacctcag gctcctacag gtgggggata tcatatcgct
       61 cgccctctgc cacggctggg aatctatgga gataccggac gttcccgtcg attacgaggg
      121 cggctcggcg aagctcttcc ttaaatccgg ggacggcttc aactttactc tatcacccta
      181 tccgttttcg gagacattgc tcgatctgag cgtcgagggg ctcaggctcg aaaggaaaag
      241 ttattcggac gacagcgatc tgagaagcgc gctcgataac gctccccgcg tgacccttga
      301 tttcacaatc ggaaaaggct aaacctttta tatgcaggaa agggtgttca acgctttcga
      361 cgagaaagac aggccgagcg ccgagctcat agaggactgc gtccactgcg gcttctgcct
      421 gtccgcatgc cctacttacc gggagaccgg gaacgagctc gactccccga ggggcaggat
      481 ttatctcatg aagacggctt ccgagggcaa gattccgatg tcgggctctc tcgtgaagca
      541 cctcgacctc tgcctcggat gtctcgcctg tgagcccgcc tgtccatcgg gcgtgcagta
      601 cgggaggctc atcgaggcgg gcaggtccca gatcgaaagg cggtacgaga ggccgctgcc
      661 cgaaaggata cagaggtctt tcatcttctc tctcttccct tatcccggca ggctcaagct
      721 ggtcctgccg ttcttctatc tttatcagaa gctcgggata aagggcctga tcgattcgac
      781 agggatactg aagtcggtct ctcccggcct tgcgcgtatg gaggagatgc tccccgaggt
      841 gagctcccct ctcgtgccgc gcgcgcttcc ggaagtcgtg cccgcacggg gaaagaagcg
      901 cttccgtgtg gcgctcctta ccggatgcgt gcagagcgtt ttcttctcaa agactaacga
      961 agccacggtc agggtcctgg ccgagaacgg gtgcgatgtg atcattccgc gtaatcaggg
     1021 atgctgcggc gcgctttcgg tccattcggg caggctccac gaagggaggg acttcgcgaa
     1081 acatcttatc cggagcttcg atgccctgga cgtagacgcc ataatagtaa actccgcggg
     1141 ctgcggctcg acgatgaaag aatacgggga tctgctggag cacgaccctg cgttcgcgga
     1201 gcgcgcacat aaaatcagtg aaaaggtgag ggacgtcatg gagttcctgg gggacatcgg
     1261 tcttgcgggc gagctgaaag cgctcgatat gaagatcacg tatcaggacg cctgtcatct
     1321 gggtcacgcg cagaggataa aagaccagcc gcggagcgtg ctcggccaga tacccggggt
     1381 cgagctaacg gagcttaccg agtccgagct ctgctgcggg agcgccggca tatataacat
     1441 ggtcgagccc ggcatgtcgc aaaggcttct cgagcgcaag atcaagcata ttagagagac
     1501 aggggcggat tgcctcgtgg ccgggaaccc cggatgcctc cttcagatcg gaaggggctt
     1561 aaagcagtac ggtctcgaaa tcaaaacggc ccacccggtc gagctgctag accggtccta
     1621 caggggagcc gcggaataac ggctcttcgg tcagaagccg atcccacgtc cctttatctc
     1681 gtccagcacc gctccctggg gcttgtcttc agagacccag taattcccgt tcatgtccgg
     1741 cttgaatacg gggtcctgaa tcctgcagac acgcagcacc ttttcgcatg agagcgtatc
     1801 catcgagctc tgtcccgtct tcatacaggc gagctcgaac ccggggatgt tcaccgtgaa
     1861 cgccgaccag ttgccggggt tgatgtcgga cgtccacccg gcgctcgcgc ccgggtcctt
     1921 cacaggatgc gtgatccagt agtcgctgtc cgatttgttg ttaaggagat agaggccttg
     1981 ggacggcttc cggtccgtgt tgaggataac gctgccgtct ttatactcga accccgtttg
     2041 caagcaattc tcggggaatt cagcgttttc attgccagcc ctgcaacccg ccattaaaac
     2101 cgataagaag atcgtgagca cgactgccgc cgctgcgctc ggtctcattt tttatctccc
     2161 gtctttaatt tatcctttaa gtttactcta aagtttgtga tttttagatg aatttattcg
     2221 atagcatacg gaaaatctgt cggttatcat atttgtaatc tttatcaacc tgaattaaag
     2281 gatgggtctt atgagacgat tctccctttt gctcgttgca ttcctcatat ttacattcta
     2341 ctcctgcgat agcgtggagg actttttccg cggcccggtc gatcctctca tcgacgagca
     2401 aacgatcgaa tcccaggatt tcaaccttac gacgcccaat tacggcatac aggtggcgat
     2461 agattccgga gtgagcgccg gcactgagca cgtgctcgac ctgctcgata acagggccga
     2521 ggaattcctg caatgccagt tcggcgattc tcagatcggg ttcgagaatt tcccgctcga
     2581 cggcaccgtc gtgccgcctc tgtcggagct tagggtgtac gtcgtgtcca accgtttcga
     2641 atgcgaggct gaggaccgca gcgtgtgcgg cggtatctac ttcttcgata tagacgcgat
     2701 agctatttcc gaagggggat tcgaggggtg cggggagttc tccgtctgga agcacgagct
     2761 cgcacacagg tacggcctcg aggccgacca cagcaaccag tcggatttca actcatgctc
     2821 aaagccccag acgtgcgacc ccggcaacat attcgattga tcaggcgggc ggggatcagg
     2881 cattcttcgc gcttttacgg aataacagcc ggataataac gttcagcgaa acggagagca
     2941 cgaccaggag cgctaccgtg tatttgaaaa actgcctcgg cattatgtaa aaggaaaggt
     3001 cgtacctgtt gttgatccac aggttgttct tgaaaaggag agggtggaac acgtccctcc
     3061 agaaggtcgt gaagaaaggg agcacaagca gaagcgagag tataatcgct gcattgatta
     3121 tagcgaagcg cccgatcctc ttcctgtcgt aagtgagcgc gagaagtacg agcgcggcga
     3181 cgccaattat gagcatctta tcgaacatcc cccgcacttc ccttaaatga gttttctcct
     3241 tcgcggtgta gaaatggtcg agtcccccct ggtgtctgaa aaacgcggcg agctcttcaa
     3301 ttcccgtgta agcgttatcg taacctatta ttgcgcagcg gttatgccaa ttgcaattca
     3361 gcttcatcca ccagggcgcg taaaaggtca gagacatagg tatgtagaac gtcaggtaaa
     3421 agagggaaag aaaaagaatt acacctttca aacggttcag catggcgatg cgaaaaagac
     3481 gattttctcc cgactcgttt tattgttcat tcgctgcggt cttttgcctg gcgagagaat
     3541 atttgagaat gaaatatccg cagaccgccg agacgaacga tcccgcgaga atgccgagcc
     3601 ttgccgatat cccctcgtcc gacgcgccag ggtcgaaggc aagcgtgctt atgaacaagc
     3661 tcatcgtgaa cccgatcccg cacagcacgg acgttccgta tagtgagagc caggacgccc
     3721 cgtccggcat cctagcgagg ccgagcttta tgcagagctt cgcgaatccg aacactccga
     3781 actgttttcc gaggaagagc ccgagcgcga cgcccaccgg aacgggctcg aacagggtga
     3841 agaggctcat cccttcgagc gacactccgg cgttggcgaa tgcgaatacg ggcattatgc
     3901 cgaaagccac ccagggatgg agggtgtgct cgagatcgcg cagcgggcat ggctcgtcct
     3961 ccttcgtcct gagcggtatg gcgaacgcga gcgctacacc ggcgagagtc gcatgcacgc
     4021 ccgatttaag gacgcatatc cagagcacta cgccgactat cacgtaagcg gctactctct
     4081 tcacgcccac tacgttcatc acaacgagcg ccgccgcagc cgcccccgca agcgccagag
     4141 acaggagcga aagcttcccc gtatagaaga cggctattat cactatcgcg ccgaggtcgt
     4201 ctatgattgc gagcgtgagc aggaagagct ttaacgatag agggaccctc gagcccagaa
     4261 gcgtcatgac gccgagcgcg aaagcgatat cggtcgcggc gggtatggcc cagccgtgaa
     4321 gcgcccccgg gtaattgaaa ttgaagaata cgtatatcag cgccggcact gccatgcccc
     4381 cgacggcggc gatccctggg agggcgattt gcgagaggct cgacagctgg ccgtccatca
     4441 cctcgcgttt tatctcgagg ccgatgagca ggaagaatac ggccatcagg ccgtcgttta
     4501 tccagaggag gagcggcttc gccagatgaa gggctcctac cctcatctcc acgggcgttt
     4561 gcagaagcag gtcatagaga ccggacagtg gggaattgtt cagtatgagc gcgagcaccg
     4621 cggctcccac gaggatgatg ccgcctgccg attcgagatt cagaaactca cgtatggcgt
     4681 ttactgcccg cgaagggatg actgccatta tggctcggtc cttacctcag tgtctagatt
     4741 atacgtaagg gggcctttaa atacaaagca gcgtgacctt tgatcgcgtt aacggtgagc
     4801 tttgtagaaa aaccgtcgat gtgttaatat cctgaaactc gaaaatatca ggtgtatcag
     4861 gcggagtctc tattggaaat aggctacttt accatgccg
//
LOCUS       DASSJH010000281         4194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1037373, whole genome shotgun sequence.
ACCESSION   DASSJH010000281 DASSJH010000000
VERSION     DASSJH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037373"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..2274
                     /locus_tag="VFJ67_06045"
     CDS             214..2274
                     /locus_tag="VFJ67_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7289984.1"
                     /translation="MTEAEEPNKGRKTRDLSIIRWLYSFTGPYRTLMAVALVLMILTA
                     LMELAVPYITKIAVDKYIYPSWGIAHFKNNAKDREFERSVRQKYPSLVIPIGEDSYLI
                     DMSGLDNADRNDLEKLGTVPEERYLVIDPSRLDKEKLARADEIIGKNSGLFMETGGVR
                     YISYSSLKKLGKDDIRALRSEDIKEVINLSAVVFACLTGIFILSSIFTYLLNYSGHRI
                     MHRMRTDVFSHILSLPQPFFDKNPVGRLTTRVTSDINAINEMYTSVAVQFIKDVLVVI
                     GVFVIMFRLNMKLTLFVAAFTVVMGLFAGLFRMKLKTVYRDVRRSIAKLNAFVQESVR
                     GIVLIKLYNREMENFEKFKETNRENFKANMDQLWVYATFRPFIEFMSVFTVAFILWYG
                     GIGVLRLELTLGALLAYLYYVRMLFRPIMELAERYNIFQSATAASENLFDLAGVQPER
                     REGITETSVRGKLEFRNVWFSYNDKDWVLRDVSFTIEPGETVALVGLTGSGKTTIVNL
                     ILKFYDAQRGQILFDDRDIRGLSPEFLRGNISAVFQDLFLFGKNINDTESEEFSKVVN
                     MDRLKANGNTFSAGEKQLVSLGQAFSKNSKFLILDEATSNIDASIERNIQKALRNEAR
                     DQTTLIIAHRLSNVRHADRIMVIHKGEISETGTHSGLLEKKGIYYNLYRLQNEIHSSS
                     HLRD"
     gene            2302..3501
                     /locus_tag="VFJ67_06050"
     CDS             2302..3501
                     /locus_tag="VFJ67_06050"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HET7289985.1"
                     /translation="MEFATLEKQMDETRAYLKRRESFDESLLGAVVSPYRISPLGAHI
                     DHQGGPVLGMTIDARSVLVFVPNDERRVRLYSMNYPGVTEFDIDNIKTPGRDDWGRYA
                     MGAAKVLDEYRGIARGFTGALSGALPSAGLGSSASSGLAYLKTLALLNGHELGPEEYA
                     ELDRRIENDYLKLSNGILDQSSIVYGKRESLLYIDTVTGHTASYPRPKNWDDFQILIV
                     YSGIPRDLTSSGFNTRVEECRKAAGLLGIMGGLRSAKALSYIPERVFREKSPKLPEDL
                     RKRAEHFFSESERVTMGIAAWNRGDMKLFGQLMNESCRSSFVNYEVGSPELIALQEII
                     STTEGVYGSRLNGGGYGGSVTAFVKRDFSEDGASVILEKYRNKYPGPGADADAYFARS
                     DDGLRLL"
     gene            3498..>4194
                     /locus_tag="VFJ67_06055"
     CDS             3498..>4194
                     /locus_tag="VFJ67_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7289986.1"
                     /translation="MNAILLCAGFATRMTPLTKDFPKALLPVGGKPVIDHLIDQFLDF
                     RGLDSVYVVTNDRFFKPFLEWGEKSSRTITGRGISLHILNDGAGRPESRLGAAGDLGF
                     VVKHMIPGGDTVVAAGDNIFRFPLGPCWEKFTTGNKSFVLALPTDDALKLKRTGVLEL
                     DEKGRVLAFHEKPETPPSNLACPAIYFLKPGSLRLIDEYLSSPGARDEIGYFISYIAG
                     HGDIYALRTGGEAI"
BASE COUNT         1093 a         1102 c         1166 g          833 t
ORIGIN      
        1 gattgcgtaa aattccgccg caaaacgccg gaatcgattc tcagtccttt tgattttttg
       61 tgacgggcaa atagaattat actacttaag acaagtcttt tacaaagggg aagaaggcgg
      121 gccgctttaa taatccggac tcactaaaca gcaggttata ctatgccttg atcaggcgta
      181 tataaacacg accgcggagc agcaactttg gcagtgaccg aagcagagga accgaataaa
      241 ggcaggaaga cacgcgacct cagcataatt agatggctct acagctttac agggccttac
      301 aggaccctga tggcagtcgc tctcgtcctt atgatcctga ccgccctcat ggagctcgcg
      361 gtaccgtata taaccaagat cgccgtggac aaatatatat atccgtcctg ggggatcgcc
      421 catttcaaga acaacgcgaa ggacagggag ttcgaaaggt ccgtcaggca aaagtatccc
      481 tccctcgtta tacccatagg ggaggattca tacctcatcg atatgtcggg gctcgataac
      541 gcggacagga acgacctcga aaagctggga accgttccgg aagagagata cctcgtcata
      601 gatccgtcgc gcctcgataa agaaaagctc gcccgcgctg acgaaataat cggaaagaac
      661 agcgggctct tcatggaaac gggcggcgtc cgctacataa gctactcatc gctcaagaag
      721 ctcggaaagg acgacatacg cgctctcagg agtgaagaca taaaggaagt tatcaatcta
      781 tcggctgtcg tgttcgcgtg cctgaccggt atcttcattc tgagctccat cttcacctac
      841 ctgctcaact attcgggaca caggataatg cacaggatga ggacggacgt gttctctcac
      901 atactcagcc tccctcagcc gttcttcgac aaaaaccccg tagggaggct gacgacgagg
      961 gtgacaagcg acatcaatgc gatcaacgaa atgtacacat cggtcgccgt gcaattcatt
     1021 aaagacgtgc tcgtggtcat aggcgttttt gtgatcatgt tccggttgaa catgaagctc
     1081 accctcttcg tcgcggcttt tacagtggtt atggggctat tcgccgggct cttcagaatg
     1141 aagctcaaga ccgtatacag ggacgtaagg agatccattg cgaaattgaa cgcgttcgtt
     1201 caggaaagcg tgagggggat cgtgctcata aagctctata acagagagat ggagaacttc
     1261 gagaagttca aggagaccaa cagagagaat ttcaaggcca acatggacca gctctgggtc
     1321 tatgcgacgt tcaggccctt tatagaattc atgagcgtat tcactgtcgc gttcatactc
     1381 tggtacggcg gtatcggcgt gctcaggctc gagctgaccc tgggcgctct gctagcctac
     1441 ctttactacg tgaggatgct attccgtcct atcatggagc tcgccgaaag atacaacata
     1501 ttccagtccg cgaccgccgc gagcgaaaac ctcttcgacc tcgcgggcgt gcagcccgaa
     1561 cggagagaag gcataacgga gacgagcgta agggggaagc tcgaattcag gaacgtatgg
     1621 ttctcgtaca atgacaaaga ctgggtcctc agggacgtat cgttcacgat agagccggga
     1681 gagaccgtgg cgctggtcgg actcacgggg tcgggcaaga cgacaatagt caacctgata
     1741 ctcaaattct atgacgcaca gaggggacag atactattcg acgacaggga catccgcggg
     1801 ctcagcccgg aattccttag agggaatatc tccgccgtat tccaggacct ctttctattc
     1861 gggaagaata taaacgatac ggaaagcgaa gaattctcga aggtggttaa catggatcgg
     1921 ttaaaggcga acgggaatac gttctcggca ggggagaaac agctggtctc gctcggacag
     1981 gcgttttcta aaaactcgaa gttcctgata ctcgacgagg cgacttcgaa tatcgacgcg
     2041 agtatagaaa ggaacattca gaaggcgctc agaaacgagg cgcgcgatca gacgactctc
     2101 ataatcgcac acaggctctc taacgtgagg cacgccgaca ggataatggt catacataag
     2161 ggtgagatat cggaaaccgg cactcattcg ggcctcctcg aaaagaaggg gatatattat
     2221 aacctctacc ggctccagaa cgagatacac agttcttcgc atctcaggga ttgaccggct
     2281 ttcaggagac ggacacacac aatggaattc gccacactcg aaaagcaaat ggatgagaca
     2341 cgagcgtatt taaagagaag agaaagcttc gacgaatccc ttctcggggc agtggtctcc
     2401 ccctaccgca tatcccctct gggcgcccac atagaccacc agggcggccc ggtgctcggc
     2461 atgactatag acgcccggtc ggtcctcgtg ttcgttccga acgacgagag aagagtcagg
     2521 ctatacagta tgaactaccc cggggtaacg gaattcgaca tcgacaacat caagactccc
     2581 ggcagggacg actggggaag atacgcgatg ggggcggcca aagtgctcga cgagtaccgc
     2641 gggatcgcga gaggattcac cggcgcactc tcgggcgcgc tcccctcggc cggcctcggc
     2701 tcctcagcct catccggcct cgcatacctt aagacgctcg ccttacttaa cggccacgag
     2761 ctcggcccgg aggaatacgc cgagctcgac agaaggatcg agaacgatta tctgaagctc
     2821 tcaaacggca tcctcgacca atcctcgatc gtttacggta aaagagagag cctcttgtac
     2881 atagatacgg ttaccggaca cacggcgtca tatccgaggc ccaagaactg ggacgacttt
     2941 caaatactga tcgtctattc cggcataccg cgcgatctca cttcatcggg attcaacacg
     3001 cgcgtcgagg aatgcagaaa ggcggcgggg ctcctcggca taatgggagg gctcagatcg
     3061 gcgaaggccc tgtcgtacat tcccgagaga gtgttccggg aaaaatcgcc caagctgccc
     3121 gaggatctga ggaagagggc cgagcacttc ttctcggaat cggaacgggt gacgatgggt
     3181 atcgccgcat ggaacagggg cgacatgaaa ttattcggac agctcatgaa cgaatcatgc
     3241 cgaagctctt tcgtcaatta cgaagtaggc agccccgagc tcatcgcgct ccaggagatc
     3301 attagcacga ccgagggcgt ttacgggagc aggcttaacg ggggaggata cggcgggtcg
     3361 gttaccgcat tcgtgaagag agacttctcc gaggatgggg cttccgtgat actggagaaa
     3421 tatagaaaca aatatcccgg gcctggcgcg gatgcagacg catatttcgc gagatcggac
     3481 gacgggttga ggctcttatg aacgctatcc tcctttgcgc gggattcgcg acgaggatga
     3541 caccgcttac gaaagatttc ccaaaggctc tcctcccggt cggcggaaag ccggttatag
     3601 accaccttat agatcagttc ctcgatttca gaggactcga ttccgtatac gtagttacga
     3661 acgaccggtt cttcaagccg tttctcgaat ggggcgaaaa gagcagcagg acgataaccg
     3721 ggcgcgggat aagcctccat attctcaacg acggagccgg ccggccggag agcaggctgg
     3781 gggcggcggg cgacctggga ttcgtggtga aacacatgat accaggcggg gacacagtcg
     3841 tcgcggccgg ggacaatata ttccgcttcc cgctcggacc ctgctgggaa aaattcacta
     3901 caggcaacaa gagctttgta ctcgcgctcc cgaccgatga cgccctaaaa ctgaaaagaa
     3961 ccggggtgct tgaactggat gaaaagggac gggtcctcgc gttccatgag aagcccgaga
     4021 ccccgccctc gaacctcgcc tgcccggcta tctacttcct caagcccgga tcgctccgcc
     4081 tgatcgacga atacctgtcg tcaccgggag cgcgggacga aatcggatat ttcatttcct
     4141 acattgccgg gcatggtgat atatatgcgc tcagaaccgg gggagaagcg atag
//
LOCUS       DASSJH010000282         6850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1366864, whole genome shotgun sequence.
ACCESSION   DASSJH010000282 DASSJH010000000
VERSION     DASSJH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366864"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFJ67_06060"
     CDS             complement(<1..731)
                     /locus_tag="VFJ67_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289987.1"
                     /translation="MTERLLSKSGGNGTTVQETVFSIAAFILLLFCIGNLLLGISRVN
                     KPYPGFFFYKNLVITDISPKAVNGETLKRFKDEVRKVNGITVKTPDDVFGIINKYPVG
                     TDIQYTIERNNTTFDVVIPIEKLTPKEMILTFGIIYMIGIVFLIIGVMVLQMKPYLRA
                     SKAFFLFCASICIWFVGSFDAQAVYLFNQLTFFAWIFSPLFGIYLMFIFPSDTKLKGI
                     THNLASILFLFISAMLFVLQFIFFY"
     gene            complement(736..3495)
                     /locus_tag="VFJ67_06065"
     CDS             complement(736..3495)
                     /locus_tag="VFJ67_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7289988.1"
                     /translation="MGIVIRESGDRRKHERIPVNFQVYSKNSGNMIGLAKDLSSKGLF
                     IQTEDDYEPGTKLLLECNLSNSHPVKAYCEVKRHETNGTGEHGIGVEFINIYDSDRAK
                     LESYIEKSKHALNSDDYYLSDFADIPDEDLFKKTEVFWQYKLDMESKGYIRYRRPLAS
                     PSAHRVIVDDDFTGKKKEMIMMGSNNYLGLTAHPTVMKIAKEKIDQYGAGAGSVPLLA
                     GTFDIHRELEVKLAELKGAEDAIIFPSGYVTNLGCIQALVKNEDLAVIDRLAHASIID
                     GCMLSSGSFRTFKHSDVRSLESVLKRNTDNFKGKIVIVDGVYSMDGDIAPLRQITETA
                     HRYGAKVMVDEAHATGVIGDRGKGTPSHFKMRPGEVDIVMGTLSKSLGGIGGFIASTK
                     EVVNYLRYYTRSFFFSTNFPPSVAASVLAGIEVMQSDKSLHENLWRNVKYMKENLKSL
                     GFQTGQTESAIIPVLIGDELTQKKMSRRFHEEGIYVNAIPHPAVPKGQERFRFSVMAT
                     HTREDLDRSLEVTEKLGREFGIIARPFAPGAPEPERYSVREMTSKEDIEKSVRLSWKV
                     YKDYPAWVPYFLIKDQVKLISNDYFYFRKVYGKRFVAEENGEIVATVSAFIDNYYNRY
                     HDTSAGFLGFFEALPDKEEAVGLLMRKAEEFLTGEGCREFLGPVNGVFGLFGGGLLSS
                     NYGKIPSFLQVYTQPYYHKYFTNSGLMPVKKLLHYTIDLKSPDNVKAIMKYSRESLPP
                     DVKIRQINKSDWENEVRNVVRIFNNSLAQLWGNVPFDTDEFLEFAEEFKSLISPEYWL
                     IAESGGKAVGFIGGFPQYASIFRGLDGELKPHKLLTVPLKMRAIKEGVLMIMGLTDEY
                     KGKRIGTVLLSRVCDAMIGNGYEKVAGTWVLEDNLGSRRIVENLGGKVDLHWEMYSKI
                     PALNE"
     regulatory      complement(3610..3701)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(4046..5116)
                     /gene="aroC"
                     /locus_tag="VFJ67_06070"
     CDS             complement(4046..5116)
                     /gene="aroC"
                     /locus_tag="VFJ67_06070"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET7289989.1"
                     /translation="MPGNSFGQLFRITTWGESHGEALGVVVDGCPAGLEISKEDIQSE
                     LDRRRPGQSRITTQRKESDSVEILSGVFEGKTLGTPISLMVRNEDVISKSYEDIKDTY
                     RPGHADYTYDAKYGIRDYRGGGRSSARETVGRVAAGAIAKKILSLSGIRTYGFVRQVG
                     RIVARKVDPDEIERNIVRCPDAGKAAEMIELIEKVRKEGDSIGGTVEVVSTGLPPGLG
                     SPVFNKLDADLAAALMSLGGIRGFEVGMGFEASKRKGSQVNDVMYKDRSGKLRFKTNN
                     AGGLLGGITNGEDLVVRIAIKPTSSISKVQHTVDKYGNPVELKVKGRHDPCLCPRAVP
                     IAEAMVNLVLADHLLMSRLAKI"
     gene            complement(5172..6155)
                     /locus_tag="VFJ67_06075"
     CDS             complement(5172..6155)
                     /locus_tag="VFJ67_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7289990.1"
                     /translation="MATLVTGATGFLGSHIARKLAERGDEVKLLVRKTSKTINIDDIK
                     AERVYGDVTDIESVRKALEGCDTLYHTAGIVSFKQADYKKMEEVNVGGTNNVFTAAME
                     AGVKKAVYTSSVAAIGLKPGMEPADEETPFDPSGTDIQYIKSKYYAEQEALKFFGKGL
                     PLVIVNPSIVIGAGDVYVSTSGIILWYCKRRLPGYTDGGINLVDAEDVAAGHLLAAEK
                     GKLGERYILSNRNITIKELFEVLEKVTGIPRPKMKIPYPLAYLGAFIAERIFGISAPN
                     YVAMDIDSIKGSKHYWYFDNSKAVRELGFSPRPLEETIKSTVNWFRENGYL"
     gene            6353..>6850
                     /locus_tag="VFJ67_06080"
     CDS             6353..>6850
                     /locus_tag="VFJ67_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289991.1"
                     /translation="MDLEELLRDREYDEGEKRLERALVIAEREWEKVRLSFGECGDIG
                     GFDKKDFMIGIIKEDVIIRQPLVSPTKSVSVYAPTFYPMYFINNLLAMEEKFEDRGYS
                     TSEALYAYIELATRASEKLGLEGTFAMAFGAGYGSVRTGWVAEKGFPVEREIFFNMFF
                     ESKKKD"
BASE COUNT         1426 a         1892 c         1714 g         1818 t
ORIGIN      
        1 ctgtagaaaa atatgaactg gaggacgaag agcatggccg atataaacaa aaacaatatc
       61 gatgccagat tgtgtgttat tcccttcagc ttcgtatcgg acgggaaaat gaacatcagg
      121 tatattccaa aaagcgggct gaatatccat gcaaagaacg taagctgatt gaacagataa
      181 accgcctgcg catcgaacga gcccacgaac catatacata ttgaagcgca gaacaggaaa
      241 aacgccttgc tcgcccggag atagggcttc atttgaagaa ccatcacccc gataatgaga
      301 aacacgatgc ctatcatgta tatgattccg aacgtcagaa tcatctcttt cggcgttaat
      361 ttctctatgg gaatgacgac atcgaacgtg gtgttgtttc tctcgatcgt atactgaata
      421 tcggtcccca ccgggtattt gttgattatt ccgaagacgt catccggcgt tttgactgtt
      481 attccgttga ctttcctgac ctcgtcctta aacctcttca gcgtctcgcc gttgaccgct
      541 ttaggtgata tgtcggtgat tacgaggttc ttatagaaga agaatcctgg gtagggtttg
      601 ttcaccctgc tgatacccag cagcagattc ccgatgcaga aaaggagcag aatgaacgca
      661 gcgatgctga ataccgtttc ctgaacggtc gttccattcc ctcccgactt ggacagcagt
      721 ctttctgtca taagactatt cgtttaatgc aggtattttc gaatacattt cccagtggag
      781 gtccaccttc ccgccgaggt tctccacgat ccgccgcgag cccaggttat cctccaggac
      841 ccatgtgcct gccacctttt catacccgtt tccgatcata gcgtcgcaga ccctgctgag
      901 taggaccgtt ccgatcctct tccccttgta ttcgtccgtg aggcccatga tcatcagcac
      961 gccttccttt attgctctca tcttgagcgg cacagtcagc agcttgtggg gcttgagctc
     1021 tccgtcgagg cccctgaata tagaggcgta ctgagggaac ccgcctatga agcctaccgc
     1081 cttgcctccc gactcggcga tgagccagta ttcgggggat atcaggctct tgaactcctc
     1141 cgcgaactcg aggaactcgt ccgtgtcgaa ggggacgttg ccccagagct gggcgagtga
     1201 attattgaat atcctgacta cgttcctgac ctcgttctcc cagtcggatt tgttgatctg
     1261 tcttatcttc acatccggcg gcagggattc ccgcgagtat ttcatgatcg ctttaacgtt
     1321 gtccggggat ttcaggtcta tcgtgtagtg gagcagtttt ttgacgggca tgagccctga
     1381 gttcgtaaag tacttgtggt agtagggctg ggtatatacc tggaggaagg acggtatctt
     1441 cccgtagttg ctcgagagca gcccgcctcc gaagaggccg aatacgccgt taacgggccc
     1501 gagaaattcc ctgcagcctt cacctgttag gaattcctcc gctttcctca ttagcagtcc
     1561 gaccgcttct tccttgtcgg ggagagcctc gaagaacccg aggaatcccg cgctcgtatc
     1621 gtgataacgg ttatagtagt tgtctatgaa cgcgctcacg gttgccacga tctctccgtt
     1681 ctcttccgcg acgaacctct tcccgtaaac ctttctgaag tagaaatagt cgttcgatat
     1741 gagcttcacc tggtccttta tcaggaagta gggcacccag gccggatagt ccttgtaaac
     1801 cttccacgag agccttaccg atttttcgat gtcttctttg gaggtcatct ctctgactga
     1861 atatctttca ggctccggcg cgcccggggc gaacggcctc gctatgatgc cgaattccct
     1921 gccgagcttt tcggttactt cgagcgacct gtccaggtcc tcacgcgtat gcgttgccat
     1981 gacgctgaac ctgaacctct cctgcccctt gggcacagcg gggtgaggta tcgcatttac
     2041 gtatatcccc tcctcgtgga accttctgct catcttcttc tgggtgagct cgtcgccgat
     2101 caagacgggg atgatcgccg actccgtctg tcctgtctgg aatcccagag atttcaggtt
     2161 ttctttcatg tacttgacgt tcctccagag gttttcgtgg agggacttgt ccgactgcat
     2221 cacttcgatc ccggctaata ccgaggctgc gacggatggg gggaaattgg tcgagaagaa
     2281 aaacgacctc gtgtagtatc tcaggtaatt aacgacctct ttggtcgagg cgatgaagcc
     2341 cccgataccg cccagggact tgctgagcgt gcccatcact atgtccacct cgccgggcct
     2401 cattttgaaa tggctcgggg tcccttttcc cctgtcccct ataacccccg ttgcatgagc
     2461 ttcgtccacc attaccttcg ccccgtacct gtgggccgtc tccgtgatct ggcggagcgg
     2521 ggcgatgtcg ccgtccatgc tgtatacgcc gtcaactatg acgatctttc ccttgaagtt
     2581 gtctgtattt cttttcagca cgctttcgag gctccggacg tccgagtgct tgaacgtcct
     2641 gaaggagccc gaggacagca tgcagccgtc tataatgctc gcgtgggcga gccggtcgat
     2701 gaccgcgagg tcttcgttct tgacgagcgc ctgtatgcat ccgaggttcg taacgtatcc
     2761 gctcggaaaa attatcgcgt cctcggcgcc cttaagctcg gcgagcttca cttcgagttc
     2821 cctgtgtatg tcgaatgtcc cggcaaggag cggtacggag cccgcgcccg cgccgtattg
     2881 atctatcttc tccttggcga ttttcattac agtcggatgg gcggttaggc cgaggtagtt
     2941 gttgctgccc atcatgatca tctctttctt cttcccggta aaatcgtcgt cgacgatcac
     3001 cctgtgcgcg gaaggggacg cgagcggtct cctgtaacgg atgtacccct tcgactccat
     3061 gtccagcttg tattgccaga aaacctccgt tttcttaaac aggtcctcgt cgggaatgtc
     3121 cgcgaagtcc gataaatagt agtcgtcgga attaagggcg tgtttgctct tctctatgta
     3181 gctctctaat tttgccctgt ccgaatcgta tatgtttatg aattcgaccc cgataccgtg
     3241 ctcgccggtg ccgttcgttt cgtgcctctt aacttcgcaa tacgccttga cgggatgcga
     3301 gttggacaga ttacattcga gaagcagctt cgttcccggc tcgtaatcgt cctcggtctg
     3361 tatgaaaagc ccctttgagg agaggtcctt ggcgaggccg atcatgttgc ctgaattttt
     3421 ggaatagact tgaaaattta cgggtattct ttcgtgtttc ctgcggtctc cagactccct
     3481 gattactatc cccatgatcg ataccccctc cgggctattt tagcattatt ctttaacata
     3541 gttaagataa ttgtttatag cttgactata ataatcctcg attattagaa ttattgcaag
     3601 tatgcaatct tcggcaaccc aaccgccata ggcgcggata aagcgccctt aggcttgagg
     3661 ctttgcgtcc tgacctttcg gacagtttgc catttcggaa ggatactgac cctatttgca
     3721 atattagtgc aatttgcttt ttgtttcaat aggttatatt taacggatat gattttattg
     3781 aaagacctgt tcaagcacat tcaaatgcgg cgccgggccc ggaatcgtga tttttatagg
     3841 tattacttaa ccggcggccg tacagattat gtttgattta atcaattaaa aaccccaaaa
     3901 atattttacc cggattttta gagccttaaa aaaaacttcg gacgctctca gtgggccgat
     3961 aacaagcggc ggatttggct ccaatgtttc ggaagatacg gaacaaggaa tgaaatggat
     4021 agatctggca gttgcttttt tggctttaga tttttgcgag cctcgacatg agcaggtggt
     4081 cggcgagcac gaggttgacc atcgcttcgg caataggcac cgctctcgga catagacacg
     4141 ggtcgtgtct ccccttcacc ttcagctcta cggggtttcc gtacttatct accgtatgct
     4201 gaacctttga tattgaagat gtcggcttga tagctattct cacgacgagg tcttcgccgt
     4261 tcgtgattcc gccgaggagt ccgcctgcgt tattagtctt gaacctcagc tttccggagc
     4321 ggtccttata catgacgtcg ttcacttgag agcctttcct cttggacgcc tcgaaaccca
     4381 tcccgacctc gaatcccctg atcccgccga ggctcatcag cgctgcggca aggtccgcgt
     4441 cgagtttgtt gaataccggg cttccgagac cgggcggaag accggtggat acaacctcga
     4501 ctgtgccccc gatcgaatcc ccttccttcc tcaccttttc tataagctca atcatctcgg
     4561 cggcctttcc ggcgtccggg catcttacta tgttcctctc tatctcgtcc gggtcgactt
     4621 tacgtgcgac aatcctcccc acctgtctta cgaatccgta tgtcctgatg ccgctcaggc
     4681 tcagtatctt tttggcgatg gctcctgccg ccacccgtcc cacggtctcc cgcgcagatg
     4741 accttcctcc tcccctgtag tcccttatcc cgtatttggc gtcgtaggtg taatccgcgt
     4801 gtcccggcct gtaggtgtcc tttatatctt cgtacgactt cgatatgacg tcctcgttcc
     4861 tgaccatgag agatatggga gtgccgagcg tcttcccctc gaatacgccc gagagtatct
     4921 ctacgctgtc ggattctttc ctctgcgttg ttatcctgct ctgtccgggg cgcctgcggt
     4981 cgagctccga ctgtatatct tctttcgata tctcgagacc ggccgggcag ccgtcgacga
     5041 cgacgcccag agcctccccg tgggattcgc cccatgtcgt tatacgaaag agttgtccga
     5101 acgaattacc cggcatgatg gaagtttata ttaacgggat aatccctaca aacaagggca
     5161 ggggcgcaaa ttcagagata gccgttttcc ctgaaccagt tcaccgtgct ctttatcgtt
     5221 tcttcgagcg gcctcggcga gaacccgagc tccctcacgg ccttagaatt atcgaaatac
     5281 cagtagtgct tcgagccttt gatggagtct atgtccatcg ccacgtagtt aggggccgat
     5341 atgccgaata tccgctcggc tatgaaagcg cccaggtaag cgagagggta gggtatcttc
     5401 atcttcggcc tcggtatccc ggtcaccttc tcgaggacct cgaagagctc ctttattgta
     5461 atattcctgt tgctgagtat gtaacgctcc ccgagcttcc ccttctcggc cgcgagcagg
     5521 tgtcccgcgg cgacgtcctc ggcgtcgacc agatttatgc ctccgtccgt atagcccggc
     5581 agcctcctct tgcagtacca gagtatgatg cccgaggtcg agacgtaaac gtctcccgca
     5641 ccgattacta tcgaagggtt gactatcacg aggggcaggc ccttcccgaa aaatttaagc
     5701 gcctcctgct ccgcgtaata tttcgatttt atatactgta tgtcggtccc ggacgggtcg
     5761 aacggggtct cttcgtccgc gggctccatc cccggcttta atcctatcgc agcgacgctg
     5821 ctcgtataaa cagctttctt cacgcccgct tccatcgcgg ccgtgaatac gttattcgtg
     5881 cccccgacgt ttacctcctc catcttcttg taatcggcct gcttgaaaga aacgatgccc
     5941 gccgtgtggt agagcgtatc gcagccttcg agcgccttcc tcacggattc gatgtctgtg
     6001 acgtcgccgt aaaccctctc cgcctttata tcgtcgatgt ttatcgtttt actggtcttc
     6061 ctgacgagga gctttacctc atcccccctc tcagcgagct tcctcgcaat gtgagagccc
     6121 agaaaacccg tggccccggt aacgagtgtt gccatgaaaa aacctcctga cgccttagaa
     6181 agactcatat tacaacagct gctcctgaaa aacaatggac gcagcagccc cgcggccgct
     6241 attgcccagc ttgttgataa aacgtatgtc cggacgttga gaacctctat tatcggggta
     6301 tattgtttac ggccgggctt gagttgccgg cgcctgatta agagaggaca tcatggatct
     6361 ggaagagctg ctcagagaca gggaatacga tgagggcgag aaacgcctcg aaagggcttt
     6421 ggtcatcgcg gagagggagt gggagaaggt gaggctctcc ttcggtgaat gcggcgatat
     6481 aggcggcttc gacaaaaaag acttcatgat cgggatcatc aaagaggacg taataatcag
     6541 acagcctctg gtaagcccca ccaagtccgt ctccgtttac gcgcctacgt tttatccgat
     6601 gtacttcata aacaacctcc tcgctatgga ggagaagttc gaagatcgag ggtactcgac
     6661 ttccgaagcg ctctacgcgt acatcgagct tgcgaccagg gcttccgaga agctggggct
     6721 cgaaggcacg ttcgccatgg ccttcggagc cgggtacggg agcgtccgca ccggatgggt
     6781 cgcggaaaag gggttccccg tcgagaggga gattttcttc aatatgttct ttgaaagtaa
     6841 gaagaaggat
//
LOCUS       DASSJH010000283         4677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1038230, whole genome shotgun sequence.
ACCESSION   DASSJH010000283 DASSJH010000000
VERSION     DASSJH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038230"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFJ67_06085"
     CDS             complement(<1..251)
                     /locus_tag="VFJ67_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289992.1"
                     /translation="MSIKHIKRELLQCDHADENGERCDREGNRDAIKSCGVCGKDICI
                     THYELVTVTLQSTRDHFTYYFCPIHTDEFLDTLAKKYGDT"
     gene            452..1420
                     /locus_tag="VFJ67_06090"
     CDS             452..1420
                     /locus_tag="VFJ67_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7289993.1"
                     /translation="MITPQESLLGAIKYFQTRFTGRPKLVNLEITKLCNAKCDFCDYW
                     QTRHEERIYDYRPVIKLINPLVVVITGGEPMLRKDLPDIVKQVKESSFFVFTSMVTKG
                     DLLTVDKAEELYDAGINQIAISLDFPGKTHDTYRGVPGLWDHLSELLPEMGKRFPKGK
                     VTLNTIIMEDNIDHIPELAARAREWGVGISFSSYSVMKTNNETHFVHPKELERVKKLV
                     EELIMLRKKWKGTILSTEYYLKEIPRYFEKGGIPGCTAGISHIQVTPSGHLKRCSEMP
                     ITTHYSEYTPKLYDKTECTACWYACRGETQTPASIRRALEYMGVWV"
     gene            complement(1519..2955)
                     /gene="cysS"
                     /locus_tag="VFJ67_06095"
     CDS             complement(1519..2955)
                     /gene="cysS"
                     /locus_tag="VFJ67_06095"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7289994.1"
                     /translation="MEKKVTLYNTLTQKSEELVPHEKNKIKMYVCGPTVYDSAHLGHA
                     RAAVTFDVIERFLSHVGYDVTYVRNFTDIDDKIINRANETGIPANEISEKYIQEYRED
                     MASIGVKRPTVEPKVTEHVPEIIDLIERIIDNGYAYQSGDDVFFSVKKFKGYGKLSKR
                     DPDDMLAGTRIDINEKKEDPLDFALWKGAKPGEPFWESPWGKGRPGWHIECSTMSMKY
                     LGPSFDIHGGGKDLIFPHHENEIAQSEAATGKEFAKYWVHNGLIQINREKMSKSVGNI
                     LNVRDAVQMWNREAIRLFFLSHHYLNPADFSDTTMGESEAALERLYITLKRAKDQRKN
                     GSADDPELAGAVASFGRKWVETMCDDFNTADAIGNLFELTRALNRSIDSKGWTPALEG
                     ALKEIDNFGKALGILEYEPEEYLQTIKLEKKSAGITEEEIHELINERNAARKEKNWKR
                     ADEIREELSSRGILLEDKAEGTIWRVKG"
     gene            complement(3002..3805)
                     /locus_tag="VFJ67_06100"
     CDS             complement(3002..3805)
                     /locus_tag="VFJ67_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7289995.1"
                     /translation="MGHGLENSWALILGASSGFGEAAALELASHGMNVFGVHLDRKST
                     MPKVEEIKVKIKAKGREAAFFNINAADPEQRSETLGEIEQILDEHKNPSGIKVLIHSL
                     AFGTLKPYIAESEKDRVSNKNMDMTLDVMAHSLVYWAQEIVARKMMIKGGRIFAMTSA
                     GSHRVWPTYGPVSASKAALESHIRQLSVELAKHGITANAIQAGVTETPALSKIPGNEE
                     IIRHALAVNPAGRLTTVTDVAQAIAALSVPGTHWLTGNVIKVDGGEDLI"
     gene            4162..4653
                     /locus_tag="VFJ67_06105"
     CDS             4162..4653
                     /locus_tag="VFJ67_06105"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7289996.1"
                     /translation="MSQIYCSSVVTRIGQVFVLSSEKGVTRIIFGVKEFRNYLDGLGG
                     AQIVEGGPAAEMAHEIELYFDGRLTEFSTPIDLTEGTPFQRSVWEKLLDIPYGKTATY
                     KDVAEGVGKPGASRAVGNAVGANPIPIVIPCHRVLASNGLGGYSGGINIKKDLLRVEG
                     TLS"
BASE COUNT          926 a         1278 c         1326 g         1147 t
ORIGIN      
        1 gtatcaccgt actttttggc gagtgtgtcg aggaactcgt ccgtatgaat ggggcagaaa
       61 tagtaggtga agtggtcgcg cgtgctctgg agcgtgactg tcaccaactc gtagtgcgtt
      121 atacatatat ctttcccgca cacgccgcac gactttatgg cgtccctgtt tccttctctg
      181 tcgcacctct ccccgttctc gtccgcatgg tcgcactgga ggagctctct ctttatgtgt
      241 ttaatcgaca cctgtcaccc catgtatata caagtccccg tccctgttcc ctttatctat
      301 taaaataaat cgcgggagat aagtcaatat gttttattcc atccgattcc gatcacggct
      361 cgcttaaccc gggcttagta cgaatccgat atccggcggt catctggcgt gtccgcttga
      421 aaactcgcct cgtgttatgt ataaatctat aatgattaca ccacaggaaa gcctcctcgg
      481 ggcgattaaa tatttccaga cccggttcac cgggcgcccc aaactcgtca atctcgagat
      541 cacgaagtta tgtaacgcga agtgcgattt ctgcgactac tggcagacga ggcacgagga
      601 gcgcatatac gattacaggc ccgtcataaa actgataaac cccctcgtcg tggtaataac
      661 gggcggtgaa cctatgctca gaaaggacct ccccgacata gtgaagcagg tgaaggaaag
      721 ctccttcttc gtcttcacgt cgatggtgac gaagggcgac ctgctcacag tcgataaggc
      781 ggaggagctt tacgacgcgg ggataaacca gatcgccata tcgctcgact tcccggggaa
      841 gacgcacgac acatacagag gagtgccggg actctgggac catctgtccg aattacttcc
      901 cgaaatgggt aagaggttcc cgaaggggaa ggtgacgctc aacacgatca tcatggagga
      961 caacatcgac cacatccccg agctcgctgc cagggcgagg gagtggggcg ttggtatatc
     1021 gttcagctcg tactcggtga tgaagacaaa caacgaaaca catttcgtcc atccgaagga
     1081 gctcgaaagg gttaagaagc tcgtggaaga gctcatcatg ctaaggaaga agtggaaggg
     1141 gacgatacta tcgaccgagt attacctgaa ggagataccg agatatttcg agaagggcgg
     1201 gataccggga tgcacggccg ggataagcca catacaggtt acgccgtccg ggcatttgaa
     1261 acgctgctcc gagatgccta ttaccacgca ttattccgaa tacacgccga agctttacga
     1321 caagacggaa tgtaccgcgt gctggtacgc ctgccgcggt gagacgcaga cgcccgccag
     1381 catcagacgc gcgctcgagt acatgggcgt ctgggtgtga gcttgtcggg atgtggcttc
     1441 ctacttccgt aggaacgcca ttcccttacg tgtgtcgcgg ccggaaaccg cccctacaat
     1501 tattgtgaag aagcttgctt atcccttaac ccgccagatg gtgccttcgg ccttgtcttc
     1561 gagaagtatg ccgcgggagc tgagctcttc cctaatctcg tctgcgcgtt tccagttttt
     1621 ttccttccta gcggcgttcc tttcgttaat aagctcgtgg atctcttctt ccgtgattcc
     1681 tgcggatttt ttctcgagct ttatagtctg gagatattct tcgggctcgt attcgagtat
     1741 gccgagggcc ttgccgaaat tatcgatttc tttgagcgcc ccttcgaggg cgggggtcca
     1801 tccctttgag tcaatagacc tgttcaaggc gcgtgtgagc tcgaacaggt tccctatggc
     1861 gtcggcggtg ttgaagtcat cgcacatagt ctcgacccat ttccggccga aagaggcgac
     1921 ggcgcccgcc agctccgggt cgtcggcgct cccgttcttg cgctggtcct tcgcccgctt
     1981 gagcgtgatg tagagcctct cgagagcggc ttccgattcg cccatcgtcg tgtccgagaa
     2041 gtcagccggg ttgagataat ggtgcgagag gaagaagagc ctgatggcct ccctgttcca
     2101 catttggacg gcgtccctca cattcaaaat gtttccgacg gatttggaca tcttctcgcg
     2161 gtttatctgt atgaggccgt tgtgcaccca gtacttcgca aactcttttc cggtggccgc
     2221 ttccgactgg gcgatctcgt tctcgtggtg ggggaaaatg aggtccttcc cgccaccgtg
     2281 tatgtcgaag ctcggcccga ggtatttcat gctcattgtc gagcactcga tgtgccaacc
     2341 cgggcgtccc ttgccccacg ggctttccca gaagggctct cccggcttgg cccctttcca
     2401 gagcgcgaag tcgagcgggt cttctttctt ctcgtttata tctatcctcg tgccggcgag
     2461 catgtcgtcg gggtcgcgct tcgagagctt gccgtagccc ttgaacttct tcacggagaa
     2521 aaagacgtcg tcgcctgact gataggcgta gccgttatcg attatccttt ctatgaggtc
     2581 tatgatttcg gggacgtgct cggtgacctt gggctcgacg gtcggcctct tcacgcctat
     2641 agaggccatg tcctccctgt actcctgaat gtacttctcc gatatttcgt ttgccgggat
     2701 gccggtctcg ttcgctcggt tgataatctt gtcgtcgatg tcggtgaagt tcctgacgta
     2761 cgtgacatcg taacccacgt gagagaggaa cctctcgatg acgtcgaagg tgacggcagc
     2821 cctcgcgtgt ccgaggtggg cggagtcgta cacggtcggg ccgcagacgt acatctttat
     2881 tttatttttc tcgtgcggga cgagctcttc gcttttttgt gtgagcgtgt tgtagagggt
     2941 tactttcttt tccattattt atttccggat aaggggcgtg agcggatgct ccggtatgac
     3001 gctaaatcag gtcctcccct ccgtccacct tgattacgtt ccccgtgagc cagtgagtgc
     3061 ccgggacgga cagggctgcg atcgcctggg cgacgtcggt cactgtcgtc agccggcccg
     3121 cggggttgac cgcaagggca tgtcttatta tttcctcgtt gcccggtatc ttgctcaggg
     3181 cgggggtctc ggtcacgccg gcctgtatcg cgttcgccgt gatcccgtgt ttggcgagct
     3241 cgacggagag ctggcgtatg tgcgattcga gggccgcctt cgacgccgac accgggccgt
     3301 acgttggcca caccctgtgg ctgccggcgc tcgtcatggc gaatatcctg ccgcccttta
     3361 tcatcatctt cctcgcgact atttcctgcg cccagtaaac gagcgagtgc gccatgacgt
     3421 cgagcgtcat gtccatgttc ttgttagata ccctgtcttt ttccgattcc gcgatgtaag
     3481 gcttgagcgt gccgaatgcg agggagtgaa tgagcacctt tatcccggaa gggtttttgt
     3541 gctcgtccag tatctgctcg atttctccca gagtctcgga gcgctgctcc gggtccgccg
     3601 cgtttatgtt gaagaaggcc gcctctctcc ccttcgcctt tatctttacc tttatctcct
     3661 cgaccttcgg catggtggat ttcctgtcga ggtggacgcc gaagacgttc atcccgtgag
     3721 acgcgagctc gagcgcggcc gcttcgccga acccgctcga cgccccgaga attagtgccc
     3781 agctgttctc aagtccgtgt cccatgccct gtaaatcccc cttgtcggtt atttaatcct
     3841 ttaaatttgc agtagagata ttacgtactc tggggggaat ttcaaggcgg cggggccgca
     3901 ggcgtgcagg agcataaccc gttcaatcct tcccagctac ggctcgggtc ttcgccgaga
     3961 cgagcgacaa gcttccggct tcgccatcct cctatgctaa agcttcggag gataaaaagc
     4021 ttcgccggac aagcacgatg aacgggttgg ggattggtgc cggctatccg aaagagttac
     4081 aatagaatag tatacgagtg caggttctgc gccttgacat ggattatctg cgcgtggaaa
     4141 ctgattcggc ggggggcatt catgagtcaa atctattgtt catcggtcgt tacgcgtata
     4201 ggtcaggtgt tcgtgctctc tagcgaaaag ggcgttacga gaataatatt cggtgtgaag
     4261 gagttcagga attatctcga cgggctaggc ggggctcaga tagtggaggg agggcccgct
     4321 gccgagatgg cccacgaaat cgagctctac ttcgacggca ggctcacaga gttcagtacc
     4381 ccgatagacc tcacggaagg cactccgttc cagaggtcgg tctgggaaaa actcctcgac
     4441 attccgtacg ggaagaccgc cacgtataag gacgtggccg agggcgtagg caagccgggg
     4501 gcgtcgaggg cagtggggaa cgcggtcggg gcgaacccta tacctatcgt aataccgtgc
     4561 cacagggtgc tcgcgtctaa cggactcggc ggttattcgg gcggcatcaa tatcaaaaag
     4621 gacctcctca gggtggaggg gacgctctct taggtgaaaa aggggaccgc aaagctc
//
LOCUS       DASSJH010000284         3519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_285847, whole genome shotgun sequence.
ACCESSION   DASSJH010000284 DASSJH010000000
VERSION     DASSJH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285847"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VFJ67_06110"
     CDS             complement(<1..923)
                     /locus_tag="VFJ67_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289997.1"
                     /translation="MRERQFYLRAAGIVLLAVALTGCETGGPGLSVKGRVVAGGVPVS
                     GSSVTLYAAGTIISETLGYSTTDKEGNFSIKFNKPSESGIIYAVARGGKPEGSVSANE
                     ALAFLTVIGEVNDRPGSVTLNEITTIASVWTYAQFLNGSSIVGNGTGIYNAAANVRNI
                     ANIETGGPGPVIQNAVNGSRTNALPEVNTLGNIVHTCASLSHPDACASLFSASAPPGW
                     NVPEDTLSALHNIALNPWWNVTGIFSLAPPAAPYFPALNTAPAAWTIALKFTGGGLDA
                     PGAVSIDEDGNVWATNDLLYGPQPELSACGDG"
     gene            complement(1262..1468)
                     /locus_tag="VFJ67_06115"
     CDS             complement(1262..1468)
                     /locus_tag="VFJ67_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289998.1"
                     /translation="MTRESTRISLLIDSDIRDSLDRIASAYGKNMSTVIGEAIEQYID
                     VHERHASLTQIEADASEEGDFATD"
     gene            1603..2184
                     /locus_tag="VFJ67_06120"
     CDS             1603..2184
                     /locus_tag="VFJ67_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7289999.1"
                     /translation="MSSDMAEAEGTRLPRAYRRNSKFKGLSMAEEFDNDIREIARSLQ
                     AGEASPGGAPALTGRDKEFLAAIEDLYVHFRRAITIFNDVSPGMRLSLQRLTPGFLDL
                     FLSIPGRRGGFSVFSPSRIVILFDEDPNVLTVIGKRRRTEKDGEGKLSPAIQLIKINF
                     RMQDNAITYRDNAGGPLELHSLVTLIIKWVSAA"
     gene            complement(2189..2341)
                     /locus_tag="VFJ67_06125"
     CDS             complement(2189..2341)
                     /locus_tag="VFJ67_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290000.1"
                     /translation="MSEHEKKEGHKPLFELSEREKSGALYILGVAILIFSIVFLASLT
                     HVVLNP"
     gene            complement(2621..>3519)
                     /locus_tag="VFJ67_06130"
     CDS             complement(2621..>3519)
                     /locus_tag="VFJ67_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7290001.1"
                     /translation="DGSDESEEALNYAIFLARIFRSEITGVYVIEMHPRLLYDYARDP
                     DSELYSWVEQTAENHKERLISEAEISGIQGIAFRTTVSIGDPSEEIVKLARRKKADLI
                     VLGVRGLGLIDKMLVGSTTLKVLRKSRIPVLSVRKRGSGEAIELRNILVPLDIYDKED
                     SALDYAVDLAQAIKTNISVVYAYRLFDYAAIEEGKTPSGINELGEEALKFFSSELAAR
                     VEGPRERLKRDGMEAGSGITTDLIEGKTPAMRIVEYADSKNTDLIVINTHGRKGIKKI
                     MMGSVTDKVVQESPCPVLALKP"
BASE COUNT          709 a         1004 c          972 g          834 t
ORIGIN      
        1 ccgtcgccgc aggcggagag ctccggctgc gggccgtaaa gaaggtcgtt ggtcgcccaa
       61 acgttcccgt cctcgtctat cgatacggcg ccgggagcgt cgagaccgcc gcccgtgaat
      121 ttgagcgcga tcgtccatgc cgcgggcgct gtgttgagcg cggggaaata aggcgccgcc
      181 ggcggcgcga gcgagaatat tcccgttacg ttccaccacg ggttaagcgc gatattgtga
      241 agggccgaga gcgtgtcttc cgggacgttc catccggggg gcgccgaggc cgagaagagg
      301 ctcgcgcagg cgtccgggtg cgatagcgag gcgcatgtgt ggacgatatt cccgagcgta
      361 ttcacctccg gaagcgcgtt cgtccgtgag ccgttgaccg cgttctgtat taccggtccc
      421 ggccctcctg tctcgatgtt ggctatattc ctgacgttcg ccgcggcgtt atatatgccc
      481 gttccgttcc cgacaatcga gctcccgttc aggaactgag cgtacgtcca gaccgaggcg
      541 atggtcgtga tctcgttcag cgtaacggag cccggcctgt cgttcacctc gccgatcacg
      601 gtgaggaagg cgagcgcttc gttggcggag acgctgccct caggtttccc tccgcgcgcc
      661 acggcgtata tgatcccgct ctcggagggc ttgttgaatt taatgctgaa attcccttct
      721 ttgtcagttg ttgaatagcc cagggtttct gagattatgg tcccggccgc gtataacgtt
      781 acgctcgatc cggatacggg cacgccgccc gcgacgaccc tgcccttgac actaaggccg
      841 ggcccgccgg tctcgcaccc cgttaacgcc acggcgagca gaacaatccc ggcggctcta
      901 aggtaaaact gtctctctct caatcgggtt gttccccccc cggagtacat aggcaggaat
      961 ctaacattac ccgatccgcc cgttatttca acccacgcac aaagtgcgtt ctatgattct
     1021 actcaggctc ctccgctcgg ccaataacca acccgttcgc cttgcctgtt cggcgtaccc
     1081 tcttgcgaag cctaatgcct gtcgatgggt tgaacggctt tggtacagtg ttcgctatgc
     1141 ggatgagtct gggaagaacc gtatatgagc acagattctg tgccacaaat tgtgtgccac
     1201 ggacgcagct ctcctgtatg ttttccatgt gtgagccgga tggccgggaa agcgtgcggc
     1261 ctcagtcggt cgcgaagtct ccctcttcgg atgcgtcggc ctcgatctgc gtgaggcttg
     1321 cgtgccgctc gtggacgtct atgtactgct cgatcgcttc gcctatgacg gtgctcatgt
     1381 tcttgccgta agccgaggca atcctgtcga gcgaatcacg tatgtccgag tcgatcagta
     1441 acgatattct cgtggattcc cttgtcataa tctctgctcc tctgtttaat atcctagcac
     1501 acccggccgc gcgaaaacag tctaaaaaat ccgggagccc gcggtgatga gacgagattg
     1561 aatatccgca gcgctttacc tcccgctcaa ggcgattata atttgtcatc cgatatggcg
     1621 gaggccgaag ggacgaggct tccgcgcgct tacaggcgga attcaaaatt caaagggttg
     1681 tcaatggcgg aagagttcga caatgatata agggagatcg cgaggagctt gcaggcgggt
     1741 gaggcttcgc ccggcggggc tcctgcgctt acgggcagag ataaggagtt cctcgccgcg
     1801 atagaggacc tttacgtgca cttccgccgg gcgataacta tctttaacga cgtatcgccg
     1861 ggcatgaggc tatcgctcca gaggctcacg cccggtttcc tcgacctctt cctctcgata
     1921 ccgggcagga ggggcggctt ctccgtattc tccccgtcga ggatagtgat cctgttcgac
     1981 gaagacccca atgtgctcac agtgatagga aagaggagga ggaccgaaaa ggacggcgaa
     2041 ggcaagctct cgccggctat acagctcatc aaaattaatt tcaggatgca ggataatgcg
     2101 ataacttaca gggacaacgc gggggggccg ctcgagctcc acagcctcgt aacgcttatc
     2161 ataaagtggg tatcggctgc ctgaaagctc aggggttgag cactacgtgg gtgagtgacg
     2221 cgaggaatac gatggagaat atcagtatcg ccacgcccag gatgtaaagc gctcccgact
     2281 tctcccgctc gctcagctcg aataacggct tatgcccttc ttttttctcg tgttcgctca
     2341 tgaccgcaac attataacgg ctaattcacc gttgtaaaga atacacagac taaagaaccc
     2401 gttccgggtc cggagcgggc cggatagcgg tgatgaaatc gtaacgactc ggttctcttc
     2461 gggatttcat tccggatttc agcactttac gattttttag gtgctcaagc gcgggcgtta
     2521 cgcctagccg gaaatagtaa tccggcgaca gccggcatag gcatacataa acacaggcgt
     2581 gaaatagaga cccgccgggg attttgcggt tatgcgcggc ttatggctta agcgccagca
     2641 cggggcaggg ggattcctga acgaccttat ctgtcacgct gcccatcata atctttttaa
     2701 tcccctttct tccgtgtgtg ttaattacga ttaaatccgt gtttttgctg tccgcatact
     2761 cgactattct catcgccggg gtttttccct cgatcaaatc cgtggtaatc cccgatccgg
     2821 cttccatgcc gtctctctta agcctttccc tcgggccctc gacacgcgcc gcaagctcgg
     2881 aggaaaaaaa cttcagcgcc tcctcaccca actcgttgat tccggacggg gttttccctt
     2941 cttctatggc ggcgtaatca aacagcctgt atgcatacac gaccgagata tttgttttta
     3001 tcgcctgtgc taaatctact gcatagtcga gggctgagtc ttccttatcg tatatgtcca
     3061 gagggacgag aatattacgc agctctatcg cttcaccgct tccccgcttc ctcacggaaa
     3121 ggacgggtat gcgcgatttt ctcaaaacct tcagggtagt gctgccgacc agcatcttgt
     3181 ctatgaggcc gagtccccgt accccgagca caatcaggtc ggctttcttt cggcgagcga
     3241 gctttacgat ctcctcactg gggtcgccta tcgatacggt agtccggaaa gcgatcccct
     3301 gaatgcctga tatctccgct tcggatatga gtctttcctt gtgattttcc gcggtttgct
     3361 ccacccagct gtagagctcg ctgtcagggt ccctggcgta gtcgtataag agcctgggat
     3421 gcatttcgat aacatatact ccggttatct cggagcggaa aatacgcgcg aggaagatcg
     3481 catagttaag tgcttcctcc gattcatccg agccgtcgc
//
LOCUS       DASSJH010000285         7605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_827999, whole genome shotgun sequence.
ACCESSION   DASSJH010000285 DASSJH010000000
VERSION     DASSJH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827999"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFJ67_06135"
     CDS             <1..455
                     /locus_tag="VFJ67_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HET7290002.1"
                     /translation="YLIGMMKVLGVHSYGRMIPNMPSRDDYGHQFKLDTISRYKFTIA
                     FENAIAADYVTEKFYDPLIAGSVPVYLGAPNVSDFAPGENCFINASVWDDPESLGRYL
                     IELSHDDELYAGYFEWKNKPFLPKFRELLELQKINSFVRLCRKVGELT"
     gene            623..697
                     /locus_tag="VFJ67_06140"
     tRNA            623..697
                     /locus_tag="VFJ67_06140"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:655..657,aa:Val,seq:gac)
     gene            714..2648
                     /gene="thrS"
                     /locus_tag="VFJ67_06145"
     CDS             714..2648
                     /gene="thrS"
                     /locus_tag="VFJ67_06145"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7290003.1"
                     /translation="MKEVYINLPDGAQRTFPEGTTVGEIIETIGIRNGTIGARVNGTL
                     VDFWHEIYSDSELEPVKAGSEEGLALIRHTAAHVMAEAVQSLFPEAKVTIGPVIENGF
                     YYDFEYPKGFTPEDLEKIEEKMKEIVGEKKPLVRKKVSREKAIDIFSSEGEKYKVEIV
                     NGLPPDQQITIYEQGGWHDLCRGPHAPSTDYVKAFKLLSIAGAYWRGDEKNPMLQRIY
                     GTAFWDRKALKEYLDRLEEARKRDHRKVGRELDLFSIQEETGAGLVLWHPKGSRIRRV
                     IEDFWRDQHERYDYELLYTPHIAKIDLWKTSGHLEFYNENMYPRMEVENTEFQIKPMN
                     CPFHIMIYKTGIRSYRDLPLRWAEIGTVYRYERSGVLHGLMRVRGFSQDDAHIFCRPD
                     QMEDEVLGVLDLTILFLKTFGFDDYEVYLSTRPEKYVGNVEGWDRATEALRRSLEKKG
                     LKYEVDEGGGAFYGPKIDLKIKDVLGRAWQCSTVQVDFNLPERFEMTYVGEDNARHRP
                     IMIHRAIFGSMERFFGVLIEHYGGAFPLWLSPVQVRIATVGDEQAGYADEIYSTLRAG
                     KIRVENDKRNEKLGLKVREAQLAKIPYLLVVGKNEAESGTVAPRKLGGKNLPPMTIDE
                     FLHSIAIELDPETWREVERQ"
     gene            2645..3196
                     /gene="infC"
                     /locus_tag="VFJ67_06150"
     CDS             2645..3196
                     /gene="infC"
                     /locus_tag="VFJ67_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET7290004.1"
                     /translation="MAKGFKTKEAEVRINQRIKAREVRLISDTGEQLGVVPIREALQM
                     SEERGVDLVEVAPNANPPVCRLMDYGKYKYELKKQANAKKQKVQTIKELKFRPNIGDH
                     DLDVKINRIREFLEDDNKTKIRIFFRGREIVHPELGRVLANRIIERVADIGGVDMPPR
                     LEGKNLIMVLSPKKSTQTGRENA"
     gene            3189..3392
                     /gene="rpmI"
                     /locus_tag="VFJ67_06155"
     CDS             3189..3392
                     /gene="rpmI"
                     /locus_tag="VFJ67_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET7290005.1"
                     /translation="MPKQKVKTNRSAAKRFKVTGSGKVKRWSGGKSHSNAKKGRKRMR
                     RLVASKIEEGGEARRIKSYIPYK"
     gene            3467..3820
                     /gene="rplT"
                     /locus_tag="VFJ67_06160"
     CDS             3467..3820
                     /gene="rplT"
                     /locus_tag="VFJ67_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET7290006.1"
                     /translation="MRIKRGVASRKRRKRVLKQAKGFWGRRKSNIRRAKETVIRAMAY
                     AFRHRRLKKRDFRKLWIARINAAVRPYGLSYSAFMGSLKKAGIELDRKTLSEMAIREP
                     DSFKAVVDAAKTGLN"
     gene            complement(4251..4907)
                     /locus_tag="VFJ67_06165"
     CDS             complement(4251..4907)
                     /locus_tag="VFJ67_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HET7290007.1"
                     /translation="MTKSARIKKFYEELHKKFGPQGWWPGETALECILGAILTQNTSW
                     KNVEKAIGNLKRENLISVEDLNLIKADELAELIRPSGYYNQKAAKIKNFTSFLIDEFS
                     GSLDRMFSVDKSILREKLLSLKGIGPETADSILLYAGAVPVFVVDAYTWRVLHRHGLV
                     PEQTSYGEIQELFTDSLQEDPVIFNEYHALLVKLGKEHCRKREPLCAGCPLEYDPHGV
                     "
     gene            complement(4972..5754)
                     /locus_tag="VFJ67_06170"
     CDS             complement(4972..5754)
                     /locus_tag="VFJ67_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7290008.1"
                     /translation="MVLAGAIAVACYKAPVTGRSQFILLSQSEENQMGVAAFQEVLKE
                     EKVSTNAQYNEAVTRVGTRIVGVADTPQYNWDFKVFNDDETINAFALPGGKVGVYTGI
                     LSVAQNDAGLATVMAHEVAHVAARHGGERVSTDVLAQIGAVGVSAALGGSDPWVSEAI
                     MQAYGIGVTVGGILPFSRTQEAEADHIGLVYMAKAGYDPREAIAFWERMDAAVKGKPQ
                     PPEFLSTHPGYGTRINNLKQWLPQALPYYEQAQKAPNNAITK"
     gene            complement(5974..7158)
                     /locus_tag="VFJ67_06175"
     CDS             complement(5974..7158)
                     /locus_tag="VFJ67_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7290009.1"
                     /translation="MAVSYPFSSKISHFVPPKQWASMRVARELEKKTGQKIIHFEKGD
                     YQGPDFYTPDHVLEATVQALKDGYVRYDPGPGLPELRAAIAAEMTREGRKTKPEEVIV
                     TAGAKHALTMCLLTFLEEGDEVIFPNPGYPPDEVWAKYVRAKINHTPLTKPDWQFDLE
                     KLEKMITPQTKLLIINTPQRPNGHLVENPEEIAELCFKYPNLMVISDEIFSHVTYDKP
                     HKTISAVPGMEERTIVIDTFSKTYAMTGWRIGWAVAPVPVIDKLSIFLQDSITNVAAF
                     IQKAAYAAMAGPQDWTIEKRELLKKKRDRMVAGLNSIPGIQCDTPDGAFYAFPDITGT
                     GLTSQEFTDRLVETAGVAVVAGTAFGSQGEGYVRVTYACADEDIDEGIRRIKNADLKK
                     VA"
     gene            complement(7200..>7605)
                     /locus_tag="VFJ67_06180"
     CDS             complement(7200..>7605)
                     /locus_tag="VFJ67_06180"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290010.1"
                     /translation="SILEKETAKSDPAPVKLKKMLALIFKFYLQNLDVLRILSRDETH
                     LIREHYEFTEHWRLRRIKLYRKILEKGINEGSFRSSNTELTALIIFGLVRSVMFYYKS
                     EKSPESIADEVFSMISEGILAPGYEMSEAARM"
BASE COUNT         1815 a         1997 c         2088 g         1705 t
ORIGIN      
        1 agtatctgat aggaatgatg aaggttctcg gtgtccattc gtacgggcgg atgattccga
       61 acatgccgtc ccgcgacgat tacgggcacc agttcaaatt ggatacgatt tcgagataca
      121 agttcacaat agcattcgag aacgcgatcg ccgccgatta tgtcacggaa aaattctacg
      181 atcctttgat cgcgggctcc gtgcccgttt acctgggcgc gcccaacgtg tctgacttcg
      241 cgccgggcga aaactgtttt atcaacgcct ccgtatggga cgaccctgag tcccttggcc
      301 gttatcttat tgagctctcc catgacgacg agctttacgc cggctatttc gaatggaaga
      361 ataaaccgtt cctgccgaaa ttccgggagc ttctcgagct ccagaagatt aattcgttcg
      421 tcagactctg cagaaaggtc ggggaattga catagtattc cggaagataa gtaaatccgt
      481 cccgctcacg ccaatctgcc gctggccggg atatataggc gaatttacga ctataaatta
      541 agtgttaaga cgggatatag gcgttcccaa tcttgacagc tattatttta tataagaaaa
      601 ttaagggttc tgataatttt ttgagcgcgt agctcagggg gagagcgctt gcttgacacg
      661 caagaggtcg caggttcaaa tcctgccgtg ctcaccatgt gaaatcggaa aatatgaaag
      721 aagtatatat aaaccttccc gacggggctc aaaggacatt tcctgaaggc acgactgtag
      781 gggagataat cgaaactatc gggatccgga acgggaccat aggggccagg gtcaacggca
      841 cgctcgtgga cttctggcac gagatatatt ccgactccga gctcgagccc gtgaaggccg
      901 gctccgagga agggctcgcc ctaataaggc acacggccgc gcacgtcatg gccgaggctg
      961 tccagtccct cttccccgag gcgaaggtca ccatcggccc cgtgatagag aacgggtttt
     1021 actacgactt cgagtacccc aagggcttca ctcccgaaga cctcgagaag atcgaggaga
     1081 agatgaagga gatagtcggg gagaagaagc cgctcgtcag gaaaaaggtc tcgagggaaa
     1141 aagcgataga catattcagc tccgaaggag agaaatacaa ggtcgagatt gtgaacggcc
     1201 tccccccaga ccagcagata acgatttacg agcagggcgg ctggcacgac ctctgccggg
     1261 gcccgcacgc gccctcgacg gattacgtca aggcgttcaa gctcctgagc atagcgggcg
     1321 cctactggag gggggacgag aagaacccca tgctccagag gatatacggc acggccttct
     1381 gggacagaaa ggccctcaaa gagtacctcg acaggctcga ggaagcccgc aagcgcgacc
     1441 atagaaaggt cggcagggag ctcgacctct tctccataca ggaagagaca ggggccgggc
     1501 tcgtgctctg gcacccgaag ggctcccgca taagacgcgt catcgaggac ttctggcgcg
     1561 accagcacga gcgttacgac tacgagctcc tctatacgcc tcacatagcc aaaatcgacc
     1621 tctggaaaac gagcggacac ttggagttct acaacgagaa catgtaccca cgcatggagg
     1681 tcgagaatac cgaattccag ataaagccta tgaactgccc gttccacatc atgatctata
     1741 aaacgggcat aaggagttac agggacctgc ccctcaggtg ggccgagata gggaccgtct
     1801 accgctacga gcgctcgggc gtgctccacg ggctcatgag ggtgaggggt ttctcacagg
     1861 acgacgccca catattctgc aggcccgacc agatggagga cgaggtgctg ggggtgctcg
     1921 accttacgat cctgttctta aaaactttcg gattcgacga ttacgaggta tacctctcga
     1981 cccggcccga gaaatacgtc ggcaacgtag aaggctggga cagggctacg gaagccctca
     2041 ggcgctctct cgaaaagaaa gggctcaaat acgaggtgga cgagggcgga ggcgctttct
     2101 acggccccaa gatcgacctc aagataaagg atgtattggg cagagcttgg cagtgctcta
     2161 cagttcaggt agattttaac cttcccgaaa ggtttgaaat gacctatgtc ggggaagata
     2221 acgcgaggca caggccgatt atgattcacc gcgctatctt cggctcgatg gaaaggtttt
     2281 ttggtgtgtt gatagagcat tacggaggag ccttcccgct ctggctcagt cccgtgcagg
     2341 ttcggattgc gaccgtcggc gacgagcagg ccggctatgc ggacgagata tacagtacac
     2401 tcagggcggg gaagataagg gtcgaaaacg ataagaggaa tgaaaaactg ggacttaaag
     2461 taagagaggc gcagctcgcg aagattccct atctccttgt ggtaggaaaa aatgaagcgg
     2521 aatcagggac tgtggcgccc aggaagctgg gcggaaaaaa tttacctccc atgacgatag
     2581 acgagttcct gcacagtata gcgatcgagc ttgatcccga gacatggagg gaggttgaac
     2641 ggcaatagct aaagggttta aaacaaaaga ggccgaagtc cggataaacc agaggataaa
     2701 ggccagagaa gtaagactga tctccgatac cggggagcag ctcggggtgg ttccaataag
     2761 ggaagcgctc cagatgtccg aggaaagggg cgtcgacctg gtcgaggtcg ctccgaacgc
     2821 gaaccctccg gtatgcaggc tgatggatta cgggaaatat aaatacgagc tcaagaaaca
     2881 ggcgaacgcg aagaagcaga aagtacagac tataaaagag ttgaaatttc gccccaacat
     2941 aggtgaccac gacctcgatg tgaaaataaa ccgcatcagg gagtttttgg aggacgataa
     3001 caaaacgaag ataaggatat ttttcagggg cagggaaatt gtccacccgg agctgggaag
     3061 ggtgctcgct aacaggatta tcgaaagggt agcggatatc ggcggcgtgg acatgccgcc
     3121 aaggctcgag gggaaaaacc tcattatggt cttatcgccc aagaaaagta cccaaaccgg
     3181 gagggaaaat gcctaaacaa aaggtcaaga ctaacaggag cgccgccaag aggttcaagg
     3241 taaccgggag cggcaaagtc aagagatgga gcggcggcaa gagtcactca aacgcgaaga
     3301 agggacgtaa gcgtatgagg aggctcgtag cttccaagat cgaagagggc ggcgaggcgc
     3361 gcaggataaa gtcatacata ccttataagt aaatatccgc ccgtgtgtaa acgggtttga
     3421 ttgacggccg tcgcttatgg cggcaatata aagacgggag aaaaagatga gaataaaaag
     3481 aggggttgca tcgagaaaga ggagaaagcg cgtcctcaag caggcgaagg gtttctgggg
     3541 ccggaggaag agcaacatca ggagagccaa agagaccgtc atccgcgcaa tggcctatgc
     3601 tttcaggcac aggaggctca agaagaggga cttcaggaag ctgtggattg cgaggataaa
     3661 cgccgctgta aggccttacg gactttccta cagcgcgttc atgggctcgc ttaaaaaggc
     3721 cggcatagag ctcgacagaa agaccctttc cgaaatggca atacgcgagc ccgacagctt
     3781 caaggccgtc gtcgacgccg ccaagaccgg gcttaattaa ccgttttcct ttcccaatcc
     3841 aaccgacacg aaagaagcaa tatatctagg cccgcattca cactgaatgc gggccgcagt
     3901 cttatatcaa attccttccc ccttgaggga cgattgcaag caagcgcgca atcggacgcc
     3961 gcggaacagc ggcgcagatg caaattactt ggctgtaaat gctgcctaaa acaaaaaagg
     4021 caaggatggg ggtgtacctt accgttctgt cattcctgaa taccttctcg ggaattgttt
     4081 ctttataaac tgtatgagtg gcttccagcc gcgacaatag gatgtctttg tgaggggagt
     4141 tgagacgtgt caacgcagcg cggctatctc tcattactct ttaattcctc cctttagtaa
     4201 agggaggcca ggagggattt atctgttacc tttctttata acaaccactt tcacacccca
     4261 tgcggatcat actcgagcgg gcaccccgcg cataaaggct ccctcttcct gcagtgctcc
     4321 ttcccgagct tcacgagcag cgcgtggtat tcgttgaaaa ttaccgggtc ctcttgaagt
     4381 gaatcggtaa acaattcctg gatctcgccg tatgacgtct gctccggcac gagtccgtgc
     4441 ctgtgaagca ccctccacgt gtacgcatcc acgacgaata caggaacggc ccccgcatag
     4501 agcaagatgc tgtccgccgt ctcagggcct atgcctttaa gtgaaagcag cttctctctg
     4561 aggatcgatt tgtcaacgga gaacatcctg tcgaggctcc ccgagaattc atcgatgagg
     4621 aacgatgtga aattctttat cttcgccgct ttctgattgt agtagccgga tggccttatc
     4681 agttccgcta attcatctgc cttgatgagg ttcaggtctt cgaccgagat caggttctca
     4741 cgtttcaggt taccgattgc cttctccaca ttcttccacg aggtgttctg ggtcagtatg
     4801 gcccctagta tgcactcaag agcggtctcc cccggccacc atccctgcgg gccgaatttt
     4861 ttatgcagct cttcgtagaa tttttttatc cgtgcgctct tggtcattgt ttatatccgg
     4921 gacaggatgt gccgcagtga ataatataca ccgggcgcat caaaaggggg actacttcgt
     4981 tatggcgttg tttggagcct tctgggcctg ctcgtaatag ggcagggcct ggggcagcca
     5041 ttgtttgaga ttatttatcc tggtcccata gcccggatgc gtggacagga attcgggcgg
     5101 ctgcggcttg cccttgacgg ccgcatccat tctctcccag aacgctatcg cctccctcgg
     5161 gtcgtatccc gctttcgcca tatatacgag ccctatgtga tcggcttcgg cttcctgtgt
     5221 cctgctgaac ggaagtatgc cgccgaccgt cacgccgatg ccgtacgcct gcattatcgc
     5281 ttcggatacc cacgggtcgc tcccgccgag agccgcgctc actcccactg cccctatctg
     5341 cgcgagcacg tccgtcgata ctctctcacc gccgtgccgc gccgcgacgt gcgcgacttc
     5401 atgggccata acagttgcga gtcccgcgtc gttctgggct acagacagga tgcccgtgta
     5461 caccccgacc ttgccgcccg ggagcgcgaa ggcattgatc gtctcgtcgt cgttaaaaac
     5521 cttgaagtcc cagttgtatt ggggcgtatc tgcaaccccc actatgcgtg tgcccactct
     5581 ggtcacggct tcgttgtact gcgcgtttgt cgataccttc tcctccttca ggacttcctg
     5641 aaaagccgcg acgcccatct ggttctcttc cgactgggag aggagtatga attgagatct
     5701 cccagtcaca ggcgctttgt aacaggcgac ggcgatagct cccgccaaca ccataacgag
     5761 tactaataac aggggcttga ctagccgccg cccagcagtt ttccctggag aagatttcat
     5821 aattgattaa gttttatcga tttaacggcg atttgcaata ataatttatc cggcttcgcg
     5881 attggaataa aaaggcggat ccaagctttt atcccccccg cccgcgtgag catacacaac
     5941 gtgggataaa agcttggatc cgtaaatccg gtatcaggcg acctttttaa ggtccgcgtt
     6001 tttaattctt ctaatgcctt cgtctatgtc ctcatcggcg catgcgtaag taaccctgac
     6061 gtatccttcg ccctggcttc cgaacgcagt accggcgacg accgcaacgc ccgccgtctc
     6121 gacgagcctg tccgtgaatt cctgtgaggt cagaccggtt cccgtgatat ccgggaacgc
     6181 gtagaacgct ccgtccggcg tgtcgcactg gattccgggt atggagttca gcccggcgac
     6241 catcctgtcc ctctttttct tgaggagctc ccttttctca atcgtccagt cctgcgggcc
     6301 cgccattgcg gcgtaggcgg ccttctgtat gaaagcggct acgttcgtga tcgagtcctg
     6361 taggaagatc gagagcttgt ctatgaccgg cacaggcgcg accgcccagc ctatcctcca
     6421 cccggtcatg gcatacgttt tggagaatgt gtcgatgact atcgtccttt cctccattcc
     6481 ggggacggcc gaaatcgtct tatgcggctt gtcgtaggtc acgtgcgaga aaatttcgtc
     6541 ggatatgacc atgaggttcg ggtacttgaa gcaaagctcg gcgatctctt ccgggttctc
     6601 gacaaggtgt ccgttcggtc tctgcggggt atttatgatg agaagcttcg tttggggagt
     6661 gatcattttt tcgagctttt ccaagtcgaa ctgccagtcg ggtttggtaa gaggggtgtg
     6721 gttgatcttc gctctcacgt atttggccca tacctcgtcg ggcggataac cggggttcgg
     6781 gaatataacc tcgtccccct cttcgaggaa cgtgaggaga cacatggtga gggcgtgctt
     6841 cgcgcccgcg gttactatga cctcttcagg ctttgtcttt ctcccctccc tcgtcatctc
     6901 ggcggctatg gccgctctca gctcgggcag tcccggaccc gggtcgtacc tgacgtaccc
     6961 gtccttgagc gcctgaaccg tagcttcgag aacgtgatcc ggcgtataga aatccggccc
     7021 ctgatagtcc cctttctcga aatggattat cttctgcccg gtcttttttt ccagctcccg
     7081 ggcgaccctc atgctggccc attgcttcgg tggaacgaaa tggctgatct tggaactgaa
     7141 tggatatgag acggccattt gtaatacctc cttgatgaat gatttgtgaa caactgctcc
     7201 tacatcctcg cggcttcgga catttcataa ccgggggcca ggattccctc ggaaatcata
     7261 gaaaatacct catccgcgat actttccgga ctcttttcgc ttttatagta gaacattacc
     7321 gaccttacta gaccgaatat aatgagggcc gtaagctccg tattcgacga tctgaaagac
     7381 ccttcgttga tccctttctc gagtatcttc ctgtaaagct taattctcct cagcctccag
     7441 tgctcggtga attcgtagtg ctccctgatg aggtgagttt cgtccctgct gagtatgcgc
     7501 agcacgtcca ggttctggag gtagaatttg aaaatgaggg cgagcatttt tttgagcttc
     7561 accggggccg ggtcgctctt ggccgtttcc ttctcgagaa tcgac
//
LOCUS       DASSJH010000286         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_178832, whole genome shotgun sequence.
ACCESSION   DASSJH010000286 DASSJH010000000
VERSION     DASSJH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178832"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFJ67_06185"
     CDS             <1..326
                     /locus_tag="VFJ67_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290011.1"
                     /translation="ESYGCGFILTTYPYGHQVSPDEWMPGRYRYLESADFTISDRTID
                     ELARFSAANGIPFLNTFPDFRGYRGREKLYFSHDPHWTPAGQIIMTDALYNFLKPYLA
                     KKLRR"
     gene            364..1767
                     /locus_tag="VFJ67_06190"
     CDS             364..1767
                     /locus_tag="VFJ67_06190"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7290012.1"
                     /translation="MRKTVKLLYADIIVSLMLAGGLVVRSILLGKPLGAITLPVLIFL
                     VVNALFCALSAWGIRRGRGKTAVGVWIFCLSLIVFYLTLDLLGGYLFIPGREITTTRL
                     PDEYAHHKYAPSKTQRAYDPADFDVSVRINNMGFRGRDVGEKKDPGAYRIVMLGDSFT
                     FGAGVGDDQTFSSVLEKILNASSPGKYEVVNCGVESFSPVLEYIQLKRNIDRLKPDMV
                     IMNFDMSDLMNELVYRKEASLGGDGEPLAVSGMEDYRRSREGTMRRLRSWVYENLFIT
                     TALIETIGKRFRGDTGIEDVNVETGIEREWMMMLIHTLKNFPQPENSAEMYGMVEDSI
                     LRTKKLCEAEGCVFVLTTYPWGHQVSDDEWKPGRYEWLPRETNLSNISDRTVEELDRF
                     AARNEIPFLNTFPAFRNYRGSGRLYYTHDIHWTPAGHRLMAETLASFIEARTGAAIDS
                     ALKCRMIGVFIDQDNCK"
     gene            complement(1742..2404)
                     /locus_tag="VFJ67_06195"
     CDS             complement(1742..2404)
                     /locus_tag="VFJ67_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7290013.1"
                     /translation="MTDASVFVITPHKIIYNGIINTLNLETINLNFIWVEDYLCLLNF
                     LHKSDISIFIIDCTGKHQDYLEVITVIKGKIPSANILSIIEDQNADTELKLLSIGTRG
                     IITENIGINLFNKAVLEVLKGNMWIRRKILELFVEKTLYFEELCVQLFENRFSRLSSK
                     EIEILRLASVGLTNTEIAISLFLTEKTVKNYLTRIYKKLHIHGRSEINTKVKPIYNYL
                     DQ"
     regulatory      complement(2501..2586)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2882..>3653)
                     /locus_tag="VFJ67_06200"
     CDS             complement(2882..>3653)
                     /locus_tag="VFJ67_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290014.1"
                     /translation="NNDIKTRIRGWVMRYIIRILIVPLVFSPLIFLASCCDEAPAELN
                     VYVGHLSERECKELQGSVNDNADDIVFTCKGEEVTLCWGGTVSSIEPGIGSVSSPGIS
                     YQNVTSDTEFVAKPPSGSCASSDSITVNVVTEDTPSTWEGKFDSKCSKIEFEINKEFI
                     SEKIKAKDITALWEPILNIEGGGTFTCSTPPFLDGFHVEEVFGFSISQPHITQAFSRK
                     LKAVGNWQFVWKANCDGYVIKCDPFVSFPFDLTLTCEE"
BASE COUNT          986 a          846 c          871 g          950 t
ORIGIN      
        1 gcgagagtta cggatgcggg ttcatactga cgacctaccc gtacgggcac caggtgagcc
       61 cggacgagtg gatgcccggc aggtacaggt atctggagag cgcggacttc acaatatccg
      121 acaggacgat cgatgagctc gcgagattca gcgcggcgaa cggcatcccg ttcttaaaca
      181 cctttcccga cttcaggggg tatcgcggca gggagaaact ctatttcagt cacgaccccc
      241 actggactcc cgcgggtcag ataataatga cggatgcatt gtacaatttt ttgaagccct
      301 acctcgcgaa aaaactacgg cgttaaggac cgataaacga agccgggcct gccgaccgag
      361 accatgagga agacggtaaa acttctttac gcggatatta tagtgtcgct gatgctggcg
      421 ggcggccttg tcgtccgctc catcttgctc ggcaagccct taggcgcgat cacgctcccg
      481 gtactcattt tcctcgtagt caacgcgctg ttctgcgctc tctccgcatg gggaataaga
      541 aggggacgcg gaaagaccgc cgtcggggta tggatcttct gcctctcact catcgtcttt
      601 tatctcacac tcgacctcct gggcggctat ctcttcatac ccggaaggga aatcacgacg
      661 acgaggctgc ccgacgaata cgcgcaccat aaatacgccc cttccaaaac gcagagggct
      721 tacgacccgg ccgatttcga cgtgagcgtg aggatcaaca acatgggttt cagggggagg
      781 gacgtcggcg aaaagaagga cccgggcgct taccggatag tcatgctcgg ggactcgttt
      841 acgttcggag ccggggtcgg ggacgaccaa accttttcga gcgtcctcga gaaaatcctg
      901 aacgcgtcgt cgccggggaa atacgaggtc gtcaactgcg gcgtcgagag cttctcgccc
      961 gtgctcgagt acatacagct caaaaggaat atcgatagac tcaagcccga catggtgata
     1021 atgaacttcg acatgtcaga cctcatgaac gagctcgttt acaggaaaga ggcgtcgctc
     1081 ggcggtgacg gggagcccct ggccgtaagc gggatggagg attacaggcg gtcgagagag
     1141 gggactatga ggaggctccg gagctgggtt tacgaaaacc tcttcataac gacggcgctg
     1201 atcgagacga tcggcaagag gttccggggg gatacgggca ttgaagacgt gaacgtagag
     1261 acggggatcg agagggagtg gatgatgatg ctcatacaca cgctaaaaaa cttcccccag
     1321 ccggagaact ccgccgagat gtacggtatg gtcgaggaca gcatcctcag gacgaagaaa
     1381 ctatgcgagg cggagggatg cgtgttcgtg cttacgacat acccgtgggg tcatcaggtg
     1441 agcgacgacg aatggaaacc ggggagatac gagtggctac cgagagaaac gaatctttcc
     1501 aacatctcag acaggacggt cgaggagtta gacaggttcg ccgcgcggaa cgagataccg
     1561 ttcctcaata ctttccccgc attcaggaat taccggggga gcggccgcct ctattatacg
     1621 cacgacattc actggacgcc cgccggtcat cggctcatgg ccgagacact cgcttctttt
     1681 atcgaggcgc ggacgggagc ggcgattgat tccgcgctga aatgccgtat gataggagta
     1741 tttattgatc aagataattg taaataggtt tgaccttagt atttatctct gatctgccat
     1801 gaatatgaag ttttttatat atacgagtca aataattctt aacggttttt tcagtcagga
     1861 aaagactgat agctatttcc gtatttgtta aaccgacgga tgccaacctt aatatttcaa
     1921 tttcttttga agataaccgg ctaaaacgat tttcaaataa ttgaacacat aattcctcaa
     1981 aatacagcgt tttttcaaca aacagttcaa ggatttttct cctgatccac atatttccct
     2041 taagaacttc caacacagcc ttgttgaaga gattaatccc tatattttcg gttattattc
     2101 ccctcgtacc tatcgatagc agtttaagct ccgtatcggc attctgatct tcgataattg
     2161 acaggatatt tgcactcggg atttttcctt taatcacagt aataacttca aggtaatcct
     2221 ggtgttttcc ggtacaatct atgataaata tcgatatatc cgacttatga agaaaattta
     2281 aaagacacaa ataatcttcc acccagatga aattaagatt aattgtttca agattgagag
     2341 tattaattat cccattgtag ataattttat gcggcgtgat aacgaatacg gatgcatcgg
     2401 tcattaatta ttcacccggg cagaccggtt gcggtaacga aatacgaatg ggggaaattc
     2461 agcgtgcgcg tgggcagtag gtttacttgg tcaggttcac tataacaatc cagccgccgt
     2521 aaacgggaag ttcttaggcc tgtgattttg cgtcccattc tttcaaatgg tttgcccttc
     2581 tagttattta tcattctaca atagcccgtt atgttgtcaa cattgtacag gtcaatataa
     2641 ctatactctt atatgattaa tcctattcgt tattaatcat atgtgatata gagtgagtcg
     2701 aatacgggaa aaataatcca gccataacca ggttgacccg gccctataac tggtacataa
     2761 gttagatttt ctgtaaagaa tcctattaca ggatgtcaga gaaatggtca ggaaacacta
     2821 caccgcagtg cagataatta tgtattttag gaagaacttc aatttcgctg catgtttctc
     2881 tctattcttc acaagtaagt gtcagatcaa aggggaaact cacaaatgga tcacacttga
     2941 ttacatatcc gtcgcaattg gctttccaaa caaattgcca gttccccact gcttttaatt
     3001 ttcttgagaa agcttgggtt atgtgtggtt gtgaaattga aaatccaaaa acttcctcca
     3061 catgaaatcc gtctaaaaat ggcggggtag aacatgtaaa agtgcctcct ccttctatgt
     3121 tcaagattgg ttcccataaa gccgttatat ctttggcttt gattttttca cttataaatt
     3181 ccttgtttat ttcgaactct attttactgc atttagaatc gaacttgcct tcccaggtgc
     3241 ttggcgtatc ttcagtaacg acattaaccg taatactatc tgaagaggcg caactgccgc
     3301 tcggcggctt ggctacaaat tcagtatcag aggtcacatt ctggtaggag attcccgggg
     3361 agcttaccga gcctatgccg ggttcaatcg atgaaactgt tccaccccag caaagggtta
     3421 cttcttctcc tttacatgtg aatacaatgt cgtcggcatt gtcattaacg gaaccttgca
     3481 attccttgca ttcgcgctct gaaagatgtc ctacatatac attcaattca gcaggagctt
     3541 catcacagca tgaggctaga aatattaaag gagaaaagac caaaggaact attaatattc
     3601 gtataatata tctcattacc caccccctta tacgggtctt aatatcatta ttg
//
LOCUS       DASSJH010000287         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1582155, whole genome shotgun sequence.
ACCESSION   DASSJH010000287 DASSJH010000000
VERSION     DASSJH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582155"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFJ67_06205"
     CDS             complement(<1..295)
                     /locus_tag="VFJ67_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7290015.1"
                     /translation="MPGRENPKRQDLIDFLLESSSYPHNPPGVTHLQTHISDVFIAPP
                     FVYKVKKPVDLGFLDFSTLEKRKHFCHIEVELNRRLCPGVYLGVEDITAADGKL"
     gene            complement(317..1225)
                     /locus_tag="VFJ67_06210"
     CDS             complement(317..1225)
                     /locus_tag="VFJ67_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7290016.1"
                     /translation="MEIKRILWATDGSGEAELALEYAKYLARVSGAEIIGVHVIPLPV
                     QLLFESLREEDEEFKEWQSKVERRVSDRFVEVRNELQHAGIKFDGVILKGIPSDKIRE
                     FSRLRKADIIVMGKQGHGLLESVMVGSETIKVLKTSHIPVLAVKRRGAGTDIQIKNIV
                     VPIDLADENETALNCALDIAGMTGASVTAVYALRLDMYAQDMPAGALDIVIKQSVKEL
                     RERTDGMKAKYESTRGTQVKGGIKTEVIHGLSPAISIANYASRNDADLIVINTHARTG
                     LKRLVLGSVTERMIPESPCAVLSLRP"
     gene            complement(1334..1783)
                     /locus_tag="VFJ67_06215"
     CDS             complement(1334..1783)
                     /locus_tag="VFJ67_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7290017.1"
                     /translation="MSAKRKSQAELLGEVINKNVVTVSPTAAISEAAYLMMNEDVGAL
                     VVVDTDMRPVGMLTDRDLVVSAMAEGVSPDEATVEQLMTKDLITVDEDTDIFEMLSIL
                     SENSIRRLPVVRGGKLKGIVSVDDLIVVVATELVNLAMALSSKSKVL"
     gene            2081..2323
                     /locus_tag="VFJ67_06220"
     CDS             2081..2323
                     /locus_tag="VFJ67_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290018.1"
                     /translation="MSDYGTIYLIETNYNADRDAREVTFGYIEKEEEVKGRIMSLRVI
                     VNVPGHRDDVKGAVEAGLAKARALASRAAGAAYEPD"
BASE COUNT          478 a          660 c          627 g          574 t
ORIGIN      
        1 agagcttccc gtccgcggcg gttatgtcct cgacgccgag gtaaacgccc gggcagaggc
       61 gcctgttgag ctcgacttct atatgacaga agtgcttccg cttttcgagc gtgctgaagt
      121 cgaggaagcc cagatcaacc ggttttttta ccttatacac gaatggcggg gctatgaata
      181 cgtccgatat gtgcgtctgg aggtgcgtga cgcccggggg attatgagga taggaggaag
      241 attccaggag aaaatcaatg aggtcctgtc ttttcgggtt ctctctgccg ggcatcttat
      301 ccaaaggtcg aatttattac ggccttaacg acaatacggc gcagggggat tcagggatca
      361 tcctctcggt gacgctcccc agaacgagcc tcttcaatcc cgtcctcgcg tgggtgttta
      421 taactatgag gtcggcgtcg ttcctggagg cgtaattcgc aatcgatata gcagggctga
      481 gcccgtgtat cacctccgtc tttatcccgc ccttgacctg agtgccgcgt gtgctctcgt
      541 atttcgcctt catgccgtcc gtcctttccc tgagctcctt gaccgactgc tttatcacta
      601 tgtcgagagc gccagccggc atgtcctgcg cgtacatatc gagcctgagc gcgtaaactg
      661 cagtcacgct cgcgcctgtc atgcccgcga tgtcgagggc gcagttaagg gctgtctcgt
      721 tttcgtcggc caggtcgatg ggaacgacta tatttttgat ttgtatatcc gtcccggctc
      781 cgcgccgctt gacggcaagc acggggatgt gtgaggtctt gagcaccttt atagtctcgc
      841 ttccgaccat cacgctctcg agcagcccgt gtccctgctt acccataacg atgatgtcgg
      901 ctttcctcag gcgggagaac tcccttatct tgtcgctcgg tatccctttc aggatgacgc
      961 cgtcgaactt tatgcccgcg tgctggagct cgttcctgac ctcgacgaac ctgtcgctca
     1021 ccctcctttc caccttagac tgccattcct tgaactcttc gtcttcttcc ctcagggact
     1081 cgaagaggag ctgcacgggc agcggtatga cgtggacgcc tatgatctcg gcgccggaga
     1141 cgcgggccag gtatttcgcg tactcgagag cgagctccgc ctcgccagac ccgtctgttg
     1201 cccagaggat tctcttgatt tccatatttg tctctcttta tagaatttgt tccctattcc
     1261 gcttccgttc ggacggtccg ccggggacac ataattctac cacattatcg ccgcttatgt
     1321 cccccgcccg ggctcagagg accttcgatt tgctgctgag ggccatggca agattaacga
     1381 gctcggtcgc gacgacaacg atgaggtcgt ccacagacac gatccccttg agcttaccac
     1441 cgcggaccac cgggagtctc ctgatagaat tctcggaaag tatgctcagc atctcgaaga
     1501 tatccgtatc ctcgtccacg gttatgaggt ccttggtcat taactgctcg acggttgcct
     1561 cgtcagggct aacgccctcc gccatcgccg acacgactag gtccctgtcc gtaagcatgc
     1621 cgacgggtct catatcggtg tccactacga cgagcgcccc tacgtcctca ttcatcatga
     1681 ggtacgcggc ctctgaaatc gccgccgtcg gagagacggt gacgacgttc ttgttaatga
     1741 cctcgcccag gagctcggcc tgtgattttc tttttgcgct catttattct tcctccccgg
     1801 aaggcaatcc gtattacaaa attttactga taaatagtgc aacattggtg ccatcggaac
     1861 aagcagggtt ttacgagcga aaacccgcgt aatcccgcct gattctcgac tgctccctgt
     1921 cgtgtgtcgt attttcagac atacaagggt aacattaaca tcaaaaatat gctttgtgta
     1981 atgaggattg agaaatggca cctttgctgc ttcatttcgg taagatccaa tataaacaaa
     2041 gcgtgtggca gaggcatacg gattaaaccg ggagacgctc atgtccgatt acggaaccat
     2101 ttacctcatc gagacgaact ataacgcgga cagggacgcg agagaagtaa cgttcggata
     2161 tatcgagaag gaggaggaag tgaagggcag gataatgtcg ctcagggtaa tcgtgaacgt
     2221 tcccggacac agggacgacg tgaagggcgc tgtcgaggcc ggtctcgcca aagcgagggc
     2281 gctcgcctca agggcggcag gggctgctta cgagcccgac tgaggcgccg agtcttatc
//
LOCUS       DASSJH010000288         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_503743, whole genome shotgun sequence.
ACCESSION   DASSJH010000288 DASSJH010000000
VERSION     DASSJH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503743"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFJ67_06225"
     CDS             <1..435
                     /locus_tag="VFJ67_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7290019.1"
                     /translation="EGLRKILDALLELDLKYINSRTDAGMALSEKIPDAVVRQFLLQN
                     LKQNTAGEFSWRCNLPELYRFVANETFELPAEAVYEGPTLVIAGGRSEYKVWEHEKSL
                     RAHFPAMILEIFEDAGHWVHMDEPDRLVHRLREWVSVRASRA"
     gene            610..2247
                     /locus_tag="VFJ67_06230"
     CDS             610..2247
                     /locus_tag="VFJ67_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7290020.1"
                     /translation="MLKQMTVGQYLIKRLYDLGVRHVFGVPGDYALSFFKEIEESKLK
                     LINTSDEQGAGFAADAYARVKGLGAVCITYCVGGLKVANTTAQAFAEKSPVVVISGAP
                     GIKERLRNPLLHHKVREFDTQLKVFRELTIDSVLIDDAETAAFEIDRVLNSALRYKRP
                     VYIELPRDIVALPIKPYKAQRRETEGSEAETLNEALAEATAIINNSKKPVIIAGIEIH
                     RFGLQDELMELLDKTNIPFVTTILGKSVISETHPLCIGVYEGAMGREAVREYVESSDC
                     LILLGAFMTDVNLGIYTAKIDRTNTIYATSEGVSIKYHTYEDISINDFIDGLLKGSIR
                     RRKSAKIPNPPLPRHPRPVKGRRVSVSFMFRTLNSHLNEKTVVIADPGDAMLGALDMV
                     IQRETEFLCPAYYCSLGFAVPASLGVQAARPDLRPLVLVGDGAFQMTGMELSTIVRFK
                     QNPIIIVFNNSGYGTERPMIDGRFNDVLNWNYSRLPEVLGSGIGFDIETEDELEEALT
                     KALGDKNNFYILDVHLDPEDRSEAHKRLTNALAKIVR"
     gene            complement(2366..>3328)
                     /locus_tag="VFJ67_06235"
     CDS             complement(2366..>3328)
                     /locus_tag="VFJ67_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290021.1"
                     /translation="SALEVQSRNENTLFDNPGVVAVGVGLTEDGSGPAVHVYVNTDAP
                     GASASAISSRINGVPVRVFETDEIMALDAPPGSNHKQTFAPPVPMGVSTGSVSISGGF
                     VTIGTLGYRVFRLGQPNNVGYITNNHVAGSSGANLCPAMINPANLPPFRLAQCQPSLA
                     DGGGCVSSVGNLVQAIPILMGALYQNTVDAAFVKSNRNLVNKTILDIGNPSPQLQAPD
                     VNLTVWKSGRTTGFTQGTIQSINVTEIVSYGACGVAKFVGQIAIVPGTFSGAGDSGSP
                     VLGDTDSSGRRRPVGLLFAGSSAVTFANRISDVLGALHSAIDTR"
BASE COUNT          841 a          868 c          911 g          708 t
ORIGIN      
        1 gaggggctca ggaaaatact cgacgcgctt ttggagctcg acctcaaata cataaattcc
       61 cgaaccgacg ccggcatggc tctctcggag aagatccccg acgccgtggt gcgccagttt
      121 ttacttcaga atctcaaaca gaataccgcg ggggaattct cgtggcgctg caaccttccc
      181 gagctctaca ggttcgttgc gaatgaaacg ttcgagctcc ctgcggaggc ggtctacgaa
      241 gggccgaccc tggtgattgc agggggtaga tctgaatata aggtctggga gcacgaaaag
      301 tccctaaggg ctcatttccc cgccatgatt ctcgagatat tcgaggacgc cggccattgg
      361 gtgcacatgg acgagccgga ccgtcttgta catcggttgc gtgaatgggt gagcgtacgc
      421 gcctccagag cctgaggtat taccggcggg acgggttatt caattttcct ccgcatactg
      481 tacattccgg aaccaagtaa aagttttcat acgtgtaatg gattaaagtg agcggcatcc
      541 cctttttgac atggttcgag atgggattaa atttagggtg atgaaatttc cggtgacgga
      601 ggactcctca tgttgaagca aatgaccgtc ggccaatacc ttataaaaag gctttacgac
      661 ctgggcgtcc ggcacgtctt cggcgtcccg ggcgattacg ctctcagttt cttcaaggaa
      721 atcgaggaga gcaagctgaa gctgataaac acaagcgacg agcagggcgc ggggttcgcg
      781 gctgacgcct acgccagggt aaagggactc ggcgcggtct gcatcacgta ctgtgtcgga
      841 ggactcaagg tcgcaaatac aaccgctcag gcatttgccg agaaatcgcc cgtcgtggtc
      901 ataagcggag cccccggaat caaggagcgc ctcaggaacc cgctgctcca tcataaggta
      961 agagagttcg atacgcagct caaggtgttc cgggagctga cgatagactc cgtgctcata
     1021 gacgatgctg agactgccgc attcgagata gatcgcgtgc tcaattcagc attgaggtac
     1081 aaaagaccgg tatatataga gctgccgcgt gacatcgtag cgctccccat aaaaccctac
     1141 aaggcgcaac gccgggaaac agagggcagc gaggctgaaa cacttaatga agcgctcgcg
     1201 gaagcgaccg caattataaa caattcaaaa aagcccgtca taatagcggg gatcgagata
     1261 caccgtttcg gccttcagga cgagctcatg gagctcctcg ataaaacgaa tatacctttc
     1321 gtgacgacga tcttgggaaa atccgtcatc agcgagactc atccgctctg catcggcgtg
     1381 tacgaggggg cgatggggag ggaagccgtc cgggaatacg tggagtcgag cgactgcctg
     1441 attctcctgg gcgcttttat gacggacgtg aatctcggaa tctatacggc gaagatcgac
     1501 cggacgaaca caatatacgc cacgagcgaa ggcgtgtcca tcaaatatca tacgtatgaa
     1561 gacataagca taaacgattt tatagacggg cttttaaaag gaagcatccg ccgcaggaag
     1621 agcgcgaaga ttccgaaccc gcctctgccg agacacccgc gtccggtcaa gggcaggagg
     1681 gtcagtgtga gtttcatgtt caggaccctt aactcgcatc tgaacgaaaa aaccgtcgtg
     1741 atcgctgacc ccggggacgc gatgctcggc gcactcgata tggtcattca gagggaaacg
     1801 gaatttttgt gtccggcata ttactgctcg ctcgggttcg cggttccggc aagcttgggc
     1861 gtgcaggcag cgagacccga tctccgccct ctcgtgctcg tcggggacgg cgctttccag
     1921 atgaccggga tggagctgtc aacgattgtc agattcaagc agaacccgat cataatagtg
     1981 ttcaataaca gcggttacgg tacagagcgc ccgatgatcg acggcagatt caacgatgtt
     2041 ctgaactgga attatagccg cctgcccgaa gtccttggga gcgggatcgg tttcgatatc
     2101 gaaacggagg atgagctgga agaggccctg accaaggcgc tcggcgacaa gaacaacttt
     2161 tatatcctcg acgttcacct tgatccggaa gacaggtctg aggcacacaa aaggcttacg
     2221 aatgcgctgg caaaaatagt gagatagcag gctgaataat attgatccgg ccgaatgtaa
     2281 aagcgtaggc gaccgatacg taattgcggc agtacatgac aacagtggca tccttcatga
     2341 ccagtgaatc gtatctacgc agtgactatc tagtatctat tgcagaatgc agtgccccta
     2401 aaacatcgct gatacgattt gcaaatgtaa cagccgatga accggcaaat aggagcccga
     2461 ccggtcttct gcggcctgat gaatctgtgt cgccaaggac aggcgacccg gagtcccccg
     2521 ctcccgagaa ggtacccgga acaatagcta tctgacctac aaacttagca accccgcatg
     2581 caccgtaaga cactatttcc gtgacattaa tcgattgtat agtaccctgc gtaaatcccg
     2641 tagtcctgcc gcttttccag acagtcaggt taacgtcagg agcctgcagc tgcggactcg
     2701 gattcccgat gtccagtatg gttttattca ccaggttgcg attgctcttt acgaaagccg
     2761 catcaaccgt attttgataa agtgccccca ttagaatagg aattgcctgg accaggtttc
     2821 ctacggagga tacgcagccg ccgccatcgg ccagagaggg ctggcactga gccaggcgga
     2881 atggaggcaa atttgcgggg ttaatcatag ccgggcagag atttgcaccc gagcttccgg
     2941 caacatggtt gttcgtgatg taacccacat tatttggctg ccccaaccga aagactctat
     3001 atcccagagt cccgatcgta acaaatcccc cggatatgct gacactaccg gtggatacgc
     3061 ccatgggaac ggggggagcg aatgtctgct tatggttact tcccggcgga gcgtccaatg
     3121 ccataatctc atctgtttcg aataccctca cgggcacacc gtttatccgg ctcgaaatgg
     3181 cagaagccga ggctccgggt gcgtccgtat ttacgtatac gtgtacagca ggcccgctcc
     3241 catcctcagt taaacctacc cctactgcaa cgacgccggg gttatcaaaa agtgtatttt
     3301 catttctact ttgaacctcc agagcgga
//
LOCUS       DASSJH010000289         5133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_70796, whole genome shotgun sequence.
ACCESSION   DASSJH010000289 DASSJH010000000
VERSION     DASSJH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70796"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..484)
                     /locus_tag="VFJ67_06240"
     CDS             complement(65..484)
                     /locus_tag="VFJ67_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET7290022.1"
                     /translation="MAEMISDKDEVMFPELFRTDEQGRVRLVAGYSKESNNWTFPKYL
                     ADPVSFSDEVEERLLSPTGELHSFTIVRRSMPEFPVPYALALVDFPEGVRVMAQVETS
                     DPEGLKIGQRMGVTVGTVKKSKDGKDVKSYKFVPVKN"
     gene            607..1407
                     /gene="thiD"
                     /locus_tag="VFJ67_06245"
     CDS             607..1407
                     /gene="thiD"
                     /locus_tag="VFJ67_06245"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET7290023.1"
                     /translation="MGRKVPRALTIAGSDSGGGAGIQADLKTFTAFRVYGMSVITSVT
                     AQNTEAVLGIFDLPGEFVGLQLDAVLGDIGADAVKTGMLSNKEIVSAVSGKIRQYGVK
                     NLVVDPVMISKGGAALLDTEAREAIVEELFGLALIVTPNIPEAEEITGLEIKNLADMK
                     RAAEDIKKTGPGYVLLKGGHLETLADAIDVFFDGQSHYELKAPRIGTKNTHGTGCTYS
                     AAVCAALSQGFSPLDAVKEAKDYVTYAIMRSFELGKGHGPLNHFWKFE"
     gene            1424..2278
                     /gene="folD"
                     /locus_tag="VFJ67_06250"
     CDS             1424..2278
                     /gene="folD"
                     /locus_tag="VFJ67_06250"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018393884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET7290024.1"
                     /translation="MAEIIDGKIVSQHIRAEVAEGVERLRREAGITAGLAAVLVGDNP
                     ASEIYVRNKRKACSEAGIYSEEHRLPGETKEEELLSLVEKLNADSNIHGILVQLPLPS
                     QISETKILRAVSPLKDVDGFHPYNVGLLVEGKPRFISCTPHGIIKMLDFYKLGIEGKE
                     AVVVGRSNIVGKPVSMLLLHRHATVTICHSRTKPLDEVTRRADILVAAIGRADFITAD
                     MVKEGAVVIDVGINRKEDGKLTGDVDFEGVSKKASYITPVPGGVGPMTIAMLLFNTLE
                     SARNFASS"
     gene            complement(2466..2966)
                     /gene="folK"
                     /locus_tag="VFJ67_06255"
     CDS             complement(2466..2966)
                     /gene="folK"
                     /locus_tag="VFJ67_06255"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017550036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7290025.1"
                     /translation="MPRAFISLGSNLGDRVLNCKKALGEISGFAEIRGVSSVYETEPV
                     GYDDQPYFINCAAEIETALPPLELLDRLREVEKGLGRTRSEAWGPRTIDIDIIFYDDL
                     VIDTEELTIPHVSAHARRFVLEPLCEINPMFVHPGFGVRVYLLLNNLDDDKKVVKAGE
                     PSTLYP"
     gene            3043..3318
                     /locus_tag="VFJ67_06260"
     CDS             3043..3318
                     /locus_tag="VFJ67_06260"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET7290026.1"
                     /translation="MPLLTDAEIEDGLRGLNGWERKGSEIVRVFKNKSFVDSVGFVNK
                     VSILAEKADHHPDILIQYKNVTLTLSTHSKGGLTEKDFNLAREIDAL"
     gene            complement(3331..3603)
                     /locus_tag="VFJ67_06265"
     CDS             complement(3331..3603)
                     /locus_tag="VFJ67_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290027.1"
                     /translation="MNSRPKIFFYQGKPPAYLIPLVILLVIVVFAVLAVFGLFLGIAV
                     GIALIIFGVMRLFSPNGKSKYRTIEEDGRTTIVLDKKDYEVIEKGK"
     gene            complement(3678..4619)
                     /locus_tag="VFJ67_06270"
     CDS             complement(3678..4619)
                     /locus_tag="VFJ67_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7290028.1"
                     /translation="MEPSEFTARIKNLEVNISGVIKGKPEIVKAAIVALLSGSHMLIE
                     DVPGVGKTTLAHALARSTDLSFKRIQFTSDLLPSDILGVSVLDQSSGEFKFKPGPIFA
                     NVVLADEINRATPKTQSALLEAMNEAKATIDGAVCDMPLPFMVVATQNPFEYKGTFPL
                     PENQLDRFALRISIGYPDRESEKEILSAPGAANPWDGLTPVISREEVILLQKLSGSVR
                     TDDSILDYILSIVAETRNPKLFELGVSPRGAKALKSVSQSLALTEGRTYCIPEDVKKM
                     AVPVLAHRVVVRSERLTGRGAEEEAIKEVLDRVKVPI"
     gene            complement(4631..>5133)
                     /locus_tag="VFJ67_06275"
     CDS             complement(4631..>5133)
                     /locus_tag="VFJ67_06275"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET7290029.1"
                     /translation="TVNGREVAIEHIRKKVHEFYETKESEITPETMRQIERYIMLQTL
                     DYLWKDHLLNMDHLREGIGLRGYAQRDPLHEYKREGFDMFSSLINRLSYDVCEKLFRV
                     QPVSETDMERLERRRRAEQQRMTLSRGEEEESKKPVKRNDKKVGRNDPCPCGSGKKYK
                     RCHGAT"
BASE COUNT         1114 a         1382 c         1387 g         1250 t
ORIGIN      
        1 tgccccgtta agctgccgtt aaaactgaaa tgacttgctg cttcggggag ataagcaggc
       61 tctgtcaatt cttgacggga acgaatttat aagatttaac gtccttcccg tctttggatt
      121 ttttgactgt ccctactgta acacccatcc tttgaccaat cttcaatccc tcgggatcgc
      181 tggtttccac ctgggccata accctgacgc cttccggaaa gtccacgagc gcgagcgcat
      241 aagggaccgg aaactcgggc atgctcctcc ttacgatcgt aaaggagtgg agctcgcccg
      301 tcgggctaag gagcctttcc tctacctcgt ccgagaagct gaccgggtcc gccagatact
      361 tggggaacgt ccagttattc gactcttttg aatatccggc tacgagcctg accctgccct
      421 gctcgtccgt cctgaacagc tccggaaaca taacctcatc cttgtcagat atcatctctg
      481 ccataactcc gatatctcct tttgtaattt ttgctgagga atatattaca tttaaatttt
      541 atttagtcta gggcggggaa tccgctcgtt tgtaaaggtt ataaaacctt tgtagaataa
      601 aacggcgtgg gtcgaaaggt tccgagagca ctgacgatag ccggttccga ttcaggggga
      661 ggggccggga tacaggcgga cctgaaaacc tttaccgcct tccgggttta cggtatgtcc
      721 gtgattacgt ctgttacggc tcaaaatacg gaagcggtgc tcggaatatt tgaccttcct
      781 ggggagttcg tcgggctcca gttggacgcg gtcctgggcg acataggcgc cgatgccgta
      841 aaaaccggca tgctgtcgaa taaagagatc gtatcggccg tatccgggaa aatccggcaa
      901 tacggggtta aaaatctggt agtagatcct gtcatgattt ccaaaggagg ggcggcgctc
      961 ctcgatacgg aggccaggga ggctatagtg gaagagcttt tcggcctcgc gctgattgta
     1021 accccgaata tcccggaagc ggaagagata accgggctcg agataaagaa tcttgccgac
     1081 atgaagcggg cggccgaaga cataaagaaa acgggcccgg gatacgtgct cctgaagggc
     1141 ggccatctcg agacactcgc agacgctata gacgtcttct tcgacggaca aagccactac
     1201 gagttaaaag cgccccggat cggcacgaag aacacgcacg gcaccgggtg cacgtattcg
     1261 gcagccgttt gcgccgcgct ttcacagggg ttttccccgc tggatgctgt aaaggaagcc
     1321 aaggactacg taacttacgc catcatgcgc tctttcgagc tcggcaaagg gcacggaccg
     1381 ctgaaccatt tctggaaatt cgaatgacca aggagagtct cgcatggctg aaatcatcga
     1441 cggtaagatt gtctcgcagc acatcagggc cgaggtcgcg gagggcgtgg aaagactcag
     1501 gagagaggcg ggtataacgg ccggtctcgc ggccgtgctg gtcggggata acccggcgtc
     1561 cgaaatctac gtcaggaaca agagaaaggc gtgctccgaa gcgggcattt attcagagga
     1621 gcacaggctg cccggggaga cgaaggagga ggagctcctc tcgctcgtcg agaagctcaa
     1681 cgctgacagc aatattcacg gcatactggt ccagcttccc cttccctcgc agataagcga
     1741 aacgaagata ctgagggccg tgtctccctt gaaagacgtg gacgggttcc acccctataa
     1801 cgtcggcctg ctcgtcgaag ggaagccgag gttcatttcg tgcaccccgc acgggataat
     1861 aaaaatgctc gacttttaca agttaggcat agagggtaag gaagcggtcg tagtaggaag
     1921 gagcaatatc gtaggcaagc ccgtctccat gctgctgctt caccgccatg cgacggtcac
     1981 gatatgccac tcgcgcacga agcctcttga tgaagtgacg aggagggccg atatattggt
     2041 agccgcgatc gggcgcgccg atttcatcac tgcggatatg gtcaaggaag gagcggtcgt
     2101 aatcgacgtc ggcatcaacc gcaaagagga cgggaagctc acgggggacg ttgacttcga
     2161 aggcgtcagc aaaaaagcct catacatcac gcccgttccg ggtggggtag ggcctatgac
     2221 gatcgcgatg ctgctcttta acacgctcga gtccgccagg aatttcgcgt cctcctgaaa
     2281 acgccccgcc ttgtggataa aaggccggaa ccacccgatt agttatccac agggctatta
     2341 ttcgggagtg atctgacatt ttaacttgcc gtaaattctc cattattttc tcggtgggtc
     2401 gccggtatcg ctgttggcag gattcttgag atattgtttc aatactgtgg acacgatgtg
     2461 aattttcatg gatataatgt ggatggctct cccgccttaa ccactttctt gtcgtcatcg
     2521 aggttgttaa gcagcaggta aacgcgtacg ccgaatcccg ggtgaacgaa catcgggttt
     2581 atctcgcata agggctcgag cacgaaccgt ctcgcgtggg cgctcacgtg aggtatcgtc
     2641 agctcctccg tatcgattac gaggtcgtcg taaaatatga tatcgatgtc tatagtcctc
     2701 ggcccccatg cttcgcttct ggtccgtccc aatccctttt cgacttcccg caggcggtcg
     2761 agcagctcaa ggggagggag cgctgtctct atttccgccg cgcagtttat gaaataaggc
     2821 tggtcatcgt atccgacggg ctccgtttca taaacggaag acacccctcg aatctcagcg
     2881 aagcccgata tctctccgag ggcctttttg cagttgagga ccctgtcccc gagattagaa
     2941 cccagactaa tgaacgccct cggcatcagt taagtgtaaa taacaaagtt ttgactatcc
     3001 atccgctctc cataaaataa ataaagtggg aggtgaacac taatgccgct gttaacggac
     3061 gccgagatag aggacggtct gagaggactc aacggttggg agagaaaggg gagcgagata
     3121 gtcagggtgt ttaagaacaa gagcttcgtc gattccgtcg gttttgttaa taaggtatcg
     3181 atactggccg agaaggccga ccaccatccc gacatactta tccagtacaa aaacgttaca
     3241 ctgaccttat cgactcacag caagggcgga ttaacggaga aggattttaa tctcgcgcgg
     3301 gagatagacg ccctttaagc ggaggggata ttacttgcct ttctctatga cttcgtagtc
     3361 tttcttgtcg aggactatgg tcgtcctgcc gtcctcttct atagttctgt atttgctctt
     3421 gccgttcggg gagaagagcc tcattactcc gaagataatg agcgcgattc ctactgctat
     3481 cccgaggaaa agtccgaaaa cagccagtac cgcgaatacc acgataacga gcagtatgac
     3541 gagcggtatt agataggcgg gcggtttacc ctgataaaag aagattttcg gtctgctgtt
     3601 cattttctcc tccgcttcga atacctgatt ccattagtac ccgtatatat tctaccatgc
     3661 ccggggtcga aattccgtta aatcggcacc ttcaccctgt cgaggacttc ttttatcgcc
     3721 tcttcctcgg ctccgcgtcc ggtgaggcgt tcgctcctga cgaccaccct gtgcgcgagc
     3781 acggggaccg ccatcttctt cacgtcctcc gggatgcagt acgtcctccc ttccgtgagc
     3841 gcgagcgatt gagagacgct ctttaacgct ttcgcccccc tcgggctcac tcccagctcg
     3901 aacagcttgg ggttcctcgt ttccgccacg atcgagagta tgtagtcgag tatagagtca
     3961 tcggtcctga cgctccccga cagcttctgg agcagtatga cctcttccct cgatataacg
     4021 ggggtcaggc cgtcccaggg gttcgccgcg cccggcgccg aaagtatctc tttttcgctc
     4081 tccctgtccg gatacccgat gcttatcctc agcgcgaacc tgtcgagctg gttctccggg
     4141 agtgggaacg tgcccttgta ctcgaacggg ttctgggtcg cgacgaccat aaacggtaga
     4201 ggcatatcgc agacggctcc gtcgatagtc gccttcgcct cgttcatagc ttcgaggagc
     4261 gcgctctggg tcttgggggt cgccctgttg atctcgtcgg cgaggacaac gtttgcgaat
     4321 atggggccgg gtttgaattt gaattccccg gaggattgat cgagaacgga tacccccagt
     4381 atgtcggacg gcaagaggtc gctcgtgaac tgtatcctct tgaagcttaa gtccgtcgac
     4441 ctcgcgagag cgtgggccag cgtcgttttc cctacgccgg gtacgtcttc gataagcatg
     4501 tgcgacccgg agaggagggc cacaatagca gcttttacta tttcgggttt cccctttatt
     4561 actccgctaa tgtttacttc gaggtttttt atcctggctg tgaactcgga cggttccaaa
     4621 cgagatcctc tcaggttgcg ccgtggcatc tcttatattt ctttccgctg ccgcaggggc
     4681 aggggtcgtt tctccccacc ttcttgtcgt ttctcttcac gggcttcttg ctctcctctt
     4741 cctcccccct gctgagcgtc atccgctgct gctcggctct ccttcttctc tcgagccttt
     4801 ccatatcggt ctcgctcacg ggctggaccc tgaagagctt ttcgcagacg tcgtaagaga
     4861 ggcggtttat gagggatgag aacatatcga acccctcgcg tttgtattcg tggagggggt
     4921 ccctctgcgc gtaacccctg agccctatcc cctctctcag gtggtccatg ttgagcaggt
     4981 ggtctttcca taggtaatcg agggtctgga gcattatgta tctctcgatc tgcctcatgg
     5041 tctcaggcgt tatctcgctt tccttcgttt cataaaactc gtggactttc tttcgtatat
     5101 gctcgatcgc gacttccctt ccgttcacgg ttt
//
LOCUS       DASSJH010000290         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1477883, whole genome shotgun sequence.
ACCESSION   DASSJH010000290 DASSJH010000000
VERSION     DASSJH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477883"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFJ67_06280"
     CDS             <1..580
                     /locus_tag="VFJ67_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006808359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290030.1"
                     /translation="WDSRSEFYNDFGSPRMVSLRKFMLDTVALQSRFIAERMEKSLPK
                     MLESAPPRSRENIRKQFYRVAGSPMGLYLLVDYVNFKGEGTSPAERYKGEGWGLLQVL
                     EIMKGDEKGPSALEEFARSAETLLERRVRNSPPERNEKRFLPGWKKRIGTYGPGNMES
                     YVKGIEYKTDEAVSPEDKIKALYRKLLCGLIY"
     gene            682..1101
                     /gene="ruvX"
                     /locus_tag="VFJ67_06285"
     CDS             682..1101
                     /gene="ruvX"
                     /locus_tag="VFJ67_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET7290031.1"
                     /translation="MRILALDVGTKTIGVAVSDEQGITANGVATIKRKDTEFDMAELG
                     RIIGRYAPSELLVGIPYRSDGSVGKRGEEIMRFAETLNKKFGIPVKYWDESYSTVDAE
                     RFLISADVGRKKRKKVIDKMAAVYILAEYLESKRPVK"
     gene            1117..1908
                     /locus_tag="VFJ67_06290"
     CDS             1117..1908
                     /locus_tag="VFJ67_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7290032.1"
                     /translation="MPYASANGMNIYYEIHGDGYPVVMIGGLGSQIESWATQVPIYSK
                     HFKVVVFDNRGAGRSEKPEPGYSTEDMADDAAALMDALGIGNAHVVGKSMGGMIGQWL
                     AIKYPGKVRKLVMGCSSASRDEVGNLILRTGRDIASKVGMKAVWIMALYLGYTREYIE
                     KNIGSLGGVIDSIPENPDALRGYIGQSYAVEGHDTTQLLLGIKAPTLVMMGENDMTTS
                     PRKTRELASLIPGSELKAFQEVGHGFWRERQEEADKLVLDFLLGG"
     gene            complement(1911..>2375)
                     /locus_tag="VFJ67_06295"
     CDS             complement(1911..>2375)
                     /locus_tag="VFJ67_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7290033.1"
                     /translation="FDVVGMGRIVSSETFEDGRINIVLYGLKRVKILEIMKEIPYRVA
                     RVEIVENARCTSEEILRSRIEDLITKWNFNLDEKRKSHRININTRLPLGSLTDALATL
                     IFSNVFEKQSLLEEPSVQKRAEIIIQDLQTRLDIISITSRKRNEIIDKRGLN"
BASE COUNT          567 a          590 c          704 g          514 t
ORIGIN      
        1 gtgggattca cgcagtgaat tttataacga cttcggctcc ccgcgcatgg tatcgctcag
       61 aaaattcatg ctcgatacgg tagcgctcca gtcccggttc attgcggaga ggatggagaa
      121 aagcctgccg aagatgctgg aatcggcccc acctcggtcg agggaaaata tcagaaaaca
      181 gttctacagg gtagcgggct ccccgatggg cctttatctt ctcgtcgact acgtgaattt
      241 caagggggaa ggcacgtctc ccgccgagag atacaagggg gagggctggg ggctattgca
      301 ggtgctggag ataatgaagg gggacgagaa ggggccttcg gcccttgagg aattcgcccg
      361 ctcggccgag accttgctcg agaggcgcgt tcgcaactcg cctcctgaaa ggaacgagaa
      421 gagatttctc cccggatgga agaaaaggat cggtacttac gggcccggga atatggagtc
      481 ctatgtgaag ggcatagaat ataagacgga cgaggccgta tcgcctgagg ataaaataaa
      541 ggctctgtac aggaagctcc tctgcggatt gatctattga cagggactag acttttttat
      601 tctctgacga tggcgaatgt cgggctccag gccgcaagcg taattccgtc gcggcgttat
      661 ataatgttaa actgacaggt tatgaggata cttgccctcg acgtaggcac gaagacgatc
      721 ggagtcgcgg tcagcgacga gcaaggtata acggcaaacg gtgtggctac gataaagaga
      781 aaggacacgg aattcgatat ggcggagctc ggccggatta tcgggcgcta tgcgccttcc
      841 gagctactcg tgggtatccc ctacaggagc gacgggagcg tcgggaaaag gggagaggag
      901 ataatgaggt tcgccgagac attgaataaa aaattcggaa tcccggttaa atactgggat
      961 gagagctact cgaccgtgga cgcagagagg tttctcatta gtgccgacgt cggacggaag
     1021 aagagaaaga aggtgataga caaaatggcc gccgtgtata tactagccga atacctggag
     1081 tcgaagaggc ccgtaaaata aggagttccg caggtaatgc cttacgcaag cgctaacggt
     1141 atgaacatat actacgagat ccacggcgac ggataccccg ttgtcatgat cggaggtctc
     1201 gggagccaga tcgagagctg ggctacccag gtgcccattt actcgaaaca tttcaaggtc
     1261 gtcgtattcg acaaccgcgg cgccgggagg tcggagaaac ccgaacccgg ttattcgaca
     1321 gaggacatgg ccgacgatgc ggccgcgctc atggatgcgc tcggcatcgg gaacgcgcac
     1381 gtcgtgggta aatccatggg cgggatgata ggccagtggc tcgcgatcaa gtatcccggg
     1441 aaggtccgga agctcgtcat gggctgctcc tccgcatcga gagacgaggt cgggaacctg
     1501 atactcagga caggcaggga catagcttcc aaggtcggca tgaaggccgt ctggataatg
     1561 gctctttacc tcggttatac gagggaatac atagagaaga atatcggctc gctcggaggc
     1621 gttatcgact ccataccgga aaacccggat gctctcaggg ggtacatagg tcagagctat
     1681 gcggtcgagg gacacgacac cacacaactg ctgctcggga tcaaagcccc gacgctcgtc
     1741 atgatgggcg agaacgacat gactacatcg ccccggaaga caagagagct cgcttcgttg
     1801 attccgggct cggaattaaa ggcgtttcaa gaagtgggtc acggtttctg gagggagaga
     1861 caggaagagg ccgacaagct cgtgctcgac tttctcctcg gcggctgacc tcagttgagg
     1921 cctctcttgt ctattatctc attcctcttt ctcgacgtga ttgaaattat gtcgagcctc
     1981 gtctgcaggt cctgaattat tatctctgcc ctcttctgca cgctcggctc ctccagcagc
     2041 gactgctttt cgaagacgtt cgagaagata agggttgcca gtgcgtccgt gaggcttccg
     2101 agcggcagcc tggtgttgat attgatccgg tgggatttgc gcttctcatc gaggttaaaa
     2161 ttccacttcg ttatgaggtc ttcgatcctg cttctgagta tctcttcgct agtgcatcgc
     2221 gcgttctcga cgatctcgac cctcgcgacc ctgtacggta tctctttcat tatctcgaga
     2281 atcttcaccc ttttcaggcc gtagaggact atatttattc tgccgtcctc gaaggtctcc
     2341 gagctcacta tcctccccat cccgacgacg tcgaa
//
LOCUS       DASSJH010000291         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1148565, whole genome shotgun sequence.
ACCESSION   DASSJH010000291 DASSJH010000000
VERSION     DASSJH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148565"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1594)
                     /gene="dnaB"
                     /locus_tag="VFJ67_06300"
     CDS             complement(236..1594)
                     /gene="dnaB"
                     /locus_tag="VFJ67_06300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET7290034.1"
                     /translation="MEMLTRQLPQNLEAEQAVLGAILIESSAINQVMEILTADDFYKD
                     AHRKIYNAMIDLDRDNKPIDLLTLFDYLKGNGNLLEEVGESSYLTYLTEIVPATENVS
                     YYAKLVKEKSIIRNLVMAASDIAHQGNEGVSDIDEFIDSAEHKILDIAQNKIKPSFYD
                     SRDLAAKALDIIEQLHARKEMVTGIPTGFERLDYMTSGLQEADLVIVAARPGLGKTSL
                     CLNIAAHAAIDFGSSVGIFSLEMTKEQLMLRMLSMKALVSYSNIRSGYIKDEDLESLV
                     KAAEQFSHAKLYIDDTPAISVLEIRAKARRQKRDKGLDLIIVDYLQLMRGTRRTETRE
                     REIAEISGSLKALAKELRVPVIAVSQLSRQTEARSDRRPQLADLRESGAIEQDADLVL
                     FIHRADAYRKTPEEKDGIAELIIGKQRNGPTGTVKLTFLDSSRGVPTFKDLSDEYVDD
                     FE"
     gene            complement(1680..>2334)
                     /locus_tag="VFJ67_06305"
     CDS             complement(1680..>2334)
                     /locus_tag="VFJ67_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7290035.1"
                     /translation="IDKYETDMVICLGDIVGYGANPNECVEIIKKRQIKSLTGNHDKA
                     ACGLTEPADFNHVARHAVLWTRGELTRANRDYLRGLPEEGLVDGFMIVHGAPSDPDRY
                     ILSEYDAEEEFPLMGENKLCFFGHTHVGVLYSLSGGDVEVSRAERVRLRRGTSYLVNP
                     GSVGQPRDRDPRASFLIFDDCGDIEFRRVEYPIEAAQKKIIERGLDRFLAERLSLGY"
BASE COUNT          423 a          678 c          609 g          624 t
ORIGIN      
        1 cttcaatatg ttctttgaaa gtaagaagaa ggatttcgcc tgggatttct actggacctc
       61 ggtaagggag aggctcaagg agatactcga caggttccgc gcgtggcagg gagacgaagc
      121 gctctttcac agggaagtga agtcaaaggc tgtggtcgtt cctatgctgg tttagaattc
      181 attattacgt ctggttcccc tcataccccg ataatccaag ctggttagcg cctgatcatt
      241 cgaaatcgtc cacgtattca tcggacaggt ctttgaatgt cgggacgccc ctcgagctgt
      301 ctaggaacgt gagcttcact gtccccgtcg ggccgttcct ctgcttacct attataagct
      361 ccgctatgcc gtccttttcc tcgggcgttt tcctgtaagc gtccgccctg tgtatgaata
      421 gcacgaggtc cgcgtcctgc tctatcgctc cgctctccct gaggtcggcc agctgagggc
      481 gtctgtctga gcgcgcctcc gtctgcctgc tcagctgcga taccgctatc accggcactc
      541 tcagctcctt cgcgagcgcc tttagagagc ccgagatctc cgctatctcc cgttccctcg
      601 tctccgtcct cctcgtgccg cgcatcagct ggaggtagtc gacgatgatg agatcgagtc
      661 ccttgtccct cttctgcctt ctcgccttgg cgcgtatctc taggacgctt atggcaggcg
      721 tatcgtcgat gtagagcttc gcatggctga actgctcggc ggccttgacc agactctcca
      781 ggtcttcgtc ctttatatat ccgctcctta tattcgaata gctgacgagg gctttcatgg
      841 acagcatcct tagcatgagt tgttctttcg tcatttcgag cgagaatatg cccacggacg
      901 agccgaagtc tatggccgcg tgcgcggcta tgttcaggca cagggatgtc ttcccgagcc
      961 cgggtctggc ggccacaatt acgaggtctg cctcctggag ccctgaagtc atgtagtcga
     1021 gcctctcgaa ccccgtgggt atcccagtta ccatctcctt cctggcgtgg agctgctcta
     1081 tgatgtcgag cgccttggcc gcgaggtccc tcgaatcgta gaagctgggc ttgatcttgt
     1141 tctgcgctat gtcgagtatc ttgtgctcgg cactgtcaat gaactcgtct atgtcggaga
     1201 cgccttcgtt accctggtgt gcgatgtcgc tcgccgccat tacgaggttc cgtattatgg
     1261 atttctcctt gaccagcttg gcgtagtagc tcacgttctc ggtcgcggga actatttcgg
     1321 tcaggtaagt gaggtagctg ctctcgccca cctcttcgag caggttcccg ttccccttca
     1381 ggtagtcgaa gagcgtgagc aggtcgatgg gcttattgtc cctgtcgagg tcaatcatag
     1441 cgttgtagat ctttctgtgg gcatctttgt agaaatcgtc ggccgtgagt atctccatca
     1501 cctggtttat cgccgagctt tctatgagga ttgcgcccag aacggcctgt tcggcttcga
     1561 ggttctgggg aagttggcgc gtgagcatct ccatgactat ctatattaaa tcacttgttc
     1621 cgtcgttttc aacacgggag gcttaaaaag aaatcattgg gacggcttac gaagcggctt
     1681 cagtatccga gcgacagtct ttcggcgaga aacctgtcga gtcccctctc tattatcttt
     1741 ttctgagccg cttctatcgg atactcgacc cgcctgaatt caatgtcgcc gcaatcatca
     1801 aatatgagga acgaagcccg cgggtcccgg tcgcgcggct gcccgacgct ccccggattg
     1861 acgagataac tcgttcccct cctcaaccgg acgcgttccg ctctcgagac ttcaacatct
     1921 ccccccgaaa gggagtagag gactcccacg tgcgtgtgtc cgaagaagca gagtttattt
     1981 tcacccatca gcggaaactc ctcctccgcg tcgtattccg acagaatgta tctatcgggg
     2041 tcggacggcg ctccgtggac tatcataaaa ccgtcgacga gcccttcctc gggcaggccc
     2101 ctcagataat ccctgtttgc ccgtgtgagc tccccccttg tccagagcac ggcgtgcctc
     2161 gcaacatggt tgaagtccgc gggctccgta aggccgcacg ccgccttatc gtgattgccg
     2221 gtcaacgact ttatttgcct tttctttatg atttcgacgc attcattggg gtttgccccg
     2281 tagcccacta tatctccgag gcagataacc atgtcagttt cgtatttgtc gatt
//
LOCUS       DASSJH010000292         7397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1478109, whole genome shotgun sequence.
ACCESSION   DASSJH010000292 DASSJH010000000
VERSION     DASSJH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478109"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFJ67_06310"
     CDS             <1..430
                     /locus_tag="VFJ67_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290036.1"
                     /translation="KWGMTGDEVKAAFGKDIKVHKAREDAKEGLYSDLELTGITIGGE
                     PFRASLWMDKATKKLSKIVFVPKGETEGYARAQTFIKVEEDLVSKYGDPDKEETSNDP
                     GTSAERKWEFPSTLIELSYLRIEGTELLLLVFSENTKSSK"
     gene            complement(459..1223)
                     /locus_tag="VFJ67_06315"
     CDS             complement(459..1223)
                     /locus_tag="VFJ67_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290037.1"
                     /translation="MKAVILSIIIFTCIFGGAVLGIFLRAILPERHLTEDSRNMVTIG
                     MGIVATMAAIAVGLMIQGAQISFSSQRSDLIDMSAKIVFLDKLLADYGPEAGDARTAL
                     RSTLERTINQFWPKDGSEGAEIEPPVSKSETLYYQILFLKPDNDARRAMRDEALSLTY
                     DLSLARGTIVMGQSRSIPGVFLIVLGIILFWFVAIFFSFGLYAPTNGTVVATLIISAL
                     AVTLALFMIMELNRPFDGLLRMPSEPLVEALQHIGK"
     gene            1417..2940
                     /locus_tag="VFJ67_06320"
     CDS             1417..2940
                     /locus_tag="VFJ67_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET7290038.1"
                     /translation="MKREHSHKWLTLIAMTGSLSMIFVDQTVVSVALPKIQSDLAVSQ
                     TGLEWIVNAYVLALAVTVALGGKLGDIFGRVRAFVAGVITFALASALCGLAPNEGVII
                     TARALQGLGAALMLPSSAAIVIESFDIRERGRAMAIYVGVAQAFLALGPLLGGFLIEF
                     LSWRWVFWINLPVGALAMIMTYISKPAGLESAGGGVRPVYALLLILGMFAFVFGLQQG
                     HEWGWSSPVTLGFLASGLVLLEIFSFIQIKSDDPLIQLRLFRNPAFAIDCALLFCIQF
                     AMISLIVFGAIFLQKNLGFSPLQAGLGIMPIILSVVVMSQVSGRLFDRVGVKVPALTG
                     AFLIAAGFFLQAPFLEGGDFLKILPGMILLGFGIGFVMVPTSTDALNRAGSEYRGQAS
                     GVVQTMRQIGATVGLASIGGLVATVQKGEVSKIISGFGGPDSVKAGLEPLIDGSLKGQ
                     IDAIRELTAISPALMPELHTAVARSIAAGYYFAGSVVLAAFFVALIFMKRGTQYEDG"
     gene            3156..3599
                     /locus_tag="VFJ67_06325"
     CDS             3156..3599
                     /locus_tag="VFJ67_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290039.1"
                     /translation="MFDRYFSGLRLSSAAVALLLIAAGLYFGACRSDKGEKTEKTGTE
                     SSAVATSAPSSTETPAGQAGEGDKKSAEGLPPEITEPCSGKNEGDPCSVMITGGAEIQ
                     GTCAMTRKNILGCKPIPVIGEKKPAPTSMPPNPNENMDKTQDKSE"
     gene            3858..6509
                     /locus_tag="VFJ67_06330"
     CDS             3858..6509
                     /locus_tag="VFJ67_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290040.1"
                     /translation="MAHARTLIFLAAALIAGLFLGNRINAFLSISIKDKDLEGDSSAV
                     RADLPPLPFRMVDLGGVGIEADAGSWGRDYSHNTRRFDRVFLASEPFVDEAEADRVFD
                     EFREYADRMSELGFNAIEFQAFLELVDFDTLGSGYDVYPKGSVERKRHEALRSFYARF
                     FDYAHQKGLRVILSTDMVALTPPLKRYLSGMPGGMDVESAGFWRVYSEGLAELFGDMP
                     SVDGLMIRIGEAGPLYNRAGWDYTSELMVRSVASVKAMLGSLTKTAEEHGKYIIFRTW
                     SVGVGEVGNMHTDPEVYERVLGDIYSDNLIISTKLTKGDFWNSVPFNPTLYSGRHKRI
                     VELQARREFEAFNVIPNYVAPDYQEALRGFMERNPNVVGVWVWTQAGGPIRQGPMMIY
                     PFHGHWLWTDANVYSTAMIAAHPGEPVGKWTREWVRNTFGGGREVEDGLTELLDMSHG
                     TAVKGLTIPQFARKEVTGVGMEIPPVIYSYWDLMESSTSVMSLVYEASRGELGDAVRD
                     GFAAVSEVGRMKEILASVEGKIEKGREWIPGMEASLDYEENLFSTLAWHKKFMLGFYS
                     WLDKGGVEREREWKEAAGEYKKARAVHMERYQGSLDFPAYNFVMADNGVKQAERTDYA
                     VWTSRALLAALFLFAALVILKGRRAGSGIGTPLFLSAFTFGVLEAFTSFSAPLFVLTT
                     GLPVLVYFFGLRFTVFGARGSGGFAYSLLPALAFLGLILAVTSVRGPYYFWFNVWTSS
                     FFRTALASSAVILLLAHYYMVYSYAREALGSRRLAGRMFFLTGSLVALYGLVYIVFGI
                     DLTLGRINDEILMMPVMVSRVLGFSTHLDFLVVINETAVKAGVVIAAAGLLMAGFGWR
                     KLFSRGGDTPGEKKHADNAVPVRIRKP"
     gene            complement(6619..6894)
                     /locus_tag="VFJ67_06335"
     CDS             complement(6619..6894)
                     /locus_tag="VFJ67_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290041.1"
                     /translation="MKKLIVAGTLLAVLAFPLVSKAEVNAFGVQLPVGNYSAESSAKG
                     GYVAQSLSDTFHVQTLSVSETPKQATDTVNKNVYTVYGIHLSGNDVI"
BASE COUNT         1553 a         2016 c         2257 g         1571 t
ORIGIN      
        1 caagtggggc atgaccgggg acgaggtaaa ggccgcattc ggaaaggaca tcaaggtgca
       61 caaagccagg gaggatgcga aggagggcct ttatagcgac ctcgagctca cgggaatcac
      121 gatcggcggc gagcctttca gggcgagcct gtggatggac aaggcaacca aaaaattatc
      181 gaagatcgtg ttcgtgccca aaggcgagac cgaggggtac gcccgagccc agacgttcat
      241 aaaggtcgag gaagacctcg tctcgaaata cggcgacccc gacaaggaag agacctcgaa
      301 cgaccccggc acgtccgcgg agcggaagtg ggagtttccc tcgacactta tagaattatc
      361 ctacctgcgg atagagggta cggagctgct tctcctcgtt ttctctgaaa acacgaagtc
      421 ctcaaaataa aagacggcta acttctatcg gcttggcgtt atttccctat gtgctgcagc
      481 gcttcaacga gcggctcgct cggcatgcgc agcagtccgt cgaacggcct gttaagctcc
      541 attatcataa agagcgcgag cgtgaccgcc aacgcggata ttatgagcgt agcgacgacc
      601 gttccgtttg tcggggcgta gagcccgaag ctgaagaata tggcgacgaa ccagaagagg
      661 attattccga gcacgataag gaatacgccg ggaatagatc tgctctgccc cattacgata
      721 gtgcctcgcg ccagcgagag gtcgtatgta agcgaaagcg cctcgtcgcg catcgcgcgc
      781 cgtgcgtcgt tgtccggttt gaggaacagg atttgataat acagggtttc ggatttggat
      841 acgggcggct ctatctccgc gccttcagag ccgtccttgg gccagaactg gtttatagtg
      901 cgttcgaggg tgctccgaag agcagttctg gcgtctcccg cctcaggccc gtagtcggcg
      961 agaagcttgt cgaggaagac aatcttggcc gacatgtcta tcaggtcgct gcgctggctg
     1021 ctgaacgaga tctgcgcccc ctgtatcatg agcccgaccg cgatcgccgc catggtggcg
     1081 acaattccca tgccgatcgt gaccatgttc ctcgaatcct cggtcaggtg acgctcgggc
     1141 agaatcgctc ggaggaatat acccaggaca gctcccccga aaatgcatgt aaaaatgatt
     1201 atactgagga ttacggcttt caagacgcac ctcccggcgg aatccgcccc gcttccattc
     1261 ccgccgatat agacgtagtt ataaagcaat gcgcggaaaa attcaataaa tcctctctct
     1321 ccgtccccgg ccgtaccggc gggttgacat ttaatccgcc gtaattacat ttaccgaacc
     1381 cgcgcctgac gagcccgagc gccccgactc tgtcccatga aaagagaaca ttcccacaag
     1441 tggctgacac tcatcgcgat gacgggctcg ctctcgatga tcttcgtcga ccagacggtc
     1501 gtgagcgtgg cgctccctaa aatacagtct gacctggccg tatcccagac cgggctcgaa
     1561 tggatcgtga acgcgtacgt gctcgcgctc gcggtcacgg tcgcgctggg cgggaagctc
     1621 ggggacatat tcgggagggt gcgcgcgttc gtcgcgggcg tgatcacgtt cgccctggcc
     1681 tccgccctct gcgggctcgc cccgaatgaa ggcgtcataa tcaccgcccg agcgcttcag
     1741 ggcctgggcg cggccctcat gctcccctcc tcggccgcca tagtcataga atctttcgac
     1801 ataagggaga gggggagggc gatggcgata tatgtgggcg tggcgcaggc gttcctcgct
     1861 ctcggccctc tcctgggcgg gttcctcatc gagttcctct cctggcggtg ggtgttctgg
     1921 ataaacctcc ccgtgggggc gctcgccatg ataatgacct atatatccaa gcccgcgggc
     1981 ctggaatcgg ccggaggagg ggtgcgcccc gtctatgcac tcctactgat actaggcatg
     2041 ttcgcattcg tcttcgggct ccagcagggg cacgagtggg ggtggtcttc gcccgtgaca
     2101 ctcggcttcc tcgcttcggg gctcgtgctg ctggagatat tttcgttcat acagataaag
     2161 agcgacgacc cgctcataca gctccggctc ttcaggaacc cggccttcgc gatagactgc
     2221 gcgctgctgt tctgcatcca gttcgccatg ataagcctca tagtcttcgg cgccatattc
     2281 ctccagaaga acctcgggtt ctcgccgctt caggcggggc tcgggataat gcccatcatc
     2341 ctctcggtcg tggtaatgtc gcaggtgtcg ggccgcctct tcgacagggt cggcgtgaag
     2401 gtcccggcgc tgacgggcgc gttcctcata gccgctgggt tcttcctcca ggcgccattc
     2461 ctcgaaggcg gcgatttcct gaagattctg cccggaatga tacttctcgg cttcggcata
     2521 gggttcgtga tggtgccgac gagcacggac gcccttaacc gcgccgggag cgagtacagg
     2581 gggcaggcct ccggagtcgt gcagacgatg agacagatag gggcgacggt cgggctcgct
     2641 tccataggcg ggctcgtggc gacagtgcag aagggggagg tctcgaaaat aataagcggc
     2701 ttcggcggcc cggacagcgt gaaagccggt ctcgagcccc tcattgacgg ctctctcaag
     2761 ggccagatcg acgccataag ggagctcacg gccatatccc ccgcactgat gccggagctt
     2821 cacacggccg tcgccaggag catagccgcg ggctattact tcgcgggctc ggtcgtgctc
     2881 gccgccttct tcgtcgccct catattcatg aagcggggga ctcagtacga ggacgggtga
     2941 tggtaccccc tcacccttca ccctctccct caggggggga gagggaagaa aaactcggaa
     3001 tagcgacgcc gaaaatttat gaggaaattc ggtataaaac cctatagact ataagtgttg
     3061 ctaatggtgt ctcctgacgg actagtgaaa aacttgagta ataaatccgg gaaaacaggt
     3121 aaaatttcat caccaccaca acgggaggtt catatatgtt cgatagatat ttttcgggtt
     3181 tgaggctgtc atcggcagcc gtggcccttt tgctcatagc ggccgggctg tatttcggtg
     3241 cgtgccgttc cgacaagggg gagaagacgg agaagaccgg gacggagtcg tccgcggtcg
     3301 cgacatccgc gccgtcgtcc acggagacgc cggccgggca agcgggcgaa ggcgataaga
     3361 agagcgccga aggtcttccg cccgagatca ccgagccgtg cagcggaaag aacgagggcg
     3421 acccgtgctc cgttatgatc acgggcgggg ccgagataca ggggacgtgc gctatgacga
     3481 ggaagaatat actcggatgc aagcccatac ccgtaatcgg ggagaaaaaa ccggctccca
     3541 cctctatgcc gcctaacccg aatgagaata tggataagac gcaggacaag agcgaataac
     3601 ggagacggat ccgcatctcc gcttcctctg attaagaagg atatgaatga aaataatccc
     3661 ggtccgggcg cgcgttttaa tgaagactcg cgcctccggg gattatgacc gtatccccga
     3721 ttgttaagat atttatcata cgcgccgtac gtctgtataa ttaactcctg ccccggattc
     3781 atctccgctt aactgcggat taacgcggtc ttaataaatt ttggccggaa ccgcgggaag
     3841 ccgggcgagg ggacctgatg gcgcacgcgc gcacgctcat ctttctggcg gccgcactca
     3901 tagcgggcct gttcctcggg aacaggataa acgcattcct ttccatatcc ataaaggata
     3961 aggacctcga aggggactcg tccgccgtac gggcagacct tccccctctg ccattcagga
     4021 tggtcgatct cggcggggtc ggcatagagg ccgacgccgg gagctggggc agggactatt
     4081 cccacaatac gaggcgcttc gaccgcgtat tcctcgcctc cgagccgttc gtcgatgagg
     4141 ccgaagctga ccgcgtcttc gatgagttcc gcgaatacgc cgacaggatg tcggagctcg
     4201 gcttcaacgc catagagttc caggcgttcc tcgagctggt cgatttcgac acgctgggca
     4261 gcgggtacga cgtatatccc aaagggagcg tcgagaggaa aaggcacgag gcgttaaggt
     4321 ctttctacgc gcggttcttc gactacgctc atcagaaggg cctccgcgtg atactcagca
     4381 cggacatggt cgcgcttacg ccgcctctca aacgttacct gagcggcatg ccgggcggga
     4441 tggacgtcga gagcgccggg ttctggagag tctattcgga agggctcgcc gagctcttcg
     4501 gcgatatgcc ctctgtggac ggcctcatga taaggatagg ggaagcgggg cctctctata
     4561 accgcgcggg ctgggactac acgagcgagc tcatggtgag gagcgttgca tccgtgaagg
     4621 cgatgctcgg ctcactgacg aagacggcgg aggagcacgg caagtacata attttccgca
     4681 cctggtctgt cggcgtgggc gaggtgggga acatgcacac cgaccccgaa gtttacgagc
     4741 gcgtgctcgg cgatatatat tcagacaatc tcataatctc gaccaagctg acgaaggggg
     4801 atttctggaa cagcgtgcct tttaatccga ccctttactc gggacggcac aagcgcatag
     4861 tggagctcca ggcgcggagg gagttcgagg cgttcaacgt gatacccaac tatgtcgccc
     4921 ccgattatca ggaagcgctc cgcggcttca tggagcgtaa cccgaacgtg gtcggcgtgt
     4981 gggtgtggac ccaggcgggc gggccgataa ggcaggggcc gatgatgata taccccttcc
     5041 acgggcactg gctctggacc gacgcaaacg tgtactcaac ggcgatgatc gcagctcatc
     5101 ccggcgagcc cgtcggtaaa tggacgcgcg agtgggtgag gaatacgttc gggggaggca
     5161 gggaggtcga ggacgggctc acggagctcc tcgacatgtc gcacggcacg gccgtgaagg
     5221 gactcaccat accgcagttc gcccgtaaag aggtgacggg cgtggggatg gagataccgc
     5281 ccgtcatata cagctactgg gacctaatgg agagctcgac ctccgtgatg agcctcgttt
     5341 acgaggcgtc gaggggcgag ctcggcgacg ccgtaaggga cgggttcgca gccgtctctg
     5401 aagtcgggcg catgaaggag atactcgcgt cagtcgaggg caagattgaa aagggaaggg
     5461 aatggatacc cggcatggag gcctctctcg attacgagga aaaccttttt tcgaccctgg
     5521 cctggcacaa gaagttcatg cttgggttct actcgtggct cgacaaggga ggggtagaga
     5581 gggagaggga atggaaggag gcggccgggg agtataagaa ggccagggcc gtgcacatgg
     5641 agaggtatca ggggagcctc gatttcccgg cttacaactt cgtcatggcc gataacggcg
     5701 tcaaacaggc ggagaggacg gattatgcgg tatggacatc gagggcgctc ctggctgcgc
     5761 tctttctttt cgccgcgctc gtcatactga agggcaggcg cgcgggctcc gggatcggga
     5821 cgccgctatt cctttcggcg ttcaccttcg gggtgctcga ggcgttcacg tcgttcagcg
     5881 ctccgctttt cgtccttacg acgggactgc ccgtactcgt ctatttcttc ggtctcaggt
     5941 tcactgtgtt cggcgcgcgc ggaagcggag gcttcgccta ctcgctcctg ccggcgctcg
     6001 cgttcctcgg tttaatactc gcggtcacgt cagtgagggg gccttattat ttctggttca
     6061 acgtgtggac gagcagtttt ttccggacgg cgctcgcttc ctcggccgtc atattacttc
     6121 tggcgcacta ctacatggtt tactcatacg cgagggaggc gctcggctct cggcggctcg
     6181 cgggcaggat gttctttctc acggggtcgc tcgtcgcgct ctacgggctc gtgtacatag
     6241 tcttcggcat cgaccttact ctcggcagga taaacgacga gatactgatg atgcctgtca
     6301 tggtatcccg cgtgctcggt ttctcgacgc acctagattt cctcgtggtc ataaacgaga
     6361 cggcggtcaa ggcgggcgtg gtcatagcgg ccgcggggct gctgatggcc gggttcgggt
     6421 ggaggaagtt attttcacgc gggggcgata cgcctgggga aaagaaacac gcggataacg
     6481 ccgtgcctgt tagaatccga aagccttaat aatcacaatc tgctcaatag ctaaaccgtc
     6541 ttcattaacg aagaagcccg gtcagagccg ggcttccttt tcatcctgtc atcgatttgc
     6601 gcgcgctgcc cgcatccgct atatgacatc gtttccgctc agatggatgc cgtataccgt
     6661 gtagacgttc ttgttcacgg tgtcagtggc ctgcttggga gtctcgctga ccgaaagcgt
     6721 ctggacatgg aacgtgtcgc taaggctctg tgcgacgtat ccgcctttgg cgctgctctc
     6781 ggcgctgtaa ttacctaccg ggagctgcac tccgaacgcg ttcacttccg ctttcgatac
     6841 gagcgggaaa gcgaggaccg ccaataatgt acctgcaaca attaacttct tcattttact
     6901 gcctccttaa aaaatgtatt tgactgttta gttgttattg caacgaggat ggattcagaa
     6961 gttgataaat aatattaatc caatgatcac agtgttatac ccgccgcaat aactgattat
     7021 gtgaatagat acggatatat cgggaaaata gatttattta ttttaatgtg ccggtttgca
     7081 cagtgacggt ttaagtcgtg ataaaccccg caggcgttga ccgattatca tgagatgcat
     7141 atgcaaaaat aaagattcgg attttgtacg ccggtcttta tttgagcgga tggatgtatg
     7201 gagagacgca acgataacgt tatcgagtga tatcaatttt cgtatacggg gtacttccgg
     7261 tgtacttacg gggcactttt ggttcaggcg gagagttttc tgcgcgtgcg cctgatatgg
     7321 tcatcgagcg tgacgggtgt gaggacgggg tctctctcaa ggtcgggcgt gaggctgaat
     7381 tcgtagtcct cttctcg
//
LOCUS       DASSJH010000293         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1148637, whole genome shotgun sequence.
ACCESSION   DASSJH010000293 DASSJH010000000
VERSION     DASSJH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148637"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..557
                     /locus_tag="VFJ67_06340"
     CDS             105..557
                     /locus_tag="VFJ67_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7290042.1"
                     /translation="MARIRMEFPESPVYSTDINLRISDINYGGHLGHDSILSLTHEAR
                     VRFFRHFGFSELDVDGPGIVLSDVAIVYKSESFYGETMKIDVAVCDFVKYGCDLVYRL
                     SEKESGREVAIAKTGIVFMHYAERKVAPVPGKFKKLFSEQSYSLEIQP"
     gene            complement(637..1833)
                     /locus_tag="VFJ67_06345"
     CDS             complement(637..1833)
                     /locus_tag="VFJ67_06345"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7290043.1"
                     /translation="MQKVVISEPLRTAIGAFGGTLKEISPAKLGSTVVKEILDRTGID
                     PKEIDDCIMGNILCAGQGMNISRQVSIASGLPVETPAFTINRVCGSGVQSVALAAQAI
                     KAGDSEAIIAGGTENMNLAPFYLQKARYGYRMGMPKDEIVDGMVYDGLWDIFNDYHMG
                     VTAENLAESYNITKDEQDEFAYKSQMKCKAAQDEGKFKDEIVPIMIPQRRGDPIAFEK
                     DEHPKGDTTLEGLKGLRAVFKVGGTVSAGNASGINDGAAAVLVTSEKKAKELGLKPVA
                     SIISYAVGGVDPSIMGIGPVPAIQKALDKAKLSLDDIDLFELNEAFAAQSLAVLRDLP
                     IPEEKINVNGGAIALGHPIGASGTRILVTLLHEMEKRKKVKRGLAALCIGGGMGIAII
                     VEKEKS"
     gene            2001..3119
                     /locus_tag="VFJ67_06350"
     CDS             2001..3119
                     /locus_tag="VFJ67_06350"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET7290044.1"
                     /translation="MSRLTVGVVFGGRSVEHEVSLLSATSVIRNVDPGKYDVFPIFIE
                     KSGIWRKASVSAWLDEGGIEFFEDSFLSPSLNPGKPVFYEITGKKIVKEHAVDVIFPV
                     LHGTHGEDGAIQGLFELMGLAYVGASVLGSSVGMDKIAMKSLLRDRGLPVIEFLGFYK
                     SEWVAGKEKLRERILKEIGLPCFVKSANLGSSVGITKVTSESELDAAVDYSCRFSESV
                     IVERAVRKPREIEVSVLGNDDPQASCPGEVIPHREFYDYTAKYLEEGTGLIAPAELDE
                     ETSAKLRDFAVRTFRALHCSGMGRVDFLLEEGTNDIFVSEINTIPGFTKISMYPRLWE
                     VSGLSFPELVNRLIELAIERRRQADSLESDYSDIGVKD"
BASE COUNT          745 a          905 c          969 g          838 t
ORIGIN      
        1 cgctgatttc ggggcggctg taaataacgt aataagggtc tcactcgata catgcccgga
       61 gttagaccgg gcaggcttac aataaatcat tagaggaaac tcacatggcc aggataagaa
      121 tggaattccc ggagagccct gtatactcaa ccgatataaa cttgaggata agcgatatca
      181 actacggcgg gcacctcggt cacgactcca tactgtccct cacccacgag gcgagggtga
      241 ggttcttcag gcattttggc ttctcggagc tcgacgtgga cggccccggg atagtccttt
      301 cggacgtcgc catagtatat aaatccgaga gtttctacgg ggagacaatg aaaatagacg
      361 tggccgtttg cgatttcgtc aaatacgggt gcgacctcgt ttacaggctc agcgagaagg
      421 aatcggggag ggaggtcgcc attgccaaga ccgggatcgt gttcatgcat tacgcagaaa
      481 ggaaggtcgc gccggtgccc gggaaattca aaaagctctt cagcgagcag tcatactctc
      541 tcgagatcca gccttgatgt cccaatagcg tatagttcgg tatgacggta agaataaagg
      601 ggagggcttc cgccctcccc cgtggattac ttagagttaa ctcttttcct tctcaactat
      661 aattgcgata cccataccgc ctccgatgca gagtgcggcg agtccgcgtt tcactttctt
      721 tctcttttcc atctcgtgga gcagcgtaac gagtatcctc gtcccggaag cgccgatcgg
      781 gtggccgagc gcgatcgctc cgccgttcac gtttatcttc tcttcgggta tcggcaggtc
      841 cctcagaacg gcgagagact gcgccgcgaa ggcttcgttc aactcgaaga ggtcgatgtc
      901 gtctaacgag agctttgcct tgtcgagcgc cttctggata gcggggacag ggccaatacc
      961 catgatcgag gggtcgacgc cgcccacggc gtaagaaatg atggacgcca cgggttttag
     1021 tcccaattct ttcgctttct tctccgacgt gacgagaact gcggccgcgc cgtcgttgat
     1081 gccggacgcg ttgccggccg agactgtccc gcccactttg aacaccgcgc ggagaccctt
     1141 tagtccttcg agtgtagtgt cgcccttagg gtgctcgtcc ttctcgaaag ctatggggtc
     1201 acccctcctc tgggggatca ttataggtac tatttcgtcc ttgaatttgc cctcgtcctg
     1261 ggcggccttg cacttcatct ggctcttgta cgcgaactcg tcctgctcgt ctttcgttat
     1321 gttgtaggat tcagccaggt tttccgctgt aacacccatg tggtagtcgt tgaaaatgtc
     1381 ccagaggccg tcgtacacca tgccgtccac gatctcgtct ttaggcatgc ccatcctgta
     1441 gccgtaacgc gctttctgaa gatagaacgg ggcgaggttc atgttctcag taccgccggc
     1501 gattatcgct tcggaatccc cggccttaat tgcctgagcc gcgagcgcga ccgactgtac
     1561 gcccgaaccg caaaccctgt taatcgtgaa tgcaggggtt tccaccggca gtcccgatgc
     1621 tatcgagacc tgcctcgaga tgttcatacc ctggcccgcg cacagaatat ttcccataat
     1681 gcagtcgtct atttcctttg gatcgatccc cgtgcggtcg aggatttcct ttactactgt
     1741 ggatcccaac tttgccggac ttatctcttt caatgtgccg ccaaaagctc cgatagcagt
     1801 tcttagcggt tcgctaatta cgactttctg catatttagc ctccttatta tttgttacac
     1861 actattgccg tgtgtggatt ttacattaag gctttttatt tgcaagctag cggggaccct
     1921 gtcttgacaa cgatacgggg tttgggtaac ccgttagtga tttttacctg ataaactatc
     1981 tattcgggag tatagcatac ttgtccagat taaccgtagg agtggtattc ggcgggaggt
     2041 ccgtggagca tgaggtgtcg ctcctttcgg cgacgtcggt aataaggaac gtcgaccccg
     2101 gaaaatacga tgtattccct atatttatag agaagagcgg catatggagg aaggcatcgg
     2161 tctcggcctg gctcgacgaa ggcggcatcg aattctttga agactcgttc ctctccccgt
     2221 ccctcaaccc cggaaaaccc gtattttacg aaataacggg caaaaagatc gttaaagagc
     2281 acgcggtgga cgtgatcttt ccggtcctcc acgggacaca cggggaggac ggcgctatac
     2341 aggggctgtt cgagctgatg ggtctggctt atgtcggcgc ctcggtgctg ggttcttcag
     2401 tgggtatgga caagattgct atgaagtctc ttttgaggga ccgggggctg cccgtcatcg
     2461 aattcctggg attttataaa tccgaatggg tggctggtaa ggagaagctc agggagagga
     2521 tactgaaaga gatcgggcta ccgtgtttcg taaaatcggc caatctcgga tcgagcgtgg
     2581 gcatcacgaa ggtgacgtcg gaaagcgagc tcgacgcggc ggtcgattat tcgtgccgct
     2641 tctcggagag cgtgatcgtc gagcgcgccg tccggaagcc gagggagata gaggtgagcg
     2701 tgctcggtaa cgacgatccc caggcgtcgt gtccggggga ggtgatcccg cacagggaat
     2761 tctacgatta cacggccaag tatctcgaag aggggactgg gcttatcgcc ccggctgagc
     2821 tcgatgagga gacctcggca aagctcaggg acttcgccgt gcgcactttc agggcgcttc
     2881 actgcagcgg catggggagg gtcgatttcc ttctcgaaga agggacgaac gatatattcg
     2941 tgagcgagat aaacacgatc cccggtttca cgaagattag catgtacccg cgcctctggg
     3001 aggtgagcgg gctgagcttc ccggagctcg tcaacaggct catcgagctc gcgatagaga
     3061 ggcggaggca ggcggactct ctcgaatcgg actattcgga catcggcgtg aaggactgaa
     3121 cctgcccgga gctaacctta cggccgtacc ccgccgctca acccgtgtta tccccactaa
     3181 agataaacct accttagata atatacttaa attcatcaaa gccgagcgta tccgattctt
     3241 ggggtatatt ttaaggatat ataagctcca tataccgtac aggctgctag aaggcggtat
     3301 catattgagg ctaatgagtt tcagaatccg tgctgctgtt ccgcgaggtc ttcttatgaa
     3361 agaggtttta ctggctctct tgctgtactt tttattcgga atcctgcccg ccccggccgc
     3421 gcctccggga aaggaagagg gcgtggaggc gactccc
//
LOCUS       DASSJH010000294         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1369543, whole genome shotgun sequence.
ACCESSION   DASSJH010000294 DASSJH010000000
VERSION     DASSJH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369543"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..973
                     /locus_tag="VFJ67_06355"
     CDS             2..973
                     /locus_tag="VFJ67_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7290045.1"
                     /translation="MQETMTVQNGNARELEFGDNNLIKELYGENNDNLKEIEGHYGIK
                     IHSRGGKLNLEGKVEGVDSADRFLREMYGILEKGYTLDPADIELAARIVDEGKIRLAD
                     VFLDTVCVSTRKRIIAPKSIAQKIYIESIRRHDIAFGIGPAGTGKTYLAMAMAVSAFV
                     NKEVNRIVLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLLDALYDMMDYEKASRLLEKG
                     AIEIAPLAFMRGRTLNDAFIILDEAQNTTSMQMKMFLTRLGYNSKAVITGDTTQVDLA
                     ASERSGLLEAELLMKKIEGISFVYFSKNDVVRHPLVRKIIEAYEKNS"
     gene            1026..1475
                     /locus_tag="VFJ67_06360"
     CDS             1026..1475
                     /locus_tag="VFJ67_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290046.1"
                     /translation="MRKFLCILSLVILSLSAACSREWRDPDTALPSQDVSISTILANP
                     DAYDMSGVIVIGKIWRPRVESLGLNEDGVEQVYTVFTIADRMGIGIDVYVNGEAPVND
                     GDYIRVVGLYRKEFQTQGDYFYSRIDAVRLESWSPNLTYWLREFEFD"
     gene            complement(1485..>2598)
                     /locus_tag="VFJ67_06365"
     CDS             complement(1485..>2598)
                     /locus_tag="VFJ67_06365"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET7290047.1"
                     /translation="EGKISVIGNGVVIDPKVLLEELRGLRSAGYRVDSGNLKISDRAH
                     VIMPYHREIDIARESTSQGNGKIGTTGRGIGPVYEDKAARRGIKFSDLIDPESLRERL
                     SSVLDERNKYLEHVLGRKPLRFDDIYNEYKEYGRELKDFSCDVSSLLNKSIDEGKCVL
                     FEGAQGALLDIDLGTYPYVTSSSASSGGACTGTGVSPTRIDAVMGIAKAYATRVGEGP
                     FPSEIEGELGEKLREAGGEYGATTGRSRRCGWFDCVALKYAAMTNGISRLAITKLDVL
                     SGFEKIRICTGYMYRGEMLPGFPSNINILKDCEPIYTEMEGWKEDISSVADFDALPSQ
                     ARNYIRKVEELTGVTIYAVSLGASREKILFLSELFN"
BASE COUNT          568 a          712 c          724 g          594 t
ORIGIN      
        1 tatgcaggaa accatgacgg tccagaacgg gaacgcgagg gagcttgaat tcggcgacaa
       61 caatctcatc aaagagctct acggggagaa taacgacaac ctgaaggaga tcgaggggca
      121 ttacggcata aagatccatt cgaggggagg gaagctgaac ctcgagggta aggttgaggg
      181 agtcgactcg gccgacaggt tcttgaggga gatgtacggc attctggaaa aaggctatac
      241 gctcgacccc gccgacatag agcttgccgc gaggatagtc gacgagggca agattcggct
      301 tgccgatgtg tttctggaca cggtctgcgt ctcgacgagg aagcggatta tagctcccaa
      361 gagcatagcg cagaagatat acatagaatc aatcaggaga cacgatatag cattcggcat
      421 cggcccggcc ggaaccggaa agacgtacct cgctatggca atggccgtgt cggcgttcgt
      481 caacaaggaa gtcaaccgga ttgtgctcac gaggccggct gtcgaggcgg gcgagaagct
      541 cgggttcctt ccgggcgatc tctcgcaaaa ggtggacccg tatctgaggc cgctccttga
      601 cgcgctatac gatatgatgg attacgagaa ggcatcgcgt ctcctcgaga aaggagcgat
      661 cgagatagcc ccgctcgcgt tcatgagggg aaggacgctg aatgacgcct tcataatatt
      721 agacgaggcc cagaacacca cgtcgatgca gatgaagatg tttctcacga ggctcggata
      781 caactcgaaa gccgttatca cgggcgatac cacgcaggtg gacctggcgg cgagcgagcg
      841 gtcgggattg ctcgaggctg aattgctcat gaaaaagata gagggcatct cgtttgttta
      901 tttctccaag aacgacgtcg tgagacaccc gctcgtgagg aaaattatag aagcgtacga
      961 gaaaaattca taagtactgt gttataatta tctgctgtcc ggtatcatta tcggagatat
     1021 ttcatatgcg aaaattcctg tgcatcctct ctcttgtaat tctctccctc tccgcggcgt
     1081 gttcacgcga gtggagggac ccggacaccg cgctcccttc gcaggacgtc agtatttcca
     1141 caatactcgc taaccccgat gcatacgata tgtcgggcgt gatagtgatt ggaaagatat
     1201 ggcggccgag ggtcgaatcc ctgggactga acgaggacgg ggtcgagcag gtttataccg
     1261 tgttcaccat cgcggacaga atgggaatag ggatcgacgt ttacgtcaac ggcgaggccc
     1321 ctgtcaatga cggcgattat atcagggtcg tcggtctcta caggaaagag tttcagacgc
     1381 agggcgatta cttctacagc aggatagacg ccgtgcgcct ggaaagctgg agtcccaacc
     1441 tgacctactg gctcagggaa ttcgaattcg attagcccgg ggaatcaatt aaacaattcc
     1501 gagaggaaga ggatcttctc cctcgacgct ccgagcgata cggcgtatat cgtaacgccc
     1561 gtgagctctt ctacctttct gatgtaattc ctggcctggg agggcagtgc gtcgaagtct
     1621 gcgacggatg aaatatcttc cttccacccc tccatctccg tataaatcgg ttcacagtct
     1681 ttcagtatgt tgatgttcga agggaagccc ggcagcattt cgcccctgta catataccct
     1741 gtgcatatcc tgattttctc gaagcctgac agaacgtcga gcttcgttat cgcgaggcgg
     1801 gagatgccgt tcgtcatcgc ggcgtattta agcgcgacgc agtcgaacca cccgcacctt
     1861 ctcgacctcc ccgtcgtcgc cccgtattca ccccctgcct ccctcagctt ctcgccgagc
     1921 tccccctcga tctcggaggg gaacgggcct tcgccgaccc tcgtcgcgta ggctttggct
     1981 atgcccatca ccgcgtctat ccgcgtcggg ctcactcccg tgccggtgca tgcgcctccc
     2041 gagcttgcgc tcgatgacgt gacgtaggga taggttccga ggtctatatc gaggagcgcc
     2101 ccctgcgcgc cttcgaacag gacgcacttc ccttcgtcga tggatttatt gagcaggctc
     2161 gagacgtcgc aggagaaatc ctttaactcc ctgccgtatt ctttgtattc gttatatatg
     2221 tcgtcgaatc ggaggggctt tctcccgagc acgtgctcta ggtatttgtt cctctcgtcg
     2281 agcacgctcg atagcctctc cctcagggac tcgggatcga tgaggtcgga gaactttatc
     2341 cccctcctcg cggccttgtc ttcgtaaacg gggcctatcc cccttccggt ggtccctatc
     2401 tttccgtttc cttgcgaggt cgattcgcgc gctatgtcta tttccctgtg gtagggcatt
     2461 atgacgtgcg ccctgtcgct tattttcagg ttcccggaat cgaccctgta gcctgctgac
     2521 ctgagtcccc gaagctcttc gaggaggact ttcggatcga tgaccacgcc gttgccgatc
     2581 acggatatct tcccctcc
//
LOCUS       DASSJH010000295         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1148861, whole genome shotgun sequence.
ACCESSION   DASSJH010000295 DASSJH010000000
VERSION     DASSJH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148861"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..550)
                     /locus_tag="VFJ67_06370"
     CDS             complement(287..550)
                     /locus_tag="VFJ67_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290048.1"
                     /translation="MNGTPAAKGYDTSPMSVGDWVITMILLAIPVVGLVMLIYWIVSS
                     TGNVNRRNYCLASLVIAAIIIVLAIIFAVFFGGMAALMGTHTS"
     gene            complement(793..>2860)
                     /locus_tag="VFJ67_06375"
     CDS             complement(793..>2860)
                     /locus_tag="VFJ67_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7290049.1"
                     /translation="ARFTESLIASEKALEEKVSILSKKESYEKIVYSIAAGVHGSVDF
                     DEVLRLTVKGLRQNIKNVDFVTVYFVEGNEAVMKSAYGPDLNLRKKVEKIPYPRGLTW
                     RTIIEGKIRFVPDTEVDDAIGPAGRQSGVRSYISMPIQSVGTTIGCITIASEKVNAFD
                     REELMLLESVAEQIRIALLNAKYVAEIESNEKRLKALVGSVDEIVFEFDEQGTYLGVW
                     TENEELLSRPKEELLGRRVSDLFDEEFSNSVTSAIKRVLKNKKSEVVEFNVVLNGRER
                     CFRARINPIFASGREPDTVSMLARDVTESKALETQLLRSQRLESVGKLAGGIAHDLNN
                     ILQPIMMSIQLLNTRVTDEKSLKWLEILDRSAQRGADLIRQILSFARGLESRKQPFDL
                     KYLVKDVKKILDETFPKLINLNVEIEEDLPSVTGDYTQLNQVLMNLCVNARDAMPEGG
                     ELMIRIRKIARGQDEMPRFIESEVPDFIVIEVSDTGTGIAPEHMDKIFDPFFTTKSSD
                     KGTGLGLSTAYGIIKEHNGFINVESHPGKGSVFTIYLPVGETAGLAEALDELTSNVHA
                     GNGEMILVVDDESMITDMARTILEEYDYKVLVAHNGEEATKIFLTHKDEIYAAIIDMM
                     MPVMGGKATIRTLKKERPALKVIAMSGYQREHELVDLKEAEVDAFLPKPCNSEKLLQT
                     LDEVIH"
BASE COUNT          556 a          828 c          740 g          736 t
ORIGIN      
        1 agaacggatg cgaacgagtg agcattcgga cgccattaca acaaaagcgc ctaagcaaat
       61 caaattccgg ctgaatgttt attaagacca aataggtcag ggaggatttt atcttaatta
      121 aaaaggggaa acttcccgac tgtgcgcaaa gaaacatccc cccacgacct ccgggcggcg
      181 gattccccgc tttcggggta gccgccgctc tagtggccgt ctcgttaagt actctatgat
      241 tgccggaata tcgggtgcct gtgtctcact gccggcgcgg ggatgcctat gacgtatgcg
      301 tccccataag cgcagccatc ccgccgaaga agacggcgaa gataatcgcg agcactatga
      361 taatcgccgc gataactagc gacgcgaggc agtagtttct cctgttgacg ttcccggtgc
      421 tgctcacgat ccagtagata agcatcacga ggccaactac gggtatggcc aggagtatca
      481 tagtgataac ccagtccccc acgctcatgg gggaagtatc gtaacctttt gctgcagggg
      541 ttccgttcat tccattatcc tccttgccga taagcgatta ataatgagta attatagtac
      601 ggtatgaaaa attatgatag gcggaatttt tcattgcggc aggccgctat ttaaaagact
      661 ggaaaaccgg tcggtgtcgg gaatgactcc ttttttagtc ttcgcgaagg agcggagcgg
      721 cttcggcgat ctctctttat aataatgaga ttgccgtgct ccgcgcagcc gcctcgcaat
      781 ggcgcttcgg cattaatgta tcacttcgtc gagcgtctgg aggagcttct cggagttaca
      841 gggcttgggc aggaaggcat cgacctcggc ttccttcaga tccacgagct cgtgctccct
      901 ctggtagccg ctcatcgcta ttacttttaa ggccggcctc tccttcttga gagtgcgtat
      961 cgtcgccttg ccgcccatca cgggcatcat catgtcgatt atcgcggcgt aaatctcgtc
     1021 cttgtgcgtg aggaatattt ttgtcgcctc ctcgccgttg tgagcgacca gcaccttgta
     1081 gtcgtattcc tcgagtattg tcctcgccat gtccgttatc atgctctcgt cgtccactac
     1141 gagtatcatc tcgccgttcc ccgcgtggac gttcgaggtg agctcgtcga gcgcctctgc
     1201 caagcccgcg gtttccccga cgggcagata aatagtgaat accgacccct ttccggggtg
     1261 gctttcgacg tttatgaagc cgttatgctc cttgattatc ccgtatgccg tggacaggcc
     1321 gaggcccgtt cccttgtcgg aagacttcgt cgtgaaaaag gggtcgaata tcttgtccat
     1381 gtgctccggt gcgataccgg tgccggtgtc tgacacctcg atgacgatga agtccgggac
     1441 ctccgactct atgaaccgcg gcatctcgtc ctgccccctc gcgattttcc gtatccttat
     1501 catgagctcg ccgccctcgg gcatcgcgtc cctcgcgttc acgcaaaggt tcatgagcac
     1561 ctggttgagc tgggtatagt ccccggtgac ggacgggagg tcttcttcta tttccacgtt
     1621 caggtttatt aacttcggga acgtctcgtc gagaatcttc tttacgtctt ttacgaggta
     1681 cttcagatcg aacggctgct tgcggctttc gagccccctc gcgaacgaga gtatctgtct
     1741 tatgaggtcg gcccccctct gggcgctgcg gtcgagtatc tcgagccatt tgaggctctt
     1801 ctcgtcagtc accctcgtat tcaggagctg tatggacatc attataggct ggagaatgtt
     1861 gttgagatcg tgcgctatgc ctcccgcgag cttgccgacg ctctctaacc tctgggagcg
     1921 caggagctgg gtctcgagcg ccttgctctc ggtgacgtcc ctcgcgagca tggataccgt
     1981 atcaggctct ctgcccgagg cgaaaatcgg gttgatcctc gccctgaagc acctctccct
     2041 gccgttgagc acgacgttga attccacgac ctcggatttc ttgtttttca gcacccgttt
     2101 tatggcagac gtgacggaat tcgagaactc ctcgtcgaac aagtcggaca cgcgcctgcc
     2161 gagaagctct tccttcggcc tcgagagcag ctcttcgttc tccgtccaga cgcccaggta
     2221 cgtcccttgc tcgtcgaact cgaagactat ttcgtcgacg gaccctacga gagccttaag
     2281 tctcttttcg ttcgattcta tctccgccac gtacttggcg ttgagaagcg cgatacgtat
     2341 ctgctccgcc acggattcga gcagcatgag ctcctcccgg tcgaaagcgt tgaccttttc
     2401 ggacgcgata gtgatgcacc cgatcgttgt tccgaccgac tgtatcggca tcgatatata
     2461 gctcctcacc cccgactgcc tcccggcggg tcctattgca tcgtcgactt ccgtatccgg
     2521 gacgaaacgg atcttcccct cgattattgt cctccacgtg agtcccctcg ggtagggaat
     2581 cttttccact ttcttcctga ggttgagatc aggaccgtaa gccgatttca tcactgcttc
     2641 gttcccctcg acaaaatata cggttacgaa gtccacgttc ttgatgttct gcctgaggcc
     2701 ttttaccgtg agacgcagca cttcgtcgaa gtcgaccgag ccgtggactc ccgcggctat
     2761 ggaatagact atcttctcgt agctctcctt cttggagagg atactcacct tctcttcgag
     2821 cgccttttcc gaagcgatca gggactccgt gaacctcgcg
//
LOCUS       DASSJH010000296         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_722007, whole genome shotgun sequence.
ACCESSION   DASSJH010000296 DASSJH010000000
VERSION     DASSJH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722007"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /gene="dacB"
                     /locus_tag="VFJ67_06380"
     CDS             <1..1065
                     /gene="dacB"
                     /locus_tag="VFJ67_06380"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET7290050.1"
                     /translation="HLGFIVYQLKKRGVNEIKDGITVDDTYFSKTRRAEGWKVEWDDD
                     FYSPPISALSFNYNTIEVKVTGTKSGQRPYVSILPEGSNITIINNAVTSSKKGGVLTT
                     DWRDDQTIVVSGRVRSKASVLLKIPVQNPTILTGNMLKSALEEAGIKVSGPVVEGGVP
                     RWATLFYTHYSDPLSTIITEYLKNSVNIIGENLIKTLAAEFRGVPGSWENGSSVISEF
                     LNNIGIKDGFKVVDGSGLSLLNRVSPQTLTEVLSYAYTNRIIASDFIDSLPIAGVDGT
                     LKKRFRESDVQGRVMAKTGYLNNVRALSGYVFTKQGDVLVFSILDNGLGWKTKEFQSS
                     LLSELVGCCGTGVAGNSTIN"
     gene            1372..2307
                     /gene="gspC"
                     /locus_tag="VFJ67_06385"
     CDS             1372..2307
                     /gene="gspC"
                     /locus_tag="VFJ67_06385"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HET7290051.1"
                     /translation="MLATILKKYVWVINLCLLTGLAYLAAATVNQKIEGMISSPRAAA
                     SQKFLQDERDNKTESTTRHAVSYYRVILTRNIFGITNLSDSNLSSTNQEAAPDTTLNI
                     VLLGTIINKDGSSVAIIKNPDNNKVNGYHSGDTVDIISSDDVKLVEITNCKVVIDRQK
                     TGRETIKCKDLGDVASTAPIAPSGKRMLKRGEGSESQEKSGEDEGIKKVGENEYEVSR
                     ELLEEVLSDPTNIVQQARVIPQEDGLRFFGIRSNSIFWKIGIKNGDTLHKINNVELND
                     VEKALSVFEELRSQSNFTIDFTRAGKEYTYEYNVR"
     gene            2419..>2868
                     /locus_tag="VFJ67_06390"
     CDS             2419..>2868
                     /locus_tag="VFJ67_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7290052.1"
                     /translation="MRALLINILILGFLLTLYVYVNTDHEKTSIKENAAQTTELEDNG
                     STLPVGNEGSSYSYRVEEGDTLSRIAENVHLSVKELKEFNGLTKDELSVGEYIDIPGG
                     KDNNGVEIAAKDDSTPFGDPDNSQTMTQSLSDQEQSPAYNDQGSSDQQ"
BASE COUNT          856 a          685 c          723 g          604 t
ORIGIN      
        1 cacctaggat tcattgtcta tcagcttaaa aaaaggggcg tcaacgagat caaggacgga
       61 atcacggtgg acgataccta tttcagcaaa acccgccggg cagaaggatg gaaagttgaa
      121 tgggacgatg atttctacag ccccccgata agcgcgcttt ccttcaatta caataccatc
      181 gaagtcaagg tcaccgggac caaatccggg cagaggccat acgtgtcgat cctgcccgaa
      241 ggctctaaca tcacgataat aaacaatgcc gtcacgagct cgaagaaggg gggggtgctg
      301 acgacggact ggagagacga tcaaactatc gttgtctcgg gcagagtcag gtcaaaggcg
      361 tccgtgcttc tcaaaatccc ggtgcaaaac ccgaccatcc tcacaggcaa catgctgaaa
      421 agcgccctgg aagaggcggg catcaaggta tcgggccccg tcgttgaagg cggcgtcccc
      481 aggtgggcga cattatttta tacgcattat tccgatccgc tctccactat cattaccgaa
      541 tacctcaaga acagcgtaaa catcataggg gagaacctga taaagaccct cgccgccgag
      601 ttcaggggcg tccccggttc atgggaaaac ggctcttccg tcatctcgga gttcctaaac
      661 aacataggta ttaaagacgg tttcaaggtc gtcgacggct cgggtttgtc actcctcaac
      721 cgcgtctccc cccaaacact gaccgaggtg ctgagctacg cctacacgaa caggatcata
      781 gcgtccgact tcatcgactc ccttccgatc gccggcgtgg acggaacact caagaaaagg
      841 tttagggagt cggatgtcca gggaagggta atggcgaaaa ccggttacct taacaacgta
      901 agagcccttt ccggttacgt ttttacgaaa cagggagacg tcctggtgtt ctcgatcctc
      961 gataacggac tcggatggaa gacgaaggaa ttccagagca gcctgctttc agagctcgtc
     1021 gggtgctgcg gcaccggtgt agccgggaat tcaaccatta attaagggcc aatatatagt
     1081 cccgggaatt gatttttggc aaatcgaaaa gtaaattgta ttaagaggca gggaattcaa
     1141 tccattacca ttgtttattt gtctatcgac cctgaattaa aatccgaggc actccggctc
     1201 cggactcggg tttcagagaa ttaagtgagc gaggtggctg tcgctacagc aaaaaagcaa
     1261 tgggagacgg ctattttgaa gttttccggt ttgactcata acggcagatt ctgcttgaca
     1321 gttaaagtaa aaaactttat ttttttaggt aaaattaatc tctctactta aatgctcgcc
     1381 acgattttaa aaaaatacgt atgggtaatt aacttatgcc ttctcacggg gctcgcttac
     1441 ctggcggcag ccaccgtaaa ccagaagata gaaggcatga ttagttcccc gcgtgcggca
     1501 gcctcccaaa aattccttca ggatgagagg gataacaaaa ctgaatcgac aaccaggcat
     1561 gccgtatcat actaccgcgt cattttaacg agaaacattt tcggcattac gaatctatcc
     1621 gattccaatt tgtcatcgac aaatcaggaa gccgcacccg ataccactct aaacatcgtc
     1681 cttctgggaa ccatcatcaa caaagacggc tcatccgtag caataatcaa aaatccggac
     1741 aataacaagg tcaacggata ccatagcgga gatactgtag acattatcag ttccgatgac
     1801 gtgaagcttg tcgaaatcac gaactgcaaa gtcgttatag acagacaaaa gaccggcaga
     1861 gaaacgatta aatgcaagga cctgggagac gtagcctcga ccgcccccat agcgccttcc
     1921 ggcaagagga tgctgaaaag aggagaggga tcggagagcc aggagaaatc aggtgaggac
     1981 gaggggatta agaaagtcgg ggaaaatgaa tacgaggtca gccgggagct attggaagaa
     2041 gtgctcagcg atcccacaaa catagtacag caggcgcggg tgattcctca ggaagacgga
     2101 ctcagatttt tcggtatcag atccaacagc atattctgga agattggaat aaagaacggc
     2161 gacacgctcc ataaaataaa taacgtcgag ctgaacgatg tggagaaagc cctgagtgta
     2221 ttcgaagagc tcaggtcaca aagcaatttc acgattgatt ttactagggc tggtaaagag
     2281 tacacatatg agtacaatgt tagatagaag tgcttacgga tgctcggttt atttgccgat
     2341 gtccgaatat ggaccttata cgatccgaac actcgggcag agataggata agcggacgca
     2401 gattgtgagg aatgattaat gagagcattg ctgataaata tcctgatact cggattcctg
     2461 ttaacgctat atgtgtacgt aaacacagac cacgaaaaaa catcgataaa agaaaacgcg
     2521 gcgcagacga ccgagctcga agataacggc tcaacactcc ctgtcggaaa tgagggttcg
     2581 agctacagct accgggtaga ggaaggagac actctgtccc ggatcgccga aaacgttcac
     2641 ctctccgtta aagaactgaa agaattcaac ggcctgacaa aggacgagct gtcggtcgga
     2701 gagtatatag acatcccggg cggaaaagat aataacggcg ttgagatcgc ggccaaagac
     2761 gattccaccc ccttcggtga tccggataat tcccaaacca tgacccagtc tctgagcgac
     2821 caggagcagt cccccgcgta taacgatcag ggctcttcgg accagcag
//
LOCUS       DASSJH010000297         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_931816, whole genome shotgun sequence.
ACCESSION   DASSJH010000297 DASSJH010000000
VERSION     DASSJH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931816"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFJ67_06395"
     CDS             <1..394
                     /locus_tag="VFJ67_06395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676375.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7290053.1"
                     /translation="MKKVAGTLRLELAQYREVEAFSQFASDLDKATQAQLARGSRLVE
                     ALKQGQYEPVAVEKQILIIFAVSNGYLDDYPVESVRKYEKEMNTFLESKYPQLLEEIR
                     SKKELTDEIRGKIVSALDELKKQLGDFN"
     gene            432..1316
                     /gene="atpG"
                     /locus_tag="VFJ67_06400"
     CDS             432..1316
                     /gene="atpG"
                     /locus_tag="VFJ67_06400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET7290054.1"
                     /translation="MASLRQIRTKIKSVKGTRRIMSAMKLISAVKLRRAQELLLSFRP
                     YSDAYLEITKSLARRCDPEKHPLLRKPESMKRLHLVFMTSDRGLCGSFNSNLIRKLET
                     YIVTDGQRYEDIKYTFIGRRGRDHFQRSKITGVQNFTGINERNFSEIAEKVAAELVSE
                     YVKDETDEIILVYNYFRSALTQVMVYERILPIEPLAESGEESAPIDYLYEPTREAVLK
                     KLLTNYVRVRVERAIEESLTSEHASRMTAMENATGNADDVIKKLTLLANKTRQSMITT
                     ELMDIVNGTEALRKGGAD"
     gene            1318..>2235
                     /gene="atpD"
                     /locus_tag="VFJ67_06405"
     CDS             1318..>2235
                     /gene="atpD"
                     /locus_tag="VFJ67_06405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7290055.1"
                     /translation="MESSNGNSMGKVIQVMGPVIDVEFKEGGLPPIYTALKLTNPLLN
                     DQKWNLVVEVAQQLGGNRVRCIAMDSTEGIRRGEDAMNTGQGITVPVGEQALGRILNV
                     VGEPIDEMGPVETGMRWPIHREAPEFVEQSTKLELFETGIKVIDLIAPFLRGGKIGLF
                     GGAGVGKTVLLMELIHNVAKEYGGVSVFGGVGERTREGNDFWIEMKESGVLANTGLVF
                     GQMNEPPGARARVALTALTLAEYFRDEKGQDVLLFIDNIFRFTQAGSEVSALLGRIPS
                     AVGYQPTLATDLGELQERITSTVKGSITSV"
BASE COUNT          617 a          551 c          656 g          411 t
ORIGIN      
        1 gatgaaaaag gtcgcaggaa cgctcagact cgagctcgcg cagtacaggg aagtagaggc
       61 attttcgcag ttcgcctccg acctcgacaa ggcgacacag gcgcagctgg cacgcggcag
      121 caggcttgtc gaggcgctca agcagggaca gtacgagcct gtcgcggtag agaagcagat
      181 attgattata ttcgcggtca gcaacggata tctcgacgac tatcccgtgg aatctgtcag
      241 aaaatacgag aaggagatga acacgttcct cgaatcgaag tatccgcagc tgctagagga
      301 aattaggagt aagaaggagc ttacggatga gatacgggga aagatcgtaa gcgcgctcga
      361 cgagctcaaa aagcagctcg gggatttcaa ttagatttca aaccaaatat tgaaccagag
      421 actaaaacga catggcaagc ttaagacaga taaggaccaa gattaaaagc gtgaaaggca
      481 cgcggaggat aatgagcgcg atgaagctca tatcagccgt aaaactgaga cgcgcccagg
      541 agctcctttt atcatttagg ccgtattcgg acgcttattt agagataacg aagagcctag
      601 cccgcaggtg cgatcccgaa aagcatcccc tcctgaggaa acccgaatcc atgaagcgtc
      661 ttcacctcgt tttcatgact tccgaccgcg ggctctgcgg cagcttcaac agcaacctca
      721 tcaggaaact cgaaacctat atcgtcaccg acgggcagag gtacgaggac atcaagtaca
      781 ccttcatcgg gagaaggggc agggatcatt ttcagaggag caagatcacg ggtgtacaga
      841 attttacggg catcaacgaa aggaatttca gcgagattgc cgaaaaggtg gccgccgagc
      901 tcgtaagcga atacgttaaa gacgagacgg acgaaataat actcgtttac aactacttca
      961 ggtcggccct cacgcaggta atggtatacg aaaggattct cccgatagag ccgcttgcgg
     1021 agtcgggaga ggaatcggcg ccgatcgatt acctctacga gcccacaaga gaagcggttt
     1081 tgaaaaagct gctcacgaac tatgtcaggg tccgcgtgga gcgggcgata gaggaatctc
     1141 tcacgagcga gcacgcgtcc aggatgacgg ccatggagaa cgcgaccggc aacgccgacg
     1201 acgtaataaa gaaattaacg cttttggcaa acaaaaccag acagtcgatg attactacag
     1261 agcttatgga catcgtaaac ggtacagagg ctttaaggaa aggaggcgca gattaacatg
     1321 gaatcaagta acggcaacag catgggcaag gtgatacagg tcatggggcc tgtaatcgac
     1381 gtcgaattca aggaaggcgg actgcctcct atatacacgg cgctcaagct cacgaacccg
     1441 ctattaaacg atcagaaatg gaacctcgtc gtggaagtcg cgcagcagct gggcggcaac
     1501 cgcgttcgct gcatcgccat ggactcgacc gagggcataa ggagaggcga ggacgccatg
     1561 aataccggtc agggtattac agtccctgta ggtgagcagg cgctcggcag aattctcaac
     1621 gtcgtcggcg agccgataga cgagatgggc ccggtcgaaa cggggatgcg ctggcctata
     1681 caccgcgagg cgcccgagtt cgtcgagcag agcacgaagc tcgagctctt cgaaaccggc
     1741 atcaaggtca tcgatctcat cgcgcctttt ctcaggggcg gaaagatcgg tctcttcggc
     1801 ggcgccggag tcggcaagac ggttctgctc atggaattga tacataatgt tgcaaaggaa
     1861 tacggcggcg tctctgtgtt cggaggcgtc ggagaaagga cgcgagaagg aaacgacttc
     1921 tggatcgaga tgaaggagtc gggagtattg gccaacaccg gtctagtgtt tggtcagatg
     1981 aacgagcccc cgggcgcgag ggccagggtt gctcttacgg cgctcacgct cgccgagtat
     2041 ttccgggatg aaaaaggcca ggacgtacta ctatttatcg acaacatatt caggttcact
     2101 caggcgggct ccgaggtctc ggcgctcctc ggtcgaatcc cttcggccgt aggttatcag
     2161 ccgacactcg cgaccgacct tggagagctc caggagagga ttacatcgac ggtcaaaggc
     2221 tcgatcacat cggtc
//
LOCUS       DASSJH010000298         5474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_505788, whole genome shotgun sequence.
ACCESSION   DASSJH010000298 DASSJH010000000
VERSION     DASSJH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505788"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFJ67_06410"
     CDS             <1..580
                     /locus_tag="VFJ67_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290056.1"
                     /translation="SNFTLSRFIAVSRDSDVLFNVTLVASPGEIIVNDWTVFQNKIRE
                     DGTDEFNFDSLEADFEIDGDGDTCIRAHGESLVQITARSITLADCNIGIDTQSSGFVM
                     LESDLDITISANKDAIKTSNESFVRVTQTVTPVDNNIFITSLKEHGIRAAGSSEVEIN
                     PQNDCSISGAKSAIDQKDSSVVDPDGCTLIDG"
     gene            complement(639..3344)
                     /gene="polA"
                     /locus_tag="VFJ67_06415"
     CDS             complement(639..3344)
                     /gene="polA"
                     /locus_tag="VFJ67_06415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET7290057.1"
                     /translation="MEKPVLYLIDGSSYIFRAYYAIRRLSTSKGFPTNAVYGFASMIF
                     KFLKDYKPGYLGIVFDTKEKTFRDEIYPLYKANRGAPPDDLVPQFDKIFEVVDAFRIP
                     QFRVDGFEADDVMGTIARDYERRGMDVVLVTGDKDFCQLVSDKVTLLDTMKNKETRTE
                     DVIEKYGVPPEKVTDVFALMGDQVDNIPGIKGIGEKTAIGLIKTFGSLDNLLRNTDDL
                     AEKQRKLITERKDDALLSKELVTIKTDMDLDMELEKFKYDGFDKAKLRGIFEELEFKN
                     LLKELGDGGVVEKKTDEGGGAVPYDGYSLVLTEDALERVIERIKKAGEVSIDLETTST
                     DPMLAEIVGIALCPAPHEAYYVPVAHRALSDSSTRQLALPYVLGKLKPLIEDEGIRKI
                     GQNIKYEFVLLEMYGLRLGGISFDTMIAAHMLDSSRMSYSLDELCRLYLGHHMISYRD
                     VTGTGKSRINFEEVELEAAKVYSCEDADVAMLLSEKLAPELEAAGLTRVFRDIELKFI
                     EVLARIEINGVKVDAVRLKELSKEFDSLLKDIEKAIYEEVGYEFNLNSPIQLRQVLFD
                     TLGLPQKKLTKTGESSTDVEVLNDLSKFHPVPEKVLEHRTLSKLKSTYVDALPRLINP
                     KTGRLHTSFNPVGSSTGRLSSSEPNLQNIPIKTAQGRRIREAFIPEEGCTMLSADYSQ
                     IELRLLAHFSGDESLIEAFMTGSDIHNRTASEIFGVTPDLVTPEMRRLAKSINFGIIY
                     GISAFGLAKQLGTSVSIAKSYIDEYFKRYKKVREYIEKTINDARSKGYAETILGRRRP
                     IPELASEDRNRKGFGERTAMNTPIQGSAADIINIAMIRIHEKLAAGHKSRMILQVHDE
                     LLFEVPGDELDYVSRMVREEMEGAWKLSVPILVEIGTGSNWAEAH"
     gene            3500..3844
                     /locus_tag="VFJ67_06420"
     CDS             3500..3844
                     /locus_tag="VFJ67_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET7290058.1"
                     /translation="MKYLLIQWIISTASLLIVSYIIPGFVVEGIGNALLAAVIIGLIN
                     ATIGLFLKIITLPISILTFGIFLLVINALMLMLASSIAPGFHVSSFWTALLGALVLAI
                     VSSILRHLLGEK"
     gene            complement(3849..4874)
                     /locus_tag="VFJ67_06425"
     CDS             complement(3849..4874)
                     /locus_tag="VFJ67_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7290059.1"
                     /translation="MRVAMYYNNRDIRLEEMPTPAIGPGEILMRVRASGICGSDLMEW
                     YRLPKAPLVLGHEVSGEVVETGEGVDKFRPGDRVIATHHVPCNTCYHCLRGNHSACRT
                     LRTTHFDPGGFSEYIRIPAINVDRGVLRLPDEVSHDEASFVEPLGCVVRGHTLAGFRV
                     SDSVLVMGSGITGLLHIQLARALGAGRIIATDISGFRLGAASRFGADHTVGAGEEVAS
                     YLRNVNEGRLADIVIVCTGARQAIEDSFSLVDPGGTILFFAPSSPEAELRIPYNEFWW
                     SGIKTVSSYAASPSDLATALELIRAKRVNVAGMVTHRLALKETREGFRMVEEAGDSIK
                     VIIDPGL"
     gene            complement(4926..>5474)
                     /locus_tag="VFJ67_06430"
     CDS             complement(4926..>5474)
                     /locus_tag="VFJ67_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965428.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HET7290060.1"
                     /translation="EGLTTSFRDAIRLNAAGVGLSVFIGSEHEKQTLLNLAKLVDKGE
                     DYGIPVLGITAVGKELEKRDARYLALSCRICAELGTHIVKTYYCEDFEKVTASCPVPI
                     VIAGGPKLETEMDVFEMTYNAIRQGAVGVDMGRNIWQNDHPVAVIRAIRSIVHENGNP
                     KQAHDLFLTLKNEETKKVARVK"
BASE COUNT         1096 a         1557 c         1521 g         1300 t
ORIGIN      
        1 atccaatttc actctctcta ggttcatcgc ggtttcgcgc gacagcgacg tattattcaa
       61 cgtgacactc gtagcatcgc ccggcgagat aatagtaaac gactggacgg tgttccagaa
      121 caagatcaga gaggacggca cggacgaatt taacttcgac tcgctcgaag cggatttcga
      181 gatagacggt gacggagaca cctgtataag ggctcacggg gaaagcctcg tgcagataac
      241 ggccaggagc atcactctcg ccgattgcaa tatcggcata gacacgcaga gctcggggtt
      301 cgttatgctg gaatccgatc tggatataac cataagcgcc aataaggacg ctattaagac
      361 cagtaacgaa tcattcgtca gggtcacgca gacagtcacg cccgtcgaca acaatatatt
      421 tataacctct ctaaaagaac acgggatcag ggcggcaggc tcctcggagg tcgagatcaa
      481 tcctcagaac gactgttcca taagcggcgc gaagtcggcg atagaccaga aagactcgtc
      541 cgtggtcgac ccggacggat gcacgctgat agacggctag acgggctcaa tacgcagcaa
      601 agctaagaca gagaattcct gataagcggc gggcgggatc agtgggcttc cgcccagttg
      661 ctcccggtgc ctatttcgac caggatcggt acgctcagct tccatgcccc ttccatctct
      721 tccctcacca tgcgggatac gtaatcgagc tcgtccccgg ggacttcgaa gagaagctcg
      781 tcgtgtacct gcagaatcat gcgcgacttg tgaccggccg cgagcttctc gtgtatcctg
      841 atcatggcga tgtttattat gtcggcggcc gagccctgaa taggcgtatt catggccgta
      901 cgctcgccga aacccttcct gttcctgtcc tcggaggcaa gctccggtat gggacgtctc
      961 ctgccgagaa tggtctccgc gtatcccttg ctccgggcgt cgttgattgt tttctctata
     1021 tattccctga ccttcttata gcgcttgaag tactcgtcta tatagctctt ggcgatagag
     1081 acggaagtgc cgagctgttt cgccaggccg aaggcgctta tgccgtaaat gatgccgaaa
     1141 ttaatgctct tagcgagcct cctcatctcc ggggtcacga ggtccggcgt cactccgaat
     1201 atttctgatg ccgtcctgtt gtggatgtcg ctccctgtca tgaaagcctc gatgaggctc
     1261 tcgtcgccgg agaaatgggc gaggagcctc agctcgatct gggagtagtc ggcagagagc
     1321 attgtacaac cctcttccgg aatgaacgcc tcccttatcc gtctcccctg ggcggtcttt
     1381 atcggaatgt tctggagatt gggctcgctc gagctgagcc tccctgtgga tgagcccacg
     1441 ggattgaacg atgtgtggag cctgccggtc ttcgggttga taagcctcgg aagggcgtcc
     1501 acgtaagtgg acttgagctt ggatagcgtc ctatgctcga gcaccttctc ggggacggga
     1561 tggaacttgc tgaggtcgtt aagcacctcg acgtcggtcg aggactcgcc cgtcttcgtg
     1621 agcttcttct gaggaaggcc gagggtatcg aacagcacct gtctaagctg tatgggcgag
     1681 ttcaggttga actcgtaccc tacctcttcg tatatggcct tttctatatc cttgagcaat
     1741 gagtcgaact ctttcgagag ctctttgagc ctgactgcgt ccacctttac gccgtttatc
     1801 tcgatgcgtg cgaggacctc tataaacttc agctcgatat ccctgaaaac acgcgtgaga
     1861 ccggctgctt ccaactcggg cgcgagcttc tccgaaagga gcatggccac gtccgcgtcc
     1921 tcgcaggaat agaccttggc cgcttcgagc tccacttcct cgaagtttat tcgtgatttt
     1981 cccgttccgg tcacgtccct gtaggagatc atatggtgtc ccagatagag gcggcagagc
     2041 tcgtccaggc tgtagctcat cctcgaggag tcgagcatgt gcgcggcgat catcgtatcg
     2101 aatgagatgc cgcccagcct gagcccgtac atctcgagca acacgaactc gtatttgatg
     2161 ttctgtccga tcttccggat gccctcgtcc tcgatgaggg gtttcagctt gccgagcaca
     2221 tacgggagcg cgagctggcg ggtcgaggaa tcggagaggg ccctgtgcgc tacagggacg
     2281 taatatgcct cgtgaggggc ggggcagagc gctatgccga ctatctcggc gagcatcggg
     2341 tcggtcgaag tggtttcgag gtcgatcgag acttcgcccg ctttcttaat cctctctata
     2401 acgcgctcca gcgcgtcctc cgtgagcacg agagagtagc cgtcgtaggg gacagccccg
     2461 ccgccttcat ccgttttctt ctcgacgacg cccccgtcac ccaactcttt caggaggttt
     2521 ttgaactcga gctcttcgaa tatgccccta agcttcgctt tgtcgaaccc atcgtatttg
     2581 aatttttcga gctccatgtc gaggtccata tccgtcttta tcgttacgag ctccttgctg
     2641 agaagagcgt cgtccttcct ctccgtaatg agcttccgct gcttttcagc gaggtcgtcc
     2701 gtattccgca gcaggttgtc gagagagccg aacgtcttga taagccctat ggccgtcttc
     2761 tctcctatgc cctttatacc ggggatgttg tccacctgat cccccatgag cgcgaaaacg
     2821 tcggtcacct tttcgggagg cactccgtat ttctcgatga cgtcctccgt gcgggtttcc
     2881 ttgttcttca ttgtatcgag gagcgtgacc ttgtcggaga cgagctggca gaagtcctta
     2941 tctcccgtga cgagaacgac gtccatcccg cggcgctcgt agtccctggc tatagtgccc
     3001 attacgtcgt ccgcctcgaa cccatccacg cggaactgcg gtatcctgaa cgcgtcaact
     3061 acttcgaata tcttgtcgaa ctgcgggacg aggtcgtccg gcggggcgcc cctgttggcc
     3121 ttatagagcg gatatatttc gtccctgaac gtcttttctt tcgtatcgaa aacgatgccc
     3181 agatagccgg gcttatagtc cttcaggaac ttgaaaatca tcgaggcgaa cccgtagacg
     3241 gcgttagtcg ggaacccttt cgaggtgctg agacgcctta tcgcgtaata cgcgcggaaa
     3301 atgtaggagc ttccgtcgat gagatataaa acgggttttt ccataagcgt acagtttagg
     3361 ttttaaaggg aggagtgtca aaaaccggag ctaagcgccc ctgtcgcacg cgccgctttc
     3421 tatttgaaat aaaatgtgaa taatatttga gatattcgga gaaccgctct atgggcataa
     3481 aagctaaagg ggggatatca tgaaatacct tttgatacag tggataatca gcaccgcaag
     3541 tctgcttatc gtgtcttata taattcctgg gttcgtcgtg gaggggatag gcaacgcgct
     3601 gctcgccgcc gttataatag gtctgatcaa cgcgacgata ggactcttcc tcaagataat
     3661 cacgctcccg atctcgattc ttactttcgg aatattcctt ctggtgataa acgcgctcat
     3721 gctcatgctc gcctcgtcga tagcgcccgg attccacgtg agcagtttct ggacagcgct
     3781 cctcggagcc ctggtgcttg ccatcgtgag ctcgatcctg cgccatctgc tgggagaaaa
     3841 ataggtgttc aaagaccggg atcgataatg accttgatcg aatccccggc ctcttcgacc
     3901 atccggaaac cctcgcgggt ttccttgagt gcgagcctgt gagtgaccat tccggccacg
     3961 ttcacccttt ttgcccttat cagctcaagt gctgttgcca ggtccgaagg ggatgcggcg
     4021 tatgagctga ccgtctttat ccccgaccac cagaattcgt tgtagggaat ccttagctcc
     4081 gcttcaggag aggaaggagc gaaaaagagt atggtccctc ccggatcgac gagactgaaa
     4141 gagtcctcta tagcctgccg cgcgcccgtg catacgataa caatgtccgc gaggcggccc
     4201 tcgttgacgt tgcggaggta agaagcgact tcctcaccag ccccgaccgt gtggtcggcg
     4261 ccgaaacggg acgccgcacc gagcctgaag ccgctgatgt cggtcgcgat tatccgtccc
     4321 gccccgagtg cgcgggcgag ctgtatgtgc aatagtcccg ttatgccgct ccccatgacg
     4381 agcacgctgt cggatacgcg gaagcccgcg agcgtatggc cgcggactac gcatccgagg
     4441 ggctcgacga acgacgcctc gtcgtgggag acctcgtccg ggagcctaag cacccccctg
     4501 tccacgttta tcgcgggtat gcgtatgtat tccgagaaac cgccgggatc gaaatgggtc
     4561 gtcctcagcg tcctgcacgc cgagtgattg ccccttaaac agtgatagca cgtattgcag
     4621 gggacgtggt gcgtggcgat gacgcggtcg cccggcctga atttatcgac gccttcgcct
     4681 gtttcgacca cctctccaga gacctcgtgc cctaggacga gaggggcctt cgggaggcgg
     4741 taccattcca tgaggtcgct cccgcatatt ccgctcgcgc ggacgcgcat gagtatctcg
     4801 cccgggccga tcgcgggcgt gggcatctcc tcgagcctga tatccctgtt gttatagtac
     4861 atcgcgacac gcatgtcttc tccgtgtgtt tgcggcaacg gctccggccg gtttacgaag
     4921 ccggttcatt ttacgcgggc tacttttttc gtttcctcgt ttttaagcgt gaggaagagg
     4981 tcgtgagcct gcttgggatt gccgttctca tggacgatcg accttatagc cctgatcacg
     5041 gcgacagggt gatcgttctg ccagatgttc ctgcccatat cgacgccgac ggccccctga
     5101 cgtatcgcgt tgtaggtcat ctcgaagacg tccatctcgg tctcgagctt gggcccgccg
     5161 gcgatcacta tcggcacggg acagctcgca gtaaccttct cgaagtcctc gcagtagtag
     5221 gtcttgacga tatgggtgcc gagctcggcg catatgcggc aggagagcgc aagatacctg
     5281 gcgtccctct tctctagctc tttcccgaca gccgtgatgc ccaggacagg gatgccgtag
     5341 tcctcgccct tatcgacgag cttcgcgaga tttaggaggg tctgcttctc gtgctcgctc
     5401 ccgatgaaaa ctgatagtcc cacaccggcg gcgttcagcc ttatggcgtc cctgaacgag
     5461 gtcgtgaggc cctc
//
LOCUS       DASSJH010000299         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1371251, whole genome shotgun sequence.
ACCESSION   DASSJH010000299 DASSJH010000000
VERSION     DASSJH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371251"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..890)
                     /locus_tag="VFJ67_06435"
     CDS             complement(66..890)
                     /locus_tag="VFJ67_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7290061.1"
                     /translation="MKAEHFKLLGREIFIRYRIGGGNNTPVFFIHGIGESGWCFFDAF
                     GLTKKYDIVVPDLLGFGKSEKVKDSPDYSFSSQVKILREIMNLFDLRDIALVGHSYGG
                     VLGTFLCGDDDKGRIKKFVNIEGCITRDTLVLSTSAANAFEDCKGDVSEFGKWLREGG
                     FRKSVLEDNESASTIKYFDSVTECDPEAFAQTAAEFIKRTEGEDEQGNNELSLAYKGL
                     RLPRIYCVGARPVMNSTKSFLSRNNLVWKGFDVVSHWVMLDAKEEFYAFLEGYIES"
     gene            1090..1872
                     /locus_tag="VFJ67_06440"
     CDS             1090..1872
                     /locus_tag="VFJ67_06440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7290062.1"
                     /translation="MRLKDKVAVITGGSSGIGRAITLGYIGEGAKVVIAALHEDPCLE
                     VVREVEALGGRAMYYTLDVSDLSRHQELMDKAVSTFGKVDILVNDAGYARRETVFDVT
                     PEGWDKMMDINLKSAFFLSQCFARQFIKQGTPGRIINIGSVAGAMDMHPISIAYHAAK
                     AAMINMTRVMAVDLAHYNITVNCIGPGSTITPLSASRNPEYDDYMTRGIPEGRRGTPE
                     DMVPPAVMFATDEASYITGQTIFVEGGAMSVYLGKDEPDFKA"
     gene            complement(1883..>2341)
                     /locus_tag="VFJ67_06445"
     CDS             complement(1883..>2341)
                     /locus_tag="VFJ67_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290063.1"
                     /translation="AFLITMDFFVSIPRKDWDRKVEEGNLRLRDWYSIDKALEILLAI
                     KSDKSLVYGDLYDVETGTMRGCITIDPGRYKYFILEGLFSLDDELDGLIDLGIFVDTP
                     PGVALARAETRDESKRHLDPHGWIEKKEIYFDGYLPYIDAHRKKADVVLG"
BASE COUNT          584 a          628 c          566 g          563 t
ORIGIN      
        1 aataagagcc ttcaacctac tcgcggctct ctaacactat gctatcacta cacaattatc
       61 gctcatcagg attcgatata accttccagg aaggcgtaaa attcctcttt cgcgtcgagc
      121 attacccaat gagagacgac gtcaaaccct ttccatacaa gattatttct ggacaaaaaa
      181 ctcttggtcg aattcatgac cggcctcgcc cccacacagt agattctggg caatcggagt
      241 cctttatacg cgagtgaaag ctcattattg ccctgctcgt cctcgccctc cgtcctcttt
      301 ataaattcag ctgccgtttg ggcaaatgcc tcggggtcgc actcggtaac ggaatcgaaa
      361 tactttatgg tcgaagcgct ctcgttatcc tcgaggacac tcttcctgaa acctccttca
      421 cggagccatt taccgaattc cgacacatcg cccttacagt cctcaaacgc attcgcggca
      481 ctcgtcgata agaccagtgt gtcccgtgtt atgcaccctt cgatgttcac gaattttttg
      541 atcctgcctt tatcatcgtc cccgcacaag aacgtcccga gcacaccgcc gtaagaatgg
      601 ccgaccaggg ctatatccct gagatcgaag aggttcatga tctccctgag aattttcacc
      661 tgggacgaga atgaataatc gggggagtcc ttaacttttt cacttttacc gaaaccgaga
      721 aggtcgggaa ctacaatatc gtatttcttc gtcagaccga aagcgtcaaa gaaacaccag
      781 ccggactcac ctatgccatg aatgaagaat accggagtgt tattgccccc gcctatcctg
      841 tatctgataa aaatttcgcg gccaagcagc ttgaaatgtt cagccttcat cgcttatcct
      901 cctgccggat attataaagt acatactcat cttattccct cattatcaat acggtcttcc
      961 tttttataga caattgtagt atcaatttat tagctttgtc ccttggattt tatctagaca
     1021 gggtataatc cggatcgatt gtaaaattac cggcattacc tgaacaaatc accggaagag
     1081 ggtatcagga tgagattaaa agataaagtc gcggttatca cggggggctc gtcgggcatc
     1141 gggcgggcga taactctcgg atacatcggg gaaggcgcca aggtcgtgat cgccgctctc
     1201 cacgaggacc cgtgcctgga ggtggtaagg gaagtggaag cccttggcgg ccgggccatg
     1261 tattacacgc tcgacgtttc agacctgagc aggcaccagg agctgatgga caaagccgta
     1321 agcacgttcg gcaaggtgga catactcgtg aacgacgccg ggtatgccag gcgcgagacg
     1381 gtattcgacg tgacgcctga gggctgggac aagatgatgg atattaattt gaaaagcgct
     1441 tttttcctgt cccagtgctt cgccagacaa ttcatcaagc agggtactcc cggcaggata
     1501 ataaacatag gctccgttgc gggcgcgatg gacatgcatc cgatatctat cgcctaccac
     1561 gcggcgaaag cggcaatgat aaacatgacg agggtgatgg cggtcgacct cgcccactac
     1621 aacatcacgg tcaactgcat aggcccgggc tcgacgatca cgcctctctc ggcgtccaga
     1681 aatcccgaat acgacgacta tatgacgaga ggtattccgg agggacggag gggcacacct
     1741 gaagatatgg tgcctccagc agttatgttc gcgacggacg aggcctctta catcacaggc
     1801 cagaccatat tcgtcgaggg cggggcgatg tcggtctatc tagggaagga cgagccggat
     1861 ttcaaggcgt gatggtatcg cctcagccga ggaccacatc ggccttcttt ctgtgcgcgt
     1921 ctatgtaggg aagatagccg tcgaagtaaa tttccttttt ctcaatccat ccgtgagggt
     1981 cgaggtgccg cttcgattcg tctctcgttt cggctctcgc gagagcgacg ccaggcggcg
     2041 tatccacgaa aatccccaaa tcaataagcc cgtcgagctc gtcgtccagg gaaaagagcc
     2101 cttcgagaat gaaatactta tatctgccgg gatcaatcgt gatacatccc cgcatggtcc
     2161 cggtttcgac atcgtacaag tccccgtata cgagggattt atcagatttt atagcgagaa
     2221 gaatttccag cgccttatct atgctgtacc agtcccggag gcgtaaattc ccctcttcta
     2281 ccttcctgtc ccaatctttc ctgggtatcg agacaaaaaa atccatcgtg atcaggaatg
     2341 c
//
LOCUS       DASSJH010000300         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1480073, whole genome shotgun sequence.
ACCESSION   DASSJH010000300 DASSJH010000000
VERSION     DASSJH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480073"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /gene="rodA"
                     /locus_tag="VFJ67_06450"
     CDS             <1..904
                     /gene="rodA"
                     /locus_tag="VFJ67_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET7290064.1"
                     /translation="IYGFSLLLLLFVLVFGKEVSGSRSWITIGSFATLQPSELAKIPI
                     ILAIARFYDNDYEGVPYGLLDLVKPLLLIALPLSLVMLQPDLGTSLTIILISGSMILF
                     MGVEKKLIFIIIAIVVGFSYPAWHFFLKPYQKDRIKTFIEPSKDPLDSGYNAIQSQIA
                     VGSGRFTGKGFMSGSQTQLRFIPAQQTDFAFSVLAEEWGFLGGFMALLLYFMIILWIL
                     DTASRAKDKFSVLVCFGIAAMYFWHVVVNVGMVIGLLPIAGVPLMLLSYGGSSTLTAL
                     IGIGIVLGIRMRKFPIQGPAVELR"
     gene            complement(897..1874)
                     /locus_tag="VFJ67_06455"
     CDS             complement(897..1874)
                     /locus_tag="VFJ67_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET7290065.1"
                     /translation="MVSITDIEGIKVGHASDFDAVTGCTVILFDNPATGAIDLRGGGT
                     STRQIDSLLSHNTFGKVHAILLTGGSAYGLDASGGVMKYLEERNKGLSVGYGMVVPSV
                     PTAVIFDLGIGNGSVRPDARMGYESCLNADSSPVEEGSVGAGTGATVGKLLGLDHATK
                     GGIGAAGYKFENGIIVAVLVVVNAFGDIISPENGEIIAGVRTTPRGRKFAGTVKLFKE
                     GVKFRDVKYQNTTLTVVATNARFTRSELGRIANIAQTGVARVVSPVHTVADGDVVIAV
                     SCGETDGDANLVGIIAAELMGTAILRALHHSKGLGGIPSASDLKGEEIT"
BASE COUNT          430 a          638 c          561 g          578 t
ORIGIN      
        1 catctacggc ttctcgctcc tcctcctcct gttcgttctc gttttcggca aagaggtctc
       61 cggttccagg agctggatta cgatcggctc tttcgccacg cttcagcctt cggagctggc
      121 gaagataccg attatcctcg ccatagccag gttctacgac aacgattacg aaggggttcc
      181 ttacgggctg ctcgacctcg taaagccgct gcttctgatc gcgcttcctc tctcactcgt
      241 catgctccag cccgacctcg ggacctcgct tacgatcatc ctcatatcgg ggagcatgat
      301 cctgttcatg ggggtcgaga agaagctgat atttattatt atcgcgatcg tcgtggggtt
      361 ctcttaccct gcatggcatt tctttctcaa gccgtatcag aaggacagaa taaagacgtt
      421 catcgagcct tccaaagacc cgctcgattc cggatacaac gccattcagt ctcagatagc
      481 ggtaggctcg ggcaggttca cgggcaaggg gttcatgtcc ggctcccaga cacagctcag
      541 gttcatcccg gcccagcaga ccgacttcgc cttctcagtg ctcgccgagg aatggggttt
      601 cctagggggc ttcatggcgc tccttctata ctttatgata atcctctgga tacttgacac
      661 ggccagccgc gcgaaggaca agttttcggt tctcgtctgc ttcggaatcg cagcgatgta
      721 tttctggcac gtggtcgtga acgtcggaat ggtcatcggt ctcctcccga tagccggggt
      781 gccgctgatg cttttgagct acggcgggtc gtccactctc acggccctga tcggcatagg
      841 cattgtgctc ggcataagga tgaggaaatt ccccatccag ggccctgccg tcgagctcag
      901 gtgatctcct ctcccttgag gtcgctcgcg ctcggtatcc ctcccagccc tttcgaatga
      961 tgaagcgcgc gcaggatggc ggtccccatc agttctgctg caatgattcc tacgaggttc
     1021 gcgtccccat ccgtctctcc gcacgagacc gcgataacta cgtcgccgtc ggcgaccgtg
     1081 tgcacgggcg atacgaccct agcaacccct gtctgggcga tattggctat cctgccgagc
     1141 tcggatctgg tgaatcttgc gttagtcgcc acgaccgtga gcgtagtgtt ctggtatttg
     1201 acgtccctga acttaacccc ttctttgaag agttttacgg ttcccgcgaa ttttctcccg
     1261 cgcggggtgg tcctgacgcc ggcgattatc tctccgttct cgggcgagat tatgtctccg
     1321 aaggcgttta cgacgaccag caccgccact atgattccgt tctcgaattt gtagcccgcc
     1381 gctccgatcc cccccttggt cgcgtggtcg agtccgagta gctttccgac tgtagcccct
     1441 gttcctgccc ctacgctccc ctcttcgacc ggggaggaat cggcgttcag acacgattcg
     1501 tagcccatac gggcatcagg cctcacgctg ccgtttccga tgccgagatc gaagatcacg
     1561 gccgtcggaa cggacggaac taccataccg tacccaacgc ttagcccctt attcctttct
     1621 tcgagatact tcatcacgcc gcccgacgcg tctagaccgt aagcgctccc gcctgtgagc
     1681 aggattgcgt gtaccttgcc gaatgtatta tgggagagga gcgagtctat ctgcctcgtg
     1741 ctcgtgccgc ccccccttaa atcgatcgca cctgtcgcgg ggttgtcgaa gagtatcacc
     1801 gtgcatccgg tcacggcgtc gaaatcggaa gcgtgaccaa cctttatgcc ttcaatatcc
     1861 gttatagaga ccatgacgtt tattctaaaa tcaatatttt ttatttcaaa gtttgtgcct
     1921 gttagctgcc ctttccggcc acttgacatg aaaggataat aggatagaat cttccacgat
     1981 ttaaggttat tagggttcgt gagtatggga atccgtaacc tgtttttatt ttaataaaat
     2041 atcctgtgtc caccccaaat tatgataaga aatcgtgttt ccgggaatga acggtgttgt
     2101 gtgacgggaa atattatgaa ggcgtgacgc tggatagact tcaaaatatg tataatccga
     2161 gagtgctatt gacggtaacg gttctttatt tgcttctttc aacgagc
//
LOCUS       DASSJH010000301         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_506472, whole genome shotgun sequence.
ACCESSION   DASSJH010000301 DASSJH010000000
VERSION     DASSJH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506472"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFJ67_06460"
     CDS             complement(<1..579)
                     /locus_tag="VFJ67_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290066.1"
                     /translation="MKRLFLIVIVNIAVFCTLIVLAEVAGQTYYYVTHGRFIFQNPVS
                     AYHFEGDDPNQYQYHSMFEMHPLLAVRPRKDVRIKNYINDKVITTTDRYTRWTGAPED
                     DSKLIRVAISGGSTAFGTGVTDSDSWPALLQAKLGDRFSVINYGVPCYSTAEAMIQMA
                     LTIPEVKPDFIILYHGWNDIILYHHKDINPDYF"
     gene            complement(884..2350)
                     /locus_tag="VFJ67_06465"
     CDS             complement(884..2350)
                     /locus_tag="VFJ67_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7290067.1"
                     /translation="MAFPTKTIENFVKAGYPILYLVSWEEERVENTLKTIGKNIHGDS
                     ARFSVWSCTEGFREGAENLVKALDRVMTEQVKGFYVFKDVNYFLDDPRLVRKLKDAYQ
                     KLRKSSNTIIILSNTLSVPIELEKEVAVIDIDLPDRSETGKIFDFVIKSYTPESVQKS
                     MKPYFKEAAINALQGLGALEMELALRRTFVGRDELGEDAVDALLEEKAQLVRKSGTLD
                     FVRSRVDIDKVGGLENIKEWLEVRRLAFSSEAKEFGLDTPKGILLMGISGCGKSLCAK
                     AIATAWNLPLLRLDLNQVYSEAFGSPEEAFRKAIKTVEATAPCVLWIDEIEAGITRSG
                     EKSGDSPTSRIFGYFLTWMQEKAYPVFVTATANQIDLLPPEILRKGRFDEIFFITLPN
                     QKERKEIFRIHLQNRGKNPESFNLDSLAKNTEGLSGAEIEQAVISALFESYSKGKELD
                     DRELIISASSIVPLSTTMREEISKLERWASNRAVKASR"
     gene            complement(2423..>3931)
                     /locus_tag="VFJ67_06470"
     CDS             complement(2423..>3931)
                     /locus_tag="VFJ67_06470"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET7290068.1"
                     /translation="LRDGEQAPGCGMTAEEKLRVAHQLEKLGVDVIEAGFPISSEGDF
                     ESVKIIAEKIRGCEIAGLCRANFNDIDRGWEAVKGAEYPRIHTFIATSDIHLKHKLRK
                     SRDEVLEIISTAVKHAKHYTENVEFSCEDATRTELDYLCRAVDVAVRSGAKIINIPDT
                     VGYTVPEEFSYIIRTLKERVPGLDNIVLSVHCHNDLGLAVANSIAAIREGARQVECTI
                     NGLGERAGNASLEEIVMGLKVRNDRNPFTTRINTDQLYPTSRLVSQVTGVSVQPNKAI
                     VGANAFAHEAGIHQDGVLKERITYEIMNPEDVGIPSNKLILGKHSGRHAFKDRLEDYG
                     YFLDDKSFENAFRKFKDLADKKKYVFDEDIEAIISEEFVRSSDFYKLISANYSGGSDM
                     QPVATVKLLIDGKEVTISESGDGPVDAAYQAVSKATGLNPTLESYVVTAITEGIDAQG
                     EVTVRVEDEGVITQGQGSNTDIVVASVKAYVNALNKLRWRKEHPKRVTSQGM"
BASE COUNT          806 a         1129 c          932 g         1064 t
ORIGIN      
        1 aaaatagtcc gggtttatat ccttatgatg gtaaagaatt atatcgttcc agccgtggta
       61 gaggattata aagtccggtt tgacttcggg gatcgtcagc gccatttgta tcatcgcttc
      121 ggctgtggaa taacacggga ctccgtaatt tatgacgcta aaccggtccc cgagcttggc
      181 ttgcaaaagc gcaggccagc tgtcgctgtc agtcacgccg gtgccgaacg ccgtcgatcc
      241 gcccgaaata gccaccctga ttaatttgct gtcgtcttcg ggagcgcccg tccacctggt
      301 atatctatcg gtcgtagtta ttaccttgtc attaatgtaa ttcttgattc tcacgtcctt
      361 tctgggtctt acggccagta acggatgcat ctcaaacatc gaatggtatt ggtattgatt
      421 gggatcatct ccctcaaaat gatacgcgga aacaggattc tggaatatga acctaccgtg
      481 agttacgtag tagtatgttt gacctgctac ctccgcgaga acgatcaacg tgcaaaatac
      541 cgcgatattc acgataacga tcagaaacaa ccttttcatt agcccgctta ctcttattag
      601 ttttgaatct aaagacgctt tattaactgt attatgtgtt agctcgatac gcaaataatc
      661 cataaaacgt cattttgaat ttagagtctt ccccccaaga gcagggcatt accctaactg
      721 gcggctggcg cttattcagc tcgatcccgg cccgaatcca gtcaggtgcg ccgagtccct
      781 gtgccgccaa tgcagatcac ctccaggctg agctacattt caccgtcttc ggttgcgaga
      841 gtttatatga attttttgga actgaaaaga aatcccggaa aaatcaccgc gatgctttga
      901 ccgccctatt cgaagcccag cgctcgagtt tcgaaatctc ctccctcatc gtcgtcgaga
      961 gaggcacgat tgacgaggcg gagattatga gctccctgtc gtcgagttcc ttccctttgg
     1021 agtaagactc gaagagagca gatatgacgg cctgctctat ctcggccccg ctcagccctt
     1081 ccgtgttctt ggcgagtgaa tcgaggttga agctttccgg gttcttcccc ctgttttgga
     1141 gatgaatcct gaaaatctct ttcctctctt tctggttcgg gagcgtgatg aagaatatct
     1201 cgtcaaacct tcccttacgg agaatctctg gcggaagcag atctatctgg ttcgcggtcg
     1261 ccgtgacgaa aaccggatac gccttctcct gcatccacgt gaggaagtac ccgaatatcc
     1321 tcgacgtcgg gctgtccccc gatttctctc cgctcctcgt tatgccggcc tcgatctcgt
     1381 ctatccagag cacgcacggg gcggtcgcct caactgtctt tatcgccttt ctgaacgcct
     1441 cttcagggct cccgaacgcc tcgctataca cctggttcag atcgagccta agcagaggca
     1501 ggttccatgc ggtcgctatt gccttcgcgc agaggctctt cccgcacccg ctaattccca
     1561 tgagcagtat tcccttggga gtatcgagac cgaactcctt cgcctcgctc gaaaatgcga
     1621 gccttctcac ctcgagccac tctttaatgt tttcaagacc gcctacctta tctatgtcca
     1681 ccctggatct cacgaaatcg agcgtcccag atttcctcac gagctgcgcc ttctcctcga
     1741 gaagcgcatc aacggcatcc tccccgagct cgtcccttcc aacgaacgtc cttctcaggg
     1801 ctaattccat ctcgagcgcg ccgagaccct gaagcgcgtt tatagccgct tccttgaaat
     1861 acggtttcat agatttctga acgctctcag gcgtatatga tttaatcacg aagtcgaata
     1921 tcttgcccgt ctcggagcgg tcgggaagat cgatgtcgat tacggccacc tctttctcga
     1981 gctcgatcgg cacagaaagc gtgttcgaga gtatgattat cgtattggac gatttcctga
     2041 gcttctggta cgcgtccttt agtttcctga ccagcctcgg gtcgtcgagg aaataattca
     2101 cgtccttgaa tacgtaaaac cccttaacct gttccgtcat caccctgtcg agagctttta
     2161 cgaggttttc ggctccttcc ctgaatccct ctgtgcacga ccagacggaa aacctggccg
     2221 aatccccgtg gatattcttc cctatcgtct tgagggtatt ctccacccgc tcctcctccc
     2281 atgaaacgag gtagaggatc ggatagcccg cttttacaaa attctctatg gttttcgtgg
     2341 ggaatgccat ggctgaacgt aagtatatag caagtccgtc tgattagtcg cgggaaaacg
     2401 gattctaatg gaataagagg acttacatcc cctgggatgt gactctcttc gggtgctctt
     2461 tacgccatct gagcttgttt agggcgttca cgtacgcttt cacgctcgcg acgactatat
     2521 cggtgttcga cccctggccc tgtgtgatga ccccttcgtc ttctaccctt accgtaacct
     2581 caccctgggc gtctatacct tcggtaatcg cagtgactac gtagctttct agcgtaggat
     2641 taagtccggt ggcttttgat acggcctgat atgccgcgtc cacgggcccg tccccgcttt
     2701 cggagatggt gacttccttc ccgtctatga gcagctttac ggtcgctacg ggctgcatgt
     2761 ccgagccgcc cgaatagttt gcggagatga gcttataaaa atccgacgaa cggacgaact
     2821 cttcgcttat gatagcttct atgtcctcat cgaacacgta tttcttcttg tcggccaggt
     2881 ccttgaactt cctgaacgcg ttctcgaacg acttatcgtc gaggaaatac ccataatcct
     2941 cgaggcggtc cttaaatgcg tgccttccgg agtgcttccc gagaatcagc ttgttagaag
     3001 gtatgccgac gtcttcaggg ttcatgattt cgtacgtgat cctttccttg agcactccgt
     3061 cctgatgtat gccggcctcg tgggcgaaag cgttcgcccc gactatcgcc ttattcggct
     3121 ggacactcac tccagttacc tgcgaaacga gcctgctcgt cggataaagc tggtccgtgt
     3181 tgatcctcgt cgtgaaaggg tttctgtcat tcctgacctt gagacccatc acaatctctt
     3241 cgagcgaggc gttccccgcc ctctctccga gaccgtttat agtgcactcg acctgtctcg
     3301 cgccttctct tatggcggcg atcgaattgg cgaccgcgag tcctaggtcg ttatggcagt
     3361 gcacgctcaa aacgatgttg tcgagccccg gcaccctctc cttcaacgtc cttatgatgt
     3421 acgaaaactc ctcgggcacg gtgtatccga ccgtgtccgg gatgtttatg attttggcgc
     3481 ccgatctcac cgcgacgtcg accgcacggc agaggtaatc gagctcggtc ctggtcgcat
     3541 cctcgcagga aaactccacg ttctcagtgt aatgtttcgc gtgctttacc gcggtgctga
     3601 ttatttcgag cacctcgtcc ctcgatttcc tgagcttatg tttcaagtgg atgtcggagg
     3661 tggcgatgaa agtatgtatc ctcggatact ccgctccctt cacggcctcc catcctctgt
     3721 ctatgtcgtt gaaattggcc ctgcacaggc ccgctatctc acagccgcgt atcttctcgg
     3781 ctatgatttt cacggattcg aaatcccctt cggaagaaat gggaaacccg gcttcgatga
     3841 catcgacgcc gagtttttcg agctgatggg cgaccctcag tttctcttcc gcggtcatgc
     3901 cgcagcccgg ggcctgctct ccgtctctca a
//
LOCUS       DASSJH010000302         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_932526, whole genome shotgun sequence.
ACCESSION   DASSJH010000302 DASSJH010000000
VERSION     DASSJH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932526"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1328
                     /locus_tag="VFJ67_06475"
     CDS             72..1328
                     /locus_tag="VFJ67_06475"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET7290069.1"
                     /translation="MDNAYSYLNSLGLHKIKPGLGRIKKLLESLGNPHNRVPGIIVGG
                     TNGKGSAAAAIASMLRAGGYRTGLYTSPHLIDVTERIRIYGREIERSELARILLRVKR
                     ASEKSTGGSASYFETLTASAFVYFAESGVDFSVLEVGMGGRWDATNVITPLVSVITNV
                     SKDHTDYLGKRIEEIASEKARIIKPGVPAVTCARGKALRVIAEYALRQGAPLRVYGRD
                     FKSSGESADSFDYTGSVWTLKGLSSNLSGHYQTENLSAAVSAVEALYEHHGIKLGEKS
                     LRKGLGAIEWPGRLETIRKHPPLILDSAHNPGGAKALAESLKLMYPSLRFTFLVGMLT
                     DKDHSSFINELVPIAGKFIATEIESERALSAEVLARKISRIFQGPVVVGKDYRKAYRE
                     LIDEGEPACIAGSLYLTGAIKALRGS"
     gene            1405..2439
                     /locus_tag="VFJ67_06480"
     CDS             1405..2439
                     /locus_tag="VFJ67_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET7290070.1"
                     /translation="MKKVGIALDKLYVDHDNGFGHPESQERLLAIIDMLKHTGLLDEV
                     SKIAPRDATKEEITLVHTPEHYDRMASTKGKPKVFLDADTSTCPVSFDAAVRAAGGVL
                     SAIESVLSGETDRAFPLVRPPGHHAESDRPMGFCLFNSVAVGGAYLTDVKGYERVMIV
                     DWDLHHGNGTQHMFYDTPKVLYFSTHQYPYYPGTGGPSETGHGEGKGYTVNVPLPPGM
                     GDKEYIKIFNEILGPVTEQYKPEFILVSAGFDTFYDDPLGGMKVTPEGFAKMTRFLTD
                     AAEKHSGGRIVFVLEGGYNLDGLWISTKEVIEELLDKKRSTYDSPEEPTGADLIIDRV
                     KKDYSPHWKF"
     gene            complement(2436..3263)
                     /locus_tag="VFJ67_06485"
     CDS             complement(2436..3263)
                     /locus_tag="VFJ67_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7290071.1"
                     /translation="MSGRKKGSVGCDDCHWSGFVVKNKGNFAAASLCSCVLECPECAG
                     VGNVLLTNEKGYSYVSPCPSCGVVRRNVRLYNTAGIPAKYSHVLQVDAGLVPTHINRH
                     LQMALKYAKEEFVKKYPTKKGFLLMGPSGLGKTHLAVGTISELTLYHGVRCMFKDFFY
                     LLSELKQAYSEGTPENEVILPLIETEVLVIDELGKGRSNEWELNILDQLISKRYNASR
                     MTLITTNYVTREIAKATGNSDEILEVRVGERIASRLFEMCELIHLEGRDYRREKKKV"
BASE COUNT          785 a          949 c          974 g          693 t
ORIGIN      
        1 atcaagcgca taaaccaggg gtacccccgg tttcatgata taatcccata actacgggta
       61 ataaacttac aatggacaat gcttacagtt acctgaattc cctcggcctt cacaagatca
      121 agcccgggct cgggcgtatc aagaagctcc tcgaatcgct cggaaaccct cataacagag
      181 ttccggggat aatcgtaggg gggactaacg gcaaggggtc tgcggcagcc gcgatagcat
      241 ccatgctcag ggccggtgga tacaggacgg gactatacac ctcccctcac cttatagacg
      301 tcacggaaag gatcaggatc tacggcaggg agatcgaacg ctccgagctc gcgcgtatac
      361 tgctgcgcgt aaagagagcg tccgaaaagt ccacaggagg gagcgcgagc tacttcgaga
      421 cgctcacggc ctcggctttc gtatatttcg ccgaatcggg cgtcgatttc agcgttctag
      481 aagtgggcat gggcggcagg tgggacgcga cgaacgtgat aacacctctc gtatcggtca
      541 tcacgaacgt atcgaaagac cacacggact atctcgggaa gaggatagag gagatagcct
      601 ccgagaaggc ccggataata aagccggggg tcccggccgt aacgtgcgcg agagggaaag
      661 cgctcagggt aatagccgaa tacgcactga ggcagggagc gccgctcagg gtttacggga
      721 gggactttaa atcgtcagga gagagcgcgg actcgttcga ttacacgggg agcgtgtgga
      781 cgttaaaggg cctgagctcg aatctcagtg gacattacca gacggagaac ctgtcagcgg
      841 ccgtctcagc cgtagaagcg ctgtacgagc atcacggaat aaagctcggc gagaagagcc
      901 tgagaaaggg gctcggtgct atcgaatggc cgggaaggct cgaaaccata agaaagcacc
      961 ctcccctgat actcgacagc gcgcacaacc ccggaggcgc gaaagcgctt gccgaatcgc
     1021 ttaagctcat gtacccctcg ctcagattca cgttcctcgt cgggatgctg acggacaagg
     1081 accacagctc atttataaat gagctggttc cgatagcggg gaagttcatc gccaccgaaa
     1141 ttgagtccga aagggctttg agcgcggagg tgctcgcccg gaaaatttcg aggatattcc
     1201 agggtcccgt cgtcgtcggg aaggattaca ggaaggcgta ccgtgaatta attgacgaag
     1261 gcgagcccgc gtgtatcgcc gggtctttat atctcacggg ggcgataaag gctctccgcg
     1321 gctcctgagg agcacattca ggctttgtct aaacccgttt ctgtgctagc cttttatccc
     1381 acgccaaaag tggactaaat cgcgatgaag aaagtcggaa tagctctcga caagctctac
     1441 gtagaccacg ataacgggtt cggacacccc gaatcccagg agaggctcct cgccattatc
     1501 gacatgctca agcatacggg gctcctcgac gaggtatcga agatcgctcc gcgagatgcg
     1561 acgaaagagg agatcacgct cgtgcacacg cccgagcact acgacaggat ggcgtccaca
     1621 aaagggaagc cgaaggtgtt cctcgacgcc gacacctcca cgtgcccggt ttcgttcgac
     1681 gcggccgtga gggccgccgg cggggtgctc tcggcgatag agagcgtgct ctcgggagaa
     1741 acggacaggg ctttcccgtt agtaaggccg cccggtcacc acgctgaatc agacaggccg
     1801 atggggttct gtttattcaa cagcgtcgcc gtcggaggcg cctacctgac cgacgtaaag
     1861 ggctatgagc gcgttatgat tgtggactgg gacctccacc acgggaacgg tacccagcac
     1921 atgttctacg acaccccgaa agtgctttat ttctcgacgc atcaataccc ctattatccg
     1981 ggcacgggag ggccgtccga gaccgggcac ggcgagggca agggatatac tgtgaacgtc
     2041 ccgctccctc cggggatggg tgacaaggaa tacataaaga ttttcaacga gatactcggc
     2101 cccgtgaccg aacagtacaa gcccgaattc atactcgtat cggcgggttt cgacacgttt
     2161 tatgacgacc cgctcggggg aatgaaggta acgcccgaag ggttcgcgaa gatgacgagg
     2221 ttcctcacag acgcggccga gaaacactcg ggcggcagga tcgtattcgt actcgaaggc
     2281 ggatataacc ttgacggcct ctggatctct accaaggaag tgatcgaaga gctcctcgac
     2341 aagaagcgct cgacgtacga ctcgcctgag gagcctacgg gggctgacct gataatcgac
     2401 agggtcaaaa aggactattc accgcactgg aagttctaga ccttcttctt ctctctcctg
     2461 taatccctgc cctcgagatg tataagctcg cacatttcga acaggcgcga tgctatccgc
     2521 tcccctaccc tgacctcgag tatctcgtcc gaattgccgg tcgccttcgc tatctccctc
     2581 gttacgtagt tggtcgtgat gagcgtcatc ctggaggcgt tatagcgctt ggaaattagc
     2641 tggtcgagta tgttgagctc ccactcgttg ctccgcccct tgccgagctc gtctatgacg
     2701 aggacctcgg tctctatgag cggcagtatg acctcgttct cgggggtccc ttcggaatag
     2761 gcctgcttga gttcggaaag gaggtaaaag aagtccttga acatgcatct cacaccgtgg
     2821 taaagcgtaa gctcggatat ggtccctacg gcgaggtgtg ttttccccag acccgaaggc
     2881 cccatgagca ggaagccttt cttggtcggg tacttcttta cgaactcttc cttggcgtac
     2941 ttaagcgcca tctgcagatg tctgttgatg tgcgtgggca cgaggcccgc atcgacttgc
     3001 aggacgtgcg aatacttggc cggtatcccc gccgtgttgt agagcctgac gttcctcctg
     3061 acgacgccgc acgaggggca cggcgagacg tacgagtacc ccttctcgtt cgtcagcagc
     3121 acgttcccga ccccggcgca ctcaggacat tcgaggacgc acgaacatag cgaggccgcg
     3181 gcgaaattcc ccttgttttt aaccacgaac ccagaccagt ggcaatcgtc gcagcctacg
     3241 gatcccttct tcctgccaga cattttcatg agccgtttac gagattatac actttattcc
     3301 gtattttttt gcgagaatct cgttcctgaa ggaaaccagg ctctcgtcgt atgcgctctt
     3361 agtcatgtag cgggccctcc gcctgatcat ctcctcggct g
//
LOCUS       DASSJH010000303         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1371750, whole genome shotgun sequence.
ACCESSION   DASSJH010000303 DASSJH010000000
VERSION     DASSJH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371750"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..494
                     /locus_tag="VFJ67_06490"
     CDS             267..494
                     /locus_tag="VFJ67_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290072.1"
                     /translation="MDIKIVPYTFDPTCDCSPDAVGSDKSLHIESVHAPITYWGVFLN
                     ENRVSTTSSRELAEKTKAWMENWLASPQERV"
     gene            886..1914
                     /locus_tag="VFJ67_06495"
     CDS             886..1914
                     /locus_tag="VFJ67_06495"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7290073.1"
                     /translation="MKAVVTGGTGFIGGRLVEELVSNGYGVRCLVRDTSDVKMLKELG
                     AELLHGDLGDKDSLERLPEGGDVVFHVAAHVTDWGPRDVFRRYNVDATETLLRASLRS
                     GVKRFVHMSSSTVVWKSDFWSVHNLTDIDETYPYRDRYNDYYNESKADAERLVLGFGR
                     KNGLGTVVIRPSNVWGAGDTVILPRVAMAARKGILYPMGSGERTVTPCHVDNLARAML
                     LAAGNEKAPGNIYFINDGVKISYTDFLSKQLKACGIEWKPGFSIPYKFAYSVAALLEF
                     IYRLRGSEKPPVLTRFAVAALAGSRSYSIEKARRELGYKPSVDLERGMKEMEDWVKLL
                     GGTDALLK"
     gene            1980..2711
                     /locus_tag="VFJ67_06500"
     CDS             1980..2711
                     /locus_tag="VFJ67_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7290074.1"
                     /translation="MRDALTENTYDIKAEGLKKSFGGNEALRGIDLHVKKGEFYTLFG
                     PNGAGKTTFIKILSTLAKPSSGSLTVAGHDAVKEPDSVRRVTGVLSHDPFLYENLSAF
                     ENIMFFGSLYGVEDPGGRAREVIDRVGLGKREHDLVRTFSRGMKQRLAVARAIVHDPG
                     ILLLDEPYTGLDQHGARVFGEMLSMLKSDRRTILMTTHNIDEGLDRSDRVGILAGGRM
                     VYESGPEGVGKEEFREIYISRAGNA"
BASE COUNT          692 a          708 c          816 g          577 t
ORIGIN      
        1 gaaaattttc tactataagt cggcaattgt caaacaattc aggaaaagtg aatgattgaa
       61 gggtattaca tggggagaat ccgagtatcc cggcttattc gggtcacgcg catgtcgctt
      121 ttttttgcac atttttgcat ctacgtcggt cgtggaattt gtaagccctt gaaatcaaag
      181 gattgatgat tagcgtgtat gttggcatgt aaattgcata tagatttaca tgagccgtaa
      241 aaaatttcga aaggaggtag gaaaaaatgg acattaagat agtgccgtat actttcgatc
      301 cgacgtgcga ctgttcgcct gatgccgtag gttcagataa aagcctccac atcgagagcg
      361 tgcacgctcc tataacgtac tggggagtat tcctgaacga gaacagggtc tcgacgacat
      421 cgtccaggga gcttgccgag aagacgaagg cgtggatgga aaactggctc gcaagcccac
      481 aagaaagagt ataaaatccg acaccccata ccagccgcat cgacgcaacc gctttacgtg
      541 cgccggtgcg gctctttagc ttctgaatat gctgaatatg ccgtaggacc tgaagttgtg
      601 tcgtgactga acggaaccct gtgtgaatat atgcgcttca atggattccg tctttccgac
      661 ttctccatct tcctttgcta aagcttcgga ggataaaaag ctacgtcgag cgggcagaat
      721 gaacaggtgt agtttgattt attaaactaa tgtacacccc caccctgtcc ctccccccct
      781 cctacacagg gggagggaat gcgcaccctt ttgactctcc acgccctttg gaagtatatt
      841 agtcctcatg ccctatagaa tttcagctcg cacggagcgg agataatgaa ggccgtcgtt
      901 acaggcggga cagggttcat aggggggagg ctcgtcgagg agctcgtatc gaacgggtac
      961 ggcgtcaggt gcctcgtgag ggacacgagc gacgtgaaga tgctgaagga gctgggagcg
     1021 gagcttcttc acggggacct cggagacaag gacagcctcg aaaggcttcc cgaaggggga
     1081 gacgtcgtct ttcacgtggc cgctcacgtg accgactggg ggccgcgcga tgtgttcagg
     1141 agatataacg tggacgcgac tgagacgctc ctgcgcgcgt cgctacggtc aggcgtgaag
     1201 aggttcgtcc acatgagctc ctctactgtc gtctggaagt ccgatttctg gagcgtccat
     1261 aacctcaccg atatcgacga gacctatccc tacagggaca gatacaacga ctactacaac
     1321 gaatccaagg cggacgccga gaggctcgtc ctcgggttcg gcaggaagaa cggcctcggc
     1381 acggtcgtca taaggccctc gaacgtgtgg ggagcgggcg acaccgtgat actcccccgc
     1441 gtcgccatgg ctgcgaggaa ggggatactc tatccgatgg ggagcgggga aagaacagtt
     1501 acaccgtgcc acgtcgacaa cctcgcccgc gcgatgctcc tcgccgccgg gaacgagaaa
     1561 gcgccgggta atatctattt cataaacgac ggtgtgaaga taagctacac ggattttctc
     1621 tcgaagcagc tcaaggcgtg cggcatagaa tggaagcccg gcttctcgat accgtataaa
     1681 ttcgcctact ccgtcgcagc gctcctcgaa ttcatataca ggctcagggg ctctgagaag
     1741 ccccccgtgc ttacgaggtt cgcagtcgcg gcgctcgccg ggagcaggag ctactcgata
     1801 gaaaaggcca ggcgcgagct cggctataaa ccgtccgtcg atctcgagcg gggtatgaag
     1861 gagatggagg actgggtgaa gctcctgggc ggcacggacg cccttttgaa atagcgcata
     1921 ggataagccg cagctgtttg aaagtaatat ccgtatgatg tagttttctc acctctgaca
     1981 tgcgggacgc actcacggaa aacacatacg atataaaggc cgaagggctc aaaaagtcct
     2041 tcgggggcaa cgaggctctc cgcggaatag acctacatgt aaagaagggt gagttctaca
     2101 cgctcttcgg ccctaacggc gccgggaaga cgacgttcat caaaattctc tccactctgg
     2161 ccaagccttc gtccggcagt ctcacggtgg ccgggcacga cgccgtgaaa gaacccgaca
     2221 gcgtgagacg cgtgacaggg gtgctttcgc acgacccttt cctctacgag aacctgagcg
     2281 cgttcgagaa catcatgttt ttcgggtcgc tctacggggt tgaggatccc ggcgggaggg
     2341 cgcgcgaggt catagacagg gtagggctcg ggaagcgtga gcacgaccta gtaagaacgt
     2401 tcagcagggg catgaaacag aggctcgccg tcgcgagggc gatagtgcac gaccctggaa
     2461 tacttctcct cgacgagcct tacacggggc tcgaccagca cggtgcccgg gtattcggcg
     2521 agatgctctc catgctgaaa tcggaccgca ggacaattct catgaccacg cacaacatag
     2581 acgaagggct cgaccggagt gaccgcgtcg gcatcctagc cgggggcagg atggtgtacg
     2641 agagcggccc ggaaggggta ggcaaggagg agttcaggga gatatatata tccagagccg
     2701 ggaatgcctg atccggttga taaccgccga tgtgctttaa catatttatg agatatatga
     2761 taataaaaac agtctataag aacatatagt taa
//
LOCUS       DASSJH010000304         7225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_609209, whole genome shotgun sequence.
ACCESSION   DASSJH010000304 DASSJH010000000
VERSION     DASSJH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609209"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFJ67_06505"
     CDS             <1..283
                     /locus_tag="VFJ67_06505"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET7290075.1"
                     /translation="RSASSVIRRMLDAGEIDAVTFTSSSTVRNFLAIFPGFKPVRGKP
                     ALACIGPVTEKTLRDSGLRAAITSDEYTVEKLARAIAGHFVKSGRRKIK"
     gene            352..1074
                     /locus_tag="VFJ67_06510"
     CDS             352..1074
                     /locus_tag="VFJ67_06510"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET7290076.1"
                     /translation="MPRFPVDGKKIENGKAFLSGTDYRHVVKVLRMKEGDAITLFDES
                     SVEYEGRITQVGSKEVVVDIVSSRKVETDSPLRITLMQGLPKGDKMDYIIEKATEIGV
                     HTIVPVVTERSQVRTIEKKTRWERIAVEASKQCGRTKPPAIENTLNFDEAIKHKDNNE
                     LALILHVGSQVSLKDFLKNPLQHPTNIIVLIGPEGGFSEKEVLLATEMGFTSLGLGPR
                     TLRTETAGIAVLSIIQFQHGDL"
     gene            complement(1075..2550)
                     /locus_tag="VFJ67_06515"
     CDS             complement(1075..2550)
                     /locus_tag="VFJ67_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7290077.1"
                     /translation="MQVRFSALMVLAYLLAQIFICGSYATIAHGQQNPHNMETMGKDQ
                     FIENRCVRCHTIGRGRFVGPDLSGVGERYTREDLIKWIENPQQVYQATGKMPVNEGYP
                     PMPPMDIHPMAAQAIADYLLTVKVRPDTGKGGTITGKVVDKTNDKAVSGAALRLTSFM
                     GDKETGSADSTTEEDGSFTFESLPWDRSYMVTVEHNGAEYSTDKLVFYPDEDVKTLDL
                     PVYDPTDSSAGISIAEAHMIVQVVEDGLSVADLSAFDNKGDKMYVGGAELADGKKETL
                     KFSVPKDAANLNFIHGLTPEGVVRTETGFSDTASVPPGPKRVVFAYTMPLGSGSGTLE
                     KTIEYPTASFLLLVSKTNDQVTVDGLDGGDSVKIENEDFIRWSGENLQPGHRITVKFV
                     NPGALKDNIIKWGGIAVLIIAVGGGILYSSLARGKSRETAGEKPAGSRESLIMRRSAL
                     IKEIAALDDGYEAGTIDESSYREIRDSKKAELVEVTRRLRF"
     gene            complement(2534..3082)
                     /locus_tag="VFJ67_06520"
     CDS             complement(2534..3082)
                     /locus_tag="VFJ67_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7290078.1"
                     /translation="METIPALVIALLAAIFISLPFFLRTGGRSGDEYFPDARAEKFKE
                     LNSRKDSLLTAIKDIEFDYGLGKLSRDDFEELNGKYRIEAASLLKEIDSIGSAGTAPA
                     DKFDDDIEKEIRAERKKFLAPYDDEEIEKEILRAREASWAEAADRYCPKCGSGCGADD
                     LYCSRCGAKLSPLTENRNASPL"
     gene            complement(3130..5136)
                     /locus_tag="VFJ67_06525"
     CDS             complement(3130..5136)
                     /locus_tag="VFJ67_06525"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET7290079.1"
                     /translation="MVETGKISVILAFAISVYVVFASVYGAKTGKSDFVKSARNGVYA
                     VFFLLLIATIALLHSLVNLDFSLKYVALNTSTDLPVVYRFTSLWAGQAGSLLLWALIL
                     SIYSALVVLDTRSRSRLLMPYVIAVLAAISVFFTFIIGFVESPFETLPVAATEGRGLN
                     PILQNAYMAIHPVTLYLGYVGVSVPFAFGMGALLSGQLGDDWIKFSRKWTLFAWTFLS
                     VGLLLGARWAYLELGWGGYWAWDPVENAAFMPWLAGTAFLHSVMIQEKKGMLKKWNMT
                     LIIITFFLAIFGTFITRSGVISSVHSFAQSDIGPLFLGFIAFILLFSFAMFLYRVRDL
                     ESENKFDSILSRESAFIFNNLLFLGAAFAVFLGTVFPIMSEALTGHKILVGSPYFNKV
                     NVPIALVLIFLMGVGPLISWRKASKENLIKNFLAPVVIGIITAAVLIALGVRDVYALL
                     SYALCAFVAGTVFTEFFRGVSVRSRRGENPALAFVRLVSRNKRRYGGYIVHIGVVIIV
                     LGITSSSVFVTEKQATLTKGQSVELGGYMLTFQGIEQYTTDAKNATVAKLDGYKNGKP
                     IGDVSPEKNVYKYEGNREINQETEVALRSTFTDDLYVILMNYGEDGTATIRVMVNPMV
                     SWIWSGGVVLLIGAIVTMWPGGRKEREEVFVRYTLHGRDTTAKV"
     gene            complement(5136..5573)
                     /locus_tag="VFJ67_06530"
     CDS             complement(5136..5573)
                     /locus_tag="VFJ67_06530"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET7290080.1"
                     /translation="MLKGKLKFIIAIAVIALAVSYLVYGGVRDTMVYYLTVKELKSRV
                     PSVYGERVRVSGKVLPGSIKHGPDGSLEFSIYDGGGNINVAYSGIVPDIFKDDVEAVV
                     EGAYGKDNVFSADVLLAKCPTKYESTEALYKNTGPGDKGVKTY"
     gene            complement(5627..6286)
                     /locus_tag="VFJ67_06535"
     CDS             complement(5627..6286)
                     /locus_tag="VFJ67_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290081.1"
                     /translation="MKEKLFLILMVPLFLAAASCYDSAEKDTHITRFPVSGPGELKSI
                     DSPVIDRSVTSDGNGSLLVETDSPVSAVLYKLEGAGMEHARAAYKAMLRTEDLTSVGG
                     SRGIAYLEMKVVYPGGKEVVSRGPAVPPTGTTEWTPAETVLYSDKGTAPESVTLSLVV
                     DGKGKVWIDDVMLVTQPLRLDYLMWGHAVVWIVLIIYIYYLLDKQKRLRLELQALGRN
                     T"
     gene            complement(6464..7126)
                     /locus_tag="VFJ67_06540"
     CDS             complement(6464..7126)
                     /locus_tag="VFJ67_06540"
                     /inference="COORDINATES: protein motif:HMM:NF025255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HET7290082.1"
                     /translation="MNIHAEMYDYYIMAAVAVGLIECFFGYRILRFLLGVLGFIAGAV
                     VCGSLGYELTDGSEITSIIAGIAGGLAGAFIMYTLYIVGVFAIGAALGFMTASYVFGI
                     MNAAPATIVLIASAVSGGVLAAILQKPMLILATAFGGAYAAVTGVAYLLLRNFDPLDP
                     EFLNTLGEDQLYRMAIIWFGLGVFGFVIQLMTLPRKEEPVPEGEDAGGEEETHGDAGE
                     EG"
BASE COUNT         1575 a         2093 c         1951 g         1606 t
ORIGIN      
        1 aaggagcgct tcgtccgtaa taagaagaat gctcgacgcg ggcgagatag acgcagtcac
       61 cttcactagc tcgtcgaccg tgagaaactt cctcgccata ttccccggat tcaaaccggt
      121 gcgcgggaag cctgcgctcg cgtgcatagg gcccgttacg gagaagacgc tccgtgactc
      181 gggcctccgc gccgcgatca cgtccgacga atatacggtg gagaagctcg cccgggcgat
      241 cgccgggcac ttcgtaaaga gcgggcgcag gaagattaag taaaccgcgg atcgcggaaa
      301 gcactactca catcctcaaa ttgcatttgc aactgtctca ctggaataac gatgccaaga
      361 tttcccgtcg atggaaaaaa gatagaaaac ggaaaagcgt ttctctcggg gaccgactac
      421 aggcacgtcg tgaaggtgct tcggatgaag gaaggcgacg cgatcactct cttcgatgag
      481 agctcggtcg aatacgaggg gaggatcacg caggtaggat cgaaagaggt cgttgtcgac
      541 atcgtctcgt cgcgcaaggt agagacggat tcacccctcc gcataactct catgcagggc
      601 ctccccaagg gggacaagat ggattacata atcgagaagg cgacagagat cggcgtacac
      661 acgatcgtcc ccgtcgttac ggagaggtct caggttagga cgattgagaa gaagacaagg
      721 tgggagagga tcgcggtcga ggcttccaag cagtgcggaa gaacgaaacc gccggccata
      781 gaaaatactt taaattttga tgaggccata aaacataaag acaacaatga gttagcatta
      841 atactacatg ttggcagtca ggtatcattg aaagattttc taaaaaaccc tttacaacat
      901 cccacgaata ttatagtatt gataggaccg gaaggtggat tttcggaaaa agaagtcctc
      961 cttgctacag aaatgggttt tacctccctg ggtctgggtc ccaggactct tagaacggaa
     1021 accgcgggaa tcgcggtcct ttctataatc cagtttcaac atggagactt gtgatcaaaa
     1081 tcgcagtctc cgtgtaactt ctacaagctc ggccttcttg gaatccctta tttccctgta
     1141 actcgattcg tctattgttc ccgcctcgta accgtcgtcg agagcggcga tctcctttat
     1201 gagcgcgctc cttctcatga tgagcgactc cctgcttccc gccggctttt cccccgcagt
     1261 ctcacggctc tttcccctcg cgagcgacga atagagaata cctcctccga ccgcgataat
     1321 gagaaccgct attccgcccc atttgatgat attgtccttg agcgctccgg ggttgacgaa
     1381 ttttacggtg atcctgtgcc ccggctggag gttctctccc gaccacctga tgaaatcctc
     1441 gttctctatc ttcaccgagt cccctccgtc gaggccgtcc acggtaacct ggtcgtttgt
     1501 ctttgagacg agcagaagga agctggcggt cggatattcg atcgtcttct caagcgtgcc
     1561 gctgccggag ccgagaggca tggtgtatgc gaatacgact ctcttgggcc cgggcgggac
     1621 gctcgccgtg tcggagaaac ccgtctcggt cctgaccact ccctcgggag tgagcccgtg
     1681 tatgaagttg aggttggcag cgtctttcgg gacgctgaat ttcagcgtct ccttcttgcc
     1741 gtccgcgagc tccgcgccgc ctacgtacat cttgtcgccc ttgttgtcga atgctgagag
     1801 gtcggcgacg gagagaccgt cctccacgac ctgaactatc atgtgcgcct ccgctatgct
     1861 tatcccggcg ctgctgtcgg taggatcgta aaccggcagg tcgagcgtct ttacatcctc
     1921 gtccggataa aagacgagct tgtcggtcga gtactcggcc ccgttgtgct cgacggtgac
     1981 catgtagctc ctgtcccagg gaagcgattc gaaggtgaac gagccgtctt cctccgtcgt
     2041 tgaatcggcg cttccagtct ccttatcgcc catgaaggac gtgagcctga gggcagctcc
     2101 cgaaaccgcc ttgtcgttcg tcttgtcgac caccttgccg gtaatagttc cgcctttccc
     2161 ggtgtcgggc ctcaccttca ccgtgagcag gtagtccgca atggcctgtg ccgccatggg
     2221 gtgtatgtcc atgggcggca tcgggggata gccttcgttg acaggcatct tcccggtcgc
     2281 ctgatagacc tgctgcgggt tctctatcca tttgatgagg tcctcgcgcg tgtatctctc
     2341 gcccactccc gagaggtccg gccctacgaa cctgcccctg cctatcgtgt gacacctgac
     2401 gcagcggttc tcgatgaatt ggtccttccc catcgtttcc atgttgtgcg ggttctgctg
     2461 gccatgcgct attgtggcgt aagagccgca aatgaaaatt tgcgcgagta aatatgcgag
     2521 caccatgagt gcgctaaagc ggacttgcat ttcggttctc cgtcagtgga cttaatttgg
     2581 ctccgcacct ggagcagtag aggtcgtccg cgccgcagcc cgatccgcac ttcggacagt
     2641 acctgtccgc ggcttcagcc cacgaggcct ccctcgcgcg gagtatttcc ttttcgatct
     2701 cctcgtcgtc gtacggcgcg aggaacttct tcctttcggc gcgtatttcc ttctctatat
     2761 cgtcatcgaa cttgtcagcg ggtgcggtcc cggccgaccc tatcgaatct atctccttca
     2821 ggagagaggc ggcttctatc ctgtacttcc cgttgagctc ctcgaaatcg tcgcgggaca
     2881 gtttcccgag cccgtaatcg aactctatgt cttttatcgc cgtaagcagg gagtctttcc
     2941 tggagttaag ctctttgaac ttttcggcgc gcgcgtcggg gaaatattca tcgcctgacc
     3001 gtccgccggt ccttaagaaa aagggcagtg agatgaaaat agccgccagt agagcgatga
     3061 ccagagccgg tattgtttcc aaatcttttt ccttgtgtga atagcttaaa gtaaagcagc
     3121 cgggagcttt tataccttcg cggtcgtgtc cctgccgtgg agagtgtacc tgacgaagac
     3181 ctcttcccgc tctttcctgc cgccgggcca catggtcact atggccccga tcaggagcac
     3241 tacaccgccc gaccatatcc agctcaccat cgggttcacc attacgcgta tcgtcgccgt
     3301 gccgtcctct ccgtagttca tgagtatcac gtacaggtcg tctgtgaacg tggacctgag
     3361 cgccacttcc gtctcctggt ttatctcgcg gttcccctcg tacttataaa cgttcttctc
     3421 gggcgaaacg tcccctatcg gttttccgtt cttgtacccg tcgagcttcg ccacggtcgc
     3481 gttcttggcg tcggtcgtgt actgctcaat gccctggaac gtgagcatgt atccgccgag
     3541 ctcgaccgac tggcctttcg tgagggtcgc ctgtttctcc gtcacgaaaa cggaggacga
     3601 agtaatgccg agcacaatta tcacgacgcc tatgtgcact atgtagccgc cgtacctgcg
     3661 cttgttccgg gagacgagcc tgacgaaagc gagcgccggg ttctcccctc tcctgctcct
     3721 caccgagacg ccgcggaaga actccgtgaa caccgtgccc gcgacgaacg cgcagagtgc
     3781 gtacgagagc agcgcgtaaa cgtccctcac gccgagagct atgaggacgg ccgccgtgat
     3841 tatcccgatg acgacgggag cgaggaagtt ttttatgagg ttttctttcg aggccttcct
     3901 ccacgagatg agcgggccca cgcccatgag gaaaatgagc acgagcgcta tggggacgtt
     3961 caccttgttg aaatacgggg agcccacgag tatcttgtgc cccgtaagcg cttcggacat
     4021 tatcgggaat acggtcccga ggaatacggc gaaagccgca cccaggaaaa gcaggttgtt
     4081 gaatatgaac gcgctttccc gggagagaat ggagtcgaat ttgttttccg attcgaggtc
     4141 ccttacacgg tagaggaaca tcgcgaagga aaagagcagt atgaaagcga tgaagccgag
     4201 gaagagcggg ccgatatcgc tctgcgcgaa cgaatgcacg gaggatatga cacccgacct
     4261 cgttatgaac gtgccgaata tagcgaggaa gaacgtgatt atgatgaggg tcatgttcca
     4321 tttcttgagc atgccctttt tctcctggat catgaccgaa tgaaggaacg ccgtccccgc
     4381 cagccacggc atgaaggccg cgttttcaac ggggtcccac gcccagtacc cgccccaccc
     4441 gagctctaga tatgcccagc gcgcgcccag gaggagcccg acgctcagga acgtccaggc
     4501 gaagagagtc catttgcggg agaacttgat ccagtcgtcc ccgagctgcc cgctgagaag
     4561 cgctcccatt ccgaacgcga aaggcacgct cacgccgacg tagccgagat acagcgtgac
     4621 cgggtgtatg gccatatagg cgttctggag tatggggttt aaccccctgc cctcggtggc
     4681 ggccacgggg agggtttcga acgggctctc gacgaatcct attataaaag tgaagaagac
     4741 ggaaatcgct gcgaggacag caatgacgta aggcatgagg agcctcgacc tcgatcgcgt
     4801 gtcgagaacg acgagcgccg aatatatcga cagaatgaga gcccagagga gaagcgagcc
     4861 cgcctgcccc gcccagaggg acgtgaacct gtatacgacc gggaggtccg tgctcgtgtt
     4921 gagggctacg tatttaaggc tgaaatcgag gtttacgaga gaatgcagga gcgcgatagt
     4981 agcgatcaaa aggaggaaaa agacggcata cacgccgttc cgtgcgctct ttacgaaatc
     5041 gctctttccg gtcttggcgc cgtaaacgga cgcgaagacc acgtacactg atatggcgaa
     5101 agccagtatt acggaaattt ttccggtctc taccatcagt aggtcttaac ccctttgtcg
     5161 cccgggcctg tgtttttgta taacgcctcg gtcgactcgt atttggtcgg gcactttgcg
     5221 aggagcacgt cggctgagaa cacgttgtcc ttaccgtatg cgccttccac aaccgcctcg
     5281 acgtcgtcct tgaatatgtc gggcactata ccggagtatg cgacgtttat attgccccct
     5341 ccgtcatata tgctgaattc cagagagccg tccgggccgt gctttatcga gccgggcaga
     5401 accttgcccg agaccctgac cctctcgccg taaacggaag gcaccctgct cttgagctcc
     5461 ttgaccgtaa ggtaatagac catcgtgtcc cttacgccgc cgtaaacgag ataactcacc
     5521 gcgagcgcaa tgaccgctat ggctattatg aactttaatt tccctttaag cattgcctca
     5581 ccctctgaag atacacattc accgtatctc cgcgttacac accttcttaa gtattcctcc
     5641 cgagcgcctg aagctcgagc cttagcctct tctgtttatc gagcaggtaa taaatatata
     5701 taatgagcac tatccagaca accgcgtgcc cccacatgag gtagtcgagc ctgaggggct
     5761 gtgtgacgag catgacgtcg tcgatccaga ccttcccctt cccgtcgact acgagcgaga
     5821 gcgtgacgct ttcgggcgcc gtgcctttat ccgaatacag gacggtctcg gccggcgtcc
     5881 attccgtggt ccccgtgggc ggtactgcgg ggccgcgcga gacgacctcc tttcccccgg
     5941 ggtaaacgac cttcatctcg agataggcga tgccgcggga gccgccgacg gacgtaaggt
     6001 cttccgtcct gagcatcgct ttataagccg ctcgcgcgtg ctccataccg gcgccctcga
     6061 gcttgtacag caccgcgctc acgggcgaat cggtctcgac gagtaaagag ccgttcccgt
     6121 cggaggtcac gctcctgtct atcacgggcg aatcgatcga cttcaactct cccggaccgg
     6181 aaacgggaaa cctcgtaata tgcgtatcct tttccgcgga atcgtagcac gaagcggcag
     6241 caaggaagag cgggaccatc agaataagaa acagtttttc tttcacgccg gaacgaatca
     6301 tctactccct ctaagatgat acccgcatgg ccgggttatt aaaacaggat tctctccgcc
     6361 cgggccagac gccgcaaaca tttagcaatc ctaagtttca cggataactg taaaacggtc
     6421 aatgactaat tttagtaata atgacggtga ggtttgcccg tcctcacccc tcttcgccgg
     6481 cgtccccgtg agtttcctcc tcgccgccag catcctctcc ctccgggacc ggttcttctt
     6541 tccgtgggag cgtcatgagc tgtatcacga agccgaagac gcctaacccg aaccatatta
     6601 tcgccatcct gtagagctgg tcctccccga gcgtatttag gaactccggg tcgagcgggt
     6661 cgaagttcct gagaagcagg taagcgacac ccgttacagc cgcgtacgcc cctccgaaag
     6721 ccgtcgccag tatgagcatc ggcttttgca gtatcgcggc cagcacgcct cccgaaacgg
     6781 ccgaggctat gagcactatc gtcgctgggg ccgcgttcat aattccgaac acgtaagagg
     6841 cggtcataaa gccgagcgcc gcccctatgg cgaacacgcc aacgatatag agcgtataca
     6901 tgataaaggc cccggccagg cccccggcga taccggctat tatggaggtt atctcgcttc
     6961 cgtccgtgag ctcgtaaccc aggctcccgc ataccaccgc ccccgcgata aaacccagaa
     7021 cgcccaggag gaacctcagt atcctgtagc cgaagaagca ttcgatgagc ccaacggcca
     7081 cagccgccat tatgtagtag tcgtacatct cggcgtggat attcataggt gcatatccgc
     7141 atgaaaatcg aggaataatg aatatatacc aaataatgat agcatattac tgtgcattat
     7201 taatgtatat aatcagcgtc cgggg
//
LOCUS       DASSJH010000305         5708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1258717, whole genome shotgun sequence.
ACCESSION   DASSJH010000305 DASSJH010000000
VERSION     DASSJH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258717"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..622)
                     /locus_tag="VFJ67_06545"
     CDS             complement(26..622)
                     /locus_tag="VFJ67_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7290083.1"
                     /translation="MIYLKREYESGFTLIELLVVAAIIGILLAIAIPNLLKARISSNE
                     ANARKALQTLKDAEYEYFEQDLDGNGDRDFTNLIGTLGTAGTLRDPSGTGDPEDSLID
                     STFELAISSDGSPSSSADCIDPKAGYCLSWSSDAATDDQALRGDFGWEASMTSARVNG
                     RKDFSAYADKVIKCTATSQASGAAGTFESGFDDPACDG"
     gene            complement(963..1544)
                     /locus_tag="VFJ67_06550"
     CDS             complement(963..1544)
                     /locus_tag="VFJ67_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7290084.1"
                     /translation="MLDTLKRVKRGEKGFTLIELLVVAAIIGILLAIAIPNLIKARMS
                     ANEANARKSMQTLRDAEGEYFEQDLNNDGERNFTSNIAKLRDPEGSGDEVDALVDSSF
                     DDAVCGAGSTDGSCCTDPKAGYCITWDSAEVAEGDDLDDIAWQASMTSFNKTGRRDFS
                     VYGDGAIRCTISTVGTAGTPGQFESNRDTPGCD"
     gene            1878..2408
                     /locus_tag="VFJ67_06555"
     CDS             1878..2408
                     /locus_tag="VFJ67_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290085.1"
                     /translation="MFILKHKVCTLAVSLTVFALFISGCTTIKTTSYFTPTKTKLRDY
                     QALQFETFETKIEDFPKDALTKIPDKAAALLESENKFKEVKYGEIENIPAEDTIVLLG
                     EVSEYRPSSDVSFEGGALKFGEVSIAIKIALVNKATGDEIATGEVNGFSSTGFMAKDI
                     FQSLAEEVVKYVNETY"
     gene            2453..3466
                     /locus_tag="VFJ67_06560"
     CDS             2453..3466
                     /locus_tag="VFJ67_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7290086.1"
                     /translation="MRKSIFYLLLLAVAVCTFQAGAIGAAAQTASGAAGLYQQGRAEF
                     LKFTPDGFEKAIALYNQAIKADPKYAQAYAGLAEVYSFMGFYRYQVREEYETYYNNSY
                     ANMAKALQINPNLEETQLALAYSYLQLSREKDAIAEAQKILAKNPNNPEAMYILWSAS
                     GEKPDSPDIRKVIELDPKFVPAYIGLGNALFFKKRAYGQASEYFRKAAEIAPSAQIHN
                     LFGTALRTQGHYNEAIVEYQKAIKENPNYAPAYMNLGITYYYLNKFNQNIDNQKKAIS
                     LNPNYPDAYFFIAQGYERANNPQQAIVYYKKFLEVSLEQEMYAGYAAQAKQRLSALSA
                     GGK"
     gene            3554..3886
                     /locus_tag="VFJ67_06565"
     CDS             3554..3886
                     /locus_tag="VFJ67_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290087.1"
                     /translation="MPLEIDGHGSIMLSFGMESSASLWNIIFEGFDQEVVLVSKFSGT
                     DDRVVSTLYEFGEIIGKNYKDCKVKTTRTGIEIKKPLPRNDVSLVNEWTCLMESIRDE
                     MTKFIPAR"
     gene            complement(3902..4702)
                     /locus_tag="VFJ67_06570"
     CDS             complement(3902..4702)
                     /locus_tag="VFJ67_06570"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET7290088.1"
                     /translation="MTSPMIRKLNGLLKTETIGKVIHYFDETGSTNTVLSRLAEQGAS
                     EGTAVIADRQTSGKGRLGRKWFSPPGMNLYISILFRPSIPACESPLLTFLASVALVET
                     IKKTGVRNTSIKWPNDVLIDGKKAAGVLTEMSPRGERVDYIVVGVGVNINMTRAEIKR
                     EMGETARFATSLKESLGADIDRAKFTGDFLLEIESWYQTFNRRGKTSIIREWTERWGG
                     YNKRVRVAKEDGNLVEGIATGIDGEGHLLIKKDDGSAVSIITGDVSVI"
     gene            4879..5292
                     /locus_tag="VFJ67_06575"
     CDS             4879..5292
                     /locus_tag="VFJ67_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290089.1"
                     /translation="MSNILKICGIAAAAVALVLPLQSLSRAEQQPAAIDSPVIEVRIK
                     DVVSNPGEYNGKKVSLEGKVAKVDYRNSSKGEPFTIFKLKDSENNEVGVYFEDQRLPL
                     SKGDRVKIMGRFWKEKSYFLYKIKNVIKAKTVDII"
     gene            5391..>5708
                     /locus_tag="VFJ67_06580"
     CDS             5391..>5708
                     /locus_tag="VFJ67_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290090.1"
                     /translation="MTLLGNGLKRVLLFVILLVVVILVVLNIYLSGKLRQLAPAYLDR
                     YSKLSGLTLRADEAGLDPFFRIRLSGVKAENPSLPQNGLAEIKTLTVDPGIISSLFNR
                     RITI"
BASE COUNT         1396 a         1447 c         1458 g         1407 t
ORIGIN      
        1 catggccgga gtgtgtacgt cttcactatc cgtcacaggc cgggtcgtcg aaacctgatt
       61 cgaaagtgcc cgccgctccg gacgcctggg atgtcgcggt gcatttgatt actttatccg
      121 cgtatgcgga gaaatctttc ctgccgttta cacgggcgct cgtcatcgaa gcctcccatc
      181 cgaagtctcc acgtaatgct tgatcgtcgg tcgctgcgtc gctgctccag gagaggcagt
      241 atccggcttt gggatcgatg caatcggcgg agctcgaagg gctgccgtcg cttgagatcg
      301 cgagctcaaa ggtggagtct atgagcgagt cctcggggtc gcccgttccc gacgggtccc
      361 gtagcgttcc tgccgttccc aaggtcccta tcagattcgt gaagtccctg tctccgttgc
      421 cgtcgaggtc ctgctcgaaa tactcgtact ccgcgtcttt taaggtttgg agcgccttcc
      481 gtgcatttgc ctcgttcgat gatatacggg ctttcaagag gttggggatc gcgatagcca
      541 gcaggatgcc tatgatcgcc gctacgacca gaagctcgat cagtgtaaag ccggattcgt
      601 actccctctt caaatagatc atataaagtc gtccacttgt ataaatagca atatgcgtac
      661 caattaataa ttgaaacagt taaagtacag tttttgtaag accttggtat cattataaat
      721 gctgatgacc gattcaacat gtattcgttt gttctaaatt ggaagattgg gtaaaacaaa
      781 aatgtgacaa aaattgtcac attgacgcat tttgtactct acggcagatc ggcgccgagc
      841 ttatgaatgc catctatgat gtgatggtat aaaggatgaa aactctctga ggcaaaaaag
      901 cggcgggtcc gtttgaaccc gccgcttttt aatatcacaa ttagtttaaa gcttgaattg
      961 gattagtcgc atcctggtgt atcgcggttg gactcgaatt gtccgggtgt accggcggtc
     1021 ccgactgtgc tgatcgtgca tcttatcgct ccgtcaccgt aaaccgagaa gtcccttctg
     1081 cctgtcttgt tgaagctcgt catgctcgcc tgccaggcta tgtcgtcaag gtcgtcccct
     1141 tcggcgactt cggctgaatc ccaggttata cagtatccgg ctttagggtc ggtacagcag
     1201 ctcccgtcgg tcgagcctgc tccgcataca gcgtcgtcga agctgctgtc gacgagcgcg
     1261 tccacttcgt caccactgcc ttccggatcg cggagttttg caatattact ggtgaagttc
     1321 ctctcgccgt cgttgttgag gtcctgctcg aagtattcgc cctcagcgtc cctgagcgtc
     1381 tgcatggact tcctggcatt agcctcgttc gccgacattc tcgcctttat aaggttagga
     1441 attgcgatcg caagcagtat gcctatgatt gccgctacta ccagaagctc gatcagggtg
     1501 aaacccttct ctcccctttt aactctcttt aatgtgtcta acatgaaaat aacctccttt
     1561 ttcggaaatg agtcttgggg atatagtttg caaagcccgt gccacacagc cgggtatagg
     1621 atgttattct tgtaacaccc gggatttatt gaaaaactac aaattcctca gggttgttga
     1681 cattaatttc ggtttttcat tcggtatcag attttgggat tcgtgacact ttttagtcga
     1741 tttgtccaca atgtgacaaa tattgtcagt atatcccttt cagtaaccat tgcggatggt
     1801 aaaatgaatc gatatttatc aatcggagga agtatgctat tttatactac ctatttagaa
     1861 aggaggcgga ttaaatcatg tttatattga agcataaggt ctgcacactg gcggttagcc
     1921 tcacggtatt cgccctgttt atttcggggt gcaccactat aaagaccact tcctacttta
     1981 ccccgacgaa gactaaattg cgtgactacc aggcactcca gttcgagacc ttcgagacaa
     2041 agatagagga cttcccgaaa gatgccctga cgaaaatacc ggacaaggct gccgcgctcc
     2101 tcgaatcaga gaacaagttc aaagaggtca agtacgggga aattgaaaat atccctgctg
     2161 aggatactat agtactgtta ggcgaggtta gtgagtacag gccgagctcg gacgtttcgt
     2221 tcgaaggcgg ggcgcttaaa ttcggcgagg tatcgattgc tataaagatt gctctcgtca
     2281 acaaagcgac aggagacgaa atcgcgacag gagaagtaaa cggtttcagc tcgaccggct
     2341 ttatggcgaa agatattttc cagagccttg cagaagaggt cgtcaagtac gtaaacgaaa
     2401 cgtattgagc gtgcccggaa gttcagttcg gaaataaatc agggaggatg atatgagaaa
     2461 atctattttt tatcttttgc ttcttgccgt ggccgtttgc acattccagg cgggagcaat
     2521 cggggcagcg gcgcagaccg cgtcaggcgc agcgggcctt tatcagcagg gcagagcgga
     2581 gttcctgaaa tttacacccg atggattcga gaaggctata gccctctaca accaggccat
     2641 aaaggcggac cccaaatacg cgcaggcata cgcagggctt gcggaggtgt actcgttcat
     2701 gggtttctac aggtatcagg taagggagga atatgagacc tactacaata attcatacgc
     2761 caacatggca aaggcgcttc agatcaatcc gaacctcgaa gagacacagc tcgcgcttgc
     2821 atacagctat ctccagctga gccgcgagaa agacgcgatt gcggaagcgc agaagatact
     2881 ggccaaaaat ccgaacaacc cggaagccat gtacatccta tggtcggcaa gcggggagaa
     2941 acccgactcg cccgatataa gaaaggtgat agaactcgat ccgaagttcg taccggcgta
     3001 cataggtctc gggaatgcgc tcttcttcaa aaagagggcg tacggacagg cgtccgaata
     3061 tttcaggaag gccgccgaaa tcgcaccgtc ggcgcagatc cacaacctgt tcggcacagc
     3121 gctcaggacg cagggtcact acaacgaagc tatagttgag taccagaagg ccataaaaga
     3181 gaacccgaac tatgcgcccg cgtacatgaa cctcggcatt acgtattatt acctcaacaa
     3241 gttcaaccag aacatcgata accagaagaa agcgatatcg cttaacccga actaccccga
     3301 tgcgtacttc tttatcgcac agggatatga aagggccaat aaccctcagc aggcgattgt
     3361 gtactacaag aaattcctcg aagtctctct tgagcaggag atgtacgcgg gctacgcggc
     3421 gcaggccaaa cagaggcttt cggccctgag tgccggcggc aaataacgcg ggctgagtgt
     3481 ttttagctgt gacagcaatg tttcgaccat agagaagggc ggaatctgag taagacgggg
     3541 caggtccaac gatatgccgc ttgaaattga cggacacggg tcgatcatgc tctccttcgg
     3601 catggagtcg agcgcgagcc tgtggaatat tatattcgag gggttcgatc aggaggtcgt
     3661 actggtatcg aagttctcgg gaacggacga ccgggtagta agcacgcttt acgaattcgg
     3721 ggaaatcatc ggaaagaatt acaaggactg caaggtcaag acgacgcgta ccggtatcga
     3781 gataaaaaag cctctcccga ggaacgatgt gtcgcttgtg aacgaatgga cctgtctcat
     3841 ggagagcatc agggatgaga tgaccaaatt catcccggcg cgataagaga tgatgagacc
     3901 gttaaataac ggagacgtca cccgttataa tactgaccgc gctcccgtca tctttcttga
     3961 taagcagatg tccctcgccg tcgatcccag tcgcgatgcc ttcgactaag tttccgtctt
     4021 ccttggcgac cctcacccgt ttattatatc cgccccacct ctcggtccat tccctgatta
     4081 tggatgtctt cccgcgcctg ttgaatgttt gataccacga ttcgatctcc agtagaaaat
     4141 ctcccgtaaa cttggccctg tcgatgtccg ctccgagact ctccttgagt gacgtcgcga
     4201 atcttgccgt ctcccccatc tcgcgtttga tctccgccct ggtcatgttt atattcacgc
     4261 cgacgccgac cacgatgtaa tccacccttt cccccctggg cgacatctcc gtgagcacgc
     4321 ccgccgcctt tttgccgtct atcagcacgt cgttcggcca ttttatcgaa gtattcctta
     4381 caccggtctt ctttatcgtt tcgacgagcg ctacggaagc tagaaaagtg aggagcgggg
     4441 attcgcacgc cgggatagac ggcctgaaaa gaatcgagat atagaggttc ataccgggcg
     4501 gcgagaacca tttccgtccg agcctgccct tgccgctcgt ctgcctgtcc gcgatgacgg
     4561 ccgtcccttc ggaagcgccc tgctcggcga gcctcgatag aacggtattg gtggaccccg
     4621 tctcgtcgaa gtagtgtatg accttgccga tcgtctctgt ctttaggaga ccgttcaatt
     4681 tgcgtatcat cggcgatgtc atgttaacta tcctccggcg cctttactga gcgtattcta
     4741 accgttttat cggctttgta gaatcctcac atattgtata acgcggagcc gctccgaacc
     4801 acgcaggata ccgggcggtg gaaaatcgag ggaatgtact tataatattc atggcggatg
     4861 tcttaccgga tgatatctat gtcgaatatt ctaaaaatat gcggtattgc ggccgcggcg
     4921 gtcgcgcttg ttctcccgct tcagtctctc tcgcgggccg agcagcagcc cgcggctata
     4981 gacagcccgg tgattgaggt caggataaag gacgtggtct caaaccccgg agaatacaac
     5041 gggaagaagg tctctctcga agggaaggtc gcgaaggtcg attacaggaa ttcgagcaaa
     5101 ggagagccgt tcacgatctt caagcttaag gactccgaga acaacgaagt cggggtatat
     5161 tttgaagatc agcgcctccc gctctcaaag ggagacagag tgaagatcat gggcaggttc
     5221 tggaaggaga agagttattt cctctataaa ataaagaacg tgataaaggc aaagaccgtc
     5281 gacatcatct agcaacctgc tgcagtgtcc ggcgttttac cgctgaccgg accctacccg
     5341 gatttaattt tcactttcaa acggacgatt cgtcatttaa tatctctttc atgaccctgc
     5401 tcgggaacgg gttaaagaga gtacttttat ttgtcatcct tttggtcgtt gtaatcctcg
     5461 ttgtcctcaa tatttatctg agcgggaagt taagacagct cgctcctgcg tacttagatc
     5521 gatactcgaa gctgagcggt cttactctcc gcgcggatga agcgggcctc gatccctttt
     5581 tcagaataag attaagcggc gtgaaggccg aaaacccgtc ccttcctcaa aatggactcg
     5641 ccgagatcaa gacgctgaca gtcgatcccg gaatcatctc ttcgctcttc aaccggagaa
     5701 tcacgata
//
LOCUS       DASSJH010000306         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1372651, whole genome shotgun sequence.
ACCESSION   DASSJH010000306 DASSJH010000000
VERSION     DASSJH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372651"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFJ67_06585"
     CDS             complement(<1..274)
                     /locus_tag="VFJ67_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290091.1"
                     /translation="MTNKAASISLYLVLPVIFMLGCSSTKYVHIEDPSERIVSGCISM
                     LPPTGGEWYVDKHWTEKLYGSDPCEDRLRLASGGGDDIYYIYITSRM"
     gene            complement(417..>2933)
                     /locus_tag="VFJ67_06590"
     CDS             complement(417..>2933)
                     /locus_tag="VFJ67_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7290092.1"
                     /translation="AFPSLSGEYVVETGGFLRNAAEAGDVAVGVRDGRPVYLRNVAAI
                     SDGPEEPSEYVFMGFGSASDKHRDGLSEAVTISFAKKKGTNATHIAERLHQKLDAMKG
                     SVIPEGVEIETTRNYGESAKEKSDELLKHMFIAALSVTFLIALALGWRESVVVLVAIP
                     VTLALTIFVTYMFGYTLNRVTLFALIFSIGILVDDAIVVVENIYRHFKMSGVSAPVAV
                     KAVDEVGNPTILATFTVIAALLPMAFVSGLMGPYMRPIPIGASAAMLISLFIAFIISP
                     WLSYIVLRGVKKSAGTEGGEGRLLKRLNHIYEGNLRGLLGSGKKRARAYGLVFLLLAA
                     SCLLVAFKAVTVKMLPFDNKSELQVIIDMPEGSTLEETAAVSREIGDYLGTVPEVLDY
                     ELYTGTSAPFNFNGLVRHYFLREGANVSDIQVNFVPRGERDTQSHDIAKRIRPRIKEI
                     GDKYGARIKIAEVPPGPPVLSTLVAEVYGPDPVRQREIARQVMNIFEGTEGVVDVDWY
                     VEDDQKKAVFEVDKEKAARNGISPDEIAETVRILLSGAPAGLAHVERDKEPVDIFLRA
                     SLPERAGVESLKDISLMTPEGGQVSVAELVNVRETLEDKTIYGKNLKRVVYVTADVAG
                     KKESPVYAILGMKDAIDELKLPEGYSLKQYSSTQPPMEDVYSMKWDGEWQITYEVFRD
                     LGIAFAAVLVLIYVMIVGWFRSFIIPFVIMAPIPLSLIGILPGHALMGAFFTATSMIG
                     FIAGAGIVVRNSIILVDFIELKLSEGMALKDAVVEAGVIRFRPMLLTASAVVVGSSVI
                     LFDPIFQGLAISLMAGEVAATLISRTMVPLLYYEYKSRVS"
BASE COUNT          623 a          879 c          771 g          660 t
ORIGIN      
        1 ccatgcggga ggtgatgtat atatagtata tatcatcccc ccctccgctc gcgagcctga
       61 gcctgtcttc gcacgggtcc gagccgtaga gcttctcggt ccagtgtttg tccacgtacc
      121 actcgccgcc cgttggcggg agcatggata tgcacccgct gactatccgc tccgacgggt
      181 cttctatatg cacgtatttt gtggacgagc agccgagcat gaaaatgacg ggcaatacga
      241 gatataacga gattgatgca gctttattgg tcattccgcg gtttctgaaa agccgttttc
      301 ggtctcacta tggaaaacca aaacgctcgt tagagacccc ccctccaacc caatataaag
      361 caatacgctt taccttgtca atagcgacgg ccgacaggcc tcatgacgca ggggcactaa
      421 gacactctgg atttatattc gtaataaagg agcggcacca tcgttctgga tatgagagtg
      481 gccgcgacct ccccggccat taaggagata gcgagaccct ggaagatggg atcgaagagg
      541 atcaccgacg accctacgac tacagccgat gcggtgagga gcatcggtct gaaccttatc
      601 acccccgcct cgaccaccgc gtccttgagg gccatgcctt cagaaagctt gagctcgatg
      661 aaatccacga gaattatcga attcctgaca actatgcccg cgcccgcgat gaagcctatc
      721 atcgacgttg cggtgaagaa cgcgcccatg agagcgtgcc cggggagtat gcctatgagt
      781 gagagcggta tgggagccat tatgacgaac gggattatga aagacctgaa ccatccgacg
      841 atcatcacgt atatgagcac gaggacggcc gcgaacgcta tgcccaggtc cctgaatacc
      901 tcgtatgtta tctgccactc cccgtcccat ttcatcgaat aaacgtcttc catagggggt
      961 tgggtcgagg agtactgttt gagagaatat ccttccggca gcttgagttc atcaatagcg
     1021 tccttcatcc cgagtatcgc gtagaccggg ctctccttct tcccggcgac gtcggccgtg
     1081 acatagacga cccttttcag gtttttaccg tatatggtct tgtcttcgag agtttccctg
     1141 acgtttacaa gctcagcgac gctcacctga ccgccttcgg gggtcatgag cgatatgtcc
     1201 ttcaggctct ctacgcctgc cctctcgggt aacgaagccc tcagaaagat gtcgaccggc
     1261 tctttgtccc tctcgacgtg agcgagaccg gccggggctc cggagagcag tatgcgaacc
     1321 gtctccgcta tctcatccgg gctaatgccg ttccgggcgg ccttctcctt atccacctcg
     1381 aacacggctt ttttctgatc gtcctcgacg taccagtcca catcgacgac gccttccgtc
     1441 ccttcgaata tattcatgac ctgccgggcg atctctctct gtctcaccgg gtcgggcccg
     1501 tatacctcgg ccacgagagt gctcaacaca ggagggccgg gaggcacctc ggctatcttt
     1561 atcctcgcgc cgtacttatc ccctatctcc tttatgcggg gtctgatcct cttggctatg
     1621 tcgtggctct gcgtgtccct ctctcccctg gggacgaagt tcacctgtat gtccgagaca
     1681 ttggctcctt ccctgagaaa gtaatgcctc acgaggccgt tgaagttgaa gggagccgag
     1741 gtgcccgtgt atagctcgta gtcgaggacc tcgggaactg tccccaggta atcccctatc
     1801 tcgcgggata cagcggccgt ctcctcgagt gtgctcccct ccggcatgtc tatgataact
     1861 tgaagctcgc tcttgttgtc aaagggaagc atctttacgg tgaccgcctt gaaagcaacc
     1921 aaaaggcacg aggccgcgag gaggagaaat acaaggccgt aggccctcgc ccttttcttg
     1981 ccgctcccta gaagccccct aaggttccct tcatagatat ggttgagcct tttcagcagc
     2041 cgtccttctc cgccttcggt cccggccgat tttttcacgc ccctgagcac tatgtagctg
     2101 agccaggggc tgattataaa cgctatgaac agcgaaatga gcatcgctgc ggaagcccct
     2161 atcggtatgg gcctcatgta cggacccatg agaccggaaa cgaaagccat cggaagaagc
     2221 gccgctatga ccgtaaaggt ggcgagtatt gtcgggttcc cgacctcgtc aaccgccttg
     2281 acagcaaccg gggcgctcac ccccgacatc ttgaaatgcc tgtagatgtt ctcgacgacc
     2341 actatagcgt cgtcgacgag aataccgata gagaatatga gggcgaagag cgtcactctg
     2401 ttgagcgtgt atccgaacat ataggtcacg aatatcgtga gagcgagagt cacgggtatc
     2461 gccacgagca cgactacgga ctccctccat ccgagggcga gggctatcag aaaggttacg
     2521 gaaagggcgg cgatgaacat atgcttgagg agctcgtccg atttctcttt cgcggactca
     2581 ccgtaattcc gggtcgtctc gatctcgaca ccctcaggta tgacgctacc tttcatcgca
     2641 tcgagctttt gatgcagcct ctccgctata tgagtcgcgt tcgtaccctt cttctttgcg
     2701 aaagagatag tgaccgcctc cgaaagccca tcccggtgct tgtcggaagc gctgccgaac
     2761 cccatgaaca catactcgga aggctcttct ggcccgtccg aaatcgcggc cacgttccgg
     2821 aggtatacgg ggcgcccgtc gcgcacgccc accgccacgt ctcccgcctc ggccgcgttc
     2881 cggagaaagc ccccggtctc gaccacgtat tctccgctca gtgaggggaa tgc
//
LOCUS       DASSJH010000307         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_400328, whole genome shotgun sequence.
ACCESSION   DASSJH010000307 DASSJH010000000
VERSION     DASSJH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400328"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /gene="gloA"
                     /locus_tag="VFJ67_06595"
     CDS             complement(<1..337)
                     /gene="gloA"
                     /locus_tag="VFJ67_06595"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015210883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HET7290093.1"
                     /translation="MRFLHTMIRVGDLEKSKKFYTEVLGLKFNRQKDYPEGEFTLAFL
                     GTDDQPEMLELTHNWGTDKYDLGNAYGHMAFGVDDIYAACEKIKSAGGKVVRPPGPMK
                     HGTTVIAFVE"
     gene            506..1441
                     /locus_tag="VFJ67_06600"
     CDS             506..1441
                     /locus_tag="VFJ67_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290094.1"
                     /translation="MMRLINISLCAVVIILFAGAAHAELYGVAHELEMQRAETSTPRI
                     NGIGESSLFLIDPDTGAATLIGPTGFYYCAGLDFHPVTNVLYAGCYNNIEGAGTEQVL
                     VTIDPQTGKGTEIGLFNANGILQDFSFSSDGTLYAYFRAKVSNFLATINIHTGAVNEI
                     GPSGLFGEGNGIGFDQFNDLFLTDTAMLPSLYLLNQSNGLATLVTDLTLPPLDEGFTV
                     INSLDLNPGTNVMFGSLDVQAFSYDNYLVTIDTGTGVVTNIGETVEGLEAIAFLSPRV
                     SNIPTMSEYGMAAIGLALLSISIFALYRRKRVAAD"
     gene            complement(1525..2568)
                     /locus_tag="VFJ67_06605"
     CDS             complement(1525..2568)
                     /locus_tag="VFJ67_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7290095.1"
                     /translation="MDENKKFKRPRLSRLLLALTAVLITIGAIEFAVRLTSRRDDIST
                     SENLVRPIFDGNPSGYRLRKTAWEASFTERGLEVPAGGPREGLRGEGVTPLKCAASDC
                     NFSKLVPGIIELNDDGVQSVGKGENLYPRILIVGGSVAWGAGASDISNTYFSKLYGML
                     KNDFPDTRISVLAYYGSTSNTDLISFVRKGLDVKPDIVVFINGLNDLTVKGQLRQTDA
                     SDYILNMKTAALIAGRNGIPIVIVRQPFPGGKKFKTDLEKRIMELSNKDYEKVITPLY
                     KHIGKVLEEMAKSGEIYYIDAADCFSNETATTFNDQWHFSDPGQEILAERIHAGLVPI
                     LSKILGKSGDNPD"
     gene            complement(2592..3038)
                     /locus_tag="VFJ67_06610"
     CDS             complement(2592..3038)
                     /locus_tag="VFJ67_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7290096.1"
                     /translation="MARIGRAFRELRVGDKLTERITVTETHVVRAAGLFNDYNALHTN
                     EPDMKNSRFGTRIVHGALTFSLVVGVYSKAFHDTDISTVEASIKFTAPVFINDTITME
                     WTVKELDEKPKLNGGLVVLSGEVRNMEEKVLATLEAKILVGNETIF"
     gene            complement(3126..>3522)
                     /gene="recR"
                     /locus_tag="VFJ67_06615"
     CDS             complement(3126..>3522)
                     /gene="recR"
                     /locus_tag="VFJ67_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET7290097.1"
                     /translation="PCEICRDEERDHSVICVVEEPLDQLAVERSREFTGVYHILHGVI
                     SPIEGVGPEDLRIKELLRRLEEGGVKEVIIATNPSVEGEATALYLSKLIKPLGIRVSR
                     IAHGIPMGGDIEYIDEITLSNAIRDRKTI"
BASE COUNT          756 a          901 c          874 g          991 t
ORIGIN      
        1 cttcgacgaa ggcgatgacg gtcgtgccgt gcttcatggg tccgggaggc ctcaccactt
       61 tccctcccgc ggatttgatc ttctcgcagg ccgcgtaaat gtcgtcgacg ccgaaggcca
      121 tgtggccgta ggcgttcccg aggtcgtatt tgtccgtgcc ccagttatgg gtgagctcga
      181 gcatctcggg ctggtcgtcc gtgccgagga atgcaagcgt gaattcgccc tccggatagt
      241 ctttctgcct gttgaattta agtccgagca cttcggtata aaatttcttc gatttttcga
      301 gatccccgac ccttatcatc gtgtgtaaaa aacgcatttt acacctccgt aatttctctc
      361 cggtactaat aaaagagaaa ctagcacagg ccatacttgg tgtcaaaagg tataagttta
      421 catatataac ttagtcttga tagtgaatta tcaaatgtgt taaaatgatg aaattagggt
      481 ttatttatta gaggaggatg actaaatgat gagactcata aatatttccc tgtgtgcggt
      541 ggtcattata ctctttgccg gcgcagccca tgcggagctc tatggtgtag cccatgagct
      601 tgagatgcaa agagctgaga cgagcacacc cagaatcaac ggcatcgggg aaagcagttt
      661 atttttaatc gatcccgata cgggtgcggc aaccctgata ggccccactg gtttttatta
      721 ttgtgcgggt ctcgattttc atccagtcac taacgtgctg tatgccggtt gttacaataa
      781 tattgaaggc gcgggcacgg agcaggtgct tgtaaccatt gatccgcaga ccgggaaagg
      841 taccgaaatt gggcttttta atgccaacgg tatcttgcag gacttttcat tcagcagtga
      901 tggtacatta tatgcgtatt tccgggcaaa agtcagcaat tttcttgcga ctatcaatat
      961 acataccgga gccgtgaatg agattggccc atcgggcctc ttcggtgagg ggaacgggat
     1021 cgggttcgat cagtttaacg atttattcct tacggatacg gctatgttgc cgtcacttta
     1081 ccttctgaat caatcgaacg gactcgcaac gttagtcaca gatctcacgc tgccgccgct
     1141 cgatgaaggg tttactgtaa ttaattccct cgatttgaac cctggaacga atgttatgtt
     1201 cggttcgctg gatgtgcagg cattttcgta tgacaattat ctggtcacga tcgacacggg
     1261 gacgggcgtg gtaacgaata tcggggaaac ggtagaaggc ctggaggcga tagcgtttct
     1321 cagtccgagg gtgtccaaca taccgacaat gtccgagtac ggcatggccg ccatcggtct
     1381 tgcattatta tcaatctcaa tctttgcgtt atatcgccgc aagagggttg cagcggatta
     1441 aaaaacgaac ataggaacaa cgtacccact acagctttca agggtgtgtg tgtcggtgtt
     1501 tgacccctta tccggtagct tggttcaatc tgggttgtct ccgctcttcc cgagtatttt
     1561 gcttaaaatc ggaacaagcc ctgcatgaat tctctcggcg agaatttctt gacccgggtc
     1621 cgagaagtgc cactggtcgt tgaaggtggt tgcggtctcg ttgctaaaac agtctgcggc
     1681 gtcgatataa taaatctctc ccgatttcgc catttcctcg agaacctttc caatgtgttt
     1741 atatagtggg gtgatgacct tctcgtaatc cttgttgcta agctccatta tcctcttttc
     1801 gaggtcagtc ttaaattttt tcccgcccgg gaacggctgt ctgacgatga ctatcggtat
     1861 cccgttacgc cccgctatca gggctgcggt tttcatattc agaatgtaat cggaggcatc
     1921 cgtttgtctc agctgtcctt tgacagtcaa gtcgtttaga ccgtttatga atacgactat
     1981 gtccggctta acatcgagtc ccttgcgtac aaaggagatt aaatctgtat tgcttgtcga
     2041 tccgtaatag gcaagtactg atattctcgt gtccggaaaa tcattcttaa gcatcccgta
     2101 cagttttgag aaatatgtgt tagaaatatc ggaggcgccc gccccccagg ctacgcttcc
     2161 gccgacgatc agtatgcggg ggtagaggtt ctcaccctta cccacggatt gtacgccgtc
     2221 gtcgttcagt tcaataatcc caggcacgag cttgctgaaa ttgcaatcgg atgcggcgca
     2281 tttcaagggg gtgactccct cgcctctcag tccttccctc ggccctccgg cgggcacctc
     2341 caaccctctt tccgtgaagc tggcctccca cgccgttttt ctgagcctgt agccgctggg
     2401 gttaccgtcg aatatcggcc tgacaaggtt ctcggatgtg gagatgtcgt ccctgcggct
     2461 agtcaatctg acggcaaatt ctattgcgcc gatcgttatc aggaccgcgg tcagagcgag
     2521 gagtaatctg gataagcgcg gccgcttgaa ttttttattc tcgtccattc atatatgagt
     2581 gtaattcccg cttagaagat cgtctcgttc ccaacgagta tcttcgcttc gagtgtcgcg
     2641 agcactttct cttccatgtt cctgacctct cccgaaagga cgacgaggcc gccgttgagc
     2701 ttgggctttt cgtcgagctc ctttaccgtc cactccatgg ttatcgtgtc gtttatgaag
     2761 acgggcgccg tgaactttat agaggcctcg acggtcgaga tgtccgtatc gtggaacgct
     2821 ttgctgtaaa cgcccaccac gaggctgaac gtcagagccc cgtggacgat cctcgtcccg
     2881 aacctcgagt ttttcatgtc tggctcgttc gtgtggagcg cgttgtagtc gttaaagagc
     2941 cccgccgccc tgaccacgtg ggtttccgtg actgtgatcc tttccgtgag cttgtccccg
     3001 accctgagct ccctgaacgc ccttcctatc ctcgccatcg catcgtcctc cggaaaatgt
     3061 attatttata tccgggatat atattatatt agatatataa tagacatcat ggaaatgaaa
     3121 atatcctata tagtttttct atcacgtatt gcattactta atgtgatttc gtcaatatat
     3181 tctatatccc cacccatggg tatcccgtgt gcgatcctgc tcactcttat gcccaggggt
     3241 tttatcagct tcgagagata gagcgccgtc gcctcgcctt cgacgctggg gttggtcgcg
     3301 attatgacct cctttacgcc gccctcttcg agcctgcgga ggagctcctt aatcctgaga
     3361 tcttcgggcc cgaccccctc tatcggggat atgacaccgt gcagtatgtg atatacgccc
     3421 gtgaattccc tgctcctctc tacggcgagc tggtcgagtg gctcttccac gacgcagatg
     3481 acggaatggt ctctctcctc gtccctgcat atctcgcagg gc
//
LOCUS       DASSJH010000308         5951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1373612, whole genome shotgun sequence.
ACCESSION   DASSJH010000308 DASSJH010000000
VERSION     DASSJH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373612"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3546
                     /locus_tag="VFJ67_06620"
     CDS             <1..3546
                     /locus_tag="VFJ67_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290098.1"
                     /translation="VTLARNVGFMSIGHGFYLEDGTEINNKLYSNIGILVRAAVKNPQ
                     NPRQVPGILAWSGNPNTAQVPYNSDVFNPTVFWIMNGWNDFEYNMAAGAAGCGVCYWL
                     VPGTNSGPSQEMRWESYASMQTGPVITQGSKVYQQNIDRAGMTPLMTFKGNYCTSAMM
                     SFNTVGITDACLGVSNFGAATNPNAPAPEGDINMETFYPKVDGGGGRFGTRCDGTDCS
                     RTAIPNRCSVGDRSRCTVTVLDGYTSSFHWPQQNFAAIWLRPQWYLVINSFLSDVQNG
                     GLGFVTGGDYLLSSVIPGLWQLATKSVFVGHTQTDNPLASDAGPFNPLKSPDMKISGL
                     ICDNQDANHCRSAKEGISIPLDNFAVNQRFFNIYDGPNYQDSNAYLDITETNLSPICQ
                     PGGASYCPQLGPGAGMAAWTYTRVPGIPVNKTKNACVLPNAAIGWKQPNGFFYPPGFH
                     SEKLYFHNVDIRHYVLEPLWDAGTYTTNEDKVKTDYCTFPASNMFGNFSSVDRQTVLN
                     DDDGSLTGLLSPPVPAKGETISVNLDPFFNAPIEAGECESFSTAKTSPYEYVSTVVYP
                     GCVADKDHGYCGGTCMIDKKACLDNSNCIGACKIDHQPCTTNNDCNMSNPDNTCDFGI
                     NGCDLTTSYWGRPCTNATCFGIPLERQYLTSADKADLSTTGIRMAGMDLFQRSMLTLN
                     NAKYYIDTTVSETEQRKSAQSLNVFKAGQTYYVFFLYGKANTKQTYEFYVGDGLKLGD
                     ATDAMDVKIKRANIAGFPITFTDGPTDWKSVGWDKTYKDGILTVTVDMSSFATEFADT
                     KKDFCQPASFCTLTGDTCGCSSSLLTDQLAACQAGNICGKFAGKDIDCPKNGCLGFSF
                     KLPNPGFDTDNKGNIVQSKGGHRPDPTCFPNEAPWNISLTRPDAEVAGSCSNTPIDGP
                     KFCTNLGGPPPTTEPTPVPTPVPTATPPPPPSNDKDNDGVPDDVDADADNDGIPNSME
                     SGSGDTNSLASARLVISNDPDGDGIPSEHDLDSDGDGLPDHFEGGGHNDSNSDGTADG
                     FADTDGDGLHDAHDPDQGGNVLPLPDTDGDGLPDFLDTDSDNDGITDTNETAGCVDGN
                     GDGELDDSEDTNGDGLPDSVHPATGAPCGLIDSDGDGIFDHLDATDDGGGQEEEGGGN
                     CAIAGGGSGKGGLAGMLLIYSLIPMGILIRRRKRS"
     gene            complement(3697..4866)
                     /locus_tag="VFJ67_06625"
     CDS             complement(3697..4866)
                     /locus_tag="VFJ67_06625"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET7290099.1"
                     /translation="MSVIQNRCFERRETILKAAIWFMSFLAVSRLAYQLWRLLLNPGW
                     NGAIDLRIMYESVNQWFKGEALRELIHLPATYAIIWPLTGWLSFESARWLWAVLYAVS
                     LVWLFRIVAAETGIKKREEILFAALFILSIYPTGITIGNGQVILFLLPCILTAVFMER
                     EHESGIIRDIILSLLFLISSLKITVTAPFFLIALITQRAIRPLTIAACGYLILTYVAI
                     SYRDAGLVETLKIWMHDCSAVAARGGYANIHIWLITLGLERLIFPASFVLIAAFSLWL
                     FKFRKTDIWIQLGVAAIVARIWTYHRLYDDLLILVPIIAIFRIVRNNQLSPNERLAAD
                     LLLVITWLALLSPGTFLQLPPPLGTLFRTGQAVIWLSLLVFLVYYSYKAVRTGPA"
     gene            5109..>5951
                     /locus_tag="VFJ67_06630"
     CDS             5109..>5951
                     /locus_tag="VFJ67_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7290100.1"
                     /translation="MKVGIIGAGPAGLACAYKLSKAGVDVDVFEAGGSVGGLARSFRL
                     WNQTVDMGPHRFFSRDPRVNGLWLEVVGRDYSMVNRLTRILYKNKFYQYPLRPADVVG
                     KLGVLETSRALLSYVREKIAPETSRTGEETFESWVVRRFGRRLYEIFFKTYSEKLWGI
                     PCGELDADFAVQRIKKFSLYEALKTAFANDRSGKHKTLVDRFAYPFLGTGMVYERRAA
                     HVSRNGGGVYMNTPVRRVVSAGMRVTGIEFPDGRTEAYDHIVSTMPLTLLVLGLDGVS
                     AEVTA"
BASE COUNT         1498 a         1506 c         1720 g         1227 t
ORIGIN      
        1 gtaacgctcg caaggaacgt gggattcatg tcgataggtc acggttttta cctcgaggac
       61 ggcacggaga taaacaataa gctctattca aatatcggca tattggtccg tgcggcagtc
      121 aagaaccctc agaacccgcg ccaggtcccg ggaatcctgg catggagcgg aaaccccaat
      181 accgcacagg tcccctataa ttcggacgtg tttaatccga ctgtattctg gatcatgaac
      241 ggatggaacg atttcgaata caacatggca gctggcgcgg ccgggtgcgg ggtgtgctac
      301 tggctcgtcc cgggcacaaa cagcggtcct tctcaagaga tgagatggga gtcttacgcc
      361 tctatgcaga caggcccggt tataacccaa ggaagcaagg tttatcagca gaatattgac
      421 cgcgcgggta tgacccctct catgacgttc aagggcaatt actgcacatc cgccatgatg
      481 tcgtttaata cggtcggtat taccgacgca tgtctgggcg taagcaactt cggagcagcc
      541 acgaacccga acgctcccgc gccagagggc gacatcaaca tggagacgtt ctatcccaag
      601 gtcgacggcg gcggcgggcg gttcggcacg cgctgtgacg ggaccgattg cagccggacc
      661 gcaattccca acaggtgcag cgttggagac aggagcaggt gtacggttac tgtactcgac
      721 ggctacacgt catccttcca ctggccgcag cagaatttcg cagcaatatg gctgcgcccg
      781 caatggtatc tggtgataaa cagtttcctg tccgatgtac agaacggagg gttaggcttc
      841 gttacaggcg gggactattt gctctcaagc gttataccgg ggctatggca gctggcgacg
      901 aagtcagtgt ttgtaggaca cacacagacc gataacccgc tcgcgtcgga cgcaggaccg
      961 tttaacccgc tcaaaagccc tgatatgaag atttccggtc ttatctgtga taaccaggac
     1021 gcaaaccact gcaggtcggc aaaggaaggc ataagcattc cgctcgacaa ctttgcggtc
     1081 aatcagaggt tcttcaacat atacgacggg cccaattacc aggattcgaa cgcttacctc
     1141 gacataacgg agactaacct cagtccaata tgccagcccg ggggggcgag ctattgcccg
     1201 cagctcggac ccggagcggg catggccgca tggacatata ccagggttcc aggcattcct
     1261 gtaaacaaga ccaagaacgc atgcgtcctt ccgaacgcgg caatagggtg gaaacagccg
     1321 aacgggtttt tctatccccc ggggttccat tcggagaagc tttatttcca caatgtggat
     1381 atacgacatt atgtgcttga gccactttgg gatgccggta catatacaac gaatgaggat
     1441 aaggtcaaaa cggattactg cacgtttccc gcaagcaata tgttcgggaa cttcagcagc
     1501 gtcgacaggc agaccgtgct gaacgacgac gacgggtcgc tcaccggact attgagtccc
     1561 cctgttcccg ccaagggtga aactatttca gtgaacctcg accccttctt taacgctcct
     1621 atagaagcgg gggaatgcga gtcgttcagc acggcgaaga caagtcccta tgagtatgtc
     1681 tccacggtgg tgtatccggg ctgcgtggcg gataaggatc atggttactg cggcggcacg
     1741 tgtatgatcg acaagaaggc atgcctcgat aacagcaatt gcatcggagc gtgcaaaatc
     1801 gatcaccaac cttgtactac caacaacgac tgtaatatga gtaatccgga caatacgtgt
     1861 gatttcggca ttaacgggtg cgatctcacc acttcatact gggggaggcc gtgtacgaat
     1921 gccacatgct tcggcatccc gctcgagagg cagtatttga cctcggcgga taaggcggac
     1981 ctgtccacta cggggatacg catggcgggt atggacctgt tccagcggag catgctgacg
     2041 ctcaacaacg ccaaatacta catagacacg accgtatccg aaacggagca gaggaagagc
     2101 gcgcagagcc tgaacgtctt taaggcaggc cagacttatt acgtattctt cctctacggg
     2161 aaggccaata ccaaacagac ctacgagttc tatgtaggcg acgggctgaa actcggcgat
     2221 gcaacggatg cgatggatgt aaagataaaa cgggctaaca ttgccggttt tccgataacg
     2281 tttacagacg gacccactga ctggaaaagc gttgggtggg ataagacata caaggacggc
     2341 atattgactg taacagtcga tatgagctcg ttcgctacgg aattcgcgga cacaaagaaa
     2401 gatttctgcc agccggcgag cttctgcacg ttgaccgggg atacgtgcgg atgctcgagc
     2461 tcgttgctca ctgaccagct tgcggcttgt caggcgggca atatatgcgg caaattcgcg
     2521 gggaaggata tagattgccc caagaacggg tgcctgggat tttcgttcaa gcttccgaac
     2581 cccgggttcg acaccgacaa caagggaaac atcgtgcaaa gtaagggcgg gcacagacct
     2641 gatccgacat gcttcccgaa cgaagctcca tggaatatca gcctgacgcg cccggatgcg
     2701 gaagtcgcgg gatcgtgctc caatacgccg atagacggtc ccaagttctg caccaacttg
     2761 ggaggtccgc cacccacgac tgagcctacc cctgtgccca cgcctgtacc tacggcgaca
     2821 ccaccacctc ctccgtcaaa cgacaaggac aacgacggtg tgccggacga cgtggatgcg
     2881 gacgccgata acgacgggat tccgaacagt atggaatcgg ggtcgggaga tacgaactca
     2941 ttggcctcgg cgcggcttgt aatatctaat gatccggacg gggacggtat cccgagcgag
     3001 cacgatctgg attcggacgg ggacggtctg ccggaccatt tcgaaggcgg cggtcataac
     3061 gattcgaaca gcgacggtac cgccgacggc ttcgctgaca cggacgggga cggtctccac
     3121 gacgcgcatg atcccgatca gggcggtaac gtcctgccgc tacccgatac ggacggtgac
     3181 ggattgcctg acttcctgga cacggactcc gacaacgacg gcataaccga tacgaatgag
     3241 actgcgggct gtgtggacgg gaacggggac ggggagctcg atgactctga agatacaaac
     3301 ggtgacggct tgcccgacag cgttcacccc gcgaccggag cgccctgcgg attaattgat
     3361 tccgacggtg acggtatatt cgatcacctg gacgctaccg atgacggcgg cgggcaggaa
     3421 gaggaaggcg gcggcaactg cgccatagcg ggcggcggaa gcggaaaggg cggactggcc
     3481 ggaatgctgc ttatctactc cctgatacct atgggcatcc tgatacggag gaggaaaagg
     3541 agttaacaga ccgtcgttac gaccctcggg aaatacattg atgaaattga aagtcttctg
     3601 gcggaaacgg ccggcagcta tcttcggtat atattacaaa cgtatcttaa attgaatcaa
     3661 ggcatggagg gttattgtcc ggttcggaaa tcttattcaa gcgggcccgg ttcgtactgc
     3721 cttatatgaa tagtagacga ggaacactag cagggataac cagataactg cctggccggt
     3781 tctgaagagt gttccgagag gtggcgggag ctggaggaat gtcccgggag agagaagggc
     3841 cagccaggtg atcacgagca ggaggtcggc tgcgagcctt tcattggggg aaagttgatt
     3901 attcctgaca atcctgaaga ttgcgattat cggaacgagg attagtaaat cgtcgtataa
     3961 acggtgatac gtccaaatgc gggctactat tgccgcgacc ccgagctgaa tccagatgtc
     4021 tgttttacgg aatttgaata accagagtga gaacgctgcg atgaggacga acgatgcagg
     4081 gaatatcaat cgttcgaggc ccagggtaat gagccagatg tgaatattcg catatccccc
     4141 ccgtgccgcg acggcggaac aatcatgcat ccagatttta agtgtctcaa caaggcccgc
     4201 gtctctgtat gatattgcaa cataggtcag tattaaataa ccgcaagcgg caatggttaa
     4261 agggcggatc gctctttgag taatgagcgc tatcagaaag aacggcgctg taacggtgat
     4321 cttgagagag gaaataagga aaagcaaaga gagtattatg tctctgatta tcccgctttc
     4381 atgctccctt tccataaaca cggccgtcaa gatacacgga aggagaaaga ggataacctg
     4441 tccgttgccg atcgttattc cggtcgggta gatcgataga ataaatagag cggcgaacaa
     4501 tatctcttcc cttttcttga tcccggtttc tgccgccact atccgaaaga gccacacgag
     4561 cgagacagca tataaaacag cccagagcca tctcgccgat tcgaaggaga gccatcccgt
     4621 gagcggccag attatcgcgt aggttgcggg aaggtggatg agctccctga gcgcctctcc
     4681 tttaaaccat tggttgacgc tttcatacat gatccggaga tcgatcgcac cgttccaacc
     4741 cggattaagc agaagacgcc atagctgata agcgagcctc gagacagcga ggaacgacat
     4801 gaaccagatc gcagctttta atatggtttc ccgccgctcg aagcagcggt tttggattac
     4861 ggacaatcga tcgattagta tgaagcggct cctgttcacg ctgtccggtg tacggtatcc
     4921 gtgacacctc gaccgatata ataacctttc tccttgtttt ttagaatgag gggtgggact
     4981 ctatttacgc tttctttttc agaacagcct tttttgcgga ctccctttca atttttttaa
     5041 gaaattatat tatctatgct gatacagaca accgtaaatt gcaggaaatc ggtcggggac
     5101 aatcacgaat gaaagtcgga ataatagggg ccggtccggc gggactcgcg tgcgcttaca
     5161 agctctcgaa agcgggagta gacgtggacg tgttcgaggc gggcggttct gtaggcggac
     5221 tggccaggtc gttcagactg tggaaccaaa ctgtggacat gggcccgcac cgattcttca
     5281 gccgggaccc ccgcgtcaac ggactatggc tcgaggtcgt cgggcgtgat tattcgatgg
     5341 tcaaccggtt gacgagaata ctttacaaaa acaagttcta tcagtacccc ctgcgaccgg
     5401 ccgacgtggt cgggaagctg ggcgtgttgg agacatcacg cgcattactg agttacgtcc
     5461 gcgagaaaat agcgcctgaa acgtcccgta cgggggaaga gaccttcgag agctgggtcg
     5521 tcaggagatt cgggaggagg ctctacgaga tattcttcaa aacctacagc gaaaaactgt
     5581 ggggcattcc atgcggagag ctcgacgcag actttgcggt gcagaggatc aagaagttct
     5641 cactgtacga ggctctaaag acggcgttcg cgaacgacag aagcggaaaa cacaagacgc
     5701 tcgtagatag gtttgcctac ccttttctgg gtacggggat ggtttatgaa cggagggcgg
     5761 ctcatgtttc ccggaacggc ggcggggtgt atatgaacac gcctgtcaga agggtcgtaa
     5821 gcgccgggat gagggtaaca ggcatagagt ttccggacgg aaggaccgag gcttacgacc
     5881 acatcgtatc gacgatgccg ctgacgctgc tggtcctcgg gctcgacggt gtgagcgcgg
     5941 aggtaacagc a
//
LOCUS       DASSJH010000309         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_292079, whole genome shotgun sequence.
ACCESSION   DASSJH010000309 DASSJH010000000
VERSION     DASSJH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292079"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /gene="carB"
                     /locus_tag="VFJ67_06635"
     CDS             complement(<1..1725)
                     /gene="carB"
                     /locus_tag="VFJ67_06635"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7290101.1"
                     /translation="MPKRKDIKTILLVGSGPIVIGQACEFDYSGTQACKALKEEGFRI
                     VLVNSNPATIMTDPAFADRTYIEPLVPAIVEKIIEKERPDALLPTLGGQTALNIAVDL
                     AESGVLERYGVELIGAKLHAIKKAENRELFKEAMLKIGLEVPRSGVAHAMDEAWNVMR
                     DLGFPVIIRPSFTLGGSGGSVAYNKEEFEEFAKSGLDLSPVSEILIEESVLGWKEYEL
                     EVMRDGMDNVVIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQRMRDASIAIIREIG
                     VDTGGSNIQFGLNPEDGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     ISNDITRYTPASFEPSIDYVVVKIPRFTFEKFPQTDPTLTTQMKSVGEVMAIGRTFKE
                     SLQKAMRSLEIDSSGFEPRSADTGLISDKLRTPNSERLWYIADAFRCGMDVEDIFSLT
                     YIDRWFLRNIEQIVRLEDELKSRSRKMNGSLLKRAKEFGFSDIRIAELTGKSQDDVRK
                     SRVRSKIASVYKMVDTCAAEFEAYTPYLYSTYEREDEAPPTDRSKVVILGGGPNRIGQ
                     GIEFDYCCV"
     gene            complement(1969..2694)
                     /locus_tag="VFJ67_06640"
     CDS             complement(1969..2694)
                     /locus_tag="VFJ67_06640"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7290102.1"
                     /translation="MMTQPEEITERSKNKRRPESTTLLGSGIKTWWLLLMLPIEDYLL
                     KIRVHPNVLTVSTLVVAIITGLFYHYGWIFWGGILVLAGSTFDIFDGRVARAQGLSSE
                     HGAFFDSCLDRFSEAFIYLGLLSLFRGTIFSYVVFLILVSTTMVSYTRARAEGLGVKC
                     EVGVMQRAERVVYLGVLSIFNFLGNFIAEALGFGPDDYLLKLALLLMLVFSSYTAVQR
                     MFHVMTELRKREEQKKIEPGTVP"
     gene            complement(2819..>3588)
                     /locus_tag="VFJ67_06645"
     CDS             complement(2819..>3588)
                     /locus_tag="VFJ67_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7290103.1"
                     /translation="IKAEDNLPLTERPAETPAQVSIPGGSNEEIRLVLASQMESSASL
                     DGSIIKAPAAAETSAPEPAGNNVPAVTETRPPETARDTVPDVSSNETVSDGETLKEAP
                     IESLSEEPRKQDSRPPATTANIPENTQANSSDDSTAEMIEGQLLASFTGGAQEDAVPD
                     AADPGIRQEPRPKNGRGFTVQVASYRDSKDAEELAQRLARKGYQAFVAEADIPGKGKW
                     YRVRVGRFGTRKEAASFGENLKRKERSLKSVYVAEDD"
BASE COUNT          732 a         1048 c          953 g          855 t
ORIGIN      
        1 gacgcagcag taatcgaact ctatgccctg cccgatcctg ttcggtcccc cgcccaggat
       61 cactactttg gacctgtcgg tcggcggggc ctcgtcctct ctctcgtatg tcgagtaaag
      121 gtaaggcgta taagcctcga actcggcggc gcacgtatcg accattttat acaccgaggc
      181 gatcttgctc cggactctcg atttcctcac atcgtcctgg gattttcccg tcagctcggc
      241 gatcctgata tcggagaagc cgaactcctt ggccctcttg agaaggctgc cgttcatctt
      301 gcgagatcgg gacttgagct catcctccaa gcgaacgatc tgctcaatgt tcctgaggaa
      361 ccacctgtct atgtacgtga gactgaaaat atcctctaca tccattccgc acctgaaggc
      421 atcggcgatg taccatagcc tctccgaatt aggcgtcctc agcttgtcgc ttatgagtcc
      481 ggtatcggcc gagcggggct cgaaccccga ggaatcgatc tcgagcgacc tcatagcttt
      541 ttgcagcgat tctttaaacg tcctccctat cgccatgacc tcgcctacgg atttcatctg
      601 cgttgtgagc gtcgggtccg tctgcgggaa tttttcgaaa gtgaacctcg gtatcttgac
      661 cacgacataa tctatagagg gctcgaaaga agccggcgtg tagcgcgtga tgtcgttcga
      721 tatctcgtcg agcgtatagc cgaccgcgag ctttgccgcg atcttcgcta tgggaaagcc
      781 ggtcgccttc gacgcgagcg cagagctcct cgagaccctg gggttcattt ctattaccac
      841 catgcgcccg tcctcggggt tgaggccgaa ctggatgttc gagcctcctg tatcgacgcc
      901 gatctccctg attatcgcta tagaggcgtc gcgcatcctc tgatattcct tatccgtgag
      961 cgtctgggcg ggggctacgg ttatgctatc cccggtatgc acacccatgg ggtcgaaatt
     1021 ctcgatcgag cagattatca cgacgttgtc catgccgtcg cgcattacct cgagctcgta
     1081 ctccttccac ccgagcacag attcttctat cagtatctcg ctcacggggc tgagatcgag
     1141 tcccgacttg gcgaactcct cgaattcctc cttgttatag gcgacgctcc ccccggagcc
     1201 gccaagagtg aacgacgggc gtattatgac cgggaatccg agatctctca ttacattcca
     1261 ggcctcgtcc atagcatggg ccactccgct cctgggcact tcgagcccga tcttgagcat
     1321 ggcttccttg aagagctccc tgttctccgc ctttttgatc gcgtgcagct tggcccctat
     1381 aagctcgacc ccgtacctct ccagcacgcc agactcggcc agatcgaccg caatattgag
     1441 ggcggtctgt cccccgagag tcgggagcag ggcgtcgggc ctctcctttt ctattatctt
     1501 ctctacaata gcgggcacga ggggctctat gtaggtcctg tcggcgaacg ccgggtcggt
     1561 catgatcgtg gccgggttcg agttaacgag cactattctg aagccctctt ccttgagggc
     1621 cttgcaagcc tgggtgcccg agtagtcgaa ctcgcaggcc tgacctatca ctatcgggcc
     1681 cgacccaacg agcagaatcg ttttaatatc tttccgttta ggcatataag tattgcggag
     1741 tctgagtcaa ttttcgaaaa acgagaatat cgggacttgg gtaaaaacgc ctgagccccc
     1801 gacagcgaag cacttgattt atacctgtga atttgccgtc tagcaaatgg cggtatccgc
     1861 gtaacagcgt gataaaaacg gggcatccgt acccgatatt aaggggattc ggccggcatt
     1921 ttcgggaccc gaatagatcc tggtaaggac aagcggaact gggaagaact agggaactgt
     1981 ccccggttcg atcttctttt gctcctccct cttcctgagc tcggtcataa cgtggaacat
     2041 gcgctggacc gcggtgtaag aagagaaaac cagcatcagt aaaagcgcca gcttgaggag
     2101 ataatcgtcg ggtccgaagc ccagcgcctc tgcgataaaa ttgccgagga agttaaagat
     2161 cgagagcact cccaggtaaa cgaccctctc ggccctctgc atgacgccga cttcgcactt
     2221 cacgccgagc ccctccgccc tcgcgcgcgt gtagctcacc attgtagtcg aaacgagaat
     2281 gaggaatacg acgtatgaaa aaatcgtgcc cctgaataag ctcagcagtc ccagatagat
     2341 gaaggcctct gagaacctgt cgaggcagga atcgaaaaag gcgccgtgct cgctgcttaa
     2401 gccctgagcc cttgcgacac gtccatcaaa tatgtcgaac gtcgatcccg cgagcacaag
     2461 aatcccgccc cagaatatcc agccgtagtg ataaaagagg cccgtgatta tggcgacgac
     2521 gagcgtggaa acggtgagca cgttcggatg taccctgatc ttgaggagat aatcctctat
     2581 cggcagcatc agcaacagcc accaggtctt gataccgctt ccgagaaggg tcgtggactc
     2641 gggccttctc ttgtttttag acctttcggt aatctcttcg ggttgagtca tcatgctccg
     2701 ttccagaatt aaaataatac ggcaattccg ggaataacaa tatccgtttt ggctttttca
     2761 ggcaaggcgg ggagaagtag cggggaatga cgcttctaaa acatcagaaa acctggaatc
     2821 aatcgtcctc ggcgacgtag acggacttga gagaacgctc ctttctcttt aagttttctc
     2881 cgaacgaggc ggcttccttc ctcgttccga accggcccac cctgaccctg taccacttgc
     2941 ccttcccggg aatatccgcc tcggctacga aagcctggta gcccttcctg gcgagtcttt
     3001 gcgccagctc ttctgcatct ttgctgtccc tgtaggacgc tacctgcacc gtaaaccctc
     3061 tcccgttctt cggccggggc tcttgcctga tgccgggatc agcggcatct ggaacagcgt
     3121 cctcctgcgc ccctcccgta aaggaagcaa ggagctggcc ttcgatcatc tcagctgtag
     3181 agtcatccga gctgtttgcc tgggtgttct cgggtatgtt cgcggtagtg gcgggcgggc
     3241 gggagtcctg cttccggggt tcttcagaga gcgattcgat cggagcctct tttaatgttt
     3301 caccatcgct cacggtttca ttactactta cgtccggcac tgtatccctt gccgtctcgg
     3361 gcggacgagt ctcggtaacg gccggtacgt tattcccggc aggctcgggc gcgctggtct
     3421 cggccgcggc gggcgctttg attatggatc cgtcgagcga ggctgaagat tccatttggc
     3481 tagcgagtac gagccttatc tcttcattac tgccgccggg tatcgagacc tgtgcaggcg
     3541 tctccgcggg gcgctccgtc aggggaagat tatcttccgc tttaatgc
//
LOCUS       DASSJH010000310         4737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1373703, whole genome shotgun sequence.
ACCESSION   DASSJH010000310 DASSJH010000000
VERSION     DASSJH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373703"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /gene="hisD"
                     /locus_tag="VFJ67_06650"
     CDS             <1..1270
                     /gene="hisD"
                     /locus_tag="VFJ67_06650"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET7290104.1"
                     /translation="KLRRGSSLSDPALETSVKKILEDVRKNGDRALFKYTKRFDRVVL
                     TAKTVRVSKTEFRKARSAVPKELLDDLALAGKRIRDFHRLKLPSGNTFRDTLGNELGW
                     VIRPIEKAGLYVPGGKAAYPSTVLMTAIPAKVAGVKELSLVTPCPEGEVNPAVLAAAE
                     IGGVDSVYKIGGAQAVAALAYGTESVPKVDKIVGPGNIYVTIAKKLVFGEVDIDMIAG
                     PSEVLIVADGSAPAEWVAADLLAQAEHDEMAVPAVVTDSASYAAEVSKEVSSQLLRLE
                     RKAIAGESVRKQGRIFVTRNIEQAIDLANAFAPEHLELCVAEPRSILKKINNAGAIFL
                     GSMSTEAFGDYIAGPSHVLPTGGAARFSSPLSVYDFLRMPSVISVSRKGFDTLSDSVM
                     NLAYSEGLEAHALSVSVRTDVAALIRKPEN"
     gene            1300..2403
                     /gene="hisC"
                     /locus_tag="VFJ67_06655"
     CDS             1300..2403
                     /gene="hisC"
                     /locus_tag="VFJ67_06655"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET7290105.1"
                     /translation="MPDKSKEKGKSKARGLTESRVREEIRELSAYSVPHIEAPVKLDG
                     NESPFSLPPEVGEKVIEKLRSVDVNRYPDPEALGLRELISRINDFPLGGILLGNGSDE
                     LIGMLIAACSGGTGRVLCPAPTFSMYRITGVALGREVLEAGLDRNFDLDMEAMPALID
                     EKDPDLIFLASPNNPTGNAYSSDRIRELIKLSKGVVVVDEAYSDFSGHTFLPLINKHE
                     NLVILRTLSKVGFAGLRLGILYGREELVREINKVRYPYNINSLTQAAAGVVLGNHEFV
                     TENIQLIVRERDRVYKALSGMKGVEAFRSDANFILFRVGDADSVFKALVERGVLIRNF
                     NRPGRLENCMRVTIGTPYENDSFLNALGSALSS"
     gene            complement(2365..3942)
                     /gene="lnt"
                     /locus_tag="VFJ67_06660"
     CDS             complement(2365..3942)
                     /gene="lnt"
                     /locus_tag="VFJ67_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET7290106.1"
                     /translation="MKRISGIDLVLAALTGVLYPLTFMIPHTGILAWVLLIPLFWAID
                     NKSAKDAFKLGLLAGLVSNFAGTYWLIGTLSRFGGFPILVSVLFIAVLSAFSGLSYAI
                     FSYIVTRLGFLRRPGVVSALLVAAVWTSIEYFFPFLFPYGIANSQADYIPVIQIFDLF
                     GVYALSFIIVVVNLALMRVLKNLTGESRFPAGEIVLALALVISTLVYGYIRIGTVDGE
                     IARAPKLKIGMVQANFDFLEKSESQEEVVTKRHKEMSIDLKSDDLIIWPETAIQAWFP
                     IESDYLLVREEMGVPKMDGKYFIVGGMSFTPKNPNGEVKSDDDLLKYNTAFLANSDGV
                     IMGRYHKIKLLLFGEYLPFTNLIPALKSISPASGDFTPGRELNLFEIEDKGARIAPII
                     CYEDIIPSFSRRFVEKGANLIVNITNDAWFGRTVAPYQHMFVSIPRSVETRRYLLRST
                     NTGISAVIDPVGRVVAKTPIFVRTNLESEVGLMNGGLTLYTKLGDVFPIACLVFWAGY
                     AVIVWLRGKSASERVEE"
     gene            complement(4024..>4737)
                     /locus_tag="VFJ67_06665"
     CDS             complement(4024..>4737)
                     /locus_tag="VFJ67_06665"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7290107.1"
                     /translation="ITLEAKRKGLNEKLVGNLFLATMAGGIIGAKLLYVFENVPLSDI
                     VSHPLENLLSRGGLTFYGGFFGAIAIVWFIALRNKLSMWVVCDLAAPALALAYAIGRI
                     GCFLVGDDYGVPSNLPWAIPFPKGLPPTTVPVHPTQVYEIIIMSIVFLFIWKIRKLER
                     PAGWLFSIYLILAGLERFFIEFLRSTTPSPIPGLSVAQVMAVVIIIVGAVKLYRLHSA
                     GETELAPASPKQPVKKKKA"
BASE COUNT         1099 a         1327 c         1346 g          965 t
ORIGIN      
        1 gaagctcagg agggggtcct cgctttcaga ccccgcgctc gaaacctccg tgaagaaaat
       61 tctggaggac gtcaggaaga acggggacag ggctcttttc aaatatacga agaggttcga
      121 cagggtcgtg cttacggcca agaccgtcag ggtttcgaag accgagttca ggaaggcgcg
      181 ctcggcggtg cctaaggaat tgctcgacga cctagctctc gccggaaagc gcataaggga
      241 ttttcacagg ctcaagcttc cctcgggaaa cacgttcagg gatacgctcg gcaacgagct
      301 cggctgggta ataaggccca tagaaaaggc ggggctatac gtgccggggg ggaaggccgc
      361 gtacccttcg acagtcctca tgaccgcaat accggcgaag gtggcgggag tgaaggagct
      421 ctcactggtg acgccttgtc ccgagggcga ggtaaacccg gcagtgcttg cggcagccga
      481 gattgggggt gttgactccg tctataagat aggcggcgct caggcggtag cggcgctcgc
      541 ttacgggact gaatctgtcc cgaaagtcga caaaatagtc ggtcccggaa acatatacgt
      601 caccatagcg aagaagctcg tcttcggaga agtcgatata gatatgatag ccgggccgag
      661 cgaggtgctc attgtcgccg acggcagcgc cccagcagaa tgggtggcgg ccgatctcct
      721 cgcacaggcg gagcacgatg aaatggcagt ccctgccgtc gttacggact cggcctccta
      781 cgcggccgag gtctcaaaag aggtttcgag tcagcttctc aggctcgaga ggaaggcgat
      841 cgcaggcgaa tccgtaagga aacagggcag gatcttcgtc acacggaaca tagagcaggc
      901 gatagattta gccaacgcct ttgctccgga gcacctcgag ctctgtgtgg ccgagccgag
      961 atccatcctc aagaaaataa acaacgccgg ggccatattt ctcggctcca tgtccactga
     1021 agcgttcggg gattatatcg ccgggccgag ccacgtcctc ccgacagggg gagccgcaag
     1081 gttctcgtcg cctctaagcg tctacgattt tctccgcatg ccgagcgtta tatccgtctc
     1141 cagaaagggg ttcgatacgc tttccgattc cgtcatgaat ctggcctaca gcgaagggct
     1201 cgaagcccac gccctctcgg tctccgtcag gacggacgtt gcggcgctca tccgtaagcc
     1261 ggagaattaa gcgaggaaaa aatcttcccg atactcaaca tgcccgataa atccaaggaa
     1321 aagggaaagt cgaaagccag aggtcttacg gaatcgaggg tcagggagga gataagagag
     1381 ctctcggcct actcggtccc tcacatcgag gccccggtca agctggacgg taacgagagt
     1441 cccttttccc ttccgccgga agtcggcgaa aaggtcatcg aaaagctgag gagcgtcgat
     1501 gtaaacaggt atcccgaccc cgaggcgctc gggctgaggg agcttatctc acgcataaat
     1561 gatttccccc tcggaggcat actactcggt aacggctcgg atgagctcat aggcatgctc
     1621 atcgccgcgt gctcgggcgg cactggcagg gttctctgcc ccgcgcccac gttctcgatg
     1681 taccgtataa caggggtagc actcggaagg gaggtgctcg aggccgggct cgaccgtaat
     1741 ttcgatctcg acatggaagc catgcctgct ctcatagacg agaaagaccc cgacctgatt
     1801 tttctcgcct ccccgaataa ccctacggga aacgcgtatt caagcgacag gattcgggag
     1861 ctgataaagc tctccaaagg agtggtcgta gtcgacgaag cctactccga tttcagcggt
     1921 cacactttcc tgcctctgat aaataaacac gagaacctcg tcatcctccg cactctctca
     1981 aaggtcggct tcgcggggtt aaggctcggc atactctacg ggagagagga gctcgttcgc
     2041 gagataaaca aggtcagata cccctacaac ataaactccc taacgcaggc tgcggccggg
     2101 gtcgtcctcg ggaaccacga attcgtcacc gaaaacatac agctcatcgt gagggagagg
     2161 gacagggtct ataaggccct ttccggaatg aaaggcgtcg aggcgttcag gagcgacgcc
     2221 aattttattc ttttcagggt cggcgacgcc gactccgtct tcaaagccct cgtagagagg
     2281 ggagtgctca ttaggaattt caacagaccc ggcaggctcg aaaattgtat gcgtgtgaca
     2341 atcggcacgc cttacgagaa cgactcattc ctcaacgcgc tcggaagcgc tctttcctct
     2401 taaccaaacg atcacggcat aacctgccca gaatacgaga cacgcgatag ggaacacgtc
     2461 ccccaatttt gtgtataagg taagccctcc gttcatgagc cccacttcac tctcgaggtt
     2521 tgtcctgacg aaaataggcg tcttcgcgac gaccctcccg accgggtcta taacggcgct
     2581 tatacctgta ttcgtcgagc gcagcaggta tctcctcgtc tcgacgctcc tggggatgga
     2641 tacgaacatg tgctgatacg gggcgacagt cctcccaaac cacgcgtcgt tcgtaatgtt
     2701 cacgatcagg ttggcgccct tctcgacgaa cctcctgctg aacgaaggga taatgtcctc
     2761 gtagcagatt atgggggcta tcctcgcccc cttgtcctct atctcgaaca ggttaagctc
     2821 cctgccgggg gtaaaatcgc ccgacgcggg gcttatgctt ttcagggccg gtatcaggtt
     2881 cgtgaacggc aggtactcgc cgaagaggag cagcttgatc ttatgatacc ttcccatgat
     2941 caccccgtcc gaattggcta ggaaagcggt gttgtatttg aggaggtcgt cgtccgactt
     3001 cacctcgccg ttcgggtttt tgggcgtgaa cgacataccg cccacgatga aatatttccc
     3061 gtccatcttc gggacgccca tctcctctct tacgagtaga taatcagatt cgatcggaaa
     3121 ccatgcctgt atggcggtct cgggccatat tatcaggtcg tccgacttga ggtcgatgga
     3181 catctccttg tgcctcttcg tcaccacctc ttcctggctc tccgactttt cgaggaaatc
     3241 gaaattggcc tgcaccatcc ctatcttgag cttgggcgct cgggctatct ccccgtcgac
     3301 ggtccctatc ctgatgtaac cgtagacgag ggtcgatata accagtgcga gagcgagcac
     3361 gatctccccc gcaggaaagc gggattcccc cgtcagattt ttcaaaaccc tcataagcgc
     3421 gaggttgacc acgactataa tgaagctcag agcatagacc ccgaacaggt cgaatatctg
     3481 aatgacgggt atgtaatcgg cctgcgaatt tgcgatgccg tacggaaaga ggaacgggaa
     3541 gaagtattca atagaagtcc atacggcggc tacgaggagc gctgacacga ctcccggacg
     3601 cctcaggaac ccgagcctcg tgacgatata cgagaatatg gcgtaggaga ggcccgagaa
     3661 cgcgctcagc accgcgataa agagcacgct cactaaaatc gggaatccgc cgaacctgct
     3721 gagagtgcct atgagccagt acgtgcccgc gaagttggag acgagccccg ccagcaggcc
     3781 gagcttgaat gcgtctttcg cggacttgtt atctatcgcc caaaataggg ggatgaggag
     3841 tacccaggcg aggatacccg tgtgcgggat catgaacgta agcgggtaaa gcacccctgt
     3901 gagcgccgca aggacgagat cgatcccgct tatccttttc atggtcgata aaagataccc
     3961 aacgcgcgtg ttatgggaag tcagccgcgt atcaccgaat gcgggcgggc ggggattgcc
     4021 tggttaagcc tttttcttct ttacgggttg tttagggctt gcgggcgcga gctcggtctc
     4081 tccagccgag tggagcctgt agagcttgac cgcgccgact atgataataa caactgccat
     4141 gacttgagcg accgagagtc ccggtatcgg gctcggagtc gtgctcctca ggaactctat
     4201 gaagaatctt tcgagtcccg ccagtatgag gtatatcgaa aacagccagc cggcgggcct
     4261 ttccagtttc cttatcttcc atatgaagag gaaaactatg gacataataa tgatctcgta
     4321 cacctgcgtc ggatgcacgg gaacggtcgt cggagggagc cccttaggaa acggtatggc
     4381 ccaggggaga ttggacggaa cgccgtaatc gtcccccacg aggaagcacc cgatgcgtcc
     4441 gatagcgtag gcgagcgcga gagccggggc ggcgaggtcg caaacaaccc acatgctcag
     4501 cttgttcctg agagcaatga accatactat tgctatggcc ccgaagaacc ccccgtaaaa
     4561 tgtgagtcca cccctcgata acaggttctc gagcggatgg ctcacgatgt ccgaaagcgg
     4621 gacgttctcg aacacgtaga gcagcttcgc tccaatgatc ccgcccgcca tggtcgcgag
     4681 gaacaggttc cccacgagct tttcgttgag tcccttcctc tttgcctcga gcgttat
//
LOCUS       DASSJH010000311        11562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1260183, whole genome shotgun sequence.
ACCESSION   DASSJH010000311 DASSJH010000000
VERSION     DASSJH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260183"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..690
                     /locus_tag="VFJ67_06670"
     CDS             193..690
                     /locus_tag="VFJ67_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HET7290108.1"
                     /translation="MSFGKMIAGLFKKDAAQTDTGNQGSSAGGESIGTSGKDLEAPEL
                     QTSETPANEAAGNSPSAGSPDGEITENKILEVLSNVYDPEIPIDIVNLGLIYGIDIDG
                     GNVRISMTMTAPGCPASTQIAGESKILVGEIPGVENVEIEIVWDPPWDPSKMSEEAQD
                     SMGIF"
     gene            739..1479
                     /gene="bioD"
                     /locus_tag="VFJ67_06675"
     CDS             739..1479
                     /gene="bioD"
                     /locus_tag="VFJ67_06675"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET7290109.1"
                     /translation="MPPLEDGVFVTGTDTGVGKTVIAAALAWSLSCAGRSVAVMKPVQ
                     TGMSLPGTADIGFVENVLGTKYDPSDVCIYRLSEPLSPLAASEISGIKIDTGKIIEAY
                     RRLRSAHDVVIVEGAGGLLVPITDNYLMSDLASDLGLPLLIVTRPGLGTLNHTSLTVE
                     SARKRGIDVLGVVINRFPASPGLAERTNPEQITRMTGAPIVGVFPDDPTISVEGGEAG
                     DIRELARAALAPALGGLFDLREFLGRLR"
     gene            1676..2251
                     /locus_tag="VFJ67_06680"
     CDS             1676..2251
                     /locus_tag="VFJ67_06680"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7290110.1"
                     /translation="MRKRITLLSAPVLTLVIFLGLYFFDNAESSIKGPAQPIRFSHKI
                     HAGADGDQLPCQYCHSFVDVSPIPGIPSVKKCMGCHTHIAGRDVEYDFDGTTINIQQE
                     IAKVREFWAKKEPIPWNKVNSMPGYVHFNHKRHIKRGFQCAACHGDVANMNQVYQAER
                     LNMGFCITCHTQNAKNHEELTHLKDCLTCHY"
     gene            2264..5200
                     /locus_tag="VFJ67_06685"
     CDS             2264..5200
                     /locus_tag="VFJ67_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7290111.1"
                     /translation="MSQDKKGGIKRRDFLKIIGVAGGTAAVSGACSNPAEQLIPYVIP
                     PEGIIPGVPNWYASTCRECPAGCGIVVKNREGRAIKVEGNPKNPINMGGSCAEGQAML
                     QGLYNPDRIRSPLLRNDMGRLEPLGWDQAQKTLAEKIQAVRSKGGSGKIVFLSDHIEG
                     TLGNLIGDWMSALGGQHLTYEVYSYEPLKEANRIVFGIDKIPTYNIDKTKYLLSFGAD
                     FLETWLSPTLYSRQFSEMHSYKDESMGKFVYVDPRAGLTGVNSDEWVSIKPGTEAVLA
                     LGIANVIVNGGMSPNGGGGFAGLLGGYTPEKVSSVTEVPVDTIKKIAKEFSSAQPSLA
                     IGGGIANTATNATETQVAINILNYVAGNVGQTIDFGNPLTLSTVSSFKEISSLVDSMK
                     SGQVELLFVYNVNPVFTLPKSLGFEDALGKVPNVVSFSSFMDETTEKAGLLLPDNTTL
                     ESWGDFVPDSRVHGLIQPVMTPVFNTKGTGDVILAVAAQLEGLKGRYTQTTYYDYLRD
                     SWKQAWTSVSGGGDFETFWKDALENGGFYRETTPAQVSVSGNVSGINFSEPEFKGDGD
                     FYFVAYPSYKYYDGRGANKPWLQELPDAITTGVWDSWVEMHPDTAGKLGVKLGDFVKI
                     ESPGGTIETQAYIYEGIRPDTLAVMIGQGHTSYGRYAKDRGVNPITILPVSTDRITGS
                     FAWLTTRVKASGTGRHAQFVQTQYTTTQHDRDVAQMVTLTELEGGLHRDEEKEPDFYP
                     PVEYARYQWGMSIDLQKCTGCGACVTACYAENNIPFVGKELVAKRRDMAWLRIERYFE
                     KSDDGGGFQTRFLPMLCQQCNNAPCEPVCPVFATYHNPEGLNGMVYNRCVGTRYCSNN
                     CTYSVRKFNWFSYKLPDPLNWQYNPDVTVRDKGVMEKCTFCIQRINYAKDQAKDKGRD
                     VLDGEFQVACQQSCASGAIVFGNLKDEASGVSKLSHDERGYQVLHQLNTQPNITYLKK
                     VKWDKA"
     gene            5200..6537
                     /gene="nrfD"
                     /locus_tag="VFJ67_06690"
     CDS             5200..6537
                     /gene="nrfD"
                     /locus_tag="VFJ67_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7290112.1"
                     /translation="MSDQTALTYTKVNQDVISMLDKPTIKWLALFLPVLGFVGFGLFC
                     FTYQVYTGLGVAGYRHPVFWGVYITDFVFWVGIAHSGTLISAILYLFRAKFRVSIYRI
                     AEAMTVFAVLTAGLFPIIHLGRPWFFYWLFPYPNQRELWVNFKSPLLWDVFAVSTYLT
                     VSSVFFFVGMIPDIAAIRDKVVEVPRKIAYSILSLGWKGSNWEWLHYSRAYLYFAAFA
                     TPLVLSVHSVVSWDFAMADLPGWHTTIFAPYFVAGAIFSGLGMVITLTIPIRKIFALE
                     DYITLDNYDGMAKMILLTSLIVGYAYGCEFFMAWYSGSPAEWGQFYYRATGQYALFYW
                     IMVVCNVVMPLPLWFKSVRRNIKALFIISIFINIGMWFERFNIIVISLSQGFEPGAWG
                     IYKVSWVEMGITIGSFAWFFMFFLIFIKTLPAISIAELKEILPVPKKEAKHHG"
     gene            6530..7039
                     /locus_tag="VFJ67_06695"
     CDS             6530..7039
                     /locus_tag="VFJ67_06695"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET7290113.1"
                     /translation="MDNQGVLGIFSYVDLTVKAVRKLKDEGFKNLRVFSPVPNHEIED
                     AVAAPESIVRFFTLFGAMLGTACGVGFTVLTSSSWPIPVSAKPIVSIPPYIVIMFELT
                     ILMGALSTLLGLLINSRLRRNAPRSMYDPRFTDDKFGVMVTCGKDKVKKAEEILNSEG
                     AEEIKFDGV"
     gene            7077..7634
                     /locus_tag="VFJ67_06700"
     CDS             7077..7634
                     /locus_tag="VFJ67_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7290114.1"
                     /translation="MKNKKYYAVLIIALSFMGATGAGAFPWNFDMWIQPSILPYEEPI
                     AYPDLSVSTTGLRIEPLPREHYEGMTVNPVPATAESLKNGEHLFNTYCFVCHGKEGKG
                     DGPVIKRGFYPMNLTNPAVIARTNGYIYAYIRYGGKVMMPSYRESITSDGAWDVVNYV
                     RKLQGNPNTEPAKPQNGNLPQETAQ"
     gene            7636..8880
                     /locus_tag="VFJ67_06705"
     CDS             7636..8880
                     /locus_tag="VFJ67_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290115.1"
                     /translation="MDQQIRDEILAKRSGIPTLGIYIFLIIAVIGFGTFVSQILGSDP
                     KSAWEIFLINFLFWTGIAQAGVVFSCILRITNARWARPFLRVSEGLGSFLPISLILLL
                     TVFAGRDYILPYATEHYHHPKDVWLNMTFVFGRNFIGFIVLLVLTLCYLYHSLRQDLG
                     GFGGKLDGLAGWVSSGWTGEEERKRIWVRIGKFAPAVIILYALVFSFFAWDFMMSLDP
                     HWFSTLFGPFYFMASFVASIGMTIILSIFLRRRLGLEEYLNDNQYHDIGKLLQGFSLF
                     WVYLFFSQLLPIWYANMPEETAFVIKRVQEEPFRSLAWAVLTCCFIFPFVSLIPRTNK
                     IVKPIVVFIASVSLTGFWLEKYVLVMPSLSNTINIGLMQILITLGFLGAFVMTFLIFM
                     RAFPSIPVGDPFFGGKSGSGGH"
     gene            8880..9023
                     /locus_tag="VFJ67_06710"
     CDS             8880..9023
                     /locus_tag="VFJ67_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290116.1"
                     /translation="MDQRHLDTITQVVVAALCLGVAGFLSLIATVALFGPHIAPLMNK
                     LPW"
     gene            9197..10021
                     /locus_tag="VFJ67_06715"
     CDS             9197..10021
                     /locus_tag="VFJ67_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7290117.1"
                     /translation="MAKKPSASAKKKNGLKFTYILYEKKDRIARVTINRPEVRNALNS
                     EVREELAIAIEDSWLDDDIGVIVLTGAGGKAFSAGGDLSWIVDPDKKVDAKYMLVHYR
                     LATAMRSCGKPIIARVDGYCVGGGNELNMLCDLTVASDTSVFAQAGPLVGSAPIWYGL
                     QQLQHSVGDKKAREIVYLCNRYTAQEAMDMGWVNKVVPKEKLDEEVDAWCQSLLEKSR
                     QSLRIAKLQINFASDMAHPQITHGLELARFFLKAPEMVEGATAFLEKRKPDFWGVD"
     gene            10045..10389
                     /gene="gldC"
                     /locus_tag="VFJ67_06720"
     CDS             10045..10389
                     /gene="gldC"
                     /locus_tag="VFJ67_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HET7290118.1"
                     /translation="MSKEAEIKFLIKLDDKGVPGDIYWSASESGIEGYVPCDSLMISV
                     WDRSEKNTMSVDLWTNRMQVGEMNAHFYFSLMKMADTYGRATGNQELSAMIRDFAGEF
                     AKKLEEFTVKDG"
     gene            complement(10406..11335)
                     /locus_tag="VFJ67_06725"
     CDS             complement(10406..11335)
                     /locus_tag="VFJ67_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET7290119.1"
                     /translation="MPRIDFGVMLRQQKIAFDDISKTAGLCDELGYHSVWFYDHILGM
                     GGIDLDIFESWTLMSALSTVTSRVRLGTMVLCNSFRPPPLLAKMGSSLDVISGGRLEF
                     AIGAGWFEPEYRAYGYDFPDSVTRIRQLSESVRVIKAMWTEEKPSFAGEYYCIEDAYC
                     NPKPVQKPHPPVIMGGAGEKYLLRAVAELADEWNCPANHSLSLERKLEALKGHCIAVG
                     RDPDEIKISQQTVCVLAKDEAELAEKLPKAQMRYGFFGDVGKLGIVGTPEECIRKIEE
                     NEKKGVTKYTIFFSDIMNHDTLRFFAREVMSAF"
BASE COUNT         2730 a         3015 c         3236 g         2581 t
ORIGIN      
        1 cacaggagcc cgattacgaa gcggcactca gggcggtaaa ggaatctctt tagttaacaa
       61 gctcatagca ggagcacgta tccgcaacat cagatgcgcg gccctcaccc cggtgcgctc
      121 tcgcctttaa aatccggttc ccggtatgta tccttatatt ggtaaggcaa tttatgctga
      181 ggaggctcgg tcatgagttt cggcaagatg atcgccggtc tttttaaaaa agatgccgcc
      241 caaacggata caggtaatca aggcagctct gcgggcgggg aaagtatagg aacgagcggc
      301 aaggacttgg aagctccgga attgcaaact tccgaaacac ccgccaatga agccgccggg
      361 aattctcctt cggcaggctc tcctgacggc gaaatcaccg agaataaaat cctcgaggtc
      421 ttaagcaacg tgtacgaccc ggagataccg atcgatatag tcaacctcgg gctgatatac
      481 ggcatagaca tagacggcgg aaacgtgcgc ataagcatga ccatgaccgc gcccgggtgt
      541 cccgcatcga cgcagattgc gggcgagtcg aagatactgg tcggggagat accgggagtt
      601 gaaaacgtgg aaatcgaaat tgtgtgggac ccgccctggg acccgagtaa aatgagcgaa
      661 gaggctcagg acagcatggg aatattttga tagccgtatc tttcgttaat atccttcacg
      721 caaggttgca ataagcgaat gccccccctc gaagacggag tttttgttac cggtacggat
      781 acgggtgtag gtaagaccgt tatagccgcg gccctggcct ggagcctgtc gtgtgcgggc
      841 aggagcgtgg cggtaatgaa acctgttcag accggcatgt cgcttccggg tacggcggac
      901 atcgggttcg tggagaacgt gctcgggacg aaatacgatc cctcggacgt atgtatatac
      961 agattgagcg agccgctgtc gccgctcgcc gcgtcggaaa tatcgggaat aaaaatagac
     1021 acgggaaaga ttatagaggc ctaccgcagg ctccgttccg cgcatgacgt ggtcatcgtg
     1081 gaaggcgcgg gaggtctgct cgtccctatt acggataact atctgatgtc tgacctggct
     1141 tccgacctcg gactccctct cctcatcgtc acccgccccg gtctcggcac actgaaccac
     1201 acgtccctca cggtagagtc cgcgagaaaa aggggcatag acgttctcgg tgtcgtcatc
     1261 aaccgcttcc ctgcctcccc gggactggcc gagcgcacga acccggagca aatcaccagg
     1321 atgacaggag cgcctatcgt gggtgtgttt ccggatgacc ctacgatctc cgtcgaggga
     1381 ggcgaagccg gcgatataag ggagcttgcg cgagcggctc ttgcccccgc cctgggcgga
     1441 ttattcgacc tccgggaatt tctcggccgg ctgagataga ccctgatttt aacccctgaa
     1501 aaatcgaaat atcgcgtcat cgcctgtgcg catatgtgtc cgcagggatt cgcaatttaa
     1561 ggtccggtgc gctaaacttg acagatttcc tcggtatgat atacatacta ggcgtttttt
     1621 gagtactttg atttaacttt ccaaatggac acactccaga gctaaggtaa ataggatgag
     1681 aaaacggatt acattgcttt cagcgcctgt tctgacactg gttatatttc tgggtcttta
     1741 ttttttcgat aacgctgaaa gcagcattaa agggcccgct cagccgatca ggttcagtca
     1801 taaaattcac gcaggcgccg atggcgatca gctcccctgt cagtactgcc acagcttcgt
     1861 ggacgtgtcc ccgattcccg gaataccgtc ggtcaagaaa tgcatgggct gtcacaccca
     1921 catagcaggc cgtgacgttg aatacgactt cgacggaacg actataaata ttcagcagga
     1981 gatagccaag gtcagggaat tttgggcgaa gaaagagcct attccctgga acaaggtcaa
     2041 ttctatgccc ggttacgtcc atttcaacca caagaggcac ataaagcgcg ggtttcagtg
     2101 cgcagcctgt cacggggacg ttgcgaacat gaaccaggtc tatcaggcag agaggctcaa
     2161 catggggttc tgtataactt gtcataccca gaacgccaaa aatcacgaag agctgactca
     2221 cctgaaagac tgtctcacgt gtcattatta attggaggag gaaatgtctc aagataaaaa
     2281 gggcggaata aagagaaggg atttcctgaa gatcatagga gtggccggag gtacggctgc
     2341 tgtatcgggc gcgtgctcca atcctgccga gcaattgatc ccttacgtca tcccgcccga
     2401 agggattatc cccggtgtgc ccaactggta cgcgagcacc tgccgggagt gtcctgccgg
     2461 ctgcggtata gtggtgaaga accgcgaagg cagggctatc aaggtcgaag ggaatcccaa
     2521 gaatcccatt aacatgggag ggagctgcgc cgaagggcag gcgatgctcc agggactgta
     2581 taacccggac cgtataaggt ccccgctcct ccggaacgac atgggaaggc tcgagccgct
     2641 agggtgggac caggcgcaga aaacgctcgc cgaaaaaata caggcggtca ggagtaaagg
     2701 cggcagcggg aagattgtct ttctctccga ccacatcgaa ggaaccctgg ggaacctcat
     2761 cggtgactgg atgtccgcgc tcgggggaca gcacctgaca tacgaagtct actcgtacga
     2821 acctctcaag gaagctaacc gcatagtatt cggcatagat aagataccta catataatat
     2881 agataagacg aagtaccttc tctcgttcgg cgcggatttc ctcgaaacgt ggctgtcgcc
     2941 gaccctctac tcaaggcagt tcagcgagat gcactcatat aaagacgaat ccatgggtaa
     3001 gttcgtctac gtggatccga gagccgggct tacgggcgta aactcggacg aatgggtctc
     3061 cataaaaccc ggcactgaag ccgtcctcgc gctcggtatc gccaacgtga tcgtgaatgg
     3121 cgggatgtcg cctaacggcg gaggagggtt cgcggggctc ctcggaggat acacgcccga
     3181 gaaggtttct tcagtcacgg aagtgcccgt cgatacgata aagaagatcg cgaaggagtt
     3241 ctcctccgcg cagccgagcc tggctatagg cgggggcata gcgaataccg cgaccaacgc
     3301 cacggaaacg caagtcgcaa taaatatact taactacgtc gccggcaatg tcggtcagac
     3361 gatcgatttc gggaacccgc tcacgctctc gactgtaagc tcgttcaagg agatttcatc
     3421 gctcgtggat tcgatgaaga gcgggcaggt cgagctctta ttcgtttaca acgtaaatcc
     3481 ggtgttcact cttccgaagt cgctcgggtt cgaagacgcg ctcggcaagg tcccgaacgt
     3541 ggtcagcttc tcgagcttca tggacgagac gactgagaag gccggtctct tactcccgga
     3601 caacacgacc ctcgaatcgt ggggggattt cgtccccgac agcagagtcc acgggctcat
     3661 tcagcccgtt atgacccctg tattcaacac taagggcacg ggggacgtaa tactcgccgt
     3721 ggccgcacag ctcgaagggc ttaagggcag atacacacag accacctact acgattatct
     3781 cagggattca tggaaacagg catggacgag cgtttcggga ggaggcgatt tcgagacctt
     3841 ctggaaagac gctctcgaga acggcggatt ttacagagaa acgacacccg cgcaggtatc
     3901 tgtgtcgggt aatgtttcgg gaattaattt cagcgagccc gagtttaaag gagacggcga
     3961 tttctacttc gtcgcatacc cgtcatacaa atactacgac ggaagagggg cgaacaagcc
     4021 ctggctccag gagctccccg acgcgatcac tacgggggtc tgggactcgt gggtagagat
     4081 gcaccccgat acggccggga agctcggtgt caagctcggg gatttcgtga agatagaatc
     4141 gcccggcgga accatagaaa cgcaggcata tatatacgaa ggaataaggc ccgatacgct
     4201 agccgttatg ataggacagg gccatacgag ttacggcaga tacgcgaagg acaggggcgt
     4261 aaaccccatc actatcctcc ctgtctcaac ggacaggatc acggggagct tcgcatggct
     4321 tacgaccagg gttaaagctt ccggcacggg caggcacgca caattcgtac agacccaata
     4381 cactaccact cagcacgaca gggacgttgc gcagatggtc acgctcacgg agctcgaggg
     4441 cggactgcat cgggatgagg aaaaggagcc ggacttctat ccgcctgtcg aatacgcgag
     4501 gtaccagtgg ggtatgtcca ttgacctcca gaaatgcacg ggatgcggag cgtgcgttac
     4561 ggcgtgttac gccgagaaca atatcccctt tgtcggcaag gagctcgtcg ccaagagacg
     4621 cgacatggcg tggctaagaa tagagaggta tttcgagaaa tcggacgacg gcggcggttt
     4681 ccagacgagg ttcctcccca tgctctgcca gcagtgcaac aacgcgccct gcgagccggt
     4741 gtgccccgtg tttgctacat atcacaaccc cgaagggctt aacgggatgg tctataacag
     4801 gtgcgtcggt acgaggtact gctccaacaa ctgcacatac agcgtaagga aattcaactg
     4861 gttctcgtac aagctccccg atcccctcaa ctggcagtat aaccccgacg tcacggtcag
     4921 ggacaaggga gtcatggaga agtgcacgtt ctgtatccag aggatcaact acgcgaagga
     4981 ccaggcgaag gacaagggcc gggacgttct cgacggagag ttccaggtag cgtgccagca
     5041 gtcatgcgcc tcgggcgcga ttgtcttcgg gaacctcaag gacgaggcgt ccggagtgtc
     5101 gaagctttcg cacgatgaaa ggggctatca agtgctccac cagctcaaca cgcagccgaa
     5161 cattacgtat ctaaaaaaag tcaaatggga taaggcataa tgagtgacca gacagcatta
     5221 acctatacga aagttaacca agatgtaata agtatgctcg ataagcccac tatcaagtgg
     5281 cttgcgcttt tcctgccggt cctcgggttt gtcggcttcg gtttgttctg cttcacttat
     5341 caggtatata caggcctggg ggtcgcggga tacagacacc cggtattctg gggcgtttac
     5401 ataacggact tcgtattctg ggtcggtatc gcccactcgg gaaccctgat ctcggcaatc
     5461 ctctacctgt tcagggcgaa attcagggta agcatataca ggatcgccga ggctatgacg
     5521 gtgtttgcgg ttctgaccgc gggccttttc cctataatcc acttaggccg cccgtggttt
     5581 ttctactggc tcttcccata cccaaaccag cgcgagctgt gggtgaactt caagtcgccg
     5641 ctcctctggg acgtgttcgc tgtcagcaca taccttaccg tgagctctgt gttcttcttc
     5701 gtgggaatga tccccgacat agcggcgata agggacaagg tggtcgaagt accgaggaag
     5761 atcgcctact cgatcctatc gctggggtgg aagggctcca actgggagtg gctccattat
     5821 tctagggcct atctttattt cgccgccttc gccacaccgc tcgttctctc ggtgcacagc
     5881 gtcgtatcat gggacttcgc aatggctgac ctgccagggt ggcacaccac gatcttcgcg
     5941 ccatatttcg tcgccggggc catattctcg gggctcggaa tggttataac gctgacgatt
     6001 ccgataagaa agatcttcgc tctcgaggat tacattacgc tcgataatta cgacggaatg
     6061 gcgaagatga tattgctgac gtccctgatc gtgggatacg cgtacggctg cgaattcttc
     6121 atggcttggt acagcgggag tcctgccgaa tggggtcagt tctattacag ggccaccggt
     6181 caatacgcgc tcttttactg gataatggtc gtttgcaacg ttgtaatgcc gctcccgctc
     6241 tggttcaaga gcgtaaggcg caatataaag gcgcttttca taatctcgat cttcataaat
     6301 atcggtatgt ggtttgaaag gttcaatatc atcgtaatat ccctctcaca gggattcgag
     6361 cccggagcat ggggtatata taaggtgtcg tgggtcgaaa tgggaataac cataggcagt
     6421 tttgcctggt tctttatgtt cttccttatt ttcatcaaga ccctgcccgc gatctcgatc
     6481 gcggagctga aggaaatttt gcctgtgccg aagaaggagg caaaacacca tggataatca
     6541 gggagttctc gggatattct catacgtcga tctaaccgtt aaagctgtca ggaagctgaa
     6601 ggacgagggg ttcaagaacc ttcgtgtctt ttcccccgtg cctaaccatg agatcgaaga
     6661 tgcggtcgcg gccccggaaa gcatagtacg gttctttaca ctgttcggcg ccatgctcgg
     6721 taccgcgtgc ggcgtgggat ttacggtact tacgtcgtcc tcctggccga taccggtaag
     6781 cgcgaagccg atagtatcga taccgcctta tatagtaata atgtttgagc tcacgattct
     6841 tatgggtgcg ctttctacgc ttctcgggct tctcattaat tcacgtctca ggcggaacgc
     6901 tcccaggagc atgtacgatc cgaggtttac cgacgacaag ttcggcgtaa tggttacatg
     6961 cggcaaggat aaggttaaga aggccgaaga gatattaaat tccgaggggg ccgaagaaat
     7021 aaagttcgac ggtgtataga ccgccgcgca cctcaggagg tactacggga ttgtcagtga
     7081 agaataagaa atattatgcc gtgttgataa tcgcattgag cttcatgggc gcaaccggtg
     7141 cgggcgcatt cccctggaat ttcgatatgt ggattcagcc ttcgatactg ccttatgaag
     7201 agccgatcgc ttacccggac ctgtcggttt cgaccaccgg cctcaggatc gagcctctcc
     7261 ccagggagca ctacgagggc atgaccgtga atcccgtgcc cgctacggcc gagtcgctca
     7321 agaacggcga gcatctcttt aatacatatt gtttcgtctg tcacggcaaa gagggcaaag
     7381 gggacgggcc cgtaatcaag agggggttct atccgatgaa cctcaccaac cccgccgtca
     7441 tagcccggac caacggctac atatacgcct acatcagata cggcggcaag gtgatgatgc
     7501 cgagctacag ggaaagcatt acttccgacg gcgcgtggga cgtagtgaat tacgtaagga
     7561 agcttcaggg taacccgaat accgaaccgg ccaagcccca gaacgggaat ttaccacagg
     7621 agaccgctca gtaatatgga tcagcagata agagacgaaa tactggcaaa aagatcgggc
     7681 atcccgactt taggcatata catattcctc atcatagccg tgatcggttt cggcacgttc
     7741 gtgtctcaga tcctggggtc tgatccgaag agcgcatggg agatattcct gataaatttc
     7801 cttttctgga ccggcatagc gcaggcgggc gtcgtgttct catgcatact ccgcataacc
     7861 aacgcgaggt gggcaaggcc gtttctgcgc gtgagcgagg gactaggctc gttcctgccg
     7921 atatccctga tactacttct gacggttttc gccggcaggg actacatact cccttacgcg
     7981 accgagcatt atcaccaccc gaaggacgta tggcttaata tgacttttgt attcgggcgc
     8041 aattttatcg ggttcatagt tcttcttgtg ctgaccttgt gctacctcta ccattcgctc
     8101 aggcaggacc tcggcgggtt cggcggcaag ctcgacgggc ttgcgggatg ggtctcctcc
     8161 ggctggacgg gagaggaaga gagaaagagg atatgggtca ggatagggaa attcgccccg
     8221 gcagtcatta ttttatacgc tctcgttttc agcttcttcg cgtgggactt catgatgtcg
     8281 ctcgaccccc actggttcag cacgttgttc ggcccgtttt atttcatggc gagcttcgtt
     8341 gcgtccatcg ggatgactat catcctttcg atattcctca ggaggaggct cggcctcgag
     8401 gagtatctga acgataacca gtatcacgat atcggtaagc tgctccaggg tttctcactc
     8461 ttctgggttt acctgttctt ctcgcagcta ctcccgatat ggtacgcgaa tatgccggaa
     8521 gagactgctt tcgttataaa gagggttcag gaggagccgt ttagatccct ggcgtgggcc
     8581 gtgctgacct gctgcttcat tttcccgttc gtatctctca tcccgagaac gaacaagatc
     8641 gtgaagccca tagtggtttt catcgcatcc gtatccctga ccgggttctg gctcgaaaag
     8701 tatgtgctcg tgatgccgtc cctatcaaat actattaaca taggattaat gcagatactg
     8761 ataaccctcg ggttcctcgg ggcgttcgtc atgacgttcc ttattttcat gagggcgttt
     8821 ccgtccatac cggtcggcga cccgttcttc ggcggtaaat caggctctgg agggcattaa
     8881 tggaccaaag acatttggat actatcactc aggttgtcgt agcggcactc tgtttgggcg
     8941 ttgcgggttt tttatcgctc atagcgaccg tcgccctttt cggaccgcat atcgctcccc
     9001 tgatgaacaa gctgccctgg taaccggggc aatacgtgga ttgtgcgagt ggctcgtgct
     9061 gcgaccttgg gcctcgtcgc gttttttgta ataaatgctg tcccgcccac cccaaccgat
     9121 aaaatcattt gacagaccgt attatccggt gcgaaaatca taattacaaa aatccgaatt
     9181 atgtcaggag gtcattatgg ctaaaaaacc gtcggcttcg gcaaagaaga aaaacggcct
     9241 taaattcact tacattctat atgaaaagaa ggacaggata gcgcgcgtga cgataaaccg
     9301 tcccgaggtg aggaacgcgc ttaactcgga ggtgcgggag gagctcgcta tagctataga
     9361 ggactcatgg ctcgacgacg atataggcgt tatcgtcctc acgggcgcgg gcggaaaggc
     9421 attctcggca ggaggggacc tgtcctggat agtcgatccc gataaaaagg tggatgccaa
     9481 gtacatgctc gtccactaca ggctggccac ggccatgcgc tcttgcggta agccgattat
     9541 cgctagggtc gacggttact gtgtcggggg cgggaacgag ctgaatatgc tctgcgacct
     9601 gaccgtagct tccgacacgt ccgttttcgc gcaggcaggc cctctagtgg ggagcgcccc
     9661 tatctggtac ggcctccagc agctccagca ttcggtcggg gacaagaagg ccagggaaat
     9721 agtctatctc tgcaacagat acactgccca ggaagcgatg gacatgggct gggtcaataa
     9781 agttgtcccg aaggagaagc tcgacgaaga ggtggacgcc tggtgtcaga gccttctcga
     9841 gaagagcaga cagtcgctca ggatcgcgaa gctgcagata aacttcgcgt ccgacatggc
     9901 ccacccgcag ataacccacg ggctcgagct ggcgaggttc ttcctcaagg ctcccgagat
     9961 ggtcgaaggc gcgaccgcgt tcttggagaa gagaaagccc gatttttggg gggtcgattg
    10021 attatgcgca gtccgggggc atagatgtct aaagaggcgg aaataaagtt ccttataaag
    10081 ctcgacgaca agggcgtgcc gggggatata tactggagcg cgagcgagtc cggtatcgag
    10141 ggctacgtgc cctgcgattc gctgatgata tccgtgtggg acaggtccga gaagaacacc
    10201 atgagcgtcg atctctggac gaaccggatg caggtgggtg agatgaacgc tcacttctat
    10261 ttctcgctaa tgaagatggc cgatacgtac ggcagagcga ccggcaacca ggagctctct
    10321 gcaatgataa gggatttcgc gggggagttc gcgaagaaac ttgaggaatt cacggtcaag
    10381 gacggctgat tttcagtccc gcctctcaga atgcggacat tacctcgcga gcgaagaacc
    10441 tgagagtatc gtggttcatg atgtccgaga agaagatggt atatttcgtt acgcctttct
    10501 tctcgttctc ttcgatcttc cttatgcact cctcaggcgt tccgactatg cccagcttcc
    10561 ccacgtcacc gaagaacccg tacctcatct gagccttggg gagcttctct gcgagctccg
    10621 cctcgtcttt cgcgaggacg catacagtct gctgggatat cttgatctcg tccgggtccc
    10681 ttccgaccgc gatgcagtgg cctttcagcg cttcgagctt tctttctaaa gagagcgaat
    10741 ggttcgccgg gcagttccac tcgtcggcta actcggccac ggctctgagc agatactttt
    10801 cccccgctcc gcccatgatg acgggagggt ggggcttctg gacgggctta ggattacaat
    10861 aagcgtcctc aatgcagtaa tactcccccg cgaagctcgg cttttcctct gtccacatag
    10921 ccttgattac acgcactgac tcggacagct gccttattct tgtcacggag tcggggaaat
    10981 cgtatccgta agccctgtat tcgggctcga accagccagc tcctatggcg aactcgagcc
    11041 tccccccgct gatcacgtcg agcgaggacc ccatcttggc gagtagcggc gggggcctga
    11101 acgaattaca cagcaccatt gtgccgagtc ttaccctcga cgttaccgtc gagagggccg
    11161 acatcagggt ccatgactcg aagatgtcga ggtcgatacc ccccatgccg agtatatggt
    11221 cgtagaacca gaccgagtga tatccgagct cgtcgcagag cccggctgtt ttacttatat
    11281 cgtcgaatgc gatcttctgc tggcgcagca ttactccgaa atctattctg ggcatgtcga
    11341 ttccccccga tcccgtttac aggaagaatc aatttaacag agcatgaggg gggattcaaa
    11401 tatttcttcc ggagagtcgt tccggtttga cacccggcgc taaatactat aattttagtg
    11461 cccgataagg caggaggacc aaacaatatg gaaaaaggaa ttttaaatat gccagctgcg
    11521 gcgaaatcgg tttggttacg cacggcggca atcgttttgt ta
//
LOCUS       DASSJH010000312         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1152818, whole genome shotgun sequence.
ACCESSION   DASSJH010000312 DASSJH010000000
VERSION     DASSJH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152818"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1368
                     /locus_tag="VFJ67_06730"
     CDS             52..1368
                     /locus_tag="VFJ67_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7290120.1"
                     /translation="MKNSGNFILGGGITGLAAGLVSGLPVYESESVPGGICSSYYLTP
                     GSGDRLFKTPEDGEAYRFEIGGGHWIFGGDTAVLHFIRSLVPVKSYGRKSSVFFSKQG
                     LYVPYPIQNHLSFLGKDLATRAINEIINAPEGTPKTMADWLEMSFGKTLLELFFAPFH
                     ELYTAGLWTRIAPQDSYKSPVNLSVAVQGAIDKAPPPVGYNVTYVYPKEGLNTLAQRM
                     ARKSDVRYEKRVAKINVRKKEVQFSDGSGANYENLISTLPLNKALLMSGVKVEEKPAS
                     YTSVLVLNIGATRGKKCPDDHWLYIPDSDSRFHRVGFYSNVDVSFMPKSSRKENERVS
                     IYVEKAYQNGKKPSDKEIKQYSDSVVKELRSWGFISKAETVDPTWIDVAYTWSMPGSK
                     WKERALVELEKHGIYQIGRYGRWVFQGIAESIKDGFFIGGALGNKR"
     gene            1413..2354
                     /locus_tag="VFJ67_06735"
     CDS             1413..2354
                     /locus_tag="VFJ67_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET7290121.1"
                     /translation="MLERLSTRLKSTISLIFGASGVYIASDSAPPALLIAGRNLPVYI
                     DKNLHPCFKQNRAPIPHRVFILTVPKSGTYLIAKILENLGIVDCGVHIAIDHIQDNRF
                     ADKKVLKREAWKYYVPIPFKISTKLIKNGQFSFGHIPYYLEGEQMLHGFKKIFTFREL
                     RDIIISLVRYYDSREQNYAKPERRRLYDAFKAAPIGNEKFQAWYAMWGKEFADLIRNM
                     FPWKDRGDVFRVKFETLMGDEGREAQFTLLRGLGGFLELNFTDDMIDKALSDSIGAET
                     LTYSGKRSSYEDWWNDELEELFVRYGFKDLNGIYGYK"
     gene            complement(2446..3306)
                     /locus_tag="VFJ67_06740"
     CDS             complement(2446..3306)
                     /locus_tag="VFJ67_06740"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7290122.1"
                     /translation="MKIPNFFLVGAAKAGTTAVHVYLYQHPEVYMSFLKEPKYLSLPA
                     NKFPHMGPGDKKPDDETVKSMEEYMNLFKDAGNEKVIGESSADYLYFHKIVIPQIKTL
                     NPDAKILIMLRNLVERAYSSYRHMIMDRREKLSFENALRREEERMKENYEFIWFYKDV
                     GFYYEQVRHYIDSFGRKNVKICLYDDFAADLMAVMKKIFRFLDVDKGFDPDTAVKYNA
                     GPGVRSESLEKFLVEYDHSVKKVLRPLLLNTIGRKYTEAIVNYFINRNTLHIKPQTRK
                     RLTGTYESDI"
     gene            complement(3359..>3782)
                     /locus_tag="VFJ67_06745"
     CDS             complement(3359..>3782)
                     /locus_tag="VFJ67_06745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7290123.1"
                     /translation="ENTDLENLITWIPRMSQESLDVLKDKKFDLLVLDSDHRYDTAMW
                     ELINFEPMLNTGGHILMHDSIYFDGVGAAVKQLYNNPRFEVITLDTPRKSHLAGSRCP
                     GITIVRKLSDGEPALKFEEDFKGWFVGDRTAVPYLRRV"
BASE COUNT          943 a          955 c          948 g          936 t
ORIGIN      
        1 aatgtgtaag tatagagact tcattcgatg aaaaggagcg gcgctcagaa agtgaaaaat
       61 tccggaaatt ttatactcgg gggaggcatt acgggattgg cggccggtct cgtatcgggt
      121 cttcctgtat atgagtccga atcggtaccg ggcggcatat gctcgtccta ttatctgacc
      181 ccggggagcg gcgatcgctt gttcaagact ccagaagacg gagaagcata caggttcgag
      241 atagggggcg gacattggat attcggaggg gacaccgctg tgctccattt tataaggtca
      301 ctcgtaccgg tgaaatctta cggcagaaag tcttccgtct tcttcagcaa gcagggcctc
      361 tatgtccctt atcctataca gaaccacctg agcttcctcg gcaaggacct cgcaaccagg
      421 gcgataaacg agattataaa cgcccccgag ggtactccta agactatggc ggactggctc
      481 gagatgagct tcgggaagac gctgctggag ctgtttttcg ccccgtttca cgagctctac
      541 acggccggat tgtggaccag aatagctccc caggactcct acaaatcccc cgtgaacctc
      601 tcggtcgccg ttcagggcgc gatcgacaag gcgcctccgc cggtcgggta taacgtcacg
      661 tacgtatatc ccaaggaagg cctgaacact ctcgcgcaga ggatggcccg aaaaagcgac
      721 gtccggtatg aaaaacgcgt agcgaaaata aacgtcagaa aaaaagaggt ccagttctcg
      781 gacgggtcag gcgcgaatta tgaaaatctg atctccacgc tcccgctcaa taaggcactg
      841 ctcatgtcgg gtgtaaaggt cgaagaaaaa ccggcgagct acacttccgt gctcgtgctc
      901 aatataggcg cgacgcgcgg aaagaagtgc cctgatgacc attggctcta catccccgac
      961 agcgattccc gcttccacag agtagggttt tacagcaacg tcgacgtctc gttcatgccc
     1021 aaatcctccc ggaaggagaa cgagcgggta agcatctacg tggaaaaggc gtatcaaaac
     1081 ggaaagaagc cctccgataa agaaataaag cagtattcgg actccgtcgt aaaagagctc
     1141 aggtcgtggg gattcatatc taaagccgag actgtggacc ccacgtggat cgacgtcgcc
     1201 tacacgtggt cgatgcccgg ctcgaaatgg aaggagcgtg cgctcgtcga gctcgagaag
     1261 cacggtatat atcagatagg ccgctacggc cgctgggtct tccagggcat agcggaatcc
     1321 ataaaggacg gcttcttcat cggcggcgcg cttggaaata agcgttgaat ccaggctgcc
     1381 ggtcatgaaa tagacagtgc tctcgacaga aaatgcttga aagattaagc accaggctca
     1441 aatcaaccat aagtctgata ttcggcgcat cgggagtgta tatcgcgtcc gattctgcgc
     1501 ctcctgcttt gttgatagcc ggcaggaacc tgcctgtcta tatagataaa aacctgcatc
     1561 cgtgttttaa acaaaatcgg gcgcctatcc ctcatcgtgt attcattcta acggttccca
     1621 aatccggcac ttatcttatt gcgaagattc tcgagaatct ggggatcgtc gattgcggcg
     1681 tccacattgc gattgaccac atccaggaca accggttcgc ggacaagaaa gtgctcaaac
     1741 gcgaagcctg gaaatattac gtgcctatac cgtttaaaat atccacgaag cttataaaga
     1801 acgggcaatt ctcattcggc cacatccctt actatctgga aggcgagcag atgcttcacg
     1861 gattcaagaa gatatttacg ttcagggagc tccgcgacat aatcatctct ttggtcaggt
     1921 actacgattc gcgtgagcag aattacgcca agcccgagcg acggaggctt tacgacgcgt
     1981 tcaaagccgc gccgattgga aatgaaaaat ttcaggcatg gtacgcgatg tggggaaaag
     2041 aattcgctga cctcataagg aacatgttcc cgtggaaaga ccgcggcgac gtattccgtg
     2101 tcaagttcga gacactcatg ggcgacgaag gcagggaagc ccagttcact ctgttgaggg
     2161 ggttaggcgg tttcctagag cttaatttta cggacgacat gattgataaa gccctgagcg
     2221 actccatagg ggccgagaca ctcacctatt ccggaaaacg cagctcctac gaggattggt
     2281 ggaacgacga gctagaggag ttattcgtcc gctatggatt caaggatctc aacgggattt
     2341 acggctacaa gtagcgggcc gcgtagtcgg catttcatgt aaattgccga cgtaaagtta
     2401 ttcaagccat cccgagagat cacgctcaat cagaccttcc agttttcaaa tgtcgctctc
     2461 gtatgtgccg gtcaagcgct ttctcgtttg cggtttaatg tggagcgtat tcctgtttat
     2521 gaaataattg acgatggctt ctgtatattt tcttccgatc gtatttaata acagggggcg
     2581 gagcactttc ttgacagaat ggtcgtattc gaccaggaat ttttccaatg attcgcttcg
     2641 cacgccgggt cctgcgttat acttgacagc ggtgtctgga tcgaaaccct tgtccacgtc
     2701 gaggaacctg aatatctttt tcatgacagc cattaagtcc gcggcgaaat cgtcgtagag
     2761 gcatattttt acattcttcc tcccgaacga atcgatatag tgcctgacct gctcgtaata
     2821 aaaacccacg tctttgtaga accaaatgaa ttcgtagttc tctttcattc tttcctcttc
     2881 gcgccggagc gcattctcga acgagagttt ctctcttctg tccattatca tgtgacggta
     2941 cgatgaataa gccctctcaa caaggtttct gagcattatc aatatctttg catcggggtt
     3001 tagcgttttg atctgcggga taacgatttt gtggaaatag agatagtccg ctgaagattc
     3061 tcctatgacc ttctcgttcc ccgcgtcctt gaacaggttc atgtactctt ccatcgactt
     3121 tactgtctcg tcatcgggtt ttttgtcccc cggacccatg tgcggaaatt tgttcgcggg
     3181 taaggaaaga tatttgggtt ctttcagaaa gctcatatag acttcagggt gttggtaaag
     3241 atatacatgc acggctgtag tccctgcttt cgctgctcca acaagaaaaa aattaggaat
     3301 tttcaacttg gcaacccgga tggctcctat ctcttaactc tgtccgacca aacataacct
     3361 agacccgacg cagatacggg accgcggttc tatcgcccac gaaccatccc ttgaaatcct
     3421 cctcgaactt caacgccggc tctccgtcgg ataattttct cactatagtt atgcccggac
     3481 atctcgaacc ggccaggtgg ctctttctcg gcgtgtctag agtaatgacc tcgaagcgcg
     3541 gattattata cagctgcttt actgcggccc ctactccatc gaaatatatg ctgtcatgca
     3601 tcagtatatg tcctcccgta ttcagcatcg gctcgaaatt gatcagctcc cacatggctg
     3661 tatcgtatct gtggtcggag tctagcacca gtaggtcgaa tttcttatcc ttgaggacgt
     3721 caagggattc ctgagacatc ctggggatcc acgtgatgag gttctctaag tctgtgttct
     3781 cc
//
LOCUS       DASSJH010000313         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1587473, whole genome shotgun sequence.
ACCESSION   DASSJH010000313 DASSJH010000000
VERSION     DASSJH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587473"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VFJ67_06750"
     CDS             <1..1144
                     /locus_tag="VFJ67_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7290124.1"
                     /translation="ELVLEDFKNHHEIDLREDKMALQRIRETCEKAKQELSSIHETAI
                     NLPFIAVDQTGPKHLNYKINREEFERLVGELVDRLEIPCKQALEDARLDPSGIDEVVL
                     VGGMTRMPRIQEKVREIFKKEPQTRINPEEVVAIGASIQGGVLEGKVEEVLLLDVVPL
                     SLGVETRGGLFTKIIDRNTTIPTKRSRIFTTAFDNQEFVGIHVLQGERELVDGNISLT
                     NFNLIGIPPAPRGIPQIEVSFEVDADGILHVSAKDLGTGKKQAIQVVTSGGLSDDDIQ
                     SIISDSKQYSDTDKKRKDVALLKNEAEGLLYSVRRTLDSYGDKVDADLRDRLEQSIEK
                     MKDALTGSDYVYIKDVFDELKESSYKFAEVIYSRQEKTTEAHGGNE"
     gene            1169..2263
                     /gene="dnaJ"
                     /locus_tag="VFJ67_06755"
     CDS             1169..2263
                     /gene="dnaJ"
                     /locus_tag="VFJ67_06755"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7290125.1"
                     /translation="MESIDYYEILQVERSSTGEEIKRSYKRLAFEYHPDRNPGDREAE
                     EKFKKINEAYQILSDPNKRARYDSFGHISAEGIFSDIDFESGFGDIFGNLFEEVFNAG
                     GAEKARRGRDLKYSLDLTFEESIDGCEKELRIPRREPCRECGGSGAARGGEVVCTSCG
                     GRGELRYSQGLFAIKRACPTCGGTGKRITKPCPRCGGERFVLSEHQVKVKVPAGISDG
                     VRLRMRGEGDSGYLNGPSGDLYIEIHVEEHPLFRREEQDLYCTVPIEFVQAALGAEID
                     IPTPKGKSTIKIPPGTQSGQTFRLKGKGVPKLEGRGQGDLYIQVQVEVPVKLNKKQQL
                     LLEEFARASEEGNVPTVKKFVSRLQEILNK"
     gene            complement(2306..2581)
                     /locus_tag="VFJ67_06760"
     CDS             complement(2306..2581)
                     /locus_tag="VFJ67_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET7290126.1"
                     /translation="MPSVRIPTPLRKLTSDKDEVTINAANIAELIDQMEAQYPGIKSR
                     LCDETGNVRRFINLYVNNEDIRFLSGKETELKDGDIVSIIPAIAGGF"
     gene            complement(2550..>2837)
                     /locus_tag="VFJ67_06765"
     CDS             complement(2550..>2837)
                     /locus_tag="VFJ67_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290127.1"
                     /translation="LLAETEGIFTETAGGVTMAVTQKLISQGVIPKNESIVVSITGNG
                     LKTQEAIADSVSQPQIIEARLEEFEDLYQKIKEHEDKGGTKDALCSHSHTA"
BASE COUNT          722 a          762 c          831 g          522 t
ORIGIN      
        1 agagctcgtg ctcgaggact ttaagaacca tcacgagata gacctcaggg aagacaagat
       61 ggcgctccag aggataaggg agacgtgcga gaaggcgaaa caggaactct cttcgatcca
      121 cgagactgct ataaacctcc cgttcatcgc ggtcgaccag acgggcccca aacacctcaa
      181 ctacaagatc aacagggaag agttcgaacg cctggtcggg gagctcgtgg acaggcttga
      241 gataccctgc aaacaggcgc tcgaggacgc gaggctcgat ccttccggaa tagacgaagt
      301 ggtgctcgtc ggcggaatga cgcggatgcc gcggatacag gaaaaagtga gggagatttt
      361 caagaaggag ccccagactc gtatcaaccc tgaagaagtc gtcgccatag gcgcctccat
      421 acagggaggg gttctcgaag gcaaggtcga ggaggtgctc ctgctcgacg tcgttcccct
      481 ctcgctcgga gtcgagacga ggggcggcct cttcacgaag atcatagaca ggaacacgac
      541 cataccgacc aagaggagca ggatattcac gacggccttc gacaaccagg agtttgtcgg
      601 catccacgtg cttcagggcg aaagggagct cgtggacggc aacatatccc tcaccaactt
      661 caacctcatc ggcataccgc ctgcgccgag gggcatacct cagatcgagg tctccttcga
      721 agtggacgcg gacggcatac tgcacgtctc cgccaaagac ctcgggacgg gaaagaagca
      781 ggccatacag gtagtgacgt cgggcggcct gtcggacgac gatatacagt caataatatc
      841 cgacagcaaa cagtactcgg acaccgacaa gaaacgcaaa gacgtggcgc tcctcaagaa
      901 cgaagccgaa ggactactct attccgtcag gaggacgctc gactcttacg gcgacaaggt
      961 ggacgccgac ctcagggaca ggctcgagca atcgatcgaa aaaatgaagg atgctctcac
     1021 gggctcagat tacgtgtata ttaaagacgt attcgatgag ctgaaggagt cgtcatataa
     1081 attcgccgag gtcatatact ccaggcagga aaagaccacc gaagcgcacg gcggaaacga
     1141 ataacaatcc cgtacggatt catcattaat ggaaagtatc gactattatg aaattctcca
     1201 ggtcgaacgc agctccacag gcgaggaaat aaaaagatcg tacaagagac tcgcgttcga
     1261 ataccatccc gacagaaacc ccggcgacag ggaagccgaa gaaaaattca agaagataaa
     1321 cgaagcctac cagatcctga gcgacccgaa taaaagggcg cgctacgaca gcttcggcca
     1381 catctcggcc gaggggattt tctccgacat cgacttcgag tccggtttcg gcgacatatt
     1441 cgggaatctg ttcgaagagg tgttcaacgc cgggggagcg gaaaaagccc ggaggggcag
     1501 ggacctcaaa tactcgctcg acctgacgtt cgaagaatcc atcgacgggt gcgagaaaga
     1561 gctcaggata ccgaggcggg agccctgccg agagtgcgga ggaagcggcg ccgcgagagg
     1621 cggcgaggtc gtctgcacga gctgcggggg gcggggcgag ctcaggtact cgcaggggct
     1681 cttcgccata aagagggcat gcccgacgtg cgggggcacg ggaaagagga ttaccaaacc
     1741 ctgcccgcgc tgcggcggag aaaggttcgt tctttccgag caccaggtga aggtcaaggt
     1801 tccagccggc atctcggacg gtgtcaggct caggatgcgc ggagagggcg acagcggata
     1861 cctcaacggg ccgagcggag acctgtacat agagatacac gtggaggagc atcctctctt
     1921 caggagagag gagcaggacc tttactgcac ggtgccgatc gagttcgttc aggcggctct
     1981 cggggccgag atcgacatac cgacccccaa aggaaagagc accatcaaga taccgcccgg
     2041 aacgcagtcg ggccagacat tcaggctaaa gggaaaggga gtgcccaagc tcgagggcag
     2101 ggggcagggc gatctttata ttcaggtgca ggtggaggta cccgtaaagc tcaacaagaa
     2161 gcagcagcta ctgctcgaag agttcgccag ggcgagcgaa gagggcaacg tcccaaccgt
     2221 gaagaagttc gtatccaggc tccaggaaat actcaacaaa tgacggaagc cgggcgagcc
     2281 tgccccggga ttctgtgact actcgtcaga agccgcctgc tatggcgggg attattgaaa
     2341 ctatgtcccc gtccttaagc tcggtctcct tcccgctgag gaaccttata tcctcgttgt
     2401 tgacgtacag gttgatgaat ctcctgacgt ttccggtctc atcgcacagc ctgctcttta
     2461 tgcccggata ctgggcctcc atctgatcta tgagctccgc gatattcgct gcgtttatcg
     2521 tgacctcgtc cttgtcagat gtcaatttcc taagcggtgt gggaatgcga acagagggca
     2581 tctttcgtac ctcctttatc ttcatgttcc ttgattttct gatacagatc ttcaaactct
     2641 tcgagcctgg cctctattat ctgaggctgg gacacgctgt cggcgattgc ctcctgcgtc
     2701 ttgagcccgt tgcccgtgat agaaaccact atggactcgt ttttgggtat aacaccctgt
     2761 gaaatcagct tctgtgttac ggccatggtc accccgcccg cggtttccgt gaatatacct
     2821 tccgtctcgg caaggag
//
LOCUS       DASSJH010000314         3863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1587632, whole genome shotgun sequence.
ACCESSION   DASSJH010000314 DASSJH010000000
VERSION     DASSJH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587632"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..356)
                     /gene="rplV"
                     /locus_tag="VFJ67_06770"
     CDS             complement(18..356)
                     /gene="rplV"
                     /locus_tag="VFJ67_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET7290128.1"
                     /translation="MVSQAVRKYYRSSPKKLGPVLELIRGNGVENAFTILSALRRRSA
                     PDIAKLLKSAVANAAEKGHTDTESLYVKEAYASKGPTLKRIRPRAMGRAASRKKRMSH
                     ITIVLEERGL"
     gene            complement(369..662)
                     /gene="rpsS"
                     /locus_tag="VFJ67_06775"
     CDS             complement(369..662)
                     /gene="rpsS"
                     /locus_tag="VFJ67_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET7290129.1"
                     /translation="MPRSSKKGPYVSERLQEKVDAERASGSSRIIRTWSRRSMVTPDM
                     IGLTFAVYNGRKFIPVYVTEHMVGHKLGEFSPTRTFTGHSGDKKAKVTKGKSE"
     gene            complement(672..1514)
                     /gene="rplB"
                     /locus_tag="VFJ67_06780"
     CDS             complement(672..1514)
                     /gene="rplB"
                     /locus_tag="VFJ67_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7290130.1"
                     /translation="MGIKKFRPTSAGTRFRSGSDYSDITSDRPHKPLTSPLKKRGGRN
                     AHGRITSFQKGGGNKRLYRAIDFKRDKYDIPAKVESIEYDPNRSSRIALLFYADGEKR
                     YILAPEGLKVGDKVFSGENVEVAPGNALPMRSIPVGTVVHNVELKPKGGGKLARAAGA
                     SAQIVAREGGYAQLKLTSGEIRLVSVECMATVGKIGNSEHELISIGKAGRQRHRGRRP
                     HVRGVAMNPVDHPHGGGEGKASKGNPHPVSPWGWITIGYKTRKNKRTDNFIVKRRRIG
                     YGMD"
     gene            complement(1517..1804)
                     /gene="rplW"
                     /locus_tag="VFJ67_06785"
     CDS             complement(1517..1804)
                     /gene="rplW"
                     /locus_tag="VFJ67_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7290131.1"
                     /translation="MKDPRQIIKRPLVTEKSSDSREKNWYVFSVDKRSNKQEIKDAIE
                     KIFKVKVGKVRTLVTTGKKVKRFGRVVGKKQSLKKAYIEIKEGTIEIFEGV"
     gene            complement(1801..2424)
                     /gene="rplD"
                     /locus_tag="VFJ67_06790"
     CDS             complement(1801..2424)
                     /gene="rplD"
                     /locus_tag="VFJ67_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET7290132.1"
                     /translation="MPQLDVFNLDKNKVGTIDLDSSIFEAPVRQHLLHTVVNWQLTKK
                     RAGTSSTKERGEVSGGGKKPWKQKHLGRARQGSIRAPQWRKGAIIFGPKPKDWSYTLN
                     KKSKKQALISSLSLKYGEGRMLALNEITLPSVKTKQVAEFVKRFELKSALIIIGSDND
                     NLLKSSRNMPNVKVLKIDGINVYDILRYDTLVMTEESLARAQEVLAN"
     gene            complement(2411..3058)
                     /gene="rplC"
                     /locus_tag="VFJ67_06795"
     CDS             complement(2411..3058)
                     /gene="rplC"
                     /locus_tag="VFJ67_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005509762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET7290133.1"
                     /translation="MIEGILGKKLGMTEFFLENGTSVPATVIEAGPCFVVQKRTAEKD
                     GYEALQIGFGEMKPQRVTKPMLGHFKKAGVSPLRHIVEFSAKAGDYNPGDEIRADIFK
                     EGETVDVVGTSKGKGFAGAMKRHHFAGQPASHGGMAHRRPGGIGSNTYPARVWKGLRM
                     PGHMGAERVTHQGAQIFRVDADRNIIIIKGSVPGPRGGLVIVKRTSKGSRESAAA"
     gene            complement(3064..3384)
                     /gene="rpsJ"
                     /locus_tag="VFJ67_06800"
     CDS             complement(3064..3384)
                     /gene="rpsJ"
                     /locus_tag="VFJ67_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET7290134.1"
                     /translation="MNRAAKIRIKLKSFDHRLLDRSSVEIVDTAKRTGAKVAGPVPLP
                     THISRYCVLRSPHVDKNSREHFEVRTHKRLVDILEPTQQTIDALMKLDLASGVEVEIK
                     LTGS"
     gene            complement(3407..>3863)
                     /locus_tag="VFJ67_06805"
     CDS             complement(3407..>3863)
                     /locus_tag="VFJ67_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HET7290135.1"
                     /translation="EIVGFGDTRKTVATSLEMFRKILDDAQAGDNVGVLLRGTGKDEV
                     ERGQVLAKPGSITPHKKFKAEVYVLKKEEGGRHTPFFPGYRPQFYLRTTDVTGSIVLP
                     EGVEMVMPGDNINMDVELIAPVALEDQLRFAIREGGRTVGAGVVTKIVE"
BASE COUNT          676 a         1209 c          907 g         1071 t
ORIGIN      
        1 aaactttctg acccaattta tagccccctc tcttcaagta ctatggtgat atggctcatt
       61 ctcttcttcc tcgaggccgc ccttcccatc gcgcgcgggc gtatcctctt gagcgtcggt
      121 cccttagagg cgtaggcctc cttcacgtag agcgattcgg tatcggtgtg gcccttctcg
      181 gcggcgttcg ccactgccga cttgagcagc ttcgcgatat cgggcgccga cctcctgcgg
      241 agggcggaaa gaatcgtgaa agcgttctcc acaccgttgc cccttataag ctccaaaacc
      301 gggccgagct ttttcggtga tgacctgtag tattttctta ccgcttgcga aaccatcgta
      361 ttcccttttt attcggattt gcctttcgtt actttcgcct tcttgtcgcc ggaatggccg
      421 gtaaacgtcc tcgtcggaga gaattccccg agcttgtggc cgaccatgtg ctcggtaacg
      481 taaacgggga tgaacttgcg cccgttgtag accgcgaacg tgaggccgat catatcggga
      541 gtgaccatag accttctcga ccacgtcctg ataatgcgcg agctgccgga cgccctctcg
      601 gcatccactt tttcctgcag cctctcactt acgtacggtc cttttttgct cgatctcggc
      661 ataacctctc cttaatccat tccgtaaccg attcttctgc gtttcactat gaaattatcg
      721 gttctcttgt tcttccttgt cttgtatccg atcgttatcc agccccaggg tgatactgga
      781 tgagggttgc ccttgctggc cttgccctcg ccgccgccgt gcgggtgatc gaccgggttc
      841 atagcgacgc ctctcacgtg gggacgcctg cccctgtgtc tctgacgccc cgccttacct
      901 atggatatga gctcgtgctc ggagttcccg atcttgccta cagttgccat gcactcgacg
      961 ctaacgagcc ttatctcgcc tgatgtcagc ttcagctggg cgtatccgcc ttccctcgcc
     1021 actatctggg ctgacgcgcc ggccgccctc gcgagctttc cgccgccctt gggtttcagc
     1081 tccacgttgt gaacgaccgt gccgacgggg atgctcctca tgggaagcgc attgcccgga
     1141 gccacttcca cgttttcccc ggagaacacc ttatcgccca ccttgaggcc ttcgggcgcc
     1201 agtatgtacc tcttctcgcc gtccgcgtag aaaagcagcg ctatcctcga cgaacggttg
     1261 gggtcgtatt ctatggactc caccttagct gggatatcgt atttatcgcg cttgaaatcg
     1321 atcgccctgt ataagcgctt gttgcccccg cccttctgaa aactcgtgat ccgcccgtgg
     1381 gcattcctgc cgcctctctt cttaaggggc gacgtaaggg gcttgtgggg cctgtcggag
     1441 gttatatcgg aatagtccga acctgacctg aacctcgtac cggcgcttgt ggggcggaat
     1501 ttcttaatac ccattattaa actccctcaa aaatctctat tgtgccttct ttgatttcaa
     1561 tataagcctt tttcaacgac tgcttcttgc cgactacgcg tccgaacctc tttacctttt
     1621 tcccggtagt gacgagagtc ctgaccttgc cgaccttcac cttgaatatc ttttctatcg
     1681 cgtcctttat ttcctgcttg ttcgacctct tgtcgaccga aaaaacgtac cagttcttct
     1741 ctctgctgtc ggaagatttt tccgttacca acgggcgctt tattatctgt ctcgggtctt
     1801 tcaattcgcc aggacctcct gcgcgcgcgc aagcgattct tcggtcatta caagcgtgtc
     1861 gtacctgagt atgtcgtaca cgtttatgcc gtctatcttg agcaccttca cgttcggcat
     1921 gttcctcgat gacttgagga ggttatcgtt atcgctcccg attattatca atgcggattt
     1981 aagctcgaac ctttttacga attccgcgac ctgtttcgtc tttaccgacg gcagggtgat
     2041 ttcgttaagc gccagcattc tgccctcgcc gtatttaagc gatagcgagc tgatgagcgc
     2101 ctgcttcttg cttttcttgt ttaaggtgta ggaccagtcc ttgggctttg ggccgaatat
     2161 tatcgcgccc ttcctccatt gaggggccct tatgctgccc tgacgagcgc gcccgaggtg
     2221 tttctgcttc cagggcttct ttccgccgcc gctcacctcg cccctctcct tcgtcgaaga
     2281 ggtacccgca cgctttttcg tgagctgcca gtttacgacc gtatggagga ggtgttgcct
     2341 tacaggcgct tcaaatatcg aggaatcgag atcgattgtc cccactttat tcttatccag
     2401 attaaaaacg tcaagctgcg gcactttctc tgctcccttt agaagttctc ttgactatta
     2461 caagaccgcc cctgggaccg gggaccgagc ctttgattat tattatgttc ctgtcggcgt
     2521 ccaccctgaa aatctgagcg ccctgatgtg taaccctctc agcgcccatg tgcccgggca
     2581 tcctgagacc cttccacacc ctcgccgggt atgtgttcga gccaatgcct ccgggcctcc
     2641 tgtgggccat gccgccgtgt gaagcgggct gtcccgcgaa gtggtgtctc ttcatggccc
     2701 ccgcgaagcc tttacccttg cttgtgccga cgacgtcgac cgtctcccct tccttgaata
     2761 tatcggccct tatctcgtct ccggggttat agtctccggc cttcgcgctg aattcgacta
     2821 tatgcctgag cggagaaacc cccgcttttt taaagtgtcc gagcatcggc ttcgtcactc
     2881 tctggggctt catctccccg aagcctatct gtagggcctc gtagccgtcc ttttcagcgg
     2941 tcctcttctg gaccacgaaa cacggaccgg cctcgatcac ggttgcggga acagatgtgc
     3001 cgttctcaag aaaaaattcg gtcattccaa gttttttgcc taatattcct tcaatcatcg
     3061 aaatcacgat cccgttagtt ttatctcaac ctctacgccg gaagccaggt cgagcttcat
     3121 cagcgcatcg atcgtctgtt gcgtaggctc gagaatatcg acgagcctct tgtgagttct
     3181 cacctcgaaa tgctctctcg aattcttgtc gacgtgagga gacctcagca cgcagtagcg
     3241 gctgatatga gtgggcagag gtacgggccc tgcgaccttg gcccctgttc tctttgccgt
     3301 atccactatc tccacagaag acctgtcaag gagcctgtga tcgaacgatt tcagttttat
     3361 acgtatctta gcagcacgat tcatgtatta aattcccttg tcacgctcac tccacgattt
     3421 tagtaactac acccgcaccc actgtccttc cgccttccct gatcgcgaac cggagctgat
     3481 cctcgagcgc cacaggagct atcagctcta cgtccatgtt tatgttatcc ccgggcatca
     3541 ccatctccac tccctcggga agcactatcg accccgttac gtccgttgtc cggaggtaga
     3601 actgaggcct gtaccccggg aagaacggcg tatgcctccc cccctcttcc ttcttcagca
     3661 catacacctc ggccttgaac ttcttgtgag gcgttatgct cccgggcttt gcaagcacct
     3721 gtcccctctc gacctcgtcc ttacccgttc ccctgagtaa cactcccacg ttgtccccgg
     3781 cctgcgcgtc gtccagtatc ttcctgaaca tctcaaggct cgtggccact gtcttacgcg
     3841 tgtccccgaa ccccactatc tct
//
LOCUS       DASSJH010000315         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1045107, whole genome shotgun sequence.
ACCESSION   DASSJH010000315 DASSJH010000000
VERSION     DASSJH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045107"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..619
                     /locus_tag="VFJ67_06810"
     CDS             296..619
                     /locus_tag="VFJ67_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290136.1"
                     /translation="MTEQKKGSIIAGLFWMLVISILLFWIPTIGPLIAGIVGGKKSGG
                     VGNAILAALLPAILMGGLLFLFGSALTGAPIIGFVAGMGAFFLVVSNVGPLLIGAIIG
                     GLLTD"
     gene            complement(768..1949)
                     /locus_tag="VFJ67_06815"
     CDS             complement(768..1949)
                     /locus_tag="VFJ67_06815"
                     /inference="COORDINATES: protein motif:HMM:NF016427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7290137.1"
                     /translation="METKYNEFDETVSVSDYVEFESDGDEVSSINFKDSYSREEGNDN
                     EYGSGKVNSKSKSDDTPDEQLRLLYVYFKDMSVEPLFTAQEEVEISAKIKKCESRSRE
                     LVSLLGKLADGKVKINGVNGHRNGHKRFNNGYMDKRIKSLNAFVRVYSENAKRYKQRF
                     VKANLRLVITISRKYMGRGLPLSDLIQEGNMGLMRAVERFDHRKGFKFSTYASWWIHQ
                     AILRALQGQTRTIKVPVYLLEQANRVYKVNAILAKKLGRKPTPKEIARKSGISVEVVK
                     RILRSTKDAISLDTPILDGEKTTLLDSIADNDTVIPDSLVAKSDLTDKLREALLLLNP
                     REEEILRLRFGIDQHSTYTLDEIGRKFNLTRERIRQIEKAALGKLANSDIKDHLESFL
                     R"
     gene            complement(2224..2835)
                     /locus_tag="VFJ67_06820"
     CDS             complement(2224..2835)
                     /locus_tag="VFJ67_06820"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET7290138.1"
                     /translation="MPYQLPKLPYDYDALEPHIDARTMEIHYTKHHQTYINNVNAALE
                     KYPALAEKTVEDLIKDLNAIPEDIRTAVRNNGGGHANHSLFWTIMGPKAGGEPTGELA
                     DAIKSGFGGFDGFKDQFSKAATGRFGSGWAWLSVDGGGKLVLSSTANQDSPLSDGLTP
                     ILGLDVWEHAYYLNYQNRRPDYIAAWWNVVNWKQVASNYARAK"
BASE COUNT          700 a          848 c          745 g          886 t
ORIGIN      
        1 cggtcttaaa gaagcacttc gtagagacat cggaaaacga tacggaaacc acaaagcgcg
       61 cagttgattt aaatctcgag cgctgtcccg actgcggaga cagggcgctc gcgtttgagc
      121 agggatgcat gacctgcaga agctgcggat tttccaagtg cgactgatag attaaaaacc
      181 ctataagagg atgatgacag gcaaaaagcc cggcccctta atggaggttg ggctttttgt
      241 tttgattgga tattttatat aatgagtatc agatcgttgt acgcggatga acactttgac
      301 tgagcaaaag aaaggaagca ttatcgctgg gctcttctgg atgctcgtca tatccattct
      361 tcttttctgg atacctacca taggcccgct tattgccgga atcgtgggag ggaaaaagtc
      421 tggcggtgta ggaaacgcaa tactagccgc ccttctcccg gcgatcctca tgggcggtct
      481 gcttttctta ttcgggtcgg cccttacagg cgcccccata ataggcttcg tggccgggat
      541 gggcgcgttc tttctcgtag tctcgaacgt gggcccgctc ctcatcgggg cgattatcgg
      601 cgggcttctg actgactaac cttaccagaa tttatcggtt attaacgcta tagccgaatc
      661 caatatgccg gagtagagct ggagggtatt tattcgggca ttacgtctga ttccggggaa
      721 tggaccggag ccgtaatgga atcgtgaggc ttaacagccc cccaggctca cctgagaaaa
      781 ctctcgaggt ggtcttttat atccgagttg gcgagcttgc ccagcgccgc cttttcgatc
      841 tgtcttatcc tctcgcgcgt gagattgaac ttccttccga tctcgtcgag cgtataggtg
      901 ctgtgctgat cgatcccgaa cctgagcctc aatatctcct cctccctcgg attgaggagc
      961 aggagggcct cccttagctt atccgtgagg tcggatttgg ccactaagga gtccggaatt
     1021 accgtatcgt tatccgctat agaatcgagg agtgtagtct tctcgccgtc gagtataggt
     1081 gtgtcgaggc ttattgcgtc cttcgtcgac ctcagaatgc gctttaccac ctccacggat
     1141 ataccggatt tacgggcgat ttccttcggt gtgggctttc tgccgagctt cttcgcgagt
     1201 atggcgttca ccttgtaaac cctgttcgcc tgctcgagca ggtaaacggg gactttaatc
     1261 gtcctggtct gtccctgaag cgccctgagg atagcctggt gtatccacca cgatgcgtac
     1321 gtggaaaatt tgaaaccctt tctgtgatcg aacctttcga cggcccgcat gagccccatg
     1381 ttcccttcct gtatcagatc ggaaaggggg agtcccctgc ccatgtactt tctcgagatc
     1441 gttatgacga gcctaaggtt ggcctttacg aacctctgtt tatacctctt tgcgttttcc
     1501 gaatataccc tgacaaacgc gttcaggctc tttatgcgct tgtccatata tccgttatta
     1561 aatcttttat gtccgttcct gtgaccgtta acgccgttga tcttgacctt tccgtcagcg
     1621 agtttgccta acaggcttac gagctccctc gacctcgact cgcatttctt tatcttcgct
     1681 gaaatctcga cctcttcctg cgccgtgaac agcggctcga ccgacatgtc cttgaagtaa
     1741 acgtaaagca ggcggagctg ctcgtccggg gtatcgtccg attttgactt ggaatttact
     1801 ttgcccgaac cgtactcgtt atcatttccc tcttccctcg aataactgtc tttgaaattt
     1861 atggacgaga cttcgtcacc gtcactctcg aactctacat agtcgctcac ggatacagtt
     1921 tcatcgaact cgttgtattt tgtttccatt tcttcttcct tttaacgata ttcaaccgct
     1981 gaaataaaac cgaagatgct cccggtcatc gtatctcacg aacgaaaacc ggaaacgaaa
     2041 ggggtgggaa attaccattc ctccggatac cgactgaaaa actctgattg cgggactctt
     2101 atacaatcct ctaccgtctg cctgccaatt tcttcaacaa tactgagcct gaactcagcc
     2161 cttaaaaact ttcctgcctt ccggttaccg ccaattctat caaattctaa aaattattgc
     2221 aacctacttt gctcgggcgt aattagaagc cacctgcttc cagttaacca cgttccacca
     2281 cgcagcgatg taatcgggcc tcctgttctg ataattgaga taataggcat gctcccacac
     2341 gtcaaggccc agtatcggcg tcagcccgtc cgagagcggg ctatcctggt tcgcagtcga
     2401 tgacaggact aatttcccgc cgccgtcgac cgacagccat gcccagccgc ttccgaacct
     2461 cccggttgcc gccttcgaga actggtcttt gaagccgtcg aatccaccga agccggactt
     2521 aatggcgtct gcgagctctc ccgtcggctc cccgcctgcc ttcgggccca taatagtcca
     2581 gaagaggctg tggtttgcgt gtcccccgcc gttgttcctt acggccgtcc ttatgtcttc
     2641 gggtatcgca ttcaggtcct taatcaggtc ctcgaccgtc ttctccgcga gcgcggggta
     2701 cttctcgaga gcggcattta cgttatttat gtatgtctgg tggtgcttcg tatagtgtat
     2761 ttccatggta cgggcatcga tatgaggctc caatgcatcg tagtcataag gaagtttggg
     2821 tagctggtaa ggcatggtta ctcccccctg agtcgaattt tttcaaatgg agaaccgata
     2881 ttgcgggtat ttcacattga aaaatcagtt caatgccact gaaatttgat taattatacg
     2941 acggatttgg gatcccgtct taattatcta gtctacctta aatatccgcc cagtcaaagc
     3001 aatttgcccc ctgtaccacg agcatctttt atcccgcccc cgatctgcgg tatattaaat
     3061 ccccggcccc cgggaatccc aaattgacag cctaggctaa cactggtaat ttttaccata
     3121 taataatttg tatattctgg attcgtgata tccataataa ggtataataa ttaagatat
//
LOCUS       DASSJH010000316         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1153603, whole genome shotgun sequence.
ACCESSION   DASSJH010000316 DASSJH010000000
VERSION     DASSJH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153603"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFJ67_06825"
     CDS             <1..697
                     /locus_tag="VFJ67_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HET7290139.1"
                     /translation="VDYKERIIKRIFRFSETTASEIMIPLIHVSALNDEAKVGDAVRM
                     IKETGYSRIPLYSHRVDNITGMLHSFYLLGADPNERVKNFSRQPFYVPESKLVDELLD
                     EMKEGKAGMAVVVDEYGGAVGVIALEDIIEEIVGEIEDEYDKGIKLWRKIGEGEYLVN
                     PKIEIDVINDDLGIGIPESVDYETLSGFLLSEAGSIPKTGDKIKLKNCVFAITRATTR
                     SIDEVKLVITKKK"
     gene            complement(714..1145)
                     /gene="ybeY"
                     /locus_tag="VFJ67_06830"
     CDS             complement(714..1145)
                     /gene="ybeY"
                     /locus_tag="VFJ67_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7290140.1"
                     /translation="MKISISDETGLLGAAYKSALKKIASAVLRGMGLPSTTELSLSFI
                     DDRSMRELNREWRNIDRATDVLSFPQEGGPDYTLLGDIVISLEAAERQSVRYGNTLHE
                     EIKKLIVHGALHLLGHDHKKKKEKEIMREEESRLMLLIKEL"
     gene            1258..1848
                     /gene="hisB"
                     /locus_tag="VFJ67_06835"
     CDS             1258..1848
                     /gene="hisB"
                     /locus_tag="VFJ67_06835"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET7290141.1"
                     /translation="MKQRKAKTERVTSEVKVKVEINLDGKGVYEIDTGIPFFNHMLSQ
                     FAKHGYFDIKISAAGDLDVDFHHTVEDVGLALGDAFLKALGDKKSIRRFGEAFVPFDE
                     TLAFASVDLSGRPYLVYKVRVPKSKVGEFDLELGEEFFKSFSNTLMCNMHIELKYGDN
                     LHHMIEAIFKAAGRALDNATRIDPRSSDVPSTKGKL"
     gene            1852..2499
                     /gene="hisH"
                     /locus_tag="VFJ67_06840"
     CDS             1852..2499
                     /gene="hisH"
                     /locus_tag="VFJ67_06840"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET7290142.1"
                     /translation="MIAIVDYGMGNLRSVSKAFQNQGFDVSVTKDPGEIESASGLVLP
                     GQGAFGDCVRNLEENGIFGTIRGYIQSGKPYLGICLGLQVLFEESEESPGVSGLGLLK
                     GRVVRFPSFKKERLKVPHMGWNRIGIKKDTAVLEGIPDGSWFYFVHSYFPAPEDESVI
                     AITCSYGLEFTAAVATGNMFACQFHPEKSSTYGLRLLRNFGLLCGEKIKNRKQSS"
     gene            complement(2492..>4093)
                     /locus_tag="VFJ67_06845"
     CDS             complement(2492..>4093)
                     /locus_tag="VFJ67_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET7290143.1"
                     /translation="AYEKQGKYSEAVNTYKQAWVEFPETKSSFSAQKAARDISALQGV
                     PFVPTESDYLQRADKLFELSRWSSAAQNYEQVSSQSAEVRTRMGIAMVNAGRLNEAES
                     VLSGISSPESLFWRGKLKSKQGLDSEASALYTQIHTLFPSSELAPEGLYNSGRLYQIN
                     DDYQNAIKTYDLLIRTYPKNSFAEDGAWNLGWIYYKRGMYPEALATFSAFTGSSSAFN
                     AANSRYWKARTLEKQGRKDEAMAEYMLLAGMTTPTYHSYLAQRKTGFTPSYPNFSAES
                     MALNPSAAAKKEKAEILIRLGLPEDARLEIEKMEALAVTQEEHVAVSLLYSKVEDYYN
                     SIKVAQDIGLPQANSLSYPRGYKDIVSSYAKKYGVDELLVYSVIREESRFQKDVVSPA
                     DAVGLMQLIPPTARVVASQIGLGGFTPEMLTIPRINIEMGIFYLREVLDKFDGDVELA
                     LASYNAGPNRAADWKVDFYGLDKDEFVEEIPFRETRNYIRRILRSYGAYKAIYGNSAS
                     AAPRNTTPAQNTAPVPNTATESQVN"
BASE COUNT          878 a         1063 c         1214 g          938 t
ORIGIN      
        1 agttgattac aaggagagga tcataaagcg gatattcagg ttctcggaaa cgaccgcgag
       61 cgagatcatg atacctctca tccacgtgag cgcgcttaac gacgaggcga aggtcgggga
      121 cgccgtgcgc atgatcaaag agaccgggta ttcgaggatc ccgctataca gccaccgcgt
      181 ggataacatc acggggatgc tccactcgtt ctatctccta ggggccgacc cgaacgagag
      241 ggtgaagaac ttctctcgcc agccattcta cgtgcccgag tcgaagctcg tcgatgagct
      301 gctcgacgag atgaaggagg ggaaggccgg gatggcggtc gtcgtggacg agtacggcgg
      361 ggcggtcggc gttatagcgc ttgaggacat aattgaggag atagtcgggg agatcgagga
      421 cgagtacgat aaggggataa agctctggag gaagatcggc gagggagagt atctcgttaa
      481 tccaaagatc gagatagacg tgataaacga cgacctcggg atcggcattc ccgagagcgt
      541 cgactacgag accctcagcg ggttcctcct gtccgaggcg ggctcgatac cgaagaccgg
      601 cgacaagatc aagctcaaga actgcgtgtt tgcgattacg agggcgacca cgcgctcgat
      661 agacgaagta aagctcgtta taaccaagaa aaaataaatt ctctgagttc ctcttacagt
      721 tcttttatca gcagcatgag gcgggactcc tcttcccgca ttatctcttt ttccttcttc
      781 ttcttatggt cgtgcccgag aaggtggagc gcgccatgga cgatgagctt cttgatctct
      841 tcgtgaaggg tgttgccgta ccggacgctc tgtctttcgg ccgcctcgag agagatgact
      901 atatcgccga gaagcgtgta gtcggggcct ccttcctggg ggaacgagag cacgtctgtc
      961 gcgcggtcta tattcctcca ctcccggttg agctccctca tactccggtc gtcgatgaat
     1021 gagagcgaga gctcggtcgt ggaggggagt cccatccccc tgagtacggc tgaagcgatt
     1081 ttcttcaggg ccgatttgta cgcggctccg aggaggcctg tctcgtccga gatcgatatc
     1141 ttcattacgt ctattttact cgcgcccggg tgtgcgcgaa agccggagcc ggcgattcgg
     1201 agcccggggt cccgagttgg acagtctttt aaattctgct atgattctta accatctatg
     1261 aagcagagga aagcgaaaac cgagagggtg acgtccgagg ttaaggtcaa ggtcgagata
     1321 aacctcgacg gcaagggcgt ctatgaaata gataccggga taccgttctt taaccatatg
     1381 ctctcgcagt tcgcgaagca cggctatttc gatataaaaa taagcgccgc aggggacctc
     1441 gacgtggatt tccaccatac ggtcgaggac gtcgggctcg ccctggggga cgcgttcctg
     1501 aaggcgctcg gcgataagaa gtccataaga aggttcggtg aggcgttcgt gccgtttgac
     1561 gagacgctcg ccttcgcgtc agtcgattta agcgggagac cgtatctcgt gtataaggtc
     1621 agagtcccca aaagcaaggt cggggagttc gacctcgagc tcggcgagga attctttaag
     1681 tccttctcca ataccctcat gtgcaacatg cacatagagc ttaaatacgg tgacaacctc
     1741 caccatatga tagaagcgat tttcaaggcg gccgggcggg cgctcgacaa cgccacgagg
     1801 atcgacccca gatcgagcga cgttccttcg acgaaaggga agctctgagc catgatcgcg
     1861 atagtcgatt acggtatggg caatctgagg agcgtgagca aggcgttcca gaaccagggc
     1921 ttcgacgtaa gcgtgacgaa ggacccgggc gaaatagaga gcgcgagcgg gctcgtgctt
     1981 ccgggccagg gggctttcgg cgactgcgtc aggaacctcg aagaaaacgg tatattcggg
     2041 acgatacgtg gatacattca atcggggaaa ccttatctcg gaatatgcct cggcttacag
     2101 gttcttttcg aggagagtga agagtctccg ggcgtctccg ggctcggact gctcaagggc
     2161 agggtggtga ggttcccgtc attcaaaaaa gagcgtttga aggttccgca catgggctgg
     2221 aacaggatcg ggataaaaaa agacacggcc gtgctcgaag gcatccccga cgggagctgg
     2281 ttctatttcg tgcattctta ttttcccgcg cccgaagacg agagcgttat agccataacg
     2341 tgttcgtacg ggctggagtt cacggcggcc gtcgcgacag ggaatatgtt cgcgtgtcag
     2401 ttccacccgg agaagagcag cacctacggg ttgaggctcc tcaggaactt cggtctcctc
     2461 tgcggggaaa agataaagaa ccgcaaacaa tctagttgac ctgactttcc gtcgcggtat
     2521 tcgggacggg ggcggtgttc tgagcggggg ttgtattccg gggcgcggcg gaagcggaat
     2581 tcccgtaaat agccttatac gctccgtagc tcctcaatat tctcctgatg tagttcctcg
     2641 tttccctgaa cggaatctct tcgacgaact cgtccttgtc gagcccgtag aaatcgacct
     2701 tccagtcggc cgccctgttg ggcccggcgt tgtaactggc gagcgcgagc tcgacgtccc
     2761 cgtcaaattt gtcgaggacc tctctcagat agaatatgcc catctcaata ttgatcctcg
     2821 gtatggtgag catctcgggc gtgaaaccgc cgagtcctat ctggctcgcc acgactctag
     2881 ccgtaggcgg tataagctgc atcaggccga ccgcgtccgc gggggagacg acgtctttct
     2941 ggaacctgct ctcctccctg atgaccgagt atacgagcag ctcgtcgaca ccgtattttt
     3001 tcgcgtaaga tgagactatg tccttataac cccgggggta ggaaaggctg tttgcctgag
     3061 gcagaccgat gtcctgagcg acctttatgg aattgtagta atcctccacc ttggaataaa
     3121 gaaggcttac ggcgacgtgt tcctcctgag tcacggcgag ggcttccatc ttttcgatct
     3181 cgagccttgc gtcctcaggc aagccgagcc tgatgaggat ctccgccttt tcctttttcg
     3241 ccgcggcgga agggttaaga gccatcgatt cagcgctgaa attcggataa gaaggagtaa
     3301 atccggtttt cctctgcgca aggtatgagt gataggtggg ggtcgtcatt ccggcgagca
     3361 gcatgtattc ggccattgcc tcgtctttcc gtccctgctt ttcgagagtc ctcgccttcc
     3421 agtatcttga gttagccgcg ttgaatgcgg aggatgagcc ggtgaatgca gagaacgttg
     3481 caagggcttc cggatacatc cctcttttgt aatatatcca gccgaggttc cacgctccgt
     3541 cttcggcgaa gctgtttttc gggtacgtcc tgatgaggag gtcgtaagtt tttattgcgt
     3601 tctggtagtc gtcgtttatc tggtagagcc tgccggagtt gtagagtccc tccggggcga
     3661 gctcgctcga agggaataac gtatggatct gtgtgtatag cgcggaggct tccgaatcga
     3721 ggccctgttt cgatttcagc ttccccctcc agaataacga ctccggggag ctgatgccgc
     3781 tcagaacgct ttccgcttcg ttgagcctgc cggcattcac catcgctatg cccatcctcg
     3841 ttctcacctc tgcgctttga gaggagacct gctcgtagtt ctgcgcggct gacgaccacc
     3901 tggaaagctc gaacagcttg tccgcccgct ggagatagtc ggactcggtc gggacgaacg
     3961 ggacgccctg taaggcgctt atatcgcgcg ccgctttctg ggcactgaag gaggacttgg
     4021 tttcggggaa ttcgacccat gcctgcttat aggtgttcac ggcttctgaa tacttcccct
     4081 gtttctcgta ggc
//
LOCUS       DASSJH010000317         9725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1483618, whole genome shotgun sequence.
ACCESSION   DASSJH010000317 DASSJH010000000
VERSION     DASSJH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483618"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..2361
                     /locus_tag="VFJ67_06850"
     CDS             397..2361
                     /locus_tag="VFJ67_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HET7290144.1"
                     /translation="MGSSSSFGAFADLSLLPLIGPGINIDLGPISTASGIAPPDYSDA
                     DTVLSTGLSTGQTGNILSTGVLATNASSDIANDNTSADATVDGLSLSVVGALPLLTIG
                     ADTVVSTADVSGDCGAGSLTATGATTIENGSAGGTLGLGLSIASNPSPNFELINLLGI
                     RVVLNEQIVTGDGVTSRGITVNAIHVDLTNTILSLIGALSGDIIIAQSQAQVQCNEIQ
                     ESAGLSITKSDSPDPVTVGNVLTYTLTVTNSGPDQATGVTVTDVIPSSFTFNNATPSQ
                     GTCNPLVGQTLTCNLGTIANGVNATVTISVTPTQAGVITNTAVVSGNEPDPDPDDNSD
                     TESTTVNGTGPASANLSVVKTDNPDPVIVNQQLTYTVTVTNLGPDAATNVVVTDTLPA
                     GATFGSATPEQGSCNQALGVVTCNLGGISSGSSVNITIIVTPTNVGTAVNTVSVTGDE
                     NDPDTNNNSDTENTAVDPVPQNADLSIVKSDSPDPVIVNQQLTYTVTVTNTGPDTATN
                     VVVTDTLPAGSVFGSATAEQGSCGEAAGVVTCNLSNIANGDSVIITIVVTPTVVGTAV
                     NSASVVSDVNDPDPGDDSDDEDTEVDPSPTGPPPTITPSDPPGSPVPGPRINAIPTLS
                     GWASLAMPILLVLISSAVLRRRVKKNSGSA"
     gene            complement(2577..3125)
                     /locus_tag="VFJ67_06855"
     CDS             complement(2577..3125)
                     /locus_tag="VFJ67_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7290145.1"
                     /translation="MSEKSNFLKKISRYFIPGALGMFIAYGIGTGYMSTASSGSELDT
                     GIHESAAVTDPGPTGLYRPELKDVFFDRNSSRLRSDAKPVLDENAQVLRDEPAMFVVV
                     ETYCDSREESPARLGTERGDAVRDYMNSRGVDAGRVLVVNKCNAYDMELVNSRDTVRL
                     DSRVHFVPLDESLDRVSFAFSN"
     gene            complement(3321..3881)
                     /locus_tag="VFJ67_06860"
     CDS             complement(3321..3881)
                     /locus_tag="VFJ67_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7290146.1"
                     /translation="MRIPIYYILSLSCLLAGCVAGPGSIQDLNNRQAELENKVDAISR
                     DLTDIQRQLEIQTARIKNLVENYSEMYLKLDDLQKKQTVTKPGGTVSKDITKPAPTGD
                     LYQRANTLYNEGQYLNAILAYQVFIDTYPNDGKVADAYLKQGLSLVKIGRPDGAKFFF
                     KTLIDRFPDSREAALAKQQLEKINAD"
     gene            complement(4148..4807)
                     /locus_tag="VFJ67_06865"
     CDS             complement(4148..4807)
                     /locus_tag="VFJ67_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7290147.1"
                     /translation="MNRFLLSSIIATGLALGTSGVFAADQYGNSSDEIRQSENYRQNS
                     SDENLGSEGGEYTVQSGDTLSSIAEAELGSQDRWQEIARANDIENPDRIFAGQKLTIP
                     AANENAGQERSAAEAPAKQNEASNTDMGAKSGAEEGTMSKARNEQSMDQTQQELKGEI
                     TEIGSEKDRFSVKDERGMTHSFTKIEDKDMLEEVSIGDFVKLEIENGTVVSLEKVDRS
                     A"
     gene            5130..6398
                     /locus_tag="VFJ67_06870"
     CDS             5130..6398
                     /locus_tag="VFJ67_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7290148.1"
                     /translation="MKLSKQIRLGFGLMMVLMIVLGTLFVYNVYRLNEAASNIEGRYS
                     TLYKLFSNPHNGVESDPGLGREIVDRAVALVDEQLKYNYTYVFIIVGVSILFAGMITI
                     LFPSKITRPIERLINATKQVKRGDYSYRIKSTGEADEISALAGSFNEMLQNIEDTHRS
                     NLELLEQTKGFNETLKERVEEATSAIREQQSELIRAERLATIGQFAAHIAHEIKNPLS
                     GITVALELMKNKSEDNEQQQSISDVLKEVKRLDRILKDLLQLSIPKEMDFRPADPNDI
                     VERSVVIVSSAADEKGVTIGTNLMCTGEYVLDYEKCQQVVINLLINGIDAVYANEGKV
                     TVETENVNGDVHIRVSDTGRGIPPEEMEKVFEPFYTSKKQGTGLGLAISKKIVEAHDG
                     RITVSSELGKGTTFTVVLPASAAERARITV"
     gene            6459..7835
                     /locus_tag="VFJ67_06875"
     CDS             6459..7835
                     /locus_tag="VFJ67_06875"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7290149.1"
                     /translation="MSQIVLVIDDEEFLCKKLSESLSEEGFLVGTANDGETGLLLARR
                     ENPDLILLDLKLPDMDGIEALSQINNMDPRPVTILMSGHGNVDMAVSAIKMGAYDFIE
                     KPFSLDKLKIIIRNALDNVGLKRNLDTRLRSEQKKYGFHSLIGKSEPIKRIIELFEKL
                     VTTDPKTILISGESGTGKGLAAQVLHHNGLRAQKPIIEINCAAIPETLLESELFGHEA
                     GSFTDAKKMKTGIFEDANGGTIFLDEIGDMSLSLQAKVVKAVEERTFRRIGGKRDIKV
                     DVRVVAATNKDLKELVEKNHFREDLYHRLNVINFEMPSLRERREDIPLLTEHFINYFN
                     MDFNKNIKHVPEEVEKAFMSYQWPGNVRELRSTIERAVLLSEGDTLNPKYVQIDEQFK
                     GVKIQGDEHKMFFELDMDKLTLDTVEEMVIKKALELTNWNQTRAAEMLGVTRQVLRNR
                     MIKMDLLN"
     gene            8070..8339
                     /locus_tag="VFJ67_06880"
     CDS             8070..8339
                     /locus_tag="VFJ67_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7290150.1"
                     /translation="MGFTDIISWIVFGLAAGAVAKLLMPGKDPGGFVGTIVIGIVGAL
                     VGGYGAKILSIGQAVKPGFDLKSFLFAVGGAIILLIVYRFVKGKG"
     gene            8581..9051
                     /locus_tag="VFJ67_06885"
     CDS             8581..9051
                     /locus_tag="VFJ67_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET7290151.1"
                     /translation="MPKLNKIVAFLDEFLGTDDVDDSSWNVLQFEGKSIVKRAAFAVD
                     AGIDAFKMSADVKADILIVHHGHFWDFRNPSITGWAKERIKILFENDISIYVSHNPLD
                     RHKEVGHNAQILKLLGARITDEFFSLSREKHRLDGRVPECVSVEKKLWKSSTGS"
     gene            9027..9341
                     /locus_tag="VFJ67_06890"
     CDS             9027..9341
                     /locus_tag="VFJ67_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET7290152.1"
                     /translation="MEKLNRELKTECRVLPFGRDKVRTIAVCSGGGSYSGFYSALDAG
                     VDLYITGDAVEIYHTAKDAGVNVIFAGHHATETLGLHALSRILQKKFGIETTYIDLPT
                     GL"
BASE COUNT         2475 a         2316 c         2648 g         2286 t
ORIGIN      
        1 aaaacagcag ttttaatctc cttttctatt ttacatatct tttctttttt tactcgcgcg
       61 gaaggattcg tcgaacccgg acggcagggt tactgcggga tattcgataa gtcgagtaaa
      121 aaacgggaga gctattatat attcatgtaa cttcggaggg aactcatgag attttgtacc
      181 aaaaatttaa tcaaggcgtt acaaaatgac gtaatgtgca ttaaaattag cactaaagta
      241 taaaagggga gattttcaga aaaaatttaa aggaggctaa aggtgaattt gttgagagat
      301 cagggagggg cgtatcttct acgattagct catcttgtta tcgcgctgat tgttgtcggt
      361 ttggtttcat taccctcagt tgcgagagct caaacgatgg gcagcagctc ttcgttcggg
      421 gcgttcgccg acctctcgct gctgccgtta atagggccgg ggataaatat cgacctcggg
      481 cccatatcca ccgcttcggg gatagcgcca cccgattatt ccgacgccga cactgtcctg
      541 agtaccgggt tatcaacggg acagaccgga aatattctat cgacgggcgt gctcgcgacg
      601 aacgcaagct cggatatcgc aaatgacaac acttccgccg atgccactgt ggacggcctg
      661 agtctctccg tagtgggggc gttaccgctt ctcactatcg gggcggacac cgtcgtttcg
      721 actgcggatg tgagcggtga ctgcggagca gggagcctga cggcgacagg cgcgacgact
      781 atagagaacg ggtccgcagg cggcacgctc ggactcggtc taagcatagc ttcaaaccca
      841 tcgcccaact ttgagctgat taacctgctc gggatcaggg tcgtgttaaa cgagcagata
      901 gtaacgggtg acggtgttac cagcagaggc ataactgtaa acgcaataca cgtcgatctg
      961 acgaatacta tactttcact gataggcgcc ctgagcggcg atatcatcat tgcacaatcc
     1021 caggcgcagg tgcagtgtaa cgaaatacag gagtcggcgg gcttgtccat cacgaagagc
     1081 gacagccctg acccggtgac ggtgggtaac gttcttacgt acactctgac agtgacgaac
     1141 agcgggcccg accaggcaac cggagttacc gtcaccgatg taataccttc gagctttacg
     1201 ttcaataacg ctacccccag tcaggggacg tgcaacccgc tggtcggcca gaccctgacc
     1261 tgcaatctgg gaaccatagc caacggcgtt aacgccaccg taaccatatc cgtgacaccc
     1321 acgcaggcgg gtgttatcac gaatacggcc gttgtgtcgg ggaacgagcc cgaccccgac
     1381 cctgacgata attcggatac ggaatccacg acagtcaatg ggaccggccc cgcgagcgcc
     1441 aatctttcgg tcgtcaaaac ggacaaccct gatccggtta tcgtcaatca gcagctgacg
     1501 tacacggtaa ccgtcacgaa tctgggtccc gacgccgcaa cgaacgtcgt cgtgacggac
     1561 actctcccgg cgggcgcgac ttttggctcc gccacaccgg aacagggaag ttgtaatcaa
     1621 gcccttggag tggttacatg caacctgggc ggcatttcga gcggcagcag tgtgaatata
     1681 acgattatcg ttactccaac gaatgtcgga acggccgtca acacggtctc cgtcacgggg
     1741 gatgagaacg accctgacac caataacaat tcggatacgg aaaatacagc tgtcgatccc
     1801 gtgccgcaga acgccgatct ttcgatcgta aagtcggaca gccctgaccc ggtgatcgta
     1861 aatcagcagc tcacgtacac agtgacggtt acgaatactg gtcccgacac tgcgacgaac
     1921 gtcgtagtca cggatacgct gcccgcgggg tctgtattcg gctcggctac ggctgagcag
     1981 gggagctgcg gcgaagcagc gggagtagta acgtgcaacc tgagcaacat agcgaacggg
     2041 gacagcgtaa taattacgat agtagttact ccgacggtgg taggtacagc cgtgaacagc
     2101 gcctccgtgg taagtgatgt aaacgaccct gacccgggcg acgactcgga tgacgaagat
     2161 acggaagtcg atccgtcgcc cacgggaccc ccgccgacaa ttactccgtc agaccccccg
     2221 ggatcgcccg ttccgggtcc gaggataaac gcgataccga cactgtcggg ttgggcgtct
     2281 ttagcaatgc cgattctcct ggttctgatt tcgagcgcgg tcctaaggag acgggtaaag
     2341 aagaattccg gaagcgcata acgcgtttga ataaaacggg gagcttatgc catatcatgg
     2401 cataagctcc ttgtaattca gttttcattc ttcatcgtgt atatccgtgc acggatagag
     2461 tggatgataa aaaaagccgt tgtgcataaa agcacaacgg ctttttcgca taatcgggat
     2521 gtttggatgc tcaatccgat ttgtatatat ggaaaaagcc ttcatcccgt ttgttgtcag
     2581 ttggaaaaag caaagctgac tctgtcgaga gactcgtcga ggggcacgaa atgcaccctg
     2641 ctgtcgagcc tgacggtatc cctgctgttg acgagctcca tatcgtatgc gttgcactta
     2701 ttaactacaa gcactctccc ggcgtccacg ccgcggctgt tcatataatc ccttacggca
     2761 tcgcctctct ctgttcccag ccgggcgggg gattcctcgc gggagtcgca gtaagtttct
     2821 accaccacaa acatagcggg ctcgtccctg aggacctggg cgttttcgtc gagtaccggc
     2881 ttcgcgtcag accttaaacg ggagctgttg cggtcgaaga aaacgtcttt cagctctggc
     2941 ctgtagaggc cggtgggacc ggggtcggtt acggccgcgc tctcgtgtat tcctgtatcg
     3001 agctcactcc cgctcgaggc cgtactcatg tagcctgtcc ctatcccgta tgcaatgaac
     3061 atgccgaggg ctccgggtat gaaatatctg gagatttttt ttaaaaaatt agatttttca
     3121 ctcatatcgg taacctcctc aatgtcgaaa ttgatactcg atagtaagaa gtgcaatccc
     3181 cgtgccatgc ggggcaaggg aaataactgt gtgatcagag catattacga gatatgtagt
     3241 gggtcaattg gcacaaatcg tcccgagatg tacggattcg tacaggtgcg ggtgtccggt
     3301 aaagtaccgg aggccgtgtc tcagtcggcg ttaatttttt cgagctgctg cttagcgaga
     3361 gcggcttctc tcgaatcggg gaatctgtct atgagggttt tgaaaaagaa tttcgctccg
     3421 tcgggcctgc cgatcttaac tagagagaga ccctgtttca gataagcgtc cgcgaccttg
     3481 ccgtcattgg gataagtgtc tataaaaacc tgataggcca gtatggcgtt taagtactga
     3541 ccttcgttgt aaagcgtgtt tgcgcgctgg tatagatcgc cggtgggagc tggctttgtg
     3601 atatcctttg agacggttcc cccgggtttg gttacggtct gcttcttttg cagatcgtcc
     3661 agtttcaggt acatctccga atagttctct acgaggttct ttattcttgc cgtttgaatt
     3721 tctagttgtc tctgtatgtc ggttagatca cgcgaaatcg catcgacctt gttttccagc
     3781 tccgcctgcc tgttgttgag gtcctggatg gatccaggcc cggcgacgca accggcgaga
     3841 aggcacgata aggacaatat gtagtaaatc ggtatgcgca tgaccgaccc tggtgtcata
     3901 caatataaat gccggagaga cggataggca gcctcacccg gctccgagta gagaattatt
     3961 tgacattaaa cgcatattgt caaagggtta ggaataatcg gttgaaggta attttttgcc
     4021 tagatattcg gaggaaaaaa gaagaaaaag agaagcaggg acggtccgac cgtccctgcc
     4081 gacggattaa cttaaatagc ggctaccctg cagctgctat ggaagttatg gtgtgagagt
     4141 cttcgagtca ggcgctcctg tcgactttct ccagggagac gactgttccg ttttcgattt
     4201 caagctttac gaagtcgcct atcgagactt cttcgagcat gtccttgtct tctatttttg
     4261 tgaaagaatg agtcatgccc cgctcgtcct taacggagaa tctgtccttt tcggagccga
     4321 tttccgtgat ttcacccttg agctcctgct gcgtctgatc catggactgc tcattccggg
     4381 ctttgctcat cgttccctct tctgcgccgg atttggcgcc catgtcggta ttagaggctt
     4441 cgttctgctt tgccggggct tcggctgcgg atctttcctg cccggcgttc tcattagctg
     4501 ccggtatggt gagcttctgt cccgcaaata tgcggtccgg gttttctatg tcgttggctc
     4561 gcgctatttc ctgccatctg tcttgtgacc ctagctccgc ttccgctatg gatgaaagcg
     4621 tgtctccgga ctgtaccgtg tattctccgc cttccgagcc gagattctca tccgacgaat
     4681 tctgccggta attctccgac tgccggattt cgtcgcttga atttccgtac tggtcggccg
     4741 cgaatacccc gctcgttccg agagcgagcc ctgttgctat aatagatgaa agtaaaaaac
     4801 gattcatgct taatacctcc ctggtattag gatttaattt actcgttgat tattaatcgc
     4861 aagcaccatg ccaacggcat cggctgcggt acataatcaa tgaacgcagg tagttacata
     4921 tatcgccgga cgatcgaaaa gaagggcttt gtatggttta gttgaatatc atacatatat
     4981 tgctgtatca aaccggacaa tgccggataa ttacctgaat gtagagttat ttttaatttc
     5041 acctcttgaa acacacttaa ttgacgggtg gaaaaacagg tagaattatc gtttgcattc
     5101 ttgaatgcaa ggatcgggag atagcacgat tgaagctcag caaacagata agactcgggt
     5161 tcggccttat gatggtcctc atgatcgttc tggggaccct tttcgtatat aacgtttaca
     5221 ggctgaacga ggccgcttcc aatatcgagg gacgttattc gacactctat aaattgtttt
     5281 cgaacccgca caacggcgtt gaatccgacc cggggctggg cagggagatc gtcgaccgcg
     5341 cagttgcgct tgtggatgaa cagctcaaat ataattacac atacgtattt ataatagtcg
     5401 gcgtgtccat tcttttcgcc ggaatgatca caatactgtt cccttccaag atcacgagac
     5461 ctatcgaacg gttgatcaac gcgaccaaac aagtgaagag gggggattac tcctacagga
     5521 tcaagagcac cggggaagcg gacgagatat cggcgctcgc cggctccttc aacgagatgc
     5581 ttcagaacat cgaagatacg cacaggagca acctggagct gctcgaacag acgaaagggt
     5641 tcaatgaaac cctgaaagaa cgggtggaag aggcgaccag cgctatcaga gagcagcaga
     5701 gcgagctgat cagggccgag cggcttgcca cgatcgggca attcgccgcg cacatagctc
     5761 acgagataaa gaacccgctc tcggggataa ccgtcgcact ggagttaatg aaaaacaagt
     5821 cggaagacaa cgagcagcag caatcgattt ccgatgtact gaaggaggta aaaaggctcg
     5881 accgcatact caaagacctg cttcagcttt cgatcccgaa ggaaatggac tttaggccgg
     5941 ccgaccctaa cgatatagtg gagaggtcgg tagttatcgt atcttcagcc gcggacgaaa
     6001 agggagtgac tatcgggact aatctcatgt gtacgggcga gtatgtgctc gattacgaga
     6061 agtgccagca ggttgtcatc aatcttttga taaacggcat agacgccgtg tatgcgaacg
     6121 aaggcaaggt gactgtcgag accgagaacg tcaacgggga tgtccacatc agggtctccg
     6181 acacgggccg ggggataccg cctgaggaaa tggaaaaagt attcgaaccg ttttatacta
     6241 gcaagaagca ggggacggga ctcggactcg cgatatccaa gaaaattgta gaagcgcacg
     6301 acgggagaat taccgtttcg agcgaattgg ggaaggggac gacttttacg gtcgtattgc
     6361 ccgccagcgc cgctgaaagg gccaggatca ccgtataagg tttcacttaa gcgatccccg
     6421 gaagtttcgc aggagcatcc taaagagagg agtcaatagt gagtcagatc gtattggtaa
     6481 tagatgacga ggaattcctt tgtaagaaat tgagcgagag cctgtcggaa gaagggtttc
     6541 tcgtcggcac tgccaatgac ggagagacgg ggctccttct tgcgaggaga gagaacccgg
     6601 acctcatcct tttagacctt aagctcccgg atatggacgg tatcgaagct ttaagccaga
     6661 taaacaacat ggaccccagg ccggtgacga tactcatgag cgggcacggc aacgtggaca
     6721 tggcggtctc ggcaatcaag atgggcgcat acgattttat cgagaaacct ttttctctgg
     6781 ataaactcaa aataatcata agaaacgccc tcgataacgt cgggctcaag agaaacctag
     6841 atacgaggct caggagcgag cagaaaaagt acgggttcca ttcactcata gggaagagcg
     6901 agcccataaa gaggatcata gagctcttcg agaaactcgt tacgaccgat cccaagacta
     6961 ttctgataag cggggagagc gggaccggca aggggctcgc cgcacaggtg ctccaccaca
     7021 acggactcag ggcccaaaag cctattattg aaatcaactg cgcggccata ccggagacgc
     7081 tcctcgagag cgagctcttc ggacacgaag cgggttcttt cacagacgcg aaaaaaatga
     7141 agaccggaat attcgaggac gcgaacggag ggacgatttt tctcgacgag atcggcgaca
     7201 tgagcctctc ccttcaggcg aaggtggtaa aggccgtcga agagcggacg ttcaggagaa
     7261 taggcggtaa gagggatatc aaggtcgatg taagggtcgt cgccgcgacg aacaaggacc
     7321 tcaaggaact ggtcgaaaag aatcatttca gggaggactt gtaccacagg ctcaatgtta
     7381 tcaatttcga aatgccgagc ctgagggagc ggagggagga catcccgctt ctcacggagc
     7441 attttataaa ttacttcaat atggatttta acaagaacat caagcacgtg ccggaagaag
     7501 tcgaaaaggc gttcatgagc taccagtggc cggggaacgt gagggagctg aggagcacga
     7561 tagagagggc ggtccttctc agcgagggcg acactctcaa tccgaaatat gtgcagatag
     7621 acgagcagtt taagggcgtc aaaatccagg gggacgagca caagatgttt tttgagctcg
     7681 atatggacaa gctcacgctc gatacggtcg aggagatggt tataaagaaa gcgctcgaac
     7741 tcaccaactg gaaccagacc agggccgcag agatgctggg cgtaacgagg caggtgctta
     7801 ggaataggat gataaagatg gacctgctga attgaatgtc agatctctat aattcccaaa
     7861 tacaccgata ggtgtccaat agttcaccgg aagttcacca gatttcgtca atacggcatt
     7921 tggctgatcg ttttccttta tttaaggaaa gaatcagaag agaagattgc cccacgaggt
     7981 gcactttgag cttttaggaa aaaattgtaa tatgtcgttt tatagctccg ttattgtaac
     8041 ggataaaacc cgcacgaagg agggatgaca tgggatttac ggacattatt tcctggattg
     8101 tattcggact tgccgcggga gccgtggcaa agctgctcat gcccggaaag gaccccgggg
     8161 gattcgtcgg gactatcgtc atcggtatcg tcggagcgct ggtcggaggg tacggagcaa
     8221 agatactttc tatagggcag gcggttaagc ccgggttcga tctaaagagc tttttattcg
     8281 cggtaggggg ggcgattata ttgctgattg tgtaccgctt cgtgaagggg aaaggttagg
     8341 agggtttggt tcggggcatt cattctgtct tcataacggt gtgccgtttc gaacatccga
     8401 cttccccctt cgagcttcga aggacgggaa tttttgtttg aatgagtgaa atgtgacacc
     8461 cccgtactct ccttccccct ctaaggggaa ggaattctga atgagggaag ggaatatcaa
     8521 aggcgtgcct cggagatttt agctgttttt acgcataata gaatatttct cgctcaatca
     8581 atgccaaagc tgaacaagat cgtcgcattt cttgatgagt ttctcggaac cgatgacgtg
     8641 gatgattcct cgtggaacgt gctccagttt gaagggaaaa gtatcgtgaa gagggcggca
     8701 ttcgccgtcg acgccgggat tgacgctttc aaaatgtccg cagatgttaa ggcggacatc
     8761 ctgatcgttc accacggcca tttctgggat ttccggaacc cttcaattac cgggtgggcg
     8821 aaggaaagga tcaaaatttt gttcgagaat gatatttcca tttatgtaag tcacaacccg
     8881 ctcgaccgac ataaagaggt cgggcataac gcacagatac tcaaacttct gggcgccagg
     8941 attacagacg agtttttttc actatcacgg gaaaaacatc ggctggacgg gagagttccg
     9001 gaatgcgttt ccgttgaaaa aaaattgtgg aaaagctcaa ccgggagtta aaaaccgaat
     9061 gcagggtgct cccattcggg agggataagg tgaggacgat cgccgtctgc agcgggggag
     9121 ggagctacag cggcttttac agcgcactcg acgcaggcgt cgatctctac atcacggggg
     9181 acgcggtcga gatataccac acggcgaagg acgccggggt aaacgtgata ttcgccgggc
     9241 accacgcgac cgagacgctg ggtttacacg ccctttcccg tatcctccaa aagaagttcg
     9301 ggatagaaac cacttacatc gaccttccga cgggattata gactattcca ctgccctcgg
     9361 cgctatagaa gtcaagtaat tcgaggacag aagttatcct atcggaatac atatataagc
     9421 gttaattttt cctcttttcc ttgatgtaca cgtagtgttt tctatgaaag ggcttgagct
     9481 ttctactttt gagagatcaa aagtagcaaa aatcttttgg ggactacggg aagtttgcta
     9541 aaaatcttgt gtttaagcga aaatctttca aatggaaagc tgaaagattt ttttacgctt
     9601 aaacacgcga ttttctttac gcaaatcccg taatgtccct attgcccgct gttttgcagc
     9661 gggcaatatt ggtgttgggc cggagattct gcgaatgtcg gaaagataaa aatggtgccg
     9721 aagcg
//
LOCUS       DASSJH010000318         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_185545, whole genome shotgun sequence.
ACCESSION   DASSJH010000318 DASSJH010000000
VERSION     DASSJH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185545"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..628)
                     /locus_tag="VFJ67_06895"
     CDS             complement(125..628)
                     /locus_tag="VFJ67_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290153.1"
                     /translation="MPLFEYECDKCMDRYNRDIDALVAKLNKTNAARIRKENPGFVYI
                     EVTDGDKDKQIFSLGERSGSRSARRFRYKLDNNKILFLELKNFRFSSLVYEKEEEKDI
                     TCPLCGEKGVRRVFSTFKAIFDDKSKRAPGRGDELRWHLEYKQQKDEEIKNDWVGQEH
                     LDQYFNR"
     gene            782..1573
                     /locus_tag="VFJ67_06900"
     CDS             782..1573
                     /locus_tag="VFJ67_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7290154.1"
                     /translation="MPDKNELIMEEKGAVCSIVFNRPEKRNLLTPGMLLGLEKALGRL
                     GKDGRTRCVVIRGAGDKAFSSGYDISSIGKDDMIKNHSGENPLPRAVKSIENFPYPVI
                     AMLNGHAFGAGLEIAATCDIRTAAKGCLLGMPPAKLGVIYSYRGVRKFLNLIGPGYTK
                     ELFLTGRPVDSERAERMGLVNVTLSAEVLEKFTYDMAGEITENAPLSLKGLKEMVNVW
                     QRNQVIHGEDEELLKKLTLSVQESEDYKEGQRSFAEKRKPIFKGE"
     gene            complement(1598..3067)
                     /locus_tag="VFJ67_06905"
     CDS             complement(1598..3067)
                     /locus_tag="VFJ67_06905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7290155.1"
                     /translation="MKKELFSGFLKENYLIILIVILGAVLRFYGLGREGIWYDEAVSI
                     SVSKLGFKDIIKWVFDNTAETNPPFYYMILSLWTAMFGDSEAVSRIPSAIFGSVSVIA
                     IYAVGKLLYNKRTGLIAALILAVSFFHIKYAQEARGYTLMVCLILVSYYSFFRLTIRR
                     STLYAAVYVVSTALLVYTHYYGTMIILAQNIFCFTLLLRKRKIGEIGLGGWIKLQALT
                     GLVLLPGMVFLAVIALKIQKGFWVPEPSSEAIWQFFRIYAGSTYLLIVFLVFSLYSFA
                     GLRIGKSANVKKSGNISTESAPAAGLSEGEKIYILLVWMAVPVLVPYLISLVSSPILI
                     FRYTIGASLAFYLLASKGIANMNNRWPIAAAAGLILILSSYNIAHYFTTAGKHQWREL
                     MAYVESDAGLGDIIIVSPVYERVTAEYYHKRKDLRIVPLGKKFPTFENLGGRSIWFVF
                     HAHPESRANTRAGLGGKYDITEMSFNRLDLFRLSKKQNN"
     gene            complement(3071..>3710)
                     /gene="era"
                     /locus_tag="VFJ67_06910"
     CDS             complement(3071..>3710)
                     /gene="era"
                     /locus_tag="VFJ67_06910"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET7290156.1"
                     /translation="EEADVILMMIEVRDPFGKGDRFIIENLPKPAILIINKIDTVKKG
                     DLLRIIDESRRFEGRFLEVIPLSARNADGIGELLDTVVGYLPEGPKYFPEDTITDQPE
                     RFLAAEFIREKIFQLTREEIPYKSAVVIEEFKDVPEKGLVRISAVIYVERMNHKGIII
                     GKKGEMLKMIGSLAREDIEKVLGAKVFLELWVKVSERWTERENLIKDYGYGI"
BASE COUNT          895 a          989 c          912 g          914 t
ORIGIN      
        1 ctccgaaacc aattttttcg taaccccttt cttgatagcc ttcaaggagt cttccacgtt
       61 ctttctgcac tgtatgcatt catactcgta aagcggcatt aattctatct ccctcggtta
      121 tttattatct gttgaaatac tgatccaggt gttcctggcc gacccagtcg ttcttgattt
      181 cctcgtcttt ctgctgttta tactcgagat gccatctgag ctcgtctccg cgaccgggag
      241 ccctcttgct cttatcatcg aaaatggcct tgaacgttga gaataccctg cgtacaccct
      301 tctcgccgca gaggggacac gtaatgtcct tttcttcttc cttctcgtaa acgagcgagc
      361 tgaatctgaa gttcttgagc tcgaggaaga gtattttatt gttatcgagc ttgtagcgga
      421 accttctggc gctcctgctg ccgctccttt cacccaggga aaagatctgc ttgtctttat
      481 ccccgtcggt gacctctata tagacgaagc ccgggttttc cttccttatc ctggccgcgt
      541 tcgtcttatt gagtttggcg accagagcat ctatatccct gttgtacctg tccatgcact
      601 tgtcacactc gtattcgaat aatggcattt tatgactccc agttcttaat aagttactaa
      661 gaataaccaa atttttcaaa ttgctccagc cctgcacctc aggcgggcat gaaatttgac
      721 aaagcatccc ccgtgtcgat actagaatat aaatcattta tacccggccc gggaggaccc
      781 catgcctgat aagaatgagc tcataatgga ggaaaaggga gctgtctgca gcatagtttt
      841 caacaggccc gagaagagga acctcctcac ccccgggatg ctgctcgggc tcgagaaggc
      901 actcgggagg ctcgggaaag atggcaggac gaggtgtgtc gtgataaggg gggccgggga
      961 taaagcgttc tcctccggat acgacataag ctcgatcggc aaggacgaca tgataaaaaa
     1021 ccattcgggg gaaaacccgc ttcccagggc ggtaaaatcg atagagaatt tcccctaccc
     1081 ggtgatcgcc atgcttaacg gccacgcttt cggcgcgggg ctcgagatcg ccgccacgtg
     1141 cgacataagg acggcggcaa aaggatgcct cctcggtatg ccgcccgcga aactgggggt
     1201 catatattct tacaggggcg tacgtaaatt cctcaacctg atcggcccgg ggtacacgaa
     1261 ggaactcttc ctcacgggaa ggcccgtcga ttcggaaagg gccgagagaa tgggactcgt
     1321 caacgtcacg ctctccgcag aggtgctcga aaaatttact tacgatatgg ccggcgaaat
     1381 aacggagaac gctcccctct cccttaaggg gcttaaggag atggttaacg tttggcaaag
     1441 aaaccaggta atacacgggg aagacgaaga gttattaaag aaactgacgc ttagcgtaca
     1501 ggagagcgag gactataagg aggggcagag gtcgttcgcc gagaagagaa aacccatatt
     1561 taaaggagaa tgagtacggc cccgcctgcg gcccgcgcta attattttgt ttcttactca
     1621 gcctaaaaag gtcgagcctg ttgaagctca tttcggttat gtcatatttc ccgccgagcc
     1681 cggccctcgt attggccctg ctttccgggt gggcgtgaaa tacgaaccat atactcctcc
     1741 cgccgaggtt ctcgaaggtc gggaatttct ttccgagcgg gactatcctt aagtccttcc
     1801 ttttatggta gtactcggcc gtgactctct catagacggg cgatacgatg attatatccc
     1861 caagccccgc gtccgattcc acataggcca tcagctcccg ccattggtgc ttcccggcgg
     1921 tagtgaaata gtgcgcgata ttgtaagatg aaagaatcaa tatcagcccc gccgcagccg
     1981 ctatcggcca cctgttgttc atatttgcga tcccttttga cgcgagcaga tagaatgcga
     2041 gagaggcccc tatggtatag cggaatatca gtattggaga tgagacgaga gagataaggt
     2101 acggaacgag gacgggtaca gccatccata cgaggagtat gtagatcttt tctccttccg
     2161 ataaaccggc cgccggagcg gattcggtcg atatatttcc cgatttcttc acattcgcgg
     2221 acttgcctat cctgagaccg gcaaaggagt acagagaaaa caccaagaat acaatcaaaa
     2281 gatatgttga accggcatat atcctgaaaa attgccagat agcctccgag gagggctcag
     2341 ggacccagaa ccccttttgt atcttgagcg cgatcaccgc gagaaatacc atgcccggca
     2401 gcagaaccag acccgttaat gcttgcagct tgatccatcc gccgagcccg atctcaccga
     2461 tctttctttt cctcagcagc agtgtaaaac aaaaaatatt ctgggccagg attatcattg
     2521 tcccgtaata atgcgtgtaa acgaggagcg ccgtcgagac tacgtaaaca gcggcgtata
     2581 acgtactcct cctgattgtc agacggaaga aggagtagta agagacgagt ataagacaca
     2641 ccatgagggt atagccccta gcctcctgag cgtatttaat gtggaagaat gagactgcga
     2701 ggatgagcgc cgcaatgagc ccggttcgtt tgttgtagag gagcttgcct acagcgtata
     2761 tcgcgatcac ggagacggag ccgaatatag cggaaggaat cctggagaca gcttccgagt
     2821 ccccgaacat ggcggtccac aggctgagga tcatataata gaaaggcggg ttggtctcgg
     2881 ccgtattatc gaatacccac ttgattatgt ctttgaaccc gagcttggag acggatatcg
     2941 agaccgcttc gtcgtaccag atgccctcac ggccgagtcc gtaaaacctc aggacagctc
     3001 ccagaataac gattaagatt ataaggtaat tttctttcag gaatccggag aacaattctt
     3061 tcttcatgta tcagatgccg tagccgtaat ccttgatgag gttctccctc tccgtccatc
     3121 tctcgctgac cttcacccag agctcgagga aaaccttcgc tcccagtacc ttttcgatat
     3181 cctctcgcgc gaggctccct atcatcttca gcatctcgcc cttctttcct atgataatcc
     3241 ccttgtggtt catcctctcg acgtatataa cggccgagat cctgacgagc cccttctcgg
     3301 ggacgtcctt gaactcctcg atcacgaccg cggatttgta gggtatctcc tccctcgtca
     3361 actggaaaat cttctccctg atgaactcgg ccgcaaggaa cctctcgggc tggtcagtga
     3421 tcgtgtcttc cgggaaatat ttcggcccct cgggcaggta tccgactacc gtgtcgagga
     3481 gctcgcctat cccgtccgcg ttccttgccg atagaggaat gacttcgagg aatctgcctt
     3541 caaacctcct cgattcgtcg ataatccgga gtaaatcgcc ctttttcacg gtatctatct
     3601 tattgattat caagatcgcc ggcttgggaa gattctcgat tatgaaacgg tcccctttgc
     3661 cgaacgggtc cctgacctcg atcatcatga gtatcacgtc cgcttcctcg
//
LOCUS       DASSJH010000319         9676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_293672, whole genome shotgun sequence.
ACCESSION   DASSJH010000319 DASSJH010000000
VERSION     DASSJH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293672"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..364)
                     /locus_tag="VFJ67_06915"
     tRNA            complement(287..364)
                     /locus_tag="VFJ67_06915"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Glu,seq:ctc)
     gene            658..1248
                     /locus_tag="VFJ67_06920"
     CDS             658..1248
                     /locus_tag="VFJ67_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET7290157.1"
                     /translation="MQGITGDKEYSSIKKILTVKALLIVLLLIFAACDGGSDGGDTSP
                     GPTNSPPTNPPGGFEDTMLELINDARAEGRDCGSEFFPAAPPLSWDDRVAAAAEGHSE
                     DMAENRFFDHEGSDGSDTGDRLMNEGYDPSAWGETILVGIENEAAVVEAFFDSPDHCM
                     ILMDQSYEDVGVGAAEGIFKGQSTLYWTIDVATEKN"
     gene            1312..2925
                     /locus_tag="VFJ67_06925"
     CDS             1312..2925
                     /locus_tag="VFJ67_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7290158.1"
                     /translation="MTQDAKELGLITSGSLVEGLQMKLGPERSVEEVKAGKFVVVHGI
                     YNQFFSLITDVRLDASSPNILVNPPSMEEELLRSVLTGTSAYATIELRPMLMLGHEDR
                     ELRPVKTIPPHFASVSEASSADVSTVFGKEDGKGRFFNIGSPLDMDIPVCINLDRFVE
                     RSNGIFGKSGTGKTFLTRVMLAGVIMYGKAVNLIFDMHNEYGWETESEDSKGFVKGLK
                     QLFPEKVAIFSLDPESTRKRGRKPDVEAFISLGDIDIEDIALLQDELQLNTTAIESAF
                     AIYRRYGQKWLSELLSWEEEELADISKELGTHMQSLSAMRRKLNRIRALPFIVEDSIQ
                     SEGTIKKILDHLGRGISIVLEFGRSNSTLAYLLAANIISRRIHDYYIDRTERYHATKD
                     SSDKPRQLMITIEEAHKFLNSQAARQTIFGTIARELRKYSVTMLIVDQRPSGIDDEVI
                     SQVGTRITALLNDEKDIQSVLTGVSGTAGLRSVLASLDTKQQALILGHAVPMPVVVKT
                     REYGESFYKEISKVPEEGKGEELIGELYG"
     gene            complement(2957..3481)
                     /locus_tag="VFJ67_06930"
     CDS             complement(2957..3481)
                     /locus_tag="VFJ67_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET7290159.1"
                     /translation="MIFSLGERSVEIKGDDYYIAENAVVIGSVIIHNNVSIWYNTVIR
                     SDYEPITIMDDTNIQDGVVIHTDAGIPTTIGRGVTVGHQAMLHGCTIGDNSLIGINAV
                     LLNNSRIGRNCIIGANCLITQGKVIPDNSMVLGSPGKVVREVTPAEIEDLKSSAEHYV
                     NNFKRFKKELKRQD"
     gene            complement(3580..4461)
                     /gene="ispE"
                     /locus_tag="VFJ67_06935"
     CDS             complement(3580..4461)
                     /gene="ispE"
                     /locus_tag="VFJ67_06935"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET7290160.1"
                     /translation="MGSITLLSPAKVNLTLEVLGRRPDGYHEIRSLMQPVDLFDEVTV
                     TTTDGEGIELSSSGIEIPEGKDNLAWKAADVYLRECGLDTGVSISIKKWIPPGSGLGG
                     GSSNAAAVLTGLNRLTDALPEEKLFGLSPLLGADVAFFLRSVASVAEGIGERITVIKD
                     FPLFFYVILCPNLHVSTVDVYTKWNEMNAGNPPKKVLSEVDELIERFKGASGDFPLRN
                     DLEEAAFGLYPQIKSFKQILSSLDVKNVLMSGSGSAVYAVFRDELPAHEIYEYLKTSP
                     TFQVYSATGIRGWHRII"
     gene            5021..5671
                     /locus_tag="VFJ67_06940"
     CDS             5021..5671
                     /locus_tag="VFJ67_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290161.1"
                     /translation="MRLYEMQNQFGNFDANFELIDKGGLCLIFLNPVLESGDMVWLMK
                     NEPLRKEENGEGEMWTYLFEKQYLTGDGEGHEFDIPILVLMQSGMVKEIIFPERFLKN
                     ISIPLLKKMFTSMGEAEVNKLSKSASSVFKGGGPGDIPGMENIVDTLGTPYSIEEKDE
                     TTKYTYLYYLKNGQSVPVRDYFEFETEFVFDKESKSLEKATGTIRGLSMSLDFTAN"
     gene            complement(5717..6211)
                     /locus_tag="VFJ67_06945"
     CDS             complement(5717..6211)
                     /locus_tag="VFJ67_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET7290162.1"
                     /translation="MRHNRIEPAPGQESVWDYPRPPRVERSGKHVEVFFNNVLIADSS
                     DTLRVLETSSPPVYYIPRPDIEMKYLIPSSRMTVCEWKGAASYYTVSAYDKRAESAAW
                     HYPDPAPGYEAIRDHIAFYPQMMDACYLDGELVKGQPGKFYGGWITRDIVGPFKGEPG
                     TEGW"
     gene            complement(6252..7214)
                     /gene="egtD"
                     /locus_tag="VFJ67_06950"
     CDS             complement(6252..7214)
                     /gene="egtD"
                     /locus_tag="VFJ67_06950"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET7290163.1"
                     /translation="MKKNILQVEKFEPERERLVNEVITGFESGQKQLPCKLFYDKTGS
                     ALFDEICGLEEYYPTRTEIGIMEENIADICSLLGKNCLLVELGSGSSIKIRLLLDNLI
                     RPSGYVPIDISEEHLMESVQTLAGAYPGLRIMPVHADYTQPLSLPRFDFPFSHTVFFY
                     PGSTIGNFTPESAGAFLKRIAKRSGRGSGLLIGVDLVKDLKTLEDAYNDAKGVTAEFN
                     LNILRRLNREIGADFDMAKWKHKAFFNTRESRIEMHLESVTNQRVSVDGASFRFRKDE
                     TILTEYSYKYTIKGFRDLVSDFYSVEKVWTDRENKFSVQYLKVR"
     gene            complement(7268..8608)
                     /gene="egtB"
                     /locus_tag="VFJ67_06955"
     CDS             complement(7268..8608)
                     /gene="egtB"
                     /locus_tag="VFJ67_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET7290164.1"
                     /translation="MEPRSGFRTNSRHKHEGGSLQSKHRSREFLRESFKTVRDFSHTL
                     AEPLEIEDYVIQSMPDVSPTKWHLAHTSWFFETFVLSKAVKGYKSPSPQYAYLFNSYY
                     VQAGERHLRPKRGLISRPTVEETYHYRNHVDEHMTRFMEDADAREWKEFSPVIEIGIH
                     HEQQHQELIVTDIKHVFSENPLHPVYRPDGAPNGKAARKSPAANWVDIDGGVHSVGHE
                     GTGFGYDNEYPRHKVYLDPFRLQSRLVTNAEYMEFIEEGGYETPELWLSQGWATAESR
                     NWSSPFYWERKNGEWVHFTLSGMKAVNPEEPVCHVSYFEADAYARWAGDRLPTEAEWE
                     VAAGTVPLEGNFAESLSFHPEAPVTVKNGHDLRQMYGDVWEWTQSSYSPYPGFKTLPG
                     ALGEYNGKFMCNQYVLRGGSCATSESHIRKTYRNFFPPDARWQFMGIRLAKDGV"
     gene            complement(8844..9467)
                     /gene="hisG"
                     /locus_tag="VFJ67_06960"
     CDS             complement(8844..9467)
                     /gene="hisG"
                     /locus_tag="VFJ67_06960"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET7290165.1"
                     /translation="MITLAIARGRLLGEEVALLKRAGYPVDNILKDSRKLIFEYPSLK
                     LKILIIRPTDIPPYVEYGAADCGIVGVDTLMEEKHDLYEPLDLAIGKCKLVVAAPKGF
                     EYASTGSLRIATKYPRIAHEHFSGKGVGADIVKLYGSVELAPIVGLSDVIVDLTATGE
                     TLKKNNLIEFETIAEISARLVVNRVSMKVKSEELKALIRKLKEVRGN"
     gene            complement(9464..>9676)
                     /locus_tag="VFJ67_06965"
     CDS             complement(9464..>9676)
                     /locus_tag="VFJ67_06965"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940516.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7290166.1"
                     /translation="GNSAVVRGVKNLSGAPTMASDLRASASLVLAGLAAIGKTEISRV
                     YHLDRGYDKLDSKLETLGARIWRENE"
BASE COUNT         2189 a         2479 c         2619 g         2389 t
ORIGIN      
        1 tttgtgctga agcatatcca gatataaaga gaatcactaa taatgtaagg atgctaaaag
       61 ctttcatggc tcctcctctc atgactggtt tgatacgtaa gtataaaaat taagtgaaat
      121 tatagcattc tagtgcattc aacacaattt gttgttttat aagttcacta aaatatgaga
      181 cgtacattag ggtattagta tttattgtgc ccaaaaagcc tgcaatatgt attgtagagg
      241 ggctctgcgt atataaacaa tgttgatgta actaacagtg attatttggt agcccctacg
      301 ggattcgaac ccgtgtttta gccttgagag ggctacgtcc taggccaggc tagacgaagg
      361 ggccgtactt taaagcagga ttgttaattt atcataagga tacataaaag caacgagagg
      421 gggtatcggg cctcggctga atagcgggag acaatgggca taacgacggt ataatgtagt
      481 ttatctataa tgacccccat cctcacctat tttggtttag gcggcgttgc gttagaagca
      541 aagtcggata ggaggcattg ttggtgaggt gacctactgc tcacttgttc gcagcagttc
      601 caccgcctcg ggggaaggaa gtgaaagaaa ggcaaaacgg tcttacacat cgtgcagttg
      661 caaggtataa ccggagacaa ggagtatagc tcgataaaaa aaatactgac agtaaaagcg
      721 ctgcttatag ttttacttct gattttcgcg gcatgcgacg gcgggtcgga cgggggcgat
      781 acatcgcccg gccctacaaa ttcgcctccg accaaccctc ccgggggctt cgaggacact
      841 atgctcgaac tcataaacga tgcgagggcc gagggcaggg actgcgggag tgaattcttc
      901 cccgccgctc ccccgctcag ctgggacgac agggtcgcgg ccgcggcaga gggacattcg
      961 gaggacatgg cggagaaccg gtttttcgac cacgaagggt cggacggctc cgacacgggc
     1021 gacaggctca tgaacgaagg gtacgacccg agcgcctggg gcgagaccat actcgtcggc
     1081 atcgagaacg aagcggccgt cgtggaggcc ttttttgact ccccagacca ctgcatgata
     1141 ttgatggacc agtcgtacga ggacgtcgga gtgggcgccg ctgagggcat attcaaaggt
     1201 caatcgaccc tttactggac aatcgacgtc gcgaccgaga agaactgacc cgccgcccgg
     1261 gcttcttcct tgccaatgcc ctgacccttg gctattctga atgctgaata tatgacacag
     1321 gacgcgaagg aactcggttt gatcacgagc ggctctctcg tggaagggct acagatgaag
     1381 ctcggcccgg agaggagcgt ggaggaggtc aaggccggga agttcgtcgt cgtccacggt
     1441 atctataatc agttcttttc ccttatcacc gacgtgaggt tagacgcctc ttccccgaac
     1501 atactcgtta accccccgtc gatggaggag gagctcctga ggagcgtgct cacgggcacg
     1561 agcgcctacg cgactatcga gctcaggccg atgctgatgc tcgggcacga ggacagggaa
     1621 ctccgtccgg tgaagaccat ccctccgcat ttcgcatccg tatcggaagc gtcgagcgcc
     1681 gacgtgagca cagtgttcgg caaggaggac gggaagggca ggttcttcaa cataggcagt
     1741 ccgctcgaca tggacatacc ggtctgcata aacctcgaca ggttcgtcga gaggagtaac
     1801 ggcatattcg ggaagagcgg gaccgggaaa accttcctaa cgcgcgtcat gctcgcgggg
     1861 gttataatgt acggcaaggc ggtaaacctg atattcgaca tgcacaacga gtacggctgg
     1921 gagactgaga gcgaggactc gaagggattc gtaaagggtc tcaagcagct gttcccggag
     1981 aaggtcgcca tattctccct cgaccctgag tcaacgagaa agagggggag gaagcccgac
     2041 gtggaggcgt tcatatcgct aggcgacata gacatcgagg acatcgcgct acttcaggac
     2101 gagcttcagc tgaacacgac ggcgatagag tccgcgttcg ccatatacag gcggtacggg
     2161 cagaaatggc tgtccgagct actgtcctgg gaagaggaag agctggccga tatatcgaaa
     2221 gaactcggca cgcacatgca gtccctttcc gccatgagga ggaagctcaa cagaataagg
     2281 gcgctcccgt ttatagtcga ggacagcatc cagagcgaag ggacgatcaa gaaaatactc
     2341 gaccatctgg ggcgggggat aagcatagtg ctcgaattcg ggaggagcaa cagcacactt
     2401 gcgtatctcc ttgcggcaaa tatcatatcg agaaggatcc acgattacta catagacagg
     2461 acggagagat accacgctac caaggattcg tccgacaagc cgaggcagct catgatcacg
     2521 atagaggagg cgcacaagtt cctgaattcc caggcggcca ggcagacgat attcggcacg
     2581 atagcgcggg agctgaggaa atactcagtg acgatgctta tagtagatca gaggccctcg
     2641 gggatagacg acgaggttat ctcgcaggtg ggcacgagga tcacggcgct cctcaacgac
     2701 gaaaaggaca tacagtccgt gcttacggga gtgagcggga ccgcggggct gaggtccgtt
     2761 ctcgcgagcc tcgatacgaa gcagcaggct ctcattctgg ggcatgccgt tccgatgccg
     2821 gtcgtcgtga agacgaggga gtacggggag agcttctaca aggaaatctc gaaggttccc
     2881 gaggagggga agggggagga gcttatcggg gagctctacg gttgatcttg attcctgctg
     2941 taaggcaaac cactaactaa tcttgccttt tcagctcttt tttaaaccgt ttgaaattgt
     3001 tgacgtagtg ttcggccgac gacttcagat cctctatctc tgcgggcgtc acttccctta
     3061 cgaccttccc cggcgagccg agaaccatcg aattatccgg aattaccttg ccctgggtta
     3121 tgagacaatt cgcgccgatg atgcagttcc tgccgatgcg cgagttgttt agcaagactg
     3181 cgtttattcc tatgaggctg ttgtcgccga tagtgcatcc gtggagcatg gcctgatggc
     3241 cgacggtgac gcctctgcct atggtggtcg ggatgcctgc gtcagtgtgt atgacgacgc
     3301 cgtcctgtat gttggtatca tccattatcg ttatgggctc gtaatcgctc ctgatgacgg
     3361 tgttatacca gatgcttacg ttgttgtgga ttatcacgga gcctataact accgcgtttt
     3421 cggcaatgta gtaatcgtct ccttttattt cgacactccg ctctccgaga gaaaatatca
     3481 agtcggtcct cctttgccgc ctccgtcgat gtacgatcca ggtgaaacta ttttacataa
     3541 gcgagaatat tccgacctca tgactctttt cgtcattttt caaataatcc tgtgccagcc
     3601 ccttatgcct gtggcggaat agacctgaaa cgttgggctc gtcttgaggt attcataaat
     3661 ctcgtgcgcc gggagctcgt ccctgaatac tgcgtatacg gcggagccgc tccccgacat
     3721 gaggacgttc ttcacatcga gagaggatag aatctgctta aacgatttta tctgaggata
     3781 aagcccgaac gcggcctcct cgaggtcgtt gcggagaggg aaatccccgc ttgccccttt
     3841 gaaacgctcg attaattcat cgacttcgga caaaactttc tttgggggat tcccggcgtt
     3901 catttcgttc cacttggtgt aaacgtcgac cgtggatacg tggaggttgg gacagagtat
     3961 gacgtagaaa aagagcggaa agtcctttat cactgtgatc cgctccccga tgccttcagc
     4021 cacggaagcg accgagcgga ggaagaacgc cacgtccgct cccagaagcg gcgaaagacc
     4081 gaagagcttt tcttcgggaa gcgcgtccgt gagcctgtta agccctgtga gaactgcggc
     4141 cgcattgctg ctcccgccgc cgagacccga acccggggga atccatttct ttatggatat
     4201 ggagacgccc gtatcgagac cgcactcccg gagatacacg tcggctgcct tccacgcgag
     4261 gttatcctta ccctccggta tctctatacc gctcgaactg agctctatcc cttcgccatc
     4321 ggtcgtcgtt acggtcacct cgtcgaaaag atcgaccggc tgcatgagcg accttatctc
     4381 gtggtatccg tccggcctcc tgccgaggac ctcgagagtg agattgacct tcgcgggtga
     4441 gaggagtgtt atggagccca taggatctga ccgtggtaga atgtaatgga tatgtatgat
     4501 ttttcaacaa accatccaca agacggatac attgtaaata attctgcaat attcatattt
     4561 tttcaacgcg gattttttga ccgattttcg ttcaggacgc ggaaaattaa ggcctcctta
     4621 ggaattcaat aatacgcgtg attacggcat gttatgcgtg taataatcca tataatgtaa
     4681 atatgtcatg cgcgtaaaaa taattccttg acatttaaag tatcgtactt tacttttata
     4741 tataaattta aacgcagtac ttaaagtcat cctccatcct cctttaagag aacgggagta
     4801 attcttgact gagaattact cccgtttttg tatttaaata attctccgga actgtccctt
     4861 gttgttatcc aaaggcgtta tgagtaaagt atttgagagg cattccaggt catattttgc
     4921 ccgatcgctt acagcttaaa atcttctcat gaatccgaaa cggttaataa tacgctccat
     4981 attgctggct tcgattttac tcgttactgg ctgcgtgtat atgaggctct acgagatgca
     5041 gaaccagttc ggcaatttcg acgcgaattt cgagctcatc gacaaagggg gcctctgcct
     5101 catattcctt aaccccgtgc tcgagtcggg ggacatggtc tggctcatga agaacgagcc
     5161 gctcaggaag gaagagaacg gggaagggga gatgtggacg tacctgttcg aaaagcagta
     5221 ccttaccggg gacggcgagg ggcacgagtt cgatataccg atcctggttt tgatgcagag
     5281 cgggatggtc aaggaaataa tattcccgga gcggttcctt aaaaatatat cgatccctct
     5341 actgaaaaag atgttcacgt ccatgggaga ggccgaggta aacaagctct cgaaaagcgc
     5401 gagctctgta ttcaagggcg gcggtcccgg ggatataccg ggcatggaaa atatcgtcga
     5461 tactttaggc acgccgtatt cgatcgagga gaaggacgag acgacgaaat atacctacct
     5521 ctattacctg aagaacggac agtcagttcc cgtccgggat tacttcgagt tcgaaacgga
     5581 gttcgttttc gataaagaga gcaaatcgct cgaaaaggcc acagggacta taaggggcct
     5641 gagcatgtcg ctcgatttca cggcgaatta gatcggaaga gtcgatgagc gtgcagggct
     5701 taatcgatcg gtaacgctac catccctccg ttcccggctc tcccttgaac gggcctacga
     5761 tgtcccgcgt gatccagccg ccgtaaaatt ttcctggctg ccccttcacg agctctccgt
     5821 cgagatagca tgcgtccatc atctgcggat aaaaggctat gtggtccctt atggcttcgt
     5881 agccgggcgc ggggtcggga taatgccagg ccgcgctctc ggcgcgttta tcgtatgccg
     5941 agacggtgta ataggacgcg gcgcctttcc attcgcacac cgtcatcctg gagctcggta
     6001 taagatattt catttcgatg tccgggcggg gtatgtaata gacgggagga ctgctcgtct
     6061 cgagcactcg cagagtgtcc gacgaatcgg cgatcaggac attattgaag aagacctcta
     6121 cgtgtttacc cgatctctcg actcgcggcg gtctcgggta gtcccagacg gattcctgac
     6181 cgggtgcggg ttcgatgcgg ttgtgcctca tgactttggt gatttatccc gtgtgagtga
     6241 aatcgatgta actatcttac cttcaggtac tgtacgctga acttgttttc cctgtccgtc
     6301 cagactttct cgaccgagta aaaatccgac acgagatccc tgaacccttt tattgtgtac
     6361 ttgtaagagt attctgtgag tatcgtttcg tccttcctga aacggaagct cgcgccgtcc
     6421 acgctgaccc tctggttggt aacgctctcg agatgcattt ctattctgct ttcccgcgtg
     6481 ttgaaaaagg ccttgtgttt ccatttcgcc atgtcgaaat cggctcctat ctcgcggttt
     6541 agccgtctca ggatattcag attgaattcc gcggtcacgc cctttgcgtc gttgtacgcg
     6601 tcttcgagtg tcttcaggtc ctttacgagg tcgaccccga tcagaagacc gcttccgcgg
     6661 cctgaccgtt tcgctatcct cttcaggaac gcgcccgcgg attcgggcgt aaaattgccg
     6721 atcgtcgagc cgggatagaa aaagacagtg tgggagaagg ggaagtcgaa ccgcgggagg
     6781 ctcaggggct gagtgtaatc agcgtggacg ggcatgattc tgagccccgg ataagcaccc
     6841 gcgagcgtct gtacggattc cataaggtgc tcttcggaaa tatcgatcgg cacgtaaccc
     6901 gacggcctta tcagattatc gaggagcaat cttatcttga tgctgctccc gcttcctaac
     6961 tccacgagca ggcagttctt tccgaggaga gagcagatat ctgctatgtt ctcttccatt
     7021 attccgatct cggtgcgcgt cggataatat tcctcgagcc cgcatatctc gtcgaagagc
     7081 gcggagcccg tcttgtcgta gaacagcttg cacgggagct gcttttggcc gctctcgaac
     7141 ccggtaataa cctcgtttac gagtctctct ctctcgggct cgaatttttc aacctgcagt
     7201 atgtttttct tcatcggact ataagttata cgctccggag ggtcgtccgg atccacgcag
     7261 tcagagtcta tacgccatct ttcgcgagcc ttatgcccat gaactgccac ctcgcgtcgg
     7321 gaggaaagaa attcctgtac gtcttcctga tgtgcgactc cgaagtggcg caggagcctc
     7381 ctctcaggac gtactggttg cacatgaact tgccgttata ttcgccgagt gctccaggaa
     7441 gtgttttgaa tcccgggtag ggcgagtatg agctctgggt ccattcccat acatcaccgt
     7501 acatttgtct gagatcgtgc ccgtttttaa cggttaccgg cgcctcgggg tgaaaggaca
     7561 ggctctcggc gaaattccct tcgaggggca cagtccctgc ggctacctcc cactccgcct
     7621 cggtgggcag cctgtcgcct gcccaacgcg cgtacgcgtc ggcctcgaaa taggatacgt
     7681 ggcatacggg ctcttccgga tttacggcct tcatcccgga taaagtgaaa tgaacccact
     7741 ctccgttttt ccgttcccag taaaacgggg agctccagtt tctagattcg gccgtcgccc
     7801 agccttgcga gagccagagc tcgggagtct cgtatccgcc ttcctctata aattccatgt
     7861 attcggcgtt cgtgacgagc cttgactgaa gccggaaggg gtcgagataa actttatgcc
     7921 gggggtattc gttgtcgtat ccgaatccgg ttccctcgtg tcctacggag tgtacgcctc
     7981 catctatatc gacccaattg gctgccggcg attttctcgc ggccttaccg ttcggagcgc
     8041 cgtcgggcct gtaaaccgga tggagcggat tctccgaaaa tacgtgctta atatccgtta
     8101 ctatcaattc ctggtgctgc tgctcgtggt gtatgcctat ctcgatgacc ggggagaact
     8161 ctttccattc cctcgcatcc gcgtcttcca tgaacctcgt catgtgctcg tccacgtggt
     8221 tcctgtaatg ataggtttcc tccaccgtcg gtctcgatat gagacctcgc ttgggcctga
     8281 gatgcctctc tcccgcctga acgtaatagg agttgaagag gtatgcatac tgcgggctcg
     8341 gagatttata acccttcacg gcctttgaga gaacgaacgt ctcgaagaac cagctcgtat
     8401 gcgccaggtg ccatttcgtg gggcttacgt ccggcatgga ctgaatgacg tagtcttcta
     8461 tctccagagg ctctgccagc gtatgggaga aatccctcac agttttaaaa ctttctctca
     8521 ggaattctct cgagcggtgt ttcgattgga ggctgccgcc ttcgtgttta tggcggctgt
     8581 tagttcggaa tccggacctg ggttccataa tatttctccc ggtattaacc cgcctccgga
     8641 aaaagactga aggggatcgt ctaagtttaa ggtttaatga aagtatatac aaatatttag
     8701 tttaccgaat cttgtcctta ttcaaagcct ggaaaaatta agggtgtgtt aagggcgggc
     8761 gttaaataat tgtgttttgg cgatgatttt ttatcgggcg tagtttacgt aattaaccct
     8821 tgaatgttcg cggcggaggg cattcagttc cctcttacct ctttcaattt tcgtattagt
     8881 gccttaagct cttcggattt gaccttcatg ctgaccctgt tgacaacgag cctggccgag
     8941 atctccgcaa tcgtctcgaa ctcgatgagg ttattctttt tgagcgtctc cccggtcgcc
     9001 gtgaggtcta ctataacatc cgagaggccg actatgggtg cgagctcgac agagccgtaa
     9061 agcttgacta tgtccgcgcc tacgcctttc ccggagaagt gctcgtgggc tatcctcggg
     9121 tatttggtcg cgatcctgag cgagccggtc gatgcgtatt cgaagccttt cggggccgcg
     9181 accacgagct tgcatttgcc tatggcgagg tctagcggct cgtagaggtc gtgtttttcc
     9241 tccatcagcg tatccacgcc gactataccg cagtcggccg ccccgtattc gacatacggt
     9301 ggtatatccg tcgggcgtat aatcagaatc ttcaacttga gggacgggta ttcgaagatg
     9361 agcttcctcg agtcctttaa aatattgtct acgggataac ccgccctttt gaggagcgca
     9421 acttcctcgc cgagcagcct tcccctcgcg atcgcaagcg ttatcattcg ttctccctcc
     9481 agatcctggc tcctaatgtt tcgagtttgc tgtcgagctt gtcataccct ctgtcgagat
     9541 ggtaaaccct ggatatctcc gtttttccga ttgctgcgag tcccgcgagc acgagagacg
     9601 cgctcgccct gaggtcgctc gccattgtgg gcgcgccgct gagattcttc acgcctctta
     9661 cgacggcgct gttccc
//
LOCUS       DASSJH010000320         4509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1375779, whole genome shotgun sequence.
ACCESSION   DASSJH010000320 DASSJH010000000
VERSION     DASSJH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375779"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="VFJ67_06970"
     CDS             <1..1683
                     /locus_tag="VFJ67_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7290167.1"
                     /translation="AIVEKVYGSADLNLIFETFLDMIGGMKMIDIMCVYLVEGEGDER
                     EAVLRIQRGLPAEYIEKAGRIPYPKGITWEVINKGEPVYFRSLPGEPCPLGPAGKALS
                     PGVVLSLPLKRKGQTIGVVHFTRKKRGEFEKYQLDVLHSLGNQIGTAVSKAKMFEESG
                     QRAKELEWLYRDLKATQDQLIQSEKLASLGQLVSSIAHEINNPLTPILGYAQMLIASP
                     ETEEEKRQRFIEVIYSSADKVRKIVENLLSFARKDKPRREYVNINDILKNTVELRQYQ
                     LELDNIGVVMDLDADLPRTMADSTQIDQVFTNIIMNACHAISGTGARGGTITIKTRTG
                     TSGDIEAVITDTGPGIPEEIIHRIFDPFFTTKPTGVGTGLGLSVSYGLMKEHSGEILV
                     ESEPGKGASFIVRLPVRDYKDYLLVEEEDENGTGGEEAVPERKGRRVLVVDDEELVTM
                     LVEGILEGEGFDADFVTNGEEALSLIKKVEYSIIICDIKMPQMNGMEFYRRVKEMNAD
                     LAGRMLFMTGDTGPETMEFVNGTGNRLLAKPFKIEEFRDALKLVEDAGQRPL"
     gene            1747..2733
                     /locus_tag="VFJ67_06975"
     CDS             1747..2733
                     /locus_tag="VFJ67_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290168.1"
                     /translation="MRHILRGITALALLSLLLPAAFAGDYTVETYTSGVLSSEPGIKS
                     ALIGSGEELAEAKEALGITAPLPGVDFGKEALLLILSPEGSGGIIEVTGAVPAEGALE
                     VRYRVKSEGPAPMSGTKTSYPYLMAKINPAPADGVPVRFIDEDYANSLASGTGLGQFS
                     EYTNVLSGSEESEMTEFLPLDKGNSWTYITGEGGEESEVTNSVVSESDGWSVFDSFFG
                     LSGVGMKISPGGEVFVSSGGGIKTFYTPDVVTEFRKGPVNTPAGAFSNVMVVTIPEGG
                     GFWFMDVYAKGVGLITHEQNSRKGRVSYRLVRAVVGGKEYPRPDNGRATGNK"
     gene            3147..3500
                     /locus_tag="VFJ67_06980"
     CDS             3147..3500
                     /locus_tag="VFJ67_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HET7290169.1"
                     /translation="MTELAKFDPAQVIEIPKELYGDGFIVIRNRGDSMEKLIMDGASV
                     VIDVSSKEIVSGSIYVLSIPKEGCIIRECHSVSQGLLLRPYNRNYPASLVSWDEFDPE
                     MVLGRVFCSVINVFR"
     gene            3642..4088
                     /locus_tag="VFJ67_06985"
     CDS             3642..4088
                     /locus_tag="VFJ67_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290170.1"
                     /translation="MSKEEVKFSEGLDPLNEGEGFISYRERVMGLDAVVGFHFLRGSL
                     VEAGYAFREPLGGEHAYIREYRNLKGLLTGSYGRPSYDEGACGECGGFCVRCSGEECA
                     ETCSLVYLCEWTTPRSVIRLVLMGEGRGFDFGILHRSREHEKLVVK"
     gene            4328..>4509
                     /locus_tag="VFJ67_06990"
     CDS             4328..>4509
                     /locus_tag="VFJ67_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290171.1"
                     /translation="MTRASVQVLSFAFLLLCAGSIYAAAKPPDVSGWGNLKWGMTGDE
                     IKTAYGKDIKVHKPRE"
BASE COUNT         1036 a         1143 c         1431 g          899 t
ORIGIN      
        1 gcgatagtgg agaaagtgta cggctcggcc gacctgaacc tcatattcga gactttcctc
       61 gatatgatcg gcggcatgaa gatgatcgac atcatgtgcg tgtacctcgt ggaaggggag
      121 ggcgacgaga gagaggccgt gctgcggatc cagcgcggac tccccgccga atacattgag
      181 aaggccggga ggattcctta tcccaaaggg atcacctggg aggttataaa caagggcgag
      241 cccgtttatt tcagaagtct cccgggcgag ccgtgtccgc tcgggccggc ggggaaggcg
      301 ctttcgccgg gggtcgtcct ctcgctgcct cttaagcgca aggggcagac gataggcgtc
      361 gtgcatttca cgagaaagaa acggggggaa ttcgagaaat accagctcga cgtcctccat
      421 tccctcggca accagatagg gacagccgtc tccaaggcca agatgttcga agagtccggg
      481 cagcgcgcga aggagctcga atggctgtac cgcgacctga aagcgacgca ggaccagctc
      541 attcagtccg agaagctcgc ttcactgggg cagctcgtat cgagcatcgc gcacgagata
      601 aacaacccgc tcactcccat acttggctac gcgcagatgc tcatcgcctc gcccgaaacg
      661 gaggaggaga agaggcagag gttcatagag gtgatataca gctccgctga caaggtaagg
      721 aagatagtcg agaaccttct gtcgttcgcg aggaaggaca agccgcggag ggaatacgta
      781 aatatcaacg atatactgaa gaatacggta gagctcaggc aataccagct cgagctcgac
      841 aacatcggcg tcgtcatgga cctcgacgcg gatctcccca ggaccatggc ggattccact
      901 cagatagacc aggtgttcac gaacatcata atgaatgcct gccacgccat cagcggtacg
      961 ggcgcccgcg gcgggactat cacaataaaa acgaggacgg ggacgagcgg cgatatcgaa
     1021 gcggtgatca cggacaccgg gcccggcatc cccgaggaaa tcattcacag gatattcgac
     1081 ccgttcttca cgacgaagcc tacgggcgtc ggcacggggc tcggtctctc ggtctcgtac
     1141 ggattgatga aggagcacag cggggagata ctcgtcgaga gcgagccagg gaaaggggcc
     1201 tctttcatcg taaggctgcc ggtcagggac tacaaggact atcttctcgt cgaagaggag
     1261 gatgagaacg ggacgggagg ggaagaagcc gtgcccgaga ggaaaggacg gagggtgctc
     1321 gtggtcgatg acgaggagct cgtaacgatg ctcgtggaag ggatactcga aggagagggc
     1381 tttgacgccg atttcgttac gaacggcgag gaagcgctgt cgctgataaa gaaggttgaa
     1441 tacagcatca tcatctgcga tataaagatg ccgcagatga acgggatgga gttctacagg
     1501 cgcgtgaagg aaatgaacgc ggaccttgcc gggcgtatgc tgttcatgac cggggacacg
     1561 ggcccggaga cgatggaatt cgtaaacggc acgggcaaca ggctgctcgc gaaacccttc
     1621 aagatagaag aattcaggga cgccctcaag ctggtcgaag acgccggcca gcgtccgctt
     1681 taacatcccg caagccctct tttggaagta taattgttat aaaccggtca atcgacggac
     1741 gtccctatga gacacatcct ccgcggcatc accgcgttag cgctgttatc gctcctcctc
     1801 ccggcggcct ttgcagggga ttacacggtc gagacatata cgagcggcgt gctcagcagc
     1861 gagccaggga taaagagcgc tctcatcggg agcggcgaag agctcgctga ggcgaaggag
     1921 gcgctcggca taacggcccc cctccccggg gtcgatttcg gaaaggaggc gctcctgctc
     1981 atactctcac ccgaaggcag cggcggtatc atcgaagtga cgggtgcggt ccctgccgag
     2041 ggcgcgctcg aggtcagata cagggtcaag tccgaaggcc ctgccccgat gtccggtact
     2101 aaaacctctt acccgtatct catggcgaag attaatcccg cgccggccga tggcgtgccc
     2161 gtcaggttta tcgacgagga ctacgccaac tccctcgcat cgggcaccgg gctcggacag
     2221 ttcagtgaat acacgaacgt cctttccgga agcgaagaaa gtgagatgac ggaattcctt
     2281 ccgctcgata aaggaaactc ctggacatat ataaccgggg agggcgggga ggagtccgag
     2341 gtcacgaatt cggtcgtttc ggaatcggac gggtggtcgg tcttcgattc gttcttcggc
     2401 ctctcgggcg tcggcatgaa aatatccccg ggcggcgaag tattcgtctc ctcaggcggc
     2461 gggataaaaa cgttctatac gcccgacgtc gtgaccgagt tccgtaaagg gcccgtaaac
     2521 acgcccgccg gagcgttcag caacgtgatg gtcgtgacga tacccgaagg tggcggcttc
     2581 tggttcatgg acgtttacgc gaagggcgtg ggcctcatca cgcacgagca gaattcacga
     2641 aagggccgcg tctcctatcg gctcgtccgg gcggtcgtcg gcgggaaaga gtatccccgg
     2701 ccggataacg gccgggccac gggtaataaa tgagcgtttc cccggggcga cgggcccgct
     2761 gtcctgacta tggatcggcg tctctccgta atttcatgat tcccgcagta tcctgaaaat
     2821 ttttttatcg gcgcattatc ccggcttgta gggatgttac gcggcctttc tacatagata
     2881 ccctatacaa aatacaggca tcacccgatt gaaatgcccc cgccgttttc ggataattga
     2941 ttgcaggcac aaattagacc gcgattatga gagggggact ctccaacacg ggcggctgaa
     3001 acgcctgact acggcttaag ggtttccttg ccgctggcga gcatggaatc caagccgttc
     3061 gatagtccgt ataaattcaa ggtaactgct ccccctcgcg cgggaagagc gatgtctgat
     3121 ttgaccgcgg gaagtgagag gttctattga cggagcttgc gaagttcgat cccgctcagg
     3181 tgatcgagat accgaaggag ctgtacgggg acgggttcat cgtgatccgg aacaggggcg
     3241 attcgatgga gaagcttatc atggacggcg cgagcgtggt tatagacgtt tcctcgaagg
     3301 agatagtctc gggctcgatc tacgtgctct ccataccgaa ggagggatgc attataaggg
     3361 agtgccattc ggtatcgcag gggctcctgc tcaggcccta caacaggaac tatccggcct
     3421 cgctcgtcag ctgggacgag ttcgatccgg agatggtcct gggcagggtg ttctgcagcg
     3481 tgataaacgt attcaggtag cgcggctgcg gttgcttcgc attcggtaac ggaggactca
     3541 tcggcgctga aagactcgaa ggagggagag atatgaatac cggattcgca tttcacggtt
     3601 atgcgcagga gtttcgcgac ttccgagggg tgtcctgggg aatgtcgaag gaggaggtca
     3661 aattctcgga aggcctcgat cccttgaatg agggagaggg gtttatatct tacagggagc
     3721 gggtaatggg actcgacgcc gtagtgggct ttcatttcct ccggggctcg ctcgtcgagg
     3781 cggggtacgc gttcagggag ccgctcggag gcgagcatgc gtatatacgg gagtaccgga
     3841 atctgaaagg actgctcacg ggctcttacg ggcggccttc atatgacgag ggagcgtgcg
     3901 gcgagtgcgg ggggttctgt gtccgctgct cgggggagga gtgtgctgaa acgtgttccc
     3961 tcgtctattt gtgcgagtgg acgacgccgc gctcggtcat aaggctcgtg ctcatggggg
     4021 aggggagggg gttcgacttc ggcatcctcc acaggtcgag ggagcacgag aagctcgttg
     4081 tgaagtaaaa acagatgcat aagagaatcc gggtaagtga ggactagggc tgaggatatg
     4141 agtattatcc ccctttttct aaggagggaa ttaaagaatt aaggctggat tatcgtgcgg
     4201 tatttatcct gatcgaggac attcgcagcg ttcttgcctt ataaaaacaa tttccgtttt
     4261 cacaccctgt attttatagt tctagtatgg gaacgttccc tgccattttg aataccggga
     4321 ggtcgttgtg acaagagcgt cggttcaggt cctatcgttc gcgtttttat tattgtgcgc
     4381 cgggagtatt tacgcggccg ccaaaccccc cgacgtctcc ggctggggga atctcaagtg
     4441 ggggatgacc ggggacgaga taaagacggc atatgggaag gacatcaaag tgcacaagcc
     4501 gagggaaga
//
LOCUS       DASSJH010000321         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_77898, whole genome shotgun sequence.
ACCESSION   DASSJH010000321 DASSJH010000000
VERSION     DASSJH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77898"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..633
                     /locus_tag="VFJ67_06995"
     CDS             259..633
                     /locus_tag="VFJ67_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290172.1"
                     /translation="MYDPAYAEETVAEAVAKEMPVLKGDQWLTMDENAKVAFIWGAGH
                     VVTIEEVIMEKYPDQKRDTFVAKVVEASTNSPMTMSQVVAMVDKFYQDNPDKTDTPVM
                     AVIWESMIKPNIKTGIAGKPLK"
     gene            666..884
                     /locus_tag="VFJ67_07000"
     CDS             666..884
                     /locus_tag="VFJ67_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HET7290173.1"
                     /translation="MKKIKYLILAASIILTVAGCTNMSKTQQGTLSGGAIGAGAGLGI
                     AALSGGNLWVGTAVGAGAGALTGYMISK"
     gene            999..1625
                     /locus_tag="VFJ67_07005"
     CDS             999..1625
                     /locus_tag="VFJ67_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET7290174.1"
                     /translation="MKKYAVEFIGTFFFVLTVGLTVIEPGAGVMAPLAIGSVLMVMVY
                     AGGHISGGHYNPAVTLAVWMRGRCSSSDAPIYMISQWAAAVAAGFLALYLKGMPEIAP
                     MTPNVVYALIVEFLFTFALAYVVLNSATSKNTAGNSFYGLAIGFTVVIAAYSAGAISG
                     GAFNPAIALGITVMGISAVSNIWIFLVANFLGGAAAALAFNSLNPDDK"
     gene            1853..2581
                     /locus_tag="VFJ67_07010"
     CDS             1853..2581
                     /locus_tag="VFJ67_07010"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HET7290175.1"
                     /translation="MEVKDAPRIDPESMSDLVESLEFLSKAGKFSFTSETEYDAFQGN
                     GQKIEFGSVHKITVERPDKVYAEVEERDGTKKVFIFDGKDIYFSDFERNVYASVHRPG
                     DINQAIDYFTEDLQMPLPLGQLISTDVAEMIKKEVYAGGFVEESTINGVPCEHLAFRT
                     QDIDFQVWVTSEGDPLQMRLVIDYKDSPGSPQFRATYKDWNFKPAVSDSLFVFKPGDG
                     MQQIAFAPLLRQEIKTGVKEGEKK"
BASE COUNT          654 a          599 c          800 g          627 t
ORIGIN      
        1 ttttgtgaag ttgaaaaatg gctgtcggct cttgagcgta aggcccggag ccgcgaccgc
       61 agcctcaccc gcagctttcc gcgatctccg cttaacaaac ggggcatcgc agggcccgag
      121 tttaatcaga tagcgagcac agttaaaaat ttttgcagcc ggaataaatc attcctataa
      181 ggaggtagtc tcatgaagag ggcgattgcg attcacattt tcattttgtt actcgcttta
      241 agtgcagcag gctcccgaat gtatgacccg gcgtacgctg aggagactgt agcggaggcc
      301 gttgcgaagg agatgcctgt gctcaagggc gaccagtggc tgaccatgga tgagaatgcg
      361 aaggtcgcgt tcatctgggg tgccggtcac gtcgttacga tcgaagaggt gataatggaa
      421 aaatatcccg accagaagcg agatacgttc gtggcgaagg tcgtagaggc gtcaacgaat
      481 tctccaatga cgatgagcca ggtagtcgcg atggtcgata agttctacca ggataaccct
      541 gacaagacgg atacgccggt gatggctgtg atatgggaat cgatgataaa gcccaacata
      601 aagacgggaa tcgccgggaa gcctctgaaa taagctgacg ggactttaag taaaggagat
      661 cgaaaatgaa aaaaataaaa tatctgattc tagcagcgtc gataatcctt acagtcgcgg
      721 gatgcaccaa tatgtcgaag acccagcagg gcacactgag cggaggtgcc ataggagcgg
      781 gcgccgggct cggaattgcg gctctttcgg gcgggaacct ctgggtgggg acggccgtgg
      841 gggcgggcgc cggggctctc acgggataca tgataagcaa atgagaccag ggacggcttc
      901 acggccgtcc ctgattcctg taaccgggtg acagggattt atgctcgaag aatatgcgcg
      961 tgacggattt atagatctgc aagagggagg gtaatgatat gaaaaagtat gctgtcgagt
     1021 tcataggcac gttttttttc gtactgacag tgggtttaac tgtgatcgag cccggggcgg
     1081 gagtaatggc tccacttgct ataggctcgg tgcttatggt tatggtatat gcgggcgggc
     1141 acatctcggg cgggcattat aatccggccg tgactctcgc cgtgtggatg aggggaaggt
     1201 gctcttcgtc agatgcgccg atctacatga tttcccagtg ggctgccgcg gtagcggcgg
     1261 gctttctcgc cctctatctg aaaggtatgc cggagatcgc gccgatgacg cccaacgtag
     1321 tttacgcact catagtggag tttctcttca ctttcgcgct cgcttatgta gtgctcaact
     1381 cggcgacatc gaaaaatacg gcgggcaact cattctacgg cctggcgata ggctttacgg
     1441 tcgtgatcgc ggcctactcg gccggggcca tatccggcgg ggcgttcaat ccggcgatag
     1501 ctctcgggat aacagtgatg ggaatatccg cggtgagcaa tatctggatc ttcctcgtcg
     1561 cgaatttcct gggcggagcc gctgcggcac tggcattcaa ctcgctcaac ccggacgata
     1621 aatagcggcc cgtgattgat acagccgaac gtttgaacga attacgttta tcggtccaga
     1681 cccaccgttc aggcgtatat atggggtatt gctaaatggt ttgctaattc ataagtcata
     1741 gaatgaattc agggggtgtg ttatgaagag acgagtgtta caaatatttt tctgttcctt
     1801 tatttttttc attttgcctg ttttaccggt tgttcctcag actgagcagg gcgtggaggt
     1861 gaaagacgcg ccgaggatag atcccgaatc catgtcggat cttgtggaat cgctcgaatt
     1921 cctgtccaag gccggaaaat tcagctttac ctcggagacg gaatacgacg catttcaagg
     1981 aaatgggcag aagatagagt tcggcagcgt ccataagatt acggtcgagc gtcccgacaa
     2041 agtatatgcg gaagtagaag agagggacgg gacgaaaaag gtgttcattt tcgatggtaa
     2101 ggatatttac ttctctgact tcgagcggaa cgtttacgcg agcgtgcata ggccggggga
     2161 catcaaccag gccatagact atttcaccga agatctgcag atgccgctcc cgcttggaca
     2221 gctcatatcg accgacgttg ccgagatgat caaaaaggag gtatatgcgg gagggttcgt
     2281 cgaggagtct acaataaacg gcgttccctg cgaacacctc gcgttcagga cgcaggacat
     2341 cgattttcag gtgtgggtaa cgtctgaagg tgacccgctt cagatgaggc tggtcataga
     2401 ttataaggac tctccggggt cgccccagtt cagggctacg tacaaggact ggaacttcaa
     2461 gcctgcggta tctgattctc tgtttgtgtt caagcccgga gacggaatgc aacagatagc
     2521 tttcgcccca cttctccgtc aggagataaa gaccggggtg aaggagggag agaagaaatg
     2581 agaatcgaca ggaaattgac gatgaggctg tttattttca tggtgatctt tttatttttg
     2641 ggaagcatgg ttttggacgc ctacgccaga ggaagaggca
//
LOCUS       DASSJH010000322         9047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_294446, whole genome shotgun sequence.
ACCESSION   DASSJH010000322 DASSJH010000000
VERSION     DASSJH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294446"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..985)
                     /gene="rimK"
                     /locus_tag="VFJ67_07015"
     CDS             complement(77..985)
                     /gene="rimK"
                     /locus_tag="VFJ67_07015"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HET7290176.1"
                     /translation="MKIVVLSRNNKLYSTRRLVEAAGMMGHEVEVIDFLKCYMAIKRG
                     NPEVYHYGKKLKGVHAVIPRIGASRTFYGTAVVRQFEMMHVFSVNESQAIARSRDKLR
                     SMQILARAGIGMPVTAFASDPTDIGHLIELVGGAPVVIKLLHGTQGIGVVLAETKKAA
                     RSVMEAFYGVKANMLIQEFIKEAKGSDVRAFVVGGEVIGAIKRQGEEGEFRSNLHRGG
                     TAKMIKLTRQELQTAVNAARLLGLNVAGVDMLQSSKGPMVLEVNSSPGLEGIEAATGI
                     DVASKIIKFIEDNAHVRSMRRDRIRA"
     gene            1954..3033
                     /locus_tag="VFJ67_07020"
     CDS             1954..3033
                     /locus_tag="VFJ67_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HET7290177.1"
                     /translation="MNTSAIQPRIAGISTVVPSYSFSQKFAIDAARKWLSSGGEPELS
                     VSRTVQVFENAGVEKRYSVTPVEDMLREKDFPERNALYSKFARLLGEKALRDLFEKTG
                     VKPYEIDLFITVSCTGFMIPALDAYLMNRFPFRNNVRRLPVTELGCAAGVSSLILASD
                     YIRAYPEANVLILSLELCTLNMQPTDKSADHIISTAIFGDGAAAAIITGREDAAGLHI
                     LKTENRFFPGTLEFMGYDVEKTGFHIFLSPRISKFIESNIASEMEQMLGQAGLKIGDV
                     DDWLFHPGGSRILKSIEKGLRIDPAKLGESTAVLRDYGNLSSATIYFILDSFMRSESK
                     KNGSYQVVGAVGPGFGLDAILTQWK"
     gene            3053..4456
                     /locus_tag="VFJ67_07025"
     CDS             3053..4456
                     /locus_tag="VFJ67_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7290178.1"
                     /translation="MSIHGTSIAPEGQGFDAAPSSEDTDALYSKHVNPQWVKLYNTLG
                     MNVRYESCLGSELTTGDGRRILDFLSGYCVYNAGHNHPGIIEALTDELGSGGATMLQS
                     HVPGLAAELASRLCSLAGGRLNKIFFTSSGSEGVETVIKFSRAHTKRPGVLYCEGAFH
                     GLTCGALSLMDGPYWKEGFGPLLPDTESVPFLDLGALEEKLRSGRFAAFIVEPVQAEA
                     GILVPPSGRLKEAEALCRKYGTLFVLDEVQTGIYRTGPFLAAHHWGLEPDMVVLAKAL
                     SGGFVPCGAVLMSDGISRSVFSTVTRAFVHASTFGENNLAMRAGLATLDVMKEERLGA
                     RALSLGERLRKRLGEVSRGYEMVRGINGLGMLNGIEFAEPKRLRLRIPYETFRKIHPG
                     MFGMIVVMRLFREKNILTQICGNNHMVLKVAPPLVVTDAQIEEFVAGVRDVLETVHTK
                     TSFWSEALLMARRALGS"
     gene            4517..5221
                     /locus_tag="VFJ67_07030"
     CDS             4517..5221
                     /locus_tag="VFJ67_07030"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7290179.1"
                     /translation="MRVKSYAAFILSAAFIVSFIPSAGLASQDTAPGSGGGVTPDVYA
                     GGRVTEFEVVAGKSSVEFTGTSLMHHFHGVSHDPSGFTSVDFGNPRATTSAEITVPVD
                     SIKGIALGSEKSDLSKNIHENLESEKYPEIKFKVTQILPESTDASAPSKRSYLLKGDL
                     TIHNMTKPVVLTADTEIKDGYLHVTGEYDNLDMTDYGVEPKPLFAVVSVNDVVDVKFD
                     LYEDLRESAPVHSSVR"
     gene            5268..6359
                     /locus_tag="VFJ67_07035"
     CDS             5268..6359
                     /locus_tag="VFJ67_07035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7290180.1"
                     /translation="MESDVIVIGAGPGGSASAISFLRRGFGVTVLEKRFMPRTKVCGE
                     MVAPLGVKELGRLGVLAGIEAAGANRVRKVVFRSSGGAGTEIPFPGGEYGVAMSREAF
                     DSMLFAEARERGALGIEGFRVENVERERGGKFRVEGTRTETGERETYRSRAVINASGL
                     MGIGYRDKKCREFLYAFKIHLRGITCGDAAEIFFYDGGYGGLVDIEGGIVNLAFQIRK
                     DQMPLMKGHPLDILTAVLHSGHPVREKLSNAVPVGEWEAMGSVFVRKRGSFPGVWNVG
                     DSSGIIDPFTGLGISLALQGGRYIGENLEVGRVRDTYDLKRSLNRTRLMTAALLRKAL
                     FRPRLCHMIMKHASISPMLGMIVLGLLHT"
     gene            6854..7918
                     /gene="corA"
                     /locus_tag="VFJ67_07040"
     CDS             6854..7918
                     /gene="corA"
                     /locus_tag="VFJ67_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET7290181.1"
                     /translation="MTKPMKTAAMKKGLHPGELIHVGERKVEHVRMTVMDYDRDMFIE
                     REYKLVEECLEYADRDTVTWINVNGLHDTQIISSIGEHFGIHPLVIEDILDTEQRPEL
                     DDYLDYISLMLKMILVEKDTGRIDAEQISILLCRNTVISFQEGDGDVFEPIRERIRTG
                     KGRIRESGADYLMYILTDAVVDNYFVALEEIGELIGAIEDEVLEKPNAETLMEIYELK
                     RELVLLRKYTFPLRELVHKLQISESPLIDPSTKLYVRDLYDHAMQIMDTTESYRDMIT
                     GMLETYLSLSSNRMNEVMKVLTVMATIFIPLTFIAGIYGMNFENMPELHHHLGYPGVM
                     LLMLIVGLGMVYYFRRRKWI"
     gene            8495..9031
                     /locus_tag="VFJ67_07045"
     CDS             8495..9031
                     /locus_tag="VFJ67_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7290182.1"
                     /translation="MYKDNETVSVMVEITKGSRNKYEYDKVKKRIKFDRLLYASVHYP
                     SDYGFIEDTLGGDGDPLDALVLLWEPTFPGCIILSKPIGMFMMGDEKGPDEKILCVPV
                     DDPHWNHIEKLTEIPPHLLREIEHFFASYKTLEKKNVHVEGWRNKEAALQTIQEARDR
                     YKHKGKKKKKAKKAVDWL"
BASE COUNT         2143 a         2275 c         2719 g         1910 t
ORIGIN      
        1 agtcgatctc gatgcggagg aattccttcc cgcctttcgg gatgagcctc ccgtttatct
       61 ttatcgccct cttcaactat gcccttatgc ggtcccgcct catgctgcgg acgtgcgcgt
      121 tgtcttctat gaatttaata attttggagg cgacgtcaat tccggtcgcg gcttcgatgc
      181 cttcgagacc gggtgaggag ttcacctcca gcaccatcgg gcccttcgac gattgcagca
      241 tgtccacgcc ggcgacgttc aggccgagga gcctcgccgc gttcaccgcc gtctggagct
      301 cctgccgcgt gagctttatc atcttcgcgg tgccgcccct atggaggttc gagcggaact
      361 cgccttcctc tccctggcgt tttatcgcgc ctatgacctc gccgccaacg acgaacgccc
      421 tcacgtccga gcccttcgcc tccttgatga attcctggat gagcatgttc gccttgaccc
      481 cgtagaatgc ctccatgacg gagcgcgctg cctttttcgt ctccgcgagc acgacgccaa
      541 tgccctgcgt gccgtggagg agctttatga cgactggcgc gccgccgacg agctcgataa
      601 ggtggccgat gtccgtgggg tcggacgcga aagcagtgac gggcatgcca atccctgccc
      661 tggcgagtat ctgcatgctg cggagtttgt cgcgcgagcg cgctatcgcc tgcgattcgt
      721 tcacggagaa gacgtgcatc atctcgaact ggcggacgac ggcagtgccg tagaacgtgc
      781 gggacgcgcc gatgcgcggt atcaccgcat gcacaccctt gagcttcttg ccgtagtgat
      841 agacctcggg gttgcccctc tttatcgcca tgtagcattt gaggaagtcg atcacctcga
      901 cttcgtgtcc catcatcccg gcggcctcga cgagacggcg cgtggagtag agcttattgt
      961 ttctcgaaag tactactatt ttcataaaac tctcctgcgt gttgcaatac cgattacgaa
     1021 atctcgaatt ccggtcggtc aggcgaagcg cctgacgata tctgcggttg attgcctgtc
     1081 ggaagcttct ctctcgcaca tagtatgaca taaaaaaaga ggagaggaag tgggaattat
     1141 ttttgggaaa tatgatgtcc ggctacgcct ttggtttcgc cgtgacaagc aggatgcggg
     1201 atacaggatg caggacgaat gagttaaaga aaaaggaaaa tataaatcct ccctggccct
     1261 cctttttcta aggagggaat taaaggcaaa ggatgagatt gccgcgccgc gctgactctc
     1321 gtcagctccg ctcgcaaaga cagactacta taaacccgtc caatccttcg actgcacact
     1381 acgtgtgtgc tcaggacgaa cgggttttgt tgaatagatg agtgtaaggt gtcaccctca
     1441 cccgtaccct ctcccattaa gggagagggg aattcttagt taggctgagg aggttcacta
     1501 gaatcgtata tgagcacaca ttgtgtgcta aatggggaaa atttgttagc tgaaaataat
     1561 tattgtaagt gaaaaatcgt gtgctatagt aagtaagcac ttacttatga atagtgtgct
     1621 ggaatcgata cttagtgcag tacaagccgg tcaatacctc ccttcatacg atttccacag
     1681 tttatttttt ccggtaactt cggacgcata cacacagtta tcgatgggtt tcggcggggg
     1741 agccctctcc atagatccgg gcgcgtttta tctatgatct ctaccagaaa attatccctg
     1801 gacaaattaa gtagcctact ttatattctt agctaatatt aagtaattgg cttaatatga
     1861 gtgcgtgagg aatccgtaca ttgccggtgt ttcagaacgt aatcccgaac gtcaaccata
     1921 aatccacgag cagtaatcag agggactatc tccatgaata cttccgcgat acagccgaga
     1981 atcgcaggca tatccacagt cgttccgtct tacagcttca gtcagaaatt cgccatcgat
     2041 gcggccagga agtggctgtc gtcgggcggt gagcccgagc tctcggtatc gaggaccgtg
     2101 caggttttcg agaacgcggg cgtcgagaag aggtacagcg tcacaccggt cgaggacatg
     2161 cttagggaga aggactttcc cgagaggaac gctctctata gtaaattcgc ccggctcctc
     2221 ggcgagaaag ccctcaggga cttgttcgag aaaacgggcg tcaagcctta cgagatagac
     2281 ctcttcatta ccgtttcgtg cacgggcttc atgatcccgg cgctggacgc atacctcatg
     2341 aaccgatttc cgttcaggaa caacgtgagg cgtcttccgg tgacggagct cggatgcgcg
     2401 gcgggagtga gctcgctcat cctcgcctcc gattacatac gcgcttaccc cgaagcgaac
     2461 gtgctgatac tttcactcga gctctgcact ctcaacatgc agcctaccga caagagcgcc
     2521 gaccatataa tatcgacggc gatattcggc gacggcgccg cggcggcgat catcaccggc
     2581 agggaggacg cggccgggct tcacattctg aaaaccgaga acaggttctt ccccgggacc
     2641 ctcgaattca tggggtacga cgtggagaag accgggttcc acattttcct ttcgccgcgc
     2701 atatcgaagt tcatcgagag caacatcgca tccgagatgg agcaaatgct cggacaagcc
     2761 ggtcttaaaa tcggagacgt cgatgactgg ctcttccatc cgggcgggtc gcgcatactg
     2821 aaatctatag agaagggact acgcatcgac ccggcgaagc tcggggaaag cacggcggtg
     2881 ctgagggact acggcaatct ttcgtcggcg accatttact tcatcctcga ttcattcatg
     2941 cgcagcgaat ccaaaaagaa cggctcgtat caggtggtgg gggcggtggg gccggggttc
     3001 gggctcgacg cgattctcac gcaatggaaa taatctgaat ggagaccgaa ttatgagtat
     3061 tcacggtaca agcatagctc ccgaagggca gggcttcgat gccgccccct cgagcgaaga
     3121 cacggacgcg ctttactcga agcacgttaa tccgcagtgg gtgaagctct acaacactct
     3181 cggtatgaac gtgagatacg aatcgtgcct cgggtcggag ctcacgacgg gtgacgggag
     3241 gaggatactc gacttcctct ccgggtactg cgtctataac gccggtcaca accatccggg
     3301 catcattgaa gcgctcacag acgagctcgg gagcggaggg gcgacgatgc tacagagcca
     3361 cgtcccgggt ctcgcggcgg agctcgcgtc gcggctgtgc tcgctcgcgg gcgggcgtct
     3421 taacaaaata tttttcacca gctcggggag cgagggggtg gagacggtca taaagttctc
     3481 ccgcgcacac acgaaaaggc cgggggtgct ctactgtgaa ggggctttcc acgggctcac
     3541 gtgcggggcg ctctcgctta tggacggccc ttactggaag gaagggttcg ggccgctcct
     3601 gcccgatacg gaatccgtgc cgttcctcga cctcggagcg ctcgaagaga agctcaggag
     3661 cgggaggttc gccgcgttca tcgtcgagcc tgtgcaggcc gaggcgggaa tactcgtgcc
     3721 gccgtcgggg aggctcaagg aagcggaagc gctatgcagg aaatacggga cgcttttcgt
     3781 gctcgacgag gtgcagacgg gcatctacag gaccgggccc ttcctcgcgg cacaccactg
     3841 gggtctcgag cctgacatgg tggtcctcgc gaaggcgctc agcggcgggt tcgtcccgtg
     3901 cggcgcggtg cttatgtcgg acggtataag caggtcggtc ttcagcacgg tgacgcgtgc
     3961 tttcgtgcac gcctccacgt tcggcgagaa taacctcgcg atgcgcgccg ggctcgcgac
     4021 gctcgacgtg atgaaggaag aaagacttgg cgcgcgtgct ctctcgctcg gggagcgcct
     4081 caggaagagg ctcggagagg tatcgcgcgg ctacgagatg gtgcgcggaa taaacggcct
     4141 cgggatgctg aacggcatag agttcgccga gccgaaacgg ctcaggctca ggatacccta
     4201 cgagactttc aggaagatac acccggggat gttcggcatg atagtggtga tgcgtctttt
     4261 cagggagaag aacatactca cgcagatatg cggcaataac cacatggtgc tgaaggtagc
     4321 gccgccgctc gtcgttacgg acgctcagat agaggagttc gtcgcggggg tgagggacgt
     4381 gctcgagacc gtgcacacga agacgtcgtt ctggtcggaa gcactgctca tggcgaggcg
     4441 cgcgttaggc tcctgagccg gggacgatgc aattgttaca tcagcaacaa cagcacatca
     4501 agacggagag gaaatcatga gagtaaaatc atacgcggcc tttatattgt ccgccgcctt
     4561 tatcgtatcg tttataccca gcgcgggatt agcgtcgcag gacacggcgc cgggctcagg
     4621 gggcggggtg acgcccgacg tttacgcggg cgggcgcgtg acggagttcg aggtggtcgc
     4681 cggcaagagc tcggtcgagt tcacagggac ctcactcatg caccacttcc acggcgtatc
     4741 gcacgatccc tcgggattca ccagcgtcga tttcggaaac ccccgggcga cgacgtcggc
     4801 cgagataacc gtgcccgtgg attccataaa gggcatcgct ctcggctcgg agaaaagcga
     4861 tctttcgaag aacatccatg agaacctcga atcggagaag tacccggaga ttaagttcaa
     4921 agtgacgcag atcctgcccg aaagcacgga cgcctcagct ccatcgaaga ggagctatct
     4981 tctaaagggc gacctcacca tccataacat gacaaagccg gtcgtgctca cggccgatac
     5041 ggaaataaag gacggctacc ttcacgtgac gggcgaatac gacaacctcg acatgaccga
     5101 ttacggggtc gagccaaagc cgctctttgc cgtagtcagc gtgaacgacg tggtggacgt
     5161 gaaattcgac ctgtacgaag acctcaggga aagcgctccg gtccattcat cggtccggta
     5221 agcgtaaaac cgccaacatc aaaatatatt ctcacgagag ggtgaatatg gaaagcgatg
     5281 ttatcgtcat aggggccggg cccggaggct cggcgagcgc gatctcgttc ctgagacgcg
     5341 gtttcggagt gaccgtgctg gagaaaaggt tcatgcccag gacgaaggtg tgcggggaga
     5401 tggtggcccc gctcggcgtg aaggagctcg ggcgtctggg agtactcgcg gggatagaag
     5461 ccgcaggggc gaacagggtg aggaaagttg ttttccgctc gtccggggga gccgggacgg
     5521 agataccgtt tccggggggt gaatacgggg tcgcgatgag cagggaggcg ttcgactcga
     5581 tgctgttcgc cgaggcgcgc gaaaggggcg ctctcgggat cgaggggttc agggtcgaga
     5641 acgtggagag ggagcgcggc ggaaagttcc gcgtggaagg gacgcgcacc gagacggggg
     5701 aaagggagac ctaccgctcg cgggcggtga ttaatgcgtc gggtctcatg gggatcggat
     5761 acagggataa aaagtgcagg gagttcctct acgcgttcaa gatacacctc cgggggatta
     5821 cgtgcgggga cgcggccgag atattttttt acgacggcgg atacgggggg ctcgtcgata
     5881 tcgagggcgg tattgttaac ctcgcgtttc agatcaggaa ggaccagatg cctctcatga
     5941 aagggcatcc gctcgatatt ctcacggctg tgctgcattc gggtcatcca gtccgggaaa
     6001 agctgtccaa tgcagtgccc gtcggggagt gggaggcgat ggggagcgtg ttcgtcagaa
     6061 agcgggggtc gtttccgggg gtatggaatg tcggggactc gtccgggatc atagacccgt
     6121 tcaccgggct cgggataagc ctcgcgctcc agggagggag gtacataggg gagaacctcg
     6181 aagtcgggag ggtgagggat acctacgatc tcaagaggag cctaaacagg acgaggctca
     6241 tgacggcggc gcttctcagg aaggctcttt ttcgtccgcg cctctgtcac atgataatga
     6301 agcatgcgtc catctccccg atgctcggga tgatagtgct cgggctgctg catacgtgag
     6361 gcgggaatac ttctttgtct agatacaaag acgcacacta gtgtcattta tgaaaacact
     6421 tggaaaggag aaaggcagtt gttttatcct tcttttcctt gatgaaaaga agcaaaaatc
     6481 aaccgactga acataatttg ctaaaaatct catattaagg cgaaaatgtt ttaattccac
     6541 cccaccccta aaacattttt acgcctaaat actctatttt cttaacgcaa attctgtaat
     6601 gtcggaaata catcgtcctt cgacaggctc aggacggacg ggatttatta tcgcgactgg
     6661 aagccgctcc tacgttgaat taagaatagc aagtgacggt tggaaccggc gcgggtcgga
     6721 tccgtaaggc ggtgcggtgc gtatcaagtg tgacggactc tatgccgggg gcttgtccgc
     6781 ccgcgcctga atacattaga atattcagcg ggagcggctc cggatcggtt ctcagccgga
     6841 ggccggaata gatatgacta aaccgatgaa gactgcggcc atgaagaagg gcctccatcc
     6901 gggcgagctc atacacgtag gcgagaggaa ggtcgagcac gtcaggatga cggtgatgga
     6961 ctacgaccgg gatatgttca tcgagcggga atacaagctc gtggaggagt gcctggagta
     7021 tgcggacagg gacacggtga cgtggatcaa cgtgaacggc cttcacgata cgcagataat
     7081 tagcagtata ggcgagcact tcggcattca cccgctcgtg atcgaagaca ttctcgacac
     7141 cgagcagcgg cccgagctcg acgactacct cgattacata tccttaatgc tcaagatgat
     7201 actcgtcgaa aaggacacag gcaggataga cgccgagcag ataagcattc tcctctgcag
     7261 gaacaccgta atctcctttc aggaagggga tggcgatgta ttcgaaccca taagggagag
     7321 gataaggacg ggaaaggggc gcatacggga gtcgggcgcc gattacctga tgtacatcct
     7381 gacagatgct gtcgtcgata actatttcgt cgctctcgaa gagatcgggg agctgatagg
     7441 ggctatcgaa gacgaagttc tggagaaacc gaacgcagag acgctgatgg agatttacga
     7501 gctcaagagg gagctcgtgc tgttgaggaa atatacgttc ccgctgaggg agctagtcca
     7561 taagttacag atatcggaat cgccgctcat agacccgagc acgaagcttt acgtgcgcga
     7621 cctttacgat cacgcgatgc agattatgga cacgaccgag tcgtataggg acatgataac
     7681 cggtatgcta gagacgtatc tctcgctatc gagcaaccgg atgaacgagg tgatgaaggt
     7741 gctgaccgtg atggcgacga tattcatacc gctcacgttc atagcgggca tttacgggat
     7801 gaacttcgag aatatgcccg agcttcacca ccatctgggg tatccgggcg tgatgctgct
     7861 catgctgata gtagggctcg ggatggtgta ttacttcagg aggaggaagt ggatttgagg
     7921 atgcgtagtg ttggatttta gaagaacaag gaaaggagaa agacaatata ttttattctt
     7981 cttttccttg atgaaaagaa gcaaaaatca accgacagaa cagaatttac taaaaatctc
     8041 atgtttacgc taaaacattt ttattccacc cgatagggcg atgtagtctg atgatgtgcg
     8101 atgtaaaatg cgccgtttgg ataaggtaag gcgtccgaat gcgcgctagc ttgcattcgt
     8161 acccaaaaat gttttttgac gcgtaaacaa tcgattttct ttagccgatt tgacagtaga
     8221 aaataacgga gcccagaaag tggcacattg tctgttagga tagagcgacc gattcctcgc
     8281 ttgctcgaaa tcgtttccgt aatgtcgggg aagataaata cagaattaac tgcgaaagac
     8341 gagattgccg cgcggagcct gtcctgagcg tagttgaagg gcttgcaatg acagatgatg
     8401 tatccttcaa ctatacccgc ctagatgttc gcacccgcct gaaaccgttg tataatgttc
     8461 cctatattaa acaatcaaag tcagggagcg ggttatgtat aaggacaacg agaccgtaag
     8521 cgtgatggtg gagatcacca aggggtcgag gaacaagtac gaatacgata aggtgaagaa
     8581 gcggataaag ttcgacaggc ttctctacgc ttcggtccat tatccgagtg attacggttt
     8641 catcgaggac actctgggcg gggacggaga cccgctcgac gcgctcgtgc tgctatggga
     8701 gccgacgttc cccgggtgca tcatactgtc gaagcccata gggatgttca tgatggggga
     8761 cgagaagggg cccgacgaga agatactctg cgtgcctgtg gatgacccgc actggaacca
     8821 catcgagaag ctgaccgaga taccgcccca cctgctccgg gagatagagc atttcttcgc
     8881 gtcgtacaag acgctcgaga agaagaacgt gcacgtggag gggtggagga acaaggaggc
     8941 cgcgcttcag acgatccagg aggcgcggga taggtacaag cataagggga agaagaaaaa
     9001 gaaggcaaag aaggctgttg attggctgtg atgctgcagg tgaatta
//
LOCUS       DASSJH010000323         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1589984, whole genome shotgun sequence.
ACCESSION   DASSJH010000323 DASSJH010000000
VERSION     DASSJH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589984"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1090)
                     /gene="surE"
                     /locus_tag="VFJ67_07050"
     CDS             complement(329..1090)
                     /gene="surE"
                     /locus_tag="VFJ67_07050"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET7290183.1"
                     /translation="MLPTILISNDDGINSEGLHKLHEALKPLGEVFIVAPDRDQSAVS
                     HSLSLYRPLRIEKISDNVYTVDGTPTDCINLAVNGILKDVKLDLVVSGINKGENLGDD
                     ITYSGTVSAAMEGALLGVNSIAVSLATKVNFNFEAPSHYSHMIARYVLEKKLPEDTVL
                     NINIPNLPVDEIKGVLVTRQGKRVYGEPVVEKIDPRGKKYYWIGGYELGSVDIENSDI
                     TAVRDGYVSVTPISLDLTNYGYLDKLRSDLSGKQR"
     gene            complement(1202..1278)
                     /locus_tag="VFJ67_07055"
     tRNA            complement(1202..1278)
                     /locus_tag="VFJ67_07055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1242..1244),aa:Pro,seq:ggg)
     gene            complement(1326..1670)
                     /locus_tag="VFJ67_07060"
     CDS             complement(1326..1670)
                     /locus_tag="VFJ67_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7290184.1"
                     /translation="MLQNKAEVSLPDKIYFRIGEVSEFTGIKPYVLRYWESEFEEITP
                     IRRKSQRLYDREAIHTIIKIKNMLYEQSFTIAGAKKKLKEEANQKRVSTKVNVLLHEV
                     LGELKSIKQNLG"
     gene            complement(1787..>2534)
                     /gene="trmFO"
                     /locus_tag="VFJ67_07065"
     CDS             complement(1787..>2534)
                     /gene="trmFO"
                     /locus_tag="VFJ67_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET7290185.1"
                     /translation="NCPLNKEEYYALVEEISGAEKVETRDFEKGIYFESCLPVEEIVE
                     RGRDTLRFGPARPVGLRDPRTGSMPYAVLQLRAENKERSMYNMVGFQTKLRYPEQRRI
                     FRMIPGLENAEFMRYGSVHRNTYIDSPRLLRGTLQLIKSDKIFFAGQIVGVEGYIESA
                     AMGIIAGINAARVARGESPVVLPPETATGSLLRYISDGGIKNFQPMNINFGLFPPSGE
                     KIQKSRRKSCIAERALRAMSGFGPFGQASD"
BASE COUNT          553 a          689 c          613 g          679 t
ORIGIN      
        1 ggagctccgc gtagaggcac gggagcacta gcgctatcgg acgcgtctcg cagaactcaa
       61 gtagttgctc ttccaacaat cctacattat tcctgttgag cctgtggagc gttgaaaacg
      121 attcagtctg atagaaatca cccatgtctt acacctcccc acccaacgaa acatgtatga
      181 taacacacac gaatacgaca tcctaacgtt tttttcactg gcgagaggag ctgaatatac
      241 gggtcaaaga cgctgaatat gaggagagtt cacttgctgc cgggcaatcg aatcggcccg
      301 caacgatgca tattcggatt gtttggattt atcgctgttt gccggataga tcggacctca
      361 gtttgtcgag gtatccgtag ttcgtgaggt cgaggcttat aggtgttacc gaaacgtaac
      421 cgtccctcac ggcggttatg tcggaatttt ctatatcgac ggagccgagc tcgtatccgc
      481 ctatccagta atatttcttg ccgcgcggat ctatcttttc cacgacaggc tctccgtaca
      541 cccgctttcc ctgtctcgta acgaggacgc ccttgatctc gtccacgggg aggttgggga
      601 tgtttatgtt gagcaccgtg tcctcgggga gctttttttc gagcacatac ctggctatca
      661 tgtgagagta atgtgaaggc gcttcgaaat tgaaattgac ttttgtcgca agcgatacgg
      721 ctatggaatt gacgccgagc agagctccct ccatggctgc gctcacggtg cccgaatacg
      781 ttatatcgtc gcccaggttt tcacccttgt tgatgccgga gacgacgagg tcgagcttca
      841 cgtccttgag tatcccgttc acggcgaggt taatgcagtc ggtaggggtc ccgtccaccg
      901 tgtaaacatt atccgatatc ttctctatac ggagcgggcg gtagaggctg agcgaatggc
      961 tgacggcgct ctggtccctg tccggggcga ctatgaacac ctcgcccagc ggtttaaggg
     1021 cctcgtggag cttgtgcagc ccttccgagt ttatcccgtc gtcgttcgat atcagtatgg
     1081 tcggaagcat gtttttctta ccgatggaaa ggaatcgccc ggtcaggcaa tagaaaatac
     1141 acaattgaca gcacccggca agttttttca ctgcattcga ctaagatcgt atcctgatga
     1201 atggtcgggg tggccggaat cgaaccggcg accctcggca ccccatgccg atgcgctacc
     1261 aagctgcgct acaccccgtt tgagtgcgat tgaaaatttt atggaaaatg ggggaggcga
     1321 acagcttaac ccaggttctg cttaatggat ttcagctcgc ccaatacttc atgaagcaaa
     1381 acgtttacct tggtgctgac ccgcttctga ttggcttctt ctttaagctt tttcttggcg
     1441 cccgcgattg taaaactctg ctcataaagc atatttttaa ttttaattat cgtgtgtatg
     1501 gcttcacggt cataaagtct ctgagacttt ctccttatcg gggttatctc ttcgaactcg
     1561 ctctcccagt acctgagcac gtacggcttg ataccggtga actcactcac ttccccgatc
     1621 cggaaatata tcttatccgg aagactcact tcagctttgt tctgcaacat aatttaaaaa
     1681 ttagaccact ttggtttaat tgtcaagtat aaatgattag ccattcccta tgcttatata
     1741 ataatattaa agttataaaa tccgcatgca tacatacccg tctctttcaa tcggaggcct
     1801 gcccgaacgg gccgaacccg gacatggcac gcagcgctct ttcggcgatg cagctcttcc
     1861 tccgggattt ctggattttc tctcccgagg gcgggaacaa tccgaaatta atgttcatgg
     1921 gctgaaaatt tttaatgccc ccgtccgata tatatctgag gagcgatccg gtcgccgttt
     1981 cgggcgggag gacgacgggg ctttcgcccc tggctactct cgcggcgttt atacccgcta
     2041 taatacccat agcggccgat tcgatataac cttcgacccc cacgatctgt cccgcgaaga
     2101 atatcttatc gctttttatc agctgaagcg tcccccgaag gagtctcggg gagtctatat
     2161 acgtgttcct gtggacgctc ccgtatctca tgaactctgc attttcgagc cccggtatca
     2221 tcctgaatat gcggcgctgc tccgggtacc tgagcttggt ctgaaaaccg accatgttgt
     2281 acatcgagcg ctccttgttc tccgctctca gctgtagcac ggcgtacggc atgctgcccg
     2341 tgcgcgggtc cctgagaccc acaggcctcg ctggcccgaa cctgagcgta tccctgcccc
     2401 tctccactat ctcctcgaca ggcaggcagc tctcgaagta aatccccttt tcaaaatccc
     2461 tcgtttcgac cttctccgca ccggatatct cttccacgag cgcatagtat tcctctttat
     2521 tgagcggaca gttg
//
LOCUS       DASSJH010000324         5830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1590141, whole genome shotgun sequence.
ACCESSION   DASSJH010000324 DASSJH010000000
VERSION     DASSJH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590141"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VFJ67_07070"
     CDS             <1..1210
                     /locus_tag="VFJ67_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HET7290186.1"
                     /translation="KARVRKLITDSLRYWVREMHVDGFRFDLAVSLFRDKPDYSGKHI
                     LFDMIKEDPVLSKVKLIAEPWDIGPGGYHAGGFPEPWSEWNDRYRNAVRRFWRGDHGQ
                     TGDLAYRISGSSDLYRSRPRGPYTGINYVTSHDGFTLNDLVTYEKKHNQANLENNKDG
                     TDSNFSFNFGVEGPSDDPVVESSREKQKRNLIATLFLSQGIPMFTAGDEIGRTQRGNN
                     NAYCQDNYISWMNWTLGQRQKELLEFTRLMINFRERHPILNQGKFFHGNIVRYNGFKD
                     LIWFLKDGNEMTHAEWHNGNLLSIGLIKSGDNLEDIRRKRKPGYENTLMILLNADPAE
                     AEFTIPSFGVAEEWQVMIDTSSAGSPKGPRRVSSGAKHVMTGRSLKVLKPIYTKKTER
                     GIPTVKKTNR"
     gene            1207..2709
                     /gene="malQ"
                     /locus_tag="VFJ67_07075"
     CDS             1207..2709
                     /gene="malQ"
                     /locus_tag="VFJ67_07075"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7290187.1"
                     /translation="MSKRASGILMHITSLPSRFGIGDMGPEAYRFADFLRDSSQSYWQ
                     VLPLNPTDLSFGNSPYSGPSSYAGNPLLISPELMMEAGYLSADDLSIKPFPGRGKVDY
                     SLVSEYKTALFFRAFANAASSLPVDRGFNEFARENEDWLDDYALYASVKRILGSGTWR
                     EFPAELRDRDGESLDAWREKAGSEILYHKFLQYIFFSQWNSLKSYVRERGIRFIGDIA
                     YYINYDSSEVWSSPGLFKLNEDKKPGFVAGVPPDYFSKTGQLWGNPVYDWEAIKERGY
                     EWWIRRIGHNLKLFDSLRLDHFRGFVSYWEVKAGKKTALDGKWVSLDAESFFDTLFSH
                     FDKSRFIAEDLGYITPDVKEIIKKYGLPGMKILLFAFGNDFPYGDYLPKNFSSGNCVV
                     YTGTHDNNTVMGWWKKDAGSAEKHRFFKYIGKVIDEGDINREFIRLAMMSIADTAIFP
                     MQDLLGLGAGAKMNRPSTKSGNWEWRLRHGYYRSELINKLTEITRAGDRG"
     gene            2811..4286
                     /gene="glgA"
                     /locus_tag="VFJ67_07080"
     CDS             2811..4286
                     /gene="glgA"
                     /locus_tag="VFJ67_07080"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET7290188.1"
                     /translation="MIKVLFLASEMEPLAKTGGLADVIGVLPKKLNEIGCDTRVILPY
                     YREVKKNLRQLKLKVKNVSKEVLVSIDWLAYKGTIKETNVEGVTVYLLCNDEFYDRDS
                     LYTTPNGDYQDNDIRFGFYSLGALEVAKALNFKPDIIHCNDWQSAMAPIALRWKKHYN
                     NDQFFNDSKIVFTIHNISYQGIYMPDLLDKFGLSHSLFNTEHLEFYGRVNLLKGGIIT
                     SDLVTTVSPTYAKEIRTREYGYGLDGVLRVISGEGKLVGVLNGIDYDDWDPKRDKELF
                     FNFSSDDLDGKYRNKSKLKSILGFEANGHKPLIGVISRLALQKGVDLVVRSLNSVLDL
                     DVDLVILGKGDKRYEKLLRDVSADYRGNFKAIIGYDEAKARRIYAGCDMFLMPSRFEP
                     CGLGQLISLRYGTIPIVRATGGLLDTIIDYNENEVAGNGFVFNHFSERDMVDAIERAL
                     DVYRNREEWTNLIKTGMRMDYSWKKSSKVYLKHYKTLLDRN"
     gene            4517..5770
                     /locus_tag="VFJ67_07085"
     CDS             4517..5770
                     /locus_tag="VFJ67_07085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7290189.1"
                     /translation="MRKYESALRQNVLWWVDEIKSVDILVGIPCYNCEDSIGFVVEQV
                     AKGLSQYFPDYKTAIFISDGGSLDDTRENAYQAKIPENIYRRVTIYRGFPGKGTSFRA
                     VFELAIMLKVKAIAVFDADLRSITPEWVKFIVEPILDGSAGFVAPYYRRHKFDGTITN
                     QIVYPMTRALYGIDVRQPIGGDFGFCPELAAFYVKEDVWDTDVARFGIDIWMTTCAVN
                     EGFNIVQTYLGTKIHGAKDPASDLGPMFRQVVSTVFYLMSKYEHNWHRENPFQAIQIK
                     NKIEEEPKLEAISVSMNDLREEFVEGYMHFRPMYTEILNDDNLERLDDIYNSWLKNTD
                     AEFDAVLWSKILYDFAYIYQQWQRNKRRLVDIITPLYFGRVKSYCQQVMNLTSDEAEE
                     VVQNQAKIFEENKSYLLKRFEIWKD"
BASE COUNT         1626 a         1403 c         1560 g         1241 t
ORIGIN      
        1 gaaagcgcgc gtaaggaaac tgataaccga cagcctccgg tactgggtca gggaaatgca
       61 tgtcgacgga ttcaggttcg acctcgcggt ttcactcttc agggataaac ccgattacag
      121 cggtaaacac attctcttcg acatgataaa agaagacccg gtattatcga aggtcaagct
      181 catagcggaa ccctgggaca tcggacccgg agggtaccac gcaggcggtt tccccgagcc
      241 gtggtccgag tggaacgaca ggtacaggaa tgcggtcagg cggttctggc ggggcgacca
      301 cgggcagacg ggcgacctgg cgtacaggat aagcgggtcg agcgatttat acagatcgag
      361 accgaggggg ccgtatacag gaatcaatta cgtcacgtct cacgacgggt tcacgctcaa
      421 cgacctcgta acatacgaga aaaaacataa ccaggcgaat ctggagaata ataaagacgg
      481 cacagactca aacttcagtt ttaatttcgg cgtcgagggg ccctccgatg accctgtggt
      541 tgaaagcagc agggaaaagc agaagcgcaa cctcatcgcc acactctttt tatcccaggg
      601 cataccgatg ttcaccgcag gggacgagat cggcaggacg cagcggggca acaacaacgc
      661 ctattgccag gacaactaca tttcctggat gaactggacg ctcggacaga ggcagaagga
      721 gctcctcgaa tttacaaggc tgatgatcaa cttcagggaa agacacccga tactgaacca
      781 ggggaaattt tttcacggca acatcgtcag atacaacggg ttcaaagacc tgatatggtt
      841 ccttaaagac ggaaacgaga tgacgcacgc ggaatggcac aacgggaacc tcctttccat
      901 cggtttaata aaatcgggcg acaacctcga ggacatcagg aggaagagga aacccggata
      961 cgagaacacc ctcatgatcc ttcttaatgc ggaccccgca gaggcagagt tcacgatacc
     1021 gtcgttcggc gtcgcagagg aatggcaggt catgatcgat acctcatcgg cagggagccc
     1081 gaagggcccg cgaagggtct cgtcaggagc caagcacgta atgaccggaa ggtcgctcaa
     1141 ggtgctaaag ccgatctata cgaaaaagac cgaacgtgga atccctacag ttaaaaagac
     1201 gaatcgatga gcaagagagc gagcggcata ttaatgcata taacatccct gccgtcgcgt
     1261 ttcggcatcg gggacatggg ccccgaagcc tacaggttcg ctgatttcct ccgcgattcg
     1321 agtcagtctt actggcaggt cctgccgctc aacccgaccg atttatcttt cggcaattcc
     1381 ccatatagcg gcccttcgtc ctacgccggg aaccccctcc tgataagccc cgagcttatg
     1441 atggaagcgg gttatttatc agctgacgat ctgagcatca aaccctttcc cggaaggggg
     1501 aaggtcgatt acagtttggt ttcagaatat aaaaccgcgc tcttcttccg cgctttcgcg
     1561 aacgctgcgt cctcgctgcc ggtggacaga ggattcaatg agttcgcgag agaaaacgaa
     1621 gactggctcg acgattacgc cctctacgcg tcggtaaaga ggattctggg gagcggtacg
     1681 tggagagaat tccccgcgga gctgagggac agggacgggg agtcgctcga tgcgtggaga
     1741 gaaaaagctg gcagtgagat cctatatcac aagttcctgc aatacatctt cttcagccag
     1801 tggaattcgc tcaagagcta cgtccgggag aggggcatcc ggttcatagg agatatcgcc
     1861 tactacataa attacgacag ctcagaagtg tggagcagcc ctgggctctt caagctcaac
     1921 gaagataaga aaccgggatt cgtcgccggg gtcccgcccg attatttcag caagaccgga
     1981 cagctctggg ggaacccggt atacgactgg gaggccataa aagagagagg gtacgagtgg
     2041 tggataaggc ggatcggtca caatttgaag ctttttgact ccctcaggct cgaccatttc
     2101 aggggcttcg tctcgtactg ggaggtgaaa gcggggaaaa agacagcgct cgacggcaag
     2161 tgggtcagcc tcgacgccga aagtttcttc gatacattat tctcgcattt cgacaaatcg
     2221 aggttcatcg ccgaggacct cggctatata acaccggacg tgaaagaaat tataaaaaaa
     2281 tacggactgc ccggaatgaa gatactgctc ttcgccttcg ggaacgattt cccgtatggc
     2341 gactacctgc ccaaaaactt cagcagcgga aattgtgtcg tatacacggg cactcacgat
     2401 aacaataccg tcatgggctg gtggaagaag gatgcgggga gtgctgaaaa acacaggttt
     2461 ttcaagtaca tcggcaaggt catcgacgag ggcgacataa accgggaatt cataaggttg
     2521 gctatgatgt cgattgccga tacggcgata ttccctatgc aggatctgct cggcctcggc
     2581 gcgggggcaa aaatgaacag accctccact aaatcgggca actgggaatg gagattaaga
     2641 cacggttatt acagaagtga attgataaat aaattgacgg aaataacccg agccggcgat
     2701 aggggataaa tttcaagtta tgtataattg tcctaaatac attccctaaa tttccattat
     2761 aattattgga acctcaagcg ggtgggtaga ataaacgagg gggttgttaa ttgatcaagg
     2821 ttttattcct ggcctctgaa atggagcctt tggctaaaac gggcgggctt gctgacgtca
     2881 taggtgtact acccaaaaaa ttgaacgaga tcggctgcga cacaagggta attctccctt
     2941 attacaggga agttaagaaa aaccttcgtc agcttaaact taaagtaaag aacgtatcta
     3001 aggaagttct ggtcagcatc gactggctcg cgtataaagg tacgatcaaa gaaacgaacg
     3061 tagagggcgt aacggtttac ctgctgtgca atgacgaatt ctatgaccgc gactccctat
     3121 acaccacccc aaacggcgat taccaggaca atgacattag gttcggattc tattctctgg
     3181 gagcgctcga ggttgcgaag gctctcaatt tcaaaccgga catcattcac tgcaacgact
     3241 ggcaatcggc aatggccccg atagcgctga gatggaaaaa gcactacaac aacgatcagt
     3301 ttttcaatga ttcgaaaatc gttttcacga ttcacaacat atcctatcag ggaatataca
     3361 tgcccgacct gctggacaaa ttcgggttaa gccactcgct gttcaatacc gagcatctag
     3421 agttctacgg gagggtcaac ctcctgaagg gcggcatcat cacctccgat ctggttacca
     3481 ctgtaagccc gacgtatgcg aaggagataa ggacgagaga atacggatac ggtctcgacg
     3541 gcgtgctccg cgtaatctca ggcgaaggga agctggtcgg cgtgcttaac ggcatcgatt
     3601 acgatgattg ggaccccaag agggacaagg agctcttctt taatttcagc agcgacgacc
     3661 tcgacgggaa atatcggaac aaatcgaaac tgaagagcat tctcgggttc gaggccaacg
     3721 gacacaagcc tcttatcggc gttatctccc ggcttgccct tcagaaaggc gtcgacttgg
     3781 tcgtcaggtc gctgaacagc gtgctcgatc tggacgtcga cctcgttata ctcggaaagg
     3841 gcgataagag gtacgagaaa ttgctcaggg acgtttcggc ggattacagg gggaatttca
     3901 aggcgattat cggctatgac gaggctaagg cgaggcgtat ttatgccgga tgcgatatgt
     3961 tcctcatgcc gtcgcggttc gagccgtgcg ggctcggcca gctgatatct ctcaggtacg
     4021 ggaccatacc catcgtgcgg gcgacgggag ggcttctcga tacgattatc gattacaacg
     4081 aaaacgaagt ggccgggaac ggcttcgtat tcaaccattt cagcgaacgc gacatggttg
     4141 acgctataga aagggcttta gatgtatata gaaaccgcga agaatggacc aatttgatca
     4201 aaacggggat gagaatggat tattcgtgga aaaaatcgag caaggtttac ttgaagcact
     4261 acaagacgct cctcgacagg aattaatctt taaaccccta tcttaaaaac actttatccg
     4321 ccaaaaacaa tctgaaatca gtaaaatttc aataatatca tgtttgccgc tgcccttttc
     4381 cggtctcatt agagccacgc aagcccccgt tttcagccat gatagctaaa ggcgagaagc
     4441 cgattatttt tttcagcatg atacgattca gtgttatatt tatgtttttt aatcgattgt
     4501 aagggataat aacagcatgc gaaaatatga atctgctctg agacagaatg ttctctggtg
     4561 ggtggacgag ataaaatcgg tggatattct ggtcggaata ccgtgctata actgcgaaga
     4621 ctcgatagga ttcgtcgtcg aacaggtagc caaaggttta tcccagtact tcccggacta
     4681 taaaacggcc atcttcataa gcgacggagg ttctctcgac gatacgcggg agaacgcgta
     4741 ccaggcgaag ataccagaaa acatatacag gagagtaacg atatacagag gtttccccgg
     4801 aaagggcaca tcgttcagag ccgttttcga gcttgcgatc atgctcaagg taaaggcaat
     4861 cgctgtattc gacgcggacc ttcggagtat tacccccgag tgggtcaaat tcatagtaga
     4921 gcccattctc gacggttcgg ccgggtttgt agccccttac tacagaagac ataaattcga
     4981 cggcactatc accaaccaga tcgtataccc catgaccagg gccctttacg gtatcgacgt
     5041 caggcagccc ataggcggtg atttcggttt ctgccccgag ctcgccgcgt tttatgtcaa
     5101 ggaagacgtc tgggacacgg acgtggcccg gttcgggata gatatatgga tgacgacctg
     5161 cgcggtaaac gaggggttca acatcgtcca aacctacctg ggcacaaaga tacacggcgc
     5221 caaggacccg gcctccgatc tcggaccgat gttcagacag gtagtgagca ctgtctttta
     5281 tctcatgagc aaatacgagc acaactggca cagggagaat ccgttccagg cgatccagat
     5341 aaaaaacaaa atcgaggagg agcccaagct tgaggcgata tccgtcagca tgaacgacct
     5401 ccgcgaagaa ttcgtcgagg gatacatgca cttccgtcct atgtacacgg agattctgaa
     5461 cgacgataac ctagagagac tggacgacat atacaacagc tggctaaaaa atacggacgc
     5521 ggagttcgac gctgttctct ggagcaagat cctttatgac ttcgcgtaca tctatcagca
     5581 gtggcagcga aacaagagaa ggctcgtgga tatcattacg cctctctatt tcggaagagt
     5641 aaagagctac tgtcagcagg ttatgaacct gacgagcgac gaagcggagg aggtcgttca
     5701 gaaccaggcc aagatcttcg aggaaaataa atcctacctt ctcaagagat tcgaaatctg
     5761 gaaagactga ttccggcacg gaattcacgc ttcgggaata tcggcacgca gttacttcgg
     5821 gaacggcccg
//
LOCUS       DASSJH010000325         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1047263, whole genome shotgun sequence.
ACCESSION   DASSJH010000325 DASSJH010000000
VERSION     DASSJH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047263"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFJ67_07090"
     CDS             complement(<1..433)
                     /locus_tag="VFJ67_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7290190.1"
                     /translation="MTKNAGSSLQAELEDSLRRKIEGEVRFDNLTRTLYSTDASNYEI
                     EPVGVVIPRTEADVSAAVETAFGLGVAILPRGGGTSLAGQAVGHALVIDFSKHMNGVI
                     DIDPETKTVRVQPGIYIEQLNRRLKPSGLMFGPDPSTVRIAT"
     gene            614..1267
                     /gene="cofC"
                     /locus_tag="VFJ67_07095"
     CDS             614..1267
                     /gene="cofC"
                     /locus_tag="VFJ67_07095"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HET7290191.1"
                     /translation="MLFAIVPVKDLSKAKESLSSLLPQDVRTELAYAMLEDVLSALNG
                     ATLIDRIFIVTMDRKAMGIAGEIGIEIIEETEQKGESDSVDRASIVCKEMGADSVLVI
                     PGDAPLIRSEDIDFIAGKGHGSPSVILVPARDRMGTNAILRTPPDAIPSRFGHDSFRK
                     HMEEAGKKGIAIECYENERVGLDIDHPDDLKLFASKESDTRTYRLLLSKNILGKIRA"
     gene            complement(1291..2727)
                     /locus_tag="VFJ67_07100"
     CDS             complement(1291..2727)
                     /locus_tag="VFJ67_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7290192.1"
                     /translation="MSREFGFLVNGEWIKGKKKREIKSPYNGEVAGIINIPDRETALS
                     SIVFAERAFSKWRESPSYERSRILRKIAEGIAARKEEFARTLVLEGGKPLKTARVEVD
                     RAVMTFSVAAEEANRFREGELVPIDIAPGNDGMFGIARRFPLGVVVGISPFNFPLNLV
                     AHKVAPSIASGNAMILKPASQTPLSALILGEIVTESGLPGGLLNILPLPGADVEPVID
                     DERIKKVSFTGSDAIGWELKKKYPKKKVTLELGGNAATIIDEDPPDLDFAAGRNAWGA
                     FYQAGQSCISVQRMYVHEKTFDKFISRFVEAARKLKLGDPMLEDTDVGPVIDTQSAAR
                     IMKWIEDALAKGAKLLSGGNRDGSLIEPTVLTDVPPGCDVSKNEVFGPVVHVERYRDF
                     DDALKAVNDSRYGLQAGVFTKDMKKAFKAYSALEVGGVVVNDYPSFRVENMPYGGVKD
                     SGVGREGVRYAIEEMTELKLMVVNLNYK"
     gene            complement(2847..>3548)
                     /locus_tag="VFJ67_07105"
     CDS             complement(2847..>3548)
                     /locus_tag="VFJ67_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HET7290193.1"
                     /translation="LNKRALNKELETTAISAHGYLKVKDDYRIMSCGASMGEGYGPIV
                     VSCVPTEDLRGKEIGVPGKLTTAFLLLSIYADGFIPVEMPFDEIMPAVASGDIETGLI
                     IHEGQLTYKDYDLELVLDLGVQWQIDTTLPLPLGLNVVRRDMGEELQKEVHRVHKESI
                     EYGLAHKKEALEYAMKYGRGIGEDVGEKFVLMYVNKYTQDLGGKGKAALEFLFNKAYD
                     KGIIKEEPRLDILEG"
BASE COUNT          707 a         1036 c          923 g          882 t
ORIGIN      
        1 cggttgcgat cctcacagtc gaagggtcgg gcccgaacat cagccctgaa ggtttaagcc
       61 tccggttgag ctgctctata tatattccgg gctgcacccg tacggttttg gtttcgggat
      121 cgatgtcgat gacgccgttc atatgcttcg agaaatcgat gactagagcg tggccgaccg
      181 cctgtccggc gaggctcgtt ccgccgcccc ggggcagtat agcgacgccg agtccgaacg
      241 cggtctcgac ggctgctgaa acgtccgcct cagtgcgcgg tataacgact cccacgggct
      301 ctatctcgta attgctcgcg tccgtgctgt agagggtcct ggtcaggttg tcgaagcgta
      361 cctctccctc gatcttcctt ctaagcgagt cttcgagctc cgcctgcagg ctgctcccgg
      421 cgtttttcgt cataagtccg tgtagtataa cttgcaaatg cgcggaacag ggaatccggg
      481 ccggtgccac ccccggtcaa aacagcctgc attacgctta ttacggaatt cgggaaaata
      541 ctggtatcat aaactgattt ttacaggcgt gtctgagagc gcgccttcaa cagacgagca
      601 caaaagaatt catatgctgt ttgctatagt tcccgtaaaa gacctctcga aagcgaagga
      661 aagtctttcg tccctcctgc cccaggacgt gaggacggag ctcgcctacg ctatgctcga
      721 ggacgtgctt tcagctctca atggcgcgac tctcatcgac aggattttca tcgtcacaat
      781 ggaccggaaa gcgatgggga tcgccgggga gataggaata gagattatag aagaaactga
      841 acagaaggga gaaagcgact cggtcgaccg ggcgtccatc gtctgtaagg aaatgggggc
      901 ggattcggtg ctcgtcatac cgggcgacgc cccgttaata aggtcggagg acatcgactt
      961 catcgctggg aagggacacg gctctccctc cgtgatactc gtccctgcgc gggacaggat
     1021 ggggacgaac gcaatactca ggacaccgcc cgacgcgata ccttcgaggt tcggccacga
     1081 cagctttagg aaacacatgg aagaagccgg gaagaaggga atcgcgatcg agtgctacga
     1141 gaacgagagg gtggggctcg atatcgacca tccggacgat ctcaaattat tcgcctcgaa
     1201 agagagcgac accaggacat atcgattact tttgagtaag aatatactcg gcaagatcag
     1261 ggcgtaacaa gtgagcggct tttgcgttca tcacttgtag ttcagattga cgaccattag
     1321 ttttagttct gtcatctcct ctatcgcgta cctcacccct tctctcccga cgcccgagtc
     1381 ttttaccccg ccgtagggca tgttctctac cctgaagctc ggataatcgt tgactacaac
     1441 gcctcccact tcaagcgcgc tgtaggcctt gaaagccttc ttcatgtcct tcgtgaacac
     1501 cccggcctgg agtccgtaac gtgaatcgtt cacggctttg agagcgtcgt cgaaatccct
     1561 gtatctctcc acgtgcacca cgggcccgaa cacctcgttc ttgctgacgt cgcatccggg
     1621 cggcacgtcc gtaagtaccg tgggctcgat gagcgagccg tccctgtttc cgcccgaaag
     1681 cagctttgcc cctttcgcga gagcgtcctc gatccatttc atgatcctgg ccgcggactg
     1741 ggtgtctatg acgggcccca cgtccgtgtc ttcgagcatc gggtcgccca gcttcagttt
     1801 tctcgccgct tcgacgaacc tgcttatgaa tttatcgaaa gtcttctcgt gcacatacat
     1861 cctctggacc gatatacagc tctggccggc ctggtaaaaa gcgccccatg cgttcctgcc
     1921 cgcggcaaaa tcgaggtcgg gcgggtcttc gtcgataatc gtcgcggcat taccgccgag
     1981 ctcgagcgta acttttttct ttgggtattt cttcttcagc tcccagccta tcgcgtcgct
     2041 ccctgtgaag gacacctttt ttatcctttc gtcgtctatc acgggctcca catcggctcc
     2101 aggcagaggc agaatgttga gcagtccgcc ggggagtcct gactccgtga cgatctcccc
     2161 gagtatcagg gccgagagcg gcgtctgtga agcgggcttg agtatcatcg cgttccccga
     2221 ggctatcgag ggggcaactt tgtgtgcgac taggttgagc ggaaaattaa acggggatat
     2281 tccgacgacg actccgagag ggaacctcct cgctataccg aacatgccgt cattgcctgg
     2341 cgcgatgtct atggggacga gctctccttc ccggaacctg ttcgcctcct ccgcggcgac
     2401 ggagaacgtc atcacggcgc ggtcaacctc gaccctcgct gttttgagag gcttaccgcc
     2461 ttcgaggacg agcgttcttg cgaattcctc cttcctcgcg gctatcccct ccgctatctt
     2521 cctgagaatg cggctcctct cgtagctcgg gctctccctc catttcgaga acgctctctc
     2581 tgcaaacact atcgagctca aagccgtctc gcggtcgggg atattgatta ttcccgcgac
     2641 ctctccgtta taagggcttt ttatctccct tttctttttc ccctttatcc actcgccgtt
     2701 tacgaggaat ccgaattccc tggacatcac accgctcctg tcgtgtgttt tgtgttgtcg
     2761 tttattataa ggctatatac agcttaccat accggggatg agaattttgg acggcagctg
     2821 ttccagggag ggagttcttt taaaatctat ccttcgagta tatcgagcct cggctcttcc
     2881 tttattattc ccttatcgta agccttgtta aaaagaaact cgagcgcggc cttccctttt
     2941 cctccgaggt cctgcgtgta tttattgaca tacatcagga cgaatttctc ccccacgtcc
     3001 tcgccgatgc cccggccgta tttcatcgcg tactctagcg cctccttttt gtgtgcgagt
     3061 ccgtattcga tgctctcttt gtgcaccctg tggacctctt tctgaagctc ctcacccatg
     3121 tcccgcctta cgacgttaag cccgagaggc aggggaagcg ttgtgtctat ctgccactgc
     3181 acgcccagat cgagcacgag ctcgaggtca taatccttgt atgtaagctg cccctcgtgg
     3241 attataaggc ccgtctctat atcgccgctt gcgaccgcgg gcattatctc gtcgaaaggc
     3301 atttcgacgg ggataaaacc gtccgcgtat atactcagca gcaggaaagc agtagtgagc
     3361 tttcccggga cgcctatctc cttcccgcgg aggtcttctg tcggcacgca cgatactact
     3421 attgggccgt atccttctcc catgctcgcg ccgcaggaca tgatcctgta atcgtcctta
     3481 actttgagat acccgtgcgc ggaaatggct gtggtttcaa gctctttatt cagagcgcgc
     3541 ttgttaag
//
LOCUS       DASSJH010000326         4858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_403963, whole genome shotgun sequence.
ACCESSION   DASSJH010000326 DASSJH010000000
VERSION     DASSJH010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403963"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..970
                     /locus_tag="VFJ67_07110"
     CDS             71..970
                     /locus_tag="VFJ67_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7290194.1"
                     /translation="MIEIKNVTKYFGKLKAVDSVSFRAAKGEAVSLLGSNGAGKSTLI
                     KCILGLLDYTGDITVAGLRVREHPKEAKSLIGYLPQEPFFYDMRALDIVRFFARLRKI
                     DERKSMASLEKVGLAEHASKYATELSGGMRQRLSFAIALLSEPAFLILDEPTSNLDAH
                     ARAEFLRLIKEYKENGKTVIFSSHRLDEVDYLADRAVLMKDGKVVMDEEPHRLRQSLG
                     LGTKMNIAVPEEHASLASSAFSAAGIIVHGKNGRGFLVEVRGGDSLRPLRELMSRDIP
                     VLGFSVDEPTMENILEGAERNGN"
     gene            960..1799
                     /locus_tag="VFJ67_07115"
     CDS             960..1799
                     /locus_tag="VFJ67_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7290195.1"
                     /translation="MEIRNILILAGKETRESLRNRWFVLYTVSFSVLAVLILAIAPGR
                     SEVGGVSAYGRTAASLINLVLLFVPLIALVTGAISVSGERENGTLQYLLSHPVTKPEV
                     FVGKFFGILIPIFFSITLGFGLAGTGVALRGGGGDAAELVLTALLSGLLAASLLSVGF
                     VVSVFSGRASKAIGVAVFLWLFFIILGDLGIMGTAAAMDLGIKQVFALALLNPAEVFK
                     IASVLVLSPRFEILGPVGVYAVRTFGREGVIFLLLSVMALWTLVPFVYAFITFSVLRR
                     EET"
     gene            1796..2230
                     /locus_tag="VFJ67_07120"
     CDS             1796..2230
                     /locus_tag="VFJ67_07120"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HET7290196.1"
                     /translation="MKILLILLVVLSFTACRDNIDTGPHVIHYGEDVCERCKMIISDK
                     RFAAELVDQKGEALMFDDVGCMADYMKGSEKEGAKPLAMYVTDFSTGEWIDAGKAFYL
                     LNPELKSPMGYNIAAFGSSEAMKGSPFREGGKEAGGFGDLVK"
     gene            2327..2920
                     /locus_tag="VFJ67_07125"
     CDS             2327..2920
                     /locus_tag="VFJ67_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7290197.1"
                     /translation="MNQVNLKAADPGEKIQSAALKLFIKKGITGTTTKEIAKKAGVSE
                     GTIYNYFESKGDLAYKLFVRHMDRFRDELTEKTSSAAGPAEKLTTAVRAFFGFAKSNP
                     DAYAYIMIGHYTELQKMPYDKKKPRDIFVDIIGEGIGEGVFTDMDRNLGAAFVIGMVT
                     RAILFHTSGMLEIPYRQLADETVDSSLRVLGYRNSRL"
     gene            3066..3458
                     /locus_tag="VFJ67_07130"
     CDS             3066..3458
                     /locus_tag="VFJ67_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7290198.1"
                     /translation="MLVKECMTKDPVTCSPDEDVRVVFGRLTDMKIRQAPVLEGGKLI
                     GIVTDRDLRMAVVETVSGQGLTVRSVMTPKPVTVAEDSQLREAAGLLSKNKFNALPVL
                     SAAGELAGVLTTTDVLNGLVRALDAEKK"
     gene            3513..3944
                     /locus_tag="VFJ67_07135"
     CDS             3513..3944
                     /locus_tag="VFJ67_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7290199.1"
                     /translation="MKIEKILFPTDFSPRSRSAREHTLYLAGALGAEVYILHAIEPLK
                     YEEVDEEIKEFYRTLETQMSEKMSGEKEIFEKNGLGVNTDIIIGPRWKVINAYAEEKG
                     IDLIIMGSHGIRTDTGEVSVGTTSHKVMFSSPCPVLIVRHD"
     gene            4091..4837
                     /locus_tag="VFJ67_07140"
     CDS             4091..4837
                     /locus_tag="VFJ67_07140"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HET7290200.1"
                     /translation="MFIPRPGAAPFAQDESAQSVPQTPVIDPVADKLLKSMSATLSSA
                     KTLGFKADINFDQLIVSGQQIQYGGAAQVTLKRPDKVYAEYAGDLNAKKVWYTGGEVT
                     VLDLNTNFYGKLPVPSTLDEAMDTLMTQYGFTLPLSDVLVSDPYKAFVSHVAAGVVIG
                     DSTINGQVCKHLAFVEKYIDWQIWISEGAQALPCKLVITYKTLPGGPQYEAEFYDWAI
                     NPTITASAFTPVLPKGAEQIEFIKMKANNQ"
BASE COUNT         1142 a         1281 c         1460 g          975 t
ORIGIN      
        1 tccctgccca tggtcgcggt cccgctcgtt ttttacgctt atgtcatgaa acgcgggacg
       61 ggagtctgaa ttgatagaga taaaaaacgt tacgaaatat ttcgggaagc tgaaggcggt
      121 cgattccgtg agcttccgcg cagctaaggg agaagcggtg tctctcctcg gatcgaacgg
      181 cgcgggaaag agcacgctca taaagtgcat tctcggactc ctcgattata cgggagacat
      241 cacggtcgcc ggtctcaggg tgagggagca tccaaaggag gccaagtccc tcatcgggta
      301 cctgcctcag gagccgttct tctacgatat gcgcgcgctc gacatcgtgc ggttcttcgc
      361 gcgtctcagg aagatagacg agaggaaatc gatggcttca ctcgaaaaag tcggcctcgc
      421 tgagcacgca tccaaatacg caaccgagct ttcgggggga atgaggcagc gtctctcttt
      481 cgcgatagcg ctcctctcgg agcccgcgtt ccttatactg gacgagccca cctcgaacct
      541 cgacgcgcac gcgcgggccg aattcctgcg tctcataaag gaatataaag aaaacgggaa
      601 gacggtgatc ttttcctcgc acaggctcga cgaagtggat tatctcgccg acagggcggt
      661 cctcatgaag gatggcaagg tcgtcatgga cgaggagcct cacaggctga ggcagtccct
      721 cggtctcggc acgaagatga atatcgcggt ccccgaggag cacgcctcgc tcgcgtcttc
      781 cgctttctca gctgcgggca taatcgttca cggaaagaac ggcagggggt tcctggtcga
      841 ggtgaggggc ggggacagcc tccgtccgct gagagagctc atgtcgaggg atatacccgt
      901 cctcgggttc agcgtcgatg agcccacaat ggagaacata ctcgaaggag cggagaggaa
      961 tggaaattag gaatattctc atactcgcgg ggaaggaaac gagggagtca ctcaggaaca
     1021 ggtggttcgt cctttacacc gtatcttttt ccgttctcgc ggtgctgata ctcgcgatcg
     1081 ctccggggag gagcgaggtc ggaggcgtct cggcctacgg caggaccgcg gcgagcctga
     1141 tcaatctcgt cctcctcttc gtaccgctca tcgcgctcgt gacaggggcg ataagcgtat
     1201 cgggggaaag ggagaacggg acgctccagt acctcctctc gcaccccgtg acgaagccgg
     1261 aggtgttcgt cgggaaattc ttcgggatac tcatacccat cttcttctct ataacgctcg
     1321 gcttcggtct cgcgggaaca ggagtcgcgc taaggggcgg aggaggggac gcggccgagc
     1381 tcgtgctcac ggcgctcctc tcgggtctcc tggcggcatc gctgctgagc gtggggttcg
     1441 tcgtctccgt attttcgggc agggcgtcca aggccatagg ggtcgccgta ttcctctggc
     1501 tcttttttat aattctcggc gacctcggga taatgggcac tgcggccgca atggacctcg
     1561 gcataaaaca ggtattcgcg ctcgcgctcc tcaatccggc agaggtgttc aagatagcct
     1621 cggtcctcgt gctgagcccg aggttcgaga tactgggccc tgtgggcgtc tacgctgtga
     1681 ggacgttcgg aagggaaggg gtgatattcc tgctgctttc cgtcatggcc ctctggacgt
     1741 tagtgccgtt cgtatatgcg tttataactt tctccgtatt gaggagagaa gaaacatgaa
     1801 aattttactg atactgctcg tagtcctctc cttcaccgcg tgcagggaca atatagatac
     1861 cgggccccac gtcatacatt acggcgagga cgtctgcgag aggtgcaaga tgataataag
     1921 cgacaagcgc ttcgcggcgg agcttgtcga tcaaaaaggc gaggccttaa tgttcgacga
     1981 cgtcggatgc atggccgatt acatgaaagg ttcggagaaa gagggggcga agcccctagc
     2041 catgtacgtc acggatttca gcacagggga gtggatagat gcgggtaagg ccttctatct
     2101 cctgaacccg gagctcaagt cccccatggg ttacaatatc gccgccttcg gcagctcgga
     2161 ggcgatgaaa gggagcccgt tccgggaggg cggcaaggaa gcgggcgggt tcggagatct
     2221 ggtgaaataa attccgccgc gcgttcagct tcgtatcgga cgggagagat aaattgattt
     2281 cgggaatttt acaaatatca tataggatct cgtttaagaa tccgtcatga accaagttaa
     2341 tttgaaagcg gccgatcccg gcgagaagat acagagcgcc gcgctcaagc tcttcataaa
     2401 gaaaggcata acgggcacga caacgaagga aatagcgaag aaggcgggcg tctccgaagg
     2461 cacgatctat aactacttcg agagcaaggg cgacctcgct tataagctct tcgtccggca
     2521 catggacagg ttcagggatg agctaactga aaaaacctcg tccgccgcgg gtcccgcgga
     2581 aaaactgacg acggccgtcc gtgcgttctt cggtttcgcc aagagcaacc cggacgcata
     2641 cgcctacatc atgataggcc actacaccga gcttcagaaa atgccctacg acaagaagaa
     2701 gccgagggac attttcgtcg atataatagg cgaaggcatt ggcgaggggg tcttcaccga
     2761 catggaccgc aacctgggcg ccgctttcgt cataggcatg gtcacgcggg ccatactctt
     2821 tcatacgagc ggaatgctcg aaatcccgta ccggcagctc gccgacgaga ccgtggactc
     2881 gtctctgaga gtgctgggtt atcgtaacag ccggctgtaa taacgtacgg gaacattatc
     2941 agacattaaa tttaattacc ggatttgcgg gacggagtgc gcagattata gttatatcgc
     3001 gagcccgaca acggtgtccc gccccggctc ggccgggaag acgtaaataa agggaggaag
     3061 aaacgatgct cgtcaaggaa tgcatgacca aagaccccgt cacgtgctcg ccggacgagg
     3121 acgtaagagt cgtattcggc aggctcacgg atatgaagat acgtcaggcg ccggtgctcg
     3181 agggcgggaa gctcatcggg atagtgaccg acagggacct caggatggcc gtggtggaga
     3241 ccgtatccgg gcagggtttg accgtgcgct cggtgatgac tcccaaaccc gtaacggtgg
     3301 ccgaggactc gcagctgagg gaagccgccg ggctcctgag caaaaacaag ttcaacgctc
     3361 tgccggtgct gtcggccgcg ggggagctcg ctggcgtgct cacgacaacg gacgtgctta
     3421 acgggctcgt cagggcgctc gacgccgaga aaaagtgaca cttatatttc aaaatttttg
     3481 ggattagaat ataagccgca aggagacagg cattgaagat cgagaagata ttgttcccaa
     3541 cggatttttc acccaggtcc aggagcgcga gggagcacac gctctacctc gcgggtgcgc
     3601 tcggcgcgga ggtctatatc ctccacgcga tagagccgct caaatacgag gaggtcgacg
     3661 aagagataaa ggaattttac aggacgctcg agacccagat gagcgagaag atgtcgggag
     3721 agaaggagat tttcgagaaa aacgggctcg gcgtgaatac cgacatcatt atcggcccca
     3781 ggtggaaggt aataaacgcg tacgcggagg aaaaagggat agaccttatt ataatgggct
     3841 cccacggcat aaggaccgat acgggcgagg tctcggtcgg cacgaccagc cacaaggtca
     3901 tgttctcttc cccctgcccg gtgctcatcg taaggcacga ctgacccgtt taaattccat
     3961 taacgggatt tacataaaag agatataata ttagggcata atattaatgg aggagatgtc
     4021 agatatgacg aaatctttgc acaattacgt gcggttccta ctttttatag cccttgctgc
     4081 ggcgccgtta atgtttatcc cgcggccagg cgcggctcca ttcgcgcagg acgagagcgc
     4141 ccagtccgtg ccccagacgc ctgtcatcga ccctgtagcc gataagctgc tgaagtcgat
     4201 gagcgccacg ctgtcctcgg ccaagacgct cggcttcaaa gcggatatca acttcgacca
     4261 gctaatcgtt tccgggcagc agatacaata cggaggcgcg gctcaggtga ctctcaagag
     4321 gcctgacaag gtgtacgccg agtacgccgg ggacctcaac gcgaagaagg tctggtacac
     4381 gggcggcgaa gtcacggtgc tcgacctgaa cacgaatttt tacggcaagc tccccgtccc
     4441 ctcgactctc gacgaagcca tggacaccct gatgacgcaa tacggattca ctctcccgct
     4501 ttcggacgtg ctcgtgagcg acccgtacaa ggcgttcgtc tcgcacgttg cggcgggcgt
     4561 cgtgatagga gacagcacga tcaacgggca ggtgtgcaag catctggctt tcgtcgagaa
     4621 gtatatcgac tggcagatct ggatctcgga gggcgcgcag gcgcttccct gcaagcttgt
     4681 aataacgtac aaaaccttac cgggcggacc tcagtacgag gctgagttct acgactgggc
     4741 tatcaatccg actatcaccg cgtcggcatt cacgcccgtt ctgcccaagg gcgcggagca
     4801 gatcgagttt atcaagatga aggctaataa ccaataacga cagggggacc gaaatgag
//
LOCUS       DASSJH010000327         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1377121, whole genome shotgun sequence.
ACCESSION   DASSJH010000327 DASSJH010000000
VERSION     DASSJH010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377121"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFJ67_07145"
     CDS             complement(<1..416)
                     /locus_tag="VFJ67_07145"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7290201.1"
                     /translation="MSESKLVVTKVIKATPEEVFEAFTDPEIMRKWFYGDEGWTVDVS
                     NTLEIGGKYILNMIKPDGKEYKHTGEYKVIAPPEKLVFTWNSDFAQDTVVTVYFRGVA
                     GGTEITLEHEFLPTDEAREDHRRGWTVCLGNLARLYA"
     gene            complement(413..763)
                     /locus_tag="VFJ67_07150"
     CDS             complement(413..763)
                     /locus_tag="VFJ67_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7290202.1"
                     /translation="MVDYQNTDLDSILHALANSTRRGIIMQLAKKDLTVNELADKYDM
                     SLQAVSKHIQVLVKSGLVVQEKEGRIKHCRANIEPLDRVSDLIRQYGRFWGERLDALD
                     KYFEGRKKTKERAK"
     gene            977..1813
                     /locus_tag="VFJ67_07155"
     CDS             977..1813
                     /locus_tag="VFJ67_07155"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HET7290203.1"
                     /translation="MARYTYFMRLIALIFIVSGTCIRGSAAAESAITESIGSYTAGCI
                     RNAAALPASGEGFQVIRLDRGRYYANPEMIEYIESLAKTVSEQLGAILLIGDVAQRTG
                     GPMYDEHSSHQIGLDADILFWQHPIALRRQLTVMERAHIYPQSVLNEEQTAIDDFKWD
                     VKIEEIIKIAASDKRVDRIFVNPIIKRRLCQTHPGEKWLAKVRPWYGHDGHFHVRLKC
                     PPGNMLCETQEPLNMFDDGCGPELDGWFRADGRVKSQPRSGKASHAKLPPACIPILNG
                     SY"
     gene            complement(1882..2244)
                     /locus_tag="VFJ67_07160"
     CDS             complement(1882..2244)
                     /locus_tag="VFJ67_07160"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET7290204.1"
                     /translation="MVQVIFISYVLLVILLSLFPQPAGTGGGYLDKIIHASLYCVMGV
                     LAYIAFNTRGKRIVMFLFMLLLGISLELLQTYIPGRDASVYDALANTAGLALSFFLCW
                     LYTIIPDTQPPGAGDISR"
BASE COUNT          714 a          734 c          663 g          637 t
ORIGIN      
        1 gcatataatc tcgcgagatt ccctaggcat accgtccacc ctctcctgtg gtcctcgcgc
       61 gcctcgtccg tcggtaagaa ttcatgctcg agagtgatct ctgtcccacc cgcgacaccg
      121 cggaaataaa cggtaacgac cgtatcctgc gcgaaatccg agttccaggt gaagaccagc
      181 ttctcaggcg gcgcgataac tttatactcg cctgtatgtt tatattcttt accatcgggc
      241 ttaatcatat ttaagatata tttccctccg atctccaatg tattggaaac atcgactgtc
      301 catccctcgt ccccgtaaaa ccattttctc atgatctcgg ggtcggtgaa cgcttcgaac
      361 acctcttcag gcgtcgcttt tatgaccttt gtcacaacca gcttgctttc gctcatttcg
      421 cacgctcctt tgtctttttg cgtccctcaa aatatttatc aagagcatcg agcctctctc
      481 cccagaaacg gccgtactgc cttatcaagt ccgacacgcg gtccagcggc tctatattgg
      541 cgcggcagtg ttttatgcgg ccttcctttt cctggacgac gagccccgat ttaacgagca
      601 cctgtatgtg cttggagacg gcctgaagcg acatatcgta tttatcggcg agctcgttaa
      661 cggtcaggtc ctttttggcg agctgcataa tgattcccct gcgcgtggag ttggccagag
      721 cgtgcagtat cgaatccagg tccgtattct gataatcaac catatagttg attatatcag
      781 ggctccccgt aattgtcaac catatagttg aatatttatc cggcgagaca aaaattcgag
      841 aatatataac cgccgttgtc catacccttt atgactccgc cggttcatgt ataattccct
      901 tcatatatcg tgtctcgata tatatggcgc cgtgacggca gtatccgacc ggacccacgc
      961 accccgtaca atcaatatgg caagatatac gtactttatg cgcctgatag cacttatctt
     1021 tatagtcagc ggaacgtgca tccgcggctc ggctgccgcg gagtccgcga tcacggaatc
     1081 gatcggctcg tacaccgccg gctgcatcag gaacgccgcc gcgctgcctg cgagcgggga
     1141 agggttccag gtcataaggc tcgacagggg cagatactac gccaatccgg aaatgatcga
     1201 atacatcgaa tccctcgcaa agaccgtcag cgaacagctg ggagccattc tcctcatcgg
     1261 ggacgtcgcg caaaggacag gggggcccat gtacgacgaa cacagcagtc atcagatcgg
     1321 cctcgacgca gatatacttt tctggcagca ccctatcgcg ctaaggagac agctcaccgt
     1381 catggagagg gcgcacatat acccgcaatc agtcctcaac gaggagcaga cggctataga
     1441 cgacttcaaa tgggacgtaa agatcgaaga gattatcaaa atcgccgcct ccgacaagag
     1501 ggtggaccgg atattcgtga acccgatcat caagaggagg ctctgtcaga cacatccggg
     1561 cgagaagtgg ctcgcaaaag tgaggccgtg gtacgggcac gacggacatt tccacgtccg
     1621 tctcaagtgt cctccgggca acatgctctg cgaaacccag gagccgctca acatgttcga
     1681 cgacggctgc ggtccggaac tcgacggatg gttcagagcg gacgggagag taaaatcgca
     1741 gccgagatcg ggtaaagcct cccatgcgaa actccctcca gcctgtatcc ctattctgaa
     1801 cggctcgtat tgaaacccgg gcctgttaaa ccttccggca gcgctagtcg ggcgcttccc
     1861 ggatccgcaa tgcacctaaa tttaccggct tatatcgccc gctccgggcg gttgtgtatc
     1921 gggaattatc gtatacagcc agcagaggaa aaagctgagg gcgagcccag cagtgtttgc
     1981 tagggcatcg tagacggatg catcccttcc cggaatgtac gtttgaagca gctccagcga
     2041 tatgcccaga agcagcatga ataaaaacat aacaatcctt ttgccgcgcg tattgaaggc
     2101 tatataggca aggactccca ttacgcagta gagggaagcg tggataatct tatccagata
     2161 gccgcctccg gtcccggcgg gctgcggaaa gaggctgagc aatatcacga gcagcacata
     2221 agatatgaat atgacctgga ccatggatcg acgatcagcg atagttagag gctctattat
     2281 acacgatgcg gagggatgga aaattctgtc atcgacggcc ggaaatccaa aaaatcataa
     2341 tgcggcaggg aaactccgga tgtgatacga aaacccgccc cgactgattt tccgaattct
     2401 ttcgcaagtc cggaaagccg tttatgcatc tttctgtgcg tagtatttat cgagtggctt
     2461 caattggctg tgaaatctat ttgcaaatat ccattatata attcacacgc agctaaaata
     2521 aaccattaaa cagttcattc aatttaagca gttacttgag gatcagatta gaaacggctg
     2581 ttgtaacgta atacacgctc cacaccgatg agggaaatac aaagaattgc tggtaattcg
     2641 agacagattg aatgaggcta accctccgta atagctacaa ttaatcccac tcttaaagaa
     2701 acacttgaag aataaccaac aacttgctat aattaatacg aaaccaga
//
LOCUS       DASSJH010000328         9251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_613661, whole genome shotgun sequence.
ACCESSION   DASSJH010000328 DASSJH010000000
VERSION     DASSJH010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9251
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613661"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFJ67_07165"
     CDS             <1..319
                     /locus_tag="VFJ67_07165"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET7290205.1"
                     /translation="VKTDAGDFIGRDALCGVLAEGPGHRIYGFEMIDRGIPREGYRVF
                     KDGTHIGRVTSGTLSPSLEKSIGMGYLNSGVVLGDSIQIEIRNTFREARVVNIPFYKK
                     DVN"
     gene            332..727
                     /gene="gcvH"
                     /locus_tag="VFJ67_07170"
     CDS             332..727
                     /gene="gcvH"
                     /locus_tag="VFJ67_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET7290206.1"
                     /translation="MVQIPDELKYTGDHEWAMEEGDLLVIGITDYAQDALGEIVYIEL
                     PSEGDEITKGDPFGAVESTKAVSDLYAPVSGDVVEVNDVLLDTPETINGDPYGEGWMI
                     KVRPYDPKELNDLMDFEEYAEYVEKEMEE"
     gene            843..3746
                     /gene="gcvP"
                     /locus_tag="VFJ67_07175"
     CDS             843..3746
                     /gene="gcvP"
                     /locus_tag="VFJ67_07175"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HET7290207.1"
                     /translation="MKTEKDGYRTFARRHIGPGRTELAQMLEAVGAQSLDQLVEETVP
                     ASIRLKKQLNLPEGISEYALLEDLRKKASMNRVYKSFIGLGYYDCVTPGVIQRNILEN
                     PGWYTQYTPYQAEISQGRLEALLNFQTMVVDLTGMDIANASLLDEGTAAAEAMTMLHR
                     VAEKKQGGGDAAVFFVSEKCFPQTIDVLTTRAEPLGIKLVVGDHTAFEFGGGVFGALV
                     QYPDTDGEVCDYSDFINRAHSNGALVAVAADLLSLTLLTPPGEFGADAVLGLAQRFGV
                     PLGFGGPHAAYFATRGAYTRQVPGRIIGVSIDSHGDPAYRMALQTREQHIRREKATSN
                     ICTAQALLAIMASMYAVYHGPTGLKAIAGRIHRSAAVLESALTGLGMTQKNRHYFDTL
                     KIDLGEGSDVLISKIRAGAERSRINFNYIDGRHIGISLDETTSPDDVREIVKVFSNAL
                     VNESKAPDINISAWDSDPRIPSGLARKSPYLAHPVFNSYHSETLMLRYIKRLESKDLS
                     LTTSMIPLGSCTMKLNATAEMVPVTWPEFSRLHPFAPPDQTRGYLRIFRELERYLSVI
                     TGFPAVSLQPNSGAQGEYTGLMVIRAYYRDRGEGRRNVALIPSSAHGTNPASAALAGM
                     KVVVVDCDERGNIDLASLRKKAGEHKDELAALMVTYPSTHGVFEEKIKEICSIVHRNG
                     GQVYMDGANMNAQVGLTNPAIIGADICHLNLHKTFSIPHGGGGPGMGPICARKHLAPY
                     LPGHPLVKTGGEKPIRAVSSAPWGSASILLVSYAYIRLLGRDGVADATRYAILNANYI
                     KSRLEKIYDVLYTGSRGRVAHEFILDMRRFKKDAGIEVEDVAKRLMDYGFHAPTISWP
                     VAGTMMIEPTESESKAELDRLCDALIAINEEVKEISEGISDREDNVLRNAPLTAMEAA
                     GDSWIHHYSRHKAAYPLPYLEEHKFWPPVGRINNPYGDRNLVCTCPDIKSYAE"
     gene            complement(3786..4673)
                     /locus_tag="VFJ67_07180"
     CDS             complement(3786..4673)
                     /locus_tag="VFJ67_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HET7290208.1"
                     /translation="MSVRPGLQNINFLYPILIIVLIILATSSFVIVESGHVGVVRTLG
                     AVQPTPLTEGFHFKKPFIDKIEQVDVRLTKAQSKSAAASKDLQTVETQVTVQYSLMGP
                     TAPETFQKIGNRDVVAATVIEPAIQESVKAITAQYTAEQLVTKRAEVKLQIQEAIDNF
                     IDVTLKDKSVPAALKISNVAITDFEFSAEFNRAIELKVKAEQEALQARNEKTKRVTQA
                     EAAYQEQKLAADAAAYDIEARSKAKADAIAREAKALKDNPELIQLRLSEKWDGTLPKF
                     TGGEAIPFIDINSVINDKQ"
     gene            complement(4761..6455)
                     /gene="recJ"
                     /locus_tag="VFJ67_07185"
     CDS             complement(4761..6455)
                     /gene="recJ"
                     /locus_tag="VFJ67_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET7290209.1"
                     /translation="MKKNWAIRKENRKLTDKISKSLGISPLTAQILVNRGIGNETEAN
                     LFLNSTLFDLPSPYLMKGMDRAVERIKRAIVNREKIAIYGDYDVDGVTSTALFYTFLK
                     GLGADVVFYNPDRLREGYGVNIEAVRKLAEGGVKLFISGDCGITAVKEVEEAKRLGAD
                     FIVTDHHKPPEELPDAVSILNPQLPDCKYPGKTIAGVGVMFNFAVALRRALREDGFFR
                     NGEPNLADYLDLVALGTVADCAPLTDVNRIFVREGMKRMENPKRTGILALKEASSITG
                     AVSTFDLGFKLGPRINASGRLSTAGKAVELFISDDLSDAREIAKALSRENSNRQSIEG
                     EILSDAVSQIESVPGILNPNSIVIASPDWHPGIIGIVASRIVERYERPAILIAIDRNG
                     IGKGSGRSVEGVNIYSALSECRELFEQFGGHEQAAGLSIRAEKIERFREMFEKSIAGS
                     GHSYEPRLDIDYEVEFNQLTDALLTELGMLEPYGIGNPEPLLAARSVEVVSQRTFKDK
                     HLGLRLRQGNVSLDGIWFNSGEISTLPDKIDIVFTPEFNKWNGGKDIRLRIIDGSW"
     gene            complement(6558..7001)
                     /gene="aroQ"
                     /locus_tag="VFJ67_07190"
     CDS             complement(6558..7001)
                     /gene="aroQ"
                     /locus_tag="VFJ67_07190"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET7290210.1"
                     /translation="MKILVIHGPNLNMLGKREPSIYGASTLREIDRLIREEAKKLDVK
                     TEIFQSNSEGEIVSRIQDAMKKSDGILINPGAYTHTSIAIRDAILSAGLPVVEVHISN
                     IYKREDFRQKSYVSGVAVGVISGFGTDSYILGLGALVSHLRARAE"
     gene            complement(7010..7735)
                     /locus_tag="VFJ67_07195"
     CDS             complement(7010..7735)
                     /locus_tag="VFJ67_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7290211.1"
                     /translation="MTGDNHSTEDELVKFEEKYARILSQDPSSVTFIFLAQVLHKQGK
                     VDKAIKVLINGLKYNRNSVTGRFLLGKIYYDRWLIEQAKREFKAVIELAPDNLAACKM
                     LVQIHKSEEAYGRAHELLKTALAYHPHDASIMTEMTEIEALLSSAEKKENEFRSAREN
                     IRTALESKSAEELESGLKNQEKELFTETIADIYIAQGLYEKACGVLEKMLEKDPGNDA
                     VSKKLARSRLYLINKSAGFIRGE"
     gene            8090..>9251
                     /locus_tag="VFJ67_07200"
     CDS             8090..>9251
                     /locus_tag="VFJ67_07200"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HET7290212.1"
                     /translation="MLRRLKVVFSLLLLLSLPAWGYNTDNGGIYDDNGERVHLYGVSW
                     FGFETGDHVVHGLWARNWKDMITQIKGLGFTAVRVPFCPATLDNTSVSSINYALNPDL
                     QGLKSLDILDRVVNELDSRGIYIVFDFHNYDCQSINELWYSGSYSEQNWIDDLVFITK
                     RYMNVAHVVGIDIKNEPHGQATWGTGSVSTDWNTAADKAADAVLAADPGLLVFVQGIQ
                     DNPVCSGTLSHWWGGNLEPFECYPLDVPADKVVLSPHVYGPDVFVQPYFNASNFPDNM
                     PDIWETHFGYLTDLGYSVVIGEFGGKYGHGGDSRDKTLQDALISYMDSKGMTDFFYWS
                     WNSNSGDTGGILQDDWTSVWQDKVDLLRRLMDGAGGGTGDDPACSDGLDNDGD"
BASE COUNT         1955 a         2652 c         2536 g         2108 t
ORIGIN      
        1 tgtgaaaacg gacgccgggg actttatagg cagagacgcc ttgtgcggag tcctcgcaga
       61 gggtccaggg caccggatat acgggttcga gatgatagac cgcggcatac cgagagaggg
      121 ttacagggta tttaaggacg gcacgcacat aggcagggtc acgagcggga ctttatcccc
      181 gagccttgaa aaatcgatcg ggatgggtta tttaaacagt ggcgtcgtat tgggcgacag
      241 catacagatt gaaataagaa atactttcag ggaggctcgg gtagtaaata tccccttcta
      301 taaaaaggat gttaattaag gagatctgac gatggtacaa attcctgacg aactcaaata
      361 caccggcgat cacgaatggg cgatggaaga aggagatttg ctcgtgatcg gtattacgga
      421 ttacgcgcag gacgctttag gcgagatcgt ctatatcgag ctaccgagcg aaggggacga
      481 aataaccaag ggcgaccctt tcggcgctgt tgaatcgaca aaggccgtgt cggatctgta
      541 cgcccctgtg agcggcgacg tggtcgaggt caacgacgtg ctgctcgaca cacccgagac
      601 catcaacgga gacccgtacg gggaagggtg gatgataaag gtgaggcctt atgaccccaa
      661 agagcttaac gatttgatgg acttcgaaga atacgcggag tatgtagaga aggaaatgga
      721 agaataagat taaatgacag attctttccg tgcttcgcgt ccatgtgcgg gcgcgaatct
      781 attcggtcgg acaatttttc ctccggttat aattatcaaa tcccggtaat gctaacagta
      841 agatgaaaac tgagaaggac ggataccgta cattcgcgag gaggcacata ggcccgggcc
      901 ggacggagct cgcccaaatg ctcgaagcgg tcggcgcaca gtccctcgat cagctcgtcg
      961 aagaaacggt ccccgcttcc ataagactga aaaaacagct caatctcccc gaaggcataa
     1021 gcgaatacgc gctgcttgag gatttgagaa agaaagcgtc catgaaccgg gtctataagt
     1081 cctttatagg cctcggttat tacgactgcg tcacgcccgg cgtcatacag aggaacattc
     1141 tcgagaaccc gggctggtac acccagtaca ccccctatca ggcggagata tcgcaggggc
     1201 gtctcgaagc gctccttaat ttccagacca tggtcgtcga cctcaccggt atggatatag
     1261 ctaatgcgtc ccttctcgac gaaggcactg cggctgctga agcaatgacg atgctccacc
     1321 gtgtagcgga gaagaagcag ggcggcggcg atgcggccgt cttcttcgtt tcggagaagt
     1381 gcttcccgca gacgatcgat gtcctcacga cgcgggcgga gccgctcgga ataaagctcg
     1441 tcgtcggaga ccacacggct ttcgagttcg gcggcggcgt attcggcgcg ctcgttcagt
     1501 atcccgacac ggacggcgag gtatgcgact attcggattt cataaacagg gcgcactcga
     1561 acggcgctct cgtcgcggtc gcggccgacc tgctctctct cacgctcctt acgccgccgg
     1621 gagagttcgg tgccgacgct gtcctcggct tggcgcagag gttcggggtc ccgctcgggt
     1681 tcggagggcc gcacgcggcc tacttcgcca cgcgcggggc atatacgcgc caggtgcctg
     1741 ggaggataat cggggtatct atagactctc acggggaccc cgcctacagg atggcccttc
     1801 agacgagaga gcagcacata aggagagaga aagcgacctc caacatctgc accgctcagg
     1861 cgctcctcgc tatcatggcc tcgatgtacg ccgtctatca cggccccacg ggcctgaaag
     1921 ccatagccgg gaggatacac aggtcggcgg cggttctcga aagcgcactc acggggctcg
     1981 gaatgactca gaagaaccgg cattacttcg acacgcttaa gatagacctc ggagaaggct
     2041 cggacgttct tatatccaaa atcagggccg gcgctgaaag gtcccgcatc aatttcaatt
     2101 acatcgacgg ccgccatatc gggatatccc tcgacgaaac tacgagtccc gatgacgtcc
     2161 gggaaatcgt gaaagtcttt tccaacgcac tcgttaatga atcaaaggct cctgatatca
     2221 atattagcgc ctgggattcg gatccgagga taccgtccgg gctcgcgcga aagagtccct
     2281 acctcgcaca cccggtcttc aacagctacc actcggaaac gctgatgctg agatatataa
     2341 agcggctcga gagcaaggac ctctcgctca cgacatcgat gatacccctc ggctcctgca
     2401 cgatgaagct gaacgccact gccgaaatgg tccccgtcac gtggcccgag ttctcgaggc
     2461 tccatccgtt cgccccgccc gatcagacac gcggctacct gcgcatattc agggagctcg
     2521 agcgttatct ctcggtgatc acggggttcc ccgccgtttc gctccagccc aattcgggcg
     2581 cacagggcga gtatacgggg ctcatggtca taagggccta ttacagggac aggggcgagg
     2641 ggcggaggaa cgtcgccctc ataccgtctt cggcgcacgg cacgaacccc gcgagcgcgg
     2701 cgcttgccgg catgaaggtc gtcgtcgtcg attgcgacga gcggggaaat atagacctcg
     2761 cctcgctcag gaagaaggcc ggtgagcaca aggacgagct cgcggcgctc atggtcactt
     2821 acccctcgac ccacggcgtc ttcgaggaga agataaagga gatttgctcg atcgtgcaca
     2881 ggaacggagg ccaggtctac atggacgggg ccaatatgaa cgcgcaggtg gggctcacga
     2941 acccggcgat aatcggggcc gatatatgcc acctcaacct gcacaagacg ttcagcatcc
     3001 cgcacggcgg cggaggcccg ggaatgggcc cgatatgcgc gaggaaacac ctggcgcctt
     3061 acctgccggg tcacccgctc gtaaagaccg ggggagaaaa accgatacgc gccgtatcgt
     3121 cggcgccctg gggtagcgcg agcattctgc tcgtctcata tgcgtatatt agattgttgg
     3181 gcagggacgg agttgcggat gcaaccaggt acgccatact caacgcaaat tatataaagt
     3241 cgaggcttga aaagatatac gacgtcctct acacgggctc gcgcggcaga gtggcccacg
     3301 agttcatact cgatatgagg cgttttaaaa aggacgccgg catcgaggtc gaggacgtcg
     3361 ccaagaggct gatggattac ggcttccacg cccccacgat ctcgtggcct gtcgccggga
     3421 ctatgatgat agagcccacg gagagcgaat ccaaagcaga gctcgaccgc ctctgcgacg
     3481 cccttatcgc gataaacgaa gaggtgaagg agatctcgga gggcatctcc gacagggagg
     3541 acaacgtgct caggaacgcc cctctcacgg cgatggaagc cgcgggggac agctggattc
     3601 accactattc gaggcacaag gccgcgtacc cgctccccta tctcgaggag cataaattct
     3661 ggccccccgt cggaaggata aataacccgt acggcgacag gaacctcgtc tgcacctgtc
     3721 ccgatatcaa gtcctacgcc gaataattcc cgcccggccc gttactccgt tcaggaaatt
     3781 caatctcact gcttgtcgtt aattaccgaa ttgatgtcta tgaacggtat agcctcgccc
     3841 cctgtgaact tcggcagagt gccgtcccat ttctccgaca gcctcagctg tatgagctcc
     3901 gggttgtcct tgagcgcctt cgcctcacgc gcgatcgcat cggctttggc cttcgaccgg
     3961 gcttcgatgt cgtacgcggc cgcgtcggct gcgagcttct gctcctgata cgcggcttca
     4021 gcctgcgtta ccctcttcgt tttttcgttc ctcgcctgaa gggcctcctg ctccgccttc
     4081 accttgagct cgatcgccct gttgaactca gccgagaact cgaaatctgt tatagcgacg
     4141 ttcgagatct taagggccgc cgggacgctt ttgtccttta gcgtcacgtc gatgaagttg
     4201 tctatggcct cttgtatctg gagcttgact tcggcgcgct tcgttacgag ctgctctgcc
     4261 gtgtactgag cggttatcgc ctttactgat tcctgtatcg cgggctcgat aaccgtggcc
     4321 gccacgacgt cgcggttgcc tattttctga aacgtctcgg gagccgtcgg acccatgagc
     4381 gagtactgca ccgtgacctg agtctccacg gtctgcaggt ctttggacgc ggcagcggac
     4441 ttggactgcg ccttcgtgag cctgacgtcg acctgctcga tcttgtctat gaacggcttc
     4501 ttgaagtgga agccttccgt aagcggcgtc ggctgcaccg ccccgagagt cctcacgacg
     4561 cccacgtgac cgctttccac tatgacgaac gagctcgttg cgaggatgat cagaacgatg
     4621 atcaatatcg ggtaaaggaa atttatattc tgtaatccgg gtcttactga cataacacac
     4681 ccccctttgc ttcttttttt acgagtatat taatatagcc ggaaaaggtt tataccgtga
     4741 gagggagatt tattggagtc ctaccagctt ccgtctatta ttctgagcct tatgtcttta
     4801 ccgccgttcc acttgttgaa ttccggcgtg aacacgatat caattttatc gggcagcgtg
     4861 gagatttctc ccgaattgaa ccatataccg tcgagggaga cgttcccctg cctgagcctg
     4921 agtcccaggt gtttgtcctt gaacgtgcgc tgcgatacga cttccaccga ccttgcggcc
     4981 agaaggggct cggggttccc tatcccgtat ggctcgagca tgccgagctc ggtgaggaga
     5041 gcgtccgtga gctgattaaa ttcaacttcg tagtctatgt cgagcctcgg ctcgtaagag
     5101 tggcccgagc ccgcgataga tttctcgaac atttccctga accgctcgat cttctcggcc
     5161 ctgatggaaa ggcccgcagc ctgctcgtgt cctccgaact gctcgaagag ctccctgcat
     5221 tcggatagcg cgctgtatat gttcacgccc tcgacgcttc tccccgaccc cttgccgatg
     5281 ccgtttctgt cgatcgctat cagtatcgcg ggtctctcgt agcgctcgac tatcctcgag
     5341 gccacgatgc ctattattcc cggatgccag tcgggcgacg cgataacgat cgaattcggg
     5401 ttaagtattc cgggcacgga ttctatctga ctcaccgcat ccgagagaat ttctccctcg
     5461 atgctctgcc tgttcgagtt ctcccggctg agcgctttcg ctatctctct cgcgtcactt
     5521 aaatcgtcgg atataaagag ctcgaccgcc ttcccggccg tgctaagcct tccggaggcg
     5581 tttatcctcg ggcccaattt gaacccgagg tcaaacgtgc ttacggcgcc ggttatgctg
     5641 ctcgcttcct tgagcgcgag tatgcccgtc ctcttcgggt tctccatcct cttcatgcct
     5701 tctctcacga atatgcggtt cacgtccgtg agcggcgcgc agtcggccac tgttccgagg
     5761 gccacgaggt cgaggtaatc ggctaggttc ggctccccgt tcctgaagaa gccgtcttcc
     5821 ctgagcgcgc gcctgagcgc gaccgcgaag ttgaacataa cccccacgcc cgcgatggtc
     5881 ttccccggat acttacagtc cgggagctgg gggttcagga tggacacggc gtccggcagc
     5941 tcctcgggcg gcttatggtg gtcggtcact ataaaatccg cgcccagcct cttcgcttcc
     6001 tccacttctt taacggccgt tatcccgcag tcgcctgaaa tgaagagctt cactcctccc
     6061 tcggccagct tcctcacggc ctctatgttg acgccgtacc cctccctcag cctgtcaggg
     6121 ttgtaaaata cgacgtccgc tccgagcccc ttgaggaacg tgtagaagag cgccgtggac
     6181 gtaaccccgt ccacgtcgta gtcgccgtat atcgcgatct tttcccggtt gactatcgcg
     6241 cgcttgatcc tctcgaccgc cctgtccatc cccttcatta gatagggcga cggaaggtcg
     6301 aagagcgtgc tgttgaggaa gaggttcgcc tcggtctcgt tcccgatccc cctgttgacg
     6361 agtatctgcg ccgtgagggg cgagatgccg aggctcttcg agattttgtc cgtcagcttc
     6421 cggttttctt ttcttatggc ccagttcttc ttcatgagag cgaaattata agatacataa
     6481 tgattacgat gcggggtgtt tttactgcgg cttcgtgccg gggagccgtt accgaaatta
     6541 tattgcggtt ctccgcctca ttccgccctg gctctcagat ggctcacgag tgcgccgagg
     6601 ccgagtatgt agctgtccgt gccgaacccc gatataacac cgaccgcaac ccccgagaca
     6661 tacgacttct gcctgaagtc ctccctcttg tatatattgg agatatggac ttccacaacc
     6721 ggcagacccg cagatagtat ggcgtccctt atggcgatgc tcgtgtgcgt gtacgctccg
     6781 gggttgatga gtatcccgtc cgattttttc atcgcgtcct ggatcctgct cactatctcc
     6841 ccctctgagt tcgactggaa tatttcggtc tttacgtcga gctttttcgc ctcctccctt
     6901 ataagcctgt ctatctccct gagcgtgctt gcgccgtata tcgaaggctc cctctttccg
     6961 agcatgttca ggttgggtcc gtgtatgacg agtatcttca tgtgtgcgtt cactctcctc
     7021 ttatgaaacc ggcgctttta ttaattagat agagcctaga ccgggcgagc ttcttcgaaa
     7081 cggcgtcgtt gccgggatct ttctccagca tcttttcgag cacgccgcac gccttttcgt
     7141 agagtccctg cgcgatataa atgtcggcta tagtctccgt aaaaagctcc ttctcctgat
     7201 ttttcaggcc cgactccagc tcttccgcgc tcttcgattc gagcgccgtc ctgatattct
     7261 ctctcgcgga ccggaattcg ttctcctttt tttccgctga agaaaggagg gcctcgatct
     7321 ccgtcatttc ggtcatgata gaagcgtcgt gcggatggta cgccagggcc gttttaagca
     7381 gctcgtgagc cctgccgtac gcttcctcgc tcttgtgtat ctggacgagc atcttgcatg
     7441 ccgccaggtt gtcgggagcg agctcgatca ccgccttgaa ttccctcttc gcctgctcta
     7501 tgagccacct gtcatagtat atcttcccca gcagaaacct gcccgtgacg ctgttcctgt
     7561 tatatttcaa cccgtttatc aaaactttaa tggccttatc caccttcccc tgcttgtgga
     7621 gcacctgggc gaggaagata aaagtgaccg acgaggggtc ctgcgagagg attcttgcgt
     7681 atttttcttc gaacttgacg agctcgtctt cggtcgagtg gttatcgccg gtcattgtcg
     7741 ttctgatatt acttcataca gcgggtcggg gtcaagaaag cccccgctcg gtgaattaaa
     7801 cgcgggcatg accgcctgtg ctgctccgtc ccgcattgct aaaatcataa cataatactt
     7861 gtaatagaaa cacagcgctc atccggtctc ggtgttacca tcgtatatat cgtgagaata
     7921 ttgtgctgcg aataccggat gtaggttgac ccgcatcggc gggagtccga tcaggtcagc
     7981 tttactctcg cttgtgcgca tccggtgtaa tcccgggacg tcgaattttt tcttcgccct
     8041 gacagattta taggttagaa ctgatataaa ccaacttgag ggtattctca tgcttcgacg
     8101 actgaaggta gttttttctt tgctactttt actttctctt cctgcctggg gttataacac
     8161 ggataacggt ggaatatacg acgacaatgg agaaagggtt cacctttacg gagtaagctg
     8221 gttcggcttc gagaccggtg accacgtagt ccacggactc tgggccagga actggaaaga
     8281 catgataacc cagataaagg ggctcgggtt tacggcagtc agggtcccct tctgtcccgc
     8341 gaccctcgac aatacatctg tgtccagcat caactatgcg ctcaaccccg acctccaggg
     8401 gctcaaatcc ctcgacatac tcgacagagt ggtaaacgag ctcgactccc gaggaatata
     8461 tatcgtcttc gattttcata actacgactg ccagtctata aacgagctct ggtactcggg
     8521 ctcctactca gagcagaact ggattgacga cctagtattc atcactaaga gatacatgaa
     8581 cgtcgcgcat gtcgtcggaa tagacataaa gaacgagccc cacggccagg ccacgtgggg
     8641 gacgggcagc gtatcgaccg actggaacac cgctgccgat aaggcggcgg acgccgtcct
     8701 cgcggccgat cccggacttc tggtattcgt ccagggcatc caggacaatc ccgtatgcag
     8761 cggtacgctc agccactggt ggggaggaaa cctcgagcct ttcgagtgct atcccctcga
     8821 cgtccccgcc gataaagtcg tccttagccc tcatgtttac ggcccggacg tattcgtaca
     8881 gccgtatttc aacgcctcca actttcctga caacatgccg gatatatggg aaacccattt
     8941 cggatacctc accgacctcg ggtactcggt ggtcataggc gagttcgggg ggaagtacgg
     9001 gcacggaggc gattcgagag acaagacgct acaggacgcg cttataagct acatggactc
     9061 gaaaggaatg accgatttct tctactggtc gtggaactct aacagcggcg acacgggcgg
     9121 aatattgcag gacgattgga cgagcgtctg gcaggacaag gtcgatctcc tgcgaaggct
     9181 tatggacgga gccggaggag gcacggggga tgaccccgca tgctcggacg gtctagacaa
     9241 cgacggggac g
//
LOCUS       DASSJH010000329         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1378228, whole genome shotgun sequence.
ACCESSION   DASSJH010000329 DASSJH010000000
VERSION     DASSJH010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378228"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1560
                     /locus_tag="VFJ67_07205"
     CDS             247..1560
                     /locus_tag="VFJ67_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7290213.1"
                     /translation="MPLPYPVIVIPGITATYLKNEYDLPPEYVWTVMTKKYERLALHP
                     NDLRYEVSEPARLKPDQLYEIAYKELIEELRYNLRSEEDKQVPVFPFGYDWRMKLEDI
                     QSELDSFVQEVIDRTKLLKHYYRAGYQDDPKVNLVGHSMGGLIIAGYLKSKGRQAPVH
                     KVVTFATPYQGSFEAVIKVTTGTANLGTSPPSSRERESARVTPALYYLVPSFSGGLEV
                     PPGVPQSLFDAGAWQPSIVQSIKEWIRLKGLPTPDQDALAEQIFSSMLKSGKNHRANI
                     DTFKLSDAGMTADRWLAVVGVDSVTRVRLKIVKNGRFPEFEFKTSDRDNKWFPARITD
                     PECRLTGDGTVPFEGAVPKFLAPENLVCVTPGDYGYWEIGDKVLTEFGGFHGIMPKMN
                     MLHRLLVRFFTDAPDKRDNTWGRRAPGVADGQWKPPLSLKDKTLG"
     gene            complement(1634..2353)
                     /locus_tag="VFJ67_07210"
     CDS             complement(1634..2353)
                     /locus_tag="VFJ67_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290214.1"
                     /translation="MLKKTALAAVVMALSFFTIGGCSSGGGGGGGPCPELNTGIEVCD
                     PETGGPFSLEIDNGFFPVVVGSETVLEGEDDEGTIIRVETTVPGDTEMVAGVNTRVLV
                     ETEFEDEVLVEQSRNFYAQAPDGTVCYFGEDVDIYEDGEIVSHDGAWRAGEDGNLPGI
                     QMPGDPEIGMIFQQEFAPGIAEDQTEILAFGEEIEVPAGMFSDTLTTEDCNPLDKTTD
                     LKFYVDGIGLAIDESAELISF"
     gene            complement(2752..3585)
                     /locus_tag="VFJ67_07215"
     CDS             complement(2752..3585)
                     /locus_tag="VFJ67_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290215.1"
                     /translation="MISTVALKELSGSARGAWRRIVYAALILLAGLAFGPTSDARDRH
                     DDKESKAAEIPFGAAEIRIETNSTDGDAGLQIFLDGEGWKQIRIEDPNGRLVFEVDDF
                     GKLRKLGSTELFMESNEPDFQEDMPLPEILKLLPAGVYDFEGTTIDGEELEGEAELTH
                     DLPCGPEVTSPGDGDTLDPDAAVVIDWEPVTNKIDTTSDTGECSDSTDIEIIGYEVIV
                     ELEDAEPPQKLDIFLPASVTSVTVPAELIVPGGSYKFEILAIEASGNQTITEGFFSTS
                     E"
     gene            complement(3711..>3974)
                     /locus_tag="VFJ67_07220"
     CDS             complement(3711..>3974)
                     /locus_tag="VFJ67_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7290216.1"
                     /translation="AGGRIDIEASRGEKEVEITVKDTGVGISEDELDNIWDRLYRGDK
                     SRSQRGLGLGLSLVKAIVGAHRGRVEVSSKPGSGSVFSVHLPA"
BASE COUNT          839 a         1216 c         1065 g          854 t
ORIGIN      
        1 gataaaaata aaagagctca gggagctcga cgaggcctgg gccaagctcg cgaagctgaa
       61 gacgcacaag aagtggggga aggagttcga gaagtacgta gtgtccggca ccgcgcgctg
      121 gcaggttttc aggatagtca acgactgacc gcccgcgccg ggccccgaac gagcgcggcg
      181 ggcagtccgt ggcccccgcc cgacagacgt gaacgtatta tataatctcg cccaggagga
      241 ttatatatgc ctttaccata ccccgtaatt gtaattcccg gcatcaccgc gacttatctg
      301 aaaaacgaat acgacctgcc gccggagtac gtctggacgg ttatgacgaa gaagtacgag
      361 cggctcgcgc ttcatcccaa cgacctacgc tacgaggtct cggagcccgc gagactgaaa
      421 cccgaccagc tgtacgaaat agcgtataag gaattgatcg aggagctccg ctataacctc
      481 cgctcggagg aggacaagca ggtgccggtc tttcccttcg gctacgactg gcggatgaag
      541 ctcgaagaca ttcaatccga gctcgattcc ttcgttcagg aggtgatcga ccgcactaag
      601 ctgctcaagc actactacag ggccgggtac caggacgatc ccaaggtaaa tctcgtcgga
      661 cactctatgg gcgggcttat aatagcgggt tatctcaaga gcaagggccg ccaggcccct
      721 gttcacaagg tcgtgacgtt cgcgacgccg tatcagggat ctttcgaagc cgtcataaag
      781 gtcacgacgg gcactgcgaa cctcggcacg tccccgccga gctcgcggga gagggaatcg
      841 gcccgcgtca cgcccgctct ctattatctt gttcccagct tcagcggcgg gctcgaagtg
      901 cccccgggcg tgccccagtc gctcttcgat gcgggcgcgt ggcagccgag catagtgcag
      961 tcgataaagg aatggatacg cctgaagggc cttccgaccc cggaccagga cgcgctcgct
     1021 gagcagatat tttcaagcat gctgaagtcg ggtaaaaacc accgcgcgaa tatagacacg
     1081 tttaaactat cggacgccgg catgactgct gacaggtggc tcgccgtggt cggcgtggac
     1141 tcagttacaa gggtgagact gaagatagtc aagaacggga ggttccccga attcgagttc
     1201 aagacctccg acagggacaa caagtggttc ccggccagga tcaccgaccc cgagtgcagg
     1261 ctcacgggcg acggcactgt ccccttcgag ggagccgtgc ctaaattcct cgcccccgaa
     1321 aacctcgtct gcgtcacgcc cggcgactac gggtactggg agatagggga caaggtgctt
     1381 accgaattcg ggggattcca cggcatcatg ccgaagatga acatgctcca caggctgctg
     1441 gtcaggtttt tcaccgacgc gccggataag cgcgacaaca cgtggggccg ccgcgctccc
     1501 ggagtagcag acggacagtg gaagccgccc ctctcgctca aggacaagac cctcgggtga
     1561 atgctcgcgg gcctgattac gggcaaccct atatcatcct gaacgaatga agcggctccg
     1621 gcgtccccaa aacttaaaac gagatcagct ccgcggactc gtctatcgcg agccctattc
     1681 cgtccacata aaactttagg tcggtcgtct tgtcgagagg gttgcagtcc tccgtcgtaa
     1741 gcgtatcgct gaacatccca gccgggacct ctatctcctc gccgaacgcg aggatctcgg
     1801 tctggtcctc ggctatcccc ggcgcgaact cctgctgaaa aatcatcccg atctccgggt
     1861 cgcccggcat ctgtatcccc ggaaggttcc cgtcctctcc cgcgcgccac gccccgtcgt
     1921 gagacactat ctccccgtct tcgtatatgt ccacatcctc tccgaagtag cagaccgttc
     1981 cgtcgggggc ctgagcgtag aagttcctcg actgctcgac caacacttcg tcctcgaatt
     2041 ccgtctcgac cagcacgcgc gtattgactc cggcaaccat ctcggtatcc cccggtaccg
     2101 tcgtttcaac cctgataata gtcccttcgt cgtcctcccc ctcaagcacg gtctcgctcc
     2161 ccacgacgac agggaagaac ccgttgtcaa tctcgagcga gaagggccct cctgtctcgg
     2221 ggtcgcagac ctcgatgccg gtgtttaatt cgggacacgg tcctccgccc ccgccgcctc
     2281 cgctgctgca acctcctatt gtgaagaacg ataaagccat tactacggcc gccaatgctg
     2341 ttttctttaa cattgtaata cctcctctct gaaactgttt acccacatga tagagaacga
     2401 agcttgcccc tccatttcag aaagattaca agtttgtaac gtttaaccat gaaagcagcg
     2461 aatttctgtc attgcgagac tccgtaggag ccgcggcaat ctcatttttt ttccttttaa
     2521 ttccctcccc ctctaggagg gaggggtagg gtgggggtgt acattaattt tcaatcattt
     2581 acataaaccc gccccattca tcgatacttg tcaggaacag ggcgggtgca ggtaatgagg
     2641 gatgagtcat gtaattgaat taaggggagg gactcccccc gcattcctca tagctgctct
     2701 catgctacgc cggagggagt ccccgtaata aaccagttct gatgtcagtg gtcattccga
     2761 agtcgaaaag aacccttcgg tgatcgtctg attcccgctc gcctctattg ccaggatctc
     2821 gaatttgtat gatccgcccg gcacgatgag ctccgcggga actgttaccg aagttacact
     2881 ggcgggaaga aaaatatcta gtttctgcgg gggctccgca tcttcaagtt caactattac
     2941 ttcataacca ataatctcga tgtcggtcga atcactgcac tcgccggtgt cggaagtcgt
     3001 atcgattttg ttcgtaacgg gctcccaatc gattacaacg gccgcgtccg ggtcgagcgt
     3061 atccccgtcc ccgggcgatg ttacttcagg cccgcacggc aggtcgtggg tgagctccgc
     3121 ttcaccctcg agctcttcac catcgatagt cgtcccctcg aagtcgtata cacccgccgg
     3181 caggagcttc aatatctcgg ggagcggcat gtcctcctgg aaatcaggct cgttgctctc
     3241 catgaagagc tccgtgctcc cgagtttcct cagcttgccg aagtcgtcca cctcgaatac
     3301 gagacggccg ttcgggtctt cgatccttat ctgcttccag ccttccccgt cgagaaatat
     3361 ctggagaccc gcgtcgccgt cggtggagtt cgtctcgatg cgtatctccg ccgcgccgaa
     3421 cggtatctcc gccgctttgc tctctttatc gtcgtgcctg tctcgcgcgt cggatgtcgg
     3481 accgaacgcg agccccgcga gcagaatcag cgcggcatag acgatccgtc tccacgcccc
     3541 tcgcgcgctt cccgataatt cttttaatgc tactgtgctt atcattacgc acctcctgaa
     3601 tctggattta cttccatgat acagggcgcg tgattgccgg aatatttcac gaacattaca
     3661 tcgcggaaat ggttgtgtga tgggaatgcg tgaagggaat gaataccgga ttaagcgggc
     3721 agatgcactg aaaagacgga gccggacccc ggcttgctcg atacctcgac acggcccctg
     3781 tgcgcgccga ctatggcctt aacgagactg agcccgagac cgagccccct ctgcgagcgg
     3841 ctcttgtcgc ccctgtagag gcggtcccag atgttgtcga gctcgtcctc agagatgccg
     3901 acgcccgtgt ccttaacggt gatctcgacc tctttttctc ctcggctcgc ctctatgtct
     3961 atccttccgc cggc
//
LOCUS       DASSJH010000330        12823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_513552, whole genome shotgun sequence.
ACCESSION   DASSJH010000330 DASSJH010000000
VERSION     DASSJH010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513552"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /gene="ilvN"
                     /locus_tag="VFJ67_07225"
     CDS             <1..526
                     /gene="ilvN"
                     /locus_tag="VFJ67_07225"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET7290217.1"
                     /translation="ITGGLLPVRHTITLLVDNESGVLSRIAGLFSARGFNIESLNVGE
                     TLDPETSRITLVTTGDDFIIQQIIKRFNNMVNVIKVSDLTDEVRVEREMVLVRMDARD
                     ETRAEILRTADIFRAKVVDVGPKSYTLELTGDKDKINGFIELLRPMGIKEIARTGTVA
                     MKRETKLMKRGEEK"
     gene            523..1539
                     /gene="ilvC"
                     /locus_tag="VFJ67_07230"
     CDS             523..1539
                     /gene="ilvC"
                     /locus_tag="VFJ67_07230"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7290218.1"
                     /translation="MKVYYDSDINLKKLKKRKIAIIGYGSQGHAHAQNLRDSGMDVAV
                     GLREGSGSWEKAKEAGFRVLPVDEAAKWADIVMILAPDTSQPGIYHGGISEGLEAGNA
                     VAFSHGFNIHYGQIVPPPNVDVFMVAPKAPGHTVRGQFAEGAGVPMLVAIHQDATGEA
                     KEIALAYAGAIGGGRAGIIETTFKDETETDLFGEQAVLCGGLTSLIMAGFETLIEAGY
                     PPEMAYFECCHEVKLIVDLIYEGGISNMRYSISDTAKYGDLTRGPRVVDEDAKKEMKK
                     ILTSIQNGEFAREWILENRAGRPVYNALLKKGEEHQIEGIGGELRKMMSSLFKKKLVD
                     KTKN"
     gene            1539..2222
                     /locus_tag="VFJ67_07235"
     CDS             1539..2222
                     /locus_tag="VFJ67_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HET7290219.1"
                     /translation="MERKRSPIATEGFTYLGVLAILAWAAAIFNFTLISIIFAVLAVI
                     TLFFFRDPEREVPDDPDSVVSPADGSVIGVENIYERDFLGAPMTRISISLALYDCHIN
                     RMPVRGRVAGTKYSPGSFNIAHMPDWLFSEGMKRKSDDNEKLSTLIETPDGEKIVVSQ
                     IAGFLARRIISYADIDMKFKKGERFGMIKFGSRVDVYLPEGCIIEVNVGNTVKAGESI
                     IAWLDGNRA"
     gene            2198..3025
                     /gene="pssA"
                     /locus_tag="VFJ67_07240"
     CDS             2198..3025
                     /gene="pssA"
                     /locus_tag="VFJ67_07240"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET7290220.1"
                     /translation="MARREQGLRRKKSKRQQVMPVLPNIFTTGNLSFGLLSILTSIEI
                     ASAMGAGTASEGWVFRRFWWAGAFIVISFFFDTLDGRLARFIKHESSFGLSYDSISDI
                     VSFGVAPAVLIYVWTLMDKGKLGLMAVIVYVVCAALRLARFNVQSGTVERFSFTGLPS
                     PMAAGLMVSPLMLIASLKIMPDERVLWYYLVAAPVIGLLMVSNVKYTKQPALRLGGPF
                     NALVVAAIIIAAVITNPEIMFIFLVYLYAVSGLVIHALRYLTGKPVVREETGPGEAG"
     gene            3176..6046
                     /locus_tag="VFJ67_07245"
     CDS             3176..6046
                     /locus_tag="VFJ67_07245"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7290221.1"
                     /translation="MKSGVGERLVFNVGKVSDRRRARISALSSFLAVILGIMVVSCHR
                     EEKKMDNESLKKETVNGITKYELDNGMTIILEENHSAPVVAVNVWVKTGSACETEGEY
                     GLAHVHEHMVFKGTDKRAVGEIARVIEGSGGDINAFTSFDETVYYVVIASRFMDTALD
                     VLSDTMENSTFDPNELNKELEVVVEEIRRGEDNPGRNLSEKMFSTAFTEHPYGRSIIG
                     TEQGVKSFTRKKVTDFYHKWYAPNNMALVVVGDFDTAKIEPRIEATFGKLHKRELPDC
                     DIKDEPEQKGMRAFVIDKPLQEGYFSLAFHTPNAKGEDTPAMDVLANILGGGESSRLY
                     RNIKEEKGLANNIYAYSYTPMREGIFAVGGSIDPSKSKEALKEVMKEIMRLKYEPVGD
                     VELSKVKLNIESDAIYTKETMQGQAQKLGYFEVETGDFRYEDEYLGKVRKVTPEDVKR
                     IADKYLTVDNLTAGFLFPTGQKDMTEAEIREIAKQASDEISGEYARNKGNGSAVAAGG
                     EELTEEIIVPSVGANAASKAASVNGTQPAEGESSEVKRYVLDNGIRVLIKENPGVPLF
                     AARAAFMGGVRYEDDKTQGMSNFVSRMFTRGTESRSAAQIAEEIESIAGEVQGFSGRN
                     SFGVTVETLSQNFDEAMDIFSDVILHPSFDSQEIERARREILSEINREGDNLLRTTVN
                     LFLATLYADHPYKYNPLGTVDTVTQFDGSDLREFYAKYVRPENMVITIVGDVDGNEAL
                     EVIKEKFGGMKKVASPIPPVSQVVPPSKVTEKIETKPDKAQTHIIMGFLAPPINSEDE
                     YAFEVLNTILSGQGGRLFIELRDKMSLAYTVTSFYTPGLEPGYFGVYIGCAPQKEQEA
                     VKAIKDQLELVLKDGVTDEEIKRAQNYLVGSFEIGLQQNSSQSAKITFDELYDIGWDE
                     YKRYPQEIYAVTKEDVQRAARKYIDLAKYTLVIVKPESATQG"
     gene            6627..7679
                     /locus_tag="VFJ67_07250"
     CDS             6627..7679
                     /locus_tag="VFJ67_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7290222.1"
                     /translation="MRANVFKDKNKIGLEEKPTPKAGPGEAVVRVTLTTICGTDVHIM
                     RGEYPVRQGQTIGHEPVGVIHELGNGVTGYKVGERVAINAITPCGMCEYCLSANWAQC
                     GGFLGGWKMGNTIDGCQADYVKIPSAMANLARIPDELSDEDVLLTTDIFSTGLSGAES
                     ADIQVGDMVAVFGQGPIGLGATAGAKLKGASLIIGVDTIPSRMDMAKKMGADVVIDFK
                     KTDPVKEILDMTNGRGVDVAIEALGTQTTLENCVKATRVGGTISSLGVYPADMANIGV
                     PNQAFGFGIADKNIVSTLCPGGKERMRRLMDLVRNERVNLRQLITHRFSLDEIEVAYK
                     LFSSQADGVIKVAIQP"
     gene            complement(7784..8170)
                     /locus_tag="VFJ67_07255"
     CDS             complement(7784..8170)
                     /locus_tag="VFJ67_07255"
                     /inference="COORDINATES: protein motif:HMM:NF037502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN family nuclease"
                     /protein_id="HET7290223.1"
                     /translation="MLGYLYCGILLPKENWSELIPDIEIFKLDSPWDLKDAKIDAPKM
                     KKITLRQFVRRIRNSLGHGNVIFHVPEKITDRNQKWAETTLEFSDINPRDSSDTFHAI
                     ISLDSLLKMVKQIHGIVYTNVKERLK"
     gene            8641..9591
                     /locus_tag="VFJ67_07260"
     CDS             8641..9591
                     /locus_tag="VFJ67_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HET7290224.1"
                     /translation="MFEVIVVIIGLSLFEIISSIDNAVVNAHVLATMGDRFRRFFLIW
                     GMLIAVFILRGVLPFLIVWIANPHLSFIELFKFTFSGDSKQIEESLAESKAYLLVGGG
                     MYLFLVFLAWLFLEEKKYTFLVEHFIHRHSVWFYAITSIFFTVIIYISLQFDPFLAFA
                     ASIGTTAFFITDGFKKNAEEKEKELLDPHMSGWSKVFYLEVLDASFSIDGVIGAFAFT
                     MSVPLILIGNGIGAFVVREVTVRGINWISKYAYLKNGAMYSIGMLGAIMILESFGEEI
                     PFWIAPLNTVILLAIFLFLSWREIKLAEKLEAEGKGGSAA"
     gene            9780..9856
                     /locus_tag="VFJ67_07265"
     tRNA            9780..9856
                     /locus_tag="VFJ67_07265"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9814..9816,aa:Lys,seq:ctt)
     gene            9860..9944
                     /locus_tag="VFJ67_07270"
     tRNA            9860..9944
                     /locus_tag="VFJ67_07270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9894..9896,aa:Leu,seq:tag)
     gene            complement(10386..10619)
                     /locus_tag="VFJ67_07275"
     CDS             complement(10386..10619)
                     /locus_tag="VFJ67_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7290225.1"
                     /translation="MSKAVFYHAGCPVCVSAERWVAEALDPEKYEVEVVHLGREKGRI
                     PEAEAAGVKSVPALVMNGQTYHINFGASISDLK"
     gene            10717..11286
                     /locus_tag="VFJ67_07280"
     CDS             10717..11286
                     /locus_tag="VFJ67_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET7290226.1"
                     /translation="MNEGAKIASLIERLGNLLRAGERSSGAGLLPVHVQMLSYLSVCN
                     RYSDTPAAVTEFVGATKGTTSQSIGVLERKGYLRKRSDDKDGRVIHLGLTAKGRRFVK
                     NNFPPPEFGASVVEMKPAERESLARLLTGLLVSLQRRNGGRMFGVCRTCRYFRKDALP
                     GSHQCGLTLEPLSDDESVLICREHELPAA"
     gene            11671..12780
                     /locus_tag="VFJ67_07285"
     CDS             11671..12780
                     /locus_tag="VFJ67_07285"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET7290227.1"
                     /translation="MTRNGTPAEHNARSNAKIRFSPNTEFEKELKKRVSVYFEENDLS
                     KKDNGRMYLKTFLAVGWVAGSYALLVFAGLPVWGLIIAAISLGVALNALSFNVMHDAL
                     HGAYSERPWVNRLMGHFLDLIGGSSYFWYRKHNYFHHSYPNIAGHDDDIEAGIFARLS
                     PHHKRYFFHRYQHIYIWPLYGFLAVKWHLFDDFYTFFTKKINGHEIDRPGGWDAAIFF
                     GGKLFFLTMAFVIPLFFYPVLYVAVFYMLVAFLQGLIMSVVFQLAHCNEDAGFPMHSG
                     ESKQMDTSWMVHQLSTTTDFDKDNHFLTWYLGGLNYQVEHHLFPKICHVNYPALSRIV
                     EETCREYSVTYNAYKSFFSGLRSHYNWLRLLGQPG"
BASE COUNT         3125 a         3283 c         3662 g         2753 t
ORIGIN      
        1 cataaccgga ggtttattac cagtgaggca cacgataacg cttcttgtcg ataacgaatc
       61 cggcgtcctt tcgaggatcg ccggtctctt cagcgccagg ggtttcaaca tcgagagtct
      121 gaacgtcgga gagacgctcg accccgaaac ttcgcgcata accctcgtca cgaccgggga
      181 cgatttcata atccagcaga taatcaagag attcaacaac atggtgaacg tgataaaggt
      241 gagcgacctg acggacgagg tgcgcgtgga gagggagatg gtgctcgtgc gtatggacgc
      301 gagggacgag acgagggcgg agatactgag gacggccgat atattcaggg ccaaggtcgt
      361 cgacgtgggg cccaagtcct atacgcttga acttaccggg gacaaggata aaataaacgg
      421 ttttatcgag ctcctgaggc ccatgggaat taaggagatc gccaggacag ggaccgtcgc
      481 catgaagcgc gagaccaaac ttatgaagag aggagaggag aaatgaaggt ttattacgac
      541 agcgacatca acctcaagaa gctgaagaag agaaagatcg ccataatcgg ctacggaagc
      601 caggggcacg ctcacgccca gaatttgagg gacagcggga tggacgttgc cgtcgggctg
      661 agagaaggga gcgggagctg ggaaaaggcg aaggaagcgg ggttcagggt tctgcccgtg
      721 gacgaggccg ccaaatgggc cgacatcgtc atgatactcg cccccgacac gagccagccc
      781 ggtatatatc acggaggcat atcggagggg ctcgaagccg ggaacgcggt cgctttcagc
      841 cacggtttca acatccatta cggccagata gtgccgcctc cgaacgtgga cgtgttcatg
      901 gtggccccga aagcccccgg ccacacggtg aggggacagt tcgccgaagg cgcgggcgtg
      961 cccatgcttg tcgccatcca ccaggacgca acgggagagg ccaaggagat tgcgcttgcc
     1021 tacgcgggag ccataggcgg agggcgcgcg ggaataatag aaacgacttt caaggacgag
     1081 accgaaaccg acctcttcgg ggagcaggcc gtcctctgcg gcggtcttac ctcgctcatc
     1141 atggccgggt tcgaaacgct gatcgaggcc ggatacccgc ccgagatggc gtacttcgag
     1201 tgctgccacg aggtgaagct catcgtcgat ctcatatacg agggcggcat atccaacatg
     1261 cgctactcga taagcgacac ggcgaaatac ggcgacctca cgaggggccc gagggtggtc
     1321 gacgaggacg ccaagaaaga aatgaaaaag atccttacct cgatccagaa cggcgagttc
     1381 gcgagggagt ggatactcga gaacagggcg ggacgccccg tttacaacgc actcctcaag
     1441 aaaggggaag agcaccagat agagggcatc ggcggcgagc tccgcaagat gatgagctct
     1501 ctattcaaga agaagctggt cgacaagact aagaactgat ggaacgaaag cggtctccga
     1561 tagccaccga ggggtttacg tacctcggcg tactcgcaat actcgcctgg gcggccgcta
     1621 tcttcaactt cacgctaata tcgattattt tcgccgtgct cgcggtcatc acgctcttct
     1681 ttttccgcga tccggagcga gaggttccgg acgacccgga ctccgtcgtt tcgcccgccg
     1741 acgggagcgt gatcggggtc gagaatatat acgagaggga cttcctgggc gccccaatga
     1801 cgaggataag catctcgctc gccctctacg actgccacat caacaggatg cccgtgaggg
     1861 gcagggtggc gggaacgaaa tactcccccg gcagcttcaa tatagcgcac atgcccgact
     1921 ggctcttctc cgaagggatg aagaggaaat cggacgacaa cgagaaactc tcgaccctca
     1981 tcgagacgcc ggacggggaa aagatcgtcg tatcccagat agccgggttc ctcgcgagga
     2041 ggataatctc ctatgcggac atcgacatga agttcaaaaa aggcgaaagg ttcggcatga
     2101 tcaaattcgg atcgagggtt gacgtctacc tgcccgaagg atgtataata gaagtgaacg
     2161 taggcaacac tgtaaaagcc ggggagagca taatagcatg gctagacggg aacagggctt
     2221 gagaaggaaa aaatcgaaga ggcagcaggt aatgcccgtc cttccgaaca tattcacgac
     2281 ggggaacctc tccttcggac ttctctccat actgacctcg atagagatcg cctccgcgat
     2341 gggtgcgggg accgcttccg aggggtgggt cttcaggagg ttctggtggg cgggcgcttt
     2401 catagtcata tcgttcttct tcgacaccct cgacgggcgg ctggcaaggt tcataaagca
     2461 cgagagcagc ttcggcctgt cctacgattc aatctcggac atcgtttcgt tcggcgtcgc
     2521 ccctgccgtg ctcatttacg tctggacgct catggacaag gggaagctcg gcctcatggc
     2581 ggtgatcgtc tacgtcgtct gcgcagcgct caggctcgca cggttcaacg tgcagtctgg
     2641 cacggtcgag aggttcagct tcacggggct cccgagcccg atggccgcgg gcctcatggt
     2701 atcgccgctg atgcttatcg cgtcgctcaa gataatgccc gacgaaaggg tgctctggta
     2761 ctacctcgta gcggcgccgg taataggcct cctcatggtg agtaacgtta aatacacgaa
     2821 acagcctgcg ctcaggctcg gcgggccgtt caacgccctg gtggtggccg cgataatcat
     2881 cgccgccgta attacgaacc ccgaaataat gttcatattc ctcgtttacc tctacgcggt
     2941 ctcaggactc gtcatccacg ctctcaggta cctgaccggg aagcctgtcg tacgggaaga
     3001 aacgggtccc ggcgaagccg gctgattaat tagcactcta agtgactgta aatactgcac
     3061 atattgcacg gggcggactt tccctctttg atagggggat aattcccgtt atcttattac
     3121 gggcggaaaa ccccacgatc cgatagccaa atacgaaagc agagcggagt cgccgatgaa
     3181 atcaggtgtt ggagagaggc ttgtctttaa cgtaggtaaa gtttctgaca ggaggcgcgc
     3241 ccggatttcg gcattgtcgt cgttccttgc cgtcattctg ggtattatgg tagtttcttg
     3301 ccacagagag gagaagaaaa tggataacga aagcctgaaa aaagagaccg tgaacggcat
     3361 aacgaaatac gagctcgata acggaatgac cattatcctg gaggagaacc attcggcccc
     3421 tgtggtggcg gtgaacgtgt gggtgaagac agggagcgca tgcgagacgg aaggggaata
     3481 cggcctagcg cacgtccacg agcacatggt gttcaagggg accgacaaga gggcggtcgg
     3541 agagatagcg cgggtgatag aggggagcgg tggggacata aacgcattca cgtcgttcga
     3601 cgaaacggtc tactacgtag tcatagccag caggttcatg gatacggcgc tggacgtcct
     3661 gtccgacacc atggagaact ccactttcga cccgaacgag ctcaataagg agctcgaggt
     3721 ggtggtcgaa gagataaggc ggggtgagga caaccccggc aggaacctga gcgagaagat
     3781 gttctcgaca gccttcaccg agcacccgta cgggaggtcc atcataggga ccgagcaggg
     3841 ggtgaagagc ttcacgagga agaaggtcac ggacttttat cacaagtggt acgcgcccaa
     3901 taacatggcc ctcgtcgtgg tcggtgattt cgatacggcg aagatcgagc ccaggataga
     3961 ggcgacgttc gggaagctcc acaagaggga gctccccgat tgcgatataa aggacgagcc
     4021 cgagcagaag ggcatgagag ccttcgtcat cgacaagcct ctccaggagg gatatttctc
     4081 gctcgcgttc cacacgccga acgcgaaggg cgaggacacc cccgcaatgg acgtgcttgc
     4141 caacattctt ggcggcgggg agagctcgag gctctacagg aacataaagg aagaaaaggg
     4201 cctcgctaac aacatatacg catactcata caccccgatg agggagggca ttttcgccgt
     4261 aggcggcagt atcgacccct cgaaatcgaa ggaagcgctc aaggaagtta tgaaggaaat
     4321 aatgaggctc aagtacgagc ccgtagggga cgtggagctc tcgaaggtaa agctcaacat
     4381 cgagagcgac gcgatataca cgaaagaaac catgcagggg caggcgcaga agctcggcta
     4441 cttcgaggtc gagacaggcg acttcaggta tgaggacgag tatttaggca aggtgaggaa
     4501 ggttacgccc gaggatgtaa agaggatcgc ggacaagtat cttactgtcg ataatctcac
     4561 ggccggcttc cttttcccga cgggacagaa agacatgaca gaggccgaga taagggagat
     4621 cgccaaacag gcgtccgacg aaatatccgg ggaatatgcg aggaataaag ggaacggctc
     4681 ggcagtcgcc gcaggaggcg aggagcttac cgaggagata atcgtccctt cagtgggagc
     4741 gaacgccgcc agcaaggcag cgtccgtgaa cgggacccag cccgccgaag gagagtcgtc
     4801 cgaagtaaag agatacgtcc tcgataacgg cataagggtg ctcatcaagg agaaccccgg
     4861 cgttccgctc ttcgcggcaa gggccgcctt tatgggcggc gtaaggtacg aggacgacaa
     4921 gacgcagggg atgtccaatt tcgtctcgcg gatgttcacg agggggaccg aatcccggag
     4981 cgccgcgcaa atagcggagg agatagaatc gatcgcgggc gaggtgcagg ggttctccgg
     5041 gcggaacagc ttcggcgtca ctgtcgagac gttaagccag aatttcgacg aggccatgga
     5101 catcttctca gacgtgatac tccacccgtc gttcgactcg caggagatcg agcgggcgcg
     5161 gcgggagata ctgtccgaga taaacaggga gggggacaac cttctgagga ctacggtcaa
     5221 cctgttcctc gccacgcttt acgcggatca cccttacaaa tataatcctc tcggcaccgt
     5281 ggatacggtt acacagttcg acggcagcga cctgagggaa ttctacgcaa aatacgtcag
     5341 gcccgagaac atggtcataa ccatcgtggg cgacgtggac gggaacgagg ctctcgaagt
     5401 gataaaggag aaattcggcg gcatgaagaa ggtcgcgtcc ccgataccgc ccgtgagcca
     5461 ggtcgtaccg ccctctaaag tcacggaaaa gatagagacc aagcccgaca aggcgcagac
     5521 gcacatcata atgggtttcc tcgcccctcc cataaacagc gaggacgagt acgcgttcga
     5581 ggtgcttaac accatcctct ccgggcaggg cgggaggctc ttcatcgagt tgagagacaa
     5641 gatgagtctc gcgtacacgg tcacgtcttt ctataccccg ggtctcgagc ccggctactt
     5701 cggggtgtac ataggatgcg cgccgcagaa ggagcaggag gccgtcaagg ccatcaagga
     5761 ccagctcgag ctcgtgctca aggacggggt gacggacgag gagatcaagc gcgcccagaa
     5821 ttacctggtc gggagcttcg agatcgggct ccagcagaat tcctcgcagt ctgcgaagat
     5881 aaccttcgac gagctgtacg acatagggtg ggacgagtac aagagatacc ctcaagaaat
     5941 ttacgccgtc acgaaagagg acgtgcagcg cgcggccagg aaatacatag acctcgccaa
     6001 gtacacactc gtgatcgtaa agcccgagag cgcgacgcag gggtaggagc gagccgcttc
     6061 acgccacggg accccgctac acaactccta ttaccggtga tagccgctat ccggctcgaa
     6121 tcaccggcgc tctgaaaaat caccgtaact caccgcgggg agccgcgggc ttccgccctt
     6181 gaaatctcga attgtattag tgtataatgg ggtgcttaaa gagcccgggg ggacaaatcc
     6241 aaactcctaa tgtctcgtta aaggaggatt tgataaaaga ggtatggaga gtctcccccc
     6301 ccggacccaa actcgactat tacgtgtaat agtatagcag actttttccg gctgtaatca
     6361 tttttcgttc ccgctgaccg attttcccgg aaatattatt tccgcccgga taattgtata
     6421 taagacccgg ccgggtgaac ggttccgtat gccgctccga tcgcatatat ctaatgtagc
     6481 gtattaggac acaaaccgaa ttcatttcat acttccttaa taaaaagaat atactattta
     6541 gagcggatag gggtgtgccg gactctggca cggcatttgc tcctaataat aaatgataaa
     6601 ttcaagcaag cgggaggcat agtaaaatga gagcaaacgt attcaaggac aagaacaaga
     6661 tcggtctcga ggagaagccc acgccaaagg cggggccggg agaggcggta gtaagggtga
     6721 cgctgaccac tatttgcgga acggacgtgc atataatgag gggcgagtac ccggtcaggc
     6781 aggggcagac gatagggcac gagcctgtgg gagtgataca cgagctcggg aacggcgtta
     6841 cggggtacaa ggtaggtgaa agggtcgcga taaacgcgat cacgccgtgc gggatgtgcg
     6901 aatactgcct gtcggcgaac tgggcgcagt gcggcgggtt cctgggcggg tggaagatgg
     6961 ggaacactat agacggttgc caggcggact acgtgaagat accgagcgcg atggctaacc
     7021 tggcgaggat accggacgag ctttcggacg aggacgtgct cctcacgacg gacatcttct
     7081 cgacaggtct ttcgggtgcg gagagcgcag acattcaggt gggcgacatg gtggccgtgt
     7141 tcggtcaggg gcctatcggc ctgggcgcga cagcgggcgc gaagctgaaa ggggcctcgc
     7201 tcatcatagg cgttgacacc ataccctccc gtatggatat ggctaagaag atgggggccg
     7261 acgtcgtcat cgacttcaaa aagaccgacc ccgtgaagga gatactcgac atgactaacg
     7321 gcaggggcgt ggacgtcgcg atagaggcgc tcggcactca gacgacgctc gagaactgcg
     7381 tcaaggcgac gcgcgtgggc gggactatat cgagcctcgg ggtttacccc gcggacatgg
     7441 ccaatatagg tgtcccgaac caggcattcg ggttcggtat agcggacaaa aacatcgtct
     7501 cgacgctctg cccgggaggg aaggagagga tgcggcggct catggacctc gtcaggaacg
     7561 agcgcgttaa cctccgtcag ctcatcacgc acaggttctc gctggatgaa attgaggtag
     7621 cctacaagct cttctcttcc caggccgacg gtgtgataaa ggtggcgatc cagccgtaag
     7681 ggagattatg tcttatggtc tgttaaaagt gtgcatactg atgtggatat ataaaatagc
     7741 caaatattca gtttggtttt agatattatt tcgtgaaatc aatttattta agtcgctctt
     7801 tgacatttgt atagacgata ccgtgtattt gtttaaccat tttcaagaga ctatccaaag
     7861 atataattgc atgaaatgta tcagaagaat ctcttgggtt aatatcacta aattctaaag
     7921 ttgtttcagc ccatttttga tttctgtcag taattttttc tggaacatga aaaatcacgt
     7981 ttccgtggcc gagtgagttt cttattcgtc taacaaactg cctgagagtg atttttttca
     8041 tttttggcgc atcaatcttg gcgtctttca ggtcccaggg tgaatctaat ttgaatattt
     8101 caatatctgg tattaattcc gaccaatttt cttttggcaa tagaataccg caatatagat
     8161 atccaagaat tgcaccaaga cttcaaggaa tttttctttt tgcgcctgtt gtttttagcc
     8221 attcatcgta aaactttcct acagagatat catttcgtct ctttgaaaat attatttcac
     8281 taatagactc taaaaaactt aatgataaca ttgcgtccgt gacaaaatct atagaatatt
     8341 tttcttactc gaattctttg tcgacgttgt tggtcatatt tcttttatcg ccataatcat
     8401 acatatgcat taagtaaatg aagtatatag tagcaacagg atattatttt attgattagt
     8461 aaatcatcta cattataggt gttataattt gtgctttgag ttttagcatt cttgatagtt
     8521 gccaacgcct caggtcttgt ttatatatac ccggtttaat aagactgtat gtttcccagt
     8581 cttaattttt tcttgaaatt taagcctgat agtgaagaat aatagggaat ttctgaagcc
     8641 atgttcgaag tcatagtagt cataataggg ctgagcctgt tcgagatcat atcgagcata
     8701 gacaacgccg tcgtgaacgc tcacgtgctc gccacgatgg gggaccggtt caggcggttc
     8761 ttcctcatat ggggtatgct catcgccgtg ttcatattga gaggagtgct accttttctc
     8821 atagtatgga tcgccaaccc tcatctctcg ttcatcgagc ttttcaaatt cacgttctcg
     8881 ggggacagca agcagatcga ggagtcgctt gccgagtcga aggcgtatct gctcgttggc
     8941 ggcgggatgt acctctttct cgtttttctc gcatggctct ttctcgagga gaagaaatac
     9001 acgttcctag tcgagcattt catacacagg cattcggttt ggttctacgc cataacgtcg
     9061 attttcttta ccgtgatcat atatatatcg ctccagttcg acccgttcct cgcgttcgcc
     9121 gcatcgatcg gcacgaccgc ctttttcatc accgacgggt tcaagaagaa cgccgaggag
     9181 aaggagaaag agctgctcga cccgcacatg agcgggtgga gcaaggtttt ctatctggag
     9241 gtgctggacg cctcgttctc catagacggt gtgataggcg cgttcgcatt cacgatgtca
     9301 gtgccgctga tactgatcgg aaacggcatc ggcgcattcg tcgtgaggga agtcacggtg
     9361 cgcggtataa actggatatc gaagtacgcc tatctcaaga acggcgcgat gtattcgata
     9421 ggaatgcttg gagccattat gatactcgag agcttcggcg aagaaattcc cttctggatc
     9481 gcacccctaa atactgtgat actgttggcc atattcctct tcctttcctg gcgggagata
     9541 aaactcgccg agaagctcga agcggaaggg aagggcggga gcgcggctta aaataatcgt
     9601 cgcaggcatt gtctccggtc ccgttcgatc cgtaagcact aaaaattcaa aacgttgatt
     9661 ttgtgtgtaa ggtcattata ataaaccttt tctcgaaata tcaggcagtc aggccgtttg
     9721 ccggcttact ccaaatttat actgatttaa ggtattctaa gtacgcgtaa ccgtggtgtg
     9781 ggctgttagc tcagtctggc agagcagatg actcttaatc atcgggccgt gggttcgatt
     9841 ccctcacagc ccaccacatg cgagagtggc ggaactggca gacgcgcagg acttagaatc
     9901 ctgtacctaa ccgtgtgggg gttcgactcc cccctctcgc accaattatt cctccaccga
     9961 attgatgttt caatccgcgc ccgctgaatt ttcttttcgc cagatctatc atgcctgggg
    10021 ctatatccac agccgtcacg tgcgcatggg gaaaacgtct tatcagcaac tccgtgagat
    10081 gccccgtttc gcagcctacg tccaggatag cgccgggctt gtctccctcc gccggatccg
    10141 cgaattccat gagggttgaa gccatttttt tctggacatg ggcgtacagg tcgtaattcc
    10201 ctgcgtttgc gctgaagtgc attctcacaa gatgcttgtc gggcatgatc gtccggtcac
    10261 ttgaaagggg agcggggtta aatcctaccc gccccattat gccggagcaa cagcggagga
    10321 tacgcagcga aagggctgcg gtgtttgtcc gcacgggccc ttccgcatgg taatgggcaa
    10381 tccccttatt tcagatcgct tatcgaagcg ccgaagttga tgtggtacgt ctgcccgttc
    10441 atcacgaggg cggggaccga ctttacgccc gccgcttccg cttcggggat ccttcccttc
    10501 tcccggccga ggtgcacgac ctctacctcg tatttctccg ggtctagcgc ctccgctacc
    10561 catctctctg cgctaacgca taccggacac cccgcgtggt agaacaccgc tttactcatg
    10621 gcaattacct ccgttggaaa ttggtatcga ctcgatacta ttattctctg acgccgggat
    10681 attgtcaagc tggtttcgat tcgatacaat agtctcatga acgaaggggc gaaaatagcc
    10741 tcgcttatcg agcgtctggg taatttgctg agagccgggg agaggtcctc aggcgcgggt
    10801 ctcctgcccg tgcacgttca gatgctctcc tatctctccg tctgcaacag gtacagcgac
    10861 acccccgccg ccgtaacgga gttcgtgggt gcgacgaagg ggacgacttc ccagagcatc
    10921 ggggtgctcg agaggaaggg gtatctccgg aagcgctcgg atgataagga cggaagggtc
    10981 atacacctgg gcctcaccgc gaagggcagg cgtttcgtca aaaacaattt cccgccgccc
    11041 gagttcggcg cttcggtggt cgagatgaaa cccgccgaga gggaatccct cgcgcggctc
    11101 ctcaccgggc tcctcgtctc gctccagcgt aggaacggtg ggcgcatgtt cggcgtctgc
    11161 cgcacttgcc gctacttcag gaaggacgct ctcccgggct cacaccagtg cggcctcaca
    11221 ctcgagccgt tgtccgatga cgagagcgta ttgatttgca gagagcacga gctgcccgcc
    11281 gcctgaagag gaagaggagc cgattcatgc ggaaacgcgg ataaaggcac gccggaatgg
    11341 atgaatcatt taaagtgctg aaaaatcgtc aaaaacatac attgtgtgtc ctttgtgctc
    11401 ggctgtttaa gtgttatact atccccctaa taaattcacc cttcatacgg tcatccggat
    11461 ctattcgttc agatacaggc tcgagattga ttacattgca gggttaaaag gggaattcgg
    11521 caggtccggt tgatcgctgt ataaaagtac cgagaacgtt tccggtcagc tccatattca
    11581 aaacggccgg aactcttccc gattcgatgt gataatcggg aacacgtact caatacattc
    11641 gaacgaggat caccgcctgt caatactacg atgactcgga acggcacacc ggcggaacat
    11701 aacgcgcgtt caaacgcaaa gattcgtttt tcaccgaaca cggagttcga gaaagagctc
    11761 aagaagaggg tctcggttta cttcgaggag aacgacctct cgaaaaagga caacggcagg
    11821 atgtacctca agaccttcct cgcggtcggc tgggtggcgg gttcgtatgc actcctggta
    11881 ttcgccgggc tcccggtctg gggactcata atcgccgcca tatcgctcgg cgtcgcgctg
    11941 aacgcgctct ccttcaatgt gatgcacgac gctctccacg gggcttattc ggagcgtccc
    12001 tgggtcaaca ggcttatggg gcacttcctc gacctcatcg gagggagctc ctacttctgg
    12061 tacaggaagc acaactactt ccatcattcc tatcccaaca tcgcggggca cgacgacgac
    12121 atagaggcgg gtatattcgc gcgcctgagc ccccaccaca agaggtattt cttccaccgc
    12181 tatcagcaca tatatatatg gcccctatac ggtttcctcg cggtcaaatg gcatctgttc
    12241 gacgacttct acacattctt cacgaaaaaa ataaacgggc acgaaataga ccgtccgggg
    12301 ggatgggacg ccgctatctt tttcgggggc aagctcttct tcctgacgat ggctttcgtg
    12361 ataccgctct tcttctatcc ggttctttac gtggccgtat tctacatgct ggtggcgttc
    12421 ctccaggggc tgataatgtc ggtcgtgttc cagctcgccc actgcaacga ggacgcgggc
    12481 ttccccatgc actcggggga atcgaagcag atggatacga gctggatggt ccatcagctg
    12541 agcaccacta cggatttcga caaggacaac catttcctaa cgtggtacct gggcgggctc
    12601 aactaccagg ttgagcacca cctgttcccg aagatatgcc acgtaaacta cccggcgctc
    12661 tcccgcatcg tcgaggagac gtgcagggaa tacagcgtca cgtataacgc ctacaagagc
    12721 ttcttctccg gcctccggtc tcattacaac tggctcaggc ttctcggcca gccggggtag
    12781 gatgcttgag ggagagcttg aagaattgcc gtgagcacgg tgt
//
LOCUS       DASSJH010000331         4656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1157665, whole genome shotgun sequence.
ACCESSION   DASSJH010000331 DASSJH010000000
VERSION     DASSJH010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157665"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VFJ67_07290"
     CDS             complement(<1..1096)
                     /locus_tag="VFJ67_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7290228.1"
                     /translation="MDDVPLSYNFLLAVFFLLLSGFFSASEGSLFSLSRHQRERLKKE
                     GRKSSRLIEKLLHEPYKLIITILFADEVVNVAYTSLIGLTVNEVLRGYSERTVTILSI
                     VIASPSLLLLGEIGPKTLGVKYPRIIAGIISYPLHLFHLIITPVRWVLVILSIGFTRI
                     LGGRIEYEHSKEFTPEEVKVLVGLGSEEGVITDIEKKLVGSLFKLEEVPAYKIMTPSI
                     DCFLLPANLSRKDAVYEIKKRGFSRTPVYEGDKNNIVGVLYAKDLISFDLSDEAGLKG
                     LLKPPYFIPRTKMAFDLLKELQHERKHIAIVVDEYGRFDGLVTMEDILEELFGEIEDE
                     RRVVKRQQVRWEGEALIIPGSMKIEEFNDTF"
     gene            complement(1097..2080)
                     /gene="thiL"
                     /locus_tag="VFJ67_07295"
     CDS             complement(1097..2080)
                     /gene="thiL"
                     /locus_tag="VFJ67_07295"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET7290229.1"
                     /translation="MINELSALRLIEEQFPSPIGCVEVGIGDDSAALRVSPGRLLLAT
                     TDCQVEDVHFQKGHITPEGLARRSVAVSVSDVGAMGGVPKFILASLGFSGDENEAFLR
                     KLIDGFRSSEKEFGVKLVGGNLSSSEKLFLDVTSLGEVEPEHLVRRSGASPGDVIYVS
                     GTVGDSALGLRLLKDGRTSGKYSYLVDRHLNPEPRLALGRRLALAGAATSMIDVSDGL
                     TLDLERITVQQGVGARIDLDMIPVSDEYARHITGYADEFFADALGGGEDYELLFTSSP
                     GRKDDVREISAALDILITEIGEVVSDPALTVLDRDGKEVSIKRKGFIHFGA"
     gene            2480..4030
                     /locus_tag="VFJ67_07300"
     CDS             2480..4030
                     /locus_tag="VFJ67_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290230.1"
                     /translation="MTGNVDGLEKRVYPRISNLTLVILALLLVKLAVQLYLYRIGFLS
                     VSADEFSRGITAARWSLDGTLSTTIRMGDWLPFETYINGLSLKVWDNVIWTPRITAFI
                     FSCFLLVYFIKLVQHLFGLLPVTLLAGLLLVFNPWFIWLSGTPMLEVYYLAPFVAGLY
                     YVSKWISGRRGAYLVIGGLLFFLSTGFHSQSWILVNVVNLCLCFFAWSMLRQRDYGGL
                     VMLAGFFMLGNLFIIIYLPSDYLATGQWLAMFHRHAQGTIEYYGGYEIGLFHKLSYYP
                     ELVVKSSKLVWIFFPIGLYRLHGEPGIRIKLFPFAVGLGALFFYSVFNVLSVPASAAP
                     GRYSLPFFILFVPYAALGIYALIEGGGYFADVRIRRVAAAILVIFILGLNFMKAIDFE
                     VKSAKAAVSIGRYLKESMEKDGADPKETVMVELSYWDYLYVELAARHFDRVRYDRKES
                     NEYTGIPSDLISMGDDDILRYMKSGDTKLIAVKDEAIKKRLDSLAFMHKIKDLDVWAV
                     YDIDLKHD"
     gene            complement(4158..>4656)
                     /locus_tag="VFJ67_07305"
     CDS             complement(4158..>4656)
                     /locus_tag="VFJ67_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7290231.1"
                     /translation="YESFIHPITILSGLPSAGFGALIVLMIFGVELNVYAFVGLIMLI
                     GIVKKNAIMQIDFALDAQRSEGKSPVEAIYQGCLIRFRPIMMTTMAALLGALPLALGH
                     GAGAESRRPLGLAVVGGLLFSQLITLYLTPVIYIYMDKIQSYVPRLSLARLRGKRTGE
                     SYASR"
BASE COUNT         1050 a         1322 c         1155 g         1129 t
ORIGIN      
        1 ggaacgtatc gttaaactcc tctatcttca tgctacccgg tatgatgagg gcctcgcctt
       61 cccacctgac ctgctgcctc ttgaccactc tcctttcgtc ctctatctcg ccgaacagct
      121 cctccagtat gtcttccatc gtcaccagac cgtcgaagcg gccgtactcg tccacaacaa
      181 tcgcaatgtg cttgcgctcg tgctgaagct cttttaacaa gtcaaaagcc attttcgttc
      241 tcggtatgaa ataagggggt ttcagcaagc ccttcagccc cgcttcgtcc gagaggtcga
      301 acgagataag gtccttggca tagagcacgc cgacgatatt attcttatcg ccctcgtaaa
      361 caggcgtcct cgaaaacccc cgcttcttta tctcgtaaac cgcgtccttc cgcgacaggt
      421 tcgcgggaag gaggaagcag tcgatgctcg gggtcataat tttataggca gggacctctt
      481 cgagcttgaa gaggctgccg acgagctttt tctctatatc ggtaatcaca ccctcttcgc
      541 tgcccaaccc tacgagcacc ttgacctcct cgggcgtgaa ttccttgctg tgctcgtact
      601 cgatcctgcc gccgagaatc ctcgtgaaac cgatcgagag tatgacgagg acccacctga
      661 cgggcgtaat aatcagatgg aacagatgga gcgggtatga gattatgccg gcgatgattc
      721 tcggatactt tacacccagc gtcttgggtc ctatctcacc cagaagcagg agactcggag
      781 aggcgataac tattgaaagt atggtaacag tccgttccga ataacctctc aggacctcgt
      841 tgaccgtgag accgatgagg ctcgtgtagg cgacgtttac gacctcgtct gcgaacagga
      901 tagttattat gagcttatag ggctcgtgga ggagcttttc tatgagcctc gagctcttcc
      961 ttccctcctt tttgagcctt tcacgctgat gcctgctcag agaaaaaagc gatccctcgg
     1021 aggcgctgaa aaagcccgag agcaggagaa agaatacggc taataaaaaa ttgtaactta
     1081 acggtacgtc gtccatttaa gcgccgaagt gaataaaccc ttttctttta atagagactt
     1141 cttttccgtc ccgatcgagc acggtgagtg cggggtcaga caccacctcg ccgatctccg
     1201 ttattagtat atccagtgcg gctgaaattt ccctgacatc atccttccgc ccgggggatg
     1261 atgtgaagag aagctcgtaa tcctcccctc caccgagcgc atcggcaaaa aattcgtccg
     1321 cgtaaccggt gatatgcctc gcatactcgt cggaaacggg gatcatgtcg aggtcgatcc
     1381 tcgcccccac gccctgctga acggttatgc gctcgaggtc gagcgtgagt ccgtcgctca
     1441 cgtctatcat cgaggtagcc gcccccgcaa gcgcgagcct cctcccgaga gcgagcctgg
     1501 gctcgggatt caaatgtcga tcgaccagat acgagtattt acctgaggtc ctgccgtcct
     1561 taagcagcct cagccccagc gccgagtccc cgacggtccc gctcacgtat atcacgtcac
     1621 ctggtgaagc gccgctcctt ctgacgagat gctcgggctc gacctcgccc agcgaggtga
     1681 cgtccagaaa aagtttttcg gacgagctga ggttccctcc cacgagcttc actccgaact
     1741 ctttctcgga agacctgaat ccgtctatga gcttcctcag aaacgcctcg ttctcgtccc
     1801 cggagaatcc gagtgacgcg aggatgaatt tcggaacgcc gcccatggcg cccacgtcgc
     1861 taaccgaaac ggcgaccgat cttctcgcga gcccttcggg tgtaatatgc cccttttgaa
     1921 agtgcacgtc ttcgacctgg caatctgttg tcgcgagcag cagcctgccc ggactcaccc
     1981 tcagcgcggc tgaatcgtcg cctatcccca cctcaacgca tccgattggg ctcggaaact
     2041 gctcctcgat gagacgaaga gcgctcagtt cgtttatcat gcggattaaa tcccggaaac
     2101 ttgcttattt agctgaatat tacctgtaaa aaaatccggt cttacggcgg ggggccattc
     2161 aaagattaag tcagtgctgc acaaatcagg gtcgggttcc gtcataacga atcgttaatc
     2221 caatcctcgt ttttatattt tactttaatt ttaggatatt tagcattata tgtcaataaa
     2281 attcgggttt tcggaaactc atctatacag gagaacggat tttcgaaatc tttcatcacc
     2341 catcgattta tgattcaata ccatgctgcg gcatacattg tcgtatagtt tctcttgatt
     2401 tcacatataa ttgtcagaaa ttattcgtcg caagcataca aaagctctga ctcaaaaaat
     2461 gcataatcgg gttctgaata tgacaggcaa cgtcgacggt cttgaaaaga gggtttatcc
     2521 gcgtataagc aacctgaccc ttgtaatact cgctcttcta ctggtcaagc tggccgtgca
     2581 gctctatcta taccggatag gctttctaag cgtaagcgcg gacgaattct cgcgcggcat
     2641 cacggccgcg cgctggtctc tagacgggac gctttcgact accatacgca tgggagactg
     2701 gctgccgttc gagacctata tcaacggtct ctcgctcaag gtgtgggaca acgtcatctg
     2761 gactccccgc atcacagcct tcatcttttc ctgtttcctc ctcgtttatt tcataaagct
     2821 cgttcaacac ctgtttggtc tgctgccggt tacgcttctc gcgggccttc tactggtctt
     2881 taacccctgg ttcatctggc tgagcggcac accgatgctc gaagtctact acctggctcc
     2941 cttcgtagcc ggtctttatt acgtatctaa atggatatcc gggcgacgag gggcctatct
     3001 ggttatcggc ggccttctgt tttttctatc gaccggtttt cacagccaga gctggatact
     3061 cgtgaacgtg gtaaaccttt gcctctgctt cttcgcctgg agcatgctca ggcagaggga
     3121 ctacggaggg ctcgtcatgc tcgccgggtt cttcatgctc ggcaacctgt tcatcatcat
     3181 ctaccttccg agcgattatc ttgcgacggg gcagtggctt gccatgttcc acagacacgc
     3241 acagggtacg atagagtact acggaggata cgaaatcggg ctttttcaca agctgtcgta
     3301 ctatcccgag ctcgtcgtga aatcctcgaa gctggtctgg attttcttcc ctataggtct
     3361 ctatcggctt catggcgaac cgggaatccg gatcaaactc ttccccttcg ctgtcggctt
     3421 gggcgcgctc ttcttctact cggtcttcaa cgtgctatcc gtccccgcct cagccgcccc
     3481 cggacgttac tcgctcccct ttttcattct gttcgtcccg tacgcagccc tcggtatata
     3541 cgcattgatt gaaggaggcg gatacttcgc cgacgtcagg atacggcgcg tggcggcggc
     3601 aatactcgtg attttcatac tgggtctcaa cttcatgaag gcaatcgact tcgaggtaaa
     3661 aagcgcaaaa gctgccgtga gcatcggccg atacctgaaa gagagcatgg agaaagacgg
     3721 cgccgatccg aaggagaccg tcatggtgga gctatcctac tgggattacc tttacgtcga
     3781 gctcgccgcc aggcacttcg atcgcgtgag gtatgacagg aaggaatcga acgagtacac
     3841 aggcatacct tccgacctta tctcgatggg agacgacgac attctcaggt acatgaaaag
     3901 cggggatacg aaattaatcg ccgtcaagga cgaggcgata aaaaagagac tcgacagttt
     3961 ggccttcatg cacaagataa aggacctcga cgtctgggca gtttatgaca tcgatctgaa
     4021 acacgattaa taaacggatt tgctctctgg aaaccgctgc ccctatattc aagagaagca
     4081 tagcgcggtc tagataagat ttagcatcgg aacggggcgt ctccgcggat ttccggattc
     4141 cctcaggtca accctcacta ccgcgatgcg taactctccc ccgtacgctt tcccctcaac
     4201 cgggcaaggg aaagcctggg gacgtagctc tgaatcttgt ccatgtatat ataaatcact
     4261 ggcgttagat aaagtgtgat gagctgcgag aaaaggagac cgcctaccac ggccaggccc
     4321 agcgggcggc gcgactcggc ccccgcgccg tggccgagcg cgagcggcag tgcccccaga
     4381 agcgcggcca tagtagtcat cattatggga cggaaccgta tcaggcagcc ctggtatata
     4441 gcctcgacag ggctcttccc ctcgctcctc tgcgcatcga gcgcgaaatc tatctgcatg
     4501 atggcgttct tttttacgat acctatcagc atgatcaagc ccacgaacgc gtatacgttg
     4561 agctcgaccc cgaaaatcat cagcactatc agcgcgccga accccgccga gggcagaccc
     4621 gagaggatag tgatcggatg gatgaagctt tcgtag
//
LOCUS       DASSJH010000332         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_514080, whole genome shotgun sequence.
ACCESSION   DASSJH010000332 DASSJH010000000
VERSION     DASSJH010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514080"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFJ67_07310"
     CDS             <1..624
                     /locus_tag="VFJ67_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290232.1"
                     /translation="KGRDIGPFLPKYEPAIIATMPSLYQQLPRTRHGEVVDENGQRLD
                     LMDPDVWITLGWGLADPGQDKVLAWLLPDVSDPVRRREIALDHLRKSLIRGKEFEDAI
                     DTPAELPPGLELYLIAGDAILTGSGLRVDKSTGKFEVSQYSPGDGTVTRASALMDERV
                     GGYWTPVLVSPIKWSNVMFLFTDHLGLTKSPAFSDNVLFILLEAEKS"
     gene            754..1713
                     /locus_tag="VFJ67_07315"
     CDS             754..1713
                     /locus_tag="VFJ67_07315"
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HET7290233.1"
                     /translation="MRGNTSLRQLINNSKRAMLIGIGGGGDIVGTIPTADLLGMFGIQ
                     CDFGGLSWERSVIDPMPGPRTFDEVKNARKLNDIVWFANKDTVTSTGVRFAESGVAEV
                     LGKETLLIDINPGPRAVAEGILHAAGELDADLIIGIDVGGDLLAFGNEPGLMSPLADS
                     IMTAAFAVLEKRIPTVMGLFGYGSDGELTQEELERSMSILAREGGLLGSGGITREALS
                     LLEKLIAVVPTEASRAPALYAKGEFSDTSIRSGRRQVSLSMSSTVTYYFSPDVLYEKL
                     SVLSRAVSLAESLEEANDCLHALGIYTEYDLELDKFNKERGIA"
     gene            2105..2620
                     /locus_tag="VFJ67_07320"
     CDS             2105..2620
                     /locus_tag="VFJ67_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET7290234.1"
                     /translation="MKPNKILPVLSLFILIFAASSQAFASEDLLSVNKTVFIQIVIFI
                     AAIFILNTLVFKPFMELIDRRDKLTKGSIKEARELEEKVKQIILEYEARLNEARAQAQ
                     EERNKIVREAEAAAAGVVAKTREETGALLDDAKKKIESEAGVIKEKLKGDVQLLSKEI
                     ASKILGREAGA"
     gene            2617..>2870
                     /locus_tag="VFJ67_07325"
     CDS             2617..>2870
                     /locus_tag="VFJ67_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290235.1"
                     /translation="MTGYGIINLILAAASEHGAFNWRFVMEHVVNLLILLGVLVYFLK
                     TPVKNFLIERRGTIGHEIDVAQKTIGEAKSKFEEYSKKLQA"
BASE COUNT          704 a          746 c          807 g          613 t
ORIGIN      
        1 aaggggaggg acataggccc cttccttcct aaatacgagc ctgccataat agcgacgatg
       61 ccctcactct accagcagct cccgaggacg agacacggcg aggttgttga cgagaacggc
      121 caacggctcg acctcatgga ccctgacgtc tggataacac tcgggtgggg gcttgccgat
      181 cccggtcagg acaaggtgct cgcatggctc cttccggacg tttccgaccc tgtcagaagg
      241 cgtgaaatcg ctcttgatca tttaagaaaa tccctcatac gcggaaagga attcgaggac
      301 gctatagaca ccccagccga gctccctccg ggcctcgagc tttatctcat agccggagac
      361 gcgatactga cggggtccgg cctgagagtc gataagtcca cgggcaaatt cgaggtgtct
      421 cagtacagtc ccggtgacgg caccgttacc cgagcgagcg cgctgatgga cgagagggtc
      481 gggggatact ggacgccagt gctcgtatct ccgataaaat ggtcgaacgt gatgttcctc
      541 ttcaccgatc acctgggcct taccaaatcc ccggcattca gcgataacgt cctattcatt
      601 ctcctcgaag cggagaagtc ctgaacggcc acccggcctg tctataatta actttcctca
      661 gctcaacagg tgataattga aagttgcgtc ccttgcagta gattatttaa cgctgattcc
      721 agtattacat ttagtccaac ccggagggag cgattgcggg ggaacacgtc actcagacag
      781 ttaatcaaca attcaaagag ggccatgctc ataggtatag gcggcggggg agatatcgta
      841 ggcacgatac cgactgctga cctcctgggt atgttcggaa tccagtgtga tttcggagga
      901 ctctcgtggg agaggtccgt gatagacccc atgccgggtc ctcggacgtt cgacgaggtc
      961 aaaaacgcgc gtaagttgaa cgatatcgtc tggttcgcga ataaagacac cgttacgagc
     1021 accggggtga ggttcgccga atccggggtg gccgaagtcc tgggaaaaga gactctcctt
     1081 atagacataa atccgggacc cagggcggtc gccgaaggaa tccttcacgc ggcgggcgag
     1141 ctcgatgccg acctgataat aggtattgac gtcggagggg acctgctcgc tttcgggaac
     1201 gagcccggac tcatgagccc actcgcggat tccattatga cagccgcttt cgccgttctt
     1261 gaaaagagga taccgactgt aatggggctt ttcggctatg gcagcgacgg tgagcttaca
     1321 caggaagagc tcgaaaggtc gatgtctata ctcgcaaggg aaggggggct cctcggaagc
     1381 ggggggatca cgagagaggc tctctcgctc ctcgagaaac tgatcgcggt agtcccgacc
     1441 gaggcgagcc gcgcccccgc gttatatgcg aagggggagt tttcggatac gagcataagg
     1501 agcgggagga gacaggtcag cctctccatg tcttcgacgg ttacttatta cttctctccg
     1561 gacgtgcttt atgaaaaatt gtcggtcctc tcccgcgcgg tctctctggc ggagagcctg
     1621 gaggaggcca acgattgtct ccatgccctg ggtatttata ccgagtacga tctcgagctc
     1681 gataaattca ataaagaaag agggatagcc tgatcccgcg gctcccggca ggatcccgct
     1741 ctcaatatcc cggcgatttt tcttcaataa atcggtcagg cactgcgctg tccgtgagat
     1801 cggacgccgt caattccccg caggtttgtt tatacggcat gtaattgttt gacactcccg
     1861 ccatttcatg ttaaatactt tcgttttttc aatgttctgc ccttaaggaa ttaagcccgc
     1921 aaatgagtct ggaagaatcg ttaatcagcc cgttagaaaa gctctctgtc gcgcacgcta
     1981 acgcattttc gtatttttcc gcagcggcgg ggtttgtcgg cgttctatca cacaaaaatg
     2041 cggtcgccgg gtaccgcatg taggctggcg gatccacgga tcaggcaaag gagatatgaa
     2101 tataatgaaa ccgaataaaa ttttgcccgt cctctctctg ttcatattga ttttcgcggc
     2161 gtcatcccag gcattcgcct ccgaggatct cctgagcgtg aacaagaccg tattcataca
     2221 aatagttata ttcatcgctg caatattcat cctcaatacg ctcgtcttca agcctttcat
     2281 ggagctgata gacaggcggg acaagctcac gaaaggctct atcaaagagg ccagagagct
     2341 cgaggagaag gtcaagcaga taattctcga atacgaagcc aggctgaacg aggcgagggc
     2401 tcaggcccag gaggagcgga acaagatagt ccgcgaggcc gaagccgccg ctgccggggt
     2461 agtggccaaa acgagggagg agacgggagc gctcctcgat gacgcgaaaa agaagatcga
     2521 atcagaagcc ggggttataa aagagaagct aaagggcgac gtccagctct tatctaagga
     2581 aatcgcctcc aagatactgg gcagggaggc aggcgcatga ccggatacgg aataataaat
     2641 ctcatactcg ccgcggcatc cgagcacggc gcatttaact ggcggttcgt aatggaacat
     2701 gtcgtcaacc tcctcatact cctcggagtg ctcgtttact tcctcaagac cccggtaaag
     2761 aatttcctga tcgagaggcg cgggaccata ggtcacgaga tagacgtagc gcagaagact
     2821 ataggcgagg cgaaatccaa gttcgaagag tattcgaaga agcttcaggc
//
LOCUS       DASSJH010000333         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_839210, whole genome shotgun sequence.
ACCESSION   DASSJH010000333 DASSJH010000000
VERSION     DASSJH010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839210"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFJ67_07330"
     CDS             <1..657
                     /locus_tag="VFJ67_07330"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET7290236.1"
                     /translation="IDDPTKETFNGTHLMGYYPFDDQGVPGRKTILVENGVLKNFLLS
                     RTPVKGFQNSNGHGRASYGLSPMARMSNTIIESSREYPREELKAQLIEEVKRQNKPFG
                     LIIKSMQGGETNTSSFNFQAFRGTPLVLYKVDPETGEETPVRDIEIVGTPIVTVNKII
                     ATGDDYAVFNGFCGAESGYIPVSTVAPSILVSEMEFQRESSKKEKPPLLPPPAFDTAK
                     "
     gene            859..>2302
                     /gene="acs"
                     /locus_tag="VFJ67_07335"
     CDS             859..>2302
                     /gene="acs"
                     /locus_tag="VFJ67_07335"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET7290237.1"
                     /translation="MSDVKKTIEDLLTEGRTFAPGKKLRKNANVKNESVYKKAARDRE
                     KFWANFAKELDWFKKWSSVLKWKVPHSKWFVGGKLNVSYNCLDRHINTARKNKAAIIW
                     EAESGESVTLTYWELYREVNKFANVLKSLGVEKGDRVTIYLPMIPELAIAMLACTRIG
                     APHSIVFGGFSAESLMDRINDSKARVLITSDGGYRRGGIVPLKKNADDALTDTPTIEK
                     VVVVKRVGDKAEVKMKKGRDFWWHELMEKAHSYCEPERMDSEDMLFMLYTSGTTGKPK
                     GIVHTTGGYLVGVYATTKWVFDLKEEDTFWCTADIGWVTGHSYIVYGPLANGATSLMY
                     EGAPDYPDIGRFWAIVEKYRVSIFYTAPTAIRAFMRWGEEYPKKYDLRSLRLLGSVGE
                     PINPEAWMWYYTHIGNGKCPVVDTWWQTETGSILITPLPGITKLKPGSATRPFPGIQA
                     DIVDENGKTIKEGAGYLVLRNPWPSMLRTIY"
BASE COUNT          600 a          605 c          660 g          437 t
ORIGIN      
        1 atagacgacc ccacgaagga aaccttcaac gggactcacc tcatgggtta ttacccgttc
       61 gacgatcagg gcgtgcccgg tcggaagacg atcctcgtcg agaacggcgt gctcaaaaac
      121 ttccttttat ccaggacgcc ggttaaaggg tttcagaatt cgaacggcca cgggagggcc
      181 tcctacgggc tctcgcccat ggcgaggatg agcaatacga taatagagtc ctcgagggaa
      241 tatccgaggg aggagcttaa agcgcagctg atcgaagaag tcaagcggca gaacaaaccc
      301 ttcggcctga ttataaaaag catgcagggg ggagagacca atacttcctc ttttaacttt
      361 caggccttca ggggcacgcc tctcgtgctt tataaggtcg atcctgaaac aggcgaggag
      421 acccccgtga gggacatcga gatcgtcggt acgcctatcg ttacggtgaa caagataata
      481 gcgaccgggg acgactacgc tgtattcaac ggtttctgcg gggccgaatc gggctacatc
      541 cccgtctcca cggtcgcgcc cagcattctc gtctcggaga tggagttcca gcgcgagtcc
      601 tccaagaaag aaaagccgcc cctcctcccg cctcccgctt tcgacactgc gaagtgagcg
      661 aaaactttcg atccgttctc cgcctgagcc ccgggccctg attcttatcg cgctcatagt
      721 gaaggacatt aagagagtga agccggggaa ttaagtgcct ccccacgacg caagatataa
      781 aatactgttc aaatcccctc agaattttgt tagtattgag tatcttactc catccgcaat
      841 tcttataggg gggtctatat gtcggacgta aagaaaacta tagaggattt gcttaccgaa
      901 ggccgcacct tcgcgcccgg caagaagctg agaaagaacg cgaacgtgaa gaacgaatct
      961 gtctataaaa aggccgcccg cgacagggag aaattctggg cgaatttcgc gaaagagctc
     1021 gactggttca aaaaatggag cagcgtgctc aagtggaaag ttcctcattc caaatggttt
     1081 gtcggcggca aactgaacgt ctcttacaac tgtctcgaca ggcatataaa tacggccagg
     1141 aagaacaagg ccgctattat atgggaagcg gaatcgggag agagcgtcac gctcacgtat
     1201 tgggagctct acagggaagt taacaagttc gcaaacgtgc tgaagtctct aggcgtggaa
     1261 aaaggcgaca gggtaacgat atacctcccg atgatccccg agctcgcgat cgccatgctc
     1321 gcatgcacca ggatcggcgc ccctcacagc atcgtattcg gagggttcag cgccgagagc
     1381 ctcatggaca ggataaacga ttctaaggcg agggttctta ttacatccga cggcgggtac
     1441 agaaggggag ggatagtgcc gctcaagaag aacgcagacg acgcgcttac cgatacgccc
     1501 acaatagaaa aggtcgtcgt cgtgaaaagg gtcggcgata aagccgaagt caagatgaaa
     1561 aaagggcggg acttctggtg gcacgagcta atggagaagg cgcactctta ctgcgagccc
     1621 gagaggatgg acagtgagga tatgctcttc atgctctata cgagcgggac taccggaaaa
     1681 cccaagggga tcgtgcacac aacgggaggc tacctcgtgg gcgtttacgc caccaccaaa
     1741 tgggtcttcg atctcaagga ggaggacaca ttctggtgca cggccgacat aggctgggtg
     1801 acagggcaca gctacatcgt ttacggtccc ctggccaacg gcgcgacctc gctcatgtac
     1861 gagggcgccc ccgactatcc cgacatcgga aggttctggg ccatagtcga gaagtaccgg
     1921 gtgagtatct tctacacggc gccgacggcg ataagggcat tcatgaggtg gggcgaggaa
     1981 tacccgaaaa aatacgacct ccgctctctc aggctcctcg gctcggtagg cgagccgata
     2041 aaccccgagg cctggatgtg gtactacacc catatcggca acggcaagtg cccggtcgtc
     2101 gatacgtggt ggcagacgga gacgggttcg atactgatta cgccccttcc gggtatcacg
     2161 aaactaaagc ccggctctgc aacgaggccg tttcccggca tacaggccga tatagtagac
     2221 gaaaacggga agacgataaa agagggggcg ggctatctcg tgctgaggaa cccctggccc
     2281 tcgatgctga ggaccatata cg
//
LOCUS       DASSJH010000334         5270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_81023, whole genome shotgun sequence.
ACCESSION   DASSJH010000334 DASSJH010000000
VERSION     DASSJH010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81023"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /locus_tag="VFJ67_07340"
     CDS             complement(<1..1657)
                     /locus_tag="VFJ67_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7290238.1"
                     /translation="MRVLWTTEKGYPVMGGAQITNLAFLRNLSEKYGHDCRIFCVYPR
                     KKRLNYGRVGLRTYRDTDELRSMVLGFKPDVIISSLEAGIHAQRIANLYNIPSVHYLH
                     SYEYAPPGRAERESWKVSQSREYSDESELKFIFDEADACVANSRFMKRRFERRYGRSF
                     RVIYPEFTVEDVTAGGTRAGGGKFITGICGFPYKGADIFYELARRIKHENFLLVGNSQ
                     YEYLKKFGKLGNVRVLPYTLAKKFLGMSKAVLVPSQWPEPFGRILVEAAANGIPVLAS
                     LTGGMKEIIAGTALGVRKFRDPGSWEKKLREILESEDARALNISEGKRVSGKFLKGRS
                     TTELDRLIKKLAAGKKPDYKAKSVVALCGRKKEKTAYSSVNSRWLAMLGEDGDYTTLD
                     LEVPSGPRRLPVDFFIHHDYQQDFGSVSVPDEGKFIAVRTWDFGRFPAKWVEKINECD
                     QQWVHSGWVRSNAVSSGIDRRRVKVIPHGIDETVFRPGGGAYGLSTDRSFKFLFVGAT
                     VFRKGVDVLLEAFGEAFNAGDDVCLVIKDNPRDVFYSNIKYKDK"
     gene            complement(1670..1942)
                     /locus_tag="VFJ67_07345"
     CDS             complement(1670..1942)
                     /locus_tag="VFJ67_07345"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HET7290239.1"
                     /translation="MKEDLNRVYAINNEVAHREIEGQILILRPDDNFLYTLNGSGRLI
                     WLGIAKRKKLSSIIKNVSKEFGIPDEAARSDVLAFVSELEAKGIIL"
     gene            complement(1959..2966)
                     /locus_tag="VFJ67_07350"
     CDS             complement(1959..2966)
                     /locus_tag="VFJ67_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290240.1"
                     /translation="MKNEYERIITLCAGGFPGTNRSLINLEKTTGLTRHFVLGGSQPG
                     WAEVKFYIELLKEKSPKRILFGGWSPVYDVFIKGIRSRSTEMGVYWTSSAGQVDISEE
                     AGLLDSIISSRRLKHKLFANGELARALSGSCDSIKHLPDTVLFPEEAVMKKREGKEDR
                     LVFSLFCSPFEYKRKNVLNTLLALSMLKRKYTLYLNGLSKNRFYGDILKKLRITYRDF
                     GWMTDDKYQAALGEVDLCLQISFAETFNYVAAEHIVKRIPVMTSRMVPAVDGMPAYIR
                     KRLVVDDPDSAFEIRDKIEYLTGEAGRLKYLGDRMHDVLKRENEKRIEQAKKVLKTII
                     E"
     gene            complement(2956..3963)
                     /locus_tag="VFJ67_07355"
     CDS             complement(2956..3963)
                     /locus_tag="VFJ67_07355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7290241.1"
                     /translation="MRRKKILIAAPLFLEWDLGSYIRSILTSKGIEVDTFAYQPFGSA
                     AEAERELIKKTKESKPDILLGLKLDRIGARVLKSVKKMGIFAALWYVDCVDGEVPEWI
                     KPLHREADIFFTTAGGMLDKYRRVSDTPSCWLYEGAHLDAFPLRAAGGNPKRIYRSEV
                     AFVGSIYYYDNCGEVVTKRERFLKRVSERFELKIWGPQGVRDARERWGTGYPAVEWPA
                     YNAELVKVCGGADIVLGTNTTNSVNCYFSNRTFITLASGGFHLTHYVPGLENMFENRK
                     HLVWYNTEEECFRLIDYYLRRPALRRKIAREGRDWARRRYGMRRQVSRMLRLIETHYE
                     K"
     gene            complement(3985..4992)
                     /locus_tag="VFJ67_07360"
     CDS             complement(3985..4992)
                     /locus_tag="VFJ67_07360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7290242.1"
                     /translation="MTNLRPIKADEKIAVAIPTKNRPSYLAVLIASLLNQTYTNFMIV
                     INDQSDSSVERDDAITDLTALARNTGHEVKFIRTEGGWKRHQQAMEAVPEQIDIILRI
                     DDDMLPDPRFLENILRPFRFFPDRPLAAVGGCNPEPNVKPVNLDVNLADSSWTSTLLD
                     PTWRLQGHLYYSDPAVLDVESLNGGAICYRRSAVEDAGGWAVEGYSDHAFREENDLCA
                     RLLERNYSLMVTTEALAWHLLAPGGGAREFIKTPRGNYIISDMSGIESDNKLFLERMK
                     LIAAKRGPAPSEPKRYRVSDLEKNLYKGSPLRTLKGSALKVIERSVLRKLRGLYWYFK
                     K"
     gene            complement(4989..>5270)
                     /locus_tag="VFJ67_07365"
     CDS             complement(4989..>5270)
                     /locus_tag="VFJ67_07365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7290243.1"
                     /translation="FPFVIGEGMGYGKPAILSADTGTSEILTHKHDGLVTETGSIESL
                     KEAILYMCDNSANIKTMGEAAYETASEYSWKRFEGSFCELISGLYDERR"
BASE COUNT         1084 a         1585 c         1246 g         1355 t
ORIGIN      
        1 ttttgtcctt atattttata ttcgaataga atacgtccct cgggttgtcc ttgatcacca
       61 agcatacgtc gtccccggcg ttaaacgcct cgccgaacgc ttcgaggaga acgtctacac
      121 ctttcctgaa cacggtcgca ccgacgaaca ggaacttgaa cgacctgtcc gttgacagac
      181 cgtacgctcc gcctccgggc ctgaataccg tctcgtctat accgtggggt atgaccttaa
      241 cccttctccg gtcgatcccg cttgaaacgg cgttactcct tacccatccg ctgtgtaccc
      301 attgctggtc gcactcgttt atcttctcga cccatttcgc gggaaacctc ccgaaatccc
      361 atgtcctcac ggcgatgaat ttcccctcgt ccggtacgga aacgcttccg aaatcctgct
      421 ggtaatcatg gtgtatgaag aaatcgacgg gcaggcgcct gggaccggac gggacttcga
      481 ggtcgagcgt ggtgtaatcc ccgtcctccc cgagcatggc gagccacctc gaattcacgg
      541 acgagtatgc ggttttttcc ttttttcgtc cgcaaagcgc gaccacgctc tttgccttat
      601 aatctggctt ctttcccgct gcgagctttt ttatgagcct gtcgagctca gttgtcgagc
      661 gtcccttgag gaactttcct gaaacccttt tcccttctga aatattcagc gctcttgcgt
      721 cctccgattc gagaatctct ctcagcttct tttcccagct ccccgggtcc ctgaatttcc
      781 tcacgccaag cgccgtgccc gcgattatct ccttcattcc gcccgtgagg ctcgcgagca
      841 caggtatgcc gttcgcagca gcctccacga ggatgcgtcc gaagggctcg ggccactgcg
      901 aagggacgag caccgcttta ctcatcccga gaaacttctt cgccagcgta tacgggagaa
      961 cccttacatt cccgagcttc ccgaatttct tcagatactc gtactgagag ttcccgacga
     1021 gcaggaaatt ttcgtgcttg attctccttg cgagctcgta aaaaatatcg gcgcccttat
     1081 agggaaaccc gcagatgccg gtgatgaatt tccctccgcc tgcacgcgtg ccgccggccg
     1141 taacgtcttc aacggtgaat tccgggtaaa tgacgcggaa gctcctcccg tatctcctct
     1201 cgaacctcct tttcataaag cgggaattag caacgcaggc atcggcctcg tcaaagataa
     1261 acttcagttc gctctcgtcc gaatattccc tcgattgaga gaccttccac gattccctct
     1321 ccgccctccc cgggggagcg tattcgtagc tatgcaggta gtgcacgctc ggtatgttgt
     1381 aaaggttggc tatcctctgt gcgtggatgc ccgcttcaag ggaggagata ataacgtccg
     1441 gcttgaagcc gaggaccatg ctccgcagct catccgtgtc cctgtaagtg cggagtccga
     1501 cacggccgta attaagcctt ttcttccggg gatagacgca gaatatacgg cagtcgtgac
     1561 cgtatttttc agaaagattc ctgaggaacg cgagattcgt aatctgcgcc ccgcccatta
     1621 cggggtatcc tttttccgtt gtccagagaa ccctcatccc ttccctttct tataaaataa
     1681 tcccttttgc ttcaagctca cttacaaacg ccaatacatc agaccgcgcg gcttcatcgg
     1741 ggatcccgaa ttccttcgaa acgtttttaa tgatggacga cagcttcttt ctcttagcga
     1801 tcccgagcca gatcaatctg cccgagccgt tcagcgtgta gagaaaattg tcgtccggcc
     1861 tgaggataag aatctgcccc tctatctccc tgtgggcgac ttcattgttt atcgcgtaaa
     1921 cccggttcaa atcttctttc atattcccgc ccttagagct attcgataat ggttttgagg
     1981 accttcttcg cctgctctat tctcttttca ttctctctct tgagaacatc atgcatcctg
     2041 tccccgagat attttaatcg tccggcctct cccgtcagat attcgatctt gtcccttatc
     2101 tcaaaagcgc tgtcggggtc gtccacgaca agcctcttcc tgatatacgc gggcatgccg
     2161 tccacagccg gaaccatccg cgaggtcata accggaattc tctttactat gtgctcggcc
     2221 gcgacataat tgaacgtctc ggcaaaagag atctggaggc agaggtcaac ttctccgagc
     2281 gcagcctgat atttatcatc ggtcatccat ccgaaatccc tgtatgttat ccggagcttc
     2341 tttaagatat ccccgtaaaa cctgttcttg gacaggccgt tcaggtagag ggtgtacttc
     2401 cttttgagca tcgaaagagc gaggagcgta ttgaggacgt tctttctttt gtactcgaac
     2461 ggggagcaga acaggctgaa tacgagcctg tcttcttttc cctcacgctt tttcatcaca
     2521 gcttcctcgg gaaaaagtac cgtgtccggg agatgcttaa tgctatcgca gcttcctgac
     2581 aatgcccggg ccaattctcc gttcgcgaag agtttatgct ttagcctccg ggagctgatg
     2641 atggagtcta aaagaccggc ttcctccgaa atatccacct gcccggcgct gctcgtccag
     2701 taaacgccca tctccgtact ccggcttcgg atgcccttta tgaatacatc gtacacggga
     2761 gaccatcctc cgaaaagtat gcgtttcggg ctcttttcct tgagaagctc gatgtaaaat
     2821 ttcacctccg cccagcccgg ctgagaccct ccgaggacga aatgcctcgt gagacctgtc
     2881 gttttctcga gattgatcaa cgacctgttc gtgcctggga acccgcccgc gcagagagtg
     2941 attatacgtt catattcatt tttcataatg cgtctcgatg agccggagca tcctgctcac
     3001 ctgtctcctc atgccgtaac ggcgcctcgc ccagtcacgc ccctcgcgcg caatttttct
     3061 tctcagcgcg ggcctcctca gataataatc gatcagccgg aagcattctt cttccgtgtt
     3121 ataccagacg agatgtttcc ggttctcgaa catgttttcg agacccggca cgtagtgcgt
     3181 cagatggaac cctcccgatg cgagtgtaat aaaggtccgg ttggaaaaat aacaattgac
     3241 cgaattcgta gtgttcgtcc ccaatactat atcggccccc ccgcacacct tcaccagctc
     3301 ggcgttgtag gcgggccatt cgactgcagg gtatccggtc ccccacctct ccctcgcgtc
     3361 cctcactccc tggggacccc atatcttaag ctcgaaccgc tcgctcaccc gcttgaggaa
     3421 cctctccctc ttcgtgacga cctcgccgca gttgtcgtaa tagtatatgc tgcccacgaa
     3481 ggcgacttcg ctcctgtata tcctcttcgg atttccgccg gccgccctca gcgggaaggc
     3541 gtccaggtgg gccccctcgt aaagccagca tgagggcgtg tccgataccc gacggtactt
     3601 atccagcatc cccccggctg tcgtgaaaaa gatatccgct tctctgtgta gcggctttat
     3661 ccactcgggg acctcgccgt cgacacaatc gacataccag agcgcggcga atatgcccat
     3721 cttctttaca gacttcaaaa cccgtgcgcc tatcctgtcg agcttcagcc ccaatagaat
     3781 gtcaggtttc gattccttcg tttttttgat gagctctctt tccgcttccg cggcggagcc
     3841 gaaaggctgg taggcgaacg tgtccacttc gatacccttc gacgtgagga tggaacgaat
     3901 ataactcccg aggtcccatt cgaggaacaa gggggccgcg atcaggatct ttttacgacg
     3961 caaaagggcg cactccggga accgtcattt tttgaaatac cagtagagcc ctctcagttt
     4021 tcttaatacg cttctttcga tgaccttgag cgcgcttcct ttcaaagtcc ggagcggaga
     4081 gcccttgtat aaattctttt cgagatcgct gaccctgtaa cgcttcggct cactcggagc
     4141 gggccctctt ttagcggcga tgagcttcat tctctcaaga aagagcttgt tatccgactc
     4201 gattccgctc atgtcggata tgatatagtt gccccgcggg gtcttgataa actccctcgc
     4261 gcctcctccg ggggcgagga gatgccatgc aagggcctcc gtagtgacca tcagggagta
     4321 gtttctttcg aggagcctcg cgcaaaggtc gttttcctcc ctgaacgcgt ggtccgagta
     4381 cccctcgacc gcccatcccc ccgcgtcctc aacggcgctc cgccggtaac atatcgcccc
     4441 tccgttgaga ctctcgacat cgagcacagc cgggtcgctg taataaaggt ggccctgcag
     4501 tctccacgtc ggatccaaga gcgtcgaggt ccaggagctg tccgcaaggt tcacgtccag
     4561 gttcacaggc tttacgttgg gttccggatt gcatcctccc acggcagcga gaggcctgtc
     4621 ggggaagaaa cggaaaggcc tcagtatatt ttcaaggaaa cgcgggtcag gcagcatatc
     4681 gtcgtctatc ctcaggatga tatctatctg ctccgggacc gcttccatag cctgctgatg
     4741 cctcttccag ccgccttcgg tccgtatgaa cttcacttca tgcccggtgt tcctggcgag
     4801 cgcggtaaga tcggtaatcg cgtcgtcccg ctcgacggaa gaatcgctct ggtcgttaat
     4861 tacgatcata aagttcgtgt aagtttgatt aagcaaagag gcgatcaaaa cagcgaggta
     4921 ggaaggacgg tttttcgtcg gtatggcgac cgctattttt tcgtccgctt ttataggcct
     4981 taaattagtc atcttctttc atcatacagt cccgaaatca attcgcaaaa gctaccctcg
     5041 aacctcttcc agctgtattc agacgccgtt tcgtaagccg cctcgcccat ggttttaata
     5101 ttagcactgt tatcgcacat ataaagaatc gcttccttga ggctctcaat agagcctgtc
     5161 tccgtaacga gcccgtcatg cttatgtgtc agtatctccg atgtgcccgt atccgccgac
     5221 aggatcgcgg gcttgccgta tcccatcccc tcccctatta cgaaagggaa
//
LOCUS       DASSJH010000335         5439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1379458, whole genome shotgun sequence.
ACCESSION   DASSJH010000335 DASSJH010000000
VERSION     DASSJH010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379458"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..658)
                     /locus_tag="VFJ67_07370"
     CDS             complement(47..658)
                     /locus_tag="VFJ67_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HET7290244.1"
                     /translation="MKERIISRLRESADIKLRFAKESVAEVEEAASAIIKSLKAGGKI
                     LIFGNGGSAADSQHIAAEFVNRYSKERKALPALALTTDSSILTSIPNDGSFENVFSRQ
                     IEALGSKGDVAWGLTTSGKSGNVLKAFKTAKSMGLRTIAFTGQDKSGLEKSAECCVSV
                     PSKSTPRIQELHITLAHIVCELVEDALTAKKETAKREQQKAEP"
     gene            complement(661..2475)
                     /gene="mutL"
                     /locus_tag="VFJ67_07375"
     CDS             complement(661..2475)
                     /gene="mutL"
                     /locus_tag="VFJ67_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET7290245.1"
                     /translation="MGKIRILSDNLVSKIAAGEIVERPASVVKELIENSIDAGSGKIE
                     IELEAGGKRLIKVSDDGEGMTRDDSLMSIERHATSKIKDTADLFTVGTLGFRGEAIPS
                     IASVSRFRMTTKTHDDVIGTVISIEGGVVRSVEETGAPPGTSVEVRNLFYNTPARHKF
                     MRKTETELSNVLDIVERESLSRPGTSFELSSEGRTLLRLPARESYSERVSQIYPGVKL
                     SQVGAVGGGVRVSGLMSGPLDTRSTTQKLFTYVNGRSVRDRFLTRIIIDSYGRMLEKG
                     RFPQGVLFIEMPAQEVDVNVHPTKNEVRFSNARLVGDLIRESIQGMLGGAPWLGGRVT
                     YYGNAGELHQTARESGRGYGGGALERGEGNGVLNGSPVYNRAAASHTPHAPQAAQDPH
                     TYPGEESRADTGLFAGSGFYSSLKVIGQLGDLYIICASPGGMILVDQHAAHERINYER
                     LKKTHLGEGSVQAQDLLVPEVLELSPHESDVLSRYGIELSSLGLSIESFGDNTFIIRS
                     VPALLKNSDLGGLVRDIVSEIKESGEEKSLSEKIDTVISTMACHSSIRANYELNPEKM
                     KALLRELDRAEFPHSCPHGRPVARELSYTDIERMFKRT"
     gene            complement(2588..2746)
                     /locus_tag="VFJ67_07380"
     CDS             complement(2588..2746)
                     /locus_tag="VFJ67_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290246.1"
                     /translation="MLKEYLRILRKHSDDVFGVGARTTLSFYTILSIACVVIPLLFFL
                     ILIISRIF"
     gene            2978..4378
                     /locus_tag="VFJ67_07385"
     CDS             2978..4378
                     /locus_tag="VFJ67_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290247.1"
                     /translation="MHLYSGRNILRVVTNDAVLGILASILLTVLLLGVFAPRSHAVDR
                     ELESPKRVLVVKDYKWASGGMGRPAIMKEITIQNKGKYDYQNIAIEVDLYTKNDIPLG
                     SLRGTVHDVLPHGSEKTFYNITFGIMSAELQNTIARVAGADLIEKGTPAQAKDLIQVK
                     GYEWSGGQYGTEGVLKSITLVNKSSDNWKDIKIRVDFLGIPGAKVGIRGFTSRATIHD
                     ILPARSERTYNNINVGFRHPDARSVDISVMSAKQISEKELKIKMAKKEGKRAVKKKKK
                     KPAPGEATGAPGEEGGTVPESQKELSLSERYKQKLAQERGEAPPTPEPGTESVKEAMM
                     EGGAGETTAEGTGEEETIEGEEEYEYEEEVPLPEEDIVVEDVKWGGGVTGTIGKLSEI
                     TLQNLSDITYTKIQIQIEFFSFKEESPMFSNKTTIYDVLPANSKKTFKNVDAGYLNAI
                     PQEIRVQVLTAVPMGQ"
     gene            complement(4436..>5439)
                     /locus_tag="VFJ67_07390"
     CDS             complement(4436..>5439)
                     /locus_tag="VFJ67_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HET7290248.1"
                     /translation="NRFEKIEYEPSEEEISEGISPETVFYKDTTKSVITYNDSPDVGF
                     DAGINPYRGCEHGCVYCYARPTHEFLGLSLGLDFESRIFVKEDAPGLLRKELSSPKYI
                     PQTIAVSGNTDCYQPAERRFRITRGCLEVLSEFRNPAGIVTKNHLVTRDIDLLKGFAE
                     WNGIAVAVSITTLDPALKRVMEPRASEPLLRLRAIEELSQNGIPVIVMVAPVIPGLTD
                     HEIPNIIRSASDAGARSAGFIMLRLPYGVSDLFTSWLGRHFPDRKEKVLARIRSVRGG
                     RLNSSEFHDRMRGKGVFAEQARELFEVACRKAGFDGSRIELSTAHFRRPGGTQLDLF"
BASE COUNT         1207 a         1529 c         1538 g         1165 t
ORIGIN      
        1 tagttttcaa ggacgataga aaaaaaatcc gcgccgggaa ccccgctcag ggctcggcct
       61 tttgctgctc ccttttggcc gtctccttct tcgcggtaag ggcgtcctcg acgagctcgc
      121 atactatatg ggcgagcgtt atgtgtagct cctggatccg gggagtggat ttggaaggga
      181 cgctcacgca gcactcggcc gacttctcaa gaccgctctt gtcctgcccg gtgaaggcta
      241 tggtcctgag ccccatcgac tttgcagtct taaacgcctt cagcacgttt ccggactttc
      301 cgctagtggt cagaccccat gccacgtccc ccttgctccc gagggcctct atctgccgcg
      361 agaagacgtt ctcgaacgag ccgtcgttag gaatagacgt gagtatcgaa gaatccgtcg
      421 taagagcaag cgccggaagc gctttccttt ccttcgagta tctgttgacg aactcggcgg
      481 ctatatgctg ggagtcggcg gcgctgcccc cgtttccgaa tatcagtatc tttcctccgg
      541 ccttgaggga ctttattata gctgatgccg cctcttccac ctcggctacg gattccttgg
      601 cgaagcgcag ctttatgtcg gcgctttccc tgagtcttga aataatccgc tccttcatat
      661 ctacgtcctc ttgaacatcc tctctatatc cgtgtaggaa agctccctcg ccacgggcct
      721 gccgtgcggg caagagtgcg ggaattctgc acggtcgagc tcccgcagca gcgccttcat
      781 cttctcggga ttgagctcgt aattggccct gatcgacgag tggcacgcca tggtcgaaat
      841 cacggtatct atcttttcac tgaggctctt ctcctccccg gactctttta tctcggatac
      901 tatgtccctc acgagacccc cgaggtctga atttttgaga agggcaggca cgctcctaat
      961 tataaacgta ttatcgccga agctctctat cgagagtccg agcgaggaga gctcgatacc
     1021 gtacctggaa aggacgtccg attcgtgggg ggagagctcg agcacctcgg gcacgagcag
     1081 gtcctgggcc tggacggaac cctcccccaa gtgcgtcttc ttaagccttt cgtaatttat
     1141 acgctcatgg gccgcgtgct ggtcgacgag gatcatgcct ccgggggacg cgcaaattat
     1201 atagaggtcc cccagctgtc cgatgacctt gagcgaggaa tagaacccgg acccggcgaa
     1261 aagccccgta tccgcccggg attcttctcc gggatacgta tgcggatctt gtgccgcctg
     1321 aggggcatga ggggtgtgag acgcggcggc ccggttatat accggagatc cgtttaaaac
     1381 gccgttaccc tcgcctctct caagcgcgcc tccgccgtat cccctgccgc tctccctcgc
     1441 ggtctggtgt aattcccccg cattcccgta atacgtgacg cggccgccca gccagggagc
     1501 cccgccgagc atgccctgaa tggactctct tatgagatcg cctacgagtc tcgcattgct
     1561 gaacctgact tcgttcttcg tcggatggac gttcacgtcc acttcctgag ccggcatttc
     1621 gatgaataag accccttggg ggaacctccc cttctcgagc atcctgccgt aggaatcgat
     1681 aattatgcgc gtcaggaacc tgtctctcac gcttctcccg ttcacgtatg taaatagctt
     1741 ctgtgtcgtg gacctcgtat cgagggggcc cgacatgagc cctgaaacgc ggacgccacc
     1801 gccaacggcc ccgacctggc tgagcttcac ccccggatat atctgggaga ccctctcgga
     1861 gtaagattcc ctcgcgggaa ggcggagcag tgtcctgccc tcgctcgaga gctcgaacga
     1921 cgtgccgggc ctcgaaaggg attccctctc gacgatatcg agcacgttcg agagctcggt
     1981 ctcggtcttc ctcatgaact tgtgtctcgc gggcgtgttg tagaagaggt tcctcacctc
     2041 gacgcttgta cccggaggcg ctcccgtttc ctcgacgctc ctcacgactc cgccctcgat
     2101 agagatcacg gtgcctatca catcgtcgtg agtcttggtc gtcatcctga acctcgacac
     2161 gctcgctatg ctcggtatgg cttctcccct gaagccgagc gttcctacgg taaagaggtc
     2221 tgccgtatct tttattttgc tcgtcgcgtg gcgctcgatc gacatgaggg aatcgtctct
     2281 cgtcataccc tcgccgtcat ctgacacctt tatgagcctc ttgccgcccg cctcgagctc
     2341 tatttctatc tttccgcttc cggcatcgat cgaattttca atcagttctt tcacaactga
     2401 ggccggcctc tccacaattt ccccagccgc tatcttggag acaagattat cggacagaat
     2461 ccttatctta cccatctcgt acgagccagt gtacataaaa gaagaatatg agtcaaagaa
     2521 gaggggccct ttctcgggag agcgatcggg actgactccg tgcatgcgga atcatcacgg
     2581 agacacgtca aaatatcctg gatatgatga gtatgaggaa gaaaaggagc gggataacga
     2641 cgcacgcgat cgatagaatc gtgtagaagg agagcgtcgt ccgggcgcct acgccgaaga
     2701 cgtcgtccga gtgtttcctg agaatccgta gatattcctt gagcatttgg gttatatata
     2761 atttactcga aaagcattaa gattataacc gggtcgaaac cgggagggac agacggggga
     2821 agcgggcaag agacgcgccc gagcggtcta tacacgcttt atccggctgg aaaatccata
     2881 tagacgcccg gagaactcat taaatagtaa tgctacaatt tcgaatccga ttatgtataa
     2941 tcatatccta ttctcgcagt caaactaagg agatacgatg catctttatt ccggacgcaa
     3001 catcctcagg gtcgtgacta acgacgcagt cctcggcata ttagcttcga tacttctaac
     3061 agtcttgcta ttgggcgtat tcgctccccg ctcccatgca gttgacaggg agctggaatc
     3121 gccgaagagg gtgctcgtcg taaaagacta caaatgggct tcgggcggca tgggcaggcc
     3181 agcgatcatg aaggagatca ctatacagaa caagggcaag tacgactacc agaacatagc
     3241 gatcgaggtc gacctctaca cgaagaacga catccctctc ggctcgctga ggggaaccgt
     3301 gcacgacgtg cttccccacg gcagcgagaa gacattttac aacatcacct tcggcattat
     3361 gagcgccgag ctccagaaca cgatcgcgcg cgtggcgggg gccgacctca tagagaaagg
     3421 cacgcccgcc caggccaagg acctcataca ggtgaaagga tatgaatgga gcgggggaca
     3481 gtacggcact gaaggggtgc tcaaaagcat cacgctcgtc aacaagagca gcgacaactg
     3541 gaaggacata aagataaggg tcgattttct cggcataccc ggagccaagg tggggatcag
     3601 ggggttcacg agcagggcga ccatacacga catactcccc gcaaggagcg agcgcactta
     3661 taacaacatc aacgtcggtt tcagacaccc ggacgcgagg agcgtcgaca taagcgtcat
     3721 gagcgcgaag cagatctcgg agaaagagct caagataaag atggcaaaga aagaggggaa
     3781 gcgggcggtg aagaagaaaa agaagaaacc cgcgccgggc gaggcgaccg gggctcccgg
     3841 cgaagaggga ggcacagtgc ccgagagcca gaaggagctc tcgctctcag agagatataa
     3901 gcagaagctc gctcaggaga gaggagaagc tccgccgacc cccgagccgg ggaccgagtc
     3961 cgtaaaagaa gccatgatgg aaggcggcgc cggagagact acggccgagg ggacggggga
     4021 agaggaaacc atcgagggcg aagaggaata cgaatacgag gaagaagtcc ccctgccaga
     4081 ggaggacata gtagtcgagg acgtcaagtg gggcggagga gttacgggga ccataggaaa
     4141 gctatcggaa attacgctcc agaacctgag cgatataacg tacaccaaga tacagatcca
     4201 gatagagttc ttctctttca aggaagagtc gcctatgttc tcgaacaaga ccaccatata
     4261 cgacgtgctg cccgccaaca gcaagaaaac tttcaagaac gtggacgcag gctatctgaa
     4321 cgcgataccg caggagatac gcgtacaggt gctcaccgcc gtgccgatgg gacagtagga
     4381 gcgactgtgt tctccgtaat agcccggagc gcgggcttaa gtatatttgt gtgaattaga
     4441 agaggtcgag ctgcgtcccg cccggccttc tgaagtgggc ggtcgagagc tcgatcctcg
     4501 agccgtcgaa tcccgccttc ctgcaggcga cctcgaacaa ttccctcgcc tgctcggcaa
     4561 atacgccctt ccccctcatc ctgtcgtgga actcggagct gttgagcctg cctccgcgta
     4621 cggaccttat tctggcaagg actttttctt tcctgtcggg gaaatgcctc ccgagccatg
     4681 aggtaaacag atcggatacg ccgtagggga gcctgagcat aatgaaaccc gcgctcctcg
     4741 cgccggcatc ggacgcgctc ctgatgatgt tcggtatttc gtggtcggtg agtccgggta
     4801 tgaccggggc gaccatgact atgacgggga tgccgttctg tgaaagctcc tctatcgcac
     4861 ggagtctgag cagaggctct gatgcgcggg gctccatcac cctttttagc gccgggtcga
     4921 gcgtcgtgat agagacggcg accgctatcc cgttccattc cgcaaagcct ttaaggaggt
     4981 cgatgtcgcg cgtgacgagg tggttcttcg tgacgatgcc ggcggggttc ctgaattctg
     5041 aaagcacttc gaggcagccg cgcgtgatgc ggaagcgccg ctcggccggc tgatagcagt
     5101 ccgtattgcc gcttactgct attgtctgtg gaatgtattt cggcgaggag agctcttttc
     5161 tcaggagccc gggagcgtct tccttgacga aaatcctgga ttcgaagtcg agcccgagcg
     5221 agaggcccag gaactcgtga gtgggccggg cgtagcagta gacgcagccg tgctcgcatc
     5281 ccctgtaggg gtttatcccg gcgtcgaagc cgacgtcagg gctgtcgttg tatgtaataa
     5341 ccgacttggt cgtgtcctta tagaagactg tttcggggga tatgccttcc gagatttcct
     5401 cttccgaggg ctcgtactcg atcttttcga atctgttag
//
LOCUS       DASSJH010000336         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_298127, whole genome shotgun sequence.
ACCESSION   DASSJH010000336 DASSJH010000000
VERSION     DASSJH010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298127"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..>2031)
                     /locus_tag="VFJ67_07395"
     CDS             complement(164..>2031)
                     /locus_tag="VFJ67_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7290249.1"
                     /translation="GRSTMIGIRQKLALGFGGLLAIVVVIGLLTMSRIRELGYAVDVI
                     LKENYLSVVACQDMKETLERMDSGILFTFAGNNEEGLKLIDENTGKFRSALKSELNNI
                     TLPLEGEKAREIEKLFGEYTKSIPLVTGASRPFQERNEAYFSTLLPLFQKIKSLAQEI
                     LIMNQKSMSDANDEARALAASTYRQMLTAIIASAVIAILFGYLTHKWVLRPINRLIES
                     TGEIRRGNLDVVIESGSHDEIGKLSESFNEMTAALRQVRKSERMNLIRLRRAMEEVVK
                     ALPDAIAILDMEGRVEVSTDTAQRMFGLKPGALVNDLGYEWMPGLIGRALVEDRMVDL
                     DSKNGYVQRFAEGREYFFKPIAVPIPVKPKSGEHTGSAIILSDVTQLHEQAELKRDVV
                     STVSHQLRTPLTSLRMSIHLLLDERVGDLNEKQAELLMAAREESERLVNILTDLLDLN
                     KMESGKTNLDIGNYSPHTLAREGIEPFHTEAKDKGVTVINGVPEDLPDVPADLFRIRH
                     VFANLISNSLRFTKPGGVIIVRAEREDDSIRFSVEDTGEGIPPEHLSRIFDQFYRVPG
                     QDEKSGVGLGLAIVKEIVEAHGGSVGVKSEAGRGSSFEFTLPLERHSTEIRIHHA"
BASE COUNT          420 a          601 c          512 g          498 t
ORIGIN      
        1 cgtaaaccag ataaaaaaag cagaaaacca gaacgatcga cgctatccac agcatttggg
       61 cctccctata ttttttccaa aaagaccgtc attatcccta gcaggataaa agctatcaaa
      121 ccaagagcca gcattataaa catcagcatt ttcttatgcc tcctcaagcg tggtgaatgc
      181 gtatttctgt ggaatgtcgt tccagcggca gcgtaaactc gaacgaggat cccctcccgg
      241 cctcgctttt cacgcccacc gatccgccgt gagcttccac tatctccttc actatggcga
      301 gaccgaggcc tacccccgac ttctcgtcct gccccgggac gcggtagaac tgatcgaata
      361 tcctcgacag gtgttcgggc gggataccct caccggtatc ctcgacagag aaacggattg
      421 aatcatcttc acgctcagcc ctgactatga ttacgccgcc cggctttgtg aacctcagcg
      481 agttggaaat aagatttgca aacacatggc ggatcctgaa gagatcagcc ggtacatcgg
      541 ggaggtcttc aggaaccccg tttatgaccg tgacgccctt gtcctttgcc tcggtgtgga
      601 agggttctat accctccctc gcgagagtat gcggagaata attcccgata tcgaggtttg
      661 tcttccccga ctccattttg ttcaaatcga gaaggtcggt cagaatgttc acgagccttt
      721 cgctttcttc tctcgccgcc atgaggagct ctgcctgctt ctcattgaga tcccctaccc
      781 tctcatccag aagcaggtgt atcgacatgc ggagtgacgt gagcggcgtc ctcaactgat
      841 gggacaccgt tgagacgaca tcccgcttga gctctgcctg ctcgtggagc tgtgtcacgt
      901 cgctcagtat tattgcggaa cccgtatgct cgcctgactt gggcttgacc ggaatgggca
      961 cagcgatggg cttgaagaaa tactcgcggc cttcggcgaa gcgctgtaca tatccgttct
     1021 tcgaatccag gtccaccatc ctgtcctcga cgagcgccct gccgatgaga ccgggcatcc
     1081 attcgtaccc gaggtcattt acgagcgcgc cgggcttgag accgaacatg cgctgagccg
     1141 tatccgtcga cacctccacg cgcccctcca tgtcgagaat ggctatcgcg tcaggcaggg
     1201 ccttcacgac ctcctccatc gccctccgca agcggatgag gttcatcctc tcgctcttcc
     1261 tcacctgcct gagagccgcg gtcatctcgt tgaacgattc cgagagcttc cctatctcgt
     1321 cgtgcgagcc tgactcgata acaacatcga ggttcccgcg gcgtatctct ccggtcgatt
     1381 ctataagcct gtttatcggc ctcaataccc acttgtgcgt gagataaccg aagaggatcg
     1441 ctatcacggc tgacgcgatg atagccgtaa gcatctgcct gtatgtggac gcggcgagcg
     1501 ccctcgcttc gtcgttcgcg tcgctcatgc tcttctggtt catgatgagt atttcctgtg
     1561 cgagcgattt tattttctgg aacagtggca acagtgtcga gaagtaagcc tcgttccgct
     1621 cttgaaacgg gcgtgaagcg cctgtgacga gcggaatgct cttggtatat tctccgaaga
     1681 gtttctctat ttcacgggct ttttccccct ccagcggaag cgttatattg ttgagctcgg
     1741 acttgagagc cgacctgaac ttacccgtgt tctcgtcgat gagtttcagc ccctcttcat
     1801 tgttgcccgc aaacgtaaag aggattccgc tgtccatgcg ctcgagggtc tccttcatgt
     1861 cctgacaggc gacgacgctg agatagtttt ccttgaggat cacgtcgacc gcgtagccga
     1921 gctccctgat gcgcgacatg gtgaggaggc cgatgaccac gactatcgcg agcagaccgc
     1981 cgaacccgag ggctaatttt tgacgtatgc ctatcattgt gcttcttccg t
//
LOCUS       DASSJH010000337         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_82106, whole genome shotgun sequence.
ACCESSION   DASSJH010000337 DASSJH010000000
VERSION     DASSJH010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82106"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFJ67_07400"
     CDS             <1..321
                     /locus_tag="VFJ67_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HET7290250.1"
                     /translation="AFTPILIKYALCLLERIYKEGYEYKKAGVALMDIVPANESQLNL
                     FVKLDHSNHESLMRAMDRINSQWGRETLRSGASGYKRPWGMKRAMLSQRFTTRWEELL
                     RIGD"
     gene            460..1527
                     /locus_tag="VFJ67_07405"
     CDS             460..1527
                     /locus_tag="VFJ67_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7290251.1"
                     /translation="MSVRSRFNIHPLLAGASIVIILAGVREIAPVLNIFLVALLLAVA
                     VSPLLTWQLRRGWPKAASLAVTILIVIVIMGSVTTILGVAINNIIGKAPFYQDKIAEL
                     YHAGLNALTARGIDVSDVQQLNIFSPDKIVGYMTSFLQNIFSTFGNFFFVVLLMVFIL
                     IEFADIAIKSGRGEFAEDSWQSRFGDISDDLKKYVTINAVAGLVAALADFALLLVLGI
                     DFAVLWGFLAFLFSFIPNIGFILSVVPPALLALLEFGWGRCILVIVGYVIFNSIIDNV
                     IKPKFLGKEFKMSILVVFLSLLFWGWLLGAIGAILGVPLTMAVKRIVDFASKDMEADG
                     RAAEEPVIPLPETKPEKSAET"
     gene            1960..>2565
                     /locus_tag="VFJ67_07410"
     CDS             1960..>2565
                     /locus_tag="VFJ67_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7290252.1"
                     /translation="MPGIGAIIQTICLLLLLVTLAFPSRADEAKEIGGTLFISKGCMG
                     CHGVSGRGGVGPALAHTALTFDAFIGQIRHPRNIMPAFPAQAVSDENAREIYVFLKDV
                     PPPAERLQKDIPHGVLDPGTCAPCHKTLHPEIVSQFESSAMGTEGMQNPRVDFPLKQL
                     TCANCHGTDHDVIMASKGRVPETMCAACHAQIYKEHVLDAGH"
BASE COUNT          579 a          753 c          667 g          566 t
ORIGIN      
        1 gcctttaccc ctattctcat caaatatgcc ctttgccttc tcgaaagaat ttacaaagaa
       61 ggatacgaat acaaaaaagc gggcgtggcc cttatggaca tagtcccggc gaacgaaagc
      121 cagcttaacc tgttcgttaa gctcgatcac tcgaatcatg agagcctgat gcgcgcgatg
      181 gacaggataa actcgcagtg gggcagagaa accctcaggt cgggggcgtc cggttataag
      241 agaccatggg ggatgaaaag ggcaatgctg tcgcagcggt ttactaccag atgggaagag
      301 cttttgagga tcggagatta agtgatgtca aaggtggaaa atactatttt tcttgctcat
      361 tcatctccct acaccccttg ataaaaactt cccccctcta gtggtataat tctaccggac
      421 agaggcgcgg cgaggagccg ccttccggcc gtaatccgaa tgagcgtgag atcaaggttc
      481 aacatacacc cgctcctggc gggcgcgtct atcgtcatca tactggcagg ggtgcgcgag
      541 atcgcccccg tgctcaacat attcctcgtc gcgctgcttc tcgccgtggc ggtctccccg
      601 ctccttacgt ggcagctccg gaggggctgg cccaaggccg cttccctcgc cgtcacaata
      661 cttatagtca tagtcatcat ggggtccgtg accaccattc tcggcgtcgc gataaacaac
      721 atcattggca aggccccttt ctatcaggac aagatagccg agctttacca cgcgggactg
      781 aacgccctca cggcgagagg gatagacgtg agcgacgtcc agcagctcaa catattcagc
      841 cccgacaaga tagtgggcta tatgacttcg ttcctccaga acatattctc tacgttcggc
      901 aacttcttct tcgtcgtgct cctcatggtg ttcatactca tcgagttcgc cgatatcgcg
      961 ataaagtccg gcaggggcga gttcgcggag gactcgtggc agagcaggtt cggcgacata
     1021 tcggacgacc tcaagaagta cgtcacgata aacgctgtag ccgggctcgt tgccgcgctc
     1081 gcggacttcg cgctcctcct ggtgctgggc atcgatttcg cggtgctctg ggggttcctc
     1141 gcattcctct tcagcttcat accgaacatc gggttcatac tctcggtcgt cccgccggcg
     1201 ctcctcgccc tgcttgagtt cgggtggggg cgctgcatcc tcgtcatcgt gggctacgtg
     1261 atcttcaact ccattatcga caacgtcata aagccgaagt tcctcggcaa ggaattcaag
     1321 atgtcgatac tcgtcgtatt cctgtcgctg cttttctggg gatggctgct cggcgcgata
     1381 ggggcgatac tcggcgtccc gctcaccatg gccgtcaagc gcatagtcga tttcgcaagc
     1441 aaggacatgg aggcggacgg gcgcgccgcg gaggagcccg tcattcctct gcccgaaacg
     1501 aaacctgaaa agagcgcgga gacctgaccg cgggaatatc atattttgtc atcctgaact
     1561 acgtttctga gcgtgcgcaa gaaacggacg ccattggctg aatccaataa tgcaactatt
     1621 ctccgcttat tcgtttatta agccaaaata ttcaggatct cttttttgtc cttttcatca
     1681 ttaccgaata gcacaagcca aaataaatcg ggaatccagt ctttattaaa tcgtaggagc
     1741 ggcttccagc cgcgatgcct tatcatgtca ttgcgagcgc agcgcggcaa tctagcctta
     1801 aaaccccttc ttcccgatcg cacatgcggt gtgcgaccaa aggacgcata tacaacactt
     1861 cctttcagtt ccctccttcc acattgaaag ttcgccgtgt tgcagatgca atcgaaatat
     1921 atcttccaat ccgcaaaata ttttgttatg ataggacgca tgccgggaat aggggcgatc
     1981 atacaaacaa tctgccttct cctcctcctg gtaacgctcg cgttcccctc acgggccgat
     2041 gaagctaaag agatcggcgg cacgcttttc atatctaaag gctgcatggg ctgccacggc
     2101 gtgagcggcc ggggcggcgt agggcccgcg ctcgcgcata ccgcccttac attcgatgcg
     2161 ttcataggcc aaatccgcca tccgaggaac ataatgcccg cgttccccgc ccaggccgtc
     2221 agcgacgaaa acgcgcgcga aatctacgtg ttccttaaag acgtcccccc tcccgcggag
     2281 aggctgcaaa aagacatacc gcacggcgtg ctcgaccccg ggacctgcgc cccctgccac
     2341 aagactcttc atcccgagat agtgagccag ttcgagtcga gcgcgatggg gacggaaggc
     2401 atgcagaacc ctcgcgtcga ttttcccctc aagcagctga cgtgcgccaa ctgccacgga
     2461 accgaccacg acgtcatcat ggcgtccaaa ggacgggttc cggaaaccat gtgcgccgcg
     2521 tgccacgcgc agatatataa agagcacgtc ctcgacgcgg ggcat
//
LOCUS       DASSJH010000338         5267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_190941, whole genome shotgun sequence.
ACCESSION   DASSJH010000338 DASSJH010000000
VERSION     DASSJH010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190941"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFJ67_07415"
     CDS             <1..321
                     /locus_tag="VFJ67_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET7290253.1"
                     /translation="MQLLEVIPATLKVLSKAGLSLADMDVIEINEAFASVVLAWASEL
                     KPDMGKVNPNGGAIAHGHPLGATGAALMTKLLFELERADGQFGLQVMCIGHGMATATV
                     IERL"
     gene            350..1258
                     /locus_tag="VFJ67_07420"
     CDS             350..1258
                     /locus_tag="VFJ67_07420"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HET7290254.1"
                     /translation="MNDEIKRGTLCSLDSLEADEEMYGTATTIETWLALEYSGRWSGD
                     AFRDSRIPEAAKAGIKRYLEKFPNPRLQLVKKEKRSGDGIKLFIAHSGEKDPVFREIT
                     LGGYEELLDLDLESVTRNPATERGEPLFLICTNGEHDKCCGKFGVPVYRDAANGTHGG
                     NIWQTTHIGGHRFASTFVCLPHGLYYGRVREGARADEIFSEYKQGRIDLRSYRGRSCY
                     SPPVQAAEYFLRKEKGVREINAYSFKAAGEENGEIRAEFISATNDRIYIVRLRRLDTS
                     LRVLKSCGDKERSFIPQYILQSIVEG"
     gene            complement(1317..1829)
                     /locus_tag="VFJ67_07425"
     CDS             complement(1317..1829)
                     /locus_tag="VFJ67_07425"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7290255.1"
                     /translation="MEVFECVKTLSSIRSYIKKAVPDGIVREVIEAGRLAPSAHNDQP
                     WQFILVRDKAKLEGLEKYCISGRFVSEVDFAVAVVTDPSSKWHETDGTRAVQNMVLTA
                     WSHKLGSCWIGNIDRDGLKKYLNIPDKFHVLTVLPFGYFDQRQVSSAKNRKSPEQVFH
                     LDTFGNRAIK"
     gene            1988..2464
                     /locus_tag="VFJ67_07430"
     CDS             1988..2464
                     /locus_tag="VFJ67_07430"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7290256.1"
                     /translation="MPDPVKIGGRAPVFSLPSESGEIVRLDDYIGKKPLVLYFYPKDN
                     TVVCTKEACAFRDNYDEFRRLEGAEVFGISSDPVESHKSFASEHRLPFRLLSDEKGAV
                     TELYGVPRTLGIFPGRVTYVIDVDGIVIRIISSQLGYKKHVEEALQALRSTQNEKI"
     gene            2593..3975
                     /gene="argH"
                     /locus_tag="VFJ67_07435"
     CDS             2593..3975
                     /gene="argH"
                     /locus_tag="VFJ67_07435"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET7290257.1"
                     /translation="MLKKPWGGRFKKDTHKIAEKFSASIDYDKRLYKEDIEGSVAHVT
                     MLAENGIILKSEAEKIIKGLRQIGKEIDSGKFPFREEFEDIHLNIEKRLIEKIGDTGG
                     KIHTARSRNDQIALDMRLYLRKEINEIILLIKDIAVSLIGLAEKNIDVVLPLYTHLQR
                     GQPVLLSHHLMGLYEMLKRDRERYIDCRARVNVMPLGAGAGAGTTFPIDRELVARLLD
                     FPKVTQNSIDTVSDRDFIVEFIAVSANLMMHLSRMSEEFVLWSTKEFDFIDLGDEFTT
                     GSSIMPQKRNPDMAELIRGKTGRVYGGLVTILTVMKGLPFSYNRDMQEDKEPMFDTAD
                     TVKSSLEVLRAMLGGITFKNENMKKALAAGFITATDIADYLTRKGMPFRKSHEVTGKI
                     VGYAEEKGRELMNLHITEYKMFSRLFEDDLFDHITLEGSVSGRKSFGGTARQNVVRMI
                     AQGKKETGKW"
     gene            3992..4411
                     /locus_tag="VFJ67_07440"
     CDS             3992..4411
                     /locus_tag="VFJ67_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7290258.1"
                     /translation="MTEAKYREFLLRGTKTGKLATVRKDGRPHVVPVWFELDGDAVIF
                     PTSDRSVKFMNMKHDPRVSITIDDQTPPYSYVMIEGTVSFSEDPGELLYWATRIGGRY
                     MGEDQAEAYGRRNSTPGEVIVRIIPSKVSAFADVAGW"
     gene            4624..>5267
                     /locus_tag="VFJ67_07445"
     CDS             4624..>5267
                     /locus_tag="VFJ67_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET7290259.1"
                     /translation="MKLHSERKPATHSNITRVMSRFLSAEKKYSPILFWSLIVGALTG
                     AAGAVFQLSIEKVQDSRELLLRIIPQSFVFQLAASVLFSAVLVYIAFLLVRRLAPEAS
                     GSGVHEIEGALDDVRPIRWKNVLPVKFLGGLCSLGGGMVLGREGPTIQMGGNIGKMVA
                     DIFRAGKEDTHALIAAGAGGGLTAAFNAPLAGILFVFEEMRPEFKYNFLSVQSV"
BASE COUNT         1390 a         1367 c         1436 g         1074 t
ORIGIN      
        1 atgcagctcc tcgaggtcat acctgccaca ttaaaagtac tctcgaaggc agggctctcg
       61 ctcgcggata tggacgttat agaaattaac gaggccttcg cctctgtggt actagcatgg
      121 gcaagcgagc tcaagcccga catgggaaaa gtgaatccga acggcggggc gattgcccac
      181 ggccatcccc tcggggcaac gggagcggcg ctcatgacaa agcttctatt cgagctcgag
      241 cgcgcggacg gacagttcgg gcttcaggta atgtgcatag ggcacggtat ggccaccgcg
      301 accgtgatcg aaagactctg accaacataa aaggacaggt gacctcaggt tgaatgacga
      361 gataaagcgg ggcactctat gctcgctcga ttcgctcgaa gcggacgaag agatgtacgg
      421 gaccgcaacg actatagaga cttggctcgc tctcgaatat agcgggcgct ggtcgggcga
      481 tgcgtttcgc gacagcagaa taccggaagc agcaaaggcc gggatcaaac gctatcttga
      541 aaaattcccc aatccacgtc tccagctggt aaaaaaagaa aaacgaagcg gggacggcat
      601 aaagctgttc attgctcatt caggggagaa ggacccggtc ttccgcgaaa tcactctcgg
      661 cggatacgaa gagctgcttg atctagattt agaatcggtc acaagaaacc ctgccacgga
      721 gcgcggcgag cctctttttc ttatatgcac aaacggcgag cacgacaagt gctgcggaaa
      781 gttcggcgtg cctgtatacc gtgacgccgc aaatggcaca cacgggggga acatctggca
      841 aacgactcac atcggaggcc acaggttcgc ctccacgttc gtatgtctcc cccacggcct
      901 ctattacggg cgcgtgaggg agggtgcgag ggccgacgag atatttagtg aatataaaca
      961 agggaggatc gacctccgaa gctacagggg acgctcctgc tacagccctc ccgtacaggc
     1021 ggccgagtat tttttgagaa aggaaaaagg tgtgagagaa ataaacgcat acagttttaa
     1081 agccgcaggc gaagagaacg gagaaattag ggcggaattt atctccgcaa caaacgatag
     1141 gatttatatt gtcaggttga gaaggctcga cacttctctc agagtgctca agagctgcgg
     1201 ggacaaggaa aggtcgttta tcccccaata catattacaa agcattgtag aaggatgaga
     1261 agcgaagccc cgttcgtccg tccgcaccta catatataac ggcaaccggg tccgccttat
     1321 ttaatggccc tgttcccgaa cgtatccaga tgaaacacct gctcgggtga ttttctattt
     1381 ttagccgagc tcacctgtct ctgatcgaaa tatccgaatg gtaaaacagt gaggacgtga
     1441 aacttgtccg gaatattcag atattttttt agcccgtccc tgtcgatatt cccgatccag
     1501 cacgatccga gcttatggct ccaggcggtt agcaccatat tctgaacggc gcgtgtaccg
     1561 tccgtctcgt gccacttcga ggacgggtcg gtcacgaccg cgaccgcgaa atccacctcg
     1621 gaaacgaacc tcccgcttat gcagtacttt tcgagccctt ccagctttgc cttgtccctc
     1681 acaagtatga actgccaggg ctggtcgttg tgcgcgcttg gcgcgagcct gccggcctct
     1741 atgacctctc tgacgatacc gtcaggtacc gccttcttta tgtagcttct tatggagctc
     1801 agggtcttta cacactcgaa cacttccatt ctattatcgc ctccggcttt cgaagttaca
     1861 gtgatgcata tatatccaga tattatagag tatcttattt cgtacaagga ttcccggtct
     1921 cgcttgttac ggactggacc gggaatcatt cggtatcttt gtatataact ctataaatgg
     1981 aaaagaaatg ccggacccgg tcaaaatcgg cggcagagcg cccgttttca gcctcccttc
     2041 ggagagcggc gagattgtaa ggctcgacga ttacataggg aagaaaccct tagtcctcta
     2101 tttctatccg aaggacaata cggtagtgtg cacgaaggag gcgtgcgcgt tcagggacaa
     2161 ttacgacgag ttcaggcggc tggaaggcgc ggaggtattc ggtatcagct cggaccctgt
     2221 cgaatcccat aaaagcttcg cttcggaaca cagactcccc ttcaggctct taagcgatga
     2281 gaaaggggca gtgacggagc tctacggcgt ccccaggacc ctcggcatat tccccgggag
     2341 ggttacctac gtaatagacg tggacggaat agttatacgc ataatctcct cccagctcgg
     2401 ctataaaaag cacgtcgaag aagcgcttca agccctgaga tccactcaaa acgagaaaat
     2461 atagcgtatc cggggcatgt ccccgcccct gagccttata tattgtccgc agccgtgata
     2521 tagtttctcc ggcgtgttat tcaatgatac gtaaaatcac ggtatcataa agacttacgg
     2581 aggaatcaaa gcgtgctcaa gaaaccctgg gggggcaggt tcaaaaaaga tactcacaaa
     2641 atcgccgaga aattctccgc gtcgatcgac tacgacaaga gactctacaa agaggacata
     2701 gaggggagcg tcgcccacgt aaccatgctc gccgagaacg gcatcatact caagagcgaa
     2761 gccgaaaaga taataaaggg cctgaggcag atcgggaaag agatagattc ggggaagttc
     2821 cctttcaggg aggaatttga ggacatccac ctcaacatcg aaaagcggtt aatagaaaag
     2881 atcggggata ccggggggaa gatacatacg gcgaggagca ggaacgacca gatagctctc
     2941 gacatgaggc tctacctcag aaaagagata aacgagataa tacttctgat aaaagatatc
     3001 gccgtgtcgc tcatcggcct cgcggaaaag aacatagacg tcgtgctccc cctctacaca
     3061 cacctccaga ggggacagcc ggtcctcctg tcccaccacc ttatgggtct ctacgagatg
     3121 cttaagagag acagggaaag atacatcgac tgccgcgcga gagtaaacgt catgcctctc
     3181 ggcgcggggg cgggagcggg aaccacgttc ccgatagaca gggaactggt cgcgaggctc
     3241 ctcgatttcc ccaaagtcac gcagaacagc atagacacgg taagcgacag ggatttcatt
     3301 gtcgagttca tagccgtatc ggccaacctg atgatgcacc ttagcaggat gtccgaagag
     3361 ttcgtgctat ggtcgacgaa ggaattcgat ttcatcgatc taggggacga attcacgacc
     3421 ggttcgagca tcatgcccca gaagaggaac cccgacatgg ccgagctcat acgcggcaag
     3481 acagggagag tttacggcgg cctggtaacg atactgaccg tcatgaaagg cctccctttc
     3541 tcttacaaca gggacatgca ggaggacaaa gagcccatgt tcgatacagc cgacaccgtc
     3601 aagtcctcgc tcgaggtgct gagggcgatg ctgggcggca tcacatttaa gaacgaaaac
     3661 atgaaaaagg ctctcgctgc gggtttcatc acggcgaccg atatagcgga ctatctcacg
     3721 cgaaagggta tgcccttcag gaaatcgcac gaggtgaccg gaaagattgt gggatacgcg
     3781 gaggaaaaag ggagggagct catgaacctc cacatcaccg agtacaagat gttctcgagg
     3841 ctcttcgagg acgacctctt cgaccacatc acgctcgaag gctcagtcag cggcaggaaa
     3901 tccttcggcg ggacggctag gcagaacgtc gtcaggatga tagcgcaggg gaaaaaagag
     3961 acaggcaaat ggtgaatacg aattgaagga aatgaccgaa gccaaatata gagaattcct
     4021 cctccggggg accaagaccg gaaagctcgc gaccgtaagg aaggacggtc ggccgcacgt
     4081 cgtccccgta tggttcgaac tcgacggcga cgcggtcata tttccgacga gtgaccgctc
     4141 cgtaaaattt atgaacatga aacacgaccc cagggtatcc attacaatag acgaccagac
     4201 ccctccatat tcatacgtca tgatcgaagg cacggtttcg ttttcagaag accccggaga
     4261 gctcctctac tgggcgacgc gcatcggcgg gcgctacatg ggtgaagacc aggcggaagc
     4321 gtacggcaga aggaattcga ccccgggaga agtaatcgta agaataattc cgtcaaaggt
     4381 ttcggctttc gcggacgtcg ccggctggtg agtctatatc cctgaaccgt taccagccgc
     4441 tcttacgcta aacgctcgaa cttcttaaga atgggcaacc gcgaattccc cgacaattca
     4501 aatttcctat tgcgcatccg gccgatagga aagataatta aatgcggtcc cggagcgccg
     4561 gcattacgaa catttattca atcagagtag tcgctgtaag ccactgacaa cctggaggga
     4621 ctaatgaagc ttcactccga gaggaagcca gcaacacact ctaacatcac cagggtcatg
     4681 tcgaggtttc tctcggcgga gaaaaaatac agcccgattc tattctggtc cctcatagta
     4741 ggcgcactca ccggagctgc aggcgcggtc tttcagctct ctatagaaaa agtccaggac
     4801 tcgagagagc tcctgctgcg aatcatccct caaagtttcg tgttccagtt ggccgcttca
     4861 gtacttttct cggccgtttt agtttacatc gcctttcttc tcgtacggag attggcgcct
     4921 gaagcgagcg gaagcggagt ccacgagatc gagggggcgc tcgacgacgt aaggccgata
     4981 aggtggaaga acgtattgcc ggtgaaattc ctgggcggtc tctgctctct cggcggcggc
     5041 atggtcctcg gaagagaggg tccgacgata caaatgggcg ggaacatagg aaagatggtc
     5101 gcggatatct tcagggccgg caaggaagac acccacgcgc tgatcgcggc cggtgcaggt
     5161 ggcggattaa ccgcggcatt caacgcgcct ctcgccggca tacttttcgt attcgaggag
     5221 atgcgccccg aattcaaata caacttcctg tccgtacaat ctgtgat
//
LOCUS       DASSJH010000339         6016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1380416, whole genome shotgun sequence.
ACCESSION   DASSJH010000339 DASSJH010000000
VERSION     DASSJH010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380416"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VFJ67_07450"
     CDS             <1..905
                     /locus_tag="VFJ67_07450"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HET7290260.1"
                     /translation="RGARELIGIDVGRRRRESGGRLPVCVFRGREKGPSLLITAGLHG
                     DELNGIEAMRRMISDGSVIPDAGTVIALPMANVWGFLNSSRLTQDGRDLNRGFPGSRS
                     DAESQRIALAIMRHVLPMADFGIDLHSGGVSENFPHIRCTSSAPDDMELARAFAPPFI
                     LDSLPGEGTFRKAARLAGKCVLVYEGGRSGILSEFAVTECVNGLLRLMDNLGMIRTGA
                     PERNETRVISETQAVTSPCAGDYLPLVSCGAEVTKGKILASISGAENAGTITITSPAD
                     GFVLSVRPARRVNESDPLATIALH"
     gene            complement(1018..1461)
                     /locus_tag="VFJ67_07455"
     CDS             complement(1018..1461)
                     /locus_tag="VFJ67_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7290261.1"
                     /translation="MRVLLDTCVISELRKPDGNENVKRRILEIPDDNLYISVISIGEI
                     TEGVLCLADGKNKRALQSWLRSLERLYGDRILAIDLETVQIWGELTADAQKGGVTIPA
                     MDGLIAATARRHGLHVATRNTGDFDHTGAMLFNPWDDETRKRKSR"
     gene            complement(1458..1697)
                     /locus_tag="VFJ67_07460"
     CDS             complement(1458..1697)
                     /locus_tag="VFJ67_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008529504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET7290262.1"
                     /translation="MEWRLAEAKNKFSELVNRALEEGPQRVTRHKDAVIILSEEDYEK
                     LTGARPSFKKFLTEGSGLKGIDIKRDRSAMRDVDL"
     gene            complement(2107..2892)
                     /locus_tag="VFJ67_07465"
     CDS             complement(2107..2892)
                     /locus_tag="VFJ67_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290263.1"
                     /translation="MTEKNPESKYKAPAEGIAGGKSKKRSPNFPCIDLEKALELTDKI
                     YSVDKRAPIPVNLLIARWGFPKYNSYVRQLIAALTYYGLLESSGKGENRRVAVSDRGY
                     RILEKAPDRSKLLRTAALEPKLFNEVWQHYKQRGLPSNDVLERDLVWQNSFANTRFTK
                     NGAKMFISNLKSTVNFARILPDNVMYGEDNKSEVIDVMIHEQSGTASMGSFRRLDLTL
                     GSGEVATIQIPTSITLKEIRYLQDGINLIKGDLFPETKDDGSK"
     gene            3244..3504
                     /locus_tag="VFJ67_07470"
     CDS             3244..3504
                     /locus_tag="VFJ67_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290264.1"
                     /translation="MATKKPAKKAVKKAAKKPAKKAKSKMARHSVAFMATAKKAAPKK
                     KAAPKKKAAPKKTAKKAAPKKAAAKKKPAKKAAPKKKTAKKK"
     gene            complement(3600..4130)
                     /locus_tag="VFJ67_07475"
     CDS             complement(3600..4130)
                     /locus_tag="VFJ67_07475"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HET7290265.1"
                     /translation="MRRLYLHFKYQGARKGILAAYDKIARYFTGGPLTRFCRVTKDLW
                     IGGQPRGAGMRTLRARGITAVVNMRSEYDYPELAKLHELKYLRLPTEDNGAPALADLA
                     IGARFIADEIESGGRVYVHCWEGIGRSATMAAAYLVSKGSTPESAWKKIQGVRPFIRP
                     TAVQMKRLDEYARSRF"
     gene            complement(4159..5085)
                     /locus_tag="VFJ67_07480"
     CDS             complement(4159..5085)
                     /locus_tag="VFJ67_07480"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET7290266.1"
                     /translation="MRFRNVHVIINPASGNEPVDVEMIRSKLEASGQAFRISLTEPDT
                     GPGVLARRAVEEGADLVVAAGGDGTVLGTAEGLIWTGVPLGVIPEGTANVFAAEMGIP
                     SSSPDALGLLLGDGCEVRPIDVGAVGGRHFLLRVGIGIEAAMTVLTEPELKNRFGVLA
                     YMLTAYRHSREAGRTLYELDIDGRKMRMKGVTCVVCNSGNLGLPGVKLMPEIDPADGY
                     LNVVVVRQATLRALGSIVSNAMRGLISVKKEWGERRDINLYSVPAREVTVNPRPAQIA
                     ARDGEQIDSGFPLTITIEPKALLVAAPRKETI"
     gene            complement(5661..>6016)
                     /locus_tag="VFJ67_07485"
     CDS             complement(5661..>6016)
                     /locus_tag="VFJ67_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290267.1"
                     /translation="MCVPYHVDKEGRFYVHAGINWDYPPGEQPDPKIYYKDRKTYALL
                     AVLYEAGGYRFPYRDVYIGHTKTINERPDGTPVRRANLWNLDTGAGTYGKLTIMDADT
                     YEYWQSDWGEERARG"
BASE COUNT         1274 a         1810 c         1552 g         1380 t
ORIGIN      
        1 cgaggggcgc gagggaactg atcggcatcg acgtcggccg ccggagaaga gagagcggcg
       61 ggagactccc cgtctgcgtt ttcaggggca gggagaaggg cccgtctctc cttattacgg
      121 cagggctcca cggggacgag ctgaacggca tcgaggcgat gagacgcatg atctcggacg
      181 gctccgtgat ccccgacgcg ggcacggtca tagcgctccc catggcgaac gtctgggggt
      241 tcctaaattc ctcccggctc acgcaggacg gcagggacct caaccggggc ttccccggct
      301 cgcgctcgga cgcggaatcg cagaggatag cgctcgccat aatgcgtcac gtcctgccca
      361 tggcagactt cggcatcgac ctccattcgg gaggcgtgag cgaaaacttc cctcacatca
      421 ggtgcacgtc aagcgcgcct gacgacatgg agctcgcccg cgcattcgcc ccgcccttca
      481 tactcgactc ccttcccggt gaaggcacgt tcagaaaggc agcgcgcctc gcgggcaagt
      541 gcgtgctcgt ttatgaagga ggccgctcgg gaatcctttc ggaattcgcc gtcactgaat
      601 gcgtgaacgg attgctcagg ctgatggata atctcggcat gatacgcacg ggcgctcccg
      661 agaggaacga gacacgggtc atatcggaaa cacaggccgt aacgtcacca tgcgccgggg
      721 attatttgcc gctcgtctcg tgcggcgctg aagtaacgaa ggggaaaata cttgcgagca
      781 tatccggcgc ggagaacgcc ggcacgatca cgataacctc ccccgcggac ggcttcgtcc
      841 tctcggtcag acccgcccgc cgcgtgaacg aatccgaccc cctcgccaca atcgccctgc
      901 attgacggga catttactaa attgccggcg ggatttatcc aatacaacta aaacgataca
      961 aataaaatgc agatgacatg aagtgccggc acgatgagga aacttcacag cctctcttta
     1021 tcgtgatttt ctttttcgcg tttcatcatc ccagggattg aagagcattg cgcccgtatg
     1081 gtcaaaatcc ccggtattgc gggtcgcgac atgcagacca tggcggcgcg ccgtggcggc
     1141 gatcaaaccg tccatggccg ggatggtgac gccgcccttc tgagcgtcgg cagtcagctc
     1201 gccccatatc tggacggtct caaggtctat ggcaagtatc ctgtctccgt aaagccgctc
     1261 aagagaacgc agccagctct ggagcgctct tttgtttttc ccgtccgcaa gacacaacac
     1321 gccttctgtg atttctccga tggatatcac gctgatatac aaattgtcgt ccggaatctc
     1381 gaggatgcgc cgctttacgt tttcatttcc gtccggcttt cgaagctcgg aaatgacgca
     1441 tgtgtccagc agcactctca caggtccaca tcccgcatgg ccgaacggtc gcgcttgatg
     1501 tcgatgccct tcaggccgga cccttccgtc agaaattttt tgaaagaggg gcgcgcgccc
     1561 gtcagcttct cgtaatcctc ttcggaaaga atgatcacgg catccttgtg acgcgtaacg
     1621 cgctgcggcc cttcttcgag ggcccggttt accagctcac tgaatttgtt tttcgcttct
     1681 gccagccgcc attccatgag cacctccttg gctagattgt ctagctatat tatgacacgc
     1741 cccgtctaaa agggcaaggg aatttttata gaaaactctt tcagctttgc tgcgcgaaaa
     1801 ttcccgataa cagcaccgca cgtgcatacc agtgcagaaa ctaacccgcc aatccccctg
     1861 ccagaaccgg ctttccggcc tgtgctacat gcaccacctg tcagtttccc tgctaaaccg
     1921 gccgcccccg gaccgggtta ctttaacttt catccacaag cctcacatat ccttggacgg
     1981 acaattttca cccccgtcct gtccgtcaaa aggagggttt tctgtctgat tctgtgccgt
     2041 cgatttttac aggaaattta ccgggaggcc ctttttacgg gctttttggc gcggaaggac
     2101 cggagctcac ttgctcccgt cgtcctttgt ctcggggaaa aggtcccctt ttataagatt
     2161 tattccgtcc tgaaggtatc ttatctcctt caaggttatg gaggtgggta tttgtatggt
     2221 ggctacctcg ccgctcccca gggtgaggtc gagcctcctg aacgatccca tggaggcagt
     2281 cccgctctgc tcatggatca tcacgtctat cacttcggac ttgttatcct ctccgtacat
     2341 cacgttgtca ggtaaaatcc tggcaaaatt gacggtagat ttcagattgc tgatgaacat
     2401 cttcgccccg tttttggtga acctcgtgtt ggcgaagctg ttctgccaga cgaggtcccg
     2461 ctcgagcacg tcgttgctcg gaagacccct ctgcttgtag tgctgccaca cctcgttgaa
     2521 gagctttggc tcgagggccg ccgtcctgag cagcttcgac ctgtctgggg ccttctccag
     2581 tatcctgtac cccctgtccg acacggccac cctcctgttc tcccccttcc ccgaggactc
     2641 taacaaaccg tagtaggtaa gggccgcaat aagctgtctg acatagctat tatatttggg
     2701 gaatccccac ctggctatga gcaggttcac gggtataggg gcgcgcttgt ccacggagta
     2761 gatcttatcc gtcagctcga gcgctttttc caggtctatg cacgggaaat taggactgcg
     2821 cttctttgac ttcccgccgg ctattccttc agccggcgcc ttgtatttcg attcgggatt
     2881 tttctcggtc atgatgccgg ctctccggta taaaaggtgt aaagaaaatt gctccgataa
     2941 atataatccg tttgacgtag aaatcaagca aggctgacag ataaattaac aggtaaaaac
     3001 atttgacaaa aatctgtcag tgttgtatta tgtcagtaat ttcactgaaa ggaggattgc
     3061 atagatgaga aaatagaccc ccgtcataca agtgcaataa ggttgtcgtt gcatgacggg
     3121 tgttttaaag gagcagcggt ggttatagtc ggcccgatat aacgaatctg ctgcgagaca
     3181 accgggtcag taagtctatt atcttttatc tgtaaaaatc cactttaaac aggagattag
     3241 taaatggcta caaagaaacc agctaagaag gcagtaaaga aagcggcgaa gaagccggca
     3301 aagaaggcga agtcgaagat ggcgcgtcac tcagtagcgt tcatggcgac ggcgaagaag
     3361 gccgcaccga agaagaaggc tgcgccgaag aagaaagcgg ctccaaagaa gacagcaaag
     3421 aaagcggcac cgaagaaggc tgctgctaag aagaaaccag caaagaaagc ggcaccgaag
     3481 aagaagacag ctaaaaagaa gtaagcttta ccaaaggggc gtggtccctc acgccccttt
     3541 atttttccgg aggaaaaaaa gaatttaagt aatgtccccg acgggcccgg gtgacttcct
     3601 cagaaacgcg atctcgcgta ctcatccaat cgtttcatct gaacggcagt aggccttatg
     3661 aacgggcgca ctccttgtat ctttttccac gcgctctcgg gcgtgctgcc tttgctgact
     3721 aaatacgcgg cagccatcgt cgcgctcctc ccgatcccct cccagcagtg cacgtaaacc
     3781 ctgcccccgc tttcgatctc gtccgcgatg aatcgggcgc ctatggccag gtccgcaagc
     3841 gcgggcgcgc cgttgtcctc tgtcgggagc ctcaggtatt tgagctcgtg cagcttcgcg
     3901 agctcgggat agtcgtactc gctcctcatg ttgacgacgg cagtgatgcc ccgcgctctc
     3961 agcgtcctca tccccgctcc gcgcggctgc ccgcctatcc agaggtcctt cgtaacgcgg
     4021 cagaacctcg tgagcggccc tccggtgaag tacctcgcga tcttgtcgta agcggcaaga
     4081 atccccttcc tcgcgccctg atacttgaag tggagataga gtctccgcat ttcggtgaaa
     4141 aagctcatgt cttatttatt atatagtctc tttccgcggt gccgccacga ggagcgcctt
     4201 aggctcgatc gtaatcgtga gagggaaccc cgagtcgatt tgctcgccgt cgcgcgccgc
     4261 gatctgcgcg ggcctcggat tgaccgtgac ctcccgtgcc gggacggagt agagatttat
     4321 gtccctcctc tccccccact ctttcttcac ggaaatgagt cccctcatcg cgtttgaaac
     4381 gatagagccg agcgcgcgga gcgtcgcctg cctcacgacg acgacgttca ggtatccgtc
     4441 agccggatcg atctccggca tgagcttcac tcccgggagt ccgaggttcc ccgaattgca
     4501 cacgacgcac gtcacgccct tcatccgcat cttccgcccg tcgatgtcga gctcgtacag
     4561 cgtcctcccc gcttcccgcg agtgtctata cgctgtcaac atgtaggcga gcacgccgaa
     4621 cctgttcttg agctcgggct ccgtgagcac ggtcatcgcg gcttcgatcc ctatcccgac
     4681 ccgaagcagg aaatgcctgc cgccgacggc ccccacgtct atgggcctca cctcgcaccc
     4741 gtctccgagc aggaggccga gcgcgtccgg gctcgaggaa ggaatcccca tctccgcggc
     4801 gaacacgttc gccgtcccct cgggaatgac gccaagcgga acgcccgtcc atatgagccc
     4861 ttcggccgtc ccgagcacgg tcccgtcccc tcccgccgcc accacgaggt cggcaccctc
     4921 ttcaacggcc ctgcgcgcga ggactcccgg cccggtgtcc ggttccgtga gcgagatcct
     4981 gaaggcctgt ccggacgctt cgagcttcga ccttatcatc tctacgtcca cgggctcgtt
     5041 ccccgacgcc gggttaatta tcacgtgtac gttcctgaat ctcatctcgc acaattatac
     5101 cgattattat tcaacgatcc gcccaaatcc ccatccggtg agcattactc aaaattactt
     5161 ccctcccccc tagagggagg agggcctgcc ctgaactcgt ttcagggtca gatcgccggc
     5221 ccaacactga tattgcccgc cgtaaaacgg cgggcaacag ggacattacg ggatttgcgg
     5281 cgcagattct gcgctcatat ccgattctag tgaacctact ctgcctcccc actttgtcat
     5341 tgcggggagc gtagcgacgc ggcaatcccg ctttttttcc tttcccgaca ttacggaatt
     5401 tacgttaaga aaatcgattg tttacgcgtc aaaaacattt ttgggctgcg gtggaataaa
     5461 aatgttttag cgtaaatatg agatttttag taaattctgt gctgtcggat gatttttgct
     5521 tctattcatc aaggaaaaga aggatgaaaa caaatacttt tcactattct tcgtattttc
     5581 atcgaggaaa agaaggataa aaacaattgc ctttctcctt tccttgtctt ttcataagcc
     5641 gcacattgtg catccctacc ctatcccctc gccctctcct ccccccaatc gctctgccag
     5701 tactcgtacg tatccgcgtc cattatcgtg agcttcccgt acgtccccgc gcccgtatcg
     5761 aggttccaga ggtttgccct cctcacgggc gtcccgtccg gccgctcgtt tatcgtcttc
     5821 gtatgcccga tgtacacgtc cctgtaaggg aacctgtacc cgcccgcctc gtacagcacg
     5881 gcgagcagcg cgtacgtctt cctgtccttg taatatatct tggggtcggg ctgctccccc
     5941 ggcggatagt cccagtttat accggcgtgc acgtagaacc gtccctcttt gtcaacatga
     6001 tagggcacgc acatgc
//
LOCUS       DASSJH010000340         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1159257, whole genome shotgun sequence.
ACCESSION   DASSJH010000340 DASSJH010000000
VERSION     DASSJH010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159257"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..237
                     /locus_tag="VFJ67_07490"
     CDS             73..237
                     /locus_tag="VFJ67_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290268.1"
                     /translation="MSDKKFLDDVNPERREFLEKVTKGAFIIPTVISVMMLNQRLNLT
                     TANAQSNDAD"
     gene            424..1677
                     /locus_tag="VFJ67_07495"
     CDS             424..1677
                     /locus_tag="VFJ67_07495"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7290269.1"
                     /translation="MSARRSYGGCWPTPGQELLLRACILQGDEAAAAFQEWKASSDVN
                     TVDPGSYRLFPLLYKNLQTVGIDDPLLNIFRWVYERTLSNNRTLYGRLASFLRELNAR
                     GMPAILVKGSALALLYYPDPGLRPMMDADILVPTARAREAMEIITGLGWRSSVTPLKG
                     ISDARLLSRLGWTPGERGLGDYTDEYFSVRHGQDFTNPSQFTIDLHWHLLHGHNLPDA
                     DDEFWKGARSLSLGGVPALSLDPADQLLQVCSHGVAWSSIPPVRWVADAAAIIRKEGE
                     GLDWERLVRAAGSHGKVLPVRDALRYLERYLGTLVPERAILYLDSVPVPEAERFDYGV
                     RTRPPGVLAGLVELRFLWRSYSEENGDENIFAKISGFPKFLEHVFGMDSAWHLALYSG
                     YELVRRTGGILKSIKAKLLKTPAGD"
     gene            complement(1717..2121)
                     /locus_tag="VFJ67_07500"
     CDS             complement(1717..2121)
                     /locus_tag="VFJ67_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7290270.1"
                     /translation="MNSNRNIRSERYERGLVNLKKVDAESGEKVVESLGKISEDFATY
                     LIEYPFGDVYNRPGLDLRSREIATIAALTALGNARPQLLVHINAGLNVGLTREEVKEI
                     IIQMSVYSGFPSAINGLIAAAEVFDERDRQGK"
     gene            complement(2317..>2557)
                     /locus_tag="VFJ67_07505"
     CDS             complement(2317..>2557)
                     /locus_tag="VFJ67_07505"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET7290271.1"
                     /translation="IIARERYKPAGFKLPLGLKDMELALEAAEGTKTPMPALSMIHDH
                     LITAMARDYGDLDWSSIALLAAENAGLEVRLPESA"
BASE COUNT          522 a          734 c          722 g          579 t
ORIGIN      
        1 agcgaggggt atagttcgcg tagcttgagc ggggtatatt gattcattcc agcatgacgg
       61 gagattattc gtatgagtga caagaaattt ttggacgacg ttaacccgga aaggcgtgag
      121 ttcctggaga aggtcacgaa aggcgcgttc atcatcccga ccgtcatttc cgtgatgatg
      181 ctcaatcaga ggctcaacct cactacagcc aacgcccaga gcaacgacgc agattaatac
      241 ggaattccgg cccttacctc agcatccggt aaaataagac gcgccgtctg gttacgtcac
      301 gttatccgca ttatattatc cccacagggg gcaggttcgc cccttatacc gtttattatt
      361 gcaccagccg cttgtttcgt taccctcgat tagagataat agtcccgtcg tgggaaaaat
      421 atcatgagcg cgaggagaag ctacggaggc tgctggccta cgcccgggca ggagttattg
      481 ctcagggcat gcatcttgca gggagacgag gcggcggccg cgtttcagga atggaaggca
      541 tcgtccgacg tcaacacagt cgatcccggc tcgtacaggc tgtttcctct gctctacaag
      601 aacctgcaaa cggtcggtat tgacgacccg ctcctgaaca ttttcaggtg ggtctatgag
      661 aggacgctct ccaacaaccg gactctctac ggaaggctcg cctcgttcct ccgggagctt
      721 aacgcccgcg ggatgcccgc gatactagtc aagggctcgg cgctcgcgct cctctattac
      781 ccggacccgg gcctccgccc gatgatggac gctgacattc tcgtcccgac cgcgagggcg
      841 cgggaggcaa tggaaatcat cacgggtctc ggctggcgct cctcggtcac tccgctcaag
      901 ggcatatccg acgcgaggct tctgtcgagg ctcgggtgga ctcccgggga aaggggcctc
      961 ggggactata cggacgagta tttctccgtc aggcacgggc aggacttcac gaatccatcg
     1021 cagtttacga tcgacctcca ctggcacctc ctccacggcc ataacctccc tgacgccgac
     1081 gacgaattct ggaaaggcgc gcggagcctg agcttaggcg gcgtaccagc tctctcgctc
     1141 gaccccgccg accagctcct tcaggtttgc tcccacgggg tggcgtggag ctccataccg
     1201 cccgtgaggt gggtagccga cgccgctgcg atcatacgga aggaagggga ggggctcgac
     1261 tgggagaggc tcgtgcgcgc cgccgggagc cacggaaagg tgctgcctgt gcgggacgcg
     1321 ctcaggtatc ttgaacggta tctcgggact ctcgtgcccg aacgggccat cctgtacctt
     1381 gactccgttc ccgtcccgga agcggagaga ttcgattacg gagtgaggac gaggcctccg
     1441 ggcgtgctcg ccgggctcgt cgagttgagg ttcctctgga ggtcttactc ggaggagaac
     1501 ggcgacgaga atatattcgc gaaaatctct gggttcccca aattcctcga gcacgtattc
     1561 ggcatggaca gcgcctggca cctggctctc tattcggggt atgagctcgt gagacgcacg
     1621 ggcggaattc ttaagtcaat taaagccaaa ttgctaaaga ccccggccgg ggattaaaag
     1681 gatatctata tccgggtctt ccgacagtta taaggattac tttccctgcc ggtccctttc
     1741 gtcaaagacc tccgctgcgg cgataagccc gttaatggcc gaagggaatc ccgaatacac
     1801 ggacatctga attataattt ccttaacctc ctccctggtc agtcccacgt taaggccggc
     1861 attaatgtgc accaatagct gcggccgcgc gttcccgaga gccgtgagcg ccgcgattgt
     1921 cgcgatttct ctcgatctca ggtccagtcc cggacggtta taaacgtccc cgaacgggta
     1981 ttctatgaga taagtcgcaa agtcttctga tatcttgccg agactttcaa ccaccttttc
     2041 cccggactcg gcatccactt tcttcagatt caccagtcct ctctcatatc tttcgctgcg
     2101 aatatttcta ttcgaattca ttgcataacc tccccggcac gattgtagag tttaaacctg
     2161 taatcccgat tacgcccgtt cgtggcttat tcatttttgt atgtattctt tcgagttctt
     2221 tatagtgtcc gatcttctgc tctataagtc ttcagagcat ggcgggtagg agaagacggt
     2281 gggctcagtt aaggaagcgt gttcggtagg agcgcgtcac gctgattccg gaagcctcac
     2341 ctcgagcccg gcgttctcgg ccgcgagcag cgcgatagac gaccagtcga ggtccccgta
     2401 gtcccgtgcc atggcggtga tgagatggtc atggatcatg ctcagggcgg gcatgggagt
     2461 tttcgttccc tcggccgcct ctaacgcgag ctccatgtct tttaacccga gcggcagctt
     2521 gaagcccgcg ggcttgtacc tttcgcgggc gatgatc
//
LOCUS       DASSJH010000341         4805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1159557, whole genome shotgun sequence.
ACCESSION   DASSJH010000341 DASSJH010000000
VERSION     DASSJH010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159557"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..503)
                     /locus_tag="VFJ67_07510"
     CDS             complement(24..503)
                     /locus_tag="VFJ67_07510"
                     /inference="COORDINATES: protein motif:HMM:NF037752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290272.1"
                     /translation="MVPSFSDIALAQVPPCNVQICKVAPQLPPAFTGDDLVLFDFTEI
                     RGDSVTDFTLAANDNCTGGSYDLGESAVILEVPRDGWILADVDCTTTDGISTAILPDG
                     VQVHCLSEGFISCTFTNVQQISNIPALSEWGMIATAAGLALIGVFFAVRRKRLQDAL"
     gene            738..1250
                     /locus_tag="VFJ67_07515"
     CDS             738..1250
                     /locus_tag="VFJ67_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7290273.1"
                     /translation="MPKPKTLFLVRHAKSSWDEAGLDDRQRPLNERGKEEAPKMGKHL
                     AGYKVQPELITSSPAVRALKTAEKIAKELGFKKSDVVVEEGIYTFDGGSLMDVVKGFD
                     DRYMSVMMVGHNPAITSFANELSNAGIDNIPTCGVVLLEFDAGIWKDVGKGGGRVLEF
                     DYPKKLWGKA"
     gene            1300..1788
                     /gene="rraA"
                     /locus_tag="VFJ67_07520"
     CDS             1300..1788
                     /gene="rraA"
                     /locus_tag="VFJ67_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HET7290274.1"
                     /translation="MEHSTPDLCDLYPTLVRVAEPLFRNFGGRSSFGGEVVTIRCFED
                     NSFVKDKAGTPGRGKVMVVDGGGSVRKALLGDLIAETAMKNGWEGLIIFGAVRDVDSL
                     NGIGLGIKALASVPLKTERKGAGELNVPVTFAGVTFVPGEYVYADNTGIIVSAEPLGT
                     TA"
     gene            1936..3810
                     /locus_tag="VFJ67_07525"
     CDS             1936..3810
                     /locus_tag="VFJ67_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7290275.1"
                     /translation="MKLLLINPRFPESFWSFKWAVDNIMPGNIRTINPPLGLATLAAL
                     CPPDWEIEIIDENIETIPLNPKADIIGVCGMGVQFQRQKELLRFYRNRGFYVVAGGSY
                     ASLCPEYFETLADTVVAGEAEYIWKQFCSDFDIGSPQKLYKECSEVSLEDSPTPRFDL
                     LKLDKYQAVSLQFSRGCPYRCEFCDIIVMFGRKPRVKSFEQVGRELDELRKLGVTNVF
                     FVDDNLIGNKPVAKKLMAYLRDYQKEHNYKFLFGTEASLNIAQDDELLDLFTQANFGW
                     VFIGIESPDEESLKETLKFQNTRANILDSIRHVYSYGIEVLAGFIIGFDNDTLRTFDL
                     QYNFIQNSGIQAAMIGLLTAVPKTPLYERLEKDGRLIKDASGTDNTKLGTNLIPKGMT
                     YEEMVEGYRELYYRLLDDDTIADRINNKMKYMKNPIFRSTYGLTDQARSLTRFIRNGI
                     LPGGFSRVYHFLRTFPFTNPSMVPLVIQDWTIGISMRNYVDRHFIRVTEEEGSLARNH
                     FEALEKTFHRHLSKGHIELSLDRVRNTAHNLSISIKGMPGVEFFEHIAIHIENMLQNT
                     SSSVTLRIEELHEGEGHHLHRLIKRLSRYGDRTYIAVNEKLRDKVHIDSSVFNLVLEG
                     "
     gene            4038..>4805
                     /locus_tag="VFJ67_07530"
     CDS             4038..>4805
                     /locus_tag="VFJ67_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7290276.1"
                     /translation="MKDPFSIMKTAFGFWESKVLLTAVEMDLFTLLGPCSMTAEQIER
                     KLSLHPRGTYDFLDALVSMGFLDREGDGPGGLYKNTETTRYFLDKNGDSYVGGILEML
                     NARLFKYWDDLGDALKTGQPQNEIKHSQKPMFEELYADIPRLEQFMGAMTGLSRQNFV
                     ALAQKFDFSKYRTLCDVGGAAGVLSIAVAEKHRNLVCTSFDLPAVEPIAKRSIERTGM
                     SDRIKTASGDFFSDPLPKADVITMGMILHDWNLEKKKH"
BASE COUNT         1211 a         1350 c         1307 g          937 t
ORIGIN      
        1 tgaacggtac tattaataaa ttgtcataat gcatcctgta accgtttacg cctcaccgcg
       61 aaaaacaccc cgatcagagc caatcctgcg gccgtcgcga tcatccccca ttcggaaagg
      121 gcggggatgt tggaaatttg ctgcacgttg gtgaaggtgc atgaaatgaa accctcggac
      181 aggcagtgaa cttgcacgcc gtccgggagg atcgcggtgc ttattccgtc tgtagtggtg
      241 caatcgacat ccgcgagaat ccatccgtcc cggggaacct cgaggatcac cgcgctctca
      301 ccgagatcgt agctcccgcc cgtgcagtta tcgttcgctg ccagagtaaa atcggttacg
      361 gaatccccgc gtatttcagt gaaatcaaat aaaaccagat catccccggt gaaagcaggc
      421 ggcagctggg gggcgacttt acatatttgt acgttgcagg gcggcacttg cgcaagcgcg
      481 atatcggaga acgagggaac caataaaatt cctaacgcaa acagaaaaaa caggcaaacc
      541 ggcaattttt tattctgata cagtttcata tgaccatcct cctgcaatct acatcaaaaa
      601 cccatattac tacgatttac gtggatatgc aataatcttc atattttagc ttcatttccc
      661 atatttatcc cctccgctct attatattcc ttgccatccc ctttacggat gttagccttt
      721 aaaaagaggt aaatacaatg ccgaaaccta agacactctt cctcgtccgc cacgccaaat
      781 cgagctggga cgaagccggg ctcgacgaca ggcagagacc cctcaacgag aggggcaagg
      841 aagaggcccc caaaatggga aagcacctcg ccggatataa agtacaaccc gagctaataa
      901 cctcgagccc cgccgtgcga gccttgaaga cggccgagaa gatagcgaag gagctcgggt
      961 tcaagaaatc ggacgtggtg gtggaggagg ggatctatac cttcgacggg ggcagcctga
     1021 tggacgtggt gaaagggttc gacgacaggt acatgtccgt gatgatggtc ggccataacc
     1081 ctgcgatcac atccttcgcg aacgagctgt cgaacgccgg aatagacaac atccccactt
     1141 gcggcgtcgt gctcctcgaa ttcgacgccg gtatatggaa ggacgtaggg aagggtggcg
     1201 gcagggtcct cgaattcgac tatccgaaga agctctgggg aaaagcttga aatttccccg
     1261 cgcggctgta taaattcccc ttaatcagga taaaccggca tggaacactc tacccccgac
     1321 ctttgcgacc tataccccac tctcgtccgt gttgccgagc cccttttcag gaatttcgga
     1381 ggcaggagct ctttcggggg agaggtcgtg acgataaggt gcttcgagga caattcgttc
     1441 gtcaaggata aggcgggcac gcccggccgg ggcaaggtca tggtcgtcga cggcgggggc
     1501 tccgtgagga aagcgctgct cggggacctc atagcggaga cagccatgaa gaacgggtgg
     1561 gaggggctga taatattcgg agccgtgagg gacgtggact cgttaaacgg catcggcctc
     1621 gggatcaagg cgcttgcgtc cgtaccgctc aagacggagc ggaagggcgc aggcgagctt
     1681 aacgtgcctg tcacgttcgc gggcgtgaca ttcgtccccg gtgaatacgt ctacgccgac
     1741 aacacgggca taatcgtatc tgcggagcct ctcggaacga ccgcttgaga ctccgcccgg
     1801 acgcataaca tacgctttag cacccttccc gcagataacg cgcggctaaa acatattgcg
     1861 cacaaaggga aaatgaacta caatggaaac gggccttatt taattcaaac cggcccgggt
     1921 cagtgcggag gacaaatgaa acttttactc atcaacccga gattcccgga gagcttctgg
     1981 agcttcaaat gggccgtgga caacatcatg cccgggaata tcaggacgat aaaccctccg
     2041 ctcgggcttg ccacgctcgc cgccctctgt cctccggact gggagatcga gataatcgac
     2101 gaaaatatag agacgatacc gctcaacccg aaggcagaca taataggcgt ctgcggcatg
     2161 ggcgtccagt tccagaggca aaaagagctg ctgaggttct acaggaacag ggggttttac
     2221 gtcgtggccg gagggagcta cgcctccctt tgcccggagt atttcgagac gcttgccgac
     2281 acagtcgtcg ccggagaagc cgaatatata tggaaacagt tttgctccga tttcgatatc
     2341 ggctccccgc agaagctata taaggaatgc agcgaggtct cgctcgaaga ttcccccacg
     2401 ccgcgcttcg acctcctcaa gctcgataag tatcaggccg tgagcctcca gttctcgagg
     2461 ggatgcccct accgctgcga gttctgcgac ataattgtca tgttcgggag aaagccccgc
     2521 gtaaaatcgt tcgagcaggt ggggagggag ctcgatgagc ttcggaagct cggcgtcacg
     2581 aacgtattct tcgtcgacga caacctcatc gggaataagc ccgtcgcgaa gaagctcatg
     2641 gcgtatctga gggattacca gaaggagcac aactacaaat tcctcttcgg caccgaagcg
     2701 tctctcaata tcgctcagga cgacgagctg ctcgacctct tcacgcaggc gaatttcgga
     2761 tgggtgttca ttgggataga gtcgcccgac gaggagagcc tcaaagagac gctcaagttc
     2821 cagaatacga gagccaatat actcgactcc atcaggcacg tgtactccta cggcatagag
     2881 gtgctggccg ggttcatcat cgggttcgac aacgacacgc tccgcacttt cgacctccag
     2941 tacaacttca tacagaactc gggcattcag gccgctatga tagggctcct cactgccgtg
     3001 ccgaagaccc cgctatacga gaggctcgag aaggacggac gtctgatcaa ggatgcgagc
     3061 gggacagaca acaccaagct cggcacgaac ctcatcccca aggggatgac ctacgaggag
     3121 atggtcgagg ggtacaggga gctttattac aggcttctcg acgacgacac catagccgac
     3181 aggatcaaca ataaaatgaa atacatgaag aacccgatct tcaggagcac ttacggctta
     3241 accgaccagg cgcgctcgct cacgaggttc atccggaacg gcattctgcc gggcggcttc
     3301 tccagggtgt atcacttcct gaggacgttt cccttcacaa acccctccat ggtcccgctc
     3361 gtcatacagg actggactat aggcatctcc atgaggaact acgtcgaccg tcatttcata
     3421 agggtcacgg aagaagaggg gagcctcgcc aggaaccact tcgaggcgct cgagaagacg
     3481 ttccaccgtc atctgagcaa agggcacatc gagctctcgc tcgacagggt ccgcaacacc
     3541 gcgcacaacc tgtccatatc cataaagggc atgccgggcg tggaattttt cgagcacatt
     3601 gccatccaca tcgagaacat gctccagaac acgtcctcat cggtgactct caggatcgag
     3661 gagctgcacg aaggcgaggg ccatcacctc cacaggctca tcaagcgctt atcgcgctac
     3721 ggcgacagga catacatcgc cgtgaacgaa aagctccgcg acaaggtgca catcgactca
     3781 tcggtattca acctcgtgct cgaggggtaa cgcagaatct gtggcacaca atgtgtgctc
     3841 atatacgatt ctattgaatc ttctcttctt tgaccagcga acgaagccca acccgttcga
     3901 cccgtttgtc cggcatagcc ttggcgaagc cggaagctcg tcgaagggtt gaactggtat
     3961 cgctcttctc aagcaaccaa ccctaccttt tccgacatct acaaaaaacc gtcaaatttg
     4021 caggtaatat attgcccatg aaagacccct tttcgataat gaaaacggcc ttcgggttct
     4081 gggaatcgaa ggtgctactc acggccgtgg aaatggacct atttacgctg ctcggcccgt
     4141 gctcgatgac cgcggaacaa atcgagcgaa aactctcact ccacccgagg ggcacgtacg
     4201 atttcctcga cgcgctcgta tccatggggt tcctcgacag ggaaggggac ggcccgggcg
     4261 gactttacaa aaacacagag accacgcggt actttctgga caagaacggc gattcttacg
     4321 tgggcggcat actcgagatg ctgaacgcgc gcctgttcaa gtactgggac gacctcggag
     4381 acgcgcttaa aaccgggcag ccccagaacg agataaagca cagccagaag cccatgttcg
     4441 aggagcttta cgcggacata ccgcgcctcg agcagttcat gggggcgatg accgggctct
     4501 cgcgccagaa cttcgtagcg ctcgcccaga agttcgactt ctcgaaatac cgaacactat
     4561 gcgacgtggg cggcgcggcc ggggtcttat cgatcgccgt cgccgaaaag cacaggaacc
     4621 tcgtatgcac gtccttcgac ctcccggcag tcgagcccat cgcgaagagg tcgatagaga
     4681 ggacgggcat gtccgaccgc ataaagaccg cgtcgggcga cttcttcagc gacccactgc
     4741 caaaggcgga cgtgataacg atgggcatga tactccacga ctggaacctc gagaagaaga
     4801 aacac
//
LOCUS       DASSJH010000342         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1051427, whole genome shotgun sequence.
ACCESSION   DASSJH010000342 DASSJH010000000
VERSION     DASSJH010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051427"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFJ67_07535"
     CDS             complement(<1..376)
                     /locus_tag="VFJ67_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7290277.1"
                     /translation="MKRNEVRGRSSKKRLDTVLVDRGLAETRARARAVIMAGSVYVDG
                     VRTDKAGALVGEGSGVEVRDSSLKYVSRGGLKLEAALREFGIDPSGQVAVDIGASTGG
                     FTDCLLKSGASRVYAIDVGYGHL"
     gene            complement(376..1272)
                     /locus_tag="VFJ67_07540"
     CDS             complement(376..1272)
                     /locus_tag="VFJ67_07540"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET7290278.1"
                     /translation="MKFVIKSYLAEKRELVDGELDRLLTDGSVNRGRLLESMRYSALA
                     GGKRLRPILAIAAGEAVGAEPYRVLPAACAIEMIHTYSLIHDDLPSMDDDALRRGVPT
                     NHRVFGESTAILAGDALLTDAFGILVGEGLRRGVDARVVCEVVRDLAQAAGSRGMIEG
                     QVIDLALAGNKGVTLGEVERMHALKTGAMIRASVTAGARIGGADESQLGKLAAYADGV
                     GLAFQIIDDILDIEGEGDLGKERGNDERHGKSTYPGVAGIDESRKKASELTRQAVRAL
                     EGFDHRADALRQIALWLGCRNN"
     gene            complement(1279..1506)
                     /gene="xseB"
                     /locus_tag="VFJ67_07545"
     CDS             complement(1279..1506)
                     /gene="xseB"
                     /locus_tag="VFJ67_07545"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7290279.1"
                     /translation="MKSEKTFEDALRELKEIIKKLESGNLPLEDSIKLFQEGTELISF
                     SHKKLNEVRKKVEILAEKNGEITFEDFEPES"
     gene            complement(1615..>2013)
                     /locus_tag="VFJ67_07550"
     CDS             complement(1615..>2013)
                     /locus_tag="VFJ67_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290280.1"
                     /translation="FPDMPEGVTRKSCVLLSKQLEREGITIWINTAAQVSHPAPNGIH
                     HFFIRALAEGRDAVLYDDLNPPAALNKSYFKKFISHFSKIVINRKKVGLPVWQTPIAM
                     GIMLTYYSLALAGAVITSVMPGYAKSSWRI"
BASE COUNT          365 a          640 c          516 g          492 t
ORIGIN      
        1 cgagatgtcc gtacccgacg tctatcgcgt acactctcga agcccctgat tttaagagac
       61 agtccgtgaa cccgcccgtg gacgcgccta tatctacggc gacctgtccc gaggggtcta
      121 ttccgaattc cctcaacgcc gcttccagct tgagcccgcc gcggctcacg tacttgagcg
      181 agctgtccct gacctctacc ccggagcctt ccccgacgag agcgcccgcc ttgtccgtcc
      241 tcacgccgtc cacgtaaacg gagcccgcca ttattacggc cctcgccctg gctctcgttt
      301 ccgcgaggcc cctatcgacg agcaccgtat cgagcctctt cttcgaagaa cggcctcgga
      361 cttcattcct tttcatcagt tgtttctgca gccgagccag agcgctattt gtctcagcgc
      421 atcggccctg tggtcgaacc cctcgagcgc gcggactgcc tgacgcgtga gctcggaggc
      481 cttttttctg gattcatcga tccccgcgac gccggggtaa gtggacttgc cgtgcctctc
      541 gtcgttcccc ctctccttcc cgaggtcccc ctcgccttct atgtcgagta tgtcgtctat
      601 tatctggaaa gcgagcccta cgccgtcggc gtatgcggcg agcttcccga gctgactttc
      661 gtcagcgcct ccgatgcgcg cccccgccgt gacggacgcc cttatcatcg cgcctgtttt
      721 gagcgcgtgc atgcgctcca cctcgcctag cgtgactccc ttattccccg cgagcgccag
      781 atcgatgacc tgtccctcta tcatcccgcg cgagcccgcg gcttgcgcga ggtctctcac
      841 cacttcgcac acgacccgcg catcgacccc tctcctcagc ccctccccga cgagtatccc
      901 gaaggcgtcc gtgaggagag cgtccccggc gagtatagca gtcgattccc cgaataccct
      961 gtggttagtc gggactcctc ttcttaacgc gtcgtcgtcc atggaaggga ggtcgtcgtg
     1021 tatgagggaa tacgtgtgta tcatctctat agcgcaggca gccggaagga cccgatacgg
     1081 ctcggccccc actgcctctc ccgccgcaat cgcgagtata gggcggagcc tctttccccc
     1141 ggcgagcgcg ctgtacctca tcgattcgag gagcctcccc cggttcacgc tgccgtccgt
     1201 cagcagcctg tcgagctctc cgtccacgag ctcccgcttt tcggcaagat acgatttaat
     1261 gacgaatttc atgagtcctc agctttcggg ctcgaaatcc tcgaaggtta tctcgccgtt
     1321 cttctcggcg agtatctcca ccttcttcct gacttcattg agttttttat gcgagaacga
     1381 gatgagctcc gtcccttcct gaaagagctt tatggagtct tcgagaggca ggttgccgct
     1441 ctcgagcttc tttattattt cctttagctc cctgagcgcg tcctcgaaag tcttttcgga
     1501 tttcatagag aggtagttta tctctgaagc ggactccgca caaggagtgt agggttattg
     1561 gccccgttca gcggcgtcga gaacgccgtg agccgcgtaa tttcaatatc cctgctagat
     1621 tctccatgaa gatttggcat acccgggcat caccgaagta ataacggctc cggccagtgc
     1681 caacgaataa tatgtgagca taatgcccat ggcgattgga gtctgccata cgggtagacc
     1741 gacttttttt ctgttaatga cgattttgga gaagtgggaa ataaattttt tgaaatagga
     1801 tttatttaat gctgcgggcg ggttgagatc atcgtacaat acagcgtcac ggccctcggc
     1861 gagtgccctt ataaaaaagt gatgaatccc gttaggcgcg gggtgggaga cctgggccgc
     1921 cgtatttatc caaatcgtta ttccttcccg ctccagttgt ttcgagagca acacacatga
     1981 tttacgtgtt acaccttccg gcatgtcggg aaa
//
LOCUS       DASSJH010000343         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_408604, whole genome shotgun sequence.
ACCESSION   DASSJH010000343 DASSJH010000000
VERSION     DASSJH010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408604"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFJ67_07555"
     CDS             <1..518
                     /locus_tag="VFJ67_07555"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET7290281.1"
                     /translation="PDLRAGAYDDNPIPVGMGQTISQPYIVALMTELLNPVSGMKVLE
                     VGTGSGYQSAVLAETGCELYTIEIVESLAMKARTILERIGYSNIAYRIGDGYQGWEEH
                     APFDGIIVTAAPGHVPPRLIEQLKVGGRMVIPVGDESQELLLIEKTETGITKKRITAV
                     RFVPMTGESES"
     gene            complement(558..1559)
                     /locus_tag="VFJ67_07560"
     CDS             complement(558..1559)
                     /locus_tag="VFJ67_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7290282.1"
                     /translation="MYSRTILAVFLLTGIALAQGSLPPPKGGTTIYVVEPGDSLWKIS
                     GRFFGNPLFWPRLWEINPYIDNPNRIYPGDVIALKAQQPDIPVVKVEPKAHKVSFEDI
                     EPPPPVYYYSQGGSEGFITPGEWEHMGTILTSEPPKILLGTGDIVFTNVGSKNGVDVG
                     DMFTIFRTSKPVFHPITGQKIGYKVQIEGELELLEILGKRKSTAVITSSYLEITRGAR
                     VRPREPFVKEVIMRKGTQRVDGFIVDNKNNTELSGKGDIVYIDAGEVNNVVPGNVFSI
                     YTQPRKAHDPDAHKDVIIPGTLIGKIVVLEVKEKSATGIITESSRQIEIGDVVSLDI"
     gene            1753..>2289
                     /locus_tag="VFJ67_07565"
     CDS             1753..>2289
                     /locus_tag="VFJ67_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HET7290283.1"
                     /translation="MDNRILINITFNETRVAVMENGAVAELYIERKSTPRIVGNIYKG
                     VVGKVVPGMQAAFIDIGLGKSGFISVEDVHEDSLYEYFLDGGSESQQSRKHDRGLIQD
                     ILREGQHVLVQVLKESVGGKGAKLSSNIGIPGKYSVLLGTTDIVGISRKIEDPEERTR
                     LGEIIREIKPEGKGIIART"
BASE COUNT          522 a          604 c          636 g          527 t
ORIGIN      
        1 cccctgattt acgggcgggc gcgtacgacg ataaccctat ccccgtcggc atggggcaga
       61 ctatctctca gccctacata gtcgcgctga tgacggagct cctcaatcct gtaagcggca
      121 tgaaggtgct cgaggtgggg acgggctcgg gttatcagtc ggctgtgctg gctgagaccg
      181 ggtgcgagct atacacgata gagatcgtcg agagtcttgc catgaaggcg cgcacaattc
      241 ttgaaaggat aggctattcc aacatcgctt acaggatcgg cgacggatac cagggctggg
      301 aggagcacgc tcctttcgac ggcataatag tcacggctgc cccggggcac gtgccgccga
      361 ggctcattga acagctcaag gtaggcggga ggatggtcat tcccgtaggc gacgagagcc
      421 aggagcttct gctaatagaa aagaccgaaa ccgggattac aaagaagaga ataaccgctg
      481 tccgcttcgt acccatgacg ggtgagtccg aatcctgagc acggggttgg ggcgcccggt
      541 cgaagcggat cgattgctta tatatccagg ctcaccacgt cacctatctc gatctgtctc
      601 gagctctcgg tgattatgcc tgtcgcgctt ttctccttga cctcgagcac gacgatcttt
      661 cctataagag tgcccgggat gatgacgtct ttatgcgcgt ccgggtcgtg ggccttccgc
      721 ggctgggtgt agatagagaa tacgttaccg ggtactacgt tattgacttc acccgcgtct
      781 atataaacga tatcgccctt tccgcttaac tcggtgttgt tcttattatc gactatgaac
      841 ccgtccaccc tttgcgtacc cttcctcatg ataacctctt ttacgaaggg ctcccggggc
      901 cttaccctcg ccccgcgcgt tatttcgagg tatgaagaag tgatgacggc ggtcgatttc
      961 ctcttgccga gtatttccag gagctcgagc tctccttcga tctgcacctt atacccgatc
     1021 ttttggcccg tgatcggatg gaatacgggt ttcgaggtgc ggaatatggt gaacatgtcg
     1081 cccacgtcca ctccgttctt agaccccacg ttcgtaaata cgatatcgcc cgtgccgagg
     1141 agtatcttcg gcggctccga tgtgagaatg gtccccatgt gctcccactc gcctggggtt
     1201 ataaatcctt ccgatccgcc ctgcgaataa taatagacgg gcggaggcgg ctctatgtcc
     1261 tcgaaagaca ccttgtgcgc cttgggctct accttgacta cggggatgtc gggctgctgg
     1321 gccttgagcg ctataacgtc cccggggtat atgcggttcg ggttgtctat gtacggattg
     1381 atttcccaga gcctcggcca gaagagcggg ttgccgaaga acctgccgga gatcttccag
     1441 agcgaatccc ccggctccac tacataaatc gtcgtcccgc cctttggggg cggcagactg
     1501 ccctgggcga gcgcaatacc ggtcaaaagg aatacggcta agattgttct tgaatacatc
     1561 ccgtctatcc tccgttaaga cccgtaatcc ggcagggccg gccgggttct taatgcgtta
     1621 tccaaatttt acaccctttt caaacgaaat gaaagtatta ttatgttctt ttaggctttc
     1681 atataaagaa cttatagact atacttaaga acgcttgtaa ggtttggacg ctttcggaca
     1741 gaatttcgtc atatggacaa caggatactt ataaatatca cattcaacga aacgagggta
     1801 gccgtcatgg aaaacggcgc cgtcgccgag ctttatatcg agcgcaagtc cacgccgcgc
     1861 atagtgggga atatatacaa gggcgtcgtc ggaaaggtcg taccgggaat gcaggcggcg
     1921 tttatcgata taggcctcgg aaagtcgggt ttcatatccg tcgaggatgt gcacgaggac
     1981 tctctttacg aatacttcct cgacggcgga agcgaatccc agcagtcccg gaagcacgac
     2041 aggggactca tacaggacat actccgggag ggacagcacg tcctcgtcca ggtgctcaag
     2101 gaatctgtcg gagggaaggg tgcgaagctc tcctccaaca tcggcattcc cggcaaatac
     2161 tccgtacttc tgggtacgac cgatatagtc gggatctcga ggaagataga agacccagag
     2221 gaaaggacca ggctcggaga gatcataagg gaaataaaac ccgaggggaa aggtattata
     2281 gcgagaacc
//
LOCUS       DASSJH010000344         5850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1490403, whole genome shotgun sequence.
ACCESSION   DASSJH010000344 DASSJH010000000
VERSION     DASSJH010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490403"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFJ67_07570"
     CDS             complement(<1..1082)
                     /locus_tag="VFJ67_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290284.1"
                     /translation="MDGTNSPPIGNARITASRKLGIIGMFFAAYLLLLPVMSAASQQH
                     KEYNTPPDKDRPTTVYIGLYLLDMITLDQPKQMLRARYALSLHWKDPRAAFESPDNKP
                     DIVIGDEVDERIKYMWWPDPQIVNQLSGITEINRRLLVHPDGNIYYSSVFVVDTATTL
                     EFRKFPFDSQTIRIEFESFSFPKDELDYKVWGERTGVNVNIQNDEWILHDISSTAGIR
                     RLWESEDSDWSYVSFRLPIHRRTAYYVWRIIFPFCFFILISKCVYWMKRESLDRRVSI
                     VVSFALTAVAFNFVVSGFFPNVSYLTILDAIILSGYLMCGITIISVISFELFKHHGKP
                     EVWYYGNTAAKFMFPLLYILTWYLLY"
     gene            complement(1246..1323)
                     /locus_tag="VFJ67_07575"
     tRNA            complement(1246..1323)
                     /locus_tag="VFJ67_07575"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1286..1288),aa:Asp,seq:gtc)
     gene            complement(1340..1416)
                     /locus_tag="VFJ67_07580"
     tRNA            complement(1340..1416)
                     /locus_tag="VFJ67_07580"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1380..1382),aa:Val,seq:tac)
     gene            complement(1735..4158)
                     /gene="lon"
                     /locus_tag="VFJ67_07585"
     CDS             complement(1735..4158)
                     /gene="lon"
                     /locus_tag="VFJ67_07585"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7290285.1"
                     /translation="MFFKNEEGKGQMETIPLLPLRDVVIFPYMVAPLFVGREKSIRAL
                     EEAMKKSKEIFLVAQRDAKTNDPQETDIYDIGTIGTIVQMLRLPDGTVKVLVEGKKRA
                     KVLNYLPNKGYFLVEVEVIPEPQEVGVETEALIRTLVTAFENYVKLNKKIPSEVLVTV
                     SSIEDPGRLSDTISSHLGFKLEDKQEILETLDPAERLEKLYEKIQAEVEILQIEKKIR
                     SRVKKQMEKAQKEYYLTEQMRAIQKELGEKDDLKSEIQEFEERLKKKTMPEEAEQKVK
                     KEIKKLKQMSPMSAEATVVRNYIDWLLSIPWGEEKTEDRIDIDEAQKILDEDHFGLEK
                     PKERILEYLAVQALTSKIKGPILCFVGPPGVGKTSLAKSIARSMGRKFVRVSLGGVRD
                     EAEIRGHRRTYIGALPGKIIQGVRKAGTTNPVFLLDEIDKLGMDFRGDPASALLEALD
                     PEQNVAFNDHYIEVDYDLSNVMFITTANVLHTIPWALQDRMEIIRISGYTEDEKLEIA
                     KKFLVPKQLEAHGLKDEGLKISDAAILTVIRRFTREAGVRTLERELASLCRKAARDIV
                     KKGPDHVVKITPNMITSQKYLGIPKYKYGEIEEKPQVGISTGLAWTEVGGELLTIEVS
                     VVPGKGNFTVTGKLGEVMQESTQAAMSYVRSRAERLGLERNFHQKVDIHIHVPEGATP
                     KDGPSAGIAIGTAIVSALIKKPVKHDVAMTGEITLRGRVLPIGGLKEKILAAHRGKVK
                     TVIIPQENEKDLKDIRENILKDVKIKLVDHMDEVLEAAIESDGPVIQSVVIPPAALPM
                     GDELGTNVN"
     gene            complement(4197..5474)
                     /gene="clpX"
                     /locus_tag="VFJ67_07590"
     CDS             complement(4197..5474)
                     /gene="clpX"
                     /locus_tag="VFJ67_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET7290286.1"
                     /translation="MTSRRDAKEGKLYCSFCGKSQKEVRKLIAGPTVYICNECIELCN
                     EIIADGDAKEGVLKKRYSALPTPSQIKKFLDEYVIGQVRAKKILSVAVYNHYKRVELN
                     AYNGGKRNSDVEVQKSNILMIGPTGSGKTLLAQTLARLLDVPFTIVDATCYTEAGYVG
                     EDVENMIVSLLQAADYDLERASKGIIYIDEIDKLARKTGDSPSITRDVSGEGVQQALL
                     KIMEGTKANVPPKGGRKHPQQEFLQVDTTNILFICGGAFCGLEDIVKQRIGEKSIGFE
                     ANLGRGKEKDLGALLREVQPEDQIQFGLIPEFVGRIPVVATLEELSEESFVEILTKPK
                     NAIIKQFQKLMELEGVELEVTDGALAAIAREAISRKTGARGLRAIIETVMLDISYEVP
                     SLKGLRRCVITEDVINGKGDPLFIYEEEARLGT"
     gene            complement(5471..>5850)
                     /locus_tag="VFJ67_07595"
     CDS             complement(5471..>5850)
                     /locus_tag="VFJ67_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET7290287.1"
                     /translation="GLAIYDTMQYIKHDVATMCLGQAASMAEVLLAAGTKGKRYALPH
                     ARVMLHQVLGGVEGQATDIEIHAKEILRMREELNRILAKHTNQPIDRISKDTERDYFL
                     RPNDALEYGIVDAIISKREEAAK"
BASE COUNT         1151 a         1730 c         1401 g         1568 t
ORIGIN      
        1 tgatataaca ggtaccacgt taatatatac agcagcggga acatgaattt cgccgccgtg
       61 ttgccgtagt accatacttc cggtttcccg tgatgtttaa aaagctcgaa acttataacc
      121 gaaattatcg tgattccgca catcaagtat ccggatagta ttatggcgtc cagaatcgtc
      181 aggtaagaca cgttggggaa aaaccccgag accacgaaat taaaagctac cgcagtcaag
      241 gcaaaagaga ctactatgct gacgcgcctg tcgagcgatt ctcttttcat ccagtacacg
      301 catttcgaaa tcagtatgaa aaagcagaac gggaaaatta ttctccagac gtaatacgcg
      361 gttctcctat gtatcgggag gcggaaactg acgtatgacc aatcactgtc ttccgattcc
      421 cagagcctcc tgatacccgc ggtagacgag atatcatgga ggatccattc gtcgttctga
      481 atattgacat tcacgcccgt cctctcgccc cagactttat agtcgagctc gtctttggga
      541 aatgaaaacg actcgaactc gattctgata gtctggctat cgaaaggaaa tttcctgaac
      601 tcgagcgtcg tagcagtgtc gacgacaaac accgacgagt agtatatatt tccgtccgga
      661 tgaacgagca gcctcctgtt tatctccgtt attccgctca gctgattaac gatctgcggg
      721 tcgggccacc acatgtattt aatgcgttca tcgacctcgt ctcctataac tatgtccggc
      781 ttattatccg ggctctcgaa cgcggccctc gggtctttcc agtggaggga cagggcgtac
      841 cgggcgcgga gcatctgctt cggctggtca agcgtgatca tatcgagcag gtagagcccg
      901 atgtaaacgg tcgtcggcct gtccttatcg ggaggggtat tgtactcttt atgctgctgt
      961 gaagcggcgc tcataaccgg gagaagtaag aggtatgcgg cgaagaacat acctataatg
     1021 ccgagcttcc tcgaagcagt tatacgcgcg tttccaatcg ggggtgagtt cgtaccgtcc
     1081 atcgcagaaa gctctctttt ctccgggtca gcaggatcag gcagattgcg cccacatacc
     1141 gctatcgaaa aatatattaa atattacatc aaaatagaat ggaaataccc atggcggatt
     1201 aaagattttc ggtatttccg acagttgagg aatctatgga aaaactggcg gggacgacgg
     1261 gacttgaacc cgcgacctct ggcttgacag gccagcgttc taaccggact gaactacgtc
     1321 cccgcaagtt agtttggagt ggtgggcggt aaaggatttg aacctatgac atcctgcttg
     1381 taaggcaggc gctctcccat gctgagctaa ccgcccactc cgaatttgag tatttcagtc
     1441 aatcacaccc ggcgcccgta agggagaaat atataaccga attttacccg tttgtccaca
     1501 cacacgtagt gtgtttctat gatcctaatg gaactcccta gtgctgccgg catcctgtat
     1561 atctgcggtg acccgtccgg ccttagtccc tacggtgcag aacctgcgct ggtatacgat
     1621 tcttccatac cttctccaca caacccggcg acgaagccca acccgttcgg gccaaaaaaa
     1681 agggcggtta tttctaaccg cccctttaac ttaatcttct ttaatttcta tctcttaatt
     1741 tacgttcgta ccgagctcat cgcccatggg caatgccgcc ggaggaataa cgacgctctg
     1801 gattacgggc ccgtccgact ctatcgccgc ttcgagcacc tcgtccatgt ggtctacgag
     1861 cttgatcttg acgtccttga gtatgttctc gcggatgtct ttcaggtcct tctcgttctc
     1921 ctgcgggatg atgactgtct tcaccttgcc cctgtgagcg gcgagtatct tctctttaag
     1981 cccgcctatc gggagcactc tccctctgag cgttatctcg cctgtcatgg ccacgtcgtg
     2041 cttgacgggt ttctttatga gcgccgatac tattgcggtc ccaatggcta tgcccgccga
     2101 cggcccgtct ttgggtgtcg cgccttccgg aacgtggatg tgtatgtcca ccttctgatg
     2161 gaagttacgc tcgagcccca gcctctcggc cctcgacctc acgtagctca tcgccgcctg
     2221 agtggactcc tgcatgactt ctcccagctt tccggtaacg gtgaagttac ccttgccggg
     2281 cacgacggat acttctatgg tgagcagctc tccgccgact tccgtccacg cgagccccgt
     2341 ggatatgccg acctgcggct tctcctctat ctcgccgtac ttgtacttgg gtatgcccag
     2401 gtacttctga ctcgtgatca tgttaggtgt tatcttcact acgtgatccg gccctttctt
     2461 cacgatgtcc ctagcggcct tcctgcaaag cgaggcgagc tctctctcga gagtcctcac
     2521 tccggcttcc ctcgtaaagc gtctgataac cgtgagtatg gcagcgtcgg aaatcttcaa
     2581 gccctcgtcc ttgaggccgt gcgcctctag ctgcttcggg actaagaatt tcttcgctat
     2641 ctcgagcttc tcgtcttccg tgtaccccga gattcggatg atctccatcc tgtcctggag
     2701 cgcccacggt atcgtgtgaa gcacgttcgc ggtcgtgatg aacattacgt tagaaaggtc
     2761 gtaatcgacc tctatgtaat ggtcgttgaa ggcgacgttc tgctccgggt cgagcgcttc
     2821 gagaagcgcg gacgccgggt cgcccctgaa gtccattccg agcttgtcta tttcatcgag
     2881 caggaacacc gggttggtag tccccgcctt tcttactccc tgtattatct ttccgggcag
     2941 agcgccgatg tatgtacgcc tgtggcccct gatctcggct tcgtccctta cgccgcctaa
     3001 cgacaccctc acgaacttcc ttcccatcga gcgggcgata gacttggcta aagacgtctt
     3061 gccaactccc ggaggcccga cgaagcagag tatcggcccc tttatcttgc tcgtaagggc
     3121 ctgtaccgcc aggtattcga gaatcctttc cttgggcttc tctaacccga agtggtcctc
     3181 gtcgagtatc ttctgagcct cgtcgatgtc gatcctgtct tcggtcttct cttctcccca
     3241 cggtatcgag agcagccagt cgatgtagtt ccttacgact gtcgcctcgg ccgacatcgg
     3301 ggacatctgt ttcagcttct taatctcttt cttgaccttc tgctcggcct cttcgggcat
     3361 ggtcttcttc ttgagcctct cctcgaattc ctgtatctcc gatttgaggt cgtccttttc
     3421 gccgagctcc ttctggatgg ccctcatctg ctcagtgagg tagtactcct tctgggcctt
     3481 ctccatctgc ttctttactc tcgaccgtat cttcttctcg atctggagta tctccacctc
     3541 ggcctgaatc ttctcgtaga gtttctcgag cctctcggcc gggtcgagcg tttcaagtat
     3601 ctcctgcttg tcctcgagct tgaacccgag atgagaggag atagtgtccg aaagcctgcc
     3661 cgggtcctct atcgaggaaa ccgtgacgag cacctcggac ggtattttct tgttcagttt
     3721 tacgtagttt tcaaatgcgg tgacgagcgt ccttatgagc gcttcggtct cgacgcctac
     3781 ttcctgaggc tcgggtatga cctcgacttc gacgaggaag taccccttgt tcgggaggta
     3841 gttgagtacc ttcgccctct tcttgccctc gaccagcacc ttgacggtgc cgtccgggag
     3901 cctcagcatc tggactatcg tgccgatcgt ccctatgtcg tatatgtcgg tttcctgagg
     3961 atcgttcgtc ttcgcgtccc tctgagcgac gaggaagatc tctttcgatt tcttcatcgc
     4021 ctcttcgagc gcgcgtatcg atttctcgcg ccccacgaac agcggcgcga ccatgtacgg
     4081 aaaaataact acatctctta gcggtaataa cggtattgtt tccatttgcc cctttccctc
     4141 ttcattttta aaaaacatcg gcatggcatg tctcctttac tatccggatt cacatcctac
     4201 gttcccaggc gcgcctcctc ctcgtatatg aagagagggt cgcccttccc gttaattacg
     4261 tcctccgtga tgacacaacg cctaagccct ttaagcgacg gaacctcgta cgatatgtcg
     4321 agcattacgg tctcgatgat cgccctcagc cctctcgcgc ccgttttcct cgaaatagcc
     4381 tcgcgggcga ttgcggcgag tgcgccgtcc gttacctcga gctcgacacc ttcgagctcc
     4441 atcagcttct gaaactgttt tataatcgca tttttcggtt tggtgaggat ctccacgaac
     4501 gactcttcgc tgagctcctc taaagtcgcc acgaccggta tcctgcccac gaactccgga
     4561 atgagcccga actggatctg gtcctcgggc tgcacttccc ttagcagcgc ccccaggtcc
     4621 ttctccttcc cccttccgag gttggcttcg aacccgatcg acttctcacc tattctctgt
     4681 ttcactatgt cttccagccc gcaaaaagcc ccgccgcaga taaacaatat gttcgtcgta
     4741 tccacctgca ggaactcctg ctgggggtgt ttcctcccgc ccttgggcgg aacgttcgct
     4801 ttagtcccct ccatgatctt gaggagggcc tgctgaacac cctcgccgga aacgtctctc
     4861 gttatagacg ggctgtcccc ggtctttctc gcgagcttgt ctatctcatc tatatatata
     4921 atacccttgg aagccctttc aaggtcataa tctgcagcct ggagcagact gacgatcatg
     4981 ttctcgacgt cctcccctac gtatcccgcc tccgtgtagc aggtggcgtc aacgatggtg
     5041 aacggcacgt ccaggagcct ggccagcgtc tgcgcaagca gcgtcttccc gctccccgtg
     5101 ggtcctatca tcagaatatt gcttttctga acctcaacgt cggaattcct tttgccgccg
     5161 ttgtaagcgt tcagctcgac tcttttataa tggttataga ccgcgacgga gagtatcttc
     5221 ttggccctca cctgcccgat cacgtactcg tcgaggaact tctttatctg cgaaggcgtt
     5281 ggtaaagcgg agtaacgttt cttgagtacg ccctctttcg cgtctccgtc ggcaataatc
     5341 tcgttacaga gctctataca ctcattgcat atatatacgg taggtcccgc aatgagtttc
     5401 cgcacttctt tctggctctt cccgcagaaa gagcagtaca gttttccttc tttggcatcg
     5461 cgtctcgaag tcacttggcc gcctcctctc tcttagaaat tatagcatca acgataccgt
     5521 attctaaggc gtcatttgga cggaggaaat aatccctctc cgtgtccttc gatatcctgt
     5581 cgataggctg gttcgtatgc ttcgccagaa tacggttcag ctcctcgcgc atcctcaata
     5641 tttccttcgc gtgtatctct atatcggtcg cctgcccctc gacgcccccc aatacctgat
     5701 ggagcatcac gcgcgcgtga gggagagcgt acctcttgcc cttcgtcccc gccgccagga
     5761 gcacctccgc catgctggcg gcctgcccga ggcacatggt cgcgacgtcg tgctttatgt
     5821 actgcatagt gtcgtagata gcgaggcccg
//
LOCUS       DASSJH010000345         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_300300, whole genome shotgun sequence.
ACCESSION   DASSJH010000345 DASSJH010000000
VERSION     DASSJH010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300300"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFJ67_07600"
     CDS             complement(<1..383)
                     /locus_tag="VFJ67_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET7290288.1"
                     /translation="MRVEIERLAFGGSGVARKDGKVYFVKGGLPGDSVYIRVINDRGR
                     FAEAVIESIAEPSPERVEPPCDVFYSCGGCQLQNLGYAAQLREKENILGETVRRIGGL
                     KDVCPEPIFSSPSEYGYRRRVLLGA"
     gene            complement(400..1407)
                     /gene="queA"
                     /locus_tag="VFJ67_07605"
     CDS             complement(400..1407)
                     /gene="queA"
                     /locus_tag="VFJ67_07605"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET7290289.1"
                     /translation="MKTSDFDYHLPEERIAYRPAAERQNSKLMVLGRGTGEITHRKFF
                     ELPGLLRPGDLLVLNDTKVIPARLFGRKESGEKAEILLAGKRDCRTWTCLVRNPKEGL
                     VIEFEGGPSGRLFRGKRGEWLIEFVEEPDAYIDASGRMPLPPYIRREPDAGDRVTYQT
                     VYARKPGAIAAPTAGLHFTPGLLEEIGLRGVRTAYVTLHVGIGTFLPVKAESVEEHVM
                     HAEWREIPAETADAVNKAKSGGGRVIAVGTTVLRTLESSVNEAGEVAPCNGETDLFIL
                     PGHRFKAADALITNFHLPRSTLLMLVTAFAGRDPIFRAYEEALGLDYRFLSYGDAMLI
                     E"
     gene            complement(1419..>2245)
                     /locus_tag="VFJ67_07610"
     CDS             complement(1419..>2245)
                     /locus_tag="VFJ67_07610"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7290290.1"
                     /translation="FELTFYALDPSIKVIAPWREWDLNSRSALIEYAEKHGIPVPVTQ
                     QKPYSCDRNLFHTSYEGGILEDPWSEPPENMFEMTVSPERAPDDPVYLDIDFEKGVPV
                     KVDGKALGPVELLTVLNKIGGANGVGRVDLVENRFVGMKSRGVYETPGGTILHTAHRA
                     LESLTMDREVMHMRDALIPRIAELIYYGFWYSPEMDMLMASVEESQKNVTGTARLKIY
                     KGNIILAGRRSPYSLYKEDLATFEKDSIYNQADATGFIKLNALRLSVRKLLEKKGK"
BASE COUNT          431 a          713 c          568 g          533 t
ORIGIN      
        1 cacgcaccga gcagtacgcg cctcctgtat ccatactcgg acggggagga gaatatgggt
       61 tcggggcaca cgtccttgag cccgcctatc ctcctcaccg tctctccgag gatattttcc
      121 ttctccctga gctgagccgc atagccaagg ttctgaagct gacaccctcc gcatgagtag
      181 aaaacgtcgc acggcggctc gaccctttcg ggagagggct ctgctatact ctctatcacg
      241 gcttcagcga atctgcccct atcgttaatc acgcgtatat agacgctatc accgggcagt
      301 ccccccttca cgaaatagac cttcccgtcc ttcctggcga cgccgctccc gccgaatgca
      361 agcctctcta tttctacacg caacctaatc tcccctgtac tattctatga gcatggcgtc
      421 cccgtaactg aggaacctgt aatccagtcc gagagcttcc tcataagccc tgaagatagg
      481 atccctgccg gcaaacgcgg taacgagcat gaggagcgtg gacctgggca gatgaaagtt
      541 cgtgataagg gcgtcagccg ctttgaatct gtgacctggc agtatgaaga ggtccgtttc
      601 cccgttgcac ggtgctactt cgcccgcctc attcaccgac gactcgagtg tcctcagcac
      661 ggttgtcccg acagctatca cccttccgcc cccggacttc gccttgttga ccgcatcggc
      721 agtctcagcc ggtatctccc tccactcggc gtgcattacg tgttcttcca cggactccgc
      781 cttcacaggc aggaacgtac ctatcccgac gtggagagtt acgtacgcgg tccgcacacc
      841 tctgagcccg atctcttcaa gaagccccgg tgtgaagtgg agtcctgcgg taggggctgc
      901 gattgctcca ggcttcctcg catatacggt ctggtaagta accctgtccc cggcgtcggg
      961 ctcacgtctt atgtatgggg gaaggggcat cctgcccgag gcgtctatat acgcgtcagg
     1021 ttcttcgacg aattctatca accattcgcc ccgcttacct ctgaagagcc ttcccgacgg
     1081 gccgccctcg aactcgatca cgagcccttc tttcggattc cttacgaggc atgtccacgt
     1141 acggcagtcc cgttttcccg ccagtagtat ttcggccttc tctcccgatt ctttcctccc
     1201 gaagagcctt gccggtatca ccttcgtatc attgaggacg agcaggtcac cgggcctaag
     1261 caagcccggc agctcgaaga acttcctgtg agtaatctcg cccgtccccc tgccgaggac
     1321 catcagcttc gaattttgcc tttccgcagc gggacggtat gcgatccgct cctccggaag
     1381 atgataatcg aagtccgagg tcttcatcgg gcgagtgatt acttcccctt cttttcgagg
     1441 agtttccgaa cggagagcct gagtgcattg agctttataa agccggtcgc gtcagcctgg
     1501 ttatatatgc tgtccttctc gaaagtagcc aggtcttcct tataaagcga atacggtgag
     1561 cgtctgccgg ccagtatgat attacccttg taaatcttca gtcttgccgt accggtcacg
     1621 ttcttctgag attcctcgac cgaggccatc agcatgtcca tttccggcga ataccagaat
     1681 ccgtagtaaa tgagctccgc gatcctcggg atgagcgcgt ccctcatgtg catgacttcc
     1741 ctgtccatgg tcagcgattc gagcgccctg tgggcggtgt ggagaatagt accgcccgga
     1801 gtctcataaa ccccgcgtga tttcatgccg acgaacctgt tctcgacgag atcgaccctc
     1861 cccacgccgt tcgcgccgcc tatcttattg agcacggtga gaagctcgac agggccgagc
     1921 gcctttccgt cgacctttac cggcactccc ttctcgaaat ctatgtcgag atacacagga
     1981 tcgtccgggg ccctctccgg cgagaccgtc atctcgaaca tattttcggg cggctccgac
     2041 cacgggtctt cgagtatccc accctcgtag ctcgtatgga aaaggttcct gtcgcagctg
     2101 tagggtttct gctgagtcac agggacgggg attccgtgtt tctccgcgta ttctatgagc
     2161 gcgctcctcg agttcagatc ccactccctc cacggcgcta tgacctttat cgaagggtcc
     2221 agggcgtaaa acgtgagctc gaacc
//
LOCUS       DASSJH010000346         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1490445, whole genome shotgun sequence.
ACCESSION   DASSJH010000346 DASSJH010000000
VERSION     DASSJH010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490445"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFJ67_07615"
     CDS             <1..561
                     /locus_tag="VFJ67_07615"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET7290291.1"
                     /translation="VLASSSPRRTELLRATGLAFEVIHPFSDETVSGGEAPEEFALRV
                     SLEKACSVSSSLGDGAVVIGADTIVVVDGEILGKPGDPEEASSMLRKLSGREHKVYTA
                     FSIVRAKNELLHSEAVSTAVRVIPLAASEIEGYIKTGEPMDKAGAYAIQGAGSFIVSG
                     IEGSYSNVVGLPVEELLEALKKLEII"
     gene            575..1264
                     /locus_tag="VFJ67_07620"
     CDS             575..1264
                     /locus_tag="VFJ67_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7290292.1"
                     /translation="MDVAGSLRSVKERIGEAAARAGRPAGGIKLVAVTKGVEPARIVE
                     AIKAGHLTFGENYAQEFRLKKEIIDRTVDERVEWHFIGRLQRNKVKYLVGEVGLIHSL
                     DSLSVAHEIEKKAAGMGIRVPVLIEVDTGGEETKGGVKSEEVEGFIGSLVELGSIEVR
                     GLMTMPPYFDDPVKARPYFTALRELRDRLRAGHPGLTELSMGMSGDFEAAVEEGATIV
                     RIGTTIFGPRQ"
     gene            1284..2099
                     /gene="proC"
                     /locus_tag="VFJ67_07625"
     CDS             1284..2099
                     /gene="proC"
                     /locus_tag="VFJ67_07625"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET7290293.1"
                     /translation="MAKIGFIGGGNMAEALIKGLISSGVYKKGDIIVSDNVKKRLDHL
                     KAAYGVAVTQDNKVVASSSDIIVLSVKPNTVRKVVGEIKSRITAKKILISIAAGIPVS
                     LISGVLKKKAKIVRVMPNTPALVLAGASALYCSPNLGEDEKERVKRIFEAVGIAYIIE
                     DEKLLDTVTGLSGSGPAFVSMFIESLSDGGVKMGLPRDAALRLAAQTVYGTAKMIIEE
                     MSHPAVLKDKVSSPGGTTIEGIHKLEAGGFRANVISAVEAATKRSRQLSKEDK"
BASE COUNT          531 a          542 c          663 g          432 t
ORIGIN      
        1 gtcctcgcat catcgtcccc gaggcggacc gagctcttgc gtgcgaccgg tctcgcgttc
       61 gaggtcatac accctttctc ggacgagacg gtctcaggcg gcgaagctcc cgaggaattc
      121 gctctcaggg tctcactcga aaaagcttgc tccgtgtcat cgtctctcgg cgacggcgct
      181 gtcgtcatag gagccgatac gattgtcgtc gtcgacggcg agatactcgg caagcccggc
      241 gaccccgagg aggcttcctc gatgctgcgg aagctctcgg gaagagagca caaggtatat
      301 accgcttttt ctatcgtgag ggctaaaaac gagctcctcc acagcgaggc tgtctctacg
      361 gctgtcaggg tgataccgct tgcggcttcc gaaatagaag gttacattaa gaccggagag
      421 cccatggata aagccggggc gtatgcgata cagggcgcgg gctctttcat tgtaagcggc
      481 atagaaggct cgtactctaa cgtcgtgggt ctccccgttg aggagctcct cgaagcatta
      541 aaaaaacttg aaataattta gatattctga aaggatggat gtagcaggca gtctcaggtc
      601 cgttaaggaa aggattgggg aagcggcggc cagggcagga aggcccgcag gcggcataaa
      661 gctcgttgcc gttaccaaag gggtcgagcc cgcgcgtatt gtcgaggcaa taaaagcggg
      721 ccacctgaca ttcggcgaga attacgcgca ggagttcagg ctcaagaagg aaataatcga
      781 ccggacggtg gatgagagag tcgaatggca tttcatcggg cggcttcagc ggaacaaggt
      841 taaatatctg gtgggggagg tcggattgat acactcgctc gattcactct ccgtcgcgca
      901 cgagatagag aagaaggccg cgggcatggg tatccgcgtc ccggtgctaa tagaggtcga
      961 tacgggcggc gaggaaacga aaggcggagt gaagagtgag gaagtggaag gcttcatcgg
     1021 tagtctcgta gaattaggct cgatagaggt ccgcgggctc atgaccatgc ctccttattt
     1081 cgacgatccc gtgaaagcgc gtccttactt tacggctctc agggagttac gcgacaggct
     1141 ccgggctgga catccgggat tgaccgagct ctcgatgggc atgagcggcg attttgaagc
     1201 tgctgtcgag gaaggtgcta cgatagtcag gataggcacc acgattttcg ggccgaggca
     1261 atgacactca ggcggataaa gaaatggcga aaataggctt cataggcggc gggaacatgg
     1321 cggaggcgct gatcaagggc cttatatcgt caggggtgta taaaaaaggc gacataatcg
     1381 taagcgataa cgttaaaaaa agattagacc acctgaaggc agcttacggc gtcgccgtca
     1441 cacaagataa caaggtcgtc gcatcctctt ccgacatcat agtcctttcc gttaagccga
     1501 acacggtcag gaaggtggtc ggcgagataa agtcgagaat tacggcgaag aagatactga
     1561 tctcgattgc ggccgggata cctgtctccc ttatatcagg cgtactgaag aagaaagcga
     1621 agatagtccg cgtcatgccc aacacgcccg cgctcgtgct ggccggggcc tccgcccttt
     1681 actgcagtcc caatcttggt gaggatgaga aggagcgggt aaaaaggata ttcgaggccg
     1741 taggcatcgc ctacatcata gaggacgaga agctcctcga cacggttacc gggctcagcg
     1801 gcagcggccc cgctttcgtg tccatgttca tcgagtcgct ctctgacgga ggcgtaaaga
     1861 tgggccttcc ccgggacgcg gcgctcaggc tcgcggccca gactgtttac ggcactgcga
     1921 aaatgattat cgaggaaatg tcccacccgg ctgtgctcaa ggacaaggtc tcctctccag
     1981 ggggcacgac gatagagggc atacacaagc tcgaggcggg agggttcagg gctaacgtga
     2041 tttccgccgt cgaagcggcc acaaaacgct cgaggcagct ctcgaaggag gataaatagt
     2101 gttcatactt gcaaacctcg taataggcgt aggacagacc atccattaca tcataaatat
     2161 ttatatct
//
LOCUS       DASSJH010000347         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_408888, whole genome shotgun sequence.
ACCESSION   DASSJH010000347 DASSJH010000000
VERSION     DASSJH010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408888"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFJ67_07630"
     CDS             complement(<1..435)
                     /locus_tag="VFJ67_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290294.1"
                     /translation="MSPRIILLSLAAVFMVFMPLTADANKLFIANIKVDDDVVFEGTD
                     SLFKVVDFFDNKSLNKFFPDYTSNSAVLARVDLRGIGLEIEYRQNSSELIVRIPAKHF
                     EVRFNGGNRNESQEQFEDWLKGDFESLEAPGRKLTTLLHIVVA"
     gene            complement(648..1319)
                     /locus_tag="VFJ67_07635"
     CDS             complement(648..1319)
                     /locus_tag="VFJ67_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290295.1"
                     /translation="MSKNIYGVLHSFLFLIILYSVSCATITVNVYFPAEEVREAYSNL
                     EEEFLEQGSKGDSGAEKKQPDAIKPAPTPAPQSMRKYQEEPTLSSEKKYLVIKREISL
                     DFSNYAWAQGNVAQQITQKIKSMPDVIQAYKRRSQRLNEINQMLSSGLVTEGGQGLLV
                     QKGILTPSQTQAFNAENSDRQVIIRGMAKAIVEINNMDPTPENIEKVLPEAARQFASV
                     RQSER"
     gene            complement(1333..>2081)
                     /locus_tag="VFJ67_07640"
     CDS             complement(1333..>2081)
                     /locus_tag="VFJ67_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290296.1"
                     /translation="EGGNALTSLVNDKLTLDRDLYNEYFRTFKEPAPDGGVDHIKIDE
                     IEYGILKFDNVDCALMADTEKLAIKKLSADIFGGRIYSSGDLRFGERGGLYNFSFLFN
                     DISLDAISKRLSPTEEYITGRLNGLVWLSGEGAELGTVDGPFEFWSVSSQKEPRSIGK
                     ALLDKLGAKERFILGSSRSYDNGEISGYINDGVITFKKFNISNSVLGIKNLSIQADPV
                     KNSISISHLVSVIREIARRSQSGGPTIETQ"
BASE COUNT          463 a          567 c          505 g          546 t
ORIGIN      
        1 ggccacaact atgtgcagga gcgtagtaag cttccggcca ggagcctcaa gggattcgaa
       61 atctccttta agccagtcct cgaattgctc ctggctctca ttgcggttac cgccgttgaa
      121 cctgacctcg aaatgtttgg caggtatcct gacgattagc tccgatgaat tctggcgata
      181 ctctatttcc aggccgatgc ctctcagatc gacccgagcc agtaccgcgg aattagacgt
      241 gtaatcgggg aagaatttgt taagggactt attgtcaaaa aaatcgacta ccttgaacaa
      301 actgtcggta ccttcgaata cgacatcgtc gtcaaccttg atattggcga taaacaattt
      361 attggcatcg gccgtgagcg gcataaaaac cataaacacc gccgctaaag acagcaggat
      421 aattctcggg ctcatgtctc atcctccgga cggcgcattt tgggaagagg gccgtcttgg
      481 caccaataat tagacaacaa ttgaggagga acttcaatat gtgtgaattt taatttgcgg
      541 attgcagcgg gagagggccg ttaccccctc tcccggaatt ttttcgcgaa ctgtattaca
      601 accgttggtg aacggttgga cttaacattc gaaggtcaac tatgccctca cctctcgctc
      661 tgtctcacgc tcgcgaactg ccttgcagcc tcgggcagaa ccttttcaat gttctcgggc
      721 gtcgggtcca tattgttgat ctcgactatc gccttggcca tgcctcttat aatcacctgt
      781 cggtcagagt tctcggcgtt aaaggcctgg gtctgcgagg gagtgaggat gcctttctgg
      841 acgagaagcc cctggccgcc ttccgtaaca aggcccgatg aaagcatctg gtttatctcg
      901 ttcaaacgct gcgaccttct tttataagcc tgaatgacgt cgggcatgct ctttattttc
      961 tgcgtgatct gctgcgctac gtttccctga gcccacgcgt aattgctgaa atcgagggaa
     1021 atctcccttt ttataaccag atatttcttc tcggacgaaa gagtcggctc ctcctggtac
     1081 tttctcatgc tctgcggggc cggcgtaggc gcgggcttta tcgcatcagg ctgttttttc
     1141 tcagcacccg aatcgccctt ggagccctgc tccaggaact cttcctccag gttcgaatac
     1201 gcctctctca cctcttctgc cgggaagtag acgttcaccg ttatagtcgc gcatgaaacg
     1261 gaatagagta ttatcagaaa aagaaatgag tgcagaacgc cgtatatatt tttactcata
     1321 aatttcacct cgtcattgag tttctatagt cgggccgccc gactgcgacc gcctggctat
     1381 ttcccttatt acggacacca gatgcgagat cgatatcgaa ttcttcaccg ggtccgcctg
     1441 tatcgacagg ttctttatac ccagtacgga gttggatata ttgaattttt tgaacgttat
     1501 cacgccgtca ttaatataac ctgaaatctc cccgttatca tagctgcggg aagagccgag
     1561 gatgaacctc tccttcgccc cgagcttgtc gaggagcgcc ttaccgatag acctgggctc
     1621 cttttgcgag ctcacggacc agaactcgaa gggcccgtct accgtgccga gctcggcgcc
     1681 ctcccccgag agccatacga gaccgttcag cctccccgtt atatattcct cggtcggcga
     1741 cagccgtttg gaaatagcgt cgaggctgat atcgttaaag aggaacgaga aattatacaa
     1801 gccgcccctt tcgccgaagc ggagatcccc gctcgaatat atgcgccctc cgaatatatc
     1861 cgccgagagc ttcttgatag cgagcttttc cgtgtcggcc attagcgcgc agtcgacgtt
     1921 gtcgaatttc agtattccgt attctatctc gtcgatcttg atatgatcga ccccgccgtc
     1981 gggagccggt tccttgaagg tgcggaaata ctcgttgtac aggtccctat cgagcgtcag
     2041 cttatcattg acgagtgacg taagggcgtt tccgccctcc c
//
LOCUS       DASSJH010000348         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_618623, whole genome shotgun sequence.
ACCESSION   DASSJH010000348 DASSJH010000000
VERSION     DASSJH010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618623"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFJ67_07645"
     CDS             complement(<1..433)
                     /locus_tag="VFJ67_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7290297.1"
                     /translation="MRFEPDFVHIYVPPSDPGSPSTLLLLHGTGGDEESLVPIAERIL
                     PGAGILSPRGKVLENGMPRFFRRFSEGVFDLEDLRVRTRELAEFIAKASEIYSFDRNK
                     LIAAGYSNGANIAASVLLSFSRVIPFAVLFHPMVPFVPDVLP"
     gene            complement(442..1374)
                     /locus_tag="VFJ67_07650"
     CDS             complement(442..1374)
                     /locus_tag="VFJ67_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HET7290298.1"
                     /translation="MPENILGIHHITAIATSAKKNHAFYTEVLGLRLVKKTVNFDDPT
                     TYHFYYGNERGEPGTILTFFPWEGIRRGREGTGQVTAASFSVPGDSKSFWLNRFRANN
                     VIYNNPSSRFGEEVLVFLDPDGLKLELVFDNGDGRAAWDADEVPVEFGIRGFRGAALT
                     LDGYEATANLLTGVLGYEKKEEHVNRFRFVNGNSKNANIIDLICLPSGQRGSVAGGTV
                     HHIAFRAANEADQLALRDKLISLGYNVTPQLDRNYFKSVYFREPGGVLFEIATDPPGF
                     TIDEELKDLGHSLKLPGWLEPRRDEIESVLPGVG"
     gene            complement(1401..1751)
                     /locus_tag="VFJ67_07655"
     CDS             complement(1401..1751)
                     /locus_tag="VFJ67_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290299.1"
                     /translation="MKITRIYSDDEGESHFGELDIALADSGPIGRLSARLQAGSIIFR
                     ENDAGYDYDWHHAPERQYILLLDGEIEIEVSDGEKRIFRGGDVIFVEDTEGRGHRTRV
                     TNNRPRRSVFVTLL"
     gene            complement(1778..2149)
                     /locus_tag="VFJ67_07660"
     CDS             complement(1778..2149)
                     /locus_tag="VFJ67_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004595075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7290300.1"
                     /translation="MTDEERKSIAAEYLKSLDYGRDFFNLFAEDAEVFFPNWGVARGA
                     VEYKRLFSDLGARVAEFIHHHAYINWIIQGDMVVAEGSSEGKLKTGESWTDSKWCDVF
                     EIRDGKIRRLYIYLDPEYSRY"
     gene            complement(2185..>2683)
                     /locus_tag="VFJ67_07665"
     CDS             complement(2185..>2683)
                     /locus_tag="VFJ67_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HET7290301.1"
                     /translation="LIDKSIFRIYRDTRFSSDKTPYKTHLGILFWDGPRKKLENPNYY
                     VQINKSSIFVGAGEYIFPPDLLRSYRDSVVHPGRGSELKKILDGIVANPSYKIGGRHY
                     KRVPRGYDPEHPNAELLHHNGLYAYSESALPEDIYSRRFIDYCFDIFRDMYPLQRWLA
                     RANHL"
BASE COUNT          562 a          753 c          712 g          656 t
ORIGIN      
        1 cgggcagaac gtccggcacg aaggggacca taggatggaa gagcacggca aagggaatca
       61 cccttgaaaa cgagaggaga acgctcgccg ctatgttcgc accgttcgaa tagccggctg
      121 ctatcaattt attcctgtcg aatgaatata tctccgaggc cttcgctatg aattcggcga
      181 gctcccgagt cctcacgcgc aggtcctcga ggtcgaagac cccttccgag aagcgcctga
      241 agaacctcgg catgccgttt tcaaggactt tgccgcgcgg actcagtatg cccgcgccag
      301 gcagaatcct ctccgcaatc gggacgaggc tctcctcgtc gcccccggtc ccgtgaagga
      361 ggaggagcgt gctcggtgac ccgggatctg acggcgggac gtatatgtga acgaaatcgg
      421 gctcgaatct catgtattcc tctaaccgac ccccgggagg acgctttcta tttcatccct
      481 cctgggctcg agccatcccg ggagcttaag cgaatgcccg aggtccttga gctcctcatc
      541 gatggtaaag ccgggcgggt cggtcgctat ctcgaaaagg acgccgccgg gctcgcggaa
      601 gtagaccgat ttgaagtagt tcctgtcgag ctgcggggtg acgttgtatc cgagagatat
      661 gagcttgtct ctcaaggcga gctggtcggc ttcgttagct gccctgaacg ctatgtggtg
      721 aaccgtgcct cccgcgacac tcccgcgctg tccggagggg aggcatataa gatcgataat
      781 attcgcgttc ttactgtttc cgtttacgaa cctgaagcgg ttgacgtgct cttctttctt
      841 ttcatatccg agtacgcctg tcagtaaatt cgcggtcgcc tcgtaaccat cgagagtgag
      901 ggccgcgccg cggaagcctc ttatgccgaa ttcgacaggg acctcgtccg cgtcccatgc
      961 cgctctcccg tctccgttat caaatacgag ctcgagcttc agaccgtcgg gatcgaggaa
     1021 gaccagaact tcctctccaa acctcgaaga gggattgtta taaataacgt tgttggctct
     1081 gaaacgattg agccagaagc tcttcgaatc cccggggact gaaaaagagg cggcagtcac
     1141 ctggcccgtg ccctcccttc ctctccttat gccctcccag ggaaaaaacg tgaggatagt
     1201 tcccggctcg cctctctcgt tcccgtaata aaaatgatac gtcgtcgggt catcgaaatt
     1261 gacggtcttt ttaacgagcc tgagaccgag tacctcggtg tagaatgcgt ggttcttttt
     1321 agcgctcgtt gcaatcgcgg taatatgatg gattccaagt atattttcag gcatgtatcc
     1381 ctcgcagatg aatcaatcgg tcagaggagc gtgacgaaca cggaccgcct cggacggtta
     1441 ttcgtgaccc tcgtcctgtg ccccctccct tccgtgtcct cgacgaatat cacgtccccg
     1501 ccgcggaata tccttttctc cccgtcgctg acttcgatct ctatctcgcc gtcgagcagc
     1561 aggatgtact gcctctcggg ggcgtgatgc cagtcgtaat cgtaaccggc gtcgttttcc
     1621 ctgaagatga tggagcccgc ctggagcctg gctgatagcc tgcctatggg gccggaatcg
     1681 gcgagggcta tatccagctc gccgaagtga gattctccct catcgtccga gtaaatccgc
     1741 gtaatcttca tcgattattt ctcgggctta tttattctca gtaacgcgaa tattcggggt
     1801 cgaggtatat atagagacgt cttatcttcc cgtcccttat ctcgaatacg tcgcaccact
     1861 tgctgtcagt ccagctctcg ccagtcttta gcttcccttc gctcgagcct tcggcgacga
     1921 ccatgtcgcc ctggattatc cagttaatgt acgcgtggtg gtgtatgaat tccgcgacac
     1981 gcgcgcccag gtcggagaaa agcctcttgt actcgaccgc ccctctcgca acgccccagt
     2041 tggggaagaa cacctcggcg tcttcagcga acaagttgaa gaagtccctc ccgtaatcaa
     2101 gactcttcag atattcggca gctatggatt ttctttcttc gtccgtcatt cgggcctcca
     2161 cggaatcgtt gataacaaac tttattataa atgatttgcc cgggcgagcc atctctggag
     2221 cgggtacata tccctgaaaa tatcgaagca gtaatctatg aagcggcggg agtatatatc
     2281 ttcgggaagg gcgctctcgc tgtatgcata aagaccgtta tgatgaagga gttccgcgtt
     2341 cgggtgctca gggtcgtaac ccctcggcac cctcttataa tgccggccgc ctattttata
     2401 ggacgggttc gcgactatcc cgtccaatat ttttttgagc tcagaccctc gcccgggatg
     2461 tactactgaa tccctgtatg acctgagcag atcgggaggg aaaatgtatt ctcccgcccc
     2521 tacgaaaatc gaggatttgt tgatctggac gtaatagttc gggttctcga gcttcttcct
     2581 cgggccgtcc cagaaaagta tgccgaggtg ggtcttgtag ggggtcttgt cggaggagaa
     2641 cctagtgtcc ctgtagatac ggaaaatcga tttatctatc aat
//
LOCUS       DASSJH010000349         6806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1052888, whole genome shotgun sequence.
ACCESSION   DASSJH010000349 DASSJH010000000
VERSION     DASSJH010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052888"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFJ67_07670"
     CDS             complement(<1..927)
                     /locus_tag="VFJ67_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HET7290302.1"
                     /translation="MQETITSLLTELSGIIWGLPLIMLLLGTGIYLTIILRGLQFRAL
                     IPSLYLAFVKRHEEGEGDISHFQALMTALSATVGVGNIAGVATAIAVGGPGAMFWMWV
                     TGFLGMATKYSEAVLAVKYREVDKLGTMSGGPMYYISKGLGLGWLGMLFAVFASLATF
                     GIGNMVQSNSVADAMQTAFGVPPWISGLVMCVATGLVIVGGIKSIAKATSAIVPFMLI
                     FYVIGASIILFEYRENIPNAFYLIFSHAFSPSAAAGGFLGSTLSQTVRIGVARGIFSN
                     ESGLGSSPIAAAAAKTKNPVGQALVSMTQTFID"
     gene            1167..2090
                     /locus_tag="VFJ67_07675"
     CDS             1167..2090
                     /locus_tag="VFJ67_07675"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7290303.1"
                     /translation="MDKLVIGHEEVKISLLLGIIAREHIYVEGPPGTAKTMLAEIISG
                     AAQLKFFFYQLHRDTRLSELIGDLVISKETTDTGEVIKQKVVKGGILTAEICLLDDIS
                     RAPGESLNVLLRILNERKFFDEVIPLLTAIATSNPTADEYYNEPLDPANLDRFVLQIN
                     AQGLSYGKRWEEAREVIKLYTQRPIEYHVPARVSREVFDEYYQKLGSLEIPLEVQEGL
                     ANFVATLIEDYGCNETNSLISDRTFFVKSLKIMRAHALLHGREVCTLEDLNALKYMTA
                     FRSPEEVFLQLDQIIEDLTSKKKKTGTGGAS"
     gene            2258..3238
                     /locus_tag="VFJ67_07680"
     CDS             2258..3238
                     /locus_tag="VFJ67_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET7290304.1"
                     /translation="MAPPDEMNIPSDPTGQASDDGRRRDDRQPPKTTKVFASKSPDPG
                     DEEDYWIGGKRNLESADNVKVIMKVLEGNFQRSIALRAPHPGGLPRKWKRMNYFDEME
                     DVDPYEAMFWTEYTTPTLPRVHKREKEMMGGEIAILRDVSTSMMGVYSEWSSSVVRGV
                     VELARSKKMRVGYVEFNHRSYKYKRNSKFFTRDYQWMLELASKTECSGNTNYEDALKD
                     ALTEFRGRGLRNKHILFITDGIPTSGDCDVLEERTRAKKLGVCIHSIFIGSKNYPRIL
                     QKISGETSGTQFIATRRKDGTIRIERRDKKVISPIPEDKAQVDPFSKVFA"
     gene            complement(3244..4245)
                     /gene="mltG"
                     /locus_tag="VFJ67_07685"
     CDS             complement(3244..4245)
                     /gene="mltG"
                     /locus_tag="VFJ67_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET7290305.1"
                     /translation="MGSGRIKTIFVAILLLLVLGASVLLSFYYYVTNIPAVSEDKVVD
                     IPKGRGLRGISAQLEAEGVIGNSNFFMLYVVGKGWQNRLKAGEYEFKRGSTTSEVAGR
                     IADGDVVLHKVTVPEGLTMKETAALLGKNGVLDPDGFLTAARDPALLKILPGRSITGL
                     EGYLFPDTYTYTKGVTPEEFVRMMLDRFNKVYRSLEGLRRRINLTDNEVVTLASIIEK
                     ETGAPAERPMISAVFHNRLRLGMKLESDPTVIYGMGEDFDGNLTKGDLNTMTQYNTYL
                     IKGLPPGPICNPGKESITAALSPAAVDYIYFVSRGDGTHYFSENYADHQRAVSEYQR"
     gene            complement(4255..5406)
                     /gene="proB"
                     /locus_tag="VFJ67_07690"
     CDS             complement(4255..5406)
                     /gene="proB"
                     /locus_tag="VFJ67_07690"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET7290306.1"
                     /translation="MKKSDRKSIISGVKRAIIKIGSSVLTDKKGNLSESVFDVLAGEI
                     SGINSRGIEIVIVSSGAIASGMKKLNLTSKPSEVSMKQAIAACGQSALIRNYERAFSS
                     FGLIVAQILLTHDGLADRKRFLNARKTLSTLLDMGIIPVVNENDTVAVEEIMLGDNDN
                     LAALVTSLVEADLLVLLTDIGGLYNKDPRKNEDAELISLIESIGKEIESVAGDTLGRT
                     TTGGMITKIQAARTAAAFGVPTIIANGKSGTALADIFDGKDVGTIFLPAKKILTGRKH
                     WIAFTLKSAGELVLDLGAVTAITAQGKSLLPSGIKDVRGKFGVGDSVTCVDESGVPVA
                     RGLTSYGSDDIRRIMGLSTKDVEKALGYKFTDEVIHRDDLAVIEKRSGR"
     gene            5499..5575
                     /locus_tag="VFJ67_07695"
     tRNA            5499..5575
                     /locus_tag="VFJ67_07695"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5533..5535,aa:Gly,seq:gcc)
     gene            complement(5581..6186)
                     /locus_tag="VFJ67_07700"
     CDS             complement(5581..6186)
                     /locus_tag="VFJ67_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7290307.1"
                     /translation="MLKLYDHPLSGNCYKVRLALNQLGVGYEKEYLDIFKGEQHSSGY
                     QSINPNKKIPVLIDDGFHMWESNAILLYIGRKYSPNRLYLEDLEGYGRMAQWLFFGKT
                     SIDPNLAVARYFTKFLSPENRDEKRLNELRTQGSAVLSVLDSHLEANDYLAGRYSMAD
                     IACYPYVNLAPEGGIDLSLYASVMLWLGRIRSQPGYISMED"
     gene            complement(6189..6680)
                     /locus_tag="VFJ67_07705"
     CDS             complement(6189..6680)
                     /locus_tag="VFJ67_07705"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7290308.1"
                     /translation="MIREIENIFLDFPGYGCFACDPRNTHGLRLRFFADDEKGDVYTR
                     TTPERHLQGFPGILHGGIQCALVDEVAFWTMFDRHKKIALTTNIEMEFLRPVGTASEI
                     EVRGRIVLEDGRRISVEVVIYDQEKVCTRSRVDYIIPKKEVTMKVMGKERFTGKFTKY
                     LDD"
BASE COUNT         1618 a         1909 c         1802 g         1477 t
ORIGIN      
        1 gtctatgaac gtctgggtca ttgagaccag ggcctggccc acggggtttt tggtcttcgc
       61 ggccgctgcg gctatgggcg agctccccaa acccgattcg tttgaaaata tgcccctcgc
      121 gaccccgatc ctgacggtct gactcagagt cgagcccagg aagccccctg cggccgccga
      181 aggggaaaag gcatgggaaa aaatgagata aaatgcgttt ggtatattct cacgatactc
      241 gaagagtatg atcgaggccc cgataacata gaatatgagc atgaacggta ctattgcgct
      301 cgtagccttc gctatgctct tgatcccgcc cacgatcacg agaccggtgg cgacgcacat
      361 gacgagaccg gatatccacg gaggaacgcc gaacgccgtc tgcatcgcat ccgcgaccga
      421 gttcgactgc accatgttcc ctatcccgaa tgtagctaac gacgcgaata cggcgaagag
      481 catgccgagc cagccgagcc ccagcccttt agaaatatag tacatggggc cgccgctcat
      541 ggtgccgagc ttgtcgactt ccctgtattt gactgcaaga acggcttcgg agtatttcgt
      601 agccatcccg aggaagcccg tgacccacat ccagaacatc gccccggggc cgcccacagc
      661 gatagctgta gcgacgccgg ctatgtttcc cacgcctacg gtcgccgata gcgccgtcat
      721 gagggcctgg aaatgtgaga tatcgccctc cccttcctcg tgtctcttta caaacgcgag
      781 gtaaagagaa ggaataagag cacggaactg gagccctctc aggataatgg tcagataaat
      841 gcccgtcccc aggagcagca taatgagcgg gagaccccat ataattccgc tcagctccgt
      901 cagcaacgat gttatggttt cctgcatgaa tgtcccttta aagaagaatt gtggctagaa
      961 acttatcata tttatccgat taaataaaac gcccgtattc gtgaacagcc ccttatcatt
     1021 ataaaaaccc ggcacgcccc gacagggagg cgtttattgc aaaagaaagg acgcttatgt
     1081 acaattattc catagctcaa ggaatattac ggaggcaata cgttataatg acatcacaga
     1141 atccatttat agctctgaaa gaagaaatgg ataaactggt tataggacac gaggaggtaa
     1201 agatttccct cctcctcggc ataatagcca gagagcacat ctatgtcgaa ggccctccgg
     1261 ggacggcgaa gacgatgctc gcggagatca tatccggggc ggcgcagctc aagtttttct
     1321 tctatcagct ccacagggac acgaggctat ccgagcttat cggcgacctt gtcatctcga
     1381 aggagaccac ggacacgggc gaggtaataa aacagaaggt cgtaaagggc ggcatactga
     1441 cggccgagat atgtcttctg gacgacatat ccagggctcc cggcgaatcg ctgaacgtac
     1501 tactgagaat actcaacgaa agaaaattct tcgacgaggt aataccgctt ctgaccgcga
     1561 tcgcgacgag caacccgacc gcggacgagt attacaacga gcccctcgac ccggcgaacc
     1621 tcgacaggtt cgtcctccag atcaacgccc agggcctgtc ctacggcaag agatgggagg
     1681 aggcgaggga ggtcataaag ctgtacacgc agagaccgat cgaataccat gtgcccgcaa
     1741 gggtcagcag ggaggtattc gacgagtact accagaagct cgggagcctc gaaataccgc
     1801 tcgaagtcca ggaagggctc gcgaacttcg tcgcgacgct catcgaggac tacggctgca
     1861 acgaaacgaa ttcactcata tccgacagga cgttcttcgt aaagtcgctc aagataatga
     1921 gggcgcacgc tcttctccac ggaagagagg tttgcacgct cgaagacctg aacgcactca
     1981 aatacatgac agcgttcagg agtccggaag aggtgttcct ccagctcgac cagattatcg
     2041 aggacctcac gtctaaaaaa aaaaagacag ggacaggggg agcgagctag aggaacagcc
     2101 gtccacggag ttcgataaag acagccctga agagaagaag caggatatcc ccgacgagca
     2161 gggcaagaaa gaatcgcagg accagctcac cgaagcgcgc aagacctttt tccaggcctt
     2221 aaaagaactt aaggaaaaca tccagaagga gagcagtgtg gctcctcctg acgagatgaa
     2281 tataccttcc gaccccacgg gccaggcttc cgacgacggc cgcaggaggg acgacaggca
     2341 gcccccgaag accaccaagg tattcgccag caaatccccc gatccgggag acgaggaaga
     2401 ttactggatc ggaggcaaga ggaacctcga atcggccgac aacgtgaagg tgataatgaa
     2461 agtcctcgaa gggaatttcc agaggagcat agcgctccgt gcgcctcatc cgggcggact
     2521 cccgaggaaa tggaagagga tgaattactt cgatgagatg gaagacgtcg atccgtacga
     2581 ggccatgttc tggacggaat acacgacgcc taccttgccc agggtgcata agcgcgaaaa
     2641 ggagatgatg ggcggggaga tcgccatact gagagacgtc agcacttcga tgatgggcgt
     2701 ttacagcgaa tggtcatcgt cggtcgtgag gggcgtcgtc gagctcgcga ggtcgaaaaa
     2761 gatgagggtc ggatacgtgg agttcaacca ccgctcgtac aagtacaaga gaaactccaa
     2821 attcttcacg agggactacc agtggatgct ggagctcgcc tccaagacgg aatgttccgg
     2881 gaacacgaat tacgaagacg cgctaaagga cgctctgacc gagttcaggg gccgcggcct
     2941 caggaacaag cacattctct tcatcacgga cggcatcccc acgtcgggcg actgcgacgt
     3001 gctcgaagag aggacgaggg ccaagaaact cggggtctgc atacactcga tattcatcgg
     3061 gtccaagaac tatccccgga tactccagaa gatttctggc gagaccagcg ggacgcagtt
     3121 catagccaca cgcagaaagg acggcacgat caggatcgag aggagagaca agaaggtgat
     3181 ctccccgatt cccgaagaca aggcgcaggt cgacccgttc agcaaggtat tcgcttagcc
     3241 gtttcacctc tggtattcgg acacggctct ctggtggtca gcgtaattct cggaaaagta
     3301 gtgcgtaccg tctccccttg atacgaagta tatgtagtcg acggcggccg ggctcagtgc
     3361 ggccgttatc gattccttcc cgggattgca tattggtccc ggcgggagcc ctttgatcag
     3421 ataagtattg tactgcgtca tcgtgttgag gtcgcctttg gtaaggttcc cgtcgaagtc
     3481 ctcccccatt ccgtagatca cagtagggtc gctctcgagc ttcatgccga gcctgagacg
     3541 gttgtggaaa acggccgaga tcatcggcct ctcggcaggc gcgccggtct ctttttctat
     3601 gatggacgca agtgttacaa cctcgttatc cgtgaggtta atcctccgcc tcaaaccctc
     3661 aagcgatcta tacactttat tgaaccggtc gagcatcata cgaacgaatt cctcgggcgt
     3721 gacgcctttt gtatacgtgt atgtgtccgg gaataaatag ccctcgagcc ccgtgatcga
     3781 gcgtccgggc agaatcttaa ggagcgccgg gtcccgggcc gcagtgagga aaccgtccgg
     3841 atcgaggaca ccgtttttgc cgaggagtgc ggcggtctct ttcatcgtaa gcccttcggg
     3901 gacagtaact ttgtgcagga ccacgtcgcc atctgcaatc ctcccggcta cttcagacgt
     3961 cgtacttccc ctcttgaact cgtactcccc cgctttgagc ctgttctgcc agcccttgcc
     4021 gacgacatag agcatgaaga aattactatt cccgatgacc ccctcagcct cgagctgtgc
     4081 cgatataccc ctcagacccc tgcccttcgg tatatccacc accttgtctt cggaaaccgc
     4141 gggaatgttg gttacatagt aataaaagct gaggagcact gatgccccca ggaccagcaa
     4201 taacagaatt gcgacaaata tggtctttat tcgtccggaa cccatatcgg gaactcacct
     4261 cccggatcgc ttttctatca cggcaaggtc gtcgcggtgt attacctcgt ccgtgaactt
     4321 atatccgagc gccttctcca cgtccttcgt gctaaggccc attatacgcc tgatatcatc
     4381 ggagccgtag gacgtgagcc ccctcgcgac gggtacgccg ctttcgtcga cgcacgtcac
     4441 cgagtcgcct accccgaact tcccccggac gtctttaatt cctgacggca ggaggctctt
     4501 cccctgtgcg gtaatggccg tcaccgctcc caagtccaga acgagctcgc ccgccgattt
     4561 aagcgtgaaa gctatccagt gcttcctccc tgtgaggatt ttcttcgccg gcaggaatat
     4621 cgtgcctacg tctttgccgt cgaaaatatc ggcgagagcc gtaccggact tgccgttcgc
     4681 gattatagtc ggcacgccga acgcggccgc cgtcctcgca gcctggattt tagttatcat
     4741 cccgcccgtg gtcgtcctac cgagggtgtc gcccgcgacc gactctattt ccttgccaat
     4801 actctcgatc aacgatatta attccgcgtc ctcgttcttt ctggggtcct tgttatagag
     4861 tcccccgata tccgtaagca ggacgagcag gtcggcctcc acgagcgatg tcacgagagc
     4921 cgcgagattg tcgttgtcgc cgagcattat ttcttcgacg gccaccgtat cgttctcgtt
     4981 cacgacagga attattccca tatcgaggag agtcgagagc gtcttccggg cgttcaggaa
     5041 ccttttcctg tcggcgagcc cgtcgtgagt gagcaggatc tgcgcgacga taagaccgaa
     5101 agaggaaaag gccctctcgt agttccgaat gagagcgctc tggccgcatg ccgcgatcgc
     5161 ctgcttcatc gatacctcgg acggtttaga ggtcaggttg agctttttca tgccggacgc
     5221 gatcgcgccc gaggaaacaa tcacaatctc gattccgcgc gaattaatcc cggaaatctc
     5281 acccgccagg acatcgaaca cgctctctga caggttgcct tttttatccg taaggacgct
     5341 gctgccgatt ttgataattg cccttttaac gccggatata atcgattttc tatcggattt
     5401 cttcatacga tcctgaaata atttattttt ctttcctcga ggtgttctcg agttcttgac
     5461 aacgaggccg agcctagcta tcttagttct tctacaaagc gggaatagct cagctggcta
     5521 gagcgtctcc ttgccaagga gaaggtcgcg ggttcgaatc ccgtttcccg ctccacccgt
     5581 ttaatcctcc attgaaatat aaccgggttg agatcttatt cggcctaacc acagcatgac
     5641 gctcgcgtac agcgaaaggt ctattccacc ctcgggggca agattcacat acggataaca
     5701 ggcgatatcg gccatggaat atctcccggc aagatagtcg ttagcctcca gatgagagtc
     5761 gagcacactt aggaccgcgc taccctgcgt gcggagctcg ttaagcctct tttcgtccct
     5821 gttctccgga gagaggaact tggtgaaata tctcgcgaca gcgaggttag ggtcaatcga
     5881 ggtcttgccg aagaacagcc actgcgccat cctgccgtaa ccttcgaggt cttcgagata
     5941 gagcctgttc ggcgaatatt tcctgccgat ataaaggaga atggcgtttg actcccacat
     6001 atggaaaccg tcgtctatga gcaccggaat cttcttgttc gggttaatcg actggtagcc
     6061 ggaagaatgc tgctctccct tgaagatgtc gaggtattct ttctcgtatc cgaccccgag
     6121 ctggttgagc gcgagtctca ccttgtagca attgccggat agggggtggt cgtaaagttt
     6181 gagcatagtc agtcgtccag gtatttagtg aattttccgg tgaatctttc ttttcccata
     6241 actttcatcg tcacttcttt tttcgggata atataatcga ccctgctcct agtgcaaacc
     6301 ttttcctgat cgtatataac cacttcgaca ctgatcctgc ggccgtcttc gagcacgatc
     6361 ctccctctca cctcgatctc ggaagccgtc ccgacaggtc tcaggaattc catttcgata
     6421 ttcgtggtga gcgctatctt cttgtgcctg tcgaacatgg tccagaaagc gacctcgtcc
     6481 acgagcgcgc actggatccc gccgtgcagt atcccgggaa agccctggag gtgccgctcg
     6541 ggcgtcgtgc gcgtatagac gtcgcctttt tcatcgtcgg cgaagaacct gagcctgagc
     6601 ccgtgcgtat tccgcgggtc gcaggcgaag cacccgtagc cgggaaaatc caggaatatg
     6661 ttttcaattt cgcggatcat ctgagcgctg aggatgtgcc gaagaaaaaa ttaccaagct
     6721 aatccctttc aaccacctta gcaacggcgg cgaccctctc gtcgggctcg agacctatga
     6781 gcctcactcc ctgggtccct ctcccg
//
LOCUS       DASSJH010000350         7419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_301508, whole genome shotgun sequence.
ACCESSION   DASSJH010000350 DASSJH010000000
VERSION     DASSJH010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301508"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFJ67_07710"
     CDS             complement(<1..407)
                     /locus_tag="VFJ67_07710"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HET7290309.1"
                     /translation="MNKKAVIILSGGIDSTTLVYSSIDQGYEVECLTILYGQRHSREA
                     DSARRITEALGVPHKVADLSPLGEILSGSALTDGRIDVPEVPETAEHYDTLKTTIVPN
                     RNAIFLSIALGYAVSTDADSVFFGAHSSDRGVYP"
     gene            complement(474..2654)
                     /locus_tag="VFJ67_07715"
     CDS             complement(474..2654)
                     /locus_tag="VFJ67_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c/FTR1 family iron permease"
                     /protein_id="HET7290310.1"
                     /translation="MIPFKRILALSVIFLFLFLHAQPSHSSDDPRRILSLVDYIGGDY
                     GNAVRDGQVINADEYHEMQEFSAGVEALFVTMKSAGGDKAGIEKDINTLNGLIRDKAP
                     ASEVKSLAGAIKGKIIDAYNIVPYPAKAPSYAAGKAVFENTCAQCHGAAGAGDGPLSQ
                     GLKPPPAVFTDPEVSLGLSPFKVFNTMTFGIEGTGMPSFPDLSDREKWDAAYYVLALG
                     YTERQVAEGKGFSAKLPGDIKDYKTLATLSNAELKERLDAISDSPDEETGALAYLREG
                     LLDRSAGGSPLLTAGSLLDESTSLYKQGKKEEAYTKALDAYLDGFEQAEADLRVRDKD
                     LTAAIEADFSDYRGAVKSGAPVEKIEELNSKIHGGLSSAERILNDETPSSNLLSFVNS
                     FSIMVREGLEAILIIAAIIAFMGATGARSQIRYIHYGWILALVAGVLTWLLARTVISI
                     SGAQREVIEGVTSITAAAVLFYVSYWLISKIEVRVWKQYIQGKVEKALSTGSVIALAS
                     VSFFAVYREAFETVLFYQALWFQSGNSQPAVLWGLAAGALVLVVLFYVLFKLALRIPL
                     KYFFSVTSVFLYLLSFILLGKGVKELQEAGIVGVTPVEYIPHIDILGLYPTLETSIPQ
                     ALLLAAFIFALVWLENVKREREKKEIAVSLSRIAEDMKSMHAAFDHIKGHIIEWRKCE
                     DIDLEAEDLDRRIQDVISHVDDLESKVGDFYTFVTKNNQSEKPH"
     gene            2892..4937
                     /locus_tag="VFJ67_07720"
     CDS             2892..4937
                     /locus_tag="VFJ67_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7290311.1"
                     /translation="MKEKINLTENLFEEFPEVTAAEWKAEIAKDLKGGDIGKLDWKPY
                     EGFTVSPFYTEDDLDTVGYLTEQYPGVFPYARGNETGGNDWKINEYITVGSVKEANRL
                     ALKSLAAGAQSLTFVCETGDGFISGVPVESARDMSALLKDIPIEEVPVHFKCGTGAGG
                     ILALYIIEAEKRGFDKKKLSGSIDADPLKSLALGGSFPGGERETFEELRSMISYLENN
                     MPYYRALEVSGYHFHDSGASAVQELAFTLASGVEYLDRLTSLDMSVDRITSHMSFSFS
                     IGSGYFMEIAKLRAARLLWASIVEAYGARKESAEKMSIKTRTSSRNKTVFDPYVNMLR
                     GTVEAMAAAIGGCESLNVLPLDAVYERPDEFTRRLARNTQLILKHESYLDRVTDPSGG
                     SYYIEKLTDSLADSAWELFGKIEGMGGLADALKAGFVQEEIQKTRNERDRDIASGRAI
                     LLGTNQYPDLGEKGPKKKGDVIPKKPLRAGKEKQSGTLSMKEYIDYLKGKKSYLGDIL
                     PDNPGAAGAGVEPLHPYRGGVPFEELRLAALKHRKETGVTPSVFPLPVGNPSMRNARA
                     AFTANFFGCAGFKITDNTGFNTADEGVKAALKSKSKIVVICSSDKEYPELAPVICAGL
                     KEKNPEIKVIIAGNPKEHADALKASGIDDFIHARSNMLQILRKYQEIYGIRPAGEEN"
     gene            4939..7089
                     /gene="scpA"
                     /locus_tag="VFJ67_07725"
     CDS             4939..7089
                     /gene="scpA"
                     /locus_tag="VFJ67_07725"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HET7290312.1"
                     /translation="MRPDFSRIDYKKRAQGKPGAGIIPPVSEWMTPEDISIHSAYTKE
                     DLLGIEHLRYAAGLPPYLRGPYPSMYVTRPWTIRQYAGFSTAEESNAFYRRNLAAGQK
                     GLSVAFDLPTHRGYDSDHERVVGDVGKAGVAIDSVLDMKILFNEIPLDDISVSMTMNG
                     AVLPVLAFYIVTAEEQGVAPEDLSGTIQNDILKEFMVRNTYIYPPGPSMRIVADIFKY
                     TAEHMPKFNSISVSGYHMEEAGASSDIELAYTLADGLEYVRTGINAGMNIDDFAPRIS
                     FFWGIGMNHFMEIAKMRAARMIWAKIIKRFGPKNPKSMALRTHCQTSGWSLTEQDPFN
                     NAVRTCVEALAAVLGWTQSLHTNALDEAIALPTDFSARIARNTQIYLEQETNICRSID
                     PWAGSYYVEYLTDRIAHRAWALIEEVESLGGMAKAIETGLPKMRIEEAAARKQARIDS
                     GQDTIVGVNKYVPDEEAEIDILEVDNTAVRSAQIERLKKLKKARNNDAVRAALDAITK
                     CAETGDGNLLGLSVEAARKRATLGEISDACEKVFGRYKAVIRTISGVYSGEMSEDSGF
                     RKARELADEFARLEGRRPRIMVAKMGQDGHDRGAKVIATSFADMGFDVDIGSLFQMPL
                     EAARQAVENDVHIIGISSLAAGHKTLVPELIKELKEMGRGDIMVVAGGVIPKQDYDFL
                     YKAGVTAVFGPGTVISKAAQEILGILIKKAGGKA"
     gene            7188..>7419
                     /locus_tag="VFJ67_07730"
     CDS             7188..>7419
                     /locus_tag="VFJ67_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-protein-alanine N-acetyltransferase"
                     /protein_id="HET7290313.1"
                     /translation="MENAPKYEIDRMTPRDLDGIVSIENVSFPTPWPRRVFERELKSQ
                     RSYNRVIRFGGMVVGYIVTWTIYDEVHILNIAV"
BASE COUNT         1781 a         2008 c         2148 g         1482 t
ORIGIN      
        1 gggtaaacgc ccctgtcgga gctgtgagcg ccgaagaaga cgctgtccgc gtccgtgctt
       61 acggcgtatc cgagcgcgat cgagagaaag atagcgttcc tgttgggcac tatggtcgtt
      121 ttgagcgtat cgtaatgctc cgctgtctcg gggacctccg gcacgtcgat cctcccgtcg
      181 gtcagcgcgg agcccgaaag tatttcgccg agcggggaaa ggtccgccac tttgtggggt
      241 actcccaggg cttccgttat cctgcgcgcg gaatcggctt ccctgctgtg cctctgtccg
      301 taaaggatag tgaggcattc gacctcgtac ccctggtcga tcgagctgta cacgagggtc
      361 gtgctgtcga tacctcccga caaaatgatg accgcctttt tattcatagc attaatacta
      421 tattcattag tatacaggga caagaaagta cgccgacgct tagggtggag aaatcagtgc
      481 ggcttttcgg actggttatt tttcgtgacg aaggtgtaga aatcgccgac cttgctttcg
      541 agatcatcta cgtggcttat gacgtcctgt attctcctgt cgaggtcttc ggcttcgagg
      601 tcgatgtcct cgcacttcct ccattcgatt atgtgcccct tgatatggtc gaaagcggcg
      661 tgcatcgact tcatgtcctc ggcgatgcgg gataagctca cggcgatctc cttcttctcc
      721 ctctccctct tcacgttttc gagccatacg agcgcgaaga tgaaagccgc caggagaagt
      781 gcctgaggga tagaggtttc gagggtggga taaaggccga gtatatcaat atgcgggatg
      841 tattcgaccg gtgtcacgcc cactatgcct gcctcctgga gctcttttac gcccttgccg
      901 agcaggatga aggaaaggag gtataggaat acgctcgtca ccgagaagaa gtacttgagg
      961 ggtattctca gggcgagctt gaataacacg tagaagagca ccacgagcac gagggccccg
     1021 gccgcgagac cccacagcac ggccggctgg gaattcccgg actggaacca gagggcctga
     1081 tagaagagga ctgtttcgaa agcctccctg tacacggcga agaacgatac gctcgcaagc
     1141 gcgataacgc tccccgtcga aagcgccttc tcgaccttcc cctgtatgta ctgcttccat
     1201 acgcggacct cgatcttgga gataagccag tagctcacat aaaacagcac ggccgccgcg
     1261 gtgatcgacg taacgccctc tatgacctct ctctgggctc cgcttatgga gatgaccgtt
     1321 ctcgccaaga gccacgtcaa tacgcccgca acgagagcca gtatccagcc atagtgtatg
     1381 tacctgatct ggctcctcgc tcccgtcgcg cccatgaacg ctatgatcgc cgcgattatc
     1441 agtatcgctt cgagtccctc tctcaccatg atcgaaaagg aattgacgaa ggagaggagg
     1501 ttgctggaag gggtttcgtc gttaaggatc cgctccgcgg acgataagcc gccgtgtatt
     1561 ttagaattga gctcttcaat cttttcaaca ggcgcgcccg acttgacggc gcccctgtag
     1621 tcagagaaat cggcctcgat cgcggccgtg aggtccttgt ccctgaccct gaggtcggcc
     1681 tccgcctgct cgaacccgtc gagataggcg tcgagcgcct tcgtgtacgc ctcttccttc
     1741 ttcccctgtt tgtatagcga cgttgattcg tcgagcagcg accccgccgt aaggagcgga
     1801 gagccgccgg cgcttctatc gagcaaacct tctcttaaat acgcgagcgc cccggtctct
     1861 tcgtccggac tatccgatat ggcatcgagt ctttctttca actctgcgtt cgagagcgtg
     1921 gcgagggtct tataatcctt tatgtctccg gggagctttg ccgaaaaccc tttcccctcc
     1981 gcgacctgcc gctccgtata gccgagagcg aggacgtagt aggcggcgtc ccacttctcc
     2041 cggtcggaaa gatcagggaa ggacggcatg cccgtccctt ctattccgaa cgtcatcgta
     2101 ttgaagacct tgaagggcga taagccgaga gagacctcgg ggtcggtaaa aactgcaggc
     2161 ggcggtttca gtccctggga taaggggccg tcccctgcgc ctgctgcgcc gtggcactgg
     2221 gcgcacgtat tttcgaatac cgccttccct gccgcataag aaggcgcttt tgccgggtag
     2281 gggacgatat tataggcgtc gatgatctta ccctttatag cccccgcgag cgatttcact
     2341 tcgcttgccg gagctttgtc ccggatgagg ccgttgagtg tgttaatgtc cttttctatc
     2401 cccgctttat cgccgccggc cgatttcatt gtaacgaaca gggcctcgac gccggccgag
     2461 aactcttgca tctcgtggta ctcgtccgcg tttattacct gcccgtccct gaccgcattc
     2521 ccgtaatccc ctcctatgta atcgacgagg gacaggatac gtctcgggtc atccgaagaa
     2581 tgagacggct gggcgtgaag aaacaggaat aaaaaaataa cggaaagcgc taaaatcctt
     2641 ttgaacggga tcataatttc tgttgcggaa gccccctgtc gtattcgcgg ataactaatt
     2701 tgaagtatac agcaaccacc cgaatagaaa atgaatatta atatcaacta acaaaccatt
     2761 tgagggtatc acgagccatt atcatgtcaa gacacaaagt tgcatgatac cgtgttttat
     2821 ttattattat tgagttattt tatacgggtt cgtaaaaatt cgaccgggtg cgggacgccg
     2881 caaatcaccg aatgaaagaa aagataaatc tcacggaaaa tctgtttgag gagtttccgg
     2941 aagtaacggc cgccgagtgg aaagcggaga tcgcgaagga cctcaaaggc ggggacatag
     3001 gcaagctcga ctggaagcct tacgagggct tcaccgtaag cccgttctat accgaagacg
     3061 atctcgatac cgtcggatac ctgacggagc agtatccggg agtgttcccg tatgcccgcg
     3121 gcaacgagac gggcggcaac gactggaaga tcaacgaata tataaccgtg ggcagcgtta
     3181 aagaggctaa caggctcgcg cttaaaagcc tcgccgcagg cgcgcagtcc ctcacattcg
     3241 tctgcgagac aggggacgga ttcatatccg gcgtccccgt cgagagcgcc cgcgatatgt
     3301 cggcgttgtt aaaagacata ccgattgaag aggtgcctgt acatttcaag tgcggaactg
     3361 gggccggagg tatattagcg ctctacatca ttgaagccga aaaaaggggc ttcgacaaga
     3421 aaaagctctc ggggtctatc gacgccgatc cgctcaagtc actcgcccta ggcggctcat
     3481 tcccgggagg cgagcgcgag acgttcgaag agctgcgctc gatgatctcc tatctcgaaa
     3541 acaacatgcc gtattaccgc gcgctcgaag tatccggcta tcacttccac gattcgggcg
     3601 cttcggccgt gcaggagctc gccttcacgc tcgcctcggg cgtcgaatat ctggaccgcc
     3661 tcacgtcgct cgatatgagc gtagaccgta ttacctctca catgagcttc tcgttctcca
     3721 taggctccgg atacttcatg gaaatagcga agctccgcgc agcaaggctc ctgtgggcgt
     3781 ctatcgtgga agcgtacggc gcccggaaag agtcagccga aaaaatgagc atcaaaacca
     3841 gaacgtcctc gcggaacaag accgtcttcg acccttatgt caatatgctg agggggactg
     3901 tggaggccat ggccgcggct atcggagggt gcgagtctct caacgtgctt cctctcgatg
     3961 ccgtttacga gagacccgac gaattcacgc gcaggctcgc ccggaacact cagctcatac
     4021 tcaagcacga atcctacctc gaccgcgtga cagacccctc gggaggttct tattatatcg
     4081 agaagctcac ggactctctc gccgactcgg catgggagct gttcgggaag atagagggaa
     4141 tgggcgggct ggccgatgcg cttaaggccg gattcgtaca ggaagagata caaaaaacca
     4201 ggaatgaaag agaccgcgac atagcgtccg gaagggcgat actcctgggc acgaaccagt
     4261 accccgatct cggtgaaaag ggtccgaaaa agaaaggcga tgttattccg aaaaaaccgc
     4321 tcagggcggg gaaggagaag cagtcaggca cgttgtcaat gaaagagtac atagactacc
     4381 tcaaaggaaa aaaatcatac ctcggggaca tactgccgga taatcccgga gcggccggcg
     4441 cgggagttga accgcttcac ccttacaggg gcggagtgcc gttcgaggag ctgaggctcg
     4501 ccgcactgaa acacaggaag gaaacaggcg ttacgccctc ggtcttcccg ctccctgtcg
     4561 ggaacccgtc gatgcggaac gcgagggcgg cgttcacggc gaatttcttc gggtgcgcag
     4621 ggttcaaaat aactgacaat acaggcttca acacggccga cgaaggagtg aaggccgctc
     4681 tcaaaagcaa gtcgaagata gtcgtcatct gcagctcgga caaggaatac ccggagcttg
     4741 cgcccgtgat ctgcgccggg cttaaagaga agaatcctga aataaaggtt atcatcgccg
     4801 gcaatccgaa ggagcacgcc gacgccttga aagcgtccgg aatagacgac ttcattcacg
     4861 cccgttcgaa tatgctacaa atcctgagaa aatatcagga gatttacggt ataagacccg
     4921 caggcgagga aaactgaatt gagacccgat ttttcgagga tcgattacaa aaagagagcg
     4981 caggggaaac ccggagccgg gattattccg ccggtttcgg aatggatgac gcccgaggac
     5041 atatccatcc attcagcata tacgaaggaa gaccttctcg gcatcgagca cctccgttac
     5101 gcagcgggcc tgccccccta cctgagaggc ccttatccgt ccatgtacgt gacacggccg
     5161 tggacgataa ggcagtacgc cggtttctcg accgccgagg aatcgaacgc gttttacaga
     5221 cggaatctgg ccgcggggca gaaaggcctc tccgtggcgt tcgacctgcc cactcacagg
     5281 ggctacgact cagatcacga gcgtgtcgtg ggcgacgtcg gtaaggcggg tgtagcgata
     5341 gattcagtcc tcgacatgaa gatactgttt aacgagatac cgctcgacga catctcggtt
     5401 tcaatgacaa tgaacggagc ggtgctgcct gtgctcgcgt tctacatcgt aacggccgag
     5461 gagcagggtg tagcccccga ggacctgagc ggcacgattc agaacgacat actcaaggaa
     5521 ttcatggtga ggaacacgta catctacccg cccggaccgt ccatgaggat cgttgccgac
     5581 atattcaaat acacggccga gcacatgccg aaattcaact ccataagcgt gagcggatac
     5641 cacatggaag aggccggggc ttcctcggac atagagctcg cgtacaccct cgcagacggg
     5701 ctcgagtacg tgaggaccgg gataaacgcc ggaatgaaca tcgacgattt cgccccgagg
     5761 atctcgttct tctggggaat cggtatgaac cattttatgg agatagccaa gatgagggcg
     5821 gcgcgtatga tctgggccaa aataataaag aggttcggcc ctaagaaccc gaagtcgatg
     5881 gccttgagga cgcactgcca gacctcgggc tggagcttga ccgagcagga cccgttcaat
     5941 aacgccgtga ggacgtgcgt cgaggcgctc gccgccgtgc tcggctggac gcagtcgcta
     6001 catacgaacg cgctcgacga agcgatagcg ctgccgacgg atttctcggc gcgcatagcc
     6061 cgtaacacgc agatatacct cgagcaggaa accaatattt gcaggtctat tgacccttgg
     6121 gcgggctctt attatgttga atacctgacg gacaggatag cgcaccgcgc ctgggcgctt
     6181 atagaggaag tcgagagcct gggcggcatg gcgaaggcga tcgagaccgg cctccccaag
     6241 atgaggatag aggaagcggc cgcgaggaag caggcgagga tcgattcggg acaggacacg
     6301 atagtcgggg tgaacaaata cgtgcccgac gaagaagccg agatcgatat actcgaagtc
     6361 gataacaccg ccgtgaggtc ggcacagata gagcgtttga agaagctcaa aaaggcgcgt
     6421 aacaacgacg ccgtgagagc cgccctcgac gcgatcacga agtgcgcgga gacgggcgat
     6481 gggaacctcc tcgggctcag cgtggaagcg gcccgcaagc gcgcgacgct cggggagata
     6541 tcggacgcgt gcgagaaggt gttcggacgc tacaaggcgg tgatacgcac gatatcgggg
     6601 gtttattcgg gagaaatgtc agaggacagc gggttcagaa aagcgaggga acttgcggac
     6661 gagttcgccc ggctcgaggg caggaggccg cgtataatgg tcgcgaagat ggggcaggac
     6721 ggccacgaca ggggagccaa ggtcatcgcc accagcttcg ccgacatggg gttcgacgtg
     6781 gacatagggt cgctcttcca gatgccgctc gaagcggcga ggcaggcggt agagaacgac
     6841 gtgcatataa tcggaatatc gagcctcgcc gcgggccaca agacgctcgt tcccgagctc
     6901 ataaaggaat taaaggagat ggggcgggga gacatcatgg tcgtcgccgg cggagtcata
     6961 cccaaacagg attacgattt cctttacaaa gcgggggtca cggccgtatt cgggccgggc
     7021 acggtcatct cgaaggcggc tcaggaaatt ctcggcatac ttatcaaaaa agccggggga
     7081 aaggcctgac agccgagaag gagccgggga cagttcctat gagaaaaaat cctttgattc
     7141 cgcacactga atacttcagg aacattttcc aagccggata gaataaaatg gaaaacgcgc
     7201 ccaaatacga gattgacagg atgacgcccc gggacttaga cggcatcgta tccatcgaga
     7261 acgtaagttt cccgaccccg tggccgaggc gcgttttcga gcgcgagctg aaatcgcaaa
     7321 ggtcatataa cagggttata cggttcggcg gaatggtcgt cggttacatc gtcacatgga
     7381 ccatatacga cgaagtccac atcctgaaca tagccgtgc
//
LOCUS       DASSJH010000351         9733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_518059, whole genome shotgun sequence.
ACCESSION   DASSJH010000351 DASSJH010000000
VERSION     DASSJH010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518059"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..344)
                     /locus_tag="VFJ67_07735"
     CDS             complement(78..344)
                     /locus_tag="VFJ67_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290314.1"
                     /translation="MSVYLITYELHHDKDYQKIQEAIESLGGCIHCLRSIWLVNSAFS
                     IAEIHEILKDKIDSDDSLLILKPSREWDSFNLPIASKTWLKHHL"
     gene            complement(513..3531)
                     /locus_tag="VFJ67_07740"
     rRNA            complement(513..3531)
                     /locus_tag="VFJ67_07740"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3638..3711)
                     /locus_tag="VFJ67_07745"
     tRNA            complement(3638..3711)
                     /locus_tag="VFJ67_07745"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3675..3677),aa:Ala,seq:tgc)
     gene            complement(3774..3850)
                     /locus_tag="VFJ67_07750"
     tRNA            complement(3774..3850)
                     /locus_tag="VFJ67_07750"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3814..3816),aa:Ile,seq:gat)
     gene            complement(3959..5521)
                     /locus_tag="VFJ67_07755"
     rRNA            complement(3959..5521)
                     /locus_tag="VFJ67_07755"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            5920..7071
                     /gene="moeB"
                     /locus_tag="VFJ67_07760"
     CDS             5920..7071
                     /gene="moeB"
                     /locus_tag="VFJ67_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET7290315.1"
                     /translation="MGLTNEQIRRYSRHLIMPEVGLEGQEKLVNSRVLCVGAGGLGSP
                     LALYLAAAGVGHMGILDFDVVDFSNLQRQIIHSEETLGVLKVESARDRLLELNSDIDV
                     TTYNTMLTSENAMDIIKDYDIVIDGTDNFATRYLVNDACVLLGKPNVYGSIFRFEGQV
                     SVFDAKRGPCYRCLYPEPPPPGLVPSCAEGGVLGVLPGIVGTLQAAEAIKLIIGKGVP
                     LIGKLLFIDVMEMKIRELKLRKDPDCPICGEHPIIKELIDYQEFCGIGRGEEVEGFAE
                     SDSLEISVDDFYKLRTNNGKIVILDVREPHEFEICNFGDSKLIPLGELPERVNELDTA
                     DDIVTVCHVGGRSLQATRLLRNMGFKKVKNLKGGIDAWAEKYDPEMPRY"
     gene            7184..8305
                     /locus_tag="VFJ67_07765"
     CDS             7184..8305
                     /locus_tag="VFJ67_07765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7290316.1"
                     /translation="MMKRIRTAHILLALLSIIIALALAETGIRTIERLGYFPYFFKAL
                     NSAYTPFDVKTGEGLYYSHPYISYDMKPGYNIPGDVTINSLGFRGEEFDAVKPPGVYR
                     IVALGGSTTYGIGLNDNETYPYYLQKELRKRLGTYKVEVINAGLVSATSAESLSRFLF
                     KVLPLDPDMVIFYEGYNDLPPRMFNGYSDDYYNFRKNPQNHFPWLSHSLLYRLAASGF
                     KASLHYPNMTLLSEIWKFENLPQEDDKKIANFNSTSSAVYERNLDYLIVLAGAKNITV
                     VLSTFVFDDDAPDWNDYMPGELWGKGIGENNEAIRELAGKYRLPLIDLYSYGLGNKHI
                     FKDSIHLTAEGNEEIADVFADTIAPIIKEEMGNKFELAK"
     gene            complement(8419..9348)
                     /locus_tag="VFJ67_07770"
     CDS             complement(8419..9348)
                     /locus_tag="VFJ67_07770"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HET7290317.1"
                     /translation="MKTSRDIVKHLYNEKLAEKPRTFTEKLRWKMKYDRRPILTLTAD
                     KYRVIDYLKQNGFGNLLKKLYFVTDNPAEIPFDDLPERYIIKSSHSSGDIMIIDGGFD
                     LVTKDRVGRWELIVKCNQFLRRRHWNELNEWAYQGIKPVIIVEEFLSDENGLPPVDFK
                     FLCFDGKASIVEVIEDRYGDYTDNYFDMNWQPLDFTWSDWLGITGGPPGDNVQRPANF
                     GEMIRIAGELSKLFDFVRVDLYNVMGKIYFGEFTHYPSGGIGEIEPRSFDAWMGSLWT
                     LPDIDTVKDYSFLSRINEAYYSLYYRYNFWRYK"
     gene            9529..>9733
                     /locus_tag="VFJ67_07775"
     CDS             9529..>9733
                     /locus_tag="VFJ67_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008554580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290318.1"
                     /translation="MKVNNSADTTLPVIQSMWIGSELSVMEQLSIISFLSNGHSYHLY
                     TYNEVKNVPEGVTLKDANGIITPD"
BASE COUNT         2348 a         2705 c         2269 g         2411 t
ORIGIN      
        1 taattaaagt caacagactg acgtcattgt aacccgaaat atcaatcatc aggatattta
       61 gtgaattgat attagagtta caaatgatgc ttgagccagg ttttgctggc gatcgggagg
      121 ttaaagctgt cccactcgcg ggagggcttt aatataagga gggaatcgtc tgagtcgatc
      181 ttgtccttta gtatctcgtg gatctccgcg atggagaaag cgctattcac gagccatatc
      241 gagcggaggc agtgtatgca tccgcccagc gactctatcg cctcctgtat cttttgatag
      301 tccttatcgt gatggagctc gtacgtgatg agatatacgg acatcgggac acccccctct
      361 agagaataaa ataatccatg taatcagatt acaggaatta tttatgttag ggaatatcct
      421 gacggattat agaagatgca aacaaaagat cttcgacgat ttcatattgt gctgagttct
      481 cgcgtgaaat tagcggtaac tttcaaaacc ccgcagttaa gccgcacggt cagttagtac
      541 acttcagctc aacacattac tgtgcttaca cctagtgcct atcaacctcg tagtcttcga
      601 gggaccttta gaaatcttaa gatttgggag acctagtctt ggggtgggtt tcccgcttag
      661 atgccttcag cggttatccc gtccgagcat aactacccag cgctgccact ggcgtgacaa
      721 ctggtacact agaggctcgt ccactcaggt cctctcgtac tatgagcaga tcccctcaag
      781 tctcctacgc ccgctgcaga tagggaccga actgtctcac gacgttctaa acccagctcg
      841 cgtaccgctt taattggcga acagccaaac ccttgggacc ttctccagcc ccaggatgcg
      901 atgagccgac atcgaggtac caaacctccc cgtcgatgtg gactcttggg ggagataagt
      961 ctgttatccc cggcgtacct tttatccgtt gatcgatggc ccttccactc ggaaccaccg
     1021 ggtcactaag acctgctttc gcatctgctc gacttgtcag tctcgcagtc aagctccctt
     1081 atgcctttgc actcttcgcc tggtttccaa tcaggctgag ggaacctttg cacgcctccg
     1141 ttactcttta ggaggcgacc gccccagtca aactacccgc ctaacattgt cctggatccg
     1201 gatgacggat ctcagttaga atcctaatac tccaagggtg gtatttcact aatgcctccg
     1261 ccctgcccaa aagcagagtt tcacaggctc ccacctatgc tacgcatgca gcaccaaaat
     1321 ccagtgctaa ggtatagtaa aggtgcacgg ggtctttccg tcccgcagcg ggtacccggc
     1381 gtcttcaccg gaaccacaat ttcgctgagc atttggccga gacagcgcgg caatcgttac
     1441 gcctttcatg caggtcggaa cttacccgac aaggaatttc gctaccttag gaccgttata
     1501 gttacggccg ccgtttaccg gggcttcggt tctgagcttc tcctggcgaa ccagaataac
     1561 ccatcccctt aaccgtccgg caccgggcag gcgtcagacc ctatacgtcg tcttgcgact
     1621 ttgcagagcc ctgtgttttt agtaaacagt cgtcaccgcc tctttactgt gacccacttc
     1681 ggctccagcc gcagggcttt caccttatgt gggcacccct tatcccgaag ttacggggct
     1741 atgttgccga gttccttagc caaagttctc tcacgcgcct tagtatactc aactcgccta
     1801 cgtgtgtccg tttacggtac gggcactcaa tcatcaacgc tacgaggctt ttctcgaaag
     1861 tttgggctca atcgctttgt ggggctaacg cccctcgtac tcgctcctca gggttaatga
     1921 agagacggat ttgcctatct cttcccccta caagcttgaa ccgggcaatc caatccccgg
     1981 ccgacctacc ctcctttgtc accacttcac tccgattgag tggtacggga atattaaccc
     2041 gttttccatc gactacgcct ttcggcctcg ccttaggtcc cgacttaccc tgggaggacg
     2101 agccttcccc aggaaccctt aggcttacgg cgaggtggat tctcaccacc tttatcgcta
     2161 ctcatgccag cattctcact tctcaacagt ccaggcctcc ttacggtaac ccttcagccc
     2221 attgagaacg ctcccctacc gctgtataaa tacaacccgt agcttcggca ccgggcttga
     2281 gccccgtttc attttcggcg cgaagtcgca tcgaccagtg ggcagttacg cactctttaa
     2341 agggtggctg cttctaagcc tacctcctgg ctgtctatgc gacttcacat cctttaccac
     2401 ttagcccgaa ttttggggcc ttagctgacg atctggggtg tttccctctc gcccaaggaa
     2461 cttatctccc ttggactgac tcccacacta agcaagatgg cattcggagt ttgattgggt
     2521 ttggtaggtc tttcaacccc ctagcccatt cagtgctcta cctccacccg ccaacatgtg
     2581 aggctatacc taaatatatt tcggggagaa ccagctatca cgagccttgt ttagtctttc
     2641 gctccgatcc acaactcatc cgataggttt tcaaccctaa ccggtgcggg cctccacgga
     2701 ggttttctct ctgcttcacc ctggtcatgg atagatcgac tcgcttcggg tctatctcca
     2761 gcgactgaac gccccgttca gactcgcttt cgctacggct acacctttct cggcttaacc
     2821 ttgccactga agataactcg ctggcccatt atgcaaaagg tacgcggtca ggcatttcct
     2881 ggaagcaagc tcccgggata tagcccttcc accgatcgca ggcagacagt ttcagattct
     2941 atttcactcc cctcaacggg gttcttttca cctttccctc acggtacttg ttcactatcg
     3001 gtcgacggca cgtatttagc cttggaaggt ggtcctccca gattccccca aactttcacg
     3061 tggtccgggg tactcgggaa tacagcaagg aagactttaa tgctttcgtt tacaggactg
     3121 tcaccctcta tggtgcaact ttccagatgc ttcaactagc ataaagtttt gtaacttccc
     3181 gaaggattta cggatccttc attccgcatc ccacgacacc ttatatgcaa cgcccgtaag
     3241 ctttaacacg tataaggttt aggctcttcc cctttcgctc gccgctacta agggaatatc
     3301 ggttgatttc tcttcctgag ggtactgaga tgtttcactt cccctcgttt gcttattagg
     3361 gctatgtatt caccctaata tgaccgaggt ttgctcggcc aggttttccc attcggaaat
     3421 ccacggatca cagcctgttt agcggctccc cgaggcttat cgcagcttac tacgtccttc
     3481 atcgccgtcc gtcgccaagg catccacata tctgccctga gtatcttaac tgcaaggtat
     3541 gaaaatcacc gcataattaa gcacgctcga ttactcagca caatatgaaa ttgtcaaaga
     3601 tccatgcaaa taaacatttg ctttgtttgc aattgtatgg aggtgagcgg gatcgaaccg
     3661 ccgacctcct gcttgcaagg caggcgctct cccaagctga gctacacccc cggtctaact
     3721 actaatccga gaatctcaat caattaagaa tcaggtatta gttcaactat cggtggtggg
     3781 cctaggtgga gtcgaaccac cgacctcacc cttatcaggg gtgcgctcta gcctgctgag
     3841 ctataggccc gtcgttagtt gacctaacag ccgactccac ttttactccg ggaaagcacc
     3901 aaaatttaat agcagataaa gcgaacaaaa gaaagagaaa gcaatttttc tccgtgaaaa
     3961 aggaggtgat ccagccgcag gttcccctac ggctaccttg ttacgacttc accccaatta
     4021 ccagccctac cttaggagcc tgcccccctt gcgggttagc tcagccactt cgggtagaac
     4081 cgactttcgt ggtgtgacgg gcggtgtgtg caaggcccgg gaacgtattc accgtggcgt
     4141 tgctgatcca cgattactag cgattccaac ttcatgcagg cgagttgcag cctacaatcc
     4201 gaactgagac tgactttgat gggattggct tcgcctcacg gttttgcaac ccattgtatc
     4261 agccattgta gcatgtgtgt agcccaggac ataagggcca tgatgacttg acgtcgtccc
     4321 caccttcctc cggcttgtcg ccggcagtct ctttagagtt cccaccttaa gtgctggcaa
     4381 ctaaacatag gggttgcgct cgttaccgga cttaaccgga cacctcacgg cacgagctga
     4441 cgacagccat gcagcacctg tactaactgt gtgaagcaag ctccacacca ctccatgctt
     4501 tcacataagc ttgagtcagt atgtcaagcc ctggtaaggt tcttcgctta gcatcgaatt
     4561 aaaccacatg ctccaccgct tgtgcgggcc cccgccaatt cctttgagtt tcaatcttgc
     4621 gaccgtactc cccaggcggg atgcttaatg cgttaacttc ggcacagaga ttttcatccc
     4681 cacacctagc atccaacgtt tagggcgtgg actacccggg tatctaatcc ggttcgctac
     4741 ccacgctttc gtccctgagt gtcagtaccg ggccagttgg ccgccttcgc cactggtgtt
     4801 cctccagata tctacgcatt tcaccgctac acctggaatt ccgccaacct ctcctggact
     4861 caagccttgc agtattagat gcagtttcag ggttaagccc taagatttca catctaactt
     4921 acgaggccac ctgcggaccc tttacgccca gtaattccga gcaacgcttg ccccctccgt
     4981 attaccgcgg ctgctggcac ggagttagcc ggggcttcct ttgtaagtac cgtcaagtct
     5041 ccgggatatt agcccggaga cccatcgttc ctactgacag aggtttacga cccgaaggcc
     5101 ttcatccctc acgcggtatt gctgggtcag gcttgcgccc attgcccaag attccccact
     5161 gctgcctccc gtaggagtcg gggccgtgtc tcagtccccg tgtggccgat cacccgctaa
     5221 ggtcggctac ccgtcttcgg cttggtgagc cgttacctca ccaactacct gatgggacgc
     5281 ggactcatct ttcggtgaca gcccgaaggc cgccttttac ccctggttcc atggaacctg
     5341 tggtcttatc cggtattagc cctagtttcc cagagttatc ccagtcctaa aggcagatta
     5401 tccacgtgtt actcacccgt ccgccgctct actcatgacc gaagtcactt tcgcgctcga
     5461 cttgcatgtg ttaagcatac cgccagcgtt cgctctgagc caggatcaaa ctcttcatat
     5521 aatcttatga aaggattgct tagctcttct tcgcccgctt atctgctatt taattttcaa
     5581 agatctgaat atcttttcag atattttgcc aaagggacat ataatataaa acaacgaaaa
     5641 tggatgtcaa gacaatttga cattcttttg taaaaatctt taaaatccga aaaaccggcc
     5701 cgatcccgct cccttcggag ctgaatccat cagaatacgg aatgtatcta acttgtttaa
     5761 accggactca gaccttaata tattatacat gattatttga agtgcaagga gcccttgttg
     5821 cgcagccggc gccccggccc cggcgggttg aaagtatagg gattctgtat tactttatat
     5881 ccctcacaaa aattataaaa taaagggacg gttgaaatta tgggtcttac aaacgagcaa
     5941 ataagacgtt actcaagaca cctgataatg cccgaagtgg gcctggaagg ccaggaaaag
     6001 cttgtcaatt ccagggtgct ctgcgtgggc gcaggagggc tgggctctcc gctcgcgctc
     6061 tacctcgccg cagcgggcgt agggcacatg ggcatactcg acttcgacgt ggtcgatttc
     6121 agcaacctcc agaggcagat catacacagc gaagagaccc tcggcgttct caaagttgaa
     6181 tccgcccgcg acaggctcct cgagctcaac tccgacatcg acgtcacgac ctacaacacc
     6241 atgctcacgt ctgagaacgc gatggacata ataaaggact acgacatcgt tatagacggc
     6301 acagacaact tcgccacgag atacctcgta aacgacgcgt gcgtgctgct cggcaagccc
     6361 aacgtgtacg gaagcatatt ccggttcgag ggacaggtga gcgtattcga tgcgaaaagg
     6421 ggcccttgtt acaggtgtct ttatccggag cccccgccgc cggggctcgt gccgagctgc
     6481 gccgaggggg gagtgctcgg agtactgccg ggcatagtcg gaacactcca ggccgcggaa
     6541 gctataaagc tcataatcgg caagggagta ccgctcatag ggaagctcct gttcatcgac
     6601 gttatggaga tgaagataag ggagctcaag ctccgtaagg atcccgactg cccgatatgc
     6661 ggcgagcatc cgataatcaa ggagctcata gactatcagg agttctgcgg catagggcgc
     6721 ggcgaagagg ttgagggttt cgccgaatcg gacagcctcg agatctcggt cgatgatttc
     6781 tacaagctcc gcacgaacaa cgggaagata gtcatcctcg acgtgaggga gccgcacgag
     6841 ttcgagattt gcaacttcgg cgactcgaag ctcatcccgc tcggagagct cccggaaagg
     6901 gtgaacgagc tcgatacggc ggatgatatc gtcacggtct gccacgttgg gggaaggagc
     6961 ctgcaggcga cgaggcttct ccggaatatg ggcttcaaga aggtcaagaa cctgaaaggc
     7021 ggcatagacg cctgggccga gaaatacgac ccggagatgc cgaggtactg atacgtccgt
     7081 atgtcgtcag gaacgtgtgc gtcgaacacc gcgggcgcga tcgaacatgc agccgaggga
     7141 gttttcaact ctttaggctc agcatatcag caccggaaac ctgatgatga agcgtatcag
     7201 gacagcacac atactcttag cccttctttc aattataatt gccctcgcct tggccgaaac
     7261 cggcatccgt acaatcgagc gcctgggtta cttcccgtat ttcttcaaag ctctcaacag
     7321 cgcttatacg cccttcgacg ttaaaaccgg cgagggcttg tactattccc acccatatat
     7381 cagttacgac atgaaacccg gctacaacat acccggcgac gtcacgataa attctctcgg
     7441 attcagagga gaggagttcg atgccgttaa acccccgggg gtttacagga tcgtcgctct
     7501 cggaggctcg acgacatacg gcataggact caatgataac gagacctacc catattatct
     7561 ccagaaagaa ctgaggaagc gcctgggcac ttataaagtc gaagtaatca atgcagggct
     7621 cgtcagcgcg acctcggcgg agagcctctc gcggtttctg tttaaagtac tcccgctcga
     7681 cccggacatg gtgatcttct atgaaggcta caacgacctc ccgccgagga tgttcaacgg
     7741 ctactcggac gactattata acttcagaaa aaaccctcag aaccactttc catggctttc
     7801 gcattcgctc ctctacaggc tggccgcttc gggtttcaag gcgagcctcc attaccctaa
     7861 tatgacgctt ctttcggaga tctggaaatt cgaaaacctc ccgcaggaag acgataaaaa
     7921 aatcgccaat ttcaactcca cgtcgagcgc cgtttatgaa agaaacctgg attatttaat
     7981 cgtgcttgcc ggagcgaaga acatcaccgt cgtgctctcg actttcgtat tcgacgacga
     8041 cgctcccgac tggaacgact acatgcccgg cgagctatgg ggaaagggga tcggtgaaaa
     8101 caacgaggcg ataagagagc tggccgggaa atacaggctg cctctcatcg acctctattc
     8161 gtacggactc gggaacaagc acatattcaa ggattcgatc catctgaccg cggaagggaa
     8221 cgaggaaata gcggacgtgt tcgccgatac gatcgcgccg atcataaagg aagaaatggg
     8281 caataaattt gagctagcaa aataattaca acccgccact cagccgtaag cgaaacacgg
     8341 cattcgtagc gctggaaatc acagaaacaa gtgaaggaca tcaggttccc gggcagggcc
     8401 ggacgcggaa gggaaaagtt atttatatct ccagaagttg tatctgtaat agagactgta
     8461 ataagcctca ttgatacggc ttaaaaacga atagtccttc acggtatcga tatccgggag
     8521 agtccagaga cttcccatcc aggcatcgaa cgagcgcggc tcgatctcgc cgataccgcc
     8581 cgaggggtaa tgagtaaact cgccgaaata aattttcccc ataacattgt ataaatccac
     8641 tcgcacgaaa tcgaacagct tgcttagttc cccggcaatc cttatcatct caccgaaatt
     8701 ggccgggcgt tgaacattgt ccccgggagg acctccggtt atcccaagcc aatcggacca
     8761 tgtgaagtcc aacggctgcc agttcatgtc gaaatagttg tccgtgtaat cgccatatct
     8821 gtcctctatg acctcgacta ttgaagcctt gccgtcgaag cacaagaact taaagtcgac
     8881 cggtggtaat ccattctcgt cggaaagaaa ctcctcaaca attatcacag gttttattcc
     8941 ctggtacgcc cattcgttaa gctcattcca gtgccggcgt cgtaaaaact gattgcattt
     9001 cactatcagc tcccatctac cgacgcgatc cttggttact aaatcgaagc cgccgtctat
     9061 tatcataata tcgcccgatg aatgactcga cttaatgatg tagcgctcgg gaagatcgtc
     9121 aaatggtatt tcggctggat tatccgttac gaaataaagc tttttcaaga ggttgccaaa
     9181 tccgttttgt ttgagataat caatcactct atatttgtcc gcagtcaatg tgaggatcgg
     9241 tctgcggtca tatttcatct tccaccggag cttctcggta aacgtacgcg gtttctctgc
     9301 cagcttttcg ttatataagt gtttaactat gtccctgctt gttttcatct ctgcgcaaaa
     9361 tggacaaatt gttaatgatt tatatgtatt acgcgggtgt aaacaacgtt ttcgattatt
     9421 ttagtggaat tctctatttc tagattactc atattatata atacacctca tcctttctga
     9481 tatatagtag attgttatgg catatgaaaa agcccgtgag acgaaggcat gaaggtaaat
     9541 aattccgctg atacaacact gccagtcata cagagtatgt ggataggcag tgaattatca
     9601 gtaatggaac agctcagcat catatcattt ttaagcaacg gtcactccta tcatctctat
     9661 acctataatg aagtaaagaa tgtgcccgaa ggcgtcacgc taaaagacgc gaatggaata
     9721 atcaccccgg aca
//
LOCUS       DASSJH010000352         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1161725, whole genome shotgun sequence.
ACCESSION   DASSJH010000352 DASSJH010000000
VERSION     DASSJH010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161725"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFJ67_07780"
     CDS             <1..858
                     /locus_tag="VFJ67_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET7290319.1"
                     /translation="PKIGKGIIPATISENIIDGILRRDIGYDGLVITDSFHMSGISKI
                     GDEWDNSHLALDAGCDIILDPREPVLLIDRLTDMAATGELNPHTLERAAGRVVTAKNR
                     LHTASAGGAQAPARNASALIESIAEGSICRLRGGNLRSRSALVAVFDVTRSDKDVSVS
                     FTETLSGAGIDCRPVHVTFDTRVDKLIAQSKDCEAVICLVYTAVGAWKKQSALPGLYR
                     GVLNELVALPQEKALLSFGSPYVVRGFDKFGTLICAFDLITPCQTAAARVLLGEIDAK
                     GKLPVGTGF"
     gene            894..>2404
                     /locus_tag="VFJ67_07785"
     CDS             894..>2404
                     /locus_tag="VFJ67_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET7290320.1"
                     /translation="MASMGAALTSALSSLSPIDWCIVAAYLLLSLALGVYFTKRASGS
                     MASYFVSDRNMSWWLLGTSMVATTFAADTPLAVTGWVRTEGIWKNWFWWNYVFSHVFI
                     VLVFARLWRRAEVITDNELIEIRYSGRPAAFLRGFKACYFATLFNFIVMGWVISAMAK
                     VLKVFFGVETTAAIVICMFIAFFYTMMSGIWGVALTDFLQYFIALFGTIILAWVVIGS
                     PEIGGFSGFIEKLGTIDASHLSLFMTPSGGTPVSEGFWSSSFFTFLVYVTVIWWSSHN
                     ADGGGYFIQRMCSAKNERHSVAGTAWFAVNHYIIRFWPWVLVALASLIIFSDAGAANG
                     DNEAMYIVVIRDFLGPGLKGLLFVSFLAAFMSTLSTQLNWGASYLMNDMYRRFIKKDA
                     GEKHYIFVSRLCTLVLTLLAGYFALHINNIGKAWIFLWAMSAGIGLVLVLRWFWWRIN
                     AWSEISALASSLLAILIIVIYTRSNGIPLELRHQVLVIPVSVITWIAVTFITNP"
BASE COUNT          487 a          673 c          679 g          565 t
ORIGIN      
        1 cccaaaatcg gtaagggaat tatccccgcg acgatctcgg aaaatataat agacggaatc
       61 ctgaggagag atatcggtta cgacggactg gtcataacgg attccttcca catgtcggga
      121 ataagtaaga taggggatga atgggataac tcccatctcg ctctggatgc cggatgcgac
      181 attattctcg atcccaggga gccagtactg ctcatcgaca ggctcaccga tatggccgcc
      241 accggggagc ttaacccgca cactctcgag agagccgcgg gccgcgtcgt tacggcgaag
      301 aacaggctgc acaccgcatc cgccggaggc gctcaggccc cggcccggaa cgcctccgcc
      361 ctcatcgaga gcatagccga aggctcgata tgcaggctca ggggaggaaa cctgcgttcc
      421 cggagtgccc tcgttgcggt gttcgacgta acacggtcag ataaggacgt ttcagtgagc
      481 ttcaccgaaa cactctccgg ggccgggatc gactgcaggc cggttcacgt cacctttgac
      541 acgcgtgtgg acaaattaat tgcgcaatct aaagattgcg aggccgttat atgccttgtt
      601 tacaccgccg tcggggcgtg gaaaaagcag tccgctctgc ccgggctcta caggggcgtg
      661 ttgaacgagc tggtcgcgct ccctcaggag aaagcgctcc tctccttcgg gtccccgtat
      721 gtagtaaggg gcttcgataa gttcggtact ttgatctgcg ccttcgatct aatcacgcca
      781 tgccagaccg cggcggcccg tgtgcttctc ggagagatcg acgctaaggg taagctcccc
      841 gtcggaaccg gtttctaggg tcactattac ccgaacatct aatcaggtag attatggcct
      901 ccatgggagc agccctcact tccgccctct cctccttaag cccgatagac tggtgtatcg
      961 ttgcggccta cctcttactg tccctggctc tcggtgtgta tttcacgaag agggcgagcg
     1021 gaagcatggc ctcctacttc gtttccgaca ggaacatgtc ctggtggctg ctcggaacgt
     1081 ccatggtcgc aaccactttc gccgccgata ccccactcgc cgtgaccggc tgggtcagga
     1141 cggaaggtat atggaagaac tggttctggt ggaactacgt attcagccac gtcttcatag
     1201 tcctcgtatt cgcgaggctc tggagaaggg ccgaggtcat aacggacaac gagctcatag
     1261 agatcaggta cagcggcagg ccggctgcgt tcctgagggg gttcaaggcc tgttatttcg
     1321 cgaccctctt caacttcatc gtgatgggct gggtcataag cgcgatggcg aaggtgctca
     1381 aggtgttctt cggcgttgag acaactgcgg cgatcgtcat ttgcatgttc atagcatttt
     1441 tctacaccat gatgtccggc atatggggag tggctctaac ggatttcctc cagtatttca
     1501 tagccctctt cggcactatc atactcgcct gggtggtcat cgggtcgccc gagataggcg
     1561 ggttttcagg gttcatagag aagcttggca ccatagacgc aagccacttg tcgctcttca
     1621 tgaccccttc cggcggaacc ccggtgagcg aggggttctg gtcgtcgagc ttctttacct
     1681 ttctcgttta cgtcaccgtc atctggtggt cctcacacaa cgccgacggc ggcgggtatt
     1741 tcattcagag gatgtgctct gccaagaacg agcggcattc ggtcgcgggg acggcctggt
     1801 tcgccgtcaa ccattacata atccgcttct ggccgtgggt tctcgtcgct ctcgcttccc
     1861 ttataatctt ctcggacgcc ggtgcggcca acggggacaa cgaggccatg tatatagtgg
     1921 ttattaggga cttcctcggc cccggcctca agggtctcct tttcgtgagc ttcctcgcgg
     1981 cattcatgtc cacactctcg actcagctca actggggcgc ttcttatttg atgaacgaca
     2041 tgtacaggag attcataaaa aaggacgcag gcgagaagca ttacatattc gtttcccggc
     2101 tctgcacgct cgtgctgacg ctgctggccg gttacttcgc gctccacata aacaacatag
     2161 gaaaggcgtg gatattcctc tgggctatga gcgcgggcat agggctcgtg ctcgtgctga
     2221 ggtggttctg gtggaggata aacgcgtggt cggagatatc ggcgctcgca tcttccctcc
     2281 tcgcgatact tattatagtg atttacacgc gctcgaatgg cataccgctt gagctcaggc
     2341 atcaggtgtt agttatcccg gtatcggtca ttacatggat agcggtcacg ttcatcacga
     2401 atcc
//
LOCUS       DASSJH010000353         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1053247, whole genome shotgun sequence.
ACCESSION   DASSJH010000353 DASSJH010000000
VERSION     DASSJH010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053247"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFJ67_07790"
     CDS             <1..287
                     /locus_tag="VFJ67_07790"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290321.1"
                     /translation="RIPTRRLNDFLKRVIDKTPPPVYKGKDIKFYYISQPLTMQPTFV
                     IFTNEIKGVPENYKRFLESRLREEFPLEGTPVKILFRSERKKDRIKRSGA"
     gene            complement(297..1550)
                     /locus_tag="VFJ67_07795"
     CDS             complement(297..1550)
                     /locus_tag="VFJ67_07795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7290322.1"
                     /translation="MDSLPQVLFINHSVRDGGPGRSLYYLLKFLDRTRLVPYVLIPKD
                     DVFSEWLKAAGIYENVIVEPRFPENLKRPRFGPPGDRGNSGGLSSYLDKLLNFLSVSL
                     NVLDIASLVITSPGLQRKHGITVIYCNGTQAKIAGALMGLVSGRPVIWHVRNIQQTRL
                     LGSLINNLARLRAVKKIICVSGPTASQFRHAKEKLTVIHNGIDIGDYDPQTTTGEIRS
                     AYGLPEGTIIVGSTGRIVPRKGYELFIEAAGIALGELGEKGRGVRFAVVGDTPYFFRD
                     DHLSKLKGLVAGKGLEDSFIFTGFRDDVRPCLRDFDVFVIPSSYPDPFPRSVIEAMSF
                     ALPVVGFKSGGIVESVEDGVTGILSEPGDARQMASAISKLVTDEALRESMGKAGRERV
                     REKFSAQAVAADIQKVILDVSGGGE"
     gene            1695..>2171
                     /locus_tag="VFJ67_07800"
     CDS             1695..>2171
                     /locus_tag="VFJ67_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290323.1"
                     /translation="MNCNLRMILPRGIVSLLFLWFFLIGGCDVEFGSGGDNNGGGGGG
                     GGDEVETVQGTIVSIIPDQDLEGITVEITVDDGAPETDVTDAGGFFSIEGPIAGSPQV
                     AFFDSDSNPLGTIIISVYPTATVELGNISLESGNVNLLNDTEVTFNGDITSNNCTGN"
BASE COUNT          519 a          583 c          582 g          487 t
ORIGIN      
        1 gcaggatacc taccagaaga cttaacgatt ttcttaagag agtgatagac aagacccccc
       61 ctcccgttta caagggaaag gacatcaagt tctactacat atcccagcct ctcacgatgc
      121 agccgacgtt cgtcatattc acgaacgaga taaagggcgt gcccgagaac tacaagaggt
      181 ttctcgaaag caggctcagg gaggaatttc ctctcgaagg gactcctgtc aagatacttt
      241 tcaggtccga gagaaaaaag gacaggataa agagatctgg agcttgatac cccgggctat
      301 tccccaccgc cggatacgtc gagtataacc ttttgtatgt cggcggcgac agcctgagcc
      361 gagaactttt ccctgacacg ctcacggccg gccttcccca tggattcccg taatgcttca
      421 tccgtgacga gcttcgatat ggcgcttgcc atctgcctgg cgtccccggg ctcgctgagt
      481 atccccgtga ccccgtcctc aacggactcg acaatgcccc cggacttgaa accgactacg
      541 ggcagcgcga aggacatcgc ctctatgacc gacctgggga aagggtcggg atagctcgac
      601 gggatcacga aaacgtcgaa gtccctgagg cacggcctca cgtcgtccct gaacccggtg
      661 aatatgaagc tgtcttcgag ccccttcccg gcgaccagcc ctttcagttt gctcaggtgg
      721 tcgtcccgga aaaaataggg cgtatcgccg acgaccgcga atcttacgcc cctgcccttc
      781 tccccgagct ctcctaaagc gatgccggcc gcttctatga aaagctcata ccccttcctc
      841 gggactatcc ttcccgtgct ccccacgata atggtgcctt cagggagtcc gtaggcggac
      901 ctgatttcac ccgtcgtcgt ttgagggtcg tagtccccga tgtcgatacc gttgtgtatg
      961 actgtgagct tttccttcgc atgccggaac tgagacgcag tagggccgga tacgcagatt
     1021 atctttttca ccgcgcgcag cctcgcgagg ttattgataa gggagccgag gaggcgcgtc
     1081 tgctggatgt tcctgacgtg ccaaattacg ggccggccgc tcacgagccc cattagagcg
     1141 cccgctatct tcgcctgcgt gccgttacag tagataactg taatgccgtg ctttctttgg
     1201 agacccggtg aggttataac cagcgaagca atgtcgagca cgttgagcga cacggagagg
     1261 aaattaagca atttatccag gtatgaggac aatcccccgg aattgcccct gtcacccggg
     1321 ggtccgaaac ggggcctttt gaggttttca gggaatctcg gctccacaat gacgttctca
     1381 taaataccgg cagcctttag ccattccgaa aagacgtcgt ccttcggaat caggacataa
     1441 ggaacgagcc tcgtcctgtc caggaattta agcaggtagt agaggctcct ccccggcccc
     1501 ccgtccctga cggagtggtt tatgaaaagc acttggggca acgaatccat atatattcct
     1561 tttttcgggt tatgcaatcg taccatgata aatattaatc ggctaatcgc cgctgattaa
     1621 tgaaaatacg atagtcgcgg gtataatatt aaattaaggc accgataagt tctcgggagg
     1681 atacaggagg attaatgaac tgcaatctca ggatgatttt accgcggggt atcgtatccc
     1741 ttctttttct ctggttcttt ttaatcgggg gctgcgacgt cgagttcgga agcggcggcg
     1801 acaataacgg cgggggcgga ggcggaggcg gcgatgaagt ggagaccgta cagggaacga
     1861 tagtgagcat aattcccgat caggacctcg aagggatcac ggttgaaatt acagtagacg
     1921 acggcgcgcc ggaaactgat gtaacggacg cgggagggtt tttctccata gaaggcccta
     1981 ttgcaggaag cccgcaggtc gcgtttttcg attcggattc gaatcccctg ggtacaataa
     2041 taataagcgt atatccgacg gctacggtcg agctcggcaa tataagtctc gagagcggaa
     2101 atgtcaattt acttaacgat accgaggtca catttaacgg agacattacg tccaacaact
     2161 gtacggggaa c
//
LOCUS       DASSJH010000354         5150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_518517, whole genome shotgun sequence.
ACCESSION   DASSJH010000354 DASSJH010000000
VERSION     DASSJH010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518517"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /gene="lysS"
                     /locus_tag="VFJ67_07805"
     CDS             <1..1239
                     /gene="lysS"
                     /locus_tag="VFJ67_07805"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7290324.1"
                     /translation="IADRSGKIQGYIKKDIIGDDGLKFFKKYVDLGDFIGISGNLFKT
                     RTGELTVNVKDFKLLTKTLHPLPEKWHGLKDVEARYRQRYLDLISNSEIKEIFLTRSR
                     IIKLIRKFLDERDFIEVETPILHPIAGGAAARPFETHHNALGMDLFLRIAPELYLKRL
                     VIGGLERVYEIGRTFRNEGISTQHNPEFTMIEFYQAYATYEDLMDLIEELICYLALEV
                     KGSLVFEYGGAEIDMTRPWKRINVIEHLEKTLGGEFLADDSKLYEKAHSLGVDHKRIR
                     GKAIVEIFEKLAAEELMNPAFVYGFPLDVSPLARKNEADPDITDRFELYITGREIANA
                     FSELNDPIDQKERFRSQLDLKLKGEEEVHEMDEDFVTALEHGMPPTAGAGIGIDRLVM
                     LLTNSPSIREVIFFPHLRPE"
     gene            1236..2546
                     /locus_tag="VFJ67_07810"
     CDS             1236..2546
                     /locus_tag="VFJ67_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7290325.1"
                     /translation="MKYEFFIGLRYLSSRRKQKFASIIGLISVLGVIIGVMALNVVLS
                     VMGGFEEELREKILGVSSHVVILSYDGPMKDYSKVEDEAAKFPGVVGASPFIYGQGMM
                     ASEHNVAGSVVRGIDPGTAGSVTNIEQALGRGMLGGKEEEKDRVPDEQLSRMGKAVLG
                     KLEKETASGKPPILLGRELANTLGVVEGDQVSLVSPFGKMGPFGATAKVKKFEVAGIF
                     DYGMIEYDSSISYVDLKDAMDFFDMSGEASGVEVKVKNIYDARGIGTELASILGFPYY
                     TRNWEEVNKSLFKALRLERIAIAIFLGLIVLVAALDIVSALTMVVMEKGRDIAILRAM
                     GATRNGILKIFIIDGMIIGIVGTLLGSLSGYGICYMLKTSEMIRKLIPFDNNVYPISE
                     FPVKIEPFYFLTVAFFSILICFIATLYPSIQASRKDPVEALRYE"
     gene            2550..3224
                     /locus_tag="VFJ67_07815"
     CDS             2550..3224
                     /locus_tag="VFJ67_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7290326.1"
                     /translation="MEIRVRDLWKVFKTDGGRVEALKGIDLDISDRETLAIVGVSGSG
                     KSTLLHILGTLDQPSSGEVFYGDLNIFKQSDGELAAFRNREIGFVFQFHYLLPEFTAL
                     ENVMMPCLINGLSSENAREMSFDILKRVGLEQRIEHRPGELSGGEQQRVAIARAVVLK
                     PKVILADEPTGNLDLDTGASILDLFLMLNSEYGITSVLVTHNQEITKRLHRRIRLSDG
                     KIVDVN"
     gene            3214..>5150
                     /gene="bamA"
                     /locus_tag="VFJ67_07820"
     CDS             3214..>5150
                     /gene="bamA"
                     /locus_tag="VFJ67_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET7290327.1"
                     /translation="MSINFKAALGVVAVVLLLILNPICGHTAYGQDTAGKGTAPAGTD
                     KGSSENAAPGGGSAEPQAAPEPQKIIQIKIEGNLRVETELIGLNISSKVGEPFSTVTV
                     RDDIKKLYKLGFFEDVTADTEQTPEGVILIYKVKEKPVVSDLRIRGNKDIKSDKILEV
                     IDVKEGRIIDLSKVKQSQEAISALYAQSGLVGTVVDYSIEPEGEGTVSVTYEIKEGKK
                     AYIKKVIIVGNEKLKTKIVKKGLYSKPKGMFSFISKKGLYNQQEIDNDSERIRVTYID
                     NGYLDVKVSKPEITYSEEEKGYIITFKIDEGDQYKVTEITFVGDMIVPEEELRAILEL
                     KTGEIFRGSLLAKDIAGLTTFYGDKGYAFANVEPAFKLDRSNLTVAIQFRIEKGPEVY
                     VRQIDIVGNTRTRDKVIRREIPIEEDQLFNATEVQAIKSRVTRLGFFEDNVEVSTDRV
                     PGTDNQIDIKVKVEEKPTGFFSVAGGFSSVETFIFSGQIQEANLFGYGKRLTLNAQIG
                     GVTQLFYVNYQDPHFLDTNYTLDALLFRTDRQFRDFDRSSWGGSLTIGRYIWRNLRAN
                     VTYRLESVKINGIDENAKLIITREKQTISSIGFGLVWDSRNNLLDPSAGNLSETEVEY
                     AGLGGNTDFIKYTLSSRQWFP"
BASE COUNT         1362 a         1239 c         1493 g         1056 t
ORIGIN      
        1 atagctgaca ggtcggggaa gatacagggc tatatcaaga aggacatcat tggggacgac
       61 gggctgaaat tcttcaaaaa gtatgtggac ctcggcgact tcatcggcat aagcgggaac
      121 ctcttcaaga cccgcacggg cgagctcact gttaacgtta aggacttcaa gctcctcacg
      181 aagactctcc atccgctccc cgagaagtgg cacgggttga aagacgtcga agccagatac
      241 cgtcagcgtt acctcgacct catttccaac tccgagatta aggaaatctt tctcacgaga
      301 agcaggatca taaagcttat acggaagttc ctcgacgaga gggactttat cgaggtcgaa
      361 accccgatcc ttcaccccat cgccggcggg gccgccgcgc gcccgttcga gacacaccac
      421 aacgcgctcg ggatggacct tttccttagg atcgcgcccg agctctatct gaagaggctc
      481 gtgataggcg gcctcgaaag ggtatacgag atcggaagga cgttcaggaa cgaagggata
      541 tccacacagc acaaccccga attcaccatg atagaattct atcaggcgta cgctacgtac
      601 gaagacctga tggaccttat agaggagctt atatgctacc tggctctgga ggtcaagggc
      661 tctctcgtgt tcgagtacgg cggggccgag atagacatga cccgtccctg gaagaggata
      721 aacgtcatag agcaccttga aaaaacactt ggcggggaat tcctcgcgga cgactcaaag
      781 ctttacgaga aagcccattc cctgggcgtc gatcacaagc gcataagagg caaggccata
      841 gtcgagatat tcgagaagct cgcggctgag gagctcatga accccgcctt cgtttacggg
      901 tttcccctcg atgtgtctcc ccttgccagg aagaacgaag ccgaccccga tattacggac
      961 aggttcgagc tctacatcac cggccgcgag atagctaacg ctttctccga gcttaacgac
     1021 cccatagacc agaaggaaag gttcaggagc cagctcgacc tcaagctcaa gggcgaggaa
     1081 gaggtgcatg aaatggacga agacttcgtc acggctctcg agcacggcat gccgcctacc
     1141 gcgggagcgg gcataggcat agaccgcctc gtaatgctgc ttacaaactc cccatccata
     1201 agggaagtca ttttcttccc ccatctaagg ccggaatgaa atacgaattc tttatcgggc
     1261 tcagatacct aagttccagg cgcaagcaga agttcgcctc cataataggg cttatctcgg
     1321 tcctgggcgt aattataggg gtaatggccc tgaacgtggt cctttcggtc atgggcgggt
     1381 tcgaggagga gctgagggag aagatactgg gtgtgagctc ccatgtggtg atactcagct
     1441 acgacggtcc tatgaaggat tactccaagg tagaggacga agccgctaaa ttccccggcg
     1501 tggtcggggc tagccccttt atttacggtc aggggatgat ggcgagcgag cataacgtcg
     1561 cgggctcggt cgtacgcgga atagacccgg ggacggcggg ttcggtgacc aacatcgaac
     1621 aggcgctcgg ccgcgggatg ctgggcggca aggaagagga gaaggacagg gtgcccgacg
     1681 agcagctgtc gaggatggga aaagctgtgc tggggaagct cgaaaaagaa actgcgtcgg
     1741 ggaaaccccc tatactgctc ggcagggagc ttgccaacac gctcggggta gtggaggggg
     1801 accaggtgag cctcgtatcg ccattcggca agatgggtcc tttcggcgcg accgccaagg
     1861 tgaagaagtt cgaggtggcc ggaatattcg attacggcat gatcgagtac gattcctcca
     1921 tctcttacgt cgatctcaag gacgcgatgg acttcttcga catgagcggg gaggcttccg
     1981 gggtggaggt caaggtcaag aatatatacg acgcgagggg cattggcacc gagctcgcct
     2041 ctatactggg gttcccctat tatacgagga actgggaaga ggtgaacaag agcctcttca
     2101 aagcgctcag gctcgagagg atagcgatag ccatatttct cggtctgatc gtactcgtag
     2161 ccgcgctcga tatcgtgagc gccctaacta tggtggtaat ggagaagggg agagacatcg
     2221 cgattctccg ggcgatgggg gccacgagga acgggatact taagattttc atcatagacg
     2281 gcatgatcat aggcatcgtg gggacccttc tcggaagcct ctccggctac ggcatctgtt
     2341 atatgctcaa gacgagcgaa atgataagga aactgatacc ttttgataat aatgtctatc
     2401 ctatttcgga gtttccagtt aaaattgaac cgttttattt tcttactgta gcatttttca
     2461 gcatattgat atgttttatc gcaacattgt atccgtcaat ccaggcgtct cgaaaagatc
     2521 ctgttgaggc actgaggtat gagtgaggaa tggagattcg agtacgagat ttatggaagg
     2581 tatttaaaac ggatggcggt cgggtggagg ctctcaaggg aattgatctt gacatatcgg
     2641 accgggaaac gctcgcgata gtcggtgtct caggatcggg gaaaagcact cttcttcata
     2701 tcttaggaac gctcgaccaa ccgtcctcgg gtgaggtgtt ttacggtgat cttaatattt
     2761 tcaagcagag tgacggcgag cttgcggcct tcaggaacag ggagatagga tttgtcttcc
     2821 agttccatta tctgctgccc gagttcactg cattggagaa tgtcatgatg ccgtgtctta
     2881 taaacggact gagctcggaa aatgcgaggg aaatgtcgtt cgatatattg aaaagggtag
     2941 gtttggagca gagaatcgaa caccgtccgg gtgagctatc gggcggggaa cagcagaggg
     3001 tcgcgatagc gcgggcggta gtactcaagc cgaaggtaat actggcggac gagccgacgg
     3061 gcaacctgga cctggataca ggcgcgtcta ttctagacct gttcctgatg ctcaactccg
     3121 aatacgggat cacatcggta ttggttacgc ataaccagga aatcactaag cggctccaca
     3181 ggagaatcag gctttccgat gggaaaatcg tcgatgtcaa ttaattttaa ggctgcgctc
     3241 ggcgtggttg cggtcgtact gctgcttata ctgaacccga tatgcgggca taccgcctac
     3301 gggcaggaca cggccggcaa ggggactgcc ccggcgggta cggacaaggg ctcctctgaa
     3361 aacgccgccc cgggcggtgg gagtgccgag cctcaggcgg ctcccgagcc ccagaaaata
     3421 atccagataa aaatagaggg gaatctccgt gtcgagaccg agctcatagg cctgaacatc
     3481 tcttcaaagg tcggggagcc gttttccacc gttacagtga gggacgacat aaagaagctc
     3541 tataagctcg gattcttcga ggacgtcacg gccgatacag agcagacccc ggaaggcgtc
     3601 attcttatat ataaggtcaa ggaaaagccc gtcgtctccg acctcaggat caggggtaac
     3661 aaggacataa agagcgacaa aatcctcgag gtcatagacg tcaaggaagg ccgtattata
     3721 gacctcagca aggtcaagca gagccaggaa gcgataagcg ccctatacgc tcagagcgga
     3781 ctcgtcggca ctgtggtgga ttacagcatt gagcccgaag gcgaggggac ggttagcgtc
     3841 acctacgaga taaaagaagg taaaaaggca tacatcaaga aggtgataat tgtcgggaac
     3901 gaaaagctca agaccaagat cgtcaagaaa gggttatatt ccaagcccaa gggcatgttc
     3961 tcgttcattt ccaagaaggg cctctataac cagcaggaaa tagataacga ttccgagagg
     4021 ataagggtaa cttacatcga caacggctat ctcgatgtaa aggtgagcaa gcccgagata
     4081 acgtacagcg aagaggaaaa gggttatatc attacgttca agatcgacga gggcgatcaa
     4141 tataaagtaa ccgaaatcac gttcgtcggg gacatgatcg tccccgagga agagctccgg
     4201 gcgatactcg agctgaagac cggggaaatt ttcagaggca gcctgctcgc caaggacatt
     4261 gcgggactca cgacctttta cggcgacaag ggatatgcat tcgccaacgt ggagccggcg
     4321 ttcaagctcg acaggtcgaa cctgactgtg gccatacagt tcaggatcga aaagggcccc
     4381 gaggtatatg tgaggcagat agatatcgtc ggcaacacga gaaccaggga caaggtcatc
     4441 aggcgggaaa tacccataga ggaagatcag ctgtttaacg ccacagaggt ccaggcgatt
     4501 aaatcgaggg tcacgaggct cggattcttc gaggacaacg tggaggtatc caccgacagg
     4561 gttccgggta cggataacca gatcgacata aaggtaaagg tcgaggagaa gccgaccggg
     4621 ttcttcagcg tcgccggcgg attcagctct gtagaaacgt ttattttttc cggccagata
     4681 caggaagcga accttttcgg ctatgggaag aggcttacgc tgaacgcgca aatcggcggc
     4741 gtcactcagc ttttctatgt aaattatcag gacccccatt tcctcgatac caactacacg
     4801 ctcgacgcgc tcctgttcag gacggacagg cagttcaggg atttcgacag gagttcgtgg
     4861 ggaggcagtc tcacaatagg cagatatata tggagaaacc tgagggccaa cgtaacttac
     4921 aggctggaaa gcgtaaaaat aaacgggatc gacgagaacg cgaaactcat tataacgaga
     4981 gaaaagcaga ctatcagcag catagggttc ggtcttgtct gggattcgag aaataacctt
     5041 ctcgacccga gcgccgggaa cctctccgaa accgaggtcg aatacgctgg cctgggtgga
     5101 aatactgatt ttattaaata tactctatcg tcgaggcagt ggttcccctt
//
LOCUS       DASSJH010000355         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1492474, whole genome shotgun sequence.
ACCESSION   DASSJH010000355 DASSJH010000000
VERSION     DASSJH010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492474"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFJ67_07825"
     CDS             <1..255
                     /locus_tag="VFJ67_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290328.1"
                     /translation="AVAYIITLMVLVLLVSSYMSNGLRTYRRVLLILICLIAVQISLG
                     VGVVQTKLNFAVTALHLSFALLILSLVLYTWFEGMEESYK"
     gene            283..1197
                     /gene="cyoE"
                     /locus_tag="VFJ67_07830"
     CDS             283..1197
                     /gene="cyoE"
                     /locus_tag="VFJ67_07830"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET7290329.1"
                     /translation="MNTIGQVDRAPAKIVVSTILLSKPGIILSVAFTGFAGMVLANRG
                     IPSLVLTVLAVLSLLLSAAGSAILNNVLDKQIDTLMSRLSKRVDALEVVGERTATIIS
                     LIFIFISLFISFYYLNAVNGALIVAAILSYTLLYTLYLKRSSPYGTILGGLPGALPVL
                     VGYTAVNPHIGVDGIILFAFMMLWQPPHFWALAQKYKLDYKKAGVPVMPVALGSKFTN
                     IMILLYSIALLPLSLSLWALHYCSDYYAVFAIISGLYFEYVIIRSAVKNTDYGKAFGA
                     SIVYMLVIMLSLILDLSLGSPNAVVGSF"
     gene            complement(1247..>2808)
                     /locus_tag="VFJ67_07835"
     CDS             complement(1247..>2808)
                     /locus_tag="VFJ67_07835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET7290330.1"
                     /translation="LNEDYLREEAEMSALGAEFSKLKVELMRGLKDASDEDFASLAES
                     EGLSSSAYYLGRLREESQRRMGRENEILAADLGVDGISAWGRLYDTVSGKLEFEMTYP
                     DGTVKILPISQRRSLMEKPDRAVRKAAFEGGNRAWQSIEDTAAAALNAIAGTRLTLNR
                     HRGIDHFLDVALFQSSISRKTLDAMFEAIYSELEIPRSILRLKARTMNRDSVAWYDLG
                     APLEFDSGQKLTWTRAQKMVKNSFAASYPRLGKFMDGVYKKEWIDWEPREGKRPGGFC
                     TGSLLTKESRIFMTYNETLGDVLTLAHEAGHAFHSHVMSDIRPYSHFYPMTLAESAST
                     FGEMILTEGVLEDPSINDTEKALTLDMEINHGAIYLMDIPVRYEFEKAFYEERQKGEV
                     SVSRLKELMADTQRRIFGDVLEEGGEDPYFWASKLHFYITGITFYNFPYTFGYLLSRG
                     LFSMFKREGKEFLPKYEEFLRLSGSDTAESVAKMSIERDLESPGFWVESINSLKEPLS
                     RLKELLRDMKS"
BASE COUNT          563 a          808 c          711 g          726 t
ORIGIN      
        1 gctgtcgcat acattattac acttatggtg ctcgtgcttc tcgtctcctc gtacatgagt
       61 aacggcctcc ggacgtaccg gagggtactg ctgatcctca tatgcctcat cgccgtccag
      121 atatccctcg gggtgggtgt ggtccagact aagctgaact tcgcggtcac ggcacttcac
      181 ctttcctttg cactgttaat actctcgctc gtcctttata cttggttcga gggcatggag
      241 gaaagctata aataatccgc acatgaggaa aagaagggtt agatgaatac gatagggcag
      301 gtcgacaggg ctccggcaaa gatagtggta tctacaatac tgctctccaa gcccgggata
      361 atactgagcg tagccttcac gggtttcgcg ggaatggttc tcgccaaccg cgggatccct
      421 tctctcgtat taactgtgct ggcagtgctt tcgctgctcc tgagcgccgc gggctcggcc
      481 atactcaaca acgtcctcga taagcagatc gatacgctca tgagcaggct cagcaagcga
      541 gtggatgcgc tcgaagtggt cggcgaaagg acagcgacaa taatatcact tatatttatt
      601 tttatttcgc tgtttatttc cttttattac ctgaacgcgg tgaacggcgc gctgatagta
      661 gccgcgattc tgagctacac gctcctctac acgctttatc tcaaacgcag ctccccttac
      721 ggcactatac tcggtggtct cccgggagcg ctgccggtac tggtcggata taccgcggtc
      781 aacccccata tcggagtgga cggaataata ctgttcgcat tcatgatgct ctggcagccc
      841 cctcatttct gggccctcgc acagaaatac aagttagatt acaaaaaagc cggagtgcca
      901 gttatgcccg tcgctctcgg gtcgaaattc acaaacatca tgatactcct ttattcaata
      961 gccctgctgc cgctctccct ctccctctgg gcgcttcatt actgctcgga ttactatgcg
     1021 gtcttcgcca ttatttcggg gctctatttc gagtacgtga ttataaggag tgccgtaaaa
     1081 aataccgatt acggaaaggc tttcggcgcc tcgatcgtgt atatgctcgt cataatgctc
     1141 tcacttatcc tagacctttc tttgggctct ccgaacgccg tagtcggaag tttttagggt
     1201 ccggttcggt aatagttctt caagtgtctt caactcgtca tgcgggtcat gatttcatat
     1261 cgcggaggag ctccttcagc cgcgataaag gctctttgag cgagtttatg gactcaaccc
     1321 agaatcctgg ggactcgagg tccctctcta tgctcatctt cgccacgctc tccgccgtgt
     1381 ccgagcccga gagcctcagg aactcctcgt atttgggaag gaactctttc ccctcccgct
     1441 tgaacataga gaacaggccc ctgctcaaca agtaaccgaa cgtatacggg aaattgtaga
     1501 acgtaatccc cgttatatag aaatggagct tcgaggccca gaaataaggg tcctcgcctc
     1561 cctcttcgag cacgtcgccg aagatcctcc gctgtgtgtc ggccattagc tccttgagcc
     1621 tgctgacgct tacttcccct ttctgcctct cctcgtaaaa cgccttctcg aattcgtacc
     1681 tgactgggat atccatgagg tatatcgctc cgtggtttat ttccatgtcg agcgtgagcg
     1741 ccttctcggt atcgtttatg gaagggtctt cgagcacgcc ttcagtcagt atcatctctc
     1801 cgaaagtgga cgccgattct gcgagcgtca tcggatagaa atgggagtag ggccggatgt
     1861 cgctcatcac atggctgtgg aacgcgtgtc ccgcctcgtg cgcgagtgtt agcacgtctc
     1921 cgagcgtctc gttgtatgtc atgaatatcc tggactcttt cgtaaggaga gagccggtac
     1981 agaaccctcc gggacgcttc ccttcgcgcg gctcccaatc gatccactcc tttttatata
     2041 ccccgtccat gaacttgccg agcctcggat aagacgccgc gaaggagttc ttaaccatct
     2101 tctgcgcgcg tgtccacgtg agcttctgcc cggaatcgaa ttcgaggggc gctccgaggt
     2161 cgtaccaggc gacggaatcg cggttcatcg tcctggcctt gagccttagt atgcttctcg
     2221 gaatctccag ctcggaatat atcgcctcga acatagcgtc gagggtcttt ctgcttatgg
     2281 aagactggaa tagagcgacg tcgaggaaat ggtctatgcc cctgtgcctg ttaagcgtga
     2341 gccgcgtgcc cgcgatcgcg ttaagggccg cggcggcggt gtcctctatg ctctgccagg
     2401 ccctgttgcc tccttcgaaa gcggcctttc tgactgccct gtccggcttt tccataaggg
     2461 atcgtctctg agagatcggt agaattttaa cggttccgtc ggggtatgtc atctcgaact
     2521 ctagcttgcc cgataccgtg tcgtagaggc gcccccaggc gctgataccg tccacgccca
     2581 ggtcggcggc gagtatttcg ttctcgcggc ccattcgcct ctgagactct tcccgcagcc
     2641 taccgagata atacgcggag ctcgataacc cttcgctttc cgcaagcgac gcgaagtctt
     2701 cgtccgaggc gtccttcagc cctctcataa gctccacttt gagcttcgag aattccgcgc
     2761 cgagagccga catctcggcc tcttccctga ggtagtcttc gttgagcc
//
LOCUS       DASSJH010000356         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_302309, whole genome shotgun sequence.
ACCESSION   DASSJH010000356 DASSJH010000000
VERSION     DASSJH010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302309"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFJ67_07840"
     CDS             complement(<1..597)
                     /locus_tag="VFJ67_07840"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7290331.1"
                     /translation="MRERNIKSFIGLHALILVALFTGCQQVKEIPDEIPEQLPKPVRD
                     FTERDNYIIGLNWYKQWNYGIAAKYWKPLATEGDCDALYALGLLYFEGLGVGQSYDKA
                     IELWTRSADQGQAQAQISLGVVYARLDLPYTSLDCTRGCGTEKELPEAYKWFGTARQI
                     GSPREKRIAEDSLNKIIPEMTPEEVGEGDSLVSGWKPDP"
     gene            complement(733..1911)
                     /locus_tag="VFJ67_07845"
     CDS             complement(733..1911)
                     /locus_tag="VFJ67_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290332.1"
                     /translation="MKVTLVNLACPSYALPNRTSVSSAQLAEADRIKHVCKVVLTWWY
                     ALPSQTWCPYMRQNSSVEYCGPYDNWGFISKVDNHDSFNLGGDIYHDFILPDHPDWIL
                     RDAGGGVVTNNFIPNEAAPDHGNMSFVDFYFNFFIQVPSSIAGARWKGTYKERSWNLR
                     FLDNYIVYLPAAWSSPPVNPDTGAVLSREDRERDILDATKRLRQRADNEAGGLRYIAN
                     IWNDVEASYLDREIYPELMMYVDYALFEAWTTDLQGVPVSEEVWLRRVTAAQDMVENR
                     RAEPVVQVGVGDFWYALSSLLLVSEKGKGMIWSNQMYSDALIQQLSALDLGDPLELFT
                     FVDNAYQREWEKGKVIVNPSDDATVEISLEESYQDAASGEMVTAVTLPPKSGKLLLIP
                     "
BASE COUNT          549 a          615 c          565 g          510 t
ORIGIN      
        1 gggatcgggc ttccagccgc tgacgagcga atccccttcc ccgacctctt caggcgtcat
       61 ttcgggaatt attttattga gtgaatcttc cgcgatccgc ttctcgcggg gggatcctat
      121 ctgccgcgca gtgccaaacc atttataagc ttcgggcagt tccttttccg tcccgcatcc
      181 cctggtgcaa tcgagggaag tatatgggag atcgagtctc gcatagacga cgccgagact
      241 gatctgggcc tgagcctgcc cttggtcagc cgatcgtgtc caaagctcta tcgctttatc
      301 ataactctga ccgacgccca ggccttcgaa atacaaaagc ccgagcgcgt aaagggcatc
      361 acagtcgccc tccgtggcga gaggtttcca gtatttggcg gctatcccgt agttccattg
      421 cttataccag ttaagcccga ttatgtagtt gtccctctcg gtaaagtctc tcacaggttt
      481 cggcagctgc tcgggtatct catcaggtat ttccttcacc tgctgacagc ccgtgaacag
      541 tgcgactaat atgagggcat gcagcccgat aaaagattta atattccttt cccgcaccag
      601 gtactccgtc caaagtccgc cggacagcat aggcagcaaa attcactttc aaatatagtg
      661 gatgggaata tgatgtcaaa catctccggc tctcatccgg aagaaaaggt tgcaaaagtg
      721 tggatacaga tttcagggta tgaggagcag cttgccggat ttcggtggta gggtaacggc
      781 tgtgaccatc tccccgctcg cggcatcctg atagctctcc tcgagagaaa tttcgaccgt
      841 agcatcgtcc gaaggattaa ctatcacttt ccccttctcc cactcccttt gataggcgtt
      901 gtcgacaaag gtaaagagtt ccagagggtc ccccagatcg agggcgctca actgctgaat
      961 cagggcgtcc gagtacatct gattcgacca gatcatgccc ttgcctttct cgctgacgag
     1021 aaggagtgat gaaagggcat accagaaatc gccgacgccc acctgaacca cgggctcggc
     1081 cctcctgttc tcgaccatat cctgggcggc cgtcaccctc cggagccata cttcctccga
     1141 gacaggcacg ccctgaagat ctgtagtcca ggcctcgaac agcgcatagt ccacgtacat
     1201 catgagctcg ggataaattt ccctgtccag atacgaggct tccacgtcgt tccagatatt
     1261 agcgatatat cttagccctc cggcttcgtt atcggccctt tgcctgagcc gttttgtggc
     1321 gtcaaggata tcccgctctc tatcctcgcg ggagagaacg gcgcctgtat ccgggttcac
     1381 gggcggtgag ctccacgccg cgggcagata aacgatgtag ttatccaaaa acctcagatt
     1441 ccacgaccgc tccttatatg ttcctttcca tctcgccccc gcgatcgagg aagggacttg
     1501 gatgaagaag ttaaagtaga aatcgacaaa actcatattc ccgtggtcgg gggcggcctc
     1561 gttgggaata aaattattgg tgacgacccc tccgcccgcg tcccgtaata tccagtcggg
     1621 atggtcaggc agtatgaagt cgtggtagat gtctccaccc aggttaaacg agtcgtggtt
     1681 gtcgactttg cttatgaaac cccagttatc gtagggaccg caatactcca ccgacgagtt
     1741 ctgcctcata taaggacacc aggtttgaga aggaagcgca taccaccagg taagcacgac
     1801 cttgcagacg tgctttatcc tgtccgcttc cgccaattgg gcggaggaca ctgaagtccg
     1861 gttaggaaga gcgtaagagg gacaggccag attgacgagt gtgactttca acggagaacc
     1921 cggagaatcg gaagaagacc cgccgccccc acacgccgcc gcaacgaaca tcaacccggc
     1981 aacggttttg agcaaacaag accgcattaa gaaaaatcac cccattcctc taacacatcg
     2041 agtgcatcag aaggaaacag tagtatagca gagaatatca gacgtaacag catctatttg
     2101 acaaaaaggt cacgaggtga aagaattaac gggttaaatt cgggacaggg tcaataatgc
     2161 gtttaccgga atactttaaa tgtggagaga aacggcctgt aatctctttt gaaatttttc
     2221 cgcccaagac cgaaggagg
//
LOCUS       DASSJH010000357         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1492604, whole genome shotgun sequence.
ACCESSION   DASSJH010000357 DASSJH010000000
VERSION     DASSJH010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492604"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFJ67_07850"
     CDS             <1..394
                     /locus_tag="VFJ67_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290333.1"
                     /translation="EHVIERFEPFPVFGLFGTRLLFVGVFIHSRDPWGLGKIYNKNNN
                     ALSLLKSTVIKHGICARTQCVPKYDPTVTLLLKSATKQIPFDLLVRRSLLSSNAMADE
                     DGEAGSDRSNLVLSLFITSPSWRGEGVP"
     gene            548..1981
                     /gene="lpdA"
                     /locus_tag="VFJ67_07855"
     CDS             548..1981
                     /gene="lpdA"
                     /locus_tag="VFJ67_07855"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7290334.1"
                     /translation="MEKFDLTVIGSGPGGYIAAIRAAQLGMKVAIIERDKAGGVCLNW
                     GCIPSKSILKCAEVYQYFKHSGEFGIEHKGLSYDFSKVIDKSRNASDTLTKGVEFLLK
                     KNKATLFKGVGKMITKNKVGVVQEKGQIEIDTDKILIATGSVPMTLPGLEIDGKQIMT
                     SDEAIFHRAVPDSVVVIGGGYIGAEFAYVYSSFGSKVTIVEMLDHLIPGADEEVSREL
                     EKDFKKNGIDVLTAAKFKEVKKKKKNVDVIVEKLADKSEATITASMVLVAVGRRPIAN
                     DAPTSLSYYKKSGDIGLSDLRIELNERGFIRTDGSYMTTCDNVYAIGDVIGPPLLAHK
                     ASEEGVAAVEMMAGLKSKVHYDNIPGCVYCQPEISMIGMTEKQAKEKGLDYTVGKFPF
                     KASGKAVGTGETDGFVKIIADKSTGEILGGHIIGHGATELIAEIAVGRTLETTPLEIA
                     ITSHAHPTLSEAVMEAALAALGRARNI"
BASE COUNT          558 a          544 c          561 g          418 t
ORIGIN      
        1 tgagcacgta atcgagagat tcgagccatt ccctgttttc ggtctcttcg ggaccaggct
       61 tctctttgtc ggtgttttta tccattcgcg ggacccgtgg ggcttgggga aaatctataa
      121 taaaaataat aatgcactga gtctccttaa gtcaaccgtt attaagcacg gaatctgcgc
      181 tcgcacacaa tgtgtgccta aatacgatcc tactgtaact cttctgctta aatcagcaac
      241 gaaacaaatc ccgttcgacc tgcttgtccg gcgtagcctc ttgtcttcca atgccatggc
      301 ggatgaggat ggcgaagccg gaagcgaccg tagtaatctc gtcctttctc tttttatcac
      361 ctctccctct tggaggggag agggtgtacc ttaaaagaaa taaatcggtc attagcggga
      421 tcgcggctgg aagccgctcc tacagagtct tggatttatt gtcatataga gagtcattcc
      481 ccgtaactca atctcccctc caaaaataat tgacgcccct cccattgaat atataatagt
      541 ccctgccatg gaaaaatttg acctcacagt gatcggatcg ggacccggcg gatacatcgc
      601 cgcaataaga gcggcacagc tgggaatgaa ggttgcaata atagaaaggg acaaagccgg
      661 aggcgtctgt ctcaactggg gctgcatccc ttccaaatcc atactcaaat gcgccgaagt
      721 ctatcaatat tttaaacatt caggcgagtt cggcatagaa cataaagggc tttcttatga
      781 cttctccaag gtcatagaca agagccggaa cgcctccgat acgctcacga aaggcgtgga
      841 gttcttactt aagaagaata aagccacgct cttcaaaggc gtcggcaaaa tgattacgaa
      901 aaacaaggtc ggagtcgtgc aggagaaggg ccagatagag atcgacaccg acaagatatt
      961 aatagccacg ggctcggtac ccatgaccct ccccggtctc gagatagacg gcaagcagat
     1021 catgacgagc gacgaggcca tttttcacag agcggtcccg gactcggtcg tcgtcatagg
     1081 gggcggatac ataggggccg aattcgcata cgtctacagc tcgttcggaa gcaaggtgac
     1141 cattgtcgag atgctagacc accttatccc cggcgcggat gaagaggtgt cgagggagct
     1201 ggagaaggat ttcaaaaaga acggcataga cgtcctcacc gcggcgaaat tcaaggaagt
     1261 gaagaaaaag aaaaagaacg tcgatgtaat cgtcgagaag ctcgccgaca agagcgaggc
     1321 gactattacc gcatcgatgg tcctagtcgc cgtcggcagg cgcccaatcg cgaacgacgc
     1381 gcccacgtcg ctcagctact acaagaaatc gggcgacatc ggacttagcg accttcgcat
     1441 agaattgaac gagaggggat tcatcaggac ggacggctcg tacatgacca cgtgcgacaa
     1501 cgtctacgcc ataggagacg ttatcggccc gccgctcctc gctcacaagg cgtccgagga
     1561 aggcgtcgcg gctgtcgaga tgatggccgg gctcaagagc aaggtacatt acgacaacat
     1621 ccccggctgc gtatactgcc agcccgagat atccatgata ggcatgacag agaagcaggc
     1681 caaagaaaaa gggctcgact acaccgtagg caagttcccg ttcaaggcgt cgggaaaggc
     1741 cgtgggcacg ggcgagacgg atggtttcgt caagataatc gccgacaaat cgacgggaga
     1801 gatattaggc ggccacatca taggccacgg agcgaccgag ctcatagccg agatcgctgt
     1861 gggcaggaca ctcgagacga cgccgcttga aatagcgata acgtcccacg ctcaccccac
     1921 cctgtccgaa gcagtaatgg aagcggccct cgccgccctc ggcagggcta ggaatatata
     1981 atatatatta gcgatactgg acatcaggtc cgttcgtcct gcttgtccgg cgaagcaatg
     2041 gcgctcgtct cggcgaaggc ccgagccgta gccgggaagc t
//
LOCUS       DASSJH010000358         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_944181, whole genome shotgun sequence.
ACCESSION   DASSJH010000358 DASSJH010000000
VERSION     DASSJH010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944181"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFJ67_07860"
     CDS             complement(<1..517)
                     /locus_tag="VFJ67_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290335.1"
                     /translation="MKTVLPVLLTVLLFLSACGQSGTKESSGEKAGTDNQAESSNKTT
                     GRVKPEKSGKVVAKVNGVPIYQDELNGRPVESLITDEILYQEGLSEGLEKKYQEKVMQ
                     YQMSLVVREVKDKIMSGMPPEKEVTEQELLDYYNSAKDINYTNLRVEEINFTDQKLGD
                     KILEMAKAGKDF"
     gene            705..2264
                     /locus_tag="VFJ67_07865"
     CDS             705..2264
                     /locus_tag="VFJ67_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET7290336.1"
                     /translation="MKVLLLEAGPRYNPYTSYALDEDDWETKRFPEIKKIRNTFGKKQ
                     PLDTKYEGLRSWNKASGKLNPSKERRYLKYHQISGIGGTTLHYQGEAHRLNENAFRMK
                     SRYGLAVDWPIGYKDLEPYYSEAEGILGVAGPENIPDRPRSKPYPLKPHRLSYWSKMV
                     EKACGKQGIELIPNSVAILSAPYRDAPPCNYCNGCVWGCPRKDKGSVDVTFIPIAEKT
                     GNCEILDNVYVTRIQVEKKLGGSKAKGVVYYDERGKEHFAGGDYIAVACGAVETPRLL
                     LNSEINNNSLVGKNFMETNFYEVIAMHPERMDAYRGIPMDSIIWKWNNPDPEKGRSGG
                     LRMFPSAGSTLGPVNYSLRYFEGWGESFVKEMEKWFGHAISMGGIAEFLPNKDSFVTL
                     DPKVKDQYGSPVAQIQSFLGEPELKSLEFISKKSKEILKAAGAGDIVEEVSSYDFFSA
                     THVYGTCRMGNDRETSVVNSSLRVHNIPNLLVTDASVFPTSGGGEAPSLTIEALSLRA
                     ADLLLKDIKKG"
BASE COUNT          581 a          640 c          651 g          575 t
ORIGIN      
        1 tgaagtcttt gccggccttg gccatttcta atatcttatc gccgagtttc tggtcggtga
       61 aatttatttc ctcgaccctt aagttggtgt aattaatatc tttggccgaa ttgtaatagt
      121 ccagcagctc ctgctcggtt acttcctttt ccggcggcat gccggacatg atcttgtcct
      181 tgacttctct gacgaccagg ctcatctggt attgcatgac cttctcctgg tatttcttct
      241 caagaccctc gctgaggcct tcctggtaca gtatctcgtc tgtgataagc gattccacgg
      301 gccggccgtt gagctcgtcc tggtatatcg gcacgccgtt aacctttgct accacttttc
      361 cgctcttttc gggcttgacc ctgccggtcg ttttattcga gctctctgcc tgattgtcgg
      421 tgcccgcctt ctctccggag ctttcctttg ttccagattg gccgcaggcc gataggaata
      481 gcagaactgt gagcagaacg ggtaaaacgg tcttcatcaa ttgtctcctt ttcaaagtgg
      541 cgggtaaatc aaatgtataa cttataaatt gacacaaagc aagagtttca ttatattatc
      601 aaccgctcac tgccatgccc actgaaaaag aaaaatacga ttgcgtagtc ctaggatcgg
      661 gccccggcgg agcccctttc gcgtggaaat tggcttcaaa cggcatgaaa gttttgctgc
      721 tcgaggccgg cccccggtac aacccctaca cgagctacgc tctcgatgag gatgactggg
      781 aaaccaagag gtttcctgaa atcaagaaga tacgtaacac cttcggcaaa aaacagcccc
      841 tggacaccaa atatgagggc ctgaggtcat ggaacaaggc ttccggcaag ctgaacccgt
      901 ccaaagaaag gagatatctg aagtaccatc agatttccgg cataggcgga acgacgctcc
      961 actaccaggg cgaagctcac agactcaacg agaacgcttt ccgcatgaag agccgctacg
     1021 ggctcgcggt cgactggccc ataggatata aggacctgga gccctactat tccgaggccg
     1081 agggcatttt gggtgttgcc gggcctgaaa atatacccga ccggcccagg agcaaaccgt
     1141 atcccctcaa gccgcaccgg ctcagctact ggagcaagat ggtggagaag gcttgcggca
     1201 agcagggcat cgagctgata ccgaattcgg ttgcaatact ttccgctcct taccgcgatg
     1261 cgcctccgtg taattactgc aacggctgcg tctggggctg tccccggaag gacaagggca
     1321 gcgttgatgt gactttcata cccatcgccg aaaagaccgg caactgcgaa atactcgata
     1381 acgtctatgt cacacggata caggtcgaga agaagctagg ggggagtaag gccaaagggg
     1441 tagtatatta cgacgagagg gggaaggagc atttcgcggg cggcgattat atcgcggttg
     1501 cctgcggggc tgtggagacg ccgaggcttc ttctgaactc cgaaattaac aataacagtc
     1561 tcgtgggcaa gaatttcatg gagacgaatt tctacgaagt catagccatg catcccgaaa
     1621 ggatggacgc ataccgcggg atacccatgg acagcattat atggaaatgg aataaccccg
     1681 atccggaaaa agggcgttcc ggcggcctca ggatgttccc ctcggcgggc agcaccctgg
     1741 gtccggtgaa ttattccctc cgctatttcg aaggatgggg cgagagcttc gttaaagaga
     1801 tggaaaagtg gttcggacac gcgatctcta tgggaggtat cgcggaattc cttcccaata
     1861 aagacagctt cgtcacgctc gaccccaagg tcaaagacca gtacgggtcc cccgtcgctc
     1921 aaatacaatc ttttctcggg gagccggagt taaagtcact ggaatttatt tccaaaaaat
     1981 cgaaggagat tctaaaagcg gccggtgcgg gtgatatagt cgaggaagtc tcctcctacg
     2041 atttcttttc ggcgacccac gtctacggga cctgccgtat gggcaatgac cgcgagacat
     2101 ccgttgtaaa ttcctctctg agagtccaca atatccccaa tctgctggtg accgacgcta
     2161 gcgttttccc gacatcggga ggaggcgaag ccccatcgct cactatagaa gctctgagtc
     2221 tgcgggcagc cgaccttctg ttgaaagaca tcaagaaagg ttgaataact tttgactcga
     2281 ttcctctcct ggcagccgtt catcgtaatc atcgtctttg gggctttcgt cgcgatcggt
     2341 tccggggcat atgcggagga tgttccgaaa ttcatcgata cccgggatga tgcgggactc
     2401 tatcccgcgg gcaggctcgg tcaattggcg gttgggggag actataa
//
LOCUS       DASSJH010000359         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_519305, whole genome shotgun sequence.
ACCESSION   DASSJH010000359 DASSJH010000000
VERSION     DASSJH010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519305"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFJ67_07870"
     CDS             complement(<1..549)
                     /locus_tag="VFJ67_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290337.1"
                     /translation="MRFCSKVLFISLLFLTASLAEEPAGNYSRQEISPDDLMTHVRYL
                     ASDELCGRMTGEKGAEKAAVYIARNFEEAGLRPQGDGGTYFQKFPLPDKLIPGNDNSL
                     TVTLNGHGKQLGLGSDFFPLSLSASGSVSGEIVFAGYGISAPELGYDDYRDLDVGGKI
                     VLLLRGSPMGMDYRSNFYDYASP"
     gene            688..1449
                     /locus_tag="VFJ67_07875"
     CDS             688..1449
                     /locus_tag="VFJ67_07875"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7290338.1"
                     /translation="MYPVLFRVGDISVSSFSVMVLLGFLVAYILGESEFKRKGINGNL
                     VDLLLIASVIGGLGGAKIMFLYQQVPITSFIADPVRYLASGFTFFGGLIGAAILTWLV
                     TQMKRISFLTVTDLMCPLLIIAYAIGRTGCLLVGDDYGNPSDLPWAMAFPEGSPPTTL
                     RVHPTQIYDTICMAILFAVLWGIRKRNFPTGRMTAITFITLGVQRFLVEFLRHTTPSF
                     IPGISQAQIIAVLFVAAGIVILLKTKRGAQAGAHS"
     gene            1703..2623
                     /locus_tag="VFJ67_07880"
     CDS             1703..2623
                     /locus_tag="VFJ67_07880"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET7290339.1"
                     /translation="MKVIFDPEQPIESPTSATIGNFDGVHTGHKKILSAVRETAGRKG
                     LSSCVITFHPHPQKVLQNIEIPLLMPIRERLRLLDREGMDFVACYTFTQDISKITAKD
                     FISEILVGKLKVKHLIIGPDFSFGRKREGNAELLQTMGKAYDFETRVIGPVYIDNELV
                     SSTAIRNLLKAGDARKASRFLGYNYYIEGEITEGEKRGRQIGYPTANLNTDWEMLPKT
                     GVYATRASLDDKKLDSITNIGYRPTFGSSRLLIETHIFDFNADIYGKRLRIEFVERIR
                     DEKRFESVDALVAEIRADVEKVKKILKNTE"
     gene            2663..>3505
                     /locus_tag="VFJ67_07885"
     CDS             2663..>3505
                     /locus_tag="VFJ67_07885"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7290340.1"
                     /translation="MEIKATTKIYGIFGHPVSHSLSPVMHNSAFSALGLDCVYVAFDI
                     SPEEIGKAAQAVRTLGISGINITIPHKESIIPHLDEIAPDAELTGAVNTVMNEGGKLS
                     GHNTDVGGFLRAVREDLGIEPEGSKVFLAGAGGAARAVMSAFCMNGAERIYVLNRTHD
                     KALKLASDFGRRFKKIKIEPIPFGDVKAMESGLGKADILVNSTSSGMDGQEGLDLPLD
                     KMKKTSAVYDLVYKPRETRLVKEARGLGLRASGGLGMLLYQGALSFEIWTGRQAPVDV
                     MRKAL"
BASE COUNT          906 a          924 c          953 g          722 t
ORIGIN      
        1 gggagacgca taatcataga aattgctcct gtaatccata cccatcggtg acccccttag
       61 caggagcacg atcttgcccc cgacatccag gtctctgtaa tcgtcgtatc caagctcggg
      121 agcgcttatc ccgtaccccg cgaacacgat ctctccagag acactaccgc ttgcagacag
      181 ggatagcggg aagaaatcac tgccgagccc gagctgctta ccgtgaccgt tcagagttac
      241 agttagcgag ttatcgtttc cgggaatcaa cttatcggga agaggaaatt tctggaagta
      301 tgtcccgccg tccccttgcg gcctgaggcc cgcttcctcg aaattgcggg cgatataaac
      361 ggctgccttc tcggcgccct tctccccggt catccgcccg cagagctcat ccgaagccag
      421 gtatctgaca tgagtcatga gatcgtcggg gctgatttcc tgcctcgagt aattccccgc
      481 gggctcttcc gcaagtgaag cagtcaggaa caagagcgag ataaaaagga ccttggaaca
      541 gaaccgcatg actaaataaa ttaccacacg tcaccggccg gggatgctga gcactcgata
      601 tataagtgcc gtatagccct ctgaacgttt gcagacaaaa acaatccgtg taagaatatc
      661 atccaatact ttacagggga gtaaataatg tatcccgttc tattcagggt aggagacata
      721 tcggtttctt ccttctcggt aatggttctc ctgggtttcc tcgttgcgta tattctgggg
      781 gaatcggaat tcaagagaaa ggggatcaac ggcaacctcg tggatctgct tctcatcgcc
      841 tccgtcatcg gcgggttagg cggcgccaag atcatgttcc tttaccagca ggtgcccatt
      901 acgagcttta tcgccgaccc ggtcagatac ctcgcttccg ggttcacctt tttcggcggc
      961 ctcatcggcg cggcaatatt gacatggctc gtaacccaga tgaaaaggat cagctttctg
     1021 accgtcacag acctcatgtg ccctcttctc ataatagctt atgcgatcgg cagaacaggg
     1081 tgcctgctgg tcggggacga ctacggaaac ccttcggacc tgccgtgggc aatggccttt
     1141 ccggaaggat cgccgccaac gacgctcagg gtacacccca cccagatata cgatacgatc
     1201 tgcatggcga tacttttcgc ggtgctctgg ggcatacgga agagaaactt ccccaccggc
     1261 cggatgacgg ccataacttt tattacattg ggtgtacaga ggttcttggt ggagttcctc
     1321 cgccatacca caccgagctt cattcccggt atatcccagg cccagatcat agcggtcctg
     1381 ttcgtcgctg ccgggatcgt gattctgttg aagaccaaaa ggggagcgca ggctggagcc
     1441 cattcctgag aactcaattt cgagcccggt cagagccgat tcggtcatat ataaatgctt
     1501 ttatttctgg tatgttatgg taaattaagc tcgtttaact tgaggatagt ccggttgatc
     1561 ctctctcatc cggagcgaat atcccgatat attaatttaa acggaaggaa ataaacatcg
     1621 gactgtataa acggaacacg cctgcgacat cgtgggccgg caggtttttt atccaaccgc
     1681 acgcccgtcc ggcaggactg aaatgaaggt aatattcgat cccgagcaac ccatagagag
     1741 ccccacttcc gcaacgatcg gtaacttcga cggcgtccac accggacaca agaaaatatt
     1801 gagcgctgtc agggaaaccg ccgggagaaa gggattgagc tcctgcgtta taacgtttca
     1861 tcctcatccc cagaaggtgc tccagaatat agaaattccc cttttgatgc ctatacggga
     1921 gaggctgaga ctgctcgata gggagggtat ggatttcgtc gcctgctata cgttcacgca
     1981 ggatatttcg aagatcacgg cgaaggactt catctcggag atactcgtcg gcaagctcaa
     2041 ggtcaagcac ctgatcatag gtccggactt ctcattcggc aggaagagag agggaaacgc
     2101 cgagctcctc cagacgatgg gcaaagcata cgacttcgaa acgagagtca taggtcccgt
     2161 atacatcgac aacgagcttg tcagcagcac cgcaataagg aacctcctta aagcgggcga
     2221 cgcaagaaag gcatccaggt tcctgggata taattattac atcgaaggag agattacaga
     2281 gggagaaaaa agggggagac agataggata cccgacagca aacctgaata cggactggga
     2341 aatgcttcct aaaacggggg tttacgccac aagggcaagc ctcgacgata agaagctcga
     2401 cagtatcacg aacatcggct acaggcccac attcggcagc agcaggctgc tcatcgagac
     2461 acatatattc gatttcaatg ccgacatcta cgggaagagg ctcaggatcg agtttgtcga
     2521 gaggataagg gacgagaagc ggttcgagag cgttgacgct ctcgtcgccg agatccgggc
     2581 ggacgtcgag aaggtcaaaa agatactcaa aaatacggag tgacggaata cacccccgga
     2641 tgatcccgcg cacggaccga taatggaaat aaaagcgacc accaaaattt acggcatatt
     2701 cggccacccc gtgagtcaca gcctctcgcc ggtgatgcat aacagcgctt tctccgccct
     2761 cgggctcgat tgtgtgtacg tggcgttcga tatatccccc gaagagatag gaaaggcggc
     2821 ccaggccgtc aggacactgg gaataagcgg cataaacatc acgatcccgc acaaggagag
     2881 catcattccc cacctagacg aaatcgcgcc cgacgcagag ctcacgggtg cggtcaacac
     2941 ggtaatgaac gagggaggaa aactctcggg ccacaacacc gacgtcggcg gtttcctgag
     3001 agctgtcagg gaagatctcg gcatcgagcc agaaggatcg aaggtattcc ttgcgggagc
     3061 gggaggggcc gcgcgggcgg taatgtcggc gttctgcatg aacggggccg aaaggattta
     3121 cgtcctgaac aggacgcacg ataaagcgct caagctcgcg tcagattttg ggcggaggtt
     3181 caaaaaaata aagatcgagc ctataccgtt cggcgacgtg aaagcgatgg aatcaggcct
     3241 cgggaaagct gacatactcg tcaactccac gtcgtccgga atggacggcc aggaaggatt
     3301 ggacctgccg ctcgataaaa tgaaaaagac atcagcagta tacgaccttg tttacaagcc
     3361 cagggaaaca cggcttgtga aagaggcgcg cggactgggt ctcagggctt ccggggggct
     3421 cggcatgctc ctttatcagg gggcgctcag cttcgagata tggacaggca ggcaggcccc
     3481 ggtcgatgtg atgagaaaag ccctc
//
LOCUS       DASSJH010000360         4851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_86165, whole genome shotgun sequence.
ACCESSION   DASSJH010000360 DASSJH010000000
VERSION     DASSJH010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86165"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..344)
                     /locus_tag="VFJ67_07890"
     tRNA            complement(268..344)
                     /locus_tag="VFJ67_07890"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(308..310),aa:Pro,seq:tgg)
     gene            complement(462..761)
                     /locus_tag="VFJ67_07895"
     CDS             complement(462..761)
                     /locus_tag="VFJ67_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7290341.1"
                     /translation="MADDKRWFEKHAPKMAEGWWNFYNAVEGETVLDKKTKALIAVSV
                     AVHGRCPHCTESRIKKALALGISKQEISEAIMETALLSSGTEIFWAKDVYDKYLG"
     gene            complement(830..1366)
                     /gene="msrA"
                     /locus_tag="VFJ67_07900"
     CDS             complement(830..1366)
                     /gene="msrA"
                     /locus_tag="VFJ67_07900"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009795662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7290342.1"
                     /translation="MTGNKYKKATFSGGCFWCMQPPFDNLEGVISTTVGFTGGTEKNP
                     NYHEVCYGRTGHLESIEVVYDPERVSYNDLLDVFWLNIDPTDDGGQFVDRGRHYKTAI
                     FYHDEEQKKIAEESKRKLGESGRFKSPVVTEIRPAMEFYPAEEYHQKYYEKNPIGYTH
                     YKVGSGRERYIQHMKNMK"
     gene            complement(1526..1951)
                     /locus_tag="VFJ67_07905"
     CDS             complement(1526..1951)
                     /locus_tag="VFJ67_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290343.1"
                     /translation="MKKLTALVTFLAGISGLAGIYLLFKLGNLPPGAVLIVVNAIVFR
                     GVCGVAGGILLWTGRRIGSYLALVTWCYLIIVSVLTIIGLHDKGFHFSSIVHGEVPAS
                     FGKPLLWSLAKLFLGIPIVCLVLSDLLGARKSFGSRPVA"
     gene            complement(1957..>4851)
                     /locus_tag="VFJ67_07910"
     CDS             complement(1957..>4851)
                     /locus_tag="VFJ67_07910"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7290344.1"
                     /translation="AEWYPDGESILFRSNMLSPMQRYNRLFKVSKKGGMPEVLPLPFG
                     EQGSFSPDGKKLAFEYMAPMRGVWKRYRGGMASDIWIYDFEAGSLDKLTDFPGTDTLP
                     MWHGNRIYFLSDRGSEKKLNLWVYETDTKKTRQVTDFTEFDVKWPSLGPEDIVFENDG
                     RLYLLSLADETVSEVSVDVPYDLPEARPRQKDLSENITDMAVSPNGKRALFEARGEVL
                     TVPAGEGSVRNLTSTSGIAERFPAWSPDGKYVAYFSDRSGEYELYIRLSNGEGKEERI
                     TNDGKAYRYKPVWSPDSKKLAFSDKTGRLYVTDTVGKKPKEVDKDEYENIGSYSWSPD
                     SRWIAYVINTPNNLKSVFIYDTKSGVTRKVTSDFYNDNGPVFDPEGRYLYFYSDRGFS
                     PVYGDMDDTWVYPNSTQIYAVALAKDTVSPIAPRNDEEYAPGKDAPGKSSGNNVVIDF
                     DGIEKRAVKIPVSAGNFGPLNAANGRIVYLRFPPAGSQDSPGGALQYFDLGDRREKTV
                     IPGINDYALSSSGAKVLYRAGGDNYGIIDLSEGKNPGDGKLDLAGMKAFIDPKKEWAQ
                     IFNDAWRIERDFFYDPNMHGVNWKAVRERYRKLLPYVVDREDLNFVISEMVGELNSSH
                     TYVGGGDIDEPESVPVGLLGVDFELDKKNKAYRIKKIYGGGEFDADVYSPLAEPGVNV
                     KEGDYILAVNGKPLDPAKDPWDAFQGLDSATVTLTIGPTPELKSAREVTVKVISKGDE
                     QKLRYLDRVASNMKKVDKATGGRVGYVYVPDTGLFGQNELVRQYTPQTSKEGVIIDER
                     FNGGGQVPDRFIELLNRPNLNFWAVRDFKDSKTPTVSNNGPKVMMINGWSGSGGDAFP
                     YYFRKTGIGPLVGTRTLGGLIGISGNPGLIDGGYITAPGYAFWNSHGKWEIEGHGVDP
                     DYEVEDLPANIKDSQDPQLDKAIEVITDQLDKNPPVEPRRPRYEDRSGAG"
BASE COUNT         1010 a         1414 c         1233 g         1194 t
ORIGIN      
        1 caagaagatt ctatcatatg acaacaccga aaaggaagtc ttatgcaagc cggggccgta
       61 gagggtgggg cttcgattgt agcagtcagc aagatactag gacatacgga cattaaaatg
      121 actatgcgtt acacacaccc ggacaattcc ttaaaagaag cagttgaatt actgacaaaa
      181 ggcaatattt ccgaaacaat tactgacata ttcactgaca tagcataaaa aagtaattct
      241 aaaaatgatg taacatatta aaatcattgg tcggggtgag agggatcgaa cctccgacct
      301 cggcgtccca agcgccgcgc gctaccagac tgcgctacac cccgaccggg gaaaaatgag
      361 tatacccatg acggggaaaa caacaaggcg cgaacctagc gccgttctta ttttccgaag
      421 ccggtattga tttcaagcat tataaaccgc agacaggggg attaacccag gtatttgtcg
      481 taaacgtcct tcgcccagaa tatctccgtc cccgaagaaa ggagggccgt ctccattatg
      541 gcttcgctta tctcctgttt gcttatgccg agcgcgagcg ccttctttat ccttgactcg
      601 gtgcagtgag ggcatctgcc gtgaaccgca acggatacgg ctatcaatgc cttggttttc
      661 ttgtcgagga cggtttctcc ctcgacagcg ttatagaaat tccaccaccc ttcggccatc
      721 ttcggggcgt gcttctcaaa ccatctctta tcgtcagcca tttcttaaat cctccttgga
      781 gttctcgggt gtaattatgt ccaaccctga gcgagcgggg atttcaacct tatttcatat
      841 tcttcatatg ctgtatatat ctttccctgc cggagccgac cttgtagtga gtatatccta
      901 tcgggttctt ctcataatac ttctgatgat attcctcggc gggatagaat tccatggcgg
      961 gtcttatctc ggtcactaca ggcgacttga acctgcctga ctcgcctaac ttgcgcttcg
     1021 actcctcggc aattttcttc tgctcctcgt cgtgatagaa aatcgccgtc ttgtaatgcc
     1081 ttcccctgtc gacgaactgt ccgccgtcgt cggtagggtc tatgttgagc cagaacacgt
     1141 cgaggaggtc gttgtaggag accctctccg ggtcgtatac cacctctatc gactccaggt
     1201 gtcccgttct gccgtaacag acctcgtgat aattcgggtt cttctccgtc cccccggtaa
     1261 accccaccgt cgtcgagatc actccctcga ggttgtcgaa aggcggctgc atgcaccaga
     1321 agcatccccc cgaaaaagtt gccttcttat atttattacc ggtcatttcc attgtgtcgt
     1381 ctccgtgatg atatagggac ttatttataa ctattatacg cagtgtgcgg tgtataaaaa
     1441 agccgcacta cactcagtat ttatacgccg ggaccgatgg aaaggattca ggatattttc
     1501 ataccgccgg ggaaaaaccc ggcggtcatg cgacagggcg gctgccaaat gattttcttg
     1561 ccccgaggag gtcactcagt actagacaga caatcgggat tccgagaaaa agcttggcta
     1621 gagaccaaag gagcggcttt ccgaaagaag cggggacttc cccgtgaaca atcgaagaaa
     1681 aatgaaagcc cttgtcatgg aggccgatga tggtgaggac cgaaacgatg atgagataac
     1741 accacgtaac gagagcgagg taactcccta tcctgcggcc cgtccagagc aggatccctc
     1801 ccgcaacccc gcaaacaccc ctgaacacaa tcgcgtttac tactatgagc acagcccccg
     1861 gcggaaggtt tcccagcttg aacaggagat agattcctgc gagtccggat attcccgcga
     1921 gaaatgtgac aagagcggtc agcttcttca cggcttctat ccggccccgg acctgtcctc
     1981 gtaccgggga cgccggggtt cgaccggagg gttcttatcg agctggtccg ttatcacctc
     2041 gatagccttg tcgagctgcg ggtcctgcga atcctttata ttggccggca ggtcctccac
     2101 ctcataatca gggtccaccc cgtgcccttc tatctcccac ttcccgtgcg aattccagaa
     2161 ggcatagccg ggcgcggtga tgtacccacc gtctataagg ccggggtttc cacttatgcc
     2221 tatgagaccg ccgagagtcc tcgttccgac gagcgggccg attcccgtct tcctgaagta
     2281 ataggggaaa gcgtctcctc ccgatccgga ccatccgttt atcatcatca ccttgggccc
     2341 gttgttcgat acagtgggcg ttttagaatc cttaaaatcg cgcaccgccc agaaattgag
     2401 gttgggcctg ttcagaagct ctataaacct gtccgggacc tgaccgccgc cgttgaacct
     2461 ctcgtcgatg atgacacctt ccttcgaggt ctggggcgtg tactggcgga cgagctcgtt
     2521 ctgtccgaaa aggcccgtat ccgggacgta tacgtatccg accctgccgc ccgtcgcctt
     2581 atccaccttt ttcatattag aggcgactcg gtcgaggtat ctgagcttct gctcgtcgcc
     2641 cttcgatatg acctttacag tcacctccct cgcgcttttc agctctggag tcgggccgat
     2701 tgtaagggta acggtagcgc tatccagtcc ctgaaaagcg tcccaagggt cctttgccgg
     2761 gtcgagcggc ttaccgttca cggcaagtat ataatccccc tccttcacgt taacccccgg
     2821 ctccgcgagc ggggaataca cgtcggcgtc gaattctccg ccgccgtata ttttctttat
     2881 acggtaggcc ttatttttct tgtcgagctc gaaatccacg cccagaagcc cgacaggcac
     2941 gctctcgggc tcgtcgatgt cgcccccgcc gacgtatgta tgtgaggaat tcagctctcc
     3001 caccatttcg ctgatcacga aattgaggtc ctccctgtcg acgacgtaag ggaggagctt
     3061 tctatatctt tccctgacgg ccttccagtt gaccccgtgc atattcgggt cgtagaagaa
     3121 gtccctctct atccgccacg cgtcgttaaa aatctgggcc cactccttct tcgggtcaat
     3181 aaaggccttc attcctgcga ggtcgagctt cccgtcgccc gggttcttcc cttccgagag
     3241 gtcgataatc ccgtaattat cgccgcccgc cctgtagagc accttggccc cgctcgagga
     3301 gagcgcataa tcgtttatgc caggtatgac cgtcttctcc ctcctgtccc cgaggtcgaa
     3361 gtactgaagt gctccgccgg gggagtcctg agagcccgcg ggcgggaacc tcagatagac
     3421 tattctgccg ttcgcggcgt tgagcggccc gaagttgccg gcgcttaccg gaattttgac
     3481 cgccctcttt tctattccgt cgaagtcgat cacgacatta tttcccgagc tcttaccggg
     3541 cgcgtccttt ccaggggcgt actcctcgtc gttccgcggg gctatcggag aaaccgtatc
     3601 ctttgcgaga gcgaccgcat agatctgggt cgagttcgga tatacccacg tatcgtccat
     3661 gtccccgtaa acaggggaga agccgcggtc ggagtagaag tagagatacc tcccttccgg
     3721 gtcgaagaca ggtccgttgt cgttatagaa atcgctcgtg accttgcgcg taacgccgct
     3781 cttggtgtcg tatatgaata cggatttcag attattaggc gtatttataa cgtaagcgat
     3841 ccacctgctg tcgggagacc acgagtagga gcctatattc tcatactcgt ccttgtccac
     3901 ttctttgggt ttcttcccga ccgtatccgt tacgtacagc cttccggtct tgtcgctgaa
     3961 cgcgagcttt ttgctgtcgg gagaccacac aggcttatag cggtatgcct ttccgtcgtt
     4021 cgttatcctc tcctccttcc cctccccgtt gctgagcctg atgtagagct cgtactcgcc
     4081 cgacctgtcc gagaaatagg ccacgtattt tccgtcgggc gaccacgcgg ggaaacgctc
     4141 ggcgataccc gacgtgctgg taaggttgcg gacgcttccc tccccggcag gcacagtcag
     4201 cacctccccg cgcgcttcga agagcgccct ctttccgtta ggagatacgg ccatatccgt
     4261 gatattttcc gagaggtctt tctgcctggg acgcgcctcg gggaggtcgt acgggacatc
     4321 gactgagacc tcgctcacgg tctcgtcagc gaggctcagc aggtagagcc tgccgtcgtt
     4381 ctcgaagact atgtcctcag ggccgaggct cggccacttg acgtcgaact cggtgaagtc
     4441 cgttacctgc ctcgtcttct tcgtatcggt ttcgtaaacc cagaggttca attttttctc
     4501 gctgcctcta tcggaaagga aatagatcct gttgccgtgc cacatgggga gagtgtcggt
     4561 gccggggaag tcggtcagct tatcgagcga gccggcttcg aaatcatata tccagatgtc
     4621 gctcgccatg ccgcccctgt acctcttcca cacgcccctc atgggggcca tgtattcgaa
     4681 ggccagcttc ttgccgtccg ggctgaagct cccctgctcg ccgaagggga gcgggagtac
     4741 ttcgggcatg ccgcctttct tcgatacctt gaagagtctg ttgtaccgct gcatcgggct
     4801 cagcatgttc gagcggaaca gtatgctttc accgtccgga taccactcgg c
//
LOCUS       DASSJH010000361        11556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_620792, whole genome shotgun sequence.
ACCESSION   DASSJH010000361 DASSJH010000000
VERSION     DASSJH010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620792"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VFJ67_07915"
     CDS             complement(<1..1090)
                     /locus_tag="VFJ67_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290345.1"
                     /translation="MTTILNGSVTALFAFDIGYEVALEQLSRLPSSAPVQPLSPKKRT
                     PTFLQYSKPPLVVQLGETRPILSEPGRAQVTVFDFGAASIAYQWSLPSGTGALSLADL
                     QPLSRTLYELNLEARAKEQIQVFLEENRSAIDRPELSPLVEDYYLFIIEDFDQHLSAA
                     NLLERHGSLIAQVLRFETQPLSLEQQAEALNQRLSYYTNDLVIVDWNAALVYDRDYHD
                     TVNVLELLNVELLEARYIDEQLDRRISDYQRIALKQRDSLVPLHTPNRKAIQALAELR
                     LESLVLAEKVDNALKLIGDLYLARVHALAARRLYLQDWESAISRKLDIIASLYQLLTD
                     RVRTTQSQTLELIIIALILAEILMAVFFR"
     gene            complement(1325..1627)
                     /locus_tag="VFJ67_07920"
     CDS             complement(1325..1627)
                     /locus_tag="VFJ67_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290346.1"
                     /translation="MFALLLVTGLSFGGNSFFGSTPSIAADDPAGEAPGGEMTDEATD
                     GSGAATDDAKKATPGEMPGGEMTDEATDGSGADTGTTDESTEGEMPGGDMPDDAAK"
     gene            complement(1884..3026)
                     /locus_tag="VFJ67_07925"
     CDS             complement(1884..3026)
                     /locus_tag="VFJ67_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7290347.1"
                     /translation="MKYDEINAPEIETESFAEHFEETAENGNFTEDEFIVEETPDDYD
                     EDAAMYENRADIYGRQDEQFRILNDYFRDISKEPLLTIKEEVEIPAKIKRCEDKIRQI
                     TSYLEESADGNGMHANGHLNHKMNRNRLNASLRAYSHRADKLKKRFASANLRLVISIA
                     KRYVNHGLPFLDLIQEGNIGLMKAIERYDHSKGFRFSTYASWWIYQAITRAILVHRGA
                     VRVPVYLLEVTSRVYRTKSILLKESGRNPTAEDIAEQSGVPLKYVSRIMKITNESVSL
                     DSPVDGEKRTLYDFIEDEDSVTPDRLVVDYELKESIRNSLSLLSVKEEEVLRMRFGID
                     RSEVVTLDDIGRKFKLTRERIRQIEKKALRKLSVSRLRESLKNFRT"
     gene            3461..5995
                     /gene="recG"
                     /locus_tag="VFJ67_07930"
     CDS             3461..5995
                     /gene="recG"
                     /locus_tag="VFJ67_07930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7290348.1"
                     /translation="MSNEFNEIIAALEKPLSFTSKNGFSNLDKVKDLDRLVGDLTLKA
                     LSLPLTAEESGDIEKLRHLFSSYDALGREERKGIIRESLNIIVSLKNREVSPGSGKRA
                     RETSRQPEGKRVEEPDSPAETRVRPSHDSGDEPDRVPIQFIKGVGPRLASVLKKKGIE
                     TVEDALYFFPRTYEDRRTVKKISELRPGTRETVTGKIMLAGRVRARRRSLYQITVSDG
                     TGTLTLLWFRFSEEYLRNAYKKGASVILTGDVGVGYRGELQIVHPRPEDIEVIEEGED
                     LDENHLHFNRIVPIYPLTEGLRQRRMRRIMKSVVDGYSASLSPGFPDALKRKRGVIDL
                     SAAVSRAHFPANDESIVNLSDKDSVYASPPHKTLSYHEFFLMELGLALKKRDVAELDG
                     ISFSPTGELTGKLISGLPFRLTSAQERVLSEIERDMRSGRPMNRLLQGDVGSGKTIVS
                     LLSMMKAVECGYQAVLMAPTEILAEQHLSSVMSYVKGMGLRVVFLKSGLGRGEKNAYY
                     KAIASGEAQIAVGTHALLEEKVDFRKLGLVVIDEQHRFGVMQRARLMEKGVNPDVLVM
                     TATPIPRTLALTVYGDLDVSVLDELPPGRKGIRTKIYYDHKGSREKAYEAVRKELAKG
                     RQAYVVYPMIEESESPDFKDLKYATQMAEELKNDIFPEFRVGLLHGRMKSEEKEAVMK
                     RFVSRHIDILVSTTVIEVGVDVPNATVMVVENAERFGLTQLHQLRGRVGRGGHDSYCI
                     LISGFKRSEDAEKRLSIMEETSDGFRVAEADLMIRGPGDFLGTKQSGLPEFRFADILR
                     DARILNEAREDAFLIVKEEPRLESYPGLLHEVKKKYGAIFELGGIS"
     gene            complement(6038..7300)
                     /gene="odhB"
                     /locus_tag="VFJ67_07935"
     CDS             complement(6038..7300)
                     /gene="odhB"
                     /locus_tag="VFJ67_07935"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET7290349.1"
                     /translation="MSINITVPELGESVVEATVVRWFKSVGDAVRIGDALVELETEKA
                     NFEVAAEKEGVLSGIIKGDGEDVEVGQVLGVIEEGKVGAAPGPKEAAKPAAEARIETQ
                     PRPEPAPAEKATPVAKKIAEEKKVDLGKVEGTGPSGRITKEDVEIYIEKTAEEAVPAV
                     VPEAAPARGKAPALVEAKEATLLRISRDVREERIKMSRRRRTIARRMLEATQTTAMLT
                     TFNEIDMGAVMELRRRRKETFKEKYGVSLGLNSFFVKASIGALKEFPQVNAEIDGDDM
                     IIKHYYDIGVAIGATEGLVVPVLRNADSMSFAAIERSIKDFAQRAEDNTLTLEEIMGG
                     TFTITNGGVFGSLLSTPILNPPQSGILGLHKIEDRPVTGNGSVVIRPMMYVALSYDHR
                     IVDGREAVQFLVRVKELIEDPESLLLEG"
     gene            complement(7317..10130)
                     /locus_tag="VFJ67_07940"
     CDS             complement(7317..10130)
                     /locus_tag="VFJ67_07940"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET7290350.1"
                     /translation="MDISHIFHGPNSGYVLELYERYLKDPKSVDTSTRAIFDKMRPEI
                     REEEPSEITQHDIDKIVATANLAQAIRAFGHLGADIDPLGSTPPGDPSLELSTHGLTE
                     ADLVQLPSSLIGRPISEKTSNAYDAIQELRKIYSSSVGYDYYHINSLEERKWLREAAE
                     NGTFRPPKDPINEKLLLENLTKVEVLEKFLHRIFPGKFRFSIEGVDMLVPILNEIVGI
                     AAETGIRHIFMGMAHRGRLNVMHHVMTKSYRGSLIKFKDPVLERDFRDYMGWTGDVKY
                     HEGARHAIISGREIDMQITLSPNPSHLESVNPVVEGMARAAGTTVNTSGKPKFDPEVS
                     LPIIVHGDAAFPGQGIVPETLNMCRLPGYTTGGTIHVITNNQLGYTALPNESRSTLYA
                     SDLAKGFEIPIVHVNADDPEACIECARLAFAYQNKFGKDFLIDLVGYRRHGHNEADEP
                     AFTQPEMYKIIDDHPTVREVWAEKLAGRNILDKDAADGIVNKYNEILHSEFESLTPQD
                     IPEEALSEIPPAGAAQKTITSVASEALREMNAALLKLPEGFTINPKLKRARERRENAL
                     AEIDERTIDWAMAEELAFASILADGTSIRFTGQDAERGTFSHRHAVLHDFKTGETYTP
                     LQNIPHGKAAFEILNSPLTENACIGFEYGYNIQAPDRLVIWEAQYGDFINGAQTIIDE
                     YVVSARAKWGQTPSLVLLLPHAYEGQGPDHSSGRMGRFLDSAAEVNMRIANCTTSAQY
                     FHLLRRQAGVLEIDPLPLIIMTPKSLLRNPMIFSSLRELSEGTWQPVIDDGMSTREAK
                     SVKRLIFCSGKVYIDLIASELRKQHAKDVAIARLEQLYPVPTALIQKVLGRYPNLKEV
                     VWLQEEPENMGAWMFIYPFLRKFIKGSVPLHFIGRRRNSSPAEGTASMHKVNQEALIR
                     QAYTVGKQLPNIDELGITWVKNV"
     gene            complement(10396..11274)
                     /locus_tag="VFJ67_07945"
     CDS             complement(10396..11274)
                     /locus_tag="VFJ67_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7290351.1"
                     /translation="MTMQTHIKTFAPARPIWCAGCGDFAVQGVLEKSLHKMDIPPHKL
                     MMLAGIGCSGTIQNNLPCYGYHALHGRVLPAAIGAKLANPDMTVIAAGGDGDGYAIGA
                     GHLVHTFKSNPSIMYIVMNNCNYGLTKGQASPTSPTGYHDNPESGMDSILLGLSFPSA
                     TFIARGFSGNPEQLEKLMEAALEHTESKKGFAFLEVLSPCVTYYDTHREWRARVYNVD
                     EEDGFDPADRGMAFQRCLKLKEQGMIPIGRIFKGEHPSLESLILSKTELPPAQQKVGE
                     ERHREQYKKFIDSYMG"
     gene            complement(11271..>11556)
                     /locus_tag="VFJ67_07950"
     CDS             complement(11271..>11556)
                     /locus_tag="VFJ67_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290352.1"
                     /translation="REGINTAYLQPRTVWPVLEDVLGFIDKCSRVYVVELNAQAQLAH
                     ILMHQGADPAKIINILKYDGIPFRPAELAKRVLEQEGRMVKKGKKEAKIQ"
BASE COUNT         2376 a         3137 c         3102 g         2941 t
ORIGIN      
        1 aacggaagaa cactgccatt aggatttccg ccaggatcag cgcgataata ataagctcaa
       61 gagtctggct ctgtgtcgtc cgcacacggt cggtcagcag ctgatagagg cttgcgatga
      121 tatcgagctt ccgagaaatg gcgctctccc aatcctgcag atagagtctc cgggcggcga
      181 gggcatgaac cctggccaga tagaggtccc cgatcagctt gagggcatta tcgacctttt
      241 cggccagcac cagtgattca agccgtaatt cggccaaagc ctgaatcgct ttccggttcg
      301 gcgtgtgcag cggcactagc gaatctcttt gcttgagtgc gatcctttga taatcgctga
      361 tgcgccggtc cagctgctcg tcgatgtagc gcgcttccag cagctccacg ttgagtaatt
      421 ccaggacgtt gacggtatcg tgataatcgc ggtcatagac aagcgccgca ttccaatcca
      481 cgatcaccag atcgttcgtg tagtaagaaa gcctttgatt cagagcctcg gcttgctgtt
      541 ccaagctgag cggctgagtt tcgaagcgca gcacctgtgc gatgagcgaa ccgtgccgct
      601 caagcaaatt ggcggcggac aagtgctggt caaaatcttc tataatgaat aaataataat
      661 cttctaccag gggcgacagc tcaggcctgt cgatagccga gcggttttct tccaggaata
      721 cttgaatctg ttccttcgcc cgagcttcca gattaagctc gtaaagcgtc cggctgagcg
      781 gctgcaaatc cgccaatgac aaggcccctg tgcctgaggg taatgaccat tgataagcta
      841 tactggcggc gccgaaatca aaaaccgtga cctgcgcccg ccccggctcg gaaagaatgg
      901 gtcttgtctc tcccaattgc acgaccaggg gcggcttgga atattgcaag aaagtcgggg
      961 tccttttttt aggcgagagc ggctgcaccg gcgcggacga aggtaaccgg cttaactgtt
     1021 cgagcgccac ttcataaccg atatcaaagg cgaagagggc tgtcacagag ccgttcagga
     1081 tcgtcgtcat gcgataaata ctacaattat caagcgggtt tttctataaa gtgattaagg
     1141 tattatgtga gctatacgtt tacgcgcaga accatatccc atgtcccccc cgaccataat
     1201 tacttccgcc gatcctcaga cgaaatttgt tctatacctg tatttaagtc gaaaaatcgg
     1261 aaattcgggc aaaaaaaaag gggagcggct gctccccttt catactgttc cagaatttta
     1321 ttccttattt agccgcatca tccggcatgt cgccaccggg catttcgcct tccgtggact
     1381 catctgtggt tccggtgtcg gctcccgaac cgtcggtcgc ctcgtctgtc atctcgccgc
     1441 cgggcatttc ccccggtgtg gctttcttgg catcgtccgt cgcagctccc gagccgtcgg
     1501 tcgcctcgtc cgtcatttcg ccgccgggcg cttcacctgc cgggtcgtcc gcagctatgg
     1561 acggggtgct tccgaaaaag gaatttccac cgaaggaaag tcccgttacc agcaacaaag
     1621 caaacataat agcaatcact ctcatttctt caaaccctcc tgatttttta ttgccgaccg
     1681 tatttccccg ctcggcttcc taattatatt gcaactatca taccaattaa acattattgt
     1741 aataaattgt gttaatatgg ctggttgcaa attaaatctc agattaactt gtagaagaac
     1801 agcactattt tgcgttaaaa accacatcat attgtgcgaa atcgcacatc caatcttctt
     1861 ctcaggcaag tttaaggcgg ctgttaagta cggaaattct tcagtgattc cctaagtctg
     1921 gaaacggata gcttgcggag cgcctttttt tcgatctgcc tgatcctctc tctcgtcaat
     1981 ttgaatttcc tgcctatatc gtctagcgtg acgacttcgc tccggtctat accaaaccgc
     2041 atcctcagca cctcttcttc tttgacgctc aacagcgaaa gggaattccg aatgctctct
     2101 ttcaactcgt aatccactac gagtctgtcg ggcgtaacgg agtcctcgtc ctctataaag
     2161 tcgtagagcg tccttttctc cccgtccacg ggtgagtcga ggctcaccga ctcgttcgtg
     2221 attttcatta tgcggctcac gtacttgagc ggcactccgg actgctcggc aatatcttca
     2281 gccgtcgggt tcctgcccga ttctttgagg agtattgatt ttgtccggta cacgcggctc
     2341 gttacctcga gcagataaac aggcaccctc acagctcccc tgtggacgag tatagccctc
     2401 gttattgcct ggtatatcca ccatgacgcg tatgtggaga acctgaaccc ttttgagtgg
     2461 tcgtatcttt cgatcgcctt cataagaccg atgttccctt cctggatgag gtccaggaac
     2521 gggagaccgt ggttgacgta gcgcttagcg atgcttatca cgagcctcag gttggcgctc
     2581 gcgaacctct tcttcagttt atccgccctg tgtgagtaag ctctcagtga ggcgttcagc
     2641 ctgtttctgt tcattttatg gttgagatgg ccgttagcat gcataccgtt accgtcagcg
     2701 gactcttcga gataggaagt gatttgtctg atcttatctt cgcatctctt tatcttggcg
     2761 ggtatctcca cctcttcctt gattgtaagc agcggttctt tcgaaatatc cctgaagtag
     2821 tcgttcaata tccggaactg ctcgtcctgt ctgccgtata tatcagccct gttttcgtac
     2881 atggctgcgt cttcgtcgta atcgtccggc gtttcttcaa cgatgaactc gtcttctgtg
     2941 aagtttccgt tttccgcagt ctcttcgaaa tgctcagcaa agctctcggt ctcgatttcg
     3001 ggtgcgttta tctcgtcata tttcattatc gccgtcctct catgattttt tgattctgga
     3061 tcgaactgcc gcagaaagcg aatgaacaag ccgtgcgtgg tgcaggtgat tccggctatg
     3121 ccttctcctt ttatttagaa tggagcaatg tctgtgccat aatgctcatc aatatttttt
     3181 attcgtgata cgtacgagtt acgcaattaa aagttttttg aatgagcgtg taaaccctta
     3241 tattgtgtaa aaatacactg tttgcggtgt acgaaattca accgaaatct gaaacccctg
     3301 atcgaccgtc ggggagtcac aggaaatcgt tatttgttgc gaagtacggg gcgggtgtac
     3361 ggcgattttt atatgcatgg attatttaaa aacgcactgc actccattat ctcgggcccg
     3421 accttgaatt gtctctataa gtaccagtaa aatcttatta atgtcgaatg agtttaacga
     3481 aataatcgcg gcactcgaga aaccgctcag ctttacgtcg aaaaacggct tctctaatct
     3541 ggataaagtg aaagacctcg accggctcgt aggggacctg accctaaaag ctctatctct
     3601 cccgcttaca gccgaggaat cgggggacat tgaaaaattg aggcatctct tctcatcgta
     3661 cgacgcactc ggccgggagg agaggaaagg catcatcagg gaatctttaa atatcatagt
     3721 gtcgctgaag aaccgtgaag tctctcccgg gtctggcaaa cgcgcgcgcg agacttcacg
     3781 ccagccggag ggaaagagag tggaagagcc tgattctccc gcggagaccc gggtgcgccc
     3841 ctctcacgac tccggcgacg agcctgaccg ggttccgatt caatttatca aaggcgtggg
     3901 cccgaggctc gcttccgtgc tgaagaagaa agggatagag acggtcgagg acgcgctcta
     3961 cttcttcccg aggacttacg aggacaggag gactgtaaag aaaatttcgg aattgaggcc
     4021 cgggacacgc gagaccgtaa ccgggaagat aatgcttgcg ggcagggtcc gggcgaggcg
     4081 gagaagcctc taccagataa cagtctcgga cggcacgggg acgctcactc tattatggtt
     4141 caggttcagc gaggaatatt tgaggaatgc ctataaaaaa ggggcttcag tgatcctgac
     4201 cggcgacgtt ggcgtcgggt acaggggcga gctccagata gtccacccca ggcccgagga
     4261 catcgaggtc atagaggagg gtgaggacct cgacgagaat cacctgcact ttaaccgcat
     4321 cgtccctata tatcccctga ccgagggttt aaggcagagg cgcatgagga ggataatgaa
     4381 gtcggtcgtg gacgggtact cggcttccct ctcgccggga ttccccgatg cattaaaaag
     4441 aaaaagagga gtcatcgatt taagcgcggc cgtatcgaga gcccactttc ctgcgaatga
     4501 cgagagcatc gtaaatcttt cggacaaaga ctccgtatac gcgtctcctc cccacaaaac
     4561 gctctcatac catgagtttt tcctgatgga gctcgggctc gcgcttaaga agcgcgacgt
     4621 tgcggagctc gacggaatat ccttctctcc gacaggggag cttacgggaa agctcatatc
     4681 cgggctcccg ttcaggctca cgtccgctca ggagcgcgtg ctctcggaga tagagcggga
     4741 catgcgttcg gggaggccca tgaacaggct cctccaaggg gatgtcggga gcggcaagac
     4801 gatagtttca ctcctttcca tgatgaaagc cgtcgagtgc ggatatcagg ctgtcctcat
     4861 ggccccgacg gagatactcg cggaacagca cctatcttcc gtcatgagct acgtgaaagg
     4921 gatgggtctc cgcgtcgtat tcctcaagag cggcctcggg aggggagaga agaacgccta
     4981 ttacaaggcc atcgcctcgg gtgaggcgca gatagccgtc ggcacgcacg cgctgctcga
     5041 ggaaaaggtg gatttcagga aactgggcct ggtggtaata gacgagcagc acaggttcgg
     5101 cgttatgcag agggcgaggc tcatggagaa gggggtaaac ccggacgtcc tcgtcatgac
     5161 cgcgacgcct atccccagga ccctcgccct gaccgtttac ggcgacctcg acgtatccgt
     5221 gctcgatgag ctgcccccgg gaaggaaggg aatcaggacg aagatttatt acgaccataa
     5281 agggtcccgt gaaaaggcgt acgaagccgt cagaaaggag ctcgctaagg ggaggcaggc
     5341 atatgtcgtc tatccgatga tagaggaatc cgagagccct gatttcaaag acctcaagta
     5401 cgcgacacaa atggccgagg agctcaagaa cgatatcttt cccgaattca gggtgggtct
     5461 tctccacggc aggatgaagt cggaggagaa agaggccgtg atgaagcggt tcgtctcccg
     5521 tcacatagac atactcgtct cgactaccgt gatagaagtg ggggtcgacg tgccgaacgc
     5581 gacggtaatg gtcgtcgaga acgcggagag gttcgggctc actcagctcc accagctgag
     5641 gggcagggtg ggcaggggag ggcacgactc gtactgcata ctgatctcag gcttcaagag
     5701 gtccgaggac gcggagaaga ggctttccat aatggaagaa acgtcggacg ggttcagggt
     5761 ggccgaagcc gatctcatga tcagggggcc cggggacttt cttggaacga agcagtccgg
     5821 ccttcccgaa ttcaggttcg ccgacatcct cagggacgcg aggatactga atgaggcgcg
     5881 ggaggacgcc ttccttatcg taaaggaaga gccccgtctc gaaagctatc ccggattgct
     5941 gcatgaggta aagaaaaaat acggagctat attcgagctc ggcggaatat cctgaccgtg
     6001 tattccgaaa tttacagtag ggcgcccatt ctaaccgcta tccctcgagg aggagcgatt
     6061 ccgggtcttc gatcagttcc tttaccctga cgaggaactg gaccgcctcg cgcccgtcca
     6121 caatcctgtg gtcgtagctg agcgcaacat acatcatcgg tctgataact accgaaccgt
     6181 ttcccgtgac cggcctgtct tcgatcttgt gaaggccgag gattcccgac tggggcgggt
     6241 ttaatatcgg cgtgctcaag agtgagccga atacgccgcc gttggttatg gtaaatgtgc
     6301 cgcccattat ctcttcaagc gtgagagtat tatcctcagc cctctgtgcg aagtccttta
     6361 tcgacctctc gatcgccgcg aacgacatac tgtcggcgtt cctcagcaca ggcacgacga
     6421 ggccttcggt cgcccctata gccaccccta tgtcgtaata atgcttgatt atcatgtcgt
     6481 cgccgtcaat ctcggcgttc acctggggga attccttgag tgcgcctatg gaggctttga
     6541 cgaagaacga gttcagcccg aggctgaccc cgtatttttc cttgaatgtt tccttcctcc
     6601 gcctgcggag ctccattacg gctcccatgt ctatctcgtt gaacgtggtg agcatggccg
     6661 tcgtctgagt agcctcgagc atgcgtctcg ctatcgtccg tctcctgcgg gacatcttga
     6721 tgcgctcttc cctcacgtcc ctcgatatgc ggagcaatgt cgcttctttg gcctcgacaa
     6781 gagccggggc tttgcccctt gcgggcgcgg cctctggaac cactgccggt acggcctctt
     6841 cagcggtttt ctctatgtag atttcgacgt cttccttggt gatgcgtccg ctcggtcccg
     6901 tcccttccac tttcccgagg tcgaccttct tctcttccgc tatttttttc gccaccgggg
     6961 tcgctttctc ggcaggcgcg ggctccggtc tcggctgagt ttcgatcctg gcttcggcgg
     7021 caggcttcgc ggcctccttc ggtccgggtg cggccccgac tttaccttct tctatgacgc
     7081 ccagaacctg accgacctcg acgtcctctc cgtcgccttt tataatgccg ctcaataccc
     7141 cttccttctc ggctgcgacc tcgaagttcg ctttttccgt ttcgagctcg acgagtgcgt
     7201 cccctatcct cactgcgtcg cccacggatt tgaaccatct cacgacagtc gcttctacaa
     7261 cggattcgcc aagttcgggt acggtaatgt ttatagacat attatttctc cgctatttag
     7321 acgtttttta cccacgtaat acctaattcg tcgatgttcg ggagctgctt tcctaccgtg
     7381 taagcctgcc tgatcagcgc ttcctggtta accttgtgca tcgatgcggt tccttcggca
     7441 gggctcgagt tcctgcgcct cccgatgaag tggagaggga cgctcccttt tatgaatttc
     7501 ctcaggaatg gatatatgaa catccaggcc cccatgttct cgggctcttc ctggagccat
     7561 acgacttcct tgagattggg atatctgccc agcactttct gtatgagtgc cgtcggcacc
     7621 gggtaaagct gctcgagtct cgcgatcgcg acgtcctttg catgctgttt tctaagttcg
     7681 cttgcgatga ggtcgatata gaccttgccg ctgcagaaga tgagcctttt tacggacttc
     7741 gcttccctcg tgctcatccc gtcgtctatg acgggctgcc atgtgccttc ggacagctcc
     7801 ctcaacgagg aaaatatcat cgggttccga agtaagctct tcggcgtcat gatgattaaa
     7861 ggaagcggat cgatctccag tacgcctgcc tgcctcctca gcaagtgaaa atactgcgcc
     7921 gaggtcgtgc agttggcgat acgcatgttc acttcggccg ccgagtcgag gaatctcccc
     7981 atacgtccgc tcgaatggtc gggcccctgg ccttcatacg cgtgggggag gagaaggacg
     8041 agagaagggg tctgacccca ctttgctctc gccgagacca cgtactcgtc tattatggtc
     8101 tgggcgccgt ttataaaatc cccgtattgg gcttcccata taacgagcct gtccggagcc
     8161 tgtatgttgt atccgtattc gaatcctatg cacgcgttct ccgtaagcgg gctattcagg
     8221 atctcgaaag ccgcttttcc atgcggtatg ttctgaagcg gcgtgtaggt ttctcccgtc
     8281 ttgaagtcgt ggagcacggc atgcctgtgg ctgaacgtgc cccgctcggc gtcctgtccc
     8341 gtaaagcgta tggatgtccc gtcggcgaga attgaggcga acgccagctc ttccgccatt
     8401 gcccagtcga ttgtcctttc gtcgatctcg gcaagcgcgt tttccctccg ctcccttgca
     8461 cgcttcaatt tcgggttgat cgtgaacccc tcgggtagtt tgaggagagc ggcgttcatt
     8521 tccctgagcg cttccgaagc tacggatgtg attgtctttt gtgcggcccc ggcggggggt
     8581 atttccgaga gggcctcttc cggtatgtcc tgcggggtaa gggattcgaa ctcactgtgc
     8641 agtatttcgt tgtacttgtt cacgatcccg tcggctgcgt ctttgtctaa tatgttcctc
     8701 cccgcgagct tctcggccca gacctccctc acagtcgggt ggtcgtctat tatcttgtac
     8761 atctcgggct gggtgaaagc gggctcgtcg gcttcgttgt gtccgtgccg tctgtatccg
     8821 accagatcta tgaggaagtc ctttccgaat ttgttctggt acgcgaacgc aagacgcgcg
     8881 cattcgatac acgcctcggg gtcgtccgcg ttcacatgga ctatcggtat ctcgaaaccc
     8941 ttggcgaggt cgctcgcgta gagcgtgctg cggctctcat ttggaagcgc cgtgtatccg
     9001 agctggttgt tggtgattac gtgtatcgtg ccgcccgtgg tgtatccggg gagcctgcac
     9061 atgttgagcg tttcgggtac tatgccctgg cctgggaaag cagcgtctcc gtgcacgatg
     9121 atcgggagcg agacttcggg atcgaacttc ggcttcccgc tcgtattcac cgtcgtcccg
     9181 gctgcgcgcg ccattccctc caccaccgga tttacagact ccaggtggct tgggttgggc
     9241 gacagggtga tctgcatgtc tatctctctt ccgcttatta tcgcgtggcg cgctccctcg
     9301 tgatacttga cgtcgcctgt ccagcccatg tagtccctga agtccctttc gagtaccggg
     9361 tccttgaact tgatcaggct ccctctgtat gactttgtca tcacgtggtg cattacgtta
     9421 agacggcccc tgtgagccat gcccatgaat atatggcgga tgccggtttc agccgcgatg
     9481 cccacgatct cgttgagtat ggggacgagc atgtccacgc cctctatcga gaaacggaat
     9541 ttgcccggaa atattctatg gaggaatttt tcgagcacct caactttagt gaggttttcg
     9601 aggagcagct tttcgttgat cgggtctttc ggcgggcgga aggtgccgtt ttcagcggcc
     9661 tccctgagcc attttctctc ttcgagcgaa tttatatggt agtaatcgta cccgacactc
     9721 gaagagtata ttttccggag ctcctgaata gcgtcgtagg cgtttgaggt cttttccgaa
     9781 atgggcctcc cgataaggct cgaagggagc tggacgaggt ccgcctccgt cagaccgtgg
     9841 gtcgagagct cgagggaagg gtctccgggc ggtgtgctgc ccagggggtc tatgtcggcg
     9901 ccgagatggc cgaaagccct tatagcctga gcgaggttcg ccgtcgccac gattttatct
     9961 atatcgtgct gcgtgatctc ggaaggttcc tcttcgcgga tttcgggacg catcttgtcg
    10021 aatatcgccc gtgtggacgt atcgactgat ttcggatctt tcaggtatcg ctcgtaaagc
    10081 tcaagcacat aacctgaatt aggtccgtga aaaatatgtg aaatatccat ttattcttgc
    10141 ccttttgatt agattcttca gttttccgat cggattggta ttaaagtctc aattaacgga
    10201 tagtatatta gcacagctat atttttgtgc aatctccggg catgtaatcg cccggtatta
    10261 aatagtattt cttttcgtga cgggctgcgg acgaaatttg caggatgtgc cggaatatgt
    10321 atgcacggaa acaggattaa gtccgtcgct tattccgctc tgcatattcg gcgcggatac
    10381 ataaagcagg gggtatcagc ccatgtagga atcaatgaat ttcttgtact gctccctgtg
    10441 tctttcctct cccaccttct gctgtgcggg gggaagctcg gtcttgctca ggatgagtga
    10501 ttcgagagag gggtgctccc ctttgaatat ccggccgatc ggaatcattc cctgctcctt
    10561 cagtttgaga cacctctgga atgccatacc gcggtccgcc gggtcgaatc catcttcttc
    10621 gtcgacgttg taaaccctgg cacgccattc cctgtgagtg tcatagtagg ttacgcaggg
    10681 tgaaagcacc tcgaggaatg cgaatccctt cttcgattcg gtatgctcga gcgcggcctc
    10741 catcagcttt tcgagctgct ccggattccc gctgaaaccc cgggcgatga atgtagccga
    10801 agggaagctc aagcccagga gtatcgagtc catgccagat tcagggttgt cgtgatagcc
    10861 cgtcgggctc gtgggagatg cctggccttt tgtcagcccg taattgcagt tgttcatgac
    10921 tatatacata atgctcggat tgctcttgaa cgtgtgtacc aggtggcccg ctcctattgc
    10981 gtaaccgtcc ccgtcccctc ccgctgcgat tacggtcatg tccgggtttg cgagcttggc
    11041 tcctattgcg gcgggcagta cccggccgtg gagcgcgtgg tatccgtaac aggggaggtt
    11101 gttttgtatg gtccccgagc accctatccc ggccagcatc atgagcttgt gaggaggaat
    11161 gtccattttg tgaagggatt tctcgaggac tccctggacc gcgaagtctc cgcagccggc
    11221 gcaccagatc ggccttgccg gagcgaacgt ctttatatgt gtttgcatcg tcattggatc
    11281 ttagcctcct tttttccctt cttcaccata cggccttcct gctctaagac cctcttcgca
    11341 agctctgccg gtctgaaagg aatgccgtcg tatttgagta tgttgattat tttcgccggg
    11401 tccgcgccct ggtgcatgag aatgtgcgca agctgggcct gcgcgttgag ctcgaccaca
    11461 taaaccctgc tgcacttgtc gatgaacccg agaacgtcct cgagcaccgg ccataccgtc
    11521 ctcggctgga ggtaagcggt attaatgcct tccctc
//
LOCUS       DASSJH010000362         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1055625, whole genome shotgun sequence.
ACCESSION   DASSJH010000362 DASSJH010000000
VERSION     DASSJH010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055625"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFJ67_07955"
     CDS             <1..231
                     /locus_tag="VFJ67_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET7290353.1"
                     /translation="RELHLAGATVIRGIMGYGANSKIHTAKILRLSEDLPIIIEIVDD
                     EEKIKTLIPYLEECIGNGIVTMEKVKIIKFRT"
     gene            325..768
                     /locus_tag="VFJ67_07960"
     CDS             325..768
                     /locus_tag="VFJ67_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET7290354.1"
                     /translation="MKIAVGSDERTHLTDFVIEELRKRGMEVELHGPLGGEKLGWTDV
                     AREVSERVSGGACDQGILFCWTGTGVSIAANKVPGIRAALCQDAKTAEGARKWNDANV
                     LAMSLRATSPTVAAEILDAWLGSGPEENEKRNIEKVSEIEKKYCR"
     gene            807..1829
                     /locus_tag="VFJ67_07965"
     CDS             807..1829
                     /locus_tag="VFJ67_07965"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7290355.1"
                     /translation="MKSEGYNVAIVGATGAVGEEMMSILRERNFPVNELRLLASERSE
                     GKEVEYNGSTIRIKKLDENSFGGIDIALFSAGGSRSKEFAPAAVDAGAVVVDNSSAFR
                     MEEDVPLVVPEINPHAVGDYKKRGIIANPNCTTAVTIMALKPLHDLSRIKRVVASSYQ
                     AVSGAGAQAIEELRTQTLAWASSKEIAARTFPHQIAFNLIPHIDSFLENGYTKEEMKL
                     HNETRKILEDDGISVTATTVRVPVFRAHSVSVNIETEKRITIREAREALGKFPGVKVV
                     DEPQNLKYPTPIDAAGRDECLVGRIREDYTVPAGLSFWVVGDQLRKGAALNAIQIAEL
                     LIRDYI"
     gene            1838..2377
                     /locus_tag="VFJ67_07970"
     CDS             1838..2377
                     /locus_tag="VFJ67_07970"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET7290356.1"
                     /translation="MDAVKNLMGILETRNYSELPPGIREGVLKYINRGETILVTLLDW
                     RAMYKAPKFVDSNTFFNSWFILTSRRIIIARNSSGFKRFRDIPLKDITQIFYELDNRE
                     PRVSIISPGHEDIIEFPRQASHLSTNLEEKINSAIANSRNMRDDLPGGDYIQCAGCGN
                     SIPGGGHFCPECGAKLHNP"
     gene            2497..3375
                     /gene="dapA"
                     /locus_tag="VFJ67_07975"
     CDS             2497..3375
                     /gene="dapA"
                     /locus_tag="VFJ67_07975"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7290357.1"
                     /translation="MIHGSLVAIVTPFKNSRIDEDALNKLIEFQIENGTHGIVPCGTT
                     GESPTLSHEEHEYVIELTVKAVRKRVPVVAGTGSNSTKEAIRLTRFAEQIGADAALLV
                     VPYYNKPTQEGLYLHFKQIASQVSIPIILYNIPGRSGVNMNPETIFRLARDCKNIIGV
                     KEASGSLIQASKILYLCGRDFLLYSGEDALNFPLLAIGARGFITVTANVAPRDVADLY
                     EHFAKGDYEKAKELHYRLLPLNEALFIETNPIPVKAALSMMDKVVYEYRYPLCQMADN
                     NYKELRKALTEYGILS"
     gene            3458..4078
                     /locus_tag="VFJ67_07980"
     CDS             3458..4078
                     /locus_tag="VFJ67_07980"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7290358.1"
                     /translation="MSPPDLVFEMEIWARGKTPAGVDEAGRGPLAGPVVAAAVILPER
                     FDIPGLDDSKKLTHRQRADVLERIISSALAFAVGIVHHEEIDRINILRASLRAMEIAV
                     NTLERKPDFLLIDGNQKTSLPIPQETVIKGDSRCCSIAAASIVAKVRRDELMDEYHEI
                     YPEYNFRSHKGYPTREHLEAIRKYGPCPIHRRSFRGVLQPELREYP"
     gene            complement(4082..>4256)
                     /locus_tag="VFJ67_07985"
     CDS             complement(4082..>4256)
                     /locus_tag="VFJ67_07985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7290359.1"
                     /translation="SFLDNGVRDYGYAVFGKVIDGMDVVDGIGNVQTGSKGMYGDVPQ
                     EDVVITSVKIVEQ"
BASE COUNT         1137 a         1097 c         1241 g          781 t
ORIGIN      
        1 agggagctcc atctggccgg ggccacggta atcagaggga tcatgggata cggggccaac
       61 agcaaaatac acacggcaaa gatactcaga ctctccgaag acctgccgat aatcatagaa
      121 atagtcgacg acgaggagaa aataaaaacg ctgattccgt atctcgaaga gtgtatcggg
      181 aacggtatcg tgacgatgga gaaggtcaag attataaagt tccgcacctg acgcgcgcgt
      241 gttatatttt ccttctcccc tcaagcttga agactttacg caacacattt aaaatactta
      301 ataaagagaa acaaggacgt ttgcatgaag atagccgtag gaagcgacga gcggacacac
      361 ctgaccgact ttgtcataga ggagcttcgg aaaaggggga tggaagtaga gctccacgga
      421 cccctcgggg gagagaagct cgggtggaca gacgtcgccc gtgaggtctc ggagagagtg
      481 tcaggagggg cgtgcgatca gggtatactc ttctgctgga cggggactgg cgtgagcatc
      541 gccgccaaca aagtgcccgg aataagagct gcgctctgcc aggacgcaaa gaccgcggaa
      601 ggggcaagga aatggaacga cgccaacgtg ctcgccatga gcctgagggc gacgtcaccg
      661 acagtggcgg ccgagatact ggatgcatgg ctcggctcgg ggcctgaaga gaacgagaag
      721 aggaacatcg agaaagtaag cgagatcgaa aaaaaatact gccggtgaca tataccggga
      781 gaacgtgata atatccggag gaagaaatga agagcgaagg ttataacgtc gcgatagtcg
      841 gagcgacggg agctgtcgga gaggagatga tgagcatact cagggaaagg aatttccctg
      901 taaacgagct gagactcctc gcgtcagaaa ggtcggaagg aaaggaagtc gaatataacg
      961 ggagcaccat taggataaaa aagctggacg agaattcctt cggcggcata gacatagccc
     1021 ttttttccgc cggagggtcg aggagcaagg aattcgcgcc cgctgcggtc gatgcggggg
     1081 cggtagtcgt cgataacagc tccgccttcc ggatggagga agacgtcccg ctcgttgtgc
     1141 ccgaaataaa ccctcacgcc gtcggggact acaagaagag agggataatc gccaatccga
     1201 actgcacgac ggcggtcacc atcatggcgc tcaagccgct ccacgacctg agcaggataa
     1261 agagggtggt cgcgtcgagc tatcaggcag tgtccggcgc aggggcgcag gccatagaag
     1321 agctccgcac acagacgctc gcgtgggcga gctcaaaaga aatagccgcc aggacgttcc
     1381 cgcaccagat cgccttcaac ctcatccctc acatcgacag ctttctcgag aacgggtata
     1441 cgaaggagga gatgaagctc cacaacgaaa cacgcaagat actggaagac gacggcatct
     1501 ccgtgaccgc gacgaccgtg agggtgcctg tattccgggc gcactcggtt tccgtaaaca
     1561 tagagaccga aaagaggata acgatcaggg aggcgaggga ggccctcggg aaattccccg
     1621 gggtgaaggt ggtcgatgag ccccagaacc tcaaataccc tacgcccata gacgccgcgg
     1681 gccgggacga atgtctcgtg gggaggataa gggaggacta taccgtgcct gcaggtctga
     1741 gcttttgggt cgtgggagat cagcttagga agggcgccgc tctgaatgcg atacagatcg
     1801 cggagcttct tatcagggac tatatctgat gccgattatg gacgcggtaa agaacttgat
     1861 ggggatactg gagacgcgga actactccga gctccctccg ggcataagag agggcgtcct
     1921 taaatacatc aacagagggg agaccatact cgtcacgctg ctcgactgga gggcgatgta
     1981 taaggcgccc aagttcgtcg acagcaacac gttcttcaat tcctggttca tcctgacgag
     2041 caggagaata ataatcgcaa ggaactcttc cgggttcaag aggttcagag acatcccgct
     2101 caaggacata acgcagattt tctacgagct cgataatcgg gagccgaggg tttcgatcat
     2161 ttcgcccgga catgaggaca tcatagaatt cccgaggcag gcgtcccacc tctcgacgaa
     2221 tctcgaagaa aaaataaact cggcgatagc caactccaga aatatgagag atgatctgcc
     2281 gggcggggac tatatacaat gcgcgggatg cgggaacagt attccggggg gcggtcattt
     2341 ctgcccggag tgcggagcga agctgcacaa cccctaggga gtccgatgcc ccgggcgcgc
     2401 tgaagcgtca gcgggctttc ctgtatcgga tcgattcctt gatttgcgcc tgagcttcaa
     2461 tataatgaaa tgcagccagc agaaggagac tatcaaatga ttcacggctc attagtagct
     2521 attgtcacac cgttcaagaa cagcagaatc gacgaagacg ctctcaacaa actaatagaa
     2581 ttccagattg aaaacggcac gcacggcatc gtgccctgcg gaacgacagg agaatccccg
     2641 acgctttctc acgaggagca cgagtacgtc atcgagctta ccgtcaaagc ggtgagaaag
     2701 agggtcccgg tggtcgcggg gacgggctcc aacagtacta aggaggctat caggctcacg
     2761 aggttcgcgg agcagatagg ggccgacgcc gcccttctag tagtgcctta ctataacaag
     2821 ccgacgcagg aagggcttta tctgcacttc aaacagatag cttcacaggt gagcattccg
     2881 attatcctat acaacatccc cgggcgttcg ggcgtcaata tgaacccgga aacgatattc
     2941 agactcgcca gggactgcaa aaatataata ggcgtgaagg aggcctcggg ctctctcatt
     3001 caggcgagca agattctcta cctctgcggc agggatttcc tcctttattc gggagaggac
     3061 gcgcttaact tcccgctcct cgctataggc gcgagaggtt tcatcacggt tacggccaac
     3121 gtcgcgccca gggacgtggc cgacctctac gagcactttg cgaaggggga ctacgaaaaa
     3181 gccaaggagc tccactacag gctccttcct cttaacgaag cgctattcat agaaacgaac
     3241 ccgattcccg ttaaagccgc gctcagcatg atggacaagg tagtctacga atacagatac
     3301 ccgctctgcc agatggcgga taataattac aaagagctca ggaaagcgct gaccgaatac
     3361 ggcatcttga gctgagaata aattgaggga tgcttgataa gcgaggcttc ccggtcgaat
     3421 ctgaatccaa ggggattgcg gtcaggagca tgtgagaatg agtcctcccg atctcgtgtt
     3481 tgaaatggaa atctgggcaa gggggaaaac ccccgcgggc gtcgatgaag cgggccgcgg
     3541 acctctcgcg ggtcccgtag tcgcggcggc cgtaatcctg cctgagagat tcgacatacc
     3601 gggcctcgac gattccaaaa agcttacaca ccggcaaaga gcggacgtcc tcgaacggat
     3661 aatttcctcg gccctggcct tcgccgtcgg aatagtccac cacgaggaaa tagacaggat
     3721 aaatattctc agggcttcgc tccgggccat ggagattgcc gtcaacacgc tcgaaaggaa
     3781 acccgacttc ctgctcatag acggcaatca gaagacctcg ctcccgatcc cgcaggagac
     3841 ggtcataaaa ggggactcca ggtgctgctc catcgcagcg gcttcgatag tggccaaggt
     3901 caggagggac gagctcatgg acgaatacca cgagatctac cccgaatata atttcaggtc
     3961 gcataagggg tacccgacga gagagcacct cgaagctata aggaaatacg gcccctgccc
     4021 tatccacagg aggagtttca ggggagttct acagccggaa ttgcgggaat acccgtaggc
     4081 tctactgttc cacgatcttt acggaagtaa tgacgacgtc ttcctgaggg acgtccccgt
     4141 acattccttt gctgccggtc tgcacattcc cgatcccgtc aacgacgtcc ataccgtcta
     4201 tgaccttgcc gaatacggcg tatccgtaat ccctgacgcc gttatcgagg aagctg
//
LOCUS       DASSJH010000363        12373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_737996, whole genome shotgun sequence.
ACCESSION   DASSJH010000363 DASSJH010000000
VERSION     DASSJH010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737996"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFJ67_07990"
     CDS             complement(<1..395)
                     /locus_tag="VFJ67_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290360.1"
                     /translation="MRDRKKPDKRKFVLELLSPYKKYIALLLVLSLFAAALDGISIGM
                     LVPLLSGIQQIKNYGQLPEILQSIIKIFAAYPIEKQILLSLTLVVLALVLKNLVLAVF
                     YYLAYWLTARATEGLRGRVVDTLMTVGVG"
     gene            complement(508..837)
                     /locus_tag="VFJ67_07995"
     CDS             complement(508..837)
                     /locus_tag="VFJ67_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290361.1"
                     /translation="MNGDREEEKLLRAVVILNAKVLGLILGITLGLVIFIATNWLVLK
                     GPQPDPSGQYVLGPHMQLLSQFFIGYRVSFWGSIIGFIYGFAVGTIGGALIGWIYNRI
                     VLLRQRY"
     gene            complement(848..2710)
                     /locus_tag="VFJ67_08000"
     CDS             complement(848..2710)
                     /locus_tag="VFJ67_08000"
                     /inference="COORDINATES: protein motif:HMM:NF005547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7290362.1"
                     /translation="MTENRNDKKAVIIGAGPAGLTAAYELSKAGIRSTVIEKDGVVGG
                     LARTVNYKGYRFDIGGHRFFTKVSAVERMWREVLGSDFLKRERLSRIYYNNRFFYYPF
                     RLTNALYGLGIRNSILIALSYLKSQLSPELPEDTFDKWVSNRFGRRLYGLFFKTYTEK
                     VWGTPCSEISAEWAAQRIKGLSLMQALRNALLKDNDSGKNDTIKTLIDSFDYPRLGPG
                     MMWEAVADDLSRGGNELLMNTDVKKIHVANGRVEKIETTGTGGNEFVYGTDFISSMPV
                     RELIQKIAPAPPEGVVRAAGGLNYRDFLTVSLVVNKPDVFPDNWIYIHEPGVKLGRIQ
                     NFKNWSGDMVPDPGKTCLGLEYFCFEGDGLWSMSDPELIELAKRELESINLVDTSLVE
                     DGAVVRMPKAYPVYDTGYREALGTIREYLKGISNLQLVGRNGMHKYNNQDHSMLTAML
                     AARNVLGADYDLWMVNADQEYHEEAVLRGKELYEFREIAGTQPSVPEPVAPGRAAALD
                     QAIIRAFARMDKFAFAVSMGTVSGLAVLLATLILLLKGGQVVGPTLSLLGQYFTGYSV
                     TPGGALIGFGYAFLWGFIIGWLFAYLRNFATGLYIFRISKERDLSAFKNFIDYI"
     gene            complement(2807..4624)
                     /locus_tag="VFJ67_08005"
     CDS             complement(2807..4624)
                     /locus_tag="VFJ67_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET7290363.1"
                     /translation="MSRPSKLTTIHEQSRSYNKIKSKEIIDHVYREFVPYYEIPDFPM
                     QIMAPSGALMEIVDDPAVIVGTAVLNKTSKRVAVIAQLMPSNDAERARLNYGLVKADG
                     YALSYNMMSYAEENGLMLHTYIDTIGGDPFEYSAGKLQSWLISDCQAKMISLKTKSIS
                     TVIGSGGSGGAIALQLAHKRFMLSRAEFSVITAEGCSAILFRSADNIEEALEVLQPTS
                     EFMLKYGIVDKVIKEPALESSDYKRKVLVNIEKTILAATDELEKSDIRYLHSDLVSKI
                     QECGEVEKREHTYHAIARKIKSWLPHYSFSRTATSEVSHMQIALYGAEPHFCNDEKDQ
                     DGKIVRAGCRKHFAKEEFQKNYYACPYCEKPNPIGSHDYLDLLLNSDSFHELHSDLSV
                     DDIDARFSFHDYSDTRKKNAGRIDSKDSLVVGYGDMFDLPLAVAVSEFRFMGGSMGAV
                     FGEKMKLIAEFAVKENLPLICVTASGGARMQEGTVALYQMAKTISAILKVKEAGLPFI
                     SVLGHPTTGGALASYAVQGDFIIAEKKATVAFAGDRVVKLTSGGRGVEPNTMTSEFFS
                     KKGGIHLVTERSQLKSSIAGVLRLTPWYWRHKNRQSPTD"
     gene            complement(4794..5819)
                     /locus_tag="VFJ67_08010"
     CDS             complement(4794..5819)
                     /locus_tag="VFJ67_08010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7290364.1"
                     /translation="MKFGIGYFSLQSPPFNPRSHKDLYAEMLDEIGMAEEMGFDSAWL
                     TEHHFLKDGYCPSLMVTAAAIAARTKKIRIGTGVLLMPLHDPVRVAEDTAVVDLISGG
                     RLILGIGLGYRPEEFAGFGRSLRERRGRMEESLEILNGSWRDEPFTFDGKYYKLENVN
                     VTPKPVQRPIPIWIGAFTEPAIRRAARIGAPLYVPAIGIIPIVKYLFDMHSSLLKEYG
                     RNPGDFEKPLVREIYISDKKSDKIWEKIKENVTYTAKGYASWGSMVDREGNLLSDPDD
                     PVLYDIAREQSIIGTPEECIETVKRYKDELPVNNLICRFKFPGISHDEAVRSMKLFVE
                     KVLPAVS"
     gene            complement(5998..6321)
                     /gene="erpA"
                     /locus_tag="VFJ67_08015"
     CDS             complement(5998..6321)
                     /gene="erpA"
                     /locus_tag="VFJ67_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET7290365.1"
                     /translation="MFTVTEKAAEEIKKLLSEENVPNAVLRVRVVPGGCSGFSYEMGF
                     DDETEDSDQLFENGGVKVAIDELSFTYLQGAVLDYKDGLNGTGFSINNPNAKGSCGCG
                     QSFNA"
     gene            complement(6354..6839)
                     /locus_tag="VFJ67_08020"
     CDS             complement(6354..6839)
                     /locus_tag="VFJ67_08020"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HET7290366.1"
                     /translation="MQTALKFIYILSVCFWIGSIFFFSFFAAPSIFKVLPRETAGNVV
                     SDIFPKYYLVAYICGGAAIITTILLRMLSGQGGSILFIIRLSALAVMLALAVYAGAVL
                     RPQAVDARTEMRSLTEDSPNYPVSQEKFRKLHSRSAIINSAVFLLGIAILFINAYTNK
                     E"
     gene            complement(6842..7204)
                     /locus_tag="VFJ67_08025"
     CDS             complement(6842..7204)
                     /locus_tag="VFJ67_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290367.1"
                     /translation="MKTVSLDQICELYMDYPMSADEIYHDKTVKTTVKVAGVRKISLI
                     CAGFPEGSFTMEVVTKEGPILECYCNPPTFISVLESTPPGSSLAITGTYKSMTGSFSQ
                     NPDEEEQCKVTMHSCSFK"
     gene            7472..8605
                     /locus_tag="VFJ67_08030"
     CDS             7472..8605
                     /locus_tag="VFJ67_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET7290368.1"
                     /translation="MKKNTCLIIDKSEDNADLYYRTGFFVPDPVIYVEHNGKKTLVLS
                     DLEIDRGRKEATVDRVLSLTDLHKRLAADKVKKIKLLDIAELVLRDMKIKKVTVPGRF
                     SIKYADGLRKRGYRVIPRDEEPFFEGRLRKTPREIGMIRDSMRETVRAMDYAIKIIAS
                     SEIRKKRLYYKGALLTSERVKAEINAMLSGKGFTASHTIVACGAHSSMPHHSGEGPIY
                     ADKPVVIDIFPRSQSSGYFGDMTRTVVRGEPSPALVKMYDTVLRGQKLAIGMIRAGVK
                     VRDIHSAVVAYFKSRGFETGMLDGKMQGFIHSTGHGLGLEIHEPPRISLGDEVLEEGN
                     VVTVEPGLYYEKTGGVRIEDVVVVGKGGSANLTRYPKKLIAKA"
     gene            complement(8633..9223)
                     /locus_tag="VFJ67_08035"
     CDS             complement(8633..9223)
                     /locus_tag="VFJ67_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290369.1"
                     /translation="MADNSGKTKGSILSNAKEYIISAHGESAWKKIVNNLPEPDSAIL
                     EGDLVSTDWYPAPLLNRLLNTYDVLHGKGDFLSIVPIAEYIAQKDLGPMLDALVNLKK
                     PAVILKSAPSLWGRYFDSGMLELELLDMEKKYSVLYLDEIADEEKASGVAVFNFAIPE
                     WFKTGLTMAGANSVKIIQTACRYKNDKICRFEVWWD"
     gene            9345..10115
                     /gene="mazG"
                     /locus_tag="VFJ67_08040"
     CDS             9345..10115
                     /gene="mazG"
                     /locus_tag="VFJ67_08040"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET7290370.1"
                     /translation="MKKSFDDLVKLSIHLRSPGGCPWDREQTLDTLKSYIIEEAYEVV
                     QAIESRDTDELIEELGDLFYEVLFASQIAEESLGFGIDDVIDRLHNKLVRRHPHVFGE
                     EKAANAEEAVKRWHSEKLKEKSRKRRLLHIPRTMPSLLRAQRVGEKASQVGFDWSGAE
                     EVVSKVREELSELEEEIKSRNASGIEKEWGDLVFSLVNLGRHLNLDAEGAAHKAIDSF
                     IERFGRIEDKAESRGKELSELSLTEMDELWEEVKIEHK"
     gene            complement(10155..11366)
                     /locus_tag="VFJ67_08045"
     CDS             complement(10155..11366)
                     /locus_tag="VFJ67_08045"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HET7290371.1"
                     /translation="MKYVILQGDGMPDHPLPELGGKTPLEAARTPNLDRIAKSAIKFG
                     MVKTIPDDLPPGSDVGNLTVLGYNPHLYYTGRSPLEAASIGVPLRDGDVTLRCNLVTL
                     VSGQGGLVMDDYSAGHITTEEARNIILDLKKELDEKNLTFFPGVSYRHLLVWAGGNMD
                     IHTTPPHDISGKGIDAYIPSGDGTEKLNELIERSREILKDHPVNKKRVAEGKNPATSI
                     WLWGQGVAPAMPSFRELYGLTGSVISAVDLVKGIGHYAKLEVIDVPGATGYLDTNYEG
                     KVDYALDSLNEVDLTMIHIESTDETGHVGKAELKIQAIEDFDGRVVGRVLEGIKRFRD
                     YRILVMSDHPTPIDLRTHVNEPVPFAIYSSEDVSIKNDSFVYTERSAATSPVFIPEGW
                     RLLGMFVGREI"
     gene            complement(11461..>12373)
                     /gene="lipA"
                     /locus_tag="VFJ67_08050"
     CDS             complement(11461..>12373)
                     /gene="lipA"
                     /locus_tag="VFJ67_08050"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET7290372.1"
                     /translation="PDWIKAQLPRGPNYTHLKNLMRTLNLHTVCEEARCPNIGECWGA
                     GTLTFMILGDTCTRSCGFCHVKTGRGGELDYEEPARVAEAVRDLTRNNARITHIVITS
                     VNRDDRNYESACIFAETIRKVRAENPEVKIEVLIPDFKGERGALGEVLNARPDVLNHN
                     IETVPRLYRLPQMTPSGKRRSVRPQANYDWSLRLLSESKTLGHEGMLTKSGIMVGLGE
                     EMDEVIETLGDLKKAGCDIVTIGQYLQPTYDHLSVSKFYHPMEFESLKTYGENVIGIP
                     HVESGPLVRSSYHAEKQVVKMSGHALS"
BASE COUNT         2683 a         3430 c         3225 g         3035 t
ORIGIN      
        1 aatccgaccc cgaccgtcat gagggtatcc accacgcgtc ccctgaggcc ttcggtcgcc
       61 ctcgctgtca gccagtaggc gaggtaatag aatacagcca gtacgaggtt ctttagaacg
      121 agcgccagca ctacaagtgt gagcgagagc agtatctgtt tttcgatcgg atatgccgcg
      181 aagattttga tgatcgattg aaggatttca gggagctgcc cgtaattctt tatctgctgg
      241 attccgctca ggagcgggac cagcatccct atggagatac cgtccaaagc ggccgcgaat
      301 aatgataata cgaggaggag ggcgatatat ttcttatacg ggctcagtaa ttcgagcacg
      361 aattttcttt tgtcaggctt tttccggtcc ctcaacgcag catatcccgc atctcgattt
      421 tctctatacg gatcgaatga tattttactc gaatttcagg ccgttttaac cgttcccgga
      481 gggcgtcagg cagtgaaacg gatggaatca gtacctctgt cttaaaagaa cgatacggtt
      541 gtatatccat cctatgaggg ccccccctat ggttccgact gcgaacccgt atatgaaccc
      601 gattatgctg ccccagaacg ataccctgta gcctataaaa aactgactga ggagctgcat
      661 gtgagggccg aggacatact gccccgaagg gtcgggctga ggtcctttga gcacgagcca
      721 gttggtggct atgaaaatga cgagccccag tgtgatgccg agtatcagac ccagcacctt
      781 agcgttcagt atgaccaccg cccgaagcag tttctcttct tccctgtcgc cgttcataac
      841 tcaggcctta aatataatct ataaaattct taaatgcaga taggtctctt tccttgctga
      901 tcctgaagat atatagaccc gtagcgaaat tcctcagata cgcgaacagc catccgataa
      961 tgaagcccca cagaaaggcg tagccgaacc cgatgagagc gccgccgggg gttacggagt
     1021 agccggtgaa atattgaccg aggaggctga gggtcggacc cacgacctgc cctcctttca
     1081 ggagcaggat aagcgtcgcg aggagcaccg cgagtcccga aacggtaccc attgagacag
     1141 cgaaagcgaa cttgtccatg cgggcgaagg ccctgataat tgcctgatcg agcgccgcgg
     1201 ctctcccggg cgccacaggt tcgggaacac tcggctgtgt cccggcgatc tctctgaatt
     1261 catagagctc ttttccgcgt aagaccgcct cctcgtggta ttcctggtcg gcgttaacca
     1321 tccagaggtc gtaatctgct ccgagtacgt tcctcgctgc gagcatcgct gtgagcatcg
     1381 agtggtcttg gttgttatat ttgtgcatgc cgttccggcc gaccagttga aggttggata
     1441 tccctttcag atactcgcgt atagtcccga gcgcctcacg gtaacccgtg tcgtagaccg
     1501 gataggcttt cggcatcctc acgaccgctc cgtcctctac gagcgaagtg tccacgaggt
     1561 ttatgctttc gagctcccgc ttcgcgagct ctataagctc cgggtcgctc atagaccaga
     1621 gcccgtcgcc ctcgaaacag aagtactcga gtccgaggca cgtcttcccc ggatccggaa
     1681 ccatgtcccc gctccagttc ttgaaattct gtatcctgcc cagcttcact ccgggctcgt
     1741 gtatgtaaat ccagttgtcg ggaaaaacgt ccggcttatt gacgacgagc gataccgtaa
     1801 gaaagtccct gtaattgagg cccccggcgg ccctgacgac tccctctggc ggtgccggag
     1861 cgatcttctg tataagctcc ctcacaggca tactgcttat aaaatcggtc ccgtagacga
     1921 attcgtttcc ccctgttccc gtagtttcga tcttctccac cctaccgttg gcgacgtgga
     1981 tctttttgac gtccgtattc ataagcagct cgtttccgcc gcgcgagagg tcatcggcaa
     2041 ccgcctccca catcattccg ggcccgagcc tcggataatc gaagctgtct ataagcgtct
     2101 tgatagtatc gttctttccg gagtcgttgt ccttcagcag cgcgttcctg agggcctgca
     2161 tgagggagag ccccttgatc ctctgggcgg cccattcggc gcttatctcg ctgcagggcg
     2221 tcccccacac cttttccgtg taggtcttga agaagaggcc gtagagacgc cgtccgaagc
     2281 ggttcgagac ccacttatcg aacgtatcct cgggcagctc cggggacagc tgggatttca
     2341 ggtagctgag cgctatgagt atgctgttcc ggatgccgag cccatagaga gcgttggtca
     2401 ggcggaaagg gtagtaaaaa aaacggttgt tgtagtatat acgcgagagc ctctcccgct
     2461 tgaggaagtc acttcccaga acctcccgcc acattctttc gacggcgctt acctttgtaa
     2521 agaaacggtg tcccccgata tcgaagcggt agcccttata attaacagtc ctggccagcc
     2581 ccccgaccac gccgtccttc tctataacag tggatcttat cccggccttg gaaagctcgt
     2641 aagcggccgt gagcccggcc ggccctgctc ctattatcac tgccttctta tcgtttctgt
     2701 tttcagtcac gtagtcgcgg ctcatatttt taccatatcg aaaatatcgg gctttgtcac
     2761 tcgggcgaga actctatctc cgggccgggt atccggaacc ccggtttcaa tcggtcgggg
     2821 attgtctgtt tttgtgtctc cagtaccagg gcgtgagcct caataccccc gcgatcgagg
     2881 atttaagctg gctgcgctct gttaccaggt gaatccctcc ctttttcgag aagaattccg
     2941 aagtcatcgt gttgggctcg acgcccctcc cgccgctcgt cagctttacg accctgtctc
     3001 cggcgaacgc gaccgtggct ttcttttcag cgattatgaa atccccctga accgcgtagc
     3061 tggcgagcgc cccgcctgtg gtgggatgtc cgaggacgga tatgaaaggg agacccgcct
     3121 ccttcacctt gaggattgcg gagatcgtct tcgccatctg ataaagcgct accgtccctt
     3181 cctgcatcct ggcccctcct gaagcagtca cgcatataag gggcaggttt tcctttacgg
     3241 cgaactccgc tatgagcttc attttttcac cgaatacggc ccccatggag ccgcccatga
     3301 acctgaattc actgacggcg accgcaaggg gaagatcgaa catatcacca tacccgacca
     3361 cgagcgagtc cttggaatcg atacggcctg cgttcttctt acgcgtatcg ctgtaatcgt
     3421 ggaagctgaa cctcgcgtct atatcgtcca cgctgagatc ggaatgcagc tcgtggaagg
     3481 aatcgctgtt taacaggagg tcgaggtagt cgtgggagcc tatcgggttc ggcttctcgc
     3541 agtaagggca cgcgtagtag tttttctgga attcctcttt ggcgaagtgt ttgcggcacc
     3601 ccgctctcac gatttttccg tcctggtcct tttcgtcgtt acagaagtgg ggctcggctc
     3661 cgtatagcgc gatctgcata tgcgaaacct cggacgtcgc tgtcctggag aaagaataat
     3721 gcggaagcca gctttttatt ttcctcgcga ttgcatgata cgtatgctcc cgcttctcca
     3781 cctctccgca ctcctggatt ttactgacga ggtcgctgtg caggtacctg atatccgact
     3841 tttcaagctc atccgtcgct gcaagtattg tcttctcgat gttcacaagc acctttcttt
     3901 tgtagtccga gctctcgagc gcgggctcct taatgacctt gtcaacgatg ccgtatttga
     3961 gcatgaactc cgaagtaggc tggaggactt cgagagcctc ctctatattg tcggcgctcc
     4021 tgaataggat cgcagagcag ccctcggccg taattactga gaactcggcg cgggaaagca
     4081 taaacctctt gtgcgccagc tgcaaggcta ttgcgccacc gctgccaccg ctgccaatga
     4141 ccgtcgaaat ggatttagtc ttaagcgaga tcatcttcgc ctggcagtcg gagatgagcc
     4201 acgactgaag cttcccggct gagtattcaa aagggtctcc gcctatcgtg tcgatgtacg
     4261 tgtggagcat gaggccgttc tcttccgcgt agctcatcat gttgtacgaa agtgcatatc
     4321 cgtccgcttt tacgagcccg taattgagcc tggccctctc ggcgtcgttg gaaggcatca
     4381 gctgagctat cacggcgacc ctcttgctcg tcttgttcag cacggctgtc cccactatga
     4441 ccgcagggtc gtccacgatc tccataagcg ctccggacgg cgccattatc tgcatcggaa
     4501 aatcagggat ctcgtaataa ggcacgaatt ccctgtagac gtgatcgatg atctctttcg
     4561 acttgatctt gttataagac ctcgattgct cgtgtatcgt cgtcagcttg gaaggacggc
     4621 tcatctatat gcttctccgt gaatattgaa acaatttacc tgttatcggg ctcaaagggg
     4681 aaatattata accatagacg aatcgattgg atacaccgcg cagcaggctg tggcaagcct
     4741 cttccggagc ccggtttcga gccccggctt tttatctcgg gctaaagccg ttattacgat
     4801 acggccggta acaccttctc cacgaacaat ttcatcgacc tcacggcctc atcatggctg
     4861 atgccgggga acttaaaacg gcatattaga ttattcaccg ggagctcgtc cttatagcgc
     4921 tttacggtct cgatgcattc ttcgggagtg cctattatag actgctccct cgcaatatcg
     4981 taaaggaccg gatcgtccgg gtctgaaagc aggttcccct cgcggtcgac catcgagccc
     5041 caggacgcgt atcccttcgc cgtatatgta acgttctcct ttattttttc ccagatttta
     5101 tcgctctttt tgtccgatat atagatttcc ctgacgagcg gcttttcaaa gtcgccggga
     5161 ttcctcccgt actccttcag cagcgagctg tgcatgtcga agaggtattt gactatcggt
     5221 atgatgccta tcgccggtac gtaaagcggc gcccctatcc tggccgccct cctgatcgcg
     5281 ggctctgtaa aagcgcctat ccagatcggg atcggtctct gtacgggctt gggcgtgacg
     5341 ttaacatttt cgagcttata gtatttcccg tcgaaggtaa acggctcatc ccgccagctg
     5401 ccgttcaata tctcgagact ttcctccatg cgccccctcc tttccctgag cgacctcccg
     5461 aaccccgcga actcctcggg cctgtatccg aggccgatgc ccagtatcag ccttcctccg
     5521 gaaatgagat cgacgacagc ggtgtcttcg gcaacgcgga ccgggtcgtg gagcggcatg
     5581 agcaggactc ctgttcctat gcgtatcttt ttggtcctgg cagcgatggc agcggcggta
     5641 accataagcg aagggcaata cccgtccttt agaaaatggt gttccgtaag ccacgccgaa
     5701 tcgaaaccca tctcctcggc catgccgatc tcgtcaagca tctcggcgta caagtctttg
     5761 tgcgacctcg ggttaaacgg cggcgactga agactgaaat agcctattcc gaatttcata
     5821 gcgatgatcc ccccttcagt gttttatgcg tgatggattt atatcaatgc gccgtagcgg
     5881 tactgctcac ggtctattat aacctgaaag gacttgtatt tataagatgg gaaaaataaa
     5941 agggccgggt agacccaacc tcggcgcccg gccctcgttt tattctgtag ttttgtctta
     6001 tgcgttgaag gactgtccgc agccgcagga acccttcgcg ttgggattgt ttatggagaa
     6061 ccccgtcccg ttgagcccgt ccttgtaatc gagcaccgcg ccctggaggt acgtgaagct
     6121 aagctcgtcg attgcgactt taacgccccc gttttcgaaa agctggtccg aatcctctgt
     6181 ctcgtcgtca aaccccattt cgtatgaaaa tccggaacat ccgcccggca ctaccctgac
     6241 cctcaacacc gcgttcggaa cattttcttc cgataacagc ttctttatct cttcagcagc
     6301 tttttcagtt actgtaaaca ttactaaccg cctccctgta tgaataaaat ccactactct
     6361 ttattagtat atgcattgat gaacaatatg gcaattccca gaagaaagac cgcggaattg
     6421 attattgccg accgcgagtg cagtttcctg aatttttcct gtgataccgg gtaattagga
     6481 gagtcctcgg tcaggctcct catctccgtc ctggcatcga ccgcctgcgg tctcagcacc
     6541 gcgcccgcat acacggcgag cgccagcatg accgcaagcg cgctcagcct gataatgaac
     6601 aatatcgacc caccctgccc ggagagcatc ctgagcaata tggtcgtaat aatggccgcc
     6661 cccccgcata tgtatgcgac gagatagtat ttcgggaata tatccgatac cacattcccc
     6721 gcggtttctc tcgggagcac cttaaagatg ctcggtgccg cgaagaacga gaagaagaaa
     6781 atactcccta tccagaaaca aaccgagagg atatatatga acttgagcgc cgtctgcatc
     6841 gtcacttgaa actgcagcta tgcatcgtta ccttgcactg ctcttcctca tccgggttct
     6901 gggaaaaaga gcccgtcatc gacttgtaag tgccggtgat agcgaggctg ctacccggag
     6961 gagtgctctc cagaacgctt atgaaggtcg gcggattaca gtaacattcg agtatgggac
     7021 cctctttcgt gacgacctcc atggtaaagc tcccttccgg aaatcccgcg cagataagcg
     7081 aaatttttct cacgcccgcc acctttaccg tcgtttttac cgtcttgtcg tgataaattt
     7141 cgtcagccga catcgggtaa tccatgtaga gctcgcatat ctggtcgagt gatactgttt
     7201 tcaccgagcc gccgtcatcc gcgaatgaga tacccgcgca tgcggctaag gcacatatcg
     7261 tcagcagaag agcccgcgcc cgggcccgat atccgtttct catcatgctc cctcgtataa
     7321 tgtctattta ttcccctgcg ccacggataa ttataacacc cgcttgtaat attttaataa
     7381 tgcgcccggt attcacggga aattttgcaa acaatcggcg gacgcatagc ttacttgata
     7441 tggaacggcc ttgaggtata gtgtcaattc aatgaagaaa aacacctgtc tcattatcga
     7501 caagagcgag gataacgccg atctttatta caggaccggc ttttttgttc ccgaccctgt
     7561 tatctacgtc gagcataacg ggaagaagac gctcgtcctg agcgacctcg agatagacag
     7621 gggcaggaag gaagccacgg tcgaccgcgt actctctctg acggaccttc ataagaggct
     7681 cgccgcggat aaggtaaaaa agataaagct tctcgacatc gccgagctcg ttctcaggga
     7741 catgaagata aagaaggtga ccgtgcccgg acggttctcg ataaaatatg ccgacggtct
     7801 caggaagagg ggttacagag tgatcccgag agacgaagag ccctttttcg agggcaggct
     7861 caggaagacg ccccgcgaaa tcggaatgat aagggattcc atgagagaga ccgtaagggc
     7921 catggactac gcaataaaaa taatcgcttc gtccgagatc agaaagaaaa gactctatta
     7981 caaaggcgct ctcctcacgt ccgaaagggt gaaggcagag ataaacgcga tgctgtccgg
     8041 caagggtttt acggcttccc atacgatcgt cgcgtgcgga gctcattcgt ccatgccgca
     8101 tcacagcggc gaggggccta tctatgcgga caagcccgtc gtcatcgaca tattccccag
     8161 gtctcagtcg agcggttact tcggcgatat gacgaggacg gtcgttcgcg gcgagccctc
     8221 acccgcgctc gtgaagatgt acgataccgt gctcaggggg cagaagctcg cgataggaat
     8281 gataagggcg ggagttaagg tcagggacat tcactcagcc gtcgtggctt atttcaaaag
     8341 ccggggtttc gagacaggga tgctcgacgg gaagatgcag ggcttcatac attcgaccgg
     8401 ccacggcctt gggctcgaga tacacgagcc gccgcgtata agcctcggcg acgaagtgct
     8461 agaggagggg aacgtcgtaa cggtcgagcc cggcctttat tacgaaaaaa ccggcggcgt
     8521 caggatcgag gacgtcgttg tcgtcgggaa gggaggctcc gccaacctca caaggtatcc
     8581 gaagaagctc atagcgaagg cttgatttac agtcgttcta tcgaattcac agtcaatccc
     8641 accacacttc gaacctgcaa atcttatcgt tcttataccg gcacgctgtc tgaattattt
     8701 ttaccgaatt cgctccggcc atcgtcagtc ccgttttgaa ccattccgga atagcgaaat
     8761 tgaatacggc gactccggac gctttttctt catcggctat ttcgtctaag tagagcaccg
     8821 agtatttttt ctccatgtcc agaagctcga gctcaagcat gccgctgtcg aaatacctgc
     8881 cccataaaga aggcgcgctc ttaagaatga cggcgggttt tttgagattc acgagagcat
     8941 cgagcatcgg gcccaggtct ttttgggcta tgtattcggc gataggtacg atcgataaga
     9001 aatccccttt tccgtgcagg acgtcgtacg tattgagcag gcggttcaaa agaggggccg
     9061 gataccaatc cgtgcttacg agatcacctt ccaatattgc ggaatccggt tccggcaaat
     9121 tatttacgat ctttttccat gcgctctcgc cgtgcgcgga tatgatatat tctttcgcat
     9181 tggagaggat cgagcctttt gttttgcctg aattatctgc cattgtcgta attcgataat
     9241 tctaaataat aatcgttatt tgtgatccat gtgttattta attttaatgt taataccgct
     9301 gatgaacatg aatacttcca attatgtacg aaaaaggaga aattatgaag aagtctttcg
     9361 atgacctggt taaattgtct atacacctga gaagtcccgg gggctgtccc tgggacaggg
     9421 agcagactct cgacacattg aaatcctata tcatcgaaga ggcatacgag gtggttcagg
     9481 cgatcgaatc acgcgacacg gacgagctca tcgaggagtt aggcgacctc ttttatgagg
     9541 tgctgttcgc ctcccagatc gctgaggaga gcctggggtt cgggatagac gacgttatcg
     9601 acaggctcca taacaagctc gtgaggaggc accctcacgt cttcggggag gagaaggcgg
     9661 caaacgccga ggaggccgtc aagaggtggc attccgagaa gctcaaggaa aagtcgagga
     9721 agcggaggct cctccacatc ccccgcacga tgccgtcgct cctgagggcg cagagggtgg
     9781 gcgagaaggc gtctcaggtg ggtttcgatt ggagcggagc ggaagaggtg gtctccaagg
     9841 tgagggagga gctctccgag ctcgaagaag agataaagag caggaacgcc tccgggatcg
     9901 agaaggaatg gggcgacctc gttttttccc tggtaaatct cgggaggcat ctgaacctcg
     9961 atgccgaggg ggccgctcac aaggccatag acagcttcat cgagcgtttc ggaaggatag
    10021 aggacaaagc ggaatcccgc ggcaaggagc tctccgaatt atcgcttaca gaaatggacg
    10081 agctttggga agaggtgaaa attgagcaca aatagtagag tgattgatgt tgcttcaatc
    10141 cggataggcg gattttaaat ctccctcccg acaaacattc cgagcagcct ccatccctcc
    10201 ggtatgaaaa cgggagaagt ggccgctgac ctttccgtat agacgaacga gtcgttcttt
    10261 atgctcacgt cctccgagct gtatatggcg aacggcacag gctcgttcac atgcgtcctt
    10321 agatcgatag gcgtcggatg gtccgacatg acgagtatcc tgtaatccct gaacctcttt
    10381 atcccttcga gcacgcgccc gacgacgcgc ccgtcgaagt cttctatcgc ctgtatcttg
    10441 agctccgctt ttccgacgtg tcccgtctcg tccgtggact cgatgtgtat catcgtcaga
    10501 tcgacttcgt taagcgaatc gagcgcgtaa tcgaccttgc cttcgtagtt ggtgtcgaga
    10561 tacccggtcg cccccgggac gtctatcact tcgagcttcg cgtagtggcc gatgcctttc
    10621 acgaggtcaa ccgccgaaat aacagagccc gtcagtccgt agagctccct gaacgacggc
    10681 attgcgggag cgactccctg tccccacagc caaatgctcg tggccgggtt cttcccctcc
    10741 gcgactctct tcttgttcac cgggtgatcc tttagtatct ccctcgaccg ctctatgagt
    10801 tcgttcagct tttcggtccc gtctcctgac ggtatgtagg cgtctatccc cttgcccgat
    10861 atatcgtgcg gcggggtagt gtgtatgtcc atattgccgc ccgcccatac gaggaggtgt
    10921 ctgtagctca cgccggggaa aaaagtcagg ttcttttcgt cgagctcttt tttcaggtcg
    10981 aggattatat tgcgcgcctc ttccgttgtt atatgccccg cgctgtaatc atccattacg
    11041 aggcccccct gtccagagac gagggtcacg aggttgcacc tgagcgtgac gtctccgtcc
    11101 ctgagcggaa cgcctatgct cgcggcctcg aggggagacc tgcccgtgta atagaggtgg
    11161 gggttatatc cgagcactgt caggttgcct acgtcgcttc cgggcggaag gtcgtcgggt
    11221 atggtcttca ccatgccgaa cttgatcgcc gacttggcta tcctgtcgag gttgggagtc
    11281 ctcgcggcct ctagcggcgt ctttccgccg agctccggca gagggtggtc gggcatcccg
    11341 tcgccctgca gtatcacgta tttcatgagc tatgatgtta aatgcttttc gggatttatc
    11401 aagtataaaa gattggttag acaggccaaa cggcagtttg ttcggattat aggaggtata
    11461 tcaggatagt gcgtgcccgg acatcttcac cacctgcttc tcagcgtggt acgagctcct
    11521 gacgagcggg cctgattcga cgtgcggtat ccctatcacg ttctcgccgt aggttttaag
    11581 cgattcgaac tccatcggat ggtagaactt cgatacggag aggtggtcgt aagtcggctg
    11641 gaggtactgt cctatcgtta ctatatcgca cccggccttc ttcaagtccc cgagcgtctc
    11701 tatgacttcg tccatctcct ctccgagacc gaccattatg cccgatttcg tgagcatgcc
    11761 ttcgtgaccg agcgttttgc tctcggaaag cagtctgagc gaccagtcgt aatttgcctg
    11821 gggcctcacc gaccttcttt tccccgaagg cgtcatctgc ggcagacggt agagcctagg
    11881 cacggtttcg atattgtggt tgagcacgtc gggccgtgcg tttaaaactt ccccgagagc
    11941 gccccgttcc cccttgaaat cgggaatgag cacctctatc ttcacttcag ggttctcagc
    12001 cctcaccttc cttatggtct ctgcgaagat gcacgcgctt tcgtagttcc tgtcgtcacg
    12061 gtttactgac gtgattacta tatgcgtgat acgggcgtta ttacgagtga ggtccctcac
    12121 tgcctcggcc actctcgcgg gctcctcata gtcgagctct ccccccctcc cggttttcac
    12181 gtggcagaag ccgcagctcc tcgtgcacgt gtctcccagt atcatgaacg tgagcgtgcc
    12241 agcgccccag cactccccga tgttgggaca cctggcctct tcgcacaccg tatgcaggtt
    12301 gagagtcctc atcaggttct tcagatgagt gtaattgggg cctctcggga gctgggcctt
    12361 tatccagtcg ggt
//
LOCUS       DASSJH010000364         9005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_196588, whole genome shotgun sequence.
ACCESSION   DASSJH010000364 DASSJH010000000
VERSION     DASSJH010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196588"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1051)
                     /gene="purM"
                     /locus_tag="VFJ67_08055"
     CDS             complement(11..1051)
                     /gene="purM"
                     /locus_tag="VFJ67_08055"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET7290373.1"
                     /translation="MAKTTYKGSGVNIDAGNLFVELIKPLAAATLNKHVLGRIGGFSG
                     AYELPLKQYKKPVLVAATDGVGTKLKLAFMTGRLDTVGIDLVAMNVNDLIACGADPLF
                     FLDYIATSKLDPAEGAEIIKGIVEGCREAGCALLGGETAEMPGFYGKGEFDLAGFAVG
                     IVDKDEIIDGSDVLPGDAVIGLASSGLHSNGYSLARKVLLDMKKYGLGESPGPLPRTL
                     AEELLEPTRIYVKTVRKLRQKFRINAIAHITGGGLLENIPRVLPKKYAALLESASWNL
                     PPVMELIRRDGHIDADEMRRTFNCGIGLVIVVKSSDADRVLKRIRGLGEMAYLIGEIV
                     KRGKGGHGVTIK"
     gene            1234..2121
                     /gene="glyQ"
                     /locus_tag="VFJ67_08060"
     CDS             1234..2121
                     /gene="glyQ"
                     /locus_tag="VFJ67_08060"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET7290374.1"
                     /translation="MTFQELILSLQSYWAKKGCIIMQPYDLEKGAGTFHPATFLRCLG
                     PEPWYVAYVEPSRRPTDGRYGDNPNRLQHYYQFQVIIKPSPANIQDLYLDSLRHLGID
                     PLAHDIRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQQAGGIDLKPVSAELTYG
                     TERIAMYLQGVESVYDLEWTKGITYGEIHHRDEFEFSTYNFQESDPVMLRDLFDKFEA
                     ECGKLIRKKLPLPAYDYCLKCSHTFNLLDARGAIGVTERASYIGRVRALAKMCAEGYL
                     LEREEMGFPLAKKNPWKKS"
     gene            2106..4193
                     /gene="glyS"
                     /locus_tag="VFJ67_08065"
     CDS             2106..4193
                     /gene="glyS"
                     /locus_tag="VFJ67_08065"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET7290375.1"
                     /translation="MEKELILEIGTEEIPAGFMNEAIRSLSEITERKLRENAISYGGI
                     SSFGTPRRLALRVTGLGDEQEDRSVESYGPPVRIAFDEKGNPTNATIGFAKSQGIRVE
                     ELVTVKRDRGEFLAAVKKIKGQKTEKLLKEILPGIIGELRFRKSMKWGDGDYVFARPI
                     RWILAVYGGKPVGFVIGDVKSSSKSFGHRFMSPKAFNVSFWEDYSTGLEKGCVMLDQE
                     KRKRYIEKEISNLAAKIGGTAHIDEELLDTVTYLVEYPVVLKGDFEESFLDLPKEVLI
                     SVMKNHQKYFPVESGDGDGGLLPHFIFVAGTKAGDDSVVIKGNERVIRARFTDARFFY
                     NEDRSAPLAEKAGRLKGMVFLSDLGTYYDKTERIKLVAGHIGKELGFESMLPDIVRAA
                     GLSKADLATQMVFEFPELQGIMGRYYALLSGEKNDVATAIEEQYKPTSREGDLPGSAI
                     GSVISIADKTDTISSCFASGLVPTGTSDPYALRRQAIAIINIALEKNYHLSIGALFRA
                     ALGAIISQTNGKFRERQDNIFAEITAFIKERFRNIMISDGFPQDVVEAAVSVEFDDVV
                     EVKRRIEALAEFRKAPDFESLGVAFKRVVNIVKGQPRGEVRESVFVEPAEKDLYMSFV
                     ETREKAGAKMSEKNYRESLSIMRKLKEPVDKFFDSVLVMDENEELRNNRLSMLWEIRE
                     LFFRIADFSKLST"
     gene            4250..6973
                     /gene="ppdK"
                     /locus_tag="VFJ67_08070"
     CDS             4250..6973
                     /gene="ppdK"
                     /locus_tag="VFJ67_08070"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET7290376.1"
                     /translation="MKKFVYLFGNGKAEGKGDMKDLLGGKGAGLAEMTRIGVPVPPGF
                     TISTEACAEFYVHKRHLPEEILKEIEQNISKLEKLIGKKLGDPENPLLVSVRSGAKFS
                     MPGMMDTILNLGLNDKIAERLSSKTDNPTFVWDAYRRLVEMFSDVVFGIDKEEFEHLF
                     AGIKKKHGVKNDSQLTPEILKEVIKQSKQHVKKKVKAEFPQSPLDQLHMAIRAVFLSW
                     NNERAIFYRKQYSIPDDLGTAANVQAMVFGNMSDDSGTGVGFTRDPASGEKQLYAECL
                     MNAQGEDLVAGIRTPKHIEELAKELPQAYKQLMKIADSLEKHFKDMQDFEFTIEKGKL
                     YFLQTRNGKRTGIAALKIAYDMVREKLITREEAISRIEPEHLEQFLFPIFNPEEKEKH
                     KIIATGLAASPGAVSGAVALDADTAVRFSSEGKNTILVRKETSADDIKGMAASKGVLT
                     ARGGRTSHAAVVGRQMGKVCIVGAEDINIDEKNRRFVTGGVTVKEGDFISLDGFEGKV
                     YSGHVPVIPSDIIQVVEERIKPEQSENYKIFTAILKWADEIRTIGVRTNADTPQDAKI
                     AYKFGAEGIGLCRTEHMFFAKDRIHIMQDMILSQTPEERASYLAKLLPMQRNDFKELF
                     RNMTGHPVTIRLIDPPLHEFLPKLEELMLEIKELELKNKSSKILDRKKLLLERVQELH
                     EFNPMLGLRGCRLGILIPEITRMQVTAIMEAATEVSEEGVKAVPEIMVPLVAMVTEMK
                     AQKDVIQEVAEEVLRKSKRKVKYKIGTMIEVPRAAVTADQIATEAEFFSFGTNDLTQM
                     MFGLSRDDSGKIIKTYMNEKVTLNGKTSSILEKDPFLTLDLGVVELMKRAIEKGRRIR
                     PDLKVGVCGEHGGDPQSIVYANSIGVDYVSCSPFRVPIARLAAAKGALEKK"
     gene            complement(7090..8652)
                     /gene="rny"
                     /locus_tag="VFJ67_08075"
     CDS             complement(7090..8652)
                     /gene="rny"
                     /locus_tag="VFJ67_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET7290377.1"
                     /translation="MTELMVAVAAAVVAFALGYLANHLYRQMKLNKKISLKKDEAEVI
                     IDNARKEADKIRVQGELKAKEIVERKSAEIENESREKQREAFKTEKRLQKREEVLDKK
                     FEAVEKRDSELSRKERDVIQREQALGEKQKELDAVIDEAKKKIEDIAGMTQEAARNEL
                     INIIEDEARHEAAKKLKQLEDQLQEEAERKAKDVIALAIQKYAGEYTSEKTISVVNLP
                     NDDMKGRIIGREGRNIRSIEARTGVDIIIDDTPETVVISSLNPIRREVARISLEKLID
                     DGRIHPARIEEIVSDVEKQIEREIQKAGEAAIFDLGIPSIHPELVKLVGRLKYRTSYS
                     QNILNHSIEVAFICGILAAEIGYDVKLAKRGGLLHDIGKAVDHEIEGSHVDIGEDLVR
                     KYGEPKDVIEAVALAHDPQPQSLLAILVQSADAISAARPGARRETYEAYVKRIEDIES
                     IAGSFRGVEKCYAIQAGREVRVIVESSKVNDEEGALLSHEIAKKIEKEVAYPGQIKIT
                     VIREVRSTAFAS"
     gene            complement(8645..>9005)
                     /locus_tag="VFJ67_08080"
     CDS             complement(8645..>9005)
                     /locus_tag="VFJ67_08080"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET7290378.1"
                     /translation="GLTFHRILDLNELKPGKFGIPEPDSSSSSIAPEDLDLILIPGIA
                     FDGSGVRLGYGKGYYDRLLVNVPLRRRAALAYSLQMSDSLPCGETDLSAGLVVTESGI
                     IYRGMKGRIKEGGKQHD"
BASE COUNT         2187 a         2409 c         2499 g         1910 t
ORIGIN      
        1 ccactgctcg tcattttata gtcaccccgt gaccgccctt cccccgtttc actatctctc
       61 cgataagata ggccatctct ccgagcccgc ggattctttt gagcacacga tccgcatccg
      121 aggactttac aacgatcacg agtcctatac cgcagttgaa cgtcctccgc atctcatccg
      181 cgtcgatgtg tccgtccctc ctgatgagct ccatgacagg aggcaggttc catgaagcgg
      241 attcgagcag ggcggcatac tttttcggca atactctcgg tatgttttct aaaagcccgc
      301 cgcccgtgat gtgggcgatc gcgtttatgc ggaacttctg tctcagcttc cggacggttt
      361 ttacgtagat ccgggtcggc tcgaggagct cctcggcgag agtgcgcggc aggggcccgg
      421 gagattctcc gagtccgtat ttcttcatat ccaggagcac ttttctcgca agtgaatacc
      481 cgttcgaatg gagaccgctt gaagcgagcc caatgacggc gtcacccgga agcacgtccg
      541 agccgtcgat gatctcgtcc ttgtccacta tgccgactgc gaagcccgcg agatcgaact
      601 cgcctttccc gtagaagccg ggcatctcgg ccgtttcccc gccaaggagg gcgcacccgg
      661 cctccctgca gccctctacg attcccttaa taatctccgc cccttcggcg ggatcgagct
      721 tcgaggtggc tatataatcg aggaagaaga gagggtcggc gccgcaggcg atcaagtcgt
      781 tcacgttcat ggccacgagg tcaataccga ccgtgtcgag cctgcctgtc atgaaggcga
      841 gcttcagctt ggtccccacg ccgtccgtcg cggctacaag cacgggcttc ttatactgct
      901 tgagcggaag ctcgtaagcg cccgaaaagc cgccaatcct gccgaggacg tgtttattca
      961 gcgtggccgc ggccaggggt tttataagct cgacaaagag gttaccggcg tctatattga
     1021 cgcccgagcc cttgtaagtg gtctttgcca tgacgaaagg gaaagatacc tgagagaggg
     1081 ggtttttgtc tacgaaccgc ccttaacgaa gcaccctttt gatgaccttt cggaaaagcc
     1141 gggcggtcct gacggggtgc agccccgaaa tttccaaaaa attcaaataa tggtatattt
     1201 ttccctctga ttttgcgggc tgaagggaaa taaatgactt ttcaggaact gatactatca
     1261 ctccagagtt actgggctaa aaagggctgt attatcatgc agccttacga cctcgagaaa
     1321 ggggctggaa cgttccaccc ggcgaccttt ctccggtgtc tcgggcccga gccctggtac
     1381 gtggcctatg tcgagccttc gaggaggcct accgacggca ggtacgggga caacccgaac
     1441 aggcttcagc attactacca gttccaggtg ataatcaagc cctcccccgc gaacatacag
     1501 gatttatatc tcgacagcct gaggcatctg ggtatagacc ctctcgctca cgatatcaga
     1561 tttgttgagg acgactggga atccccgacc ctcggcgctt ggggcctcgg atgggaggtg
     1621 tggctcgacg ggatggagat cactcagttc acgtattttc agcaggcggg aggaatagac
     1681 ctgaaacccg tctccgcgga gctgacgtac ggcactgaaa gaatagcgat gtacctgcag
     1741 ggcgtggaaa gcgtatacga cctcgagtgg acgaaaggca taacttacgg cgagatacac
     1801 cacagggacg aattcgaatt ctcgacgtat aactttcagg aatcggaccc ggtcatgctg
     1861 agggacctgt tcgacaagtt cgaggcggag tgcgggaagc tgatccgaaa aaagctcccc
     1921 ctccccgcgt acgactattg cctgaaatgc tcccacacgt tcaacctcct tgacgcccgg
     1981 ggggcaatag gggtcacgga gcgggcttca tatatcggga gggtcagggc gctcgcgaaa
     2041 atgtgcgctg aggggtatct cctcgaaaga gaggaaatgg gtttccccct cgcaaagaag
     2101 aatccatgga aaaagagctg atacttgaaa tagggacgga agagatcccg gccggcttca
     2161 tgaacgaagc tatccggagc ctttcggaaa taacggagcg caaactgagg gagaacgcca
     2221 tctcttacgg cgggatatcg tccttcggca cgccgaggag gcttgcgctc agggtcaccg
     2281 gcctcggcga cgagcaggaa gaccgttccg tagagtcata cggccccccc gtaaggatag
     2341 ccttcgacga aaaaggcaat cccacaaacg cgactatcgg tttcgcgaag tcgcagggga
     2401 taagagtgga agagctcgtt acagtgaaaa gggacagggg cgagttcctg gccgccgtaa
     2461 aaaagatcaa gggtcagaaa acggaaaagc tcctcaagga aatactgccc gggatcatag
     2521 gcgagctccg tttcaggaag tcgatgaaat ggggtgacgg cgattacgta ttcgcgagac
     2581 ccatcagatg gatactggca gtctacggcg gaaagccggt cggtttcgta atcggagacg
     2641 taaagagctc gtccaaatcc ttcggacaca ggttcatgag cccgaaggcg tttaatgtct
     2701 cgttctggga ggattattcc acgggcctcg aaaaaggctg cgtaatgctc gatcaggaaa
     2761 agaggaaaag atacatcgaa aaagagattt cgaaccttgc cgcgaagatc ggcgggacgg
     2821 cgcacataga cgaggagctg ctcgatacgg tgacgtatct agttgagtat cccgtcgtgc
     2881 tgaagggcga tttcgaggag agcttcctcg acctcccgaa agaggtcctc ataagcgtga
     2941 tgaaaaacca ccagaaatat tttcccgtag agtccggaga cggagacggc gggctcctcc
     3001 cgcatttcat attcgttgca ggcacgaagg ccggggacga cagtgtcgta attaaaggaa
     3061 acgaaagggt aataagggcg aggttcacgg acgcgaggtt cttttacaac gaggacagga
     3121 gcgcgccatt ggccgagaag gccgggcggc tgaaggggat ggtctttctt tccgacctcg
     3181 gcacttacta cgacaagact gagagaatca agctggtcgc agggcatatc ggtaaagagc
     3241 tcggtttcga aagcatgctt ccggatatcg ttcgtgcggc cgggctgtcc aaggccgacc
     3301 tcgcgacgca gatggtgttc gagttcccgg agctccaggg cattatgggc agatactacg
     3361 cgctcctctc gggcgagaag aacgatgtcg cgaccgcgat agaggagcag tacaagccca
     3421 catcgcggga aggggatctg cccggatcgg cgatcggctc cgtcataagt attgccgata
     3481 aaacggacac catatcctca tgcttcgcct cggggctcgt ccctacaggc acttcagacc
     3541 cgtacgcgct caggagacag gcgatcgcaa taataaacat agccctcgaa aaaaactacc
     3601 atttgagcat cggcgcgcta ttccgggcgg cgctcggcgc gataatctcc caaaccaatg
     3661 gaaagttccg agagcgtcag gataatatat tcgcggaaat cacggccttt attaaagaga
     3721 ggttcagaaa tataatgata tcggacggct tccctcagga cgttgtggag gccgcggtaa
     3781 gcgtcgagtt cgacgacgtg gtcgaggtga agaggagaat cgaagcgctt gccgagttca
     3841 ggaaggcgcc tgatttcgaa tccctcggag tagcgttcaa gagagtggtg aatatagtta
     3901 aaggacagcc caggggcgag gtcagggaga gcgtttttgt ggagcccgcg gagaaggatc
     3961 tctatatgtc attcgtcgag acgcgtgaaa aggccggggc gaaaatgtcg gagaagaatt
     4021 accgggagtc cctttccata atgagaaagc ttaaagagcc ggtggataag ttcttcgaca
     4081 gcgtgctcgt aatggacgag aacgaagagt taaggaataa caggctctcg atgctctggg
     4141 aaataaggga gctgtttttc aggatcgcgg atttttccaa attaagcaca taagggtcaa
     4201 tgtaaaacag gcgttggatt aaaaaagaat acatacaaag gacgaagaca tgaagaaatt
     4261 cgtatatctc ttcggcaacg gaaaagccga aggaaaaggc gacatgaagg acctgctcgg
     4321 cggcaagggg gccggtctcg ccgagatgac aaggataggc gtacctgtcc cccccggctt
     4381 tacgatcagc acggaggcct gtgcggaatt ctatgtgcat aaaagacacc tcccggaaga
     4441 aatacttaag gaaatcgagc aaaatatctc gaagctcgag aagctcatag gcaaaaaatt
     4501 gggcgacccc gagaatccgc tcctcgtttc cgtccgctcc ggggcgaagt tctccatgcc
     4561 cggaatgatg gatacgatac tcaacctcgg cctcaacgat aagattgccg agcgtctctc
     4621 gtcgaagacc gataacccga cgttcgtgtg ggacgcttac agaaggctcg tcgagatgtt
     4681 ttccgacgtc gtgttcggca tagacaagga agagttcgag cacctgttcg caggtataaa
     4741 gaagaagcac ggtgtcaaaa acgattccca gctgacgccc gaaatcctga aagaggtcat
     4801 aaaacagtcg aagcagcacg tcaaaaagaa agttaaggcc gaattccccc agagcccgct
     4861 cgaccagctc cacatggcca tacgcgccgt attcctttcc tggaacaacg agcgggccat
     4921 cttctacagg aaacagtaca gtatccccga tgacctcggg actgcggcaa acgttcaggc
     4981 catggtcttc ggcaatatga gcgacgattc gggcacgggt gtgggtttca cgagagaccc
     5041 cgcaagcgga gagaagcagc tctatgccga atgtctcatg aacgcccagg gggaagacct
     5101 cgtggccggc atcaggacac ccaagcatat cgaggagtta gctaaggagc tgccgcaggc
     5161 atataaacaa ctcatgaaga tcgcggattc cctcgaaaag catttcaagg acatgcagga
     5221 cttcgaattc acgatcgaga agggaaaact atatttcctt cagacacgaa acgggaagag
     5281 gaccggtata gcagcgctaa agatcgcata cgacatggtc agggaaaagc tgatcacgag
     5341 ggaggaggcg atctcgagga tcgagcccga gcacctggag cagttcctgt tcccgatttt
     5401 caatccggaa gaaaaagaga agcataagat catagccaca gggctcgcgg cgtcaccggg
     5461 agcggtgtcg ggagccgtgg ccctcgacgc ggacacggcc gtaaggttca gcagcgaggg
     5521 gaagaacaca atactcgtca ggaaagaaac gagcgccgac gacatcaagg ggatggccgc
     5581 gtcgaaggga gtgctgaccg cgaggggagg aaggacgagc cacgcggccg tggtgggaag
     5641 gcagatggga aaggtatgca tcgtgggggc cgaggacata aacatcgacg aaaagaacag
     5701 gcggttcgtg acgggagggg ttacggtaaa ggaaggcgat tttatttcgc tcgacgggtt
     5761 cgagggaaag gtgtattcgg gccacgttcc cgtcattccg tcggacataa tacaggtcgt
     5821 cgaggaaagg ataaagcccg agcaatcgga gaactacaag atattcacgg cgatactgaa
     5881 atgggcggac gagatcagga ccataggggt gagaacgaac gccgatacgc ctcaggacgc
     5941 gaagatagcg tacaagttcg gcgccgaagg cataggactc tgcaggaccg agcacatgtt
     6001 cttcgcgaaa gacaggatcc acataatgca ggacatgata ctctcgcaga cacccgaaga
     6061 gagggcgagc tacttggcga agctgctgcc gatgcagagg aacgacttca aagagctttt
     6121 ccgcaacatg acggggcacc cggtcacgat aaggcttatc gacccgccgc tccacgagtt
     6181 cctgcccaag ctcgaagagc ttatgctcga aatcaaggag ctcgagctga agaacaagtc
     6241 ttcaaaaata ctcgacagga aaaaattact tctggagaga gtccaggagc ttcacgaatt
     6301 caaccccatg ctcggtctca ggggctgcag gttgggcatc ctcattccgg agatcaccag
     6361 gatgcaggtg acagcgataa tggaagcggc gacggaggtg tctgaggaag gggtgaaggc
     6421 ggtccctgaa ataatggtcc cgctcgtggc tatggttacc gaaatgaagg cgcagaaaga
     6481 cgtaatccag gaagtagccg aagaagtcct cagaaagtcc aaaaggaaag tcaaatacaa
     6541 gattggaacg atgatagaag tcccgagggc ggccgtaacg gccgaccaga tagcgaccga
     6601 ggccgagttc ttttcattcg gcacgaacga cctgacgcag atgatgttcg gactatcgag
     6661 ggacgactcg gggaagatca taaagacgta catgaacgaa aaagtgacgc tcaacggaaa
     6721 gacttcgagc atcctcgaaa aagacccttt cctgacgctc gacctgggcg tcgtcgagct
     6781 catgaagcgg gcgatcgaga aagggaggag gataagaccc gatctcaagg tgggtgtatg
     6841 cggagagcac ggcggggacc cgcagtcgat agtctatgcg aactcgatag gggtcgatta
     6901 cgtgagctgc tcccctttca gggtgccgat cgcgaggctc gccgcagcca aaggcgcgct
     6961 cgagaagaaa tagaattgaa aataacgaat aaaaaagcct gagccggcaa tttacaaacc
     7021 ggctcaggct tttctcgtcc ttattaattt aatatctcgt caattccaaa tacagatcag
     7081 caaccggaac tagctcgcaa acgccgtaga ccttacttct cttatcacgg ttatctttat
     7141 ctgtccgggg tatgcgacct ctttctcgat cttcttcgct atttcgtgcg aaagcagagc
     7201 gccctcctcg tcgttcacct tcgagctctc gacgatgacg cgcacttcgc gtcccgcctg
     7261 gatcgcataa cacttctcga cccctctgaa cgagcccgcg atgctctcga tgtcttctat
     7321 gcgtttgacg taagcctcgt acgtctctct cctggccccc ggcctcgcgg ccgatatggc
     7381 gtcagccgac tgcaccagga tcgccagaag cgactggggc tgcggatcgt gcgcgagcgc
     7441 cactgcctct atcacgtcct tgggctctcc gtacttcctc acgaggtcct cgcctatgtc
     7501 cacgtgcgaa ccctctatct cgtggtcgac cgcctttcct atgtcgtgca gcagcccgcc
     7561 gcgcttggcg agcttgacgt cgtacccgat ctccgcggcc agaatgccgc agatgaatgc
     7621 gacctcgatc gaatggttga ggatattctg cgagtagctc gtcctgtatt tgagccttcc
     7681 gacgagcttc acgagctcgg ggtgtatgga cgggataccc aggtcgaata tcgccgcctc
     7741 tcccgccttc tgaatctcgc gctcaatctg cttttcgaca tcggacacta tctcctctat
     7801 cctggctggg tgtatcctgc cgtcgtctat cagcttctcg agagatatcc tcgcgacctc
     7861 cctccttata ggattgagtg aggagataac cacggtctcc ggcgtatcgt cgatgatgat
     7921 atcaacgcct gtccgcgctt ctatcgacct tatattcctt ccctcgcgtc cgatgatcct
     7981 gcccttcatg tcgtcgtttg gcaggttgac cacggatatg gtcttttcac tcgtatattc
     8041 gcccgcatat ttctgaatag cgagcgctat tacgtccttc gccttcctct cggcctcctc
     8101 ctggagctgg tcctcgagct gcttgagctt cttggcggcc tcatgtctcg cctcgtcctc
     8161 gattatattt atcagctcgt tccttgccgc ctcctgcgtc atacccgcga tatcctctat
     8221 ctttttcttc gcctcgtcta taacggcgtc gagctccttc tgtttttctc cgagggcctg
     8281 ctccctctgt attacgtccc tttccttcct tgaaagctcc gaatccctct tctcaacagc
     8341 ctcgaatttc ttatcgagca cctcttcgcg cttctgaagc cttttttccg tcttgaaggc
     8401 ctctctctgc ttctccctcg actcgttctc tatctcggcg ctctttcttt cgactatctc
     8461 ttttgctttc agctcaccct gcaccctgat cttatcagct tctttcctgg cattatcgat
     8521 aataacctct gcttcgtcct tctttagact tatcttctta ttcaatttca tttgtctata
     8581 gaggtggtta gctaagtacc ccaaagcgaa ggcaacaacc gcagccgcta cagcaaccat
     8641 cagttcagtc atgttgtttt cctccttctt ttattctgcc tttcattccg cgataaatta
     8701 ttcccgattc ggtcaccacc agacccgcgg aaaggtcggt ctccccgcat gggagcgaat
     8761 ccgacatctg gagcgagtat gcaagcgcgg cccttctgcg gagggggaca ttaaccagca
     8821 ggcggtcata ataccccttc ccatacccga gcctcacgcc ggagccgtcg aatgcgattc
     8881 cgggaataag gatcaggtcc aggtcttcag gcgctatgct tgatgaggac gaatctggtt
     8941 ccgggatacc gaatttcccg ggcttaagct cgttcaggtc taatattcta tggaaagtca
     9001 gtccg
//
LOCUS       DASSJH010000365         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1494789, whole genome shotgun sequence.
ACCESSION   DASSJH010000365 DASSJH010000000
VERSION     DASSJH010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494789"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFJ67_08085"
     CDS             <1..363
                     /locus_tag="VFJ67_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290379.1"
                     /translation="MEEPGGFYCTIYVNENEIYSGELGEVPEEFKTRMARDLGEWADS
                     LGKRGLNELIYSHLAWYEEKAVYCGECGKRYDDGSAGVCGECGGKLIERYVYDRDKGL
                     DMIITCVGMITKVQVSKI"
     gene            426..731
                     /locus_tag="VFJ67_08090"
     CDS             426..731
                     /locus_tag="VFJ67_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290380.1"
                     /translation="MKLTVKGQIFPKVWDIAESVRKLRDDRKPVNIGAGTVGFSVYEV
                     ELPEDTRKEGLYFVTPQGIKLYFYPGDQYQIAYLISEIDSDPLKEKHFKETAGAVPS"
     gene            792..1721
                     /locus_tag="VFJ67_08095"
     CDS             792..1721
                     /locus_tag="VFJ67_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009335631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7290381.1"
                     /translation="MEHHEHSHDIKNTKEKKALTLVLAFTACFMVVEAVSGFYTGSLA
                     LLSDAAHMLTDVFALSLAYFALWFASKPPTMSKTFGFYRGEILAAFLNGILLFVISGW
                     IIFEAYERFRVPHPVKSLDMTIVAVVGLVLNLGSAYILSGYQTSNLNIKGALYHVISD
                     ALGSAGAVAAGVVMILTDWYYADSIISVLVSVLILRSAWLIIKESVHILLEGTPRGID
                     LKAVQSSICSHEGVLGVHDLHAWTLTQGFEALSAHLVVEDMGESERLIGEIKNNLSGK
                     FHITHVTLQCETKECASAFNTCYEDAAGGKLSG"
     gene            complement(1722..2135)
                     /locus_tag="VFJ67_08100"
     CDS             complement(1722..2135)
                     /locus_tag="VFJ67_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290382.1"
                     /translation="METEAGWECKYQFRETPARRFITVELGEIGSEGQEMSITDRFSP
                     ADTEGAVVRVRYEVGSPGAEVDEKEIRSAFGGAQTLKIERVFSKPGKTMRQSGLSKTM
                     SVLEALDKYIQSKPELKKIGDEMKARAEKLIRESE"
BASE COUNT          484 a          609 c          596 g          508 t
ORIGIN      
        1 atggaagagc ctggcggttt ttactgcacg atatacgtga acgaaaacga gatatattcc
       61 ggggagctcg gtgaagtccc cgaagagttc aagacgcgca tggcgcgcga tctcggcgaa
      121 tgggcggact cgctcggtaa gaggggcctg aacgagctga tatactccca cctcgcatgg
      181 tatgaggaaa aggcagtgta ttgcggggag tgcgggaagc gttatgacga cgggagcgcg
      241 ggagtgtgcg gggagtgcgg gggcaagctc atcgagcgct acgtctatga tcgcgacaag
      301 ggtctcgata tgatcatcac ctgcgtgggt atgatcacca aggtacaggt ttcaaaaata
      361 taacgggtcc ataaaatgtc ccgcccgtgt ttcgcatggc ggggttgaga cacgggaggt
      421 ctcggatgaa gctaacggtc aagggtcaga tattccccaa agtgtgggat atcgcggaga
      481 gcgtcaggaa gctgagagac gacagaaaac cagtcaacat cggcgctggc accgtcggat
      541 tctccgtcta tgaagttgag cttcccgaag atacgagaaa agagggcctg tatttcgtaa
      601 cgcctcaggg cataaagctc tacttttacc ccggtgacca gtatcagata gcctatctca
      661 tctctgagat agacagcgac ccgcttaagg aaaaacattt caaagagacg gccggtgccg
      721 tcccttcctg atgtcccttg gcaaccggcg cgctttgact cccctacggt gtgtatgtat
      781 ttttaattcc gatggagcat cacgagcact ctcacgacat taaaaacact aaagaaaaaa
      841 aggcgctcac cttagtcctc gctttcaccg cgtgcttcat ggtggtggag gcagtatccg
      901 gattctacac gggcagtctc gcgctcctct cggacgctgc gcacatgctg acggacgtat
      961 tcgccctctc gctcgcctat ttcgcgctgt ggttcgcatc gaagccgccg acgatgagca
     1021 agacattcgg cttctacagg ggagagatac tcgccgcgtt cttaaacggc atcctcttat
     1081 tcgtgatctc gggctggatt atttttgaag cttacgagag gttcagagtg ccccacccgg
     1141 tgaagagcct cgacatgacc atagtagcgg tagtgggtct tgtgctcaac ctgggctcgg
     1201 cttacatact gtccggatat cagacctcga atctgaacat aaagggcgcg ctctatcatg
     1261 tcataagcga cgcgctcggc tcggctggcg ccgtcgcggc gggcgtcgtg atgatcctca
     1321 cggactggta ttacgctgac tcgatcataa gcgtcctcgt ctccgttctc atactcagga
     1381 gcgcatggct gataataaag gaatcggttc acatcctttt ggaaggcacg ccgcggggta
     1441 tcgaccttaa agcggttcag agctcgatat gctcccacga aggggtctta ggcgtgcacg
     1501 acctccacgc gtggacgctt acacaggggt tcgaggcttt aagcgcccac ttagtcgtcg
     1561 aggatatggg agagagcgag aggctgatcg gcgagatcaa aaataatctg tccgggaaat
     1621 tccacatcac acacgtaacg ctccagtgcg agacaaagga gtgcgcgtcc gctttcaaca
     1681 cctgttacga ggatgccgcg ggcggaaagt tatcaggata gtcactcgct ctcccgtata
     1741 agcttctcgg cgcgggcttt catttcgtcg ccaatctttt tcagctctgg tttggactga
     1801 atgtacttat ccagcgcctc gagcacgctc atcgtcttcg agagccccga ctgcctcatc
     1861 gttttcccag gcttggagaa gaccctctcg atcttgagcg tctgtgcgcc gccgaacgcc
     1921 gaccttatct ccttctcgtc gacctcggcc cccggactcc cgacttcata tctgaccctg
     1981 accacagcgc cctccgtatc cgcaggagag aacctgtcgg tgatagacat ctcctgtccc
     2041 tcagaaccta tctcgccgag ttcgacggtt atgaatctcc tcgccggggt ctccctgaat
     2101 tgatacttgc actcccatcc cgcctctgtt tccattatct cgcccaggac gaagccctgc
     2161 ttctcttttt cttacgtgaa atctatcctc tctatcc
//
LOCUS       DASSJH010000366         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_622620, whole genome shotgun sequence.
ACCESSION   DASSJH010000366 DASSJH010000000
VERSION     DASSJH010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622620"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFJ67_08105"
     CDS             <1..498
                     /locus_tag="VFJ67_08105"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET7290383.1"
                     /translation="GRLVKESGAESVKLEINEDYVETLYAINKAGIPVIGHIGLCPQS
                     VLEMGGYKVQGRGRHEARRLLDLAKAIESAGAFSILIESIPRTLAAEITKSVKIPTIG
                     IGAGPGCDGQVLVINDLLGLSPEPIPKFVKKYANLREVVDKSTKAFIEDVRSGAFPSE
                     EHSYD"
     gene            491..1348
                     /gene="panC"
                     /locus_tag="VFJ67_08110"
     CDS             491..1348
                     /gene="panC"
                     /locus_tag="VFJ67_08110"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET7290384.1"
                     /translation="MTERGTVKVVNGIGEMQALSNSLRIEGRIISFVPTMGALHEGHL
                     SLMREGKKHGDVLIASVFVNPTQFGRGEDFQSYTRDTEGDLKKMESAGADIAFFPRAD
                     EIYPPGYETYVEVTELEKPLCGRFRPGHFRGVATVVLKLFNIVKPHVALFGEKDYQQL
                     QIIKKMVRDLNLEVGIIGMPIIREESGLALSSRNAYLTREEREKATCLSRALGQIKKS
                     FDAGERDTARLAGEGYCMLAGASIGEVDYIEIRDGETLGEKRLASAGDVAAIAVRIGG
                     ARLIDNKVL"
     gene            1378..1767
                     /gene="panD"
                     /locus_tag="VFJ67_08115"
     CDS             1378..1767
                     /gene="panD"
                     /locus_tag="VFJ67_08115"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET7290385.1"
                     /translation="MHRTLLKSKIHRVTVTDANIEYEGSITVDRDLMDAADFHHYEQV
                     HIFNVSNGHRLVTYVIEGERGSGDICVNGAAAHLARKGDCLIVASFASYSESECKTQV
                     PRLVYVDGQNRITRIGPEQKTLKVAKS"
     gene            2069..2842
                     /locus_tag="VFJ67_08120"
     CDS             2069..2842
                     /locus_tag="VFJ67_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HET7290386.1"
                     /translation="MKAVILAAGRGKRLYPYTKYIPKCLLDIGGETILEHQINHLRDC
                     GIDEVVIVVGFGFEKVENFLRNYDGLGMRIKTLYNPFYQTTNSLISLWIARGEMDDDI
                     VVMNGDDVFEIEVLEQALRMRDEKICLPIKRKSSYEDEDMKVVIKETKIVDISKTLTN
                     RASAESVGVRVFRDTGVELMKRAIEEEMRTVGAENKWYISAIHRLIKKGYKVKSLDIN
                     DLFWMDVDYPSDLFKARFNSNKFIKKSYQERVLRVVETS"
     gene            2846..4168
                     /locus_tag="VFJ67_08125"
     CDS             2846..4168
                     /locus_tag="VFJ67_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7290387.1"
                     /translation="MDHAIIVAAGTGIEEEKLSLHGKTVFGSLPQIKRLVITAQRAGI
                     KKFTIITETGDTSLKDLLAGDRRIESDIGWHSLGSEIKLDSAHCLIIQSNLITTPRSL
                     ANFMESDCAQDEVAVLVDSLDDAWIKASGEEISDVFSWGGKAVGAFIVSGRLLEKSIM
                     NSMSLRTFVQEHIGRDKVKLRKFTDSYWMRLDSGEDAAARAEDLIFAHVGKTATGWLS
                     RNINSKISLPTSRLLVRTPLTPNMISVLINIIGMMCGVFYAIGHPVWGALCMQAATIL
                     DRCDGEVARVKLMETHRGQWVDTISDQVTVLSFMLGVPIGYYVISKSPLAVILGAVNV
                     SIFIFFVVWSFYFLKKYTNSGSLVTYFDVDKLVEGRKASLMRRLIKIVRPMGRRNFYS
                     LAFLVIAIVGGYFWLLVVTAAAMTLFLLHQLEDMIKLRRVDPNNSILK"
     gene            4192..>4376
                     /locus_tag="VFJ67_08130"
     CDS             4192..>4376
                     /locus_tag="VFJ67_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290388.1"
                     /translation="MTDPGESDTKKNGNDSKFQADYAVKEYMPGLMTLVIKFVEKTNI
                     LFAKNGNLPIYEKKTFP"
BASE COUNT         1184 a         1060 c         1265 g          867 t
ORIGIN      
        1 ggaaggctcg tgaaggagag cggggcggaa tcggtcaagc tcgaaataaa cgaagactac
       61 gtagaaacgc tttacgcgat aaataaggcg gggatacccg tcataggcca cataggcctc
      121 tgcccccagt ccgtgctcga gatgggggga tataaggtgc aggggcgggg aagacacgag
      181 gcgaggaggc ttctcgattt agcaaaagcg atcgagagcg cgggcgcttt ctcgatactg
      241 atagagagca tacccaggac gctcgccgcg gagattacca aatcggtgaa gataccaact
      301 atcggcatag gcgcggggcc cggatgcgac ggccaggtgc tcgtgataaa cgacctcctg
      361 gggctctcgc ccgagccgat accgaagttc gtgaaaaaat acgcgaacct gagggaggtc
      421 gtggacaaat cgacgaaggc gttcatcgaa gacgtcaggt cgggcgcgtt cccctcagag
      481 gaacattcgt atgactgagc gcgggactgt taaagtcgta aacggcatag gggagatgca
      541 ggccctctcg aattcactca gaatcgaagg gaggatcatt tccttcgtcc cgacgatggg
      601 cgccctccac gagggccatc tgagcctcat gagggaggga aagaaacatg gggatgtgct
      661 tatcgcgagc gtcttcgtga acccgaccca gttcggaagg ggcgaggact ttcaaagcta
      721 cacgcgggac acggaaggcg atcttaaaaa gatggaatcg gcgggagcgg acatagcgtt
      781 cttcccccgc gcggacgaga tatacccgcc cgggtacgag acctacgtcg aggtaacgga
      841 gctcgagaag ccgctttgcg gcaggttccg tcccggacac ttcaggggcg tagcgacggt
      901 ggtattgaag ctctttaaca tcgtgaagcc ccacgtcgcg ctattcgggg aaaaggacta
      961 tcagcagctt cagataataa agaaaatggt cagggacctc aatctcgagg tcgggataat
     1021 cgggatgccc atcatcaggg aagagagcgg cctcgccctg agctcgagga atgcgtacct
     1081 cacccgtgaa gagagggaga aagcgacctg cctctcccgt gcgctcgggc agataaaaaa
     1141 gagcttcgac gcgggggaaa gagacacggc caggctcgcg ggagagggat actgcatgct
     1201 cgccggagct tctatcggcg aagtggacta catagaaata agggacggag agacgctcgg
     1261 ggagaagcgg ctcgcatctg cgggcgacgt cgcggccatc gccgtgcgta taggcggcgc
     1321 gaggctcata gacaataaag tgctttaaaa catattgacc gagaaggggg aattgtcatg
     1381 cacaggacac ttcttaaatc caaaattcac cgcgtgacgg tgacggacgc caatatcgaa
     1441 tacgagggga gcattacggt ggacagggac ctcatggacg cggccgactt ccaccactac
     1501 gagcaggtgc atatattcaa cgtatcgaac gggcacaggc tcgttacata cgtaatagaa
     1561 ggcgagcgcg gctcgggcga catatgcgtg aacggcgctg cggcccatct ggccagaaag
     1621 ggcgactgcc tcatcgtcgc gagcttcgca tcctatagtg agagcgagtg caaaacacaa
     1681 gtgcccaggc ttgtgtacgt ggacggacag aaccggatca cgcggatcgg ccccgagcag
     1741 aagacgttaa aggtcgccaa gagctgagcc cggccggccg tgagggaata gagctttggc
     1801 acgacggatt ttatccttat acttaatgaa aaaatggctt tccgtgacag ggcctggcgt
     1861 tatctgccgg ataagcatga attttcttaa aatctgcagc ctaacgccat aaaatacatc
     1921 ataaatattg acagaagcca ttctggaggt tataaatgtt agctagtatt aaaggaggag
     1981 aaaggataca ggattcgtat tctgcggatc cttctatatt acttctaaca aaaataacta
     2041 catatccttc tgatgcggag aatataatat gaaagctgta atactcgcag ctggtagagg
     2101 taaacgtctt tacccttaca ctaagtacat acctaaatgt ttactcgata tcggcggcga
     2161 gactattctc gagcaccaga tcaatcatct gcgcgactgc ggtatagacg aagttgtcat
     2221 cgtcgtgggt ttcgggttcg agaaggtaga aaatttcctg aggaattacg acggtctggg
     2281 gatgcgtata aaaacgctct acaacccctt ttaccagaca acgaacagtc tcatatccct
     2341 ctggatcgcg aggggagaga tggacgacga catagtcgtc atgaacgggg acgacgtatt
     2401 cgagatagaa gtccttgagc aggcgctccg gatgagggac gagaaaatat gcctccctat
     2461 caagaggaag tcgagctatg aagacgagga catgaaagtc gtaataaagg aaacgaagat
     2521 cgtcgatata agcaaaacgc tcacgaacag ggcctcggcc gagtcggtcg gcgtcagggt
     2581 gttcagggat acgggcgtcg agcttatgaa gagggcgatc gaagaggaaa tgaggacagt
     2641 aggggccgag aacaaatggt acatctccgc catacacagg cttattaaga aaggatacaa
     2701 ggtaaaatca ctcgacatca acgacctttt ctggatggac gtggactatc cgagcgacct
     2761 tttcaaggcg agattcaatt cgaataaatt cataaagaaa tcttatcagg aacgggtcct
     2821 aagagttgta gaaaccagtt aaaaaatgga ccatgcaata atcgttgccg ctggtacggg
     2881 aatagaagaa gaaaagttaa gcctgcatgg caagaccgta ttcggctctc ttcctcaaat
     2941 caaacgcctc gtcatcacag cccagagagc cggcattaaa aaattcacca taataaccga
     3001 aacgggcgat acctcgctga aggacctcct cgcgggcgat aggcgcatag agagcgatat
     3061 cggctggcac tccctgggat cggagataaa gctcgattcc gctcactgcc tcataataca
     3121 atctaacctc ataacgaccc ctcgctcgct cgcgaacttc atggagtcgg actgcgctca
     3181 ggacgaggtc gcggtcctcg tggactcgct cgacgacgca tggataaagg cgagcggcga
     3241 agaaatctcg gacgtctttt cctggggggg caaggcggta ggcgctttca tcgtgtcggg
     3301 caggctcctc gaaaaatcca taatgaattc gatgtcgctc aggaccttcg ttcaggagca
     3361 catcgggagg gacaaagtca agctcaggaa gttcacggac agttactgga tgaggctcga
     3421 ttcgggggag gacgccgcgg ccagggccga ggacctcatt ttcgcgcacg tcggcaagac
     3481 ggcgactgga tggctatcga ggaacatcaa cagcaagata tccctcccga cgagcaggct
     3541 gctcgtcagg actcccctca ctccgaacat gataagcgtg ctcataaaca taatcggcat
     3601 gatgtgcggg gtattctacg ccataggcca ccccgtgtgg ggcgcgctct gcatgcaggc
     3661 tgcgaccata ctcgaccggt gcgacgggga ggtcgcccgc gtgaagctca tggaaacgca
     3721 caggggacaa tgggtggaca ccatatccga ccaggtgacg gtgctttctt tcatgctggg
     3781 agtcccgatc gggtactacg tcatatcgaa gagtcccctc gcggtcatac tgggggccgt
     3841 aaacgtgtcc atatttatat ttttcgtcgt atggtcgttt tactttctga agaagtacac
     3901 caattccgga agccttgtaa cttacttcga tgtcgataaa cttgttgagg gtcggaaagc
     3961 gtcgctgatg cggagactca tcaagatcgt acgcccgatg ggcagaagaa atttctactc
     4021 cctggcgttc ctcgtgatcg cgatcgtcgg gggatatttc tggctgctgg tcgtaaccgc
     4081 cgccgccatg acgctgttcc tgctgcatca gctcgaggat atgataaaat tacgcagggt
     4141 tgatccgaac aacagcatac taaaataata tctcgcggga aggaaattga tatgactgat
     4201 ccgggcgaaa gcgatacgaa gaaaaacgga aacgacagta agttccaggc ggattacgcg
     4261 gtgaaggagt atatgccggg gctgatgacc ctggtcatca agttcgtcga gaaaacaaac
     4321 atccttttcg ccaaaaacgg caatcttcct atatatgaaa agaagacatt tccctg
//
LOCUS       DASSJH010000367         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_414345, whole genome shotgun sequence.
ACCESSION   DASSJH010000367 DASSJH010000000
VERSION     DASSJH010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414345"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..178)
                     /locus_tag="VFJ67_08135"
     CDS             complement(20..178)
                     /locus_tag="VFJ67_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290389.1"
                     /translation="MMTTLLVALGILASFGALFWLDRYYKDTGGDKSSEESDGGDDQD
                     SSPEYIQS"
     gene            complement(175..798)
                     /locus_tag="VFJ67_08140"
     CDS             complement(175..798)
                     /locus_tag="VFJ67_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290390.1"
                     /translation="MMRKLALIILPVLILAGCAQKSVYVPKSYRTKSATDEEKEKEKK
                     GAAIVTAGEEENKTNFNAPLDKVFAAAVESVQFLKWPIAFAEQQDGTIRLQEAYVYEK
                     NGKLFRSYTWPSSSDTSRSNVNYYLEKVGKYTPGTSETHFTQENLKVTVTKVSNEVTE
                     VKIDYYIHPYTFEGTIGYEVMSNGYIESIIIDHMKDILGGKPVAMER"
     gene            complement(1049..2056)
                     /locus_tag="VFJ67_08145"
     CDS             complement(1049..2056)
                     /locus_tag="VFJ67_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET7290391.1"
                     /translation="MRHLSRVVLLAVLLTAPGAFFSQTAFAVFFCNGQVATIVGTNGP
                     NLIFGTPGRDVIVGLGGNDDIRSGGGNDVVCAGGGNDVVRLGGGADQGFGGPGNDTIF
                     GQAGNDLVVGGIGNDTLRGNNGNDNVNGAAGSDDCDGGAGSNSILNCENGPAICQSPP
                     LTTNFSNIGVYFVDSFNAILVGLSSNGFDVVMVLSDIPFNGALLAVGGFPTSSTHCII
                     TEGAFDTDMDGNFSDEIVFGASGICDLPFNRTVLFLDNLVVAGEPLGFNITGECSEII
                     VINSATARGEQASTEAMTDGLIEGMSVISEEMTEDMRNEGGDNSGDAGLLKDFMNNME
                     E"
     gene            complement(2242..>2620)
                     /locus_tag="VFJ67_08150"
     CDS             complement(2242..>2620)
                     /locus_tag="VFJ67_08150"
                     /inference="COORDINATES: protein motif:HMM:NF019948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HET7290392.1"
                     /translation="SQKEMRALGRITANARGIPPREMIAEYSVGLMRALASPQRKPSS
                     INVLMHALGYFSGDLTGREKAYFLDLLTTYRNERIPLSAVLTVLESWVIKYGEDYLGR
                     QTFLEPYPRALMELSDSGKGRDF"
BASE COUNT          540 a          755 c          654 g          671 t
ORIGIN      
        1 gccctgaccg ccggacaggt tagctttgta tgtattccgg ggaggagtcc tgatcgtctc
       61 ccccgtctga ttcctcactg ctcttatccc cgcccgtgtc tttatagtat ctgtcgagcc
      121 agaagagcgc tccgaaagag gctagtatgc cgagagcgac gagtagggtc gtcatcacct
      181 ttccatcgct accggttttc cgcccagtat gtccttcatg tggtcaataa tgatcgactc
      241 gatgtacccg ttcgacatta cctcgtaacc gatagtccct tcgaacgtgt aggggtggat
      301 gtagtaatct attttgactt ccgtgacttc gttcgagacc ttcgtcaccg tcaccttgag
      361 gttctcctgc gtgaagtggg tctccgaagt gcctggagta tacttgccca ccttctcgag
      421 ataatagttc acgttcgatc tgctcgtgtc cgagctcgaa ggccacgtgt acgacctgaa
      481 cagcttgccg tttttctcat agacgtatgc ctcctggagg cgtatggtgc cgtcctgctg
      541 ctccgcgaaa gcgattggcc acttgaggaa ctgaacgctc tccacggctg cggcgaatac
      601 cttgtctaaa ggggcattga aattggtctt gttctcctct tcccccgcgg tcactatcgc
      661 ggcccctttc ttttcttttt ccttttcttc gtctgttgcg ctcttggtcc tgtaggattt
      721 cggaacatag acgcttttct gggcgcaccc ggccaatatg aggacgggca atataataag
      781 ggcgagcttt ctcatcattc tcctctgttc agataaaaat gtaacatatt attccataat
      841 ttccctcaaa attttagcgc acacacgtag tgtgtttcaa cggtctcttc caacccctct
      901 gcgcataccg gcaacgcagc ccaacccttt ctatttggct tcagatggtc tgattctggc
      961 attgcaagac ggtaaagctc ttgctcatgt ctggggaaag gacggggccc cgctcaggca
     1021 aggccccgca tcatgcagtc tggctaagct actcttccat gttgttcata aagtccttga
     1081 ggagacccgc gtcaccgcta ttgtcccctc cttcgttcct catgtcctcg gtcatctcct
     1141 cggagatgac ggacatgcct tcgattagac catcagtcat ggcttctgtc gaagcctgct
     1201 ctcccctggc cgttgccgag ttaattacga tgatctcgga acattcgccc gttatgttga
     1261 agcctagtgg ctcgcccgcc acgacgagat tgtccaggaa gagaacagtc ctgttgaaag
     1321 gcagatcgca gatacccgag gcgccgaata caatctcatc actgaagttt ccgtccatgt
     1381 cggtgtcgaa ggccccttcc gttattatgc agtgggttga ggaagtcggg aatccgccca
     1441 cggccaggag cgcgccgttg aacggtatat ccgacagcac catcaccacg tcgaacccgt
     1501 ttgaagacag tccgacgagg attgcgttaa acgaatcaac gaaatacacg ccgatgttgc
     1561 tgaaattcgt cgtgagcggc gggctctggc agatggcggg cccgttctcg cagttcaata
     1621 tactgttcga gccggctcca ccgtcgcagt cgtccgatcc cgctgcgccg ttcacgttat
     1681 cgttaccatt gttgcctctc agggtatcgt tgcctatgcc gcctacgacg aggtcgttcc
     1741 ccgcctggcc gaatatcgta tcgtttcccg gtccgccgaa tccctggtcg gcgcctccgc
     1801 ccagccttac aacgtcgtta ccgcctcctg cgcagacaac gtcgttaccc ccgccgctgc
     1861 gtatgtcgtc gttcccgccc aatccgacga ttacatcccg tccgggagtt ccgaaaataa
     1921 ggttggggcc gttcgtgcct actatggttg ccacctggcc gttacagaag aacacggcga
     1981 aggctgtctg tgagaaaaac gcgcccggcg ccgttaacaa aacagcgagc aatacaactc
     2041 tcgataaatg tctcatggaa atatcccccc gcttatatag gttatataat actataaaaa
     2101 cccaccgcct ccgtcccaat gatagcaaag tgaggtagaa gtgtaaatac attatttatt
     2161 taggaagctg cggagttagg gattaagatc ttctaaaagg gataattaaa tgttaacggt
     2221 tagtttcagg tgggccgtac ctcagaaatc ccttccctta cccgaatcgg aaagctccat
     2281 tagcgcccgc gggtagggct cgaggaaggt ctggcggccg aggtaatcct ctccgtattt
     2341 tatcacccag gactcgagta ccgtcagcac cgcggagagc ggtatcctct cgttcctgta
     2401 tgttgtcagg aggtcgagga aatacgcctt ctcccttccc gtgagatcgc ctgagaaata
     2461 cccgagcgcg tgcatgagga cgtttataga ggatggcttc ctctgtggag aggcgagggc
     2521 gcgcataagc cctacgctat attctgcgat catctctcgc ggtggaattc cccgcgcgtt
     2581 agccgtgatc ctgccgagcg ccctcatctc cttctggctg
//
LOCUS       DASSJH010000368         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1388057, whole genome shotgun sequence.
ACCESSION   DASSJH010000368 DASSJH010000000
VERSION     DASSJH010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388057"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1790
                     /gene="purL"
                     /locus_tag="VFJ67_08155"
     CDS             <1..1790
                     /gene="purL"
                     /locus_tag="VFJ67_08155"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET7290393.1"
                     /translation="GVVSGIAGYGNCIGIPTIGGEVYFDECYNGNPLVNVFALGITNK
                     DEIFRGYARGLGNPVIYVGSKTGRDGIHGAVMASETFTKEAEEKRPAVQVGDPFTEKL
                     LLEACLELFKTGAVVGIQDMGAAGLTSSSTEMAERAGTGIRIDIEKVPRREESMTPYE
                     VMLSESQERMLMVLEKGKEEVAERIFRKWGLDFSVIGEVTDTGRLVIAEEGKTVAEVP
                     ISLISEEAPVYDRPYSEPAYIKQTRKLDLSSVPVPKDLGRALLKLLSSPTLADKRRIW
                     EQYDHMVRTNTVVLPGSDAAIVRLKGTKKALAMTVNCNSTYCYLDPYTGGAAAVAESA
                     RNLVCSGARPLAITDCLNFGSPEEPGVMWQFKRAIEGISEASLKLNTPVVSGNVSFYN
                     QTGSVAVYPTPTIGMVGLIADVEKHITQWFKDDGDVIALLGETKEELGGSEYLKQVHG
                     IVAGEPPLLDLGAESCLLRALLEAADRGILKSAHDLSEGGLALSLAESCFAPEYTSGI
                     TVELPGTIRKDALLFSESQSRAVVSLDDKDLGILRKITESNNVPMSVIGKVGGAEFLI
                     GGLVDIPVSEARRAWATGFENSLKSHAKA"
     gene            1777..3195
                     /gene="purF"
                     /locus_tag="VFJ67_08160"
     CDS             1777..3195
                     /gene="purF"
                     /locus_tag="VFJ67_08160"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7290394.1"
                     /translation="MPKLKEECGVFGIYRNVEASNLTYLGLHALQHRGQESAGIVTSD
                     GVNLHNHRAMGLVSDIFTEEILSRLTGQNAIGHVRYSTTGSSQAKNMQPIVINYFRGA
                     LAIAHNGNLTNARTLREELEAEGAIFQSTTDTEVIVHLIAKSKEETLIQRIIEALAKC
                     KGAYSLLFLTPEAMIAVRDPYGFRPLVMGRLGDSVVFASETCAFDLIEAEYVREIEPG
                     EIVVVNDNGVESFKPFGDAKHSYCVFEYIYFARPDSFLTGRNVYQVRRALGKQLAIEQ
                     PADADIIVAVPDSGVPAAMGFSEQSGLPLELGLLRSHYVGRTFIEPQQSIRNFGVRLK
                     LNAIREVLDGKRVVIVDDSIVRGTTSRKIVKMIRAAGAKEVHMRISSPPTKFSCYYGI
                     DTPLKEELIANSLEIDEIRKYITSDSLGYLTLEGVLKAVDDTKKINGKESFCSACFSG
                     KYPVELTDLKKTKQKQLGLFGG"
     gene            complement(3228..3554)
                     /locus_tag="VFJ67_08165"
     CDS             complement(3228..3554)
                     /locus_tag="VFJ67_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290395.1"
                     /translation="MQILIQVVCSKGRSLRQEIVKDRKLDKYGLSVAEEKRPGRPHGW
                     AKVHSTGYDRHGAINIEWDAKTNVLLCRVVTRGAGKPNQIIGDFVDYLLRKFPRRIMA
                     INIIPR"
     gene            complement(3676..>4824)
                     /locus_tag="VFJ67_08170"
     CDS             complement(3676..>4824)
                     /locus_tag="VFJ67_08170"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7290396.1"
                     /translation="DVYIHGLIRDEKGHKMSKTRGNVIDPLDVIEENGADALRFSLTA
                     LATQGRDIKLSMSVIQGYRNFINKIWNASRFLIMNLGEYDDSVKVPDDELSTYDKWIL
                     TKLNETVKEVGDSFKGYEFDKAAGAIYKFFWSEYCDWYIESVKPVLYGDDPRGKQMTK
                     SVLVRVLKTALQLLHPIAPFISEELYQRLRAYGVELSAADGSTAESIMLSSFPECNET
                     QIYRDEHDEVELMKEIVVGIRNLRAVIGLHPSEKVSVVLLPESERARKQIEANRGFIL
                     SLASLSGFEIAKKDKPKKAIAQVIPGVEIFLPVEGLIDVGKETERIKKEIGKISKDLE
                     RTEKKLANPEFLERAPEEIVQKEREIFEELSFQIKKMEDVLSKLSEIG"
BASE COUNT         1113 a         1337 c         1361 g         1013 t
ORIGIN      
        1 gcggcgtggt atccggcata gcgggatacg ggaactgcat cggcattccc acgataggcg
       61 gcgaggttta cttcgacgaa tgctacaacg gcaaccccct cgtcaacgtg ttcgccctcg
      121 gcatcacaaa taaagacgag atattcaggg gctacgcgag ggggctcggg aaccccgtaa
      181 tctacgtggg ctcgaagacc ggaagagacg gcatacacgg cgcggtcatg gcgtcggaga
      241 cgttcacgaa ggaagccgag gagaagagac ccgccgttca ggttggagac ccgttcaccg
      301 agaagctcct cctcgaggcc tgcctcgagc tgttcaagac cggagccgtc gtcggcatac
      361 aggacatggg ggcggctggg ctaacgtctt catcgacgga gatggccgag agggccggca
      421 cggggataag gatcgacata gaaaaagtgc cgcggaggga ggagtccatg accccttacg
      481 aggtcatgct ctccgaatcc caggagcgga tgctcatggt gctagagaag ggaaaggagg
      541 aggtcgccga gagaatattc aggaaatggg gactcgactt cagcgtcatc ggggaggtaa
      601 cggacaccgg caggctcgtc atcgccgaag agggaaagac agtggccgag gtaccgataa
      661 gcctcatttc ggaagaggcc cctgtttacg acaggcccta cagcgagcct gcatacataa
      721 aacaaacgag aaagctcgat ttgtcctccg tacccgtccc gaaagacctc ggcagggcgt
      781 tgctgaaact cctgtcctcg ccgacactcg ctgacaagcg caggatttgg gagcagtacg
      841 accacatggt caggactaat accgtcgtgc tccccggctc ggacgcggcc atagtaagat
      901 taaaaggaac gaagaaagcg ctcgcgatga cggtcaactg caactctacc tactgctatc
      961 tcgatcccta caccgggggg gccgccgcgg tcgccgaaag cgcgaggaac ctcgtctgct
     1021 cgggggcccg gccgttagcg attacggact gtctcaattt cgggagcccg gaagagccgg
     1081 gagtgatgtg gcagttcaag agggcgattg agggcatatc ggaagcctct ctcaagctga
     1141 atacgccggt agtgagcggg aacgtgagct tctacaacca gacgggcagc gtagccgtct
     1201 atccgacccc cacgataggg atggtgggcc tgatagccga cgtcgaaaag catataacac
     1261 agtggtttaa agacgacgga gacgtcatag cgctcctcgg agagacgaaa gaggagttag
     1321 gcggcagcga gtatctgaag caggtccacg gcatagtggc cggtgagcct cctctactcg
     1381 acctcggggc agaatcgtgc ctcctccggg ctttacttga agccgcggat agaggtatac
     1441 ttaaatctgc gcacgatctt tcggagggag ggttagcact ttcactggcg gagtcctgtt
     1501 tcgccccgga atatacctct ggaattaccg tagagctgcc gggcacaatc agaaaggatg
     1561 cgctcttatt ttctgaatct cagtcacgag cggtggtttc actcgacgat aaggacttgg
     1621 gaatccttag aaaaattacg gaatctaata atgtaccgat gagtgtgatc ggaaaggtcg
     1681 gaggggcgga gttcttgatc gggggcctgg tcgatattcc ggtatcggaa gcaaggagag
     1741 cctgggcgac aggattcgag aacagcctca aaagtcatgc caaagcttaa agaagagtgc
     1801 ggcgtattcg gcatttacag gaatgtcgag gcatccaacc tcacatacct ggggcttcat
     1861 gcgctccagc acaggggtca ggagagcgcg ggcatcgtaa cctctgacgg cgtgaacctc
     1921 cacaaccacc gcgccatggg cctcgtctcg gacatcttca cggaagagat actctcccgc
     1981 ctcacagggc agaacgcgat cggccacgta cgctactcga ccacgggctc gagccaggcg
     2041 aagaacatgc agcccatcgt catcaactac ttccggggag cgctcgccat agcgcacaac
     2101 ggcaacctga caaacgcgag gacgctcagg gaagagctcg aagccgaagg cgccatattc
     2161 cagtcgacca cggacacgga agtcatcgta cacctcatag cgaaatcgaa agaagaaacc
     2221 ctcatacaga ggatcataga ggcgctcgcc aagtgtaagg gggcatattc tctcctcttc
     2281 ctcacaccgg aagccatgat cgccgtgagg gacccttacg ggttcaggcc tctcgtcatg
     2341 ggaaggctcg gggactccgt cgtgttcgcg tccgagacct gcgcattcga cttgatagaa
     2401 gccgagtacg taagggagat agagcccggc gagatagtag tcgtcaacga caacggtgtc
     2461 gaatcgttca agcctttcgg ggacgccaag cactcatact gcgtattcga atacatctac
     2521 ttcgcgaggc ccgacagctt cctcacggga aggaacgtgt atcaggtaag gagggctctt
     2581 gggaagcagc tcgccataga gcagcccgca gacgccgaca ttatcgtcgc ggtgcccgac
     2641 tccggggtgc ccgcagccat ggggttctcc gagcaatcgg ggctgccgct cgagctcggc
     2701 ctcctcagga gccactacgt ggggaggacg ttcatcgagc cccagcagtc gatcaggaac
     2761 ttcggcgtga gactcaagct taacgcgata agggaagtgc tcgacggaaa gagggtcgtc
     2821 atagtggacg actctatagt caggggtacg acgtcgagga agatcgtgaa gatgatacgc
     2881 gccgcgggcg caaaagaagt acacatgagg ataagctctc ccccgaccaa gttctcctgc
     2941 tattacggca tagacacgcc gctcaaagag gagctcatag ccaactcgct cgaaatagac
     3001 gagataagga agtacataac ctccgactcg ctcggttacc tcacgctcga aggagtgttg
     3061 aaggctgtag acgatacgaa gaagatcaac ggcaaggaga gcttctgctc ggcctgcttc
     3121 tcgggcaaat atccagtcga gctcaccgac ctcaagaaga ccaaacagaa acagctcggg
     3181 ctttttgggg gatagtcaaa aatatttgaa actgtgatta atcacaacta ccgcggaatg
     3241 atattgatcg ccattatcct gcgcgggaac ttacgtagca aataatcgac gaaatcaccg
     3301 atgatctggt tgggcttgcc agctcccctc gttacgaccc tgcaaaggag cacgttcgtc
     3361 ttagcgtccc actcaatgtt gatagcgccg tgtctgtcgt aacctgtgct gtgaaccttc
     3421 gcccagccgt gcggtctgcc cggacgtttt tcttccgcta cgctaagccc gtatttatcg
     3481 agcttccggt ctttaacaat ctcctgacgc aagctccgtc ctttcgaaca gaccacctgg
     3541 ataagtattt gcatgtatga ggtcccccca ttatccaaaa cctctaaaat tcagattata
     3601 aattatgtgt tttaacggtt caaatagact ggaagacgcc gatttggaag ttgcagtttt
     3661 caggcttgat taaatctatc cgatctcgct cagcttgctg agcacgtctt ccattttctt
     3721 tatctgaaag gaaagctcct cgaatatctc gcgctctttc tggacgatct cttcgggcgc
     3781 gcgttcgagg aattcggggt tagccagctt cttttcggtc cgttcgaggt ccttggaaat
     3841 ctttccgatc tctttcttta tcctctccgt ctctttcccg acgtcgatta gaccctcgac
     3901 ggggaggaat atctcgacgc cgggtatgac ctgcgctatc gccttcttcg gcttatcttt
     3961 ctttgcgatc tcgaaccccg acagagacgc gaggctaaga atgaagcccc tgttggcctc
     4021 tatctgcttc cgggcgcgct cgctctcggg caggagaacc acgctcacct tctcggacgg
     4081 gtgaagccct atcactgccc tcaggttcct tatgccgacg actatttcct tcataagctc
     4141 gacctcgtcg tgctcgtccc tgtaaatctg cgtttcgtta cattcgggga atgagctcag
     4201 cattatgctt tcggccgtgc tgccgtccgc agcgctcaac tccacgccgt aggcgcggag
     4261 cctctgatag agctcttcgg atatgaaggg cgctatcgga tggaggagct ggagcgcagt
     4321 cttgagcacg cgcacgagaa cgctcttcgt catctgtttg ccgcgcgggt cgtccccgta
     4381 gagcaccggc tttacggact cgatgtacca gtcacagtat tcggaccaga agaacttgta
     4441 tatggccccc gcggccttgt cgaactcgta gcccttgaac gagtccccca cctctttcac
     4501 ggtctcgttg agcttcgtga gaatccactt gtcgtaggtc gataattcat catccgggac
     4561 cttgacgctg tcgtcgtact cacccaggtt catgatgagg aagcgggacg cgttccagat
     4621 cttgtttatg aaattcctgt agccctgtat gaccgacatc gagagcttta tgtccctgcc
     4681 ctgcgtcgcg agcgcggtga ggctaaacct caatgcgtcg gccccgttct cctctatgac
     4741 gtcgagaggg tctatgacgt tccccctcgt cttgctcatc ttgtgcccct tctcgtcccg
     4801 gatgaggccg tgtatgtaaa cgtc
//
LOCUS       DASSJH010000369         6733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_623951, whole genome shotgun sequence.
ACCESSION   DASSJH010000369 DASSJH010000000
VERSION     DASSJH010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623951"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /locus_tag="VFJ67_08175"
     CDS             <1..1596
                     /locus_tag="VFJ67_08175"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET7290397.1"
                     /translation="IYINRDGTINVREDIEYDFQYAERHGIYRDIPYSYDYGYKRYSI
                     KLDVDKVTDFDGNPYKYEVSTGGGLVHIRIGDPDKTVTGVHGYRIEYTVRGAVAYFED
                     HDELYWNVTGDEWRVPIMKADAEVSFDGQTDEGVEADCYTGSRGSTAKDCDYEISAGR
                     VDFRAAGSLWAGQGFTVVVGLPKGMIEEPSGTKKTLWFLSDNWYFGLPFLTFFAIGYI
                     WYSRGRDPGGRGVITVRYEPPKDLTPAEAGTLVDERADISDITSTVIDLAVRGYLKIE
                     ETESTKFFFFTDRDYKLIKLKEPAPGELKPHEEKVFDGIFKGKQSVMVSELRDKFYTE
                     LPPIKKALYKELIDKKFFPADPEKIKGIYKWIGIIILILAFVLFSNFLLKISIALSGL
                     IILIASRYMPRKTREGTMLNEELLGFREFIDRAEKDRIEALAKNDPTLFDRVLPFALV
                     FGLEDKWANAFRDMYREPPSWYSSPNYRGSFSPNMFVSDLGRSLGVMNSSMSSTPRKS
                     GGSGLGGGGSSGGGFGGGGGGSW"
     gene            2001..6680
                     /locus_tag="VFJ67_08180"
     CDS             2001..6680
                     /locus_tag="VFJ67_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7290398.1"
                     /translation="MQEKSESKVRYKDKPRDVKKRSGVPGSAPHRSRKNPGLQSEQRP
                     SHDSFYEFSPLMCFSIDKKGIIKDVNSIGAEHLGYSVGELLDKSVLSVFYNDDRKNIL
                     AQIKKCLKDPSRTFTWEIRKIRKDGSIIWVRENAKAVIGGERKTQINIACEDITERKN
                     AEFLLNTENKVLEMIARNSSLNRILGYICKRVESHAREMLCSFLIMDEDGKHLRHGAA
                     PSLPREYIKAANGLQIGPCAGSCGTAAYLKKPVIVSDIETDPLWSDYKNAALKHGLRA
                     CWSTPILSTNENVLGTFAMYYTHPRSPSSHEKRIIETATHLARIAIERVKSQELVKRF
                     GEILEESLNEIYIIDSETGKFVQVNKGARINLGYTSEELLDLDPADINPDLTREFFGR
                     VETAISGERSKMMIEGRHRRKDGTLYPVETYLQMTSYGGKRVLVASAIDMTEARRREE
                     ELKGHVEQLSRKNRYESIILAVTESIHRSLDLQEVFENAVDAINKNVEGASNVVIFMV
                     EGNEAVLKADKGHTDWYIERVRRIPYPKGATWKTILDGKPRYVPDTDDDDYLGPAGKE
                     FGTKSYLSMPIQSEGVTVGCIHIHSQERNTFCIEDLDMLRIVATQLESAIKNAKQAEA
                     LKESEEDIKHKLAELSKKKRYEEITGTITRSVHSSIDIQEVLENAVDAMNKNIEGADN
                     VSIYFVEGKEAVIQTHRGYPEWFLNKTNRIPYPKGFTWKTILEGRLIYCPDVESDPVI
                     GQAGRDVGTKSYASMPIKYDGKTIGCININSFRKNAFDEDELGLLEIIASQIEIAVNN
                     ARQAEALKESEERYRTLFEQTPVGVYTYDKNLIITNVNERHAEIMQSSRDKIIGLDIN
                     DLSDKSFNYIHEGAVRGLESCHEGYYQSTTGTAIPFLRVSCAPLRDAQENIVGGMSVV
                     EDITERKKAEEALIQSQQDYESLVNAIEGIVWEGKINPLRFTFVSKRAKRMLGYPLED
                     WTNGKEIWVEKLHPEDKEWVLSFCTLETSLKRDHVIEYRMISADGRTVWLRDIVSVIV
                     ENDEVTGLRGIMIDITDLKTAEMKFKESADRYRTLVENIEDLIVETSADGRFIYLSPK
                     HEETLGYKPEELSGRNIFENIHPDDIPLAMEEFSRVVSSQTSGKAVFRYRHKNGSWRW
                     FESTGQTVETASGELRCVIVSRDITDRRRLEEELFKAEKLESLGVLAGGLAHDFNNLL
                     TVILGNISISKMKLDPKEKIYGRLSEAENASFRARDLTSQLLTFSRGGAPVKEFVQSL
                     GELIVDTANFVVSGSKVKCEFDIDDGLWPVEVDEGQISQVIHNLIINSDQAMPDGGVI
                     NVSARNTELGTDNGMHLKEGRYLKITIEDNGIGMTDDLIEKIFDPYFTTKQKGSGLGL
                     ATVYSIVRNHEGRIGVSSKIGAGTRFDIYLPASVGKPVSGTGGGYLQAGYGKILLMDD
                     DELVREAAGQMISQLGYEVEYASDGLEAIEKYRDAMEIGSPYDAVMIDLTVPGGMGGK
                     EAQKRLMEIDPSVRSIVSSGYSNDPVMSRFDEYGFKSVLTKPYSIEVLSKTLHGVLNH
                     RADTKNR"
BASE COUNT         1927 a         1651 c         1909 g         1246 t
ORIGIN      
        1 atatacataa accgcgacgg tacgataaac gtccgcgaag atatcgagta cgacttccag
       61 tacgccgaaa ggcacggcat ctaccgggac atcccctaca gctacgacta cgggtacaag
      121 agatacagca ttaagctcga cgtcgataag gttacggact tcgacggaaa cccgtataaa
      181 tacgaagtca gcaccggagg cggcctcgtc catataagga taggcgaccc ggataaaacc
      241 gtcactggag tgcacgggta caggatcgaa tatacggtga ggggcgcggt cgcgtatttc
      301 gaagaccacg acgagctcta ctggaacgtc acgggcgacg aatggagggt cccgataatg
      361 aaagctgacg cagaggtctc tttcgacggt cagacggacg aaggcgtgga ggcggattgt
      421 tatacgggat cgaggggctc gacggcgaag gactgcgatt atgagatctc ggcgggaagg
      481 gttgacttcc gggcggcggg ctcgctctgg gcagggcagg gattcaccgt ggtggtcgga
      541 ctgccgaagg gtatgatcga ggagccgtcc gggacgaaga aaacactgtg gttcctctcc
      601 gacaactggt atttcgggct gccgttcctg acattcttcg ccataggata catttggtac
      661 agccgcggaa gagacccggg aggcaggggc gtcattacgg tacgctacga gccgccaaaa
      721 gaccttacgc cggctgaagc ggggacgctc gtcgatgaga gggccgacat atcggacatc
      781 acgtccacgg ttatagacct cgccgtgagg ggatacctca agatcgagga aaccgaaagc
      841 acgaaatttt tctttttcac cgacagggac tacaagctca taaagctcaa ggagcccgcg
      901 cccggtgagc tgaaacctca cgaggagaaa gtcttcgacg gaatattcaa gggcaagcag
      961 agcgtcatgg tctcggagct cagggacaag ttctacacgg agctcccgcc aatcaaaaaa
     1021 gcgctttata aggagttaat agataaaaaa ttcttcccgg ccgaccctga aaagatcaag
     1081 ggcatatata agtggatcgg gataataata ctcatactcg ccttcgttct gttcagcaat
     1141 ttcctgctta aaatctcgat cgcgctttcg gggctgataa tcctcattgc gtcgagatac
     1201 atgcccagaa agaccaggga agggacgatg ctcaacgagg agctcctggg attcagggag
     1261 ttcatagaca gggccgagaa ggacaggatc gaggccctgg ccaaaaatga cccgacgcta
     1321 ttcgacaggg tgcttccctt cgcgctcgtc ttcggactcg aagacaagtg ggcaaacgcc
     1381 ttcagggaca tgtacaggga accgccctcc tggtactcct cccctaacta cagaggatcg
     1441 ttctctccta acatgttcgt ctccgatctc ggcaggagcc tgggcgtgat gaacagctcc
     1501 atgtcctcga cgccgaggaa atcgggcggg agcggattgg gcggaggcgg gtcgagcgga
     1561 gggggcttcg gcgggggcgg aggcgggtcg tggtgatcaa gtgaacggcc cgggtacagg
     1621 acaactcaaa ccgtatatag acacgtgcag aatactcatc tcccggcaga cgctcatacc
     1681 aaggagcgtt gacgcaatcc tgcggacgca tcagtaatca attgtaatca ttcattatat
     1741 gtacctgcaa agattccgcc actttaaacg gaaagggatt ataatttaat taacgtgatt
     1801 cggttaacag gcccgacagc tctacaccgg cctgaggggg gaaccgtaaa caaccggaga
     1861 gaaataagcg ccgctgaaat gaatccgttt gaaaggcccg cgcatagctc cgcccgatgc
     1921 ccgatttcac agcggcgctc cggaacatac accggccgtt aaatatcccc tcccgagcgt
     1981 ccggaaaagg ttgagtgaga atgcaggaga agtccgagtc gaaagtcagg tataaagata
     2041 aaccccgcga cgtcaaaaaa cgatccggag tacccggcag cgctccacac agatcccgga
     2101 aaaaccccgg cttgcagtcc gaacaaagac cctcacacga ttcattttat gaattcagcc
     2161 cgctaatgtg cttttcgatc gataaaaaag ggatcatcaa agacgtgaat tcgatcggcg
     2221 ccgaacacct gggctactct gtcggggagc tcctggacaa gtccgtcctg agcgtctttt
     2281 ataacgatga caggaaaaac atactcgcac agattaaaaa gtgcctgaaa gaccccagcc
     2341 ggacattcac atgggagatt agaaagatca gaaaggacgg cagcatcatc tgggtccggg
     2401 aaaatgcaaa agctgtaatc ggcggtgaaa gaaaaaccca gataaatatc gcctgcgaag
     2461 acataacgga aagaaagaac gccgagttct tgctcaatac cgagaacaag gtgctcgaga
     2521 tgatagcccg caactcgtcg ttaaaccgga tcctcggtta tatatgcaag cgtgtcgaga
     2581 gccatgcccg ggagatgctt tgttcttttc taatcatgga cgaagacggg aaacatctca
     2641 ggcacggagc ggcgccgagc ctgcctaggg aatatataaa agccgcaaac ggattgcaga
     2701 ttgggccgtg cgcgggctca tgcgggacgg ccgcttatct gaaaaagccc gtgatcgtga
     2761 gcgacatcga gaccgatccc ctctggtccg attacaagaa cgcggcattg aagcacggct
     2821 taagagcatg ctggtccact ccgatattat cgaccaatga gaatgtactg ggaacttttg
     2881 ccatgtatta cacgcacccc cgttctccca gctctcacga aaaaaggatt atcgaaaccg
     2941 cgactcacct ggcccgtata gccatcgagc gcgtcaaatc tcaggaactg gtcaagcgtt
     3001 tcggcgaaat cctcgaagaa tccctaaacg aaatctatat aatagattcg gagacgggaa
     3061 agttcgttca ggtgaacaaa ggcgccagaa tcaacctcgg atacacatcc gaagaattac
     3121 tggacttaga ccccgccgat attaaccccg atctcacacg agagttcttc ggcagggtgg
     3181 aaacagcgat ttccggcgaa cgaagtaaaa tgatgatcga aggcagacac aggcgtaagg
     3241 acggcacgct gtatccggtt gaaacgtacc tccagatgac gagctacgga ggcaagagag
     3301 ttcttgtcgc ctcagccatc gatatgacgg aggccagaag aagggaagaa gagctaaagg
     3361 gccacgtaga gcagctatcc aggaaaaaca ggtacgaatc catcatactc gccgtgacgg
     3421 aaagcataca ccgctcgctc gacttgcagg aagtgtttga gaacgcagtg gacgcgatta
     3481 ataaaaacgt ggagggagcg agcaatgtcg tgatattcat ggtggagggt aatgaggccg
     3541 tattgaaagc cgataaagga catacggact ggtatatcga gcgtgtaaga agaatccctt
     3601 atccgaaagg agctacctgg aagaccatac tcgacggcaa acccagatac gttccggata
     3661 cggatgatga cgactatctg ggtcccgccg gaaaggagtt cggcacaaag agctacctgt
     3721 caatgccaat ccagtcggag ggggtgaccg tcggctgtat tcatatacac tcacaggaaa
     3781 ggaatacgtt ttgcatagaa gacctggaca tgctccggat cgtggcaaca cagcttgaat
     3841 cggccatcaa gaacgcgaag caagccgagg cgctgaagga gtccgaggaa gatattaaac
     3901 ataaactggc cgaattatcc aagaagaaaa gatacgaaga gataacaggc acgatcacaa
     3961 ggagcgtcca cagttccata gacatacagg aggtgcttga gaacgccgtc gacgcgatga
     4021 acaagaacat agaaggcgcg gacaacgtct caatttattt cgtcgaggga aaagaagccg
     4081 ttatccagac tcacagggga taccccgaat ggttcctcaa caaaacgaac cggatcccgt
     4141 atccgaaggg ctttacgtgg aagacgatac tggaagggag attgatctat tgtccggatg
     4201 tagagagcga tccggtcatc gggcaggcgg ggagagatgt gggaaccaaa agctacgcct
     4261 caatgccgat caagtatgac ggcaagacaa taggctgcat aaacataaat tcattcagga
     4321 agaatgcatt cgacgaggac gaactcggcc tgctggaaat catcgcttca caaatagaga
     4381 tcgcggtcaa taacgccagg caggcggagg ctcttaaaga atccgaagaa agatacagga
     4441 cactgtttga gcaaaccccc gtgggagtct atacatacga caagaacctc atcattacca
     4501 acgtgaacga aaggcacgcg gaaatcatgc agtcctcacg agataaaatt atcggtctcg
     4561 acataaacga cctcagcgac aagtcattca actatataca cgaaggtgcg gtccggggac
     4621 tcgaatcctg ccacgagggc tattatcagt cgacgacagg gaccgcaatt ccgttcctgc
     4681 gcgtttcatg cgcaccgctg agggacgccc aggaaaatat tgttggcggc atgtcggtag
     4741 tggaagacat aaccgaacgc aaaaaagcag aagaggcgct aatacaatcc cagcaggatt
     4801 atgaatccct cgtcaatgcg atagaaggga tagtatggga agggaagatc aatcccctgc
     4861 gctttacctt cgtcagcaaa cgagcgaagc gcatgctggg ctacccgctg gaagactgga
     4921 caaacgggaa agaaatctgg gtcgagaaac tgcacccgga ggacaaagaa tgggtcctga
     4981 gtttctgtac tcttgaaacc tcactgaaga gggatcacgt gatcgaatac agaatgatct
     5041 cggctgacgg ccggactgtc tggctcaggg acattgtctc ggtcatcgtc gagaacgatg
     5101 aggttacggg cctccggggc ataatgattg acatcaccga tctaaagacc gccgagatga
     5161 agttcaaaga gagcgcggac aggtacagga cgctcgttga gaatatagaa gacctcatcg
     5221 tggaaacaag cgcggacggc aggtttattt atttaagccc gaaacacgag gaaacgctcg
     5281 ggtacaagcc cgaagagctc tccggcagga atattttcga gaacattcat ccggacgaca
     5341 ttcctctcgc gatggaagaa ttcagcaggg tggtgagcag ccagacctcg ggtaaagcgg
     5401 tcttcagata cagacacaag aacggcagct ggagatggtt cgagagcacg ggccagaccg
     5461 tcgagaccgc gagcggcgag ctcaggtgcg tcatcgtatc ccgggacatc accgacagga
     5521 ggagattaga ggaggagctt ttcaaagcgg agaagctcga gtctctcggc gtcctcgcgg
     5581 gggggttagc acacgacttc aacaacctcc tgaccgtcat cctcggaaat atttcgattt
     5641 cgaaaatgaa gctcgacccg aaagagaaaa tatacgggag gctgtcagag gcggaaaacg
     5701 cctctttcag ggcgagggac ctgacgtctc aactcctgac gttctcaaga ggcggggctc
     5761 cggtgaagga gttcgttcag tccctcggcg agctcatcgt cgatacagcc aatttcgtgg
     5821 tcagcggatc gaaggtcaaa tgtgaattcg atatagacga cggcctgtgg cctgtggaag
     5881 tcgacgaagg ccagatcagt caggtcatcc acaacctcat aatcaattca gaccaggcga
     5941 tgccggacgg aggcgtaata aatgtgagcg cccggaacac agagctcgga acggacaacg
     6001 ggatgcatct aaaagagggc agatacctca aaatcacgat cgaagacaac gggataggga
     6061 tgacggacga tctgatagaa aagatattcg acccctattt cacgactaag cagaagggga
     6121 gcgggctcgg gcttgctacc gtgtattcca tagtgaggaa ccatgagggc cgcatagggg
     6181 tttcttcgaa aatcggcgcg ggcacgcgtt tcgatatcta tctgcccgcg tccgtgggga
     6241 agcccgtgag cgggaccggc ggcgggtatc tccaagcggg atacgggaaa atactcttga
     6301 tggacgatga tgagctcgtg agggaagcgg ccgggcagat gatcagccag ctcgggtacg
     6361 aagtagagta cgcatcagac ggactggaag ccatagaaaa atacagagat gcgatggaaa
     6421 tagggtcgcc ctacgacgcc gtaatgatag accttaccgt tccgggaggg atgggcggaa
     6481 aggaggcgca aaaaaggctt atggaaatag acccctccgt caggtctata gtctcgagcg
     6541 gttattcgaa cgaccccgtg atgtcgagat tcgacgaata cgggttcaaa agcgtgttaa
     6601 cgaagccgta tagcatcgaa gtgctgagca agaccttaca cggtgtactg aaccaccggg
     6661 ctgatacaaa aaaccgctga aagaacttaa caacacccgg ctgaatgcgg gcccgcttcg
     6721 gcccttatgt cag
//
LOCUS       DASSJH010000370         4609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1274024, whole genome shotgun sequence.
ACCESSION   DASSJH010000370 DASSJH010000000
VERSION     DASSJH010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274024"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFJ67_08185"
     CDS             <1..615
                     /locus_tag="VFJ67_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7290399.1"
                     /translation="QQFDWEVPDWIIIPGGNLGNISALGKGFLIMKELGLIDKLPRLV
                     CAQAEHANPLYLSYLKNFEEFHPVKAKTTLANAIQIGNPVSVNKAIRIIKEFDGIVEQ
                     ASEEELSNACALVDRTGTFNCPHTGVALAAFLKLRERKVFKPSDRIVIISTAHGLKFV
                     EFKIGYHKGELEGVFSKYANKPVELPADYNAVTDAIFKVIEKRA"
     gene            complement(675..1478)
                     /locus_tag="VFJ67_08190"
     CDS             complement(675..1478)
                     /locus_tag="VFJ67_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290400.1"
                     /translation="MNSAKAVSGTVPYSFFLEHPYSSTGLSGNLEIAPVDGGGELIMN
                     RFRADLNLGQNILGIYAKFPFSGVTSDVFGSGDYDFGNIGLGTKYALLSSNNAALTLG
                     FEAILPTTGNNLGALGARSYFRDFAYFTDDAYTFVPYGVFALGGDVLAFQANVDFDIM
                     TNAEKANESQFDTGGDSAELIIKYGAAASVTPRFNLPFSTSFLLELLLASSTTFEKNV
                     TGVYVTPGVRFGGIKYSFGAGVQVPFGSDEISDFTSVDFVIDMKVRFGG"
     gene            1736..2374
                     /gene="thiE"
                     /locus_tag="VFJ67_08195"
     CDS             1736..2374
                     /gene="thiE"
                     /locus_tag="VFJ67_08195"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7290401.1"
                     /translation="MKRMLKGLYVITDKKLIPRDRFADTVEEAIRGGASVVQLREKDT
                     REDEVIEIGKTLLGLTRKYGVPLIINDSAELARVIGADGVHLGEDDPMVAHAREVLGR
                     DKIIGVSCYGKIERGINAERDGADYVAFGTPFFTPTKPERTPTSFDVLKEAVSRIKSI
                     PIFAIGGITPENAESVLETGVDGIAVITAVFGSGDPGKAAREIASIAERYSK"
     gene            complement(2398..>4609)
                     /gene="leuS"
                     /locus_tag="VFJ67_08200"
     CDS             complement(2398..>4609)
                     /gene="leuS"
                     /locus_tag="VFJ67_08200"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET7290402.1"
                     /translation="RGVHPAVWTYDNIAQMKAQLKQLGFSYDWTREIATCDPSYYKWE
                     QMVFTRMYEKGLAYRKSTTVNWCPSCETVLANEQVEDGLCWRCGSVVELKVMEGWFFK
                     TTEYTDELLEYTEKLKDGWPERVLAMQKNWIGKSVGAEIDFPLIEPVGGLDRLGIFTT
                     RPDTVFGVTYMSIAPEHPLAAELIKGKPEEKKALEFIKRVTKQSSLERIAEGAKKEGV
                     FTGAFAVNPFNGNRVPIYIANFVLVEYGTGIIMCVPAHDQRDFEFAKEYGLPIVPVIE
                     PEGTHLKAEDMTSAYEEHGVMVNSGQFSGLPSEEGKTKVIEYVEEKGIGLRRVNYRLR
                     DWGISRQRYWGAPIPIIYCDKCGIVPVPDKDLPVELPLDIEFTGKGGSPLSKAESFVN
                     TVCPGCGGKAKRETDTMDTFVESSWYFLRYVSPHYEEGMFDKKEVGYWLPVDRYIGGI
                     EHAILHLLYARFYTKVLRDLEMCSLDEPFTNLLTQGMVIKDGAKMSKSVGNIVDPDDM
                     IKKYGADTVRLFMLFAAPVQKDLDWSDEGIEGAFRFLSRLWRLISDRFPKIEPLKGKT
                     PDVNKLSGSGKDLLTKVHKTIKKVTDDLEKFQFNTAIAAVMELLNDTSRFESSGGEDE
                     KVVRESVEVMVRLLYPIAPHVAEELWRELGYDNSLVDEPWIEWNKELVAGAAITVVVQ
                     VNGKVRAQLSMDADVSDEDMREAAFADEKVKSYLAGKEPKKVFVVPKKIVNIVV"
BASE COUNT         1025 a         1283 c         1239 g         1062 t
ORIGIN      
        1 cagcagttcg actgggaggt tccggactgg atcattattc cgggaggcaa cctgggaaac
       61 atatcggcgc tcggcaaagg gttcctcatt atgaaggagc tcggcctcat agacaagctg
      121 ccccgtctcg tctgcgccca ggcggagcac gcgaaccctt tgtacctgag ctatctgaaa
      181 aatttcgagg agttccatcc ggtcaaggcg aaaacgacgc tcgcgaacgc aatccagatc
      241 gggaacccgg tgagtgtgaa caaggccatc aggataataa aggaattcga cggtattgtg
      301 gagcaggcga gcgaggaaga gctttcgaac gcatgcgctc tcgtcgacag gaccgggact
      361 tttaactgcc ctcacacggg cgttgcactc gccgcattcc tgaagctgcg ggagagaaag
      421 gtctttaagc cgtctgacag gatagtgata atatccaccg cgcacgggct taagttcgtc
      481 gagttcaaga tcgggtatca caagggcgag ctagaaggtg tattttccaa atacgcgaac
      541 aagccggtcg agctgcctgc cgattacaac gccgtgaccg acgcgatatt caaggttatc
      601 gaaaagcggg cttagtaagt gaggccgcga cgcgggtata cgccgggcac acacaatcca
      661 aagatttttt aatatcagcc tccgaacctc accttcatat cgataacgaa atcgactgag
      721 gtgaaatcgc ttatctcgtc gcttccgaac ggaacctgca cacctgcgcc gaagctgtac
      781 ttaatgccgc caaaccttac gccgggcgtg acgtatacgc cggtgacgtt cttctcgaac
      841 gtggtggaag acgcgagcag gagctcgagg agaaatgagg tcgagaacgg caggttgaac
      901 cgcggagtca cgctcgcggc agcgccgtat ttaatgatca gctcggcgct gtctccgccc
      961 gtatcgaact ggctctcgtt cgctttctcg gcgttggtca tgatgtcgaa atccacgttc
     1021 gcctgaaaag cgaggacgtc tccgcccagc gcgaacactc cataaggaac gaatgtatat
     1081 gcatcgtccg tgaaataggc gaaatcgcgg aagtacgagc gcgcgccgag cgctcccagg
     1141 ttattccccg tcgttggaag aatcgcctcg aaccctaacg tgagcgcggc gttgttcgag
     1201 ctgaggagag cgtatttcgt acctaatccg atgttcccga aatcgtaatc cccgctcccg
     1261 aagacgtcgc tcgtcacgcc cgaaaacggg aacttggcgt atatgcccaa tatgttctga
     1321 cccagattga ggtctgcacg aaacctgttc ataatgagct cgccgccgcc gtccacaggg
     1381 gcgatctcga ggttcccgct cagcccggtc gagctgtagg gatgctccaa gaaaaacgag
     1441 tacgggaccg tgccgctcac agctttagcc gaattcaaag gtgcgcatac gaaaataacc
     1501 gcagtcagta gtaagcagag aataggcgca gagcatgcct ttttaatcct catttcgcgt
     1561 tttcctcctc caggtttgcc aagagtttat tataatgggc ccactatttc aaaccgtctc
     1621 gaagattctt gcataacgcc ctgatataaa atcaatttat cgccgaataa tcctgtcgcg
     1681 cgtatatcat aaaatatttg ccttaatatc aattcaccgc taaatttcta atattatgaa
     1741 gcggatgctg aaagggctat acgtgatcac tgacaagaag ctcataccga gggacaggtt
     1801 cgcggatacg gtagaggagg cgatccgggg cggggcgagc gtcgtccagc ttagggaaaa
     1861 ggatactcgg gaggacgagg tcatagagat tgggaaaacc ctgctcggat tgacaagaaa
     1921 atacggcgtt ccgctaataa ttaatgattc agccgagtta gcgagagtaa taggggcgga
     1981 cggcgttcat ttaggggagg acgacccgat ggtcgcccac gcgagggaag tgctcggccg
     2041 ggacaagata ataggcgtct cctgctacgg gaagatagaa agggggatca acgccgagcg
     2101 tgacggagct gattacgtcg ccttcggtac gccatttttc accccgacca agcccgagag
     2161 gacccctaca tcatttgacg tgcttaaaga agcggtatcg agaattaaaa gcattcctat
     2221 attcgcaatc ggcgggatta cgcctgaaaa cgccgagtca gtcctcgaaa caggggtcga
     2281 cggcatcgcc gtcatcactg ccgtattcgg ctcgggagac cccggcaagg ccgcgcggga
     2341 gatcgcatcc atcgccgaaa ggtactctaa ataaagtagc tgtttccgta agcactctca
     2401 gaccactata ttgactatct tcttggggac gacgaagacc ttcttaggct ctttcccggc
     2461 gagatagctc tttacctttt cgtcggcgaa ggcggcctct ctcatgtctt cgtcgctcac
     2521 gtcggcgtcc atcgagagct gcgccctgac cttaccattc acctgaacga cgactgttat
     2581 agcggctccg gctacgagct ccttattcca ttcgatccac ggctcgtcga cgagggaatt
     2641 atcgtatccg agctcccgcc acagctcctc cgcaacatgg ggagcgattg ggtaaaggag
     2701 cctcaccatc acttcgaccg attctctcac aaccttctcg tcctcgccgc ccgaagactc
     2761 aaaccgggag gtatcgttga gaagctccat tacggcggcg atcgctgtat tgaactgaaa
     2821 cttttcgagg tcgtccgtca ccttctttat agtcttgtgc actttggtga ggaggtcctt
     2881 gccggagccg gataatttat ttacgtccgg cgtcttgccc ttgagcggct cgatcttcgg
     2941 gaacctgtcg gagatcaggc gccagagcct gctcaggaac ctgaacgctc cttctatacc
     3001 ctcgtcgctc cagtcgagat ccttctgcac gggggcggcg aagagcatga ataggcgcac
     3061 ggtgtccgcc ccgtactttt ttatcatgtc gtccgggtcg acgatgttgc cgacggactt
     3121 cgacatcttt gccccgtcct ttatgaccat cccctgtgtg agtaggttcg tgaagggttc
     3181 gtcgagtgag cacatttcga ggtctctaag aacctttgta tagaacctcg cgtagagcag
     3241 gtgaagtatc gcgtgctcga tgccgcctat gtacctgtcc acagggagcc agtacccgac
     3301 ctcttttttg tcgaacatgc cttcctcgta atgaggagat acgtacctga ggaagtacca
     3361 ggaggactcg acgaacgtgt ccatcgtgtc ggtctcacgc ttagccttgc cgccgcaccc
     3421 gggacataca gtattgacga aactctccgc tttcgataac ggagatccgc ccttccccgt
     3481 aaactcgatg tcgagcggaa gctcgacggg aaggtctttg tcgggaacgg ggacgatgcc
     3541 gcacttatcg cagtagataa taggtatggg cgcgccccag tacctctggc gagagatgcc
     3601 ccagtccctg agcctgtaat tgaccctcct caagcctatt cctttttcct ctacatattc
     3661 tatgaccttt gtcttgcctt cctcggaggg cagcccggag aattgtccgg aatttaccat
     3721 gacgccatgt tcctcgtatg ccgaggtcat atcttcggct tttagatgcg tgccttcggg
     3781 ctctatcacg ggaactatgg ggagcccgta ctccttcgcg aattcgaagt cccgctggtc
     3841 gtgggcgggg acgcacatga tgatgcctgt gccgtactcg acgagcacga agttggctat
     3901 gtatatgggc acgcgattgc cgttgaatgg atttacggcg aaggctcctg tgaagacgcc
     3961 ctctttctta gccccttccg ctattctttc aaggctgctc tgcttagtaa cccttttaat
     4021 gaattcgagc gccttctttt cctcgggctt tccttttatc agctccgccg cgagcgggtg
     4081 ctcgggagct atgctcatgt acgtgacgcc gaacacggta tccggccgcg tcgtgaatat
     4141 gccgaggcgg tccagaccgc cgacgggctc aatgagaggg aaatctattt ccgctccgac
     4201 gctctttccg atccagttct tctgcattgc gagcaccctt tcgggccagc cgtctttgag
     4261 cttttcggtg tactccaata attcatcagt atattctgtc gttttgaaga accacccttc
     4321 cataaccttg agctcgacta cggagccgca gcgccagcag agcccgtcct ccacctgctc
     4381 gttcgcgagc acggtctcgc aggaagggca ccagttgacg gtggtcgatt tccggtacgc
     4441 gagccctttc tcgtacatcc gggtgaacac catctgctcc cacttatagt aagacgggtc
     4501 gcacgtggcg atctccctcg tccagtcgta gctgaagccc agctgcttta gctgagcctt
     4561 catctgtgcg atattgtcgt aagtccatac ggcggggtgc acgccccgg
//
LOCUS       DASSJH010000371         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1389424, whole genome shotgun sequence.
ACCESSION   DASSJH010000371 DASSJH010000000
VERSION     DASSJH010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389424"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /gene="murA"
                     /locus_tag="VFJ67_08205"
     CDS             complement(<1..1044)
                     /gene="murA"
                     /locus_tag="VFJ67_08205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7290403.1"
                     /translation="MEKIVVEGGKRLEGEIVVSGAKNAVLPLMAACILSEGKNIITNV
                     PDLADVRTMGKLLQILGADVEYDRGTLSIDTSNLQSWEAPYDLVKTMRASVLVLGPLT
                     ARFGKSRVSLPGGCAIGERPIDQHLKGLSILGSKIVIEEGYVETNAPRLRGGIVPFDV
                     STVTGTENLILSSVLAEGETILLNAACEPEVTDLATALNQMGADITGIGTDIITIRGV
                     KKLSPLKSYEVMPDRIEAGTYMIASVLTKGDLMIKNCRFENMGALIGKLIEIGAQITR
                     ENGGIRVKSNGVLKSTDITTLPYPGFPTDMQAQMMTLMSTSDGLSVLTETIFPQRFLH
                     AGELRRMGADIKLI"
     gene            complement(1032..1883)
                     /gene="prmC"
                     /locus_tag="VFJ67_08210"
     CDS             complement(1032..1883)
                     /gene="prmC"
                     /locus_tag="VFJ67_08210"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7290404.1"
                     /translation="MHLRDLYQLGKKNMRLHLIENPALEASILLSKSSAIEDVSEIYA
                     HPEKELDQVKVDEFNRLLERRIKNEPIAYITGEKEFYSRAFSVSRDVLIPRPETELLV
                     EEALGTIADIGDPAILDVGTGSGCIAVTISCEKPGSRVFAADISGGALSLARANAERH
                     GAGSGMFFVLGSLAEPFRDESFDVLVSNPPYIPEAEFENLAPDVRDYEPRSSLIGGED
                     GLYFIRKIISGAKRVLKSGGWCLLEIGVGQSPEVIKLFEEASFNNISCVRDINDIERL
                     VKAQWKK"
     gene            complement(2064..2726)
                     /gene="phoU"
                     /locus_tag="VFJ67_08215"
     CDS             complement(2064..2726)
                     /gene="phoU"
                     /locus_tag="VFJ67_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7290405.1"
                     /translation="MVRLEEEISKLKKMLFEMATSVEEMIAKSIKALKDRNMIMAEEV
                     IKSDAKINEMEIEIDNQCIKILALYHPEAEDLRTVSMIMKINNDLERIGDHAVNIAEK
                     TIYLADKPPVKPLIDIPRMADKAIQMLQESLDAFVNKDAQLAIDVCKRDDEVDALEPQ
                     IVRELVTYMIADPQTIDRSLTIILIAREIERVADLATNIAEDTYYIVSGKSLKHHSLE
                     KT"
     gene            complement(2714..3199)
                     /locus_tag="VFJ67_08220"
     CDS             complement(2714..3199)
                     /locus_tag="VFJ67_08220"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET7290406.1"
                     /translation="MPSEGKKTVIFICTHNSARSQMAEGILRHLYGDRFDVHSAGTRP
                     SRVNPYAVRVMDEIGIDITEHSSKSLDAYEGAGFDCVVTVCDHANEACPVFPGGGRHI
                     HRGFRDPAAFRGRDEEILAGFRTVRDEIKDWIESEFGVISCGARKEDSEPRSEIKIWS
                     D"
     gene            complement(3262..>3636)
                     /locus_tag="VFJ67_08225"
     CDS             complement(3262..>3636)
                     /locus_tag="VFJ67_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7290407.1"
                     /translation="LKGAALWDEVHDRLNESALGMSGGQMQRLCIARAIAVEPEILLM
                     DEPCSALDPIATGKIEDLITELKSKYTIVIVTHNMQQASRVSDYTAFMYLGELIEYDT
                     TETIFLNPKYKQTEDYVSGKFG"
BASE COUNT          706 a         1017 c          921 g          992 t
ORIGIN      
        1 gatgagcttg atatcggcgc ccatcctcct caactcaccc gcgtggagga accgctgtgg
       61 gaatatggtc tcggtaagaa cgctgaggcc gtccgaggtg ctcatgagcg tcatcatctg
      121 cgcctgcata tcggtaggga atcccgggta gggcagtgtc gtgatgtcgg tgctcttgag
      181 cacgccgttc gacttcaccc tgatcccgcc gttctcgcgc gttatctgcg cccctatctc
      241 gatgagcttt cctatgagcg cccccatgtt ctcgaagcgg cagttcttta tcatcaagtc
      301 ccctttcgtg aggaccgaag ctatcatata cgtcccggct tctatcctgt cgggcattac
      361 ttcgtagctc tttagcggcg aaagcttctt tacgcccctt atcgtgatta tgtccgtacc
      421 gatgccggtg atgtcggcgc ccatctggtt gagcgctgtc gcgaggtctg taacctcggg
      481 ctcacacgcg gcgttcaaga gtatcgtttc cccttccgcg agcacggaag atagtatgag
      541 attctcggtc ccggtaacgg ttgaaacgtc gaaaggcact atcccgcccc tcagcctggg
      601 ggcgttcgtt tcgacgtagc cttcctctat cactatcttg gaacccagga tggaaagtcc
      661 tttcaggtgc tggtctatag gccgctcccc gatggcgcat ccgccgggaa gagatactct
      721 cgatttgccg aacctggctg tgagcgggcc gaggacgagg accgaggccc tcattgtctt
      781 gacgaggtca taaggggctt cccaactctg taaattggac gtatcgatcg agagagtgcc
      841 cctgtcgtat tcaacgtcgg ctcccagaat ctggaggagc ttgcccatgg ttctcacgtc
      901 cgcgaggtcg gggacgttcg taataatatt ctttccttcg gacaggatgc acgcagccat
      961 gagaggaagg acagcgttct ttgcgccgct gactacgatc tctccttcga gcctctttcc
     1021 gccctccact actatttttt ccattgagcc ttcacgagcc tctcgatatc gtttatgtct
     1081 cttacgcagg agatattatt gaaactggct tcttcaaaaa gctttatcac ttcgggcgac
     1141 tgtcccaccc ctatctcgag gaggcaccag ccgccggact tgagaaccct ttttgcgccc
     1201 gagattatct tccttatgaa gtaaagtccg tcctctccgc cgatgagcga ggagcggggc
     1261 tcgtaatccc tgacatcggg cgcgaggttt tcaaattccg cttccggtat gtatgggggg
     1321 ttcgatacca ggacatcgaa cgattcgtcc ctgaagggct cggccaggct tcccagaacg
     1381 aagaacattc cgctccccgc gccgtgcctc tcagcgttgg cccgcgcgag cgagagcgcg
     1441 cctccggaaa tatctgccgc aaagaccctc gaacccggct tttcgcagga tatcgtcacc
     1501 gctatgcagc cgctccccgt gcccacgtcg agtatcgcag ggtctccgat atccgctatc
     1561 gtccctaacg cttcctcgac cagaagctcg gtctcgggcc tcgggatgag aacgtctctg
     1621 ctgaccgaga aagccctcga gtaaaattcc ttttcgcccg tgatgtatgc gatgggttcg
     1681 ttcttgattc tcctttcgag gagcctgttg aattcatcta ccttgacctg gtcgagctcc
     1741 ttttcgggat gggcgtagat ttcggaaaca tcctcgatag cgctcgattt tgagagcagt
     1801 atggaggctt ctaacgcggg attctctatg aggtgaagcc tcatgttttt cttcccgagc
     1861 tggtagaggt ctcttaagtg catttcccga tgatccgcct tcagaaggct atgagtttat
     1921 acgtataacg catcttttca agaaaattat actatactac cggatacaaa tccttcccgg
     1981 ccggcggggg gagctctcac cggactcaag tctgaaatca ttcgtttgaa ataagaggta
     2041 attacagggg ggtgtaatgc gctttacgtc ttttcgagag agtgatgctt gagcgatttt
     2101 ccgcttacta tatagtaggt gtcctcggct atgtttgtag cgaggtcggc taccctttcg
     2161 atctccctcg ctatcagtat gatagtcagg gacctgtcta tggtctgagg gtcggcgatc
     2221 atgtaggtga cgagctctct cactatctgc ggctcgagcg cgtcgacttc gtcgtccctt
     2281 ttgcaaacgt ctatggcgag ctgggcgtct ttgttgacga aggcatcgag gctttcctga
     2341 agcatctgaa tagccttgtc ggccatcctg gggatgtcta taagtggctt tacgggcggc
     2401 ttgtcggcga gatatatcgt tttttccgct atgttgacgg cgtggtctcc gatcctctcg
     2461 agatcgttgt ttatcttcat aatcatggaa accgttcgca ggtcttccgc ctcggggtgg
     2521 tagagggcga gtatctttat gcactggttg tcgatctcga tctccatttc gttgatcttg
     2581 gcgtccgact tgattacctc ctcggccatg atcatattgc ggtcttttag agcttttatg
     2641 ctcttggcta tcatctcctc gacggaggtc gccatctcga acaacatttt cttaagcttg
     2701 cttatctctt cttctaatct gaccatattt ttatttccga acggggttcg gaatcctctt
     2761 ttcgagcgcc gcagctaata acgccgaact ccgactctat ccagtctttt atctcgtccc
     2821 tcactgtcct gaatccggcc agtatctctt cgtccctgcc cctgaacgcg gcgggatccc
     2881 tgaaccctct gtgtatatgc ctgccgccgc cggggaaaac ggggcaggct tcgttagcat
     2941 ggtcgcacac cgtgaccacg cagtcgaaac cagcaccctc gtacgcgtcc aagcttttag
     3001 acgagtgctc cgttatatcg attcctatct cgtccataac ccggacggca taggggttta
     3061 ctctcgacgg ccgggtcccg gcgctgtgca catcgaacct gtcgccgtac aagtgcctca
     3121 atattccttc cgccatttgc gaccgcgccg aattatgtgt acaaatgaat atcacagttt
     3181 ttttaccttc actaggcatc tgttgcttcc gataaaaata gttccggaga gataagccaa
     3241 ctgtcggatg aaggttcgaa attatccgaa tttcccggat acataatctt cggtctgctt
     3301 atatttagga tttaggaata tcgtttccgt cgtatcgtac tcaatcagct ccccaagata
     3361 catgaatgcg gtgtaatccg aaacacggga agcctgctgc atattatgag taacgatgac
     3421 tatggtgtat ttcgatttca actccgtaat gaggtcttct atctttcccg tggctatcgg
     3481 gtcgagcgcc gagcacggct cgtccataag cagtatctcc ggctctaccg ctattgccct
     3541 ggctatgcag agtctctgca tctgccctcc cgacatgccg agagcgcttt cgttgagcct
     3601 gtcgtggact tcatcccaga gggccgcgcc ttttag
//
LOCUS       DASSJH010000372         7959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1167618, whole genome shotgun sequence.
ACCESSION   DASSJH010000372 DASSJH010000000
VERSION     DASSJH010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167618"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFJ67_08230"
     CDS             <1..488
                     /locus_tag="VFJ67_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290408.1"
                     /translation="IHTEEAILITLMALSAIGIGVTNFKPVESFWYWAAMAPVFGAVS
                     IYIGWSKARRRGEGMSRIIWVQVLHWAGLLAAIALIYFLFRRTGRIDYNQLALISLLV
                     LALTTFLAGVHFDWRFMIVGIVLGACVAGAAFVEQVLWMIVIPIAAALVLFVFWWKRS
                     V"
     gene            730..1026
                     /locus_tag="VFJ67_08235"
     CDS             730..1026
                     /locus_tag="VFJ67_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7290409.1"
                     /translation="MAEKNPMTKSQLSNHIADMAGITKKTANEILDELAAIAYKEAKR
                     SKQFTIPGIGKLVLSKRKARIGRNPATGAEIKIPAKKVVKFRVAKACKDAVLGE"
     gene            1327..1704
                     /locus_tag="VFJ67_08240"
     CDS             1327..1704
                     /locus_tag="VFJ67_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7290410.1"
                     /translation="MPKVLLIEDEESLRNLYTRILSTKNYEVEAAADGEIALSLLKQF
                     RPDVIVLDIVMPHYNGVEILKILKNDGELKSIPVVMLTALSEMRKITECLDMGAVGYI
                     TKDSTVEEIVHRLNFLIESIGVQ"
     gene            complement(1688..2899)
                     /gene="coaBC"
                     /locus_tag="VFJ67_08245"
     CDS             complement(1688..2899)
                     /gene="coaBC"
                     /locus_tag="VFJ67_08245"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET7290411.1"
                     /translation="MSVIKGKKIILGVTGGIAAYKSCELVRSLVREGASVQVIMTQNA
                     MEFVTPLTLQTLSGNRVASKTFDLGWESEIGHISLADSADLIVVAPATASFIGKIASG
                     IADTLLTNVILATLSPVIICPAMNVNMYENRAVQENLETLRKRGFIIVEPSEGSLACG
                     WEGKGRLPETPDIVNEIEKTLTPQDFFGERVLVTAGATREYIDSVRFISNPSSGRMGY
                     ALAREGLLRGAEVVLISGKTSLTPPKGARVIDVVSAGDMYESVMEHLDWSTIVIKAAA
                     VGDYTPSEKINGKIKKGESGMILSLKRTKDILEAVGGRKNGRFVVGFAAETDNLLEHA
                     KEKLKRKTADLFVANDVSIPGAGFESETNLVHFVYRDGKTEELPGEAKTDIAGKIFDK
                     IRDMKPVIARR"
     gene            3055..3540
                     /locus_tag="VFJ67_08250"
     CDS             3055..3540
                     /locus_tag="VFJ67_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET7290412.1"
                     /translation="MPGRIKVEEKNITANAGLKDMAECRECTCFNLRKATRVVTQVYD
                     DAMRPTGLRATQFALLAHTYAMGPVPLSKLAEAMVTDRTTLARNLEPLEKEGLLSIED
                     GEDRRSRIISITEDGLKKFAGAYPVWKRTQDEVKKIMGEGEWSEMISKVSMMADRLRD
                     K"
     gene            3611..4966
                     /locus_tag="VFJ67_08255"
     CDS             3611..4966
                     /locus_tag="VFJ67_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7290413.1"
                     /translation="MSKMDEHPSVINYYKKRSLAGTAERGAGKSGVLSAAWLREVCRE
                     AGADDWGFVGIDSPDLSGDRDGILARFPRTRSLISIVCRMNREAIRTPARSVSNLEFH
                     HTGDRVNEVARRIVSTLEENGVWSLNPPMGFPMEMADFPGKIMVISHKTSAVAAGLGQ
                     MGINRNVIHPKFGNFMLLGTVLTDAEIDAYDKPVDYNPCVECKLCVAACPVGAIAPDG
                     YFNFSACYTHNYREFMGGFTSWVEGVAESGNAKEYRRRFTDSESASMWQSLSFGANYK
                     AAYCMAVCPAGEDVIGPFLESRKTYLDDVLKPLQNKEETVYVVRDSDAQTHVAKRFPN
                     KTAKTVSNGLRPKSVAGFLDGLPLIFQRDRSEGLDATYHFTFTGEEQLFATVIIRNKS
                     VEVHNELRGNPDLRVKADSRAWLGFIRKENGIIIPLLLKKIRLKGSIGLLKAFGRCFP
                     S"
     gene            5135..5632
                     /locus_tag="VFJ67_08260"
     CDS             5135..5632
                     /locus_tag="VFJ67_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7290414.1"
                     /translation="MNNKHRDGIESGKGLELASIMECSECTCFSLRKAARVVTQIFDN
                     ALRSIDLRATQFTLLALAFAHGPITVTKLSDEMVADRTTLSRNLNPMEKSGLIRITQG
                     NDKRTRIVVITDSGRRKLEEAYPLWKKAQMGIKEAMGTDKWRSLISNASDLVNLARLD
                     GGTSA"
     gene            5992..7569
                     /locus_tag="VFJ67_08265"
     CDS             5992..7569
                     /locus_tag="VFJ67_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7290415.1"
                     /translation="MKGYVRETPEGTGIKSVTESLVVGKNAGRWVLLVTILGSSMAFI
                     DSTAMNVVLPVLQMELDATIPQMQWIIEAYALFMSSLMLFGGALGDKFGRKRIFALGV
                     ILFTGSSLWCGLSGSTPQLIVARAFQGVGGALLVPGSLAIINISFSESDRGRAIGTWS
                     AFTAITTALGPILGGWLAENVSWRLVFFINLPIGIVVLATLYWRIQESRKDNGEGKLD
                     VLGSFLATMSLGCIVYALIESGNSGFGHPKVIVPFVAGGIALVSFLLYEAYAPSPMMP
                     LSLFKSRTFSGANFISVLFWAAWVGGVFFIPFNLIQLQGYSAAGVGIAFLPLVLALFL
                     FSRWAGGLVINYGAKPPLIIGTILAGVGFLLFTLPGIGGGYWTTFFPAIAVLGLGMAI
                     TISPLTTAVMSSIDLEHSGVASGINNSVGRIAGLLSVAILGVFALSGFNRNLDRELRS
                     LDLNEKTIRVIESQRIKLAAIDIPESLPEPTKKEIRGAISDSFLGAFRLMMKISAGLV
                     FLGAVTAWLTIEKNSPD"
     gene            7760..>7959
                     /locus_tag="VFJ67_08270"
     CDS             7760..>7959
                     /locus_tag="VFJ67_08270"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HET7290416.1"
                     /translation="MAEKMKKSDEDWKKELTEEQYYVTRQKGTERPFTGKLYENKEKG
                     MYKCICCGEELFSSDTKYESGTG"
BASE COUNT         1866 a         1934 c         2215 g         1944 t
ORIGIN      
        1 agatacatac ggaggaggcg atactcataa cgcttatggc gctatccgcg atcggtatag
       61 gagtgaccaa tttcaagccg gtcgagagtt tctggtactg ggctgcgatg gcgcccgtat
      121 tcggagccgt gagcatctac atcggatggt cgaaggccag gcggagaggg gaggggatgt
      181 cgaggatcat atgggtccag gtgctccact gggcggggct ccttgcggcc attgcactta
      241 tatatttttt gttcaggcgc acaggccgca tagattataa tcagctcgct ttgatttctc
      301 tgctagtgct cgcgctcacg acttttctcg ccggggtcca tttcgactgg cgcttcatga
      361 tcgtgggtat agtgctcggg gcatgcgtcg cgggcgctgc cttcgtcgag caggtcttgt
      421 ggatgatcgt tattcccata gcggccgcgc tcgtgctgtt cgtgttctgg tggaagcgtt
      481 ccgtctaaac tagttttttt tccgtcacct tgagaagctg taatggctcc cgggctcaat
      541 cggatgctat tggttaaagg ccgacctagt ccatctgctg tcacagctcc ccggcggatc
      601 agggatacgc cgcgatttcc tttgctcact gggttggcgg gcccttcata tttttcgtga
      661 aaatattgat tttcattgta cttttctgta ctattgatga cactaaaacc agttgtaaag
      721 gagagggaga tggccgagaa gaatcctatg acaaaatctc agttgtcaaa ccatatagcc
      781 gatatggcgg ggatcaccaa aaaaacagcg aacgagatat tggatgagtt ggctgcaatc
      841 gcttataagg aagcaaagag aagcaaacag ttcacgattc cgggaatcgg aaagctggtg
      901 ctgtccaagc gtaaggcgag aatcggaagg aatcctgcca ccggtgcgga gatcaagata
      961 ccggcgaaaa aagtcgtgaa gttcagggtg gccaaggcct gcaaagacgc tgtgttgggc
     1021 gaataacgct tatcactcaa tcttacagta gataatttaa atactaacca tccggagtcg
     1081 ttgcaaaatt caatgcgatt gtaacggatg tgccggaatg gtgcggtctt ttgcggggtt
     1141 tcggccctgc atactattga attaaataat cacacgtgaa tttttttggc tgtataattg
     1201 tcgggtagat ggcaatagtt cgttttaacc attgattttt accggtccaa gccgagctgg
     1261 tgcgtcggcc tcgcatcgct tgaacgcgaa ggccgatgca aatttttaga cgtgaggtcc
     1321 cgacaaatgc ccaaagttct attgatcgaa gacgaggaat ctttaaggaa cctctatacc
     1381 agaatactca gcaccaagaa ctatgaggtg gaagcggctg ccgacggaga gatcgctctt
     1441 tcgctgctga aacaattcag gcccgatgtg atagtgctcg acatcgtgat gcctcattac
     1501 aacggcgtgg agatccttaa aatactaaag aacgacggtg agcttaagag catacccgtc
     1561 gttatgctca ctgcgctttc cgaaatgcgc aagatcacgg aatgtctcga tatgggagca
     1621 gtaggatata taactaaaga cagtacggtc gaagagatag tgcacaggct gaattttttg
     1681 atcgagtcta tcggcgtgca ataactggct tcatgtcccg gattttatcg aagattttcc
     1741 ccgcaatatc cgttttggct tcaccgggca gctcctcggt ctttccgtcc cgataaacga
     1801 aatgaaccag attggtctcc gactcgaaac ccgctccggg tattgagacg tcgttggcga
     1861 cgaaaaggtc ggcggtcttt cttttcagct tttccttcgc gtgctcgagg aggttgtctg
     1921 tctccgcggc gaaacccacg acgaaccttc cgttctttct cccgccgacc gcctccagga
     1981 tgtcctttgt gcgtttgaga gatagtatca ttccactctc accctttttg atcttgccgt
     2041 ttatcttttc ggacggtgtg taatcgccta cggcggccgc cttgattact atggtcgacc
     2101 aatcgagatg ctccatgacc gactcataca tgtccccggc gctcacgacg tcgatgaccc
     2161 tcgccccctt ggggggcgtg aggctcgtct ttccggaaat aagaaccact tcggctccgc
     2221 gcaagaggcc ctctcttgcg agagcataac ccatcctgcc gctcgacggg ttagagataa
     2281 atctaacgga atctatgtat tctctcgttg cgccagctgt cacgagcacc ctctcgccga
     2341 aaaagtcctg gggcgttaat gtcttttcaa tctcgttcac tatatcggga gtctcgggaa
     2401 ggcgtccctt cccctcccat ccgcaggcca ggcttccctc ggaaggctcg acgattatga
     2461 aaccacgctt cctgagagtt tccaagttct cctggactgc ccggttttca tacatattta
     2521 cgttcatggc cggacaaatg attacaggcg aaagtgtggc caggattacg ttcgtaagga
     2581 gcgtgtcggc gatgccggaa gcgatcttgc ctataaaact ggctgtcgcg ggcgcgacca
     2641 cgatgaggtc ggcgctgtct gcaaggctga tgtgcccgat ttcggattcc cagccgagat
     2701 cgaaagtctt cgaagccacg cggttccccg agagggtctg cagagtgagg ggggtcacga
     2761 attccatggc gttctgggtc ataatcacct ggaccgaggc tccttccctg acgaggctcc
     2821 ttacgagctc gcaggacttg taggctgcta taccgcctgt cactcccagg attattttct
     2881 tgcctttgat tacggacatt atagtcaccc tttcattatt ttaccgaaaa aacctcactg
     2941 cccgaataat agtatattcc cctatagggg taagtaaaat ttttctcctg aaattaacag
     3001 ttgacacgta tatgataggt gtatatactc ctattgtgtg cgggagattg tttaatgcca
     3061 ggacgcatta aagtggaaga aaaaaatatt actgcgaatg caggcctgaa agacatggcc
     3121 gaatgccggg aatgcacctg ctttaacctc cgcaaggcga cgagggtggt gacccaggtc
     3181 tatgacgacg ctatgaggcc gactggactc agggctactc aattcgccct gctcgctcat
     3241 acgtacgcaa tggggcccgt acctttgagt aagctcgcgg aggcaatggt taccgaccgt
     3301 acgacactcg ccaggaacct ggaacctctg gagaaggagg gcctcctcag catagaagat
     3361 ggagaggatc gccgcagccg cattatcagc atcaccgaag atggactcaa gaaattcgcc
     3421 ggggcctatc ccgtatggaa gaggacgcag gatgaagtga agaagataat gggcgagggc
     3481 gagtggtcgg agatgatatc gaaagtgtct atgatggccg accgcctgcg agataaataa
     3541 aaaattttga tcatgttagt gtatatacac ctatatttca aagttaacgg tttacgaagg
     3601 agtaatgact atgtctaaga tggacgagca cccgtccgtg attaattatt ataaaaagcg
     3661 cagcctagcc ggcacagcgg aaaggggcgc gggaaagtcc ggcgttttaa gcgccgcatg
     3721 gctccgcgaa gtgtgccgcg aggcgggcgc cgacgactgg gggttcgtag gcatcgacag
     3781 tcccgatctt tcaggagata gagacggaat actcgcccgg tttccgcgta ctaggtccct
     3841 tatcagcatc gtttgcagaa tgaaccgcga agcgatacgc acccccgccc gctccgtttc
     3901 gaatctcgaa ttccaccata cgggcgacag ggttaatgag gtcgcacgca ggatagtctc
     3961 tactctggag gaaaacggcg tctggtcgct taacccgcct atgggttttc ctatggagat
     4021 ggctgatttt ccgggaaaga tcatggtgat atctcataag acgtccgcgg tagccgcggg
     4081 tctggggcag atggggataa accgtaatgt gattcatccg aagttcggga acttcatgct
     4141 cctgggaacg gtactgacgg atgctgaaat cgatgcgtat gacaagcctg tcgattacaa
     4201 cccctgcgta gagtgcaagc tgtgcgtcgc cgcatgtccg gtgggcgcaa tcgcgcccga
     4261 cggatacttc aacttttcgg cttgctatac gcacaattac cgggaattca tgggaggctt
     4321 cacaagctgg gtcgaaggtg ttgcggaaag cggtaacgcg aaggaataca gacgcaggtt
     4381 cacggattcg gaatccgctt caatgtggca gagcctgtcg ttcggagcta actataaggc
     4441 ggcctactgc atggctgtgt gcccggccgg agaggacgta atcggaccgt tccttgagag
     4501 ccggaaaacg tacctcgacg atgtcttaaa gcccttacag aataaggaag agactgttta
     4561 tgtcgtccgg gattcggatg cgcagacgca cgttgctaaa agatttccga acaagacggc
     4621 gaagaccgta tcgaacggtt tgcgtccgaa gtcggtagcc gggttcctcg acgggcttcc
     4681 gctgattttt cagagggata gatcggaagg tctggacgcg acctatcatt tcacgttcac
     4741 cggcgaggag cagttattcg caacggtgat aatacggaat aaatctgtcg aggttcataa
     4801 tgagctgaga gggaatcctg atcttagagt aaaggctgat agcagggcct ggctcggttt
     4861 tatcagaaag gagaacggta tcatcattcc gctgctcctc aaaaagatcc gtcttaaagg
     4921 gtctatcgga ttgctgaagg cgttcggaag gtgtttcccc tcctgagaat gaatcaccgt
     4981 aaaagtttgt cgctcttgct ccccgttaga ttatattatt aatcggcggt caagcggtaa
     5041 tgccggcagt gtggagatgt cttgctggtt tttctgcgtg ccatcgtgac tcggacgtac
     5101 aatctaaaat aattggacca tgacggaggg acttatgaac aacaagcaca gggatggtat
     5161 tgaatccggt aaagggcttg agcttgcctc tataatggaa tgcagtgagt gcacctgttt
     5221 tagcctgaga aaggcggcca gggtggtgac acagatattc gacaatgcgt tgaggtctat
     5281 cgacctgcgg gcgacacaat tcacgcttct ggccctagct tttgcgcacg gcccgataac
     5341 tgtaacgaag ctttcggacg agatggtcgc cgacaggacg actctctcac ggaatctcaa
     5401 tccaatggaa aagagcggtt tgataaggat tacgcaaggc aatgacaaga gaacgcggat
     5461 agtagtcatc acggattcag ggaggagaaa gctcgaagag gcgtatccgt tatggaagaa
     5521 ggcgcaaatg ggcataaagg aagcaatggg tacggataag tggagatctc tcatatcgaa
     5581 cgcttcggat ttggtgaatc tcgcgcgtct tgacggcgga acctccgcct gattgatgcc
     5641 aaattcaccg tattctccgt gctgagctat ctataattcc gtaagcatgc ttcggtcaat
     5701 gtatccggca ggagacccgg cgcgtgctat ttttgcgtcc ggaccgggtt cgccgcatat
     5761 gcgtccggac cggggtttat ggtgtaattc ccgttcaagc aggtagaata aagtcggttt
     5821 caggacttta ttttcgtacc ggcggatgcc cggcgggctt gcttgattcg catccgaccc
     5881 ggattcagga gcataagtct gatttcagga ttgcattatt tcgatacgtg cccgggaata
     5941 atattccagt tgatacgaac tcaataacaa gaacacctct ctctaatcat aatgaaagga
     6001 tatgtcagag aaacgcctga gggcaccggt ataaagagcg tgactgaaag tctcgttgta
     6061 ggtaaaaacg ccggaaggtg ggttcttctt gtaactatac tcggctcgag catggcattt
     6121 atagacagca ctgcaatgaa cgtcgtgctg ccagtgcttc agatggagct cgacgccacg
     6181 atcccgcaga tgcagtggat aatagaggca tacgctctct tcatgtcctc gctcatgctt
     6241 ttcgggggcg cgctcggaga caagttcggg aggaagcgta tctttgccct gggggtgata
     6301 cttttcacgg ggtcgtcgct ctggtgcggt ctctcgggaa gcacgcctca gctcatcgtc
     6361 gcaagggcgt tccagggggt aggcggggcg ctcctcgtcc cggggagtct cgccatcatt
     6421 aatatatcct tttcggagag cgaccgcggc agggccatcg gcacatggtc cgcgttcacc
     6481 gcgatcacta cggccctggg tcctatactc ggcggatggc ttgctgaaaa cgtttcctgg
     6541 aggctcgttt ttttcataaa cctgccgatc gggatcgtcg tgctcgccac tctttactgg
     6601 aggatacagg agagcaggaa ggacaacgga gagggaaagc tcgacgtcct gggctctttt
     6661 ctcgctacca tgagcctcgg gtgtatcgtt tacgcattga tagaatcggg gaactccggt
     6721 ttcggccacc ccaaggttat cgtgccgttc gtcgcgggcg gaatcgccct cgtctcattt
     6781 ctcctgtacg aggcgtatgc cccctcgccc atgatgcccc tcagtctgtt taaatccagg
     6841 accttcagcg gcgccaattt catttctgta ctgttctggg cggcctgggt gggtggtgta
     6901 ttcttcattc cgttcaatct gatccagctg caggggtatt cggcggcggg agtgggaata
     6961 gcgtttctgc ccctcgtact ggcgcttttc cttttttcga gatgggcggg gggtctcgtg
     7021 atcaactacg gcgcaaagcc gccgctaata atcgggacga ttcttgcggg tgtggggttt
     7081 ctgttgttca cactcccggg tatcggaggg ggatactgga cgacgttttt cccggcgata
     7141 gccgttctgg gcctgggcat ggcaataacc atttcgccgc tcactacggc cgtgatgagc
     7201 tcgatagacc tcgaacactc gggagtcgcc tcaggaatta ataattcagt gggcaggatc
     7261 gcgggccttc tttctgtcgc gatactcggt gtgttcgccc tctctggatt caacaggaat
     7321 ctcgacagag agctgaggtc gctcgacctg aacgagaaga cgatccgggt catcgaatca
     7381 cagcggatca agctcgcagc catagacata cctgaatcgc ttccggagcc cactaaaaaa
     7441 gagatacgcg gcgctatatc ggactccttt ctgggagcat tcaggctgat gatgaagatt
     7501 tcggccgggc tcgtcttcct cggagccgtc acagcctggc tcacgataga aaaaaatagt
     7561 cccgactgac gattctgtca cgatctggaa aaatactacg ccttcccgcg ataaattcat
     7621 tttctggttt tccccgtatc ataattgttc agattgtctg ttaatttagc ttaggttccg
     7681 gtttgataag atttccacgg gtcagtatcc tataaatatt gtcggtagat accagcgggt
     7741 atcaccgaag gagacgcata tggctgagaa aatgaaaaaa tcggatgagg attggaagaa
     7801 agagctcacc gaagagcagt attacgtgac gaggcagaag ggcacggaga ggccttttac
     7861 ggggaagctc tacgagaaca aggaaaaagg tatgtacaag tgcatctgct gcggcgagga
     7921 gctgttcagc tctgacacca aatatgagtc gggcaccgg
//
LOCUS       DASSJH010000373         4034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1274683, whole genome shotgun sequence.
ACCESSION   DASSJH010000373 DASSJH010000000
VERSION     DASSJH010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274683"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..439)
                     /locus_tag="VFJ67_08275"
     tRNA            complement(346..439)
                     /locus_tag="VFJ67_08275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(403..405),aa:Ser,seq:gct)
     gene            complement(896..1318)
                     /locus_tag="VFJ67_08280"
     CDS             complement(896..1318)
                     /locus_tag="VFJ67_08280"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET7290417.1"
                     /translation="MKVREVIKFLVENNGWYLVRICGSHRQFKLRSKKSRLTINCKMD
                     KELPEGTLFYSLKHCFDSQEVIHKEHYEITIEQSNDCYSAYCPDLPGCVAVGDTLEET
                     KELMLGSVRLHLEGLKQDGVLVSDPGNTSAFFEIRQAS"
     gene            complement(1365..1541)
                     /locus_tag="VFJ67_08285"
     CDS             complement(1365..1541)
                     /locus_tag="VFJ67_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HET7290418.1"
                     /translation="MHRGSLYIPAPLYERLRLFSFVSRKSQSKIIEDAIAEYLDNHMT
                     GKEFENVLMYRKDG"
     gene            1896..3038
                     /locus_tag="VFJ67_08290"
     CDS             1896..3038
                     /locus_tag="VFJ67_08290"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7290419.1"
                     /translation="MNFDGIDQFLGSRVDSGEIPGAAAAVVNRDGILYSGAFGLRDAA
                     NRVSMAPDDIFEIFSMTKPFTSVVVMMLLEEGKLGLDQPVSEFLSWLSKPEVIAGFDE
                     NTVSYETKPAQGEITVRHLLTHTAGYGYMFFNSEVNLMVKKTGRAATELPLLFEPGTR
                     WMYGPNTRVLGKAVEEITGMTLEEVLRERICGPLGLHDTCYALPEEKHGRLVTTHRRK
                     DGELVEDPRGEKPPLNMLGDTGLYSTAGDYATFLRLFLNGGKIDGTRLISEESVRMMV
                     SNQIGGLVIETMPGIMPELVNPFPTGGGRDKFGLGFQITVCEDNDALHRSHGSCSWAG
                     MKNTFFWFDLKIGIAAVLMMHVVPFYDEACINVYRGFEENVYKSLA"
     gene            complement(3066..3536)
                     /locus_tag="VFJ67_08295"
     CDS             complement(3066..3536)
                     /locus_tag="VFJ67_08295"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7290420.1"
                     /translation="MTKHGKDHPRVLAPPPFIAFVIILAGFAAGLIYPLRFIHGDARY
                     ISGGALLLLSAVIVSLAFSKMKGAGTNVDVRKPATTVVTGGIYSYTRNPMYVSLVIFL
                     AAVSVLLNNLWILIFIPVFVFIMQRGVIEREELYLEEKFGADYTDYKKRVRRWV"
     gene            complement(3529..>4034)
                     /locus_tag="VFJ67_08300"
     CDS             complement(3529..>4034)
                     /locus_tag="VFJ67_08300"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET7290421.1"
                     /translation="NVLLAGILIGAFSHTMLWRMEKGKRAFMFARSLPPAKRDMLLES
                     MMKMKNESRETRKKIRKARDEVIDAFSAPEFDPERFDSSVAALHALMRELTDEIAGET
                     KKIALELSPEERKQIADTMKRGPGPPFPRFLMEGSFGHDGPPPPPPPPPSDTDDWHDG
                     PGGDPHD"
BASE COUNT          933 a         1002 c         1115 g          984 t
ORIGIN      
        1 gcgattttct taacgcaaat tctgtaatgt cggaaaagat aaatcccccc tgcattccgt
       61 gccattcaga agaattccag ccccgaatta tttaacatca actgcgatga caagaaaaaa
      121 aacatccttt cactagctta aggctaatgc cggacaagca gggcgaacgg gtagggccgt
      181 gttgttgggt tgaactgatg agaaagtgta gtaccgaata cgagcgcagg ttctgcgctg
      241 tggcggagag cctccgcggg caaccgtgag ttatgttagt tcctgggatt tacccaacac
      301 tgaacgggtt ggaagaaatc atatacgagc acaggttctg tgctgtggcg gagagggagg
      361 gatttgaacc ctcgaagggg gtttaagccc ccttactcgc ttagcaggcg agcgccttcg
      421 accactcggc cacctctccg tgaacactgt ttatccggta attcctctac attattgaga
      481 acatataaag taaccaaagg gggggttagt gtcaacgaag ggaggggagg gtgtggagtg
      541 taatccgttc gtactgtgcc tgtcgaagta ttaatgggtt ttcttcactt cctgttatcc
      601 gctgatgtta catgagaatc atatatgagc gcagattctg cgcacttcga gatgcttagt
      661 cccaacaggt tttcttcgct gccggttaaa tacagaagat ttatgagaat tgtatatcag
      721 cacacattgt ccgcaaaaaa gggagcgcca gggatcgaat tcttcgcaga atcaatacct
      781 ggcgctctaa gggggggtat ggggttatgt cgttagttat gtttaatgct ttcttctcca
      841 tcatggcttt tcagccctga tgtcggcagc ccggaaagac gggaggctgc cgacatcagg
      901 aggcttgcct tatctcgaaa aaggccgatg tgtttcccgg atccgaaacc agaacgccgt
      961 cctgcttgag gccttcaagg tggaggcgga ccgagccgag cattagctcc tttgtctctt
     1021 cgagagtatc tccgacggcg acgcagccgg ggaggtccgg gcaataagcc gaataacagt
     1081 cattcgactg ctctatcgtg atctcgtagt gttctttatg aattacttcc tgggaatcga
     1141 aacaatgctt gagagagtaa aagagggtgc cttccggcag ttccttgtcc atcttgcagt
     1201 tgatggtgag cctcgacttc ttgctcctca gcttgaactg gcggtgactt ccgcaaattc
     1261 tgacgagata ccatccgttg ttttcaacaa ggaacttaat cacctctctc actttcataa
     1321 tctccatcct ctcacagggt attttgtaaa gccgtaaatt ccgtctatcc gtctttgcgg
     1381 tacatcagca cgttttcgaa ctccttgccc gtcatgtggt tgtcgaggta ctcggctatg
     1441 gcgtcctcga ttatcttcga ctggcttttt ctggagacga atgagaagag cctgagtctc
     1501 tcataaagcg gtgcggggat atagagggag cctctgtgca tcttctccgg atttgcccgc
     1561 tgctgaattg ttgccgccgt catgatctca ttattacatc attacgtaat gatgtcaata
     1621 tggtattttg ttaagctgaa aaaatattca ggcgcggaat attcttatgt ggttacaagt
     1681 gcttatggcg ggcactacgg cccggactaa atcgcgcgcg agttctttat cgtatacaac
     1741 agggataggt tctcgggaac gaaccgattg tatgtaccgg agtcccgtga aaggctataa
     1801 ttcatatcgt aaaataaggc aaatgcgccg attgttgcta gagtcctcac catgtccgca
     1861 agccttcggg aataaaactc aggagagctt acgttatgaa ttttgacggg atcgatcaat
     1921 tcctcgggag cagagtggat tccggtgaaa taccgggagc ggccgctgcc gtggtgaaca
     1981 gggacgggat actgtatagc ggcgctttcg gactcaggga cgcggctaac cgtgtctcga
     2041 tggcgcccga tgacatattc gaaattttct cgatgacaaa gccgttcacg tcggtcgtcg
     2101 tgatgatgct gcttgaagaa ggcaaactcg gcctggatca gcccgtatcg gaattcctgt
     2161 cctggctctc gaaacccgag gtaatagccg gtttcgatga aaacaccgtt tcatacgaga
     2221 caaagccagc tcaaggggag ataacggtaa ggcatctgct cacgcatacc gcggggtacg
     2281 ggtacatgtt ctttaacagc gaagtgaacc tcatggtcaa gaagaccggg agagccgcga
     2341 ccgagctccc tcttcttttc gagccgggca cgagatggat gtacggtccc aacaccagag
     2401 tcctcggcaa agcggttgaa gagataacgg gaatgacgct cgaggaggtc ttgcgtgaga
     2461 ggatatgcgg tccgctcggc cttcacgata cgtgctatgc gcttccggag gagaaacacg
     2521 gcaggcttgt taccacgcat cgaagaaagg acggggagct tgtggaagac ccgcgcggtg
     2581 aaaagccccc gttgaacatg ctcggggata cgggcctcta ttcgacggcg ggggattacg
     2641 cgacgttcct ccggctgttt cttaacggcg gaaagattga cggcacgaga ttaatcagcg
     2701 aagagtcggt gcgaatgatg gtaagcaacc agataggcgg gctcgtaatc gagacgatgc
     2761 ccggtataat gccggagctc gtgaatccct ttccgaccgg aggagggagg gacaagttcg
     2821 gtctcggatt ccagatcacc gtgtgtgaag ataatgacgc gcttcaccgc tcacacggca
     2881 gctgcagctg ggccgggatg aaaaatacgt ttttctggtt cgatcttaaa atcgggattg
     2941 ccgctgtatt aatgatgcac gtagtgccgt tttacgacga ggcttgcatc aatgtttaca
     3001 gaggctttga agaaaacgtt tacaagtccc tcgcctgaga ccgctccaca gcggcgtgta
     3061 ggaatctata cccaccttcg cacgcgtttc ttgtagtccg tgtaatccgc gccgaacttc
     3121 tcctcgagat agagctcttc cctttcgatc acgcctctct gcatgataaa tacgaagacc
     3181 ggtatgaaga tcaagatcca gaggttattg agcagtacgg ataccgcggc gagaaagatg
     3241 accagggaca cgtacatggg attcctcgta tatgagtaga tgccgcctgt gacgacagtc
     3301 gttgcgggct tcctcacgtc gacgttcgta cccgcgccct tcattttgga aaacgccagt
     3361 gacactatca ctgcggataa gagcaggagc gctccgcccg atatgtatct cgcatctccg
     3421 tgtatgaaac ggagagggta tataagccct gccgcgaagc ccgcgagtat aatcacgaac
     3481 gcgataaacg gcgggggagc aagcaccctc ggatggtcct tgccgtgttt agtcatgcgg
     3541 atctccgcca ggcccgtcgt gccagtcatc cgtgtcggaa ggcggcggag gcgggggcgg
     3601 cggggggccg tcgtgtccga aagacccttc cataagaaag cgcgggaacg gggggccggg
     3661 ccctctcttc atggtgtccg ctatctgctt tctttcttcc gggctcagct caagggctat
     3721 cttcttcgtt tctccggcta tttcgtccgt aagctctctc atgagcgcgt gcagcgcggc
     3781 cacgctactg tcgaagcgct cagggtcgaa ttcgggagcg gaaaacgcgt ctatgacctc
     3841 gtccctcgcc tttctgatct tcttcctggt ctcgcgggac tcgttcttca tcttcatcat
     3901 cgattcaagg agcatatcgc gtttggcggg cggcaggctt ctcgcgaaca tgaacgcccg
     3961 tttccctttc tccatgcgcc atagcatcgt gtgcgaaaac gctccgataa gtattccggc
     4021 gaggagaacg ttaa
//
LOCUS       DASSJH010000374         5084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1390015, whole genome shotgun sequence.
ACCESSION   DASSJH010000374 DASSJH010000000
VERSION     DASSJH010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390015"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFJ67_08305"
     CDS             <1..470
                     /locus_tag="VFJ67_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7290422.1"
                     /translation="EWGECFLTGDEVGFLRRLPLDRDFTIDGIDFEIAHGSDPYSYIF
                     YNMVIGSGEQHEDLVVHPENGEKRRFILAGHSHKPFVKNIGKTTVLNPGSVGQPRDRD
                     PRASYGVIENGEASIRRVSYDIGKTVKDLERSGMPYPVKARLISMLVSASVIN"
     gene            complement(733..2127)
                     /locus_tag="VFJ67_08310"
     CDS             complement(733..2127)
                     /locus_tag="VFJ67_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET7290423.1"
                     /translation="MKAIFKLLLIAVLVLPLSGVAAKAESANQQIEELKEQIRQLQIQ
                     NQQQIQQLQQQVQTLQNAQQQSDQTLSQINKKQVEEEDAWYKKFLAKYDNGFTFESTD
                     KEGLPFKMKFSILAQIQGSVTDTDTKDVATNFRLRRLELKWSGIAFAPWFYYTFMIDP
                     ASSSLLKDMYFTAQYQNPIGARIGQWKVPFQKEELNSSAALQFVERSIVNAEFTLERE
                     PGLALQGALGAHNNFSYAAGVFNGDGINGSSVDSNMLYAGRIQLGLGGDDDKFDANGT
                     YATAKSYNLVPNFAKKPTFVIGAAASALPGLDCSVKTPNGNVCNRITALSFGNDVDFT
                     QIEGDAMFKMQWFNVEAEYDGRWLNSSAAGLGTAYDQGFRAQAGVFLMPKTFELAGRY
                     ALIDYDTGSDVVPADTSEPDKTWEVTPGLNYYMSHDHRWKIQLTYTFQRQEFTEGAPD
                     IDSNIFRAQVQANF"
     gene            2300..2989
                     /locus_tag="VFJ67_08315"
     CDS             2300..2989
                     /locus_tag="VFJ67_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7290424.1"
                     /translation="MTEKLITIVDDEEDIVKLVSHHLKREGFKVKEFHNGRDFLSYIE
                     SVTPDLAVLDIMLPGIDGLEICRILKNKSTMASVPIIMLTAKAAEADVVVGLELGADD
                     YIVKPFSPRELVARVKTVLRRTSAKESDDNIIKIGPLTINTEKYEVAVDNDKVLLTTT
                     EFKILEVLAEGKGRVFTRDQLLKKKRLWGDDKLVYDRTIDVHIKNLREKLGTAGNMIK
                     TVRSIGYKLEE"
     gene            2997..4766
                     /locus_tag="VFJ67_08320"
     CDS             2997..4766
                     /locus_tag="VFJ67_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7290425.1"
                     /translation="MSIFWKQFISSFLIIFLILSLFTFLVIGELQNYDKSLTKERLLT
                     AATLAGDILEPSIDKGNRPEIQRLVYALGGKTGVRITVIDGSGLVLGDSSRNPAEMEN
                     HADRPEIKDAIANDFGESDRYSETLRKEMLYVAVPYRGHGNRILAVIRTSLPITTLEK
                     TIHPMETKVIYLGVILTLVALLLSLGLSKTISHSLGGIIRSSEELAKGNLDISIPVTG
                     GKSEISKISMALNQMAKKLSELFKQLSREKNQLQAVLSAMSEGVMVVSREGRVIIINS
                     ALKKMFNLKEDPTQKPYWEILRNRELTKLVESVLINWMPDSREIFYLYPDEKHYFANV
                     IPLDSPDKEVIVVMFDITDFKRLEKIKADFIANVSHELRTPLTAIKGYTETLEEEAYE
                     SPEDRKHFLKIIKRHTDRLINIVSDLLVLSEVESRDSLSRENTANDLEETNINELIRS
                     SLDALRSKAQEKGIEVSYRSDNDAYKIKANRFLLEQMFINLIDNAVKYTPENGKVDVR
                     VAKEDSHILTEISDTGIGIPKEHLPRIFERFYRVDKTRSRNLGGTGLGLSIVKHIVIM
                     HGGRIEVQSEEGRGSKFSIYLPA"
     gene            complement(4827..>5084)
                     /locus_tag="VFJ67_08325"
     CDS             complement(4827..>5084)
                     /locus_tag="VFJ67_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET7290426.1"
                     /translation="TSIVFSLRDKPGELQKSFFQPFAEARVNLTKIESRPSKERPWEY
                     LFFVDFVGHREEKHIRNLLDKVAKNCVSMKVLGSYPIGRNV"
BASE COUNT         1344 a         1267 c         1352 g         1121 t
ORIGIN      
        1 gggaatgggg ggagtgcttc ctgacgggag atgaagtggg attcctgaga aggctcccgc
       61 tcgacaggga tttcacgata gacgggatcg atttcgagat agctcacggc tcggatccgt
      121 acagctacat attttataat atggttatag ggagcggcga gcagcacgag gatctcgttg
      181 tccacccgga aaacggtgag aaacggagat ttattctggc cggacattcg cataagccat
      241 tcgtaaagaa catcgggaaa acgactgtcc tgaatccagg ctccgtaggg cagcccagag
      301 accgcgaccc gagagcatcg tacggcgtaa tcgagaacgg cgaggcctcg ataagaaggg
      361 taagctacga tataggaaag acggtcaagg acctggaaag aagcggcatg ccgtacccgg
      421 tgaaggcgag gctcatatcc atgctggtct ccgcctcggt catcaactga atttaccccc
      481 cagccataac cgattacgtc ccgtctgcgc ccaaagcggc agacaggccg ccctcgtctt
      541 gtttcctcct gattactgaa aaattgacga ctacggaacc cggaccgtag ggtttcgtct
      601 atccgggtta ataaaaaaga agcccggcac atgaggccgt ctcatgtacc gggcttcgga
      661 gctttcggag ccagaatgaa aaaagggaga gatgagattc ttttttcttc tttctacgac
      721 agcacagccg aattagaagt tggcttgtac ctgagccctg aatatatttg aatcgatgtc
      781 gggcgcgcct tcggtgaact cctgtctctg gaacgtgtat gtgagctgga tcttccacct
      841 gtggtcatgc gacatgtaat agtttaatcc aggcgtaact tcccacgttt tgtcgggttc
      901 gctcgtatcc gcaggaacta catccgaacc ggtatcatag tctatcaatg catatcttcc
      961 cgctaactca aacgtcttcg gcataaggaa tacgcccgcc tgcgccctga agccctggtc
     1021 atacgcggta cccaaaccgg cggcgctgga attaagccac ctgccgtcat attcggcctc
     1081 tacgttgaac cactgcatct taaacatggc atcaccctct atctgcgtga agtcaacgtc
     1141 atttccgaaa ctcaacgcag ttatcctgtt gcagacgttg ccgttggggg ttttcacact
     1201 gcaatcgaga ccgggtaaag ccgatgcggc cgcgccgatg acgaatgtgg gttttttggc
     1261 aaagttggga acgaggttgt aagacttcgc cgtcgcgtat gtgccgttgg cgtcgaactt
     1321 atcgtcgtct ccgcccaggc cgagctgtat cctgccggcg tagagcatgt tggaatcgac
     1381 gcttgatccg tttataccgt ctccattaaa cacgccagct gcgtatgaaa agttattatg
     1441 tgccccaaga gcgccctgga gtgcgagacc gggctctctc tcgagtgtaa actcggcgtt
     1501 aacaatagat ctttcgacga actggagggc ggctgaagaa ttaagctctt ccttctggaa
     1561 aggaaccttc cattgtccga tcctggcacc tatcggattc tgatactgag cggtgaagta
     1621 catatccttc aagagcgagc tgctggccgg gtctatcatg aacgtataat agaaccatgg
     1681 ggcaaatgct atgcccgacc acttaagctc gagtcttctc agacgaaagt ttgtcgccac
     1741 atccttcgtg tcggtatcgg tgacagagcc ctgaatctgg gcaagtatgc tgaatttcat
     1801 cttgaacggc aaaccctctt tatcggtcga ctcgaaggtg aaaccgttgt catacttggc
     1861 aaggaacttt ttataccacg catcctcctc ttcaacctgt ttcttgttaa tctgggaaag
     1921 cgtctgatcc gactgctgct gtgcattctg gagtgtctgc acctgctgct gtaactgctg
     1981 tatctgctgc tggttttgaa tctgtaactg ccgtatctgt tcctttaatt cctcgatctg
     2041 ctgatttgcc gattccgcct ttgccgcgac acccgaaagc ggcagaacca gaacggcgat
     2101 caacagcagc ttgaatatag ctttcatcac tcgttctcct cctgtaaaag aatttgggga
     2161 acatattaga gagaaataat taatctgatg tgaaattaat gttaagatac tgttaagggg
     2221 ggcgtatgtt attgcaagta gccgcttatc gtttaatcta aggggtagtt tcctgtatgc
     2281 ttagaatagg ttaggtgcta tgacagaaaa actgatcacc atagtagacg atgaagaaga
     2341 tattgttaag ctcgtaagcc accacctgaa gagggaaggg ttcaaggtga aggagttcca
     2401 caacggcagg gatttcctct cgtatataga atccgtcaca ccggaccttg cggtgctgga
     2461 tataatgctg ccgggaatag acgggctcga gatctgcaga atcctgaaaa acaaatcgac
     2521 aatggcttct gtgcccatta tcatgctgac cgccaaggcc gctgaggcgg acgtggtagt
     2581 ggggttagag ctcggagcgg acgattacat agtaaaaccg ttcagccccc gcgagctcgt
     2641 agccagggta aagacggtgc tcaggagaac aagcgccaag gaatcggacg acaacatcat
     2701 caaaataggg ccgcttacga taaacaccga gaagtacgag gttgcggtcg ataacgataa
     2761 ggtgttactc acgacgacgg agttcaagat cctggaagtt cttgccgagg ggaaaggacg
     2821 ggtatttaca agggaccagc ttctgaaaaa gaagcggctt tggggggacg acaagctcgt
     2881 ttatgacaga acgatcgacg tacacatcaa aaacctcagg gaaaaactcg ggaccgccgg
     2941 caacatgata aaaacggtaa gaagcatcgg ttataaacta gaagagtagg atcctgttga
     3001 gcatcttttg gaaacagttc atttccagct ttctgataat attcctcata ttgtccctgt
     3061 tcactttcct cgttatcggc gagctgcaga attacgataa atcgctgacc aaggaaaggc
     3121 ttttgacggc ggcaaccctc gcgggcgata tactcgagcc ttccatcgac aaggggaacc
     3181 ggcccgagat acagcgtctt gtatacgcgc tcgggggaaa gaccggggtc aggatcacgg
     3241 tcattgacgg aagcggcctg gtgctggggg actccagcag aaaccccgcg gaaatggaaa
     3301 accatgcgga caggcccgaa ataaaggacg ctatcgcgaa cgacttcggt gaaagcgacc
     3361 gctacagtga gacgctccgg aaggagatgc tctatgtggc ggttccctac agggggcatg
     3421 ggaaccggat attggcggtt atacgcacgt cgcttccgat taccaccctt gagaaaacga
     3481 ttcacccgat ggagacgaaa gtcatctacc tgggggttat ccttacgctc gtcgcccttc
     3541 tcctctcgct cgggctctca aaaaccataa gccactccct aggagggata ataaggtcgt
     3601 ccgaagagct cgcaaagggg aacctcgaca tcagcatccc cgtaaccggc ggaaaatcgg
     3661 aaatctccaa aatctcgatg gcgctcaatc agatggcgaa aaaattaagc gagctgttca
     3721 aacagctgtc acgggaaaag aaccagctcc aggccgtgct cagcgccatg agcgaaggag
     3781 taatggtcgt atcgcgcgag ggaagggtca taatcatcaa cagcgcgctt aagaagatgt
     3841 tcaacctgaa agaggacccg acgcagaaac cctactggga gattctgaga aacagggagc
     3901 tgacgaagct cgtcgagagc gttctcatta actggatgcc cgactcgagg gagatattct
     3961 atctgtaccc ggacgaaaaa cactacttcg cgaacgtgat ccctctcgat tccccggaca
     4021 aggaggtaat cgtggtaatg ttcgacatca cggatttcaa gaggctcgag aagataaaag
     4081 ccgatttcat cgcgaacgtc tctcacgagc tgagaacccc tcttaccgct atcaagggat
     4141 atacggagac gctcgaggag gaagcctacg aaagcccaga ggaccggaag cactttttga
     4201 aaataataaa gcggcacaca gacagactga taaatatcgt gtccgacctg ctcgtgctct
     4261 cggaagtcga aagcagggac tccctctcca gggagaacac cgctaacgac ctcgaagaga
     4321 ctaacatcaa cgaattgata aggtcctcgc tcgacgcgct caggtcgaaa gctcaggaaa
     4381 aggggataga ggtatcgtac cgctccgata acgacgccta caagatcaag gcaaacaggt
     4441 ttctcctcga gcagatgttc atcaacctca tagacaacgc agtaaagtac actcctgaaa
     4501 acggaaaagt cgacgtcagg gtcgcgaagg aggattccca tatactgacc gagatcagcg
     4561 atacgggcat aggcataccg aaggaacacc tcccccggat attcgaacgg ttttacaggg
     4621 tggacaagac acgctcaagg aacctgggcg gcactggact cgggctcagc atcgtcaagc
     4681 acatcgtcat aatgcacggc ggcaggatag aggtccagag cgaagaggga aggggaagta
     4741 aattcagcat ctatctgcct gcctgatttc cttaaccgca agtattgcca agtattgccg
     4801 gccgggtcct ccaggaaacg gggggatcac acattccggc cgatagggta ggaacccagc
     4861 accttcatac tgacgcagtt tttagctacc ttgtcgagaa ggtttcttat atgtttttct
     4921 tccctgtgtc cgacgaagtc gacaaagaag agatactccc acggcctttc cttggaaggg
     4981 cgggactcga tcttggtgag attgacccgg gcctcggcaa agggctggaa aaacgatttc
     5041 tggagctcgc ccggtttatc cctgagggag aaaacgatcg atgt
//
LOCUS       DASSJH010000375         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1275171, whole genome shotgun sequence.
ACCESSION   DASSJH010000375 DASSJH010000000
VERSION     DASSJH010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275171"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..1739)
                     /locus_tag="VFJ67_08330"
     CDS             complement(330..1739)
                     /locus_tag="VFJ67_08330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7290427.1"
                     /translation="MSENPKVAIVIVTWNKKSYVMELLQSLRGIDYDNHDIIVVDNAS
                     TDDTCRAIREQFPQVNLIVNSENLGGTGGFNTGLRYVLKKGGYKYTWLLDNDAVVERD
                     TLIELVKVMEEDDKVGITGSIIMEPDLDMIVELGAYVSWSIGTWKPNYRNNRLSAIKE
                     IDTTEVDYVAACSSLVRNEALYKIGVMDERYFIHWDDIDLSLRAKEAGYKVMASPKSK
                     AYHGLEDKPINPLISYYDIRNGLLTITKHQNGLRRFLYILNMLRGTGKGAVYSLLSGL
                     NTTSKLLVYGPLDFIRGKFYKSSLSVKTEDDPDPASMVIPFERLNIRNSSKVIILPNA
                     SYTVIKNMVARIRELSPDCHVSLLIQEDRYELFKDLEIDRFIVFEGYRQSTIQTLSVL
                     KEILESNYDLCISPSRSMLPFSYAVKKYYLYDHKRDYFVKSKEDIYASWKLFVSFFGG
                     ELLAIVLMPFVYLKSLAQQ"
     gene            complement(1799..>2094)
                     /locus_tag="VFJ67_08335"
     CDS             complement(1799..>2094)
                     /locus_tag="VFJ67_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7290428.1"
                     /translation="DFSEIEQKFMDMPVKKYSNGMNARLGFSIAIHSSADIILIDEIL
                     AVGDESFQAKCISKIKEIKGYNKTVVIVSHNKKKMEELTDRILYIKSGEIINS"
BASE COUNT          464 a          514 c          488 g          628 t
ORIGIN      
        1 agactgcatc caggtttttt tgtttagcct tcctcaagcc cgaaaagtcc agatcaacta
       61 gtattattct cagtccggga tattcttcgg tcagtttgcg ggaggtgaat ccgtcatagg
      121 atccgatatc gagaacagtt gaataattct ttatttccga tacgagtatt cttttcaatg
      181 catgccatct tagtaaagtt ccaggtctca tcgataaata aatttaatgc tcttttggaa
      241 atgatacggc tttggatgaa aattcggttt tctcatatgg tcggattttg attttctcac
      301 aaaatctaaa ggagtctgtg cggcatcccc tactgctgcg ccaggctctt caaatagaca
      361 aagggcatga ggactattgc gagcagctcg cccccgaaga acgagacgaa cagcttccag
      421 gaagcgtaaa tgtcctcttt gcttttaacg aagtagtccc tcttgtgatc gtagagatag
      481 tattttttga ctgcgtaaga gaagggcagc atcgagcggc tcgggcttat gcacaggtcg
      541 tagttggatt cgaggatttc tttcaatacg gagagcgtct ggattgtcga ttgcctgtat
      601 ccctcgaaca cgataaaacg gtcgatttcg aggtctttga aaagctcgta tctgtcctcc
      661 tgtatcagga gcgagacatg gcagtccggg ctcaattcac gtattctggc gaccatgttt
      721 ttgatcacgg tgtagctcgc attcggaagg attatcactt tcgagctgtt tcttatattc
      781 aggcgctcga aaggaatgac catggacgcg gggtcggggt catcttcggt tttaaccgac
      841 agactggatt tatagaactt gccgcggatg aagtcgagcg gtccgtaaac cagcagcttc
      901 gaggtagtat tcaagccgct tagtaaggaa tatacggccc cttttccggt gccgcgcagc
      961 atattcagaa tgtataaaaa cctcctgaga ccgttctggt gcttcgtgat tgtgagcagc
     1021 ccgttcctga tatcgtaata ggatatgaga gggtttatgg gtttgtcctc gagcccgtga
     1081 tacgcctttg acttggggga ggccataacc ttataccccg cttccttcgc cctgagtgac
     1141 aggtctatat cgtcccagtg tatgaaatac ctctcgtcca taacgccgat tttatataga
     1201 gcctcgttcc tgacgagaga cgagcaggcg gcgacgtaat cgacctccgt cgtgtctatc
     1261 tccttgatgg cgctcagcct gttattcctg tagttgggtt tccacgtccc tatcgaccac
     1321 gacacgtaag ccccgagctc gacgatcatg tccagatccg gctccatgat gattgatccc
     1381 gtgatcccga ccttgtcgtc ctcctccatc acctttacga gctctatgag cgtatccctc
     1441 tctactacgg cgtcgttatc gaggagccac gtgtacttgt aacccccttt cttgagcacg
     1501 tatctgaggc ccgtgttaaa tccccctgtc ccgccgaggt tctcggaatt cactattagg
     1561 tttacttgag ggaactgctc ccggatcgca cggcacgtat cgtcggttga agcattatcg
     1621 actactatga tatcgtggtt atcgtagtcg atgccgcgga gcgactgaag gagctccatg
     1681 acatagctct ttttattcca tgtcactata actattgcaa ctttcgggtt ttcactcatt
     1741 ccggttcttt ctgtaaaaat gcctggtttg atttctatta tagcctaaaa tccgcgcttt
     1801 acgaattaat tatctcgccg ctctttatgt ataaaatcct gtcggtaagc tcttccatct
     1861 ttttcttgtt gtgagagact atcaccacgg tcttgttgta ccccttgatc tcttttatct
     1921 tgcttatgca cttcgcctgg aacgattcgt ctccaacggc cagaatctcg tctatgagta
     1981 ttatgtcggc gctgctgtgg atcgcgatcg agaatccgag cctggcgttc atgccgttcg
     2041 agtatttctt taccggcatg tccatgaact tttgctctat ctccgagaaa tcga
//
LOCUS       DASSJH010000376         5475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1603393, whole genome shotgun sequence.
ACCESSION   DASSJH010000376 DASSJH010000000
VERSION     DASSJH010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603393"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..827
                     /locus_tag="VFJ67_08340"
     CDS             93..827
                     /locus_tag="VFJ67_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7290429.1"
                     /translation="MSDKVALITGGARGIGREIALALAEREWSVAVCYRKSRKEAAET
                     VKEIERRGGRGAHHKCDVSNPSSAVELVRKVEEEWGRIDALINGAGPYHRVNLLKESV
                     EGWHEMFDNNLHPVFYLSRTVAEGMKSRGWGRIISFGMANADKMSAQTEITAHYIAKA
                     GVLILTRSLARLLAPHGITVNAISPGFIYSGSAPMEELESMVKTIPAGYIGSTKDAVD
                     ASLYLLSDEARYVNGTNIHLSGGWGL"
     gene            complement(891..3752)
                     /locus_tag="VFJ67_08345"
     CDS             complement(891..3752)
                     /locus_tag="VFJ67_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HET7290430.1"
                     /translation="MSRFISLITVILFSLPVSSMAGISLTPVGTYGAGVFDDGAAEIV
                     GYDPASESVFVINANASVVDVLSIADPANPTLSFSIEVSADLLANEGFGSGGVNSVDV
                     KNGIVAVAAENTDPALNGYLVFYDTAGTYLGFVNVGVLPDAVKFSPDGKKLVVANEGE
                     PVGAVNPPGSVSVVDISGGAAAAVSDTLGFTAFDGREAEFLNKGLKLVNGVPMSLDLE
                     PEFPAISPDGTQAFVTLQEANAFAVVDLLTPEIVDILPLGVKDHSTGQPELDLFKFDD
                     LPLIGIDANKLKIKPGGFSGLHYDGSGKKGVLRFLTVSDRGPNGADVVNGSRTFNLPG
                     YQARVIAFEVNKKNNNGHHGHGRTNNGGGHDDNGEAEITKTILLTRADGKTPITGLPN
                     IPGFDEIPVDAKGKPVKYDRFGADLEGIVRDPADGSLWMVDEYRPSIYHFTDKGRMIV
                     RLVPENTHLLGDSAQKQAQFGPHANDPGFYGMETLPEVYSKRRSNRGFEAVALDPDKR
                     LLYAFIQSPMENPNTSTRSSHVLRILAVDVDESSPDYLNPVAEYVYLLERPVHTPDDV
                     DKIGDAVFIGDDRFLVLERDSALDPTSKKFVFEIDLKGATDIRNNPIADEMSSGTLEQ
                     ETADSLAALGIRAVFKRKVLNLPSLGYLPSDKIEGVTMLPDGRIAVLNDNDFGIEASQ
                     GFKPVLGLITFSAGNMLDASDKDSAINIANWPVLGLYQADGIDKYTYNGKTYYVTANE
                     GDTRDVEEVRVKSLSLDPDVFPDAASLKLDANLGRLKATNVIGDIDGDGDHDRIFSFG
                     ARSFSIWDSFGNLVFDSSHDFENITADALPAQFNSDNTDNTSFDERSDDKGPEPEGVA
                     VGKVGFKVYAFIGLERISGIMIYDITKPASPKFVDYVNNRNFNVDAQNPDDSSNPLAG
                     DLGPEGLFFVPSSDSPNGKALLIVGNEISGTTTVYQIDN"
     gene            3948..4985
                     /locus_tag="VFJ67_08350"
     CDS             3948..4985
                     /locus_tag="VFJ67_08350"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290431.1"
                     /translation="MDLELLKKLCEAPGLPGHEGPVKEIVRGIFPEFTSEISEDALGN
                     IIAHVPGKGPRLVIDAHTDEVGFMVSHVDGKGFLHVIPLGGMDPRVFYAQRIVVWGRR
                     PLVGTVAAIPPHVSRNSNNKEVPEVEDCVVDLGLSAEKVKELVKVGDVASFYYPFEET
                     EDAIISKAIDDRVGLFIMIEALRKKPELSCDLYLTASCQEEVGLRGAHVIVPAIEPDF
                     AIAIEGTVAMDVPGVSDHKTLANTCKGPEVRLSDRFLVAHRPFSDFIMKTAEQKKIPY
                     QITVKKAGSTNATAMQVTGKGTRAASVSVPVRYLHSPSCLAYKSDIAHTIDLITAVIE
                     NIGDFGKDSAR"
     gene            5003..>5475
                     /locus_tag="VFJ67_08355"
     CDS             5003..>5475
                     /locus_tag="VFJ67_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290432.1"
                     /translation="MRNEKLSTHVRALIFLVILFVCGNAYPESASLDTSVPARTVPTL
                     DDAWKFFGGYNQNYKRIDETVAMTEEILKRDPDNVPALIFLSRVWLTYGYVKARTRED
                     LIRVFENGKEAAKKAIEIDPRNADAHFFYVANLASLGDTKGLFNSLFMLPEVRREL"
BASE COUNT         1171 a         1520 c         1597 g         1187 t
ORIGIN      
        1 tacggttcat tattcaccgg aaatatcgtg tggcaatgat acccgctttg cactattatt
       61 tgattaatta cagcaccggg aggtctgtga atatgtccga taaagtcgcg ctgataacgg
      121 gaggggccag ggggatagga agggaaatag cgcttgcact cgccgagagg gagtggtctg
      181 tcgccgtctg ttacaggaag agcagaaagg aagccgccga gacagtgaag gagatcgaaa
      241 ggcgcggcgg gaggggcgct caccacaaat gcgacgtatc gaacccatcg tcggccgtcg
      301 agctcgtgcg caaggtggaa gaggaatggg gcaggatcga tgcgctgata aacggcgcgg
      361 ggccctatca ccgcgtcaac ctgctcaagg aatccgtcga gggctggcac gagatgttcg
      421 acaacaacct ccacccggtc ttctatctct cgcgcaccgt cgcggagggg atgaaatcgc
      481 gcggctgggg gaggataatc agcttcggca tggctaacgc agacaagatg agcgcacaga
      541 ccgagataac ggcgcattac atcgccaagg cgggcgtgct cattctcacg cgctctctcg
      601 cgcggctcct cgcgcctcac ggcatcacgg tgaacgccat ctcacccgga ttcatatatt
      661 cggggagcgc gccgatggag gagctcgaaa gcatggtgaa gactataccg gccggctaca
      721 tagggagcac gaaggacgcc gtagacgcgt cgctctatct tttgtccgac gaagcgcggt
      781 acgtgaacgg gacgaacata cacctgagcg gcggatgggg attgtaaacg gataaaaaaa
      841 aggggggacc atgaccggct ccccctctca ctcgtttact caaaactctc tcagttgtct
      901 atctgataaa cagtagtcgt gccgcttatt tcattcccga ctatgagcag ggctttgccg
      961 ttcgggctat cgctcgacgg gacgaagaac agcccctccg ggccgagatc acccgcaaga
     1021 gggttcgagc tgtcgtccgg gttctgtgca tctacattaa agttgcggtt gttcacgtaa
     1081 tcgacgaatt tgggcgatgc cggcttagtg atatcgtaga tcattattcc gcttatcctc
     1141 tcgagtccga tgaaagcgta aaccttaaac cctacctttc ccacagcgac gccctctggc
     1201 tcgggaccct tgtcgtcgct cctctcgtcg aaggacgtgt tgtcggtatt gtccgaattg
     1261 aactgcgcgg ggagagcgtc tgccgtgatg ttctcgaagt cgtggctcga atcgaatacg
     1321 aggttgccga aggagtccca tatcgagaat gacctcgctc cgaagctgaa tatcctgtcg
     1381 tggtctccgt ccccgtcgat gtcccctatc acgttggtcg ccttgagcct gccgagatta
     1441 gcatccagtt tcaggctcgc tgcgtccggg aatacgtccg ggtcgagact gaggctcttg
     1501 acccttacct cttccacgtc cctcgtatcc ccctcgttcg ctgttacata gtacgtcttc
     1561 ccgttatacg tgtacttgtc tatgccgtcc gcctgatata atccgaggac gggccagttg
     1621 gcgatgttta tagcggagtc cttgtcggag gcgtcaagca tgttgcccgc cgagaacgta
     1681 ataaggccga ggacgggttt gaatccctga gacgcctcta tgccgaagtc gttatcgttg
     1741 agcacggcga tgcgcccgtc tgggagcatc gttactccct caatcttgtc gctgggcaga
     1801 tacccgaggg agggcaggtt gagcaccttc ctcttgaaaa cggctcttat cccgagcgcg
     1861 gcgaggctgt cggccgtctc ttgctcgagc gtgccgctcg acatctcgtc ggctatcgga
     1921 ttgtttctta tgtctgtcgc gcctttgagg tctatctcga aaacgaattt cttgctcgtc
     1981 gggtcgagcg cgctgtccct ctcgagaacg agaaacctat cgtcgccgat aaagacggcg
     2041 tcccctatct tatcgacgtc gtcgggcgta tgcaccggcc tttcaaggag atatacgtac
     2101 tcggcgaccg ggttcaggta atcaggggag ctctcgtcca cgtccactgc gagtatcctg
     2161 agcacgtgtg agctccgggt cgatgtattc gggttctcca tcggagactg tatgaacgcg
     2221 tagaggagcc tcttgtcggg atcgagagcg accgcctcga acccgcggtt agatctccgc
     2281 ttgctgtaaa cctctggcag cgtctccatg ccgtagaacc ccgggtcgtt tgcgtgcggg
     2341 ccgaactgcg cctgcttctg cgcggagtct ccgaggaggt gcgtgttttc gggcacaagc
     2401 ctgactatca tcctaccctt gtccgtgaag tgatagatcg aaggcctgta ctcgtccacc
     2461 atccagagcg agccgtcagc cgggtccctg actatgcctt cgaggtcggc gccgaagcgg
     2521 tcgtacttga caggcttgcc cttggcgtcg accggtatct cgtcgaagcc gggaatgttc
     2581 ggcaaccctg ttatgggggt ctttccgtcg gcgcgcgtga ggaggatagt cttcgttatc
     2641 tcggcctctc cgttatcgtc gtgtcctccg ccgttattgg tcctcccgtg accgtggtgg
     2701 ccgttgttat tttttttatt gacctcgaac gcgataacgc gcgcctggta tccggggagg
     2761 ttgaaggtcc tggagccgtt tacgacgtca gcgccgttgg gtcccctgtc tgagaccgtg
     2821 agaaatctga gtacgccttt cttgcctgag ccgtcgtagt ggagtcccga gaaaccgccc
     2881 ggtttaatct tcagtttatt ggcgtcgatt cctataaggg gcaggtcgtc gaatttgaag
     2941 aggtcgagct cgggctgccc ggtgctgtgg tccttcaccc cgagaggcaa aatatcgact
     3001 atctcgggcg taagcagatc gaccacggcg aacgcgtttg cctcctggag agtgacgaat
     3061 gcctgcgtgc cgtcagggga gatagcgggg aactcgggct cgaggtcgag agacatcggc
     3121 acgccgttaa ctaacttgag gcccttgtta aggaactcgg cctccctgcc gtcaaacgcc
     3181 gtaaaaccga gcgtgtcaga gacggccgcg gccgcgccgc ccgatatgtc gacgacgctg
     3241 accgagcccg gagggtttac cgcgcccaca ggctcgccct cgttcgctac gacgagcttc
     3301 ttgccgtcgg gcgagaactt gaccgcgtcc ggaagaaccc ctacattcac gaatcccagg
     3361 tacgtacctg cggtatcgta gaatacgagg tagccgttca aggcagggtc cgtgttctca
     3421 gctgcgactg cgactattcc gttcttgacg tcaacgctgt tcactccgcc gctcccgaag
     3481 ccttcgttgg ccaggagatc tgcggatact tcgatcgaga acgatagcgt cgggtttgcc
     3541 ggatccgcga tgctgagcac atcgacgacc gacgcgttcg cgtttatcac gaataccgat
     3601 tccgaagctg ggtcgtagcc tactatctcg gcggctccgt catcgaacac gcctgcaccg
     3661 tatgttccga ccggtgtcag gcttatgccg gccatcgacg aaaccggaag cgaaaacagt
     3721 atgaccgtta tcagagaaat gaatctcgac attattactt cctcctttaa ttttttttat
     3781 taattgaatc gagaagctgg agagagtttc ggcgattttt ataaagccgg ggttaaccgg
     3841 aggttaaacg aaggttaaga acgcggcgag tgaaacggaa cggttacagc cggccgggat
     3901 cctgtaaact ggaattcttt tcacgctttg ttataatcat ctacccaatg gacctggaat
     3961 tacttaagaa actctgcgaa gcacccggtc tccccggaca cgaaggccct gtaaaggaaa
     4021 tcgtgagagg gatattcccg gaattcacta gcgagatttc ggaggacgcc ctcggcaata
     4081 taatcgcgca cgtacccgga aaggggccga ggctcgtcat agacgcgcac acggacgagg
     4141 tggggttcat ggttagtcac gtggacggga agggttttct ccacgtcatc ccgctcggcg
     4201 gaatggaccc gagggtattc tacgcgcaga ggatagtcgt ctggggacgg aggccgctcg
     4261 tgggtactgt agccgcgata ccgccccacg tgagcaggaa ctcgaacaac aaggaagtgc
     4321 ccgaggtgga ggactgcgtc gtggatttag gcctttcggc tgaaaaggtt aaggagctgg
     4381 ttaaggtcgg ggacgtcgcg tcattctatt acccattcga ggagacggag gacgcgataa
     4441 tatcgaaggc gatagacgac agggtcggcc tcttcattat gatagaagcg ctcaggaaaa
     4501 aacctgaatt atcgtgcgac ctctatctca cggcttcctg ccaggaggag gtggggttga
     4561 ggggagcgca cgtgatagtg cccgcgatag agcccgactt tgccatagcc atcgaaggca
     4621 cggtcgctat ggacgtgccg ggcgtatccg accacaagac gctagccaac acctgcaagg
     4681 gccccgaagt caggctctcg gacaggttcc tggtcgccca caggcctttc agcgacttta
     4741 taatgaaaac ggcggagcaa aagaaaatcc cttaccagat aacggtgaag aaagcgggct
     4801 ccactaacgc cacggccatg caggtgacgg gaaagggcac gcgcgcggcg tccgtttcag
     4861 tccccgtcag gtacctccac agcccgagct gcctcgccta taaatcggac atcgcccaca
     4921 ctatagacct cataaccgcc gtcatagaga acatcgggga ttttgggaag gactccgccc
     4981 gctgatatac ttattccgga ttatgcggaa tgagaaatta tccactcacg tgcgggcgct
     5041 gatattcctt gtaatactgt tcgtttgcgg aaacgcttac ccggagagcg cctctctcga
     5101 taccagcgtg cccgcaagaa ccgttccgac gctcgacgac gcctggaagt tcttcggcgg
     5161 ctacaatcaa aactacaaaa ggatagacga gaccgtcgcc atgacggagg agatattgaa
     5221 acgggacccg gacaacgtcc cggccctgat attcctctcc cgcgtctggc tcacctacgg
     5281 atacgtgaag gcgcggacca gggaggacct catacgcgtc ttcgagaacg ggaaggaagc
     5341 ggcaaaaaag gccatagaga tagacccccg gaacgcagac gcccattttt tctacgtcgc
     5401 caacctggca tccctcggcg atacgaaggg actattcaat tcgctcttca tgctgcccga
     5461 ggtgaggagg gagct
//
LOCUS       DASSJH010000377         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_949869, whole genome shotgun sequence.
ACCESSION   DASSJH010000377 DASSJH010000000
VERSION     DASSJH010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949869"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1386
                     /locus_tag="VFJ67_08360"
     CDS             163..1386
                     /locus_tag="VFJ67_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7290433.1"
                     /translation="MGKLSSSHTGTGCRVLASVLLIVCLSSPIVGTDQLIPEASAQKS
                     EAVFFELNDHGVRTGDESGLNEYLTMLKNDPGLVIVIDGYSDITGEEQYNLNLSRMRA
                     DAVKKFYVSHGIAADRIKSRGNGKTDKFDSGIDDESLKMNRRVVITLESAPVKKNSPP
                     VKPVPSPAAADAPEATPLPESHEEVIEEGAAIEDAPQSVPSLEPRATAAPELPAPPPD
                     LEKAIRRSMRNIAPGRVVFDAPAVMNVGETYDVEVDLSYSFLKGLSESLRGMSGEGFD
                     KVRLGQNVGLSLNGRGFVIRPVTEAGGGEEVFPAEDTGITKLVTENSNPRWNWKVTPL
                     ASGYKSLQLSVEIMAEDADFNETVNKYPLFQKVVDVKPNFIYMLSRSYWIMVVFIIVV
                     VAVVGWILIKKIRLG"
     gene            1414..1851
                     /locus_tag="VFJ67_08365"
     CDS             1414..1851
                     /locus_tag="VFJ67_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7290434.1"
                     /translation="MKWLRNILRGKMPVRGIDHIAIVVSDMDRSLEFYTATLGMELIK
                     DGRPEGGLKKSFVGTGGKTLLALTEDKNRSGAAADRVEGVNHIAFYVDDMDRAGGSLR
                     EKGVRIIEEKVSQDGRITACHFLDPDGLELEICTQTGKVTPQY"
     gene            2063..>2780
                     /locus_tag="VFJ67_08370"
     CDS             2063..>2780
                     /locus_tag="VFJ67_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7290435.1"
                     /translation="MRVLIVGGGISGLTLAGLLRQRGFRPTLVERAPEYGSVGYIIVL
                     WPSGSRILKGLGLYRKLLEAGMPFSTYNVWNEKGEMLRSYSVESVTEKYGPMISIYRP
                     ELIDILREAVDPGTIRMNTTVENIRQTPDEVFVTFSDGREEVFDLVVGCDGVRSKTRS
                     MVFGHVPLTYSGMKGWSFWVKSGIQKTAHIAEYWGTGKFLGVWPTKGRLSVFTSIRSP
                     EGSSGKVENGVIRIGEHFKDF"
BASE COUNT          657 a          662 c          853 g          608 t
ORIGIN      
        1 gggcggcggg gctcccgccg ggattgagtg agttttcgga gtcccgggcg gcgcttgaaa
       61 tctggtccac ctccttattg acaacggatt gattaaagtg agataatggt aattatcata
      121 ttagctgctc ctattccata actggtccag ccgaggcgga ggatgggtaa attgtctagc
      181 tcgcataccg gaacgggatg ccgcgttctc gcttccgttc tgctgattgt atgcctttct
      241 tccccgatag tgggcacgga tcagcttata cccgaagcat cggcgcagaa atcagaagcg
      301 gtcttttttg aacttaacga tcacggcgtc agaaccggcg atgagtccgg tctcaacgag
      361 tatctgacta tgctgaagaa cgaccccggt cttgtgatcg tcatcgacgg ctactcggac
      421 atcacgggcg aagagcagta taatctcaac ctgtcgcgaa tgagggccga cgcagtaaag
      481 aagttttatg tgagtcacgg gatagctgct gacaggataa agtccagggg caacgggaag
      541 acggataaat tcgacagtgg aatagacgac gaatccctca agatgaacag gagggttgtg
      601 attactctcg aatccgctcc cgtgaagaag aatagtcctc cggtaaagcc cgttccttct
      661 ccagcggccg cggatgcgcc tgaggcaacg cctctgcccg agagccacga agaagtaatc
      721 gaggagggag cggcgattga ggacgcgccg cagtctgtgc cttcgttgga gccccgggcg
      781 accgccgcac ccgagctgcc ggcgcctccg cctgacctgg aaaaggcgat acgccgctcc
      841 atgaggaata tcgcgcccgg acgggtggtg ttcgatgccc ccgcggttat gaacgttgga
      901 gagacttacg acgtcgaggt cgacctttcg tattctttcc tgaagggtct ctccgagagc
      961 cttcgcggca tgagcggaga ggggttcgat aaagtgaggc tcgggcagaa cgtcggcctc
     1021 agcctgaacg gacgggggtt cgtcatacgc ccggttactg aagcaggggg cggcgaggag
     1081 gtgttcccag ccgaggatac gggtattacg aaactcgtta ccgagaattc gaatccacgc
     1141 tggaactgga aggtcacccc tctcgcgagc ggctacaagt cgctgcaatt gtcggtcgaa
     1201 ataatggccg aggacgccga tttcaacgaa acggtgaata agtaccctct tttccaaaaa
     1261 gtcgtcgatg ttaagccgaa ttttatttac atgctttcga ggagctactg gataatggtc
     1321 gtttttatca ttgtagtagt ggcggtcgtg ggctggatat tgataaagaa aataaggctg
     1381 ggctgaggtc gtgagcagga ggggatacaa ctcatgaagt ggctgcgtaa catcttacgt
     1441 gggaagatgc ccgtgagggg tatcgatcat atagctatcg ttgtgagcga tatggacagg
     1501 tctctcgaat tttataccgc taccctgggt atggaactga taaaagacgg acggccggaa
     1561 ggcggactca aaaaatcatt cgtcggaacc ggagggaaaa cccttttggc tctgacggag
     1621 gacaagaaca ggtcgggtgc tgccgcggac cgggtggaag gcgtcaacca catcgccttt
     1681 tacgttgatg acatggacag agcgggcggg tcactcaggg agaaaggagt acggatcatt
     1741 gaagaaaaag tcagtcagga cggtaggatc accgcctgcc acttcctcga tccggacggg
     1801 ctcgagctcg agatttgtac ccagacgggc aaggtgacgc cccagtactg agccggctga
     1861 agtcagattt ttgatgtgtg catattcgtt tgtttgcgcc gggttaacta tatttatatg
     1921 tggtatacta agagtctgtg gttctgaggc gctttatctt aaccatgaaa attttcatct
     1981 attaagttga cgataaataa tgcggaaaat taaatcgtct gattcgggta aatataatct
     2041 tcctgtccgg tacgggcccg agatgcgcgt acttattgtc gggggcggca tatccggact
     2101 tacgcttgcg ggtcttctcc gtcagcgcgg gttcaggccc actctcgtag agcgcgctcc
     2161 cgagtatggg agtgtgggat atataatagt tctctggccg tcgggcagcc ggatactcaa
     2221 gggactcggc ctttacagga agctcctcga agcgggaatg ccgttttcca cgtacaacgt
     2281 gtggaacgaa aagggggaga tgctgcgctc gtattccgtc gagagcgtga ccgaaaagta
     2341 cgggcctatg ataagcatat acaggccgga gctcatcgat atactcagag aggccgtcga
     2401 ccccgggacc atacgaatga acaccacagt ggagaatatc cgtcagacgc ctgacgaggt
     2461 gttcgtaacg ttcagcgacg ggcgggagga ggtattcgac ctggtcgtag gctgtgacgg
     2521 ggttcgttcc aagacgcgca gcatggtgtt cggacacgtg cccctcactt acagcgggat
     2581 gaaggggtgg agcttctggg tcaaatcggg aattcagaaa acggctcata tagccgagta
     2641 ctgggggaca ggaaagttcc tgggcgtctg gccgaccaag ggccgcctct ccgtattcac
     2701 gagcatcagg agcccggaag gatcgtccgg taaggtagag aacggagtga tccggatcgg
     2761 ggagcacttc aaggacttcg
//
LOCUS       DASSJH010000378         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1499073, whole genome shotgun sequence.
ACCESSION   DASSJH010000378 DASSJH010000000
VERSION     DASSJH010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499073"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..539)
                     /locus_tag="VFJ67_08375"
     CDS             complement(93..539)
                     /locus_tag="VFJ67_08375"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7290436.1"
                     /translation="MQIRTGLLIYSIGHSNRTLDELVSTLKRYGVETLADIRTFPRSK
                     RNPHFDRESLEKELPASGIGYVWIRELGGMREDGYEHYMSTTGFEEGFNALLAVAGER
                     RTAFMCSELDWRNCHRSFVSHALHSRGWEIVHIYDENESETHSGLF"
     gene            982..1986
                     /gene="trpS"
                     /locus_tag="VFJ67_08380"
     CDS             982..1986
                     /gene="trpS"
                     /locus_tag="VFJ67_08380"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7290437.1"
                     /translation="MAKGKPILVTGDRPTGRLHLGHLVGSLVNRIKFQDDYDCYFLVA
                     DLHMLTTHYDKVGEVERNTIEMVMDWLSVGMNPSKSTFYIQSQIPYIAELYAILAMFC
                     GVPRAERIPTLKEKVQDMGVGENYSVGLLGYPVLMAADILMFKAEKVPVGEDQLSHVE
                     LTRELARRFNFLYGEFFKEPEPVISETSRLVGTDGNRKMSKSLDNCIYLSDSPKEVEA
                     RVRSMYTDPKRIRADIPGTVEGNPVFVYHDAFNTDTEEVEDLKQRYRTGNVGDVEVKK
                     KLSRAINELLEPIRERRSEFEKRPDYVKDVLREGTRRANEAAHINMDEIITKMGLYKP
                     "
BASE COUNT          494 a          588 c          557 g          426 t
ORIGIN      
        1 agataccgag caagtcggtg agcaggcaca tggcggattt cgtctgcggt tacgacccgg
       61 acgcggcgaa gaaggcgacc cctcccgcga aatcaaaata ggcccgagtg ggtctcggac
      121 tcgttctcgt cgtatatgtg cacgatctcc caaccgcgtg agtggagcgc gtgcgagacg
      181 aagctcctgt ggcagttcct ccagtcgagc tccgagcaca tgaaagcggt ccttctctcc
      241 ccggcgacgg ccaggagagc attgaacccc tcctcgaacc ccgtcgtgct catgtaatgc
      301 tcgtatccgt cctcgcgcat ccctccgagc tctcttatcc atacgtatcc gatgccgctt
      361 gcgggcagct ccttttcgag cgactccctg tcgaagtgcg ggttccgctt cgagcgcgga
      421 aacgttctga tatcggcgag cgtctcgacg ccgtaccgct tgagcgtcga tacgagctcg
      481 tcgagagtcc tgttcgaatg gcctatggaa tatataagca gtccggttct tatctgcacg
      541 atagaatagt atgttcgccc gtcgtccccg gcgcaacccg ccgaccatat ttttcttaag
      601 ttctgtcatt cccgaaacga ttgcgagcaa cagttctgag caagcgtaag aactggtcgc
      661 tgccggctga ttaccagcgt gtaactatac ttggcaagag gcacatcaat acaaattggc
      721 aatcggtcac tacaggctga ttgctaagat gtctattatc taggaaactt acttaaataa
      781 actaaatctt tatcgggaat ccatttcttt cgcctataat tccctcctta gaaaaaggag
      841 ggatagggag gattttgcat ttgtcttttc gaaccgaaga tgagaaatcc gtctccaaac
      901 cactatctgt cccattttcc cccgaattgt gtatattaat cccccaaacc ggccggataa
      961 agcataagga gtatcggatt aatggcgaag ggtaaaccta ttctcgtgac gggggacagg
     1021 cccaccggga ggctccacct ggggcatctc gtaggctccc tcgtgaaccg cataaagttc
     1081 caggacgact acgactgcta tttcctcgta gccgacctcc acatgctcac gacccactac
     1141 gacaaagtag gcgaggtcga gcgcaacacg atagagatgg tgatggactg gctctcggtc
     1201 ggcatgaacc cctcgaaatc cacgttctac attcagtcgc agatacccta catcgccgag
     1261 ctctatgcca tactcgccat gttctgcgga gtcccgcggg cggagagaat tcccacgctc
     1321 aaggaaaagg tgcaggacat gggcgtcggc gagaactatt cggtcggact cctcggatac
     1381 ccggtcctca tggcggccga tattctcatg ttcaaggccg aaaaggtccc cgtgggagag
     1441 gaccagctca gccacgtaga gctcacgcgc gagctcgcga ggcggttcaa cttcctctac
     1501 ggcgagttct tcaaggagcc cgagcccgtc ataagcgaga cgtcgcgcct cgtcggcacc
     1561 gacggcaaca gaaagatgtc gaagagcctg gataactgta tctacctctc cgacagcccg
     1621 aaagaagtgg aggcccgcgt gaggagcatg tacacggacc ccaagcgcat acgcgccgac
     1681 atccccggca cggtcgaggg caacccagta ttcgtatatc acgacgcgtt caacaccgat
     1741 accgaagagg tcgaagacct gaagcagagg tacaggaccg ggaacgtggg cgacgtcgag
     1801 gtgaagaaga agctctcacg cgcgataaac gagctgctag agccgattag ggaaaggagg
     1861 agcgagttcg agaagaggcc cgattacgtg aaggacgtgc tcagggaagg cacgcgccgg
     1921 gctaacgaag ccgcccatat caacatggac gaaataatca cgaagatggg gctttataag
     1981 ccctgaccgc cggaccggtt agctttgtat agaatcctgg gaggattcct ggtcatcccc
     2041 gtctgattcc tcactgccct tatcc
//
LOCUS       DASSJH010000379         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_626281, whole genome shotgun sequence.
ACCESSION   DASSJH010000379 DASSJH010000000
VERSION     DASSJH010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626281"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2403)
                     /gene="topA"
                     /locus_tag="VFJ67_08385"
     CDS             complement(124..2403)
                     /gene="topA"
                     /locus_tag="VFJ67_08385"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET7290438.1"
                     /translation="MAKSLVIVESPAKAKTIKKFLGKSFAVEASSGHLVDLPSSKLGV
                     DIENDFNPHYVVIKGKSKYLNQLKKAAKDADKVYLASDPDREGEAIAWHIADKLNIRD
                     KSHRVLFHEITENAVKESIAHPTTLSQDRFEAQQARRILDRLVGYQVSPILWRKVKKG
                     LSAGRVQSVALRLVVDREREIEAFKSEEYWTIEADLKRPEDAQEASFTAVLSTYDGEK
                     VEISDGARANSIVDSVRNEKFTVNSVERKERKKNALPPFITSTLQQEASRKLRFSMKK
                     TMTLAQKLYEGIDLGEEGPVGLITYMRTDSVRISENAIREAREIIKETYGDEYLPQKP
                     NSFRVKKSAQDAHEAIRPTYAGKHPDSVKQYLPDDELQLYKLIWQRFIASQMNPIIYD
                     QTSVDIAAGRGVFRATGSVVKFPGFSAVYLEGMEEEEEEKEKGEMRKLPDLTKGDGLD
                     LLGIEGIQHFTQPPPRFTESSLVKELEEKGVGRPSTYATILSTIIDREYVSKEKSKLK
                     PTLLGCSVNDLLVQGFPEIMDVKFTADMEEKLDNVEDGNVNWVELLKGFYSDFSLRLD
                     KAQETMKSLKRDGIPTDISCDKCGAPMIIKWGKNGEFLSCSRYPECKNAKAFEYDPEG
                     KVKVVEKVPPVIREDIKCDKCGASMVIKRSRRGEFLACTRYPDCKNAKTFEYDPDGNI
                     KIIEKAEPVIREDVKCEKCGKPMAVRSGRFGKFLGCTGYPKCRNIKGIDENGNPVDPK
                     QNGKGQGKKKSGGDQAVLN"
BASE COUNT          464 a          775 c          669 g          716 t
ORIGIN      
        1 gcctattttt atctgtctcg aattcctctg catacttttc actgcattta attatacgtg
       61 atttatttta cgcgacacac aggaagcatc aataaaaaac ggacagtagt tcaaacgccg
      121 ggatcagttg aggacagcct ggtctccgcc gcttttcttt ttgccctgcc ctttcccgtt
      181 ctgcttgggg tcgaccgggt tgccgttctc gtcgatgccc tttatgttcc tgcacttcgg
      241 gtatccggtg cagccgagga acttgccgaa ccttccgctc ctgacggcca tcggctttcc
      301 gcatttctca cacttgacgt cttcccttat gacgggctcc gctttctcga ttatcttgat
      361 attaccgtcc gggtcgtact cgaacgtctt ggcgttcttg cagtcggggt agcgcgtgca
      421 cgcgaggaat tccccgcgcc tgctccgctt tatcaccata gaagcgccgc acttgtcgca
      481 tttgatgtct tcccttatca caggcgggac cttctcgacg acctttacct tgccctcggg
      541 atcgtattcg aatgccttgg cgttcttgca ttcggggtaa cgggagcacg acaggaactc
      601 tccgttcttc ccccatttta ttatcatggg agcgccgcat ttatcgcagg atatgtctgt
      661 gggaatgccg tccctcttga ggctcttcat cgtttcctga gccttgtcga gccgtaacga
      721 gaagtcgctg tagaacccct tcaggagctc cacccagttg acgttcccat cctccacgtt
      781 gtcgagcttc tcctccatgt cggccgtgaa cttcacgtcc atgatctcgg ggaaaccctg
      841 tacgagcagg tcgttcaccg agcatccgag gagcgtcggc ttgagcttgc tcttctcttt
      901 ggagacgtat tcgcggtcta ttatcgtcga gagtatcgtc gcataggtgg agggacgccc
      961 gactcctttt tcctcgagct ccttcacgag cgagctttcg gtgaacctgg gcgggggctg
     1021 tgtgaaatgc tgtatgccct cgatgcctaa gaggtcgagc ccgtcgccct tcgtgaggtc
     1081 ggggagcttt ctcatctcgc ccttctcctt ctcctcctcc tcttcctcca tgccctcgag
     1141 gtaaacggcc gagaacccgg ggaacttcac gacagagccg gtcgccctga agacgccccg
     1201 gccggcagct atatccacgc tcgtctggtc gtatataatc gggttcatct gggaggctat
     1261 gaacctctgc catatgagct tatagagctg gagctcgtcg tcggggaggt actgttttac
     1321 cgagtccgga tgcttgcccg cgtatgtggg ccttatggcc tcgtgcgcgt cctgcgcaga
     1381 cttcttgacc ctgaacgaat tcggtttctg cggcaggtat tcatcgccgt acgtttcctt
     1441 tatgatttct ctcgcttccc tgatcgcgtt ctcggatatc ctgacggagt ccgtcctcat
     1501 gtaagtgatg agccccacgg gaccctcttc gccgagatcg atgccttcgt agagcttctg
     1561 cgcgagagtc atggtttttt tcatggagaa cctgagcttc cgtgacgcct cctgctggag
     1621 cgtgcttgtg atgaaaggcg ggagagcgtt ctttttccgc tccttccttt cgacggagtt
     1681 gaccgtgaac ttttcgttcc tcacggaatc gactatggag ttcgcgcggg cgccgtccga
     1741 tatttcgacc ttttccccgt cgtaggtgga gaggacggcg gtgaaagagg cctcctgcgc
     1801 gtcctcgggt cttttgaggt cggcttcgat cgtccagtat tcctcggact tgaacgcctc
     1861 gatctcgcgc tccctgtcga ctacgagcct gagagcgacg ctctggaccc ttccggcgct
     1921 gagacccttc ttgaccttcc tccacaggat cgggctcacc tgatagccga cgaggcggtc
     1981 gagtatcctt ctcgcttgct gtgcctcgaa cctgtcctga ctcagcgtgg tcggatgggc
     2041 tatcgattcc ttgactgcat tctcggttat ttcatgaaac agcacgcggt gggatttgtc
     2101 ccttatgttg agcttgtcgg cgatgtgcca cgctatcgcc tcgccttccc tgtcggggtc
     2161 cgacgcgagg taaaccttat cggcgtcctt ggccgccttc ttgagctggt tcagatactt
     2221 gctctttccc ttgatgacca cgtaatgcgg attgaaatcg ttctctatgt cgacgcccag
     2281 cttgctgctc ggcaggtcga cgaggtgacc cgatgaggct tcgacggcga agctcttccc
     2341 caggaatttc tttatcgtct tcgccttggc gggagactcc acaattacaa gagacttagc
     2401 cattcaacct cctttccctc gttaagctgt ttaccacaat attatgcata tgcaaaaccg
     2461 aatcaatgcc cggcccgaaa aacaaaggca ttttacctga atatatttct atgcaaatat
     2521 cggccctccg atccgccgtc gctgaaaatc aggcccggag acgcgaagcc gctccgaaga
     2581 aagcacgaaa ataacctgac gtaaattgtt agatactcct caaa
//
LOCUS       DASSJH010000380         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_950400, whole genome shotgun sequence.
ACCESSION   DASSJH010000380 DASSJH010000000
VERSION     DASSJH010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950400"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VFJ67_08390"
     CDS             <1..1249
                     /locus_tag="VFJ67_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7290439.1"
                     /translation="FELTPRTYSPWIRLSFPAGLGIKVHGICRFYINTLDNGFDMYVT
                     PINIDPENPALPISHPFVYSIYLAKLQGPFGTLGLAEDTWALNEGVIDEDAFLEQAYL
                     LYEEREKMFFNTLEKTPSGLCTCVFDTTDRVQHMFFRCLDDGHPANRGKETEKYRKVI
                     EELYVRMDGLIGKALEYADNQTVLMVISDHGFTQFKRGVNLNSWLFQNGYLKLRDGRT
                     TSGDWFKDVDWEGTRAFSLGLAGIFINRKGREMRGTVEEGEGVSLLKAELVRKLTGLR
                     DEEYGAVSIREVVDTDAASTGPYKHDAPDLLIGYNAGYRSSWTCAVGRVTENVFEDNT
                     KHWSGDHCVDPKIVPGVIFSNRRIVKDNPHLNDMAPTVLKLFGVGIPNYMKGKPLLED
                     DVNAAPGAAGEPGEEERKRARAV"
     gene            1318..>2731
                     /locus_tag="VFJ67_08395"
     CDS             1318..>2731
                     /locus_tag="VFJ67_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7290440.1"
                     /translation="MKERFRTSQVNTFIFSLFITLLLASAACRESEPTSHAGTGVTDK
                     KMIIIGFDGMDPKIVKNLMAEGKLPNFRKLSEEGGFKELATSVPPQSPVAWSNFITGM
                     NPGGHGIFDFIHRDPENIFPYLSTSRTESSEKTVRIGKWVLPLSSGKVSLLRQGKAFW
                     EYLSENGINTTIFRVPANFPTVDDEAHQISGMGTPDIQGTYGTYSYYTDEDTERFGNT
                     SGGRVYPVDVFRNKVRGRIPGPINTFRKDASESRVDFTVYIDPENPVAKISVQDKEII
                     LKEGEWSDWIRINYELLPYVQKVSGIARFYLKEVRPNFKLYVTPVNIDPSKPALPISS
                     PEDYSEKIAENVGLFYTQGIPEDSKALSEQVLEDGEFLDQTNIAFGEEQDMLGYELGR
                     FRSGLLLFYIGRVDQLSHMFWRTMDPKHPAFEPGSKLKSVIENAYIEMDGVLGRVLDS
                     IDGDTTVIVLSDHGFAPFYRA"
BASE COUNT          698 a          715 c          785 g          533 t
ORIGIN      
        1 attcgagctc accccgagaa cgtattcgcc gtggataagg ctctccttcc cggctggact
       61 gggaataaag gtgcacggca tatgccgctt ttatataaac acgctggata acgggttcga
      121 tatgtacgtg actcccataa acatagaccc ggagaacccg gctctcccca tatcccatcc
      181 gttcgtatat tccatatacc tggcgaagct ccagggcccc ttcggcacgc tggggctcgc
      241 tgaagataca tgggctctca acgaaggcgt catagatgaa gacgcgttcc tcgagcaggc
      301 gtacctgctt tacgaggaga gggaaaagat gttctttaac acgctcgaga aaaccccgag
      361 tggcctctgc acatgcgttt tcgatacgac ggacagggtc cagcacatgt tcttccggtg
      421 cctcgacgac gggcaccccg cgaacagagg aaaagagacg gaaaaataca ggaaagtaat
      481 agaagagctg tatgtcagga tggacggact catcgggaaa gcgcttgaat acgcggacaa
      541 ccaaaccgtc ctgatggtga tatccgacca cgggttcacg cagttcaaac gaggcgtgaa
      601 ccttaacagc tggctctttc agaacggcta cctcaagctg agggacggac gcacgacgag
      661 cggcgactgg ttcaaagacg ttgactggga gggcacgagg gcgttctcgc tcggtctcgc
      721 gggcattttc attaaccgga aaggaagaga aatgagggga actgtggagg aaggggaagg
      781 ggtctcgcta ctcaaagccg agctcgtaag aaagctcacc gggctcaggg acgaagagta
      841 cggtgcggta tccatacgtg aggtcgtgga tacggatgcg gcttcgactg ggccttacaa
      901 gcacgacgcc cccgacctgc tcataggtta taacgccggg tacaggagct cctggacgtg
      961 cgccgtggga agggttaccg aaaacgtatt cgaagacaat accaagcact ggagcggcga
     1021 ccactgcgtc gatccgaaaa ttgttccggg cgtcatattc tcaaaccgca ggatagtgaa
     1081 agataaccca cacctgaacg acatggcccc tacggtgtta aagctgttcg gcgtcggaat
     1141 acccaactat atgaaaggga agcctctcct cgaagatgac gtaaacgccg ctccgggagc
     1201 agcaggcgag cccggagaag aagagaggaa gagagcgaga gcggtttaaa atcccgggga
     1261 cgtcatatca ttgtttgacc tgatacgagt gagataaaac ataaacggca tccacagatg
     1321 aaagaaagat tcaggactag tcaggtaaat acatttatct tctccttatt catcacgctc
     1381 ttgctcgcat ccgccgcgtg ccgggaaagc gagcccactt ctcacgccgg gacaggcgtg
     1441 acggacaaga agatgatcat cattgggttt gacggcatgg acccgaaaat tgtaaagaac
     1501 ctcatggcgg aggggaaatt acccaacttc aggaagctca gcgaggaagg aggattcaag
     1561 gagctcgcga cgagcgttcc cccccagagc cctgttgcct ggtcgaattt cattacaggc
     1621 atgaacccgg gcggccacgg catattcgac ttcatacaca gggaccccga gaacatattc
     1681 ccgtatctct cgacatccag gaccgaatcc tcggaaaaga cggtcaggat cggcaagtgg
     1741 gtgctgcctc tttcctcggg caaggtttcg ttgctgcgtc agggtaaggc gttctgggaa
     1801 tacctatccg agaacggcat caatacgact attttccgcg tgcccgctaa tttccccacg
     1861 gtggacgacg aggctcacca gatctccgga atgggcacac ccgatatcca gggcacgtac
     1921 gggacttact catactatac ggatgaagat accgagagat tcgggaacac atccggcggc
     1981 agggtatatc ctgtagacgt attcaggaac aaggtccgcg gcagaatccc ggggccgatc
     2041 aacacgttca ggaaagacgc ttccgaaagc agggtcgatt tcacggtgta catagacccg
     2101 gagaatccgg tcgcgaagat ttccgtccag gacaaggaga tcatactcaa ggaaggcgag
     2161 tggagcgact ggatcaggat aaactacgag ctgctccctt atgtccaaaa ggtgagcggc
     2221 atcgcgcgtt tttacctgaa ggaggtcagg cctaatttca aactgtacgt cacgcccgta
     2281 aatatcgacc cgtccaagcc cgcacttccg atctcgtcgc ccgaggatta ctcggagaag
     2341 atagcggaga acgtggggct cttttatacg caggggatac cggaggactc gaaggccttg
     2401 tcagagcagg tgctcgagga cggcgagttt ctcgatcaga ccaatatagc gttcggcgag
     2461 gagcaggaca tgctcggcta cgagctcggg aggttccgct cggggctcct cctcttctat
     2521 ataggcaggg tcgatcagct ctcccacatg ttctggcgca ccatggaccc gaagcacccc
     2581 gcatttgagc cggggagcaa gctgaagtcg gtgatagaga acgcttatat cgaaatggac
     2641 ggtgtgctcg ggcgcgtgct cgactctata gacggggata cgaccgtcat cgttttgtcc
     2701 gaccacggtt tcgccccgtt ttacagggct t
//
LOCUS       DASSJH010000381         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_201803, whole genome shotgun sequence.
ACCESSION   DASSJH010000381 DASSJH010000000
VERSION     DASSJH010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201803"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VFJ67_08400"
     CDS             <1..1318
                     /locus_tag="VFJ67_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7290441.1"
                     /translation="VSNTYMNDILSIAPGLGRLRSVIPMDNIEDVCRGYATGQAMENI
                     SPDSLLEILFTSGTTGDPKGVMLSHRNIMSNVDDVHRTINYGPADRAFSVLPVHHSYE
                     CTGGLISTFGSGMSVFYGRGLKPRDLLQDMKLARPTIWLSAPLILEKLYQRIHKEVTG
                     QTGIKKFLSSVAPRSFIKRKIRQSLGFDDIRLIISGGAALPGWLSSGLSEYGLDVIQG
                     YGLSEASPLITVNPPSSPRHESIGMVIPSNEVEIRDIDSEGNGEITAKGPNIMLGYYR
                     NSTATDETLTRDGWLLTGDIGYFDEDGYLYITGRKKFVIVTKGGKNVYPEEIEEKLTK
                     SPLIEEALVFSPDDAQIQALVFPQMEEAQKRIERMGEQVTPENIWLLLRHEIRNTNRE
                     LEPYKKIGHFAVRLEEFPKTTTRKIKRYLFKDLRLTQDTRIFSNDV"
     gene            1478..>2921
                     /locus_tag="VFJ67_08405"
     CDS             1478..>2921
                     /locus_tag="VFJ67_08405"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7290442.1"
                     /translation="MDTNKMTVFTRKGLVALTVTLALFGALWTGCVQPGQDQKSDNTG
                     VSSPQTPETLLSLVSGIIRQRVDRPQTPKSFVVDNTTIKFYSSLPALYRNTDYRPVWV
                     GESGPKPECRDLADAVRNSYKEGLAPEKYNLKEIDYTLARVQSASASGKLPPPELLAD
                     LDVLLSNSFLKYASDLLYGQISPAQIDLELVFGERPVDLNALLLAAVNDNKINQTLTG
                     LLPDYPVYGRLKTALAEYRGYEAEGGWKPIPGGDKLRKGARGERVTALKERLVVTGEL
                     DNSALGSNVFDAPVEQAVREYQETNGLYVDGVVGDSTLESLNVPAGERVSQIELTLER
                     WRMLPKSLGPKFVLVNIANYHLYAVKDNRDAVIMRIVVGKPKWNTPIFSEQMTHIVLN
                     PAWNIPPSIFRDDIAPLIKSDPDYMANRNIQAVGLQMEQTENADETEIASAKEEYISK
                     VLSGNYRLRQNPGPSNPLGRVKFLFPNKHSV"
BASE COUNT          789 a          774 c          807 g          551 t
ORIGIN      
        1 cgtctcaaac acgtacatga acgacatatt atcaatagct cccgggctgg gccgcctgag
       61 aagcgttata ccaatggaca atatcgaaga tgtatgccgg gggtacgcaa cggggcaggc
      121 gatggagaac atatcgcctg acagcctgct cgaaatactt ttcacatcgg ggacgaccgg
      181 agacccgaaa ggtgtcatgc tcagtcacag gaacataatg tcgaacgttg acgacgtgca
      241 caggacaata aactacggcc cggctgacag ggcgttctcc gtcctgcccg tacaccactc
      301 ctacgaatgc acaggggggc ttatatcgac attcggtagc ggcatgagcg tcttttacgg
      361 gagggggctt aagccgaggg acctgcttca ggacatgaag ctcgccaggc cgactatatg
      421 gctgagcgcg cctctgattc tcgaaaagct ctatcagagg atacacaagg aggtcacagg
      481 ccaaacgggg ataaagaagt tcctgtcgag cgtcgcgccc cggagcttta taaaaagaaa
      541 gatcaggcag agcctcggct tcgacgacat caggctcatc atttcgggag gagcggcgct
      601 tccgggctgg ctctcctctg ggctctccga atacgggctc gacgtgatac agggttacgg
      661 tctttcggag gcatccccgc ttattaccgt gaacccaccc tcgagcccca ggcacgagag
      721 tatcggaatg gtgataccta gcaacgaggt cgagatcagg gacatcgaca gcgaaggcaa
      781 cggcgagata acggcgaaag gccccaacat catgctgggg tattacagga actcgacggc
      841 tacggatgag acactcactc gggacgggtg gctgcttacg ggcgacatag gttatttcga
      901 cgaagacgga tatctctata taacgggaag gaagaagttc gtgatcgtta cgaagggtgg
      961 aaaaaacgta taccccgagg agatcgagga aaagctgacg aaatccccgc tcatagaaga
     1021 ggccctcgta ttcagcccgg acgacgcgca gatacaggcg ctcgtattcc cgcaaatgga
     1081 ggaggcccaa aagaggatcg aacgcatggg agagcaggtt acaccggaga atatatggct
     1141 cctcctcagg cacgagatca ggaatacgaa cagggagctt gagccgtaca agaagatagg
     1201 acacttcgcg gtgagactcg aagagttccc caagaccaca acccgaaaaa taaaacgcta
     1261 tctttttaaa gacttacgcc ttactcagga tacccggatt ttttcgaatg acgtatgaat
     1321 ttcacgctcg ttcgtatagc caatatatga gtttggcatt ataataagtg ggcggaatgg
     1381 ctgaaattta attaacatgc actcaaattt cagctattat tgattaaatc ggggttatgg
     1441 ataacggact tgggtacagt tcggctcata cttattaatg gatacgaata aaatgaccgt
     1501 atttacacga aagggactcg ttgcgcttac ggttacgctc gcactcttcg gcgctctctg
     1561 gaccgggtgt gtccagcccg gacaggatca gaaatctgac aatacgggtg tttcttctcc
     1621 ccagacccct gaaacgctcc tcagcctcgt aagcggaatc atcaggcaaa gggtggacag
     1681 accgcagact ccaaaatcat tcgtcgtcga taacacaacc attaaattct acagctcgct
     1741 gcccgcttta taccggaata ctgactacag gcccgtatgg gtcggtgaga gcggcccgaa
     1801 gcccgaatgc cgggacctgg ccgatgccgt ccgaaacagc tacaaggaag gactcgcccc
     1861 ggagaaatac aacctgaaag agatcgacta tacgcttgcg agagtacagt cggcgagcgc
     1921 ctcgggcaaa ttaccaccgc ccgaattact tgccgacctg gacgtactat tatcgaactc
     1981 atttcttaaa tacgcgagcg acctgcttta cggccagata agcccggcac agatagacct
     2041 cgagctcgtt ttcggggaaa ggcccgtcga cctgaacgcg cttctcctag cggcagtaaa
     2101 cgataacaag ataaaccaga cgctaacagg gttgctgccg gattatccgg tgtacggaag
     2161 gttgaaaacc gctctcgccg aatacagggg atacgaggcc gaggggggat ggaaaccaat
     2221 acccgggggc gacaaactga ggaaaggcgc gaggggagaa cgggtcacgg cgctcaagga
     2281 aaggctggtc gtgacaggag agctcgacaa ctcggcactc ggaagcaacg ttttcgacgc
     2341 cccggtcgaa caggccgtca gggagtatca ggagacgaac ggactctacg ttgacggcgt
     2401 cgtgggcgat tccactctcg aatccctgaa cgtgcccgcc ggggaacgcg taagccagat
     2461 cgagctcacg ctcgagcgct ggcggatgct gcccaagagc ctcggtccga aattcgtgct
     2521 cgtaaacatc gccaactacc acctgtatgc ggtcaaggat aacagggacg ccgtcattat
     2581 gagaatcgtc gtcggcaagc ccaaatggaa cacgcccata ttcagcgagc agatgaccca
     2641 catcgtgctc aaccctgctt ggaacatacc gcccagcatt ttcagggacg atatagcccc
     2701 cctgatcaaa tctgacccgg attacatggc caacagaaac atacaggcgg tcggactgca
     2761 gatggagcag acggaaaacg cggacgagac ggagatcgct tcggccaagg aggaatatat
     2821 atcaaaagtg ctctccggca attacaggct ccgccagaac ccgggcccgt caaacccgct
     2881 cggcagggtg aagttccttt tcccgaacaa gcactccgtc t
//
LOCUS       DASSJH010000382         9573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1060822, whole genome shotgun sequence.
ACCESSION   DASSJH010000382 DASSJH010000000
VERSION     DASSJH010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060822"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFJ67_08410"
     CDS             complement(<1..389)
                     /locus_tag="VFJ67_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290443.1"
                     /translation="MRNGRLFALALLLALAFGIAGGCNNGGGGGIDEEPPPQKPPFDL
                     ETAIDLGELSLVAYNQLQECIDSGKDAITVPSPWTLEEVIYEGVDSDLNDTCLDDKGV
                     VPIAFIATNGSGIYLAFRGTSNVSDALS"
     gene            556..1209
                     /locus_tag="VFJ67_08415"
     CDS             556..1209
                     /locus_tag="VFJ67_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290444.1"
                     /translation="MKTLRFGLIAGIIWTLVSLMLIAGSVIIGYRFAWVELDYRILSG
                     AVLLAAKALGMGDAVSGLAFYAETGLPVTPVHVVSCAAFSFADGFTSGLVIALVYNAL
                     DGGKGGSEIRKAVCFGIAAGAALGIASGLLALTCRAYGVGITSFDFTVRPVWLMFHTW
                     SKLGIPDFLLPLWNSYMYFPNNLAGALAWAAWGFIDGLVEGSALAYVFLKLREVYKT"
     gene            complement(1784..2170)
                     /locus_tag="VFJ67_08420"
     CDS             complement(1784..2170)
                     /locus_tag="VFJ67_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HET7290445.1"
                     /translation="MSDITNREDIELLVRKFYGRMMDDPIIGFFFTYYAKIDLESHLP
                     VISDFWETVLFQKPVYEGGAQAMNVHLDLNKKVKFRNQHFTRWLYLFHRSVDELFEGP
                     NAVKAKERSASIAELMKKRMGVLPDC"
     gene            2509..4488
                     /locus_tag="VFJ67_08425"
     CDS             2509..4488
                     /locus_tag="VFJ67_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7290446.1"
                     /translation="MRPEEPPIDNISEPCQVRMREIDEESVLRNLMELTASATGERFF
                     YSIVEGLSKALGTEGAWCTVLNKEKNTLRALAFLMQGVWYDDYEYDFTGSVCEEVILA
                     DKLVHHPDNIMDIYSNSETLRSFKAVSFMGAPLKDKDGVTLGYLSVLDTRPIPVDNHL
                     IGIFNIFADRAAAELSRIRAEATIREREGKLSSLFESAMDSILELDSGLRINLVNSAA
                     EKLFGFGAGSLTGNDFTRFLTKESREKLLRIIKELHMQPDDKKYIWIPGGLEAVTSSG
                     GRFSAEATISTYEAGRKIFYTLILRNVNERIEATKLIRSLMDEAKYLEEEIKDLGNFD
                     EIIGRSAPMKEVMQDVKQVAGTGSTVLILGETGTGKEVIARTIHKLSGRSERPLIRVN
                     CAAIPAHLIESEFFGHEAGAFTGATRKRDGRFTLADGGTIFLDEVAELSLDLQAKLLR
                     VLQEGEFEPVGSSSTKKVDVRVLAATNRDLKKSVGKGEFREDLYYRLNVFPINLPPLR
                     ERGEDIEIFARSFAEKFARRMGREFEPLHDDCIRRLTSYSWPGNVRELQNVIERAVIT
                     SRGSRLDLGRAFPESSDPAAPDGCGETQTTRAGSILTLEEMKGFERENILRALVSTHG
                     RIYGEGGAAELLGMNPSTLRSRMKALGINKYENGV"
     gene            5053..5460
                     /locus_tag="VFJ67_08430"
     CDS             5053..5460
                     /locus_tag="VFJ67_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290447.1"
                     /translation="MKKVYIIGAFYVLLVFALSVLSISFLAAAQEGALDGKTFSGEMG
                     ETGKKGDKEELVFKDGKFTSVACEQYGFGDAPYTTTVNGDTTTFEADTVSAKEGKMHW
                     SGTMKGSELTGTAVWTKEGQAPVEYWYKTTLEK"
     gene            complement(5621..5887)
                     /locus_tag="VFJ67_08435"
     CDS             complement(5621..5887)
                     /locus_tag="VFJ67_08435"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HET7290448.1"
                     /translation="MQFMITQLHDPQDCPKDIGGFRKVLINEKVSGLTLKNVYVEHGS
                     HTIIYIVETDDYNNIVKFLEPGMMTCTSSITPVSSMAMAEIMKK"
     gene            6208..7266
                     /locus_tag="VFJ67_08440"
     CDS             6208..7266
                     /locus_tag="VFJ67_08440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7290449.1"
                     /translation="MSIDNDKLYELLGKAVVDFGATFHAALVVIGDRLGLYKALAEAE
                     SLTPAELAEKTGTSERYVREWLSCQAAGGYVSYRVETKSFYLTEEQAFVLADETSPAF
                     VPGAFQVAVSSVKSQSEIEKRFRNGNGFGWHEHDHGLFEGTERFFRPNYSANLVSSWI
                     PALNGVEDKLREGGLVADVGCGHGASTILMAKTYPKSTFIGYDYHKPSIEWARKAAAD
                     AGVSGNTSFEVAASKNYPGTDFDLVTFFDCLHDMGDPAGASAHVLKSLSKDGSWMIVE
                     PFANDNLEDNLNPVGRAYFGASTLICTPASLAQEVGAALGAQAGESRLREVVTKGGFS
                     RFRRAAETPFNLIFEARP"
     gene            complement(7283..7450)
                     /locus_tag="VFJ67_08445"
     CDS             complement(7283..7450)
                     /locus_tag="VFJ67_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290450.1"
                     /translation="MEYDKALRKDEILKRACTELDSGFRMTDKRKRIVPLTLSLSHAG
                     EREFICKGEGD"
     gene            7536..8234
                     /locus_tag="VFJ67_08450"
     CDS             7536..8234
                     /locus_tag="VFJ67_08450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7290451.1"
                     /translation="MQEKFDPVKRFNQRASVYDEDINKIIPGYDALHSNALHLLETSL
                     GDDAFLLVAGVGTGNETVAAALRNPHWRVTGFDVAENMIKTATAKIEKHGLGSRVELV
                     HGVLDDVLQESFDAATALLVMHFIPFSDKHAFLKGINLRLEPGGLLVTADFTCDRESY
                     EYETFERAWEEYMLATTEKKEVDERLWHTRHDLDILSHQETLEHLRRAGFTNTRLFWK
                     SLIFSAYISEKEDL"
     gene            complement(8341..9039)
                     /locus_tag="VFJ67_08455"
     CDS             complement(8341..9039)
                     /locus_tag="VFJ67_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HET7290452.1"
                     /translation="MILSHKYKFIFIKTRKTAGSSIQIYLSHLCGEKDVLSPIDRPEQ
                     PYTPRNFRGLFNPLPELAERQSAGQVLKTLGRFFTLKKFQSHIKAREVRDRIPREIWD
                     SYFKFTVERNPWDKTLSHYHFVRQRYKKCDKNISFEKYLDVADLPYNYKKYTDPDGRI
                     IVDRVLRYENLNGELADVFGMLGVPFEGTLGATEKSHYRQDRRPYREVYTPAWKDAVE
                     RLFSREIELLGYEF"
     gene            complement(9148..9525)
                     /locus_tag="VFJ67_08460"
     CDS             complement(9148..9525)
                     /locus_tag="VFJ67_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290453.1"
                     /translation="MIEIKKYIFSAGDPARKPRKGRGTVTFYCVMHIDRYSNRILSAV
                     FSDRKKAEAYRNMSPMKLHIEPEEVGGYTGSAALWISNEPGPGGVSSFVKLHSSYKEA
                     LASSRRGRPRAVLIGDGGRVYSS"
BASE COUNT         2206 a         2615 c         2600 g         2152 t
ORIGIN      
        1 gaaagagcgt cagacacatt ggatgtcccc ctgaaggcga ggtatatgcc gctcccattc
       61 gtcgctatga acgcgatggg cacgactccc ttgtcgtcga ggcatgtatc gttgaggtcg
      121 ctgtcgacgc cctcgtaaat gacctcctca agggtccagg gggaagggac cgtgatcgcg
      181 tcctttccgg aatcgatgca ttcttgaagc tggttgtaag cgaccaagct cagctcgcct
      241 aggtcgatcg ctgtttcgag atcgaacggc ggtttctggg gcggcggctc ctcatcgatg
      301 ccccctcccc cgccgttatt gcatccgccc gcgatgccga acgcgagcgc cagcaaaagc
      361 gcgagcgcaa ataatcttcc gttcctcatg ctatcctcct tcttttgtct cttgataaat
      421 ataataaccg aatcgcgggg gacattcgca gcgtcgtgaa attatcgcaa aattctgcgc
      481 ttaacggtgg ggcgtattcg cggcaggcgc tcacggctta cgccccccgc gcccgtcgtg
      541 taagatgttc ttcggatgaa gacgctcagg ttcggattga tcgcgggaat aatctggacg
      601 ctcgtatcgc tcatgcttat agccgggtcc gtgataatag gctacaggtt cgcctgggtg
      661 gagctggatt accggattct ctctggtgcg gtgcttctcg ccgcgaaagc cctcggcatg
      721 ggcgacgccg tgagcggtct cgcgttctac gccgagaccg gacttcccgt gacccccgtc
      781 cacgtcgtat cctgcgcggc tttctcgttc gctgacgggt tcacgagcgg cctcgtgatc
      841 gcgctcgttt ataacgctct cgacggaggc aaagggggta gcgaaataag gaaggccgtc
      901 tgtttcggca tagcggcggg cgcagctctc gggattgcct cggggctcct cgcgctcacg
      961 tgccgtgcgt acggcgtagg cataacatcg ttcgacttca ccgtgaggcc cgtgtggctg
     1021 atgtttcaca cgtggtcaaa gctggggata cccgacttcc ttcttccgct atggaattcg
     1081 tacatgtatt ttccaaacaa cctcgcgggc gcgctcgcct gggccgcgtg gggcttcatt
     1141 gacggcctcg tcgaaggatc ggcgctcgct tacgtgtttt taaaattgcg cgaggtttat
     1201 aaaacctgag cgtccactac ggacgcacaa acagcggggc ttcgagtggg gttgtttcct
     1261 ttcccgacat tacagaattt acgttaggaa aatcgattgt ttacgcgtca aaaacatttt
     1321 tgggtacgaa tgcaagctag cgcgcattcg gacgccttac cttatccaaa cggcgcattt
     1381 tacatcgcac atcattagac tacatcgccc tatcgggtgg aataaaaatg ttttagcgta
     1441 aacattagat ttttagtaaa ttctgttctg tcttttcata aaacacacta cgtgtgtgat
     1501 ttttgcttct ttccgggcta cggcaggctc cgccctgacg agtcatcaag gaaaagaaga
     1561 acacacacat tgtgtgattt acaattcttc cgaatctcct caggtcaatc cagtaaccgt
     1621 ggaagtttcg tcctttgtat tttatcccct ccttagaaaa aggagggtta gggaggattt
     1681 agtctttacc cgttcaatcc ttcgacaggc tcaggacgaa caggtcgttc ttccaatttc
     1741 acgagaaatc actccaaact cgtatgtccg cggcggctcg ccgtcagcag tcgggcagga
     1801 cgcccattct ttttttcatc agctccgcga tcgaagcgga gcgctccttg gccttcacgg
     1861 cgttcggtcc ctcgaacagc tcgtcgacgg atctgtgaaa gaggtagagc catctggtga
     1921 agtgctggtt tcggaacttg accttcttgt tgaggtcgag gtgcacgttc atcgcctgcg
     1981 cgcccccctc gtaaacaggc ttctggaaaa gcacggtctc ccagaaatcg cttatcaccg
     2041 gcaggtggct ttcgaggtct atcttggcgt aatacgtgaa gaaaaacccg atgatcgggt
     2101 catccatcat gcgcccgtag aatttcctca cgagcagctc gatgtcttct ctgtttgtga
     2161 tatcgctcat agcggcggaa aattccttcc agagggattc taccagattt attcccgccc
     2221 cgcaggtgag aaatctatct ttagtctttt ccttttaatt ccctccttgg aaaaaggagg
     2281 gttagggagg atttatcttt ttgccggatt ttccattctc cgggaaaaac ataaaaagac
     2341 gagattctga aacaagttca gaatgacaga taaaaaaatc caaatacccc caaggccgaa
     2401 ggaaggaccc cctcccccct ttatttccgg taaactcttg tgttagactt ttatcgttga
     2461 ccggcacgta ttgcatacag cctgccggtc ctcaaggggc aacacggcat gaggccggag
     2521 gaaccgccta tagacaatat aagcgaacct tgccaagtca gaatgaggga aatagacgaa
     2581 gagtccgtgc tccggaacct gatggagcta acggcctccg ctacgggcga aaggttcttc
     2641 tactcgatcg tcgaaggtct ctccaaggcc ctcgggaccg aaggcgcatg gtgcaccgtg
     2701 ctcaacaagg agaagaacac cctccgggcc ctcgccttcc tcatgcaggg cgtgtggtac
     2761 gacgactacg aatatgactt cacgggcagc gtgtgcgagg aagtaatcct cgccgacaag
     2821 ctcgtccacc atccggacaa cataatggac atctactcca acagcgaaac tctcaggtcc
     2881 ttcaaggctg tgagcttcat gggcgctcct ctcaaggaca aggacggcgt cactctcgga
     2941 tatctgtcgg tgctcgacac gcggccgata ccagtcgaca accatctcat aggaatattc
     3001 aacatattcg cggacagggc cgccgccgag ctatcgagga tacgcgccga ggcgaccata
     3061 agggagcgcg agggcaagct ctcgagcctg ttcgaatccg cgatggactc gatactggag
     3121 ctcgacagcg ggctcaggat caacctcgtc aactcggccg ccgaaaagct gttcggcttc
     3181 ggagcgggaa gcctcaccgg gaacgacttc acccgcttcc taacgaaaga gagcagggaa
     3241 aagctcctta gaataataaa agagctgcac atgcagccgg acgataagaa atatatatgg
     3301 ataccgggcg gcctcgaagc agtgacctca tcgggagggc gtttctccgc ggaggcgacc
     3361 atatccacgt acgaggccgg caggaaaatc ttctacacgc tcatactcag gaacgtaaac
     3421 gagcgcatag aggcgacgaa gctgatacgc tcgctcatgg acgaagcgaa atatctggag
     3481 gaggagataa aagacctcgg aaatttcgac gagataattg gcaggagcgc cccgatgaag
     3541 gaggtcatgc aggacgtgaa acaggtcgcc gggaccggga gcaccgtgct catactgggc
     3601 gagaccggca cgggaaagga agtgatagcg aggacgatac acaaactgag cgggaggagc
     3661 gagaggccgc tcataagggt caactgcgcc gctatccccg cgcatctcat tgagagcgaa
     3721 ttcttcgggc acgaagcggg cgcgttcacg ggggcgaccc ggaagcggga cggaaggttc
     3781 accctcgccg acggcgggac catattcctc gacgaggtcg ccgaattgtc cctcgacctc
     3841 caggcgaagc tcctgcgcgt gctgcaggaa ggcgaattcg agccggtcgg gagctcgagc
     3901 acgaagaagg tggacgtgcg cgtgcttgcg gcaacgaacc gcgacttgaa aaagagcgtc
     3961 gggaaaggcg agttccgcga agacctctac tacaggctga acgtattccc gataaacctt
     4021 cccccgctcc gcgagagggg cgaagacata gagatcttcg cgaggtcgtt cgccgaaaaa
     4081 ttcgcgagga ggatgggacg ggaattcgag cccctgcatg acgactgcat caggaggcta
     4141 acgtcctact catggcccgg aaacgtcagg gagcttcaaa acgtgataga gagagcggtc
     4201 atcacgtccc gggggagccg cctcgacctc ggccgcgcgt tcccggaatc gtccgatccc
     4261 gcagcgcccg acggatgcgg tgagacgcaa acgacgcgtg cggggtctat actcactctc
     4321 gaagagatga aaggcttcga gagggaaaac atattgaggg ctctcgtcag cacgcacggc
     4381 aggatatacg gagaaggcgg cgctgcggag cttctcggca tgaacccctc gacgcttcgc
     4441 tcgcgcatga aagccctcgg cataaataag tacgagaacg gcgtgtaagc acacatagtg
     4501 tgttttatga tccttcctta cctctgatac tcaagccagt ggtaaagcca aagccgttca
     4561 tacttcgaca agctcagcac gaacggtttt ttttaatgat ttgtcattgt gatgtgcaga
     4621 cgcggcaagc tcgtctttgt ctttccccga catcacagaa ttggcgtaaa gaaaatcagc
     4681 tgttaacgcg ttaagaaatc tttcaggggt gtgggttgaa ttgaaagatt ttagcgttaa
     4741 ctagggattt ttagccaatt ctgtcctgtc ggttgatttt tgcttctttt catcaaggaa
     4801 aagaaggata aaaacaatag cctttcactt ttctttatct ttttataatt cattataatg
     4861 gttaatcaag gtttggagcg tgtaatttca cgctccccga ctgtgaaatc tcgcgcggcg
     4921 cttaaaatca cgccccgaat tcggccagcc ggtcgacaca atctcctgaa agcacacacg
     4981 aatttttccc tagtcttcat ggcacattaa atgcatttac atgttacata tcaaaacagg
     5041 aggtgttaag aaatgaagaa agtttatata ataggtgcct tttatgtgct tttagttttt
     5101 gccctctccg tcttatcgat ttctttcctc gcggcagccc aggaaggcgc gctcgacggc
     5161 aagacttttt cgggcgagat gggagagacg gggaagaaag gcgataagga agagctcgtg
     5221 ttcaaggacg ggaaattcac ctcggtcgcc tgcgagcagt acggattcgg agacgctccc
     5281 tatacgacga cagtgaacgg ggacacgacc acgttcgagg ccgatacagt gagcgcgaaa
     5341 gaagggaaga tgcactggtc gggcacgatg aagggcagcg agctcaccgg cacggcagtg
     5401 tggacaaagg aaggccaggc gcctgtcgag tactggtaca agaccacgct cgagaaatag
     5461 cgcgagcccg cgtcaatgat cagcctctac cataacgaat tcacctcacc ttataaattc
     5521 cctctcccca gcgtgggaga gggatagggt gagggggaac ctctctatta ctatcattgt
     5581 ttctcaacaa cgcatctcaa caacgcgcct tacttcccaa tcacttcttc ataatctcgg
     5641 ccatggccat cgatgaaacg ggcgtgatgg acgacgtgca agtcatcatc cccggctcga
     5701 ggaacttgac gatgttgtta taatcgtcgg tctcgacgat atagatgatc gtatgagagc
     5761 cgtgctcgac gtacacgttt ttcaaggtca gcccggacac cttctcgttt atcagaacct
     5821 tcctgaatcc gcctatgtcc ttcggacagt cctgcgggtc gtgaagctgc gtaatcatga
     5881 attgcatttt taacctccct gtgcggatcg tttatcgtac attttagagt gcataatcga
     5941 ttccatcaac cggacctccg caagccgtat gaataattgg ttttcctgat ctcatctatt
     6001 accggtccgc ctttattgcc aaaccgttat atttcacgta aaaagatcgc taaatctcac
     6061 gatccgctcg atctcgtggt gtgtcatata gcacgataat atgcctaact tgctgttcta
     6121 acgacatatt attccatcga ataatacctg gcacagtcca tgcacctaat agattcaaaa
     6181 tctatttcca atgaggaggt aagtaaaatg tctatagata atgataagct gtacgagctt
     6241 ttagggaaag cggtagtgga tttcggggct acgttccatg cggccctcgt ggtaataggg
     6301 gacaggctcg ggctttacaa ggctcttgcc gaggccgagt ccctcacccc cgccgagctc
     6361 gctgagaaga ccggcacctc cgagaggtac gtgagggaat ggctctcatg ccaggcggct
     6421 ggcggctacg tctcttaccg cgtggaaacg aagtcgttct acctcacgga ggagcaggcg
     6481 ttcgtgctgg cagacgagac aagccccgcg ttcgtcccgg gcgcgtttca ggtggctgta
     6541 tcttcggtca aatcccagtc ggagatcgag aagcgtttcc gcaacgggaa cggtttcgga
     6601 tggcacgagc acgaccacgg gctattcgaa ggaacggagc gcttcttcag gcccaactat
     6661 tcggccaatc tcgtgagctc gtggataccg gcgttaaacg gcgtcgaaga caagctcagg
     6721 gagggagggc tcgtggctga cgtagggtgc gggcacggcg catcgaccat actcatggcg
     6781 aagacctatc ctaaatccac gtttatcgga tacgactacc acaagccctc gatcgagtgg
     6841 gcgaggaagg cggctgcgga cgcgggcgtg tccgggaata cgtccttcga ggtagcggcc
     6901 tcgaaaaatt atccgggcac ggacttcgac ctcgtcacct tcttcgactg cctccacgat
     6961 atgggcgacc cggccggcgc ttcggcgcac gtgcttaagt cgctctctaa agacgggtcg
     7021 tggatgatag tcgagccgtt cgcgaacgat aacctcgagg acaatctgaa ccccgtcggc
     7081 agggcttatt tcggcgcgtc cactctcata tgcacgccgg cgtcactcgc gcaggaagta
     7141 ggcgcggctc tgggcgctca ggcgggcgag tcgaggctca gggaagtcgt gaccaagggc
     7201 ggattctcac gcttcagaag ggcggccgag acccctttca acctcatatt cgaagcgcgt
     7261 ccgtgaccgt cggatacaac aatcaatccc cttcaccctt acaaataaat tccctctccc
     7321 ccgcgtggga gagggacagg gtgaggggga caattctttt tcttttatcc gtcatcctga
     7381 accctgaatc aagttcagtg caggctcgtt tcaggatctc gtcttttctc aaggctttat
     7441 catattccat agtttctaac aagaattcta agctaatttt actctcctcc ctccccctct
     7501 tcccctcccc atattctcca ttacactatt accctatgca ggaaaagttc gatcccgtaa
     7561 agaggttcaa ccagagggcg tccgtttacg acgaggatat caataagatc atccccggat
     7621 acgacgcgct ccactcaaac gcgctccacc tgctcgaaac ctcgctaggt gacgacgcgt
     7681 tcctgctcgt ggcgggcgtg ggcacgggga acgagaccgt cgccgctgct ctccgaaatc
     7741 cgcattggag agtgaccgga ttcgacgtcg ccgagaacat gataaagacc gcaaccgcca
     7801 agatcgaaaa gcacgggctc gggtcgaggg tggagcttgt tcacggcgtc ctcgacgacg
     7861 tgctccagga atcgttcgac gcggcgacag cgctgctcgt aatgcacttc ataccgttct
     7921 cggacaaaca cgctttcctc aagggcatca atctcaggct cgagcccgga ggattgctcg
     7981 tcacggcgga cttcacatgc gacagggaat cgtatgaata cgagacgttc gaacgcgcgt
     8041 gggaggagta catgctcgcg actacggaga agaaagaggt ggacgagagg ctatggcaca
     8101 cacgccacga cctcgacata ctgtcccatc aggagactct cgagcatttg aggagagcgg
     8161 gtttcacgaa cacgcgccta ttctggaagt ccctcatctt cagcgcctac atttcggaaa
     8221 aagaggattt gtgaaacggg tgggcgtgag cacttacagg caaacgatct ctaacatttc
     8281 cttatattgt cattcccgaa ggctttaatc gggaatgaca aatcacaaat attcccaaca
     8341 ctaaaactca taccccagaa gctctatctc gcggctgaag agcctctcta ccgcatcctt
     8401 ccaggcgggg gtataaacct ccctgtacgg gcgcctgtcc tgcctgtaat gggacttctc
     8461 ggtagccccg agcgtgccct cgaacggcac tcccagcatg ccgaacacgt ccgcgagctc
     8521 cccgttcagg ttctcgtacc tgagcacgcg gtcgacgatg atcctgccgt cggggtcagt
     8581 gtacttcttg tagttgtagg gaaggtcggc gacgtcgagg tacttctcga aagaaatgtt
     8641 cttgtcgcac ttcttgtaac gctgcctcac gaaatgatag tgagataacg ttttgtccca
     8701 cgggttcctc tcgaccgtga acttgaagta gctgtcccat atctcgcgcg gaatcctgtc
     8761 cctcacctcg cgcgccttta tgtggctctg gaatttcttg agcgtgaaga accgcccgag
     8821 ggtcttcagc acctgccccg cgctctgcct ttcggcgagc tccggcaggg ggttgaagag
     8881 gcccctgaag ttccggggcg tgtacggctg ctccggcctg tcgatggggg agaggacgtc
     8941 cttttcgcca cagagatgag agaggtatat ctgaatgctc gagccagcag tcttgcgcgt
     9001 ctttatgaat atgaatttgt acttgtggga gagtatcatg ttccgcagcc ggacccgctt
     9061 cgggcctaca cggccctatc cggacataga aattctacca cgattcggaa taggggcaaa
     9121 taaatcttac cggccggacg tcggagccta actgctgtaa acccttcccc cgtccccaat
     9181 caggaccgcc ctgggcctcc ccctccggga agacgcgagg gcttctttgt acgaggagtg
     9241 gagcttcacg aacgatgaaa cgccgcccgg gccgggctcg ttcgagatcc agagcgccgc
     9301 gctcccggta tatcccccca cttcctcggg ctctatgtgg agcttcatcg gagacatgtt
     9361 cctgtaggcc tcggcctttt tcctgtccga aaaaacggcc gagagaatcc tgttcgagta
     9421 cctgtcgatg tgcatgacgc agtagaacgt cacggtgccg cgccccttac gtggcttcct
     9481 cgcgggatcg cccgcgctaa aaatgtattt ttttatctct atcatgaggt agaggatagc
     9541 cgaggacggt taaggcggta tgaaggcggg gtt
//
LOCUS       DASSJH010000383         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1061111, whole genome shotgun sequence.
ACCESSION   DASSJH010000383 DASSJH010000000
VERSION     DASSJH010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061111"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFJ67_08465"
     CDS             complement(<1..355)
                     /locus_tag="VFJ67_08465"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7290454.1"
                     /translation="MRKLIAPSILSADFTRLGEEVRALREAGADWIHVDVMDGHFVPN
                     ISIGLPIVEALRKADTPPMDIHLQINNPRDFAEKFIDAGGEAVRGITVQYETCPLLHS
                     IINAIKSRGVKAGVAI"
     gene            589..1749
                     /gene="metK"
                     /locus_tag="VFJ67_08470"
     CDS             589..1749
                     /gene="metK"
                     /locus_tag="VFJ67_08470"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7290455.1"
                     /translation="MPLRNFIFTSESVTEGHPDKMADQISDAVLDAMIGGDPHSRVAC
                     ETLLTTGLVVVAGEITANTIVDLSNIVRKTITEIGYTDSAMGFDANTCGLIIAVDKQS
                     PDIAMGVDAGAEKEQGAGDQGLMFGYATDETPELMPSPIIFAHKLAKRLTDTRKDGTL
                     PFLRPDGKTQVTVRYENNVPVGIDAIVVSSQHSADVTNKTIHEGILEEVIRHCINTDM
                     ISDRTKIYINPTGRFVTGGPMGDSGLTGRKIIIDTYGGWARHGGGAFSGKDPSKVDRS
                     AAYMARYVAKNVVASGLAKECEVQLAYAIGVAEPVSVMVETFGTSVVPEEKISNAIRE
                     VFDLCPSGIISTLDLLRPIYRDTASYGHFGRFGTGFTWEKTDMVNDLLKAVS"
     gene            1796..3052
                     /gene="ahcY"
                     /locus_tag="VFJ67_08475"
     CDS             1796..3052
                     /gene="ahcY"
                     /locus_tag="VFJ67_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7290456.1"
                     /translation="MKFDVKDLKLAKQGKLRVEWAAEEMPVLELISERFKKEKPLKGV
                     TIAACLHVTTETANLALTLKEGGANVVLCASNPLSTQDDAAAYLVKDHGISVFAIKGE
                     NTKTYYSHINSTLDLSPNITMDDGADLVSILHKSRTEKLKGVIGGTEETTTGVIRLRS
                     MAENGVLKYPIIAVNDANTKHFFDNRYGTGQSTIDGIVRATNRLISGTVFVVCGYGWC
                     GKGLAMRARGMGANVVVTEIDELAALEALMDGFRVMPIAEAAKIGDFFCTVTGNISVI
                     RKEHFRTMKDGAIVCNSGHFNVELDLTGLAQISSKKRMIREYVEEFTLKNRKRINVLG
                     EGRLINLAAAEGHPSSVMDMSFANQALCAEYVVKNAKKLEKRVYDVPAQVDQSVARTK
                     LKSRGIKIDKLTPEQRKYLSSWEMGT"
     gene            3072..3959
                     /gene="xerD"
                     /locus_tag="VFJ67_08480"
     CDS             3072..3959
                     /gene="xerD"
                     /locus_tag="VFJ67_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET7290457.1"
                     /translation="MDVLLDSFLSYLAVEKGLSENTLESYGRDLRKFIRSMEEAGVRS
                     AGEINYSHILDYLSLSKEKGFNATSIVRSIVSIRQFFKYLLMEKIVDADPASGIGTPK
                     MRKGLPVVITLDEVEALISAPDESTPEGLRDAAMLEALYATGIRVSELVGLKLNDVNF
                     ELGFVVVYGKGSKERIVPMGDKARQKLLEYLGTSRPAMLKGREAKALFVTRLGRGMTR
                     QGFWKIIKHYSLKAGIARKISPHTLRHSFATHLLERGADLRTIQIMLGHSDISTTQIY
                     THVESERLKVIHKKYHPRS"
BASE COUNT         1029 a         1080 c         1164 g          759 t
ORIGIN      
        1 ttatcgccac cccggccttc actcccctcg acttgatagc gttgataatg ctgtggagaa
       61 gcgggcacgt ctcgtactgg accgtgatgc cgcggacggc ttctcctccg gcgtctatga
      121 acttctcggc gaaatcccgg gggttattta tctggaggtg gatgtccatg ggaggcgtat
      181 cagctttccg gagcgcctct acgatgggca gccctatgct gatgttgggg acgaagtgcc
      241 cgtccataac gtccacgtgt atccagtcgg ccccggcctc cctgagcgca cggacctcct
      301 ccccgagcct cgtgaaatca gctgataata tggagggcgc gattaatttc ctcattttat
      361 tcatatcctt acgcctattt gcttcccgtg cggacgattc aggttctaaa tccagggtac
      421 ggattatacc atgcctcgtc gcgtgtgaga aaaaagtttc atatgaccat ttttcccccc
      481 ggtaacacgc acggcgggcg gcccggccgg tttcttgaca gaccccttaa aactatttac
      541 catatccgct cttattgaac aatttccgct cataacaagg atatttgcat gccgctaagg
      601 aattttatct ttacctcgga atccgtaacc gagggacacc cggacaagat ggccgaccag
      661 atatcggacg cagtgctcga cgccatgata ggaggcgacc ctcacagcag ggtagcctgc
      721 gagactctcc ttaccacggg gctcgtagtc gtggcgggcg agataacggc caacaccatc
      781 gtcgatctct cgaacatagt aaggaaaacg atcacggaga taggctacac ggacagcgcg
      841 atgggctttg acgcgaacac gtgcgggctg ataatcgccg tcgacaagca gtcgcccgac
      901 atagccatgg gagtcgacgc cggagccgag aaggagcagg gagccgggga ccagggcctc
      961 atgttcggtt acgctacgga cgaaacgccc gagctcatgc cgtcgcccat tatattcgca
     1021 cacaagctcg caaagagact cacggacacg aggaaagacg ggacgctgcc gttcctgaga
     1081 ccggacggga agacgcaggt gacggtcagg tacgagaaca acgttccggt cggtatcgac
     1141 gccatagtgg tttcgtccca gcattcggcg gacgttacga acaagaccat acacgaaggc
     1201 atattagagg aagttatcag gcactgcata aacacggata tgatttccga cagaacgaag
     1261 atatacatca atcccacggg gaggttcgtt acgggcggcc ccatggggga ctcgggcctc
     1321 accgggagga agatcatcat agacacctac ggaggctggg cgaggcacgg cgggggagcc
     1381 ttttcgggga aggacccgtc caaggtggac aggagcgccg cttacatggc gcgttacgtg
     1441 gccaagaacg tggtcgcttc agggctggcg aaggagtgcg aggtacagct cgcctacgct
     1501 ataggagtgg ccgagcccgt atccgtaatg gtcgagacat tcggcacgtc ggtcgttccc
     1561 gaagaaaaga tttcgaacgc cataagggag gtgttcgacc tctgtccttc ggggatcata
     1621 agcacactcg atctcctgag acccatatat cgtgacactg cgtcttacgg acatttcggc
     1681 agattcggca cggggttcac gtgggaaaag accgacatgg taaacgacct gcttaaagcg
     1741 gtatcctgac agtccccagg ggacacacac tcaatttatt tcaacggagg caataatgaa
     1801 attcgacgta aaagacctta aactggccaa acaggggaaa ctcagggtcg aatgggccgc
     1861 tgaggaaatg cccgtgctcg aattgataag cgagaggttc aagaaggaga agccgcttaa
     1921 gggcgttaca atagccgcct gccttcacgt gacgacagag acggccaacc tcgctttaac
     1981 cctcaaggaa gggggcgcga acgtcgtgct ctgcgcctct aaccccctca gcacgcagga
     2041 cgacgcggcc gcctacctcg taaaagacca cggaatatcc gtcttcgcga taaagggcga
     2101 aaacacgaaa acgtattaca gccacataaa cagcacgctc gacctctccc ccaatatcac
     2161 gatggacgac ggggccgacc tcgtctccat actccacaaa tcgaggaccg agaagctgaa
     2221 gggcgtcatt ggcggaacgg aggagactac cacgggggta ataaggctcc gcagcatggc
     2281 cgagaacggg gtgcttaaat atccgataat cgccgtgaac gacgccaaca cgaaacactt
     2341 cttcgacaac cgctacggca cggggcagag caccatagac ggcatagtaa gggcgacgaa
     2401 caggctcata tcgggcacgg tgttcgtcgt gtgcggctac gggtggtgcg ggaaaggtct
     2461 tgccatgagg gcccgcggaa tgggagcgaa cgtcgtagtg acggagatcg acgagcttgc
     2521 cgctctcgaa gccttaatgg acgggttcag ggtcatgccg atcgcggaag cggcgaagat
     2581 aggcgacttc ttctgcaccg tcacggggaa cataagcgtc ataaggaagg agcatttcag
     2641 gacgatgaag gacggcgcga tagtctgcaa ctccgggcac ttcaacgtgg agctcgacct
     2701 gaccgggctc gcccagatat cgtccaagaa acgcatgatt agggaatacg tcgaggaatt
     2761 cacgctcaag aacaggaagc gcataaacgt cctcggcgag gggaggctca tcaacctcgc
     2821 ggcagccgaa gggcacccgt cgagcgtgat ggacatgagc ttcgccaatc aggccctttg
     2881 cgccgaatac gtcgtgaaaa acgcgaagaa gctcgaaaag agggtttacg acgtacccgc
     2941 tcaggtggac cagtcggtcg cccgtacgaa gctgaaatcg aggggcatca agatagacaa
     3001 gctcactccc gagcagagaa aataccttag ctcgtgggaa atgggcacct gatgttgtag
     3061 aatgttgagt catggacgtc ctcctggact cttttctctc ctatctcgcg gtcgaaaaag
     3121 ggctttccga aaacacgctc gaatcctacg gaagggattt aaggaaattt atccgttcga
     3181 tggaggaagc gggtgtgcgc tcggccggag agatcaatta cagtcacata ctcgattacc
     3241 tctccctgtc gaaggaaaag ggcttcaacg cgacctcgat cgtgagaagc atcgtatcga
     3301 tcaggcagtt cttcaagtac ctgctgatgg aaaagatagt cgatgccgac cccgcctcgg
     3361 gtatcgggac tccgaagatg agaaaggggc ttccagtagt tatcacgctc gacgaggtgg
     3421 aggcccttat atccgcgcca gacgaatcga cgcccgaggg gctcagggac gcggccatgc
     3481 tcgaggcgct ttacgccaca gggataaggg tgtccgagct cgtggggcta aagctcaacg
     3541 acgtaaattt cgagctcggg ttcgtggtag tttacggcaa agggtccaag gaaaggattg
     3601 tcccaatggg ggacaaggcg aggcagaagc tcctcgaata cctgggtacg tcacggcccg
     3661 cgatgctcaa gggaagggaa gcgaaagcgc tcttcgtcac gaggctcggg agaggcatga
     3721 cgcgccaggg gttctggaag ataataaagc attactcgct caaagcgggt atagccagaa
     3781 agataagtcc ccataccctc aggcattcgt tcgcgacgca cctcctcgag cgcggcgccg
     3841 acctgagaac gatccagatc atgctcggac actcggacat atccacgacc cagatataca
     3901 cacacgtcga gagcgagaga ctcaaggtga tacacaaaaa atatcacccg agatcgtaaa
     3961 acaccccggg ttcaagtata ttactaagaa aaacggagat ttataacgac gatatgaatt
     4021 tcgatttcct ta
//
LOCUS       DASSJH010000384         4288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1500547, whole genome shotgun sequence.
ACCESSION   DASSJH010000384 DASSJH010000000
VERSION     DASSJH010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500547"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1074)
                     /locus_tag="VFJ67_08485"
     CDS             complement(259..1074)
                     /locus_tag="VFJ67_08485"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7290458.1"
                     /translation="METISILGCGWLGLSLAEFLLGKGYKVKGSTTARSGLDEMKSRR
                     IEPFYLVIDPGLRGEGLADFFDCDVLVVNFPPERRDDIAEYHPAQIRSLIAGLAGSRV
                     KKVLFVSSTSVYPDLDREVTEDESAQPSKQSGIALLRAERLLRDSGSFVTTIVRFGGL
                     IGYDRMPGRFLAGKKGIGGGDAPVNLIHRDDCVGIIYCIISQNLWGETLNACADEHPL
                     RKDYYTARALALGLEPPEFKEGGKGGYKIVNSGRLKKLLGYRFKYPNPSRIED"
     gene            complement(1151..2050)
                     /locus_tag="VFJ67_08490"
     CDS             complement(1151..2050)
                     /locus_tag="VFJ67_08490"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7290459.1"
                     /translation="MATKENMIVMRTDFEGVKPPSRGKVRDIYDLGDRLLLVASDRIS
                     AFDVVLPEGVPGKGRVLNTISEYWFGQTADIVPNHLISTDVDDYPAEFRKYREVLEGR
                     SMLVRKTRPLPVECIVRGYVAGSGWKEYRSSGSICGIPLPPGLLESSRLDKPIFTPST
                     KAEAGTHDENISFERAAGLLGRELAEKVSGVSIALYSRARDIAEKKGIIIADTKFEFG
                     LDEKSGELVLIDEALTPDSSRFWPMDEYRPGGPQKSFDKQFVRDYLESLDWDKKPPAP
                     SLPPDVVRRTSEKYKEALERLTG"
     gene            complement(2170..2466)
                     /locus_tag="VFJ67_08495"
     CDS             complement(2170..2466)
                     /locus_tag="VFJ67_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290460.1"
                     /translation="MDTPMLWRLVGAFINVTLLTRGAHLVLKRWVGDPLKRAYIVLIL
                     VAVIDFAGVWALYRDLATSFYVTLFYYVPLLLMWFLKDVLDASRARGKASGSAE"
     gene            2641..3513
                     /gene="htpX"
                     /locus_tag="VFJ67_08500"
     CDS             2641..3513
                     /gene="htpX"
                     /locus_tag="VFJ67_08500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET7290461.1"
                     /translation="MNTLKSLILLAVLSAILMWIGGAIGGKQGLVLAFGFAMVMNFAS
                     YWWSDKIVLSLYGAKEVKQEDAPELYGIVQELSSRAGLPMPRVYITPQEQPNAFATGR
                     NPEHAAVAVTQGILRLLSRDELTGVLAHELSHVKHRDILIGSIAATIATAITYLAYMA
                     QFAAIFGGGGGGGDRDRGGNPLGLLVMAIVAPLAATIIRLAISRTREYAADEGGAEIS
                     GNPISLANALRKLGMASDRIPLQVSEQVADSTSHMLIASPLSGGGLSSLFSTHPPMEE
                     RIRRLEEMAAGISR"
     gene            complement(3573..>4288)
                     /locus_tag="VFJ67_08505"
     CDS             complement(3573..>4288)
                     /locus_tag="VFJ67_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7290462.1"
                     /translation="KVSVLSGGEKSRVALAKTLLSPRNFLVLDEPTNHLDIQSRNVLI
                     EALRQYKGTFLVVSHDRHFLDRVTNRIWYAEKGGIVTYPGTYSEFRYHQNLYETAETE
                     GPDESGERGMNGRKSGVGGPQKSQDESGAGRRLEAEKRNLLYRELRERGIENMENWRE
                     LSGKQLRSALAELEQRINGIEKNISEVEAFLADPENFRDGRRSAEASIEFSRLSGELK
                     KLYDRWEAVSSHLETHASN"
BASE COUNT          931 a         1207 c         1157 g          993 t
ORIGIN      
        1 tcatatgtat taatatatgc attacccgtt tatttttata ggctgcgagt gaaggcgcag
       61 aagaaccggc gtcccgaata aagccggact ccggtgtttc attaaaagaa tatctattgg
      121 gtttctctat atttgttccc aaatatgaat gagggtgcaa aataggattt ctttcatttt
      181 taacagtgat tagtatagca taacgtccgt caaatccaac ccctcacata cgccgcaaac
      241 cccgggaaag gcggctgttc agtcctcgat ccgggatggg ttcgggtatt tgaaccggta
      301 tccgaggagc ttctttagcc tgccgctatt aactattttg taccccccct ttccgccttc
      361 tttaaattca ggtggttcga gcccgagcgc gagtgcccgg gccgtgtaat agtcctttcg
      421 gagagggtgc tcgtccgcgc acgcgttaag ggtctctccc cacaggtttt gactgatgat
      481 gcagtatatt atgcccacgc agtcatcccg atgaatgagg ttcacgggag cgtcgccccc
      541 tccgatgccc tttttcccgg cgaggaacct gcccggcata cggtcgtagc ctatgagccc
      601 gccgaacctg actattgtcg ttacgaaaga gcccgaatcc cggaggagcc tctcggccct
      661 aaggagcgct atgcccgact gttttgaggg ctgcgcgctt tcgtcttcag tgacctcgcg
      721 gtcaagatcg gggtaaaccg atgtcgagct gacaaagagg acttttttca cccgggagcc
      781 ggcgagcccg gcaatgagcg accttatctg cgccgggtgg tattcggcga tatcgtccct
      841 cctctcggga ggaaagttga cgacgagcac atcgcaatcg aaaaaatctg cgagcccctc
      901 tccccttagc ccggggtcga tcacgagata aaagggctcg atcctgcggg acttcatctc
      961 atcaagcccg gaccgtgcgg tggtcgagcc ttttaccttg tagcccttcc ctagcaaaaa
     1021 ttcggcgaga gacagtccga gccagccgca ccccagtatg cttatggttt ccatatcaac
     1081 ctgccagaag cgattgactt aggccgaact cgatgatgcg cgtctcgttc gaagctgcgg
     1141 tcaattccgc tcacccggta aggcgctcga gcgcctcttt gtatttctca gaagtcctgc
     1201 ggacgacgtc gggagggagg gacggcgcgg gtggcttttt gtcccaatcg agggactcga
     1261 gataatccct tacgaattgc ttgtcgaagc tcttttgcgg gcctccgggc cggtattcat
     1321 ccatgggcca gaacctcgaa gaatcgggcg taagggcttc gtctatgagg acaagctcac
     1381 ccgacttttc gtctaaaccg aattcgaact tggtgtctgc gatgattatc ccctttttct
     1441 ccgcgatatc ccttgctctc gaataaagag caatgctcac accgctgacc ttttccgcga
     1501 gctccctgcc caggagcccg gccgctctct cgaaactgat gttctcgtcg tgagtgccgg
     1561 cttcggcctt cgtggacggg gtgaatatgg gtttatcgag cctggacgac tcgaggagac
     1621 cgggcgggag cggtatgccg catatagagc cgctgctcct gtactccttc cagcccgagc
     1681 ccgcgacgta tccccttacg atgcactcga ccggaagggg ccttgtcttc ctcacgagca
     1741 tgctcctccc ttcgagcact tccctgtact tacggaactc agccgggtag tcgtcgacgt
     1801 cggtcgaaat aaggtggtta ggtacgatgt cggccgtctg cccgaaccag tactcggaaa
     1861 tggtattcag caccctcccc ttccccggta cgccctccgg gagcacgacg tcgaatgctg
     1921 aaatacggtc cgaagctacg agaaggagcc tgtccccgag gtcgtagata tcccttacct
     1981 tacccctgga aggaggcttc acgccctcaa aatccgtgcg catcacgatc atgttttctt
     2041 ttgtcgccat aagtcgcagg aagcagctaa gaaatcagcg gagagaattc tattaaagaa
     2101 aaaaagatat gtcaaaatgc tgcgatccgc atgaaatcag agaacttagt gagcggactg
     2161 aatataattt cattccgcgc ttcccgacgc cttccccctt gctctcgatg cgtcgagcac
     2221 gtccttaagg aaccacatga ggaggagcgg gacgtaataa aaaagcgtga cgtagaaaga
     2281 ggtggcgaga tcccggtaga gggcccagac cccggcgaaa tcgatgacag cgactaatat
     2341 cagtactatg tacgcccgct ttaaagggtc tccgacccac cttttcagaa cgagatgcgc
     2401 tcctctcgtg agaagcgtca cgttgataaa cgcgccgacg agcctccaca gcatgggagt
     2461 gtccatgtta ttcaggaccc ctcgttaaag tcccgcccgg cgaactttta cttgaatttt
     2521 cgacgcgaat cattattttc attatatcac cccgcgggca tggtgtaagt acgctacgtt
     2581 tacgggatgc atccggtttt attggataaa ttgagtttta attagtcggg agagataaaa
     2641 atgaatacac tcaagagtct catactgctc gcagtgcttt ccgcgatcct tatgtggatc
     2701 ggcggggcta tcggcgggaa gcaaggcctc gtattagctt tcggattcgc gatggtaatg
     2761 aatttcgcaa gctactggtg gagcgacaag atcgtccttt cactctacgg ggcaaaggag
     2821 gtcaagcaag aggacgctcc ggagctctac ggcatcgtac aggagctttc ctcgagggcc
     2881 ggactcccga tgccgagggt ttatataacc ccgcaggagc agccgaacgc gttcgccacg
     2941 gggaggaacc ccgagcacgc agccgttgcc gtaacgcagg gcatattgag gctcctcagc
     3001 agagacgagc taacaggggt gctggctcat gagctctcgc acgtaaagca cagggacata
     3061 ctcatcgggt cgatagcagc gaccatagcg accgctatca catacctcgc ctacatggcc
     3121 caatttgccg ccatattcgg cggaggagga ggcggaggcg acagggacag gggagggaac
     3181 cctctagggc tcctcgtgat ggcgattgtc gctcctttgg cggccacaat tataaggctc
     3241 gcaatctcga ggacgaggga atacgccgcc gatgagggcg gggccgagat aagcggcaac
     3301 ccgatatccc tcgcgaacgc cttgagaaaa ctcggaatgg cttcagacag gataccgctc
     3361 caggtgagcg aacaggtcgc cgacagcacg tctcacatgc tcatcgcgag cccgctctcg
     3421 ggcggagggc tgtcgtcact tttcagtacc catcctccga tggaagagag gatcagaagg
     3481 ctcgaagaga tggctgccgg aatttccaga taaataatat tcttcatccg aaccgcgcat
     3541 agactttcgc cgaatgccgt aatggagagt tgctaattgg atgcgtgagt ttcgagatgc
     3601 gaggaaacgg cttcccacct gtcgtacaat tttttaagtt cgccgctcag tcggctgaat
     3661 tctatcgagg cctcagccga gcgcctgccg tccctgaaat tttcgggatc ggccaggaaa
     3721 gcctcgacct cggatatatt tttttcgatg ccgttgatcc tctgctcgag ctcggcgagg
     3781 gctgagcgga gctgcttccc ggaaagctcg cgccagttct ccatgttctc gatcccgcgc
     3841 tcgcggagct ccctgtagag caggttccgc ttttcggcct cgagcctcct gccggcgccc
     3901 gattcgtcct gactcttttg aggcccgccg accccggatt tcctcccgtt catcccacgc
     3961 tcgcccgatt cgtccgggcc ttcggtttcc gcagtctcat ataaattttg atggtatctg
     4021 aactccgaat acgtgcccgg atacgtgacg attccccctt tctcggcata ccatatcctg
     4081 ttcgtcacac ggtcgaggaa atgcctgtca tggcttacga cgaggaatgt ccccttatac
     4141 tgcctgagtg cctctatgag cacgttcctc gactggatgt ccaagtggtt tgtaggctcg
     4201 tcgagtacga ggaagttacg gggagagagg agtgttttgg caagcgcgac acggctcttc
     4261 tcccctcccg acagaaccga tacttttt
//
LOCUS       DASSJH010000385         3064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1061265, whole genome shotgun sequence.
ACCESSION   DASSJH010000385 DASSJH010000000
VERSION     DASSJH010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061265"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1111
                     /locus_tag="VFJ67_08510"
     CDS             2..1111
                     /locus_tag="VFJ67_08510"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HET7290463.1"
                     /translation="MDAKKSEMRAKGVDIIDLSVGDPDIPTPPHIVEALKRAAEDPQN
                     HRYPSYEGLLSFRTAVSDWYMKRFGVELDPKTEVVALIGSKEGIAHTPLAFIDPGDIG
                     LYTDPGYPVYPTSIGFAGGVPYPVPLLKENGFLPDLGSIPEEIARKARIMFVNYPNNP
                     TAAVAGAEFYKDLVDFAVKYNILICHDAAYTEIAYEGYKPSSFLEYDGAREVGIEFHS
                     LSKTFNMTGWRIGFAVGNKKAIGGLGKIKTNIDSGAFQAVQIAGVEALKNYETGLADR
                     IEIFQERRDIFCKGLDEAGLSYSKPMATFYVWFGVPSGLSSKDFSSKLLTETGIVVTP
                     GTGFGKYGEGYVRVSTTFSTERIVQAVERLKEAKF"
     gene            1148..2689
                     /locus_tag="VFJ67_08515"
     CDS             1148..2689
                     /locus_tag="VFJ67_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HET7290464.1"
                     /translation="MEYFILASKIIITVLYAVTLTLLCIFGLHRYYLSYLYKKNKNKK
                     PLPASRYEKLPVVTIQLPIYNEMYVVERLIASVCKIDYPRDLLEIQVLDDSTDRTVEI
                     ASECVERFRGSGTDIVYIHRENREGYKAGALREGLKRAKGELLAVFDADFIPPAEFLR
                     NTVDFFTDPGVGMVQVRWGHVNLDYSTLTKAQSILLDGHFMIEQTSRFNGGMFFNFNG
                     TAGVLRKECIESSGGWQHDTLTEDLDLSYRAQLAGWKLVYLRDMIADAELPVDMNAFK
                     SQQHRWAKGGVQTALKTLPEIFRRDNLPFKVKSEAFFHLFGNISYLLLLILFLLMFPM
                     GFFWTELGWDSGIVVNVFAITAGTLSFVLFYIITVREVHGKKWLSYLKYVPLAISMGA
                     GMAINNSKAVLEALLGKNSEFRRTPKFAVTSRNDNWRSRGYIASKEATAAVEIIFGVL
                     FFIQTVLAVYMGYLGWVPFLILIQFGFLYTGLLSIFHGSGKKGADPGIGTIIPEPALQ
                     RDKDF"
     gene            2869..>3064
                     /locus_tag="VFJ67_08520"
     CDS             2869..>3064
                     /locus_tag="VFJ67_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7290465.1"
                     /translation="MKICTLASGSSGNCLYVESGETRILVDAGISLRQIVLKLRKLDV
                     DLTDIDAVVVTHEHSDHTAAL"
BASE COUNT          741 a          741 c          850 g          732 t
ORIGIN      
        1 aattgacgct aagaagagtg aaatgagggc taaaggggtg gatattatcg atttaagcgt
       61 cggcgatccc gatataccca cgcccccgca tatagtcgag gctctgaaaa gagccgccga
      121 ggacccgcaa aaccacagat acccctcata cgaaggcctc ctttcgttca ggacggctgt
      181 ttcggactgg tacatgaagc ggttcggtgt ggagctcgac cccaagaccg aagtcgtggc
      241 gctcataggc tctaaagagg ggatagcgca cactcctctc gcattcatcg accccggtga
      301 catagggctg tatacggacc cgggttatcc tgtttatccg acttcaatag gcttcgccgg
      361 cggtgttccg taccccgtac cgctccttaa agagaacggt tttctccccg acctggggtc
      421 tataccggaa gaaatcgcca ggaaagcaag gataatgttc gttaactatc cgaacaaccc
      481 gactgctgcc gtggcgggcg ccgagtttta caaggatctt gtggattttg ctgtcaaata
      541 taatattctt atatgtcacg acgccgccta taccgagatt gcgtatgagg gatataaacc
      601 gtccagtttt cttgagtacg acggcgcccg ggaagtcggg atcgagttcc attccctttc
      661 caagacgttc aacatgaccg gttggaggat cggttttgct gtcgggaaca aaaaggcgat
      721 cggaggactc ggcaagatta agacgaacat agattcggga gcttttcagg ccgttcagat
      781 cgccggtgtg gaagccctga aaaattacga aaccggtctc gcggacagaa tagagatatt
      841 ccaggagcgg agggatatct tctgcaaggg actcgacgaa gccggtttga gctacagtaa
      901 acccatggcc actttttacg tatggttcgg agtcccgtca ggtttgtcgt caaaggattt
      961 ctcgtcaaag ctcctgaccg agacgggtat agtcgtgacc ccgggcacgg ggttcggaaa
     1021 gtacggcgaa ggctatgtgc gggtctcgac cacattcagc accgagagaa tagttcaggc
     1081 tgtagagagg ctcaaagagg caaagttctg aaactgtccg gaatgatgag gcagcgggaa
     1141 atgtgaagtg gaatatttta ttttagcttc taagataata atcacagtcc tatatgccgt
     1201 aacgctgaca cttctctgca tattcggtct gcaccggtat tacctttcct atttatataa
     1261 gaagaataag aataagaagc cgctgcccgc gtccaggtac gagaaactgc cggtagttac
     1321 gattcagctc cccatttaca atgaaatgta cgtagtggaa aggcttattg cctcggtctg
     1381 caaaatcgac tacccgaggg acctgctcga gatacaggtg ctcgacgact cgacggacag
     1441 gaccgtggag atcgcgagcg agtgcgtgga gcggttcaga ggatcaggga cggacatcgt
     1501 atatatacat agagagaaca gggaagggta caaggcggga gcgctcaggg agggtcttaa
     1561 aagggccaag ggcgagcttc tggctgtttt cgacgccgac ttcataccac cggcggaatt
     1621 cctccggaat acggtggact ttttcaccga tccgggggtc ggaatggtcc aggtgaggtg
     1681 gggtcacgtg aacctcgatt actctaccct tacgaaggcc cagtcgatcc tcctcgacgg
     1741 gcatttcatg atagagcaga cgtccagatt taacgggggg atgttcttta acttcaacgg
     1801 gaccgctggc gtgctgagga aggagtgcat tgagtcttcc ggcggatggc agcacgatac
     1861 gcttaccgag gacctcgacc tgagctacag ggcccagctc gccggctgga aactggttta
     1921 tctccgcgac atgatcgccg atgccgagct gccggtggat atgaacgcct ttaaatcgca
     1981 gcagcacagg tgggccaaag gcggggtcca gactgcattg aagacactcc ccgaaatctt
     2041 caggcgcgat aaccttccct tcaaggtcaa gtccgaggcg ttctttcatc tctttggcaa
     2101 tatttcgtat ctgctccttc tcatcttatt tctgctcatg ttcccgatgg gttttttctg
     2161 gaccgagctg ggctgggaca gcggtatcgt cgtaaacgtg tttgcaataa ctgcgggcac
     2221 cctcagcttc gtgctcttct atataatcac ggtcagggag gtgcacggga agaaatggct
     2281 ctcttatctg aagtatgtgc ctctcgcgat atctatggga gccggtatgg cgatcaataa
     2341 ctcaaaggcc gtgcttgagg ctcttttggg caagaactcg gaattcagac gcactccgaa
     2401 attcgccgtt acatcaagga acgataactg gcgttcgaga ggatacatcg cgtcaaaaga
     2461 agcgactgcc gccgtagaaa taatattcgg cgtattgttt tttattcaga ccgtgctcgc
     2521 tgtttatatg ggctatctgg gctgggtgcc gtttctgata cttatccagt tcgggttttt
     2581 gtatacgggg ctgctttcta tttttcacgg ctcgggcaaa aagggcgccg atcccgggat
     2641 cggcacgata atccccgagc ctgctcttca gagggataag gatttttaac ggggacgata
     2701 agagagcata cacccgtccc ccgctgccga tagagtagca gcttttttat tcatgtgtcg
     2761 tggatttata tattctgttc tgttcctccg gataatggtg ttgacttata ggatattcgt
     2821 aatccatatt aaatttccaa aacggattaa acagtctgcg gccgacgaat gaagatatgc
     2881 acgcttgcaa gcggcagttc gggaaactgc ctttacgtgg agtccgggga gacgaggata
     2941 ctggtcgatg cgggtatcag cctgaggcag atcgtcctca agctgaggaa actcgacgtg
     3001 gacctgaccg atatagacgc ggtagtcgtg acgcacgaac attcagacca tactgccgcc
     3061 ctca
//
LOCUS       DASSJH010000386        13535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_419085, whole genome shotgun sequence.
ACCESSION   DASSJH010000386 DASSJH010000000
VERSION     DASSJH010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419085"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFJ67_08525"
     CDS             <1..1060
                     /locus_tag="VFJ67_08525"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HET7290466.1"
                     /translation="RSGNWEELSRKVDESKTSWLVAGISSPLDTAHPLPERPRGYTAV
                     SSDGSQIFPDRHEALPCYLINISSIALKYGDNAGAKLDSGPSLFYRDEDRYTTWGGKK
                     VPADSEVISVKRTLMEFERILELVKGSGSHANTIALTDGMLILWRLEAAPPDFRDAVM
                     IPFLRIMDEFRSLRVPVAGYISYPGSADVINALRVGLCPERTSFCNQCPYTDLPELPC
                     SPIEGLTDRFLFSTVLDTGEASPVFRSSSRILEMYGGHRVYFFYINIGEEIARVEIPE
                     WVALDSELLSLVHTLVFDQAKKGKGYPVAIAEAHEQAVVKAKDRDFFFSLVREAMVRS
                     DFAVTVSRKGLSKRVPGV"
     gene            1057..1626
                     /locus_tag="VFJ67_08530"
     CDS             1057..1626
                     /locus_tag="VFJ67_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290467.1"
                     /translation="MSDYIGEVVESGTKGFVARTRRVGEAPHFGSFVRTDASSPVYGL
                     VYEIITGSREPGRKPDAYDMSIEELRREQPQIFELLKTEFHVLTLGYLDAGKIRFALS
                     PLPPPIHSFVYECTDREKEALSSEDFFLRAIISSPNVPVDDLIISSLLSAQEVRKNDR
                     DFMVRMGKSLSRYLKDDYERLSSILRRLL"
     gene            complement(1636..2313)
                     /locus_tag="VFJ67_08535"
     CDS             complement(1636..2313)
                     /locus_tag="VFJ67_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7290468.1"
                     /translation="MKLLLFDIDGTILLTNGAGTRAANRAFEKIYGHAGAMDGVDAAG
                     KTDPLILREMFGNTLSRSYAHDEAEKLFEEYVIFLEEEVENSPIDIMPGIPYLLDNLS
                     LRKDILLGIATGNIERGARIKLERAGLDGHFRAGGYGSDSWKREELIRIAIGRAKNLL
                     TDGDDFEKIYVIGDTPHDIVHGRATGAVTVAVATGRYSAAELSKHEPDYVFEHLADYE
                     SVIGIFG"
     gene            complement(2325..3323)
                     /locus_tag="VFJ67_08540"
     CDS             complement(2325..3323)
                     /locus_tag="VFJ67_08540"
                     /inference="COORDINATES: protein motif:HMM:NF027495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HET7290469.1"
                     /translation="MKKLIGGWVHIPGIHCGSVTLRDVMTYYGYPWSEAMCFGIGAGL
                     GFYYSVREDISPTRMIFVRGPGMEPAFFSLAIGPVSWKHADEDALGVIKGAVDRDVPA
                     IIQTDIYYLDYYKSSTHFPGHIVSVWGYDDESREMLVADTHFDGLQKVPYDEFIEGMG
                     SRSLPNPLDYNYMDINPDGKVRPLPEIIPEALRLNAVKMIEGRTSARGESGVSLIRSW
                     SEDLPSWTDAPDWKWCARFGYQVIEKRGVGGGGFRWIYRDFLKEAEDIIPELRDLNLS
                     IRMNHIGNRWHEIAILLKEISEKEHPDENLLRSASEKAEVLWELERDFYVTVLENV"
     gene            complement(3890..4324)
                     /locus_tag="VFJ67_08545"
     CDS             complement(3890..4324)
                     /locus_tag="VFJ67_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7290470.1"
                     /translation="MRIKVWEPFRSFRPFYGDLDKWFDEAANANSPANGTWHPAVDVY
                     ETEGSYVLKAELPGVGKEDIKIDVNNNALTIKGEKKFEEKTEKDNYMRVERSYGSFTR
                     TFALSDKVDSGNIKAAFKDGVLEITLPKKEEAKPKEIKVEIN"
     gene            4463..4969
                     /locus_tag="VFJ67_08550"
     CDS             4463..4969
                     /locus_tag="VFJ67_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7290471.1"
                     /translation="MLTAERLIKLYNMKPLPGEGGYYVETYRASESIEGSSLPERYGS
                     DRSLGTAIFYLLTPDTRSLIHRVKSDEIYHFYLGDPVQLVLIHPSGTIKVIFLGQDIR
                     AGQFVQAVVPAGIWQGSFLLEGGQFALMGATVAPGFEFGDTELGRRDELLEQYPQHRD
                     LIASLTEG"
     gene            complement(4966..5997)
                     /locus_tag="VFJ67_08555"
     CDS             complement(4966..5997)
                     /locus_tag="VFJ67_08555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7290472.1"
                     /translation="MNRTKNVFITVLCVIASLGASIAAAGARETIDRVVAIVNEDIIT
                     LSELRELALSINPTSTEAIDENAVLQQMIEQKLLEQQADKMGIKVTEAELDASIKQVQ
                     QKFGLNDQQMQEVLKKQNLTPESFREQWRLQTLSNKLLESQLKNKIVITEEEIQDYYV
                     KNYGGGGTATESEETVSVDTQGGGEEVKIAHILISANTPDAEKKAEEVAEMAKSGKDF
                     GELAKEYSEDKLSADKGGDLGYFKKGDLIETLEVAVDSTPVGGITGPVESPAGYHVIK
                     VLERTGAEKAEKDDKDKDSKKDSGKVMAIDEDTRKEITEILYKQKAEAQLKTWLDKIK
                     QEAYIEVRL"
     gene            complement(5990..6880)
                     /locus_tag="VFJ67_08560"
     CDS             complement(5990..6880)
                     /locus_tag="VFJ67_08560"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7290473.1"
                     /translation="MKYIVVIAAAVLLAGCSDFNKFFESRREVPTEKEAGPEVIALVG
                     GDEITKAELMRALERMPYKQKKLYQSSPEKLGEFLDIYINQKILYAEAVKRGIDKRED
                     VIEKTENFKKQLVGKTFGQEVLKSIRVSGSEIDDYYRRHKEDYEQINVSEIYIKADPG
                     NGTTKEAALARAREAYEKAKAGGDWDELVKLYSDDETTREKGGGRGYVQRGEFGPVID
                     GQIFGMKKGEITGPLEVSGGYFIIKINEGAAPIPEGQLKRRVESQIINEKLVEYVNGL
                     RNEARVEVYRDRLEESLTSE"
     gene            complement(6887..10333)
                     /gene="mfd"
                     /locus_tag="VFJ67_08565"
     CDS             complement(6887..10333)
                     /gene="mfd"
                     /locus_tag="VFJ67_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET7290474.1"
                     /translation="MKDRPRIKLDNAIKTLNESGKFRNLLRKIESGEKLIPLSGLGGS
                     SKFFLVYALRETTRRPILVLSPTAKRADTASHDLSFFLGERPRVLLRKEIGTGRPVFS
                     TAAAKSGSDRIAWLNSAINGEVLVAESQALFDKTIPRDVFSKSVIKVGKGLLSFRDEL
                     ISGLVKSGYTLTDFVQNEGDISRRGSIVDVFSPGSGHPVRLEYIGDEIGSIRRFNLES
                     QKSTDRVESAELLPASEIIMEPEPLGRAVEYLKKRAGESGVTAREKLPLIEDIESGIR
                     VQNIEWLLPAFYPEPGSALDFLPEDALIITEDAEDSPKSVERWRESLGLTKNYLTKNI
                     KIAPGMDELYFTEEEVGESSGRFRNIMIHDLEYAEGRMDGIRFGGEPASIEVRKESES
                     PLDAIDERIYEARQEGFTLLFVFRTHTELEKLLMLLRERGITKINAHTGELSHGFKLS
                     EGKFEIVTEGDIFGEKRERVSRGRGADVPGAFITSFSELKPGDYIVHVDFGIGIFRSL
                     KRLRIGDSEGDFIQCEYAGGDKVYVPIDRLKLVQRYIGEGKSTKVDRLGQESWKKRVR
                     RVRAAVENVARELLELYARRKALKGYGYLPRGEMFREFELGFSYEETPDQEAAIEEVM
                     NDMEAPRPMDRLICGDVGFGKTEVALRAAFRAVTDGKQVAFLVPTTLLAEQHYKTAVS
                     RLRGYPVVVDALSRFRTGKEESGIRSRLEDGKIDIIIGTHKLLGEKIRFKDLGLVIID
                     EEHRFGVSQKEKLRKIKEGVDTISLSATPIPRTLQLSLAKIRDISLINTPPEGRQTIE
                     TYIHHSSPAVIEEAITRELDRGGCVFFIHNRIEDIYRVADRVRKLFPEAKIEVTHGRM
                     RERDLERSISKFIEGGVDILVTTAIVESGLDIPRANTIIIDDAHTFGLADLYQLRGRV
                     GRSDKKAYAYFLVPGTESLTLDARRRLKAISEFRELGSGFKLALSDLEIRGAGQLFGV
                     EQSGHIADVGLELYLDMLEGAVRRLEGKVPLEEPEPDISASTPAFIPDEYISNDTERL
                     LVYKRLSYITTEEGLREITGELRDRFGEIPPPAHSLIDIIGLKIMMKKLGIEKADIGS
                     KMAAFSFSPKSGLYERFKPQGRMEVYFEKRDPLGETKKILENLAAPGARETGQKDMTR
                     RR"
     gene            complement(10359..11438)
                     /gene="leuB"
                     /locus_tag="VFJ67_08570"
     CDS             complement(10359..11438)
                     /gene="leuB"
                     /locus_tag="VFJ67_08570"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET7290475.1"
                     /translation="MRLPRVLVLPGDGIGVEVTDVSLNILDILGKKHNIDFEFEKELF
                     GGSSIDAHGVPITEEVLEKARASDAVLMGAVGGPKWENLEHSKKPEAGLLALRKELDA
                     FANLRPAVVYPALADASSLKREIVEGADIMVVRELTGGIYFGTPRGIDKLPGGGEKGY
                     NTLVYTTREIERIARVAFDIARKRKKKVTSIDKANVLEVMQLWRTVVTRVREAEYPDI
                     ELEHLYVDNAAMQLIRRPRSFDVMLAGNLFGDIISDEAAQLTGSLGMLPSASIGKGAI
                     YEPVHGSAPDIAGQDIANPIASLLTSAMMLKYTFNRDGASDDIENAVKTVLEKGYRTA
                     DIYQEGTKKVGCREMGALIAGELKG"
     gene            11621..13378
                     /locus_tag="VFJ67_08575"
     CDS             11621..13378
                     /locus_tag="VFJ67_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290476.1"
                     /translation="MSFIRRKWLVSLLLFLVSLGVYSPSLRNGFVWDDVETIEQSYYI
                     YNASSMGAMFIPPEKESKDALYYRPLIYASMVLDKALWGITPFGFHLSNIVLNAFSTV
                     CAYWFLLLLLGVFGCERRCGAAFIAGFLFAFYPMHVESVSWVSGRSDVLCALFFFLAL
                     IFHILSLRKTGFLVLTAAFFALSLSSKEVAVVFPVTALAFDLINRSKGRKANILRYVV
                     YASVLALFFYLRGRTYVNFPELSSVRVPGGTPGHGDVIPAPGILGAVKVVLVSYMYYF
                     TKLVFPYTFNAFTAKAPGGPAYVISSVMTFGFLLLGVFYSIKKRYNVSAFALIWIPVT
                     LLPSVLIALTDIPSASVAERYLYIPSAGFCLFLAWIFTRALVSVRRDRILLAAAFILT
                     ASYLFFTVDRQRVWRDGVSLWGDTVGKSPGSAIPHINYGLALLDEGKDDEALKELLRN
                     FDEGVEIDDRGRSVTASNIGVVYINKKDVRNAEKWFLKAYGYNPGYYKTSYHLGLVLY
                     LRGKSGGSREDYVSALGYLNKSLQMRPFYGKAYLLQAMVYGELGERDKARESAERALE
                     SGLTDKLAAKARKIISAPD"
BASE COUNT         2590 a         4046 c         3419 g         3480 t
ORIGIN      
        1 caggtccggg aactgggagg agctctcccg gaaggtcgat gagagcaaga cctcctggct
       61 cgtagcgggt atctcctctc ccctcgatac tgcgcacccc ctgccggagc gcccccgcgg
      121 ctacacggcc gtatcctcgg acggctcgca gatattcccc gaccggcacg aggcgctccc
      181 gtgctatctc atcaacataa gctctatcgc gcttaaatac ggggacaacg ccggggcgaa
      241 gctcgactcg ggcccttctc ttttctacag ggacgaggac aggtacacga cgtggggagg
      301 gaaaaaggtc cccgccgaca gcgaggtcat atcggtaaaa agaacgctca tggagttcga
      361 gcggatactc gagcttgtca aagggagtgg atctcatgca aacacgatcg cccttacgga
      421 cgggatgctc attctatgga ggctcgaggc ggccccgccc gacttcaggg acgccgtgat
      481 gatcccgttc ctccgcataa tggacgaatt cagatcactc cgcgtgcccg tggccgggta
      541 tataagctat cccgggagcg ccgacgtcat aaacgcgctc agggtcgggc tctgccccga
      601 gagaacgagc ttctgcaacc agtgcccata tacagacctc ccggagctcc cctgctcccc
      661 gatcgaaggg cttacggaca ggttcctctt ctcgaccgtg ctcgatacgg gcgaggcttc
      721 tcccgtattc aggagctcgt caaggatact ggagatgtac ggcggacatc gtgtttactt
      781 tttctatatc aatatcggcg aggagattgc gagggtcgag atacccgagt gggtcgccct
      841 cgacagcgag ctcctttcgc tcgtccacac gctggtgttc gatcaggcga agaaagggaa
      901 gggctatccg gttgcgattg cagaggcgca cgagcaggcg gtcgtgaagg cgaaggacag
      961 ggattttttc ttctcgctcg taagggaggc gatggtgagg tccgactttg cggtaaccgt
     1021 atcgagaaag ggattgtcga agagggttcc gggcgtatga gcgattacat aggcgaagtg
     1081 gtcgagtccg ggacgaaggg tttcgtcgcg cgcacccgca gggtggggga agcccctcac
     1141 ttcgggagct tcgtaaggac tgacgcgtcc tcgcccgttt acggcctcgt gtacgaaata
     1201 atcaccggga gcagggagcc cggcaggaag cccgacgcct acgatatgag catcgaggag
     1261 ctaaggaggg agcagcccca gatattcgag ctgctcaaga ccgagttcca cgtgctcacg
     1321 ctcggctatc tcgatgccgg gaaaatacgg tttgcgctct cgcccctacc cccgcccata
     1381 cattcgttcg tctacgagtg tactgaccgg gaaaaagagg ctctcagctc cgaggacttc
     1441 ttcctgagag cgataatatc ctcgccgaac gtgcccgtgg acgacctcat catatcttcg
     1501 cttctgagcg cgcaggaagt caggaagaac gacagggact tcatggtcag gatgggaaag
     1561 agtctctcga ggtatcttaa agacgactac gagaggcttt cttccatatt gcgccgcctc
     1621 ctgtaaacac gggggtcagc cgaatatccc gatcacgctt tcatagtcgg caaggtgttc
     1681 gaaaacgtag tccggctcgt gcttacttag ctctgccgcc gaatacctgc ccgttgcgac
     1741 cgctaccgtc acggcccctg tcgcccgccc gtgtacgatg tcgtggggag tgtcgcctat
     1801 aacgtatatt ttctcgaaat cgtctccgtc tgttaaaaga tttttcgcgc gtccgatcgc
     1861 aatccttatc agctcttccc tcttccacga atccgagccg tacccgcctg ctctgaaatg
     1921 cccgtcgaga cccgcgcgct cgagctttat acgggcgccc cgctcgatgt ttcctgtggc
     1981 gatgccgagc aggatgtcct tcctcaacga caggttatcg aggagatacg gtatgccggg
     2041 cataatatcg ataggagaat tttctacctc ctcttcgaga aaaataacgt attcctcaaa
     2101 caacttctcc gcttcgtcat gcgcgtagct cctcgaaagt gtgtttccga acatctccct
     2161 cagtatcagc ggatcggttt tccccgccgc gtcaacgccg tccatcgcgc ctgcatgccc
     2221 gtaaattttt tcgaacgccc tgttggcggc cctcgtcccc gcgccgttcg tgagcagtat
     2281 tgtcccgtct atgtcaaaga ggaggagttt catgggttat cgtcttaaac gttttccaga
     2341 acggtcacgt agaaatcccg ttcgagctcc cagagcacct cggctttttc tgacgcgctc
     2401 ctcaggaggt tctcgtccgg gtgctccttt tcgctgattt ccttaagtaa aattgcgatc
     2461 tcgtgccacc tgttgccaat atggttcatc ctgatagaca ggttcaaatc gcgcagctcc
     2521 gggataatat cttccgcttc tttgaggaag tccctgtaga tccacctgaa tccgccgccg
     2581 cccacacccc ttttctcgat cacctggtat ccgaacctcg cgcaccattt ccagtcagga
     2641 gcgtcggtcc acgacggaag gtcctcggac cacgacctga tcaggctcac gcccgattcc
     2701 cctcttgcgc tggtccggcc ctcgatcatc ttgacggcgt tcaaacgaag ggcttccggg
     2761 atgatctcgg gcagcggcct cacctttccg tcggggttta tatccatata gttgtagtcg
     2821 aggggattgg gaaggctcct cgatcccata ccttcgataa actcgtcgta agggaccttc
     2881 tgcagcccat cgaaatgtgt atccgcgacc agcatctcgc ggctctcgtc gtcataaccc
     2941 caaacggaca cgatgtgccc cgggaagtgt gtcgatgact tatagtaatc gagatagtaa
     3001 atgtcggtct ggattattgc gggtacgtct ctatcgacgg cgcctttaat gacaccgagc
     3061 gcgtcctcat cggcgtgttt ccatgatacg ggtccgatag caaggctgaa gaatgcaggc
     3121 tccatgccgg gccctctcac gaatatcatt cgtgtcgggc tgatgtcctc tctgaccgaa
     3181 taataaaacc cgagacccgc acctatgccg aagcacatcg cttctgacca cgggtatccg
     3241 taatacgtca taacgtccct gagtgtcacc gagccgcagt gtattcccgg tatatggacc
     3301 cagccgccga ttaacttttt cacagggata taagctacca taagatgttc tttagcgccc
     3361 cggactatga ccgggccgaa accgcgttac ttcacacaga aatatttttc gactttagac
     3421 ataatacaaa agtattaatc ccgcacctat atataaaaaa agaaataccc aaaatcagta
     3481 ctatcgtcct ctatataatc gatctaaata atattatatt aagttcagga tttggttccc
     3541 ggtgatggta ggggagagta gttcggatta ataatttcct gtagcgttta gaagagtccg
     3601 cggattcttc gtttttgaaa gagatttaac aagccatcat cctccatagg gtgagtcggc
     3661 gaaacccgtt ttcgctggct tacccttgtt ttttaaggac gttattctgc ttgctcccgc
     3721 tctgcggctt ctgaagctac cgacattcgg aaaagaacct cgaacgggca tgtaaaccga
     3781 taagccggtc acacatgcct gtgaccgccg ctcgaagggt ctgagtgagt cgattatcca
     3841 aaaaagataa aagcggcccc ccccgcagag agagccgctt tctttagaat cagttgattt
     3901 ctaccttaat ctctttgggc tttgcttctt ctttcttggg aagggttatt tcgagcacgc
     3961 cgtccttgaa ggcggctttt atgtttccgc tgtccacctt gtccgagagg gcgaaagtcc
     4021 tcgtaaagct cccgtaactg cgctctaccc tcatataatt atctttttcc gtcttttcct
     4081 cgaacttctt ctcgcctttg atcgtcagcg cgttgttgtt gacatcgatc ttgatgtcct
     4141 ctttgccgac gcccggaagc tcggctttca aaacgtaact gccttcggtt tcatatacat
     4201 ccacggccgg atgccaggtc ccgtttgccg gcgaatttgc gttagccgcc tcgtcgaacc
     4261 acttatcgag atccccgtaa aaaggtctaa agctcctgaa cggttcccaa acttttattc
     4321 tcatttgaat gcctccgtta tcgctggttt ttaaatgctt actcaaaaaa tattcacggt
     4381 tccccgttag tcaaggacgg tttttattga aaatcgaaaa atacggttaa gatattggct
     4441 cgcagccaaa ggagggaaaa tcatgctgac cgccgaaaga ctgataaagc tttacaatat
     4501 gaagccgctg cccggggaag gcggctatta cgtggaaacc taccgcgcct ctgagagtat
     4561 cgagggctcg tcccttcccg agcgctacgg ctccgacagg tctctcggca cggcgatatt
     4621 ctacctcctt acgcccgaca cccggtccct catccacagg gtgaagagcg acgagatata
     4681 ccacttctat ctgggcgacc ccgtccagct cgttctcata cacccgagcg ggactatcaa
     4741 ggtcatattc ctgggtcagg acataagggc gggacagttc gtgcaggcgg tggttcctgc
     4801 ggggatatgg cagggctcgt tcctgctcga aggcgggcag ttcgccctga tgggtgccac
     4861 ggtagcgccc ggcttcgaat tcggggacac cgagctcgga aggcgcgatg agcttctgga
     4921 acagtaccct cagcacaggg acttaatagc gtctctcacc gagggctaga gcctaacctc
     4981 tatatacgcc tcctgcttga tcttgtcgag ccaggtttta agctgcgcct cggctttttg
     5041 cttgtagagt atttcggtga tttcctttct ggtatcttcg tctattgcca tcaccttgcc
     5101 cgaatccttc tttgaatcct tgtctttatc atctttctca gctttttcag cgccggtcct
     5161 ttcgaggacc ttgattacat gatatccggc gggcgattcg acggggccgg ttatccctcc
     5221 gacgggcgtg ctgtccaccg cgacttcgag tgtctcgatg aggtcgcctt ttttgaagta
     5281 gccgaggtct ccgcccttgt cggccgagag cttgtcctcg gagtattcct ttgcgagttc
     5341 cccgaagtcc ttgcccgatt tagccatctc ggccacttcc tcggcctttt tttcggcgtc
     5401 gggggtgttg gcggatataa ggatatgcgc gatcttgacc tcctcacccc ctccctgtgt
     5461 atccacgctt accgtttctt cgctctcggt cgcggttccg ccgccgccgt aattctttac
     5521 gtagtaatcc tgtatctctt cctcggttat gactatttta ttcttcagct gtgattcgag
     5581 gagcttgttg ctgagggtct ggagcctcca ctgctccctg aagctctcgg gggtgaggtt
     5641 ttgcttcttc agcacctcct gcatctgctg gtcgtttaag ccgaatttct gctgcacctg
     5701 cttgatgctc gcgtcgagct ccgcctcggt gacttttatt cccatcttgt cggcctgctg
     5761 ctcaagcagc ttctgctcta tcatctgctg aagcaccgcg ttctcatcta tagcctcggt
     5821 cgaagtgggg tttatgctga gcgcaagctc cctcagctcg gagagggtta taatgtcctc
     5881 attcactatc gccaccaccc tgtcgatcgt ctctctcgcg cccgccgcgg ctatcgacgc
     5941 cccgagcgag gcgattacac ataaaaccgt tatgaataca tttttcgtcc tattcacttg
     6001 ttaagctctc ctctaatctg tctctgtaaa cctcgaccct cgcttcgttc ctcaggccgt
     6061 taacatactc gaccagcttt tcgtttatga tttgagattc taccctgcgt tttaactgac
     6121 cctccgggat gggggccgcc ccttcgttta ttttaattat aaagtatccc ccgctaactt
     6181 ccagcgggcc tgttatttcc ccctttttca tcccgaatat ctgcccgtct atgacaggac
     6241 cgaattcccc cctctgcaca taccctctcc cgcccccttt ctccctggtc gtctcgtcgt
     6301 ccgaatacag cttcaccagc tcatcccagt cccctcccgc cttcgctttc tcataagcct
     6361 cccgggccct ggcgagagcg gcctctttcg tcgttccgtt cccgggatcg gcctttatgt
     6421 agatttcgct cacgtttatc tgctcgtaat cctctttatg ccgcctgtaa tagtcgtcga
     6481 tctcgctccc gctcactctg atgcttttca atacctcctg gccgaacgtt ttgccgacga
     6541 gctgcttttt gaagttctcc gtcttctcta tgacgtcctc cctcttgtct atacccctct
     6601 tcacggcctc ggcgtaaagg atcttctggt ttatgtagat gtccaggaac tcgcctagct
     6661 tttcgggcga agattgatag agcttcttct gtttatatgg catcctctcg agcgcgcgca
     6721 tgagttcggc cttcgtgatc tcgtcaccgc cgactaaggc gattacctcc ggtcccgctt
     6781 ccttctccgt cggcacttcc cgtctcgatt caaagaattt attaaaatcg ctgcagcccg
     6841 cgagcagcac agcggccgca attacgacaa tatatttcat tcgctgtcat ctcctccgtg
     6901 tcatatcttt ctgcccggtc tcccgtgcgc ccggcgctgc cagattctcg agaatttttt
     6961 ttgtttcccc gagcggatcg cgcttctcga aataaacttc cattctgcct tgaggcttga
     7021 acctttcgta gagcccggat ttcggagaga acgaaaacgc ggccatttta gagcctatgt
     7081 ccgctttctc gatgccgagt ttcttcatca ttatcttgag tccgatgata tctatgaggg
     7141 aatgggcggg cgggggtatc tcgccgaacc tgtcgcggag ctcccctgtt atctctctta
     7201 aaccctcctc ggtagttata tacgagagcc tcttgtaaac gagcagcctc tccgtatcgt
     7261 tcgagatata ttcgtcggga atgaacgcgg gcgtgctcgc ggatatatcg ggctcgggct
     7321 cttccagcgg gaccttccct tcgagcctcc tcactgcccc ttcgagcatg tcgaggtaga
     7381 gctcgagccc cacgtccgct atgtgtcccg actgctcgac gccgaagagc tgacctgccc
     7441 ccctgatctc gaggtcggat aacgcaagct taaatcccga gccgagctcc ctgaattcgg
     7501 agatcgcctt gagcctcctc ctcgcgtcga gcgtgagtga ctccgtaccc ggcacgagaa
     7561 agtaggcgta cgcctttttg tccgaccttc caacccttcc tctcagctgg tagaggtcgg
     7621 caagcccgaa cgtatgggcg tcgtctatga ttatcgtgtt cgccctcggt atatcgaggc
     7681 cggattccac gatcgccgtc gtgacgagta tgtccactcc gccttcgatg aatttggaga
     7741 tagacctttc gaggtccctc tccctcatcc tcccgtgagt gacctctatc ttcgcctcgg
     7801 gaaatagttt cctcacccgg tcggcgaccc ggtaaatgtc ctcgatgcgg ttgtggatga
     7861 agaatacgca ccctccgcgg tcgagctctc tcgtaatggc ttcctcgatt acggccgggc
     7921 tagaatgatg tatatacgtc tctatggtct gccttccctc ggggggcgtg tttatgaggc
     7981 ttatgtccct tatcttcgcg agcgaaagct ggagtgttct cggtatcggg gtcgcgctga
     8041 gggagatcgt atcgacccct tcctttatct tcctcagctt ctccttctgg gagaccccga
     8101 acctgtgctc ctcgtctata atcacgagcc ccaggtcctt gaaccttatc ttctcgccga
     8161 ggagcttgtg cgtgccgatt attatgtcta ttttcccgtc ttccagcctg gagcgaatgc
     8221 cgctctcctc cttccccgtc ctgaacctgg acagtgcatc caccactacc gggtagcccc
     8281 tgagtctcga gacggcggtc ttgtagtgct gctccgcgag gagcgtcgtg ggtacgagga
     8341 acgcgacctg cttcccgtcg gtcaccgccc tgaacgccgc cctcagcgcc acctccgtct
     8401 ttccgaaccc cacatccccg cagatgagcc tgtccatcgg gcgcggcgct tccatgtcgt
     8461 tcatcacctc ttcgatagcc gcttcctggt cgggcgtttc ttcgtaggag aaaccgagct
     8521 cgaactcccg gaacatctcc ccccgcggta agtagccgta tcccttgagc gctttcctcc
     8581 tggcgtaaag ctcgagaagc tccctcgcca cgttctcgac ggcggccctt accctcctca
     8641 cgcgcttctt ccagctctcc tgcccgagcc tgtctacctt cgtggacttg ccctctccta
     8701 tgtacctctg gacgagcttg agcctgtcta ttggcacata caccttgtcc ccgcccgcgt
     8761 attcgcactg tatgaagtcc ccttccgagt cccctatcct gagcctcttc aggctcctga
     8821 atatgccgat gccgaagtcc acgtgcacga tataatcccc gggcttgagc tcgctgaagg
     8881 atgtgatgaa cgccccgggc acgtccgctc ccctcccgcg tgagacccgc tctctcttct
     8941 ccccgaatat gtccccttcc gttacgattt cgaacttccc ttcggagagc ttaaaaccgt
     9001 gcgagagctc gcccgtatgg gcgtttatct tcgtgatccc cctttccctt aagagcatga
     9061 gcagcttctc gagctccgta tgtgttctga atacgaatag gagcgtaaag ccctcctgcc
     9121 gggcttcgta aattcgctcg tcgattgcgt ccaagggaga ttcggattcc tttctcacct
     9181 ctatagaggc gggctctcct ccgaacctta tgccatccat ccttccttcc gcgtattcga
     9241 ggtcgtgaat cataatattc cggaacctgc ctgaggattc ccctacttct tcctccgtga
     9301 agtagagctc gtccatgccg ggcgctattt tgatgttctt cgttaaataa tttttcgtaa
     9361 gtccgagcga ctccctccat ctctctacgg atttcgggct gtcctccgcg tcttccgtga
     9421 tgatgagcgc gtcctcgggg aggaaatcca gcgccgagcc cggctcgggg taaaatgccg
     9481 ggagcagcca ctcgatgttc tgtaccctta tgccgctctc gatgtcctct attaagggga
     9541 gcttctccct cgccgtgacc ccgctttccc cggccctctt tttaaggtat tcgaccgccc
     9601 ggccgagcgg ctccggctcc ataatgattt cagaggcggg caaaagctcg gcgctctcga
     9661 ccctgtcggt ggatttctgg ctctcgaggt tgaaacgcct tatagagcct atctcgtccc
     9721 cgatatattc gagcctcacg ggatgcccgc tccccgggga aaagacgtcc actatcgatc
     9781 ccctcctgct tatatcccct tcgttctgta cgaagtccgt gagcgtatag ccggatttga
     9841 caagtccgga tataagctcg tccctgaacg atagcaatcc tttccctacc tttatgacgg
     9901 attttgagaa gacgtctctc ggtattgtct tgtcgaagag ggcttgagac tcggcgacga
     9961 gcacttcgcc gtttatcgcc gaattgagcc aggctatgcg gtcggagccc gattttgcgg
    10021 cggccgtgga aaatacgggc ctccccgtcc ctatctcttt tcttaacagg acacgcggcc
    10081 tttcgcccag aaagaacgag aggtcgtgag aggctgtatc cgctcttttt gccgtcgggg
    10141 agagcacgag aatcgggcgg cgcgtcgttt ctctgagcgc gtaaacgaga aagaatttgg
    10201 acgacccgcc gagacccgag agcgggatca gtttctcccc cgactcgatc tttctcagga
    10261 ggttcctgaa cttcccgctt tcattgagcg tttttattgc gttatcgagc ttgatgcgcg
    10321 gcctgtcttt cactctgaat aagtatatcc gggaggcgtc agcctttgag ctcgcccgcg
    10381 atgagtgcgc ccatttccct gcaccccacc ttctttgtcc cttcctgata tatgtcggcg
    10441 gtcctgtacc ccttttcgag cacggtcttg acggcgtttt ctatatcgtc cgaggccccg
    10501 tccctgttga acgtgtattt gagcatcatc gccgaggtca ggagcgacgc tatcgggttt
    10561 gcgatgtcct gccccgctat atcgggcgcg ctcccgtgga ccggctcgta gatcgccccc
    10621 ttgcctatgc tcgccgatgg cagcatcccg agagaccccg taagctgcgc ggcttcgtcg
    10681 cttatgatgt ctccgaacaa attgcccgcc agcatgacgt cgaagctcct gggcctcctt
    10741 atgagctgca tcgccgcgtt gtcaacgtaa agatgctcga gttctatgtc cgggtattcg
    10801 gcttcccgga ccctcgtcac aacggtcctc caaagctgca tgacctcgag tacgttcgcc
    10861 ttgtctattg acgtcacctt cttctttcgc ttcctcgcta tatcgaaagc gaccctcgct
    10921 atcctctcta tctcccgggt cgtatatacg agcgtgttgt aacccttttc cccgccgccc
    10981 gggagtttat cgatcccacg cggggttccg aagtatatac cgcccgtgag ctcccttacg
    11041 accataatgt cagccccctc cactatctcc ctcttgagcg acgaggcatc tgcgagcgcc
    11101 ggatacacaa cggcgggtct caggttggcg aatgcgtcga gctcttttct gagggcgagc
    11161 aggccggcct cgggcttctt cgagtgctcg aggttctccc atttgggtcc cccgaccgcc
    11221 cccatgagca cggcatccga ggcgcgggct ttctcgagca cttcttccgt tatagggacc
    11281 ccgtgcgcgt ctatcgagct tccgccgaac agctcttttt cgaattcgaa gtctatattg
    11341 tgctttttcc ccagtatatc gagtatgttg agactcacgt ccgtaacctc gacgcctatt
    11401 ccgtcaccgg gcagtactaa tactcttggc aatctgattc tcctttttct gtgctttttg
    11461 agacgcaccc tattttaccc acgatagttt tcaagagaaa gcccctgggg gggcgcatac
    11521 acggcgtttg aaataaaatt gcctcaagcg tagaattctt tttcatcacc gggcgcagtc
    11581 ccggctgcgg ggaggcgctc cttcccgtga ggcggattaa gtgagcttta ttcggagaaa
    11641 gtggctcgtt tctcttctcc tatttcttgt ttccctggga gtctattcac cctccctcag
    11701 gaacggcttc gtctgggacg atgtggagac gatagagcag agctactata tctacaacgc
    11761 gtccagcatg ggcgccatgt tcatcccccc cgagaaagag tccaaggacg cgctctatta
    11821 ccgtccgctt atatacgcct cgatggttct ggacaaagcc ctctggggaa taacaccttt
    11881 cggcttccac ctgagcaaca tagtcctgaa cgcgttttcc acggtgtgtg cgtactggtt
    11941 cttgctcctc ctgctcggcg tattcggttg cgagagaagg tgcggggccg cgtttatagc
    12001 gggatttctc tttgctttct atcctatgca cgtggagtcc gtatcctggg tgtccgggcg
    12061 ctccgacgtc ctctgcgcgc tatttttctt cctcgccctc attttccata tcctttcgtt
    12121 gagaaagacg ggcttccttg tgctgacggc ggccttcttc gctctgtctc tttcgtcaaa
    12181 ggaggtcgcg gtcgtcttcc ccgtaacggc gctcgcgttc gatctcatta acaggagtaa
    12241 ggggagaaaa gcgaatattc tcagatacgt ggtgtacgca tcggtgctgg ctctcttctt
    12301 ctatctgagg ggaaggacat acgtcaattt ccccgagcta tcgagcgtac gcgttccggg
    12361 cgggacgccc ggccacgggg acgttatccc ggcacccggc atactcggcg cggtgaaggt
    12421 cgttctcgtc tcgtacatgt actacttcac gaagctcgtc tttccataca cgtttaacgc
    12481 gttcacggcg aaagcgcccg gcgggcctgc ctacgtcatt tcatcggtta tgacgttcgg
    12541 attcctcctg ctcggagttt tttattcgat aaagaagcga tacaacgtga gcgcctttgc
    12601 gctcatctgg atacccgtca cgctcctccc gtcggtcctg atagcgttga ccgatatccc
    12661 gtccgcgtcc gtcgcggaaa ggtatctgta cataccctcg gctgggttct gtctcttcct
    12721 tgcgtggata ttcacgcgcg cgcttgtgtc cgtcagacgc gacagaatcc tgctggccgc
    12781 ggcttttatt ttgaccgcct cttatttatt ctttaccgtc gaccgccagc gcgtatggag
    12841 agacggcgtt tctttatggg gggacacggt cgggaaatcc cccgggagcg ccattcctca
    12901 tatcaattac ggtctcgcgc tgctcgacga gggaaaggat gacgaagccc tgaaagagct
    12961 cctcaggaat ttcgatgaag gtgtcgagat cgacgataga ggtcggtcgg ttactgccag
    13021 caatatcggt gtcgtgtata ttaacaaaaa agacgtccgt aacgccgaaa aatggttcct
    13081 caaagcctac gggtacaatc ccggatatta taaaacgagc tatcatctgg ggctcgtctt
    13141 gtacttgagg gggaaatccg gcggatcgag ggaggattac gtaagcgctc tcgggtatct
    13201 gaacaagtcg cttcagatga ggccctttta cgggaaggcg taccttcttc aggctatggt
    13261 ttacggcgag ctcggagagc gggataaggc gagagagagc gcggagcgcg ccctcgaaag
    13321 cggtttgacc gataagctcg ccgcgaaggc ccggaaaata ataagcgcgc ccgactgatt
    13381 gcctgataag cgggacccgg aaccccgtta caaaaaaaat cgccctcgta tagcaaaatg
    13441 acagctaaga aaagaaagaa atcaaaacag gatatgggag agaagccgtc ccccgtatcc
    13501 cccgcagctc ccggacatga gaacggaagg atccg
//
LOCUS       DASSJH010000387         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_202670, whole genome shotgun sequence.
ACCESSION   DASSJH010000387 DASSJH010000000
VERSION     DASSJH010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202670"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VFJ67_08580"
     CDS             complement(<1..1344)
                     /locus_tag="VFJ67_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7290477.1"
                     /translation="MNERSRLAALRKDGTQFPAEASISRQATGDGMMFTAILRDVSTQ
                     ENYRRELEHALSLLRATLDATADGIISIDTSNRITSLNRKYIEMWQVPESVAAAKDFG
                     LLIEHLLSQVKDPQSIRGTARHLEKCPEKAIHEFIELRDGRVFEHSSNPQRTGAEIVG
                     VVCSYRDVTDERHLVEELSESLTRLSKKNRYESIIGSISRSVTESHNLQEILEDIVDA
                     ISRNINPSYIVSIYFVEGGEAVLKASRGYHGWYIKEAGRIPYPKGFTWKAIMEDKPLL
                     YCSDVDQDMAIGPAGRKLGTKSYVSMPFSHGGEVTGTINIHSLKRDAFDEEELNLLET
                     VAQQIERAIKKIRMTEALRQSEERYRILFDQSPVGVYIFDRDLNVTQCNKRMTEILDT
                     SYENIIGFNIRNLKDKSFVPATEKTILGEHSYREGVYEATTSGARVWLSQHFSPLR"
     gene            complement(1387..1632)
                     /locus_tag="VFJ67_08585"
     CDS             complement(1387..1632)
                     /locus_tag="VFJ67_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7290478.1"
                     /translation="MKHRSVKKKELAEETENLRKPSSRQDSILNIAEDAIVSVDRDQK
                     IVFFNNGAENIFGYTRDEILGKPLEVLIPGRFVEEHR"
     gene            complement(1991..>2573)
                     /locus_tag="VFJ67_08590"
     CDS             complement(1991..>2573)
                     /locus_tag="VFJ67_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290479.1"
                     /translation="IKEFYPELYAQILSDTRAAILSGGNMEDIIADNGQKLAALLQND
                     LPLASPDATSAFITSFIHVFEKAGEKDPACCVDLINGDDRCMWSLMTAEEKDEVLRAI
                     ALIIRSARTNPTKLTNQKQAVKDVGRISSDVVAKYGPEIDYTAQSPLTDEEKKKVCFA
                     IADLYSGTLKLPPQRSSDAIKYLLSGTGEQPQQ"
BASE COUNT          448 a          696 c          749 g          680 t
ORIGIN      
        1 gcggagcggc gagaagtgct gggacagcca taccctggct ccgctcgttg tggcctcata
       61 cacgccttcg cggtacgagt gctcgcccag gatcgtcttc tcagtcgcgg ggacgaagct
      121 cttgtctttg aggtttctga tattgaaccc gatgatgttt tcatatgacg tatcgagtat
      181 ctctgtcatc cgcttgttgc actgggtgac gttgagatcc ctgtcgaaga tataaacgcc
      241 gacgggcgac tggtcgaaga gtatgcggta gcgctcctct gactgcctca gcgcctccgt
      301 catgcgtatt ttctttatgg ccctctcgat ctgctgagcc accgtttcca gcagattcag
      361 ctcttcctca tcgaacgcgt ccctcttgag ggaatgtatg tttatcgtcc ctgtgacctc
      421 gccgccgtgg ctgaacggca tcgatacgta gctcttcgtc cccagcttcc tgcctgcggg
      481 cccgatggcc atgtcctggt ccacgtccga gcaatacagc aagggcttgt cctccatgat
      541 cgctttccag gtaaagccct tcggatacgg tatcctgccg gcctccttta tataccagcc
      601 gtgatagcct ctcgatgcct tgaggacggc ttctccgcct tcgacgaaat agatcgaaac
      661 gatgtaggac gggtttatgt tcctgctgat cgcgtcgacg atgtcttcca gaatttcctg
      721 aaggttatgg gattcggtaa cgctcctgct gatcgagccg attatcgact cgtaccggtt
      781 cttcttcgaa agccgcgtga ggctctcgct gagctcttcg acgaggtgcc tctcgtccgt
      841 aacgtcgcgg tagctgcaga cgactccgac tatttcggcg ccggtacgct gggggttgct
      901 cgagtgctcg aataccctgc cgtccctgag ctctatgaac tcgtgtatcg ccttctcggg
      961 gcacttctcg agatgtcttg ccgtcccgcg gatgctttgg gggtccttta cttgagagag
     1021 aaggtgctct ataagcaggc cgaagtcttt cgccgccgcg accgattcag gaacctgcca
     1081 catttcgatg tacttccggt tgaggctcgt tatcctgttc gacgtgtcta tggagataat
     1141 gccgtccgcc gtggcgtcga gcgtggcgcg gaggagggag agcgcatgtt cgagctctct
     1201 tctgtagttt tcttgagtac tgacgtcgcg gagtatggcc gtgaacatca tgccgtcccc
     1261 ggtggcctgc cgggagattg aggcctcggc cgggaactgc gtgccgtcct tccgcagtgc
     1321 agcaagtcgg gaacgctcgt tcatgtactt tgaagatacg gggccgcggg cgaaattctc
     1381 tacgtgctat cggtgctcct cgacgaaacg gccgggtatg aggacttcga gcggcttgcc
     1441 caggatttcg tcccgggtgt agccgaatat attttccgcc ccgttattga agaagacgat
     1501 cttctggtcc cggtcgacgg acacgatggc gtcctcggcg atgttcagga tgctgtcctg
     1561 gcgggagctc ggctttcgca ggttttcggt ctcttccgcg agctcttttt tctttactga
     1621 tctatgtttc atttcctcgg cacgcctcgc gagtgctttg ccgtgtcgac gtacctatcg
     1681 acgccgggca tgaatcacga ctttaacttc ctgacctaag tgccggaacc gaaagcatta
     1741 tagacatcgg aggacgattc ggggtgatgt gagaaggtaa tttattcatc cggcgaatag
     1801 aactgaaccc tgaatttttt attgcttaat cctggataaa atccgttatt attgcttaat
     1861 tatagtgcct tttatcaaaa ttaggctcgt attggcccaa tgtgctgtat ttagtcggat
     1921 tgccggtaat gttggtttga tcgggcaggt tggatgaggt cggagatgag cgcacattgt
     1981 gcgctatgcg ctattgctga ggttgctcgc cagtgccgga gaggaggtat ttgatcgcgt
     2041 cggaggagcg ttgcgggggg agcttgagtg tgcccgagta aaggtcggcg atggcgaagc
     2101 agaccttctt cttctcctcg tccgtgagag gcgactgcgc ggtgtagtcg atctccgggc
     2161 cgtatttcgc gacgacgtcc gatgaaatcc tgccgacgtc cttcaccgcc tgcttctggt
     2221 tcgtgagctt tgtcgggttt gtgcgcgcag accttattat gagcgctatc gcccggagca
     2281 cctcgtcctt ctcctctgcg gtcatgaggg accacatgca acggtcgtcc ccgtttatga
     2341 gatcgacgca gcaggcgggg tctttctccc cggccttctc gaacacatgg atgaacgacg
     2401 tgatgaatgc cgaggtggcg tccggggagg ccagagggag gtcgttctga aggagcgccg
     2461 cgagcttctg cccgttgtcg gcgattatgt cctccatgtt ccctccgctg agtattgcag
     2521 ccctcgtgtc ggagagtatc tgggcgtaga gctccgggta gaactcctta atc
//
LOCUS       DASSJH010000388         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_419140, whole genome shotgun sequence.
ACCESSION   DASSJH010000388 DASSJH010000000
VERSION     DASSJH010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419140"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFJ67_08595"
     CDS             complement(<1..201)
                     /locus_tag="VFJ67_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290480.1"
                     /translation="MPTSQSQLPRCAMRCASERLVLILSSVSSASLRSVMSSATAMPP
                     MTFPCSSQSGEKCWDSHTLALLV"
     gene            357..2066
                     /locus_tag="VFJ67_08600"
     CDS             357..2066
                     /locus_tag="VFJ67_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7290481.1"
                     /translation="MYSIDHRIVLPDGSERVVHEQAEVVFDDYARPLRMIGTVLDITG
                     LRQTEKALREHERMLSTVLNSLPGCTYRCRNDEEFSFEFASEGLTALTGYTIEELRNK
                     SVNYLDIINPDDLPPLLSDVYEALEKHRMYELVYRIRTRAGEEKWVWDKGQGVYSPSG
                     ELLYLEGFVTDITKRKRLEERLAQSQKMEAVGKLAGGIAHDFNNLLTVVLNYSDLLLS
                     RPLVDKKQEEGIREIRSAGQRAAALTSQLLAFSRKQILQPKVLDLNGVVTGMERMIRR
                     LIRENIELVTELEPALGRVKADPVQIEQVIMNLAINAGDAMPRGGRLHLQTSNVSQND
                     ASAVLKPHLPPGRYVMLAVSDTGHGMDEATLSQIFDPFYTTKELGKGTGLGLATVYGI
                     IKQSGGHVTVDSGRDRGTTFKIFLPAVEGQPEQAGESAGITARPQGGPETILIAEDQQ
                     EVLSLVSLVLEEAGHKVLEANNCIEAVRTCEKFEDPINLLLIDVVMPQMSGPELARRL
                     TALHPEMKVLYMSGYIDNTIDEYGVFSSGSSFIQKPFTPALLLGKVREVLDSQPGRPV
                     PVQ"
BASE COUNT          537 a          682 c          696 g          407 t
ORIGIN      
        1 aacgagcagg gccagggtat ggctgtccca gcacttctcg ccgctctgcg acgagcaggg
       61 gaacgtcatc gggggcatgg cggtcgccga ggacatcacc gagcgcaagg aggccgagga
      121 gacgctcgag aggataagaa cgagtctctc ggacgcgcag cgcatcgcgc acctcggcaa
      181 ctgggactgg gacgtcggca cgaaccggct cctctggtcg gacgaggtct accgcatatt
      241 cggatacgcg ccccaggagt tcgaggcgac gtacggatcg ttcctcaatt ccgtccaccc
      301 ggacgacagg gatttcgtaa tgaagtccgt cgacgaagcg ctctacaagg ggggcgattt
      361 acagcatcga ccaccggata gtgctgcccg acggctcgga gcgggtcgtc cacgagcagg
      421 ccgaggtcgt cttcgacgat tacgcccgcc cgttgcgcat gatagggacc gtgctcgaca
      481 tcaccggcct caggcagacg gaaaaggccc tcagggagca cgagcgcatg ctctcgacag
      541 tgctgaacag tcttcccgga tgcacatacc gctgcaggaa cgacgaagag ttctcgttcg
      601 aattcgcgag cgaaggactt acggcgctca ccggctatac gatcgaagaa ttaaggaaca
      661 aatccgtgaa ctacctcgac attatcaacc ccgacgacct tcctcctctc ctgagcgatg
      721 tgtacgaggc gctcgaaaag cacaggatgt acgagctcgt ctacaggata aggacgaggg
      781 ccggcgaaga aaagtgggta tgggacaaag gccagggcgt ttactcgccc tcgggagagc
      841 tcctttacct ggaaggcttc gtcaccgaca tcacgaagag gaagcgtctc gaggagagac
      901 tcgcgcagtc acagaaaatg gaagccgtcg ggaagctcgc gggcggtatc gcccacgact
      961 tcaacaacct gctgacggtc gtactgaact acagcgacct ccttctctcg cgccctctcg
     1021 tcgataaaaa gcaggaggag ggcatacggg agatcaggag cgcggggcag cgcgccgccg
     1081 cgctgacgag ccagctcctg gccttcagcc gcaagcagat actccagccg aaggtgctcg
     1141 acctgaacgg cgttgttacc ggcatggaaa ggatgatacg gcggctcatc cgcgagaaca
     1201 tcgagctcgt caccgagctc gagcccgcgc tcgggcgcgt gaaagccgac ccggtgcaga
     1261 tcgagcaggt gataatgaac ctcgctatta acgccgggga cgccatgccc aggggcggca
     1321 ggctgcatct acaaacgtcg aacgtttctc aaaacgatgc ttcagcggtc ctcaagcccc
     1381 atctcccgcc cggccggtac gtgatgctcg ccgtgagcga cacggggcac ggcatggacg
     1441 aggcgacgct ctcgcagata ttcgacccct tctacacgac gaaggagctc gggaaaggga
     1501 ccgggctcgg cctcgcgacc gtttacggga taatcaaaca gagcggcggg cacgtaacgg
     1561 tcgatagcgg aagggacagg ggcacgacgt ttaaaatatt cctccccgcg gtcgaaggtc
     1621 agcccgagca ggccggggaa agcgcgggta taaccgcgcg cccgcaggga gggcccgaaa
     1681 ccatacttat tgccgaggac cagcaggaag tcctgtccct ggtcagtctc gtattggaag
     1741 aagccggcca taaggtgctt gaggcgaata actgtatcga agcggtccgg acctgcgaga
     1801 agttcgagga cccgatcaac ctgctcctca tcgatgtggt tatgccgcag atgagcggcc
     1861 ccgagctcgc ccggcggctg acggcgcttc accccgagat gaaggtcctt tacatgtcgg
     1921 gctacatcga taatacgatc gacgaatacg gcgtcttctc gagcggatct tctttcatcc
     1981 agaagccctt cactcccgcg ttactcctcg gcaaagtgcg cgaggtcctg gactctcagc
     2041 ccgggcgtcc tgtacctgtc caataacgta tcgctttttc ctgtcattgc aatgattgcg
     2101 ataacgattt cgaacaagtg aggaatcggc ctatcttggt tgatggcaga taagccagtc
     2161 atttaaagcg aaataggttc tgaaaccaaa acagaagagc aatcggtcgc cccgggctaa
     2221 ttgcaagtac gccacttaat ccttgtacta gcgccagaca atccaaatta cggagctgac
     2281 gcgagtcagc gtagcgcggc aatctcatcc tttgccttta at
//
LOCUS       DASSJH010000389         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1500838, whole genome shotgun sequence.
ACCESSION   DASSJH010000389 DASSJH010000000
VERSION     DASSJH010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500838"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VFJ67_08605"
     CDS             complement(<1..1411)
                     /locus_tag="VFJ67_08605"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HET7290482.1"
                     /translation="MSVFGDDEPDPSAVAQGPVEGESTLNVVLGLERSQDLLADRIEE
                     IHDPGSPYYHDFRSVSEIAEEFGADDSVIDSVQEYLHQQGISLEADITGGFIFGQATV
                     GQLNRLFSTELYFYKINKDTFIAPLTPPTLPSELRGLVTEVLGLSTEPSLWEVPGPDS
                     SEAVAVDTRAEIETPGSPTVTGTREGCEDALKRKGFTPNQFRTAYGIDDLHQQGYHGE
                     GITMALVEGSRFDQSSIDTFTSCFGISDPVLPNVVQLGSGTLKRSGEPFLDIETIMIV
                     APALSGLYVFQTDLQSLADWVVLFASPLNPGNTGGVPVQILSASLGNCEIKWGYTPIH
                     MLEYVFMSAAASGVHTFISAGDSGSSTCYHHDKTTKTRSVEYPASSQFVTGVGGTNLA
                     LHEDNTIKGEGVWNDKDFPPPYNKKPGAGDGGKSKFLKRPSWQIGTKIPSAMRTTPDV
                     AFFADPNPGYVINDSKKGWF"
     gene            complement(1605..>2226)
                     /locus_tag="VFJ67_08610"
     CDS             complement(1605..>2226)
                     /locus_tag="VFJ67_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7290483.1"
                     /translation="DNPGLLVQTFDSAISSGCDVPFDSTNSADCFPPGALLPGVVYDV
                     APSHEFLDIFGINFNGSSGNSSKAIGPDNHTDTLDLIFDGGVSAAGVRPGCFILTGGI
                     CQRTITISVYGTGDVLLGSTQVDASSLFDDFVGIESNVLIAKISVSGPDLGPDSGFHA
                     ISELDFPLSQLQNVPTLSEWGMISAAAGIVLVGMFFAARRKRNNPL"
BASE COUNT          510 a          583 c          616 g          517 t
ORIGIN      
        1 caaaccagcc cttctttgaa tcgttaatga cgtatccggg attggggtcg gcgaaaaatg
       61 cgacgtcggg cgtggttctc atcgcactag ggattttcgt gccgatctgc caggaaggcc
      121 ttttgaggaa cttgcttttc ccgccgtctc cggctcccgg ctttttgtta tatgggggcg
      181 ggaaatcctt gtcgttccag actccctcgc ctttgatcgt gttgtcctca tggagcgcca
      241 gattggtccc tcccactcct gtaacgaatt gcgacgaggc cgggtattcg actgatcttg
      301 tcttggtcgt tttgtcgtgg tgatagcagg tcgaggagcc gctgtcgccg gccgagatga
      361 acgtgtggac tccagaagcg gccgcgctca tgaacacata ttcaagcatg tgtatgggcg
      421 tatatcccca tttgatctcg cagttgccga ggctcgcgga cagtatctga accggaaccc
      481 ctccggtatt cccggggttc agcggcgagg cgaaaaggac gacccagtcg gcaagcgatt
      541 ggaggtctgt ctgaaagacg tataacccgg acaaagcggg ggctactatc atgatcgttt
      601 caatatcgag aaatggctcg cccgaacgct taagtgtgcc gctccccagc tgaacgacgt
      661 tcggcagcac ggggtccgaa attccgaaac aggatgtgaa tgtgtcgatg ctcgattggt
      721 cgaatctcga tccttcgacg agggccatcg ttatcccctc cccgtgatag ccctgttgat
      781 gaagatcgtc tatgccgtag gctgttctaa attggttggg agtgaagcct ttccgcttta
      841 acgcatcttc gcacccttca cgggtgccgg taacggtcgg ggatcccggc gtctcgattt
      901 cagctcttgt gtcgacggcc actgcttcgg aagaatcggg cccggggact tcccagagag
      961 acggttccgt acttaggccc agcacctcgg tgacaagccc tctgagctcg gacggaagcg
     1021 tgggtggagt caaaggcgcg atgaaagtgt ccttgtttat tttatagaaa taaagctcgg
     1081 tgctgaagag tcggttcagc tgcccgaccg tcgcctggcc gaagatgaat cctcctgtta
     1141 tatcggcctc gagtgagata ccttgctgat gcagatattc ctgcacgctg tctatcaccg
     1201 agtcgtccgc tccgaactct tctgcgattt cggatacgct cctgaaatcg tggtagtagg
     1261 gggagcccgg atcgtgaatc tcctcgatcc tatcggccag taaatcctgg gagcgctcga
     1321 gcccgaggac tacgttgagc gtcgattcgc cctcgaccgg gccttgggcg actgccgagg
     1381 gatcgggttc atcgtctccg aaaacgctca tagtgtccga attgtcggag tcgcaggaaa
     1441 agcaaaacag aaaaacaaaa atcgccggaa gaatatattt tttcattacc gaattataat
     1501 gacgttttta ccggtttgga cattcatgat tgaggagttc aaataaccga tagaaggtga
     1561 gaaatgaatt taaaaaggca caatgatccg aaaagagcct ggagtcaaag gggattattc
     1621 ctcttacgcc ttgcagcgaa aaacatgccg accaatacaa ttccggcggc ggccgagatc
     1681 atcccccatt ccgacagcgt gggcacattc tgcagctggc tcagtggaaa atccagttcc
     1741 gatatagcgt ggaagccgga gtcaggcccg agatcgggcc cactgacgct gatttttgct
     1801 ataagaacat tcgactcgat tccgacaaaa tcgtcgaaaa ggctcgaggc atccacctgc
     1861 gtagagccta aaagcacatc tccggttcca tagacgctaa ttgtaatcgt tctctgacat
     1921 attccacccg ttagtatgaa gcatcctggt ctcacgcccg cggcggacac cccgccgtca
     1981 aatattaaat ccagagtatc ggtatggtta tcgggcccga tcgctttcga agagttacca
     2041 ctcgagccgt tgaagttaat gccgaagatg tcgaggaact catgtgacgg agccacatcg
     2101 tacacaactc cagggagcag cgcgcccgga gggaagcagt ccgcactgtt ggtcgaatcg
     2161 aagggtacat cacaaccgga ggagattgcg gaatcgaatg tctggaccag cagtccggga
     2221 ttatcg
//
LOCUS       DASSJH010000390         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_627632, whole genome shotgun sequence.
ACCESSION   DASSJH010000390 DASSJH010000000
VERSION     DASSJH010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627632"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFJ67_08615"
     CDS             <1..604
                     /locus_tag="VFJ67_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HET7290484.1"
                     /translation="RLTVKGVLNNVSFNTPGCLIFDIGGGSTEYVYVNDGKIMNITST
                     GLGVVRLTEKYLADENDTEADMAALDAHIESVITEALPSFPQGGDETLTLIGTAGTPT
                     TLAAMELRLAAYDPVLVNNYVLTREKIERTLKTVLSLPRADRTKIPGLEKGREDLIVS
                     GTAVVLKTMERFMSGRMAVSDAGLLEGIAYSMVGGGKAGR"
     gene            786..1418
                     /gene="grpE"
                     /locus_tag="VFJ67_08620"
     CDS             786..1418
                     /gene="grpE"
                     /locus_tag="VFJ67_08620"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET7290485.1"
                     /translation="MNPDDDDKEQGDMELSDNGDNYNEEESESTTEERASGDKDYQDL
                     YQKYIRLAADFENYKKRLAKEKADVVAYGNEELIKALLNVIDNLERALDHADEEADPK
                     ALIEGVKLVHKQFLSCLEKFGVKFVEATPGQEFDPRMHQAIERVESPDFTPGLIISEM
                     LRGYMLKERLLRPALVVVSKGPSGPGEPEQSRGGNGSGGPEDVFDLTGGE"
     gene            1443..>2546
                     /locus_tag="VFJ67_08625"
     CDS             1443..>2546
                     /locus_tag="VFJ67_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7290486.1"
                     /translation="MAIVGIDLGTTNSCVAIIEGGSPVVIPNEEGSRVTPSVVAFTED
                     NSRFAGAVAKRQAVVNPTTTIFGVKRLIGRKFESPEVQKAKHLAPYDIIPNESGDAWI
                     ETNSRTYSPQEISSIVLSKMKQVAEEYLGYEVTDAVITVPAHFNDLQRQATKDAGRIA
                     GLNVRRIINEPTAAALAYGFDKIENKRIAVFDLGGGTFDITILEIANGVFEVKSTSGD
                     TFLGGDDFDQMLIELVLEDFKNHHEIDLREDKMALQRIRETCEKAKQELSSIHETAIN
                     LPFIAVDQTGPKHLNYKINREEFERLVGELVDRLEIPCKQALEDARLDPSGIDEVVLV
                     GGMTRMPRIQEKVRQIFKKEPQTRINPEEVVAIG"
BASE COUNT          690 a          640 c          768 g          448 t
ORIGIN      
        1 gaggcttaca gtcaagggag ttcttaacaa cgtcagtttc aatacgcccg gctgcctcat
       61 attcgatata ggcggcggga gcaccgaata cgtgtacgta aatgatggaa agataatgaa
      121 catcacgagc acgggactcg gcgtcgtgcg tctcacggaa aaatacctgg ccgatgaaaa
      181 cgataccgag gcggacatgg cagctttgga cgcccacatc gaaagtgtca taacggaagc
      241 ccttccttcg ttccctcagg gaggggatga gacgctcact ctcataggca cggctggcac
      301 tccgaccacg ctcgcggcta tggagcttcg tctggcggct tacgatcccg ttctcgttaa
      361 caattatgtc ctgacacggg aaaagataga gagaactctg aaaacggtat tgagccttcc
      421 gagagccgac aggacaaaga tacccggcct ggaaaagggg agggaagacc taatcgtttc
      481 cggaactgct gtggtgctga aaacaatgga gcgctttatg tcgggcagga tggccgtgag
      541 cgacgccggg ctcctcgaag gcatagccta cagcatggta ggcggcggga aagccgggcg
      601 atgatttccg ggccgtcgtc gttgacaaat tatagaggga ttattatatt ttgcataagg
      661 gtttaggatc actgccatct gctgaaaatt atctataaaa cggttacgcg gcctgctcgt
      721 aatagcgcta taattatgca tgaataatga ctggttacag gtcggttgaa atcagagggg
      781 ctggtatgaa tccggacgac gacgacaaag agcaaggaga catggaattg agcgataacg
      841 gggacaacta taacgaggaa gagtccgaga gcacgactga agaacgcgct agcggggata
      901 aggactacca ggacctctat cagaagtaca ttaggctcgc ggccgatttc gagaattaca
      961 agaagagact cgccaaggaa aaggccgacg tcgtggccta cggcaacgaa gagctgataa
     1021 aagcgcttct caacgtaata gacaacctcg agcgtgctct cgatcacgcg gatgaggaag
     1081 cggacccgaa agccctgatc gaaggggtga agctggtcca taagcaattc ctcagctgcc
     1141 tcgagaagtt cggcgtgaag ttcgtcgaag cgacgccggg gcaggaattc gatccgagga
     1201 tgcatcaggc tattgaacgg gtggaatccc cggatttcac gcccggtctc ataatatccg
     1261 agatgctccg cggatacatg ctgaaggagc ggctgttgag acccgccctc gtggtcgtgt
     1321 ccaaggggcc gagcggcccg ggcgagcccg agcagagcag gggcggtaac gggtcaggcg
     1381 ggcctgaaga cgtattcgac cttacaggcg gtgaataaaa tcaatttaag cggaaggcga
     1441 acatggcaat agtaggaatc gacctgggca ctacaaactc gtgcgttgcg atcatagagg
     1501 gcggcagccc cgttgtaata ccgaacgaag aggggagcag ggtaacccct tccgtagtcg
     1561 ctttcacgga agataactcc cggttcgcag gggcagtggc caagcggcag gccgtcgtca
     1621 acccgacgac cacgatattc ggggtgaagc gcctgatcgg gaggaagttc gaatcgcccg
     1681 aagtacagaa ggccaaacat ctcgctccat acgatatcat cccaaacgaa tcgggagacg
     1741 cctggatcga gacgaattcc cgcacgtaca gtccgcagga aatatcctcc atagtcctct
     1801 ccaagatgaa acaggtagca gaggaatacc tcggttacga ggtaacggac gccgtcatca
     1861 cggtccccgc ccacttcaac gacctccaga ggcaggcgac gaaagacgcg ggacgcattg
     1921 cgggcctcaa cgtgaggagg atcataaacg agcccacggc ggccgcgctc gcctacgggt
     1981 tcgacaagat agagaacaag agaatcgcgg ttttcgacct cggcggaggc actttcgata
     2041 taacgatact cgagatcgca aacggagtat tcgaggtgaa gtccacgagc ggcgatacgt
     2101 tcctgggagg ggacgatttc gaccagatgc taatagagct cgtgctcgag gactttaaga
     2161 accatcacga gatagacctc agggaagaca agatggcgct ccagaggata agggagacgt
     2221 gcgagaaggc gaagcaggag ctctcctcga tccacgagac cgctataaac ctcccgttca
     2281 tcgcggtcga ccagacgggc cccaagcacc tcaactacaa gatcaacagg gaagagttcg
     2341 aacgcctcgt cggggagctc gtggacaggc tggagatacc ctgcaagcag gcgctcgaag
     2401 acgcgaggct cgatccttcc gggatagacg aggtggtgct cgtcggcgga atgacgagga
     2461 tgccgcgcat acaggaaaag gtaaggcaga ttttcaagaa ggagccacag acacgaatca
     2521 acccggaaga agtcgttgcc ataggc
//
LOCUS       DASSJH010000391         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_527730, whole genome shotgun sequence.
ACCESSION   DASSJH010000391 DASSJH010000000
VERSION     DASSJH010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527730"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VFJ67_08630"
     CDS             complement(<1..1389)
                     /locus_tag="VFJ67_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET7290487.1"
                     /translation="MSEKTTTAHDSTIGDMNTDGASKCPFTGGMPKHSAGGGTSNRDW
                     WPNLLRLNILRQHSSLSNPMGDTFNYAEEFKTLDLNAVKKDLFELMTASQDWWPADYG
                     HYGPLFIRMAWHSAGTYRIADGRGGGGSGSQRFAPLNSWPDNANLDKARLLLWPVKQK
                     YGRKISWADLMILAGNCALESMGFKTFGFAGGREDTWEPEEDIYWGSEAEWLGDKRYS
                     GDRELENPLGAVQMGLIYVNPEGPNGNPDPIAAARDIRETFGRMAMNDKETVALIAGG
                     HTFGKTHGAADPSKYVGREPAAAGIEEQGLGWKNTFGSGSGDDTITSGLEGAWTTTPT
                     KWSNNYFENLFGFEWELTKSPAGAHQWKPKNGTGAGTVPDAHDPSKSHAPFMLTTDLS
                     LRMDPVYEPISRRFYENPDEFADAFARAWYKLTHRDMGPRARLLGPEVPAEELIWQDP
                     IPAVTHELIDEND"
     gene            1580..2491
                     /locus_tag="VFJ67_08635"
     CDS             1580..2491
                     /locus_tag="VFJ67_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7290488.1"
                     /translation="MELHQLRYFVAVAETGGFSKAARVCFVAQPSLSQQIKKLEQELG
                     QMLFERLGRTTVLTEAGRALLPRARVILKEAGDIKAGIKEDVSSGAGHLSVGLIPTIA
                     PYILPGALKRFYESFPRAQIGVEENLTERLVKKIIGLEIEMAVMSLPIDDRLIITESL
                     FEDPLVLALSPGHELAKSDDVSIEDLKSVPFIALDEEHCLGEQINNFCYERQINPEIV
                     CRTWNLSTIQHCVSFGSGVSLVPKMLVMTDVSNKCVYRSIEGQSPKRTVVAAWHRDRK
                     ISKLAGEFIRIVKDEYEYLVSNGARDV"
     gene            2563..>2884
                     /locus_tag="VFJ67_08640"
     CDS             2563..>2884
                     /locus_tag="VFJ67_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7290489.1"
                     /translation="MKNIYLDYNATTPLDPRVLDGMMPYFRDDFGNPSSIHSFGMKSR
                     GALDRAREQVAGLIGASPREIFFTSGGSESNNYAIKGAAYGLKEKGSHLVTTAVEHES
                     VLETF"
BASE COUNT          624 a          768 c          836 g          656 t
ORIGIN      
        1 gtcattctcg tcgatcagtt catgcgtaac agccggtatg gggtcttgcc atatcagctc
       61 ttccgccggc acctccgggc cgagaaggcg cgcgcgcgga cccatatcgc ggtgcgtaag
      121 cttgtaccat gcccgtgcga aagcgtctgc gaactcatcc ggattctcgt aaaagcgtct
      181 tgaaatcggc tcatagacag ggtccatccg caaggagagg tccgtcgtaa gcatgaatgg
      241 cgcgtgactc tttgacgggt cgtgcgcgtc cggcactgta ccggcgcctg ttccgttctt
      301 cggtttccac tggtgagcgc cggcagggct cttggttagc tcccattcga aaccgaacag
      361 gttctcaaaa tagttgttgc tccacttagt tggggttgtg gtccatgcgc cctcgagtcc
      421 gctggtaata gtgtcgtctc cactgccgct gccgaaagtg tttttccagc ccaatccctg
      481 ctcctcgatg ccagcagcag cgggttcacg accaacatac ttgcttggat cggcggctcc
      541 atgtgttttg ccgaaagtgt gtccgccggc aataagcgcg acagtctctt tgtcgttcat
      601 agccatgcgg ccgaaggtct cgcgaatgtc acgggctgcc gcgataggat cgggatttcc
      661 attcggaccc tcggggttca catagatgag gcccatctga acggcgccca agggattctc
      721 aagctcccgg tcgcctgaat aacgcttgtc tccaagccac tcggcttcgg aaccccagta
      781 gatatcttcc tcgggctccc aagtatcctc gcgtcccccg gcgaaaccga atgtcttgaa
      841 gcccattgat tctagagcgc agttgccggc gaggatcatc aggtcggccc aggaaatttt
      901 cctgccgtat ttctgtttga ccggccagag cagtaggcgc gccttgtcga ggttcgcatt
      961 gtcgggccag ctgttgagcg gcgcaaagcg ctggctgccg gagcccccgc cgccgcggcc
     1021 gtcagcgatg cggtaagtac cggcgctgtg ccacgccatc cgaatgaaga gcggcccata
     1081 gtgaccgtag tcggccggcc accagtcctg ggacgcggtc ataagctcga aaaggtcttt
     1141 cttcaccgca ttcagatcga gggtcttgaa ctcctcggcg tagttgaacg tgtcgcccat
     1201 cggattggat aaggaagagt gttggcggag gatgttcaac ctcaaaagat ttggccacca
     1261 gtcccggttg gaagtgccgc cgccagcgct gtgctttggc attccacccg taaacgggca
     1321 cttgcttgca ccatcagtat tcatatctcc tatggtgctg tcgtgagctg tagttgtttt
     1381 ttcactcata atctatgacc ccctgtggaa tattacctct tgggagtatt atccatttgg
     1441 gactatagac gtcaaatatt aattgtctat aatatctata ggaaataact ataataaaat
     1501 tccaattgat aaaagtgccg gtcatgcatg gttcgtccac tttgcattga aacggactta
     1561 cgcaggaggg cgcgaccgaa tggagcttca tcagcttaga tactttgtcg ctgtcgccga
     1621 gactggcgga ttcagcaagg cggcgagggt ctgcttcgtc gcccagccgt ctctaagcca
     1681 gcagataaag aagctcgagc aggagcttgg tcagatgctc ttcgaaaggc tcggcaggac
     1741 caccgtgctc acggaagccg gccgggcgct cctgccgagg gcacgggtca tactcaaaga
     1801 ggcgggagac attaaggccg gcatcaagga ggacgtgagc tcgggcgccg gtcatctctc
     1861 ggtcggatta atacccacaa ttgcgcctta cattctgccg ggggctctca agaggtttta
     1921 cgaatcgttt ccgagggctc agatcggcgt cgaggagaac ctcaccgaaa ggctggttaa
     1981 aaagattatc gggctcgaga ttgagatggc ggtgatgagc ctccctatcg atgacaggct
     2041 gataataacg gagtctcttt tcgaagaccc tctcgttctc gcgttatcgc cgggtcacga
     2101 gcttgcgaag tcggacgatg taagcattga agacctaaag agcgtaccgt tcatcgccct
     2161 tgatgaggag cactgtcttg gggagcagat caataatttc tgctacgaaa ggcagatcaa
     2221 tcccgagatc gtctgccgca cgtggaacct gtccacgatc cagcactgcg tttctttcgg
     2281 aagcggggtg tcgctagtgc cgaagatgct cgttatgacg gacgtttcca ataaatgtgt
     2341 ttacaggtcg atagaggggc agtccccgaa gcgtacagtg gttgcggcat ggcaccggga
     2401 caggaagata tcgaagctgg ccggggagtt tatccggata gtgaaggacg aatacgaata
     2461 tctggtcagt aacggagccc gggatgttta gggtttacag ggcttagcgc ccccgaattt
     2521 cgccctaccc gggtctgttg taaaattaat aatcaggaca agatgaaaaa tatatacctc
     2581 gattacaacg caacgacgcc tcttgacccg cgagtgctcg acgggatgat gccttatttc
     2641 cgggacgact tcgggaaccc gtcgagcata cattcgttcg ggatgaagtc caggggcgcg
     2701 ctcgaccgcg cgagggagca ggtcgctggg cttataggcg cgagcccgag ggagatattc
     2761 ttcacttcgg gggggagcga gagcaacaac tacgcgataa agggagcggc ttacggctta
     2821 aaggaaaagg ggagccatct cgttacgacc gctgtcgagc atgagtcggt gctcgagaca
     2881 ttca
//
LOCUS       DASSJH010000392         6550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_852724, whole genome shotgun sequence.
ACCESSION   DASSJH010000392 DASSJH010000000
VERSION     DASSJH010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852724"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..479
                     /locus_tag="VFJ67_08645"
     CDS             195..479
                     /locus_tag="VFJ67_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290490.1"
                     /translation="MAEIKVREMSSEGDSFGFDVEIVDSGISTRHRVQVGRADYESLT
                     GGLITPRELVEKSFEFLLEREPKESILSSFDLSVIAKYFRDYPSVIKDYF"
     gene            521..1870
                     /locus_tag="VFJ67_08650"
     CDS             521..1870
                     /locus_tag="VFJ67_08650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7290491.1"
                     /translation="MGRFFTLLILIIIIGGAIYIYPDIEWHHPVIDIKLASKDVGVKP
                     FDIVVSDKGKGLKEVSVVLVNESGETVLVNKNYPEGVSGDTLNINIDAKKLGIKNGPA
                     EIRATAIDHSRLRFFSGNKTVASRNINLDLVPPAVELLSTENYINHGGSALVIYKTSP
                     DVVRSGVTIGGYFFPGFKGNFAEDDVYLGFFAYPYNVPAGEGITLVAEDGAGNAKSVN
                     VPYTLKDVAYKKSNLDISTDFIEKVMIPISGQTGETDYKKIFLMVNSDLRKKNDAKIK
                     EVTSKSKDEILWKGAFHQLSNSQVQANFADERTYIFNGEAIDKQYHLGYDLAVTKRYP
                     IEAANDGIIVYAGDLGIYGNAVIIDHGMGVASLYGHMSSIDVKAGDEIKKNQIIGKTG
                     QTGLAAGDHLHYGVYVDGVAVRPVEWWDDKWIKDNVILKIDQAKAEFGTKSSGNTKN"
     gene            1888..2634
                     /gene="rlmB"
                     /locus_tag="VFJ67_08655"
     CDS             1888..2634
                     /gene="rlmB"
                     /locus_tag="VFJ67_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET7290492.1"
                     /translation="MIIYGRNPVKELLSQPDSGIEEILMLKDGRNEGSSEIFDLAKER
                     GVRVVFLPKDAISRISGTTSHQGVAARIADFEYSSLENILSLAESSGDRLLIVILDHI
                     EDPQNLGAIIRTVDVLGAHGVVIPKDRAASVTPAVVKASAGAVNHVLIAKVVNLSGAI
                     GVLKKKGVWIVGADPQAPVPVYGEDLGKVDIGLIIGSEGRGLGQKVKEQCDFLVTIPQ
                     AGKVASLNASVSAGILIYEIMKQRGDLKSQ"
     gene            3031..3594
                     /locus_tag="VFJ67_08660"
     CDS             3031..3594
                     /locus_tag="VFJ67_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET7290493.1"
                     /translation="MEKEPKDEYREEDVNEESVNHLIEHRIPWLLVGLLGGLLATLIV
                     SKYEGILASDVRLAFFIPIIVYLSDAVGTQTETIYVRALSKKKLSFWKYIFKESLVGM
                     GLGLISGTVLGVFANYWLKSYAIGLTIGLTMLINLTLAPVLAVVIPNLIYKRHLDPAL
                     GSGPVATIIQDLISLLVYFLTATIFLL"
     gene            complement(3607..5619)
                     /gene="ligA"
                     /locus_tag="VFJ67_08665"
     CDS             complement(3607..5619)
                     /gene="ligA"
                     /locus_tag="VFJ67_08665"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET7290494.1"
                     /translation="MKLTKKDAEKKIEKLSRELEYHNYLYYVKNAPEISDYEFDSMLN
                     ELKELEAEYPDLVKPDSPTQRVGGWVAEGFASVSHIVPMMSIDNITGIDGAYEFDKRV
                     KRLLGTDGDIEYVAEPKFDGVSASLTYDGGLLTKGATRGNGLVGEDVTNNLKTIKTIP
                     LKLKGKKGIPDLIEIRGEVLYPIESFRKLNKELGEAGEPLFANPRNAASGAIRQLDSA
                     ITASRPLTFYGWGVGEVRGIELKTEWDLVERLRDWGFKVEDHMMTCANIDEAISYQKE
                     MESARDNLPYEADGIVVKVNRRNYQKELGTTAKHPRWNIAYKFKPRQGTTKIKDIIVQ
                     VGRVGLLTPVADLEPVSIGGITIKRASLHTDDIIRAKDIRIGDTVLVERAGDVIPEVV
                     MPITEKRTGKEKEFRMPDKCPVCGTGVEKEGAYYYCPNLSCPAQLKGRILYLASRRAF
                     DIEGLGEKIVEQLLEKKLIEDPADVFYLKKEDLEPLERFAEKSASNLEAQIEKSKKVP
                     FDRFINALSIPHVGERVAQIMAENFHDIAALMNTTAPALMDIHTIGSEIADSIVHFFT
                     LKQNRRLIEKMLEAGVKIQYRKKTALSDKLKGQVFVFTGSLESMTRDEAERFVAEHGG
                     RATSSVTKKTSYVVVGSDPGSKYDKAVSLGIEILSEDEFRKMMGEL"
     gene            complement(5708..>6550)
                     /locus_tag="VFJ67_08670"
     CDS             complement(5708..>6550)
                     /locus_tag="VFJ67_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7290495.1"
                     /translation="ETEKEMYKTLGLPYIPPELREDRGEIETAIEGKLPDLIELGDIK
                     GDLHTHTKWSDGKATIEEMAERAREIGYEYIAISDHSPSQTIANGLSVERLRKKQKEL
                     DAVAKKFRDIKVIMGTEVDIKSDGTLDYPDSVLKELDVVIASVHSGFKMDRKTMTDRI
                     IRAVKNPYVHAIGHPTGRLIGERDPYDVDLDLVIEAALEYGKALEVNGSYWRLDLNDI
                     NTRKAIDAGVKVIISTDAHSTEQLLFMRYGVGTARRGWVRKKDVLNALPYEELKEKLN
                     AMRK"
BASE COUNT         1418 a         1796 c         1694 g         1642 t
ORIGIN      
        1 gcgcgagctc ctggggtcag agtcggtgtc cgtctgagcc ctcacgatca gctcagcgac
       61 ctttgttaca tcgtgcgtcc ccggatcgaa tttgcgtatc tccatgctat ttagcctgtt
      121 tcgatatttt ctgtttttgc agggttaact ctaggttata atatttccac cccggtttca
      181 aagccgggga ggttatggcg gagataaaag tcagggaaat gagctcagag ggagactcgt
      241 tcggtttcga tgtggaaatc gttgatagcg gaatcagcac aaggcacaga gtgcaggtcg
      301 gcagggctga ttacgagagc ctcacaggcg ggctcatcac tccgcgcgag ctcgtcgaaa
      361 aatcgttcga gttcctgctc gaaagggagc cgaaggagtc tattctgagc agtttcgacc
      421 tgagcgttat tgcgaagtat ttcagggatt atccgagtgt cataaaggac tatttctgac
      481 aggaccggca tagaacgaaa aaggagattc ggacagagaa atgggcaggt tctttactct
      541 acttattctg ataatcatca tcggcggcgc aatctatata tacccggata tagagtggca
      601 tcaccccgta atagatatta agctcgcttc taaagacgta ggcgtgaagc cctttgacat
      661 tgtcgtgagc gacaagggga agggcctgaa agaagtctct gtcgtcctcg tcaacgaaag
      721 cggcgagacg gttcttgtga acaagaatta ccctgaaggg gtaagcgggg atacgctcaa
      781 tatcaacata gatgcgaaga agctcggcat caagaacggc cccgccgaga tccgcgcgac
      841 cgcgattgac cactcacgtc tccgtttctt ttccggcaac aaaacggttg cgagcaggaa
      901 catcaacctc gacctagtcc cgcccgcggt cgagctgctc agcaccgaga actacataaa
      961 ccacggaggc tcggcgctgg tcatttacaa gacctcgccc gacgttgtca ggagcggcgt
     1021 cacgattggg ggctatttct tccccggctt caaggggaat ttcgccgagg acgacgtata
     1081 cctcgggttc ttcgcctatc cctacaacgt cccagcggga gagggcatta cgctcgtcgc
     1141 cgaggacggg gccgggaacg cgaagagcgt gaacgtgccc tacacgctca aggacgtcgc
     1201 gtacaagaag agcaacctcg acatcagcac cgattttatt gagaaagtca tgatccctat
     1261 ttccgggcag acgggggaga ctgactacaa gaagatattt cttatggtga acagcgacct
     1321 caggaaaaag aacgacgcga agataaaaga ggtgacctcg aaatcgaagg acgaaatact
     1381 atggaaggga gcctttcacc agctctccaa ctcgcaggtg caggcgaatt tcgccgacga
     1441 aaggacgtat atattcaacg gcgaggccat agacaagcag taccatctcg gttacgacct
     1501 ggccgtaacg aagaggtacc cgatagaagc agcaaacgac ggtataatcg tttacgcggg
     1561 cgacctgggc atctacggga acgccgtgat aatagaccac gggatggggg tcgcgtcact
     1621 ctacgggcac atgagctcga ttgacgtaaa ggcgggcgac gagatcaaga agaaccagat
     1681 cattggcaag acaggccaga ccggacttgc cgcgggcgac cacctccatt acggcgtcta
     1741 cgttgacggc gtagccgttc gtcccgtcga gtggtgggac gataagtgga ttaaggacaa
     1801 cgtgattctg aagatagacc aggccaaagc cgagttcggg acaaagagct ccggcaatac
     1861 caagaattaa gccggagagg attcggattg attatctacg gaaggaaccc cgtcaaggaa
     1921 ttactctccc aacccgattc cgggatcgaa gaaatcctca tgctgaaaga cggcaggaat
     1981 gaaggctcgt ccgagatttt tgatctcgcg aaggagaggg gggtaagggt ggttttcctg
     2041 ccgaaggatg cgatatcgag gatatcggga acgacgtctc atcagggcgt tgccgcccgt
     2101 atagcggact tcgaatactc gagcctcgaa aacatacttt cgctcgctga gagcagcggg
     2161 gataggctcc tcatcgtcat actcgatcac atcgaggacc ctcagaacct tggcgcgatt
     2221 ataaggaccg tggacgtttt aggcgcgcac ggggtcgtca tcccgaagga cagggccgcc
     2281 tctgtcactc cagccgtggt caaggcatct gcgggcgcag tcaaccacgt gctcatagcg
     2341 aaggtcgtaa acctgtccgg cgcaataggg gtgcttaaaa agaagggcgt atggatagtg
     2401 ggggctgacc ctcaggcccc cgtgcctgtt tacggggaag acctcgggaa agtggatatt
     2461 ggcctcataa ttggtagcga gggtaggggg ttaggacaga aagttaaaga acaatgtgac
     2521 ttccttgtaa cgatccctca agcgggaaaa gtggcctccc tcaacgcctc tgtctctgcc
     2581 ggaatactca tttacgagat tatgaagcag agaggcgatc tcaaatcgca ataaacggcg
     2641 gaatgcggct ttaatttgtc attgcgaggc tccaacggag ccgcggcaat ctctttcgaa
     2701 tctcctcatt tcgaccactg cttcgagcaa gggagaagta gaacgacctc agctaattac
     2761 gaattagcca catccacacc gcatctcagc ggcagaaaac aaagcaggga gaagtctatc
     2821 tttttccttt catttccctc cttttgtaaa gaacggttct gaactagtgc aagaaccgga
     2881 cgccctgccc taaaatcacc gaaggaaatt aactcccagc tcaacgttgc ctaagccaaa
     2941 ataggccagg gtggattttc ttttgatttt agttccttct tttttccagc gggcatagta
     3001 cggtggaata tgaaatagta tattcataag atggagaagg aaccgaaaga cgaatacagg
     3061 gaagaggatg taaacgaaga atccgtaaac cacctgatcg agcaccgtat cccgtggctc
     3121 cttgtcgggc ttctgggggg attgctcgcc actctgattg tatccaaata cgagggaatc
     3181 ctcgcttccg atgtgaggct cgcgtttttc atccctatta tcgtgtacct gagcgacgcc
     3241 gtcggcactc agacagagac catctacgtg cgggcgcttt caaagaagaa gctctcgttc
     3301 tggaaatata tattcaaaga aagtctggtc gggatgggtc tcggattgat ctccggaacg
     3361 gttttagggg ttttcgcgaa ttactggctt aaatcctacg ccataggtct cacgataggg
     3421 ctcacgatgc ttatcaatct tactctcgcc cctgttctgg ctgtcgttat tccgaaccta
     3481 atatataaga gacatctgga tccagcgcta ggctcgggcc ctgtagcgac gataattcag
     3541 gacctgatca gcctgctcgt gtactttctt acagctacca ttttcctact ttaatcgaga
     3601 tgatgttcac agctccccca tcattttcct gaactcgtcc tcgctcaaaa tctctatccc
     3661 gagtgatacg gccttatcgt atttagaccc cgggtcgctc ccgacgacga cgtagctcgt
     3721 cttcttcgtg accgacgacg tcgcccggcc cccgtgctcg gcgacgaatc tctcggcctc
     3781 gtcccgggtc atggattcaa gcgagcccgt gaatacgaac acctgcccct tgagcttgtc
     3841 gctcaaggcc gtcttcttcc tgtactgaat cttcacgcct gcttcgagca tcttctctat
     3901 gagccgcctg ttctgcttga gcgtgaagaa atgcactatg ctgtcggcga tctcgctgcc
     3961 tatcgtgtgt atatccatca gggccggggc ggtcgtattc ataagtgcgg ctatgtcgtg
     4021 gaagttctcg gccatgatct gagctaccct ttcgcctacg tgaggtatgc tgagcgcgtt
     4081 tatgaacctg tcgaacggga cctttttgct cttttctatt tgtgcttcca ggttcgaggc
     4141 cgacttctcg gcgaaacgct ctaaaggttc caggtcttcc ttctttagat aaaatacgtc
     4201 cgccgggtcc tcgatcaatt ttttttccaa gagctgctcg actatctttt cgccgagacc
     4261 ctctatatcg aaagccctcc tcgaggcgag gtataatatc ctccccttga gctgtgccgg
     4321 acacgacagg ttggggcagt agtagtatgc tccttccttc tcgacgcccg taccgcaaac
     4381 ggggcatttg tcgggcattc tgaattcctt ttctttaccc gtccgcttct ctgttatggg
     4441 catcacgact tccgggatga cgtcgcccgc cctttcgact aagaccgtat cgccgatcct
     4501 tatgtccttc gcccggatta tgtcgtccgt atggagggag gcgcgtttaa tggttatccc
     4561 gccgatgctc acgggctcca ggtccgcgac cggagtcaga agcccgaccc gcccgacctg
     4621 gacgattatg tcctttatct tcgtcgttcc ctgcctcggc ttgaacttgt aggcgatgtt
     4681 ccatctcggg tgtttcgcgg tcgtcccgag ctccttctga tagttcctac ggtttacctt
     4741 tacgactatg ccgtccgctt cgtaagggag gttgtccctc gctgactcca tctccttctg
     4801 gtaggagata gcttcgtcga tattggcgca agtcatcatg tggtcctcga ccttgaaacc
     4861 ccagtcgcgg agcctctcga cgagatccca ttcggtcttg agctcgatcc ctctcacttc
     4921 acccactccc cacccgtaga acgtgagtgg tctcgacgcc gtgatggcgg agtcgagctg
     4981 ccttatagcg cctgaagccg cgttcctcgg gttggcgaag agcggctcgc ccgcttcccc
     5041 gagctccttg ttcagctttc tgaacgactc gatcgggtag agcacctcgc cccttatctc
     5101 tataagatcg ggaattccct tcttcccttt aagctttagc ggtatcgttt ttatggtttt
     5161 caggttattg gttacgtcct cgccgacgag cccgttcccg cgggtggccc ctttcgtcaa
     5221 aagtccgccg tcgtatgtga gggacgccga cacgccgtcg aacttgggct cggctacgta
     5281 ctcgatatcg ccgtcggtcc cgaggagtct cttcacccgc ttatcgaact cgtatgcgcc
     5341 gtctataccg gtaatattgt caatgctcat cataggcacg atgtggctca cggaggcgaa
     5401 cccttcggcg acccagccgc ccacgcgctg cgtgggagag tccggcttca cgaggtccgg
     5461 gtactccgct tcgagctcct ttaactcgtt cagcatcgaa tcgaattcgt agtcggatat
     5521 ctctggggcg ttcttaacgt aatagagata gttatggtat tcgagctccc tcgaaagctt
     5581 ctctatcttc ttctcagcat ctttctttgt cagtttcata tatttgtcat tctgaacttg
     5641 tttcaggatc tagtctttaa atcttgttgg tctttgttat tacctcggtg gattgaatca
     5701 aaatttgcta cttcctcatc gcattgagct tctctttgag ctcctcatac ggcagggcat
     5761 ttagcacgtc cttcttcctg acccatcccc tccttgccgt gcccacgccg tacctcataa
     5821 acagcagctg ttccgtgctg tgagcgtcag tcgatataat tacctttacc cccgcgtcta
     5881 tggcctttct cgtgtttata tcgttcagat cgagtctcca gtatgagccg ttcacctcga
     5941 gcgcctttcc gtattcaagc gcggcctcga tcacgaggtc taggtccacg tcgtaggggt
     6001 ccctttcgcc gatgagcctc ccggtcgggt gtcctatcgc gtggacgtac gggttcttta
     6061 cggcccttat gatcctgtcc gtcatggtct tcctgtccat cttgaagccg ctgtgcacgg
     6121 acgctatcac aacgtcgagc tccttaagca cgctgtcggg ataatcgaga gtgccgtcac
     6181 tctttatatc gacctccgtg cccatgatta cctttatgtc cctgaacttt tttgcgacag
     6241 catccagctc cttctgtttt ttcctcaatc tctctacgga gaggccgttc gcgatcgtct
     6301 gggacggcga atggtcggag atcgcaatat actcgtatcc gatctctctc gcccgctccg
     6361 ccatctcttc tattgtcgcc tttccgtcgc tccatttcgt atgcgtgtgc aggtcgccct
     6421 ttatgtcccc gagctcgatg aggtcgggca gcttaccctc gatggccgtc tctatctccc
     6481 ccctgtcttc cctgagctcg ggcggtatgt aggggagacc gagcgtcttg tacatctcct
     6541 tctccgtctc
//
LOCUS       DASSJH010000393         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_311490, whole genome shotgun sequence.
ACCESSION   DASSJH010000393 DASSJH010000000
VERSION     DASSJH010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311490"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(504..>2024)
                     /locus_tag="VFJ67_08675"
     CDS             complement(504..>2024)
                     /locus_tag="VFJ67_08675"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7290496.1"
                     /translation="NWIEFSYSRYGRCALLAAKIRKYGLFINGKESDSVSGKTYLREN
                     PATEEPFAEIAEAQKEDVDNAVKAAGKAFEKWSKVPAAERLKLIRRIAELLEGNREKI
                     ALTNTLETGKPIRESRLVEVGGSVKTLEYYTGTATRLNGESISVSEHQLTVTLREPLG
                     VVAQIIPWNFPLLLSLWKIAPALAAGCTIVLKPAEHTPCGILEVAALCSEAGLPEGVL
                     NVVPGMGEVAGQAVVEHPGVAKVAFTGSTEVGKIVMKTAADTIKRISLELGGKAPCIV
                     FGDADIENTIEATLRGGFFNQGENCTAVTRLLLHEDIYDKFLPPFIDRVKKIRQGDPL
                     LEETELGSLISKEHFENVKSYFDKGVDEGGQPLHGGGRPADMEKGYFFEPTVIADVEP
                     DSTVACEEIFGPIVAVIPFKTEEEAVKIANDTVYGLAGGIWTRDIKRALRVAKAVKAG
                     YLWVNTYGGIVPETPYGGFKQSGIGKELGTDGLDMYLETKAVNIYIGDKIPGWYKG"
BASE COUNT          384 a          600 c          526 g          514 t
ORIGIN      
        1 acggaaagta tcggacgagc gtcgagggcg ttttcgccgc cggtgacgcg aggcgcggtc
       61 agagtctcgt agtatgggct ataaacgaag ggcgccaggc cgcgcgcgag tgtgacaggt
      121 acctgatggg ttcgacgaac ctcccgtaat acttttttat aagatgttaa ataattcgga
      181 gctacctttc ccgacattac agaattggcg ttaagaaaat cagctgttaa cgcgttaaga
      241 aatctttcag ggccgcgggt tgaattgaaa gattttagcg ttaacttgcg atttttagcc
      301 gattctgtcc tgtcggatga tttttgcttc ttttcatcaa ggaaaagaag aaaacatatt
      361 ttgccttctt tatgatgtat tccaagttct taaacaagtt cgagacagac acctttcgat
      421 ataatcaaac attccgaccg gccgaactcg atattaagtt cctgcgcgtc atttagtgcg
      481 gactccaggc gagcgggttc tgtttatccc ttgtaccacc ccgggatctt gtcgcctatg
      541 tagatattaa cggctttcgt ttcgagatac atatcgaggc cgtcagtgcc gagctccttc
      601 cctatcccgc tctgcttgaa cccgccgtaa ggcgtctctg gcactatacc gccgtatgta
      661 ttgacccaca ggtatccggc cttcaccgct ttggccaccc tgagggccct ttttatgtcc
      721 ctggtccata taccgcccgc gagcccgtaa acggtgtcgt tggcgatctt cacggcctct
      781 tcctcggtct tgaacggtat gacggccact atggggccga atatctcctc gcacgcgacc
      841 gtcgaatcgg gctctacgtc cgctatgacc gtgggctcga agaagtaccc cttctccatg
      901 tccgcaggac ggcctccgcc gtggagcggc tgtccgcctt cgtccactcc cttatcgaag
      961 taggatttca cgttctcgaa atgctctttc gatataagtg agccgagctc ggtttcctcg
     1021 aggagcgggt ctccctgcct tatcttcttt accctgtcta tgaagggcgg caggaactta
     1081 tcgtatatgt cctcgtgcag gaggagccgc gttacggccg tgcagttctc gccctggttg
     1141 aaaaacccgc ctctcagtgt ggcctcgatc gtattttcga tgtccgcgtc cccgaacacg
     1201 atacaaggcg ctttgccgcc cagctcgagt gatatgcgct ttatggtgtc ggcggctgtc
     1261 ttcatgacta tctttccgac ctccgtcgat cccgtgaacg ccaccttcgc gacgccgggg
     1321 tgctccacga ccgcctgtcc cgctacctca cccatgcccg gcacgacgtt gagcactcct
     1381 tcgggcaatc ccgcttccga gcagagcgcg gccacttcaa gaatgccgca tggggtgtgc
     1441 tcggcgggtt tgagcacgat cgtgcagccg gccgcaagcg ccggggctat cttccagagt
     1501 gataggagaa gggggaagtt ccaggggatt atctgcgcca ccacgccgag cggctccctg
     1561 agcgttacgg tgagctggtg ctccgatacg gagatgctct ccccgttaag cctcgtcgcg
     1621 gtcccggtgt agtattcgag tgtcttgacc gacccgccca cctcaacgag acggctctcg
     1681 cgtatgggct tgccggtctc gagcgtattg gtcagggcga ttttttccct gttcccttcc
     1741 agcagctcgg cgatcctgcg gatgagcttc aaacgctcgg cggccgggac cttcgaccac
     1801 ttttcaaagg ctttcccggc ggccttgacc gcattgtcca cgtcctcctt ctgggcttcc
     1861 gcaatttccg cgaaaggctc ctccgtcgcg ggattttccc tcaaataggt tttcccggag
     1921 acggaatcag actccttccc gtttatgaac agtccgtatt tccttatctt cgcagccaaa
     1981 agcgcgcacc tcccgtaacg actatacgag aattctatcc aatt
//
LOCUS       DASSJH010000394         4293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1501589, whole genome shotgun sequence.
ACCESSION   DASSJH010000394 DASSJH010000000
VERSION     DASSJH010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501589"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..371
                     /locus_tag="VFJ67_08680"
     CDS             48..371
                     /locus_tag="VFJ67_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET7290497.1"
                     /translation="MPRTIEIFEKVERKLREKPDQFSEVGAVYKFIIEGPEGGTWIID
                     LRKDTLGVRTSDEEAECTVEMLDEHFVDLFTGVLPPESALLSGKIRLSGNVLLAMRFA
                     DLLKR"
     gene            complement(446..1321)
                     /locus_tag="VFJ67_08685"
     CDS             complement(446..1321)
                     /locus_tag="VFJ67_08685"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7290498.1"
                     /translation="MSARPPVIIIGMSRSGTSMLTRMLDDLGLFVGSTVTDNHEAVFF
                     RDLNDWLLTQCSGGLENPGMIKYLLEDKEARALYGDFIRFSMKTPRAVSFLGLGKYLR
                     YRTPAALDIPWGWKDPRNTFTLSFWLDMFPDAKVIHIFRHPLDIINSLRTRRERGLSR
                     LRDRHAGSLFKPFYMLYLVRKFIGGRRVFVDLRGASLGEGFILWEEYMREAREQVARL
                     GERAVEVKYEDFLSSPSETLKALTGFCGLETAEGEIEKAAGEVNKSRGYAFLKDPELK
                     AYSLEIAARLGDYGY"
     gene            complement(1463..2659)
                     /locus_tag="VFJ67_08690"
     CDS             complement(1463..2659)
                     /locus_tag="VFJ67_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7290499.1"
                     /translation="MKSAKFREAERVTPEIGSMENYNEQEEESYSSLGFRDDVLIEGE
                     EADFGFEIEDNTASEDQEEEWTPDEQFRLLYVYFKDMAVESLLTAKEEIEVSAQIKNC
                     ESKTKELTSHIQKLSKKKDSFSGKKKSGNTAITEKELAKRIDSLMAFVKVYSERASEL
                     KERFVKANLRLVVSISKRYMGRGLPLTDLIQEGNVGLMRAVERFDHTKGFKFSTYASW
                     WIHQAISRALLDQTRTIRVPVYVLEQASKVYRISSMLHKELGRKPLPNEVAEVAGISV
                     EGVKRILESTNDAARLDTPILDGEKTTLLDFISDDNSPAPDSVMANTALTHEIRDALN
                     ILTSREEEIIRLRFGIDQDSTYTLDEIGRRFDLTRERIRQIEKRALEKLATSEMGEIL
                     RSFLAR"
     gene            complement(2908..3711)
                     /locus_tag="VFJ67_08695"
     CDS             complement(2908..3711)
                     /locus_tag="VFJ67_08695"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7290500.1"
                     /translation="MRELKPKLMMANSGRRRRGPGGGFWSFLTFIIVFLVGVYVGTKV
                     DDFNLSGTEPVPVKQTKEENDSVKTTVKYETSTEPVISGEDNPPGETTASTSEKFLSL
                     DGKNISEELIKPSPELSINTNDKKGISDSGLTSIDPAQIGGETKTTEEASLAKPENTA
                     PETAKTEEIQPEKTPKRGYTLQVGAFADKARAERAVSEYRSKGFSAYTVQVENSKGET
                     WNLVKIGKYSNIEQAWSQSALFKRAVGKDAFVESLGTKTVFNESWGKDE"
     gene            complement(3731..3815)
                     /locus_tag="VFJ67_08700"
     tRNA            complement(3731..3815)
                     /locus_tag="VFJ67_08700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3778..3780),aa:Leu,seq:cag)
     gene            complement(4002..>4293)
                     /locus_tag="VFJ67_08705"
     CDS             complement(4002..>4293)
                     /locus_tag="VFJ67_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005555278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET7290501.1"
                     /translation="DKKEKSVMPRELKRVRNIMSNPRVSLVVDGYYEDWNRLYFVLIS
                     GHAEVLGSGGEYRNSLGILARKYTQYVKMGLEDAGLPVIKITPEKIVSWGVL"
BASE COUNT          961 a         1161 c         1056 g         1115 t
ORIGIN      
        1 ggagatggta aaatggattc ccattcatcc caaggagcaa agccccattg ccgaggacaa
       61 tagaaatatt cgagaaagtg gagaggaagc tcagagaaaa acccgaccag ttttcggagg
      121 tgggggccgt ttataaattc ataatcgagg gcccggaagg gggaacgtgg attatcgatc
      181 tgagaaagga cacgctcggg gtgaggacat cagacgaaga ggccgagtgc acggtcgaga
      241 tgctggacga acacttcgtt gacctcttca cgggtgttct gcctcccgag agcgcgctgc
      301 tatcggggaa gatcaggctc tccgggaacg tgctcctggc catgaggttc gcagatctgt
      361 taaagagatg agacgcgcgg aagatggaac gcgcatttcc ggagcaggta ttcccgcacc
      421 aagaatcgag gcacatgccg attagctagt aaccgtaatc cccgagcctc gcggctatct
      481 ccagggaata ggctttgagc tcggggtctt taagaaacgc atacccgcgc gacttgttta
      541 cctcgcccgc agctttttct atctcgcctt cggcggtttc gagaccgcag aatccggtta
      601 gagccttgag cgtctcagac gggctgctta gaaaatcctc gtacttcacc tcgaccgccc
      661 gctctccgag ccgggcgacc tgttcccgag cctctctcat gtactcttcc cacaatataa
      721 acccctcacc cagggaagcc cctctcaaat ccacgaacac gcgtctcccg cctatgaatt
      781 tccgcacgag gtagagcatg taaaaaggtt taaacagaga cccggcgtgc ctgtcgcgca
      841 gccgcgaaag gcccctctct ctcctcgtcc tcaggctgtt tataatgtcg agcgggtgac
      901 ggaaaatatg tatcaccttc gcatcgggga acatatcgag ccagaaagag agcgtgaatg
      961 tgttacgcgg gtccttccat ccccagggga tgtcgagagc ggcgggcgtc cggtagcgga
     1021 ggtacttgcc gagcccgagg aacgatactg cacggggggt cttcatcgag aatcttatga
     1081 aatccccgta gagggccctc gcctccttat cctcaagcag gtatttgatc atgccggggt
     1141 tctccaagcc tccgctgcac tgggtgagca gccagtcgtt cagatcgcgg aagaatacag
     1201 cctcgtggtt gtctgtgacc gtgcttccga cgaagaggcc gagatcatcg agcatacgcg
     1261 tgagcatgct cgtgccggaa cggctcattc cgattatgat tacaggcgga cgggcgctca
     1321 tttcaatctg aaattataca acaagcggca tgatggcaat gcctccggcc ggaatagtcg
     1381 attataagag atttataccc gggtataaag ttacggattg ccgtcatgcc ttaagcagta
     1441 acagactcgt tggagcagcc ggtcagcggg cgaggaagct tctcagaatc tcgcccatct
     1501 cggacgtggc gagcttctcg agagcccttt tctctatctg tcttatcctc tcgcgcgtga
     1561 ggtcgaacct cctgccgatc tcgtcgagag tgtaagtgga atcctggtcg attccgaacc
     1621 tgagccttat gatctcttcc tctctggacg tgaggatatt gagcgcgtcc ctgatctcgt
     1681 gcgtgagagc cgtattggcc atgacggagt cgggcgcggg agaattatcg tcggatataa
     1741 aatcgaggag cgtcgtcttc tcgccgtcga gtatcggggt gtctaggcgc gcggcgtcgt
     1801 tcgtagactc gagaatcctc ttaacgcctt cgaccgatat gcccgcgacc tcggccactt
     1861 cgttgggcag cggtttcctc ccaagctcct tatggagcat ggagctaatc ctgtaaacct
     1921 tgctcgcctg ctcgagtacg taaaccggga cgcggatcgt tctcgtctga tcgagaagcg
     1981 cccttgagat tgcctgatgt atccaccagg aggcgtatgt cgagaacttg aatcccttgg
     2041 tatggtcgaa cctctcgacg gccctcatga gcccgacgtt gccttcctga atcaggtcgg
     2101 ttaggggcag ccctcgtccc atgtaccttt tcgatatact gacgacgagc ctgaggttag
     2161 ccttgacgaa tctttccttg agctcgcttg cgcgctcgga atataccttc acgaacgcca
     2221 tgaggctatc tatacgcttc gcaagctctt tttcggtgat tgcggtgttc ccgctctttt
     2281 tcttccctga aaagctgtcc ttttttttgg agagtttctg gatatgagac gtgagctctt
     2341 tcgtttttga ttcacaattc ttaatctggg cggagacctc tatttcctcc ttcgccgtaa
     2401 ggagagattc gaccgccata tccttaaaat agacataaag aagcctgaac tgctcgtccg
     2461 gagtccattc ttcttcctga tcttcggaag ccgtattgtc ttcgatctcg aacccgaagt
     2521 cagcctcctc tccctcgatc aggacgtcgt ctctgaaacc gagagaagaa tacgattcct
     2581 cttcctgctc gttatagttt tccatgctcc cgatctcagg agtcacacgt tccgcttctc
     2641 tgaatttcgc agatttcatt ctcacaccta tccagaccct ttatatgtat ttgataattg
     2701 taacgctaat tagtgctgac agtgccctaa ctatattaat atacggattc gaagattcct
     2761 tgtcaagaaa tttcttcgca attgcaaaaa tgctaaagtt atatgtatta ttcaataata
     2821 acggggtgtc tcgatgcaga atcaaccgca gcatacgcat aagctaacag agcatagcac
     2881 gcagttatat gtagaatata aatccggcta ctcatctttt ccccacgatt cgttgaagac
     2941 ggtcttcgtt ccgagagatt cgacaaaggc atccttacct acggcgcgct tgaaaagggc
     3001 cgattggctc catgcctgct ctatgttgct gtatttacct atttttacga ggttccaggt
     3061 ctcacccttg gaattctcca cctgaaccgt gtaggcgcta aaccctttgc tccggtactc
     3121 gctcaccgcc ctctcggccc gtgccttatc ggcgaaagct cccacttgga gagtataccc
     3181 tcttttgggt gttttctcgg gctggatttc ttcggtcttt gccgtctcgg gagccgtatt
     3241 ttccggcttg gcgagggacg cttcttccgt ggttttcgtc tccccgccta tctgagcggg
     3301 gtcgatggat gtaagtccgg aatcgcttat ccccttttta tcattagtgt ttattgacaa
     3361 ttcgggcgaa ggcttgatta attcttctga aatatttttt ccgtccagcg acaaaaactt
     3421 ttcactcgtg gaagcggttg tttcgccggg agggttgtct tccccggaga ttaccggctc
     3481 cgtggaagtt tcatatttta ccgtggtttt tacgctgtcg ttctcctctt ttgtctgttt
     3541 taccggaacg ggctcggtgc cgcttagatt aaaatcgtca accttggtgc ctacatatac
     3601 gccaacaagg aatactataa tgaacgtgag gaaagaccag aaccctccgc cggggccacg
     3661 tcttctcctg cccgagttgg ccatcataag ctttggctta agctctctca taatttatct
     3721 aaaaagtcca tgccgaaggg gggatttgaa cccccacgag ctcgcaccca ctggatcctg
     3781 aatccagcgc gtctaccaag ttccgccact tcggcgatat agaatttgaa taacccgaaa
     3841 cccttaatcg ataatattat catagataat catggcatgc tagggttttt gagataataa
     3901 gtacccgcac tatcccttta tcattacttt tttcaaaacg gagccgacaa ccctgacagc
     3961 atctttccgg aagctccgag ccgtaaagcc gagctcaaga ttcagagtac gccccaagaa
     4021 acaattttct caggcgttat tttaatgacc ggaagacccg catcctcgag acccattttt
     4081 acgtattggg tgtatttccg ggctaatatt ccgagggaat tccgatactc cccgccgctt
     4141 ccgaggacct cggcatgacc ggaaatcaat acgaaataaa gcctgttcca gtcctcataa
     4201 taaccgtcca caacgagcga tacccgcggg tttgacatga tattcctgac acgcttcaat
     4261 tcccggggca tgacgctctt ctccttctta tcg
//
LOCUS       DASSJH010000395         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_628345, whole genome shotgun sequence.
ACCESSION   DASSJH010000395 DASSJH010000000
VERSION     DASSJH010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628345"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFJ67_08710"
     CDS             complement(<1..347)
                     /locus_tag="VFJ67_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290502.1"
                     /translation="MKKMSLDVERTGLDISEKGRAKDGTELSSDRRLLMQLLAFGNCH
                     NTRKLVDELKKSKIDFALYADINDPYGIGLLAVNEDPDFFVGQLREFLNGSSFSELTP
                     KPEYTMLGRTYSFG"
     gene            514..1395
                     /gene="argB"
                     /locus_tag="VFJ67_08715"
     CDS             514..1395
                     /gene="argB"
                     /locus_tag="VFJ67_08715"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET7290503.1"
                     /translation="MKNLIQKAKTLVEALPYIKEFYGQTIVIKYGGSIGDDDLPNFAR
                     DIVLMKFVGIDPVVVHGGGPQIGSHLKKLGIESKFIAGLRVTDEETMEVVEMVLVGKI
                     NQKIIEAIHAMGGKAVGTSGKEGKMILARKLDVKKFALENGLDIPEDADLGMVGEVES
                     VNPNLIDVLESSGFIPVIAPIGFDEDGRTYNINADHVAGKIAGALKAAKLILMTNVPG
                     IMDNEKNLLSSITEAQARHLIKKEIISSGMIPKVMCAIEALHDGVHKVHIIDGTIEHS
                     LILEIFTDAGIGTEILL"
     gene            1392..2594
                     /locus_tag="VFJ67_08720"
     CDS             1392..2594
                     /locus_tag="VFJ67_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7290504.1"
                     /translation="MKTKEIISGTSEYLMNTYGRYPIAMVRGKGSWVWDSEGKKYLDF
                     VTGIAVNNLGHCHPNVVRAISEQSRKLVQVCNLYHIDRQVELAQMLVENSFADKAFFC
                     NSGAEANEGAIKLARKWGEAHGGRFKIIHARDSFHGRTMGALSATNKKYQKGFRPLVP
                     GFKPVPYGRIEPIEKALRDEKVCALILEPIQGENGVIIPYANFLRDARRLCKSKNVLL
                     ILDEIQVGMGRTGKLFAYEHHGVKPDVMTLAKALGGGIPCGAILATDQVAKHFSPGNH
                     GTTLGGNPLAMSAGIAVLKTIIEDGLLDNAAGMGEYFLSGLVNLRDEFGSLIRDVRGK
                     GLILGVEFTGGEAAKDAVNRCMNNGLLTLLTEQKVMRILPPLNVRKSEIDQALRIVRK
                     SLKEVKSA"
     gene            2587..>2804
                     /locus_tag="VFJ67_08725"
     CDS             2587..>2804
                     /locus_tag="VFJ67_08725"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012925.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HET7290505.1"
                     /translation="MPRHFLRISDITKKELNSLFKRASKLKAEKKSGRMRASLRGKSI
                     GLVFEKLSTRTRVSFEVAINDLGANSLYL"
BASE COUNT          748 a          712 c          804 g          540 t
ORIGIN      
        1 ccgaacgagt aagtcctgcc gagcatcgta tattcaggtt tcggggtaag ctcggagaat
       61 gacgatccgt tcaggaactc cctcagctgg ccgacgaaga agtcagggtc ctcgttcacg
      121 gccagaagcc ctatgccgta ggggtcgttt atatcggcgt aaagggcgaa atctatcttt
      181 gactttttca attcatcgac taatttccgg gtgttatggc agtttccgaa ggcgaggagc
      241 tgcatgagca gccttctgtc cgaggagagc tctgttccgt ccttcgccct acccttctcg
      301 cttatatcga gacccgtgcg ttcaacatca agagacattt tcttcatacc tccgcggtta
      361 ttaaatatat cagggaaacg ggcggttggc gattgaaggg gatcatttgc gcccgctccc
      421 gccaattatc cgcgcaataa aaagaattta tttgcatgga atcgtattta ttttataata
      481 atcaccttta ttttcagtct gtaaaagagg cggatgaaaa accttatcca gaaggctaaa
      541 acgctcgtag aggctctgcc ctacataaag gagttctacg ggcagacgat agtcataaag
      601 tacggcggga gcatcgggga cgacgacctg ccgaacttcg cccgcgacat cgtgctcatg
      661 aaattcgtgg gtatcgatcc ggtcgtggtc cacgggggcg gcccccagat agggagccac
      721 ctgaaaaagc ttgggataga gtcgaaattc attgccgggc tcagggtgac ggacgaagaa
      781 acgatggaag tcgtcgagat ggtgctcgtt ggaaagatta accagaagat catagaagcc
      841 atccatgcga tgggcgggaa ggcggtcggc acgtcgggca aggaagggaa aatgatcctc
      901 gcaaggaagc tcgacgtgaa gaaattcgct ctggagaacg ggctcgacat accggaagac
      961 gcagacctgg gcatggtggg cgaggtcgag agcgtaaacc ccaacctcat agacgttctc
     1021 gagagctcgg gctttatacc cgtaatcgcg cccataggtt tcgacgagga cgggaggaca
     1081 tataatatca acgccgacca tgtggccggg aaaatagcgg gcgctcttaa agcggcgaag
     1141 ctaatactca tgaccaacgt gcccggaatc atggacaacg aaaagaacct cctctcgagc
     1201 attacggagg cccaggccag gcacctcatc aagaaagaga taatatcgag cggcatgatc
     1261 cccaaagtca tgtgcgctat agaagccctc cacgacggag tccacaaggt tcacatcata
     1321 gacggcacga tagagcactc gctcatcctc gaaatattca cggacgccgg tataggtacg
     1381 gagatactgc tttgaaaacc aaagagataa tttccggcac atccgaatac ctgatgaaca
     1441 cgtacggcag gtacccgatc gccatggtca ggggtaaagg ctcctgggtg tgggactccg
     1501 aggggaaaaa gtacctcgat ttcgtgacgg gaatagccgt aaacaacctg gggcattgcc
     1561 atccgaacgt ggtgagggct atatccgagc agtcgaggaa gctcgtccag gtgtgcaacc
     1621 tctaccatat agacaggcag gtcgagttag ctcagatgct cgtcgagaac tctttcgccg
     1681 acaaagcgtt tttctgcaac agcggtgctg aagcgaacga aggggcgata aagcttgcga
     1741 ggaaatgggg ggaggcacac ggcggcaggt tcaaaataat acacgcccgc gactccttcc
     1801 acggaaggac catgggcgcg ctctccgcaa cgaacaaaaa ataccagaag gggttcaggc
     1861 ccctcgtccc gggctttaaa cccgttccat acggacggat cgagcccatc gaaaaagccc
     1921 tccgggacga gaaggtgtgc gccctgatac tcgaacccat acagggcgag aacggcgtca
     1981 tcatccccta tgcgaacttt ctcagggacg cccggaggct gtgcaaaagt aaaaacgtgc
     2041 tcctcatact ggacgagata caggtgggaa tgggaaggac agggaagctg ttcgcctacg
     2101 agcatcacgg cgtaaaaccg gacgtaatga cactcgccaa ggcgctcggc gggggaatac
     2161 cctgcggcgc tatactggcg acggatcaag tggcgaagca cttctcaccc ggcaatcacg
     2221 gtacgacgct cgggggcaat ccgctcgcta tgagcgcagg catcgcggtt ctcaagacca
     2281 tcatcgagga cggacttctc gacaacgccg ccgggatggg agagtatttc ctgagcgggc
     2341 tagtaaatct cagggacgag ttcggcagcc tcatcaggga cgtgagaggt aaagggctca
     2401 tactgggcgt cgaattcacg ggcggggaag cggcaaagga cgcggttaac agatgcatga
     2461 acaacggact gctcacgctc ctcacggagc agaaggtgat gaggattctc ccgccgctta
     2521 acgtcagaaa gagcgagata gaccaggcgc tccggatcgt acgcaagtcg cttaaagagg
     2581 ttaaaagtgc ctagacattt cctcagaata tccgacatca cgaagaagga gctaaattcc
     2641 ctcttcaaaa gggcatcgaa gctcaaggcc gaaaagaagt caggcaggat gcgcgcctct
     2701 ctaaggggaa agtcgatcgg actcgttttc gaaaagttat cgacgaggac cagggtctct
     2761 ttcgaggtgg ctataaacga cctgggagcg aactcgctct acct
//
LOCUS       DASSJH010000396         4469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_203731, whole genome shotgun sequence.
ACCESSION   DASSJH010000396 DASSJH010000000
VERSION     DASSJH010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203731"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFJ67_08730"
     CDS             <1..202
                     /locus_tag="VFJ67_08730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7290506.1"
                     /translation="IVLVPQPEVDYITLAKDLGIYMIPRSDGIMLGGSRDYGNWSMAP
                     DPEVTERIFTRQSEFWSGLPSV"
     gene            217..891
                     /locus_tag="VFJ67_08735"
     CDS             217..891
                     /locus_tag="VFJ67_08735"
                     /inference="COORDINATES: protein motif:HMM:NF008228.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HET7290507.1"
                     /translation="MDSFTQILLGTIISLLPIVNPFSVAVTFISLSKDMEDGKRKKQA
                     LLASVYMAAILIIFLVAGVLIMKFFGISLPGIRIAGGLIITYIGFRMLNPELRDPQIQ
                     AAGTKSADDDIAFTPVAMPMLSGPGAIAVTIGMAANAGSRFEYVGETLGIIVVALITY
                     VLLRIAGSVKHLLGDYGIGILTRILGFLLICIGVQFVMLGFHDFILDESFSRPVLEMI
                     KEVWKK"
     gene            879..1382
                     /locus_tag="VFJ67_08740"
     CDS             879..1382
                     /locus_tag="VFJ67_08740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7290508.1"
                     /translation="MEEVTVEELSIRRYRPEDAEAIAGLYEKSVREIAIEKYTPEAVE
                     AWASYADEIEELRHRLAEGLTLVAEAGDILIAFGQLKPVDHIAFLYTLKEYSRKGIAS
                     GIYKRLEDHAAMKGAEYLTTDASRISKPLFERLGFKLECPVVEKRKGVDIECFKMRKE
                     LRVNKGI"
     gene            1837..4290
                     /locus_tag="VFJ67_08745"
     CDS             1837..4290
                     /locus_tag="VFJ67_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HET7290509.1"
                     /translation="MTNTTATRENSPGEVLPVSRCLHCGEDLDSSNTIESNGKLFCCN
                     GCRSVYELLGSLGLDDYYAIRESQDIIQTTPPADPASGEDYGYLDSESFAGLYVREEK
                     PHTMNFYVEGIECAACLWLIEKIPSFVPEIESLSLNMSDNTAAVNFTAENKFSSFPDT
                     LKKLGYKAHPLKIDEDASGLKRRENRKSLIRLSIAAVCAGNIMLLSAAIYSGARGVFE
                     EQFLILNLILSLPVVTYCSLPFYKSVIGSVRTGRATVDIPIVLVITAGFGISAYNYLS
                     GSPEVYFDSITMFVFLLLASRYFLRSVQDRLNDGELLAKSLFSSNRVLVRDEGAKQFF
                     YQPVEYLEPGRRIKLARGDRFPADGILLSRRAEVNLSVLTGENIPRTVSKGDTVYAGS
                     ILDSDEAVLDVTGTGASTRIGKILEEVESNYKSKISLSTYSDRYATVFTFAVGIIAAV
                     SFFVVSSYYGASEALDRTIAFILISCPCAFVFVMPLSFGLFLKASSENGLLIKDARIF
                     DKLPRVRSIYFDKTGTLTRGVYRILSWDTETLTEADRAAVLAIERESEHPVARAIVSC
                     LSGENLDIPEVSGFRHIYSKGVEGRVSGHLYSLTAEACDTRQNEINEIIATRIVIRKD
                     GEPVSEILLGDSLKEDAKFVVTELKKRGYSLYILSGDRDANVKQVAGKLDIPLGNVFS
                     EKTPEMKSAIVRDMSGSMMIGDGLNDAGALSSSDVGVAIQGSVGESMKVSDAYILEND
                     LFSILDLLDHGRIIRQTLRRNTFFSVSYNVSAGALALAGFINPLAAAVLMPLSSVLLI
                     VSSLYGRNRTRIREGAARI"
     gene            4287..>4469
                     /gene="ccoS"
                     /locus_tag="VFJ67_08750"
     CDS             4287..>4469
                     /gene="ccoS"
                     /locus_tag="VFJ67_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HET7290510.1"
                     /translation="MNIIFLTLGVSIAIALIFLGAFIWAAGRGQYDDLETPGHRMLLD
                     DYESDKKNQDAKEETGK"
BASE COUNT         1109 a         1155 c         1249 g          956 t
ORIGIN      
        1 catcgtgctc gtcccccagc cggaggtcga ctatatcacg ctcgcgaagg acttgggcat
       61 ttacatgata ccgcgctcgg acggcataat gctgggaggg tcgagggact acgggaactg
      121 gagcatggcg cccgaccccg aagtcactga gcgcatattc acgaggcagt ccgaattctg
      181 gtccgggctc ccctcggtct agtgtaaaat taccggatgg acagcttcac acagatactc
      241 ctcggaacga tcatatccct gcttccgatc gtgaacccgt tctcggtcgc agtcaccttc
      301 atatcgttat ccaaggacat ggaagacggc aaaagaaaga aacaggctct tctagcctcg
      361 gtctacatgg ctgcgatact cattatattc ctcgtcgcag gcgtgcttat aatgaagttc
      421 ttcggtattt cgctgccggg aatacgaata gcaggcggct tgatcattac ctatatagga
      481 ttcaggatgc tgaacccgga actgagagac ccgcagatac aggccgcggg gacgaaatcc
      541 gcggacgacg atatcgcatt caccccggtc gccatgccca tgctgagcgg gcccggggcg
      601 atagcggtga cgatagggat ggccgccaac gcgggctcga ggttcgagta cgtgggggag
      661 acgctcggca tcatagtcgt cgccctgatt acatacgtgc ttctcaggat cgcgggcagc
      721 gttaaacacc tgctcgggga ctacggtata gggatactga cccggatact tgggttcctc
      781 ctcatctgca taggggtcca gttcgtgatg cttggtttcc acgactttat cctggacgag
      841 agtttttccc gccccgtgct cgagatgata aaggaggtgt ggaagaagtg actgtggagg
      901 agctgagcat ccgcaggtac aggcccgagg acgccgaagc tatagccggg ctctacgaaa
      961 agtcggtaag ggagatcgct atagagaagt acacgccgga agcggtggag gcgtgggctt
     1021 cttacgcgga cgagatcgag gagctgaggc acaggctcgc cgaagggcta acgctcgtcg
     1081 cggaggccgg ggacatactc atcgcattcg gccagctcaa gcccgtagac cacatagctt
     1141 ttctatatac actgaaagag tattcgagaa aggggatagc gtccggaatc tataagcggc
     1201 tcgaggacca cgcagcgatg aagggggccg aataccttac gactgacgca agccggatat
     1261 cgaaaccact ttttgaaagg ctcggcttca aactcgagtg tccggtcgtc gagaaaagaa
     1321 agggcgtcga tatcgaatgc tttaagatga ggaaggagct gcgggttaat aaagggattt
     1381 aatttttgta atacgataat gaaaccctcc cggtcccgta tcccggggaa aaataaataa
     1441 acgttgattc cgaccgccgg aaagatatat aatcgctttt atagagacgc ttggggaggc
     1501 cgttatgaaa gatacttccg gacaaacatt aggcgcgatc cttttcctca ccgcaatggg
     1561 tgtgtttttc gcactatggt tctatttgtg gttcagctaa gctgaaagcg gattgagccg
     1621 ccaaatccat agaattcctc atagactcgt gtacaatcat ccgtacgcga gacgtattgt
     1681 aatagtatga caaggcgaat aaagtcagct tcatttacgc gttgtcctat tatctgacat
     1741 tagacaccgc ttatagccat gaaatatcgt attcattagt gtttgcagtg gcatagttat
     1801 tgctcgtaac taagttcgaa agaattgtaa actgcaatga ctaatacgac agcgacgaga
     1861 gaaaacagtc ccggtgaggt tctgcccgtc agccggtgcc tccactgcgg tgaagacctt
     1921 gattcatcaa ataccataga atcgaacggt aagctgttct gctgcaacgg ttgcaggtcg
     1981 gtgtatgagc tgctcggctc gctcggcctg gacgactatt acgccatcag ggaatcccag
     2041 gacataattc aaacaacacc ccccgccgac ccggcctcgg gagaggatta cggatacctc
     2101 gacagcgaga gcttcgccgg tctctatgtc agggaagaga agccgcatac aatgaatttt
     2161 tatgtagagg ggatagaatg cgccgcatgc ctctggctca tcgagaagat acccagcttc
     2221 gtccccgaga tagaatcgct gtcgctcaac atgtcggaca acaccgctgc ggttaacttc
     2281 acggccgaaa ataaattctc gtcattcccc gatacattaa agaaactcgg atacaaggcc
     2341 caccctctca agatcgacga ggacgcgagc gggctcaaga gaagggagaa caggaagtcc
     2401 cttatcaggc tgtctatagc agccgtatgc gcaggaaaca taatgctcct gtcggcggcc
     2461 atatactcgg gagcgcgcgg ggtgttcgag gaacaattcc tcatcctcaa tctgattctg
     2521 tcgctcccgg tagtaaccta ctgctccctg ccattctata agagcgtcat aggctcggtc
     2581 aggacggggc gcgctacggt ggacattccc atagtgcttg tcataacggc cgggttcggt
     2641 atcagcgcat acaactatct ctcgggaagc cccgaggtat atttcgattc gataaccatg
     2701 ttcgtattcc tcctcctcgc cagcaggtat ttcctgagga gcgtacagga caggctcaac
     2761 gacggtgagc ttctggcaaa gagcctgttt tcatccaacc gggtactcgt ccgtgacgaa
     2821 ggggcgaagc agttctttta ccaacccgtc gaatacctcg agccgggccg gaggataaag
     2881 ctcgcgagag gcgaccgctt cccggcggac gggatattgc tctcccgcag ggcggaggtc
     2941 aacctctcag tgctcacggg agagaacatt ccccggaccg tctcaaaagg ggatacggtc
     3001 tacgcgggct ccatactcga ctcggacgag gctgtactcg atgtgacggg aacgggggca
     3061 tcgacgagga tcggaaagat tctcgaggag gtcgagagca actacaagag caagatcagc
     3121 ctctccacct acagcgacag atacgcgacc gtcttcacat tcgcggtcgg catcatagcg
     3181 gcagtttcat ttttcgtggt ctcatcatac tacggtgctt ccgaagcgct cgacaggacg
     3241 atagccttca tactaatttc atgcccatgt gccttcgttt tcgtcatgcc tctctcgttc
     3301 gggctcttcc tgaaagcctc ctccgaaaac ggattactca taaaagacgc cagaatattc
     3361 gacaagctgc cgcgagtgcg gagcatatac ttcgacaaga ccgggacgct cacgagaggc
     3421 gtttacagga tactcagctg ggatacggaa acgttaacgg aggccgaccg cgctgcggtg
     3481 ctggctatag agcgggaatc cgaacacccg gtcgcgaggg ctatagtgtc atgcctctcg
     3541 ggagagaacc tcgatatacc ggaggtatcc ggcttcaggc acatctactc gaagggcgtg
     3601 gaaggacggg tctccggaca cctctactcg ctcacggcag aggcgtgtga tacacgtcag
     3661 aacgaaatca acgaaataat agcgacaagg atcgtcatca gaaaggacgg tgagcccgtc
     3721 tcggaaatac tcctcgggga ttcacttaaa gaagatgcga agttcgtggt gaccgagctt
     3781 aaaaaaaggg gctactcgct ctatatcctt tcaggggaca gagacgcgaa tgtaaagcag
     3841 gtagccggca agctggatat tcccctcggg aacgttttct ccgagaagac gccggagatg
     3901 aaatccgcaa tcgtcaggga catgagcggc tcaatgatga tcggggacgg gctcaacgac
     3961 gcaggagcgc tctcttcttc cgacgtcggc gtggcgattc aggggagcgt cggggaaagc
     4021 atgaaggtct cggacgctta tatattagag aacgacctgt tctcgatact cgacctgctc
     4081 gaccacggca ggattatacg ccaaaccctg agaaggaata ctttcttctc cgtatcgtat
     4141 aatgtctcgg ccggggcgct cgcgctcgcg ggcttcataa atcccctggc ggcggctgtg
     4201 ctgatgccgc taagctcagt actcctgata gtctcatcgc tctacggacg taacaggacc
     4261 aggatccggg agggagcggc gcggatatga acattatttt tctcacgctc ggggtctcga
     4321 tagcaatagc gctgatattc ctcggggcct ttatatgggc tgcgggaagg ggtcagtacg
     4381 atgacctcga aaccccgggg cacaggatgc ttctcgacga ttatgaatca gataaaaaga
     4441 accaagatgc taaggaggaa accggaaaa
//
LOCUS       DASSJH010000397         4297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1393333, whole genome shotgun sequence.
ACCESSION   DASSJH010000397 DASSJH010000000
VERSION     DASSJH010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393333"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..883
                     /locus_tag="VFJ67_08755"
     CDS             395..883
                     /locus_tag="VFJ67_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290511.1"
                     /translation="MNYNESLIKRSVELIILLSSIITIAFMITGGCGGGGKKAQPRPT
                     GPPPGCEDIYIDPECPAVSLAGLCRFWGGYFCNFTEINPENPEPETIGLGIQLADCTI
                     LDCFTLECEEIFPPVTEMPKVTLYIMGIEIISDIIPAAFSGTADIDGFGFDYVCNPPP
                     IP"
     gene            972..1853
                     /locus_tag="VFJ67_08760"
     CDS             972..1853
                     /locus_tag="VFJ67_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290512.1"
                     /translation="MKYKSIVGNRTYRDLFYTLRALTFALLVIISAVAISSSAEDNIL
                     GFYGKAVKRADSPPDESLLSVECREKIDNIYVTFVGAEKGKVLLAAITQEKLKKPDKY
                     ILLKVYYTKSLENPILPTRDYVMDWGYVYDRNGDGKIDYVAYNVGPMPFKQKDFPEDY
                     PKGGQSLNLSDIELLFKSMRLLFTHWADDNYDGKVDTVVFEAVDPERNWVEGWTAVRS
                     TKYDGTVDEGWYFKEDINVREKDAERTGTGFKTRRIPGIEPVFGPGELAHKTKILTMF
                     NEAAEKCKLTGDSFYRQ"
     gene            2033..2566
                     /locus_tag="VFJ67_08765"
     CDS             2033..2566
                     /locus_tag="VFJ67_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7290513.1"
                     /translation="MARRQNMFVFAIFTALAFAGILSLGQINARAQLDNNCIISITKA
                     ATPANDTEFSFVTTGIQEGTFFLSDPSHTLQVLGINVQQSITVTEDAIPGWTLDGIEC
                     TEGVINCGSTSCLTATVDGNSVTFECFDNDTASCTFTNSFTGANIPTLSEWGLISMAV
                     ILGIAGIILMRRRRVTA"
     gene            2839..3867
                     /locus_tag="VFJ67_08770"
     CDS             2839..3867
                     /locus_tag="VFJ67_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7290514.1"
                     /translation="MDSPHVFHLLSTKPQVSVKGGTRTYASKSNFPALRGMALYRLIL
                     RERGVREPHWHPNANELAYCLRGNGVVTIFGNYSERETFTVSPGEMFFVPSGYLHHIE
                     NTGKDEMELMLAFSDGEPEDFGLSASLGCMTDAVLGNTWGLKSKSFSKIKRSPKNIVI
                     GMKKSRGEAPEYAYFPSKYKYNVEAAFPLLANDGGWAKVARKNVWPVLEGLSMYSLRI
                     TDKGMREPHWHPVTAEMGYVDRGRARMTILSPGGSVDTYTLERGDLYFVPRAYPHHIE
                     NIGREDLHFLVFFDQSTPGDIGFSGSVRAYSNEVLGATFDTDPKTFEMLPDYHEDLLI
                     VSRVNPRD"
     gene            4052..>4297
                     /locus_tag="VFJ67_08775"
     CDS             4052..>4297
                     /locus_tag="VFJ67_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290515.1"
                     /translation="MNAAHWHLILNHIPLIGIGFVILLMIIAFARKSQELKNVAMIFA
                     VIVALWAIPAYLTGEPAEEVVEDMPGISENIIEEHEEF"
BASE COUNT         1126 a         1060 c         1133 g          978 t
ORIGIN      
        1 aaataccggg gatgtaatta tctgcgatgc cgaattcacg ggagaattcg ccggcgaagc
       61 aaaaagtccg gagggctgcc tgagcaatgc cgaattaact ctggatgaga atagagcttg
      121 tcctgcggag tcaatttcca ggatgtgcga taatgtttcc agactttgtc ctatccctgt
      181 tccgtcgccc gccccgtttc caaccccgcc tcccggagat tttcgtattg acaatacttg
      241 tgaagcgtcc ggctgcctta cagtaacaag ttgcagtttc gctccaatac tgacaaattt
      301 ggtgattgga ccgaacggcg gaatctcggg caacctcttt tcaccggacg gcgaacactt
      361 cgcctgtttt tgatggatgc gaggaggaat aaatatgaac tacaacgaat ccctaattaa
      421 gcgttcagta gagctgataa tattactttc atctatcata actatagcat ttatgataac
      481 aggcggatgt ggtggcggcg gtaaaaaagc tcagccccgt ccgacgggtc ctccgccggg
      541 ctgtgaagat atctatatcg acccagagtg tcccgcagtg agcctggccg gcttatgcag
      601 attctggggc ggttactttt gcaattttac cgaaatcaat cccgagaatc ccgagcctga
      661 aacgatcggt ctgggaatac agcttgcgga ttgtacaatt ctggactgct tcacgcttga
      721 atgtgaggaa atatttccgc ctgtgacaga gatgccgaaa gttactctct atattatggg
      781 aatcgaaatc atttctgaca ttataccggc tgcattcagt ggaacagcag atatagacgg
      841 atttggattt gattatgttt gtaaccctcc gccaataccc taacataata atgtttatag
      901 cgggcattgc ttcaacacta taaaagtatt atgagcagag tagaatgaca gtcaggtttc
      961 gggggtgaaa aatgaaatac aagagtatag tcggcaatag aacttatcga gatttatttt
     1021 ataccttgag ggccctgacg ttcgccttac tcgtaattat ttccgcagtc gcaatttcct
     1081 caagcgccga ggacaatatc ctcggcttct acgggaaggc ggtgaaacga gcagactcgc
     1141 cgcccgacga atccctattg tctgtggaat gccgggagaa gatagacaac atatatgtaa
     1201 cttttgtcgg ggccgagaag ggaaaggtcc tccttgcagc tatcacgcag gaaaagctca
     1261 aaaagccgga caaatacatc ctgctaaagg tttattatac aaaatcgctc gaaaacccta
     1321 tcctgccgac aagggattat gtaatggact ggggctatgt gtatgaccgg aacggcgacg
     1381 ggaagatcga ctatgtggca tataatgtcg ggccgatgcc ttttaaacaa aaggacttcc
     1441 ccgaggatta tccgaagggc ggccaatcgt tgaacctatc cgacatagaa ttattattca
     1501 aaagcatgcg gctcttgttc acgcactggg ccgacgacaa ctatgacggc aaggtggaca
     1561 ccgtcgtctt tgaagcggtc gatcccgaaa ggaattgggt cgaaggctgg acggcggtga
     1621 ggagcacgaa atacgacggc acggtcgacg aaggctggta tttcaaagag gatatcaatg
     1681 tcagggaaaa ggacgccgag cgcacaggca ccggattcaa aacccggagg atacctggaa
     1741 tagaacccgt cttcggcccg ggcgagctgg cgcataaaac caagatcctg acaatgttca
     1801 acgaagctgc cgagaagtgc aagctcacgg gcgactcctt ttacaggcag taatcaggat
     1861 tgatatgatg gaaatagaca atcggggaca gttattttgc taccgggttc atctgtaccc
     1921 ccttccctac cgtaatctgc tggtaccttt ccctccgtaa aatttacttg gttgagcgga
     1981 tatatgtgtt agactggcag ataaaagtca ttatcaaatg gaggaaggta atatggcaag
     2041 aagacagaat atgtttgttt tcgcgatatt tacggcgctc gcgtttgccg gcatcctttc
     2101 gctcggccag attaacgccc gggcccagct cgataataac tgcatcatat cgatcacaaa
     2161 ggccgctacg cccgcaaacg acacggaatt ctcctttgtc acgaccggaa tccaggaagg
     2221 cacttttttc ctgagcgacc cgagccacac gctgcaggtt ctcggaatta acgtacagca
     2281 atcgatcacc gtaactgaag acgccatccc gggatggacg ctcgacggca tcgagtgcac
     2341 ggagggagtg atcaattgcg gaagtacgtc ctgcctcacg gctactgtgg acggaaacag
     2401 cgtcacattc gagtgcttcg acaacgatac ggccagctgc acgtttacaa acagcttcac
     2461 gggcgcaaac ataccgacac tttccgaatg ggggttaatt tcgatggcgg tcatactcgg
     2521 catagcaggg atcattctca tgaggagaag aagagtaaca gcttaaggtt taacatatgg
     2581 cggctcatga tataaaagcg ggatcgctac gactacattg aaaaaaatga gatgctgaaa
     2641 cgagttcagc atgacaaaaa tgtttaatgt acaccctcac cctaacccta accctctccc
     2701 ctcaagggag agggaagttg tttgttaggc ggaggaggtt cactagaatc gtatatgagc
     2761 acacattgtg tgctttttcg ttcagcggcg ggggttattg atgtataatt cgtgaaaccg
     2821 taatgaagga ggtttcacat ggactccccg cacgttttcc acctgctctc gacgaaaccc
     2881 caagtttcag tcaaaggcgg gacccgcact tacgcttcaa aatccaactt ccccgccttg
     2941 aggggaatgg ccctttacag gctcattctc cgggaaaggg gcgtccgcga gccccactgg
     3001 cacccgaacg cgaacgagct cgcatactgc ctcagaggaa acggggtcgt caccatattc
     3061 ggcaattaca gcgagagaga gacgttcaca gtctcgcccg gtgagatgtt cttcgtcccg
     3121 tctggatacc tccatcacat agagaacacg gggaaggacg agatggagct gatgctcgcg
     3181 ttctcggacg gagagcccga ggacttcggc ctctcggcct cgctcggatg catgacggac
     3241 gcggtcctcg gcaacacctg ggggctcaag agcaagagct tctccaagat taaaaggtct
     3301 ccgaaaaata tcgtcatagg gatgaaaaaa tcccgcggcg aagcgcccga gtacgcgtac
     3361 ttcccgagca agtacaaata caacgtcgag gccgcgttcc cgctccttgc gaacgatggg
     3421 ggatgggcga aggtcgcgag aaagaacgtg tggcccgtgc tcgaagggct atccatgtat
     3481 tcgctccgca taacggacaa ggggatgagg gagccccact ggcaccccgt cacggcggag
     3541 atgggctacg tcgatagggg cagggcgagg atgaccatac taagcccggg cggctcggta
     3601 gatacataca cacttgagag gggcgacctg tatttcgtcc ccagggcgta cccgcaccac
     3661 attgagaaca tcgggaggga agacctccac ttcctcgtct tcttcgacca gagcactccg
     3721 ggggatatag ggttttcggg gagcgtcagg gcgtactcga acgaggtgct gggggcgact
     3781 ttcgacaccg accccaagac tttcgagatg ctgcccgatt atcacgaaga cctgctcatt
     3841 gtgtcgagag tcaacccgag ggattaggct caggccattc ctgcgttcgc gcatacccga
     3901 agtccgatgt cagatgtact gatttctgac acgtgctttt aatcaagagt tccccttttt
     3961 ccatttaatc attggataat cgcttccggc atggttattg caaccctcta acgagaatct
     4021 atcattcact tttttgataa cggaggtcaa aatgaatgcc gctcactggc atctcatatt
     4081 aaaccacata ccgctcatag gaatagggtt cgtgatactg cttatgatta tagcgttcgc
     4141 gaggaagtcc caggagctaa aaaacgtggc catgatattc gcggtcatag tggctctatg
     4201 ggcgattccc gcgtatctca ccggggagcc ggccgaggag gtagtcgagg atatgccggg
     4261 gatatccgag aacatcatcg aggagcacga ggaattc
//
LOCUS       DASSJH010000398         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1278424, whole genome shotgun sequence.
ACCESSION   DASSJH010000398 DASSJH010000000
VERSION     DASSJH010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278424"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..683
                     /locus_tag="VFJ67_08780"
     CDS             240..683
                     /locus_tag="VFJ67_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET7290516.1"
                     /translation="MKAKASDANNSEDLKALFHSKKLKCTPQRLAVLKALRESGAYMS
                     INQLDRKVKEMLPGTGLATVYRALETLEELRLVVKVHLEDGCHSYAVAPEGHMHPVVC
                     TECSRVIEFAECPLGELSEKLTQDTGILIENHFLQLFGKCRECRA"
     gene            700..1410
                     /locus_tag="VFJ67_08785"
     CDS             700..1410
                     /locus_tag="VFJ67_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HET7290517.1"
                     /translation="MSHMHIPDGVLPVWLVTAGWLAAALILAFCIKKVDGTDLKRKIP
                     LLGIISALMIVGMTLEIVPIAYHINLTVVAGIVLGPSLAFIAVFIVDLLISMLGHGGV
                     TVVGLNTIIIGAEAVLGYYLFQLFRQVMRNKSMFWESSLAALLSLFLSTSIMLGVVYL
                     SHVSPEKIGNVGHLISGAGGGTDALHNAGSGINFPRFAKTVFILSPLGWILESAITGL
                     VIKYVFRVRPDLIMQERA"
     gene            1421..2245
                     /locus_tag="VFJ67_08790"
     CDS             1421..2245
                     /locus_tag="VFJ67_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET7290518.1"
                     /translation="MDVSSIDYYANHGTSFLHGARAISKIVFAALVIASVVISGSFYL
                     LLAVYISLLTLVVWTRLPVLKIVTIAAYPAIFALIFAAASWNGSWMSAGVIVLKALSA
                     SIAMVILIVTTPYPDVFSSIGPVLPKIVTEGLFLTYRSLFVLLELTDNLVKGLRVRGG
                     LTHGRYVKNVVNFSSGIGLLLVRGLDLSEKFYGVLKVRGYSGRISAGLRDEKTGANDL
                     TAIVIGALIFGAAIAVKTDSEFSAYDVFMPAVSLLILIVSAAFAYISETRGIAWKR"
BASE COUNT          547 a          601 c          653 g          570 t
ORIGIN      
        1 aagcatcaag aattgttttt taggaattgt tgattaattg actagatatt ttctcgtgtt
       61 aatagatgag attattatac acgagaatac ataagttgcg caaatccggc gattttttcc
      121 cgatcccaac aaccattccg acatatcttc cttgcatcgt gagaatttga cagtatattg
      181 ctatgcgcat ataattgaga atatttctca attaccaaat ccatggataa ggtaaaacaa
      241 tgaaggctaa agcgagcgat gccaacaatt cggaagacct gaaggctctt tttcactcta
      301 aaaaactcaa gtgcactccc cagaggcttg cggttctaaa ggcgctcagg gagagcgggg
      361 cttacatgtc gataaaccag ctcgacagga aggtgaagga gatgcttccg ggtacggggc
      421 tcgcgacggt gtacagggcg ctcgagaccc tcgaagagct caggctcgtg gtcaaggtgc
      481 atctggaaga cggctgccac agctatgccg tcgcgcccga agggcacatg cacccggtag
      541 tgtgcactga gtgcagcagg gtgatcgaat tcgcggagtg cccgctcggg gaattgtccg
      601 agaaactgac ccaggacacc ggcatcctga tcgagaacca tttcctccag cttttcggga
      661 aatgcaggga atgcagggcc tgaaagagga aataaatata tgtctcacat gcacataccc
      721 gacggggtgc tccccgtctg gctggtaacg gcgggatggt tagcggccgc gctgatactc
      781 gcattctgta taaagaaggt cgacggtacc gatctcaaaa gaaagattcc gctcctcggc
      841 ataatctcgg ccctcatgat agtaggcatg acgctcgaga tagttcctat cgcttaccac
      901 atcaacctga ctgttgtggc cgggatcgtg ctcggccctt cgctcgcgtt catcgcggtt
      961 tttatcgtcg atctgctgat ctcgatgctc gggcacggcg gcgtgacggt cgttggactg
     1021 aacactataa tcataggggc cgaagcagtg ctcggctatt atttatttca attgttcagg
     1081 caggttatga ggaataaatc catgttctgg gaatcctcgc tcgcggcgct cctgagcctt
     1141 ttcctgagca cttcgataat gctgggggtc gtttatctat ctcacgtcag ccccgaaaag
     1201 atcggtaatg taggtcattt gatttcgggg gcgggcggcg ggacggatgc gctccataac
     1261 gccgggagcg gaatcaactt cccgaggttt gcgaagaccg tcttcatatt gagccccctc
     1321 ggatggatac tcgaatcggc cataacgggc ctcgtcatca aatatgtatt cagggtgagg
     1381 ccagacctga tcatgcaaga gagggcatag gggttcattc atggacgtct cgtcgataga
     1441 ttattacgct aaccacggca cgagcttcct ccatggggcg cgcgccatct cgaagatcgt
     1501 tttcgcggcg ctcgttatcg cgtcggtcgt catctcgggc agtttctacc tgctgctcgc
     1561 ggtctatatc tcgcttttaa cgctcgtcgt ctggacgagg ctccccgtat tgaagatcgt
     1621 cacgatcgcg gcatatcccg cgatatttgc gctcatattc gcggccgcga gctggaacgg
     1681 cagctggatg agcgccggag ttatagtgct caaggccctc tcggcctcga tcgctatggt
     1741 aatactcatc gtcactaccc cttatcccga cgtcttttca tctatcgggc ccgtcctgcc
     1801 gaagatagtc accgagggcc tgttcctcac atacaggtct ctattcgtcc tcctcgagct
     1861 taccgacaac ctcgtcaagg gactccgcgt aaggggcggg cttacgcacg ggagatatgt
     1921 taaaaacgtc gttaatttct cctccgggat cgggctcctg ctcgtcaggg gtctagacct
     1981 ctcggaaaaa ttctacggag tgctgaaggt aagaggttac agcgggagga tttcagccgg
     2041 tttgagggac gaaaagacag gggcgaacga cctaacggcc atcgtcatag gagctttgat
     2101 attcggagcc gcgattgcgg ttaaaacgga cagtgagttt tcggcgtatg acgtcttcat
     2161 gcccgccgta tctcttttaa tcctgatcgt ctcggccgca tttgcctaca tctctgaaac
     2221 ccgggggata gcgtggaaaa gatagtaagg gtcagggacc tgaaatttaa ataccccgac
     2281 gggacgacgc tcgaattcga cggacacgag tttacagtcg gaagagggga gcgcgttgtc
     2341 atgctagggc ctaacgggag cgggaagtcc a
//
LOCUS       DASSJH010000399         5096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1502401, whole genome shotgun sequence.
ACCESSION   DASSJH010000399 DASSJH010000000
VERSION     DASSJH010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502401"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..714
                     /locus_tag="VFJ67_08795"
     CDS             187..714
                     /locus_tag="VFJ67_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7290519.1"
                     /translation="MNFVKSFIILICFFILGSVIFIYSGTYNVAATVPHSEIATWLFN
                     TVKTSSVRKHSEVIKEPPLDDEAFVREGFEHYEEMCSGCHGAPGSDPAKEFNPAPPAL
                     SETAQEMRPAEIFWIVKNGLKMTGMPESGSTHSDDEIWGIVAFVTRLPGMTPEEYKLM
                     KQNAAKESGHHHEHD"
     gene            786..1205
                     /locus_tag="VFJ67_08800"
     CDS             786..1205
                     /locus_tag="VFJ67_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRASH domain-containing protein"
                     /protein_id="HET7290520.1"
                     /translation="MNSGISRTISLLITAHVLTAAVVIYAGVSAAQNSDSSPAQMNAA
                     QPLKQVESKYVCMVTNQLYDKEQIPVAVGDKTYYGCCQMCEAKLKEDPSSRTAIDPVS
                     GKEVDKSTAVIGASADGKVYYFESVEDLTAYGKTANQ"
     gene            complement(1255..2964)
                     /locus_tag="VFJ67_08805"
     CDS             complement(1255..2964)
                     /locus_tag="VFJ67_08805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET7290521.1"
                     /translation="MRLHDEEVERFRALLDYNILDTPPDPAFDELARLAAYISKSPIA
                     LITFVDETREWFKSVIGVETGLREVPRDKSFGAYVINHPEDVLAVSYPFEDERTKSNP
                     FLHSYQDIKFIAGLPLIDRNGHRIGALCISGFQPKELSFEQVSLLKTIAANVILRLEQ
                     GLGVPGSGGHAAEPVNVSGGAQPDPGIAETDEPSNIHTLTGGRADIMEKIFREIELND
                     DDNSSTIRKDIQALITKLEKRNRKLIQLCEMDELLQASRGEEEVYTIITNYVHKLFPG
                     ETGALLVYNDVLNILECVTSWGDNINSEREFLPDKCWALRLGRLHYVNKAPTELYCQH
                     LIETKDVNYYCVPMLARGKTLGLLYLERNAENAVGIEAEEENTYTSHIVSTMAKLTAL
                     ALGNIMHHQALQNYAIYDSLTGLFNRRYMEETLKREISRVARNKEPLGLIMVDIDHFK
                     HFNDNYGHAAGDMLLRSIGDFFKDRIRREDIACRYGGEEFVLILPGSSLENTCRRAEQ
                     LHEEIKRVRVRHRGTFISSVEVSMGVVVFSEHGTSAELLLESADKALYKAKSQGRNRI
                     VVA"
     gene            3170..3727
                     /locus_tag="VFJ67_08810"
     CDS             3170..3727
                     /locus_tag="VFJ67_08810"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7290522.1"
                     /translation="MSVLNTGTKIGVLVIIFLVLALLGYALFGERETVKTSPLVGKEA
                     PGFTLKLFDGRRVSLEELRGKTVLLNFWASWCMPCRQESQALEESWLKYKDKNVVFIG
                     VNVWDENESAASYIEKFGGGYPQGIDPEEEIQVNYGIGGVPETYFISPAGIITDKYDG
                     PLTEEIIDYYVMKAGGAERETSSMN"
     gene            3738..4232
                     /locus_tag="VFJ67_08815"
     CDS             3738..4232
                     /locus_tag="VFJ67_08815"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HET7290523.1"
                     /translation="MTMKSFQKLNPAALTLTLLLILFFRAGIASPAESLDDRVNEIAH
                     LLMCPVCQGQSVAESNSNLAQDMRQIIRKQLEEGKSKEEVIAYFVNRYGETILASPPP
                     KGVNWLLWLLPALAILFGGLGIGIYLHKTGKGKRSASAKTEKPAADSDYMLKLDEELK
                     KHES"
     gene            complement(4799..>5096)
                     /locus_tag="VFJ67_08820"
     CDS             complement(4799..>5096)
                     /locus_tag="VFJ67_08820"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HET7290524.1"
                     /translation="LRVDRMMEEGLVEEVTGLRRIGYGPELKPMQSIGYKEINRYIDG
                     EISLEKAVELIKRDTKRFAKRQMTWLRRDKEIKWFDIPGDYEKVLDDARAFFGG"
BASE COUNT         1248 a         1278 c         1384 g         1186 t
ORIGIN      
        1 cgataaatgc gaggctgctc aaaatcggga aatgacggca gaatatagac aataatgaaa
       61 atgtttataa taatctcaga gaaaaatgac cgctggataa atcgccggtt aagaattatg
      121 ctagtgaacg tcttttgccg cataatggat caaattgctt gatgtatcat acaagggggg
      181 taacttatga atttcgttaa aagttttata atactgatat gcttttttat tttaggctcc
      241 gttattttta tttattccgg gacatataac gttgccgcca ccgtgccgca ctccgagata
      301 gcgacatggc ttttcaacac ggtcaagacg agctcggtaa ggaaacattc ggaagtcata
      361 aaggagccgc cgctcgatga cgaggcgttc gtcagggagg ggttcgagca ctatgaggaa
      421 atgtgctccg gatgccacgg ggcgcccgga tcagacccgg caaaggaatt caacccggcc
      481 ccccctgccc tctcggaaac cgcgcaggag atgcggccgg ccgagatatt ctggatcgtc
      541 aagaacggac tcaagatgac cggcatgccc gaatcaggct ccacacattc ggacgacgag
      601 atatggggta tcgtggcgtt cgtcacgagg ctcccgggga tgacgccgga ggagtacaag
      661 cttatgaagc aaaacgcggc taaagaatcc ggccaccacc acgaacacga ctaaaaacga
      721 atagaagcgg aacggaagat catttataat tcgtgcatag aagaatatat agaggaggtt
      781 ttaaaatgaa ttcgggaatt tcaaggacca tttctttatt gattacggct cacgtactga
      841 ccgcggccgt cgtgatttat gcaggcgtgt cggcggctca gaactcggac agcagcccgg
      901 cgcagatgaa cgccgctcaa cctctcaagc aggtcgagtc caagtacgtg tgcatggtca
      961 cgaaccagct ctacgacaag gagcagatac ccgtagccgt aggggacaag acctactacg
     1021 gatgctgcca gatgtgcgag gcgaagctca aggaagaccc ttcgagccgc acggcgattg
     1081 accccgtaag cgggaaagag gtggataaat ctaccgccgt catcggcgcc tcggccgacg
     1141 ggaaggtcta ttatttcgag agcgtggaag acctgacggc gtacggtaag accgccaacc
     1201 aataaggcca tgcttcataa accgggggga cggggctgat tacgggaggg gacgtcaggc
     1261 gactacgatc ctgttgcggc cctgggattt agccttatag agcgccttat cggcggattc
     1321 gagaaggagc tcggccgacg tgccgtgctc ggaaaaaacg accacgccca tcgatacctc
     1381 taccgagctg atgaaggtcc cgcggtgcct gacacggacg cgcttgatct cctcgtggag
     1441 ctgctccgcc cttctgcatg tattttcgag agaagacccc gggagtatga ggacgaattc
     1501 ctccccgccg taacggcagg cgatgtcctc gcgcctgatg cggtccttga aaaagtcgcc
     1561 gatgctgcgc agcagcatgt ctcccgcggc gtggccgtag ttatcgttga aatgcttgaa
     1621 gtggtctata tcgaccataa tgagaccgag cggctccttg tttcgcgcga ccctgctgat
     1681 ttccctcttg agagtctcct ccatgtaacg cctgttgaag agcccggtga gggaatcgta
     1741 gatggcgtaa ttctggagcg cctgatggtg catgatgttg ccgagcgcga gcgccgtgag
     1801 cttggccatt gttgagacta tgtggctcgt atatgtgttt tcctcttcgg cttcaatccc
     1861 taccgcattc tcggcgttcc tctccaggta aagaagcccg agggtcttgc ccctcgcgag
     1921 catcgggacg cagtaatagt tcacgtcctt cgtctcgatg aggtgctgac agtacagctc
     1981 ggtcggagcc ttgttcacgt aatgcagcct gccgagcctg agagcccagc atttgtcggg
     2041 aaggaattcc cgctcgctgt tgatgttgtc cccccatgaa gtaacgcatt ccaggatgtt
     2101 aaggacgtcg ttgtatacga gcaaagcccc ggtctcgccc ggaaacagct tatggacgta
     2161 attcgttatt atcgtgtaga cctcttcctc gccgcgggaa gcctgtagca gctcgtccat
     2221 ctcgcatagc tgaatgagct tcctgttccg cttttcgagc tttgtaatga gggcctggat
     2281 gtctttcctt atagtcgagc tgttgtcgtc gtcattgagc tctatctcgc ggaatatttt
     2341 ctccataatg tccgccctgc cgcctgtgag tgtgtgtatg ttactgggct cgtccgtttc
     2401 cgcaatcccc ggatcaggct gtgcgccgcc cgagacgttc accggttcgg cagcgtgtcc
     2461 tccggagccc ggaacgccca gaccctgctc gagcctgagt atgacgttag ccgcgatggt
     2521 ctttaggagc gagacctgct cgaagctcag ttctttcggc tggaaaccgg aaatacacag
     2581 agcgcctatc ctgtggccgt tcctgtctat gagcggcagg cccgcaataa attttatatc
     2641 ctggtaggaa tggagaaaag ggttcgactt cgtcctctcg tcttcaaacg gataggacac
     2701 cgcgagcacg tcttcagggt ggtttatgac gtatgctccg aacgacttgt cgcgggggac
     2761 ctccctaagt ccggtctcga cgccgatgac cgacttgaac cactccctag tttcgtcaac
     2821 gaacgttata agggcgatcg ggctcttcga tatataggcg gcgagcctcg cgagctcgtc
     2881 gaacgcggga tcgggcgggg tatcgaggat gttataatcc agaagcgccc tgaaacgctc
     2941 gacttcctcg tcatgaagtc tcatgctcgt atttctaacg ttatctatat ctctattcat
     3001 atattaagaa ttggaccaga accccgacca gatattatat cacaaccggc ggggaaaata
     3061 ccattaacgg gttaaatata aaattatcaa ctgcaatgtc acgggaagat gaaagattcc
     3121 cgcaatcggc tataatatcg actaggaaaa gaaatcgagg gaatggctta tgagcgtatt
     3181 gaacacaggc acgaagatcg gggttctggt aatcatattc ctcgtgctgg cactccttgg
     3241 atatgcccta ttcggcgaaa gagagacggt caagacgtct cctctcgtgg ggaaggaagc
     3301 gccgggattc accttgaagc tgttcgacgg gagaagggta agcctcgagg agctaagggg
     3361 gaaaaccgtg ctcctcaatt tctgggcgtc ctggtgcatg ccgtgcaggc aggagtctca
     3421 ggcgctcgag gagtcctggc tcaaatataa ggataagaac gtggtattca tcggcgtgaa
     3481 cgtgtgggac gagaacgaaa gcgccgcttc ttatatagag aagttcggag gcggataccc
     3541 gcaggggatc gaccctgagg aggagataca ggtcaattac gggatagggg gcgtgcccga
     3601 gacctatttc ataagccccg ccggtattat tacggataaa tacgacggcc ctctgacgga
     3661 agaaataatt gactattacg ttatgaaggc gggcggcgcc gagagggaaa cgtcgtcgat
     3721 gaattaggat ttacgttatg accatgaaaa gctttcaaaa attaaatcct gcggcattaa
     3781 ccctgacgct tttgctgatc ctgtttttcc gggcgggcat agcttccccg gccgagagcc
     3841 tggacgaccg cgtgaacgag atcgcccatc tgctcatgtg cccggtgtgc caggggcaga
     3901 gcgtcgcgga atcgaactcg aacctcgccc aggatatgag gcagataatc aggaagcagc
     3961 tcgaagaagg aaagtcgaaa gaggaagtga tcgcgtactt cgtcaaccgc tacggggaga
     4021 cgatactggc gtcgcctccg cccaagggcg tgaactggct cctctggttg ctgcctgcgc
     4081 ttgcgatatt gttcgggggg ttgggcatag gtatctacct tcataaaacc gggaagggaa
     4141 aacggagcgc ctcagctaag accgaaaagc ccgcggccga cagcgattac atgctgaagc
     4201 tcgacgagga gctgaagaag cacgagtcgt gaggggattc acgatgaagg ttgtcgagaa
     4261 tgacagaaag gaaaaagaaa aatcctccct aaccctcctt tttctaagga gagaattaaa
     4321 gatcgaaaga caagattgcc gcggctcctc tggagcctcg caattgtaga tgttaaataa
     4381 ttcggggctg gaattctcct gaatggcacg gaatgcaggg ggatttatct tttccgacat
     4441 tacagaattt gcgttaagaa aatcgcgtgt ttaagcgtaa aaaatctttc agcttcccat
     4501 tgaaagattt tagattaaac gcaagatttt tagcaaatta tgtagtcgga tgatttttgc
     4561 ttcttttcat caagggaaag aagaaaaaca tattttgcct tctttacgta ttccaagccc
     4621 ttaagaaaac tcagggctaa caacgctcag ggactttatc agtttataat ctcgttcctt
     4681 ttgtccccga attacttgac acctacagca atgacaggac gaataatacg tccttcgaca
     4741 agctcaggac gaacggggcg gtgagtcacc gcagatgtat gggactggcc tcgcgtaatt
     4801 aacccccgaa aaatgcgcgg gcgtcgtcga gaactttttc ataatctccc gggatgtcga
     4861 accattttat ctccttgtcc ctccttaacc acgtcatctg ccgcttggca aaacgttttg
     4921 tgtcgcgctt gataagctcg accgctttct caagactgat ctcgccgtcg atgtacctgt
     4981 ttatctcttt ataacctatg gactgcatcg gtttcaattc gggaccgtac ccaattctcc
     5041 tcagtcccgt gacttcctca acgaggccct cttccatcat cctgtcgacc ctcaga
//
LOCUS       DASSJH010000400         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1502965, whole genome shotgun sequence.
ACCESSION   DASSJH010000400 DASSJH010000000
VERSION     DASSJH010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502965"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..315
                     /locus_tag="VFJ67_08825"
     CDS             37..315
                     /locus_tag="VFJ67_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HET7290525.1"
                     /translation="MEDKKRVEITYCRQCRWLLRAAWMAQELLTTFDLEIEEMALVPG
                     TGGIFEVRVGDELVWSRKEKGRFPDIKELKQFVRDRVAPGRDLGHSDV"
     gene            419..1126
                     /gene="aat"
                     /locus_tag="VFJ67_08830"
     CDS             419..1126
                     /gene="aat"
                     /locus_tag="VFJ67_08830"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET7290526.1"
                     /translation="MPVYLLDDSLAFPHPMNAEPSGLLAIGGDLSLPRLLLAYRSGIF
                     PWYSENNPILWFSPDPRLVLFLSDLYVSKKLRKILNSGQFEVRFDTSFGEVIRSCSAV
                     SREGQTGTWITGDMIDAYLSLHEAGYAHSVETYQEGKLVGGLYGVAIGGVFFGESMFH
                     LLSDASKVALYYLVERLRVWGFDFIDSQVPNSHMKRMGGKEVPRERFLAMLGQSVNRK
                     TVAGKWNVERDLKSGRL"
     gene            1188..1823
                     /locus_tag="VFJ67_08835"
     CDS             1188..1823
                     /locus_tag="VFJ67_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7290527.1"
                     /translation="MKSVLCYGDSITWGFNPADGSRFSFGDRWPGILQTELGPGYRVI
                     EESVPGRTTNWDSPYLPDRNGRKSLPLVLESHMPIDLFILLLGTNDLWKALALSASDI
                     AASAMSLIWTVQKAMAGPGFGVPEILLIAPQPLGPLSPFMEFYFEGRAPVSRGLAKEY
                     EKVSRMTGCHFLDASKFVKSSKIDGVHLNAPGQRKLALGIKKSVLSILGGK"
     gene            1965..2420
                     /locus_tag="VFJ67_08840"
     CDS             1965..2420
                     /locus_tag="VFJ67_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HET7290528.1"
                     /translation="MSDWKVINHGHPDIKPSGDRRAIFIGRYQPYHMGHISLVRQKLD
                     KGIPCLIMVRDIPPDPQNPFTTEQTVDMIRKYHASKGDDVVVMIIPDIESVNWGRGVG
                     YEMNEFFPPEDIGFISATKIRNAITEGNDDWRKLVDESIQEDVVKYLKG"
     gene            2630..2953
                     /locus_tag="VFJ67_08845"
     CDS             2630..2953
                     /locus_tag="VFJ67_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007420063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HET7290529.1"
                     /translation="MVNPDIGEREFSSFRQFYQYYLSEHTSPLNRRLHFTGCIIVLII
                     VLTAFLARNLSLILLAPVFGYGLAWSGHYFVEKNKPATFKHPIWSLMSDWMMFKDIMT
                     GKIKL"
     gene            3096..3842
                     /locus_tag="VFJ67_08850"
     CDS             3096..3842
                     /locus_tag="VFJ67_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7290530.1"
                     /translation="MPRLLVIQHVAHEILGTLDPLLRNSGFRIKYVNFERHPDLKPGL
                     GGYDGLIVLGGPMNVDEVDMYPNLSREVDSIRSAIDSGMPVLGICLGSQLIAKALGAK
                     VYKNKEKEIGWYDLSPNEAGRRDPLLSHFSGTEKIFQWHGDTFDIPEGAVLLASSPLC
                     RNQAFRYGDNVYGLQFHLEVDEPMVERWLRIPGNRKEIEELDGKIDPDRIRKETHEYI
                     SRLKELSDKSFAGFIGFFGFNKKRQSLPSR"
     gene            complement(3850..>4645)
                     /locus_tag="VFJ67_08855"
     CDS             complement(3850..>4645)
                     /locus_tag="VFJ67_08855"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET7290531.1"
                     /translation="EENIQEMIAKGTLLVDTEGMVTGQINGLAVYNMGDYAFGKPSRI
                     TCETFMGTEGVVNIERKSRMSGNIHDKGVLILSGYIGARYAQNKPLSLSASIGFEQSY
                     ETIDGDSASAAELIVLLSSLSGVPVKQGLAITGSVNQKGQIQPIGGVNEKVEGFYDVC
                     KAKGMTGEQGVAIPYQNVKNLMLKKEVVEAVKRGRFHIYPVETIDQAIEVMTGKEAGT
                     RGASGKFRRGSINYLVDKRLREYAEDYRKFGKQQQAHKRTDNSNEK"
BASE COUNT         1040 a         1297 c         1262 g         1046 t
ORIGIN      
        1 tgataaaata ccggtgacac gggactcgcg acgacgatgg aagataagaa gagggtcgaa
       61 ataacctatt gcaggcaatg ccgctggctt ctgagggccg cctggatggc tcaggagctg
      121 ctcacgacat ttgatctgga aatagaggaa atggctctcg tgccgggcac gggcggcata
      181 ttcgaggtca gggtcgggga cgagctcgta tggtcgagga aggagaaggg caggttcccc
      241 gatatcaagg agctgaaaca gttcgtgagg gacagggtcg ctcccggcag ggacctcgga
      301 cactcggacg tataacgccg catcggttaa agaggctcaa tcggcgaagg ccgcgtacag
      361 ccgggattaa ggcttccggg aagaacgcac cgccggcggc tgtgtataat atttctcaat
      421 gcccgtatac cttctcgacg actcgctcgc cttcccccat cccatgaacg cggagccaag
      481 cgggctcctc gcgataggag gagacctctc cctgccgaga ctcctgcttg cgtacaggag
      541 cggcatattc ccctggtact cggaaaacaa tcccatactg tggttttctc ccgacccgag
      601 gctggtgctt tttctctccg atctctacgt ttcgaagaaa ttgaggaaaa tactgaattc
      661 gggacagttc gaggtccggt tcgacacgtc gttcggcgaa gtcataagga gctgctcagc
      721 ggtgagcagg gaagggcaga ccggcacatg gatcaccggg gacatgatag acgcctacct
      781 ctcactccac gaagcgggat acgcccactc ggtcgaaacc taccaagagg ggaagctcgt
      841 gggcgggctc tacggtgtcg caattggagg cgtttttttc ggcgaatcca tgtttcacct
      901 tttgagcgac gcttccaagg tcgcgcttta ctaccttgtg gagaggctca gggtgtgggg
      961 cttcgatttc atagattcgc aggtgccgaa tagccatatg aagaggatgg gagggaagga
     1021 agtcccgagg gagcgtttcc tggcaatgct cggccaatcg gtgaacagga agacggtcgc
     1081 cgggaaatgg aacgtcgaac gggacttgaa atcgggaagg ctctgagtaa gactgtcgtg
     1141 aataattccg cttatattga ataaatcctg cgcttgggag gaaatccatg aaatcagtcc
     1201 tgtgttacgg agattccatt acttggggct ttaatccggc ggacgggagc aggttttcgt
     1261 ttggagaccg ctggcccgga attctccaga ccgagctcgg cccgggctac agggtgatag
     1321 aggaatcggt gccgggcagg acgaccaact gggacagccc ctatctcccg gacaggaacg
     1381 gcaggaagtc cctcccgctc gtgctcgaga gccatatgcc gatcgatctt tttatacttt
     1441 tgctcggtac gaacgacctc tggaaagcat tggccctttc cgcatccgac atcgccgcgt
     1501 ccgctatgtc tctcatatgg acggtgcaga aggccatggc ggggccgggt ttcggcgtgc
     1561 ccgaaattct tctgatcgcc ccgcagccgt taggcccact ctctcccttt atggaattct
     1621 atttcgaagg ccgcgctccg gtttcgagag ggctggctaa agaatacgaa aaggtctccc
     1681 ggatgaccgg ctgtcatttt ctcgacgcct cgaaattcgt gaaatcgagc aaaatcgacg
     1741 gcgtccacct caatgccccc gggcagagga agctcgctct gggcataaaa aagtccgttc
     1801 tctcgatact gggcggaaag tgagctatgc tgcggcggat gacactaaaa ttgaaaattg
     1861 acggacggca tgtataatga agtccgtttt aatatcgaag agccggcctg aataataaaa
     1921 tccagaaaga acagcgaacg tatctatacg caaggaggct ttaaatgagc gattggaagg
     1981 tcataaacca cggacacccg gatataaaac cgtccgggga caggagggcc atttttatag
     2041 gcaggtatca gccctatcac atgggtcata taagccttgt caggcagaag ctcgacaagg
     2101 ggataccctg cctgatcatg gtgagggaca tcccgcccga cccgcagaac ccgttcacga
     2161 ccgagcagac cgtggacatg ataagaaagt accacgcgag caagggggat gacgtcgtgg
     2221 tcatgatcat cccggatata gaatcagtca actggggaag gggagtgggc tacgagatga
     2281 acgagttctt ccctcccgaa gatatcgggt tcatctcggc gacgaaaata aggaacgcca
     2341 tcaccgaggg caacgacgac tggcgcaagc tcgttgacga gtccatccag gaggacgtgg
     2401 tcaaatacct caaaggttaa gtgcctcttc agaaccagcc ggaatcattc aaataatcat
     2461 cgatattgca tagagggagg gacggccgcc gggcccttcc tccctctttt tatcgagggg
     2521 cctgattctc tcttcccctt cgcccgacat attgattttc tctcccccgc acgtgttttt
     2581 aaaaacgagt ttataattgt aataatcaac aaaaaaccgg gcgcgagaaa tggtcaatcc
     2641 tgatattgga gaaagagaat tcagcagctt tcgtcagttt tatcaatatt atctgtcaga
     2701 acacaccagc cctctgaaca gacggctgca tttcacgggg tgtattatcg tgcttataat
     2761 agtcctgacc gcgtttctcg cccgtaacct gtcgctcatc ctcctggccc ccgttttcgg
     2821 atacggactc gcctggtccg gacactattt cgtggagaag aacaagcccg ccacattcaa
     2881 gcacccaatc tggagcctca tgagcgactg gatgatgttc aaggacatca tgacggggaa
     2941 gatcaagctc taaggcattc cctcttcgat aattgcgctc catcgagtcc gcccgtttcg
     3001 ctattcgcag gttatcgttt acgttcccgc ctattaacgg gtaaaatcct ctttaccttt
     3061 tccggaatcc tcagccggga aatggcctga acccaatgcc cagacttctg gtaatccagc
     3121 acgtagctca cgagatactc ggtacgctcg accccctgtt aaggaactcc gggttcagga
     3181 taaagtacgt caatttcgag cggcacccgg acctcaagcc gggcctcgga ggctatgacg
     3241 gcctaatcgt gctcggaggg ccgatgaatg tggacgaggt ggacatgtac cccaatctct
     3301 cccgcgaggt tgattcgatc cggagcgcca tcgattccgg catgccggtg ctcggtatat
     3361 gcctcgggtc tcagcttatc gcgaaagcgc tcggcgcgaa ggtctataag aacaaagaga
     3421 aagagatcgg ctggtacgac ctctccccga acgaggcggg caggagggac cctctcctct
     3481 cccatttctc gggcacggaa aagatattcc agtggcacgg ggacacgttc gacatccccg
     3541 aaggggcggt gctgctcgcg tcgtcacctc tctgcaggaa ccaggcgttc cgctacggcg
     3601 ataacgtcta cggcctccag ttccacctcg aggtggacga gccgatggtc gagaggtggc
     3661 tccgcatacc cggcaacagg aaagagattg aggagcttga cggaaagata gaccccgaca
     3721 ggattagaaa ggaaacccac gaatacatat cccgcctcaa ggagctgagc gacaaatcgt
     3781 tcgcgggctt catagggttc ttcggtttca ataaaaagcg gcagtctctc ccgtcgaggt
     3841 aacagtctcc tacttctcgt tcgagttgtc cgtcctcttg tgcgcctgct gctgtttccc
     3901 gaacttcctg tagtcctcgg cgtattccct aagcctcttg tcgacgaggt aatttatgct
     3961 ccccctcctg aatttcccgc tagcgcccct cgtgccggct tctttccccg tcatcacctc
     4021 tatagcctgg tctatggtct cgaccgggta gatgtggaat ctccctctct ttaccgcctc
     4081 caccacctct tttttgagca taaggttctt cacgttctgg tacggtatgg ccactccctg
     4141 ctcgcctgtc attcctttag ccttgcagac gtcgtagaac ccttcgacct tttcgttcac
     4201 cccgccgatg ggctgtatct gtcccttctg gtttaccgaa cccgtgatcg cgaggccctg
     4261 ctttacgggg actcccgaga ggctcgacag cagcacgata agctccgccg cggacgcgct
     4321 gtccccgtct atcgtctcgt agctctgctc gaaccctatg ctcgccgaga ggctgagagg
     4381 cttgttctgt gcgtatctgg ctccgatgta cccgctgagt ataaggacgc ccttgtcgtg
     4441 tatgtttccg ctcatccgcg atttcctttc gatattcact accccttccg ttcccatgaa
     4501 cgtctcgcac gttatccggg acggcttccc gaacgcgtag tcccccatgt tgtacacggc
     4561 gagcccgtta atctgccccg tgaccatgcc ttcggtatcg acgagcagcg tccccttcgc
     4621 tatcatctcc tggatgttct cctcg
//
LOCUS       DASSJH010000401         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_95934, whole genome shotgun sequence.
ACCESSION   DASSJH010000401 DASSJH010000000
VERSION     DASSJH010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95934"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VFJ67_08860"
     CDS             complement(<1..1137)
                     /locus_tag="VFJ67_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET7290532.1"
                     /translation="MKSIQEIIGERNPAALLKIDELLSKNGESLEEILIKSRVLDEQE
                     VLEVLSEFYEFPYTLTISEREIDTELVKTLPISFAKKFRLMPFQRKNGKVMIILSPPI
                     DLYALDEIRSLFGCEIEPVLALNHTVIDTINRVYERGKEMTEGIEDDSAGISEYDLQE
                     PKDLLEAEDEAPIIRFVNSLLFQAVKEKASDIHLECFEKELSVRFRKDGILHEITAVP
                     KKLQASIISRVKIMAELDIAEKRKPQDGRIRVKVAGRDVDVRISTVPTTWGESVVMRI
                     LDRSSVILSLEDLGLAGKKLQVFEALIQRPHGIILVTGPTGSGKTTSLYAGLERINSP
                     DKKIITVEDPVEYQIQGINQIQVNPKVNLTFANGLRSILRQDPDV"
     gene            complement(1154..2521)
                     /locus_tag="VFJ67_08865"
     CDS             complement(1154..2521)
                     /locus_tag="VFJ67_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HET7290533.1"
                     /translation="MGFKVITDERTNSLIVIAYPDDMKKIKSVIEILDVETAEPEEGI
                     YVIRVLNADAEQLVSVLASLFGGGGSTGIGANRAQRNQGSPFGTDSALNSSSSTFGSG
                     SSASRQAIDRGVTSGSAVVAETDELRITSDPSTNSIIFIGSRRDYETVKEVIQELDVR
                     RKQVFVEAAILEVSLDKIRSFGTNLSIGFTVNGDTLGFGGTVLPGVPSLLGVAADPEA
                     SVNVLGSLSGLFLGVIGEEVDPDGSGPIPPIPSFSALFQALTSLTDVNVLSTPSIITT
                     DNEPAEIIVADVIPFPTGSTVGDSGVTVQTIQREPVGIRLSILPQISEGDFLNLNILT
                     EVSAIRQAPVQGLNTAQFGIATTTRTADSSVVVKNGQTIVIGGLVQDRESVLQNRVPV
                     LGNIPVIGNLFKFKQRESQKINLMILLTPRIVENEQDMQRILEERQRRNMLLQERGFN
                     REDNN"
BASE COUNT          493 a          712 c          703 g          708 t
ORIGIN      
        1 tacgtctggg tcctgtctga gaatagacct tagaccgttt gcgaatgtga gatttacctt
       61 cggattgacc tgtatctggt ttatcccctg aatctgatat tcgacggggt cttccacggt
      121 aatgatcttt ttgtctgggg agtttattct ttcgagcccc gcgtagagcg acgtcgtttt
      181 accgctgccc gtaggacccg tgaccaatat gatgccgtga gggcgctgta tcagcgcctc
      241 gaatacctgg agcttctttc ccgcaagacc gaggtcctcg agggagagga tcacggatga
      301 tctgtcgagg atcctcataa cgacgctctc tccccaggtt gtcggtactg tcgatatacg
      361 gacgtccacg tccctgcccg cgaccttgac tcggattctt ccgtcctggg gttttctctt
      421 ctccgctatg tcgagctcgg ccatgatctt gaccctcgag ataatggagg cctgaagctt
      481 tttgggcacg gccgtgattt cgtgaagtat gccgtccttt ctgaacctta ccgaaagctc
      541 cttctcgaaa cactctaggt gaatatcgct cgctttttcc ttgacggcct gaaagaggag
      601 tgaattgacg aatcttatta tcggggcttc gtcttcggcc tcgaggaggt ctttaggctc
      661 ctggaggtcg tactccgaga tgccggccga gtcgtcttcg atcccttcgg tcatctcctt
      721 cccgcgctca tacactcggt taatggtatc gatcaccgtg tggttgagcg caaggacagg
      781 ctctatctcg catccgaata gcgatctgat ttcatcgagc gcgtagagat cgatgggcgg
      841 ggacagtatt atcattacct ttccgttctt cctctggaaa ggcatgagcc tgaatttttt
      901 cgcaaaggag atagggagcg ttttcacgag ctccgtgtct atctcccttt cggatatagt
      961 cagtgtgtag gggaattcgt agaattcgga aagcacctcg aggacttcct gctcgtcgag
     1021 gactctcgac ttgatgagaa tctcttcgag gctctctccg ttcttcgata acaactcgtc
     1081 tattttgagg agcgctgcgg gatttcgctc gcctattatt tcctggatgc tcttcatggc
     1141 aataacttaa gcttcagtta ttatcctccc tgttaaaccc cctctcctgt aaaagcatat
     1201 tgcgtctttg tctttcttca agtatgcgct gcatatcctg ctcgttctcg acgatcctgg
     1261 gagtaagcag tatcatgagg ttgatcttct ggctctccct ctgtttgaat ttgaacaggt
     1321 tgcctataac ggggatgttc ccgagcaccg ggaccctgtt ctgaaggacg ctttccctgt
     1381 cctgtacgag gccgccgatc acgatcgtct gcccgttctt gacgacgacg gaggaatccg
     1441 ccgtcctcgt cgttgtggcg ataccaaatt gcgcagtatt cagaccctga acgggcgcct
     1501 gcctgatcgc ggaaacttcc gtgaggatgt taaggttgag gaagtctcct tcgcttatct
     1561 gcggtagtat ggaaagcctg ataccgacgg gttcgcgttg tatcgtctgt acggtgaccc
     1621 cgctgtctcc cacggtgctg ccggtcggga acggaataac gtcggccacg atgatctcag
     1681 cgggctcgtt atcggtcgta atgatgctcg gcgtcgagag cacattcacg tctgtcagcg
     1741 atgtcaacgc ctggaataga gcgctgaagg aaggtatcgg cggtattgga ccggagccgt
     1801 cggggtccac ttcctcgcca atcacgccga ggaacaagcc ggaaaggctg cccagcacgt
     1861 tcacggacgc ttccggatcg gctgcgacac cgagtaggct gggaactccg ggaaggaccg
     1921 ttccgccgaa tccgagcgtg tccccgttga cggtgaagcc tatgctgagg tttgtgccga
     1981 acgatctgat tttatccagg ctgacttcga gaatcgccgc ctccacgaat acctgtttcc
     2041 gcctcacatc cagttcctgt attacttcct ttactgtctc atagtccctt ctggaaccta
     2101 tgaatattat ggagtttgtc gacgggtcag atgtaatccg gagctcgtcc gtctccgcta
     2161 ccactgcgga gccgctagtc actcccctat cgattgcctg cctcgaggcg gaggagcccg
     2221 aaccgaatgt ggacgatgaa gagttgagag ccgagtccgt accgaaaggc gagccctggt
     2281 ttctctgcgc cctgtttgct ccgattccgg ttgagccgcc gcctccgaac aggcttgcga
     2341 gcacggacac cagctgctcg gcgtcggcgt ttaatacgcg tatgacgtat atgccctctt
     2401 cgggctcggc agtttctacg tcgagtatct ctattacgga tttaatcttt ttcatgtcgt
     2461 ccggataagc tatcactatc agggagttgg tcctctcgtc cgttattacc ttgaaaccca
     2521 ccgcggaatt ttctccggtg gtttgggacg tgatagcggg agcttccgac ctgttttgtg
     2581 acccggggag gttacgggtt ctcgcagcgg acgtcg
//
LOCUS       DASSJH010000402         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_205006, whole genome shotgun sequence.
ACCESSION   DASSJH010000402 DASSJH010000000
VERSION     DASSJH010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205006"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2293)
                     /gene="metH"
                     /locus_tag="VFJ67_08870"
     CDS             complement(<1..>2293)
                     /gene="metH"
                     /locus_tag="VFJ67_08870"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016757870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET7290534.1"
                     /translation="VASEPDISRVPVMIDSSKFPVIEAGLKCIQGKGIVNSISLKEGE
                     EKFIEQARKVMRYGAAAVVMAFDEEGQAATKDDKVRICRRAYEILTEEVGMDPEDIIF
                     DPNVLTVATGIEEHNGYAIDFIEAVREIKKACPGAKTSGGISNVSFSFRGNNVVREAM
                     HSAFLYHAINAGLDMAIVNAGMLEVYEEIDKDLLQKVEDVLLNRRPDATEILVEYAEK
                     IKDTGKTKEKEAETWREGTVEERLSHALVKGIDTYIEKDTEEARLKHEKPLDVIEGPL
                     MDGMKIVGELFGEGKMFLPQVVKSARVMKKAVAWLEPFMEEEKKKAGSSGKVRGKFVI
                     ATVKGDVHDIGKNIVSVVLGCNNYEVFDLGVMVPCENILKKAKEVEANIIGLSGLITP
                     SLDEMTHNASEMERLGFRTPLLIGGATTSKAHTAIKIAPHYSGVVSHVQDASLVVGVC
                     NDLLNPEKYEEFVREQEKKHGELRKLHEKSKTATGFLTIEKARAKGFRDDWKKALIDA
                     PEKLGVQVFDEISLEYVIPFIDWSPFFWVWELKGQYPKILEHKKWGRQAKNLYDDAQK
                     LLRRVVSEKRFKPRAVLGFWPASAIGDDVEVYADKGRKEKLAELHFLRQQKDRGEKEK
                     YLSLSDFIAPAESGREDYIGGFVVTAGEEVETYAEYFKDKHDDYSAIMVQALGDRIAE
                     AMAEMMHKKARENWGYGRSENLSHEDLIKEKYRGIRPAPGYPSCPDHTEKEILWRLLD
                     AGKNIGVRLTENYAIYPPSSVAGL"
BASE COUNT          365 a          728 c          560 g          640 t
ORIGIN      
        1 gagtcccgcg acggagctcg gggggtaaat ggcgtagttc tcggtaagcc tcacccctat
       61 gttcttcccg gcgtcgagga gcctccagag tatctccttt tccgtgtggt cgggacatga
      121 aggatatccg ggggcgggcc ttatgcccct gtatttttcc tttataaggt cctcgtgaga
      181 aaggttctcg ctccttccgt agccccagtt ctcccgcgcc ttcttgtgca tcatctcggc
      241 catcgcctcg gcgatcctgt ctccgagggc ctgcaccatt atcgccgaat agtcgtcgtg
      301 tttatccttg aaatactcgg cgtaagtttc gacttcctcg cccgccgtca cgacgaagcc
      361 gccgatatag tcttcgcgcc cggattccgc gggcgctatg aaatccgaga gggagaggta
      421 tttctccttc tctcccctgt ccttctgctg gcggaggaag tggagctcgg ccagtttctc
      481 ctttcttccc ttgtctgcgt aaacctcgac gtcgtcccct atcgcggacg cgggccagaa
      541 cccgagcacc gctctcggtt tgaacctctt ttcggatact acgcgtctca ggagcttctg
      601 ggcgtcgtcg tagaggttct ttgcctgtct tccccatttc ttgtgctcga gaatcttcgg
      661 gtattggcct ttcagctccc atacccagaa gaagggcgac cagtctatga acggtatcac
      721 gtattcgagg cttatttcgt cgaatacctg aacgcccagc ttctcgggcg cgtcgatcag
      781 ggctttcttc cagtcgtccc tgaacccttt ggccctagcc ttttctatcg tgaggaaccc
      841 ggtcgcggtc ttcgatttct cgtgaagctt gcgcagctcg ccgtgcttct tctcctgctc
      901 tctcacgaat tcttcgtatt tctccgggtt cagcaggtcg ttgcacacgc ccactacgag
      961 agaggcgtcc tgcacgtggc tcacgacgcc gctgtagtga ggggctattt ttatcgccgt
     1021 gtgggccttg ctcgtcgtcg cccctcctat gaggagcggc gtcctgaacc cgagcctctc
     1081 catctcggag gcgttgtggg tcatctcgtc gagtgagggc gtaatgagac cgctcaaccc
     1141 tatgatgttc gcttcaactt cctttgcctt cttgagtatg ttctcgcacg ggaccataac
     1201 ccccagatcg aacacttcgt agttattgca gccgaggacg accgagacta tgttctttcc
     1261 tatgtcgtgg acgtctccct tgacggtagc gatgacgaat ttccccctca cctttccgct
     1321 gctcccggct ttcttcttct cttcctccat gaagggctct aaccaggcga ccgccttttt
     1381 catcacgcgc gcgctcttta cgacctgagg caggaacatc ttcccttcgc cgaagagctc
     1441 acccacgatc ttcataccgt ccatgagcgg gccttctatt acgtcgaggg gcttttcgtg
     1501 tttgagtctc gcctcttccg tgtctttctc gatataagtg tctattccct tcacgagcgc
     1561 gtgggagagc ctctcctcga ccgtcccctc cctccacgtc tcggcctctt tttccttcgt
     1621 cttgcccgtg tcttttatct tctcggcgta ttcgacgagt atctctgtcg cgtctggtct
     1681 cctgttgagg agcacatcct cgaccttttg cagaaggtct ttatctattt cctcgtacac
     1741 ctcgagcatc ccggcgttca ctatggccat gtcgagcccg gcgtttatcg cgtgatagag
     1801 gaacgcgctg tgcatcgcct ccctcactac gttgtttccg cgaaacgaga acgaaacgtt
     1861 gcttatgccg ccgctcgttt tggctcccgg gcatgctttc tttatctcac gcacggcctc
     1921 tatgaaatca atggcgtatc cgttgtgctc ctctatgccg gtcgccactg taagaacgtt
     1981 gggatcgaaa atgatatcct cgggatccat gccgacttcc tctgtcagaa tctcgtacgc
     2041 cctcctgcaa atcctgacct tgtcatcttt agtcgccgcc tgtccctcct cgtcaaaggc
     2101 catcacgacg gcggctgcgc cgtagcgcat aaccttccgc gcctgctcta tgaacttttc
     2161 ctccccttcc ttgaggctta tggagttaac aatgcccttc ccctggatgc acttgaggcc
     2221 cgcttctatc accgggaact tcgagctgtc tatcatcacg gggactcgcg atatgtcggg
     2281 ctctgaagcc acg
//
LOCUS       DASSJH010000403         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_205341, whole genome shotgun sequence.
ACCESSION   DASSJH010000403 DASSJH010000000
VERSION     DASSJH010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205341"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFJ67_08875"
     CDS             <1..804
                     /locus_tag="VFJ67_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7290535.1"
                     /translation="SGGKRFMHGYSELGELAPRDIVSRAIYLEMKKSGTEYVHLDLRH
                     LDPSYVKTRFANIHQACLAHGIDMTTSLVPVAPAAHYTIGGVRTALSGQTNIDGLFAC
                     GEVACTGVHGANRLASNSLLECVVFAKRAVDGANESSMSGNPVTGSGIDLDLLNISWS
                     DEGLFKELRREASRIMNRHVGMVRNREGLEAALAELKTMTDSLDKLSGYYRLKLKMIL
                     EVSAIIARFSLLREETRGVHIREDFPGEDPGWRKHIILKKGEEPAVLPV"
     gene            854..1699
                     /gene="nadC"
                     /locus_tag="VFJ67_08880"
     CDS             854..1699
                     /gene="nadC"
                     /locus_tag="VFJ67_08880"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET7290536.1"
                     /translation="MKRIDLDFNRVDRIIEYALREDLGDGDVTTNSLATEKEDSKAVI
                     RAKAKGVIAGLPIAKRVFQKLDPTVVCVDNISDGENIKPGDILSEINGGTRAILSGER
                     LALNILQRLSGIATLTSKYVKAVEGLPVKILDTRKTAPGLRILDKYAVSVGGGFNHRF
                     GLFDGVLIKDNHIAIAGGISEAVAVIRKRYKQKYKIEIETTDMDQVKEALIAGADIIM
                     LDNMNPEQMRKAVRLIDRNALVEASGGITLRNVRDVAETGVNFISVGALTHSAPALDI
                     GLYMV"
     gene            1736..>2608
                     /gene="nadA"
                     /locus_tag="VFJ67_08885"
     CDS             1736..>2608
                     /gene="nadA"
                     /locus_tag="VFJ67_08885"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET7290537.1"
                     /translation="MEDYSPISEEIKRLKEEKNAVILAHNYQLPEVQDVADFTADSLA
                     LSQMAARTEAEIIVFCGVHFMAETASIISPRKKVLLPDLGAGCSLADTITADQLREWK
                     KEHPGAVVVSYVNTTAEVKAESDYCCTSSNAVKVVNAVPEGKEILFLPDMFLGAFAAK
                     ATGRKIHIWPGECHVHAGIKTDDLKEMKHSHPGAELVVHPECGCTTNLLYQGFNGGAK
                     NGNGHIKFLSTGGMTKFVKESQAREFIIATETGMLYKLRKDNPDKTFLPARENAVCKY
                     MKMITLEKVLRSLED"
BASE COUNT          673 a          660 c          776 g          499 t
ORIGIN      
        1 agcgggggaa agaggttcat gcacggttac agcgagctcg gcgagcttgc tccgagggac
       61 atcgtctccc gcgcgatata cctggaaatg aaaaagtcgg ggaccgaata cgttcacctt
      121 gacctgaggc acctcgatcc gagctatgtc aaaacccggt tcgcgaatat ccaccaggca
      181 tgcctcgccc acgggataga catgacgacg agcctcgtcc ctgtagcgcc cgcggcacac
      241 tatactatcg ggggagtgag gacggccctc tcagggcaga cgaacatcga cggcctcttc
      301 gcctgcggcg aggtcgcgtg cacgggagtg cacggggcga acagactcgc gagcaactct
      361 cttcttgaat gcgtggtatt cgccaagcgc gccgtcgacg gcgcgaacga atcttcaatg
      421 agcggaaacc ctgttacggg ttccggcatc gacctggacc tccttaatat ctcgtggtcg
      481 gatgagggat tattcaaaga gctcaggagg gaagcttcgc gtataatgaa ccggcatgtc
      541 gggatggtaa ggaacaggga gggccttgaa gcagcgctgg cggagcttaa aacgatgacg
      601 gactccctag acaaactctc gggctactac aggcttaaat tgaagatgat cctcgaggtt
      661 tcggctatca tcgcccgctt ctcgcttctg agagaggaaa cgaggggggt gcatatcaga
      721 gaggactttc ccggagagga ccccgggtgg aggaagcaca ttattctgaa aaagggagag
      781 gagcctgccg ttctgcctgt atgaaaggta tattacttac cggagcgggg agatacgcat
      841 tacggcttga cgcatgaaaa gaatagatct cgatttcaac cgcgtggacc gcatcataga
      901 atacgccctg agggaagacc ttggtgacgg cgacgttacc accaactcgc ttgcgaccga
      961 gaaggaggac agcaaggctg taatccgcgc caaggcgaaa ggggtgatag ccggcctccc
     1021 gatcgcgaag cgcgtgtttc agaagctcga tccgaccgtg gtgtgcgtcg acaatataag
     1081 cgacggcgaa aacatcaagc cgggcgacat actgtcggag attaacgggg gcacgagggc
     1141 gattctctca ggcgaacgcc tcgccctgaa catccttcaa agactatccg gaatagccac
     1201 gctcacgtct aaatacgtaa aggcagtgga gggtctgccg gtaaagattc tcgatacgcg
     1261 aaaaacggct cccggactcc gcatactcga caaatacgcg gtctcggtag ggggcggatt
     1321 caaccaccgc ttcggactct tcgacggtgt cctaataaag gacaatcaca tcgcaatagc
     1381 gggaggtata tctgaggcgg tagcagtcat tcgcaagaga tacaagcaga aatacaagat
     1441 agagatcgag accaccgaca tggatcaagt aaaggaagcg ctcatagcgg gggcggatat
     1501 aataatgctc gacaacatga acccggagca gatgaggaag gcggtgcgtc ttatagacag
     1561 gaacgcgctc gtcgaggcct cgggcgggat cacactcagg aacgtaaggg atgtggccga
     1621 aacgggcgtg aattttatat ccgtgggcgc cctcactcat tcggccccgg ctttagatat
     1681 agggctctat atggtataat aattttacca atgatgtaag cgggttgagg ggcacatgga
     1741 agactattcc ccgatatcgg aagagataaa gaggcttaag gaagagaaga acgccgttat
     1801 actggcgcat aactatcagc tgccggaggt tcaggacgta gcggatttta cggccgattc
     1861 gcttgcgctt tcgcagatgg ccgccagaac ggaagccgaa atcatagtat tctgcggcgt
     1921 gcatttcatg gctgaaacgg cctcaatcat ctcgccccgg aagaaggtgc tactgccgga
     1981 cttgggtgcg ggctgctccc ttgccgatac gataactgcc gaccagctcc gggaatggaa
     2041 gaaggagcat cccggcgcgg tggtggtctc ttacgttaat acgacagccg aggtgaaggc
     2101 cgagagcgat tactgctgca cttcttcaaa cgcggtcaag gtagtgaacg ctgtgcctga
     2161 aggcaaggag atacttttcc tcccggatat gtttctcggg gcgttcgcag cgaaagcaac
     2221 gggcaggaaa atccatatct ggcccggcga gtgccacgtt cacgcgggaa taaagacgga
     2281 cgaccttaag gaaatgaagc actcgcatcc gggcgcggag ctcgtcgtcc atcccgaatg
     2341 cggatgcacc actaatctcc tctatcaggg tttcaacggc ggggctaaga acgggaacgg
     2401 ccatattaaa ttcttatcga ccggcgggat gaccaaattc gtgaaggaat ctcaggccag
     2461 ggagttcatc atagcgaccg aaaccggaat gctctacaag ctgaggaaag acaaccctga
     2521 taagacgttt ttaccggcga gggagaacgc cgtctgcaag tacatgaaga tgattactct
     2581 cgagaaggtc ctccgctcgc tcgaagat
//
LOCUS       DASSJH010000404         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1503487, whole genome shotgun sequence.
ACCESSION   DASSJH010000404 DASSJH010000000
VERSION     DASSJH010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503487"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VFJ67_08890"
     CDS             <1..1540
                     /locus_tag="VFJ67_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HET7290538.1"
                     /translation="EPSERTEEVGKEFLRVADFLTEGNIDEREAYENLRKLGFRNPDI
                     AMELFSKLLDPRRGALTREGRVTTRRVIPAFLGSILRSPDPDSALVNLERFISGIGWR
                     TSIYAVLLENPDIIELLSKLFSTSGYLSSFLIRHPEYLDVITLRSVRTEFSSKEDMIG
                     ELRSALSDESDYGGKLDAIRRFRHVETLKICLRDLNGEVDPFYVGWYLSLVAEAVLEV
                     GLALAWENAAGKSDQKRNGPRMIVLGMGKLGGRELSYNSDLDVIFIYDGKAEEHEFYS
                     KLGQKVISVLSVPTGEGLAYKIDMGLRPSGRSGALVTSFGAFRKYQEESAQVWERQAL
                     LRARPSAGDTRLGKRVADAVTELVYGRPVPPGFRKEIKHLRARMETELARESVQKLNI
                     KTGRGGIVDIEFLVQMLQLRHGGEHMEVRGQNTLDALGGLRDADIIKEKEYTALSDGL
                     FFLKRMENLLRLLHDRSINELYESDFEKLSTELGMEPGGKELKEKYLATTNTIRKIYD
                     RYFK"
     gene            1586..3004
                     /locus_tag="VFJ67_08895"
     CDS             1586..3004
                     /locus_tag="VFJ67_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7290539.1"
                     /translation="MSGFKSCIFIMADGARADVFSELLGKGELPNISRYVVERGSFRI
                     ASSVFPSTTGPAYTPYIFGKFPGRCNFPGIRWFDRSIYPDKRKLHSFRRFRSYIGLET
                     YFMNSDVSDDNTSLFEIFPRSGNILNELSRGVSMRYDKTRFSKLYYKVKGHFTDKSDE
                     VDMVARRILLQELDVMHDFIFVVFLGIDTYSHINHPFHRKVLDSYSRVDETVGLMAEK
                     LRAEGRLDETLIVIVSDHGLTQTHSHFDSLEFMNSRGYKTFYYPNVFRHFTDADAACL
                     ISGNAMANIYVKSRDGWGRKSTFEELYGLAERILERDEVDIVAGLDEMGRARIKSTRG
                     EAAAWIDGEGLINYEKISGDPFGYNGLPGRMSAEEALDLSFDTRYPDALLQIIQLLEA
                     PRTGDLVLSASPGYDLRAKHENPEHRSSHGALFREHMLVPVAMSAAIGKEHMRTVDIY
                     PTILSLMGKPMPGGLDGADLSR"
BASE COUNT          751 a          814 c          956 g          587 t
ORIGIN      
        1 cgagccctct gaaaggacgg aggaagtcgg gaaagagttt ctcagggttg cggatttcct
       61 cacggaaggg aatatcgacg agcgggaagc gtacgagaac ctgagaaagc tcggattcag
      121 gaacccggac atcgccatgg agcttttctc gaagctgctc gaccctagaa gaggcgcgct
      181 cacgcgggag ggacgcgtaa ccacgaggcg cgtcataccg gcgttcctcg ggagcatatt
      241 gaggtctccc gacccggact cagcactcgt aaacctggag cgcttcatat cggggatcgg
      301 atggaggact tcgatctacg cggtactcct cgagaacccg gacataatcg agctcctctc
      361 gaagctcttc tcaacgagcg ggtatttatc cagcttcctg ataagacatc ccgagtatct
      421 ggacgtcatc actctccgga gcgtgaggac ggagttctct tcgaaggaag acatgatcgg
      481 cgagctccgg tcggctcttt cagacgaaag cgattacggg ggaaagctcg acgctataag
      541 aaggttcagg cacgtggaga cgctcaagat ctgcctccgg gacctgaacg gtgaagtcga
      601 tcctttttat gtcggctggt acctttctct cgtggctgag gctgtgctcg aggtagggct
      661 cgcgctcgcg tgggagaacg cggccggaaa gtccgaccag aagaggaacg gcccgagaat
      721 gatcgttctc ggcatgggca agctcggagg gcgcgagctc agttataatt ccgatctcga
      781 cgtgatattc atatacgacg gcaaagcgga ggagcacgag ttctattcca agctcgggca
      841 gaaggtgata tcggtgctct ccgtcccgac gggcgagggt ctcgcataca agatagacat
      901 ggggctcagg ccctcggggc gctcgggcgc gctcgtcacg tcgttcggcg cgttcaggaa
      961 ataccaggag gagagcgcgc aggtatggga gaggcaggcg cttctcaggg cgaggccctc
     1021 ggcgggggat acgaggctcg ggaagcgcgt tgcggatgcc gtcacggagc tcgtttacgg
     1081 caggcccgtc cctcccggat tccggaagga gataaaacac ctccgcgcga ggatggagac
     1141 ggagctcgcg cgcgagagcg tgcagaagct caacatcaag acgggcaggg gcggcatcgt
     1201 ggacatagag ttcctcgtcc agatgctcca gctcaggcac ggaggggagc acatggaagt
     1261 gagggggcag aacaccctcg acgccctcgg cgggctcaga gacgcggaca taataaaaga
     1321 aaaggagtac acggcgctct cggacggctt gttcttcctc aagaggatgg agaacctcct
     1381 caggctactt cacgacaggt ccataaacga attatacgag agcgatttcg agaagctctc
     1441 gacggagctc ggcatggagc caggcggaaa agagctgaag gaaaaatatc ttgcgacgac
     1501 gaatacgata cggaagatat acgacagata tttcaaatga aacatgtgtc ggtgataatc
     1561 aaattaccag aggatcgata aaatcatgag cgggttcaag agctgcatat tcataatggc
     1621 cgacggggcg agggccgacg tcttctcgga gctcctagga aaaggagagc tgccgaatat
     1681 ctcgagatat gtcgtcgaaa gaggctcgtt cagaatagcg tcgtctgtat tcccatcgac
     1741 tacagggccc gcatacacac cttatatatt cgggaagttc cccggcaggt gcaacttccc
     1801 cggcatcagg tggttcgaca ggagtattta ccctgacaag cggaagctcc actcgttcag
     1861 gcggttcagg agctacatcg ggctagagac ctatttcatg aacagcgacg tgtctgacga
     1921 caacacgagc ctcttcgaga ttttcccccg gagcggcaat atcctgaacg agctatcgcg
     1981 cggagtgagc atgagatacg acaagacgcg gttctccaag ctctactaca aggtgaaagg
     2041 ccatttcacg gacaagagcg acgaagtcga tatggtcgcg aggcgtattc ttctccaaga
     2101 gctcgatgta atgcacgatt tcatattcgt ggtattcctg ggcatagata cgtattctca
     2161 catcaaccac ccgttccaca gaaaggttct cgactcctat tcgagggtcg atgagacggt
     2221 agggctgatg gcggaaaagc tcagggcgga agggaggctc gacgagactc tcattgtcat
     2281 cgtgagcgac cacggactca ctcagactca ttcccatttc gattcgctcg agttcatgaa
     2341 cagccgggga tacaagacgt tttactaccc gaacgtattc agacatttta cagacgccga
     2401 cgctgcgtgc ctcatctccg ggaacgccat ggccaacata tacgtgaaga gccgggacgg
     2461 gtggggtaga aaatccacgt tcgaggagct gtacggtctc gcggagagaa tcctcgaaag
     2521 ggatgaggtg gacatagtcg ccggactcga cgagatgggc agggcgagga taaagagtac
     2581 gaggggggag gccgcggcct ggatcgacgg ggagggtctc atcaactatg agaagatttc
     2641 gggggacccg ttcggatata acgggcttcc cggcaggatg agcgcggaag aggcgctcga
     2701 cctgagtttc gatacgcgtt atccggacgc tctccttcag ataatccagc tcctcgaagc
     2761 tccgcgcacc ggggacctcg tcctgagcgc gagcccgggc tacgacctca gggcgaagca
     2821 cgagaatccg gagcaccgct cctcgcacgg cgcgcttttc agagagcaca tgctcgtgcc
     2881 cgtcgctatg agcgccgcga tcggaaagga gcacatgcgc accgtggaca tatatccgac
     2941 tatattgagt ctgatgggaa aaccgatgcc tggcggtcta gacggcgcgg acctgagccg
     3001 ttgaccgagc gcggctgtta agagaagacc gacctcgcga cggcttcgga tatgaacagc
     3061 atcaccgctc cccataggaa gtagcgccag atatccctgt ataccttt
//
LOCUS       DASSJH010000405         3874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_529872, whole genome shotgun sequence.
ACCESSION   DASSJH010000405 DASSJH010000000
VERSION     DASSJH010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529872"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..732)
                     /gene="rph"
                     /locus_tag="VFJ67_08900"
     CDS             complement(16..732)
                     /gene="rph"
                     /locus_tag="VFJ67_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET7290540.1"
                     /translation="MRSDGREWDQLRPVKITRGIMKYAEGSALIETGETKVLCTATFE
                     DRVPPFLKDTGKGWVTAEYSMLPRSTKERITRDSVRGKIGGRSHEIQRIIGRALRSVI
                     DLGKLGERSITIDCDVLQADGGTRTASITGAFVALSDAALNLIRQGVISENPIVDFIA
                     AVSVGIVGGKPALDLNYEEDSKAEVDMNVAMTGSGLLVEVQGTAEGKPFSKKNLNALI
                     KLSEKGITELIEKQKEILAE"
     gene            1155..1565
                     /locus_tag="VFJ67_08905"
     CDS             1155..1565
                     /locus_tag="VFJ67_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290541.1"
                     /translation="MRNILISSVSLFIFSLCASLIGTNLNTALAGETLDGKSFSVELT
                     EANKSEASQDELIFKDGTFFSTGCEQYGFTPAKYEAKSKGGVIMFKSTLTSDKEGKSE
                     WEGAVTGDEISGTMFWTKEGQEPIIYTYKGAIKK"
     gene            complement(1713..3281)
                     /locus_tag="VFJ67_08910"
     CDS             complement(1713..3281)
                     /locus_tag="VFJ67_08910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET7290542.1"
                     /translation="MKQDILDKYVPGRFRIRKEGNVAVYNVLFDELPGVADQLYFTHS
                     LQLKTITAMDERSESGGFRIYYLFGVPKENIFLAPCITLTDREEFPSITKAIHEASGY
                     ERKIGTFFGLKPARHPHPRPIILHENWPGTSFPLRKDFEWRHRPAEAHGSYEFRKVEG
                     KGIYEIPVGPVHAGIIEPGHFRFNVLGEEIVSLEPKLGYKHKGTEKLFEVLPVAGKVR
                     LSERVSGDTSFTHSLAFCQAVENLAGIAVPERAKYLRVVFSEMERLANHLNDIGFIML
                     DAGYNFGGSSGARLREIVMQSCERIAGNRFLRGINTVGGVTRDITGETGAELLNTLKK
                     VHDDFNDVIEIAEGSFSLSNRLSGTGKLERQIALDHGAIGVTGRAIGIAHDARVEYPY
                     AAYDKLKFEIALEEEGDVRARWLVRVKEVHSSLNIIEQALLGIPLGDELISGNVTALR
                     KHSYGVGVAEGWRGDIVYFIATDAEGEISRVDVRDPSFINWTVLGYAGKGNVVPDFPL
                     INKSFNLSYSGNDL"
     gene            complement(3286..>3874)
                     /locus_tag="VFJ67_08915"
     CDS             complement(3286..>3874)
                     /locus_tag="VFJ67_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7290543.1"
                     /translation="SVAALIIFSQKNYKRLLAYSSIEHMGVVALGFGFGGAGAFAALL
                     HMIYPALTKSVLFLSAGNILVKYGSTKIRNVKGVLTVLPVTGVLFFLGFLAITGVPPF
                     GTFITEFSILSAGIKTHPTTVIITLAVLALVFVGFLRHVVSITFGNSDIPMAKGESGL
                     DTIVPIMLLVALLIVASFFIPSSVQSLLRSATINY"
BASE COUNT          851 a         1079 c         1009 g          935 t
ORIGIN      
        1 atcattccgg gcggatcatt cggcgagtat ttctttctgc ttctctatga gctcggttat
       61 gcctttttcc gacagcttta tgagcgcgtt gaggttcttt ttcgaaaagg gctttccttc
      121 agccgtgccc tgcacctcga cgagtagccc tgagccggtc atggcgacgt tcatatcgac
      181 ctccgccttg gaatcttcct cgtagttgag gtcgagcgca ggcttgccgc caacgatacc
      241 cacgctcacg gcggctatga aatcgactat cgggttctcg gaaatcacgc cctgccggat
      301 gaggttgagc gcggcgtccg agagtgcgac gaaagccccc gtaatggaag ccgtcctcgt
      361 cccgccgtcc gcctggagca cgtcgcagtc aatcgtgatc gacctctcgc cgagcttgcc
      421 gaggtctatc acggacctga gcgccctgcc tataatccgc tgtatttcgt gagacctccc
      481 cccgatcttc cccctcacgc tgtccctcgt tatcctctcc ttggtcgagc gcggaagcat
      541 cgagtactcg gccgtcaccc atcccttgcc tgtgtccttg aggaatggcg ggaccctgtc
      601 ctcgaaagta gccgtgcaga ggactttcgt ctcacccgtc tctatcaggg ccgatccctc
      661 ggcgtatttc attatccctc tcgtaatctt cacggggcgg agctgatccc attctcttcc
      721 gtctgagcgc attttcgtta acctcgttct attgttagcc tgagataata ccacatcagc
      781 gctcgtgagc gcggaaagac aggggagagt gccggggaac aaacttgcgc tccaggccgg
      841 ggctgaatag ccgagcgcgc ttttcggcag catgctcgcg gcttacctca taccgcttcc
      901 gctgcttacc tacggtataa tcgccgcgct gggaggtctg ccgcttgtta actataagga
      961 agaaaacata aactcccgca agttatgaga ttaatcccgt gtacctcccc ccccatattg
     1021 ctgagccagg ccgggctgcg cgttgcaaag cccgccgtat tgatgataaa ttgggccatt
     1081 ctgcggcatt gacatcgctt tacatactct tgtagagtta tttgcaggtg caatttcagc
     1141 aaacaggggg gaatatgaga aatatcctga tttcatcggt atctcttttt attttttcat
     1201 tatgcgcttc gttaatcggc acgaacctga atacagcgtt agccggcgaa acgcttgacg
     1261 gcaagtcttt ttcagtggag ctcaccgaag cgaataaaag tgaagcgtcc caggatgagc
     1321 tgattttcaa ggacggaacc ttcttctcca caggctgcga gcagtatggc tttacacccg
     1381 ccaagtatga agcgaagtcg aaaggcggcg tgataatgtt caaatccacg ctaacgagcg
     1441 ataaagaagg aaagtcggaa tgggaaggcg cggttaccgg agacgaaatc tcgggcacta
     1501 tgttctggac gaaggaaggc caggaaccga ttatatacac ctataaaggc gctataaaaa
     1561 aatagatcgt taagacgacg gggacgaaag gcacgggacg gggagctttc gggagccccg
     1621 gtatctccct cgcgcggttg agagcgtcgt caatgttccc gaatgcgaag aacggctgag
     1681 ttgactggct gtttatccgt gtacggacgg ttctacaagt cgtttccgga ataagagaga
     1741 ttaaagcttt tattgatgag agggaaatcc ggtaccacgt tccccttccc cgcgtagccc
     1801 aatacggtcc agtttatgaa tgaagggtcg cgcacgtcta ccctgcttat ctctccttcc
     1861 gcatccgtag ctataaaata gacgatatcg ccccgccagc cttccgctac tccgacgccg
     1921 tagctgtgtt tcctcaatgc cgttacattt ccggatatca attcgtcacc caaagggata
     1981 ccgaggagcg cctgctctat gatattcagg gaagaatgca cctccttgac gcggacgagc
     2041 catcttgccc gcacgtcccc ttcttcttcg agggctatct cgaattttaa tttgtcgtat
     2101 gcggcatacg ggtattccac ccgtgcatcg tgggcgatcc ctattgccct gccggttacg
     2161 cctatggctc catggtcgag ggctatctga cgttcgagct ttcccgtccc ggaaagccgg
     2221 ttcgagaggg agaagctgcc ttcggctatt tcgattacat cgttaaaatc atcgtgcact
     2281 tttttgagtg tgtttagcag ctccgcgccc gtctcgcccg ttatatccct cgttacgccg
     2341 cctacggtat taatgccccg caggaagcgg tttccggcga tgcgttcaca ggattgcatg
     2401 acgatttcgc ggaggcgagc tccgctgctg ccgccgaaat tgtatccggc gtcgagcatg
     2461 atgaacccga tgtcgttcaa atggttcgca agtctctcca tctcggaaaa tacgactctc
     2521 aaatacttcg ccctctccgg gaccgctatg ccggccaggt tttccacggc ctgacaaaat
     2581 gccagagaat gagtgaacga agtgtctccc gaaacccttt cggataacct gaccttgcct
     2641 gcgaccggca ggacctcgaa gagcttttcc gtgcccttgt gtttatatcc cagcttcggt
     2701 tcgagtgata cgatttcttc tcccaaaaca ttgaaccgga agtgtccagg ttcgataatg
     2761 ccggcgtgga cgggcccgac cgggatctcg taaataccct tcccttcgac cttccggaat
     2821 tcatacgatc cgtgcgcttc cgccgggcgg tgccgccatt cgaaatcttt tctcagcggg
     2881 aaagacgtgc cgggccaatt ttcatgcagt atgatgggcc gcggatgcgg gtgcctcgcc
     2941 ggctttagac cgaaaaacgt cccgatcttc ctttcgtacc cggaagcttc gtgtatagct
     3001 ttggtgatcg agggaaactc ctcacggtcc gtaagcgtta tgcagggagc gaggaaaata
     3061 ttctctttcg gcacgccgaa caaataatag atcctgaacc ccccgctctc gctcctctcg
     3121 tccatggcgg ttatggtctt gagctgtaat gagtgagtga aatagagctg gtcggctacc
     3181 ccgggcagtt catcgaagag gacgttatag acggcgacgt tgccttcttt tctgatcctg
     3241 aatctgcccg gaacgtattt atctaatata tcttgtttca tggtgtcagt aatttattgt
     3301 cgccgagcgc agcagcgact gcacgctcga cggaatgaaa aagctcgcga cgatcaggag
     3361 agcgaccaga agcataattg gaacgatcgt gtccagtccg ctctcgccct tggccatggg
     3421 gatgtcgctg ttcccgaacg tgatcgagac tacgtgccgc agaaaaccga cgaatacgag
     3481 cgcaagcacg gctaacgtaa taattacggt cgtcggatgt gtcttgatgc ccgcggacag
     3541 tatcgaaaat tctgttataa acgtcccgaa cggaggaacg ccggtaatcg ccaggaatcc
     3601 caggaaaaac aggacgcccg tcacgggtaa gaccgtcaga acgcctttca cgtttcttat
     3661 ttttgtggaa ccgtatttca ccagaatgtt gcccgccgat aaaaacagga ccgattttgt
     3721 aagcgcgggg tagatcatat gaagaagcgc agcgaacgcc cccgcgcctc cgaacccgaa
     3781 ccccagggcg acaacgccca tgtgctcaat actggaataa gcgagcagcc gtttatagtt
     3841 tttctggctg aagatgatca gcgcggcgac ggat
//
LOCUS       DASSJH010000406         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1279672, whole genome shotgun sequence.
ACCESSION   DASSJH010000406 DASSJH010000000
VERSION     DASSJH010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279672"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFJ67_08920"
     CDS             complement(<1..765)
                     /locus_tag="VFJ67_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET7290544.1"
                     /translation="MSLKKSNTEKYAPDTPLHSVTFSEALRVWVRVAVHSFGGPAGQI
                     AVMHRILVEEKRWISESRFLHALNYCMLLPGPEAQQLATYIGWLLHKTRGGLTAGSIF
                     VLPGFISIMALSILYAEFQDTTFVEALFFGLKPAVMAVVVEAVIRIGKRALKNNVMVS
                     LAGLAFIAIFFFNVPFPLIVLCAGLTGFIGGKLREDLFYVIRGHGSQSGGQSGDEDGF
                     VITDGVASHTNPSLSRTLKVIAVWTPLWLGPVAALIA"
     gene            958..1920
                     /locus_tag="VFJ67_08925"
     CDS             958..1920
                     /locus_tag="VFJ67_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET7290545.1"
                     /translation="MVLFLGEDDVRQILTMSDAIKVLEETFRQQGLGNIINQPRQRVR
                     TDVSMLHYLAGALPHLGVMGYKAYTSSRAGIKFRVFLHDMETGELLSIMDGNYMGMMR
                     TGAVSGVATKYMAREDMNRVCIYGSGWQARGQLLAAAAVRNVKKVSVYSRNAAQRETF
                     SKEMEKQMRRTVTPVSSAEEAAKDADCVITATTAFEPVFKGEWLAKGTHVNAIGGNFL
                     FKREIDERTVRSADVIAVESLEQTKIEAGEFMPLVEKGTLRWSQIAELGDVVAGKTSG
                     RRSGEDITLFKSLGIAVEDIAVAAHVYKVAKGAGIGVRLDMPSN"
     gene            1983..2837
                     /gene="mtnP"
                     /locus_tag="VFJ67_08930"
     CDS             1983..2837
                     /gene="mtnP"
                     /locus_tag="VFJ67_08930"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET7290546.1"
                     /translation="MKIVGVIGGSGLYALEGITEVETVSVNTPWGKPSDDPVVGELGD
                     VRLIFLPRHGKGHKLMPSEINYRANIFAMKMLGAERIVSVSAVGSMREDIKPGHVVIP
                     SQFYDHTKSRASTFFGDGIAAHVSMADPVCPVLSESLYHAALSAGATAHRDATYICME
                     GPQFSSRAESNIYRKWGVDVIGMTNMPEAKLAREAEICYSTLALSTDYDCWHEHHGDV
                     TVDDIMETLMSNVALARKIIVNVLSAISETRDCACKASLENAIITSRDAIAPEVKERL
                     GILIKRYV"
     gene            2837..>3591
                     /locus_tag="VFJ67_08935"
     CDS             2837..>3591
                     /locus_tag="VFJ67_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7290547.1"
                     /translation="MKLLVVGSIALDTVETPFGKEENILGGSASYFSLAASMFTDVCA
                     VAVVGKDFPEEHLDLFRSKGIALNGVKREDGNTFRWEGKYGYDLGDPETLGTHLNVFE
                     NFKPVLPEEYRNIEYVFLANIDPDLQLSVLNQIKKPKLVACDTMNYWIENKLDALKEV
                     IKRVDILMLNDSETRILAKEPRITHAARAVLGLGPKALIVKRGEYGALMFSKEGIFWA
                     PSYPLEEVIDPTGAGDSFAGGFMGFIAGQKIDD"
BASE COUNT          860 a          896 c         1133 g          702 t
ORIGIN      
        1 agctatcagg gccgcgacag ggcccagcca gaggggggtc catacggcga ttactttgag
       61 tgtgcgcgaa agggatgggt tcgtatggct cgccactccg tccgttatga cgaaaccgtc
      121 ctcatcgcct gattgtcctc cgctctgaga gccgtgtccc ctgatgacgt agaatagatc
      181 ctcacggagc ttccctccta tgaagccagt caggcccgcg cacaggacga tcagggggaa
      241 gggtacgttg aagaaaaata tggcgatgaa ggctagtccc gcgagggaga ccatcacgtt
      301 atttttgagc gcgcgttttc ctattcgtat cacggcctcg acgactacgg ccattaccgc
      361 gggcttgaga ccgaagaaaa gcgcttctac gaaagtcgtg tcctgaaatt cagcgtagag
      421 gatgctcaat gccatgatgg agataaatcc ggggaggacg aagatgctcc ctgccgtcaa
      481 gccgccgcgg gtcttgtgga ggagccatcc gatgtaggtt gcgagctgct gtgcttcggg
      541 accgggaagg agcatgcagt agttgagtgc gtggaggaac ctgctttcgc ttatccacct
      601 cttttcctca acaagtatcc ggtgcatgac cgcgatctgc cccgcgggcc cgccgaagct
      661 gtgaacggcg accctgaccc agacgcgcag tgcttcactg aatgtgacgg agtggagtgg
      721 agtatcaggt gcgtattttt ccgtgttgct ttttttcagg ctcatggctc cttttcgttg
      781 aattctatac cgtacaattg aattcgatct tacacaataa ttgtaaattg gccatgaact
      841 ccgagtattc ttcataactc cccagccttc ctttaaaatc aggctcttaa atgttaaaat
      901 cctacccggg agaggcggcc tgacccgcgc gggtcgggta tttaaacgga gggctagatg
      961 gtcttattcc tgggagagga cgacgtcagg cagatcctca ccatgtcgga cgccataaag
     1021 gtgcttgaag agactttcag gcagcagggg cttggaaaca tcattaatca gccgaggcag
     1081 agggtcagga cggatgtgag catgctccat tacctggcgg gggcccttcc gcatctgggt
     1141 gtcatgggct ataaggccta cacgtcctcg agagcgggca taaagttcag ggtctttctg
     1201 cacgatatgg agaccgggga gcttctctcc attatggacg ggaattacat gggaatgatg
     1261 aggaccggcg cggtctccgg cgttgcgacc aagtatatgg cgcgggagga catgaaccgt
     1321 gtctgcatat acgggtccgg ctggcaggcg aggggacagc tcctggcggc cgcggcggtg
     1381 aggaacgtga agaaggtatc cgtatacagc aggaacgccg cccagaggga aacgttctcg
     1441 aaagagatgg aaaagcagat gcgtagaacc gtaactcccg tctcgtcagc ggaagaggcg
     1501 gctaaggacg cggactgcgt cattacagcg actacggcgt tcgagcccgt atttaagggc
     1561 gaatggctcg cgaaagggac acacgttaac gcgataggcg gtaatttcct tttcaagagg
     1621 gagatagacg agaggacggt gagatcggca gacgttatcg cggtcgagtc gctcgagcag
     1681 acgaagatag aggcggggga attcatgccg ctcgtcgaaa aggggacgct caggtggtct
     1741 cagatcgccg agctcggtga cgtggtcgcg gggaagacga gcgggaggag gagcggcgag
     1801 gacataacgc tcttcaagtc gctgggcata gccgtcgaag atatcgcagt cgccgcgcac
     1861 gtgtacaagg tcgcgaaggg ggcgggcatc ggagtgagac tcgacatgcc gtctaactga
     1921 gaccgcagga ccggaacatc catgttgata gattaaagtt aatttcgaat gcagaggcga
     1981 aaatgaaaat agtcggagta ataggaggga gcgggctgta tgccctcgaa ggaataaccg
     2041 aggtcgagac ggttagcgtg aatacgccat ggggaaagcc ttcggacgac cccgtagtag
     2101 gcgagctggg ggatgtcagg ctcattttcc tgccgaggca cggaaagggg cacaagctca
     2161 tgccgtccga aattaactac cgggcgaaca tattcgccat gaagatgctc ggggccgagc
     2221 gtatcgtatc ggtgagcgcg gtcggaagca tgagggagga tatcaagccc ggccacgtag
     2281 tcataccatc gcagttctac gaccatacca agtccagagc gtcgacgttc ttcggggacg
     2341 ggatagccgc gcacgtgtcc atggccgacc ccgtatgccc ggtgctgtcg gaatcgctgt
     2401 atcacgccgc gctctcagcc ggcgccacgg cgcacaggga cgcgacttac atctgcatgg
     2461 aaggccccca gttctcctcg cgcgccgaga gcaacatata caggaagtgg ggggtggacg
     2521 taatcggaat gacgaacatg ccggaggcca agcttgcgcg cgaagccgag atttgctatt
     2581 ctacactggc attatcgacc gactatgact gctggcacga gcatcacggc gatgtcacgg
     2641 tggatgacat tatggaaacg ctcatgagca acgtggctct cgccaggaag ataatcgtaa
     2701 atgtgctctc cgctataagc gaaacgaggg actgcgcctg taaggcgtcg ctcgagaacg
     2761 ccattataac atcacgcgac gcgatcgcgc ccgaggtaaa agagagactc ggaattctca
     2821 taaagaggta cgtataatga agcttctggt agtcggatcg atagctcttg atacggtgga
     2881 gactccgttc gggaaagagg agaacatact cggagggtcg gcgtcctatt tctcgcttgc
     2941 ggccagcatg ttcacggacg tgtgcgccgt agcggtggtc ggaaaggatt ttccggagga
     3001 gcacctcgac ctcttcagat ctaaggggat agcgctgaac ggggtaaaga gggaagacgg
     3061 caacacgttc aggtgggagg ggaagtacgg atacgacctc ggagacccgg agacgctggg
     3121 gacgcacttg aatgtgttcg agaacttcaa acccgtcctg cccgaggagt acagaaacat
     3181 agaatatgtg ttcctagcca acatagaccc cgatctccag ttgagcgtgc tgaatcagat
     3241 caaaaaaccg aagctcgtag catgcgatac gatgaattac tggatcgaga acaagctcga
     3301 tgccctcaag gaagtcataa agcgcgtgga tattctcatg ctgaacgact ccgagaccag
     3361 gatactcgcg aaggagccgc ggatcactca cgcggccagg gcggtcctcg ggctcggccc
     3421 caaggcgctc atcgtgaaga ggggagagta cggggcgctg atgttctcca aggagggcat
     3481 attctgggcg ccaagctacc cgctcgaaga ggtgatagac cccacggggg cgggggactc
     3541 gtttgcgggc gggttcatgg ggttcatagc cggacagaaa atcgacgacc a
//
LOCUS       DASSJH010000407         5613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_747352, whole genome shotgun sequence.
ACCESSION   DASSJH010000407 DASSJH010000000
VERSION     DASSJH010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747352"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..3294
                     /locus_tag="VFJ67_08940"
     CDS             181..3294
                     /locus_tag="VFJ67_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7290548.1"
                     /translation="MVDFFIQRPVLATVLAILITLIGAISIPILPIAQFPDIAPPTVN
                     VSATYTGASAEVVETTVTDPIEEQINGVEGMTYMESYSSNDGTMNINVTFDVGYDLDI
                     ANVDVTNQVQLATPLVPEEVGKYGISVEKQSPSLILAVHLISPDGSRDELFLSNYIGI
                     QIYQTLQRIPGVGNLTIWGEREYSMRIWLNPDKIASLGLTVADVIDAISEQNLQVAAG
                     SIGEPPFPEGQQFQYTITTLGRLETVEEFGDIIIRAETDGTVVRIKDVARVELGAQSY
                     DQDSLLDGEPAIGIGIYQLPGANALDVDKQVRATMEQLKKNFPSGVDYRIVYDTTEFV
                     TASITEVIKTLFEAFILVFIVVFVFLQNFRATLIPAITIPVSLIGTFALINAFGFSIN
                     TLSLFGLVLAIGLVVDDAIVVVENVSRLLEEKDMSPKEATSAAMKEVTGPIVATSLVL
                     MAVFVPVSFMPGISGQMYRQFALTIAFSVGISAINALTLSPSLCALFLRKEKGEKKWW
                     FYRKFNEGFDRFRNGYQGWVKELTARWKLVVLVFIVLMGLTAYMFKIVPTGFVPEEDQ
                     GYFIVNIQGPEGSSLQRTNATMKQVYEMLMDTPGIAHVVSISGLNFLTSSSDSNSGAM
                     FPVLAPWSQRKSKETQIDYILASVRKKFAGIQGAEVVAFNAPAIQGLSSTGGFQFELE
                     DRTNLGLETLQKTMGEIIQAGQKRPELVGLFAYFSVDTPGLYIELDRTKTKTQGVSIS
                     EVFQVLQSYLGSLYVNDFNLFGQVYKVFIQADMQYRNTIDDISRLYVRTIRGDLVPLD
                     TLINVTQIVGPETITHYNLYRSAEIDGDNAPDYSSGQAITAMEELADKYMPEGMGFEW
                     SGISYQEIKAGNLAPLIFGLSLVFVFLFLAALYESWAMPFMVMLAVPLALLGAMSAQW
                     LRGLQDDIYCQIGLVMLIGLASKNAILIVEFAKQRREEGMPIQEAAMTAALIRLRPIL
                     MTAFAFILGVFPLVVASGAGANSRHSLGTAVFGGMIVSTLLSLIIVPVFYVIIENLRE
                     RGSSKPA"
     gene            complement(3492..4142)
                     /locus_tag="VFJ67_08945"
     CDS             complement(3492..4142)
                     /locus_tag="VFJ67_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA lyase"
                     /protein_id="HET7290549.1"
                     /translation="MKKDLCEKLRISYTEKRGIIRARLDEFRQVYSGADDGRIFEELA
                     FCICTAGASAKMGLRSIDALRDILLEGSLRKFRKRLEGIHRFPNYRPAYIIHTREYLR
                     REHGLRLRDLIESFHDPFERRDFFARNKDIKGIGYKESSHFLRNIGYGGYAILDKHIL
                     NTLSELGVIASPKPPGTRDRYIAIEDSLRRFADDIGIPMDELDLLLWSEKTGEVLK"
     gene            complement(4200..>5613)
                     /locus_tag="VFJ67_08950"
     CDS             complement(4200..>5613)
                     /locus_tag="VFJ67_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7290550.1"
                     /translation="TVTSQKSKELYALADVSPTIAQRSTGGTHRVRRGETLGRIASRY
                     GVSVSSIKRANGMRSSTIRTGQVLRIPGLSGPSSSYASADDGGTTVKYRVRRGDTLGA
                     IAARHRVSIASIKNANSLRSSMIRSGQVLTIPGSTGRYYETEETASNSTRIAKHRVQP
                     GETLGGIAAKYNVSISTLKRANEIKGTTIMSGQELDIPYIAGGTYVASSDAAYEADPE
                     PAVTKSTTSRTPAPRAQARYTVKSGDTLGKIASDHGVSMASIQSANNMSGTVVKRGQV
                     LTIPGSSAAASETVTAERTNYSASGSRYTVVRGDTLGSISRKFGVSVASIQNANNMNG
                     SVVKYGQVLTIPGTASASSTTRETASAGTGKYRVQPGDTLGSIAEKYDVGVSSLMSAN
                     NMNGSTIRSGQVLVIPGGNYASSSSSSAGDDVINYRVKKGDTLWKIASRHNVSVASIQ
                     KWNNLRSAELTPGVSLTIYK"
BASE COUNT         1144 a         1611 c         1528 g         1330 t
ORIGIN      
        1 gacggggtca aagcgggtga gaaggtaata acggagggcc tgcagaaggt aaagccgggc
       61 atgaccgtcg tgcctgagac ggcctccgaa gaatcggaga aatccgctcc cgagccgtcg
      121 ccgtcgggtg caaagggcaa gtaacacggc gcccctggta caaagttaag gagatttact
      181 ttggttgatt tttttataca gcgtccggtc ctcgccacag ttttagcaat actcattacc
      241 ctcatcggcg ctatttccat acccatactg cctatcgcgc agttcccgga catcgcaccc
      301 cctacggtca acgtttcggc gacttatacg ggcgcgagcg cggaggtcgt ggagacgacc
      361 gttaccgacc ctatcgaaga acagataaac ggcgtcgagg gtatgaccta catggagtca
      421 tacagctcga acgacgggac catgaacatc aacgtcacct tcgatgtcgg ctatgacctc
      481 gatatcgcga acgtggacgt aacgaaccag gtacagttag cgacgccgct cgtccccgaa
      541 gaggtgggca agtacggcat atctgtggaa aaacagtcgc ccagcctcat cctcgccgtc
      601 cacctgatat cccccgacgg gagccgcgac gagctgttcc tgagcaatta tatcggcatc
      661 cagatatacc agacgcttca gcgaatcccc ggagtgggaa acctgacgat atggggcgag
      721 agggaatatt ccatgcgcat ctggctcaac ccggacaaga tcgcgagcct ggggctcacg
      781 gttgcggacg tgatagacgc catatccgag cagaacctcc aggtggcggc gggctctata
      841 ggcgagccgc cttttccgga aggccagcag ttccagtaca cgataacgac gctcggaagg
      901 ctcgagacag tcgaggagtt cggagacata atcatacgcg ccgagacgga cggcaccgtc
      961 gtcaggatca aggacgtggc ccgggtcgag ctcggcgcgc agagctacga ccaggacagc
     1021 cttctcgacg gcgagcccgc gataggcata ggtatttatc agctcccggg cgcaaacgcc
     1081 ctcgacgtcg acaaacaggt cagggccacg atggagcagc ttaaaaagaa cttcccatcg
     1141 ggtgtcgact acaggatcgt ttatgatact accgaatttg taacagcgtc cataaccgaa
     1201 gtgataaaga ccctcttcga ggcctttata ttggtgttca tagtggtttt cgtgttcctc
     1261 cagaacttca gggctacgct cattcccgcg atcaccatac cggtgtcatt gataggcacg
     1321 ttcgccctca taaacgcttt cggattttcc ataaacactc tcagtctatt cggactcgtc
     1381 ctcgcgatcg gactcgtcgt cgacgacgcc atagtggtcg tggaaaacgt ctcccgtctc
     1441 ctcgaggaaa aggacatgtc ccctaaggag gcgacgagcg cggccatgaa agaggtgacg
     1501 ggacccatcg tcgcgacatc gctcgtgctc atggcggttt tcgttcccgt atccttcatg
     1561 ccgggaattt cggggcagat gtaccggcag ttcgctctca cgatcgcgtt ctctgtgggc
     1621 atatccgcta tcaacgccct cacgcttagc ccctcgcttt gcgccctctt tctccggaag
     1681 gaaaaaggcg agaagaagtg gtggttctac aggaaattca acgaaggttt cgaccgcttc
     1741 aggaacgggt atcagggctg ggtgaaggag ctgaccgcca ggtggaagct cgtggtgctc
     1801 gtattcatcg tgcttatggg tttgacggct tacatgttca agatcgtgcc cacgggcttc
     1861 gtccccgagg aagaccaggg ttacttcatc gtaaacatcc aggggcccga gggctcgtcc
     1921 ctccagcgta caaacgccac gatgaaacag gtctacgaaa tgctcatgga tacgcccggt
     1981 atcgcgcacg ttgtatccat ctccggactt aacttcctca cctcgagctc cgactcaaac
     2041 tcgggggcca tgttcccggt tctggcgccc tggagccaga ggaagtcgaa agaaacccag
     2101 atcgactaca tactcgcgag cgtgcgtaag aagtttgcgg gtatacaggg cgccgaggtg
     2161 gtcgcattca acgcccctgc aatacagggt ctgagctcga cgggcggttt tcagttcgag
     2221 ctcgaggaca ggaccaacct cggccttgag acccttcaga agacgatggg cgagataatt
     2281 caggccgggc agaaaaggcc ggagctcgtg ggtctcttcg cttatttctc ggtcgatacg
     2341 ccgggtctat atatcgagct cgaccggacc aagaccaaga cccagggggt atcgatatcg
     2401 gaagtattcc aggtcctcca gtcctatctg ggttcacttt acgtgaacga tttcaacctg
     2461 ttcggtcagg tctacaaggt gttcatacag gcggacatgc aataccggaa cactattgac
     2521 gacatatccc gtctttacgt gcgcaccatt agaggggatc tcgtaccgct cgatacactg
     2581 attaacgtga cccagatagt cggtcccgag acgataaccc attataatct ctaccgctcg
     2641 gccgagatcg acggtgacaa cgctcctgac tacagctcgg gtcaggctat aacagcaatg
     2701 gaagagctcg cggataaata tatgcccgaa ggtatggggt tcgaatggtc gggcatctca
     2761 tatcaggaaa taaaagccgg gaacctcgcc cctctcatat tcggtctgtc tctggtattc
     2821 gtgttcctct tcctcgccgc gctttacgag agctgggcga tgccgttcat ggtcatgctg
     2881 gctgtgccgc tcgcgctctt gggcgccatg tcggctcaat ggctgcgcgg actccaggac
     2941 gacatctact gtcagatcgg tctcgtcatg ctgataggac tcgcgagcaa gaacgcgatc
     3001 cttatagtcg aattcgcgaa acagcgccgc gaggagggta tgcccattca ggaggccgcg
     3061 atgacggccg cgctcatcag gctgaggcct atcctgatga ctgctttcgc gtttattctg
     3121 ggagtattcc cgctcgtcgt cgcttcgggt gcgggtgcca acagccgcca ttcgctcgga
     3181 acggcagtct tcggcggcat gatagtttca acgcttctca gcctcatcat cgttcccgta
     3241 ttctacgtga taatcgagaa cctgagggag cggggatcga gcaaacccgc ttagtgcgca
     3301 cacaatgtgt gccgatatat ggcactattg gacctcttcc gcttaagcca ataatgacgt
     3361 cctccccgtc cgttgatagt tacggcgtag cttaagcgca tgcccggcga agttttgacg
     3421 aaaccgggaa gccggaagct tgtcgaagcg ctgaacatct attgatccgg cctgtccgag
     3481 gcgacccgcc gctatttcag cacctctccc gttttctcgc tccacagcag aagatcgagc
     3541 tcgtccatgg ggatgcctat atcgtccgcg aaacgcctga gactgtcctc gatagctata
     3601 tacctgtccc tcgtgccggg aggcttcggg ctcgcgatca ctccgagctc tgagagcgta
     3661 ttgagtatgt gcttgtcgag tatagcgtaa cccccatagc ctatattcct caggaagtgg
     3721 ctcgactcct tgtacccgat gccttttatg tccttgttcc tcgcgaagaa atccctcctt
     3781 tcaaaaggat catgaaagga ttctatgaga tctctaagcc tgagcccgtg ctcgcgtctc
     3841 agatactctc tcgtgtgaat tatgtatgcc ggcctgtagt tggggaacct gtgtatgcct
     3901 tcgagtctct tcctgaattt tctcaggctc ccttcgagca gtatgtccct cagggcgtcg
     3961 atggatctca gccccatctt tgcgctcgcc cccgccgtgc aaatacagaa ggcgagctcc
     4021 tcgaatatcc gtccgtcgtc tgcgccactg taaacctgcc ggaattcatc gagcctggcc
     4081 ctgatgattc ctctcttctc ggtgtaggaa atcctcagct tttcacacag gtcttttttc
     4141 atttgcatta agccgggcgg tgtactgcaa gtaatcgggt gttagaaggt taacaggtat
     4201 tatttgtaga tagtcaggct gactccggga gtcagctcgg ccgatctcag gttgttccac
     4261 ttctgtatgc ttgccacgga tacgttgtgc ctgctcgcta tcttccagag cgtgtcgcct
     4321 ttcttgaccc tgtagttgat aacgtcgtcc cccgcggaag atgaagacga ggatgcataa
     4381 ttcccgccgg ggattacgag cacctgtccc gaccttatcg tagagccgtt catgttgttg
     4441 gcgctcatta gcgaagacac tccgacatcg tacttttccg ctatgctgcc gagcgtgtca
     4501 ccgggctgga ctctgtactt tcccgtgccc gcgctcgcgg tctccctagt tgtggacgac
     4561 gcgctcgctg ttccggggat ggtaagcacc tgtccgtact tgactacaga gccgttcatg
     4621 ttgttggcgt tctgtatgga tgcgacggac acgccgaact tgcgggaaat acttcccagg
     4681 gtatctccgc gcaccaccgt gtacctgctg cccgatgcgc tgtaattcgt tctttcagcc
     4741 gtcacggttt cagaagctgc cgcggagctt ccgggaatgg ttaaaacctg tccgcgtttt
     4801 accacggtgc cgctcatatt gttcgcgctc tgtatggagg ccatggatac gccgtgatcg
     4861 gacgctatct tcccgagtgt gtcgcccgat ttgaccgtat atcgcgcctg agcgcgaggg
     4921 gcgggagtcc ttgacgttgt tgactttgtg acggcgggtt caggatcggc ttcgtatgcc
     4981 gcgtcgctgc tcgcaacata tgtgccgccg gcaatatatg gtatatccag ctcctgtccg
     5041 gacattatcg tcgtgccctt gatttcgttc gcccgtttta gagtagatat gctgacgtta
     5101 tatttcgccg cgatgccgcc gagagtctcc cccggctgaa ctctatgctt ggctattctc
     5161 gtggaattgc ttgccgtttc ctcggtctcg tagtaacggc cggtagatcc gggaatcgtc
     5221 agaacctggc ctgatcgtat catcgagctc ctgaggctgt ttgcgttctt tatcgaagct
     5281 atgctcaccc tgtgcctggc ggctatcgcc cccagcgtgt ctccgcgcct taccctgtat
     5341 ttaacggtcg taccgccgtc atcagccgac gcgtacgagc tcgatgggcc ggagaggccc
     5401 gggattctta acacctgtcc cgttcttatc gtcgagctcc tcatgccgtt tgcccttttt
     5461 atgctcgaca cgctgacccc gtaacgggac gctatcctgc cgagggtctc gcctctcctt
     5521 accctgtgag tgccccctgt actcctctgt gctatcgtcg ggctaacgtc tgcgagcgca
     5581 tatagctcct tgcttttctg ggaggtcacg gtc
//
LOCUS       DASSJH010000408         5537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_954646, whole genome shotgun sequence.
ACCESSION   DASSJH010000408 DASSJH010000000
VERSION     DASSJH010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954646"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFJ67_08955"
     CDS             complement(<1..909)
                     /locus_tag="VFJ67_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HET7290551.1"
                     /translation="MGYSKELLEQLNYGEGMTLYGDTPLAILKAFLQSGVSYLGGYPG
                     SPTAGLIDVISDAYEPVLKNKGIYFDCSGNEASAASLLSASISYPIRGSVNWKIVGTN
                     VASDALSHISSSGVTGGAMIIVGEDYGCDSTCVAERALPFALKSTMAVIDPRGDLDQV
                     AKMVEVGFELSEYSNMPVMYLLSTRVAHIMNKMTCRDNREAKISTINRLDDLITDLEK
                     IPLPPFTFDHEKKKYHERIPAARRFILERRLNEFFEGTETSMGIITHGMIYNSLMRCL
                     YNLGEASLYGECRIPILNLNVTNPLVP"
     gene            complement(1016..1486)
                     /locus_tag="VFJ67_08960"
     CDS             complement(1016..1486)
                     /locus_tag="VFJ67_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HET7290552.1"
                     /translation="MPERFSWVVEGVIAGMERPGLLGSREDDLEFLRGEGIDVIVNLE
                     EKEHARDYEGFIVKHIPVNDFKAPGLGDFEEFIEFVNDQLARNRRIAVHCYAGMGRTN
                     LMLACFLIHHMRIDPGTALEVVSMRRPFHLVTWQQEEALRDYFFMIRDSLVLRE"
     gene            complement(1479..2189)
                     /gene="nfi"
                     /locus_tag="VFJ67_08965"
     CDS             complement(1479..2189)
                     /gene="nfi"
                     /locus_tag="VFJ67_08965"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HET7290553.1"
                     /translation="MAIIDSDTLEELYRGDPPSPARAFEIQRELSGKVIRESEIGEPE
                     TVAGADLSVMKSVNKLVCGIILFSYPDLAEIERVDTVSDERFPYIPGLLAFREGPAII
                     EAYGKLKRKPDLLIIDGQGIAHPRGFGIACHVGVLLGIPTLGIAKKKLYGTYVEPAEK
                     KGSWSPLLSKEGDIIGSVLRTRDNTKPVFVSPGHKLDTESARKIALECARGYRIPEPT
                     RRADIYVAELKKEVSGLA"
     gene            complement(2176..2427)
                     /locus_tag="VFJ67_08970"
     CDS             complement(2176..2427)
                     /locus_tag="VFJ67_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290554.1"
                     /translation="MEKQAPTPEEIAEENRRLRLLRFMVDFTISLMYQTNLTREEALD
                     HFEKVRDFALRLFPGKELAFELIYAPRFKRVIRDIYGYH"
     gene            2600..3550
                     /gene="gshB"
                     /locus_tag="VFJ67_08975"
     CDS             2600..3550
                     /gene="gshB"
                     /locus_tag="VFJ67_08975"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET7290555.1"
                     /translation="MKLRMAFVMDPVGAINTEKDTTYVLMLEAQARGHEVYYLELKDL
                     YVKNARAFGNASRISLKRGRDYYKLGERETAGMDTFDVVWMRKDPPFNQDYIYATYIL
                     SLIDQSKTKVINDPRGIRESNEKLYTLYFPEWIPGSIVTKDIEQLNKFLAEEGGEIVV
                     KPLDGHGGEGVFYVREGDRNSNVILETITNFGKQYVLAQRFIKEVSEGDKRIILLNGE
                     PLGAVLRVPKPGGEFRCNFHSGGSPAKSDLTERDIKICKALSRRLKKDGLYFVGIDVI
                     GGYITEINTTSPTGIQEINRLNGVKLEADVIDFVEGLCSH"
     gene            3603..3893
                     /gene="gatC"
                     /locus_tag="VFJ67_08980"
     CDS             3603..3893
                     /gene="gatC"
                     /locus_tag="VFJ67_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET7290556.1"
                     /translation="MKITREDVIKVAELARLEFREEELEKFTEQLGNILTYIGKLNEL
                     DTKDVEPTSHVLDLATPLREDKVVEWLTREEALENAPREEDGFFVVPQVIED"
     gene            3906..5366
                     /gene="gatA"
                     /locus_tag="VFJ67_08985"
     CDS             3906..5366
                     /gene="gatA"
                     /locus_tag="VFJ67_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET7290557.1"
                     /translation="MELPFLSIGEASGLIKKGEISPVELTESVLDRVSRLDGELNTYI
                     TVMGEKAIDAAKTAEKEISSGKYRGPLHGIPIGLKDIFVMKGVPATCGSKMLENFIPP
                     YDAAVTKKILDAGAVIVGKNNMDEFAMGSSTETSYFGPTKNPWDKGRVPGGSSGGSAA
                     ATAASLCLGSIGTDTGGSIRQPASFCGVVGMKPTYGRVSRFGMIAFASSLDQAGPITK
                     TVEDAAIILNVISGHDPMDSTCVNTQAPDYTLSLRDDIKGVKVGVPREYFIPGMDKEV
                     EEAAKKAIALVEDLGGEIVEISLPHTEYAVITYYIIAPSEASSNLARYDGVKYGYRTA
                     NAETLRDMYFKTRAEGFGSEVKRRIMLGTYALSAGYYDAYYLKAQRVRTLIKKDFDDA
                     FKKVDVIMAPTAPEVAFRFGEKTGDPIKMYLSDVLTIPCNIAGLPGISVPCGFSSDGL
                     PIGIQVLGKPFDECSVIHVAHAYEQRAGWRDRKPDI"
BASE COUNT         1337 a         1472 c         1566 g         1162 t
ORIGIN      
        1 gggcacgagc gggttagtaa cgttcaggtt gagtatcggt atcctgcact cgccgtaaag
       61 gctcgcttcg cccaggttat atagacacct catcagcgag ttatatatca tcccgtgtgt
      121 gattatcccc atgctggttt cggtcccttc gaagaactcg ttgagcctcc tctcgagtat
      181 gaacctcctc gcggcgggta tccgttcgtg gtatttcttt ttttcgtggt cgaaagtaaa
      241 aggaggaagt ggaatctttt cgagatcggt tatcaggtcg tcgagccggt tgatcgtgct
      301 tattttagcc tccctgttgt cccggcaggt catcttgttc atgatgtggg ctaccctcgt
      361 ggaaagcagg tacatgacag gcatgttgga gtattccgaa agctcgaaac ccacctcgac
      421 catctttgcg acctgatcga ggtctcccct cggatcgatc actgccatcg ttgacttgag
      481 agcgaacggg agcgccctct cggcgacgca cgtgctgtcg cagccatagt cctccccgac
      541 gatgatcatg gcccctccgg tgaccccgga ggaggatatg tgagaaaggg cgtcggaggc
      601 gacgttcgtg cctactattt tccagtttac cgacccgcgt atcgggtagc ttatagatgc
      661 tgacagcagc gacgcggcag acgcctcgtt gcctgagcag tcgaaatata ttcctttatt
      721 tttgagtaca ggctcgtatg cgtcagagat gacatcgatg agcccggccg tcggagagcc
      781 cggataaccg ccgagatagc tcacccccga ctggaggaaa gctttgagta tagcaagcgg
      841 ggtgtctccg taaagtgtca tcccttcgcc gtaattcaac tgttcgagca gttcttttga
      901 gtaacccatt acgtctcccg ctccgaagcc ttttcaacac cccggcgttt ataatctacc
      961 agatatgagg cgtatatacc agattaacta ctttgcacag cacggtctaa ctcgtctatt
     1021 ccctgagcac taaactgtcc cttatcatga agaaataatc ccttagagcc tcttcctgct
     1081 gccaggtgac cagatgaaag ggcctcctca tacttacgac ctcgagagcc gtgccgggat
     1141 cgatcctcat gtggtgtata aggaagcatg cgagcatgag attggtcctg cccatccccg
     1201 cataacagtg gaccgcgatg cgcctgttgc gggcgagctg atcgttgacg aattcgatga
     1261 attcttcgaa atccccgagc ccgggcgcct tgaaatcgtt tacgggtatg tgctttacta
     1321 tgaagccctc gtaatccctc gcatgctcct tttcctcgag gttaacgatc acatcgatac
     1381 cctcccctct caggaattcg aggtcatcct cgcggctgcc gaggagcccc ggcctttcca
     1441 tgcccgcaat aacaccttcg acgacccacg agaacctttc aggcaaggcc ggacacctcc
     1501 ttcttgagct cggcgacata gatatcggcg cgtctcgtcg gctcgggtat tctgtacccc
     1561 ctagcgcact caagagcgat cttcctcgcc gattccgtat ccagcttgtg tccgggggag
     1621 acgaaaacgg gcttcgtatt gtccctcgtc ctcaggacag agccgataat gtccccttcc
     1681 ttcgacagta acggactcca tgagcccttt ttctcagccg gctcaacgta tgtgccgtag
     1741 agcttcttct tcgctatacc cagggtcggg atgccgagaa gcactccgac gtggcacgct
     1801 ataccgaacc ccctcgggtg cgctatcccc tgaccgtcta ttataaggag gtcgggcttc
     1861 cgcttaagct tcccgtatgc ctcgatgatc gcaggccctt ccctgaaagc gaggagcccc
     1921 ggaatgtaag ggaacctctc atccgaaacc gtgtcaactc gctctatttc ggcgaggtcc
     1981 gggtaagaaa ataatattat cccgcatacg agcttgttca cgctcttcat gacggaaagg
     2041 tcggctcccg caacagtctc aggctcgccg atctcgcttt ctctaatgac ttttccggat
     2101 aattccctct gaatttcgaa cgcacgggcg ggcgagggag gatctcccct gtagagctct
     2161 tcgagtgtgt ccgagtcaat gatagccata gatgtcccgt atcaccctct tgaatctcgg
     2221 ggcgtatata agctcgaagg cgagctcctt ccccggaaag agcctgagcg cgaaatccct
     2281 gaccttctca aaatgatcga gcgcctcttc cctcgtcaga ttggtctggt acatgaggga
     2341 tatggtgaaa tctaccatga accttagcag tctcagcctt ctgttctctt ccgctatttc
     2401 ttcgggtgtg ggagcttgtt tttccatggc agtcggggcc cctggacgat ggtatttcag
     2461 atatatgtca ttaaagtata cacgaagcat ggcgtgggat taacattttt cccgctcccg
     2521 caaatgccgc gcgtatttgt ccggggccgg aaaattcggt agaatcaaac ggcatcaaca
     2581 aggccttcag gaaacaggaa tgaagctcag aatggcattc gtaatggacc cggtcggggc
     2641 aataaatacc gagaaggaca cgacctacgt cctcatgctg gaggcccagg caagggggca
     2701 cgaagtctac tacctcgagc taaaagacct gtacgttaaa aacgcgcgtg ccttcggaaa
     2761 cgcctcccgt atcagcctca agaggggcag agactattat aagctagggg aaagggagac
     2821 ggccgggatg gacacgttcg acgtcgtctg gatgaggaaa gacccgccgt tcaaccagga
     2881 ctacatatac gcgacttaca tactgagcct gatagaccag agcaagacga aggtgataaa
     2941 cgacccgcgc ggaataaggg agtctaacga gaagctctat acgctctatt tccccgaatg
     3001 gataccgggc tcgatcgtga ccaaggacat cgagcagctg aacaagttcc tcgccgagga
     3061 gggaggggag atcgtcgtga agccgctaga cggccacggg ggtgaagggg tcttctacgt
     3121 gcgcgaaggg gacaggaact caaacgtgat actcgagacg attacgaact tcgggaagca
     3181 gtacgtgctc gcgcagaggt tcataaagga agtgagcgag ggggacaaga ggataatact
     3241 cttaaacggc gagccgctcg gagccgtgct cagggtgcca aagccgggag gggagttcag
     3301 gtgcaacttc cactcgggag ggagccccgc aaaatcggac ctcacggaga gggacatcaa
     3361 aatttgcaag gcgctctccc ggagactcaa gaaagacggc ctgtatttcg tgggcataga
     3421 cgttatcggc ggttatataa cggagataaa cacgacgagt cccacgggca ttcaggagat
     3481 aaacaggctg aacggcgtaa aactcgaagc ggacgtaatc gatttcgtcg agggactatg
     3541 ctcgcattga ggccgggatt attcgcgctt aaacaacagt aacattcagc cgggagactt
     3601 aaatgaagat cacgagagaa gacgtaataa aggtcgcgga gcttgcgagg ctcgagttca
     3661 gggaagagga attggagaaa ttcacagagc agctcgggaa catactcacc tacatcggca
     3721 agctgaacga gctcgatacg aaagacgtcg agcctacgtc ccacgtgctc gatctggcga
     3781 caccgctcag ggaagacaaa gtcgtagaat ggctcacccg cgaagaagca cttgaaaacg
     3841 cgccccggga agaggacggc ttcttcgtcg tcccgcaggt aattgaggac taaggagata
     3901 cttgaatgga actccctttt ttatcgatag gggaagcatc cgggcttatt aagaaagggg
     3961 aaatctcccc cgtagagctt acggaatccg tactcgacag ggtatctagg ctcgacggag
     4021 agctcaatac ctacatcacg gtcatggggg aaaaggcgat agacgctgca aaaactgcgg
     4081 agaaggagat atcatcgggc aaataccgag gcccccttca cggcataccc ataggcttga
     4141 aagacatatt tgtcatgaag ggagtccccg ctacgtgcgg atcgaagatg ctcgagaact
     4201 ttatcccgcc ctacgacgcg gccgtgacga aaaagatatt ggacgcgggg gcggtcatcg
     4261 tggggaagaa caacatggac gagttcgcta tgggctcgtc gactgaaacc tcttatttcg
     4321 gtccgaccaa gaacccgtgg gataaaggca gggtgccggg gggctcgagc ggcggctcag
     4381 ccgcggccac ggccgcttcg ctctgtctgg gctctatagg aacggacacg gggggctcga
     4441 tacggcagcc cgcatccttt tgcggcgtgg taggcatgaa gccaacctac gggcgcgtga
     4501 gcaggttcgg catgatagct ttcgcctcgt cgctcgacca ggcgggaccg ataacgaaga
     4561 cagtcgagga cgcggccata atcctgaacg tcatatcggg gcacgaccct atggactcaa
     4621 cgtgcgtcaa tacccaggcg ccggattata ccctcagcct gagagacgat ataaagggag
     4681 tgaaggtggg agtgccgagg gagtatttca ttccggggat ggacaaagag gtggaagagg
     4741 cagcgaagaa ggcgatcgcg ctcgtcgagg acctgggagg ggagatcgtc gagatatctc
     4801 tcccgcatac ggaatatgcc gtcatcactt actacatcat cgctccgtcc gaggcgagct
     4861 ctaaccttgc ccgttacgac ggggtcaaat acggatacag gacggctaac gcggaaacgc
     4921 tccgcgacat gtacttcaag acgcgagccg aagggttcgg gagcgaggtc aaaagaagga
     4981 taatgctcgg gacctacgcg ctttcggccg gatactacga cgcctattac ctaaaggcgc
     5041 agagggtgag gacgctcatc aagaaagatt tcgacgatgc cttcaagaag gtggacgtaa
     5101 taatggctcc aaccgcgccc gaggtcgcgt tcaggttcgg cgaaaaaacg ggcgacccga
     5161 taaagatgta cctgtcggac gtccttacga taccgtgcaa catcgccggg ctccccggga
     5221 tatccgtccc ctgcgggttc tcgtcggacg ggctgccgat tgggatacag gtgctcggca
     5281 agcctttcga cgaatgctcc gtcatacatg tcgcgcacgc ttacgaacag cgcgccggct
     5341 ggagggacag gaagccggat atttaggaag cccgcgagcc gaatgaagat atttcgcccg
     5401 ggaattgcac gatggggtag aattaagcaa gggagacatt cacgatgacc gacaagaaaa
     5461 aggtatgcat gatatgcggg aaaccgtccg aaacatcgat ctgcgatgag tgcaaggcga
     5521 agatccaggg ggaggct
//
LOCUS       DASSJH010000409         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_531063, whole genome shotgun sequence.
ACCESSION   DASSJH010000409 DASSJH010000000
VERSION     DASSJH010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531063"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="VFJ67_08990"
     CDS             complement(<1..1478)
                     /locus_tag="VFJ67_08990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7290558.1"
                     /translation="MNPVSTETNNRETEIARITILPEKAVINALADMVGNIAQAYGIG
                     RKQSVNLDRVLLDIIDNIVESGFEGDRRKPVDVVVSKRLHTLVVAVEDKGLPFDYERI
                     ERGEEKRFSAYLSRHYADEVNFSTLGTGGNRTEIVKNLPATDIRTVMDISEHTEHAGA
                     AQAPPGEKITIGMLDTGKVHELVRLVYKCYGYTYANEFMYYPEQIEARLTSGVMLSGA
                     AYNSREEIIGHVGFIFSAPGARVAESGEAVVDPRYRGRGIFEKIKNHLVEVVTPMNVA
                     GIYGEAVTVHPYSQKGSIELGGRETGFLLGYSPGTVTFHNISEEERPRRQSVAMMFTP
                     VSRSGRETVYIPQVYKDMIGGIFERIGFDRKLIVENEGTGFKGRQKGSTTVSVRHDHN
                     QALIKLEKVGTMTVKEIGFQLKHLARERFDCIYVDIPLRQKGAGYAASAVRRLGFFFG
                     CIIPEYGDGDVLRMQYLNNVEISRDDIKTASEFGQDLLDYIFA"
     gene            complement(1503..>2462)
                     /locus_tag="VFJ67_08995"
     CDS             complement(1503..>2462)
                     /locus_tag="VFJ67_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7290559.1"
                     /translation="GIPASGNLLDSTSALDLPDIPKSMLVVGGGYIGLELGTVYAALG
                     TEVSVVEMTGGLMPGVDRDLVRVLQKRSEQIYKSIMLETKVVGIKEIGNGILVTLEGK
                     GKEKKELVYDKVLVSVGRKPNSEGLGLENTGVETDGKGFIRVDEERRTAEPNIFAIGD
                     VAGEPMLAHKATHEGRVAAEVIAGMKSAFMPNAIPAVVFTDPEIAWCGITETEAKERN
                     IPVSAARFPWGASGRATTLGRQDGVTKIITDPESGRVLGVGIVGPGAGELIAEGVLAV
                     EMGALASDIALSIHPHPTLSETLMEAAEALEGSSTHIYRPGKK"
BASE COUNT          420 a          827 c          591 g          624 t
ORIGIN      
        1 gcgaatatat aatccagaag gtcctgtccg aattcagaag ccgtcttgat gtcgtccctc
       61 gatatctcga cgttgttcag gtactgcatt ctcagcacat ccccgtcccc gtactccggg
      121 atgatacaac cgaaaaagaa gccgagcctg cgcacggcgg aggccgcata gcccgccccc
      181 ttctgcctta gcggaatatc gacgtatatg cagtcgaacc tttcgcgcgc gaggtgcttc
      241 agctgaaacc cgatctcctt tactgtcatc gtccccacct tctctaactt tatcagcgct
      301 tggttgtggt cgtgtctgac acttacggta gtgctaccct tctgcctacc tttaaaaccc
      361 gtcccctcgt tctctacgat caattttctg tcgaacccta tacgctcgaa tattccgcct
      421 atcatgtcct tgtaaacttg agggatatag accgtctcgc gcccgctcct cgagacaggt
      481 gtgaacatca tggcgaccga ctgccgcctc ggcctttcct cttccgatat attgtggaac
      541 gtgaccgtgc cggggctgta gccgaggaga aatcccgtct ccctgccgcc taactctatg
      601 ctccccttct ggctgtaggg atgcaccgtg accgcctcac catatattcc ggcgacgttc
      661 atcggggtga ctacttcgac gagatgattc tttattttct cgaatatgcc cctccccctg
      721 taccgcgggt ctaccaccgc ttccccggac tccgccaccc tcgctcctgg cgcgctgaat
      781 atgaatccca cgtgtcctat gatctcctcc ctgctgttgt acgccgctcc cgaaagcatc
      841 acccccgagg tgagccgcgc ttctatctgc tccgggtaat acatgaattc gttcgcgtac
      901 gtgtagccgt agcacttgta aacgagcctt acgagctcgt gcacctttcc cgtgtcgagc
      961 atcccgatag tgattttctc gccgggcggg gcctgggcag ctccggcgtg ttctgtatgc
     1021 tcggatatat ccatcacggt ccttatatct gtcgcaggca ggttcttcac gatctcggtc
     1081 ctgttccctc ccgttcccag cgtcgagaaa ttcacctcgt ccgcgtaatg cctggacagg
     1141 tatgcgctga acctcttctc ctccccccgc tcgatcctct cataatcgaa cggcagcccc
     1201 ttgtcctcta ccgcgactac gagcgtgtgt agacgcttcg atacgacgac gtcaacgggt
     1261 ttccgcctgt ccccttcgaa ccctgactcc acgatgttgt ctatgatgtc gaggagcacc
     1321 ctgtcgagat tcacggattg cttcctgccg attccataag cctgtgctat gttcccgacc
     1381 atatcggcga gcgcgtttat cacggccttt tccggaagta ttgtgatgcg ggctatctct
     1441 gtctctctgt tattcgtttc cgttgatacc ggattcatag tgttcttagc tcaggacgcc
     1501 cgttattttt tgccgggcct gtaaatgtgc gtgctcgatc cttcgagagc ctcggccgcc
     1561 tccatcagcg tctctgacag cgtcggatgc gggtgtatgc taagggcgat gtccgatgcg
     1621 agcgccccca tctccacggc aagcactcct tccgcgataa gctcgcccgc ccccggtccg
     1681 acgataccca cgccgagcac tcgtcccgat tccgggtccg ttattatctt cgtcacgccg
     1741 tcctgcctgc cgagagtggt cgcccttccg gacgctcccc aggggaacct cgcggcggat
     1801 accggtatat ttctttcctt ggcctctgtc tccgtaatcc cgcaccacgc tatctccggg
     1861 tccgtgaata cgactgcggg tatggcattg ggcatgaacg ccgacttcat acccgcgatg
     1921 acctctgccg ccaccctgcc ctcgtgtgtg gccttgtgag cgagcatggg ctcgccggcg
     1981 acgtccccta tcgcgaatat attgggctcg gcggtcctcc tctcctcgtc cacgcgtata
     2041 aaccccttac cgtccgtctc cactccggtg ttctcgagtc cgagcccttc gctgttgggc
     2101 ttcctgccca ctgaaaccag caccttatcg taaacgagct ccttcttttc cttccctttc
     2161 ccctcgagcg taacaaggat cccgttcccg atctccttta tgccgacgac cttcgtttcg
     2221 agcattatgg acttatagat ttgctccgac ctcttttgca gcacccgtac gaggtccctg
     2281 tcgacgccgg gcatgagccc tccggtcatc tccacgacgg acacctccgt gccgagcgcc
     2341 gcgtacacgg tcccgagctc gagccctatg tatccgcctc cgaccacgag catcgatttg
     2401 ggtatgtccg ggagatcgag ggccgacgtc gaatcgagca aattgccgga cgcaggaatt
     2461 cc
//
LOCUS       DASSJH010000410         7183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1608855, whole genome shotgun sequence.
ACCESSION   DASSJH010000410 DASSJH010000000
VERSION     DASSJH010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608855"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFJ67_09000"
     CDS             <1..880
                     /locus_tag="VFJ67_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7290560.1"
                     /translation="NDALLKMVNHYNVFAEVEPSQKERIINALKKAGNVAGYMGDGIN
                     DASALHAADVGISVESAVDVAKQAADIVLLKMDLGVLVNGVREGRVTFANTLKYVFMA
                     TSANFGNMFSMAGASLFLSFLPLLPKQILLTNLMTDLPEMTIAGDSVDPEMSEKPRRW
                     DIRFIRNFMATFGLVSSIFDYMTFGVLLLIFHATTDQFRTGWFIESVVSATLIILVIR
                     TRRPFLLSRPGKYLALAVFLVICGALYLPYSRLAGLFNLHPLPPSFLIALGVIVVLYV
                     ITAEAAKRIFYRKVRG"
     gene            complement(894..3554)
                     /locus_tag="VFJ67_09005"
     CDS             complement(894..3554)
                     /locus_tag="VFJ67_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7290561.1"
                     /translation="MNGTPGPTQKQQEILNAEGNVLVTANPGTGKTFLLSRKYLKVVR
                     DGIPPEEILCLTFTDKARREMEERIIKLLSESGIKPDLSKLNIYTFHSYALENLERGN
                     IVSSNLLRFTVYRYLADEEVFNYASSRLIDTIVPKIENLIRYLKSFGITPDKINLEKT
                     SSMLEGDKKISRKEKEMFAREFVKIFERYEEVKKGGGMDYADLLINFRMLDEIPRYEL
                     VLVDELQDVNRMEAEIAIRSGKRFFAVGDKKQAIFGFQGGSILNFGLFEDSSHFILSE
                     NWRSTDEILDYAREYFVSKTKDASHGRELAELKSAGGFSGGKPVIYQVERKLIPRAVA
                     EILKSPAGEKGSTAVIVRTNGQIMEISRELTKENIPFSSTFLTASNEARTSIIKYLRG
                     MLSNNIDEIKNALVTPFAPVPLQDAFDILDDRDVTLETLREKAPAFMAERDALNSTED
                     VNRLFTGRIIPAASPYGREYFTAAINLQDAFQEALGFIKDMNRESLLAYLRTADLTTD
                     EIETDSGITLTTVHKAKGKEYDTVIYAPFSPPDKSNYQDEVVEAILRLEGIDAEEELE
                     EESLRINFVAFTRAKRNLSVVTEKFSEFHNSHAEEGHIEVSAARDMDIDESRKKAFTC
                     FVSGRFEMARDLLERKESWLTDYVKNFFGSLGSISFSSVNNSAYEFLVNYILRIREDT
                     KALTTGLNVHAIADELIKKGECECPPDLEPFRENITKLLGTIKQDYPESHSTEMSFKV
                     PLGGIIDTDEEILFTGFIDAIFRNGDSYLIVDWKTDRNTDKGSEHRQQLEAYKRAFCL
                     ANGLDPDKVKISIGFVGLRSALNTGTIECALDEKQPAKTSFDTFAKRVQNVLRWKKDP
                     ESFFHEILEAEINDPLWRSIVEQYIRERKD"
     gene            3684..4607
                     /locus_tag="VFJ67_09010"
     CDS             3684..4607
                     /locus_tag="VFJ67_09010"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7290562.1"
                     /translation="MATMKINPGGTRIGWIGTGVMGASMCMHLVKAGYKTVVFNRTRK
                     KAEGLIRAGAVWAKSPEDAASKSDVLFTIVGFPGDVREVYFGKDGIFEGLRKGSVVVD
                     MTTTEPSLAVEIYSAAKKAGASAVDAPVSGGDVGAREGKLSIMAGGDRKTFDSLMPLF
                     GLLGKNIVYQGGPGFGQHTKMCNQIMAASLMIGMCETLLYAYKAGLDPETMLMSVGGG
                     AAACWMLDNLAPRVLRRDFSPGFYVEHFIKDMGIALMEAERMDLVLPGLSLVRQLYVA
                     AKAQGHGRKGTQALLLALESISGIERKEPKK"
     gene            4689..4916
                     /locus_tag="VFJ67_09015"
     CDS             4689..4916
                     /locus_tag="VFJ67_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290563.1"
                     /translation="MKEILLTLLLMVGPIWATSCATLDGPAEPASTDTLEDRTEPASM
                     DSKYNQEVEEEIESEEAPLATEEMEDQPLIK"
     gene            complement(4971..5753)
                     /locus_tag="VFJ67_09020"
     CDS             complement(4971..5753)
                     /locus_tag="VFJ67_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HET7290564.1"
                     /translation="MAAAALAISIYIFTAAPYRLYGTSGESAVPVFGYEIVKTYPHDV
                     DAFTQGLFIRSGMLYEGTGLNGRSSLKKVDLETGKVLEERELPNAYFGEGIATDGKRI
                     IQLTWKSRKGFVYDLKTLAPLSEFSYPTEGWGIAYDGRSFIMSDGSATLYFLDPWKFK
                     EIGRLEVYDDKGPVVRLNELEYVKGEIFANVWGEERVARIDPETGRVTAWIDLSGLLS
                     ESDRKNRVDVLNGIAYDDEKGKFYVTGKLWPKLFEIKIVPKK"
     gene            6023..6853
                     /locus_tag="VFJ67_09025"
     CDS             6023..6853
                     /locus_tag="VFJ67_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290565.1"
                     /translation="MHFSGAERLIGKVRIRTDAGFDTQRLLLPLAILVQLFALASESL
                     AVGGLSGSKLVVPEAGTVSKGHIEVEPFFSLEFVDDDNDTVRYGGGLRLTPGLLENLE
                     AGVNINYLDVEDSGLISAETDFGDIEAGFKLRFIDQKAGLPFSLAYQAGVTFPAGKGA
                     EWIVEPGGLILTKEFTEEFSMDADFVFGLIEHGSWSFVTDVGFGYYLNSWFQPVIEAA
                     YAYEDPEDEPGIWVLNVSAGFTADVSDWLTIVFGVTPDLHTENTEKEVVVTSAFTFTF
                     "
     gene            6936..>7183
                     /locus_tag="VFJ67_09030"
     CDS             6936..>7183
                     /locus_tag="VFJ67_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET7290566.1"
                     /translation="MYTESIENYLKAIYEIQKEKGKVSTNALSEKLRVAPASVTSMIK
                     KLSEKKLLTHKRYQGVKLTQAGQKIALEVVRHHRLIEL"
BASE COUNT         1483 a         1893 c         2039 g         1768 t
ORIGIN      
        1 caacgatgct cttctcaaaa tggtcaacca ctataacgtc ttcgccgagg tggagccgag
       61 ccagaaggag cgcatcataa atgctttgaa gaaagccggg aacgtcgccg gctacatggg
      121 ggatggtata aacgacgcat cggcgctcca cgcggcagac gtcggcatat cggtcgagag
      181 cgccgtggac gtcgcgaaac aggcagccga catagtgctc ctcaagatgg acctcggcgt
      241 gctcgtgaac ggggtgaggg aagggagggt tacatttgcc aacacactca aatacgtttt
      301 catggcgacg agcgcaaatt tcgggaacat gttcagcatg gccggggcgt cgctgttcct
      361 gtcattcctg ccgctactcc cgaagcagat actgctaacc aatctgatga cggacctccc
      421 ggagatgacc atagcgggcg acagcgtcga cccggagatg tcggagaagc cgagacgctg
      481 ggacatacgt ttcataagga acttcatggc gacgttcggg ctcgtaagct caattttcga
      541 ttacatgacg ttcggcgtgc tgctgttgat cttccacgcg acgacggatc agttcaggac
      601 ggggtggttc atcgagtcgg tcgtatcagc gacgcttata atactggtca taaggacgag
      661 gaggccgttc ctcctgagca ggccggggaa atacctcgcg ctcgcggtct ttctcgttat
      721 atgcggggcg ctctacctgc cttattcccg gctcgcgggt ctattcaatt tacacccgct
      781 ccccccttct ttcctcatcg ctctcggggt gatagtggtc ctgtacgtga tcaccgccga
      841 agccgcgaag aggatttttt ataggaaggt gagggggtga tgttaaatag ccttcagtcc
      901 ttcctctccc taatgtactg ctcgacgatg ctccgccaga gggggtcgtt gatctcggct
      961 tcgagtattt cgtggaagaa cgattccggg tcttttttcc acctgagcac gttctgcact
     1021 ctcttggcga atgtgtcgaa tgaggttttc gccggctgct tctcgtcgag agcgcactct
     1081 atggtcccgg tgttcagcgc cgatctcagg ccgacgaagc cgatagatat cttgaccttg
     1141 tccggatcga gaccgttcgc gagacagaac gccctcttgt aagcctctag ctgctgtctg
     1201 tgctcggagc ctttatccgt attcctgtcc gtcttccagt cgactatcag gtacgagtcc
     1261 ccgtttctga atatcgcgtc tataaaaccg gtgaacagta tctcctcgtc cgtatctata
     1321 atgcctccga gagggacctt gaagctcatc tcggtcgagt ggctctcggg gtaatcctgc
     1381 ttaatagttc cgaggagttt cgtgatgttc tctctgaacg gctccaggtc gggcgggcat
     1441 tcgcattcgc ccttctttat gagctcgtcg gcaatggcgt gaacgttcaa tccggtcgtc
     1501 agggctttcg tatcctccct tatcctgagt atgtaattga ccaggaactc gtaggcgctg
     1561 ttattgacgg atgagaatga tatgctcccg agtgagccga agaaattttt cacgtagtcg
     1621 gtgagccagc tctcttttct ttcgagaagg tccctcgcca tctcgaaccg gccgcttacg
     1681 aagcacgtga acgctttctt gcgtgactcg tcgatatcca tatccctcgc ggcgctcacc
     1741 tcgatgtggc cttcctcggc gtgactgtta tggaattcgg agaatttctc ggtcaccacg
     1801 gagagattcc ttttcgcgcg cgtgaacgcg acgaagttaa ttcggagact ctcttcttcg
     1861 agctcttcct cggcgtctat gccttcgagc ctgagtatcg cttcgaccac ctcgtcctga
     1921 tagttcgatt tgtcgggcgg cgagaaaggg gcgtatatga cggtatcgta ctccttcccc
     1981 ttcgccttat ggaccgtcgt gagcgtgatg ccgctatcgg tctcgatctc gtccgtagtc
     2041 agatcggccg ttcggaggta tgcgaggagg ctctcgcggt tcatgtcctt tatgaacccg
     2101 agcgcttcct gaaaagcgtc ctgtaaattt atagcagccg tgaagtattc ccggccgtaa
     2161 ggggacgcgg cggggattat cctcccggta aagagcctgt tcacgtcctc tgtcgaattg
     2221 agcgcgtccc tctccgccat gaaagcgggg gctttctcac ggagcgtctc cagagtgacg
     2281 tcgcggtcgt ccaatatgtc gaatgcgtcc tggagcggta cgggggcgaa gggcgtaacg
     2341 agcgcgtttt taatctcatc tatgttattg ctgagcatcc ctctcaggta tttgatgatg
     2401 ctcgtcctcg cttcgttaga cgccgtgagg aaggtcgagg agaaaggtat gttctccttc
     2461 gtgagctccc tcgatatctc catgatctgc ccgttcgtcc ttacgattac cgctgtgctg
     2521 cccttttccc ccgccgggga tttcagtatc tctgctaccg cgcggggaat gagctttctc
     2581 tcgacctgat atatgacggg cttccctccg ctgaatcctc ccgcgctttt tagttcggcg
     2641 agctccctgc cgtgcgacgc gtctttcgtt ttcgatacga aatactctct cgcgtaatcg
     2701 agtatctcgt cggtgctcct ccagttctcg gacaatatga aatgagatga gtcctcgaac
     2761 aggccgaagt tgagtatgga gccgccctgg aacccgaata tcgcctgctt cttgtcgcct
     2821 acggcgaaga accgcttccc tgacctgatc gcgatctccg cctccatcct gttcacgtcc
     2881 tggagctcgt ccacgagcac gagctcgtag cggggtatct cgtcaagcat ccggaaattt
     2941 atgaggaggt ctgcgtaatc cattcctccg ccttttttca cctcttcgta ccgctcgaag
     3001 atcttcacga actccctggc gaacatctcc ttctctttcc tgcttatctt tttatcccct
     3061 tcgagcatgg atgatgtttt ttcgaggttt atcttgtcgg gcgttatgcc gaacgacttt
     3121 aggtaccgga tcaggttctc gatcttgggc actatggtgt cgatgaggcg ggaggaggcg
     3181 taattgaaga cttcctcgtc ggcgagatac ctgtagaccg tgaacctgag taggttcgat
     3241 gagacgatgt tgccccgctc aaggttctcg agcgcatacg aatggaacgt gtagatgttg
     3301 agtttcgaca ggtcgggctt tatcccgctc tcggaaagga gctttatgat gcgctcctcc
     3361 atttctctcc gggctttatc cgtgaaggtg aggcagagta tctcctcggg aggtattccg
     3421 tccctgacca ccttaaggta tttgcgcgag aggaggaatg tttttccagt gccagggttg
     3481 gccgtgacga gcacgttgcc ttccgcgttc agtatctcct gctgcttctg tgtaggtccc
     3541 ggggttccgt tcattggttc tggtcctgtc gcgcccggat ttagtttctc atacgcatgg
     3601 cataaaaaca attccgggtc ccttcatact ttacactgtc catctgaatt gatattatcc
     3661 atctttaaaa ggaggcgagg agtatggcga cgatgaagat aaaccccggt gggacgagaa
     3721 tagggtggat agggacgggc gtcatgggcg cgtccatgtg catgcatctc gtgaaagccg
     3781 gatataagac ggtggttttt aacaggacga gaaaaaaggc cgaggggctg atcagagcag
     3841 gggctgtgtg ggcgaaatcg cctgaagacg cggcctcgaa gtcggacgta cttttcacta
     3901 tcgtcgggtt ccccggcgac gtgagagagg tctatttcgg gaaagacggc atattcgaag
     3961 ggctccgtaa ggggagcgtc gttgtggaca tgaccacgac cgagccttcg cttgccgtgg
     4021 agatatattc cgccgctaaa aaggccgggg cttcggccgt tgacgcgccg gtttcgggag
     4081 gggacgtcgg ggcgagggag gggaagctct ccatcatggc cgggggggat aggaagacgt
     4141 tcgattctct catgcctctg ttcgggctcc tggggaagaa catcgtttat cagggagggc
     4201 cggggttcgg acagcacacg aagatgtgca accagataat ggcggcctcg ctgatgatag
     4261 gcatgtgcga gacgcttctc tacgcgtaca aagcggggct cgacccggag actatgctga
     4321 tgtccgtggg cgggggcgcg gccgcgtgct ggatgctcga caacctcgcg ccgagggtgc
     4381 tgaggaggga tttcagccct ggattctatg tcgagcactt cataaaggac atgggcatag
     4441 cgctcatgga ggccgagagg atggacctcg tcctgccggg gctttcgctc gtgaggcagc
     4501 tctacgtggc ggcgaaggcg caggggcacg gacgcaaggg cacgcaggcg ctacttctcg
     4561 cgctcgagag catatcgggc atcgagagaa aggaaccgaa aaaatagagg acccgatatt
     4621 tccgattggt ggtatattat ccggtgaatc ccgtacggat taaactcacg agtgaggtca
     4681 aaacgaatat gaaagaaatt ctcttaacgc tcctgttgat ggtcggccct atatgggcca
     4741 cgtcctgcgc gacgctcgac gggcccgccg agcccgcctc gacggatacg ctcgaggaca
     4801 ggaccgagcc cgcatccatg gatagtaagt acaaccagga agtagaggaa gagatcgaat
     4861 ccgaggaggc cccgctcgca accgaggaga tggaagacca gccgctcatc aagtaagatc
     4921 acggcacccg catacgcaga agaggacggc tatcacccgc accggggaag ttatttcttc
     4981 ggcactattt ttatctcgaa cagtttcggc cacagctttc cggttacgta aaactttcct
     5041 ttctcgtcgt cgtaagctat gccgttcagc acgtcgaccc tgttcttcct gtcggattcg
     5101 cttaataatc cgctcaggtc tatccacgcc gtaacgcggc cggtttcggg gtcgatcctg
     5161 gcgacgcgct cttcccccca gacgttcgcg aatatctcgc ccttgacgta ttcgagctcg
     5221 ttcaaccgca cgacagggcc cttgtcgtca tagacctcga gcctgccgat ctccttgaac
     5281 ttccacgggt cgaggaagta tagcgtcgcg ctcccgtcgc tcattatgaa gctccttccg
     5341 tcatatgcga ttccccaccc ttcggtcgga taactgaatt ccgaaagagg ggcgagcgtc
     5401 ttcaggtcgt aaacgaagcc tttacgggat ttccatgtga gctggattat cctcttgccg
     5461 tccgtagcga tcccttcccc gaaatatgcg ttggggagct cccgctcttc gagcaccttg
     5521 cctgtttcga gatcgacctt cttcaaggag gacctgccgt tcaagcccgt tccttcatac
     5581 agcatgccgc tgcgtatgaa gaggccctgg gtgaacgcgt ctacgtcgtg agggtacgtc
     5641 ttgacgattt cgtagccgaa taccgggact gcgctctctc cggaggttcc gtataaacgg
     5701 tatggggcag cagtgaagat atagatagat atggctagtg cggccgccgc cataaggata
     5761 agcgggatat gtgagcggaa tcccggttgg ggcttgcgtt tttcgtcagc ggaaagcatg
     5821 gcctatttgg atatatatta ccgcgttacg caggatttct cgcgtttaat cgtaatcgtt
     5881 tgactttata tgcgggatca tataatttag tctagactaa aattattctt agtgcattat
     5941 aaagaacatt tttgcaacag tgaagagatg ccgctttcgt aaaggggagg ttgcgatggc
     6001 gtttacccgt aaatcaatat atatgcactt ctccggagcg gagaggctca tagggaaggt
     6061 acggatacgg acggacgccg ggttcgatac acaacgtctt cttttgcccc tggcgatact
     6121 cgttcaacta ttcgctcttg cgtccgagtc tctggccgtc ggcggcctct cgggctccaa
     6181 gctcgttgtg ccggaggcgg gtactgtgtc gaaagggcat atcgaagtgg agcctttctt
     6241 ctcgctcgaa ttcgtcgatg acgataatga cacggtgagg tacggagggg gtttgagact
     6301 gaccccgggc ctgctggaaa acctcgaagc gggggtgaat attaattacc tcgacgtcga
     6361 ggactccggg ctgatatcgg ccgagaccga tttcggggat atagaggccg gtttcaagct
     6421 caggttcatc gatcagaaag ctggcctgcc tttctctctt gcctatcagg ccggggtaac
     6481 gtttccggcg ggaaagggtg ctgagtggat agtcgagccc ggcggcctga tcctgacaaa
     6541 agagttcacg gaagagttct cgatggacgc ggatttcgtt ttcgggctga tagaacacgg
     6601 ctcgtggagc ttcgtaacgg acgtgggctt cggatactac cttaactcgt ggtttcagcc
     6661 tgttatagaa gccgcgtatg cctatgaaga ccccgaagat gagcccggca tatgggtcct
     6721 caatgtctcg gccgggttca cggcggacgt ttccgactgg ctcacgatag ttttcggggt
     6781 gacgcccgac ctccataccg aaaatactga aaaagaggtc gtcgtcacgt cagcattcac
     6841 cttcacgttc taggcccgga tttgcttgac aatctcccga ctatggatta aatttatttt
     6901 tagacttatc taaaatcact tttaggagtt tataaatgta cacggaatcc atagagaact
     6961 atctgaaggc catctacgag atacagaaag aaaaggggaa ggtctcgact aacgcccttt
     7021 cggaaaagct acgcgtcgcg cccgcctccg tgacgagcat gataaagaag ctctccgaga
     7081 agaagctgct gacccataag aggtatcagg gggtcaagct gacccaggcc gggcagaaga
     7141 ttgcactcga ggtcgtaagg caccacaggc tgatagagct tta
//
LOCUS       DASSJH010000411         6062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1173982, whole genome shotgun sequence.
ACCESSION   DASSJH010000411 DASSJH010000000
VERSION     DASSJH010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173982"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="VFJ67_09035"
     CDS             <1..1498
                     /locus_tag="VFJ67_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HET7290567.1"
                     /translation="EKSDAGILPPFKRVVFDEAHHLTDAATSHFGMRATKYGIIRVLR
                     RMKRRGKGGEQKGLIYYSASLAARLVKVARKGIVNDVLRRVEQNLSPRTDAAEEYTAD
                     AFDALYNFSLSLMQEKEPESQEVSIRITESAFSREGWEEIDRKFSVLRIRLKELHEEI
                     KSFTDFLLDYEGENDFARLIVEFRGVANKLDYYSDVISTFLSPEDDGYVRWVEGKAGR
                     GAIVSGLGLSPLDISQQLKERLYSRCDTVVMTSATMAVRKEFDFIKSGLGLLDEPRVS
                     ELILPSPFDYEEQLLLSIPTDVPEPGTGEYSRVITPLILEAVKASRGNALILFTSYSL
                     LDNVYRQIHGELESSGILPLKQGALPRGRLLDRFRLEDGSVLFATDSFWEGVDVPGSA
                     LRLVVITRLPFRVPTEPIIEARVEHMESMGINSFTDYSVPVAVLKFKQGFGRLIRTRS
                     DRGVVLVLDRRIISKFYGRYFLDSLPECGRLMASSDEVISGLEEFFRE"
     gene            complement(1575..1718)
                     /locus_tag="VFJ67_09040"
     CDS             complement(1575..1718)
                     /locus_tag="VFJ67_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290568.1"
                     /translation="MRISKRITRALRISLIFMSLAVVFVVPGVMKCSGSVDVPEGKSS
                     AQI"
     gene            complement(2069..2842)
                     /locus_tag="VFJ67_09045"
     CDS             complement(2069..2842)
                     /locus_tag="VFJ67_09045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7290569.1"
                     /translation="MANETALITGASSGIGLELAKLFAADNSNLVLVARREERLRALA
                     DELKSEFGVDVFVLAKDLSQKSAPREIFDELRSQGIQVDVVVNSAGFGARGRFAELGE
                     EAQVDMIQVNVVALTHLTRLFLPGIIERGRGGILNVGSLAGFQPGPNLAVYYATKAYV
                     LSFTEALSEEVRNPDIKITCLAPGPVKTEFGEKSELDDSLLFKLSLMDAEPVVRAGYE
                     GFRKGKVIVLPGIKQKLIPFMLRFTPRFLVRKIVKSLQS"
     gene            complement(2899..3615)
                     /gene="mtgA"
                     /locus_tag="VFJ67_09050"
     CDS             complement(2899..3615)
                     /gene="mtgA"
                     /locus_tag="VFJ67_09050"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HET7290570.1"
                     /translation="MKRFLFGIIKFLIKTVLIVIAVTVLLVLLYRYVNPPLTPLMVIG
                     YFGEAKTIRKKWKDYNQISKYMKLAVIASEDQKFPLHDGFDIESIKEAIDEKMSGDRL
                     RGASTISQQTAKNVFLWPSRTWARKGAEAYFTFLIENIWSKKRILEVYLNVIETGKGI
                     YGVDEAGEIYFGKPAGKLSKEESALIAAILPSPAEMSAARPSRYVRERQSWIMGQMDN
                     LGTGYLKKIEQSRAKERTAD"
     gene            3778..>6062
                     /gene="gyrA"
                     /locus_tag="VFJ67_09055"
     CDS             3778..>6062
                     /gene="gyrA"
                     /locus_tag="VFJ67_09055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET7290571.1"
                     /translation="MPSQILPVNIEDEMKESYLSYSMSVIIGRALPDVRDGLKPVHRR
                     ILYGMEEAGNQWNRPYKKSARIVGDVMGKYHPHGDAAIYDALVRMAQDFSMRYTLVDG
                     QGNFGSIDGDPPAAMRYTEVRLDRIASEMLADLDKETVDFVPNYDGGEKEPLVLPAKI
                     PNLLLNGSSGIAVGMSTNIPPHNLGELIDAVVAQAGNPEITLDELMKHLPGPDFPTGG
                     FITGRAPIREAYETGRGIIRIRAKARIERQKKGDKEIIIVSEIPYQVNKVKLIERIAE
                     LVKDEKIKGISDIRDESDREGIRIVIEIKRGDNADVVLNQLYKHTQMDTSFGIIMLAL
                     VRNQPRVLSLKETITHFIEHRKEVVTRRTQYELGKAEERLHILEGLKIALDHLDEVIA
                     LIRKSASPQEAKEGLMLKLGLSDPQAQAILDMRLQRLTGLERDKILEERKELINTVKR
                     LKEILEHEHLILGIVTEELKEMREKYGDERRTEILESSEEISIEDLIADEDMVVTITH
                     DGYVKTTPLSVYRSQRRGGKGRTGITTKELDFVETLFIASKHNYVLFFTNLGRCYWIK
                     VHEIPEAGPTARGKALVNLLSLSDGEKVAAVLPVREFKESQYIIMATKNGIVKKTKLM
                     AYSNPRAGGIIALNIREDDELIEAKLTSGSDEILLTSYFGQAIRFAEDDVRDMGRTAT
                     GVIGIRLDEGDQVVSLEVIENPEAQVLTVTEMGYGKRTLVSEYRVTGRGGKGIITIKT
                     TGKNGRVVGAFQVTNDTQIMI"
BASE COUNT         1454 a         1626 c         1699 g         1283 t
ORIGIN      
        1 cgagaagagt gacgcgggca tcctgccccc gttcaaaagg gtcgttttcg acgaggctca
       61 ccatttaacg gacgcggcga cttcacactt cggaatgagg gcgaccaagt acgggataat
      121 cagggtcctc aggcgcatga agaggagagg gaagggcggg gagcagaagg ggctcatata
      181 ttacagcgcc tccctcgcgg caaggctcgt gaaggtcgcg agaaagggga tagtgaacga
      241 tgtcctgagg cgggtcgagc agaacctctc gccgaggacg gacgcagctg aggagtacac
      301 ggccgacgcg ttcgacgccc tgtacaactt ctcactatcg cttatgcagg aaaaggagcc
      361 ggaaagtcag gaagtgagca taaggattac ggagtcggcc ttctcacgag agggctggga
      421 agagatagac aggaaattct ccgtcctgag aataaggctg aaggagctcc acgaggaaat
      481 aaagtcgttt accgattttc tcctcgatta tgaaggggag aacgatttcg cgaggctgat
      541 agtcgaattc aggggcgtcg ccaacaagct cgactactat tctgacgtga tctcgacctt
      601 cctgagcccg gaggacgacg gatacgtcag atgggtcgag ggaaaggcgg gcaggggcgc
      661 gatcgtgtct gggctgggac tctcgcctct cgatatttcc cagcagttaa aagaaaggct
      721 ctactcgaga tgtgatacgg tcgtgatgac gtcggccacg atggctgtga ggaaggagtt
      781 cgatttcatc aagtccgggc tcggtctcct tgacgagcca agggtctccg agctcattct
      841 gccctcgccc ttcgattacg aagagcagct cctcctctcg ataccgaccg acgtacctga
      901 gcccggaacc ggagagtact cgcgcgtgat tacgccgctc atactggaag ccgtgaaggc
      961 atcccgggga aacgcgctca ttctattcac gtcgtattcc ctgctcgata acgtttacag
     1021 gcagattcac ggcgagctcg aatcctctgg catactcccg ctcaaacaag gcgcgctccc
     1081 gagagggagg ctcctcgaca ggttcaggct cgaggacggg tcggtcctgt tcgcgaccga
     1141 cagcttctgg gagggcgtcg atgtgccggg aagcgcgctc aggctcgtcg tgataacgag
     1201 gctccccttc agggtgccta ccgagcctat tatcgaagcc agggtcgagc acatggagag
     1261 catgggcata aactcgttca cggactacag cgtccccgtg gcggtactca aattcaagca
     1321 ggggttcgga aggcttatca ggactaggag cgacaggggc gtcgtcctcg tgctcgacag
     1381 gaggattata agtaaattct acggcagata ttttctggat tcccttcccg aatgcggccg
     1441 cctcatggct tcctctgacg aggtaatatc cgggctcgaa gaatttttca gggaataaag
     1501 accttgccgt cacacctcat gagcaaacac ggccctggct aacgggcact ccgtataact
     1561 tgacgatacc gaaatcaaat ttgagccgag gactttcctt cgggcacgtc caccgaaccc
     1621 gagcacttca ttactccggg cactacgaaa acgaccgcta ggctcatgaa gatgagtgat
     1681 atcctgaggg ctctcgttat tctcttcgaa attctcattt cagcaatcct cctttagtct
     1741 ctttacgctt tactcgttat cgaacgcggt gcttgtcaca gctgagcccg cggttttatc
     1801 ggctttggtc ccaagctcgc cctgacactg acagccgcag acgaaaagga gagcgaggag
     1861 gcaaaaggct tgtatcaccg cctttcgatt attcctgagt gatttaaaga tatacatgtg
     1921 tccgcccctt tactgactta tttaaacgcc gcctaaacga tacagattaa tacgaaatct
     1981 aatcatatca caccggaata acgagagcaa tcattaatta aatgtcagct attagttcat
     2041 gatctccggg ttctcaccgg ctcaggcgtc aggactggag cgacttcact atcttcctca
     2101 cgaggaatct gggcgtgaac ctgagcatga agggaatcag cttctgttta atgccgggaa
     2161 gcactataac cttccccttc ctgaacccct cgtaacccgc cctgacgacg ggctccgcgt
     2221 ccatgaggct cagcttgaac agaagcgagt cgtcgagctc cgatttctcg ccgaactccg
     2281 tcttcacagg accgggggcg aggcaggtta ttttaatgtc cgggttcctc acctcctccg
     2341 aaagcgcttc agtgaatgaa agcacgtacg ccttcgtcgc gtaatatacg gcgaggttgg
     2401 gccccggctg gaaccccgcg agagagccga cgttgagtat ccctccccgg cccctctcga
     2461 tgattcccgg caggaagagc ctcgtaaggt gcgtgagcgc gaccacgttc acctggatca
     2521 tgtctacctg cgcttcctct ccgagctcgg cgaacctccc gcgcgcgccg aacccggcac
     2581 tgttcacgac gacgtccacc tgtatgcctt gacttcgaag ctcgtcgaag atctccctcg
     2641 gggcgctttt ctgcgagagg tctttagcga gaacaaacac gtccactccg aattccgact
     2701 tgagctcgtc ggcgagggcc cggagccttt cttccctgcg cgcgacgaga accaggtttg
     2761 aattgtccgc cgcgaagagc ttcgcgagct cgaggccgat cccggaggaa gcccccgtta
     2821 taagcgcggt ctcgtttgcc attgtcttct ctctcctcaa ttgtgtatga attgttttac
     2881 atcttgtctt ccgcggtgtc aatcggcggt ccgctctttt gcccgggact gctcgatctt
     2941 cttcagatag cctgttccca ggttgtccat ctgccccatt atccagctct gacgctcgcg
     3001 tacatacctc gaaggtcttg cggcggacat ctctgccggg ctcggaagta tggcggctat
     3061 gagcgccgat tcctccttgc tgagcttccc ggccggtttc ccgaaatata tctcgcccgc
     3121 ttcgtccacc ccatatatgc ccttccctgt ctcaatcacg ttcagataca cctccaatat
     3181 cctcttttta ctccatatat tttcgataag gaaagtgaaa taagcttcgg ccccttttct
     3241 cgcccacgtc ctcgatggcc agaggaaaac gttcttggcc gtctgctggc ttatggtgct
     3301 cgcccctctc agcctgtctc cgctcatctt ttcgtcgatc gcctctttaa tggattcgat
     3361 gtcaaacccg tcgtgtagag ggaatttctg gtcttcggag gcgattacgg cgagcttcat
     3421 gtacttcgat atctgattat aatctttcca ctttttcctg attgtcttcg cctccccgaa
     3481 gtagccgatg accatgagcg gagtgagcgg cgggttcacg tacctataga ggagcaccaa
     3541 gaggaccgtg accgcgatta cgatcagcac ggttttaatc aggaatttaa taatgccgaa
     3601 gaggaatctt ttcatttaca gggcgattgc gggtctcccc ggtttttccg ggaatatcat
     3661 aggtttaacg taccttatcg actattccca gggcaaatca attgactctt gccgtcctct
     3721 catataacct tattagccaa tatttataga tatttaggag gcaagtgtta aatgacaatg
     3781 ccgtctcaga tactgcctgt caacatcgaa gacgagatga aggagtctta tctcagttat
     3841 tcgatgagcg taatcatcgg tagggccctt cccgacgtga gggacggtct caagcccgtg
     3901 cacaggcgga tactgtacgg gatggaggag gccgggaacc agtggaacag gccctataaa
     3961 aagtctgcca ggatcgtcgg agacgtgatg ggtaaatacc acccgcacgg cgacgccgcc
     4021 atatacgacg ctcttgtcag aatggctcag gacttttcca tgagatacac gctcgtcgac
     4081 ggtcagggca acttcggctc cattgacggc gaccctcctg cggcgatgag gtacacggag
     4141 gtgagactcg acaggatagc gagcgagatg ctcgccgacc tcgataaaga aacggtcgat
     4201 tttgtgccca actacgacgg cggggagaag gagcccctcg ttcttccggc gaagataccg
     4261 aacctgctat tgaacggctc ctccggtatc gccgtcggca tgtccacgaa cattcctcct
     4321 cataacctgg gcgagcttat agacgccgtt gtcgctcagg ccggcaaccc ggagatcacg
     4381 ctcgacgagc tcatgaagca ccttccgggg cctgatttcc ccacgggtgg tttcatcacg
     4441 ggccgtgcgc cgataaggga ggcttacgag acgggcaggg gaataatcag gatcagggcc
     4501 aaggccagga tcgagaggca gaagaaaggg gacaaggaaa taataattgt cagcgaaata
     4561 ccttatcagg tgaacaaggt caagctgata gagcgcatag cggagcttgt gaaggacgag
     4621 aagataaagg gcatatccga catcagggac gagtccgaca gagagggaat acggattgtg
     4681 atagagatca aacgcggcga caacgcggac gtagtgctca accagctcta caagcacacg
     4741 cagatggaca cctccttcgg cataatcatg ctcgctctcg tgaggaacca gccgcgcgtg
     4801 ctctcgctca aggaaacaat aactcacttc atagagcaca ggaaagaggt agtaaccagg
     4861 cgaacgcagt acgagctggg taaggcggaa gagaggctcc acatactgga agggctcaag
     4921 atagctctcg atcacctgga cgaggtcata gccctcatcc gcaagtcggc gagccctcag
     4981 gaagcgaagg aagggctgat gctgaaactg gggctttcag accctcaggc acaggcgatt
     5041 ctcgacatga ggctccagcg cctgaccggg ctcgagaggg acaagatact cgaggaacgg
     5101 aaggagctca taaacacggt caagcgtctg aaagagatac tcgagcacga gcacctgatt
     5161 ctcggcatag tgactgaaga gttaaaggaa atgagagaga agtacgggga cgagaggagg
     5221 acggagatac tcgagagctc ggaggagata tccatagagg acctcatcgc cgacgaggac
     5281 atggtcgtca ccataaccca cgacggctac gtcaagacca cccccttaag cgtttacagg
     5341 agccagagaa gggggggcaa ggggaggacc ggcataacta cgaaagagct cgatttcgtc
     5401 gagacgcttt tcattgcgtc caagcacaac tacgtcctct ttttcactaa cctcggcagg
     5461 tgttactgga taaaggtcca cgagataccg gaggcgggcc cgacggcgag agggaaagcg
     5521 ctcgtgaatc tgctgagctt atccgacggc gagaaagtcg cggcggtcct cccggttaga
     5581 gagttcaagg aaagtcagta catcatcatg gcgactaaga acggcatcgt aaagaaaacg
     5641 aagctcatgg cttattccaa cccgcgcgcg ggcggaatta tcgcgctcaa tataagggaa
     5701 gacgacgagc ttatcgaagc gaagctcacg agcggtagcg acgaaatact gcttacgtcc
     5761 tatttcggcc aggcgataag gttcgcggag gacgacgtga gggatatggg caggacggcg
     5821 acgggtgtta tcggtataag gctcgacgaa ggtgatcagg tcgtgagcct cgaagtcatt
     5881 gaaaaccccg aggcccaggt gctcactgtg accgaaatgg ggtacggaaa gaggaccctc
     5941 gtgtccgaat acagggttac gggacgcgga ggcaaaggca ttatcacgat caagaccaca
     6001 gggaagaacg ggagggtcgt gggggccttt caggtcacga acgatacgca gattatgata
     6061 at
//
LOCUS       DASSJH010000412         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1281339, whole genome shotgun sequence.
ACCESSION   DASSJH010000412 DASSJH010000000
VERSION     DASSJH010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281339"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VFJ67_09060"
     CDS             <1..1106
                     /locus_tag="VFJ67_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HET7290572.1"
                     /translation="RRSGKIKVRYVNPDKEPGIAKKYDIKEYGTVVLIDGQQTIKLRL
                     ADHISGGILKNAEEEITNALFKLNRVVHKSVYFLTGHGERDINDDLEPEGLGSLNAAL
                     RDEGYDVKEFLLLRGDPLPAKDSVLIVSAPKQALSLKEITDIKAFLDNGGKAIFLIEP
                     RSGGELVSILKSFGFAISDDIIVDPSSKLEGGGDIAPIVAQYAHHDITEGFRFATIFP
                     YSRSIDVIEKDGTDAEVIADTGEYSWSETNFELFDQGVAQQEEGDKKGPLGVAAVSED
                     GHNSRIAVFGSVDFVSNIFIDFSGNRDLFLNTVNWVAGDENLISIRPRAAKEGKLTIT
                     KKQTNVIFFFTVGLIPAVIFFSGMGIWWKRRRL"
     gene            1176..2114
                     /locus_tag="VFJ67_09065"
     CDS             1176..2114
                     /locus_tag="VFJ67_09065"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET7290573.1"
                     /translation="MSSYLKRFASTVIVFIIFVVLLASVLLYNREKKQPEKGSGKVFP
                     ALKTDDITSINLKSAGIEFVLRKEGGWVVESEGKVFKADESAVGDLVRDVGEMEVEKL
                     VSRDPANLDEYGFVDSLTEFSVHTKDTEYPVIVGDVSPVGSGIYIYDLGEGRVLIVKD
                     RYLWGFLRKKPEDFRERRLTRIEKDGVARITVRIGDFSTALVKDGGRWYEVIDGENRP
                     ADQKKVQELIETLSGMKAEGFEDDVHGNLEKYGLAEPVAEIVFYGKGSEEGVLFGKRK
                     DEDSYFAKAKGADSVYTVSKNYFIILPKNNEDYLSR"
BASE COUNT          561 a          500 c          644 g          429 t
ORIGIN      
        1 cgaggaggtc gggaaagata aaggtcaggt acgtcaatcc cgataaagaa ccaggtatcg
       61 cgaaaaagta cgatattaaa gaatacggca cagtcgtgct catagacggg cagcaaacta
      121 taaagctgag actggctgac cacatctcgg gagggatact caaaaacgcg gaggaggaaa
      181 tcactaacgc cctcttcaag ctcaaccgtg tggttcacaa gtccgtctat ttcctgaccg
      241 ggcacggcga gcgcgacatt aacgacgatc tcgagcccga gggcctcgga tccctcaatg
      301 ccgcgctcag ggacgaaggg tacgacgtca aggagttcct gctgctgcgc ggggacccgc
      361 tgcccgctaa agacagcgtc ctcatcgtgt ctgccccgaa gcaggcgctt tcgctcaagg
      421 aaatcacgga tataaaggca tttctcgata acggagggaa ggcgatattc cttatagagc
      481 ccaggtcggg gggcgagctc gtctctatcc ttaagagttt cggtttcgcg atctcggacg
      541 atatcatagt cgaccccagc tcgaagctcg aagggggagg agacatagcg cccatagtcg
      601 cccagtacgc tcaccatgat ataacggagg ggttcaggtt cgcgacgatc ttcccttact
      661 cgaggagcat agatgtaatc gagaaggacg gcaccgatgc cgaagtcatc gccgatacgg
      721 gcgaatacag ctggtccgaa accaacttcg agctgttcga tcagggcgtc gcacagcagg
      781 aagaagggga taagaaaggc ccgttagggg tggctgcggt aagcgaggac gggcataatt
      841 cgaggatagc ggtcttcgga agcgttgact tcgtttcgaa tatatttatc gatttttcgg
      901 gcaacaggga cctttttctc aatacggtca actgggtcgc cggggacgag aacctcatct
      961 ccatacgccc gagggccgcg aaagaaggca aattaacgat aaccaagaag cagacgaatg
     1021 tgatattctt ctttacggtc gggctgatac cagccgtcat atttttctcg ggtatgggga
     1081 tatggtggaa gagaagaagg ttgtgagata tattcctcct gagggagcgg ccataatatt
     1141 caagccccga gtgcgtaaac cgttgagcat gtaaattgag cagctacctg aaaagattcg
     1201 cgagcactgt aatcgtattc attatcttcg tagttctgct tgcatccgtc ctgctctata
     1261 atcgtgaaaa aaaacaaccg gagaagggtt cgggaaaggt cttcccggcc ttaaaaacgg
     1321 atgacataac ctcgatcaat ctcaaatccg cgggcatcga attcgtactc cggaaagagg
     1381 gcggctgggt ggtggaaagc gaggggaagg tatttaaggc ggacgaaagc gccgtcgggg
     1441 acctcgtccg tgacgtcggg gaaatggagg ttgagaagct cgtatcgcgt gaccccgcca
     1501 atctggatga gtacgggttt gtggactcgc tgacggaatt ctccgtacac acgaaggaca
     1561 cggagtatcc cgtaatcgtc ggcgacgtga gcccggtcgg ctcggggata tatatctacg
     1621 acctgggcga ggggagggta ctgatagtca aagaccggta cctctggggg ttcctcagga
     1681 aaaaaccgga ggacttcaga gagaggaggt tgacccggat tgaaaaagac ggcgtggcgc
     1741 gtatcacggt caggataggt gatttctcga ccgcgctcgt taaagatggc ggcaggtggt
     1801 acgaggtgat cgacggggaa aaccgcccgg ccgatcagaa gaaggtgcag gagctcatcg
     1861 aaacgctctc ggggatgaag gctgaaggat tcgaggacga cgtccacggg aatctggaga
     1921 agtacgggct cgccgagccc gtcgcggaga tcgtctttta cggtaagggg tctgaagagg
     1981 gcgtattgtt cggcaaaagg aaagacgaag acagttattt cgccaaggcc aaaggcgctg
     2041 actccgtcta cacggtctca aagaattatt tcattatcct gcccaaaaat aacgaggact
     2101 acctgagtag gtaacgcttt ggacaaatta cggt
//
LOCUS       DASSJH010000413         4310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1397017, whole genome shotgun sequence.
ACCESSION   DASSJH010000413 DASSJH010000000
VERSION     DASSJH010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397017"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFJ67_09070"
     CDS             <1..289
                     /locus_tag="VFJ67_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290574.1"
                     /translation="GISFSAEIPVVIAQGESKQSGNELLKAVQAGSDFKGMTKNDVID
                     SYGQPSSKDTTGEKGRYDEKWNYSCETHNGLDYDCVFIYFMADRVVDVETF"
     gene            306..854
                     /locus_tag="VFJ67_09075"
     CDS             306..854
                     /locus_tag="VFJ67_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET7290575.1"
                     /translation="MRKCPKCGKELESRVLGPIDVDECESCKGVWLDAGELGKAEEAA
                     DPNINWLDFEIWKHPEKFKSRASDLKCPSCLENTVAIEYGHTGVEINYCPSCRGIWLE
                     KDELPKIIESLEDEISKKTVSDYVHESIREAKEIVTGHESFMSEWKDLSSILRLMQYR
                     LFVEKPKLIATLTDINTLNPFK"
     gene            complement(913..1290)
                     /locus_tag="VFJ67_09080"
     CDS             complement(913..1290)
                     /locus_tag="VFJ67_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290576.1"
                     /translation="MKLNPSLDYTSDSNDGPLITGERFSDGSLSKGTPAYIIFYHREC
                     YNSKRQARRTVELYGKYKGKVDFIIVDLDTGLSKEQEALRSRFYRNYIPHVTIIGGDG
                     MVLYDKSGEVDTDKISGILDRAL"
     gene            1577..2200
                     /locus_tag="VFJ67_09085"
     CDS             1577..2200
                     /locus_tag="VFJ67_09085"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7290577.1"
                     /translation="MSDSKTRIASNIKDKDLINVRRQELVNAAVELFVKKGFHKTTVR
                     EIAKEFGMSMGALYDYIRTKEDILFLVCDHIHKSVSEKLQSSLVGKKDARENLRNAIR
                     DYFTIIDEIQDYSLLLYQETKSLNRTDRNYILSAEKELVAIFEGIINHGIRDKIFSID
                     RGTARIVAENIMVEGQMWAFRRWSIQKDFSLKNYIKIQTELILKSIS"
     gene            2288..3439
                     /locus_tag="VFJ67_09090"
     CDS             2288..3439
                     /locus_tag="VFJ67_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7290578.1"
                     /translation="MSILELTEEQELIQKTAKDFSAHKIRPVAAELDREGRFPGEIIA
                     KLGELGFMGIMVPEEYGGSGFDCLTYVIALEEIAVSCASTAIIMSVNNSLVCDPILAF
                     ASESQKRSYLPELSSGRRLGCFALSEPEAGSDPASMRTSALKKGKSYIINGTKSWITN
                     GAEADTAILFAATDPSKKHHGISAFIIDMDTPGVKVGKLEHKLGIKASSTAQLFFEDC
                     KVPESAILGEEGDGFKIAMSTLDGGRIGVAAQAVGIARAALEEAVRYSKERKAFGKPI
                     SNFQGLQFMIADMATKLEASRLLTWQSAVMKDRKENYSKKSAMAKLFASETAMWVTTK
                     AIQIHGGYGYTVDYPVERFFRDAKITEIYEGTSEIQRNVIARETLKEIQ"
     gene            complement(3660..4172)
                     /locus_tag="VFJ67_09095"
     CDS             complement(3660..4172)
                     /locus_tag="VFJ67_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290579.1"
                     /translation="MTRRIFYAAIACLLSASLYVIPAHSNGSPQWVTGEVVSIVENND
                     SALLSLKLADGELFSINSAPPLLEGIRIGDVVTVQVIEGRAQIINIAEYKVPATPTPE
                     KKESGVQWVPGEVVFIQEGTYDSLISVRMSDKTVFNISTPNVLLKGIQVGDHVIVKVF
                     RGWAQSISKK"
BASE COUNT         1149 a         1069 c         1180 g          912 t
ORIGIN      
        1 tggaatatcg ttctccgccg aaatcccggt agtaatcgcc cagggtgaaa gcaaacagag
       61 cgggaacgag ctgctaaagg cagttcaagc gggttctgac ttcaagggca tgaccaaaaa
      121 tgatgtcata gactcttacg gacagccttc aagcaaagat acgaccgggg agaagggaag
      181 gtacgacgag aagtggaact acagctgcga gacccataac gggctcgatt atgattgcgt
      241 gtttatctat ttcatggccg accgcgtggt tgacgtggaa actttctaac aaccggagcg
      301 ataacatgag aaagtgcccg aagtgcggaa aagagctcga atccagggtc ctggggccga
      361 tagacgttga cgagtgcgag tcgtgcaagg gcgtctggct cgatgccggg gagcttggca
      421 aagcggagga agccgccgac cccaacatca actggctcga tttcgagata tggaaacacc
      481 ccgagaaatt caagtcgaga gcatcggatc tcaaatgccc gtcgtgtctt gaaaacacgg
      541 tcgccatcga atacggtcac acaggggtcg agatcaacta ctgcccgtcg tgccggggga
      601 tatggctgga aaaggacgag ctgccgaaga tcatcgaatc actcgaagac gagatatcga
      661 aaaagaccgt atccgactat gtccacgaaa gcatacgcga ggctaaagaa atcgtgaccg
      721 gccacgaatc cttcatgtcc gagtggaagg acctgtcctc gatactgaga ctcatgcagt
      781 acaggctgtt cgtcgagaaa cccaagctga ttgcgaccct gaccgatatc aatacgctga
      841 acccgtttaa ataaccatgg tccatggaca gaggacgtgc cgtccctgac cgtaggcaat
      901 caaaacggca gattaaagcg cgcggtcgag aatccctgaa attttgtccg tatccacctc
      961 tccgctcttg tcgtataaga ccatgccgtc accgccaatt atagttacat gaggaatgta
     1021 attcctgtag aagcggctcc tcaacgcctc ctgctcttta gacaggccgg tgtcgaggtc
     1081 gacgattatg aaatcgactt tgcccttgta tttgccgtag agctcgacag tacgtctagc
     1141 ctgccgcttg gagttgtagc attcgcggtg gtaaaagatg atataggcgg gcgtcccctt
     1201 gctaagagag ccgtccgaga atcgctcacc cgtaatgaga gggccgtcgt tcgagtcgct
     1261 cgtatagtcg agggatgggt tgagcttcaa atccgtcgcc gcacgggaaa tccccgccgg
     1321 gcctgcaagc agtaataaca tgaaccccgt caatacagcc ggtcctaaat tttttccaat
     1381 cattatagtt aaccccctgt ggcggagaag tccttgtata atatacgggg taaatatctc
     1441 cggggattca ttttaattca caagacggaa gctctaaatt tatccgtcat aataaagtaa
     1501 aataaggcgt cgtggtatgt tgttcgggac gaatcgaatc ctgtgcataa tcacacccgg
     1561 aaaaggaacc gacacgatga gcgactcaaa gacccgtata gcatccaata ttaaagacaa
     1621 ggatctgata aacgtcagga ggcaggagct cgtcaacgcc gcggtggagc tattcgtcaa
     1681 aaaggggttc cataagacga ccgtccggga aatcgcgaaa gagttcggta tgagcatggg
     1741 ggcgctctac gattacataa ggacgaaaga ggacatactt ttcctcgtct gcgaccacat
     1801 ccacaagagc gtgagcgaga agctccagag ctcgctggtc ggcaagaagg acgcgaggga
     1861 gaacctccgg aacgcgatac gggactactt caccataatc gacgagatac aggactactc
     1921 gctcctcctt tatcaggaga cgaagtccct gaaccgcacg gacaggaact acatactctc
     1981 cgcggaaaaa gagctggtag cgatcttcga gggtattatc aaccacggca tccgggataa
     2041 aatattcagt atagacaggg gcacggcgag gatagtcgcc gaaaatatca tggttgaggg
     2101 acagatgtgg gcgttcaggc gctggtcgat acagaaggac ttctcactca agaactacat
     2161 caaaatccag actgaactca ttttgaagtc gatctcataa agcccttgac atagcggttg
     2221 aatgttttta gtattattag cgagcgagcg ctcggtaggt ggccggcgca aggggggtaa
     2281 acaatatatg tctatcttgg agctgacaga ggaacaggag ctgatacaaa agacggcgaa
     2341 ggacttctcg gcccataaaa tcaggcccgt cgcggccgag ctcgacagag agggaaggtt
     2401 ccccggggaa ataatcgcca agctcggaga gctcgggttc atgggaatca tggtgcccga
     2461 ggagtacggt gggagcgggt tcgactgcct tacatacgta attgcgctcg aggaaatagc
     2521 ggtatcctgc gcatcgaccg ctatcatcat gtcggtcaac aactcgctcg tctgcgaccc
     2581 tatactcgcg ttcgcttcgg agagccagaa gcgctcgtat ctgccggagc tttcctccgg
     2641 gaggaggctc ggctgtttcg cgctgagcga gcccgaagca ggctccgacc cggcaagcat
     2701 gaggacttcc gcgttgaaga aaggtaagtc ctatatcata aacggtacga agtcatggat
     2761 aacgaacggg gccgaggccg acacggcaat attattcgcc gcgaccgacc cttcaaagaa
     2821 acaccacggc atctcggcct tcataatcga tatggatacg ccgggggtca aggtggggaa
     2881 gctcgagcat aagctcggga tcaaggcgtc gagcacggcg cagctgttct tcgaggactg
     2941 caaggtgccg gaatcggcga tcctgggaga ggaaggcgac ggcttcaaga tcgccatgtc
     3001 cacgctcgac gggggacgga taggggtcgc ggcgcaggcg gtagggattg cgagggcggc
     3061 tctcgaggag gcggtcaggt attccaagga gaggaaggcg ttcggtaaac ccatatcgaa
     3121 cttccagggc cttcagttca tgatcgcgga catggctacg aaattagagg cttcgcggct
     3181 acttacatgg cagtcggcgg tcatgaagga cagaaaagag aactattcga aaaaatcggc
     3241 tatggcgaag ctctttgcct ccgagaccgc gatgtgggtc accacgaagg ccatacagat
     3301 acacggagga tacggataca ctgtagatta tccggtcgaa aggtttttca gggacgcgaa
     3361 aataaccgag atatacgagg ggacatccga gatacagaga aatgtgattg cgagagaaac
     3421 gctgaaagaa attcagtaaa aaagaagccc ttagaactaa aagggcttct tttccctcaa
     3481 ggaggatggt ataggatgtg cgcgccttat tcagccttta atttaatcac atcagtttag
     3541 acagtaaccg gcgcgggaaa gattcaggga atttcaactc cctcgatatt cgcgagctgt
     3601 aagtctccag acccccgaaa tatgcccggt tcttgcagaa gacggcgaag gaagtcactt
     3661 cattttttgg atatggactg cgcccagccc cggaagacct ttacgatcac atggtcgccg
     3721 acctgtattc cctttaacag aacgttcgga gtcgagatat tgaagactgt cttgtccgac
     3781 atccttacgc tgataaggct gtcgtaagtg ccctcctgaa tgaacactac ttctccgggg
     3841 acccactgca cgccgctttc ctttttctcg ggcgtgggcg tcgccgggac cttatactcg
     3901 gcaatgttga taatttgcgc gcgtccctct attacctgaa cggtcaccac gtctccgatg
     3961 cgaattccct ccagaagggg cggcgccgaa ttgatactga agagctcacc gtccgcgagt
     4021 ttaaggctga ggagcgccga atcattgttt tccacgatag acacgacttc tcctgtcacc
     4081 cattggggag agccgttcga atgcgccggt attacgtata atgacgcgga aagcaaacat
     4141 gcaatcgccg cataaaatat tctcctggtc atcgcgaaac ctccgtaagc agccctttga
     4201 accatccgtt atttatcgtc ctacccactt ataagtgtaa tttttatgcc ccgtttatac
     4261 aactttattt atgcgggctt aatatacgca tccgttcttg ctaacgaacg
//
LOCUS       DASSJH010000414        10288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_857156, whole genome shotgun sequence.
ACCESSION   DASSJH010000414 DASSJH010000000
VERSION     DASSJH010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857156"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..428)
                     /locus_tag="VFJ67_09100"
     CDS             complement(15..428)
                     /locus_tag="VFJ67_09100"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET7290580.1"
                     /translation="MVRILKSVYGGIIKHAEAGFPHEVCGVLVGSDGKITHYRECRNL
                     NTERAHDRYDLDPVSFKEADEWAREKGLEIVGIYHSHPDHPSIASETDRQWAWPEWVY
                     MIFSINSGRYNDARAWVLEDFDSNFMEVAVELEEG"
     gene            complement(422..1369)
                     /locus_tag="VFJ67_09105"
     CDS             complement(422..1369)
                     /locus_tag="VFJ67_09105"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET7290581.1"
                     /translation="MDYSLARKKSLETRLKKADSVIDLVGDTPLIRLTHVTEGLSPGV
                     EVYAKAEWFNPGGSVKDRPALWMILEGIRTGKLTPDKILMDSSSGNTAIAYAMIGAAL
                     GYRVELVIPENINIERKKTLQAFGSKIIYSDPLEGSDGAIRLARKLKAENPDKYFMPD
                     QYNNPENPKAHYESTAVEIWEQTEGRVTHFVAGLGTSGTFMGTSKRLKEFNPEITALA
                     VEPAEALHGLEGMKHMPTSIVPGIYDPGYPDELIRVTTEDAYDFMKELLKQEGIFVGH
                     SGGAVAFATVEYAKNLKEGVLVTIFPDGGYRYLSGGIWW"
     gene            complement(1369..1605)
                     /locus_tag="VFJ67_09110"
     CDS             complement(1369..1605)
                     /locus_tag="VFJ67_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET7290582.1"
                     /translation="METRKIDTRIDITQEICPMTYVKTKLKLETMSSGQVLEVILREG
                     EPLNNVPKSIMAEGHKILDVRQEGEYYKLLIERR"
     gene            complement(1777..1923)
                     /locus_tag="VFJ67_09115"
     CDS             complement(1777..1923)
                     /locus_tag="VFJ67_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290583.1"
                     /translation="MTKILLIVLIVLIAFWLGKMSADGKRKNLSKRNSEPEGPVIDIK
                     AEDK"
     gene            complement(1940..2665)
                     /gene="purQ"
                     /locus_tag="VFJ67_09120"
     CDS             complement(1940..2665)
                     /gene="purQ"
                     /locus_tag="VFJ67_09120"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HET7290584.1"
                     /translation="MYNFGVIVFPGSNCDYDSYHVIKHVMGQNCEFVWHEDVNLDRFD
                     CIIIPGGFSYGDYLRTGAIASLSPIMDSVEKFASRGGPVLGTCNGFQILVEAGLLPGV
                     LIRNSSLEFVCRWVDIRVENDKTPFTGSLKEGDVLRIPVAHGEGSYYCTEDVLKSLED
                     NSQIVFRYCDAEGNITPESNPNGSIGNIAGICSKAGNVLGMMPHPERCSESVLGGADG
                     RLIFESVISWLGLGQKKAKSKAR"
     gene            complement(2667..2918)
                     /gene="purS"
                     /locus_tag="VFJ67_09125"
     CDS             complement(2667..2918)
                     /gene="purS"
                     /locus_tag="VFJ67_09125"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET7290585.1"
                     /translation="MKEFRVKVEIRLKPVVLDPQGKSVLSALSNLGFGEVRDARVGKL
                     IELRISDGSAESVRERVDEMCRKLLSNPVIEDFVVNVEE"
     gene            complement(2947..3783)
                     /locus_tag="VFJ67_09130"
     CDS             complement(2947..3783)
                     /locus_tag="VFJ67_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET7290586.1"
                     /translation="MKKATLGRGLDALIPKESKPEGLTLLSINEIRPNALQPRKDFDD
                     ETITELTASIKEKGILQPIVVRQAAGGYEIIAGERRWRAAQRAGVVKVPVVVKDASDM
                     EALELALIENLQREDLNPIEEASGYQHLIEVYGLTHEEVSLRIGKDRSTVTNQLRLLR
                     LSDEAKRALIEEEITAGHARALLGLESLSDANVVLHAIRKKKLSVRMTETLIRNMTRE
                     RKSAVKPVSSDPYLERLADDIKGFLSTQVRIIYNKGKGRIEIDYYSDDELERLVSALL
                     GK"
     gene            complement(3761..4567)
                     /locus_tag="VFJ67_09135"
     CDS             complement(3761..4567)
                     /locus_tag="VFJ67_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7290587.1"
                     /translation="MGKVICVANQKGGVGKTTTVINLAASLAAAQKKTLFIDFDPQAN
                     ATSGVGINKDELEKTIYDAIIGDVNISDITVDIEPEFLKGNLRVVPASPDLTGAEVEL
                     IHLENREWKLKEAIGDVVREYDYIFIDCPPSLSLLTVNALTAADSVLVPVQCEYYALE
                     GLGQLKRTISLIRQRINPELKVEGYLLTMFDSRNKICHVVANELKTYFADEVFETVIY
                     RNVRLAESPSHGKPILLYDIKSIGAQTYISLAQEIIARNGGERIEESYAG"
     gene            4950..5780
                     /locus_tag="VFJ67_09140"
     CDS             4950..5780
                     /locus_tag="VFJ67_09140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7290588.1"
                     /translation="MNKDHLTIMLITPGSKGPKALNLKISHLKIAAFSFIFFTVVSLI
                     SFGSTYTFYKASESKTRSVRHLANTIDTLSQDLLLNKSTEAGLRNKMMNIENKLLEMQ
                     EMLDKKGIKKDLAVGGEFIPADRLSISYVDYMQKDIDDLFDTMKNLPVGSPLDGRINS
                     GFGYRKDPFRSSIGFHSGVDIDAKFGDPVVATADGVVKKTGWQGSYGKTVVLQHEEGF
                     ETIYGHLSKISVEEGQKVKVGEVIGKAGNTGRSTGTHLHYEVVKDGKRVNPSNFLSLK
                     "
     gene            5783..6250
                     /locus_tag="VFJ67_09145"
     CDS             5783..6250
                     /locus_tag="VFJ67_09145"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET7290589.1"
                     /translation="MGMFDSKKTSRPVKTETSHFTTSIGEECVFEGSISTSSSTRIDG
                     KLIGKISGENALIVGERGVILGEVKASEIIVYGRIEGIIDSERLEIKNTGIVTGDIFI
                     DRLVVEEGGIYNGRCGMSERVEQFISEPGAMEPESSVSDAAELGQTQLRAKDL"
     gene            complement(6267..6875)
                     /locus_tag="VFJ67_09150"
     CDS             complement(6267..6875)
                     /locus_tag="VFJ67_09150"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7290590.1"
                     /translation="MKKKRVLIASITVLSIVLIIWAVILYQVFAIKSKASYYGSSYDM
                     RAPDFTLTNQDGEKVTLGEFKGKVVFMFFGYTHCPDICPVTLANLSSAVNELGNDKDK
                     VRVIFVTIDPERDSQAELKKYVTYFNKDFIGLTGTPDEIKNVADSYHAFYIKEEAGSD
                     TGYLMGHTSSVYLINPEGRIVLRYPQNKMDPKEIAKDVKRIL"
     gene            7441..7512
                     /locus_tag="VFJ67_09155"
     tRNA            7441..7512
                     /locus_tag="VFJ67_09155"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7473..7475,aa:Met,seq:cat)
     gene            7622..8644
                     /locus_tag="VFJ67_09160"
     CDS             7622..8644
                     /locus_tag="VFJ67_09160"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET7290591.1"
                     /translation="MQSDTVTGDGLNTFIRALPKAELHLHVEGTLEPELLFELAERNS
                     VKLPYPSIESARKAYDFSCLQDFLDAYYQGMKVLIREEDFYDLTTAYLKKASSQNVRH
                     AEIFFDPQAHTGRGVAFETVLDGITRALRDGKRELGISSGLILCFLRDLSAASAMETL
                     RQAIPYKDRIIAVGLDSVEVGYPPGKFKGVFDVARQEGLLTVAHAGEEGPPEYIWEAL
                     DLLKAMRIDHGVQCQKDEKLMRHLIETQIPLTVCPISNVRLRIFDKLSDHNFKYLLER
                     GLCVSINSDDPAYFGGYIEENYRAVKDALGLSRSELARAAINSFTASFLSVAEKQRYI
                     DEIESL"
     gene            8809..>10288
                     /locus_tag="VFJ67_09165"
     CDS             8809..>10288
                     /locus_tag="VFJ67_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7290592.1"
                     /translation="MSVTESLNNDISGSGPSGTDLKKSRQELLDLIQQLMSIRQEMLR
                     FGERLKGSIDDLDPSYRESAKNLLHYLAFRRHDLRVLQSELAELGLSSLGRSESHVMA
                     TIEAVLNTLSRLADFPLQLTDGADVLDFKSARRLLENHTKALLGPGPKGRSVHIMVTM
                     PSEAADDYGLVLGLVNNGMNCMRINCAHDDREKWTRMIENLDRARKETGKPCKIIMDI
                     PGPKLRTGPITPGPSVIKYRPQRDAYGKVIKPANVLLAAEGTIISRLTMYDAVLPVPG
                     EWLDELSLGDEIRFVDGRGARRTMKIVEETDEGFRAETEKTAYLTPDTLLRCVKRDKS
                     GKSGVRVCGIAPRDNHITLCQGDLLTLTPDLSPGRPAEIDENGTITSPATVGCTIPEI
                     LEDVKVGEHVWFDDGKIGGIVEERIAGGLQIRIMHTSLASTKLSSDKGINFPESDLDL
                     PALTPDDIPVLEFIAEHADIAAMSFANTPEDVKSLLGHLRQMG"
BASE COUNT         2448 a         2809 c         2527 g         2504 t
ORIGIN      
        1 ccgcattgtt cctctcagcc ctcttctaat tctacagcga cttccatgaa gttagaatcg
       61 aaatcctcga gcacccaggc ccgggcatcg ttatatctgc cgctgtttat agagaatatc
      121 atatacaccc attcaggcca cgcccactgt ctgtccgttt cggaagcgat agaaggatgg
      181 tccgggtgcg aatggtagat gcccactatt tcgagaccct tctccctcgc ccattcgtca
      241 gcttccttga aagatacggg gtcgaggtca taccggtcgt gagccctttc ggtattcaag
      301 ttcctgcact ccctgtaatg cgtgatttta ccgtcagacc caactagcac gccgcagacc
      361 tcgtgcggga agcccgcctc ggcatgcttt ataatcccgc cgtaaaccga cttgaggatc
      421 cttaccacca gatccccccg ctcaggtacc tgtagcctcc gtcggggaaa atagtgacga
      481 gcacgccctc cttgagattc ttcgcgtatt cgaccgtggc gaatgcgacg gcgcccccgg
      541 agtggccgac aaaaatccct tcctgcttta gaagctcctt catgaaatca taagcgtctt
      601 ccgtggtaac tcttatcagc tcatcggggt atccgggatc gtatataccc gggactatgg
      661 acgtcggcat atgcttcatg ccttcgagcc cgtgaagtgc ctccgccggc tcgaccgcta
      721 acgctgttat ttcgggatta aactccttta gcctcttaga tgtgcccata aaggtcccgc
      781 tcgtccccag gcccgcgacg aaatgagtga ccctcccctc cgtctgctcc catatctcaa
      841 ccgcagtgga ttcgtaatgg gctttcgggt tctcggggtt attgtactga tcgggcatga
      901 aatacttgtc cgggttttcg gctttaagct ttctcgcgag tcttatcgcg ccgtccgacc
      961 cctccagagg gtccgaatat attatcttcg agccgaacgc ctggagagtc ttttttctct
     1021 ctatattgat attctcgggt ataacgagct cgaccctgta tccgagcgcg gccccgatca
     1081 tggcgtatgc tatggccgtg ttaccggacg aggaatccat aagtatctta tccggcgtca
     1141 gcttccccgt gcggattcct tcgaggatca tccagagcgc gggcctgtcc tttacggaac
     1201 cgcccgggtt gaaccattcc gccttggcgt aaacctcgac tccgggactc agtccctccg
     1261 tgacgtgagt cagcctgata agcggcgtat cccccaccag atcgattacg gaatcagctt
     1321 tcttcagtct cgtttccagt gatttttttc gtgccaggga ataatccatc aacgacgctc
     1381 aatgaggagt ttgtagtact cgccttcctg ccgtacgtcg aggatcttat gtccttcggc
     1441 cattatgctc ttggggacgt tgtttaacgg ctccccttcc ctgagtatca cctcgagcac
     1501 ctgccccgat gacatggttt caagcttgag cttggttttc acatacgtca tcgggcaaat
     1561 ctcctgggta atatctatcc gggtatcgat cttgcgagtc tccattttca acaaacctcc
     1621 ctcactatcc ccctataaaa tttaaataat tgtatcaaaa agagtaccat acgggtatgt
     1681 ccttcacaaa ccggcccagc cttccggcag ttcataaata ttgagtccta atatgtcttc
     1741 aggggtatta tactcccggg aagaggcagc aagaaatcac ttgtcctccg cttttatgtc
     1801 gatgacgggg ccctcgggtt cggaattcct cttcgacagg ttcttcctct tcccgtcggc
     1861 agacattttt ccgagccaga atgctatcag cacgatcaat acgattaaga gtatcttcgt
     1921 cataattcgg atcttccgct taccgcgcct tgcttttcgc ctttttttga cccaggccga
     1981 gccaggagat cacggattcg aatatcaatc tcccgtccgc cccgccgagg acgctctcgg
     2041 agcacctctc cgggtggggc atcatgccca gcacgttccc tgccttgctg catattcccg
     2101 ctatgttccc gatggaaccg ttcggattgg actcgggcgt tatgttccct tccgcgtcac
     2161 aatacctgaa tacgatctgc gaattgtctt cgagcgattt cagcacatcc tccgtacagt
     2221 agtagctccc ttccccgtga gcgaccggga tcctgagcac gtcgccttcc ttcaggctcc
     2281 ccgtaaatgg ggtcttgtcg ttctccaccc tgatatcgac ccacctgcac acgaactcta
     2341 gagaagagtt ccttatgaga acgcccggca gcaagcctgc ctcgaccagg atctgaaaac
     2401 cgttgcacgt cccgagcacg ggcccgcccc gtgatgcgaa cttctcgaca gaatccatga
     2461 tcggagaaag gctcgcgatc gctcccgtcc tcaggtagtc gccgtaagag aatccgccgg
     2521 ggattatgat gcagtcaaac ctgtcgaggt taacgtcctc atgccagacg aactcgcagt
     2581 tctgacccat cacatgcttt atcacatggt aactgtcgta gtcgcaattg gaaccgggga
     2641 aaacgataac tccgaaatta tacattttac tcctctacat tgactacgaa atcctcgatc
     2701 acggggttcg acaagagctt tctgcacatt tcgtcgacac gctccctcac gctttcggcg
     2761 ctcccgtcgc ttatcctgag ctcgatgagc ttccccactc tggcgtcgcg cacctctccg
     2821 aacccgaggt tggagagcgc ggaaagcacg gactttccct gcgggtcgag aacgaccggc
     2881 ttcagcctta tttcaacttt gactctgaat tccttcattt gaattccgtg aacacgtttt
     2941 tattgtttat ttcccaagta gtgcggatac gagcctctcg agctcgtcat cggaataata
     3001 atctatctcg attctgcctt tacctttatt atatatgatc ctgacctgag tgctgaggaa
     3061 ccctttaata tcgtccgcaa gcctctcgag atacgggtcc gagctcaccg gtttcaccgc
     3121 cgatttcctc tccctcgtca tattccgtat cagggtttcc gtcatcctga ccgagagctt
     3181 tttcttgcgt atggcgtgaa gaacgacgtt cgcgtccgaa agagactcga ggccgaggag
     3241 cgcgcgcgcg tgtcccgccg ttatctcctc ctcgatgagc gcccgcttgg cttcgtccga
     3301 gagcctgagc agccgcagct ggttcgtaac agtcgacctg tcttttccga tgcgcaagga
     3361 gacctcttca tgtgtgagac cgtatacctc tataagatgc tgatatcccg aagcttcttc
     3421 tatcgggttc agatcctctc tctggaggtt ctcgatgagg gcgagctcga gcgcctccat
     3481 gtcggaggcg tcttttacca cgaccgggac cttgacgacg ccagcccgct gcgccgccct
     3541 ccaccttctc tcaccggcta ttatctcata tccgccggcc gcctggcgca cgactatggg
     3601 ctggagtatg cccttctcct tgatggaagc cgtgagctcg gtaatggtct cgtcgtcgaa
     3661 gtccttcctc ggctgaagcg cattgggcct gatctcgttg atgctcagga gcgtaagccc
     3721 ttcgggtttg ctctctttcg gtatcagggc atcgagtcct ctacccagcg tagctttctt
     3781 caattctctc ccctccgttt cttgcgatta tttcctgcgc aagcgagata tatgtctgtg
     3841 ctcctatgga cttgatatca tagagcagga tcggctttcc gtgactcggt gattcggcga
     3901 gtctcacgtt tctgtaaatg accgtctcga acacctcgtc cgcgaaatag gtcttgagct
     3961 cgttcgccac gacgtggcat atcttgttcc tcgaatcgaa catcgtgagc agatatccct
     4021 ccaccttgag ctcgggattt atcctctgac gtatcagaga tatggttcgt ttcagctgac
     4081 cgagcccttc gagtgcgtag tactcgcact gcaccggcac cagcaccgaa tccgccgctg
     4141 tgagcgcgtt caccgtgagg agactcagtg acggcgggca gtcgataaag atataatcat
     4201 actcgcggac gacatcacct atcgcttcct tcagcttcca ttccctgttt tcgaggtgta
     4261 tgagctcgac ctcggcgccg gtcaggtcgg ggctcgcggg gaccactctc aaattcccct
     4321 tcagaaattc gggttctata tcgaccgtta tgtcgctaat gttaacgtcg ccgattatcg
     4381 catcgtaaat cgttttctcc agctcgtcct tgtttattcc gacgccgctc gtcgcgttag
     4441 cctgcggatc gaaatcgatg aagagggtct ttttctgcgc ggcggcaaga gacgcggcga
     4501 gatttatgac agtagtcgtc tttcccaccc cgcctttctg attcgcgaca catatcacct
     4561 ttcccatatc cctctcccct ggctgccttg aaatattttc cccgggtaat tagttcggat
     4621 aaatatatat aaatgaaacc ctttctttaa atttgagtga ctcgccgtcc gggataaata
     4681 ttaccatatt atccattatc gtatttggcc gcaagactgc cccgatcata acttccatct
     4741 ctgcctcaga gccgggagag ggagctgcta atgccctaat cctctatgag cacaccgttt
     4801 ttcaattagt atagaagaaa ttaattacgg ggttgaattt accggtgaat atagtatgat
     4861 tggaatcctg gaaatcgggg tccattctcg gattccgtgt ttggtaaccc gacgggtctg
     4921 gttgcctaat atttgtgaag gagggggata tgaataagga ccaccttacc attatgctca
     4981 ttactccggg cagtaaaggt cccaaagctc ttaaccttaa aatctcgcat ctaaaaatag
     5041 cagctttcag ttttatattt ttcaccgtcg tttctctcat atcattcgga tccacttaca
     5101 ctttctacaa ggcatcggaa tcaaagacaa gatcggttag gcacctggcc aatacaatcg
     5161 acacgctgag tcaggatctg cttttgaaca aatcgaccga agcgggactc aggaacaaaa
     5221 tgatgaacat cgagaacaag ctcctcgaga tgcaggagat gctcgataaa aaaggcataa
     5281 agaaagacct ggctgtggga ggtgaattca ttccggccga caggctgagc atttcttacg
     5341 tcgactacat gcagaaggac atcgacgacc tgttcgacac catgaagaat ctgcccgtcg
     5401 ggtcgccgct cgatggcagg ataaattccg ggttcggcta caggaaagac cccttccgtt
     5461 cgagcatagg atttcattcg ggcgtggata tcgatgcgaa gttcggcgac cccgtagtcg
     5521 cgacagcgga cggcgtagtg aaaaagacgg gatggcaggg aagctacggg aaaacagtcg
     5581 tccttcagca cgaagaaggt ttcgagacga tctacggaca cctctcgaaa atctctgtgg
     5641 aagaagggca gaaagtaaaa gtgggggaag tgataggaaa agcgggcaat accggcaggt
     5701 ccaccggcac gcacctgcat tacgaggtgg taaaagacgg caagagggtc aacccttcca
     5761 atttcctatc gcttaaataa tcatgggcat gttcgacagc aaaaaaacct caagacccgt
     5821 aaaaaccgag accagtcatt tcaccacttc tataggagag gagtgcgttt ttgaaggcag
     5881 catctcgacc tcctcgtcca caagaataga cggaaagctt atagggaaaa tatccggtga
     5941 aaatgcacta atcgtgggtg agcgcggagt gatactggga gaggtcaagg cctccgagat
     6001 catagtttac ggaaggatcg agggtatcat cgattcggaa agactcgaga taaaaaacac
     6061 cggcatagtg accggggaca ttttcattga caggctcgtt gtggaagagg gaggcattta
     6121 caacggtaga tgcgggatga gcgagcgtgt cgagcagttc atatcggagc cgggcgcaat
     6181 ggaaccggaa agttccgttt ccgatgccgc cgagctcggg caaacgcagt taagagccaa
     6241 agatctctaa tccggctgcc gcagattcac aatatccttt taacgtcctt agctatttcc
     6301 ttcggatcca tcttgttctg aggatatctg agtactatac ggccctcggg gtttatcaga
     6361 tatacggacg aagtatgtcc catcagatac ccggtgtccg aaccggcttc ctcctttata
     6421 taaaacgcgt gataggagtc ggccacgttc ttgatctcat cgggcgtacc cgtcagccca
     6481 ataaagtcct tgttaaaata agtgacgtac ttcttaagct ccgcctgact gtccctctcg
     6541 gggtctatgg taacgaagat aacccgcacc ttgtccttat cattccctaa ttcattaaca
     6601 gcgctgctca aattcgccag cgtgacagga catatatccg ggcaatgcgt gtatccgaaa
     6661 aacatgaaca cgactttccc cttgaactcg ccgagcgtta ccttctcccc gtcctggttt
     6721 gtgagcgtaa agtcgggtgc ccgcatgtcg taagagctgc cgtagtagct cgctttgctc
     6781 tttatcgcga aaacctgata aagaatcacc gcccaaatta tcaggactat cgaaagcacg
     6841 gtaatggagg caatcagaac tcttttcttt ttcatgatat ttggttttat tcgtaaggga
     6901 tgtaatgttt cggaacggat aatatattac ccgtttatca tacggcgcgc aggaagaact
     6961 attacgacac agaattccgg tattacgtta ggggggcgga tacggaattt tccggagatc
     7021 gaagagaatt agtcataaaa ataaaaggga atgcccggac cttgtcgcga gcattccctt
     7081 tttaaaggag gatggaggat gacgtgttaa gccttactat taagattagt aataaaaata
     7141 aagtatttta cttaagatgt caaggggata tttttcacaa ataaaaaatc attgcatatc
     7201 cttgtattta ttggctaatt ccatgcggaa tcattcgctc tcgcggatca aattttatag
     7261 cgattgtgaa atatgactta cacatgttat tacataccgg gctttccccg tcgagccaaa
     7321 accttgaaac ttaatcggcg cgtgatatat ttttccactc cggttttgag ataaacagtg
     7381 tgttgcgcgc ccgggaaagc gcccgatatc attagcgaga ccgattcaaa ttcactgcat
     7441 ggcgacgtag ctcaggggca gagcagggct ctcataaggc ccgtgtcggt ggttcgattc
     7501 cacccgtcgc caccctatat taatacaaac aacccaagat caatcgcccg caattcaaat
     7561 ctccgcgcac attagcaaga aacacttgcg tattgagaat atatatacct agtactaaat
     7621 aatgcagagt gataccgtaa cgggggatgg actaaacact tttatacgcg ctctgccgaa
     7681 ggcggagctg catctgcacg tcgaaggcac cctcgagccc gagctgctgt ttgagctggc
     7741 ggagcgtaac tcggtcaagc tcccctaccc gtctatcgaa tccgcgcgga aggcttatga
     7801 tttcagctgc cttcaggatt ttctcgacgc atattaccaa ggcatgaagg tgctcatccg
     7861 ggaagaggat ttctacgacc ttacgacggc ttatttaaaa aaggcttcct ctcagaacgt
     7921 ccgccatgcg gagatttttt tcgatccgca ggcgcatacc ggacgagggg tcgcattcga
     7981 aacggtgctc gacggcatca cacgggccct ccgcgacgga aagcgcgagc tcggcataag
     8041 ctccgggctt atactctgtt tcctccgcga cctgagcgca gcttcggcaa tggaaactct
     8101 caggcaggcg attccgtaca aagaccggat catcgccgtt ggtctcgatt ccgttgaagt
     8161 cggctacccg ccgggtaaat tcaaaggtgt cttcgacgtc gcccggcaag aaggtcttct
     8221 cacggtcgct cacgccggag aggaaggccc tcccgaatac atatgggaag ccctcgactt
     8281 gctgaaggct atgcgcatag accacggtgt tcaatgtcag aaggatgaaa aattgatgcg
     8341 gcaccttatc gagactcaaa tacccctcac cgtctgtccg atttcgaatg tcaggctgcg
     8401 tatattcgat aaactgagcg accacaactt caagtacctg ctcgagcgcg gtctatgcgt
     8461 ctcgatcaac tccgatgacc ccgcatattt cggcggttac atcgaagaga attaccgggc
     8521 cgtcaaggat gccctcgggc tttcgcgctc agagctcgcg cgggcagcca taaacagctt
     8581 caccgcctct tttttgagcg tggcggagaa acagcgctat atagacgaaa tcgagagctt
     8641 ataaaaagac gtgcggagca aggctcaaat aaggcccgca tcggcggtcc gattccaccc
     8701 gtcgccactt atataaatca atttcttgcc actaaagttc aaccgtgttc ttcacggaaa
     8761 attaacaata tccctgtatc gttttaaact aatatcaagg gtaccaatat gtcagttaca
     8821 gagagcctaa acaacgatat cagcggaagc ggcccgtcag ggacagacct caaaaaatca
     8881 agacaggagc tcctggattt gatccagcag ttgatgagca taaggcagga aatgctgcgc
     8941 ttcggagagc gtttaaaagg ctcgattgac gatctcgacc cctcttacag agagagcgca
     9001 aaaaaccttc ttcattattt ggccttcagg cgccacgacc tgagagtatt acagtcggaa
     9061 ttagcggagc tcggtctttc ttcgctcggg cgctcggaat cacacgtgat ggcaacaatt
     9121 gaagcggtat tgaacaccct atcgcggctc gccgactttc cccttcagct cacagacggt
     9181 gcggacgtac tcgatttcaa gtccgcgaga agattgctgg agaatcatac gaaggcgctt
     9241 ctgggccctg gacccaaggg acggagcgtc cacataatgg tgactatgcc cagcgaggca
     9301 gcggacgatt acggcctcgt cctcggtcta gtcaataacg gcatgaactg tatgcgcatc
     9361 aattgcgccc acgacgacag ggaaaaatgg actcgaatga tcgagaacct cgaccgcgca
     9421 aggaaggaaa caggaaaacc ctgcaagata ataatggaca tacccggtcc caagctccgc
     9481 acgggtccga taacacccgg cccttccgta ataaaataca ggccgcaaag ggacgcgtac
     9541 ggcaaagtga taaaacccgc gaacgtgctt ctcgccgctg aagggactat tatttcgcgc
     9601 ctgacaatgt acgatgcggt attgccggtg ccgggggaat ggctcgacga gctctccctg
     9661 ggcgacgaaa tcagattcgt cgacggacgc ggggccaggc gcacgatgaa aatcgtagaa
     9721 gagaccgatg aagggttcag ggccgaaacc gaaaagacgg cctaccttac acccgacacg
     9781 cttctacggt gcgtgaagcg ggacaagtcc gggaaatccg gcgtcagggt gtgcggcatc
     9841 gcgccgcggg acaaccacat aaccctctgt cagggagacc tgctgacact cacgcccgat
     9901 ttgtcgccgg gccgccctgc ggaaattgat gagaacggga ctatcacatc gcccgcaacc
     9961 gtaggctgca cgatacccga gatacttgag gacgttaagg tcggtgagca cgtgtggttc
    10021 gacgacggca aaataggcgg aattgtagag gagaggattg ccgggggact tcagatccgc
    10081 atcatgcata cgagccttgc ttccacaaaa ctctcatcag acaagggtat aaacttcccg
    10141 gagagtgatc ttgacctgcc tgcgctaacg ccggacgata tcccggtact ggagttcatc
    10201 gcagagcacg cggacattgc ggcaatgtcg ttcgccaata cacccgagga cgtaaaatca
    10261 ctactcggcc acctgagaca aatgggga
//
LOCUS       DASSJH010000415         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_98907, whole genome shotgun sequence.
ACCESSION   DASSJH010000415 DASSJH010000000
VERSION     DASSJH010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98907"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VFJ67_09170"
     CDS             complement(<1..1630)
                     /locus_tag="VFJ67_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HET7290593.1"
                     /translation="MKTAQDLREALRVIDGRGYASYKDIAGEYGFDDFILYIDRVQGD
                     PFASPSRVRVRVYQDAAGFPKDTYSGKSRETALRDFIARAFNSATERFSLGNRGSGKS
                     GLIMMSRPGQEILERSSVIVTHLYAEARFGMGLPAFGRTVAGKHAQAMFFDELPRIVR
                     FSMIFGNLDRDALYAHIETAEDVDFLRNEIEGLGCVSFIADGSILPRASGIDPGPMSA
                     GEAVPFKSPPSLRMDVELPNRGQITGMGILKGVTLIVGGGYHGKSTLLKAVSEGIYNH
                     VPGDGREFVVTNPNAVRIRAEDGRRIEKVDISPFISNLPFGKDTKSFSTEDASGSTSQ
                     AANILESLEAGAHVLLIDEDTSATNFMIRDHRMQELVSKDREPITPFIDMVRSLYTDQ
                     SVSTVLVMGGSGDYFDVADHVISMTEYEPSDVTSEALDIARRFASERKKEGGGPLLRV
                     TERIPLSGSFDPRKGKKEVKISAKSLRSISFGRHKIDLHALEQLVDICQTTAIGDAIE
                     YARRYMDGKRTLREVASLVMMDIAREGLDVLNPRI"
     gene            2032..2346
                     /locus_tag="VFJ67_09175"
     CDS             2032..2346
                     /locus_tag="VFJ67_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290594.1"
                     /translation="MKSILSRYFVILIAGVFVLGLAACKQAEEPAEVEEVEEVEVEEA
                     APATDMTDSMAEPTVQQTVQDTSGTEVEVEETVEETVESVEDTASPAPESSTDDTGGK
                     TE"
BASE COUNT          504 a          709 c          711 g          541 t
ORIGIN      
        1 agatgcgggg gttaagcacg tctagcccct ccctcgctat gtccatcatc acgagcgacg
       61 cgacttccct gagcgtcctc ttcccgtcca tgtagcgcct cgcgtactct atcgcgtccc
      121 ctatggccgt agtctggcag atatcgacca gctgctctaa tgcgtggagg tcaattttgt
      181 gcctgccgaa agaaatggag cggaggctct tggccgatat cttcacttcc ttcttcccct
      241 tcctcgggtc gaagctcccc gagaggggga tcctctccgt taccctcagg agcgggcctc
      301 ctccttcctt cttcctttcg ctcgcgaaac ggcgcgcgat gtcgagcgct tccgacgtca
      361 cgtccgaagg ctcgtactcc gtcatcgaga taacgtggtc ggcgacgtcg aaatagtccc
      421 ccgagccgcc cataacgagc acggtagaga cactctggtc cgtgtagagg ctcctcacca
      481 tgtctatgaa cggagtgatc ggctccctgt ccttggaaac gagctcctgc atgcggtggt
      541 cccttatcat gaagttggtc gcggaggtgt cttcgtcgat gagaagcacg tgagcgcccg
      601 cttctaacga ttcgagtatg ttcgccgcct gcgacgtgct cccgctcgcg tcctcggtcg
      661 aaaacgactt cgtgtctttc ccgaaaggga ggttcgatat gaagggcgat atgtccacct
      721 tctctatcct cctcccgtcc tccgccctta tccttacagc gttcggattc gtgacaacga
      781 actctctccc gtcgccgggg acgtggttgt atatgccctc ggagacggct ttgaggagcg
      841 tcgatttccc gtgataaccg ccccctacga tgagcgtaac ccccttgagt atccccatcc
      901 ccgtgatctg accccggttc ggcagctcta catccatcct gagtgaagga ggagacttga
      961 aagggaccgc ttctccggcg ctcatcggtc ccgggtctat gccgctggct ctcgggagta
     1021 tgctcccgtc ggcgatgaac gacacgcagc cgagaccctc gatctcgttc ctcaggaagt
     1081 ccacgtcctc tgccgtctct atgtgcgcgt agagcgcgtc tcggtcgagg ttcccgaata
     1141 tcatcgagaa cctcacgatc cttggaagct cgtcgaagaa catcgcctgc gcgtgcttcc
     1201 ccgcgaccgt gcggccgaac gccgggagac ccatcccgaa cctcgcctca gcgtagaggt
     1261 gcgtgacgat taccgaggag cgctcgagta tctcctggcc gggcctactc atcattatga
     1321 ggccgctctt gccgctcccc ctgttcccaa gcgaaaacct ttccgtagcc gagttaaacg
     1381 cgcgggcgat gaagtccctg agcgcggtct ccctgctctt gcccgagtac gtgtccttcg
     1441 ggaacccggc cgcgtcctga tagaccctca ccctcacgcg cgaaggggag gcgaaggggt
     1501 caccctgcac gcggtctatg tatagtatga aatcgtcgaa accgtattcc ccggcaatgt
     1561 ccttgtacga ggcgtagccc ctgccgtcga taaccctgag cgcttccctg aggtcttgag
     1621 cagtcttcat ggattcggga atgatatgac aattttccgc gcgtgtctat aaaaaagggt
     1681 agtgcccaaa tggcaaacaa atgtaggtgc ggcttccagc cgcgataact tttggttaaa
     1741 gaggcccagg gagaacgtga ttagttgcgt cagcttcgtt gttgctctga ccagttctta
     1801 cgcacgctca gagcagctcc ctctagggag agggaaatta aacaggggcg gacgggattg
     1861 gcatcagttg cgggcacagc taaagagcat cggtagaacc ggttatgagc ggagattcac
     1921 cgcatgaaat gttgtggaat taggggaata aatattgtat gttgtttgtc tggcgggggg
     1981 aattattaca tccgggggtc ggaacccaaa ttcacttaag gaggtacagg tatgaagtcg
     2041 atattgagca ggtactttgt cattctcatc gcgggcgtgt tcgttctggg gttagccgcg
     2101 tgcaagcagg ccgaggagcc ggccgaggtg gaggaggtag aagaggtaga ggtggaagag
     2161 gctgctcccg caacggacat gacggacagc atggccgagc ctaccgtgca gcagaccgtt
     2221 caggacacga gcggcactga agtcgaagtg gaagaaacgg tcgaggagac cgtcgagtcg
     2281 gtcgaagaca cagccagccc cgcgcctgaa agcagcacgg acgacacggg cgggaagaca
     2341 gagtaagacc ggtacgagca atgttaatta aagtcgtttg aagattaacc ggggcgggcg
     2401 ctcggtgcgt accgcctttt ttttgattat aataaccggg acacgtgatc aaataccttg
     2461 ggcaa
//
LOCUS       DASSJH010000416         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_532530, whole genome shotgun sequence.
ACCESSION   DASSJH010000416 DASSJH010000000
VERSION     DASSJH010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532530"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /gene="argF"
                     /locus_tag="VFJ67_09180"
     CDS             <1..720
                     /gene="argF"
                     /locus_tag="VFJ67_09180"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET7290595.1"
                     /translation="LGANSLYLNPNDLQLGRGETISDTSKILSSYLDGVILRTYEQER
                     LEEFAMSAAVPVINALTDLEHPTQIVSDLFTLRETGLDLGKMKFAYIGDGNNVVNSFI
                     GAAAMLGFRFSIAAPKGFEPDLKALSRTMSLGNADIEIMNDPARAASGADVLYTDVWV
                     SMGQEKDSKKRKEIFRFYQLNKKLLSCAKKGAFVMHCLPAHRGEEITNEVADGPNSII
                     FEQAENKLHMGKAILEMFMKG"
     gene            complement(779..1042)
                     /locus_tag="VFJ67_09185"
     CDS             complement(779..1042)
                     /locus_tag="VFJ67_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290596.1"
                     /translation="MKYFTALILGILFFASNAGAQAPQITDYKYNPGDALHPFKLTSL
                     ALRPPLAITNVFVKGTYWVFNTDPIRRVFNIEYSPTMNIDDDY"
     gene            1306..2424
                     /locus_tag="VFJ67_09190"
     CDS             1306..2424
                     /locus_tag="VFJ67_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7290597.1"
                     /translation="MRRGEKVYYVKDFIQTVPLPSPKKIYNELEKLGYRGQPLARKAV
                     SLASYRHVKRLKLLHTKNTPRTNLPPRPNSILMGPTGCGKTYLIELLFGEIFKLPFVI
                     IDMTKFTESGYVGDDVVNILVQLVYAANESIDIAECGVIVLDEFDKIAGAYSSARFAG
                     QGTTKDVSGYGVQRELLKILEGTDVQIPLDFGFSSRGPRALISTRDITFYAVGAFSGI
                     RNLFDRKGVGFMQALEENRGDEDSIAYNLTEEEADDITKFHMFGFLPELISRFNRIVP
                     FSPLHRSTLKEIIQIKLQNYQREFEEEGFKLHIEPNVVDFIIEEALRRQTGARGLDVI
                     ISKYLEEVAFETFGKSDEGEIVLTLDSGGVSHTVKRRA"
BASE COUNT          677 a          621 c          668 g          516 t
ORIGIN      
        1 ctcggagcga actcgctcta tcttaacccg aacgacctgc agctgggcag gggggaaacc
       61 atatcggaca catcgaagat actgtcgtcc tacctcgacg gagtgatact gagaacctac
      121 gagcaggaac ggctcgaaga gttcgctatg tcggcggcgg tgcccgtgat aaacgcgctc
      181 actgacctcg agcacccgac acaaatagtg tcagaccttt tcactctcag ggaaacgggc
      241 ctcgacctcg ggaaaatgaa attcgcatac atcggcgacg gaaacaacgt ggtcaactca
      301 ttcataggcg cggccgccat gctgggcttc cgtttctcga tagccgcgcc caagggcttc
      361 gaacccgacc tcaaagctct ctcccggaca atgtcattgg gaaatgcgga catcgaaata
      421 atgaacgatc ccgcgagagc ggcgtcaggc gcggacgtgc tttatacgga cgtctgggtg
      481 agcatgggtc aggaaaaaga ctctaaaaaa aggaaagaaa tcttcaggtt ctaccagctg
      541 aataaaaagc tcctttcatg cgccaaaaag ggagcgttcg tgatgcactg ccttccggct
      601 cacaggggag aggagatcac gaacgaagtc gcagacggac cgaattcgat catattcgag
      661 caggccgaga ataagctcca catgggtaaa gccatcctcg agatgttcat gaaaggatga
      721 ggctcaatcg aacggatcgc ttgcctaagt cgttttgcgc ccgcgatacc gaaaccgatc
      781 aatagtcgtc gtctatattc atcgtcgggc tgtactctat attgaacacc cttctaatcg
      841 gatcggtgtt gaatacccag tacgtgccct tgacgaatac gttggttatg gcgagaggcg
      901 gcctcaacgc aaggctcgtc agcttaaaag gatggagagc gtctccggga ttgtatttgt
      961 agtccgttat ctgcggagcc tgagcgcccg cgttagaagc gaaaaacaga atacccagta
     1021 tcaatgccgt gaaatacttc atgtccatca cccctttaaa gtaaagtgtt aacttctaat
     1081 atcaaattat aagttcggtc ggcaaaatag tcaacggggc ccatccggcg ttccggtcgt
     1141 ttaaattgca tacttaaagc actcaatcag aatatgcctc ttatccggtc cctcagtgca
     1201 cggcaaaaaa tgatattggc ttcccgttgc tggtataata tggaagatca cttgaaaatg
     1261 attggtaaaa ttaatagaat agtattttcc ggggctttat aaattatgag aagaggagaa
     1321 aaggtttact acgtaaagga tttcattcag accgtccctc tcccctcgcc caaaaagata
     1381 tacaacgagc tcgaaaaact cggatacagg ggtcagcccc tcgcgaggaa ggctgtatct
     1441 cttgcctctt acaggcacgt caagagatta aagctcctcc acacaaagaa cacccccagg
     1501 accaacctcc cacccaggcc caactcgatc ctcatgggcc cgacggggtg cggcaagacc
     1561 tatctgatag agctgctgtt cggggagata ttcaagctcc cgttcgtcat catagacatg
     1621 accaaattca ctgagtccgg ctacgtcggg gatgacgtcg tcaacatact agtgcagctg
     1681 gtatatgccg caaacgagag catagacata gccgagtgcg gggtgatcgt gctcgacgag
     1741 ttcgacaaga tagcgggcgc atacagcagc gccaggttcg cgggacaggg cactacgaaa
     1801 gacgtatcgg gatacggggt gcagagggaa ttactcaaga tactcgaagg cacggacgtc
     1861 cagataccac tcgatttcgg gttctcgagc cgggggccga gggcgctcat atcgacgcgg
     1921 gatataactt tctacgctgt gggggctttt tcgggcatca ggaacctctt cgacagaaag
     1981 ggagtcggct tcatgcaggc gctcgaggag aacagggggg acgaggattc catagcctat
     2041 aacctcaccg aggaagaggc ggacgatatt acgaagttcc acatgttcgg gttcctgccg
     2101 gagcttatat cgaggttcaa caggatagtg ccgttctccc ctctccacag gagcacgctc
     2161 aaggaaatca tccagatcaa gctgcagaat taccagaggg aattcgagga ggaggggttc
     2221 aagctgcaca tagagccgaa cgtggttgat ttcataatcg aggaggcgct caggagacag
     2281 acgggcgcta ggggcctcga cgtcatcata tcgaaatacc tggaagaggt cgctttcgag
     2341 acgttcggaa agagcgacga aggggaaata gtgcttacgc tcgattcggg cggggtctcc
     2401 cacacagtaa aaagacgcgc atgaagatat cgatagtaag cctcggctgc tcgaaaaacc
     2461 ttgtagacag cgagataatg ac
//
LOCUS       DASSJH010000417         4640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1282378, whole genome shotgun sequence.
ACCESSION   DASSJH010000417 DASSJH010000000
VERSION     DASSJH010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282378"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFJ67_09195"
     CDS             <1..316
                     /locus_tag="VFJ67_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290598.1"
                     /translation="KGNGRVRDNIDTIKDEVERLRHEFSNVSDNILDISRKAYKSGNK
                     RLGKEAARLHAELGSGLERARRAGSGGIGAVEERIVRKPFLSVLIAFLAGVLAGKLMD
                     RE"
     gene            361..846
                     /locus_tag="VFJ67_09200"
     CDS             361..846
                     /locus_tag="VFJ67_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET7290599.1"
                     /translation="MEIVHIVNSLPLELKILSYVVISMLLGAAVGLDRELADKPAGLR
                     THMLVAGSSALLVSLGKLIVPDFGLTDVALGSDPLRIIGAIITGVSFLGAGTIIRRGS
                     EDRVKGLTTAAALLFVASVGICVALSKPLLAVGVTVIALATLRGVVYLERKFGFGKRE
                     L"
     gene            complement(843..2183)
                     /locus_tag="VFJ67_09205"
     CDS             complement(843..2183)
                     /locus_tag="VFJ67_09205"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7290600.1"
                     /translation="MPSATNDAHRHRVVILGGGFGGLYAAKALSRAGLCVTLIDRRNF
                     HLFQPLLYQVAAGWLSPGEIASPLRMVLSRFPHTYVYQAEVTDIVPDEKAVVLRDGIV
                     TYDTLIVATGSSHHYFGHEEWARTAPPLKTVEDALEMRRKILLPFEAAEREPDPAVRK
                     EWMTFLIIGGGPTGVELAGTIGELARATLRKDFRNIDTSRAEIILVEGRDHVLPSYPE
                     ELSRKAEESLRRLGVTIRLDTLVTGIEGNKAILSRKGVREEIESRTMLWTAGVKASPL
                     GRVLEKRAGAPLDGTGRVIVEPDLTIPGHPDIFVIGDLANFSHQGGAPLPGVAQVAMQ
                     EGRYAADLIKSRIAGKKKGPFRYWDKGNLAVIGRNAAVADLPWLKLSGFPAWLIWALV
                     HISYLIEFDSKVLVLFQWAWNYFTRKRGVRLITGEYPSSLVNNKDVKRAGTEKP"
     gene            2407..4245
                     /locus_tag="VFJ67_09210"
     CDS             2407..4245
                     /locus_tag="VFJ67_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7290601.1"
                     /translation="MDEHFLIGFATIIVLGIGAYWVSWRLRIPAILLLLASGFVAGPV
                     TGLINPDEIFGDILLPVVTASVAVILFEGGMNLKFSELREIRKVVISLVTVGVLVTWA
                     VTAVAAYFLFGFSHQIASLLGAILVVTGPTVIMPLLRNLRPTSRMGSIMKWEGIVIDP
                     IGAMLAVLVFQAVVAGGVIEATEKVGLSVLKTVIVGGAVGVAGGRALIIIMKRYWVPD
                     YLLNSVTLMILIAAFTFSDFIQKESGFVTVTIMGIVLANQKEVSIKHIMEFKDTLRVL
                     FISSLFIILSSRLTYGDLQFLMSYKSALFLLVLFVIARPLCVFLSTRGSGLQLKEKLF
                     LSWMAPRGIVAASVASVFGLKLVDDGFAQAQHLVSITFLVITATVLVYGLTAPPLARK
                     LGVAKPDPQGVLFVGAHDWARAMAKFLHEDGFKVSLVDSSWENVSTARQEGLMAHYKN
                     IILDDVAEHVDCEGIGKLLALTSNDEVNSLAALSFVESFGRSQVYQLPRQSGRHSAHE
                     ENVVSELTGRFLFSPEATYEFITFQFGTGAVIKKTQITAEFDYEAFRSHHGGKVVPLF
                     LIKENGDLLAFANDNPPAPQPGHHLVALVAEITERREGNALVEGAP"
     gene            complement(4334..4600)
                     /locus_tag="VFJ67_09215"
     CDS             complement(4334..4600)
                     /locus_tag="VFJ67_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290602.1"
                     /translation="MSEEERIEEIREAAEAVEEKVLAKEAEQAARPKVHSHAGKGLME
                     RRASFEQSSVPNAHRGYSGKTPRVMMGIIAILFIVSFILAGITG"
BASE COUNT          872 a         1361 c         1370 g         1037 t
ORIGIN      
        1 gaaaggcaac ggacgggtca gggacaatat agatacgatc aaggacgagg ttgagagact
       61 gaggcacgaa ttctcgaacg tatctgacaa catactcgat ataagccgga aggcgtataa
      121 atccgggaac aagagactcg gcaaggaagc ggccaggctc cacgcggagc tgggctcggg
      181 gctggagcgc gcccgcaggg ccgggagcgg gggtatcggg gcagtcgagg aacggatcgt
      241 gcggaagccg ttcctgagcg tactgatcgc ttttctcgcg ggtgtgctcg cagggaagct
      301 catggaccgg gaatgaccgg cattctttta tccgcaacct aggcacggga ggcgcatatt
      361 atggaaattg tgcatatagt aaattccctc ccgctcgagc ttaagatact atcctacgtc
      421 gtcatatcga tgcttctggg cgctgcggtg ggcctcgaca gggagctcgc cgacaagccc
      481 gcgggactca ggacccacat gctggtcgcg gggtcttccg cactcctcgt ttcgctcggc
      541 aaattaatag tgcccgactt cggacttacc gacgtcgccc tcggctcgga cccgctcagg
      601 ataataggcg caataatcac cggagtgagc ttcctcggcg cgggcacgat cataagacgc
      661 ggcagcgagg acagggtgaa gggtctcacg accgcggcgg cgctcctctt tgtcgcttcg
      721 gtaggcatat gcgttgctct ctcgaagccc ctgcttgccg tgggcgtgac agtcatcgcc
      781 ctcgcaaccc tgcggggcgt cgtttacctc gagagaaagt tcggtttcgg aaaaagagaa
      841 ctttagggct tctcagtgcc ggcgcgtttg acgtccttgt tattgacgag ggatgacggg
      901 tactcgcccg taatcagcct cactcccctt ttcctcgtaa agtagttcca cgcccactgg
      961 aagagcacaa gcaccttgct gtcgaattcg atcaggtagc ttatgtggac gagtgcccat
     1021 atgagccacg cagggaatcc tgaaagcttc aaccacggga ggtcggcgac ggcagcgttc
     1081 cttccgatca cggcgaggtt ccccttgtcc caatatctga acggcccctt tttcttcccc
     1141 gcgatccggc tctttattag gtcggctgca tatctcccct cctgcatagc cacctgggct
     1201 acacccggaa gcggcgcgcc gccctggtgg gagaagttcg cgaggtcccc gatgacgaat
     1261 atgtccggat gcccgggtat cgtgaggtcg ggttcgacga tgaccctccc cgtgccgtcg
     1321 agcggggctc ctgccctttt ctcgagcacc ctgccgaggg gcgacgcctt cacgcccgcc
     1381 gtccagagca tcgtcctcga ttctatctcc tctctcacgc ccttcctgct gagtatggcc
     1441 ttattcccct ctatccccgt gaccagagta tcgagcctta tcgttacccc tagcctgcgg
     1501 agcgattcct cagccttccg cgagagctct tccgggtagc tcggcagcac gtggtccctg
     1561 ccctcgacga gtatgatctc ggccctggag gtatcgatgt tcctgaaatc ctttctgagc
     1621 gtcgcacgcg caagctcgcc tatcgttccc gcgagctcga cccccgtggg gcctcctccg
     1681 attatgagga acgtcatcca ctcctttctc acggcagggt cgggctcgcg ctcggccgcc
     1741 tcgaacggga gcagtatctt ccttctcatc tcgagcgcgt cttcgaccgt cttgagcggc
     1801 ggcgctgtcc gggcccattc ctcgtgtccg aagtagtggt ggctcgagcc cgtcgctact
     1861 atgagcgtgt cgtaggtgac tatgccgtcc ctcagcacta cggccttctc gtcgggcact
     1921 atgtccgtca cctcggcctg atagacgtac gtgtgcggga acctgctcaa caccatccgg
     1981 agaggggagg ctatctcgcc cggcgagagc cagccggccg cgacctgata gaggaggggc
     2041 tggaagagat ggaagttcct cctgtctatg agcgtcacgc acagacccgc gcgggagagc
     2101 gccttcgccg cgtagagccc gccgaaccct ccgccgagaa tcactacgcg gtgcctgtgt
     2161 gcgtcattgg tcgctgaagg catacatttt agtattcctg acggccgggg aattctgaaa
     2221 tctgtttctc gcccggcccg gaaatatcgg gattcaccgt ccgccccgga atagatatgg
     2281 aataaatatc tgaaatttca tacaatagtc ttagccgtcc tgtatatata cttacggcat
     2341 gatcttgggt atgtgcatac gctggcaagg cgggccggac gtacgggtat atcccgggga
     2401 tattccatgg atgaacattt tctaatagga ttcgctacga taatcgtgct cggcatcgga
     2461 gcctactggg tctcctggcg cctcaggata cctgcgatac tcctgctcct ggcctccggg
     2521 ttcgtagcgg gccctgtcac ggggctgata aaccctgacg agatattcgg ggacattctc
     2581 ctgcccgtgg taacggcctc ggtcgccgtg atacttttcg agggcgggat gaacctcaag
     2641 ttctccgagc tgagggagat cagaaaggtg gtcataagcc tcgtgaccgt aggcgtactg
     2701 gtcacgtggg ccgtcacggc tgtagccgcg tatttcctct tcggcttcag ccaccagata
     2761 gcgtccctcc tgggcgcgat cctcgtcgtt acggggccta cggtaatcat gcctctgctc
     2821 cgtaatttaa ggcccacgag caggatgggc tcgattatga aatgggaagg gatagtgatc
     2881 gaccctatcg gggcgatgct cgctgttctc gtgttccagg cggtcgtagc gggcggggtg
     2941 atcgaagcga ccgagaaggt cggcctgagc gtgctcaaga ccgtaatcgt cgggggcgcg
     3001 gtcggcgtcg caggggggcg cgccctgata atcataatga agaggtactg ggtccccgat
     3061 tacctcctga actcggtcac gctcatgatc ctcatcgccg cttttacgtt ctcggacttc
     3121 atacagaagg aatcgggatt cgttacggtc acgataatgg gtatcgtgct ggctaatcag
     3181 aaagaggtga gcataaagca catcatggag ttcaaggata cgctccgcgt gctctttatt
     3241 tcgagcctct tcattattct ctcctcgagg ctcacgtacg gcgacctgca attcctcatg
     3301 agctataaaa gcgctctctt cctcctcgtg ctgttcgtta tagccaggcc gctgtgcgtc
     3361 ttcctctcca cgcgcgggag cggtctgcaa ctgaaagaga agttattcct ctcctggatg
     3421 gctccgcgcg ggatcgtcgc ggcttcagtc gcttccgtct tcggacttaa gctcgtggat
     3481 gacggcttcg cccaggcgca gcaccttgta agcatcacgt tcctggtgat cacggcgacg
     3541 gtccttgtgt acggcctcac ggcgcctcct ctcgcgagga agctcggggt agcgaagcct
     3601 gatccgcagg gcgtgctctt cgtaggcgcg cacgactggg ccagggcgat ggcgaagttc
     3661 ctccatgaag acggcttcaa ggtatcgctc gtcgactcca gctgggagaa tgtgtccacg
     3721 gcgcggcagg aggggctcat ggcgcattat aaaaatatca tcctcgacga cgtggcggag
     3781 catgtagatt gcgaagggat tggaaagctc ctcgcgctca cttcgaacga cgaggtgaac
     3841 tcgctcgctg cgctcagctt cgtggaatcg ttcggcaggt cccaggtgta ccagctcccg
     3901 agacagtcgg gccggcacag cgcgcacgag gagaacgtag tgtcggagct gaccgggagg
     3961 tttttgttct cccccgaagc gacctacgaa ttcattacgt ttcagttcgg aaccggggcc
     4021 gttataaaaa agacgcagat aaccgcggaa tttgactacg aggcgttcag gagtcatcac
     4081 gggggtaaag ttgtgccgct ctttcttatc aaggagaacg gagacctcct ggctttcgcg
     4141 aacgataacc cgcccgcgcc tcagccgggc catcacctcg tggcgctcgt cgccgagatt
     4201 acggagcgaa gggaaggaaa cgccctggtt gagggagcgc cctgagtctc gtaaggtcct
     4261 gggtaatgtc ttaaagcgtg gcttgagata acgtcggatg tctttcggac ggatattttg
     4321 aggaaagcgg cgttcacccg gtgatgcccg cgagtataaa actcacgatg aagaggatcg
     4381 ctattatgcc catcatgaca cggggagtct tcccgctgta gcccctgtgc gcgttgggca
     4441 cgctcgactg ttcgaagctc gctctgcgct ccatgaggcc ttttccggcg tgggagtgca
     4501 ctttcggcct ggccgcttgc tcggcctctt tggccaagac cttttcctcg acggcctcgg
     4561 ccgcctctct tatctcttcg atgcgctctt cttcgctcat attgtccttc gtctccgccg
     4621 tatgtcagcc gttcagtccg
//
LOCUS       DASSJH010000418         7271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1610829, whole genome shotgun sequence.
ACCESSION   DASSJH010000418 DASSJH010000000
VERSION     DASSJH010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610829"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFJ67_09220"
     CDS             complement(<1..351)
                     /locus_tag="VFJ67_09220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7290603.1"
                     /translation="MSYATIFAICMLLTFVVINLTVIKTNADEKKNPAEVQKTASGLE
                     YIIIEEGSGPKPKTGQKVKVHYTGKLEDGTVFDSSVKRGEPIEFVLGVGQVIPGWDEG
                     IADMKVGGKRQLIIP"
     gene            616..1401
                     /locus_tag="VFJ67_09225"
     CDS             616..1401
                     /locus_tag="VFJ67_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7290604.1"
                     /translation="MGFEFISYEREPNNIGILKINRPNVLNALNWDTLGELREFLEDV
                     MPKEELKALIITGAGDKAFVAGADIAQMNEMKERDFQDYVDYAHRVYELIENEPCPSI
                     AAINGYALGGGCELALACDIRIASDKARLGFPEVKLGIFPGWGGTQRVTRVLGLGKTK
                     ELVFTGEMVNAEEALRIGLVERVVPHAEIMNEAKKLAGEIAKRGPIAVRLSKTAINAG
                     SEMDLQKALLLEKTLVSLCFDSQDRIEGMKAFLEKREPLFTGR"
     gene            1621..2376
                     /locus_tag="VFJ67_09230"
     CDS             1621..2376
                     /locus_tag="VFJ67_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HET7290605.1"
                     /translation="MLEQMHDLSGKVAIVTGGSRGIGKAICLALAEAGADILLNYSRS
                     DKEADKVKKDVEKFGRKCVTVRANVGNFQQAQLLGKAVMNHFGRVDILVNNAGINRDR
                     TLKRMTYEQWNEVIQTNLGSVFNCTKAVVDPMSEHGGVIVSISSIIGEMGNVGQCNYA
                     STKAGIIGFTKTMAKELSGSNIRVNAIAPGFVETDMLGTVPKDIRDQIVKEIALGRFG
                     TPEEIALAVVYLCSSAASWITGVTLRINGGQYI"
     gene            complement(2470..3537)
                     /gene="phaC"
                     /locus_tag="VFJ67_09235"
     CDS             complement(2470..3537)
                     /gene="phaC"
                     /locus_tag="VFJ67_09235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HET7290606.1"
                     /translation="MGNKYVDSAMQEAGKAREKFNKALEIALLNPPEVEVGTTPHEVV
                     FTENKLRLLHYYPLAEKTSSVPLIMIFALVNRPYILDLKPGRSVVEVLLKKGIDVYLI
                     DWGAPGDEDKKLDLNHYINRYIKHVVKQVKKHSGSDKVNLLGYCMGGTMSAIYTAFYP
                     DDVKNLILMTAGIDYKVEGTLNLWGDKPNFDVDKFVDAFGNVPAEFLQSGFLFMKPIQ
                     NLVSKYVNFYENVDNEAFVENFLAMEKWLNDNIPVPGEVFREFVKYLYQENQLVENKL
                     RINGKQVNLKDIKCPVLNLIAENDHLVPPASSLGFNELTSSKDTKTVVFPTGHIGLSV
                     SSKSLKGLWPEVADWMIERSK"
     gene            complement(3626..4069)
                     /locus_tag="VFJ67_09240"
     CDS             complement(3626..4069)
                     /locus_tag="VFJ67_09240"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HET7290607.1"
                     /translation="MSKENFDYTEHYKKFYEAWEKTMSEAMDMWKKNPLAKGEAAESK
                     EDYDPIAQYKKFYDTWEKSSSEVLEKWVNSPLFAANIGKAVEKSSEFKKYFDEAVEKT
                     LKNMRFPTKSDIDRVLSSINNLEAKVNDLLDKMDDISKPARTTKK"
     gene            complement(4066..4452)
                     /locus_tag="VFJ67_09245"
     CDS             complement(4066..4452)
                     /locus_tag="VFJ67_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290608.1"
                     /translation="MNKKNEYEQIYYFSYFKKLYESWEESTNKLMDVWMGSPLMERAV
                     EKSSEFKNYIHNFMENTLEKRYLPEKRDMEKLIDTLDSLGEKIAELEDRLTDLQSSAK
                     KSGTKPKPKPKTKTGNAKKKVKEKKS"
     gene            complement(4555..4977)
                     /locus_tag="VFJ67_09250"
     CDS             complement(4555..4977)
                     /locus_tag="VFJ67_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290609.1"
                     /translation="MPQVKKSAPKITPKAKVEGSKEVFQDLHGLVKENYLIGLDSAHS
                     LLEENKRFMDAQIEQLRKVQNDYTEQVKSVFDKLPKEYSGLGIGAKLDRLIEFQNNFY
                     SVFKKISDNYSKEMLDLNQKSAERVFSALDKYVSSFTS"
     gene            5265..6410
                     /locus_tag="VFJ67_09255"
     CDS             5265..6410
                     /locus_tag="VFJ67_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7290610.1"
                     /translation="MKRKLVLLLLIIISALTLHARSQVIAPDGKDGQAPQKVVTFGCL
                     LPLTGKYSLLGQKALKGILAAADSGTAGTQYRVIVKDFGDSDRQLASSLDELLKTPDL
                     SFIIGPVPSKYIRAVSGGINNREIPTVVFPVSEDEDAGGPYIIKYYYPLEEQVRVLSA
                     YAVRGLGVRRFAVLYPGTPFGGRLKELFAADAAENGGKIVYEGSYDPESRDILKEIGW
                     IRSAAPEAVFIPDGAASSAELILRLKREGKLRDVLFLGPSSWNSPLFLSLIGNEIDGF
                     VYRAIFTDVFYYGDEDWDGFSKLTRGSPVEKPDIFGYQTYRAVKLMLSLDIKPGGGET
                     TADILQPIKNDPSYNIELDESGSYRVSPRYRILSVSDGELINIINVR"
     gene            complement(6453..>7271)
                     /gene="ccsA"
                     /locus_tag="VFJ67_09260"
     CDS             complement(6453..>7271)
                     /gene="ccsA"
                     /locus_tag="VFJ67_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET7290611.1"
                     /translation="NIDMDLYWVTALLYFISSVFYMSFIWTDSRTGSKVGFYTVVLGT
                     VVQAASIVALYLKGQILTGGLDTTLYIFALAITIVFIGSQLIFNAPLLGAFVSPLAFI
                     MTLPHVILPKGIIEHEPSLSNPWIVIHIVLILLGEALFTVAFISGLMYLFQEQRIKSK
                     HVGSLLQKFPSLTTLDKINHVCLIVGFPFITIGLALGLLLAKEIWGEAWVWGQKETWS
                     LVTWLLYAVLIHGRLSSGWKGRKAALGAVLGFGIILFTLFVIGYLAPGRHDFLG"
BASE COUNT         1710 a         1797 c         1886 g         1878 t
ORIGIN      
        1 ggggataatc agttggcgct tccctccgac cttcatgtcg gcgatgccct cgtcccagcc
       61 cggtatgacc tgtccgacgc cgaggacgaa ctctatcggc tcccctcgct tgaccgaact
      121 gtcgaatacc gtcccgtcct cgagctttcc tgtatagtgg actttgacct tctgtcccgt
      181 cttgggtttc ggaccgctcc cttcctctat aatgatatat tcgaggccgg aggcggtctt
      241 ttgaacctcc gccgggttct tcttctcatc cgcattcgtc tttataacgg taaggttgat
      301 aacgacgaac gtaagaagca tgcaaatcgc gaatattgtc gcatagctca tcttggtgct
      361 ctccttttga cttttcttta tttctacgaa ttccggaagt gagaaacaca ttacacaatt
      421 atcttccgga acgccatgta ccggcaaact ttccaatagg gccgggggcc cgggaggtta
      481 cggagtttgt ttatgaaaat cctgttttgg gaaatgctgc tcaaggggac aaaccccggg
      541 attgacaagt actttttacc ctgaattaat atttccggta ggggtggtca attaaaatcg
      601 cggaggagga atcccatggg atttgaattt ataagctacg aaagagagcc taacaacata
      661 ggtattctca aaataaaccg tcctaacgtg ctgaacgcgc ttaactggga cacgctcggg
      721 gagctgagag agtttctcga ggacgtaatg ccaaaggaag aactgaaagc cctcattatc
      781 acgggcgccg gggataaggc tttcgtcgcc ggggccgata tagcgcaaat gaatgagatg
      841 aaggaaaggg acttccagga ctacgtcgat tacgcccaca gggtttacga gcttatagag
      901 aatgagccct gcccctccat agccgcaata aacggatacg ccctcggcgg tggatgcgag
      961 cttgccctcg cgtgcgacat caggatagcc tccgataagg ctagactcgg gttccccgag
     1021 gtaaagctcg gcatatttcc cggttggggc ggcacacagc gcgttacgag agtgctgggt
     1081 ctcggaaaga ccaaagagct cgtgttcacc ggagagatgg taaacgcgga agaagcgctc
     1141 aggatagggc tcgtcgagag ggtggtgcca cacgccgaga taatgaacga ggcgaagaag
     1201 ctcgccggag aaatagcgaa aagaggaccc attgccgtga gactgtccaa gactgcgatc
     1261 aacgcgggct cggaaatgga tctgcagaag gcgctgctgc tcgaaaaaac gcttgtttca
     1321 ctatgcttcg attcccagga caggatagaa ggaatgaaag cgtttctcga gaagagagaa
     1381 cctttgttta ccggacggtg attgtccgcg cagctctccg gcattttcta ccggcattaa
     1441 aaaaatcttg atataagctc tccctccggg gatatcatgg caccttttca tcctgaagag
     1501 atgccgaacc tccctataaa ccctttctgc tcacctttaa ggttgacaaa aacctcaata
     1561 acaataaaat ctataatcct taattcaaac gactgagtcg ggagctccag gaggtgtctc
     1621 atgcttgaac aaatgcacga tttgtcggga aaagtagcaa tagtaacagg cggttcgaga
     1681 ggtataggaa aggctatatg ccttgcgctc gctgaggcgg gagcggatat cctcctcaat
     1741 tattcgcgga gcgataagga agccgacaag gtgaaaaagg atgtagagaa attcggaagg
     1801 aaatgcgtta ctgtgagggc gaatgtcggt aattttcagc aggcccagtt actgggcaaa
     1861 gctgtcatga accacttcgg cagggtcgac atactcgtca acaacgcagg tataaacagg
     1921 gacaggactc ttaagagaat gacatacgag cagtggaacg aagtaatcca gactaacctc
     1981 ggcagtgtgt ttaactgcac caaggcggtg gtcgatccca tgtccgagca tgggggcgta
     2041 attgtctcca tatcttccat cataggagaa atgggcaacg tcggccagtg caactacgct
     2101 tcgacgaagg cgggcataat agggtttacg aagacgatgg cgaaggagct ctcgggcagc
     2161 aacatacgcg tgaacgccat agccccgggg ttcgtggaaa ccgacatgct gggtacggtc
     2221 cctaaggata taagggatca gatcgtaaag gagattgcgc tcggcaggtt cgggactccc
     2281 gaagagatcg cgctcgctgt cgtttacctc tgctcatcag ctgcgagctg gatcaccggt
     2341 gtaacgctca ggattaacgg cggccagtac atctaaattg tataaaaaaa ggttccgtat
     2401 tcgatgtgat atatctgatg agatactcac gcgaagacgg aacctgattt tccatcctta
     2461 cgatatttct tatttggatc tttctatcat ccagtcggct acctcgggcc ataacccttt
     2521 gagagatttg ctgcttacgg aaaggccgat atgtccggtc ggaaaaacta cggtttttgt
     2581 gtctttgctc gacgtcaatt cgttgaatcc caggctcgaa gcgggcggaa caagatggtc
     2641 gttctcggcg ataaggttta acaccggaca ttttatatct ttaaggttca cctgtttgcc
     2701 gtttatcctc agcttattct cgacgagctg gttttcctga tacaggtatt taacgaactc
     2761 cctgaagacc tctcccggta cggggatatt atcgttgagc catttctcca tggcgaggaa
     2821 gttttcgacg aacgcctcgt tgtccacgtt ctcgtagaaa ttcacgtact tggagacaag
     2881 gttctgtatg ggtttcataa ataagaatcc cgactgcaga aattcggccg gaacattccc
     2941 gaatgcgtcc acgaatttgt ccacgtcgaa attgggcttg tcgccccaga ggtttagagt
     3001 gccttccacc ttgtaatcta tcccggctgt catcaaaatc aggtttttca cgtcgtccgg
     3061 ataaaatgcc gtgtatattg ccgacatcgt cccgcccatg cagtacccga gcaggttcac
     3121 cttgtcggag ccggaatgct tcttgacctg tttcacgaca tgttttatat acctgttgat
     3181 gtaatgattg aggtcgagtt tcttgtcctc gtcccccgga gcgccccagt ctatgaggta
     3241 aacgtctatt cctttcttga ggagcacttc aacgacgctc cttccgggct tgaggtcgag
     3301 tatgtaagga cggtttacga gcgcgaatat catgatgaga ggaacagagc tcgtcttctc
     3361 ggcgagcggg tagtagtgca gcaggcgaag tttattctca gtgaacacga cctcgtgtgg
     3421 agtagtaccc acctcgactt cgggcggatt cagaagcgct atctcaagag ccttgttgaa
     3481 cttctctctt gcctttcccg cttcctgcat agcggagtca acatatttat ttcccataat
     3541 tatcgagatc ctccttttct agaacgtcgc accaacggtt attctcgatg ttgatacggt
     3601 aacggacggg gtgcggacgc aggtgttatt tcttcgtagt gcgcgcgggc ttgctgatat
     3661 catccatctt atcgagcagg tcgttcacct ttgcttcgag gttgttgatg gaagaaagca
     3721 ccctgtctat gtcgcttttt gtcgggaaac gcatgttctt aagcgttttt tcgaccgctt
     3781 cgtcgaagta ttttttgaac tcggatgatt tttcaaccgc cttcccgata ttggcggcga
     3841 acagagggct gttgacccat ttctcgagta cttcggacga ggacttttcc catgtgtcat
     3901 aaaacttttt atactgggct atcggatcgt aatcttcttt gctctcggcc gcctcgcctt
     3961 tagcgagcgg gttcttcttc cacatatcca ttgcttccga catggtcttt tcccacgcct
     4021 catagaactt cttgtagtgt tctgtatagt cgaaattttc tttgctcatg attttttctc
     4081 cttaactttt tttttggcgt ttccggtttt ggtcttgggt ttgggtttag gtttggtccc
     4141 ggactttttg gcgcttgact gcaggtccgt gagcctgtct tcgagctcgg ctatcttctc
     4201 gccgagagag tcgagggtgt cgatgagttt ctccatatca cgcttctcgg gcaaatacct
     4261 cttttcgagc gtattctcca tgaagttatg aatgtagttc ttgaactcgg aggatttctc
     4321 taccgccctc tccatcagcg ggctgcccat ccagacgtcc atcagtttgt tagtggattc
     4381 ttcccaagac tcgtacagtt tcttgaaata gctgaagtag taaatctgct cgtattcgtt
     4441 cttcttgttc aaactgtgtt cccctgacgg gcgaaccccg aagccctaat aaccttccat
     4501 atattatact acatattaaa aatcataaat cacacagcga agccgctctg cgggtcagga
     4561 ggtgaaggag ctaacgtact tatccaaagc cgaaaaaacc ctctcggcgg atttttggtt
     4621 cagatcgagc atctcttttg agtaattatc cgatattttt ttaaacacgc tgtagaaatt
     4681 gttttgaaac tctatgaggc ggtcgagctt tgcgcctatt cccaggcccg agtattcttt
     4741 cggcagcttg tcgaagaccg atttgacctg ttccgtgtaa tcgttctgga cctttcttag
     4801 ctgctctatc tgcgcatcca tgaacctctt gttctcttcg agcaatgaat gggcggaatc
     4861 gagcccaatg aggtaatttt ccttcacgag accgtgcaaa tcctggaata cttctttact
     4921 tccctctacc ttggctttgg gtgtgatttt tggtgctgat ttcttgactt gcggcatttt
     4981 tttacccccg atttattctt tctattaata taattagcta cgtttttttg tcaagtcgtt
     5041 atgttagcaa ctgcacaatt atattatata atcatacaag ccggttatat ggccgagacg
     5101 tcgtatctca tagcattagc gttaacctgc cgggcgaggg gggtattgcc ccgacggcgg
     5161 cagttccgcg gcgaatgtct ttatcggcgt gcccgggtgt gccttaaccg cctgtcttta
     5221 tgtcctgaat ccggtaacat tttgtccgtt gcgggagatc cctcgtgaag agaaagctcg
     5281 tccttctatt gctcataatc atttccgctc taaccctgca tgcccggtct caggtaattg
     5341 cgcctgacgg gaaagacggt caggcgcctc aaaaagtggt gacgttcgga tgtctccttc
     5401 cccttacggg caagtatagc ctgctcgggc aaaaggctct caagggtatc ctggccgcgg
     5461 ccgattccgg gaccgcaggc acgcagtaca gggtcatagt caaggatttc ggagacagtg
     5521 acagacagct ggcctcctct ctcgatgagt tgctgaaaac accggacctg tcgttcatta
     5581 tcggccccgt ccccagtaaa tatatccggg ccgttagcgg gggcatcaat aacagggaaa
     5641 ttccgaccgt cgtgtttccg gtctcggaag acgaagacgc ggggggtcct tatataataa
     5701 agtattacta ccctctcgag gaacaggtca gggtgctgtc ggcatacgca gtccgcggtc
     5761 tgggggtgag gaggttcgct gtcctatatc ccggaacccc ctttgggggc aggttgaagg
     5821 agttattcgc tgcggacgcc gcggaaaacg gaggcaagat cgtctacgag gggtcgtacg
     5881 acccggaatc gagggatatt ttgaaggaga taggctggat caggtcggcc gcgcccgagg
     5941 ccgtgttcat acctgacggg gccgcatctt ctgcggagct gattctgagg ctcaaaaggg
     6001 aaggcaagct cagggacgtc ctcttcttag gaccgagctc atggaacagc ccgctcttcc
     6061 tgagcctcat agggaatgag atagacgggt ttgtgtacag ggcgatattc accgatgtct
     6121 tttattacgg cgacgaggac tgggacgggt tttcaaaact gacacgggga agtcctgttg
     6181 agaagcccga tattttcgga tatcagacgt atagggcggt aaagttgatg ctctctctcg
     6241 acattaagcc cggaggcgga gaaactacgg cggacatact tcagccgatc aaaaacgatc
     6301 cttcctataa tatagagttg gacgagagcg ggagctaccg ggtctccccg cgttacagga
     6361 tcctctccgt gagtgacgga gagctgataa acataattaa cgtcaggtga tcagtaaaca
     6421 ttgacggctt ccgaatctct ttagcgtcgg cttcatccga ggaagtcgtg acgccccgga
     6481 gccaggtacc cgataacgaa aagcgtgaac agtataatcc cgaacccgag taccgcgcct
     6541 aaagcagcct tccggccctt ccaacccgag gatagcctcc cgtgtatgag caccgcgtaa
     6601 aggagccagg tgacgagcga ccacgtctcc ttctgccccc agacccacgc ctccccccaa
     6661 atctcctttg cgagtagaag cccgagcgca agccctatgg ttataaacgg gaacccgacg
     6721 atcaggcata cgtggtttat cttgtcgagt gtggtgagag acgggaactt ctgaagcagg
     6781 cttcccacat gtttcgattt tatcctctgt tcctggaaca ggtacatgag gcccgatatg
     6841 aaagccactg tgaataacgc ttccccgagg agaatgagga ctatgtgtat cactatccag
     6901 gggttactga gagatggctc gtgctcgata atccccttag ggaggattac gtgaggcagc
     6961 gtcattatga aggcgagcgg ggagacgaac gcccccagga gaggggcgtt gaatattagc
     7021 tgagacccta tgaacacgat cgtaatcgcg agggcaaaaa tataaagcgt cgtgtcgagc
     7081 ccgcccgtga ggatttgtcc tttcagatag agcgccacga tgctggccgc ctggactacc
     7141 gtgcccagga cgaccgtata gaatcccacc ttcgagcccg tcctgctgtc cgtccagatg
     7201 aagctcatgt agaagaccga cgagatgaag tagagaaggg cggtaaccca gtataaatcc
     7261 atatctatat t
//
LOCUS       DASSJH010000419         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_957457, whole genome shotgun sequence.
ACCESSION   DASSJH010000419 DASSJH010000000
VERSION     DASSJH010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957457"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..713)
                     /locus_tag="VFJ67_09265"
     CDS             complement(54..713)
                     /locus_tag="VFJ67_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7290612.1"
                     /translation="MTRTEILRCYPFYRDADPVFRRELENASVAVKLPPGTDYFREGG
                     QCRQVALIGKGSVRVYKKGETGREITLYYVLPGETCILTASCMLGEKTYPADAVIEEE
                     TEAVILDAGFFRDAVSSREGVRRFVFETFSRRMTEVLTLLEEISFGRMDRRLAEFLSK
                     RLGEAHGDGVLNMTHEQIASELGTAREVVSRLLKEFERTGAVGLSRGRITLRDRSKLE
                     F"
     gene            887..1813
                     /locus_tag="VFJ67_09270"
     CDS             887..1813
                     /locus_tag="VFJ67_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7290613.1"
                     /translation="MNSIISVRNLSKTYASGLRALRNIDLDIKRGEIFALLGPNGAGK
                     TTLISIICGIVNPGGGQVLADGHDIIRDYRAARASIGLVPQELTTDVFETVRDTMNFS
                     RGLFGKPPNPPYIEKVLRDLSLWDKKDTKILALSGGMKRRVLIAKALAHEPRILFLDE
                     PTAGVDVELRRDMWEMVRALRESGVTIILTTHYIEEAELMADRIGIINKGEIILVEEK
                     EALMRKLGKRQLTLYLQNPISSIPAGLNGLTLELSPDGSELVYTFDAHGEHTGIAGLL
                     RLLSEHGIDYRDIHSSESSLEEIFVSLVREKR"
     gene            1810..>2338
                     /locus_tag="VFJ67_09275"
     CDS             1810..>2338
                     /locus_tag="VFJ67_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7290614.1"
                     /translation="MNLYAVRSIYIFELARTWRTLMQSIASPILSTSLYFVVFGAAIG
                     SKVGDIEGVTYGAFIVPGLAMLTIMSESISNASFGIFLPKFTGTIYEVLSAPVSYIEI
                     LIGYVGAAATKSVILGALILATARLFVPYHIEHPLLMLGFLFLTAVSFSLFGFIIGIW
                     ADSFQKLQTVPLMIIT"
BASE COUNT          531 a          679 c          643 g          485 t
ORIGIN      
        1 caggagaaag tttccgtgac taagtcacat atataaaatt tttagtgatt atttcagaat
       61 tcgagcttgc tcctgtccct gagtgttatc cttccgcgcg aaagcccgac cgcgcccgtg
      121 cgctcgaact ctttcaggag cctgctcacg acctcgcgcg cggtgcccag ctcggaggct
      181 atctgctcgt gcgtcatgtt caggaccccg tcgccgtgag cctccccgag ccttttcgag
      241 aggaactccg cgagcctcct gtccattctt ccgaacgata tttcttccaa aagcgtgagc
      301 acttccgtca tgcggcggga gaacgtctcg aagacgaacc tcctcacgcc ctctctcgac
      361 gacaccgcgt ccctgaagaa accggcgtcg agtatgacgg cctcggtctc ttcctcaata
      421 acggcgtctg cggggtaggt cttttcgccc agcatgcagg atgcagtcag gatacaggtc
      481 tcgcccggaa ggacataata gagggttatc tccctcccgg tctctccctt cttgtaaacg
      541 cgcacgctcc ccttccctat caaagccacc tgcctgcact gcccgccctc gcggaaataa
      601 tcggtccccg gagggagctt cacagcgact gaagcgttct caagctctct cctgaagacg
      661 gggtcagcgt ccctgtagaa cggatagcag cgaagtatct cggtcctggt cacgagtcta
      721 gtattacgta cgcgcctgga aaatggaaat atcccgctgg cggagcgtcg tataaattta
      781 atcgcagaca gggcgtgaat cttccgagcg tcacatgaat cttatatatg tatactaaag
      841 cccataaaca gctaaactat acagcttcat aatttatgga gcatccgtga actcgataat
      901 ctcggtcaga aatctaagca agacatacgc gtccggcctc cgggcgctca ggaatatcga
      961 cctcgacata aaaagaggcg agatattcgc gctgctgggg cctaacggcg cggggaagac
     1021 caccctcatc agcatcatat gcggtatagt gaacccgggc gggggccagg tgctggccga
     1081 cgggcacgac ataatacgcg actacagggc ggcccgggca agcatcggcc tcgtccccca
     1141 ggagcttacg acggacgtgt tcgagaccgt acgggacacg atgaacttca gcaggggact
     1201 cttcgggaag ccgccgaacc cgccatacat agaaaaagtg ctccgggacc tctcgctctg
     1261 ggacaaaaag gatacgaaga tactggcgct ctcgggagga atgaagcgga gggtgctcat
     1321 cgccaaggcc ctcgcccacg agccgaggat actcttcctc gacgagccca cggccggggt
     1381 ggacgtcgag ctgagaagag acatgtggga gatggtgagg gcgctccgcg agagcggcgt
     1441 cacgatcatc ctcacgacgc attatataga ggaagctgag ctcatggccg accgcatagg
     1501 gataatcaac aagggagaga tcattcttgt cgaggaaaag gaagctttga tgcggaaatt
     1561 gggcaaaaga caattgacgc tctacctcca gaaccccata agctctatcc ccgccgggct
     1621 taacggcctg acgctcgagc tctcgcctga cgggagcgag ctcgtctata cgttcgacgc
     1681 gcacggcgag catacgggca tcgccgggct cctcaggctc ctgagcgagc acgggatcga
     1741 ttacagggac atacattcga gcgagagctc tctcgaggag atattcgtga gcctggttag
     1801 ggagaaaaga tgaacctcta cgcggtacga tcgatatata tattcgaatt ggcgcgcacc
     1861 tggcgtacgc tcatgcagag catcgcgtcc ccgatactct cgacttcgct atatttcgtc
     1921 gtgttcgggg cggccatagg atcgaaggtg ggggatatcg agggcgttac ctacggcgcg
     1981 ttcatcgtcc ccggcctcgc catgctgacg ataatgagcg agagcatctc gaacgcctcg
     2041 ttcgggatat tcctgcccaa gtttacgggc accatatacg aagtactttc ggctcccgtg
     2101 tcatacatag agatactgat aggatacgta ggcgcggcgg ccacgaaatc ggtcatactc
     2161 ggcgcgctca tactcgctac ggcgcgcctc ttcgtcccgt atcacatcga gcacccgctc
     2221 ttgatgctcg ggttcctctt cctgaccgct gtatcgttca gcctgttcgg tttcataatc
     2281 ggaatatggg cggacagctt ccagaagctc cagacggtgc cgctcatgat aattacgc
//
LOCUS       DASSJH010000420         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1283245, whole genome shotgun sequence.
ACCESSION   DASSJH010000420 DASSJH010000000
VERSION     DASSJH010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283245"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFJ67_09280"
     CDS             complement(<1..899)
                     /locus_tag="VFJ67_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET7290615.1"
                     /translation="MSASGYGNVNFEADSGKAVEDAKLQSALVNVTNRFRQARLRASS
                     EVPGWDELRTLGREIKKEAIENLDRYLLMLEENVKKAGGHVHWAADGEEACKIIIGIA
                     GKNGVKSVVKSKSMATEEVELNRNLEMAGIRAVETDLGEYIIQLAGEHPSHIIAPAVH
                     KTKDDISRLFSEKLGIPYLSDPADLTKVAREMMRSEFLSAGMGVSGANFAVAETGTIV
                     IVENEGNARLTTAVPDIHVALMGIEKIIPRYTDLSLFLTLLARSATGQKSSTYVSFIT
                     GPRRETDRDGPREFHLVLLDNGRS"
     gene            complement(905..1660)
                     /locus_tag="VFJ67_09285"
     CDS             complement(905..1660)
                     /locus_tag="VFJ67_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET7290616.1"
                     /translation="MRVYLFVTCLVDTFFPGVGEGMVKVLDRLGVDMEFPEGQTCCGQ
                     PAFNSGYREDAQAVAERFVSIFGEALKESGDEETYIVCPSGSCATMVKVYYKELLANQ
                     PILLDKLSTITEKTYEFSEFLIKVMKTADVGAGYKGVVTYHDSCHLLRELGVKDEPRR
                     LIKAVNGIEFREMDMHDACCGFGGTFSIKFPRVSVSMLDEKIDCIIGSGADTVVSSDM
                     GCLMNIKGALTRRKIPIKVMHIAELLAHTAGEG"
     gene            1816..>2596
                     /locus_tag="VFJ67_09290"
     CDS             1816..>2596
                     /locus_tag="VFJ67_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase"
                     /protein_id="HET7290617.1"
                     /translation="MKKKELPIPPHFRPGGTGEVFRVEYEKISRVASEWSAAHGLGAS
                     SADTFRVSLIIVDAQNTFCIPGFELFVGGRTGSGAVEDNRRLCEFIYRNLPLITEIAP
                     TMDTHQAMQIFHSVFFVNDLGGHPAPYTLISVEDVEEGRWKFNTRIAGSMGYGEDYIK
                     RHLLHYTKELRRSGKYDLTIWPYHAILGGIGHALVSSVEEAVFFHTIARYTQPDIHVK
                     GDHPLTEHYSVLGPEVSTGPDGKPLTLRTESLFQKPAASESL"
BASE COUNT          522 a          800 c          645 g          629 t
ORIGIN      
        1 gagcgcccgt tgtcgagaag cacgagatgg aattcacggg gcccgtccct gtccgtctct
       61 ctccgcggtc ccgttatgaa cgacacgtat gtcgaggact tctggcctgt ggcgctcctc
      121 gccagaagcg ttagaaaaag cgagaggtcc gtatagcgcg ggattatctt ctcgatgccc
      181 atgagcgcca cgtggatatc gggcactgcg gtcgtgagcc gcgcgttgcc ctcgttctcc
      241 actattacta tcgttcccgt ctcggccacg gcgaagttag cgcccgatac gcccatcccg
      301 gcgctaagga attcgctcct catcatctct ctcgcgacct tagtcaggtc agcggggtcg
      361 ctcaggtaag gtatcccgag cttctccgaa aagagtctcg atatgtcgtc cttcgtcttg
      421 tgaacggccg gggcgattat gtgtgaagga tgctcgcccg cgagctgaat gatgtattcc
      481 cccaggtctg tctctacagc ccttatcccc gccatctcga ggttacggtt aagctcaacc
      541 tcttcagtcg ccatggactt gctcttgacg accgacttca cgccgttctt cccggcgatc
      601 ccaattatta tcttgcacgc ttcctcccca tcagctgccc agtggacgtg tcctcccgct
      661 ttcttcacgt tctcttcgag catgaggagg tatctgtcga ggttctctat agcttccttc
      721 tttatctccc ttccgagcgt cctgagctcg tcccatccgg gcacttcaga gctcgccctc
      781 agcctcgcct gtctgaagcg gttggttacg ttgacgagcg cgctttgcag cttcgcgtcc
      841 tctacggcct tcccggagtc agcctcgaaa tttacgtttc cgtatccgct cgcactcata
      901 ttccctatcc ctctcccgct gtgtgcgcga gaagctccgc tatgtgcatg acctttattg
      961 gtatcttcct ccgcgtaaga gcgcctttta tattcatcag gcagcccatg tccgacgata
     1021 cgaccgtatc cgctccgctc ccgatgatgc agtctatctt ctcgtcgagc atggagacag
     1081 agacccgcgg gaactttatc gagaacgtgc ccccgaaacc gcagcaggca tcgtgcatat
     1141 ccatctccct gaactctatg ccgttcacag ccttaataag cctcctcggc tcgtccttca
     1201 caccgagctc tctcaataaa tgacatgaat cgtggtacgt gaccacgcct ttatacccgg
     1261 cccccacgtc cgccgttttc atcactttta taaggaactc ggagaattcg taggtttttt
     1321 cggtaatcgt actcagctta tctaatagta taggttggtt agctaaaagt tccttataat
     1381 acactttaac catcgtagcg cacgatcccg aagggcacac gatgtacgtt tcctcgtccc
     1441 cgctctcttt aagtgcttcc ccgaatatgg aaacgaacct ctccgcgacc gcctgtgcat
     1501 cctccctgta tccgctgttg aatgcgggct gtccgcagca tgtctgtccc tccgggaatt
     1561 ccatatccac gccgagcctg tcgagcacct tgaccatccc ctcccctacc ccggggaaaa
     1621 acgtatcgac gagacatgtg acgaagagat agactctcat ggcgggattg agcacttcct
     1681 gtcaggtttt ttcttacgga ttcattatcg tccatgattg gtattatttc aattagcggt
     1741 gatcaccgcg atctgttaga ttaatcacga acaggataat tctatgccct gcatgcgcac
     1801 ggccggagct aaacgatgaa gaaaaaagag cttcccatac cccctcactt caggcccggc
     1861 gggacaggcg aagtattccg cgtggagtac gagaagatct cccgcgtcgc ttccgaatgg
     1921 tcggcagcgc acggcctcgg tgcgtcatcc gccgatacgt tcagggtatc tctcatcata
     1981 gtcgatgcgc agaacacgtt ctgcataccg gggttcgagc tcttcgtagg cggacgcacg
     2041 ggaagcggag cggtagagga caaccgcagg ctctgcgagt ttatatatag gaacctccct
     2101 ctcattaccg agatcgcccc gacgatggac acgcatcagg cgatgcagat atttcacagt
     2161 gtattcttcg tgaacgatct gggcgggcac ccggcgccct acactcttat ctccgtcgag
     2221 gacgtcgagg aagggaggtg gaagttcaac acaaggatcg cgggcagtat gggttacggc
     2281 gaggattaca taaagcgcca cctgctccac tacacgaaag agctcaggag gtctgggaag
     2341 tacgacctta cgatatggcc ctaccatgcg atactgggcg gtatcggtca tgcgctcgta
     2401 tcgtctgtcg aggaggcggt tttctttcat acgatcgcgc gctatacaca acccgacata
     2461 cacgtgaagg gcgaccaccc tctgacggag cactattcgg tcctcggccc cgaggtctct
     2521 acagggcccg acggcaagcc cctcacgctc aggacggagt cgctctttca aaagcccgcc
     2581 gcctccgagt ccctct
//
LOCUS       DASSJH010000421         3283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_958448, whole genome shotgun sequence.
ACCESSION   DASSJH010000421 DASSJH010000000
VERSION     DASSJH010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958448"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /gene="ctaD"
                     /locus_tag="VFJ67_09295"
     CDS             complement(<1..1582)
                     /gene="ctaD"
                     /locus_tag="VFJ67_09295"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET7290618.1"
                     /translation="MANHTIAHDVYVPFLEKKGILSWILTTDHKRIGIMYLVSISFFF
                     VVAGIFALLMRYELLTPASDFVQPHTYNVLFTLHGSIMVFFFIVPGLAASFGNFLIPL
                     MIGAPDVAFPKLNLGSYWIYLLGTLIVLIALAKPADTGWTFYTPYSATTQTDVVMITL
                     GVFVLGFSSILTGLNFIVTIHKMRAPGMTWHRLPLFIWASYATAILQLLATPVVGITL
                     LLLIMERTLGVGFFDPAKGGDPVLFQNFFWFYSHPAVYIMIIPAMGVISEIIPVFSRK
                     PIFGYKAIAYSSLGIAFISFLVWAHHMFVAGISETAATIFSFLTFLVAIPTAIKVFNW
                     TATLYKGSIHLHSAMLYALSFIFLFTIGGLTGLFLGALAADVHLHDTYFVVAHMHYVM
                     IGGTVMGFFGGLHFWYAKWFGRMFNETVAKIAWLFIFIGFNVTFFPQFFLGVQGMPRR
                     YATYPAHFESLMSLSTYGSWILAVGMLIMTVNLIYGLFRGEKAPQNPYGSLSLEWQVP
                     SPPPLENFEEIPHVTDWSY"
     gene            complement(1611..2549)
                     /gene="coxB"
                     /locus_tag="VFJ67_09300"
     CDS             complement(1611..2549)
                     /gene="coxB"
                     /locus_tag="VFJ67_09300"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7290619.1"
                     /translation="MTWIPEVASNLAGKVDGVLLLITLMSLVFFILITVVLVYFAIKY
                     RRRREDEETPYITGSEPLEIIWTVIPSILLIVLFVYGYVVYKEMRTPPKDAIDIGVQA
                     KQWLWTFDYYNGKKTINELYVQQNRPVRLVMQSDDVLHDLFIPAFRMKQDVLPGRYVQ
                     QWFTPTKIGTFDIFCAEYCGTGHSAMLAKIVVLSPEAYEIWDKGVKTVEGAGVASLPP
                     AELGEKLYKEKGCNACHSVDGSKIVGPSFKGIYGHTVELQDGSTVDVDENYIRESILE
                     PQTQVVKGFQPVMPSFKGILSDEDITSLIAYIKTLK"
     gene            complement(2575..>3283)
                     /locus_tag="VFJ67_09305"
     CDS             complement(2575..>3283)
                     /locus_tag="VFJ67_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7290620.1"
                     /translation="IDEKTGEFLPGDMRFVDENGKTVELKEFFNKKEPLVLNLVYFGC
                     PRLCNYATEGLLQVMNEEESLDLGKDFKVLTVSFDTKDTPEIAAGKASRYRGMLKHGN
                     PANGNWVFLTSDSGNIHRLTEAVGFKYKVDGDEFAHASALIILTPEGKISRYLPGIQY
                     EPNDFKLSLLEASQGKIGSSAMLNKLLLFCYHFDPIGKKYALQALNVMKAAGVVTLLT
                     LCGVLTYFWRRERQEPE"
BASE COUNT          842 a          869 c          823 g          749 t
ORIGIN      
        1 cgtacgacca gtcggtcaca tgcggtattt cctcgaaatt ctcaagcggc gggggcgagg
       61 gcacctgcca ttcgagagaa agcgagccgt aagggttctg cggcgctttt tctcccctga
      121 aaagaccgta aatcaggttc accgtcatga taagcatgcc gaccgcgagt atccatgagc
      181 cgtatgtgga aagcgacata agcgactcga aatgcgccgg ataagtcgcg taccgtctgg
      241 gcatgccctg cactccgaga aagaactgcg ggaagaacgt gacgttgaag ccgatgaaaa
      301 taaagagcca cgcgatcttc gcgacggtct cgttaaacat cctgccgaac cacttcgcat
      361 accagaaatg gagacctccg aagaaaccca tgaccgtccc gcctatcatt acataatgca
      421 tgtgagctac gacgaaatac gtgtcgtgga gatgcacgtc ggctgccagc gccccgagaa
      481 aaagtcccgt gaggccacct atcgtgaaaa ggaatataaa ggaaagggca tacagcatgg
      541 cggagtggag atgaatggac cctttatata gtgtcgccgt ccagttgaat acttttatcg
      601 ccgtaggtat ggccaccagg aacgtcagga aagagaaaat cgtcgccgca gtctcggaaa
      661 taccggctac gaacatgtgg tgtgcccata cgaggaaact tatgaacgca ataccgagac
      721 tcgaataagc tatagcttta tacccgaata tgggctttct cgagaaaacg gggattatct
      781 ccgaaattac acccatggcc ggaatgatca tgatgtaaac cgcaggatgg gaatagaacc
      841 agaagaagtt ttggaagaga acgggatcac cccccttcgc cggatcgaaa aacccgacgc
      901 cgagggtcct ttccattatc aggagaagaa gcgttattcc gacgacaggg gtcgccagga
      961 gctgtaaaat ggccgtggcg taagaagccc agatgaacag cggaagtctg tgccaggtca
     1021 tgccgggcgc tctcatcttg tggatcgtca ctatgaaatt gaggccggtg aggatcgacg
     1081 agaaaccgag cacgaacacg cccagcgtta tcatcacgac gtccgtctgc gtagtcgcgc
     1141 tatacggcgt atagaacgtc cagccggtat cggccggttt cgccagcgct atgagcacga
     1201 tcagcgtccc cagcagataa atccagtaac tccccaggtt gagcttggga aacgcgacgt
     1261 cgggagcccc tatcattagc ggtatcagga agttgccgaa gctcgcggcc aggccgggga
     1321 ctataaaaaa gaagaccatt atcgatccgt ggagcgtgaa gagcacgtta tacgtatgcg
     1381 gctgtacgaa gtccgaagcg ggggtcagga gctcgtatct catcaggaga gcgaatatgc
     1441 ctgcaacgac gaagaagaac gaaatagata cgagatacat tattcctatg cgtttatggt
     1501 cggtagtcag aatccatgac aatatccctt tcttttcgag gaacggaacg tatacatcgt
     1561 gggcaatcgt atgatttgcc ataaaatcgg cctccttaaa aagctaacat ctattttaac
     1621 gtcttgatat acgcgatgag cgatgtaata tcctcatcgc tcagtatgcc cttaaacgaa
     1681 ggcataacag gctgaaaacc ctttacaacc tgcgtttggg gctcgagtat cgattccctg
     1741 atataatttt cgtcgacatc gaccgtggag ccgtcctgca attctaccgt atggccgtaa
     1801 atgcccttga acgaaggtcc cacaattttg gagccgtcga cgctgtgaca agcattgcat
     1861 cccttctctt tatacagctt ctcacccagc tcggcgggag gcagagaagc gacacccgcg
     1921 ccttcgacag tcttcacgcc cttatcccat atctcgtaag cctcggggct gagcactacg
     1981 atctttgcaa gcatcgccga atgaccggtc ccgcagtact ccgcgcaaaa tatatcgaat
     2041 gttccgatct ttgtcggcgt aaaccactgc tgaacgtatc tacccggcag cacgtcctgc
     2101 ttcatgcgga acgccgggat aaatagatcg tgcagcacgt catcggactg cataacgagc
     2161 ctgaccggcc tgttctgctg cacgtaaagt tcgtttatag tttttttgcc gttgtaatag
     2221 tcgaacgtcc agagccactg tttcgcctga acgcctatat ctattgcgtc cttagggggg
     2281 gtcctcatct ccttataaac cacgtaaccg tatacgaaga gcacgatgag gagtatcgat
     2341 ggtatcacgg tccagattat ttccagcggc tcgcttcccg tgatataggg agtctcctcg
     2401 tcttccctcc ttctcctgta ctttatcgcg aaatacacca gcactactgt gatcagtatg
     2461 aagaatacaa gcgacatcag ggtgataaga agcaataccc cgtcgacctt gcctgccaga
     2521 ttcgatgcta cctcgggaat ccaagtcatt tgtccgtttt aaacctccat tctcctattc
     2581 gggctcctgc ctttctcttc tccagaagta cgtgagtact ccgcaaaggg tcaataacgt
     2641 aaccacaccg gcggctttca tgacgttcaa agcctgaagc gcatatttct tgccaatcgg
     2701 gtcgaaatgg taacagaaaa gaagaagctt gttcaacatt gcggaagagc ctatctttcc
     2761 ctgggacgcc tcgaggagcg agagcttgaa atcgttcggc tcatactgga taccaggcag
     2821 atatctcgaa atctttcctt ccggggtgag tatgatcagc gcggaagcgt gcgcgaactc
     2881 atcgccatcg actttatact tgaaacccac ggcttcggtc agcctgtgta tattgcctga
     2941 atccgaggta agaaaaaccc aattaccgtt tgccggattc ccgtgcttca gcatcccgcg
     3001 atacctggat gccttcccgg ccgcgatctc cggggtgtcc ttagtgtcga agctgacggt
     3061 gaggaccttg aaatccttgc caagatcgag cgattcctcc tcgttcatta cctgaaggag
     3121 tccctcggtc gcgtaattgc acaacctcgg acagccgaag taaacgaggt tcaacacgag
     3181 gggttctttc ttattgaaaa actccttgag ctcgacggtc tttccgttct catcgacgaa
     3241 cctcatatcc ccgggcagga attccccggt cttttcatct atg
//
LOCUS       DASSJH010000422         3780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1508208, whole genome shotgun sequence.
ACCESSION   DASSJH010000422 DASSJH010000000
VERSION     DASSJH010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508208"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFJ67_09310"
     CDS             complement(<1..411)
                     /locus_tag="VFJ67_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7290621.1"
                     /translation="MKILVTGVAGFIGSHLAEKLVKDGYEVRGVDSFLDYYPRPVKEH
                     NLAGLLGNKSFELIESDLLDTDLHKLLDGVEAVFHQAAIAGVRSSWGMRFDEYVRNNI
                     LGTQLLLEASKEMRLKKFIYASSSSVYGDSDELPI"
     gene            539..961
                     /locus_tag="VFJ67_09315"
     CDS             539..961
                     /locus_tag="VFJ67_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290622.1"
                     /translation="MSVIGVASAHAVPPPCSEQELLDSSDYAVEGTVVKIECGKPYDS
                     GECRPAADNAAAFTPETVSKCNATVKVKTNIKGKYAPGDPAPVPFLKVVQACENGSHI
                     IPGSPKADLKENTDIRYYNSGLCRYSNLETLESQEPGR"
     gene            1026..2600
                     /locus_tag="VFJ67_09320"
     CDS             1026..2600
                     /locus_tag="VFJ67_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET7290623.1"
                     /translation="MPLSPFELEIFQNVLSSIAEEMGVVLIRAGFSPNIKERRDLSCA
                     IFKASGEMIAQAAHIPVHLGSMSFAVKAIIGEPGIAEGDVFILNDPFRGGTHLPDVTC
                     IAPVFAEGRLEFFVASRAHHADMGGLTPGSMPLSTTIHEEGTLIPPSRLYRKGRLNRK
                     LFETILSSTRDPEEREGDLRAQTGSLELGLRRLREVIGKYSLKKVREAGDELLGYSEK
                     MMREVIRNIPDGVYRFEDVLDDDGAGTRNIPVRVAVTIKGEKAAVDFRGTSGKVKGCL
                     NTPLSVTTAASVYVFQCLAPEGMPLNSGPLRVIEIIAEEGSLLNAQYPAAVVGGNVET
                     SQRVVDVVFGALSKAVPRKIPAASAGTMSNTTFGGVNPAAGRKFAYYETIAGGMGGRF
                     GKDGVSAVQTHMTNTLNTPIEALERELPVMIDSYSIRERSGGGGRFKGGDGIVRKYRF
                     LTDATVSLITERRRHAPYGIGGGGSGKRGSNTLARKGRAYVIPPKSTFTVRKGDVLEI
                     RTPGGGGWGKPKTHDG"
     gene            2590..>3780
                     /gene="amrB"
                     /locus_tag="VFJ67_09325"
     CDS             2590..>3780
                     /gene="amrB"
                     /locus_tag="VFJ67_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HET7290624.1"
                     /translation="MTDKTTEYPKLRYIEAIPAEIDGEKIICLRDPQNLSDKILAVSI
                     ETLRILSLFDGRRTVRDIQTLIMERTGYLVPSDDIKNLIMQLDEALFLDSRNFEEYKN
                     RIESGFREAGTREPSHAGLSYPGDPRELERWFKAFIGKAEETKPYEEAPGKLRGLISP
                     HIDYVRGGVSYALAYRELLGESDADTFVIFGTSHYAEVENPFILTGKNFLTPLGEAET
                     DRNIIEKLESACNWDLREGEISHRTEHSIEFQVAFLQHMLNGKRKFRIVPILCNSFHR
                     LIKEGRSPSEDEMISVFLEAAAGVVKDMGDRIFIIAGADMAHVGLKFGDDEPVDDTTL
                     GRIRERDMLSLSFGEKMDSEGFYRSVEEEKDWRKVCGLSPIYATLATIDAGRGKLLDY
                     GQALE"
BASE COUNT          980 a         1005 c         1098 g          697 t
ORIGIN      
        1 tatcgggagc tcgtcggaat cgccgtaaac ggaagaggac gaggcgtaaa tgaatttctt
       61 gagacgcatt tccttagagg cctcgaggag gagctgtgtc cccaggatgt tgttcctgac
      121 gtattcgtcg aacctcattc cccagctcga cctcacccct gcgatcgccg cctgatggaa
      181 gaccgcctcc actccgtcga gcagcttatg cagatccgta tctagcagat ctgactcaat
      241 gagctcgaag cttttattcc cgagcaagcc cgcgaggttg tgctccttga ccggacgcgg
      301 ataataatcg agaaacgaat ccactcccct cacctcgtat ccgtctttaa cgagcttctc
      361 cgcgaggtgt gagccaatga acccggcaac ccccgtgaca agaattttca ttcttccgat
      421 tctctcccat tatgcttgat ctcttcgctt tgaatattta gattgacaac gaacgaaagc
      481 acaatacatt agtataatca gggtgaaaca caagttaatt atacctctta ttttaacttt
      541 gtccgtaata ggagtagcga gcgcccacgc agtcccgcca ccctgttccg agcaagagct
      601 cctcgacagc tcggattacg cggtcgaggg gacggttgta aagatcgaat gcgggaaacc
      661 ttacgattcc ggcgagtgca ggccggcggc cgataatgcc gccgctttca cacctgagac
      721 ggtatcgaag tgtaacgcga ctgttaaggt aaaaacgaac atcaaaggga aatacgcgcc
      781 gggagacccg gccccggttc cgtttttaaa agtagttcag gcctgcgaga acgggagtca
      841 catcattccg ggaagtccga aggccgatct caaggagaat accgatatca ggtactacaa
      901 ctccgggctt tgcagatatt ccaatctgga gactctcgag tctcaggagc ccggacggta
      961 agacctaaag cctcttcgaa aattcacccg cgacgctgcc gggaaaagca gcgtgagata
     1021 atcctatgcc gctcagcccg ttcgaactcg aaatatttca gaacgtccta agctcaatcg
     1081 ccgaggaaat gggtgtcgtt ctcatacgcg cggggttctc gcccaacatc aaggagcgga
     1141 gagacctctc ctgcgcgata ttcaaggcct cgggcgaaat gatcgcgcag gctgcgcaca
     1201 ttcccgtaca cctgggctcg atgagcttcg cagtgaaggc gataatcgga gagcccggca
     1261 tcgctgaagg cgacgttttc atactgaacg accctttcag gggagggaca catctgcctg
     1321 acgtcacctg catagccccc gtgttcgccg aaggaaggct cgaattcttc gtcgcctccc
     1381 gcgcacacca cgcggacatg ggcggcctca cgcccggctc gatgccgctc tcgacgacga
     1441 tacacgagga ggggacattg atccctccgt cgaggcttta caggaaaggg agactcaaca
     1501 ggaagctctt cgaaaccata ctctcctcga cacgggaccc ggaggaaagg gaaggagacc
     1561 tgagggccca gacgggctcc ctcgagctcg gcttaaggag gctccgggag gtcatcggca
     1621 agtattcgct gaagaaggtc agggaagcgg gagacgagct tctcgggtac agtgagaaga
     1681 tgatgaggga agtgataaga aacatcccgg acggtgttta caggttcgaa gacgtactcg
     1741 acgacgacgg cgcaggcacg aggaacatac ccgtcagggt agctgtaacc atcaagggag
     1801 aaaaagcggc cgtagacttc cggggcacgt cgggaaaggt gaaggggtgc ctgaacacgc
     1861 ctctcagcgt aaccacggcc gcctcagttt acgtattcca gtgtctcgcg cccgagggca
     1921 tgccgctcaa ctcggggcca ctaagagtaa tcgagattat agccgaagaa ggctcactcc
     1981 ttaacgccca ataccctgcg gcggtggtcg gtggcaacgt cgagacctct cagcgcgtag
     2041 tcgatgttgt attcggtgcg ctctcgaaag ccgtccccag aaaaataccc gccgcgagcg
     2101 cggggacgat gagcaacacc acgttcggag gggtgaaccc agccgcaggc aggaaattcg
     2161 cctattacga aaccatcgca ggcggcatgg gcggaaggtt cgggaaggac ggggtgagcg
     2221 cggtccagac gcacatgacc aacacactca atacacccat agaagcgctc gaaagggagc
     2281 taccggtcat gatcgattca tattcaataa gagaaaggag cgggggcggc ggaaggttca
     2341 aaggcgggga cggcatcgtg aggaaataca ggttcctcac ggacgcgacg gtatcactca
     2401 taacggaacg aagaagacac gccccttacg gcatcggagg cggtggaagc gggaagagag
     2461 gcagcaacac cctcgcgaga aaggggagag cctatgttat tcctcccaag tcaacgttca
     2521 cggtcagaaa aggggacgtg ctcgagataa ggacgcccgg cggaggcggg tggggaaaac
     2581 cgaaaacaca tgacggataa gacaaccgaa taccccaaat tgagatacat agaggcgatc
     2641 ccggccgaga tcgacgggga aaagatcatc tgccttagag acccccaaaa cctctcggac
     2701 aaaatactcg ccgtctcgat agagactctc agaatattga gcctcttcga cggcagaagg
     2761 acagtccgtg acatacagac gctgataatg gagaggacgg gctacctcgt cccttcggac
     2821 gatattaaaa acctgataat gcaattagac gaagctctct ttctcgacag ccggaatttc
     2881 gaagaatata aaaacaggat agagtccgga ttccgtgagg ccggaacgag ggagccgagc
     2941 catgcgggcc tgtcctaccc cggcgacccc agggagcttg aaaggtggtt caaagccttt
     3001 atcggcaagg ccgaggagac gaagccgtat gaagaagcgc cggggaagtt aaggggacta
     3061 atatcccctc acatagatta cgtgagggga ggagtgtcgt acgcgctcgc atacagggag
     3121 ctgctcggag agagcgacgc cgacacgttc gtcatattcg gtacgtctca ttacgcagag
     3181 gtggagaacc cgttcatcct cacaggaaag aacttcctga cgccgctcgg cgaagccgag
     3241 accgacagga atatcatcga gaaactcgaa tccgcctgca actgggattt acgcgagggg
     3301 gagatctcgc acagaaccga gcattcgata gagttccagg tggcgttcct tcagcacatg
     3361 cttaacggga aaaggaaatt caggatcgtg ccgatactct gcaattcgtt ccacagactc
     3421 ataaaagaag gcagatcgcc ctccgaggac gagatgatat ccgttttcct cgaagcggcc
     3481 gcgggagtag tgaaggatat gggcgacagg attttcataa tcgccggggc cgacatggct
     3541 cacgtcgggc tcaagttcgg ggacgacgag cctgtggacg ataccacact cggcaggata
     3601 agggaaaggg acatgctgag cctctcattc ggcgagaaga tggatagtga agggttctac
     3661 aggtctgtcg aggaggagaa ggactggcgc aaggtctgcg ggctttcgcc gatatatgct
     3721 acccttgcta cgattgacgc cgggaggggg aagctccttg actacggaca ggcactcgag
//
LOCUS       DASSJH010000423         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1177704, whole genome shotgun sequence.
ACCESSION   DASSJH010000423 DASSJH010000000
VERSION     DASSJH010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177704"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFJ67_09330"
     CDS             <1..308
                     /locus_tag="VFJ67_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7290625.1"
                     /translation="YKVTPFLGLPVDWVTEITHVKEREYFVDDQRVGPYRLWHHRHDF
                     RETEGGVDVRDTVHYALPLGPLGNIAHELVVKKQLREIFDYRSAYLTQKFGSVKPAD"
     gene            341..1624
                     /locus_tag="VFJ67_09335"
     CDS             341..1624
                     /locus_tag="VFJ67_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290626.1"
                     /translation="MKEKIINISQYKHDQAETDLRMKLVEYSERPEIQSQMGEAFYIW
                     KDDPDFIPDEVTDDQIDELTFEKFFDWFLYDFRLLDTGERIIETFYHDQKGTLSEEEE
                     TILRGWVDAPYSFFEVGDVVHGEYCEIRDLFLNREFRVRDASSSRQLRTSDIIGARPI
                     SAWDNVYFSGIISAYPAAFKNIILDFFESEFRRFRESRGEGADRKEFLRDLGFQISNY
                     ADDLARNPHFITPEGEDLVLASAVYRIGNRDEVLKRLGSIESLTLITRPEDEINIFSL
                     EKSGENDLSGAVEVEENRITIGTYSLQMLGRAKSIIERELNGLIEHLEDRTKGMESYT
                     NRKKENARLGKLPPGVKSRKELDRSLEEYYARWIDLPLPVLNGLTPRDASKTAQGRIN
                     LKTVLDELENFYDHARMRGEPYFDLSRVREELKVK"
     gene            1955..>2589
                     /locus_tag="VFJ67_09340"
     CDS             1955..>2589
                     /locus_tag="VFJ67_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290627.1"
                     /translation="MSRTVKNTLAVIAALFLCFQNLGPAIAQTSLDAQVSLGTMKVSD
                     KVKVTLVDGSEVTLENETYPIYPDMIIDTLDNGKATLIVNGGKYVIEPKSIFKVQSIG
                     DGFTGLLLTQGDEGEVCYCFNPKAGFLVDTPEADTIPPDESAIKAGSPEYVDGRTNVK
                     QNTTSAIQFDGYSDFKTKLATETLEPGDVASSPEKQNPYDSFENKSGCCSPA"
BASE COUNT          722 a          653 c          701 g          513 t
ORIGIN      
        1 cttataaggt cacgcccttt ttaggcctgc cggtggactg ggtaacggag ataactcacg
       61 tgaaggagcg ggaatacttc gtcgacgacc agcgcgtcgg gccgtacagg ttgtggcacc
      121 accgacatga cttcagagag accgaagggg gtgtagatgt cagggacacg gttcattacg
      181 cgctcccgct cggcccattg gggaatatag ctcacgagct ggttgtgaag aagcagctta
      241 gggaaatttt cgattacagg agcgcatatc tcacccagaa gttcgggagt gtgaaacccg
      301 cagattgaca gagacaggag cgaaaatata ctttcctgaa atgaaagaga aaatcataaa
      361 tatatcgcag tataaacacg accaggccga aaccgatctc cgcatgaagc tcgtcgagta
      421 ctcggagcgc ccggagatac agtctcagat gggggaggcc ttttatatat ggaaggacga
      481 tcccgacttc atcccggacg aggtcacgga cgatcagata gacgaactta cattcgagaa
      541 gttcttcgac tggtttcttt acgatttcag gctcctcgat acgggtgaaa ggatcatcga
      601 gacattctat cacgaccaga aggggaccct ctccgaagag gaggaaacca tactcagggg
      661 atgggtcgat gccccgtaca gcttcttcga ggtcggagac gtcgtgcacg gggagtactg
      721 cgagataagg gacttgttcc tcaacaggga gttcagagta cgggacgcct cctcgtcgag
      781 gcagctccgg acctccgata taataggggc gaggcccata agcgcctggg acaacgtcta
      841 tttctcgggg atcatctccg cctatcccgc ggcgttcaag aacataatcc tcgatttctt
      901 cgaaagcgag ttcagacgct tccgggaatc ccgcggagaa ggagccgaca ggaaggagtt
      961 tctgagggac ctcgggttcc agatctcaaa ctacgcggac gatctggcca gaaaccctca
     1021 tttcatcacg cccgagggag aggacctcgt ccttgcgtcc gccgtataca gaatcggcaa
     1081 cagagacgaa gtgctgaaaa gactagggag cattgaatcg ttgaccctca tcaccaggcc
     1141 cgaggacgag ataaatatat tcagtctcga gaaaagcggg gagaacgatc tatcaggcgc
     1201 ggtagaggta gaggaaaaca ggataaccat cgggacatac tccctccaga tgctcggcag
     1261 agcgaaatcc atcatagaaa gggagctcaa tggactcata gaacacctgg aagacagaac
     1321 gaaggggatg gagtcataca cgaacagaaa aaaagaaaac gccagactgg gcaaactgcc
     1381 cccgggggta aaaagcagga aggagcttga caggagcctc gaagaatact acgcacgctg
     1441 gatcgacctt ccgctgcccg tgttaaacgg cctcacacca agggacgctt cgaaaacggc
     1501 tcagggaaga ataaacctca agaccgtgct ggacgagctg gagaactttt acgatcacgc
     1561 acgcatgcgg ggcgagcctt atttcgacct gtcccgggtc agagaagagc tcaaggtgaa
     1621 atgaaccgtc cttgtctcga tttccgggca gggggggatt cgaccatacc ggcgatcctc
     1681 atcgtacctt ccgcggattg cccgggaccc cgggtcagac aatccatccc gcatcgcgac
     1741 cggcatagcc aatatccggg gaagttatca gcaacattaa ataaaatatg tgaggaattt
     1801 caggcggtat gaagcgctta tcatactaaa gtattatgcg aaaaatgccc tcacatactt
     1861 ttttgatacc aaaagaccta cttaagtccc gttgactcac tctaacccca cacctatact
     1921 atattataga tttaggagag gagggtatga gcatatgtcc agaacggtaa aaaatacgct
     1981 agccgtcatt gccgcacttt ttctatgctt tcagaattta ggtccggcaa tcgcgcagac
     2041 atcactggac gcccaggtgt cccttggaac catgaaggtg tccgacaaag tgaaggtcac
     2101 tctggtagac ggcagtgagg ttactctgga gaacgagacc tatccgatct atccggacat
     2161 gattatagat acgctcgaca acggcaaggc cacgctgata gtgaacggcg ggaagtacgt
     2221 catagagccg aaatcaatat ttaaagtaca gtccataggg gacggtttta ccggccttct
     2281 gctgacacag ggagacgagg gggaggtatg ctattgcttc aaccccaagg ccgggttcct
     2341 ggtagatacg cccgaggcgg atactattcc cccggacgag agcgcaataa aggcgggttc
     2401 gcccgaatat gtcgacggca gaacgaacgt taaacagaat acaacttccg ctatccagtt
     2461 cgatggatac tctgatttca agaccaaatt ggccaccgaa actctcgaac ccggcgacgt
     2521 cgcctcaagt cccgagaaac agaaccccta tgattcattc gagaacaaat ccgggtgttg
     2581 ctcgcctgc
//
LOCUS       DASSJH010000424         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1177788, whole genome shotgun sequence.
ACCESSION   DASSJH010000424 DASSJH010000000
VERSION     DASSJH010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177788"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..545)
                     /gene="rimM"
                     /locus_tag="VFJ67_09345"
     CDS             complement(30..545)
                     /gene="rimM"
                     /locus_tag="VFJ67_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7290628.1"
                     /translation="MGLIVYGKITKAHGLSGGLKLLPFSRLPESLNSIKRIFIDIPGK
                     HRPEPFALEECRFEKGSAIIRLEGIDSIEEAGKLTGRDVYIEKSDLPGLEEGEFYWFE
                     LIGLDVYTEDGRYAGRVESLIDRALQSVLVVKDGGKEALIPLSEPIIKEINLKESKII
                     ISPVEGLLSGE"
     gene            complement(563..802)
                     /locus_tag="VFJ67_09350"
     CDS             complement(563..802)
                     /locus_tag="VFJ67_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET7290629.1"
                     /translation="MEKLHELVTFIAKSLVDSPDKVEVREVAGEQTAVLELKVAPEDL
                     GKIIGKQGKTAKAIRTILGAAAAKMKRRAVLEILE"
     gene            complement(879..1112)
                     /gene="rpsP"
                     /locus_tag="VFJ67_09355"
     CDS             complement(879..1112)
                     /gene="rpsP"
                     /locus_tag="VFJ67_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7290630.1"
                     /translation="MVKIRLMRIGSKKNPFYRIVATDSRSPRDGKFLEILGHYDPRKK
                     PHLVEIKADRVKEWISKGAQPTVRVGKLISELN"
     gene            complement(1172..2506)
                     /gene="ffh"
                     /locus_tag="VFJ67_09360"
     CDS             complement(1172..2506)
                     /gene="ffh"
                     /locus_tag="VFJ67_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7290631.1"
                     /translation="MFEGISEKLNTTLKRVRGYGKLSETNIQDALREVRLSLLEADVN
                     FKVVREFVESVKNRALGQEVMASLSPGQQFVKIVHEELIKVLGDESSELDLKASPPVA
                     IMLVGLQGSGKTTSASKLAKYLKETLKRNPYLVPADVYRPAAIDQLKVLAKQVGAGIY
                     DTKAGEKPLEICRNALDEAKLGGYDTLIIDTAGRLHIDEELMDELRTIKRELSPHQVL
                     LVADAMTGQDAVNVSSSFNEALDIDGVVLTKMDGDARGGAALSIKSVTGKPIKFFGTG
                     EKLEALEVFHPDRIASRILGMGDVLTFIEKAQAAFEDKQAEEMAKKLLKKEFSLADFR
                     DAMLAMRKLGSLDSMTQMIPGMKQIANNPRALDMAEKEIKKTIAIVDSMTLAERANHT
                     LLNSSRRKRIAIGSGTRVEDINRMIKNYMQMRNMMKNMGKMGKIAKRMMKFM"
BASE COUNT          531 a          742 c          576 g          794 t
ORIGIN      
        1 cttataactc tggaggcagc gcttgccggt tattctccgg aaagcagtcc ttcgaccggg
       61 gagattatta ttttcgattc cttcaagtta atttccttga ttatcggctc cgagaggggg
      121 atgagtgctt ccttgccgcc gtccttgact acgagcacgc tctggagcgc tctgtctatg
      181 aggctttcta cccttcccgc gtatctgccg tcttccgtgt aaacgtcgag ccctatgagc
      241 tcgaaccagt aaaactcgcc ttcctcgagc ccgggcaagt cggacttttc tatgtacacg
      301 tcccgcccgg tcagtttccc ggcctcttct atggaatcaa ttccctcgag cctgattatc
      361 gccgatcctt tctcgaatct gcactcttcg agcgcgaacg gttcgggtcg atgtttcccg
      421 ggaatatcta taaatattct tttaatcgaa ttgagactct cgggaagacg gctgaacggc
      481 aggagcttca atccgcctga taaaccatgg gccttggtaa tcttgccgta aacgatgagc
      541 cccataaaga cagactagta gtttattcca ggatttccaa tactgccctt cttttcattt
      601 ttgcagcggc cgctccgaga attgtcctta tcgctttcgc tgtcttcccc tgcttcccga
      661 taattttccc caggtcttca ggtgcaacct tcaattccag aacagccgtc tgttctccgg
      721 cgacttcgcg aacttcaacc ttgtcgggac tatcaacgag tgatttagca ataaaagtca
      781 cgagctcgtg tagcttttcc ataacataca cctcccctga attatatcac gttaccggat
      841 ctagccgttt caactccggt accgttcccg tctctattct agttcaactc ggatataagc
      901 ttccctaccc tcacggtcgg ctgcgcccct ttgctgatcc attctttgac cctgtccgct
      961 tttatctcaa cgaggtgcgg tttctttctc ggatcgtaat gacccaaaat ctcgaggaac
     1021 ttaccatccc gcggtgagcg cgaatccgtc gcgacgatcc tgtagaaagg atttttcttc
     1081 gacccaatcc tcataagtct tatcttaacc aaaattctaa tacctcctta tatactctat
     1141 tgtaatgaaa aacgatcaaa taattaagtg gttacataaa cttcatcatt cttttcgcaa
     1201 ttttccccat ttttcccata tttttcatca tatttcgcat ctgcatgtaa ttttttatca
     1261 tccggttaat atcttctacc ctcgtcccgc tcccgattgc gatacgtttc cgcctgctgc
     1321 tgttaagtaa agtgtggtta gccctctccg cgagggtcat cgaatcgact atagctattg
     1381 ttttttttat ctctttttcg gccatatcca gagcgcgcgg gttattggct atctgcttca
     1441 ttcccggaat catctgggtc atgctgtcga gggaccctag tttgcgcata gccagcatgg
     1501 cgtccctgaa atctgcgagc gagaactcct ttttaaggag cttcttcgcc atttcctcgg
     1561 cttgtttgtc ctcgaacgca gcctgggctt tctctatgaa cgtaagcacg tcacccatcc
     1621 cgagtattcg ggacgctatc ctgtccgggt ggaatacctc gagcgcttcg agcttctcgc
     1681 ccgtgccgaa gaactttata ggtttgcctg ttacggactt gatagagagc gccgctccgc
     1741 ccctcgcgtc tccgtccatc ttcgtaagca ctacgccgtc tatgtcgagc gcttcgttga
     1801 agctcgacga aacgttcacc gcgtcctgac ctgtcattgc atcggcaacc aataggacct
     1861 gatgcgggct gagttccctc ttaatggttc tgagctcgtc catgagctct tcgtctatat
     1921 ggagtctgcc ggcggtatcg attattaaag tgtcgtaacc tccgagtttt gcttcatcaa
     1981 gtgcgttcct gcatatttcg agaggctttt ctcccgcctt cgtgtcgtat atcccggctc
     2041 cgacctgctt tgccaggacc ttcaactggt caatggccgc cggtctgtaa acgtcggcgg
     2101 ggacgagata agggtttctt ttcagtgttt ccttcaggta cttcgccagt ttcgaggcgg
     2161 aggtggtttt acccgatccc tggagaccga cgagcattat cgctacaggc ggagacgcct
     2221 tgagatcgag ctccgagctt tcgtcgccga ggacttttat cagctcctcg tgaacgatct
     2281 tcacgaactg ctggccggga gagaggctcg ccataacttc ctggccgagt gcacggtttt
     2341 tgaccgattc aacgaactct ctcaccacct tgaagttcac gtcggcttcg aggaggctaa
     2401 gtcgcacctc tctcaatgcg tcctgtatgt tggtttcgct cagcttgccg tagccacgca
     2461 cccgcttgag cgtggtattt aatttctcgg atattccttc aaacataacc tttagaagat
     2521 acaactcccc cctaaaaagg gtaagccata attaatatca tattcgggct cccttgtcaa
     2581 ttttgattgt tcgtaacttc tcttattgcc ttattatttg gtgtctcggc tgagtttcag
     2641 gcg
//
LOCUS       DASSJH010000425         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_211382, whole genome shotgun sequence.
ACCESSION   DASSJH010000425 DASSJH010000000
VERSION     DASSJH010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211382"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFJ67_09365"
     CDS             <1..557
                     /locus_tag="VFJ67_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7290632.1"
                     /translation="EKNKIHKLGHRSADTAELVFENCEVPEENLLVGEGGFNGAMDTL
                     LGARITHAAKSVGLSQAAFEFALNYSKEREAFGKHISKFQAISFKLAEMAVKIETARL
                     LVYKAAWLYSNDKKALKEASMAKLHAAEVVQWVATEAVQVLGGYGYGVEFPVERYYRD
                     AKLASITEGTSEIQHIVIARELGI"
     gene            complement(682..879)
                     /locus_tag="VFJ67_09370"
     CDS             complement(682..879)
                     /locus_tag="VFJ67_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290633.1"
                     /translation="MAEVTIQIPDPILNIIKKRAEMTKSTPEQLIATTAILCYASAHY
                     DSSKWLKFQDPGKGEMTAPWK"
     gene            complement(901..1929)
                     /locus_tag="VFJ67_09375"
     CDS             complement(901..1929)
                     /locus_tag="VFJ67_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7290634.1"
                     /translation="MSTLISDALPQASQVQPLNTFEAIGLRRSIRWYEPNKPVERWKI
                     QAMLEASRIAPSAGNFNGQRGIVVYRDEDPEIWEFISDWSQITTQMAPILIFWCYDLS
                     AYDVQGQQLHDLMRTGALDKAHGWEYDRVNRLFPLPALLPDFVLHRLACIDLGNAIQN
                     SILTATSLGLGCCLNGASGGARRNVKDKFNLPPSYVFCWLMTVGYPSENLDGGGTRGR
                     PPFETMFFKGKVGQPFERDAKTVKLLEELKMIQTPGPKPGRLEEINKLTIRFGLGKEA
                     LTDWKLTPSQLNDPVNAVDKKVEPLSAAEVEASAAGAKEGDFKLVPTVKREILDQYRK
                     EKGIGDAD"
BASE COUNT          532 a          637 c          642 g          571 t
ORIGIN      
        1 ccgagaagaa caagatacac aagctcgggc acaggtcggc ggacacggcc gagctcgtct
       61 tcgagaactg cgaagtcccg gaagagaacc tgctggtcgg cgaaggcgga ttcaacggcg
      121 caatggacac cctcctcggc gcccgcatca cgcacgcggc caaatcggtc gggctatcgc
      181 aggcggcctt tgagttcgcg ctcaactatt cgaaggagag ggaggcgttt gggaagcaca
      241 tatcgaagtt ccaggcgatc agttttaaat tagcggagat ggccgtgaag atagagaccg
      301 caaggctcct cgtttacaag gcggcctggc tttacagcaa cgacaagaag gccctgaaag
      361 aggcctccat ggcgaagctc cacgcggcag aggtggtgca gtgggtagcg accgaggccg
      421 tccaggtgct gggcggatac ggttacgggg tcgagttccc cgtcgagagg tattacaggg
      481 atgcgaagct cgcctccatc accgaaggaa cctccgagat acagcacata gtcattgcga
      541 gggagctagg catataacgt ccggggattc ctaccgggaa tacgaactga aaaaaaaagc
      601 ggggacgcat cttacgacgc tccccgcttt ttttgtgttc tttattgtaa ccctatctga
      661 ttatgagatc cggcaatgac cttacttcca gggggcagtc atttcgccct tgcccgggtc
      721 ctggaacttg agccacttgg acgaatcata atgagccgac gcgtagcaga gtatagccgt
      781 cgtggctatg agctgctccg gcgtggactt cgtcatttcg gcccttttct ttattatgtt
      841 caatatcgga tcgggtatct gaatcgtaac ttctgccatg atatatgaac ctcctttgat
      901 ttagtcggcg tcgccgatgc ctttttcttt cctgtactga tcgagtatct ctctcttcac
      961 cgtcgggacg agcttgaagt ctccttcctt tgcgcctgcc gccgatgctt cgacctctgc
     1021 cgcgctcagg ggctcgacct tcttgtcgac cgcgtttacc gggtcgttaa gctgtgaggg
     1081 agtgagcttc caatctgtaa gggcttcttt accgaggccg aacctgatcg tgagcttgtt
     1141 gatctcctcg agcctgccgg gcttgggtcc gggcgtctgt atcatcttca gctcttcaag
     1201 gagcttcact gttttggcgt ccctttcgaa gggctgtccg accttgccct tgaagaacat
     1261 ggtctcgaac ggggggcgtc ccctggttcc gccgccgtcg aggttctctg acggataccc
     1321 tactgtcatc agccagcaga atacatatga aggcggaagg ttgaatttat cctttacgtt
     1381 ccttctcgct ccgccgctcg ctccattaag gcagcagccg agccctaaag acgtcgccgt
     1441 gaggatcgag ttctggattg cgtttccgag gtcgatacag gcgagtctgt gaagaacgaa
     1501 atccgggaga agggcaggca gcgggaagag cctgtttaca cggtcgtatt cccatccgtg
     1561 cgccttatcg agggctcccg tcctcataag gtcgtggagc tgctgaccct gtacgtcgta
     1621 cgccgagagg tcgtagcacc agaatataag tatcggagcc atctgcgtcg ttatctggct
     1681 ccagtccgag atgaattccc agatctccgg gtcttcgtct ctgtaaacca ctatacccct
     1741 ctgcccgttg aagttaccgg ctgaaggggc gatccttgaa gcttcaagca tagcctgtat
     1801 cttccatctt tcgacaggct tgttgggttc ataccatctt atcgatctcc tcaagcctat
     1861 cgcttcgaaa gtattaagcg gttggacctg actagcctga gggagagcat cggaaatcaa
     1921 tgtactcatt tcggcctcct tatgtaaatt atgttttctt accagacaat caaccatatt
     1981 attatttccg aaaaaaaaag tcaagctatt cgtgcaataa aggactatta gtactaattt
     2041 tttgcacgag cccgaggggg cggacccatg tacggcggag accgcgcctc actgaggccg
     2101 gcgccctccc cttttccttc tcaaatagtc tcaaattatg taataattca gggttgaaat
     2161 cccggccttg attactatat ttagataacg ataacttaac ggagaaagtg agacaatgaa
     2221 attcgacaaa ctgacaataa aagcacagga agcattggcc gaggcgcagt cgctcgctca
     2281 ggaagggcag aaccaggtgg tggacattcc tcacctcctg acggcgctcc tttcggaggg
     2341 cggcatcccg acacagatac tcgggaagct cggcgtgaac ac
//
LOCUS       DASSJH010000426         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_752712, whole genome shotgun sequence.
ACCESSION   DASSJH010000426 DASSJH010000000
VERSION     DASSJH010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752712"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFJ67_09380"
     CDS             <1..363
                     /locus_tag="VFJ67_09380"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7290635.1"
                     /translation="IAAVFQFVTNIHSVIPMVGASGAISGVLGAYMTFYPRSRILTLV
                     PVFFFIQFIHIPAAVFIFIWFILQFLSGLGTLNAPKESGGIAFWAHIGGFVAGLLLAR
                     FFDRKPYLREASTGGYFH"
     gene            450..1709
                     /gene="pfp"
                     /locus_tag="VFJ67_09385"
     CDS             450..1709
                     /gene="pfp"
                     /locus_tag="VFJ67_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HET7290636.1"
                     /translation="MSGTKKLGRNRLAILVGGGPAPGINGVISAATIEAVNRGFEVIG
                     IREGFRWIADGDTSHIDDLTIRLTSRIHNTGGSIIGISRANPTSSRERMKNTMNSLSK
                     LGVRYLLSIGGDGTMYLASEVEKAGDGRIKVAHVPKTIDNNIPLPGYIPTFGFETARH
                     IGTQLVHYLMEDARTTRRWFFVVTMGRKTGHLALGIGKAAGATITLIPEQFGKGKIAI
                     RDITTILEGSIIKRLADGREDGVAILAEGLTEKLDEEELKNLSDLSRDEMGRIRLSEV
                     DLGELLKHETMKALSEKGLDVRVIDKTIGYELRSAPPIPFDAKYTRDLGYSAVKFLLS
                     GGTGALITLQTGRMVPISFSKIIDPVSNQPRIRYVDIKTESYEVAQKYMIKLSQDDLS
                     DSKKLRALARTANMKPGDFKAYFSRAV"
     gene            1884..>2921
                     /locus_tag="VFJ67_09390"
     CDS             1884..>2921
                     /locus_tag="VFJ67_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET7290637.1"
                     /translation="MFYKYLLPVAGALTLILIGCGAKSPTRSASGPTISGIRLEDTEI
                     DSEIKIVDEDNGEETVDDVRSSFFILNPQVQGWKTYYYIENIFDQFPDVAPETEKEIP
                     VVLNDRVIHYMVYFQNEGRRSFSIWLERSGKYIPLMKEILSQRGMPTDLVYLAMIESG
                     FNVKAESRSAAVGPWQFIKPTALRYGLRVDAWVDERMDPRKSTVAAADYLSDLYAMFQ
                     SWELAAAGYNCGEDRVQAAIDKYQINDFWQISEFTLPTETKNYVPKLMAALIIAKSPD
                     KYGFVGIDYNDPEFYETVMVPPQKSLTEIARVIGVSQYTLVELNPAILLNATPPGGPY
                     QINVPQGYATVT"
BASE COUNT          761 a          752 c          808 g          600 t
ORIGIN      
        1 atcgcggccg tctttcaatt cgttacgaac atccattcgg tcataccgat ggtcggagcg
       61 agcggagcta tctcgggcgt gctcggggcc tatatgactt tttatccgag gtcgaggata
      121 ctcaccctgg tgcccgtttt ctttttcata cagttcatac acattccggc ggctgttttc
      181 atctttatct ggttcatatt acagttcctg agcggtctcg gcacgctgaa cgctccgaaa
      241 gaatcgggag gaatcgcttt ctgggcccat attggaggct ttgtcgcggg gctccttctc
      301 gcgcggttct tcgacagaaa accttatctg cgggaagcgt cgacgggagg gtatttccat
      361 tgaatctata cggccgttta attttcattc gactcgctaa tattatataa tgagccaaac
      421 atccaagcgt acggtaatag gaagagaaga tgtccggaac gaagaagctc ggcagaaata
      481 gactcgcaat actcgtaggg ggaggtcccg ccccgggcat aaacggcgtc atcagcgcag
      541 cgacgatcga ggcggtcaac aggggcttcg aggtgatagg gataagggaa ggcttcagat
      601 ggatagcgga tggcgacaca agtcatatcg atgacctgac catcaggctc acgtcacgca
      661 tacacaacac gggcggctcg atcatcggaa tatcgagggc caaccctact tccagcaggg
      721 agaggatgaa gaatacgatg aacagtctct cgaagctcgg cgtgagatac ctgctgtcga
      781 tagggggcga cggcacgatg tacctggcga gcgaggtgga gaaggccggg gacggcagaa
      841 taaaggtggc gcacgtgccc aagacgatag acaacaatat accgctgccc ggctacatac
      901 ccacgtttgg cttcgagacc gcgcggcata taggaacaca gctcgtccac tacctcatgg
      961 aggatgcaag aaccacgagg cggtggttct tcgtcgtaac tatgggcaga aaaaccgggc
     1021 atctcgccct cggcatcggt aaagccgcgg gggccacgat taccctgata cccgagcagt
     1081 tcggcaaggg caagatcgct atcagggaca tcaccaccat tctggaaggc tccataataa
     1141 aacgtctcgc cgacggcagg gaggacggcg tggctattct cgccgagggg ctgaccgaaa
     1201 agctcgacga ggaagagctc aagaacctta gcgacctgag cagggacgag atgggtcgta
     1261 taagactctc cgaggtcgat ctcggagagc tcctgaagca cgaaacgatg aaggcgctct
     1321 ccgaaaaggg gctcgacgtc agggttatag acaagacaat cggctatgag ctgaggtccg
     1381 cgccgcccat acctttcgac gcgaaataca ctcgcgacct cggttacagc gccgtgaagt
     1441 tcctgttgag cggaggtacc ggagctttga taacacttca gaccgggaga atggtcccga
     1501 tcagcttcag taagatcatc gacccagtat caaaccagcc ccggataagg tatgtcgata
     1561 tcaaaaccga atcgtacgaa gtcgcccaga agtatatgat caagctcagt caggacgacc
     1621 tctcggactc gaagaaactc agggcgctcg ccaggacggc gaacatgaag cccggggatt
     1681 ttaaagctta tttttctcgt gccgtgtaaa aactaaatct cgtttgagaa taaatcggcg
     1741 cagtaaaggc ggcgccggca ttaattcgag agcacctgaa atccggtatt aattagcgtt
     1801 atgtttgatt tttttaagga aggtacttaa atttgcactt caaaacattg acattcatac
     1861 caaattatga taaaaaaaag ccaatgtttt acaagtactt actgcctgtc gcaggtgctc
     1921 tcactctcat tctcataggc tgcggagcca agtcccccac cagaagcgct tccggcccca
     1981 ccatttcggg aattcgtctc gaagatacgg agatcgattc cgagataaaa attgtcgacg
     2041 aggataacgg agaagaaacc gtcgatgacg taaggtcgag cttctttatc ctgaaccctc
     2101 aggtgcaggg gtggaagacc tactattaca tagagaacat attcgaccag ttccccgacg
     2161 tagcgccgga aaccgagaaa gaaattcccg tggtcctgaa cgaccgggtc attcactaca
     2221 tggtctattt ccagaacgaa gggaggcggt cgttcagcat atggctcgaa cgctcgggta
     2281 agtatattcc tctcatgaaa gagatactct cacagagggg gatgccgacc gacctcgtat
     2341 atctcgcgat gatagagagc gggttcaacg taaaggccga atcgaggagc gccgcagtag
     2401 ggccgtggca gttcattaag cccacggcgc tgcggtacgg actcagggtc gacgcatggg
     2461 tggacgagag gatggacccg aggaagtcca ccgtggcagc cgccgactat ctgagcgacc
     2521 tctacgcgat gttccagtcc tgggagctcg ccgccgccgg atataactgc ggggaagacc
     2581 gggtgcaggc agcaatagac aagtatcaga taaacgactt ctggcagata tctgagttca
     2641 cgctccctac ggagaccaag aactacgtcc ccaagctcat ggccgcactg ataatcgcga
     2701 agagccctga taagtacggt ttcgtgggaa tcgactataa cgaccccgaa ttctacgaga
     2761 cggtcatggt gccgcctcaa aagagtctga ccgagatcgc gagagttata ggcgtcagcc
     2821 agtatacgct cgtcgagtta aaccctgcga tcttacttaa cgcgactcct ccgggaggac
     2881 cttaccagat aaacgtgccc cagggttacg cgaccgtgac c
//
LOCUS       DASSJH010000427         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1401273, whole genome shotgun sequence.
ACCESSION   DASSJH010000427 DASSJH010000000
VERSION     DASSJH010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401273"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFJ67_09395"
     CDS             <1..732
                     /locus_tag="VFJ67_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290638.1"
                     /translation="KPASGTPTLDEAWKVFSGYNLNYNKIDETVAMTEEILKREPDNV
                     PALIFLSRVWLTYGYVKARTKEDLIRVFENGKEAAKKAIEIDPRNADAHFFYVANLAS
                     LGDTKGLFNSLFMLPEVRRELETVLELDPDNVNGLAMTGALYLYLPSILGGDLHISEV
                     YLKRSISLNPHVSSAGLYLAVNLKRQKKYDEALEVLKGILDDRKPEFYPDWVENRKYA
                     YAMMRRIEREMEGEKAEKSGGSTLN"
     gene            768..1100
                     /locus_tag="VFJ67_09400"
     CDS             768..1100
                     /locus_tag="VFJ67_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290639.1"
                     /translation="MYIEDLVKLFGTASERRFPALGTDIAREDLRRALESRKYDLQDE
                     AIIEAILKQDSEDLIESFTEAYGSLSDGFKDGEEAVKEAIRLYIASLEHMINYYHTSL
                     IGKHFSST"
     gene            complement(1488..>2651)
                     /locus_tag="VFJ67_09405"
     CDS             complement(1488..>2651)
                     /locus_tag="VFJ67_09405"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7290640.1"
                     /translation="PAFGNTAACISKVTYIDGDNGILEYRGYPIAELAEKSNFLEVAY
                     LLLHGELPTKSEYESWVHNIIHHTIVHENIKRFMDGYRYDAHPMGMLLGTVGALSTFY
                     PEAKDIFNKQIRDIQVHRLIAKMPTMAAFAYRHSLGMPYVYPDNDLSYAGNFLNMLFK
                     MTELKYKPNPAIERAIDILFILHADHEQNCSASAMRNVGSSLPDPYSATAAAIAALYG
                     PLHGGANEGVLQMLREIGSVKNIPEYVKKAKAGEFRLMGFGHRVYKSYDPRAKIIKEI
                     AYDVFEVTGKNPLLDIALELEKIALEDDYFVKRKLYPNVDFYSGLIYQSMGIPMDMFT
                     VLFAIGRTPGWLAQWIELLEDPERRIARPRQVYLGERDRKYVPISERGKKKKA"
BASE COUNT          583 a          701 c          764 g          603 t
ORIGIN      
        1 aagcccgcta gcgggactcc gacgctcgac gaagcctgga aggtattcag cggctataat
       61 ctgaattaca ataaaataga cgagaccgtc gctatgacgg aggagatatt gaagcgggag
      121 ccggacaacg tcccggccct gatattcctc tcccgagtgt ggctcacgta cggatacgtg
      181 aaggcgcgga cgaaggagga cctcatacgc gtcttcgaga acgggaagga agccgcgaaa
      241 aaggccatag agatagaccc ccggaacgct gacgcccatt ttttctacgt cgccaacctc
      301 gcgtccctcg gcgatacgaa gggactattc aattcgctct tcatgctgcc cgaggtgagg
      361 agggagctcg agacggtcct cgagctcgac cccgacaacg tgaacgggct cgccatgacc
      421 ggggccttgt acctctacct gccgtctatt ctcggggggg acctccacat ctcggaggtt
      481 tatttgaaga ggtctatatc gctcaacccc cacgtgtcct ccgccggtct ttacctcgcg
      541 gtcaatctga agagacagaa gaagtacgac gaggctctcg aagtgcttaa gggaatcctc
      601 gacgacagga agcctgaatt ctatcccgac tgggtcgaga acaggaaata cgcttacgct
      661 atgatgaggc gtatcgagcg ggagatggag ggggagaagg cggaaaagag cggcgggagt
      721 acgctcaatt gaaataggga cgaaattgaa ttaggtttgt gagaagaatg tatatagaag
      781 acctggtaaa gcttttcggc accgcctcgg agagaagatt ccccgcgctc gggacggaca
      841 tagcgcgaga ggatctgagg cgcgccctgg agagcagaaa atacgacctc caggacgagg
      901 ccataataga agcgatattg aagcaggaca gcgaggacct gatcgaatcc tttacagaag
      961 cttacggctc cctgtcagac gggtttaaag acggggaaga ggccgtaaag gaggcgatac
     1021 gcctctacat cgcttccctc gagcacatga tcaactacta ccacacgagc ctcataggaa
     1081 agcacttctc cagcacctga cacacactca catgtgtgag ttctatgatt tcttcgtcgc
     1141 tccggaataa aattgatccg gcaggtaaga gcttcatagg aaagcgcttc tccagcacct
     1201 gacacacgtg tgtgtgctct atgatttctc cgtcgctccg gagtaaaaat tgatccggcg
     1261 ggtaagagcc tcataggaaa gcgcttctcg agcacctgac acacactcac atgtgtgagt
     1321 tctatgattt cttcgtcgct ccggaataaa aattgatccg gcaggcatta ccgggctttg
     1381 ttggacattg atcccccatc ctttccttcc ccctggaagg gggaaggaac atgattatgg
     1441 atggagtaaa ttggatattc gttgttgtgg aatcgggcgt gtactaacta cgccttcttc
     1501 tttttgcccc tttcggatat gggcacgtac ttgcggtccc tctctcccag gtaaacctgc
     1561 ctcgggcggg cgattcttct ctcggggtct tccaggagct cgatccactg tgcgagccat
     1621 cccggggtgc gccctatcgc gaacagcact gtgaacatgt ccatcggtat gcccatactc
     1681 tggtagatga gacccgaata gaagtccacg ttcgggtata gcttgcgctt gacgaagtag
     1741 tcgtcctcga gcgcgatctt ctctagctcg agagctatgt cgaggagcgg gttcttgccc
     1801 gtaacctcga agacgtcgta tgcgatctcc tttattatct tggccctggg gtcgtaagat
     1861 ttatagacgc ggtggccgaa gcccatgagc ctgaactcgc ccgcctttgc cttcttgacg
     1921 tattcgggga tgttcttgac cgaaccgatc tctctcagca tctggagcac gccctcgttg
     1981 gcccccccgt ggagcgggcc gtagagcgcc gctatggctg ccgcggtcgc ggagtacggg
     2041 tccgggagcg agcttccgac gttccgcatc gcgcttgcgc tgcagttctg ctcgtggtct
     2101 gcgtggagta tgaatagtat gtctatcgcc ctttctatcg cggggttagg cttgtatttg
     2161 agctccgtca tcttgaagag catattgagg aagttccccg cgtaggagag gtcgttgtcc
     2221 ggatatacgt acggcatgcc cagggaatgg cggtacgcga aggcggccat ggtcggcatc
     2281 ttggctatga gcctgtgaac ctggatgtcc cgtatctgct tgttgaaaat gtccttcgcc
     2341 tccgggtaga aggtcgagag agcgcctacg gtgccgagca gcatccccat ggggtgcgcg
     2401 tcgtagcggt aaccgtccat gaacctcttt atattctcgt gcactatcgt gtgatggatg
     2461 atgttgtgga cccacgattc gtattccgac ttcgtcggca gctcgccgtg caggaggagg
     2521 tacgcgacct cgaggaaatt gctcttctcg gcgagctcgg caataggata gcccctgtat
     2581 tccaggatgc cgttgtcacc gtcgatgtag gtaactttgc ttatgcaggc cgcggtgtta
     2641 ccgaaggccg g
//
LOCUS       DASSJH010000428         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_960165, whole genome shotgun sequence.
ACCESSION   DASSJH010000428 DASSJH010000000
VERSION     DASSJH010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960165"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFJ67_09410"
     CDS             complement(<1..458)
                     /locus_tag="VFJ67_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7290641.1"
                     /translation="MIKRLLPYKDLFFVYVWREFNIRYRQSYIGALWAVIQPLSMMLL
                     FSFVFTYLIKANVSEYPKTVFFYSGLLPWTFFASSVNYSLTSLTGNYLLITKIYFPRE
                     ILPLSGVVTAFLDYVVASVIFIPMLLIYHIHITYYALWMLPLFLLLIVFT"
     gene            complement(495..1400)
                     /locus_tag="VFJ67_09415"
     CDS             complement(495..1400)
                     /locus_tag="VFJ67_09415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7290642.1"
                     /translation="MDIRRGILAAAYKLFPFPLKGDATVDDADPACDLSCVINFYGRV
                     NLLRAILTGLAEQGHDKKRFEVVLVEDRGGTDEGRSISREFEPALDTRYYALEENFGV
                     MGYARNFGLGKTRGKYVLFLDDDTVITDRGFITKLIREFEDGDAEAVIPRGSASYCLV
                     EGRYSFHDPYYPSNRCMAYRRETLRELGGFVDGIIGQEDVEFAVRLTAARKKIRRSHS
                     VSYMHPPLIMGSTNKAAAVGASFARLRNRYPFPVWVMLLVNGSRYIPKLIYPFSRKYR
                     MQGRFSLGFALGILYAITGKKLEYN"
     gene            1480..2004
                     /locus_tag="VFJ67_09420"
     CDS             1480..2004
                     /locus_tag="VFJ67_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET7290643.1"
                     /translation="MGEADRIIISMGDITDMDVDGIVNAANTHLVLGSGVAGSIRRKG
                     GPSIQAECDELGPIPLGEAAVTGGGALKARYVIHAAGMRPGGGVSRESLENTTLNTLK
                     RAEEKGMRSIAFPAIGTGVGGFPIDECAKAMIKTVNEYLKEGGTRIETIYFVLFDEPA
                     WKAFEKELNRVKDG"
     gene            2011..>2246
                     /locus_tag="VFJ67_09425"
     CDS             2011..>2246
                     /locus_tag="VFJ67_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290644.1"
                     /translation="MQFLAQENTIDELEKLLKSRLPVKIEDGGNFVHAAVMMILGESP
                     DGLSMLFIKRPESDVDAFSGHMAFPGGKMKVGDE"
BASE COUNT          532 a          588 c          635 g          491 t
ORIGIN      
        1 atcgtgaaca ctatcagcag caggaacagc ggcagcatcc agagcgcgta ataggtgatg
       61 tgtatgtgat agataagcag cattggtata aatatgacgg acgctacgac gtagtcgagg
      121 aacgccgtca caacgcccga gagcgggagt atctccctcg ggaaatatat cttggtgatg
      181 agcagatagt tcccggtgag gcttgtcagc gagtaattga cggacgacgc gaagaacgtc
      241 cacggcagaa ggcccgaata aaaaaatacg gtcttcgggt attccgacac attggccttg
      301 atgagatacg taaagacgaa cgaaaagagc agcatcatgc tgagcggctg tatgaccgcc
      361 cagagagcgc ctatatagga ttgccggtat cttatattga attcccgcca gacatagacg
      421 aaaaagaggt ctttgtacgg tagcagtctt ttaatcatgg atgtcagtag gcccgtatca
      481 ccccgggccg ggtcttaatt atactccaat ttctttcccg taatcgcgta cagtatgccg
      541 agcgcgaagc cgaggctgaa tctcccctgc atcctgtact tcctgctgaa aggatagatc
      601 agcttcggga tataccttga gccgtttaca aggagcatca cccataccgg gaacggatac
      661 ctgttcctga gccttgcgaa cgacgccccg accgccgccg ccttgttggt gctccccatg
      721 atcagcgggg ggtgcatgta gctgaccgaa tgcgaacgtc tgatcttttt ccgggccgcg
      781 gtgagcctta cagcgaattc gacgtcctcc tgcccgatta tcccgtccac gaaccctccc
      841 agctcccgca gcgtctctct cctgtaagcc atacacctgt tcgaaggata gtagggatcg
      901 tggaagctgt acctgccctc gaccaggcaa tagctcgcgc tcccgcgcgg tataacggcc
      961 tccgcatccc cgtcctcgaa ctcgcgtatc aattttgtga tgaatcccct gtccgtaatc
     1021 acggtgtcgt cgtcgaggaa taggacgtat ttccccctcg tcttgccgag cccgaaattc
     1081 ctggcgtacc ccattacgcc gaaattctct tccagggcgt aatatctcgt gtcgagcgca
     1141 ggctcgaatt ccctggatat gcttcttccc tcatcggtcc cgcccctgtc ctcgacgagc
     1201 acgacctcga atcttttctt atcgtgcccc tgctcggcaa ggcccgtgag tattgccctc
     1261 agaaggttta ccctcccgta gaagttaatg acacacgaca ggtcgcatgc ggggtcggcg
     1321 tcatcaacgg tggcatcgcc cttcaggggg aacggaaata atttatatgc tgcggccaat
     1381 ataccccgtc tgatgtccat tcgattgttg aggctttcga ggttatagat taaattagta
     1441 attttatcgc aataaaagtc aagagtcggg tgcgggacta tgggtgaggc agatcgtatt
     1501 attatctcga tgggcgatat taccgatatg gatgtcgacg gtatcgtgaa tgcggccaac
     1561 actcacctcg tcctcgggag cggtgttgct ggctcgatca ggcggaaggg cggcccgtcg
     1621 atacaggcgg aatgcgacga gctcggcccc ataccccttg gcgaagcggc ggtcacgggt
     1681 ggaggggcgc ttaaagcccg gtacgtgatt cacgccgccg gcatgcggcc cgggggcggg
     1741 gtaagtcgtg agtccctcga aaacacgact ctaaacaccc tcaaaagggc tgaagagaag
     1801 ggaatgcgtt cgatagcgtt tccggcaata ggtacgggcg tggggggatt cccaatcgat
     1861 gaatgcgcga aggcaatgat aaaaacggtc aatgagtatc tgaaagaggg cggtacgcgg
     1921 atcgagacaa tatacttcgt tcttttcgac gagcccgcgt ggaaggcgtt cgaaaaggag
     1981 ttgaatagag taaaggacgg ttaagcaggc atgcagtttc tggcgcagga gaacactatc
     2041 gacgagcttg agaagctctt gaaatcgaga ctcccggtta agatcgagga cgggggaaac
     2101 ttcgtccacg cagctgtgat gatgatactg ggcgagagcc cggacggcct ctccatgctc
     2161 ttcatcaaga ggcccgagag cgatgtagac gctttctccg gacacatggc gtttcccgga
     2221 ggcaaaatga aggtaggaga cgagag
//
LOCUS       DASSJH010000429         6038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1614083, whole genome shotgun sequence.
ACCESSION   DASSJH010000429 DASSJH010000000
VERSION     DASSJH010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614083"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1211)
                     /gene="gmd"
                     /locus_tag="VFJ67_09430"
     CDS             complement(168..1211)
                     /gene="gmd"
                     /locus_tag="VFJ67_09430"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET7290645.1"
                     /translation="MKKALITGITGQDGSYLAEFLMSKGYEIHGLIRRSSTFNTERID
                     HIYVDPHIPGAGLYLHYGDMSDSSNLTNLVYSVRPDEIYHLAAQSHVRVSFEMPEYSS
                     DITALGTVRLLESIRKSGIKCRFYQASSSEMFGASPPPQNEETSFRPMSPYACGKLMA
                     HWTAVNYRDGYGLFTAGGILFNHESPRRGETFVTRKITRAIPEIVLGKQDCLYLGNLD
                     PRRDWGYAPEYVETMWGMLQQDEPRNFVIGTGETHSVREFVEEAFSYAGLDWRKYVKT
                     DPNYFRPNEVESLQADCGKAREALGWEPSVRFRDLVRIMVDADMRKAGLEPAGEGDRI
                     LEAKYPGRWWKAD"
     gene            complement(1344..2330)
                     /locus_tag="VFJ67_09435"
     CDS             complement(1344..2330)
                     /locus_tag="VFJ67_09435"
                     /inference="COORDINATES: protein motif:HMM:NF017630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NodZ"
                     /protein_id="HET7290646.1"
                     /translation="MSVIISSLIFLLRLLKEHRKVIVSCRNSGFGDNLLAAANAWYYA
                     KNTDRALVIYWHNSRYLADKGENAFSHFFDVPASIEGAPIIAGPRLDRLSDFLILHPF
                     YLLPRPDPLMLMYRLTSKLGIRTDYRLMKRISNRQKVTDGIITGLADVSRRIIVTHGC
                     YVPNEHLRPFFDSLALNGEYQKRADAFSDMNFKGKKVIGVHIRYYNQSMQPSDHTKYW
                     LDQPKALSECLDKIQRAAAGLAEPEYVVFLCTDSRKVHDFIIRSVCNVVTYEKAFGRD
                     SSKELHEEIPKETAAATVIEMFLLAKSDVLVRFPPGSWFSHYASLYAKKIVT"
     gene            2458..2922
                     /locus_tag="VFJ67_09440"
     CDS             2458..2922
                     /locus_tag="VFJ67_09440"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7290647.1"
                     /translation="MGSINMPGKYQALSLGGQLYKFFVKGEDTGGAYAFFEARLPPLD
                     IGPPPHIHRNEDVAFYIIEGRFTFSLENSEIKAGPGDFIFLPRGQKHWLRSDSDEVGK
                     MLVFASPAGFEKFYDAAGTPVEDDTKMPPPPTHEELVKLIQEAPNFGIEMFL"
     gene            2958..3413
                     /locus_tag="VFJ67_09445"
     CDS             2958..3413
                     /locus_tag="VFJ67_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7290648.1"
                     /translation="MNPVNKKKSVAVAGSIYKYIATGEDTGGAYSLFEAIVPPKDPGP
                     PPHIHRNEDEAFYVLEGEFSVSLGDEEFRAGRGGFIFLPRGISHSFRSESDEVGRLLV
                     IVNPSGFEKFFDAVGESVEDDTVLPPLPSDEHIRKIIDEAPKFGIQLIL"
     gene            3638..4063
                     /locus_tag="VFJ67_09450"
     CDS             3638..4063
                     /locus_tag="VFJ67_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011625360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7290649.1"
                     /translation="MKKTLEGGCLCGEIRYSVEGDVIDAGYCHCRVCQRASGAPAIAW
                     FTVPVDSFTYTHGKPSAFRSSAQSQREFCGRCGTQIAFRRGEHAVTVDVSIASLDDPS
                     LVEPEYHIWTESRIPWFETRDTLPRHHDSGPDAIGMDEL"
     gene            complement(4172..4552)
                     /locus_tag="VFJ67_09455"
     CDS             complement(4172..4552)
                     /locus_tag="VFJ67_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290650.1"
                     /translation="MGFLSNSDLKQFSCGDVIPGSSGMLTLAFGPAADQLSGAPAGNT
                     QYPFWFGWYRCNMGEELKRWKNSETHVAYFVTKGSFKVLSEGVTKTIGQGTWIGFPPK
                     TEYEVHCMENGSELLWVYDPPKAG"
     gene            complement(4863..5279)
                     /locus_tag="VFJ67_09460"
     CDS             complement(4863..5279)
                     /locus_tag="VFJ67_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290651.1"
                     /translation="MSLKTGEKEIILGFVLENEDNLKLALKVHEVYDDVRKNILSKFF
                     NALEEKLRKHLSDQWVVDNELKNDIYAKNKGLYITKKSWEEIYYIGIGAEKYGAMDFW
                     IGIYREKGSDPLLDGKLKLILDEKYKYGYTTNAVWW"
     gene            complement(5281..5850)
                     /locus_tag="VFJ67_09465"
     CDS             complement(5281..5850)
                     /locus_tag="VFJ67_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7290652.1"
                     /translation="MFDYIYPDENTISDVIANMLDPSGTHGQGEKFLRNFLSEIGISI
                     NSDKVGVQIKREETTKYIRSALRRIDITIDFNNCERAIAIENKPWDGEQLDQVKDYID
                     HLSKKYKDNYVLVYLTGDGSEPQSIEQILKVALIAEGRLRVIPYIPRLMNWLEVCFKD
                     CQSEKMRLFLRDFMRYIENNFRRSFEEGD"
BASE COUNT         1480 a         1608 c         1369 g         1581 t
ORIGIN      
        1 atccttatat tttttctctc gactcccctc gctgctaatt cttcggatac gaacgatccg
       61 agaaacccgc tcccgccggt gagcaatatt tttttcgatg tgaaattctc ggccattgcc
      121 ccctccgatt tctttttaat cccggagacg ccgctcccac gcagccgcta atccgctttc
      181 caccatctgc cgggatattt tgcttccagt atcctgtccc cttcgcccgc cggctcgagc
      241 ccggctttcc tcatatccgc gtcgaccatt atccgcacga gatcacggaa cctcaccgag
      301 ggttcccagc cgagggcttc cctcgctttc ccgcagtcgg cctgcaggct ctccacctcg
      361 ttcggcctga aataattggg atcggtcttc acgtacttcc tccagtcgag ccccgcgtag
      421 gagaaagcct cctcgacgaa ttccctcacc gaatgagtct caccagtccc gatgacgaaa
      481 ttccgcggct cgtcctgctg gagcatcccc cacatggtct ccacgtactc gggcgcatac
      541 ccccagtcgc gcctgggatc gagattgccc aggtaaagac aatcctgttt cccgagcact
      601 atttcgggga tggccctcgt tatcttccgt gtaacgaacg tctctcccct tctcggggat
      661 tcgtggttga acagtatccc gcctgcagtg aagagcccgt acccgtcacg gtaatttaca
      721 gcggtccagt gcgccatcag cttcccgcac gcgtaggggc tcatgggccg aaaactggtc
      781 tcctcattct gaggcggcgg agacgcgccg aacatctcgc tgctggacgc ctgatagaac
      841 cggcatttta ttccgctctt tcttatggat tcgagaagcc tcacggtgcc tagcgctgtg
      901 atgtcgctgc tgtattcggg catctcgaaa ctcacgcgca cgtggctctg cgcggcgaga
      961 tggtatatct cgtcgggtct tacgctgtat acgaggttcg tcagattgct ggaatcgctc
     1021 atatccccgt aatgaaggta tagccctgcg ccgggtatgt gcggatcgac gtaaatgtga
     1081 tcgatcctct ccgtattgaa cgtgctcgat ctcctgatga gcccgtgtat ttcataacct
     1141 ttcgacatca ggaattccgc caggtacgac ccgtcctgac ccgttattcc cgtaataaga
     1201 gcctttttca tggcaaaccc cgtttcattc cgataatgaa aaccatatta taaattaaat
     1261 ttaagccgtt ttacgcaatc ttgtgactga agcctcttcc cggttgaaga cggctccgcg
     1321 cacacgggcc tcaggacggg acgtcacgta acaatttttt tcgcgtacaa gctcgcgtag
     1381 tgagagaacc acgaccccgg cgggaacctc acgagcacgt cgctcttcgc gagcaggaac
     1441 atctcgatca cggtagccgc ggccgtttct ttgggaatct cctcatgcag ctctttcgag
     1501 ctgtccctgc cgaatgcctt ctcatacgtc actacattac agacagaccg gataatgaaa
     1561 tcgtggactt tacgtgaatc ggtgcacaaa aaaaccacgt actccggttc cgcgagcccg
     1621 gccgccgccc tctgaatctt atccagacac tcggagagcg cctttggctg atcgagccag
     1681 tatttcgtat gatcggaggg ctgcatgctt tgattgtaat atcttatatg cacgcctatt
     1741 acttttttcc ccttgaaatt catatcggag aacgcatcgg ctcttttttg gtactccccg
     1801 ttcagagcga gcgaatcgaa aaaaggcctc aggtgctcgt tcggaacgta acatccgtgc
     1861 gtgacgatta tcctccggct cacatccgcg agaccggtga taataccgtc cgtcactttc
     1921 tgacggttac taattctctt catgaggcgg taatccgtgc ggatgcccag cttgctcgtt
     1981 aacctgtaca tgagcatgag cgggtcaggc cgggggagga gatagaaagg atgtaatatg
     2041 agaaagtccg acaaacggtc caaacgaggg ccggcgatta taggcgcgcc ctctatgctc
     2101 gcggggacgt caaagaagtg agagaaggcg ttctcgccct tatcggcgag gtagcgtgaa
     2161 ttatgccagt atatgacaag cgccctgtcc gtgttcttcg cgtaatacca ggcattggcg
     2221 gccgccaaaa ggttgtcgcc gaacccgctg ttgcggcacg aaacaataac cttcctgtgt
     2281 tctttcagga gtctcaggag aaagatcagg gaagatatta tgactgatat gtgttaaatg
     2341 gcacaatatg cggacgacgg catgcgtatt accgaacctt tgtatatgct aaatataaac
     2401 ccttcccaaa accggaataa ttattaaaat catgtttaat tttgatcagg aggagatatg
     2461 ggcagtataa atatgccggg taagtaccag gccctttcgc tcggaggcca gctctacaag
     2521 tttttcgtca agggcgagga taccggaggc gcatatgcat ttttcgaagc gcgccttccg
     2581 cccctcgaca taggcccgcc tccgcacata cacaggaacg aagacgttgc gttctacatc
     2641 atcgaaggcc ggtttacgtt ctcactcgaa aacagcgaga taaaagcagg gccaggggat
     2701 ttcatattcc tgccccgcgg acagaagcac tggctgagga gcgattccga cgaggtagga
     2761 aagatgctcg tcttcgctag ccccgcgggt ttcgaaaagt tttatgacgc ggcggggacg
     2821 cccgtagagg acgatacgaa aatgccgcct cctcccacac atgaagagct cgtgaaatta
     2881 attcaggaag cacccaattt cggcatcgaa atgtttctct gagaagctgc ttcattcgtt
     2941 ataaaaggag aaaaaacttg aacccggtaa ataaaaagaa atcagtggct gtcgcaggct
     3001 cgatatacaa atacatagcc acaggggagg acaccggagg ggcttattct ttgttcgagg
     3061 cgatagtccc gcctaaagac ccgggcccgc ctccgcacat acacaggaac gaggacgagg
     3121 ccttctacgt gctcgagggc gaattctcgg tttctctcgg cgacgaggaa ttccgcgccg
     3181 ggcggggcgg ttttatattc ctccctcgcg ggatcagcca ctcgttcagg tccgaatccg
     3241 acgaagtcgg caggcttctg gttatagtca acccttcagg gttcgagaag ttcttcgacg
     3301 cggtcggaga gtctgtcgag gacgataccg tgctccctcc gctgccttcc gatgagcata
     3361 tacggaagat aatagatgaa gcgccgaagt tcggcataca gctgattctc taggaatgga
     3421 aagccgggag tcttaacccg aattaaattg agacggtgat aaccgcgttg cctgtcccaa
     3481 tttaatttat tgaacgatta caagatatat acacggttgc ccgtatggcc ggaattaatc
     3541 ttaaacagtg cggaagccct cccccacttg acctgaagtc tcatacacat aatactgtga
     3601 agacagaaac tattcctccc ggggtcgagg tcataaaatg aagaaaacac tcgaaggcgg
     3661 atgcctatgc ggtgaaatcc gatacagcgt ggagggggat gtaatagacg cgggctactg
     3721 tcactgccgc gtatgccagc gcgcgagcgg ggcgcccgca atcgcctggt tcacggtgcc
     3781 cgtagattcg ttcacttaca cgcacggcaa gccctcggcg ttccgttcgt cggcacagag
     3841 tcagcgcgag ttttgcggaa gatgcggcac gcagatcgcg ttcaggagag gtgagcacgc
     3901 ggtaacggtc gatgtctcca tcgcgagcct ggacgacccg tcgctcgtcg agcccgaata
     3961 tcacatttgg accgagagcc gcataccgtg gttcgagacg agggacactc ttccgcgcca
     4021 tcacgacagc gggccggacg caatcggaat ggacgagctg tgagcggttt ccccgcacgg
     4081 ctcactcgac aagaaaaaag atccgctgcg ggagcccttc ctctccgcag cggatcaaaa
     4141 caacaggcaa tctgtatgat gtaatccggt actatccggc tttcggcgga tcgtagaccc
     4201 acaggagctc cgagccgttc tccatgcaat gtacttcata ctcggttttg ggcgggaatc
     4261 ctatccacgt cccctgccct atggtcttgg tcacgccttc gctcaggacc ttgaagctcc
     4321 ccttcgtcac gaagtacgcg acatgggttt cggaattttt ccatctcttc aattcttcac
     4381 ccatgttgca tctgtaccag ccgaaccaga acgggtactg agtgttcccc gcaggcgcgc
     4441 cgctcagctg gtcggccgcg gggccgaaag cgagcgtcag catgccgctg ctgcccggaa
     4501 taacgtctcc gcagctgaac tgctttaaat cagagttgct taaaaaaccc atgatatcac
     4561 cccctgtcaa ggattttttt aattacggaa ccgttataac atacgggttc ggacgataca
     4621 acaaaaattt ccactccaga gagatttcat tggcgaaagt ggcgacggga attcgcgaaa
     4681 ggtctagtga cagcgaaaag aatatattac ttcgatttct ttaaagcttc atctatttta
     4741 ttttctgata attttgcaag agctatgaat aaattgaaaa cgtatgtttc tacttcatcc
     4801 gaaaactgca ttttccccaa cagatctata ttattaaaat ctcgataggg ttgatccagc
     4861 cactaccacc acacagcatt ggttgtatat ccatatttgt atttctcatc caaaatcagc
     4921 tttaactttc catctaaaag gggatctgaa cctttttccc tataaatccc aatccaaaaa
     4981 tccatggcac catatttttc agcgccaatt cctatataat aaatctcttc ccatgatttc
     5041 tttgttatat agagaccttt attttttgca taaatatcgt tttttaattc gttgtctacc
     5101 acccattggt cagataagtg ttttcgtaat ttttcctcta acgcattgaa gaatttcgac
     5161 aaaatattct ttcttacatc atcgtaaacc tcatgaactt taagcgccaa cttgagatta
     5221 tcttcattct caagaacaaa tcctaaaatt atttccttct cacctgtttt taagctcatt
     5281 ttaatccccc tcttcaaaac tccgtcgaaa attgttctct atgtatctca tgaaatctct
     5341 taagaacaat ctcattttct cggactgaca atctttgaaa caaacttcca gccaattcat
     5401 taaccttggt atatacggaa tcactcttaa tcttccttca gctattaaag ccacttttag
     5461 aatttgctct atactttgcg gttcacttcc atcacccgta agataaacga ggacgtaatt
     5521 gtctttgtac tttttgctta aatgatctat ataatctttg acttgatcca actgttcccc
     5581 atcccaaggt ttgttttcaa ttgctattgc cctttcacaa ttattaaagt ctattgttat
     5641 atcgattctt cttaatgcac ttcgaatata cttagttgtc tcctcccttt ttatttgcac
     5701 gccaacttta tcagaattta tgcttatccc tatttcggac aagaagttcc taagaaattt
     5761 ttcgccttgc ccgtgtgtac cacttggatc gagcatatta gcaataacat cggatatagt
     5821 gttctcatcg ggatatatat aatcaaacac gctaaaatca ctcgataaat acaaatcagt
     5881 gagcttttta ttctgccgaa aaatatcaag cctatatcga agattgttga agaaattttg
     5941 cagttcttgt cgaatcgaac tcattgaaaa atatcatccg cacactgttg agctagccaa
     6001 acaagaaaat tttctaaatt gttaatggtg cttttata
//
LOCUS       DASSJH010000430         6297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1179007, whole genome shotgun sequence.
ACCESSION   DASSJH010000430 DASSJH010000000
VERSION     DASSJH010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179007"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..325
                     /locus_tag="VFJ67_09470"
     CDS             59..325
                     /locus_tag="VFJ67_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET7290653.1"
                     /translation="MIDKELLDIIVCPETKQDLVLAEPGLIERINLLIGKGELRNRSK
                     QAVTEKIDGGLIRKEDRKYLYPIRDDIPILLIDESIPLEALNNQ"
     gene            complement(361..855)
                     /locus_tag="VFJ67_09475"
     CDS             complement(361..855)
                     /locus_tag="VFJ67_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET7290654.1"
                     /translation="MAATLVLAGTFCLQACEKYERVTDKLDFLKYEYFTCDAVSVTDG
                     ENFVCQTPGMDMEQIRLIGISIPRERKDEAKKFCESVLRRGTLVRIEPDKNQKWDGGN
                     IPAYVFAPGGKMLNVLLVENGYAVPAKSEVNEKYMSLFAGAGKKAATEENETAEKGKQ
                     PWLR"
     gene            complement(916..1767)
                     /locus_tag="VFJ67_09480"
     CDS             complement(916..1767)
                     /locus_tag="VFJ67_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7290655.1"
                     /translation="MGILKEFIDSYFLWRDPMIVGAVSGSVCGFLGVYVVLRRIIFVS
                     AALTQISSFGVALSFYLEGFTLGAMSAFIHPFVLSIVFTSLAAVFFAAKRDFFRISQE
                     GVIGFGFLIASGAVIILGDRITKGAHDIADILFGSAVVVDPRDVYIIPVVALAVLIIH
                     IVFFKDFVFVSFDEETARLFKYPVRTLNTAILITIAVVVAVVTRALGALPVFAFLALP
                     PLTSLLLTERLRLVFVISVIIGTLSATLGYFFSFVLSIPTGASMTVCASVFFILGIVW
                     REVKIRT"
     gene            complement(1790..2527)
                     /locus_tag="VFJ67_09485"
     CDS             complement(1790..2527)
                     /locus_tag="VFJ67_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET7290656.1"
                     /translation="MRSLIRLEEVIIGYENRGILRAISLSVDSGQFWGILGQNGGGKS
                     TLLKTILGLLPPVSGKIESQHGIVFGYVPQNEKFDPIFPVSTEEIVSMGRYSRVTAGK
                     KLGKEDREIVMMSMDKVGIRHLRDRTFRSLSGGEKQRALLARAIAGEPDVLVLDEPTA
                     SVDVKGEAQIMDLVQKIRSDDGLTVLMVSHFLNTVTNYADMIILIDKDSGYFRAGVRS
                     DVLTEENLNKFFGLGPGSGQIKNLREI"
     gene            complement(2615..3598)
                     /locus_tag="VFJ67_09490"
     CDS             complement(2615..3598)
                     /locus_tag="VFJ67_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET7290657.1"
                     /translation="MKLYNKIRRFTILIAFAVVSVFFLTRDAAAKVQIVASLPDFGAI
                     AEEIGGDKVNVVSLAKGYQDPHFVDAKPTYVTKLNRADLLIYNGLDLEIGWLPPLITG
                     SRNSKIASTDAQGRLDASAAIPQILEVPTAKIDRSMGDIHPMGNPHYLLDPRNGVPVA
                     AAITERLKEIDPENSSYYEENYNNFVSRLNSKIKEWDRELAPYKGTEIVSYHKNLEYF
                     SEWAGFKEVGFIEPKPGIPPSPSHVAELINQMKATDVKLVIASNYYPQKTGALIAEKT
                     GTVFLSIPTSVGGEDGIKTYTNLFDTIVGDITSALKTETGKNENSASKTGQ"
     gene            complement(3624..5018)
                     /locus_tag="VFJ67_09495"
     CDS             complement(3624..5018)
                     /locus_tag="VFJ67_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290658.1"
                     /translation="MNFLRTIAVFVVLFFASLPCFQSYSFGQESDDVKSEIKELKQMM
                     EKMQQRITELEKKNRTLEEQVKEKEEPKENTVVKEEAETAPVVTEAPPQQEGFLSKVA
                     QTLNPEISVVSLFAAAWYSLNDPVVFAEVDPQNTGVNIQEIELGFQSVVDPYFRFDSF
                     FPINDEGVEVEEAYGTTLFSLPLNSQFRVGKMRAKFGRINQIHRHAQNFVTLPIVAGE
                     FLGEHLNPTSIEANFLVPVPWFLELSASGGSPDVETPTFQRSPDANNVGNLLYIFHLA
                     NFFEVSDSLGVNLGASFATGSNDSGPDERSNLYGVDFFAKYRPLRNNSYQQVSVQSEF
                     MWRQAETPEEELEDYGFYAEGIYRFAKRWNTGLRFDLTDTNTPVTSDDNSEELASADS
                     TDLASTRTINPGETLGLQGRAYRISPMLTFSPSEFSQVRLEYDYLNQNYGPNQHAVFL
                     QFMYAIGAHGAHPY"
     gene            complement(5568..>6297)
                     /locus_tag="VFJ67_09500"
     CDS             complement(5568..>6297)
                     /locus_tag="VFJ67_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HET7290659.1"
                     /translation="GKSTLLSLLLGLLKPAAGEIEVLGVDPSKDYEKIRERIGALLQN
                     VDLQIIAPRVWDDVSFSPRNYGFKEEEISGLVDDILKKLEIEHLKDKVPHYLSGGEKT
                     KVGLAGALVTKPELLILDEPFEHIDPACRTELIKLLNTVNRENGTAIILSTHNMNTVP
                     LIADTVYLIAEGGTIAGKGTPKEVFSEVGTLSKCHIEPPVLGELFAELRKQGVEMDVS
                     LTVDEAACTIANAVVLSKNKTGHG"
BASE COUNT         1380 a         1736 c         1564 g         1617 t
ORIGIN      
        1 gttagtatac ttacaataaa aatttcatcc ggcgagacaa agctttccat atttacctat
       61 gatagataaa gaattgctcg atattattgt ttgtcccgag acgaagcagg accttgttct
      121 tgcggagccc ggtttaattg aaagaataaa cctgctcatc gggaagggag agctccggaa
      181 caggtcgaaa caggctgtaa ccgagaagat cgacggcggt cttataagga aggaagaccg
      241 caaatacctc tatccgataa gggatgatat ccccatacta cttatcgacg aatcgatacc
      301 tctggaagcg ctcaataacc agtaaccgtt tccgtttctc attatatgga gacggtgttt
      361 ttacctcagc cagggctgct tccctttttc cgccgtttcg ttttcctctg tcgctgcctt
      421 cttccctgct ccggcgaaga ggctcatgta tttctcgtta acctcgcttt tggccggcac
      481 tgcgtatcca ttttccacaa gaagcacgtt aagcatcttc cctcctggtg cgaatacgta
      541 agccggaatg ttccctccat cccatttctg attcttgtcg ggctcgatcc tgacgagcgt
      601 ccctcttctc agtaccgatt cacagaattt ctttgcctcg tcttttcttt ctctggggat
      661 gctaatccct atcagcctga tttgttccat gtccattcct ggcgtctgac agacgaagtt
      721 ttccccgtcc gtgacgctca cggcatcgca tgtgaaatac tcgtatttaa ggaaatccag
      781 cttgtctgtg acccgctcat acttttcgca tgcctgcaag cagaaagtcc ccgccaggac
      841 gagagtcgcc gccattaacc gatgtattat tgtcttccgt gaaaataaat tcatcttcat
      901 cgccgcttca gttgattatg tcctgatttt tacctcgcgc catacgattc ccaggatgaa
      961 aaatacggaa gcgcagacgg tcatggacgc tccagtcggt atggacaata cgaacgagaa
     1021 gaagtatccg agcgtcgccg agagagtacc tataatgacg gaaatgacga aaaccagtct
     1081 gagcctttcc gttagaagaa gggacgtgag cggcgggagc gcgaggaacg cgaatacggg
     1141 tagggcgccc aaagccctcg tcacgactgc aaccacgacc gcgatcgtaa tcagaatggc
     1201 ggtgttgagg gttctgaccg ggtacttgaa cagccgcgcc gtttcttcgt cgaaggagac
     1261 gaagacgaag tctttgaaga aaacaatatg tattatcagg accgcgagag ctaccaccgg
     1321 gattatgtac acgtccctcg gatcgactac gaccgcgctc ccgaacaata tgtccgctat
     1381 gtcgtgcgcc cccttcgtaa tgcggtctcc gaggataatt accgcgcccg aagcgattaa
     1441 aaatccgaac cctataaccc cttcctggct gattcggaag aaatccctct tcgccgcaaa
     1501 aaatacggcc gccagagagg taaacacgat cgagagaacg aacggatgta tgaatgccga
     1561 catcgctccc agggtaaaac cctcgagata gaacgatagt gccaccccga agctcgatat
     1621 ctgcgtgagc gccgcgctta cgaatattat ccgcctgagg acgacgtaaa cgccaagaaa
     1681 tccgcagacc gatcccgaca cggcgcctac gatcatcggg tcgcgccaga gaaaataact
     1741 atcgataaac tcctttaaaa ttcccatcgg ctatgccata ttcccatatc tagatttccc
     1801 tcaaattttt tatctgcccg gaacccgggc cgaggccgaa gaatttattc aggttctcct
     1861 cggtcagaac gtcgcttctc acgccggccc tgaaatatcc gctgtctttg tcgatcagta
     1921 tgatcatgtc ggcgtaattg gtaaccgtgt tcaggaagtg gctcaccatc agcaccgtga
     1981 gcccgtcgtc ggaccttatc ttttggacga gatccatgat ctgcgcctcg cccttcacgt
     2041 caaccgatgc cgtgggctcg tcgaggacga gcacgtccgg ctcgccggct atcgccctcg
     2101 caaggagcgc cctctgtttc tccccccctg aaagcgacct gaacgtcctg tccctcagat
     2161 gacggatgcc gactttatcc atggacatca ttacgatctc cctgtcctct tttccgagct
     2221 ttttccccgc cgtaaccctg ctgtacctgc ccattgacac tatctcttcc gttgaaaccg
     2281 ggaatatagg gtcgaacttc tcgttttgag gcacgtatcc gaaaactatc ccgtgctgcg
     2341 attcgatctt tcccgacacg ggcggaagca ggcccagtat ggtcttgaga agcgtagact
     2401 ttcccccgcc gttctgcccc agaatgcccc agaactggcc gctatccacc gagaggctga
     2461 tagctctcaa tatgccacgg ttctcgtaac cgatgatcac ctcttcgagc cgtatcaagc
     2521 ttctcatcag attgtcattt ctccgttttt aaaatgtagt cccccgcgtc tttttctggg
     2581 gaaagaacgg atgggactac gtataaatgc gtcatcattg acccgtcttc gatgccgaat
     2641 tttcattttt tcccgtttct gtttttaacg cggacgttat gtcccctacg atcgtgtcga
     2701 acaggttcgt gtacgtcttg ataccgtcct ctccgcccac gctcgtcggt atggacagaa
     2761 acacagtccc ggtcttctcg gctataagcg ccccggtttt ctgcgggtaa tagtttgaag
     2821 cgataaccag cttcacgtcc gtcgctttca tctgattgat gagctcggcc acgtgagacg
     2881 gcgacggcgg aatgcccggc ttgggctcta taaaaccgac ctctttaaac cccgcccatt
     2941 cggaaaagta ttcgaggttc ttgtgatagg aaacgatttc agtcccctta tacggcgcca
     3001 gctcccggtc ccattcctta attttactat tcagacggct gacgaaatta ttgtaattct
     3061 cctcgtagta ggaggagttc tcgggatcga tctccttcag tctctccgtt atggccgcag
     3121 ctacgggaac gccgttcctg gggtcgagga ggtaatgcgg gtttcccatc ggatgtatgt
     3181 ctcccattga cctgtctatc ttggcggtcg gcacctcgag tatctgaggt atggctgcgg
     3241 aagcgtcgag ccgcccctgt gcgtcggtgg acgcgatttt cgaattcctc gatccggtga
     3301 tcaatggagg gagccagcct atctcgaggt cgagcccgtt gtagatgagc aggtccgccc
     3361 tgttgagctt ggttacatac gtaggcttgg cgtccacgaa gtgagggtcc tggtatccct
     3421 tcgcgagact cacaacgttg accttgtctc ctccgatctc ttcagctatt gcgccgaagt
     3481 ctggaagcga tgccactatc tgcactttgg ccgccgcatc ccgcgtgagg aaaaaaacag
     3541 agacgacggc gaaagcgatc aatatcgtga atcggcgtat cttgttatag agtttcataa
     3601 ttgtttctcc tgagtgaatt tgattaatac gggtgcgccc cgtgcgcccc gatcgcgtac
     3661 atgaattgaa ggaatacggc gtgctgattt ggcccatagt tctggttcag ataatcgtat
     3721 tcgagcctta cctgtgaaaa ttcgctcggg ctgaatgtca gcatgggcga tatgcgatat
     3781 gccctgccct gtaatcccaa tgtctcaccc ggattgatcg ttctggtgct tgcaaggtca
     3841 gtagagtctg cggatgcaag ttcctcgctg ttatcatcgc tggtgacagg agtattcgta
     3901 tccgtcaggt cgaaccttaa acctgtattc cacctcttcg cgaacctgta gataccctcg
     3961 gcataaaagc cgtaatcctc gagctcttcc tcgggcgttt cggcctgcct ccacatgaac
     4021 tccgactgaa ccgatacttg ctgataagag ttattcctga gagggcgata ctttgcgaaa
     4081 aagtcgacgc cgtacaggtt cgacctttca tcgggaccgg aatcgttgct gcctgtggca
     4141 aacgaagcgc ctaggttcac gcccagtgaa tcgctcacct cgaagaagtt agccaggtgg
     4201 aatatgtaga gcaggttgcc tacgttgtta gcgtccgggc tcctctggaa ggtgggagtt
     4261 tccacgtcgg gacttcctcc cgaagcgctg agctccaaaa accagggaac ggggacaagg
     4321 aaattcgcct ctatgctcgt cgggttcagg tgctccccaa ggaattcccc ggcaactatc
     4381 ggcagtgtca cgaagttctg agcgtgtctg tgtatctgat tgatccttcc gaatttcgcc
     4441 ctcatctttc ctacccggaa ttgggagttt aaaggaagac tgaaaagggt cgttccgtat
     4501 gcttcttcaa cctcgacccc ttcgtcgtta atcgggaaaa agctgtcgaa tcggaaataa
     4561 gggtcgacta cgctctggaa ccccagctcg atttcctgta tgttgacgcc tgtgttctgg
     4621 ggatcaactt ctgcaaagac gaccgggtca ttcagtgaat accaggccgc tgcaaaaaga
     4681 ctgacaacgc tgatttccgg gttaagcgtt tgagccacct tgcttaaaaa accctcctgc
     4741 tggggcggtg cctctgtcac aaccggcgca gtctcagcct cctctttcac gactgtgttt
     4801 tctttcggct cttctttttc tttcacctgc tcctcgagcg tcctgttctt tttctcgagc
     4861 tcggtgatcc tctgctgcat cttttccatc atctgtttaa gctcttttat ctcactcttc
     4921 acgtcatcgc tctcctgccc gaatgagtaa ctctgaaaac acggcagcga agcgaaaaac
     4981 agaacgacaa ataccgcaat agtccttaaa aaattcataa ctcacctccg aaaattaaaa
     5041 tgagtgatcg tagcgggttc gatatcgccg tctcggagga gaagcactat ggggccggga
     5101 agatttggct cctgtatgtt ttcaggcgcc tcagcgattt tcccgaaccg tgattgcggg
     5161 ccgggcctag aggcttgatg aaatcgatat gttgtgggtt ttgctccctc cgcctgaacg
     5221 gcgtttatcg gctcagatcg ccggagggga gcgggaaggc ttgttattta ataaaaataa
     5281 atgaggtgtt aagtgttcgt tttctttata tcggacttcc tgcccgggat caacggggct
     5341 gaacacggga ccgggcgaag cgagcttgat attcccatgg agccgacacg cggagcacat
     5401 ttcgactgaa tgacccgctt cctgcgacgc gcccgtaatc tttgcaagcg agtccgggat
     5461 aaagctgatc ttgtggctgt gaatgctgac ggagaaaatg aagagcacgc ttaaaagcgg
     5521 catgaggtac ttcgatgctc ttgaccggcg catagctttg agaggtttca tccgtgaccc
     5581 gtcttgtttt tcgataatac cactgcgttc gctatagtac aagccgcttc atccacagtt
     5641 agggagacgt ccatctcgac cccctgtttc ctgagctcgg cgaacagctc gcccagcacc
     5701 ggaggctcga tgtggcattt ggaaagggtc ccgacctcgg agaagacctc cttgggcgtc
     5761 cctttccccg ctatcgtgcc cccttcggcg attaaataca cagtgtcggc gatgagcggg
     5821 accgtgttca tgttgtgggt cgataaaatt atcgcagtgc cgttttccct gttcacggtg
     5881 ttaaggagct tgatgagctc ggtcctgcag gcggggtcta tgtgctcgaa cggctcgtcc
     5941 agtatgagga gctccggttt ggtaacgagc gcgcctgcta aaccgacctt cgttttttca
     6001 ccgccgctca ggtaatgggg cactttatcc ttcaggtgct cgatctcgag ctttttcagt
     6061 atatcgtcca cgagcccgga gatctcttct tccttgaatc cgtagttcct cggagagaac
     6121 gatacgtcgt cccagaccct gggcgctatt atctggaggt ccacgttctg gagcagcgcc
     6181 cctatcctct ctcttatctt ctcgtaatcc ttcgaggggt cgacccccag cacctcgatc
     6241 tcgcccgcgg ccggtttcag gagcccgagc aagagggaga gaagcgtgga cttcccg
//
LOCUS       DASSJH010000431         4005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1510550, whole genome shotgun sequence.
ACCESSION   DASSJH010000431 DASSJH010000000
VERSION     DASSJH010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510550"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3648
                     /locus_tag="VFJ67_09505"
     CDS             <1..3648
                     /locus_tag="VFJ67_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7290660.1"
                     /translation="REFFINVSEGAVDLFPAVLILAGVLILLRNKTDEDFEKRTGRYL
                     IASSVILGISLGIIYFLAESGPKSGTPNAHTLLTHMWGLTPMVSVIAAAFAAEYYTSR
                     SRTVLSYVLEITLIPLLASEVFVTLVSSLFPGEHFLYADFLKVLACFIPFIGLTLEYS
                     RNYGEKKYALRKVRDAESRLESAAGQSRAEVERLEGRLKELGESEEFREALSAHAPYG
                     TYVVTDGRLVYVNPRFEIITGYTGKELVGSKMDALVSPEDREGHGEYIRGITEGASEE
                     TFKYRIINKKRQAVWVSGTSSKIMYMGRESILGNVVDITSSRHVEEMLRTLSASSPLG
                     IYIVQDGEIKYVNRQFCEYTGYGEEELIGTSAENLVLYLDRETVKEMSNRMLAEKGTQ
                     PYEYRISSKTGEIRWLTETVTRIRYRDRNAVLGSVADITERRRVEMMLRTLSTSSPIS
                     IYIAQDGEFKFVNPQFQNFTGYSEEELLGTKSASYVYEEDRSSVRQYAIRMLKGEVNA
                     PYEYRALRKDGTIIWSMEVVKSIQYMGREAVLGTLMDVSEKKQAEELFQTLSTSTPIG
                     VFIVQDSRFEFVNPQFEEFMGYGKNELLGSNPWKLVHPLDRDEVIEKSKQMQDGTRKL
                     PYEYRVVNGNGETKWVMETSTSIRYRGRQAILGSLMDISEIKRAEEELKGAKEAAEAA
                     SQAKSEFLANVSHEIRTPLNAIVGMTELMLDTSLSSEQQETLRVIESSSDALLSLIND
                     ILDFSRIEAGQMEIEEEAFSLKDLVEGVADSLSVRAFEKGLELVCYINHNVPDRLIGD
                     MSRIRQVLVNLVVNAIKFTDAGEVFINIENMRPVDGDDVEIQFKVADTGIGIPEEHRE
                     RIFEKFSQVDSSTTRTFGGAGLGLSISKSLVDLMKGKIWYESELGKGSTFYFSLPLTL
                     DWEKEGRVARVQPNFRGVPVLVVDDNGTNRSVLRKMLSLWGFEVMESSVGRAALSLLH
                     NMDKKPPLIIVDKQMPGMDGIEFTEHVRNSIGSGIKILLLTSWGGMSFTDIKDRGVDE
                     TIVKPVKSTKLYEAVSRLVREDSYLGVGEQDRHGSSPFESARARKRATPRILVVDDTP
                     DNQNLAKRILEIGGYVVDVASGGKEGVDAACGNIYDLILMDIQMPVVDGFDATKAIRS
                     WEKENRADRTPIVAVTAHALKWYREKCIENDMDDYITKPLTKKSLLDMARKWVSAESA
                     ESGSPGVDVGA"
     gene            3763..>4005
                     /locus_tag="VFJ67_09510"
     CDS             3763..>4005
                     /locus_tag="VFJ67_09510"
                     /inference="COORDINATES: protein motif:HMM:NF025499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HET7290661.1"
                     /translation="MSRNQPELDPDIVGEIRARGIEPGLVLSQIEMFERGMPPAHLVG
                     PCTPGDGIVTIWGEAESYARLYESGSARREIVKFVPA"
BASE COUNT          978 a         1011 c         1266 g          750 t
ORIGIN      
        1 agggaatttt tcataaacgt gtctgagggt gcggttgacc tcttcccggc ggtgctgata
       61 ctcgcgggcg tgctcattct tctcagaaac aaaacggacg aagacttcga aaaaaggacc
      121 gggcgctatc tcatcgcgag ctcggtaata ctggggatat cgctagggat aatatatttc
      181 ctcgcggaat cgggaccgaa atccggcact cccaacgcgc acacgctttt gactcacatg
      241 tgggggctca ccccgatggt ttcggtaata gcggccgcgt tcgcggctga atattatacg
      301 agccggagca ggacggtcct ctcgtacgtc cttgaaataa cgcttatccc tttactcgcc
      361 tccgaggtat tcgttacgct cgtctcgtcg ctttttccgg gagagcattt cctttacgcc
      421 gattttctca aggtgctcgc atgcttcatt ccgttcatag gtctaacgct cgaatactcg
      481 aggaactacg gggagaaaaa atacgctctc aggaaggtga gggatgcgga atccaggctc
      541 gagagcgcgg cgggacagag ccgcgccgaa gtggagaggc tagagggcag gttgaaggag
      601 ctcggggaat cggaagaatt ccgggaagcg ctttccgcgc acgcgcctta cggcacgtac
      661 gtcgttactg acggacggct cgtttacgtg aaccccaggt tcgagatcat caccggctac
      721 accgggaagg agctcgtcgg ctccaaaatg gacgccctcg tgtcccccga agacagggaa
      781 gggcacgggg agtatatccg cggaataacc gaaggcgcat ccgaagagac ctttaagtac
      841 agaatcatca acaagaagag acaggcggtc tgggtctccg ggacttcgtc gaaaatcatg
      901 tatatgggca gggagtcgat tctcggtaac gtcgtcgata tcacctcctc aagacacgtg
      961 gaagagatgc tcaggacact gtcagcgagc tccccgctcg gcatatacat agtgcaggac
     1021 ggcgagataa agtacgtgaa caggcagttc tgcgagtaca cgggatacgg ggaggaggag
     1081 ctcataggga catccgcgga aaatctggtt ctctacctcg accgtgagac ggtgaaggag
     1141 atgtcgaatc gtatgctcgc cgagaaaggt acacagccct acgagtacag gatatcgagc
     1201 aagacgggcg agatcaggtg gctcaccgag accgttacga ggatcaggta ccgcgacagg
     1261 aacgccgtgc tcgggagtgt cgccgatata accgaaaggc ggagggtgga gatgatgctg
     1321 aggacactct ccacgagctc tccgatcagt atatacatag cgcaggacgg agaattcaag
     1381 ttcgtcaacc ctcagttcca gaattttacg ggttacagcg aggaggagct gctcgggacc
     1441 aaatccgcct cctacgttta tgaggaggac agatcgtccg tcaggcagta cgcgataagg
     1501 atgctcaagg gcgaggtaaa cgctccctac gaatacaggg cattaaggaa agacgggacc
     1561 attatttggt cgatggaggt cgtgaagtcc atacagtaca tgggaaggga agccgtgctc
     1621 ggcacgctga tggacgtatc cgaaaagaag caggccgagg agctgttcca gacactttcg
     1681 acctctactc cgataggcgt tttcatcgtg caggattcga ggttcgaatt cgtgaacccg
     1741 cagttcgagg agttcatggg ctacggcaaa aacgagctcc tgggttcgaa cccgtggaag
     1801 ctcgtgcacc cgctcgaccg cgacgaagtc atcgagaagt cgaaacagat gcaggacggg
     1861 acgaggaagc tgccctacga atacagggtg gtgaacggaa atggcgagac gaaatgggtg
     1921 atggaaacga gcacctcgat ccggtacagg ggcaggcagg cgatactagg cagcctgatg
     1981 gacatatccg aaatcaagcg ggccgaggag gagctcaagg gcgcgaagga agcggcggag
     2041 gcggcgagcc aggcgaagtc ggagttcctc gctaacgtga gccacgagat aaggacgccg
     2101 ctcaacgcga tagtcggcat gacggagctt atgctcgaca catcgttatc gagcgagcag
     2161 caggaaactc tcagggtgat agagtcgtcc tcggacgcgc tgctcagcct gataaacgat
     2221 atactcgact tctccaggat cgaggctggt cagatggaga tcgaggaaga ggcgttcagc
     2281 cttaaggatc tggtggaggg cgtcgccgat tccctttcgg tgagggcttt cgagaaaggg
     2341 cttgagctcg tgtgctacat caaccacaac gtgcccgacc gcctgatcgg agacatgtcg
     2401 aggatacggc aggtgctcgt gaacctcgtc gtgaacgcga taaagtttac ggacgcgggc
     2461 gaggtgttca tcaatatcga gaacatgagg cccgtcgacg gggacgacgt cgagatacag
     2521 ttcaaggtcg cggacaccgg catcggtatc ccggaggagc acagggaacg gatattcgag
     2581 aaattttcgc aggtggacag ctccacaacg aggacgttcg gcggagcggg gctgggactc
     2641 agcatatcca agtcgctcgt cgacctcatg aaaggaaaga tctggtatga gagcgagctc
     2701 ggcaaaggtt cgaccttcta cttcagtctc cccctgacgc tcgactggga gaaagaaggc
     2761 agggtcgccc gggtgcagcc gaatttcagg ggcgttcctg tgctcgtcgt ggacgacaac
     2821 gggacgaaca ggtccgtgct caggaagatg ctctcgctat ggggcttcga ggtgatggaa
     2881 tcgagcgtgg ggagggccgc gctgtcgctc ctccataaca tggacaagaa accgccgctc
     2941 attatcgtgg acaaacagat gccgggcatg gacggcatag agttcacgga gcacgtcagg
     3001 aactcgatcg gtagcggcat aaagatactg ctcctcacat catggggagg gatgagcttc
     3061 accgatatta aggacagggg cgttgacgag acgatagtta agcccgtgaa gagcacgaag
     3121 ctctacgaag cggtatccag gctcgtgagg gaagattcgt atctcggcgt cggtgagcag
     3181 gaccggcacg ggagctctcc gttcgaatcg gccagagcaa ggaaaagggc aacaccacgc
     3241 atactcgtcg tggacgacac ccctgataac cagaacctcg ctaagaggat cctcgaaatt
     3301 ggaggatacg tggtcgatgt cgcctcgggc ggcaaggaag gagtggatgc cgcgtgcgga
     3361 aatatctatg acctcatact tatggacata caaatgcctg tcgtggacgg cttcgacgcg
     3421 accaaggcga taaggagctg ggagaaggag aaccgcgcgg acaggacccc gatcgtcgcc
     3481 gttaccgcgc acgcactcaa gtggtacagg gagaagtgca tcgagaacga catggatgac
     3541 tacataacga agccgctcac gaagaagtcc ctcctcgaca tggcgaggaa atgggtgagc
     3601 gcggagagcg ctgaaagcgg ctcgccgggc gtcgacgttg gggcctgacc ttcccgggtt
     3661 tcgcgttccc tccgttccct tcggggaatc cccttggggt cgcgtttttt tccgtataga
     3721 attatgccgg acttacttcc cttaacagga gtgtgaccga atttgagtag gaatcagcct
     3781 gaactcgatc cggatatcgt cggggaaatc agagcgcggg gcatagagcc cgggctcgta
     3841 ttgtcacaga tagagatgtt cgagcggggc atgccccctg cccacctcgt cgggccctgc
     3901 accccggggg acggaatagt gacgatatgg ggcgaggccg agtcctacgc aaggctctat
     3961 gaatccggat cggcgcggag ggagatagtt aagttcgtgc cggct
//
LOCUS       DASSJH010000432         3831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_536957, whole genome shotgun sequence.
ACCESSION   DASSJH010000432 DASSJH010000000
VERSION     DASSJH010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536957"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VFJ67_09515"
     CDS             <1..944
                     /locus_tag="VFJ67_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET7290662.1"
                     /translation="HVLGFPPKGTHAHSMVQVFMALGEGELGAFRAYAEVYPDDCLLL
                     VDTINTLESGVPNAIKVFEELRSKGHKPVGIRLDSGDLAYLAIQSAKMLDDAGFPDTK
                     IVLSNQLDELVVWQVITQIEEEAPRYGLDPDALIKRLVYGVGTKLITSKGDSALDGVY
                     KLVAVRERREWTPAIKLSETPQKTLNPGNKMAWRIYDGRGYATADLLTIDEGDPGDMH
                     VLRMHHPSDHTKYRIIKRSEISRTEPLHVKVLDEGKLVYDFPPIDEIRKRRIADIESL
                     DTGVRRIMNPHIYHVSLSEKLWTLKQTLIRSIEKENA"
     gene            complement(990..2003)
                     /locus_tag="VFJ67_09520"
     CDS             complement(990..2003)
                     /locus_tag="VFJ67_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7290663.1"
                     /translation="MDNESRTFTAAAVQAAPVFLNKVETIGKIASLIEEAAANGASLI
                     VFPEAMIPCYPYWPKDIGTIEGRKLVLDAYIELHKNSIDIPGSDTDKLCEAAKKAGAY
                     VVIGVNERDGGTLYNTILYIDKQGSILGKHRKLMSIDSEKCIWGNGGAEDLAVFDTEV
                     GKVGGFFCYEHHMTLAKAAMFLKGEEIHAGLWAGHGFVKPTMDFASREYAFEGQVFVI
                     ASSGHITEDMVPDSFPLKKFTMWDYPGGSGIISPRGDYLAGPVYGKEDIIYADIDRDM
                     IIRSKAVIDGVGHFSRPDILTLEIRGYDPAKKKASNGSRGLKQKKSAKQPGAGKGSAS
                     KSN"
     gene            complement(2134..3012)
                     /locus_tag="VFJ67_09525"
     CDS             complement(2134..3012)
                     /locus_tag="VFJ67_09525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7290664.1"
                     /translation="MKFGIAVPNFGAFATWEHINELAGVAEELLYDSLWVSDHVIIPA
                     SHKVFGDTFLDPLVTLAHIAGSTNKIELGTSVIILPYRNPLVLAKMVSTLDTLSRGRV
                     ILGIGAGWLEDEFKALGVPFGERGKITDEYIEVIRELWTSDKPEYSGEYARFSDIRFL
                     PKPERKPHPPVWVGGESRKAIERAVRYGDGWHPFGLTPGVFREKAEYLRTLLPTEKKE
                     GFVMSLRRNIEINDKREVSPDETLRGGMEKITSGIREYGQAGVDHLILYILGRDFKAI
                     LRTLRVFAGEIRPALG"
     gene            complement(3055..>3831)
                     /locus_tag="VFJ67_09530"
     CDS             complement(3055..>3831)
                     /locus_tag="VFJ67_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET7290665.1"
                     /translation="SFVCTKEINMLRTAYDLFMISLLGFVKITLFFLFFCSSILFFAK
                     SNDLALDEKPAFAGLLASPGSGFLKTGSPTGKDDETDAAAPMLEEMTAEEAEIYFFIL
                     RLSESINQPNARKLAKLIVKECSNYDNLDPYLVLAVIQVESEFSPKALSKRGAIGLMQ
                     VMPGTAEYIAKEMGVDYNGKKTLYDPFVNVKLGIHYLSLLTDRYDSTENALDAYNYGP
                     SNFEKAQASDTDRKPSRYAKKVMNFKNYLEEESLLLAKNS"
BASE COUNT          807 a         1113 c          975 g          936 t
ORIGIN      
        1 ctcatgtgct cggttttccg ccgaaaggca cgcacgccca cagcatggtg caagtgttca
       61 tggcgttagg cgagggcgag ctgggcgctt tcagggccta cgccgaggtc tatccggatg
      121 actgccttct gctcgtcgat accataaaca cgctagagag cggcgtgccg aacgctataa
      181 aggtattcga ggagctgagg agcaaagggc ataaacccgt cggcataagg ctcgattcgg
      241 gcgacctcgc ctacctcgcg atacaatcgg cgaagatgct cgacgacgcc ggttttcccg
      301 atacaaaaat agtgctctcg aatcagctcg acgagctcgt cgtgtggcag gtgataacgc
      361 agatcgagga ggaagccccc cgctacgggc tcgaccccga cgctctcata aagaggctcg
      421 tctacggcgt cgggacgaag ctcataacgt cgaaaggcga ttccgcgctc gacggggttt
      481 acaagctcgt cgccgtgcgc gagaggcgtg agtggacgcc cgcgataaaa ttgtccgaga
      541 caccgcagaa aacactcaac cccggcaaca aaatggcctg gcgcatttat gacgggaggg
      601 gttacgcgac cgcggacctg ctcacgatag acgaaggcga tcccggagat atgcatgtgt
      661 taaggatgca tcacccttcc gaccatacga aatacaggat tataaaacgg agcgagataa
      721 gcaggacgga gcccctacac gtgaaggtat tggatgaggg gaagctagtt tacgatttcc
      781 ctcccataga cgagataagg aagaggagga tcgctgatat agagagcctc gacaccgggg
      841 tgcgtaggat aatgaatccc cacatatatc acgtctccct ttctgaaaaa ctctggacgc
      901 tcaagcagac gctcatccgc tcgatagaga aggaaaacgc ctgaccatat cccgtttcag
      961 cgctgcgcac cgtcccctgc ggcggggctt caattactct tggacgcgct tcccttcccc
     1021 gcgcccggct gtttcgcgga tttcttttgt tttaggcccc ggctcccgtt ggacgccttc
     1081 tttttagcgg gatcgtatcc ccttatctcg agtgtcagta tgtccggtct cgaaaaatgc
     1141 ccgaccccgt cgataacggc tttcgagcgt atgatcatgt ccctgtcaat gtccgcgtag
     1201 attatatctt ccttgccgta aactggtccc gccaggtagt ctcccctcgg gcttataatt
     1261 ccgctcccgc cggggtagtc ccacatggtg aattttttaa gagggaagct gtcggggacc
     1321 atgtcctcag ttatgtgccc tgaggacgcg atgacgaaca cctgtccttc gaacgcgtac
     1381 tcgcggctcg cgaaatccat cgtcggcttt acgaacccgt gtcccgccca gagccccgcg
     1441 tgtatctcct cccccttcag gaacatcgcg gcttttgcga gcgtcatgtg gtgctcgtaa
     1501 cagaagaacc cgcctacttt tccaacctcc gtatcgaaca cggcgaggtc ctcggcgccg
     1561 ccatttcccc atatgcactt ctcgctgtct atggacatga gcttcctgtg cttcccgagt
     1621 atgctgccct gtttgtctat gtagagtatt gtgttgtaga gagtgccgcc gtccctttcg
     1681 tttaccccta tgaccacgta cgcacccgct ttcttggccg cctcgcagag cttgtcggtg
     1741 tcgctccccg gtatatctat cgagttctta tgaagctcta tataggcgtc taaaacgagc
     1801 ttcctccctt ctatggtccc tatgtccttc ggccagtagg ggtagcaggg tatcatcgcc
     1861 tccggaaaca ctatgaggct cgcgccgttc gcggccgcct cttcgatgag gctcgctatc
     1921 tttcctatag tctccacctt gttgaggaat acgggcgccg cctgcaccgc ggccgccgtg
     1981 aatgtcctgc tctcgttatc catttgcggt tccccctgtc attcttgatt cggacccgaa
     2041 gccattatcc cacgaacccg aactccatgt caacgtaata agatctcttt accgatgtgt
     2101 cgctctatac ataaaagaat aatgccggaa taattatccg agcgccggcc ttatctcccc
     2161 ggcgaaaacc ctgagcgtcc tgagtatggc cttaaaatcc cttcccaata tatatagtat
     2221 aaggtgatcg acccccgcct gcccgtattc ccttatcccg ctcgttattt tttccatacc
     2281 tccccggagc gtctcgtccg ggctgacttc cctcttgtcg tttatttcga tgttccttct
     2341 gagagacatg acgaaccctt ctttcttttc ggtcgggagg agggttctca ggtactccgc
     2401 cttttcccgg aacaccccgg gcgtgagccc gaacgggtgc cagccgtccc cgtacctgac
     2461 cgcgcgctct atcgccttcc ggctctctcc cccgacccat acaggcgggt gcggtttcct
     2521 ctccggtttc ggcaggaatc taatgtccga gaaccgcgcg tattccccgc tatactccgg
     2581 cttatcgctc gtccaaagct ctcttatgac ctctatgtac tcgtccgtga tctttccccg
     2641 ctcaccgaac ggcacgccta gcgccttgaa ttcgtcctcg agccacccgg cgcctatgcc
     2701 caggatgacc ctcccccggg aaagcgtatc gagcgtcgag accatcttcg cgagaacaag
     2761 aggatttctg tacggcagga ttatcacgct cgtaccgagc tcgattttat tcgtggaacc
     2821 agctatgtgg gcgagcgtga cgagcgggtc gaggaatgtg tcgccgaaca ccttgtggga
     2881 tgcggggatg attacatggt cgcttaccca gagggagtcg tagaggagct cttcggcaac
     2941 cccggctaac tcgttaatat gctcccacgt cgcgaatgcc ccgaagttag gtaccgctat
     3001 tccgaatttc attcggaata aatattaaca tctccgggcg gaatgataat aagattagct
     3061 gttcttcgcc agaagcaggc tttcttcttc gagatagttt ttgaaattca taaccttctt
     3121 cgcgtatctc gaaggtttcc tgtccgtatc ggaagcctgg gccttttcga aattagacgg
     3181 gccgtaatta taagcgtcga gagcgttctc cgtggaatcg tacctgtcgg tcagcagaga
     3241 caggtaatga atgccgagct tcacgtttac gaacgggtca tagagtgttt ttttgccgtt
     3301 ataatcgacg cccatctcct ttgcgatata ttcggcggtc cccggcatca cctgcatcag
     3361 tccgatcgcg cctctcttgg ataatgcctt cggtgagaat tcactctcca cctgtatcac
     3421 cgccagtaca aggtacgggt ccagattgtc gtaattgctg cattccttta cgataagctt
     3481 ggcgagcttc cgggcgttcg gctggtttat cgattccgac agacggagaa taaagaaata
     3541 gatctcggct tcctcggctg tcatctcttc gagcatgggc gcggccgcat ccgtctcatc
     3601 atctttgccc gtcggagagc cggtctttaa gaacccgctc cccgggcttg cgagcaaacc
     3661 cgcgaatgcg ggtttttcat ccagggccag atcgtttgat ttagcgaaaa ataatattga
     3721 gctgcagaag aaaagaaaaa acaacgtgat tttgacgaat ccgagaagtg aaatcataaa
     3781 taaatcgtag gcggttcgca gcatatttat ctccttggtg cagacgaatg a
//
LOCUS       DASSJH010000433         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1402007, whole genome shotgun sequence.
ACCESSION   DASSJH010000433 DASSJH010000000
VERSION     DASSJH010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402007"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VFJ67_09535"
     CDS             complement(<1..1289)
                     /locus_tag="VFJ67_09535"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7290666.1"
                     /translation="MRKVQELKASQVYKVCDLGQLNIQTTEDLPPCADFIGQKRAVES
                     IEFGIGMDYAEYNIFLVGPTGVGKSTTIETILARVAKSKPTPGDWCYVYNFQDPNEPK
                     AIALPTGKGKVFHKDMEDFLQILKTDIPRAFESKEFEEQKQNLMNESQRKKNTLFEEL
                     QKTAAQNDIQIQFSPTGIITIPLLEGKPVSQEQYGALGEEVKKEIMNKKETVDNEVAE
                     ALKAARKIENEAVERLRELERRVALFAVRGLIESLSEKYHSFPKIIDYLDQVQKHILE
                     NIDNFLPDKNVQAAAGMPFPFRMPAQQPPTFTEYKVNVFIDNSLSEGAPVIFETHPTY
                     SNLFGSIEKEARFGALFTDFTMIRPGSLAKANGGYVVLEVLDMFKFPFVWDSLKKAIE
                     NQELRIEDIYQQFGYSSTIGLRPEPIKLDVKVIIAGNS"
     gene            1663..2661
                     /locus_tag="VFJ67_09540"
     CDS             1663..2661
                     /locus_tag="VFJ67_09540"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HET7290667.1"
                     /translation="MKHLASVILLILAFIALSCASHGPGGGGESGPGALFPVEKDFKW
                     GFIDGKGNVVIPYKYEGAVDFSEDRALVKVDGKWGYINRFDIMVISPQFEEAQKFSEG
                     LAQVKVDGRYGYIDRVGKIVSEPMFEKSEPFSEGLASVKINGRWGYINKEGKMVIEPR
                     YERAFSFSDGYALIVEDDKGGFIKKNGNIFIKPKYEWAEGYSEGLACVLADGKYVYID
                     EFGEVKLETGFEGARHFKEGAAAIMKNGKIGFINKSGLIIVEPEYDEAGDFSEGLATV
                     RRDGKWGFVNKRGEVVVEPRFFTAVSFSDGLAIVELGNGKWAYIDTKGRYVWKSFE"
BASE COUNT          590 a          692 c          786 g          701 t
ORIGIN      
        1 gagtttcctg cgattatcac ctttacatcg agcttaatag gctcgggcct gagccctatc
       61 gtgctcgagt acccgaactg ctggtatatg tcttcgatcc tcagctcctg gttctctatg
      121 gctttcttga gcgagtccca cacgaaaggg aatttgaaca tatcgagcac ttcgagcact
      181 acgtatccgc cgttcgcctt ggcaagcgac cccgggcgga tcattgtgaa atcggtgaac
      241 agcgcgccga acctcgcctc tttttctatt gagccgaaga ggttcgaata agtggggtgt
      301 gtttcgaaaa tcacgggcgc gccttccgag agggaattgt ctatgaacac gtttaccttg
      361 tattcggtga acgtcggagg ctgctgcgcg ggcattctga aaggaaacgg catcccggct
      421 gcagcctgaa cgttcttatc cggcaggaag ttgtctatgt tctcaagtat gtgtttctgc
      481 acctggtcca ggtaatcgat tatcttgggg aagctgtgat atttctcgct caggctttct
      541 ataaggcccc tgaccgcgaa cagggctacg cgcctttcga gctccctcag cctctcgacg
      601 gcctcgtttt cgatcttccg ggccgctttc agggcctccg cgacctcgtt gtcgacggtt
      661 tcctttttat tcataatctc tttcttgacc tcttcaccga gcgcgccgta ctgctcctga
      721 gatacgggct tcccttcgag aagcggaatc gttattatcc cggtcggtga gaactggatc
      781 tgtatgtcgt tctgggcggc cgttttctgg agctcctcga agagcgtgtt cttcttcctc
      841 tgggactcgt tcatcagatt ttgcttctgc tcttcgaact ccttgctctc aaacgccctg
      901 gggatgtccg ttttcagtat ctggagaaag tcctccatgt ccttgtgaaa gacctttccc
      961 ttccctgtcg ggagcgcaat ggccttgggc tcgttcgggt cctgaaagtt gtacacgtag
     1021 caccagtccc ccggcgtggg cttcgatttg gcgaccctcg cgagtatggt ctctatggta
     1081 gtcgacttcc ccacccccgt gggcccgacg agaaatatat tgtattcggc gtaatccatc
     1141 ccgatgccga actctatcga ttcgacggcc ctcttctgcc ctatgaaatc cgcgcacgga
     1201 ggcaggtctt ccgtggtttg aatgttgagc tggcccagat cgcaaacctt gtaaacctgc
     1261 gaggccttga gttcctgtac ttttctcatt aattttcccc tttgtgtaag tccggcgcgc
     1321 gaaatctaat ccgtaaccgt gagaaatatt accatctcgc tatatgcaat caaagcttca
     1381 caaattccat atagcggctg cgggtatatc cagattattc tgtatttacg gcgaataggc
     1441 aagttatttc ggatgcgcca gcgcggcccc gctaacgata ccggaaatgc gatttgtact
     1501 gaaaagttga atggtttagt caatgccgga aggcatcggt tgaaaaaccg ctccgggcgg
     1561 ttccttttaa aaattctcat tttgtttatc attaatgccg gcttataagc gaatgacacg
     1621 gtctgaattg agcgtccggt tacgggaacg gggagacaag gtttgaagca tcttgcatcg
     1681 gtgatacttt tgatactggc gtttattgcg ctctcctgcg cgtcgcacgg ccccgggggc
     1741 ggcggagagt ccgggcccgg agcgctcttc cccgtggaaa aggacttcaa gtggggtttc
     1801 atagacggaa aaggcaacgt cgtaatacct tacaaatacg agggcgccgt cgatttttcc
     1861 gaggacaggg cgctcgtcaa agtggacggt aagtggggct atataaaccg gttcgacatc
     1921 atggtcatca gcccgcagtt cgaggaagcg cagaaatttt cggaagggct agcgcaggtg
     1981 aaggtcgacg gacggtacgg gtatatagac agggtcggca agatcgtttc cgagccaatg
     2041 ttcgagaagt ccgagccgtt ttccgaaggg cttgcgagcg tgaagataaa cggcaggtgg
     2101 ggttacatta ataaagaagg caaaatggtg atagagcccc gttacgagag ggctttcagc
     2161 ttctcggacg gctatgcgct gatagtggag gacgacaagg gcgggtttat aaagaagaac
     2221 gggaacattt tcatcaaacc gaaatacgaa tgggcggagg gatactcgga agggctagca
     2281 tgcgtgcttg cggacggcaa atacgtttac atcgacgagt tcggagaggt gaagctcgag
     2341 accggcttcg aaggggccag gcatttcaag gaaggggcgg ccgcgataat gaagaacggc
     2401 aagatcggct ttattaacaa gagcggcctc ataatagtag agcccgaata cgacgaggcg
     2461 ggggattttt cggaaggtct cgccacggtg aggcgggacg gcaagtgggg ttttgtcaat
     2521 aagaggggag aggtcgtggt ggagccccgg ttttttacgg ctgtcagttt ctcggacggg
     2581 ctcgccatag tggagctcgg aaacggcaaa tgggcttaca tagacacgaa gggccgttac
     2641 gtatggaaat ccttcgaatg agatactcgg gtattaccgg ctgttccctt accccgcgcc
     2701 aatctcctgg ggttttttat ccggctgctc caccgccgct tgggcggcgt ctccgttccg
     2761 cgcgggctt
//
LOCUS       DASSJH010000434         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_320935, whole genome shotgun sequence.
ACCESSION   DASSJH010000434 DASSJH010000000
VERSION     DASSJH010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320935"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..898)
                     /gene="murQ"
                     /locus_tag="VFJ67_09545"
     CDS             complement(2..898)
                     /gene="murQ"
                     /locus_tag="VFJ67_09545"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HET7290668.1"
                     /translation="MTKSRKTGHLLTEKINPRTAHLDQCSTDEIVRLILAEDGMIPGA
                     VQKEKRRIAEAVELIVERMVRGGRLIFIGAGTSGRLGVMEAAECPPTFGTDPSLIRAI
                     IAGGRKAFWRSVEGAEDSAKEARKELRKINLGENDVLVGIAASATTPFVESALSYAQK
                     KGSGKILVTCNRADTGIPDVVVSILAGPEAIVGSTRMKAGTATKMVLNMLTTATMVKL
                     GKTYGNLMVDVLPRSAKLRDRAVRIVTEIAVVDEREAGRLLRKSGWDVKASVVMAKKG
                     IGYKKAKDLLFKNNGFLRKALS"
     gene            994..1069
                     /locus_tag="VFJ67_09550"
     tRNA            994..1069
                     /locus_tag="VFJ67_09550"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1027..1029,aa:Gly,seq:ccc)
     gene            complement(1116..>2246)
                     /locus_tag="VFJ67_09555"
     CDS             complement(1116..>2246)
                     /locus_tag="VFJ67_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HET7290669.1"
                     /translation="TLYSSANLDVSNEPYVLSLPDENGRYYLMPMLSGWTNVFTDPGS
                     RTTGTGAQTYAITGPGWKGTLPDGVKEIKSPTSIVWIIGRTYCTGTPEDYKAVHAIQD
                     EYKLVPLSAYGKPYTPPAGKVDPSIDMKTAVRDQVNALDAEAYFKLLAELMKTNPPAP
                     GDAPMIANMAHIGIIPGQDFDITKLAPEIKKGLEGAPKAAQEKIMAVAGKPGKLVNGW
                     AVLTDLGSYGTNYVVRAVVTAVGLGANLPQDAVYPKTQLDGSGNTLNGANSYVLTFPK
                     GQTPPVKGFWSLTMYDAQYFFVDNPLNRYTLSPRNDLKYNADGSLDLYIQNTSPGADK
                     ESNWLPAPKDDFILMLRLYWPNETPPSILDGTWKPPAVTKTE"
BASE COUNT          418 a          650 c          604 g          574 t
ORIGIN      
        1 atcagcttaa cgcttttcgt aagaagccgt tatttttgaa taataaatcc ttcgcttttt
       61 tatagcctat accctttttc gccatcacca cggacgcctt cacgtcccag ccggatttcc
      121 tgagcagcct tccagcctcc cgctcgtcca ctaccgcgat ctctgtaaca attcgcaccg
      181 ccctgtccct gagcttggcc gagcgtggca ggacgtcgac catgaggttg ccgtatgtct
      241 ttccgagctt gaccatggtg gccgtcgtga gcatgttcag aaccattttt gtcgcggtgc
      301 cggccttcat gcgtgtagag ccgacgatcg cttcagggcc cgctaggatt gagacgacga
      361 cgtccggaat tcctgtatcg gctcggttgc acgtgacgag tatctttccg ctccccttct
      421 tttgagcgta cgagagggcg ctctcgacga acggagtggt cgcgctcgcc gctattccaa
      481 cgaggacgtc attttccccg agattgatct tcctgagctc cttccgggcc tccttcgccg
      541 agtcttcggc cccttcgacc gacctccaga acgctttcct gcccccggct attatcgccc
      601 ttatgagcga ggggtcggtc ccgaaagttg gcgggcattc ggcagcttcc ataactccga
      661 gcctcccgct cgtccctgcg cctatgaata tcaggcgtcc gccccggacc atgcgctcga
      721 cgattaattc cacggcctcc gctatgcgcc gcttctcctt ctgaaccgct cccgggatca
      781 tgccgtcctc tgcgaggatc agcctgacta tctcgtccgt cgaacactgg tcgaggtgcg
      841 cggtgcgggg gtttattttt tcggtgagga ggtgtcctgt cttccggctc tttgtcacgg
      901 ggataattat atcccgaaga ggccgaatta ttaacacggg ggatattatt taattgacaa
      961 taccgcggcc atgtaataga ttttcaggtg aaagcgggtg tagctcagct ggtagagcat
     1021 aagcttccca agcttagggt cgcgggttcg aatcccgtcg cccgctccat ttttcccccg
     1081 taacgcagtc agtcaacaag ccaaataaac gagagctatt ccgtcttggt cactgccggt
     1141 ggtttccagg ttccgtccag gatcgaaggg ggcgtctcgt tcggccagta gagcctcagc
     1201 atgagtatga aatcgtcttt cggagcgggg agccaattgg attccttgtc cgcgcccggg
     1261 gatgtatttt gtatatacag atcgagagag ccgtcggcgt tgtatttgag atcgttcctg
     1321 ggactcaatg tataacggtt gagcggatta tcaacgaaga aatattgtgc gtcatacatt
     1381 gtcagagacc agaacccctt cacgggcggg gtctgtcctt taggaaacgt gaggacataa
     1441 ctattcgctc cattgagggt attgccgctg ccgtccagct gagtcttggg ataaacagcg
     1501 tcctgaggta ggtttgcgcc aaggccgaca gccgtgacga ccgcacggac tacgtaattc
     1561 gtgccgtaac ttccgaggtc cgttaagacc gcccatccgt tcacgagctt gcccggtttc
     1621 ccggcaactg ccattatctt ttcctgggcc gccttgggag cgccttcgag accttttttg
     1681 atctcaggtg cgagcttcgt tatatcgaag tcttgtcccg gaattatgcc tatgtgagcc
     1741 atattcgcaa tcatcggagc gtccccgggc gcgggtgggt tcgttttcat cagctccgcc
     1801 aacagcttga agtaagcctc cgcatcgagc gcgttcacct ggtccctcac ggccgttttc
     1861 atatctattg acggatcgac cttcccggcg ggcggagtat atggtttccc gtaagcgctc
     1921 agcgggacga gcttgtattc gtcctggatc gcatgcacag ccttatagtc ctcaggcgtt
     1981 ccggtgcagt acgttctccc tattatccac acgatgctcg tcggcgactt gatctctttc
     2041 acgccgtcag gcagtgtgcc cttccatccg ggcccggtta tggcgtatgt ctgggcgcct
     2101 gtgcccgtag tgcgtgaccc ggggtcggtg aatacgttcg tccagccgga gagcatgggc
     2161 atcagatagt agcggccgtt ctcatccggc agactcaaaa cgtaaggctc attcgaaacg
     2221 tcaagattcg cggatgaata aagcgt
//
LOCUS       DASSJH010000435         6804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_213005, whole genome shotgun sequence.
ACCESSION   DASSJH010000435 DASSJH010000000
VERSION     DASSJH010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213005"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /gene="sbcD"
                     /locus_tag="VFJ67_09560"
     CDS             complement(<1..514)
                     /gene="sbcD"
                     /locus_tag="VFJ67_09560"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HET7290670.1"
                     /translation="MRKIRFIHLSDIHIGLLTHGRLNPSTGYNTRLEDILKCLDLVLD
                     TAARESADLILIAGDVFHRENPHPAEEIEFARRIGRAAGKDGARVVIVLGNHDYSSSG
                     LSASAVEIFPALEIEGVTIARKPGLIKVDTRSGMVQVACLPWAGRGMLLSRDEYRSLS
                     PQELQAEIEGK"
     gene            complement(566..1705)
                     /locus_tag="VFJ67_09565"
     CDS             complement(566..1705)
                     /locus_tag="VFJ67_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET7290671.1"
                     /translation="MKNYIFTPGPVAVPSEILIEMAKPIMHHRTKEFEAIFAEVREGL
                     KYIYDTKQEVFVLAASGTGAMEAAVVNTLSRGDKVLVVNGGKFGERWGKICKAYGLDT
                     QELMVEWGHSVNPADIEKALEKDPKIRAVLVQASETSTGVMHPTKEIADLTRHRDDVI
                     LIVDGITGVGVFPLPFDELGIDVMVGGSQKAFMLPPGLSFAAMSDKAWEFNKTSNLPK
                     YYFDFKAALKNAEKNTTPYTPAVTLIIGLAAVLRRFREEGRESMYKRHAKLSLAMREG
                     MKAIGLDIYVKDVPSPALTTVVAPPEIGSSKVIKALAGEFGITVADGQDQAKGVIFRV
                     SHIGDLDKTDTLFFISAIESVLHKLGYRFNYGAGTKKASEILFGE"
     gene            1961..3071
                     /gene="prfB"
                     /locus_tag="VFJ67_09570"
     CDS             join(1961..2029,2031..3071)
                     /gene="prfB"
                     /locus_tag="VFJ67_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139172183.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET7290672.1"
                     /translation="MFEENKEQIQELKKRLVVLGEFLDLPGKNSRLKELEEITGKEGF
                     WDNAEKAQEILREQSRIKSTLDSLKRLSSDLEDVEVLEELSAEEEDEDAAREAREKLS
                     EITSRVETLEFKRILGDPDDERNAIVSINAGAGGTEAQDWAEMLLRMYLRYAELNGFT
                     TEFIEYQEGDEAGMKSATFLVKGDYAFGYLKGESGVHRLVRISPFDSNKRRHTSFASV
                     FVSPEIDESIEVEIEEKDLRVDTYRASGAGGQHVNKTDSAIRITHFPTGIVVSCQNER
                     SQHQNRATAMKILKARLYELEKEKQREKIDELNASKKEIGWGSQIRSYVLHPYRMIKD
                     HRTGFETGNVEPVLDGDLSEFIKAYLQMRAAEKPL"
     gene            3204..4076
                     /locus_tag="VFJ67_09575"
     CDS             3204..4076
                     /locus_tag="VFJ67_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET7290673.1"
                     /translation="MLRVGIIGKTNINKTYEIAKELCAWFKQRGVEVVLEKEIGSKIR
                     RKNSAPAAEIPELVDIILVFGGDGTFLGVARLACGHGTPLLGINLGGLGFLTEITVDE
                     IYPMMERILSGDYEIEDRCMLRTTVRRGKTGAKHYEVLNDVVINKEAEARIIDLEVFI
                     EGSHVTTYKADGIILSTPTGSTAYSLSAGGPIVHPTLPVTIITPICPHTLTNRPLVVS
                     NEMKVEIKITTQEPDTYLTLDGQIGMRLRTGDVIEVRRSDTLVKLIKSPFRDYFTILR
                     TKLMWGERYGKING"
     gene            4057..4425
                     /locus_tag="VFJ67_09580"
     CDS             4057..4425
                     /locus_tag="VFJ67_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290674.1"
                     /translation="MARSTDEQFLHDWVIRKVHEKYSRLYSEVRINPGEETNFDYKGK
                     YPDIVIVNYGQVVQIIEVETRETINTDRVAKWKEMSELGVKFTLMVPKELQAAARDIC
                     WKNGLAAKVNLGSFDVQLAT"
     gene            complement(4435..6480)
                     /locus_tag="VFJ67_09585"
     CDS             complement(4435..6480)
                     /locus_tag="VFJ67_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7290675.1"
                     /translation="MKSKFKQEGSSDIGLKPSPIGFTESGEPFGGDESSFIPYDESEA
                     ESNSGFFFELLENAPDSFFGLTPDGVITYINPAFEALTGWERGEWIGRPLSDLFVENW
                     MGDAVRDSLKLEYDGQRRVIEADIRLNGGGSLNAEILVSVYTREGKYASIVGSIRDIT
                     GRKRSDEAHTLQEKRIRSLYEISAASGMGIDTQLVETLRTGACMLGMEVAGVGKLLGK
                     SCKVLYSYDETGRVVQGLECDLSKMFCKITLANDDVVALDHVGESEYSNEEFYKGFGF
                     ESYIGVPLRIDGKVFGTLNFASPYPRKEPFTTADKDFIRLMGRWIGTMIERKKAEDML
                     KEKEELYRTLVENAHDLIIEILYDGRIMYASSNHKEILGYEPGELLGKNVFELMHPED
                     RKAIMSAFMQGFGRGAAGKGVYRYMHRDGEWRWFEATGKPFRTSTGEIRAIVDTREIT
                     ERKLAEEKIQESLQQKESLLREIHHRVKNNLQVISSLLSLQSGYAKDEESLAHFYESQ
                     SRISTIALIHEKLYHSRNIDEIKMLDYITDLTRNLLRVYKDLGGNIDVQINAYDISID
                     IDRAIPCGLIINELFSNCLKHAFRGAERGETSRKRRNKITVEINSDPEGLLVLSVKDN
                     GVGFPGNVDIRNTDTLGLQLVTTLADQLKGKIEIANDNGAAFTIVFPLLAKKPGGPE"
BASE COUNT         1595 a         1844 c         1818 g         1547 t
ORIGIN      
        1 atttcccttc gatctcggcc tggagctcct gcggggaaag cgacctgtac tcgtccctcg
       61 agagcagcat tccccttccc gcccagggga gacaggcgac ctggaccata ccgctcctcg
      121 tgtctacttt tatcagcccg ggttttcggg caatggtaac gccttcgatt tcgagggccg
      181 ggaatatctc cacggctgac gcgctcaagc cggacgacga ataatcgtgg ttcccgagca
      241 ctatcacgac acgggccccg tcttttccgg ctgcccttcc tatcctgcgt gcgaactcga
      301 tctcttcggc gggatggggg ttctccctgt ggaacacgtc gcccgcgatg agtatgaggt
      361 cagcggattc gcgggctgct gtgtcgagca cgaggtcgag acacttgaga atgtcctcga
      421 gtctcgtgtt ataccccgta gaagggttca gcctgccgtg agtcagaagt ccgatatgta
      481 tatcggaaag gtggatgaac cttatctttc tcaaccgttt ccgggcgtcc cggattggtg
      541 aatatgctaa gggacgcttc ctctcttatt ctccgaacag gatctcggat gcctttttcg
      601 tgcccgctcc gtagttgaac ctgtacccga gcttgtggag cacgctttct atcgccgata
      661 tgaagaacag agtatccgtc ttgtccaggt cgcctatgtg ggacacgcgg aatataactc
      721 ccttggcctg gtcctgaccg tcggcgactg tgatgccgaa ttcgccggcg agagccttta
      781 tgaccttcga ggagcctatc tcgggcgggg cgacgacggt ggtgagcgcc gggctcggga
      841 cgtccttcac gtatatatcg agccctatcg ccttcatacc ctccctcatg gcgagagaaa
      901 gcttcgcgtg gcgcttgtac atgctctccc ttccctcttc cctgaacctc ctcagcaccg
      961 cggcgagccc tatgatgagc gtgaccgcag gggtataggg ggtagtgttc ttctccgcgt
     1021 tcttaagcgc ggctttgaaa tcgaagtaat atttcggcag gttggaggtc ttgttgaact
     1081 cccacgcctt gtcgctcatg gcggcgaaag acagacccgg cggcagcatg aacgccttct
     1141 gggaacctcc caccatcacg tctatgccga gctcgtcgaa ggggagcggg aacacgccta
     1201 cgcctgttat gccgtcgact atgagtatga cgtcgtcacg gtgtctcgtg aggtcggcga
     1261 tttctttcgt cgggtgcatg acgcctgtcg aggtctcgga cgcctgcacg agcacagcgc
     1321 ggattttggg gtctttctcg agcgcttttt ctatatcggc cgggtttacg gaatgtcccc
     1381 attcgaccat cagctcctga gtgtcgagcc cgtaggcctt gcatatcttt ccccacctct
     1441 ccccgaattt accgccgttc acgacgagca ccttgtcacc gcgcgagagg gtattcacaa
     1501 cagccgcctc catagcgccc gtacccgaag cagccaggac gaacacctcc tgcttcgtat
     1561 cgtatatgta cttgagccct tcccttactt cggcaaatat ggcttcgaac tctttcgtcc
     1621 tgtggtgcat gatgggcttt gccatctcta tgagaatttc gcttgggaca gccaccggcc
     1681 ccggggtaaa tatgtagttt ttcactttcg gattctcctt tgtaatgacg ttctttcaga
     1741 tttttgaaaa accggacagc ccgaccaaga tgcccggctg aaaagcccgc cggccggcca
     1801 tgccggcatc gagccagcta actcatccca aggtaaattc ccgtgatgca agccgctata
     1861 aaaccataag aatctaccca aagctacgga taattcaact gtgcttgctt ttgagtcccc
     1921 gagccatata ttatttacat aaacaaaaag ggagaagaaa atgttcgaag aaaataaaga
     1981 gcaaatccaa gaacttaaga agagactcgt cgtcctaggg gagtttcttt gacctgccgg
     2041 gaaagaattc ccgcttaaaa gagctcgaag agataacggg gaaagagggc ttctgggaca
     2101 acgccgaaaa agcccaggaa atactcaggg aacagtcccg cataaagagc acgctcgaca
     2161 gcctgaagag gttatccagc gacctcgagg acgtcgaggt actggaggag ctttccgccg
     2221 aagaggaaga cgaggatgcg gcgagggagg ccagggaaaa actatcggag ataacgagcc
     2281 gcgtcgagac gctcgagttc aagcgaatac tcggcgaccc ggacgacgag agaaacgcaa
     2341 tcgtatcgat caatgccggg gccgggggaa ccgaggcgca ggactgggcc gagatgctcc
     2401 tcaggatgta tctgagatac gcagagctga acggttttac gacagagttc attgaatatc
     2461 aggaggggga cgaggcggga atgaagagcg ccacgttcct cgtaaagggg gactacgctt
     2521 tcggttacct gaagggcgaa agcggcgtac acagactggt cagaatatct cccttcgact
     2581 ccaataaaag gaggcacacg tcgtttgcct ccgtgttcgt atcccccgag atagacgaga
     2641 gcatagaggt cgagatagag gaaaaagact tgagggtcga tacctaccgc gcaagcggag
     2701 cggggggtca gcacgtcaac aaaacggact ccgcgatcag gatcacccat ttcccgaccg
     2761 ggatagtagt gagctgccag aacgagcgct cccagcacca gaaccgggcg acggccatga
     2821 agatactgaa ggccagactc tacgagctcg aaaaggaaaa gcagagagaa aagatagacg
     2881 agctcaacgc gtcgaaaaag gagatcgggt gggggagcca gatacgctcc tacgtgcttc
     2941 atccgtacag gatgataaag gaccacagga ccggctttga gaccggaaac gtcgagcccg
     3001 ttctcgacgg cgacctgagc gaatttataa aggcctatct ccagatgcgg gcggccgaga
     3061 aaccgctcta aagcccggat cgagaccgac cgattaccga aagcacagtc caatcctatg
     3121 gtttccggat accgggtgtt atatttatcc atctataccg cccggaatcg agtggagccc
     3181 gtgtccgtca ggggaggaaa tgaattttga gagtcggaat catagggaaa acgaatataa
     3241 acaagacata cgagatcgcg aaagagctct gcgcatggtt taagcagagg ggggtcgagg
     3301 tcgttctcga gaaagagatc gggagcaaga tcaggagaaa gaattcagcc cccgcagcag
     3361 aaatccccga gctcgtcgac ataatactcg tattcggagg ggacgggacg ttcctgggcg
     3421 tggccaggct ggcgtgcggg cacggcactc cgctgctcgg cattaatctg ggcgggctcg
     3481 gttttctgac cgagattacg gtcgatgaga tatatccgat gatggagagg atactgagcg
     3541 gcgattacga gatcgaggac cgctgcatgc tcaggacgac tgtcaggagg gggaaaacag
     3601 gagcgaagca ctatgaggtg ctgaacgatg tggttattaa caaagaggca gaagcgcgta
     3661 ttatagacct cgaagttttt atcgagggct cgcacgtaac gacgtataaa gcggacggca
     3721 taatcctctc tactcccaca ggctcgaccg cttattccct ctcggcggga gggccgatag
     3781 tgcatcccac gctgcccgtc accataatca ccccgatctg tccccatacc cttacgaaca
     3841 gaccgctcgt ggtctcgaac gaaatgaagg tcgagataaa aatcacgacg caggagccgg
     3901 acacctatct cactctcgac ggccagatcg gcatgagact caggaccgga gacgttatag
     3961 aagtcaggag atccgatacc ctcgtcaagc tgatcaagtc ccctttcagg gattacttca
     4021 ccatcctcag gaccaagctt atgtggggtg agagatatgg caagatcaac ggatgagcag
     4081 ttccttcacg actgggttat caggaaggtg cacgagaaat actcgaggct ctatagcgag
     4141 gtccgcatca atcccggcga ggagacgaac ttcgattaca agggtaagta cccggacatc
     4201 gtgatcgtga actacgggca ggtcgtgcag ataatagagg ttgaaacgag agagactata
     4261 aacaccgaca gggttgccaa gtggaaggag atgtccgagc tcggcgtgaa atttaccctc
     4321 atggttccca aagagcttca ggctgccgcg agggatatat gctggaagaa cgggcttgca
     4381 gccaaggtca acctcggcag cttcgacgta cagttggcca cgtagccgcc ggtgttactc
     4441 aggaccgccg ggtttcttcg ccagaagcgg gaagacgata gtgaaggcag ccccgttatc
     4501 gttcgcgatc tctatttttc cctttaactg atccgcaagg gtcgtgacga gctggagtcc
     4561 cagcgtatcc gtgttcctga tgtccacatt tccggggaaa ccgacgccgt tatcctttac
     4621 ggagaggacc agtagtccct ccggatccga atttatctcc accgttattt tatttctccg
     4681 cttacgggat gtttcccctc tctccgcgcc ccggaaggcg tgtttgagac aattcgagaa
     4741 aagctcgttg atgatgagtc cgcatgggat ggcgcggtcg atgtctatcg atatatcgta
     4801 agcgtttatt tgaacgtcta tatttccccc gaggtcttta tataccctga gaaggtttct
     4861 ggtcaggtcc gtgatatagt cgagcatctt tatctcgtca atatttctcg aatggtagag
     4921 cttctcgtgt ataagagcaa tggtcgatat cctactctgg ctttcataga aatgggcaag
     4981 cgattcctcg tccttcgcgt agcccgactg aaggctcaga aggctcgata tgacctgaag
     5041 gttattttta accctatggt ggatttccct gaggagagac tctttctgct ggagcgattc
     5101 ctgaatcttt tcctcggcca atttccgctc tgtgatctca cgcgtatcga ctatagccct
     5161 tatctcgccc gtagacgtcc tgaacggttt cccggtggcc tcgaaccagc gccactctcc
     5221 gtctctatgc atgtacctgt aaacaccctt gccagccgcc ccccttccga agccctgcat
     5281 aaaggctgac atgattgcct tgcggtcttc ggggtgcatc aattcgaata cgtttttccc
     5341 cagaagctca cccggctcgt atccaagtat ttctttgtgg ttggagctcg catacatgat
     5401 gcgtccgtcg tacagtatct cgattatcag gtcgtgagcg ttttcgacga gcgtgcggta
     5461 taactcttct ttttccttga gcatgtcctc ggccttcttc cgctctatca tcgtgccgat
     5521 ccagcgcccc atcagacgga taaaatcctt atcagccgtc gtaaagggtt ccttccgggg
     5581 ataggggctc gcgaaattaa gggtcccgaa taccttgccg tcgatcctca agggcacgcc
     5641 tatgtatgac tcgaaaccga aacccttgta gaactcttcg ttgctatact cggactcgcc
     5701 tacatggtcg agcgcaacga cgtcatcgtt ggcaagcgta attttgcaga acatttttga
     5761 cagatcgcat tcgaggccct gtaccaccct tcccgtctcg tcgtagctgt agagaacctt
     5821 gcagctcttg cccaagagct tgccgactcc ggcaacctcc ataccgagca tacaggcccc
     5881 tgtcctgagt gtctcgacaa gctgagtatc gatccccatc cccgacgcag cggagatctc
     5941 ataaagggac ctgatccgtt tttcctggag cgtgtgagcc tcatcggagc gcttccttcc
     6001 cgtgatgtcc ctgatggagc ccacgatgct tgcatactta ccttcccgcg tataaaccga
     6061 aaccaagatc tcggcattca gggatccccc tccattcaaa cggatatcgg cttcgatgac
     6121 ccgcctttga ccgtcgtatt ctaacttgag cgaatctcgc acggcgtcac ccatccagtt
     6181 ttcgacgaag aggtccgaga gcggcctgcc tatccattcg ccgcgctccc agccggtcag
     6241 agcttcaaat gccggattaa tataggtaat aacaccatcc ggtgtgaggc cgaaaaaaga
     6301 atcgggcgca ttctccagta gctcgaagaa aaatccgctg ttgctttcag cctcgctttc
     6361 atcataagga atgaaagagc tttcatcccc accgaatggt tcaccggatt ccgtgaaacc
     6421 gattggggaa ggttttaatc ctatatccga tgaaccctct tgcttgaatt tgctcttcat
     6481 aaaaaagtcg ctcgcggcgg gcaaaagtcg gtgggggatg ctctgttccg accgtcccgg
     6541 gccgcagtct tgcatttttt cctggatcgc cggacgccgt ccttaattct aataaaattt
     6601 gcgaatacat gcaatccgat gcgcatactc tcccggagta attacatatt taagaagaat
     6661 ttgaaacgac acgcggataa cgccaggctt gccaattcct ctaaatttag ataggctttt
     6721 catgaactta gggggtatac gtccgatgtc caatctcgag acagtcgatc cggaaattta
     6781 ccgcgccata aagtcggaag tcga
//
LOCUS       DASSJH010000436         8237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1402490, whole genome shotgun sequence.
ACCESSION   DASSJH010000436 DASSJH010000000
VERSION     DASSJH010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402490"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFJ67_09590"
     CDS             complement(<1..612)
                     /locus_tag="VFJ67_09590"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HET7290676.1"
                     /translation="MKEKEEKPFMRTFPKPVVVTSRCFEFDACRYNGQMIPNNFVQAL
                     EPWVELRPVCPELEIGLGVPRDPVRIVSKGGERKLVQPSTGRDLTSLMREFSARYLDT
                     LGDADGFILKSRSPSCGLGDAKEFPDAVSEIAAGKGAGFFAEAVIGRFPGLAIEDEGR
                     LRNFRIREHFLTKLYTLALFREARKVGTANALVNFHTDNKLLLM"
     gene            complement(609..1394)
                     /locus_tag="VFJ67_09595"
     CDS             complement(609..1394)
                     /locus_tag="VFJ67_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290677.1"
                     /translation="MALSVLMTYEPLEKTGERYDYWRSYISFYERFKDGPAAPNTAWY
                     GSEYDAFRESLSKELGIEKRLIEDCFFMKDEDGKYYVTPVGQKANPNIFSAENFIPLE
                     WFLMYEGAEKKYFFTHTGFGAIRQDSIYYITRVGSALERLEAAVSDIDAFLASPESAG
                     SPALAGILSDLKAGGESLTHWLGGFDRESFVLLNYGEMSSRIDPDQIKNEDSVSELRL
                     VLTLAREGSAEDAEKVLAMLLSRWEGINRSFSDDAEKPTTTLQ"
     gene            complement(1459..2835)
                     /locus_tag="VFJ67_09600"
     CDS             complement(1459..2835)
                     /locus_tag="VFJ67_09600"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET7290678.1"
                     /translation="MIHGKAIFWFKRDLRTDDNTGLLNAVRDSREVIPLYVLEDSILS
                     KYSSGSKRLGFLADALLKLDSELRRLGSYLLVLRGKAEEIIPSLIGSQKVEAVYTNRA
                     YGFSGVKRDLGVEHQCRLHDVTFRKYDDTFLVPPHEIDQRKVFTPFYGIWQGKTKRPA
                     LDAPGKINSPAVTASPAENVLRDLGGAGNIYWPIDFAARRLRDFNFGDYGMTRDFPYL
                     DGTSRLSPYLRFGIVSVRRVYEAATRASSDPGSYISELAWREFWYHIMHNFPETRGLE
                     FQEKRRNIKWINSEPWYDAWREGRTGYPIVDAGMRQLREEGWIHNRVRMIVASFLTKD
                     LITDWRLGDRHFFEHLVDYDETVDIGNWQWSASCGADPKPFRIFNPILQSQNYDPECL
                     YIKKYIPDLSAVDPEKIHNPLTYKLPYPRPIVNHYEMRNLAHEAYSGGRIDDQYISQI
                     KKDTGLPT"
     gene            complement(3557..4675)
                     /locus_tag="VFJ67_09605"
     CDS             complement(3557..4675)
                     /locus_tag="VFJ67_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7290679.1"
                     /translation="MMDNEFDKNYFSEEGYQDDEFFVRGGKGENSGNSFADTEGSPAV
                     SPAGKRKGKERKWTKYEEERVLQVYYRDLLTEPLLTPDDEKQLGAKIKECEKKARMIQ
                     KDIERLTRARLGKKAGDKNRLDATRKVFSEKAQELKSKFIRSNLRLVVSIAKRHLGRG
                     LPLTDLVQEGNLGLMRAVDKFDHRKGFKFSTYAAWWIQQALTRAIAEKTRTIKVPVYV
                     LEQSGKVFRAKYALEEELGRKPYPEEIAEKAGLSREVVQAVLDGTDSLLSLDNPVADD
                     NERTYVDLMPDSREGQEAAVSDIRVKSLLEECLSSLTPKEEEIVRMRYGLGDEKVYTL
                     DEIGNKFGVTRERIRQIEKAALEKISKSGLGDTLKGYL"
     gene            complement(4789..5253)
                     /locus_tag="VFJ67_09610"
     CDS             complement(4789..5253)
                     /locus_tag="VFJ67_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET7290680.1"
                     /translation="MGLSTKVPFTDAEAVEELDIVETAAAAGSFNTLVAAVNAAGLTE
                     TLKGVGPFTVFAPTDEAFAKLPEGTLEMLLKPENKEKLAAILTYHVVSGKVTAEEVVK
                     LSSAESVSGKKIDIKTSDGKVMVDGATVIKTDVMAENGVIHVIDTVMMPPAD"
     gene            5679..6614
                     /locus_tag="VFJ67_09615"
     CDS             5679..6614
                     /locus_tag="VFJ67_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7290681.1"
                     /translation="MKVASRMSGLTPHAIRAWERRYGVVKPGRDSNNRRFYRESDIER
                     LTLLHKATDAGHPISQIAGYSKEELRGIVGSMNVIDLPSRKPAEMRSTGDLTLVRDYN
                     AYVEEIIAAVEKMDRKSLEETLISVETEIGRNALLERVVIPLMERIGDLWRKGEIRVS
                     HEHLATQAVRTFLGNLLATQKTFPGAPHVLVTTPSDQVHEIGALSAAVAAASEGWNVT
                     YLGPSLPSEEIAGAVTHNGSRALILSLIYPESDPLVARELRKLRRYLPGMPVIVGGRA
                     RQSYSQVLTEIEAVVVNDLYDVRTVLESIRTTGGI"
     gene            complement(6644..7483)
                     /locus_tag="VFJ67_09620"
     CDS             complement(6644..7483)
                     /locus_tag="VFJ67_09620"
                     /inference="COORDINATES: protein motif:HMM:NF037752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7290682.1"
                     /translation="MLNIIKLTAVTVFIYLLSAAASHAVVQLVDTENSGDRINVDNNI
                     TSGFIATGSGFGDDFEVIMCGTYSDGSNLFLPPAPDGWTTLNTGECGGSEQCILGIYG
                     RFDSSPGSMDITCSWSDPTDAFSAGSFRYFGVDRFDPIIDVSCVTGGEQTPVTTPPVD
                     AEPGSAVILAVQLGNQGSEMFSVSPQAEPILQGDFFAVGGSEFGEQSLTLGFSILVPT
                     GESIPEIPIFPPGNYDWRTCAIVLRAAPASIPTLSEWGLGAFAALFGIAAVWALRRRT
                     ARA"
     gene            complement(7536..>8237)
                     /locus_tag="VFJ67_09625"
     CDS             complement(7536..>8237)
                     /locus_tag="VFJ67_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HET7290683.1"
                     /translation="GLPGDFEVLICSTVSDGSNSFLDPAPDIWSAIDTGDCAGGICIL
                     GIFGRFDQSPDSSAITCNWTEPTSAVAALSFRYRGVDGDNPVLDVQCSVGNADDQLII
                     PSVNTEPGSAVLLAYTFGFNPNSTKGSPGINESLAADPIFQGGNAAVGQELSQPQEVI
                     TAGQSLIYRSGGPTGDFNFGIAGLEWRACAISLSPGPVNIPALSEWGLGAFAVLFGIA
                     AVWALRRQAARVYRK"
BASE COUNT         1701 a         2398 c         2213 g         1925 t
ORIGIN      
        1 catgagaagg agcttgttgt cggtgtggaa attcacgagc gcgtttgcgg tccccacctt
       61 cctcgcttcc ctgaacaggg cgagcgtgta gagctttgta aggaaatgct ccctgattct
      121 gaagttcctg agacgcccct cgtcctcgat ggcgagaccc gggaagcgtc cgatgacggc
      181 ctctgcgaag aacccggccc ccttgcccgc ggctatctcg ctcacggcgt ccgggaactc
      241 cttcgcgtcc ccgagcccgc acgagggcga gcggcttttg agaatgaacc cgtccgcgtc
      301 tccgagcgtg tcgagatacc tcgccgagaa ttcccgcatg agcgaggtca ggtctctccc
      361 cgtcgagggc tggacgagct tcctctctcc gcccttactc acgatcctca ccgggtcgcg
      421 cggcactccg agccctatct ccagctcggg acataccggt ctgagctcga cccagggttc
      481 taatgcctgg acgaagttgt tggggatcat ctgcccgttg tacctgcaag cgtcgaattc
      541 gaaacacctg ctcgtgacga ctacgggttt gggaaatgtc ctcatgaagg gtttttcttc
      601 cttctctttc actggagcgt tgtcgtcggc ttctcagcgt cgtccgagaa ggagcggttt
      661 atcccctccc acctggagag aagcatcgcg agcaccttct cggcgtcctc cgcgctgccc
      721 tcgcgggcga gcgtgagcac gagcctgagc tcggaaacgg agtcctcgtt cttaatctgg
      781 tccgggtcta tgcgcgagct catctcgccg taattcagca gaacgaagct ctcccggtca
      841 aaacccccga gccagtgcgt gaggctctct cctccggcct tcaagtcgct tagtatcccg
      901 gcgagcgccg ggcttccggc ggattcggga gacgcgagga acgcgtcaat gtcgctcacc
      961 gccgcttcca gcctttcgag cgcgctcccc actctcgtta tgtaatagat cgagtcctgc
     1021 cttatcgcgc cgaagccggt gtgcgtgaag aaatacttct tctcggcgcc ctcgtacatg
     1081 aggaaccatt cgagcggtat gaagttctcg gcgctgaata tgttggggtt ggccttctgg
     1141 ccgacgggcg tgacgtagta ttttccgtcc tcgtccttca tgaagaagca gtcctcgatg
     1201 aggcgcttct ctatgccgag ctccttggac agggattccc tgaacgcgtc gtactcgctc
     1261 ccgtaccagg ccgtgttcgg ggccgccggg ccgtccttga acctctcgta gaacgatatg
     1321 tagctccgcc agtaatcgta acgctccccc gtcttttcga ggggctcgta tgtcatgagc
     1381 acgcttaaag ccatctcagc ctctcactta gtttagcaaa ttgccggtaa aatccctagc
     1441 ccgtggggaa taggagggtc aggtggggag accggtgtcc ttctttatct gcgagatgta
     1501 ctggtcgtcg atgcgtccgc cggagtatgc ctcgtgcgcg aggttcctca tctcgtaatg
     1561 gttcacgatg ggcctcgggt aggggagctt gtaagtgagc gggttgtgta tcttctccgg
     1621 gtcgacggcc gagaggtcgg ggatgtattt ctttatgtat aaacattccg ggtcgtagtt
     1681 ctgcgactgg agtatggggt tgaatatgcg gaagggcttc gggtctgctc cgcacgaggc
     1741 cgaccactgc cagttgccga tgtccacggt ctcgtcgtaa tcgaccaggt gctcgaagaa
     1801 gtgcctgtcc ccgagcctcc agtcggtgat gaggtccttt gtcaggaatg aggccacgat
     1861 catcctcacc ctgttgtgta tccatccctc ttccctgagc tgcctcatcc ccgcgtccac
     1921 tatcgggtat cccgtccgcc cctccctcca tgcgtcgtac cagggctcgc tgtttatcca
     1981 ctttatgttg cgccgcttct cctggaactc gagccccctc gtctccggga agttgtgcat
     2041 tatgtggtac cagaattccc tccatgcgag ctcggaaatg tatgagcccg ggtcggacga
     2101 cgcccttgtt gcggcctcgt acacccttct cacggacact atgccgaatc tgagatatgg
     2161 ggagagcctc gacgtaccgt ccagataggg gaagtccctc gtcatgccgt aatccccgaa
     2221 gttgaaatcc ctgagcctcc tcgccgcgaa atcgatgggc cagtatatat tcccggcccc
     2281 gcccaggtcg cgcaggacgt tttcggccgg gctcgcggtg accgccgggg agtttatctt
     2341 cccgggtgcg tcgagcgcgg gtctcttcgt cttcccctgc caaatgccgt agaacggggt
     2401 gaacaccttt ctctggtcta tctcgtgagg cgggacgagg aacgtgtcgt cgtattttct
     2461 gaaagtgacg tcgtggagcc tgcactggtg ctcgaccccg aggtcgcgct tcacgcccga
     2521 gaagccgtaa gccctgttcg tgtacacggc ctcgaccttc tgtgagccga tgagcgaggg
     2581 gattatctcc tcggccttgc cccttagaac gagcaggtac gagcctaatc tcctgagctc
     2641 cgaatcaagt ttcaagagag cgtccgcgag aaacccgagc ctcttgctgc cggacgagta
     2701 tttcgagagt atggagtctt cgagaacgta gagcggtatg acctccctgc tgtccctgac
     2761 agcgttcaga agccccgtgt tgtcgtccgt gcggaggtcc cgcttgaacc agaaaatagc
     2821 cttgccgtga atcatccgct ctgttccatg cctccttgat cgatcttcgg ttaaatgacg
     2881 cactcactga gggagcgctt tacccccttt cgtgaggtga gtccggcggt gcggtgtgca
     2941 cagattcaat tctactctgt aactatcgcg gattctacta aaaattcggc tcaggcacac
     3001 aatgtgtgct gatatacgat tcattccggc ccctctgctc aaaccggcgg ctaaaatccg
     3061 ataccgttca tcctgagcct gttgaaggat gcaaacacag tgtacggcga ggaacctgcg
     3121 gcccgcattg tgcggctcgg aacgcgcaca tttacgatct gtacaaatat cttccgcttg
     3181 taggaacgag ctcctgaata gatcctggaa taaattcagg gcatggttca ggacaggccg
     3241 gccgcggcat cttatatatc cctcccattt ttatgaatgg ggagggagtg ggtgggggtt
     3301 tacattaatc tatctgtcat tgcaacgatt gcgatgaata agagcaatcg gacgaccttg
     3361 gctgattacg aataagcacc gtaaccttcg taccgcagtt tcaaaaacca aattatgcag
     3421 cgaagcagcg cggcaatctc atgaataaag caagaccccc gtgtttactc tcttaaggct
     3481 catggccgat acggcgggag ttccttcaat aagcggcgta ttgctacaat ttaaagccgg
     3541 ggctatcccg atatccttac agatagccct tcagggtatc gcccaggccg gatttcgata
     3601 tcttctcgag agcggccttc tctatctgcc ttatcctttc ccgggtgacg ccgaacttgt
     3661 tccctatttc gtcgagagtg tacaccttct cgtcgccgag gccgtatctc atcctgacta
     3721 tctcctcttc cttcggggtc agggacgaga ggcattcctc gaggagactc tttaccctta
     3781 tgtcggaaac ggcggcttcc tgtccctccc tcgagtcggg catcaggtca acgtaagtcc
     3841 tctcgttatc gtcagccacg ggattatcga gcgagagcag actgtccgtg ccgtcgagca
     3901 cggcctgcac tacctctctg gacaagccgg ctttctcggc tatctcttcg gggtagggct
     3961 tcctgccgag ctcctcttcg agcgcgtact tcgctctgaa caccttgcct gactgttcga
     4021 gcacgtaaac ggggaccttg atagtcctcg tcttctcggc tatagccctc gtgagcgcct
     4081 gctgtatcca ccacgcggcg tatgtcgaga acttgaatcc cttcctgtgg tcgaacttat
     4141 cgaccgccct catgagaccg aggttcccct cctgcacgag gtccgtgagc gggagccccc
     4201 ttccgaggtg tctcttcgct atgctaacga cgagcctgag gttggatctt atgaatttcg
     4261 atttgagctc ctgcgccttt tccgaaaaga cctttctcgt ggcgtcgagc ctgtttttat
     4321 ccccggcctt tttgccgagc ctggcccggg taagccgctc gatgtccttc tggatcatcc
     4381 tcgccttctt ctcgcattcc tttatcttgg ccccgagctg cttctcgtca tcaggggtaa
     4441 gcaacggctc cgtcaacagg tcacggtagt acacttggag gactctttcc tcttcgtact
     4501 tggtccattt cctctccttc cccttccgct tccctgcggg ggagacggcc gggcttccct
     4561 cagtatccgc gaacgaattg ccggagtttt ccccctttcc gccccggacg aagaactcat
     4621 cgtcctggta gccttcttcc gagaaataat ttttatcgaa ttcgttgtcc atcataattg
     4681 gaataccccc ccactgcttc tttatactct ttacatattc cttatatatt cctttagaca
     4741 taatttacaa gggccgggat tcatccggcc ctgaaattaa tgtaaatttt agtcggccgg
     4801 aggcatcata accgtatcga tcacgtgtat gacgccgttt tccgccatta cgtcggtctt
     4861 tataacggtc gccccgtcaa ccatgacctt cccgtcggag gtctttatgt ctatcttctt
     4921 gccgctcacg gactccgccg agctcagctt gaccacctcc tcagccgtaa cctttccgga
     4981 cacgacgtga taggttagta tggccgcgag cttttccttg ttctcgggct tgagaagcat
     5041 ttcaagcgtg ccttcaggga gctttgcgaa agcttcgtcg gttggagcga agacggtgaa
     5101 cgggcccact cccttaaggg tttccgtgag acccgctgcg ttcaccgcag ccacgagtgt
     5161 gttaaacgaa ccggccgcag ccgccgtctc cactatgtcc agctcttcca cggcctcagc
     5221 gtcagtgaac gggactttcg tgcttaaccc cataccggcc gcgatcaaca aaaccaaaaa
     5281 ccatctcgag atgataaatg acctcatatt tgaaacctcc tgagatttat tgaatacccc
     5341 cttactttta actgcgtccg acgccgatgt ttccgttcgc cagagatacg gaacatcgtg
     5401 tctagtagtt gtcaaatata ataacaggat gtctaacatg tgtcaagcat attttgcaaa
     5461 aatatttttc ggaatttttt gaagtgaatg gtcttaacgg tgttgggttt acagttatat
     5521 tgttctcgtc aggggagtta gagattgttc ttaaattaaa cagaaaacat attgaaatcc
     5581 ggatattaat atttgaataa tccttgacat atattagaca aaagtataac attatgtcca
     5641 tgcaaggccc cggcgaggaa acggataaga gattttcgat gaaagtcgcg tccagaatga
     5701 gcggactgac cccgcacgcc ataagggcgt gggagagacg gtacggggtc gtgaagccgg
     5761 gaagggactc caataacagg aggttctacc gggagagcga catcgagagg ctcaccctcc
     5821 tccacaaggc gaccgacgcc ggacacccga taagccagat cgccgggtac tcgaaggagg
     5881 agctgagggg tatagtcggc agtatgaacg tcatagacct gccgtcgagg aagcccgcgg
     5941 agatgcgcag caccggggac cttaccctcg tcagggacta caacgcatac gtcgaggaga
     6001 taatcgcggc agtggagaag atggacagaa agtcgcttga ggaaacgctc atttcggtcg
     6061 agacggagat aggccggaac gcgctcctcg agcgcgtcgt catcccgctc atggagagga
     6121 taggggacct ctggaggaag ggcgagatca gggtatcgca cgagcacctg gccactcagg
     6181 ccgtgaggac gtttctcggg aacctgctcg caactcagaa gaccttcccc ggcgcgccgc
     6241 acgtcctggt aacgacccct tccgaccagg tgcacgagat aggggccctt tcggcggccg
     6301 tcgctgcggc gagcgagggg tggaacgtca cctacctcgg gccgagcctg ccttcggagg
     6361 aaatagccgg cgctgtaacg cacaacggct cgagggcgct catactgagc ctgatttacc
     6421 ccgagagcga cccgctggtc gcgcgcgaat tgcggaagct gaggcgctac ctgccgggca
     6481 tgcccgtaat agtcggcggg agggccaggc agagctactc gcaggtgctt acggaaatag
     6541 aagccgttgt cgtgaacgac ctctacgacg tccgtacggt gctcgagtcc attagaacaa
     6601 ccggtggaat ttaataaccg gcgggtgaca atattctttt tagttaagcc ctcgccgtcc
     6661 gcctccttag cgcccatact gcggcaatgc cgaacagcgc ggcgaatgcg ccgagacccc
     6721 actccgatag cgtaggtatg ctcgcgggcg cggcccttaa tacaatggcg caagtcctcc
     6781 agtcataatt acccgggggg aaaataggta tctcgggaat agactcaccg gtcgggacta
     6841 aaatgctgaa ccccagtgta agtgactgct cgccgaattc gctgccccct actgcaaaaa
     6901 aatcaccctg aaggatcggc tctgcctgag gcgatacgga aaacatctcc gatccctggt
     6961 taccgagctg aacggccagg ataacggccg agccgggctc cgcatccacc ggcggcgtcg
     7021 tgactggtgt ctgctcgccg cccgtgacac atgacacatc aattatcggg tcgaaccggt
     7081 cgaccccgaa atacctgaaa gagccggccg agaacgcgtc cgtcggatca gaccagctgc
     7141 atgtgatatc catggaaccg ggcgagctgt cgaacctccc gtatatcccg agtatgcatt
     7201 gctcgctccc tccgcattcg ccggtattca gtgtggtcca gccgtccggg gcgggtggga
     7261 ggaacaagtt gcttccgtcg gagtaagtac cgcacattat gacttcgaaa tcgtcaccga
     7321 agcccgagcc tgtcgcgata aacccgctcg tgatgttgtt atctacgttg attctgtcac
     7381 cgctattctc tgtgtcgacc agctgaacga ccgcgtgaga agcggccgcg gagagaagat
     7441 atataaatac cgtaactgca gttaatttta ttatatttaa catcgccatc ctccttaatc
     7501 ggaatttttt atatcgatgt tattcgtaat atgaatcact tccgatatac cctcgccgcc
     7561 tgcctcctga gtgcccatac ggccgctatg ccgaagagca cggcgaatgc gccgagaccc
     7621 cattcggaaa gcgccggtat attaaccggc ccgggactta acgatatcgc gcacgccctc
     7681 cactccaaac cggcaatgcc gaaattgaaa tcgcctgtag ggccgcctga ccgatatata
     7741 aggctttggc ccgcggtgat gacttcttga ggctgcgata attcctgacc gacagcagcg
     7801 tttcctccct ggaatatcgg atcggccgca aggctttcat taatacccgg actaccctta
     7861 gttgaattcg gattaaagcc gaacgtataa gctaatagaa ccgccgagcc cggttctgta
     7921 ttgacagagg gtatgataag ctgatcatcg gcgtttccca cggaacactg aacgtccagg
     7981 accggattgt cgccgtccac gccacggtat ctgaaagaca acgccgctac ggcgctcgtc
     8041 ggttcagtcc aattacacgt aatagccgag gaatccgggg attggtcgaa tctcccgaat
     8101 atgccgagta tgcagatgcc gccggcgcaa tccccggtgt cgatagcaga ccagatgtca
     8161 ggagcgggat cgagaaatga attgctgccg tcagaaaccg tcgagcaaat caagacctcg
     8221 aaatctcccg ggaggcc
//
LOCUS       DASSJH010000437         7412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1286957, whole genome shotgun sequence.
ACCESSION   DASSJH010000437 DASSJH010000000
VERSION     DASSJH010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286957"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1897
                     /locus_tag="VFJ67_09630"
     CDS             353..1897
                     /locus_tag="VFJ67_09630"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET7290684.1"
                     /translation="MKDKIAVLEEKEREAEAGGGEARIQKQHEIGKLTARERIDLLLD
                     KNTFIELDKFVVHRCSDFGMDSKKFLGDGVVTGYGKIDGRQVFVYAQDFTVFGGSLGM
                     VHGRKICKVMDLAMQVGAPIVGLNDSGGARIQEGVESLGGYGEIFCRNVLASGVVPQI
                     SAIMGPCAGGAVYSPAITDFTLMVRNTSYMFITGPDVIKAVTHEEVNSEELGGAIVHN
                     SRSGVAHFAVEDDKECIVLIKELLSFLPSNNMEDPPRHPTDDPSDRRATALRAIVPEN
                     PNKPYDVKDVINAVVDDGYFFEVQEHYAQNIVIGFARMAGRVIGIVGNQPNVLAGCLD
                     IDASVKAGRFVRFCDCFNIPILTFEDVPGFLPGTSQEWGGIIKHGAKLLYAYSEATVP
                     RVTVITRKAYGGAYDVMSSKHVRGDIVMAYPTAEIAVMGSDGAVNIISRAQIMQAEDP
                     DAERAKLIEEYKERFANPYRAADLGFVDEVIRPEDTRPRVISAFEMLDGKRQTNPPKK
                     HGNIPL"
     gene            1915..3408
                     /gene="accC"
                     /locus_tag="VFJ67_09635"
     CDS             1915..3408
                     /gene="accC"
                     /locus_tag="VFJ67_09635"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET7290685.1"
                     /translation="MFNKILIANRGEIAVRIIRACRDLGVGTVAVYSDADRRSLHVAL
                     ADEAYHIGGSAPGESYLNKEKIIEAAKKSVAEAIHPGFGFLSENESFAELCEKSGIVF
                     IGPSPEAIRLMGDKITARKIAHDAKVPLVPGSDGAVSDVEASVIADEIGYPVMIKASA
                     GGGGKGMRLVRSREEFESSLRMARSEARSAFGDDSVFVERFVERPRHIEIQVIADSEG
                     NTLYLFERECSIQRRHQKVIEEAPSPAISARTRTKMGEIAVRIAKAVKYRGAGTIEFI
                     MDQSQNFYFLEMNTRVQVEHPVTEMITGFDIVKWMIRIAAGEKLPFKQKSIAMKGHAL
                     ECRVYAENPETNFLPSPGVIEYLRAPSGPGIRDDSSIYNGCEITPFYDPMLSKLIVWA
                     DTREAAIRKMESALREYIVLGVKTNIGFLIRVMNNEEFRKGKIDTGFIERHPELLKPP
                     VLEIEPALITAALSMSSAESAAQTAQKPASNWKLFGRRSGVSRSPFA"
     gene            3405..3899
                     /locus_tag="VFJ67_09640"
     CDS             3405..3899
                     /locus_tag="VFJ67_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET7290686.1"
                     /translation="MTYTFKFRDQIYKIALEKEDGQLQAEIEGNRVPVDFERVENNLY
                     SMLVNGKSMTVGVVRKGRKIQVFIDGDLYELEAVSEREQRKTSHIVSGVQEIKSPMPS
                     RVVKILKAEGDEVTPDEGLIVVEAMKMESELKSPIAGKIRNIMVKEGDAVESGTVLLV
                     VSSE"
     gene            complement(3908..4801)
                     /gene="rsmA"
                     /locus_tag="VFJ67_09645"
     CDS             complement(3908..4801)
                     /gene="rsmA"
                     /locus_tag="VFJ67_09645"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET7290687.1"
                     /translation="MTDRRRKEFDPREFFHGEKAFPRKSLGQNFIKDRRVIDRIVESA
                     ELSADDCVLEIGPGMGALTPALAEKSGRVIAVEKDERVIAPLLKLLDPYPNTEVVRAD
                     CLKLDFRDFYSGKKLKAVSNLPYSISTPVLIKLLGDRDIFSSLVLMLQLEVGQRIIAR
                     PGGRQYGSLSVLIQTYMDVKILFRVPPSAFWPKPKVDSVVLKFVPLPGPRVPLSDERI
                     HERVLRAAFSSRRKMLGNSLRSLLPKEAAESVLAACGIDRSRRAETLPIGEFGLLANE
                     VARSHKAELTPDLSLSPTTVW"
     gene            complement(4907..7042)
                     /locus_tag="VFJ67_09650"
     CDS             complement(4907..7042)
                     /locus_tag="VFJ67_09650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7290688.1"
                     /translation="MKKYTDLKNKITYSNFLLGKLFAVLLSALILILLYKNSFHTLNF
                     YSALASLLLISTAGYRTLNLVKAGNQNIPVPERLELSILYTLVFEIVLEIFGQDIFPL
                     SFLMVPVLIYCFGWPGGVLSLLIVSVLRVTQHFGAGALVQVSLLSITALVIGYLLKGG
                     RERLGSYIFQRNRGKNIILPALISGTVPKDENNEPLSLKELKSEIRNSLNILCDLVPN
                     HSVVLYMKMDDGLFAMTDFISKSRDYVDRGQRLNFRGGYLGWVLKTRTQVLITSIKNV
                     RKNLVYYNKNVPIKSLLATPLFFKGQRVESEGDVSIFGLLVVDSLQQDAFGEKEKLIV
                     SLLSDRIVETIDRYLLSEKVNLSSQELNSFYDFTQKLNSTSDIEVILDHVIDTLLRLF
                     EADFVGISLVDKEGGYSYLKRVSEGMKEHLTDKEIPHGDTLVGLVTESGNHFHFDDIT
                     ARAKYRSIFGKEIDFALGIKNIKSVLIYPFHETYQDVEYKDQNVLGCVVIGRRTKSPF
                     NEGEISLAKIICRESAKSMTSSLNFQKVKELAIRDGLTGLYNHRHFQEMLTYTIHHAD
                     RFSTEASLLLLDVDDLKIINDTYGHQAGDAVLSSIGSTLGESLRKVDIPARYGGDEFA
                     VILPNTGKSGSIAVADKIRSRLKKVSLKSDSREVAVTFSIGIATYPQSASGKELLVEK
                     ADRALYESKNHGKNKITHYEDISLEELGS"
     gene            complement(7014..7283)
                     /locus_tag="VFJ67_09655"
     CDS             complement(7014..7283)
                     /locus_tag="VFJ67_09655"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET7290689.1"
                     /translation="MEKVIFRVILILAVVSFLVYLFAREISQVYALHKENDKVKLEGK
                     ELEMANNELRKKIELIKTDKIFMEKIIREELGMIKTGEKIYRFKE"
     gene            complement(7284..>7412)
                     /locus_tag="VFJ67_09660"
     CDS             complement(7284..>7412)
                     /locus_tag="VFJ67_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7290690.1"
                     /translation="SIKTKLMVLPDTYKVHPGHGPSTTIGMERRSNPFLTGDLIIL"
BASE COUNT         1832 a         1946 c         2066 g         1568 t
ORIGIN      
        1 ggtctcgaga ctgagggcgc agatgccggc tgtggaaaaa gccagacacg gagactacgt
       61 catcgacaac tcgggccttc ccgaagatac gcggagacgc gtgaaggaag tatgggaggc
      121 tatttgctca ccggggaaca gaacagcctg acggttaata aacctgaacc agcctggatt
      181 cggtattaca tccgcaccgc acacgcccga ttcaaccgca cgaaaacacc cgataaaatt
      241 ccttatattt tcgggcaata cacacgaaaa ccgcgcctga ctctcaaact tgccaattag
      301 gtttttacgt agtatttttt tggattctca agaaccttca aaggagccga taatgaagga
      361 caaaattgcc gtactcgaag aaaaggaaag agaggccgaa gccggaggcg gcgaagcgag
      421 gatacaaaaa cagcacgaaa tcggcaaact taccgcaaga gaaagaattg acctgctact
      481 cgataaaaac acattcatag aactcgacaa attcgtcgta cacagatgca gcgatttcgg
      541 catggactca aagaagttcc tcggggacgg ggtcgtcacc gggtatggca aaatagacgg
      601 acgccaggta ttcgtctacg cccaggactt taccgtcttc ggcggctcgc tcggcatggt
      661 ccacggcagg aagatatgca aggtgatgga cctcgccatg caggtaggcg cgcccatagt
      721 cggacttaac gattccggag gcgcccgcat acaggagggg gtcgagagcc tcgggggtta
      781 tggggagata ttctgcagga acgtgctcgc ttcaggggtc gtgcctcaga tctccgcgat
      841 catgggacct tgcgccgggg gcgctgtgta ctcgcccgcg ataaccgatt tcacgctgat
      901 ggtcaggaac acgagctaca tgttcatcac cgggcccgat gtgataaaag ccgtgacaca
      961 cgaggaagtg aattcggaag agctcggcgg cgccatagtg cataattcga ggagcggggt
     1021 cgctcatttt gccgtggaag acgacaagga atgcatcgta cttataaaag aactactgag
     1081 cttcctccca tcgaataata tggaagaccc tccgagacac ccgactgacg acccttccga
     1141 caggagggcg accgcattga gagcgattgt ccctgaaaac ccgaacaagc cttacgacgt
     1201 caaagacgtg ataaacgcgg ttgtggacga cggctatttc ttcgaggtgc aggagcacta
     1261 cgctcagaac atagtaatcg gattcgcgcg gatggcaggc agggtcatag gcatagtagg
     1321 gaaccagccg aacgtgctcg cggggtgcct cgacatagac gcatccgtaa aggcggggag
     1381 gttcgtgagg ttctgcgact gcttcaatat acccatactg acgttcgagg acgtacccgg
     1441 cttcctgccc ggcacatcgc aggagtgggg cggaatcata aagcacggcg ccaagctcct
     1501 ctatgcctac agcgaagcga ccgttccgag ggtgaccgtt ataacgagga aagcctacgg
     1561 cggggcgtac gacgtcatgt cgtcaaagca cgtgagggga gatatcgtga tggcgtatcc
     1621 gaccgcggag atcgccgtca tgggctcgga cggcgccgta aatattatct cgcgtgccca
     1681 gataatgcag gccgaggacc cggatgcgga gagggcgaaa ctcatcgagg aatataagga
     1741 gaggttcgct aacccttaca gggccgcaga cctgggcttc gtcgacgaag tcatcagacc
     1801 cgaagacacg agaccgaggg tcatctcggc gttcgagatg ctggacggga agagacagac
     1861 gaacccgcca aaaaagcacg ggaacatacc actctaacag caaaggatta ttgaatgttc
     1921 aataaaattc tcatagccaa caggggagaa atcgcggtaa ggatcatacg ggcgtgccgg
     1981 gacctcggcg taggcacggt cgccgtatac tccgacgccg acagacgatc cctgcacgta
     2041 gcgctcgccg acgaggcgta ccacatcgga ggttccgctc cgggcgagag ctatctaaat
     2101 aaagagaaga taatagaggc cgctaaaaaa tcagtcgccg aagccataca ccccggattc
     2161 ggattcctct cggagaacga atcattcgcc gagctctgcg aaaaatcggg catcgtgttc
     2221 atcggtccct cgcccgaagc gatacggctc atgggggaca agataacggc gagaaagatc
     2281 gcccacgacg cgaaggtgcc gctcgtgccc ggctcggacg gcgctgtgag cgacgtcgag
     2341 gcgtccgtaa tagccgacga gatcggctat ccggtaatga tcaaggcctc agccggaggg
     2401 ggcggaaaag ggatgaggct cgtgaggagc agggaagagt tcgaaagctc gctccgcatg
     2461 gctaggagcg aagcccgctc cgctttcggc gacgattccg ttttcgtcga aaggttcgta
     2521 gagcgcccga ggcacataga gatacaggtc atcgccgata gcgaaggaaa cacgctctat
     2581 ctcttcgagc gcgagtgctc catacagaga aggcaccaga aggtgatcga agaggcccct
     2641 tccccggcca taagcgcaag gacgcggacg aagatggggg aaatcgcggt gaggatcgcg
     2701 aaggcggtta aatacagagg ggcgggaacg atagagttca tcatggacca gagccagaac
     2761 ttctacttcc tcgagatgaa cacgagggtc caggtggagc acccggtgac cgagatgata
     2821 accggtttcg acatagtgaa atggatgatc aggatcgccg caggagaaaa gctcccgttc
     2881 aagcagaaaa gcatcgcgat gaagggacat gcgctcgagt gcagggttta cgccgagaac
     2941 ccggagacaa acttcctccc gtccccgggc gtgatcgaat acctgagggc tccgagcgga
     3001 cccggcatca gggacgattc ctctatatat aacggctgcg agatcacgcc gttctatgac
     3061 ccgatgcttt caaagctcat agtatgggcg gacacgagag aggcggccat aaggaagatg
     3121 gaatcggcgc tgcgcgagta tatagtgctc ggcgtcaaga cgaacatagg cttccttatc
     3181 cgcgtgatga ataacgagga gttcaggaag ggaaagatcg acaccggttt catcgaaagg
     3241 catcccgaat tgctcaaacc ccccgtcctt gaaatagagc ccgcgctgat tacggctgcg
     3301 ctttcgatga gcagcgccga gagcgcggcg cagacggcgc agaaaccggc gtcgaactgg
     3361 aagctcttcg gcaggagatc aggcgtatcg aggagcccct tcgcatgacg tatacattta
     3421 aattccgaga tcaaatctac aagattgcgc tcgaaaaaga agacggccag ctgcaggcgg
     3481 agatagaggg caacagggtc ccggttgact tcgagagggt agagaacaac ctctactcga
     3541 tgctcgtgaa cgggaagtcc atgacggtgg gggtcgtaag gaaggggagg aaaatccagg
     3601 tctttataga cggcgacctc tacgagctcg aagccgtttc cgagagagag cagcgcaaga
     3661 catcccatat cgtctccgga gtccaggaga taaagtcgcc catgccgagc agggtcgtga
     3721 aaatactcaa ggccgaaggg gacgaagtca cgcccgacga aggactcatc gtggtcgaag
     3781 ccatgaagat ggagagcgag ctcaaatccc ccatcgccgg aaaaatcagg aacataatgg
     3841 tgaaggaagg ggacgcggta gaatcaggga ccgtgctcct cgtagtctcc tccgaataag
     3901 gaaacaccta ccaaaccgtt gtcggcgaca gggataggtc tggagtgagt tcggctttat
     3961 gactccgcgc cacctcgttc gcaaggaggc cgaattcccc gatgggtagg gtctcggccc
     4021 gtctcgacct atcgattccg cacgcggcca gcacactttc ggcggcttcc tttggaagaa
     4081 gcgaccgcag cgaattaccg agcatcttcc tcctggatga aaacgcggcc ctcagcaccc
     4141 gctcgtgtat acgctcgtcc gagagaggga ccctcggccc gggaagaggg acgaatttga
     4201 ggacgaccga atcgactttg ggctttggcc agaacgccga gggagggaca cggaatagaa
     4261 tctttacatc catgtacgtt tgaatgagca ccgacagtga gccgtactgc ctgcctcccg
     4321 ggcgggcgat aatcctctga ccaacttcca gctgaagcat gaggacgagg gatgaaaaga
     4381 tgtcgcggtc tccgagaagc tttatgagaa cgggagtcga tatgctgtac ggcaggttgg
     4441 agactgcttt cagctttttc ccactgtaaa aatccctgaa atcgagcttg aggcagtcgg
     4501 cccggacgac ttcggtattc ggatagggat caaggagctt aaggagaggc gctatcacgc
     4561 gctcgtcttt ctctacggcg atgactctcc cggacttttc cgcgagtgcg ggcgtgagcg
     4621 cccccatgcc gggacctatc tcgaggacgc agtcgtccgc cgaaagctca gccgactcga
     4681 cgatcctgtc tatgaccctt ctgtctttta tgaaattctg accgaggctc tttctcggga
     4741 acgccttttc gccgtggaaa aattccctcg gatcgaactc ttttctccgt ctgtccgtca
     4801 tttatcgccg tgagatatat atatggggaa ttataacttt aaacggtccg ctccgcacaa
     4861 aaaggagaac cagagacgac gccgggcctg tgcctatcga cctgactcag ctcccgagct
     4921 cttcgagaga tatgtcctcg tagtgcgtga tcttgttctt gccgtgattc ttcgattcgt
     4981 aaagcgcgcg gtcggctttt tcaacgagca attccttacc gctcgcggac tggggatatg
     5041 tggcgatccc gatgctgaac gttacggcca cctcgcgcga atccgatttg agggagacct
     5101 tcttcagtct gctccttatc ttgtccgcga ctgcaatgga gcccgacttg ccggtattgg
     5161 gcagtatcac ggcgaactcg tctcctccgt acctggccgg tatgtcgacc ttcctcaacg
     5221 attccccaag cgtgctcccg atcgacgaca atacggcgtc gcccgcctga tgaccgtacg
     5281 tgtcgttaat tattttcaag tcgtccacgt cgagcagcag aagcgaagcc tcggtcgaga
     5341 acctgtcggc atggtggatt gtataagtga gcatctcctg aaaatgcctg tgattgtaga
     5401 gcccggtgag cccgtccctt atagcgagct ccttcacctt ctggaaattg agggaagacg
     5461 tcatggactt ggccgactcc ctgcatataa tcttcgccag gcttatctct ccttcattga
     5521 aaggggactt cgtccttcgg ccgatgacga cgcagccgag aacgttctga tccttgtact
     5581 cgacgtcctg atacgtctcg tggaatgggt agatgagaac ggactttatg ttctttatcc
     5641 cgagggcgaa atctatttcc ttgccgaata tgctccggta cttcgccctc gccgttatat
     5701 cgtcgaagtg gaagtggttg ccgctttcgg ttacgagacc aacgagcgta tcgccgtgcg
     5761 gaatctcctt atccgtcagg tgctccttca tcccttcgct caccctcttg agatagctgt
     5821 agccaccctc cttatccacg agcgatatgc ctacgaaatc ggcctcaaac agcctgagca
     5881 gagtatcgat tacatggtcg agtatcactt ctatatcgct cgtcgagttg agcttctgcg
     5941 tgaaatcata gaaggaatta agctcctggg agctcagatt gactttctcg gacaggaggt
     6001 acctgtctat ggtctcgact atcctgtcag aaaggagcga tacgatgagc ttttcctttt
     6061 caccgaaggc gtcctgctgg aggctgtcga caacgagcag gccgaatatg ctcacgtccc
     6121 cttcggattc gaccctctgg cctttaaaaa aaagaggcgt ggcgagcagg gactttatcg
     6181 gtacgttctt attatagtaa acgagattct tcctcacgtt cttgatgctc gtaatgagca
     6241 cctgggtcct ggtcttgagg acccagccga gataccctcc ccggaagttg agcctctggc
     6301 ccctgtccac gtaatccctg ctcttcgata tgaaatcggt catggcgaag agcccgtcgt
     6361 ccatcttcat gtagagcacg acactgtgat tcggaaccag atcgcaaagt atgttgaggg
     6421 agttccttat ttcgctcttg agctccttaa ggctgagagg ctcgttattt tcgtctttcg
     6481 ggactgtccc cgatatcagc gcaggcagga ttatgttctt gcccctgttc ctctggaaga
     6541 tgtatgagcc caggcgctcc ctcccgccct tcagcaaata cccgatcacg agagccgtaa
     6601 tcgagagcag cgagacctgg accaatgcgc ccgctccgaa atgctgagtt acacgcagga
     6661 ccgagacgat gagcaaagac agtaccccgc ccggccatcc gaaacagtaa atcaggaccg
     6721 ggaccatcag gaatgagagc gggaaaatat cctgcccgaa tatctccagt acgatttcaa
     6781 aaacaagggt gtagagtatg gagagctcaa gcctttcagg aacgggtatg ttctggttgc
     6841 ccgctttgac cagattaagc gtcctgtagc cggccgtcga aatcaggagt aggctcgcca
     6901 gggcggagta aaaattgagc gtatgaaagc tgtttttata taataggatt aatatgagtg
     6961 cggagagcag gacagcgaat aactttccga ggaggaaatt cgaatatgtg attttattct
     7021 ttaaatctgt atattttttc accggttttg atcatcccca gctcttctct tattattttc
     7081 tccatgaata tcttgtccgt ctttatcaac tcaatctttt tcctgagctc gttattagcc
     7141 atttccaatt cctttccttc aagcttcact ttgtcgttct ccttgtggag agcatagacc
     7201 tgagatatct ccctggcgaa caaatacacc aggaacgaaa ctacggcaag tatcaggatc
     7261 actctgaata taactttttc caattataag attatcaaat cgcccgttaa aaagggattc
     7321 gatctccttt ccattcctat ggttgtcgag gggccgtgtc ccggatgcac tttataggtg
     7381 tcgggcagga ccatgagctt cgtcttaatc ga
//
LOCUS       DASSJH010000438         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1071288, whole genome shotgun sequence.
ACCESSION   DASSJH010000438 DASSJH010000000
VERSION     DASSJH010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071288"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /gene="mtaB"
                     /locus_tag="VFJ67_09665"
     CDS             complement(<1..1112)
                     /gene="mtaB"
                     /locus_tag="VFJ67_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HET7290691.1"
                     /translation="MKSISVVTLGCKVNQYDTAAILNRLPRSKYVRVPFPEKADVYVI
                     DTCTVTHKADAEARHYINRARRANPDGVVVVTGCYAQVSPDEIKELTDADYVIGNSHK
                     FSDLLKVIREGETQSEPRLFISDIFREKKKSFETPGIDFFPGRTRAFLKVQDGCNYAC
                     TFCIIPKARGRSRSLPVRDILERMEKLAGAGYKEIVLTGVHIASYGRDIGTSFGELLQ
                     SIEREKIVKRVRLTSLDPADTEPGLIEFIAGSETICPQFHVALQSGDTQVLKRMRRRY
                     GPERFIELTDLIRERIPDAAIGSDIMVGFPGETEEEFTSTYRVAGESALTYFHVFPYS
                     KRRSTPAASMPDQVHPETIKKRSAQLRELGRRKKTE"
     gene            complement(1109..>2161)
                     /gene="mnmA"
                     /locus_tag="VFJ67_09670"
     CDS             complement(1109..>2161)
                     /gene="mnmA"
                     /locus_tag="VFJ67_09670"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET7290692.1"
                     /translation="VDSTTVAALLKKEGHEVIGITMQLLDYGDAEGGCCSLDQVVDAR
                     RAAADLGFPHYVVNFTEEFDKYVLTDYVDKYSSGKTPIPCVLCNKHVKFDLLLRRALE
                     LGADYLATGHYARIDRDSETGLLTLNKALDETKDQTYFLHTLTQKELGRLMFPLGGMT
                     KDEVREIARELGLRQAEKPDSTGVCFVPDGNYRDYLESRSAFTAGKGDIVSTHGDVLG
                     SHRGVFSFTIGQRRGLGIATGKPMYVTRIEPASNRVYVGDEGELYGTKLIAEDITWIN
                     NIHGNDMLDDTLQVEAKVRYRHKASPAELTMWSPSEGTVSFRDPQRAITPGQAVVFYS
                     GDRLLGGGWIKEVLPQ"
BASE COUNT          364 a          677 c          601 g          519 t
ORIGIN      
        1 aattctgtct tctttctccg gccgagctcc ctgagctgag cgctcctttt ctttatagtc
       61 tcgggatgga cctggtcggg catcgatgcc gcgggcgtcg atctccgctt cgaatacggg
      121 aagacgtgga aatatgtgag cgccgattcc ccggccacgc ggtatgtgct cgtgaactcc
      181 tcctccgtct ctccgggaaa gccgaccatt atgtccgagc ctatggcggc gtcgggaatc
      241 ctctcccgta taaggtcggt gagctcgatg aacctctcgg ggccgtagcg cctcctcatc
      301 ctctttaaca cctgcgtgtc gccgctctga agcgcgacgt ggaactgcgg gcatatagtt
      361 tcggacccgg cgatgaattc gatgagcccg ggctccgtgt cggccgggtc gagagacgtg
      421 agcctcacgc gcttcactat cttctcacgc tctatgctct gaagcagctc cccgaagctc
      481 gtgccgatgt ccctgccgta gctcgcgatg tggacccccg tgagcactat ctccttgtat
      541 cctgcacctg ccagcttctc catcctttcc aggatgtcgc gtaccgggag gctccgcgac
      601 ctgcccctcg ctttagggat tatgcagaaa gtgcaggcgt agttgcatcc gtcctgaact
      661 ttaaggaaag ccctggtcct ccctggaaaa aaatcgatgc ccggcgtctc gaagctcttt
      721 ttcttctccc tgaaaatatc cgaaatgaat agccgcggct cgctctgggt ctccccctcc
      781 cttatcactt taaggagatc cgagaacttg tgggagttac cgatgacgta atcggcgtcg
      841 gtcagctcct ttatctcgtc gggcgatacc tgggcgtagc agcccgtgac gaccacgacg
      901 ccgtcggggt tcgcgcggcg cgcccggttg atgtagtgcc tcgcctcggc gtcggccttg
      961 tgcgtgaccg tgcaggtgtc tatgacataa acgtccgcct tctccgggaa ggggaccctc
     1021 acgtacttgc tccgggggag cctgttgaga atcgctgcgg tgtcgtactg gttcaccttg
     1081 cagccgaggg tgacgacgga aatgctcttc attgcgggag cacctctttt atccatccgc
     1141 ctccgagaag cctgtccccg ctgtaaaaga cgaccgcctg gccgggggtt atagccctct
     1201 gtgggtccct gaacgagacc gtcccctcgg aagggctcca catcgtgagc tccgccgggc
     1261 tcgccttgtg cctgtacctg acctttgcct cgacttgaag tgtatcatct aacatatcgt
     1321 taccatgtat attatttatc cacgttatgt cctctgctat gagcttcgtg ccgtagagct
     1381 ccccttcgtc tccgacgtaa accctgttcg aggcgggctc gatacgggtc acgtacatgg
     1441 gcttccccgt ggctattccg aggcctctcc tctgccctat agtgaatgag aatacgcccc
     1501 tgtggctgcc gagcacgtct ccgtgcgtgc tcactatatc gccctttccg gccgtgaacg
     1561 ccgaccttga ctcgagatag tccctgtaat tgccgtccgg gacgaagcac acccccgtgc
     1621 tgtcgggctt ttcagcctgt ctcagcccga gctccctggc tatctccctg acctcgtcct
     1681 tcgtcatgcc tccgagaggg aacatgagcc ggccgagctc cttctgggtg agcgtgtgga
     1741 ggaaataggt ctggtccttc gtctcgtcaa gcgccttgtt gagggtgagc aagccggtct
     1801 cgctgtccct atcgatgcgc gcgtaatgcc cggtcgctag ataatcggcc ccgagctcga
     1861 gcgccctcct gagcaagagg tcgaacttga cgtgcttgtt gcagaggacg cacggaatag
     1921 gcgttttacc cgacgagtac ttgtccacgt agtcggtgag gacgtatttg tcaaactcct
     1981 cggtgaagtt caccacgtaa tgcgggaatc cgagatcggc agcggccctc ctcgcgtcga
     2041 cgacctggtc gagggagcag catccccctt cggcgtcccc ataatcgagg agctgcatcg
     2101 tgatgcctat gacttcgtgg ccttcctttt taaggagcgc cgctactgtc gtgctgtcca
     2161 c
//
LOCUS       DASSJH010000439         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1287173, whole genome shotgun sequence.
ACCESSION   DASSJH010000439 DASSJH010000000
VERSION     DASSJH010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287173"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..782)
                     /locus_tag="VFJ67_09675"
     CDS             complement(117..782)
                     /locus_tag="VFJ67_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET7290693.1"
                     /translation="MGLKKRVGLIVKSQLNDWVSRAEDPEKMLNQAVEEMEEGLERAR
                     VKIGALRFRIDEQEKLSRRLGEQAKYWRERAEDYVRRDMEENAVEAVRKKRLLEEKVG
                     KLALAQSGDRAKLGDFEARYTELDERVRTARTKRTLLLKEIAIMKGAPASKEDITGCA
                     AAGFAEPFDTFRRIEERVTEETETIASDREREREEREREARLVREEVENLKKNINKGG
                     GKK"
     gene            complement(1341..2339)
                     /locus_tag="VFJ67_09680"
     CDS             complement(1341..2339)
                     /locus_tag="VFJ67_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7290694.1"
                     /translation="MNCERCSELLIEYMDGGLGPEERAAVAAHLSGCEECEREFEEYA
                     EIRRVVTEESPLPAPSADVMARLSKAARDSVTREKRPFWKRWPYSPVLIPVLSSAIAI
                     MVWFYYGHGGVDVMDTASRDVLASKMKEPANGYETRGQAMPESRREYPVVAESSPALG
                     EGGASDEGIAAAPAAPPEEETSSLMDQSPVEQMGSTRAKKALPGSETEKSEQARILSE
                     STSDDVGKDYSGLLRLALRQQTEGDCDASIRTNEILLQSSPEPPGDVQAQSYRSLAEC
                     YEKQGKLDLAVMNYTQLGRVSPKESGFVNSRIMRIRDGSQQKLSVVTPMPTPTPVN"
     gene            complement(2349..2984)
                     /locus_tag="VFJ67_09685"
     CDS             complement(2349..2984)
                     /locus_tag="VFJ67_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7290695.1"
                     /translation="MFGINSKSGLTDEEAMRKFQKDDEGSFDLLLARHGAGVLRFIIR
                     MTGAEKPQAEDLLQEIFMKVIERRKRYDPDQKFSTWLYSIARNHCIDFLRTESYRRHS
                     SLDAPLSMEEEGGAVVIDFIRSDEKDQEERAFDLEVKELIEIGVRGLKEEFREVFLLR
                     EMEGLSLEEIAVVTESPLGTVKSRLRYAYKGLRKVFTETGYFEERQKAKGV"
     gene            3243..>3506
                     /locus_tag="VFJ67_09690"
     CDS             3243..>3506
                     /locus_tag="VFJ67_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290696.1"
                     /translation="MTTKPKDLIYSVAEMPPIGRLIPLGFQFTVYIAVTLVYIVLISS
                     YGDVPEKVTANAISMAMIAIGIATILQSLWKGPIGSGYFASPVY"
BASE COUNT          697 a         1080 c          829 g          900 t
ORIGIN      
        1 catgctgtct tcctcctgct tgtgaatagt cggcagcgcc gatatgtact tccctccggc
       61 gagtgccgcc agtataagaa taaaaattcc gattatgaaa taactgttcg acatcttcat
      121 ttcttaccgc ctcccttatt gatgttcttt ttcaggttct cgacctcttc ccttacgagc
      181 cgggcttcgc gttcacgctc ctctctctcc ctctcgcgat cagaggctat agtctccgtc
      241 tcttcggtga ccctctcctc gatcctcctg aacgtatcga agggctcggc gaatcccgcg
      301 gcggcgcatc cagtgatgtc ctccttcgat gcgggtgcgc ccttcatgat cgcgatctct
      361 ttgaggagga gcgtcctctt ggtcctggcc gtccgcaccc tctcgtcgag ctctgtatag
      421 cgcgcctcga aatcgccgag cttcgctctg tcgcctgact gcgcgagcgc gagcttcccg
      481 accttctcct caagaagcct tttctttctc acggcctcga cggcgttctc ttccatgtct
      541 cgcctcacgt agtcttcggc acgctcgcgc cagtacttcg cctgctcgcc caacctccgt
      601 gacagcttct cctgctcgtc tatcctgaac ctgagcgcgc ctatcttgac cctcgccctc
      661 tcgagaccct cttccatctc ctcgacagcc tggttgagca tcttctcggg gtcttccgcc
      721 ctcgacaccc agtcgttaag ctgcgatttc actatcagtc cgacccgttt cttaagtccc
      781 atgacttctc tccttactgc ggatttaggg attattacga acgggcggga ggaaagtttt
      841 tgaagggtaa gagaaaagat tagggagctc ttatttgtca ttcccgaatg ttcttaatcg
      901 ggaatctcgc ctttgtattg tcccgacatt acagaatttg cggcgcagaa tctgcgccca
      961 taatcgattc cacccatctc ccctgcacga accggcaaag gagccaaacc cgttcgccct
     1021 gagcttgtcg aagggttgaa cgggtatcac tcctgaataa ctgagtgtgg aattatacta
     1081 ttcagcgtta acttcggatt tttaaccaat tccgttctac cttttcataa aatacactac
     1141 gtgtgtgatt tttgcttctt tcccggctac ggctcgtgcc ttcgccgaga cgagtcatca
     1201 aggaaaagaa gaaaaatatt tgtcattatt tctgtttttt gatacttgcg aaatattctc
     1261 aagaacaaaa cagatcgcca cgaccagaag acggtctcgc gaagacaata ttagttaaga
     1321 acggaggaga aagaccgtat ctaattcact ggcgtgggcg taggcatggg ggtgacaacg
     1381 ctcagcttct gctgtgaccc gtcccttatc ctcatgatcc tgctgttgac gaatccgctc
     1441 tccttgggcg agacgcgccc gagctgtgtg taattcatca cggcgaggtc gagcttcccc
     1501 tgcttctcgt aacactccgc gagcgagcgg taggactgcg cctggacgtc tcccggaggc
     1561 tcgggagacg actggagcag gatctcgtta gtccttatgg aggcgtcgca gtccccttcg
     1621 gtctgctgcc tgagcgcaag gcgcaggagt ccggaataat ctttgcccac atcgtccgaa
     1681 gtcgactccg agagaatccg ggcctgctct gatttctcag tttcagaacc ggggagagct
     1741 ttcttcgctc tggtcgaacc catttgctct acgggcgact ggtccatgag agaactcgtt
     1801 tcctcttcgg gcggcgcagc aggagcggcc gcaatcccct cgtccgaagc cccgccttcg
     1861 ccgagcgcgg gagaagactc cgccacgacg ggatattccc tcctcgattc aggcatggcc
     1921 tgacccctcg tctcatagcc attcgccggc tctttcatct tgctcgcgag cacgtcgcgg
     1981 gacgcggtat ccatgacgtc cacgccgccg tggccgtaat agaaccagac cattatagcg
     2041 atcgccgacg agaggacggg aataagaacc ggggagtaag gccacctctt ccagaacggg
     2101 cgcttctcgc gggtgacgct gtccctcgcg gctttggaga gccttgccat tacatccgcc
     2161 gatggcgcgg ggagcggaga ttcctccgtt acgacgcgcc tgatctcggc gtactcctcg
     2221 aactcgcgct cgcattcctc gcacccgctc aaatgcgcgg ccaccgcggc cctctcttcc
     2281 gggccgagcc cgccgtccat gtactctatc aaaagctcac tgcatctttc gcaattcata
     2341 ttttccggtc acaccccttt cgctttctgt ctttcctcga aataccccgt ctccgtgaaa
     2401 actttcctta atcctttgta ggcatatctg agcctgctct tcaccgtgcc gagcggagac
     2461 tccgtcacca ctgctatctc ctctaacgac agcccctcca tttctctcag caagaatact
     2521 tccctgaact cctctttcaa tcccctcacg cctatctcga tcagctcttt cacttcgagg
     2581 tcgaacgccc tctcttcctg atccttctca tcgctcctta taaaatcgat gacgaccgcg
     2641 ccgccctcct cttccatgga caggggagcg tcgagtgagc tgtgcctcct gtagctctcg
     2701 gtcctgagaa aatctatgca gtgattccgg gctatgctgt atagccaggt agagaatttc
     2761 tggtccgggt cgtacctttt tctccgctct ataaccttca tgaagatttc ctggagcagg
     2821 tcttccgcct gcggcttctc ggcgcccgtc atccttatta tgaacctgag cacaccggcc
     2881 ccgtgccggg cgaggaggag gtcgaagctc ccctcgtcgt ccttctgaaa tttccgcata
     2941 gcctcttcgt ccgtcagtcc gctcttcgag tttattccaa acaccggtta cccgtctcag
     3001 ctcacatgca agatacgaat attatacgat ggaaaagggg gaaagtttta atacgggaat
     3061 tgcacatgct tacatatgca gtttgttcca tcggcaaaca cctcgtgctg aaccctgtcg
     3121 cggatacaaa aatttatact gaaatcaggg tgaaaccgta taagaactca aattgtgagt
     3181 gttgaattac tactctcctt aaagtagatt cgtgtatctc ctttccgcgt gagggaccga
     3241 acatgacgac caaacccaag gacctcatat actcggtcgc cgagatgccc ccaatcggga
     3301 ggctgattcc gctgggattc cagttcacgg tctacatcgc ggtcacgctt gtctatatag
     3361 tgctcatttc gagctacggt gacgtgcccg aaaaggtcac ggcgaacgcc ataagcatgg
     3421 cgatgatagc cataggcatt gcgaccatac tccagtccct ttggaagggg cccatagggt
     3481 caggctattt cgcatccccc gtttac
//
LOCUS       DASSJH010000440         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1403145, whole genome shotgun sequence.
ACCESSION   DASSJH010000440 DASSJH010000000
VERSION     DASSJH010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403145"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFJ67_09695"
     CDS             <1..247
                     /locus_tag="VFJ67_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290697.1"
                     /translation="TDIEEKDILYRMDGVIRKKHTSGSGGFAMSRTCAARLSRKESTS
                     QASDDGFMKITMGVNVAACIQRRAGQGAGFRHYPDIQ"
     gene            247..2166
                     /locus_tag="VFJ67_09700"
     CDS             247..2166
                     /locus_tag="VFJ67_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7290698.1"
                     /translation="MDKDRKPNILAGSDENIFSELNRILSKRGIRVDRALTADEFLAH
                     LRENFYEAAVMDTTAIDFPIPEMIMRAKSVSQYTEIIMITEPGQEGGPDSDNPLDSLV
                     YGYVETPVNINYVATLALKAVEKQRLVKSSAGGKSSGRKNVIERATSGDESALSLLLS
                     TSVNSLNSAVMITDTKHNIIYINNAHTRTFGYTPDELMGKKSDALYPLDDPSGVSSKI
                     YEALIMVGWEGERLAMRKDGNAFPVYEKTSAVKDRDGRQIGVVSVTDDITARKRLQQA
                     LKESEERYRSFVETAKSAIIAVDGEGRIILFNPAAEEIFGYGKEEIMNKEFRCLFPER
                     YKDITSAELGKGTGSGFTNLKETTTEITGLTKNGEEFSAEVSFSTCRVEGRTILTAII
                     FDITERKNLQEQLLQSAKLAAVGELISGVTHEVNNPLAVVIGYSEMLLSEPELGGEAR
                     DAIRAIHAESERAKKVIQNMLSFARKHNPEKEVIQVNDVIEKTLGLTDYELRKNSVTV
                     VKDLDPDLPETVADPNQLQQVFLNLIINSQQAMSETEDTRQLTIRSRLKEPEGKDAGK
                     RNVIQVAFEDNGPGIPDASIKKIFDPFYTTKPKGKGTGLGLSVSFGIIKEHGGEIFVK
                     PNDGKGVTFFVELPA"
     gene            complement(2221..3501)
                     /gene="serS"
                     /locus_tag="VFJ67_09705"
     CDS             complement(2221..3501)
                     /gene="serS"
                     /locus_tag="VFJ67_09705"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET7290699.1"
                     /translation="MLDSKYLEENLEKVTARLKDRGFDFDSAAFRAIDQDRRKVIKLV
                     EELEHERNLGSKKVAELKRSGDKEAADKLQAELKVISDKIKDLGSERSKAEAGLREFL
                     LGVPNIPDESVPTGRDSSDNREVRKWGQPREFGFEPRDHVEIGKILDIIDLDRAAKIT
                     GARFALYKGPGARLERALINFMLDIHTKEHGYREVLPPFMANSDSFIGTGNLPKFAAD
                     LFKVEGTDYYLVPTAEVPVTNIHRDEILDGNDLPVKYVAYTPCFRSEAGSYGKDVRGI
                     IRQHQFNKVELVKFSAPESSYEELESLTANACRILEELELPYRVVVLCTGDMGFSSAK
                     TYDIEVWVPSEKTYREISSCSNFEDFQARRASIRYRQPGGGKPRLVHTLNGSGLAVGR
                     TVIAILENYQEPDGTVTIPKALVPYMGGVVRITP"
BASE COUNT          879 a          960 c          976 g          729 t
ORIGIN      
        1 gacggatatt gaggagaagg atatcctcta tcggatggat ggggtaatcc gtaaaaagca
       61 tacgtccggc agcggggggt ttgcgatgtc acgaacctgt gccgcccgcc tcagccggaa
      121 agagtcaaca tctcaagcat cggatgatgg attcatgaaa atcacaatgg gtgtaaacgt
      181 agctgcctgc attcagcgtc gtgcaggaca aggggcgggg ttccgccatt accccgacat
      241 acagtaatgg ataaggatcg caaacctaac atactcgcag gttcggacga gaatatcttc
      301 tcggaactga atcgtatact gagcaaacgc ggcatccggg tagacagggc tttgactgcg
      361 gatgaattcc tggctcatct gagagagaac ttttacgaag cggccgttat ggacactacg
      421 gccatcgact tcccgattcc cgaaatgatc atgcgagcta agtcggtttc gcagtatacg
      481 gaaataatca tgattacgga acccggacag gaggggggcc cggattccga caacccgctc
      541 gacagcctcg tttacggata cgtcgagact cccgtcaata taaattacgt cgccaccctc
      601 gcacttaagg ccgtcgagaa gcagagactg gtgaaatcat ccgcgggcgg gaagagctcg
      661 ggcaggaaga acgtcatcga aagagcgacg agcggcgatg aatcagcact cagccttctg
      721 ttatcgacgt ccgtgaacag cctgaactcg gctgtcatga tcaccgacac gaaacacaac
      781 atcatttata taaacaatgc ccacaccagg acgttcggat acacaccgga tgagcttatg
      841 gggaagaaga gcgacgcact ttacccgctc gacgaccctt ccggagtgag cagcaagata
      901 tacgaagccc tgataatggt cggatgggaa ggtgaaaggc tcgctatgag gaaggacggg
      961 aacgcattcc cggtgtacga aaagacctcc gctgtcaagg acagggacgg gcgtcagata
     1021 ggggtcgtgt cggttacgga cgatataacg gcgagaaaga ggctccagca ggcgcttaaa
     1081 gagtcggaag aaaggtacag gagcttcgtc gagacggcga aaagcgcgat catagccgtc
     1141 gacggagagg gcagaataat actcttcaat cccgccgccg aagaaatatt cgggtacggg
     1201 aaggaagaga taatgaataa agaattcagg tgtctcttcc ccgaacgcta caaggatata
     1261 acgagcgcgg agctgggtaa ggggacgggg agcgggttca caaaccttaa ggaaacgacg
     1321 acggagataa cgggcctcac aaaaaacggc gaggagtttt cggccgaggt gtccttctcc
     1381 acttgcaggg tcgagggaag aaccatcctt accgcaatta tattcgacat caccgaacga
     1441 aagaacctcc aggagcagct gctgcaatca gccaagctcg cggctgtggg cgagctcata
     1501 tcgggcgtta cccacgaggt aaacaacccg ctcgcggtgg tgatagggta ttcagagatg
     1561 ctgctatcgg aaccggagct aggcggcgaa gccagggacg cgatcagggc gatacacgct
     1621 gaatccgaga gggcgaagaa ggtaatacag aacatgctct ctttcgcccg gaagcataat
     1681 cccgagaaag aggtgataca ggtaaacgac gtaatcgaaa agacgctcgg gctcacggat
     1741 tacgagctca ggaagaattc ggtgactgtg gttaaggacc tcgaccccga tctcccggag
     1801 acggtagccg acccgaacca gctgcagcag gtattcctca acctgattat caactcacag
     1861 caggccatgt cggagactga agacacacga cagctcacta taaggagcag gctgaaagag
     1921 cccgagggga aagacgccgg gaagaggaac gtgatacagg tcgcattcga ggataacggc
     1981 cccggcatac ctgacgcgag catcaaaaaa atattcgacc ccttctatac gacgaaaccc
     2041 aagggcaagg gcacgggcct gggactctcg gtatcattcg gaatcatcaa ggagcatggc
     2101 ggagaaatct tcgtgaagcc gaacgatggc aaaggcgtga ctttcttcgt agagcttccg
     2161 gcatgaatta tttaagataa agcagctctc aaaggatccg aacacaaacc ggccggttca
     2221 ctagggagta attctcacca cgccgcccat gtagggcacg agcgcctttg gtattgttac
     2281 agtgccgtcg ggctcctggt agttctcgag tatcgcaatt acggtcctgc cgacggcgag
     2341 accggagccg tttaacgtat gcacgagcct cggtttgccg cctcccggct ggcggtacct
     2401 gatcgaagcc cttctcgcct gaaagtcctc gaagttactg caggacgaaa tctccctgta
     2461 cgttttctcg ctcgggaccc atacctctat gtcatacgtc ttcgccgagg agaaccccat
     2521 gtcgcccgtg cagagcacga ctacgcggta gggaagctca agctcctcta agatgcggca
     2581 ggcgttcgca gtgagcgact cgagctcctc gtacgagctc tctggcgccg agaacttcac
     2641 gagctctacc ttgttgaact gatgctgcct gattataccc ctcacgtcct tgccgtaaga
     2701 tcccgcctcg ctcctgaaac agggcgtata ggccacgtat tttaccggca ggtcgttccc
     2761 gtctagtatc tcgtccctgt gaatgttggt tacagggacc tcggccgtcg gcacgaggta
     2821 atagtcagtc ccttccacct tgaacagatc ggccgcgaac ttgggcaggt tgcccgtgcc
     2881 gataaagcta tcgctgttcg ccatgaaggg cgggagcact tccctgtatc cgtgctcttt
     2941 tgtatgtata tcgagcatga aattgatcag cgccctttcg agtctcgctc cgggaccttt
     3001 atagagcgcg aacctcgcgc ccgtgatctt cgcggccctg tcgaggtcta ttatgtcgag
     3061 aatcttcccg atctcgacgt gatccctggg ctcgaaaccg aactccctgg gctgacccca
     3121 cttcctgact tccctgttat cggaggagtc cctgcctgtg gggacggact cgtccggtat
     3181 attaggaacg cccagaagga attccctgag tccggcctca gctttcgacc tttccgagcc
     3241 gagatcctta atcttgtccg aaattacctt gagctcagcc tggagtttgt ccgcggcttc
     3301 cttatccccc gaccgcttca gctcggccac cttcttcgat ccgaggttcc tctcgtgctc
     3361 aagctcctct acgagcttta tcaccttccg cctatcctga tcgatagccc tgaacgcggc
     3421 ggaatcgaaa tcgaacccgc ggtccttcag ccgggccgta actttctcga ggttttcttc
     3481 aagatatttg gaatcgagca ttatcactta gtcctgtgct tcgtatttgt taatgaacct
     3541 tccc
//
LOCUS       DASSJH010000441         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1403210, whole genome shotgun sequence.
ACCESSION   DASSJH010000441 DASSJH010000000
VERSION     DASSJH010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403210"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFJ67_09710"
     CDS             <1..382
                     /locus_tag="VFJ67_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7290700.1"
                     /translation="IEMGIKDPDGEGTIASFNTGTAELAISTSGGYERVIDPGGKAYH
                     HLLGPATGKPGSDFLSVTIILDGDSALADAYATALFVMGKENALSFLKKHPEVGVFAV
                     LPGGGIYCSDRMKKLVRGLRTRGG"
     gene            592..1800
                     /locus_tag="VFJ67_09715"
     CDS             592..1800
                     /locus_tag="VFJ67_09715"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET7290701.1"
                     /translation="MPISSIEEALSDIKRGKMVILVDDKDRENEGDLVVAAEYATPET
                     INFMAKNGRGLICLALTKENADRIGLQPIKPEYNPVPQYTAFTISIDASHGITTGISA
                     YDRASTIRFAISDESKAEDFIRPGHVFPLICRKGGVLVRAGHTEGSVDIARLAGLKPA
                     AVICEIMNDDGDMARLPDLEKFAETHDIKIASIADLISYRLRAESLVRRAASANIPTE
                     FGEFQIIVYESDIDPQHHVAFVKGDINPDKDVLVRVHSECLTSDVFGSLRCDCGSQIR
                     QAMKIINDEGTGVILYMRQEGRGIGLINKIKAYALQDDGFDTVEANEVLGFKPDLRDY
                     GIGAQILLDLGVRNMKLLTNNPKKIKGLEGFGLQIVERVPIEIPPNGRNSSYLKVKKD
                     KLGHLLSMVD"
     gene            1814..>2024
                     /locus_tag="VFJ67_09720"
     CDS             1814..>2024
                     /locus_tag="VFJ67_09720"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET7290702.1"
                     /translation="MPKKHEGNLDAKGLKFAVVVSRFNDFITERLVSGALDVLYRHNA
                     SDDDIDLIRVPGSYELLYAVKKAAEK"
BASE COUNT          523 a          513 c          576 g          412 t
ORIGIN      
        1 tatagagatg ggtataaagg acccggacgg agaggggact attgcgtctt ttaataccgg
       61 caccgcggag ctcgccatat cgacgagcgg gggttacgag agggtcatag accccggagg
      121 gaaggcctat catcatctgc tcggtcccgc gacagggaag ccgggcagtg acttcctgag
      181 cgtgactata atcctcgacg gcgacagcgc cctcgcggac gcctacgcga cggctctttt
      241 cgtcatgggg aaagagaacg ccctctcgtt tctgaaaaag caccccgaag tcggggtttt
      301 cgctgttctt ccgggcgggg ggatttactg cagtgacagg atgaaaaaac ttgtccgggg
      361 gttaagaacg cgcggcggct gaagatgaag cctttccctg ttctcattat ttccacttca
      421 ctggcccata tcgtcctatg cgataagagg gtgttccgac ttgattttat cgaccccgta
      481 agctggaacg gcttatttga caaaacagga ctgtcgctta tattgtaagt cggataacct
      541 tagtaaaaga taagcgattt caattatttc agggttgagg tagaagaaaa catgcctatc
      601 agttccatcg aagaagctct ttccgatata aaaaggggta aaatggtgat ccttgtcgat
      661 gacaaagaca gggaaaacga gggcgacctc gtcgtcgccg ccgaatacgc gacgcccgag
      721 actataaatt tcatggccaa gaacgggagg ggacttatct gcctcgctct cacgaaggag
      781 aacgcagacc gcataggtct ccagcctata aagcccgaat ataacccggt ccctcaatat
      841 acggccttta ccatatcgat cgatgccagc cacggcataa ccaccggtat ctcggcttat
      901 gacagggcgt ccacgatacg tttcgcgata tcggacgaat cgaaagcgga ggatttcata
      961 aggcccggtc acgtattccc gctcatatgc aggaagggcg gggtgctcgt gagggcgggg
     1021 cacacggaag gctctgttga catcgcacgg ctcgcagggc tcaagcctgc cgctgtgatt
     1081 tgtgaaataa tgaacgatga cggggacatg gcaagacttc cggacctcga aaaattcgct
     1141 gagacacacg acatcaaaat cgcctcgatc gccgacctga taagctaccg cctgagggcg
     1201 gaaagcctcg ttagaagggc ggcatccgcc aacattccga ctgagttcgg ggagttccag
     1261 atcatagtct acgagagcga catagacccg cagcaccacg tcgcgttcgt caagggggat
     1321 attaaccccg ataaggacgt gctggtcagg gtgcattccg aatgcctcac gagtgatgtt
     1381 ttcggctccc tcaggtgcga ctgcggctcg cagataaggc aggccatgaa gattatcaac
     1441 gacgagggta cgggtgttat actttatatg agacaggagg gaaggggcat cggccttatc
     1501 aacaagataa aggcctacgc cctccaggac gacggattcg acacggtcga ggcaaacgag
     1561 gtcctcggat tcaagcccga tctcagggat tacggcatag gggcgcagat acttctcgat
     1621 ctgggcgtca ggaacatgaa gctcctgacg aacaacccga agaagataaa gggtctcgag
     1681 ggtttcggtc ttcagattgt ggagcgcgtc ccgatagaga taccgcccaa cggaaggaat
     1741 tccagttacc tgaaagtcaa aaaggacaag ctcggacacc tcctttccat ggtcgactga
     1801 agaggagata aaaatgccta aaaaacatga ggggaacctc gacgccaagg ggcttaagtt
     1861 tgcggtcgtg gtcagcaggt tcaacgattt cataaccgag aggctcgtga gcggcgctct
     1921 cgacgtcctc tataggcaca acgcctctga cgacgacatc gatcttatca gggtcccggg
     1981 ctcgtacgag ctcctctacg ctgtaaagaa ggcggccgag aaga
//
LOCUS       DASSJH010000442         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1616322, whole genome shotgun sequence.
ACCESSION   DASSJH010000442 DASSJH010000000
VERSION     DASSJH010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616322"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1258
                     /locus_tag="VFJ67_09725"
     CDS             101..1258
                     /locus_tag="VFJ67_09725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7290703.1"
                     /translation="MISGFTKPEFRNFSLATASNFFFYCNFSSFFLLPLYIKSLGGDD
                     AHIGYIMGSFGITSLGAIPFVTFLVDKYGRRRFMLLGYALMFLASLSYLYVDELSPLV
                     YALRLVQGFSFAFSFTAAGTFVADYVPSVSRAQGLGIFSAFTIAAYAVGPSLGEYVIE
                     LAGYHNFFLYSSFFSLISFVLAVFMRDGSFTPSRDPYGFGFFRLISSRKYALVLLSNL
                     VLAGGLGAVLNFIATFFRSKGLQAYYFFLTYTITVTAIRIFGGGMADKWGRKAVASPA
                     LLIVSLSLASMFFVDSALTAVLISFIFSFGYGSLYPTLSALMIDKAGEDERGKAMGAF
                     NASYSMGINFLAFPFGVIARDYGYEAMYFASGCLVFAGFILYTFFEREKVS"
     gene            1293..2114
                     /locus_tag="VFJ67_09730"
     CDS             1293..2114
                     /locus_tag="VFJ67_09730"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HET7290704.1"
                     /translation="MAIKRGDSVLVMSPDEKTYLVTVDEGRRMGTHLGELLLEKAIGM
                     EYGSVLQTTLLHPFILLEPTIEDRMMKVKRLTQIIYPKDAALIVLKTGLGSGMRVIEC
                     GSGSGALTIALANAVAPAGKVYAYDRNEKFMENARKNVQNAGFGAFAEFKLREAQEGF
                     DEEGVDAVVLDLPSPWDGIASASRALRGGGRIASLSPTYNQVERCVEVLAEEGFVFIE
                     TVEVLVRNIRVSTGKTRPMDRMVSHTGFLTFGRKALKKKASGISNTDSDDIELTI"
     gene            2139..>2659
                     /locus_tag="VFJ67_09735"
     CDS             2139..>2659
                     /locus_tag="VFJ67_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET7290705.1"
                     /translation="MKPGKDPVVVLVFIALAVAFYIYNTYVGKEVREGEYRVADVIDG
                     DTIAIEDGKGTLVRYIGIDTPEIARQDSPGDPFSEEAKEFNGKLVRGESVRLEYDNER
                     YDVYGRLLAYVFTDGVSVNEQLLRSGLATPLFIEPNVKYMEAFTSASDEAKKDKRGIW
                     GELGSMASPDGNG"
BASE COUNT          572 a          685 c          812 g          590 t
ORIGIN      
        1 tatacattgt caaagataga gtgtagcata tcatgcctcg atcctttaat atttacaagt
       61 caaatctaag cgtttagacg ggcgatttca agaagcgccg atgatatcag ggttcacgaa
      121 gccggaattc aggaatttct cattagctac ggcgtctaat ttcttcttct actgtaattt
      181 ttcgtccttt tttctactgc cattatatat caaaagtctc ggaggggacg acgcccacat
      241 aggctacatc atggggtcgt tcggcattac ctcgctgggc gcgatccctt tcgttacgtt
      301 cctggtcgat aaatacggga ggaggcggtt catgctgctg ggttacgcgc tcatgttcct
      361 cgcctcgctc tcgtatctct acgttgacga gctctctccc ctcgtctacg cgctccgtct
      421 cgtccagggc ttctcgttcg cgttctcctt cactgctgca ggcacgttcg tcgcggacta
      481 cgtcccgtct gtaagcaggg cgcagggtct cggcatattc agcgccttca cgatagccgc
      541 ctacgcggta ggcccgtcat tgggagagta cgtgatagag cttgccgggt accacaattt
      601 ctttctctat tcgtcgttct tcagcttgat atcgttcgtc ctcgcggtct tcatgcggga
      661 cgggagcttc actccgtcga gagacccgta cgggttcggc ttcttcaggc tgatatcgtc
      721 gcggaagtac gcgctcgtgc tgctctcgaa cttggtgttg gcggggggcc tgggtgcggt
      781 gctgaatttc atcgccacct ttttccgctc gaaggggctc caggcatatt acttctttct
      841 cacatacacg ataacagtga ccgcgataag gatattcgga gggggcatgg ccgacaaatg
      901 ggggaggaag gcggtcgctt ccccggcgct cctgatagtg tccctgtctc tcgcgtcgat
      961 gttcttcgtc gactcggccc tgacggctgt gcttatatcc tttatattca gcttcggata
     1021 cggctccctg taccccactc tcagcgccct gatgatagac aaggcggggg aggacgagag
     1081 gggaaaagcg atgggcgcat tcaacgcgag ctacagcatg gggataaatt tcctggcgtt
     1141 ccccttcgga gtgatcgcga gggactacgg gtatgaagcc atgtacttcg cctcaggctg
     1201 cctcgtattc gccgggttca tcctttacac gttcttcgag cgtgagaaag tttcttgaaa
     1261 agacgggcgt ttttaattag tctagcgtgg tgatggcgat aaagcgcgga gactccgtac
     1321 tcgtaatgtc ccctgacgag aaaacctacc tcgtgaccgt ggacgagggc aggcgcatgg
     1381 gcacgcacct cggcgagcta ttgctcgaaa aagcgatagg tatggagtac gggagcgtct
     1441 tgcaaactac cctccttcac ccgttcatcc ttctcgaacc cacgatcgag gacaggatga
     1501 tgaaggtcaa gaggctcacg cagataatct atccaaagga cgcggcgctc attgtcctga
     1561 agaccggact cgggtcgggc atgagggtga tcgagtgcgg ctcggggtcg ggcgcgctca
     1621 cgatagcgtt agctaacgcc gtagcgcccg ctgggaaggt ttacgcctac gacaggaacg
     1681 agaaattcat ggagaacgcg aggaagaacg tgcagaacgc gggcttcgga gcgttcgcag
     1741 aattcaagct gagggaagcg caggaaggct tcgacgagga gggtgtggac gcggtcgtgc
     1801 tcgacctgcc gtcgccatgg gacggaatcg cctctgcgtc ccgtgcgtta aggggcggcg
     1861 gaaggatagc gagcctgtcg ccgacataca accaggtgga gcggtgcgta gaggtgctcg
     1921 cggaggaagg gttcgtcttc atagagacag tggaagtgct cgtcaggaac atacgcgtga
     1981 gcaccgggaa gacaaggccc atggacagga tggttagcca cacggggttc ctcaccttcg
     2041 gcaggaaggc cctgaaaaag aaggcatccg ggatttcaaa tacagattcc gacgatatcg
     2101 aattaaccat atagcggcaa tttaaggaga aatgacggtt gaagcccgga aaagacccgg
     2161 tcgtcgtgct cgttttcatc gcgcttgccg tagcgttcta catttacaac acgtatgtgg
     2221 gcaaggaggt gcgggagggg gagtaccggg ttgcggacgt aatcgacggc gacacgatag
     2281 cgatagagga cggcaaggga acgctcgtca ggtacatagg gatagacacg ccggagattg
     2341 cgcgacagga ctcccctggc gatccgttct cggaggaagc gaaggagttc aacggcaagc
     2401 tcgtccgggg ggagagcgtc aggctcgaat acgacaatga acgatacgac gtctacggca
     2461 ggctcctcgc ttacgtgttc actgacggag tgtcggtgaa cgagcagctt ctccggagcg
     2521 ggctcgcgac gccgctgttc atcgagccta acgtgaaata catggaggcg ttcacgagtg
     2581 cttcggacga ggccaaaaag gataagaggg gtatctgggg cgagctcggc tcgatggcaa
     2641 gcccggacgg gaacggcag
//
LOCUS       DASSJH010000443         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1617297, whole genome shotgun sequence.
ACCESSION   DASSJH010000443 DASSJH010000000
VERSION     DASSJH010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617297"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..941
                     /locus_tag="VFJ67_09740"
     CDS             504..941
                     /locus_tag="VFJ67_09740"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7290706.1"
                     /translation="MRNFRIALLACVISLWVGSAYAGDTMSEGEKLERKMWTAIIAGD
                     NAAVESMIAPGFQSVHEDGARDRAGEIKLLEGLDPLKVQFSDFKVTEQGDTIIITYSV
                     SVAESIDGKNLTNPAPSPRQSVWIKTPSGWQWIAHANLNPITK"
     gene            1012..2379
                     /locus_tag="VFJ67_09745"
     CDS             1012..2379
                     /locus_tag="VFJ67_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7290707.1"
                     /translation="MSGDKRKIKTAFYADFAVSLVLLGALLAWKLLVRGEEPGFLVLI
                     LAALLLINAALCLYSAVEIGRGRGGAAAKVWLFLAVFVLFYAAADLVGGAIFIRLTLF
                     HNYPDKYVHHKMPPHFRYLMENSYGDYSVEMVTNNMGFRGRDIGEKKPGTYRIVMLGD
                     SFTMGEGVPDGSTFPLLVEEYLNAAGGRKYEVVNLGVEGYAPVLEYSLLKRMIGDLKP
                     DMVVLNFDMSDMLNEYAYRKDAVLDESGDVVSVDGFPEYERTRENTKDRALRWIRNHL
                     FITGILYETLNMRTLNRQAADPANINVNTGVERGSRMLLLHTLEAPQLKETAEMYSMI
                     EDSILRAKKLCDLHGCTFILSVYPWGHQVSDTEWVPGRNLYMTSGERISDRTVDELGT
                     FAGRNGIDFFNAFPSFRRYSGGERLYFMHDMHWTPAGQKLMAETLDGFIEKEIKKKPA
                     AHVSY"
BASE COUNT          579 a          616 c          771 g          527 t
ORIGIN      
        1 aataacggag cccagaaagt ggcacattgt ctgttaggat agagcgacga tttttcgttt
       61 gctcggaatc gtttccgtaa tgtcgggaga aaaacaagat tgccgcgctt cgtttcactc
      121 cgctcgcaat gacagataat ttgtctttcg ccgtgacaag cgaacgggtt ggagtgtcgt
      181 cgcagactga tgcatgtgta acccgtttat acttcgacga gctcagtacg aacggggtat
      241 gttggttaga tgagtgaaag gtgacaccct cacccgtacc ctgatttggt ttaagcgacg
      301 cagagccggc agtcaattca ctttttagct tctggtcaga ggcgaacgaa tgctcacttg
      361 ttcgcattcg ttccctcaag ggagagggga attcttggtt aggctgaaga gtggcggtag
      421 aatcgtaaag acacactacg tgtgcgccga agcgggtata taattgcgca tatatatcct
      481 atatcccgat tacggaggat tgaatgagaa atttcaggat agcgctgctg gcgtgcgtga
      541 tctccctctg ggtgggctcc gcatacgccg gagacacgat gagtgagggc gagaagctgg
      601 agaggaagat gtggacggcg ataatagcgg gggacaatgc cgccgtagag tctatgatcg
      661 cgcccgggtt ccagagcgtc cacgaggacg gggcgaggga cagggcgggg gagataaagc
      721 tcctcgaagg gctcgacccg ctcaaggtgc agttctcgga tttcaaggtg acggagcagg
      781 gggacacgat cataatcacg tacagcgtgt cggtcgcgga gtccatagac ggcaagaacc
      841 tcacgaaccc ggcgccttcg ccgaggcaga gcgtgtggat aaagaccccg tcgggatggc
      901 agtggatcgc gcacgcgaac ctgaacccga tcacgaagta ggcccgcccc ggccgggccc
      961 gttactcccg tagcgatccg ggatcgggtt tctgatataa tataatgagc cgtgtccggg
     1021 gataagagaa agattaaaac agctttttac gccgatttcg ctgtatcgct cgtactgctc
     1081 ggggcgctgc tcgcatggaa gctcctcgtc cgcggggagg agcccgggtt cctcgttctc
     1141 atactcgccg cgcttctgct gataaacgcg gcgctatgtc tctactcggc ggtcgagatc
     1201 gggaggggca ggggaggggc ggccgccaag gtctggctct tcctggccgt tttcgtattg
     1261 ttctacgccg cagccgacct cgtcggaggc gcgatcttca tccgtttgac tcttttccac
     1321 aactacccgg acaagtacgt ccatcacaag atgccccctc atttccggta cctgatggaa
     1381 aacagttacg gggattacag tgttgaaatg gtgacgaaca atatggggtt cagggggagg
     1441 gacatcgggg agaagaagcc gggcacgtac cgcattgtga tgctcgggga ttcgttcacg
     1501 atgggtgaag gggttccgga cgggagcacg ttccccctgc tggtagagga atatttgaac
     1561 gcggccgggg ggcggaagta cgaggtcgtc aacctcgggg tcgagggcta cgcccccgta
     1621 ctcgagtatt cactcctcaa gaggatgatc ggtgacctca agcccgacat ggtagtgctc
     1681 aatttcgaca tgtccgacat gctcaacgaa tacgcctaca gaaaggacgc cgttctcgac
     1741 gagtcgggcg acgtggtctc ggtcgatggc ttccccgaat acgagaggac gagggagaat
     1801 acgaaggacc gcgccctcag atggatacgc aaccacctct tcatcacggg aatcctgtac
     1861 gagacgctaa acatgcgcac gcttaacagg caggccgcgg acccggcgaa catcaacgtc
     1921 aataccggcg tcgagagagg gagccggatg ctgctgcttc acacgctcga agccccgcag
     1981 ctcaaggaga cggcggagat gtattcgatg atcgaggaca gcatactgag ggcgaagaag
     2041 ctctgcgact tgcacggttg tacgttcata ctgtccgtat atccgtgggg gcaccaggtg
     2101 agcgataccg aatgggtccc ggggagaaac ctgtatatga cgagcgggga gcggatatcc
     2161 gacaggacgg tcgatgagct cgggacattc gccggccgga acggtataga ctttttcaac
     2221 gcgttcccga gcttcaggcg gtactcgggc ggcgagcggc tatatttcat gcacgatatg
     2281 cactggactc ccgccgggca gaagctgatg gcggagaccc tcgacgggtt tatagaaaag
     2341 gaaataaaga aaaagccggc ggcgcatgtt tcgtattagg gaatatgtgg gaagcgggag
     2401 caattaagaa atacaaagaa cttttattat ccttcttttc cttgatgaaa ttacgaagaa
     2461 tggtgataag catttgtttt catccttctt ttc
//
LOCUS       DASSJH010000444         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1073371, whole genome shotgun sequence.
ACCESSION   DASSJH010000444 DASSJH010000000
VERSION     DASSJH010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073371"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..720)
                     /locus_tag="VFJ67_09750"
     CDS             complement(178..720)
                     /locus_tag="VFJ67_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7290708.1"
                     /translation="MDKIKVMIVEDEIIVAKHIEDSLQSLGYSVTGIVSSGEDAVELA
                     AGSHPDIALMDIMLEGETDGISAAEIIRKRYNIPVIFLTAYSNEKTIHRAKTAKPYGY
                     ILKPFEETDLFTSIEIALHKHRIERRLVEETENALAAIIGGTEVFLEDGTSKHNPETL
                     RRIESIRNAALIIKETIEEL"
     gene            complement(744..2750)
                     /locus_tag="VFJ67_09755"
     CDS             complement(744..2750)
                     /locus_tag="VFJ67_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7290709.1"
                     /translation="MRKTSGVQKDFPEGGSAKSYLSMVDSRAEDNGRADDSGLENVGK
                     SVSEEPAPSGSNPPKDRNSSLQKYSRYEKTVRIVTQKVHRSLDLNDVMDSAVDTMSKH
                     IETAQNVSIYLVEGKSAVLKSYRGYPDDYMHKLASIPYPKGFTWKTILESRPSHCPDV
                     DQDTVIGPLGRELGTKSYVSMPIRHAGRAIGCININSVRKNAFDEDELDLLEIVASSI
                     EAAINNARRAEALKKSREALRENVAKLKRKNRFERVISSITRSVHQSLEVPEVFENAV
                     EAIHSEVGEAQHVMIYMIESPVAHDSKPHAVLRAQRGHPESFIERVKSIPYPIGATWK
                     TIMDGRPRFITDIDTDPYVGQPARDFGSRSYISMPIKLVDDTVGCSHIHSSRKGAFSR
                     DHIKLLDIVARQIETAINNAKKTEALRESEEALRKTRDELEERVRERTSELQKSNTLL
                     IKEILERRYVENELKQSIAEKDVLLKEIHHRVKNNLQIISSLLNFQSRKLKDEKALAS
                     FVESNNRIRSIATLHEQLYRSKDLSRIDFGAHIRNMTNHLLRSYGVGGDCIRVNIDSE
                     QIYLDINTAIPLGLIVNELVSNAMKHGFEGMEKGEIKIEFRRSRDKYTLGVSNNGNRF
                     PNGLELGSCTTLGLELVSSLTKQLKGVITMTTGDITEFWLEFCA"
BASE COUNT          667 a          830 c          758 g          917 t
ORIGIN      
        1 aattctcttg gggcatgtct gtcggatacg tacacgcagg ggctcccgga ttttatcccg
       61 tggatgaatt agttggctgc cacctcataa cattccgatt cgttctcgta gaggtggcac
      121 aactgttcgt gctcgccgaa ggatttaaga aacgtaaata cggaattttc cataccgcta
      181 gagttcctct atcgtctcct tgataatgag cgccgcgttt cttatggact cgattcttct
      241 gagcgtctcc gggttatgct tcgacgtgcc gtcttcgaga aacacctccg tgccccctat
      301 tatcgcggcg agagcgttct cggtctcctc cacgagcctc ctttcgatcc tgtgcttgtg
      361 gagagcgatt tcgatcgacg taaacaggtc cgtctcctcg aaaggcttaa gtatgtagcc
      421 gtagggtttg gccgttttgg ccctgtggat cgttttctca ttggaatagg cggtgaggaa
      481 tataacgggg atgttgtacc gcttcctgat tatctcggcc gcgcttatac cgtcggtctc
      541 gccctcgagc atgatgtcca tgagcgctat atccggatgg gacccggcgg cgagctcgac
      601 cgcgtcctcg cccgacgata ctattccggt cacggaatac ccgagactct gaagcgaatc
      661 ttcgatatgc tttgcaacga ttatctcgtc ctctactatc attactttta tcttgtccat
      721 attccctatc ccttgaatat tttttatgcg cagaattcga gccagaactc ggtgatgtcg
      781 cctgtagtca tggtgataac gccctttaac tgcttcgtca gcgacgatac gagctcgagg
      841 cccagggtcg tgcagctgcc gagttcgaga ccgttcggga acctgttccc gttattgctc
      901 acgccgaggg tgtatttatc ccgggacctc cggaattcga tctttatctc gcctttctcc
      961 attccctcga atccgtgttt catggcgttc gatacgagct cattcactat cagcccgagc
     1021 gggatcgcgg tatttatgtc gagatatatc tgctcggaat cgatattgac cctgatacag
     1081 tcgcctccta ccccgtacga gcgcagcagg tggttggtca tattccttat gtgcgcgccg
     1141 aaatcgatcc tcgacaggtc cttcgatcgg tacagctgct cgtggagcgt tgcgatagac
     1201 cgtatgcggt tgttgctttc gacaaaggag gcgagcgcct tctcgtcctt gagcttccgc
     1261 gactggaaat tgagaaggct tgatatgatc tggaggttgt tcttcaccct gtgatggatt
     1321 tctttaagga gaacgtcttt ttcagcgatc gattgcttaa gctcattctc gacgtatctc
     1381 ctctcaagaa tctcttttat aaggagcgtg ttcgatttct ggagctctga cgtgcgttcc
     1441 ctcacgcgtt cttcgagctc gtctctcgtc ttgcggagag cctcttcaga ctccctcagc
     1501 gcctccgttt tctttgcatt gtttatggcc gtctcaatct gcctggcgac tatgtcgagc
     1561 aatttgatat ggtccctgct gaacgcgcct ttcctggagg aatgaatgtg actacaaccg
     1621 acggtgtcgt ccacgagctt gatcggcatc gagatgtagc ttcttgaacc gaaatctctc
     1681 gcaggctgtc cgacataggg gtccgtatcg atgtctgtta tgaaccgcgg cctgccgtcc
     1741 atgatggtct tccaggtggc tccgatgggg taggggatac tctttacccg ttcgataaaa
     1801 ctttccggat gcccgcgctg ggccctcaaa acggcgtgcg gctttgagtc atgcgcaacg
     1861 gggctttcta tcatataaat catgacatgc tgcgcttctc cgacttcgct gtgaatcgct
     1921 tcgactgcgt tctcgaacac ctccggtacc tcaagcgact gatgcacgct cctcgttatg
     1981 gagcttatga ctctttcaaa ccggttcttc ctcttcagct tggcgacatt ttccctgagg
     2041 gcttcacgcg actttttaag agcctcggcc ctcctggcgt tgtttatcgc cgcttcgatc
     2101 gatgaagcga cgatttcgag aaggtcgagc tcatcctcat cgaaagcgtt tttcctgaca
     2161 gaattgatgt tgatacaccc tatcgccctg cccgcgtggc ggatgggcat ggaaacataa
     2221 gacttggttc cgagctccct tccgagagga cctataactg tatcctggtc cacgtcaggg
     2281 cagtgtgaag gtctggattc cagaatcgtc ttccacgtaa aacccttcgg atacggtata
     2341 gaggcaagct tgtgcatata atcatcggga tatcccctgt aagacttcag aaccgcgctc
     2401 ttaccctcga ccaagtagat cgagacgttc tgcgcagttt cgatatgctt gctcatggta
     2461 tcgaccgcgc tgtccataac gtcgttgagg tcgagagacc tatggacttt ctgcgtgacg
     2521 attctgaccg tcttttcgta tctgctatat ttctggaggc tgctgttccg gtcttttgga
     2581 gggtttgagc cggacggagc gggctcttcc gatactgatt tgcctacgtt ttctaaacct
     2641 gaatcatccg cccggccgtt atcttcagcc ctggagtcaa ccatagacaa gtacgacttc
     2701 gcggagccgc cttccgggaa gtctttttga acacccgatg ttttcctcaa cgaattccca
     2761 gctcccccga gatttataag acctgcaaat tagtcgggtt agtcctcatt taaatagtca
     2821 atgattttca ttcggactat cggtatcacc atctcaaccg tttaagccga cgatgacgag
     2881 tttattttat agtctatcac aacataaact ttcaattcca gttctttcaa attcaattaa
     2941 ttaaccgttc cgggttcgag aatgcgtatg aggcccgaat cattatattt cataacggaa
     3001 aaatacatac tcaccctatt taaaatacgg taatcaccct attgaaatga aggaaagcat
     3061 acgatttaat gatataatca aaaaatattt ataacattaa ttattatcat gcagcgaggg
     3121 ggatatcatg ggtatgttat tcgcacataa tcgtgccgat acggaagatc gt
//
LOCUS       DASSJH010000445         4782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_106162, whole genome shotgun sequence.
ACCESSION   DASSJH010000445 DASSJH010000000
VERSION     DASSJH010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106162"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1092
                     /locus_tag="VFJ67_09760"
     CDS             79..1092
                     /locus_tag="VFJ67_09760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7290710.1"
                     /translation="MLDGITNRIINGIFNQTMLKKEKNIRSFTNQTPFDTVLAVLDSL
                     PEHIGVLDTSGVLVAANRSWIRHRPKNRGGAAERPMPGTNYLAMLRKHAEENRRSLRL
                     WKNILSVIDGKKDRFTMEYASHQDDGRKWYLLSVTPMRSGQSVTGAVISHTDITQRKL
                     AELESRRFSVTDPMTGILNRKAGLDYVHKQMKFCDRHGCSFTVCYIDLDNLKLVNDSF
                     GHKEGDRIIITFVNLLRRALRESDVMCRLGGDEILLILQETTLEGSNSLLKRILESVD
                     ARNTRSAKPYRIEFSYGLAEYTLGSKLTAEELVDRADRNMYAMKTSRKAGKDGNYGAR
                     SQQ"
     gene            1277..4747
                     /locus_tag="VFJ67_09765"
     CDS             1277..4747
                     /locus_tag="VFJ67_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7290711.1"
                     /translation="MTGGIHTRSDSITPSHTPLPACKRKRHEGATAIRAEGYPGRKFF
                     DLISERAETVDGISRILDCVYCEIYLFDSLTLLFRLVNKSARENLGYSADELGCMTPL
                     DLMRDFTPEHLANLLHKLGTGEEEEIGYSTFHYRKDGSCYPAEVKLREWTFKGAPAYL
                     ATALIATDLSITEERLRNTLDDLAKLNRYETVMNIVTQAVHKTIDLHMVMENAVEALS
                     RNMDAVDNICIYMREGWEAVMQAHRGYPDWFVERVKTIPFPKGFTWKTLIDGKMIYVA
                     DTEKDYVIGQAGRELGTKSYLSIPLKSEDRTVGAIVINADTKYAFGPDELRVLEIVSR
                     QIEIAINNAKFAETLVASEKALKEKIKQISKKEKYEKIINTVSRSVHSTLNLKDVMEN
                     AVKTLGENIEHADMVHIHLVEGDFAVQQSSRGVPDSLLSVVKRIPRPRGLTWKTILGG
                     KTLYVPDTDSDTAIGPAGKEAGIKSYVAMPIKINGATIGCIGINSFDKNVFSEDELSL
                     LEIIRGQMETAILNAKHVEALIMSEERYQTLTEILPIGIFRSDTEGRGVYVNNKLCEI
                     TGITKDDAYSDGWVDRLHPDDRDYVFSRWKEAVRTQSEFKEEYRFINRGGKTVWVIAE
                     AREEMDIQGEFKGYVGTLTDITDRKLSEEKILFQASLLDQVRNSVISTDLDEKIIYWN
                     KYAEEIYQWKEEEVRGKHIIDVVVPEGSREAVENMVRSMADTGHWEGDVAGKRKDGTT
                     FPVYLVLSAVRDERGKVIGRVGVGADITEKKMLEGRLLRAQRLESIGTLAGGIAHDLN
                     NILQPIMLSIQLLKPGVKGEKYERILNVVESSAERGADLIRQVLSFARGVESSKQTLN
                     TKYLISDALKIMRETFPKSIEIKTYTDGEIRNITGDYTQLHQVILNICVNARDAMQAG
                     GELSISTRNMSTGEDGTEKYYYIEPGEYVEITISDTGSGIPPDVMDKIFDPFFTTKEQ
                     DKGTGLGLSTAYGIIKEHGGYIHAESELGRGTRFIIYLPAIPMKGQTESGAYEDIKVA
                     AGTGETVLVVDDEPAVLDITRAMLEEFGYKVLTANNGHEALDVISDGKKRIHAAIVDM
                     MMPVIGGKTIIRSLKKKHPSLKIISVSGYQKEHELITMDENPADAFLNKPYNAETLLR
                     TLDTVLHGEG"
BASE COUNT         1311 a         1224 c         1438 g          809 t
ORIGIN      
        1 atccgccgag cccgcggggc tcgtagatat atattaaacc gggatctatc attaaataaa
       61 tcgtcccggt taaggaccat gctcgacggt ataacgaaca ggataataaa cggtattttt
      121 aaccagacca tgctgaagaa agagaagaat atacggtcat ttacaaacca gacgccgttc
      181 gacacggtgc ttgcggtgct ggactccctc cccgagcaca tcggggtgct cgacacgtcg
      241 ggagtgctcg tggcagccaa ccgctcgtgg ataaggcaca ggcccaaaaa ccggggcgga
      301 gcggccgaaa gaccgatgcc ggggaccaat tatctggcta tgctcaggaa gcacgcggaa
      361 gagaacagga ggtcgctgcg cctgtggaag aacatactgt ccgtgatcga tggcaagaag
      421 gacaggttca ccatggagta cgcttcccac caggacgacg gcagaaaatg gtacctccta
      481 tccgtaaccc cgatgagaag cgggcagtcg gtgacaggtg cggtcatatc gcatacagac
      541 atcacccagc gcaagctcgc ggagctcgag tcgaggaggt tctcggtaac ggacccaatg
      601 acggggatac tcaacaggaa ggccggcctc gattacgtcc acaagcagat gaaattctgc
      661 gacaggcacg ggtgcagctt cacggtctgt tacatagacc tcgacaacct caagctcgtc
      721 aacgacagct tcggccataa ggagggggac aggataatca taacgttcgt caatcttctc
      781 aggcgggcgc tcagggaatc ggacgtcatg tgcaggctcg gcggggacga gatactcctc
      841 atactccagg agacgacgct cgaagggagc aactccttgc tcaagaggat tctggaatcg
      901 gtggacgcca ggaacacgcg gagcgcaaag ccataccgca tagagttcag ctacgggctt
      961 gccgagtaca cgctcgggag caagctcacg gccgaagagc tcgtggacag ggccgacagg
     1021 aacatgtacg ccatgaagac ctccaggaag gccgggaaag acggcaacta cggagcccgc
     1081 tctcaacagt agcagtctcc gtcctcagct cgcaaaagac aataattcag gccaaaagcc
     1141 ccgtcctcca tgcccgcgcc ttgcggccgc gtatcccgcg agcgtaaccc ctaataaaca
     1201 ctcataggca tatacaagta tcggggcccg tttttaggct ttcccgaact ctatcgccga
     1261 atcggatgta tattttgtga cgggaggcat ccacacccga agcgactcga taacaccctc
     1321 acacacaccg ctccccgcct gtaagcgtaa acgacacgaa ggagctaccg ctataagggc
     1381 tgaaggttat ccgggacgaa aattttttga tctcatctcc gagcgcgcgg aaaccgttga
     1441 cggcataagc cgcatactgg actgcgtata ctgcgaaata tacctgtttg acagcctgac
     1501 gctcctgttc cgtctggtaa ataaatccgc gagggaaaac ctcgggtatt cggcggacga
     1561 gctcgggtgc atgacgccgc tcgacctgat gagggacttc acgcccgagc atctcgcaaa
     1621 cctgcttcac aagctcggta cgggcgaaga agaggagatc gggtactcga cattccatta
     1681 cagaaaggac ggctcgtgct atccggccga ggtcaagctc agggagtgga cgttcaaagg
     1741 agccccggct tatctggcga cggcgctcat cgcgacagac ctgagcataa ccgaggagcg
     1801 ccttaggaat actctcgacg acctggccaa gctcaaccgc tacgagacgg tgatgaatat
     1861 cgtcacgcag gcggtgcata aaactatcga cctccacatg gtcatggaga acgccgtcga
     1921 ggccctcagc cggaatatgg atgcggtgga caacatatgc atatacatga gggagggctg
     1981 ggaagcggtt atgcaagccc acagggggta tccggactgg ttcgtcgaaa gggtgaagac
     2041 cataccattc cccaaagggt tcacgtggaa gacgctcata gacggaaaga tgatatacgt
     2101 agccgatacg gagaaggatt atgtaatcgg acaggcgggc agggagctcg gcacgaagag
     2161 ctatctgtcc ataccgttga agtcggagga ccggaccgta ggcgcgatag tgataaacgc
     2221 ggatacgaaa tacgcgttcg gcccggacga gctgcgggtg ctggagatag tgtcccgcca
     2281 gatagaaata gccatcaaca acgcgaaatt cgccgaaacc ctggtcgctt ccgaaaaagc
     2341 actcaaggaa aagataaaac agatatccaa gaaggaaaaa tacgaaaaga tcataaatac
     2401 cgttagccgg agcgtccaca gcacgctgaa cctgaaagat gtgatggaga acgcagtaaa
     2461 gacgctggga gagaatatcg agcacgcgga catggtgcat atccaccttg tggaggggga
     2521 tttcgctgtt cagcagtcga gccggggagt cccggactcg ctgttgagcg tagtaaaaag
     2581 gataccccgc cccagaggcc tgacgtggaa aaccatactc gggggtaaaa cgctctatgt
     2641 acccgacacg gattccgaca ctgcgatagg gcccgcgggg aaggaagccg gaataaagag
     2701 ctacgttgcc atgcccatca agataaacgg cgcgacgatc ggatgcatag gcattaattc
     2761 gttcgacaag aacgtcttct cggaagacga gctcagcctt ctcgagatca taagggggca
     2821 gatggagacg gcgatactca acgcgaagca cgtggaggcg ctgataatgt ccgaggagcg
     2881 gtaccagacg ctcacggaga tattgccgat aggcatcttc cgctcggata cggaaggcag
     2941 gggagtgtat gtgaacaaca agctctgcga gataacgggg atcacgaaag acgatgcgta
     3001 cagcgacggc tgggtggaca ggctccaccc tgacgaccgc gattacgtct tttcgagatg
     3061 gaaagaggcc gtgaggaccc agagtgagtt caaggaagag taccggttca tcaaccgcgg
     3121 ggggaagaca gtatgggtca tcgcggaagc ccgggaagag atggacatcc agggggagtt
     3181 caagggctat gtcgggacgc tcaccgacat cacggaccga aagctctcgg aagagaagat
     3241 actctttcag gcgtcgctcc tcgaccaggt aaggaactcc gttatatcga ccgacctcga
     3301 tgaaaaaatt atatactgga acaaatacgc cgaggagatc taccagtgga aggaagagga
     3361 agtccgcggg aagcatatta tcgatgtggt cgtcccggag ggatctaggg aagcggttga
     3421 gaatatggtc aggtcgatgg ccgataccgg ccactgggaa ggagacgtcg caggtaaaag
     3481 gaaggacggg accactttcc ccgtttatct cgtgctgtcg gccgtaaggg acgagagggg
     3541 gaaagtcatc gggcgcgtcg gagtcggcgc cgatattacg gagaaaaaga tgctggaagg
     3601 aaggctgctg agggcccaga ggctcgagag catcggcact ctcgccgggg gaatagccca
     3661 cgacctcaac aacatcctcc agccgataat gctgtccata cagctgctca agccgggggt
     3721 caaaggcgag aaatacgaga ggatactgaa cgtcgtggaa tcgagcgccg agaggggagc
     3781 cgacctaata agacaggtgc tgtcgttcgc gcggggcgtg gagagctcaa agcagacgct
     3841 caacactaag tacctgatat cggacgccct caaaataatg agggagacgt tcccgaaatc
     3901 gatagagata aagacctata ccgacggaga gataaggaac ataaccgggg actacacgca
     3961 gctccaccag gtgatactta atatatgcgt gaacgcgagg gacgccatgc aggcgggcgg
     4021 agagctctcg atctcgacga gaaacatgag caccggcgaa gacgggacgg agaaatacta
     4081 ctacatcgag cccggggaat acgtcgagat aaccatatcc gatacgggct cagggatacc
     4141 gcccgacgtc atggacaaga tattcgaccc gttcttcaca actaaggagc aagataaggg
     4201 gacgggactc gggctctcga ccgcgtacgg aataatcaag gagcacggag ggtatataca
     4261 cgcggaaagc gagctcggca ggggcaccag gttcatcata tacctgcccg ccatccccat
     4321 gaaaggccag acggaatctg gcgcatacga ggacataaaa gtagcggccg gaacggggga
     4381 gacggtgctc gtagtggacg acgagcccgc cgtgctcgat ataacgaggg ccatgctcga
     4441 ggagttcgga tacaaggtgc tgacggctaa caacgggcac gaggcgctcg acgtgatatc
     4501 ggacgggaag aaaaggatac acgcagcgat agtagatatg atgatgccgg tgatcggcgg
     4561 aaagaccatt atacgctcgc tcaagaagaa gcacccttcg ctcaagatca taagcgtgag
     4621 cggctaccag aaggagcacg agctcataac catggacgag aatccggcgg acgccttcct
     4681 gaacaagccg tataacgccg agacgctgct gcggacgctg gatacagtat tgcatgggga
     4741 agggtgagct gcggctttgg cgatcccgcc tcttttttca tt
//
LOCUS       DASSJH010000446         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1289461, whole genome shotgun sequence.
ACCESSION   DASSJH010000446 DASSJH010000000
VERSION     DASSJH010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289461"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..749)
                     /gene="cmk"
                     /locus_tag="VFJ67_09770"
     CDS             complement(93..749)
                     /gene="cmk"
                     /locus_tag="VFJ67_09770"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7290712.1"
                     /translation="MIITIDGPSGVGKSTVSKSVARKLGFTYLDTGAMYRAVALQVKR
                     QSVDPGDDKRLKSLLDNTEIRFVRNNNGDPVILLNNEDVTGLIRTPEVSRLSSDVATK
                     RAVRVKLVELQQEIGKDGNIVAEGRDMGTYVFPDAAFKFYLDASLDERARRRWKQLAE
                     AGGGGDIESVKNEIIKRDSQDMERSESPLHPAVNAVIIDTTDLTSDEVTDRIIKEIQG
                     "
     gene            complement(809..2101)
                     /gene="aroA"
                     /locus_tag="VFJ67_09775"
     CDS             complement(809..2101)
                     /gene="aroA"
                     /locus_tag="VFJ67_09775"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7290713.1"
                     /translation="MAGEKIKGNSGPLRGEITPPGDKSVSHRSLIIGSLASGTTEVNG
                     FLNCEDTISTASAMRKLGAVIDINGSDVKITGKGLSGLSEPDDVIDAGNSGTTTRLLA
                     GLLSAQGFFTAVTGDRYLRARPMKRVVDPLRLMGARITGREGGNKLPLAIEGGSLKGV
                     SYRLPVASAQVKSALLLAGMYAEGETEVIEPEPTRDHTERMLRHFGVKLQKKGNSIKI
                     SRQKELEARDISVPADLSSAAFFIVGALISPGSELLIRNVGINPLRTGVIDVLRRMGG
                     NIDITNEREVSGEPVGDILVRASELRGTVIEGETIPKAIDELPVIAAAACFAEGETVI
                     RDARELRVKETDRIKAMTAELFNLGARVTEFDDGMSIKGGAPLAGASCSSWGDHRIAM
                     AVAIAAKRASGETEIDGAECVSVSYPGFFDVLRGFDTD"
     gene            2207..3187
                     /locus_tag="VFJ67_09780"
     CDS             2207..3187
                     /locus_tag="VFJ67_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290714.1"
                     /translation="MIHRLKTRASRSAAAIVLITLYLLFTPSTGGAALCKHWSGGEKS
                     GALDRLQLDEASGIAVSQRFPGRLYHVNDSGSGPYFYVTGSDGGKTKAVRIEGFDAGG
                     SDFEDASLGRCFGGKSCFFIADTGDNREKRKFVRIIVIEEAQDFGPSAVPLKVLKLKY
                     PERPHNAEGVALHPNGDIYIFTKEEDLDNMKSYPARLFKIEREKWENAGDGELMLEYA
                     GELDLPRLSGSDSNFGSVITSFDIAPDGKKFIVLTYEDAYEFDIDLSASGLKPSAGLV
                     RDKDYRVIKLVALPQQESISYIEEGRGFIYDSEYHMFDVPIMKVECLDGK"
     gene            complement(3199..>3729)
                     /locus_tag="VFJ67_09785"
     CDS             complement(3199..>3729)
                     /locus_tag="VFJ67_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET7290715.1"
                     /translation="SGFNVAILYMVSTTILHYTLGILILNYGESPFEIFRLPLVYSAI
                     AGVLLSVSGWTLPTPVFRAFDLLGEASIPTMIFSLGYKLSEVPLTDVSRSFLAGGMRI
                     FFGVLLGIAAVSIFKLDGVAAKVVILQSAMPPAIFNFVLAEKYNQDSERVASIILAGT
                     LISVITTPVIIGFLLH"
BASE COUNT          806 a         1087 c          923 g          913 t
ORIGIN      
        1 ccatagacct tccttcttta tcgtatatac ctattaaaac taactgtctg atttcatttt
       61 gggaagtaga atcaggtagt gaaaaactct tactaacctt gaatttcttt gattatcctg
      121 tctgtaactt catcagacgt cagatctgta gtatctatta tgaccgcatt aacagcggga
      181 tgcaagggag attccgagcg ctccatgtcc tgcgagtccc tttttattat ctcgttcttc
      241 acgctttcga tatcgccccc tccgcccgcc tccgcgagct gcttccacct tctccttgcc
      301 ctctcatcga gggaagcgtc gaggtagaat ttgaatgctg catcggggaa aacgtaagtc
      361 cccatatccc tcccctcggc cacgatattg ccgtctttcc cgatttcctg ctgcagctcg
      421 acgagtttta ccctgacggc cctcttcgtc gctacgtcgg acgacaatct cgagacctcg
      481 ggcgtcctta tcaagcccgt gacgtcttca ttgttcagca ggataacggg atcgccgttg
      541 ttattcctga caaaccttat ttccgtatta tccaggagag atttaagcct tttatcgtca
      601 cccgggtcga ctgactgccg ctttacctga agcgcgaccg ccctgtacat agcacccgta
      661 tcgaggtacg tgaaacccag ttttcgggcg accgatttcg ataccgtgct tttcccgacc
      721 cccgaaggtc cgtctatcgt tattatcata tcggttttaa tatcggcgga gcgggctact
      781 ccagcctaac tcagtgtaat attgcaactt aatcagtatc aaaacccctg agaacgtcga
      841 agaatccggg gtaggatact gaaacgcact cggcgccgtc aatctcggtt tctccacttg
      901 ctctcttggc cgcgattgcc acggccatag cgatcctgtg gtcgccccag ctcgagcaac
      961 ttgcaccggc gagcggagcg ccgcccttta tcgacatgcc gtcatcaaat tccgtaaccc
     1021 gcgcgccgag attgaaaagc tcagcggtca tcgcctttat cctgtccgtc tcctttaccc
     1081 tcagctccct cgcgtcccgt atgaccgtct ccccctcggc gaagcacgcg gccgccgcta
     1141 ttacaggtaa ttcgtcgatc gccttcggta tcgtctcacc ttcaattaca gtaccgcgga
     1201 gctcgctcgc cctcaccagt atatccccga ccggctctcc tgaaacctca cgctcgttag
     1261 ttatatctat attcccgccc atccgcctga gcacatctat cacgcccgtt ctcagcgggt
     1321 ttattccaac gttccttatc aaaagctccg accccgggct gataagcgcc ccaactatga
     1381 agaacgcggc cgacgacagg tcggcgggca cggatatatc gcgtgcctct aattccttct
     1441 gtctcgatat ctttatgctg tttcccttct tttggagctt cactccgaag tggcggagca
     1501 tcctttccgt atggtccctc gtcggctcgg gctctatgac ctccgtttcg ccctccgcgt
     1561 acatccctgc caggaggagg gccgatttca cctgcgcgct cgccacgggg agcctgtacg
     1621 atacgccttt caggcttccg ccttcgattg ccaggggcag cttgtttcct ccctccctgc
     1681 ctgtgatcct tgcgcccata agcctgagag gatcgaccac cctcttcatg ggtctcgccc
     1741 tcaggtacct gtctccggtg accgccgtaa aaaagccctg ggcgctcagt aatcccgcga
     1801 gaagcctcgt cgtcgtcccc gaatttcccg cgtcgataac gtcgtcgggc tcgctcaatc
     1861 cggagagccc ttttccggta atctttacgt cgcttccgtt tatatctata acagcgccca
     1921 actttctcat cgcgctcgcc gtggagattg tgtcctcgca gttgaggaac ccgttcacct
     1981 cggtcgtccc ggaagcgagc gagcctatta tgagcgatct gtgcgaaacg gatttatccc
     2041 ctgggggagt gatctcgccc ctaagcggtc ccgaatttcc ttttattttt tctccagcca
     2101 tggctttatc cgaagcccgt catatctgtc cggcaaaaaa ttaattttgc ctgataaata
     2161 gcggaggtaa aattgtacaa caattattcg gacattggag tattccatga tacatagatt
     2221 aaagacccgg gcctcccgct cagccgcggc gatcgtccta ataacgctct acctgctgtt
     2281 taccccctct acgggcggcg cggctctttg caagcactgg agcgggggcg aaaagtcggg
     2341 cgctcttgac cgtctacagc tggatgaggc gagcggtatc gccgtctcgc aaaggtttcc
     2401 ggggaggctc taccatgtaa acgactcggg gagcggcccc tatttctacg tgaccggttc
     2461 tgacggaggc aagaccaaag ccgtcaggat cgaaggcttc gatgccgggg gatccgattt
     2521 cgaggacgcc tctctcggga gatgtttcgg cggaaagtca tgctttttta tcgctgacac
     2581 cggtgacaac agggaaaaga gaaagtttgt ccggattata gttatcgagg aagcccaaga
     2641 cttcggacct tccgcagtcc cgctcaaggt tttgaaattg aaataccccg aacgtcccca
     2701 taacgctgag ggtgttgctc tgcatccgaa cggcgatatc tatatattca cgaaagagga
     2761 ggatctggat aacatgaagt cctaccctgc caggctattc aaaatagaaa gggaaaaatg
     2821 ggagaacgcg ggggatggag agctcatgct cgaatacgcg ggcgagctcg acctgccgcg
     2881 tctcagcggt tccgactcta acttcggcag tgtgataacg tctttcgata tagcccccga
     2941 cggcaagaaa ttcatcgtgc ttacgtacga agacgcttac gagttcgata tcgatctctc
     3001 cgcgtcagga ttaaagccct cggccgggct agtcagagac aaagattaca gggtaatcaa
     3061 gctcgtcgct ttacctcagc aggaaagtat ttcctatata gaagagggta ggggcttcat
     3121 atacgattcg gagtatcaca tgttcgacgt ccctatcatg aaggtcgaat gtctcgacgg
     3181 gaagtagccg gcagccggtt agtgtaaaag aaaccctata atgaccggcg tggtgattac
     3241 cgagatgagc gtgcccgcga gtataatgga cgcgaccctc tccgagtcct gattgtattt
     3301 ttcagccagt acgaaattga atatggcggg cggcatggcg gactggagaa ttactacttt
     3361 cgccgcgacg ccgtccaact tgaatatcga gacggccgcg attcccagga ggacgccgaa
     3421 gaatatcctc atcccgcccg cgaggaatga cctgctcacg tccgtgagcg ggacctccga
     3481 caatttatac cctagcgaaa atatcatcgt cggtatgctc gcctcgccca atagatcgaa
     3541 cgccctgaat acaggggtcg gcagcgtcca tccggagacg ctcagaagca ccccggctat
     3601 ggccgaatag acgagcggga gcctgaatat ctcgaacggg ctttcgccgt agttgagtat
     3661 gagaatgcca agcgtgtagt ggagtatggt cgtcgagacc atgtagagta tggccacgtt
     3721 gaagcccga
//
LOCUS       DASSJH010000447         5310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_640211, whole genome shotgun sequence.
ACCESSION   DASSJH010000447 DASSJH010000000
VERSION     DASSJH010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640211"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VFJ67_09790"
     CDS             complement(<1..1213)
                     /locus_tag="VFJ67_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7290716.1"
                     /translation="MIQLDNISLAYGSREIFKNLDWHIKDGRRIGLFGPNGVGKTTLL
                     NIVAGLAQPDSGAVIVPPAVTVGYLPQEVGDTMPGNTVLEEAMTAFREVLELERELES
                     VREELGLVSGDNAGERKKLLLRLDAIQNELNARGFHTLKYRTEKLLLGLGFEIRDLHK
                     SLGVFSGGWRMRVALAKLLLVNPDLLLLDEPTNHLDIESIDWLEKYLQGFGGTVILVS
                     HDQYFLDRMVDTIAELGMGRITEYAGNYSYYLAERKSRLAIHRAAYENQQRKIKETER
                     FIERFRYKNTKAKQVQSRIKALEKLERIPPPQSDDPAIHFKFPEPERSGKSVLELSVF
                     SKSYGEIRVFEDAGPLQISRGDKIALIGKNGAGKSTLARIVIGTEPFEGERREGYNVS
                     VTYFAQHLADSL"
     gene            complement(1250..1606)
                     /locus_tag="VFJ67_09795"
     CDS             complement(1250..1606)
                     /locus_tag="VFJ67_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290717.1"
                     /translation="MQIRRFLTLAVFLLAVSFAVTGCDAIKGLFNPFAGKWRSGLLEL
                     DFGSDRSFKFVIGSAISINLNGEYSYDDKTLVLKFDKGSEVTFSYEFSSDKKTLTLVP
                     ETDFDYIKTRLDFKKE"
     gene            complement(1643..2146)
                     /locus_tag="VFJ67_09800"
     CDS             complement(1643..2146)
                     /locus_tag="VFJ67_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290718.1"
                     /translation="MLKGFYFVLISTLAASLFYVFFGTVFGYRYPFFVISLFPLFLGA
                     HVTLSAVVYALSLRSDTKKLRAGMAGRARRTVFILVSALILSALFPASAVVGLYPVQM
                     AMFHLLILFYAGISLGALSLAGALYLLRNGIHLSLLNKYVFTFALIISLGISYMTVRM
                     FTLLPVG"
     gene            2297..2989
                     /locus_tag="VFJ67_09805"
     CDS             2297..2989
                     /locus_tag="VFJ67_09805"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7290719.1"
                     /translation="MNPVRPLIGISTDIKEGNFVIEDAYGRAVAKAGGVPLLIPSIPE
                     RRELLKEAVSRIDGLLLPGSRDMDPKFYNEDPHPKLRPMSIERTEAEFILLEESLKRG
                     IPVLGICGGMQLLNVFYGGSLYQDIASFLPDAIAHEKGAVHDILVEEDTLLSGILGAE
                     RLSVKSYHHQAVNAVGSGVKVSATSPDGIVEGIESEDSSYVLGIQWHPEREDSEYADR
                     IFRSFINECGGK"
     gene            complement(3019..3489)
                     /locus_tag="VFJ67_09810"
     CDS             complement(3019..3489)
                     /locus_tag="VFJ67_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290720.1"
                     /translation="MKDILRIILSSIKPDYRELRSVFFRALVVLFALCVSSAVALIGL
                     GFVVWSLYLYLATMLSPYLAALISGAGAVALALVVVVVVLYLTGYFGRSGKAGSKPRE
                     AHQAAAYDPMELVHRYPLESGLTAAIAGFIVGSSADSPRTLTEFLTLLKESGPK"
     gene            complement(3501..3794)
                     /locus_tag="VFJ67_09815"
     CDS             complement(3501..3794)
                     /locus_tag="VFJ67_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290721.1"
                     /translation="MGQEDLGQDLEALREDVTKLRADLSQLAKALLEKGKSETDSAKD
                     RVVEDLMAGLRSARVKGGDTVESVEHRIQEKPLMSLLIAFFVGLLLGKLFDRG"
     gene            complement(3929..4783)
                     /locus_tag="VFJ67_09820"
     CDS             complement(3929..4783)
                     /locus_tag="VFJ67_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET7290722.1"
                     /translation="MSSDKKNQANYLKPLISVLSALGIIVSLYLTYIHFTESQAAFCA
                     AGSECDTVRQSAFSTVMGIPVAAIGVAGYSVLLIASLLGMKKHTKWLILYILALAGFI
                     FSAYLTYVELFVIKAICMYCIFSAVLMTVIFISILPAKSGYHPRLSVSYIMVLSLAVA
                     ATVILGAALVQAEKFGSSGEPPSAGQQHANTFQTGLAMYMGQHGAVMYGSYKCPHCNQ
                     QKKLFGDAFKYIKYVECNPTGENANPSLCLAKGITNYPTWEIDGRFYEGAMPLEDLSS
                     ISGYDRRN"
     gene            complement(4867..>5310)
                     /gene="fadJ"
                     /locus_tag="VFJ67_09825"
     CDS             complement(4867..>5310)
                     /gene="fadJ"
                     /locus_tag="VFJ67_09825"
                     /EC_number="1.1.1.35"
                     /EC_number="4.2.1.17"
                     /EC_number="5.1.2.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="multifunctional enoyl-CoA
                     hydratase/3-hydroxyacyl-CoA
                     dehydrogenase/3-hydroxybutyryl-CoA epimerase; catalyzes
                     the formation of an hydroxyacyl-CoA by addition of water
                     on enoyl-CoA; exhibits 3-hydroxyacyl-CoA epimerase and
                     3-hydroxyacyl-CoA dehydrogenase activities: forms a
                     heterotetramer with FadI; similar to FadA2B2 complex;
                     involved in the anaerobic degradation of long and
                     medium-chain fatty acids in the presence of nitrate;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HET7290723.1"
                     /translation="MNPLSMLDRMIEDGRTGMRSGRGFYKYGRDEKKLDRSINKLLSL
                     EEEDSESVSQEYIQDRLVLAMVNEAMMCLQDEVIGNARDGDVGALLGLGFPIYRGGPF
                     RYVDSAGAGEVLKKLHNLSVRYGTRYTPPVILKNAAVGGNKFYEN"
BASE COUNT         1242 a         1441 c         1456 g         1171 t
ORIGIN      
        1 cgagggaatc cgccaggtgc tgggcgaagt aagtgacgga gacgttgtac ccctcccttc
       61 tctcgccttc gaacggctcc gtcccgatga cgatcctcgc gagcgtgctc ttgcccgctc
      121 cgttcttgcc tatgagcgcg atcttgtccc ctcttgagat ctggagcggc ccggcgtctt
      181 cgaacacgcg tatttcgccg tatgatttgg agaaaaccga taattcgagg accgactttc
      241 cagagcgttc gggctcgggg aacttgaaat gaatggcggg gtcgtcggac tggggcggag
      301 gtatcctctc gagcttctcg agtgctttaa tgcggctctg gacctgcttt gcctttgtgt
      361 tcttgtacct gaacctctcg atgaaccgct cggtttcttt aatcttcctc tgctgattct
      421 catacgccgc cctgtggatc gcgagcctcg acttcctctc cgcgaggtaa taggaataat
      481 tccccgcata ctcggtaatg cgccccatgc cgagctcggc aatcgtgtcg accatgcggt
      541 cgaggaaata ctggtcgtgc gatacgagaa tcaccgtgcc gccgaatccc tgaagatatt
      601 tctcgagcca gtctatgctc tcgatatcga ggtggttcgt gggctcgtcg agcagaagca
      661 agtccgggtt tacgagaagg agcttggcga gggccaccct cattctccat ccgcccgaga
      721 aaacgccaag agacttatgc aggtcccgga tttcgaaacc gagcccgagg aggagcttct
      781 cggtcctgta ctttaaagta tggaaacccc tcgcgtttag ctcgttctgg atggcgtcga
      841 gcctgaggag aagcttcttt ctctccccgg cgttatcgcc gctcacgagc ccgagctcct
      901 ccctcaccga ttctagctca cgctcgagct cgaggacctc gcggaaggcc gtcatcgcct
      961 cctcgaggac ggtgttgccc ggcatggtgt ctcccacctc ctgcggcagg taaccgaccg
     1021 tgacggccgg gggcacgatt acggccccgc tgtcgggctg cgcgagaccg gccacaatat
     1081 tgagaagcgt cgtcttcccg acgccgttgg gcccgaagag acctatcctc ctgccgtcct
     1141 ttatgtgcca gtcgaggttt ttaaaaatct cccttgaccc gtacgcgagg gaaatattat
     1201 cgagttgaat catgacgtgt tagcttcgga gtaatatcgg gaatattgtc tattcttttt
     1261 taaaatcgag cctcgtttta atataatcga aatcggtctc gggcacgagc gtcagggttt
     1321 ttttgtcgga gctgaactcg taggagaaag tcacctcgct ccccttatcg aacttcaaaa
     1381 cgagcgtctt gtcgtcgtag ctgtactctc cgttgaggtt aatggagatc gcgctcccga
     1441 tgacgaattt gaaagagcgg tcgctcccga aatcgagctc caggagtccc gacctccatt
     1501 tgcccgcgaa ggggttgaag agacccttta tcgcatcaca acccgttacc gcgaatgaaa
     1561 ccgcgagtaa gaacaccgca agtgtcagga accttcgaat ttgcatataa tctcccgctc
     1621 attattacga ctctcaataa gtttaaccga cgggcagcag cgtaaacatc cgtacggtca
     1681 tataagatat accaagagag attatcagtg caaatgtgaa tacatattta ttgaggaggg
     1741 acaagtggat tccgttcctc aggagataga gcgcccccgc aagactcagc gcaccgagag
     1801 atatcccggc gtagaagagt atgaggaggt ggaacatggc catctggacg gggtagaggc
     1861 ctacaacggc gctcgcgggg aagagggccg acagtatcag cgcggagacg agaatgaaaa
     1921 ccgttctcct ggcccttccg gccataccgg ccctcagctt cttcgtatca ctccggagtg
     1981 agagggcata gacaacggcg ctgagcgtca cgtgcgcccc gagaaagagg ggaaagagcg
     2041 agattacgaa aaaagggtac ctgtatccga ataccgtacc gaagaagacg taaaagaggg
     2101 acgccgccag agtggatatc aggacaaaat agaaaccttt cagcattatc gtatacgttc
     2161 cgtttacagg tgataagaat aatgcagaaa cgaatggcgc gttatgattt tttccgaggc
     2221 ccgtaattcc ggcttgcttt ttccgcgggg acaaatatct ttaggtcgga agacgaaacg
     2281 tcgcacaaag gaaagcatga accccgtaag gccccttatc ggcatctcga cagacatcaa
     2341 ggaaggtaat ttcgtcatcg aggacgcgta cggaagggcg gtggcgaagg ccgggggggt
     2401 cccgcttctc ataccctcca taccggaaag acgggagctc ctgaaagaag ccgtctcccg
     2461 catcgacggg ctccttctgc cggggtcaag agacatggac ccgaaattct ataacgagga
     2521 tccccatccc aagttgagac cgatgagcat cgaaagaacg gaggccgaat tcatattgct
     2581 cgaagaatca ctgaagagag ggataccagt gctcggcata tgcggcggca tgcagttact
     2641 taacgtcttt tacggcggct cattatacca ggacatcgcg tccttcctcc ccgatgcgat
     2701 tgcgcacgag aagggagcgg tgcacgatat actcgtagag gaagatacgc tcctgagcgg
     2761 catactcgga gcggagaggc tgtcggtcaa gagctatcac caccaggccg tgaacgcggt
     2821 agggagcggc gtcaaggtct cggcgacttc tccagacggg atcgtcgagg ggatagagtc
     2881 cgaggattct tcttacgtat taggcataca atggcatccg gagcgcgagg attccgaata
     2941 cgcggaccgg atattcagat cgttcataaa tgaatgcggc gggaaataaa aaaagaatcg
     3001 taaccggggc ggagagggtt acttcgggcc gctctctttg agaagcgtga ggaattcggt
     3061 aagcgtcctc ggagagtcgg ccgatgatcc cacaatgaat ccagcgatag cggctgtaag
     3121 gccggattcg agaggatacc tgtggacgag ttccatagga tcgtatgcgg cggcctggtg
     3181 cgcctcgcgg ggcttcgagc ccgccttccc gcttctgccg aaatagcccg tcaggtaaag
     3241 cactacaaca acgacgacga gagccagcgc cactgctccg gccccgctta tgagcgcggc
     3301 caagtaggga ctcagcattg tcgccaggta gaggtatagg gaccagacga cgaatccgag
     3361 cccgatcaaa gcgacagctg aagatacgca gagcgcaaac agcacgacca gggccctgaa
     3421 gaataccgac ctcagctccc tgtaatcggg cttgatcgag cttagtatta tccgcaatat
     3481 gtccttcaag ccgggagccc ctaccctctg tcgaagagct tgcccaaaag tagcccgacg
     3541 aagaaagcta taagcaggct cataaggggt ttttcctgga ttctatgctc aacggactcg
     3601 accgtatcgc cccccttgac gcgggccgac ctgagcccgg ccatcaggtc ttcgaccact
     3661 ctgtccttgg ccgaatcggt ctcgctcttc cctttttcga ggagcgcttt agcgagctga
     3721 gacagatccg ctctcagctt cgtaacgtct tcacgtaaag cctccagatc ctgtccaaga
     3781 tcttcctgac ccatttcaat caccttcccc gaatagattt attaataata ataacatctt
     3841 tgggcgcaaa tctaaactga tttttcctgc gcggggggcg ttcggatagc tgcgtgagga
     3901 gtttcccaat gagttgggca ggttaaatct agttccggcg gtcgtatccg gagatgctcg
     3961 aaagatcctc gagcggcatc gcgccctcgt agaacctgcc gtcgatctcc cacgtcggat
     4021 agttcgtgat gcccttggcc aggcagaggg agggattcgc gttttccccg gtcgggttgc
     4081 actcgacata tttgatatat ttgaatgcgt ccccgaaaag ttttttctgc tgattgcagt
     4141 gggggcactt gtaagaaccg tacatgacgg ccccgtgctg acccatgtac atggcaagac
     4201 cggtctgaaa agtgttggcg tgctgctgcc cggcggacgg gggctcgcct gatgagccga
     4261 acttttcagc ctgtaccagc gcagccccca gtattacggt tgcggcgacg gcgagactga
     4321 gcaccatgat atacgacacc gacaacctgg ggtgataccc ggacttggcc ggaaggatcg
     4381 agataaaaat aacagtcatc aggacggccg aaaaaataca gtacatgcat attgccttta
     4441 taacgaagag ctcaacgtaa gtgaggtacg cggagaaaat gaatccggcc agggccagta
     4501 tgtatagaat gagccacttc gtgtgtttct tcatgccgag gagagaggct atgagtaaga
     4561 ccgagtaacc cgcgacacct atggcggcga cggggatgcc catcaccgta gagaaggcgc
     4621 tctgcctcac cgtatcgcac tcggatccgg cagcgcagaa ggcggcctgg ctctcggtga
     4681 aatgtatgta agtaaggtag agtgagacta taattcccaa ggccgacagg accgagatca
     4741 gcggctttaa ataattagct tgatttttct tgtccgaact cataggagct aaattataac
     4801 taccggtcca gggatttcat accgtaaaat gagtaagcgc tgcagccgaa aatattaacc
     4861 tgtctatcag ttctcgtaaa atttgttacc gccgacggct gcgtttttca aaataaccgg
     4921 gggggtatag cgcgtgccgt acctgacgct caggttatgg agcttcttca aaacctcccc
     4981 tgctccggct gagtccacgt acctgaaagg gcctcccctg tagatcggaa agcccagtcc
     5041 gaggagcgcg ccgacgtcgc cgtccctggc atttcctata acctcgtcct gaagacacat
     5101 catggcctcg ttcaccatcg cgagcacgag acggtcctgt atgtattcct gcgaaacgct
     5161 ttccgaatcc tcttcctcga gggaaaggag cttgttaatc gacctgtcga gctttttctc
     5221 atcccgtccg tatttgtaga atccccttcc gctcctcata cccgtcctgc cgtcctctat
     5281 catccggtcg agcatggaga ggggattcat
//
LOCUS       DASSJH010000448         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_324240, whole genome shotgun sequence.
ACCESSION   DASSJH010000448 DASSJH010000000
VERSION     DASSJH010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324240"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..797
                     /gene="tmk"
                     /locus_tag="VFJ67_09830"
     CDS             180..797
                     /gene="tmk"
                     /locus_tag="VFJ67_09830"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7290724.1"
                     /translation="MLITFEGIEGSGKSTQANLLNEFLSGKGYKVTLTREPGWGHLGN
                     LIRTRILEERELVLAPMAELFLFCADRAQHVKDFIAPRLKNGEIVICDRFYDSTVVYQ
                     GYGRKLDMRLVNKAAKASALDVAPAITFLLNLPVREGLARLKERGSITKMDEEPIEFH
                     EMIRQGYMLIARREPERIKSVNAARDITTVHEEIRALVMERLTSQ"
     gene            844..1857
                     /gene="holB"
                     /locus_tag="VFJ67_09835"
     CDS             844..1857
                     /gene="holB"
                     /locus_tag="VFJ67_09835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET7290725.1"
                     /translation="MFEGIIGHDFQKDILKRAARDNMASHSYLFAGPDGVGKKLMALE
                     FAKLLNCPGSADSTDERCECSSCRKIERGIHPDVALVEFTGIKNIKVDQVREEIEEKL
                     YLRPFEGMFKVVIVDEAERMNHNAQNAFLKTLEEPPRDSVIILLSSRPETLLPTLRSR
                     CQTVVFNPLPEELVAGVLMERGGMSPEDAVLYSRISGGSIGKALGFDKELMEWRKDLL
                     TGLGSIRKNSASDISNLSELVSSGVSQNETERLELGFRFILLWIRDIVLAGIGSNGLT
                     NTDFTETINNEALKWDLDDLLEMERNVERAWYDIARANANTKLVLDNLFLKLSVYAGM
                     NRA"
     gene            1928..>2983
                     /gene="metG"
                     /locus_tag="VFJ67_09840"
     CDS             1928..>2983
                     /gene="metG"
                     /locus_tag="VFJ67_09840"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7290726.1"
                     /translation="MPKAFYVTTPIYYVNDVPHIGHAYTTIAADVIARYRRLKGDRVF
                     FLTGTDEHGQKIERTAEANDEKPIELANRVVKRFQNLSQALNITNDDFIRTTEKRHER
                     SVVEIFRRIEDAGDIYLDEYEGWYDVRNEAFITDTQYEEIMELPPEKRPIIERVKEES
                     YFFKLSKYQDRLLEHYRQFPEFVQPGYRMNEVARFVEGGLKDLSISRTTFNWGIPVPG
                     NPKHVIYVWFDALTNYITGAGFPDEPERFVGIWPADIHLVGKDILRFHAVFWPAFLMS
                     AKIPLPRTVFAHGWWTVEGEKMSKSLGNAIGVLDPPGDMYGKVMSIPDALLWSWYELL
                     TDVPADEIAARRRRIEAG"
BASE COUNT          734 a          737 c          879 g          633 t
ORIGIN      
        1 tcacggcatc cgagctcaag atgctcggcc gcgccctcga taagggggct gacgcggggc
       61 tgtatcaatt atgataatta tggtacgatt ctccggtgat tcgataattt atagccggtt
      121 aacgggcggt tagtctactc ctgaccccgg tttctcaccc tcgaggaaaa atccaaaacg
      181 tgcttataac attcgaaggc atagaaggca gcggcaaatc cacacaggcg aacctcctta
      241 acgaattcct gagcgggaag ggctacaagg ttacgctgac tagggagccg ggctggggac
      301 atcttgggaa cctcattcgc actagaatat tggaggagag ggagctcgtt cttgcgccga
      361 tggccgagct gtttcttttt tgcgccgaca gggcacagca cgtaaaggac tttatcgccc
      421 cgagactgaa aaacggagaa atagttatct gcgacaggtt ttacgattcg acggtcgttt
      481 atcagggcta cggcaggaag ctcgacatga ggctcgtcaa caaagcggcg aaagcctctg
      541 cgctcgacgt cgcgccggcg atcacttttc tcctcaacct cccggtcagg gagggcttgg
      601 cgaggctcaa ggagaggggg tcgatcacga aaatggacga ggagccgatc gagttccacg
      661 aaatgatcag gcagggatac atgctgatcg cgcggaggga gcccgaaagg ataaagagcg
      721 tgaacgcggc aagggatata acaaccgtgc atgaggaaat aagggcgctc gtgatggagc
      781 gattgacgtc ccaatgatgt cattggatta tgcgggattc gtccccgcat ggagcctgga
      841 gcaatgttcg aaggcataat cggtcacgat tttcagaagg atatactaaa gcgcgccgcg
      901 cgcgataaca tggcgtcgca ctcatactta tttgcgggcc cggacggtgt gggcaagaag
      961 ctcatggctc ttgaattcgc gaagctcctt aactgtccgg gatctgcgga ttccacggac
     1021 gaaaggtgtg agtgttcctc gtgcagaaag atagagaggg gcattcatcc ggacgtcgcg
     1081 ctcgtcgagt ttaccggcat caagaatatc aaagtcgatc aggtcaggga ggaaatcgaa
     1141 gagaagctgt atctcaggcc cttcgagggt atgttcaagg tggtgatcgt cgacgaggcg
     1201 gaaaggatga atcataacgc ccagaacgcg tttctgaaaa cactcgaaga accgccgcgc
     1261 gattccgtga taatactttt gtcgtcgcgt cccgagactc tccttccgac tctcagatcg
     1321 aggtgccaga cggttgtctt taaccccctg ccggaagaac tcgtcgccgg agtgctgatg
     1381 gagcgcggcg ggatgtcccc ggaggatgcg gtcctttact caaggatctc gggcgggagt
     1441 atcgggaagg ctctcgggtt cgacaaagag ctgatggagt ggagaaaaga tcttttgacc
     1501 ggtctcggaa gtataagaaa gaactcggct tccgatatct cgaacctctc cgaacttgtt
     1561 tcttcgggcg tttcccagaa cgagacggaa cggctcgagc tcggtttcag gtttattttg
     1621 ctctggattc gcgacatagt ccttgccggt attggttcga acggtttgac caataccgac
     1681 tttacggaaa cgatcaataa cgaggccctg aaatgggacc tcgacgattt actcgagatg
     1741 gagagaaatg ttgaacgagc ttggtatgat atagccaggg cgaatgccaa tacgaagctc
     1801 gtactcgata acctgttcct gaagctgtcc gtatatgccg gtatgaaccg agcataaggc
     1861 ggctttatat ttattgattg agaatttcct ccccggctcc ccgggttcgg aacggagaaa
     1921 gaaatagatg cctaaagcat tctacgtcac tacccctatt tattacgtga acgatgtgcc
     1981 tcacataggt cacgcctata cgactatagc tgccgatgtg attgcgagat acaggagact
     2041 caaaggggac agggtgttct ttctcaccgg tacggacgag cacggacaga agattgagag
     2101 gactgccgag gctaacgacg agaagccgat cgagctcgcg aaccgcgtgg tcaagcggtt
     2161 ccagaacctc tcccaggctc tcaacatcac gaacgacgac ttcataagga cgaccgagaa
     2221 gcggcatgaa aggtcggtcg tggagatttt caggaggata gaggacgcgg gcgatatata
     2281 tctcgacgag tacgagggat ggtacgacgt aaggaatgag gcgttcatta cggacaccca
     2341 gtacgaagag ataatggagc ttcctcccga gaagaggccc ataatcgaga gggtgaagga
     2401 agagagttat tttttcaagt tatcgaagta tcaggacagg ctcctcgaac actacaggca
     2461 attccccgag ttcgttcaac ccggctacag gatgaacgaa gtcgcgcgct tcgtggaagg
     2521 ggggcttaag gacctgagca taagccgaac cacctttaac tggggtatcc ccgtgccggg
     2581 taatccgaag cacgtcatat atgtctggtt cgacgcactg acaaattata ttacaggcgc
     2641 cggttttccg gacgagccgg agaggttcgt aggcatatgg ccggccgata tacacctcgt
     2701 gggcaaggac atactccgct tccacgcggt tttctggccg gcgttcctga tgtcggcgaa
     2761 gatacctctg cccaggaccg tattcgctca cggctggtgg acagtcgagg gggaaaagat
     2821 gtcgaagtcc ctcggcaacg ccatcggcgt gctcgatcct ccgggcgaca tgtacggcaa
     2881 ggtcatgtcc atcccggatg cccttttgtg gagctggtac gagctcctca ccgacgtgcc
     2941 cgccgacgag atcgcggcgc gccgccgccg gatcgaagcc ggt
//
LOCUS       DASSJH010000449        10905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_640362, whole genome shotgun sequence.
ACCESSION   DASSJH010000449 DASSJH010000000
VERSION     DASSJH010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640362"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..826)
                     /locus_tag="VFJ67_09845"
     CDS             complement(74..826)
                     /locus_tag="VFJ67_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET7290727.1"
                     /translation="MNEMHEKISAARDIIENCSSLTVLTGAGISAESGVPTFRGKDGL
                     WRNFRSEDLATAEAFWNNPRLVWEWYDWRRSAVKDAKPNPGHFALAELEGQMKKFTLI
                     TQNIDGLHQMAGSRNVIEMHGNIWQIRCTKCGDIQQSHKTPLEELPPRCVVCGEIGRP
                     NVVWFGEIIPMHVIDVSLKAIEECEVMLIIGTSGIVEPAASMGLVAKQTGKTVLEINL
                     EYTPNSPLFDMTILGKSGEILPLLYTSRKVFS"
     gene            complement(849..1985)
                     /locus_tag="VFJ67_09850"
     CDS             complement(849..1985)
                     /locus_tag="VFJ67_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7290728.1"
                     /translation="MMRSIKVGLSISLTGAYSVQGRESFEGISLWVSDVNKEGGIFVR
                     ELGRNLPIDLVYLDDRSSVDTCRANTEKLIAERKADILLGPYSSSLALASAEIAEGAG
                     VTLWNHGGSTDEMEGRGFTCLVNAITPTVRYAEGIIKLVRSRDPAARKIASFSAVDSG
                     FSRNVARGARLTAQENGFEMREFQFVSGSEDFSGLLDEALGFAPDLILGMGRAHDDIA
                     LARHVIERKVRVNAAAFVAASIKLFADTFGRSAEGFTSSSQWEEGIGVTPDAGPSPRE
                     FAGRFMSAYGKHPDYTAAQGYNIGLIVRKCIEDTGTLDDGALRKSARGKKLTTFYGQF
                     GTDGNGFQTAHEMVVVQWQGGKKVIVSPERFSEARFIYPMQPAY"
     gene            complement(2095..3288)
                     /locus_tag="VFJ67_09855"
     CDS             complement(2095..3288)
                     /locus_tag="VFJ67_09855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7290729.1"
                     /translation="MKLSQRAKKLQPSATLAITAKEKALKAQGVDVIGFGAGEPDFDS
                     PENVKEEAIKAIRAGFTKYTAVGGIDELKEAIINRMKLDYGLEYQKPEIIVSNGAKHT
                     LYNLTQAMFEEGDEVIVPAPYWVSYPEQITLAGADPVILNTLEEEGFKIDPAELKKLI
                     TPKTRALVLNYPSNPTGATYNEVELRLIVDVAMEAGLTIISDEIYDKIIFDGIKHTPV
                     ATLGEDVKKATILVNGVSKTYAMTGWRIGYAAGDKDVINAMGKLQGQSTSNPTSISQW
                     AALEAYRGPHDVIEERTREFEKRKNYIVERLNAIPGMKCFSPQGAFYAFPNVSGFYGK
                     RYNGKDVNSSMSFTEFLLDEAKVAVVPGDSFGSDDHVRISFATSMENIVKGMDRIEEA
                     VGKLS"
     gene            complement(3285..3788)
                     /gene="coaD"
                     /locus_tag="VFJ67_09860"
     CDS             complement(3285..3788)
                     /gene="coaD"
                     /locus_tag="VFJ67_09860"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET7290730.1"
                     /translation="MRKVAIYPGSFDPFTKGHQNIIERGVRVFGQVVVAVAHNTSKKT
                     VFTLQERVDLLNEIFRGRDDVRVDYFEGLLVEYAKKMGTNVVLRGMRTVSDFEYELQM
                     ALANKTLSPELETVFMVTDSEFSHISSSVIREVVSLGGQATHMVPGIVETKLREKLLK
                     GRRNRIK"
     gene            complement(3776..4336)
                     /gene="rsmD"
                     /locus_tag="VFJ67_09865"
     CDS             complement(3776..4336)
                     /gene="rsmD"
                     /locus_tag="VFJ67_09865"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET7290731.1"
                     /translation="MLHVLTGSHKGRKLKVPKGQKIRPTTSRVKKSIFDRLGDISGLR
                     VLDIFAGSGGLGIESLSRGASHVTFIEKDASVFKILIQNIADCGLREKATLICAGYEQ
                     ALKSLSRDKEGFDIIFMDPPYKLYETLKVGDFIESAAPLLNREGLIVIEHDHKISDTP
                     PGFTRFTKPFGGTHLSFFRRSDECVK"
     gene            4647..5669
                     /locus_tag="VFJ67_09870"
     CDS             4647..5669
                     /locus_tag="VFJ67_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290732.1"
                     /translation="MKKLRNLLMYAALVLAPSLLYVGCDGGGDGGGQNGQATTINGRV
                     ANVVAMNGHENDSFKFAELMKALSFIKEAKAQGGISVTALINGVVVDSDTTDSDGFFS
                     LTFLLDSAENVTLTFDIDGTIVSVSITVQEGSILNIIVTIDLNAPPGDEVDVIDEGEA
                     EGPVSCENGTLEITKNPGEDLIIDGGGEDCIRTAGNCNLIIDAENIFLINCENCIDAR
                     GTSDVSLGTFNGDIFCDASEDGIKTVGDATVLIDVNGDLNVSADENGAKAEGNSVITF
                     SANACIFDTGEDIFDTNGNATIDADGCGEIIEGPVVTPAPTLEPTPIPSPEPSPEPSP
                     EPSPEP"
     gene            5905..6306
                     /locus_tag="VFJ67_09875"
     CDS             5905..6306
                     /locus_tag="VFJ67_09875"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HET7290733.1"
                     /translation="MNCLTCRCRLPILIIVLATFTPVSLIASSVNAAKVESQMISPDK
                     NQPTPREIDELKVRRALENKIVAGRLMGHGLTTDEVSAKLNRMNDEQVHQMASLADKI
                     PAGGDAGLGIVIAILVVVALVLLIVFLFKRV"
     gene            6338..6892
                     /locus_tag="VFJ67_09880"
     CDS             6338..6892
                     /locus_tag="VFJ67_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET7290734.1"
                     /translation="MLNNCTLILTIFIVSLTLPGCAGNSTGGKGDPAVISGKVISGVP
                     FVKQKDQFCGPAAMASVLAYYGDNITQEEIAEKVYTPKLDGALISDMENYARDSGYKA
                     ETASGDITAIEKEIDEGVPVILLVDKGRWMVSVPHYYVVYGYDSRKGIFIVNTGDEGG
                     VKIPFERLDGEWEKMNRLMLIVKK"
     gene            6889..7476
                     /locus_tag="VFJ67_09885"
     CDS             6889..7476
                     /locus_tag="VFJ67_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7290735.1"
                     /translation="MKAPNPGFLALLFILSLISSGCASIYASYTAKDPLTAEEHNNLG
                     VIYEREGKYDLAIREYKRAASADGELVVPLVNLGNVYQKKGETKEAEKYYKKALRKDE
                     HNIEAANNLASLFIETGGDCGEALEILLAATAKENPAPAYALDTLGVLYFRTGNNGKA
                     KESLIEACTRASGDEELMKEIDAHLAELGDGAGCK"
     gene            complement(7511..8434)
                     /gene="fmt"
                     /locus_tag="VFJ67_09890"
     CDS             complement(7511..8434)
                     /gene="fmt"
                     /locus_tag="VFJ67_09890"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET7290736.1"
                     /translation="MRIVFMGTPEFAVPSLKALADSGEEVAAVVAQPDKPQGRGLKLS
                     PPPTKVFAGERGITVLQPPGIRTEAFLRDLAELNPDIIIVAAYGKILPKSVLELPGFG
                     CINVHASILPKYRGAAPVNWAIARGEKVTGVTIMRMDEGMDTGDILLTREVPIDYDDT
                     GETLSRKLSHAGAELLLTSLENLKRGELHPLKQNGSLATYAPMLKKEDGRIDWSKPAG
                     EIRNLIRGMLPWPGAFTFLGGKLLKIYKAAIAEGAGKEGEVITAPQGVLRVMTGKGAL
                     DILELQIEGGKRLPSRDFLSGRKIEPGTVLG"
     gene            complement(8579..9907)
                     /locus_tag="VFJ67_09895"
     CDS             complement(8579..9907)
                     /locus_tag="VFJ67_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET7290737.1"
                     /translation="MQKELRRYLFLGDFDSFLLGLFSKRGEVIWVSSLEEALNQTGNF
                     SVIMIESRSFSPELTKELSTHYPQTPMIVSNGEQKEEPPPSVRFVAFHKLLSDEIAYE
                     EKLARTDLRVDELRKVHANAKKLLILLHDHPDPDSIASALALRALLKRNKQTATIGHL
                     GDKISRPENVAMIELLDIDLQELQPEDLKDYDSIALLDVQPPFFGSELPPVDTVIDHH
                     PLVGSYEAKFKEIRTEEGATSTILTKYLRASQIDISERLATALLYGIKTDTVMLNRDA
                     DPDDVESFTFLYPLANLGLLRRIERAEVPPSEIKSFGKALADHWIVDNVFFVNVGRVD
                     REYLIPKMADFGIQVKGIEWSVAFGILANSHLIISVRNVGYVKSAGRLVRELFKEVGS
                     AGGHRSAAKAVISLKAVRKRIGKGSLEAIKKWLTEEFSRAVSEKAEENGH"
     gene            complement(9907..10740)
                     /gene="trpA"
                     /locus_tag="VFJ67_09900"
     CDS             complement(9907..10740)
                     /gene="trpA"
                     /locus_tag="VFJ67_09900"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7290738.1"
                     /translation="MSRIADKFNELKKKNRIALVNYVTAGDPSPEMTADLVLKLVESG
                     ADIIELGVPFSDPMADGPAIQLASERALKKGTTLRMVLDMVKRIRKKSDVPIILFGYY
                     NPIFKFGLERFSIDAARAGADGVLVVDLPPEEAGELKVHTDKAGLDLIFLLAPTSTGD
                     RIGLVAKHASGFVYLVSVTGITGIRPDMDYSLEDLTSEIKHHTKLPVGVGFGISSPEQ
                     VSRIAEYADAVIVGSAIVRIIEKHGEKKEELFDELSGFVSGLSRACGGKSEVSLNRAH
                     K"
BASE COUNT         2423 a         3058 c         2868 g         2556 t
ORIGIN      
        1 cttcgagcat gatcgccgca gtgaaagtcg catcgcggct cgcggctcag cctcataaaa
       61 gcggaaatta gcatcagctg aataccttcc tggatgtgta aaggagggga agtatttccc
      121 cggatttccc gagtatggtc atatcgaaga gcggcgagtt cggcgtgtac tcgaggttta
      181 tctcgaggac ggtctttcct gtctgcttcg cgaccaggcc catcgatgcc gcaggctcta
      241 cgatccccga tgtcccgata atgagcatca cctcgcactc ctctatagcc ttgagggaaa
      301 cgtctatcac gtgcatcggg attatctccc cgaaccagac gacgtttggc ctccctatct
      361 cgccgcagac gacgcacctc ggaggcagct cctcaagagg cgtcttgtgg ctctgctgaa
      421 tgtccccgca tttcgtacac cttatctgcc agatatttcc gtgcatctcg attacgtttc
      481 tgctgcccgc catctgatgg aggccgtcaa tgttctgtgt gataagggtg aacttcttca
      541 tctgaccctc gagctcggca agggcgaagt gccccgggtt gggctttgcg tcctttacgg
      601 cgctccttct ccagtcgtac cattcccata cgagcctggg gttattccag aaggcctccg
      661 ctgtcgcgag atcctcagat ctaaaattcc tccagagtcc gtccttgccc ctgaaagtcg
      721 gaacaccgct ttcggcggag atacccgcgc ccgtaagaac ggtgagagat gagcagttct
      781 ctattatatc ccttgcggct gatatcttct cgtgcatttc attcatgaaa taagtttaca
      841 gcatatactc agtaagcggg ctgcatgggg tagataaaac gggcttccga gaatctctca
      901 ggagaaacta tcaccttttt accgccctgc cactgaacga cgaccatttc atgcgcggtc
      961 tggaaaccgt tgccgtccgt cccgaactgc ccgtagaacg tcgtgagctt cttgccgcgc
     1021 gcgcttttcc tcagcgctcc gtcatcaagc gtccctgtat cctcgataca cttcctgact
     1081 atcaacccga tgttgtagcc ctgtgcagcg gtataatcgg ggtgtttccc gtatgcggac
     1141 ataaagcgcc cggcaaattc ccggggcgag ggccccgcat caggcgttac gccgattccc
     1201 tcctcccatt gactcgagga cgtgaaacct tccgcgctcc gcccgaacgt atccgcgaaa
     1261 agtttgatcg acgccgctac gaaagcggcc gcatttaccc tgacctttct ctctatgacg
     1321 tgtctcgcga gggcgatgtc gtcgtgcgca cgccccattc cgagaatcag gtcgggcgcg
     1381 aaaccgaggg cttcgtccag gagcccggaa aagtcttcgc tcccggagac gaattgaaat
     1441 tccctcatct caaaaccgtt ctcctgcgca gtgagccggg cgccgcgggc gacgttcctc
     1501 gaaaaaccgc tgtccacggc gctgaacgac gcgatctttc gagccgccgg gtcccgcgac
     1561 cttacaagct tgattattcc ttcagcgtat ctgactgttg gtgtgatcgc gttgacgaga
     1621 catgtgaatc ccctgccctc catctcgtcc gtcgagccgc cgtggttcca tagagtcacc
     1681 cccgctcctt ccgcaatttc cgccgaagcg agagcgaggg agctcgaata cgggccgaga
     1741 aggatgtctg ccttcctctc agcgatgagc ttttcggtgt tagcccggca tgtatcgacc
     1801 gagctgcggt cgtcgaggta tacgaggtct atgggcaggt tccttccgag ctcccttacg
     1861 aaaatgccgc cttctttatt tacgtcggat acccagagac tgataccctc gaagctctcc
     1921 ctgccctgga cggaatatgc tccggtgagg gatatagaca ggccgacttt tatactccgc
     1981 atcatctgaa tccgccgggg aggccacacg gacaaagctc tcccgcagca catagaatat
     2041 gctctatata ttactacgga tagagtggaa ttagcgagag accgattgcg cccttcagct
     2101 cagcttgccg accgcttcct cgatcctgtc cattcccttc acgatgtttt ccatcgacgt
     2161 cgcgaaggaa atcctgacat ggtcgtccga gccgaaagaa tcgcccggaa cgacggccac
     2221 cttggcttca tccagcagga actcggtgaa gctcatggaa ctgtttacat ccttcccgtt
     2281 gtaacgcttg ccgtagaaac cagacacgtt ggggaacgca tagaaagccc cctgcgggct
     2341 gaagcatttc atgccgggta ttgcattgag cctctcgaca atatagttct tcctcttctc
     2401 gaactcgcgg gtcctctcct cgatcacgtc gtgagggccc ctgtaggcct cgagcgcggc
     2461 ccattgggat atggacgtag gattcgacgt ggactgaccc tggagcttgc ccatcgcgtt
     2521 tatcacatcc ttgtcgcccg cggcgtagcc gatcctccat cccgtcatcg cgtatgtctt
     2581 cgagacgccg ttcacgagta tagtcgcttt cttcacgtcc tcgcccagtg tggcgacggg
     2641 cgtatgtttg atgccgtcga aaataatctt gtcgtatatc tcgtccgaga tgatcgtcag
     2701 ccccgcttcc atcgccacgt ccacgataag cctgagctcg acttcgttat atgtcgcgcc
     2761 tgtcgggttg gaagggtaat tgagcacgag cgccctcgtc ttcggagtta tcagcttctt
     2821 cagctcggcg ggatcgatct tgaacccctc ttcctccaga gtgtttagaa tcacggggtc
     2881 ggcgcctgcg agtgtaatct gctctggata cgacacccag taaggagcag gcactatgac
     2941 ctcgtcacct tcttcgaaca tggcctgggt gaggttatat agagtatgtt ttgcgccgtt
     3001 cgacactatt atttccggct tctggtattc gagcccgtaa tcaagcttca tcctgttgat
     3061 tatcgcttcc ttaagctcgt ctatgccgcc gaccgcggta tatttcgtaa acccggccct
     3121 gatagccttt atcgcttctt ctttaacatt ctcggggctg tcgaaatccg gctcccccgc
     3181 accgaatcct atgacgtcca caccctgagc tttgagggcc ttctcctttg cggtgattgc
     3241 gagagtcgcg gaaggctgca gctttttcgc acgctgagag agtttcattt aatccgattc
     3301 ctcctgcctt ttaatagttt ttccctgagc ttggtctcga ctataccggg gaccatatga
     3361 gtcgcctgcc cgccgagaga taccacttcc cttatcacgg aagagcttat gtgcgagaac
     3421 tcgctgtctg taaccatgaa gaccgtctcg agctcgggac tgagggtctt gttggcaagc
     3481 gccatctgaa gctcgtactc gaaatcagag accgttctca ttcccctgag cactacgttc
     3541 gtccccatct ttttcgcgta ttcgacgagg agaccctcga agtaatccac cctgacgtca
     3601 tcgcgtccgc ggaaaatctc gttcagcaga tcgactcttt cctggagcgt gaataccgtt
     3661 ttcttcgacg tattgtgggc gaccgcgaca acgacctgac cgaacaccct tacaccacgc
     3721 tcgatgatat tctggtgccc cttcgtaaac gggtcgaacg aacccggata gatggctact
     3781 ttacgcattc atcgctcctt ctgaaaaaac tgagatgtgt cccgccgaaa ggttttgtaa
     3841 accttgtgaa gccgggaggt gtgtcgctta tcttgtggtc atgctcgatc actatcaagc
     3901 cctcacggtt taatagagga gcggcgcttt ctatgaaatc cccaaccttg agcgtctcat
     3961 agagcttgta gggagggtcc atgaatataa tatcgaaacc ctctttatcc ctgctcaggc
     4021 ttttaagcgc ctgctcgtat cccgcgcaaa tcagcgtcgc cttctccctc aatccacagt
     4081 cagcgatatt ctgaatcagt attttaaata ccgaggcatc cttttcgatg aaagttacgt
     4141 gcgaagcccc gcggctgagc gattctatac cgagaccgcc cgagccggcg aatatatcca
     4201 ataccctgag acccgatatg tcaccgagcc tgtcgaatat cgattttttg accctgctcg
     4261 tggtagggcg tatcttctgc cccttcggaa ccttgagctt tctgcccttg tgggagcctg
     4321 ttagcacatg caacatgagg gttgatatac taccatagcg gccttttttg gcaaagaatc
     4381 ccggcacata aaaagagcgg ccgtcaacag ccgaatgaaa cccgatgtgc catgttgcac
     4441 agtacggggt gtacataaaa atacaatgat atagctcaga aacatttact aatgatcctt
     4501 tccccctgta attccagcga atcagcttat cgcccctgca ggcctcgaaa ttgcataccg
     4561 tgaatatgta tctgtatata tattgcagat acaataatta actgacgatc atggcaaaac
     4621 aaattacgca aggaggatgt tatacgatga agaaactgag aaatttactt atgtacgcag
     4681 ctctcgttct ggcgccgtcc ctgttatacg tgggatgcga cggaggggga gacggaggcg
     4741 ggcaaaacgg gcaagctacg actatcaacg gaagggtcgc gaacgttgtt gcaatgaacg
     4801 gtcacgaaaa tgattcattt aagttcgcag agctcatgaa ggccctaagc ttcataaagg
     4861 aagcaaaagc gcagggcggc ataagcgtaa cagcactcat caacggagtg gttgtggact
     4921 ccgacactac ggattcggac ggatttttct ccctgacctt ccttctcgac tcggctgaaa
     4981 acgtgaccct cacttttgac atagacggga ccatcgtatc ggtctccata acggttcagg
     5041 agggctccat actgaacata atagtgacga tcgaccttaa cgcccctccc ggggacgagg
     5101 tggacgtcat tgacgagggc gaagccgagg gcccggtcag ttgtgagaac ggaacgctcg
     5161 aaatcactaa aaacccgggt gaagacctga taatcgacgg cgggggagag gactgcattc
     5221 gcaccgcggg gaattgcaac ctcataatcg acgccgaaaa catcttcctt atcaactgcg
     5281 agaactgcat cgatgcaagg ggcacatccg atgtctcgct cgggacattc aacggagata
     5341 tcttctgcga cgcgtccgaa gacggcatca aaaccgtcgg agacgcaact gtattaatcg
     5401 atgtcaacgg tgatcttaac gtatctgcgg acgagaacgg agcgaaggcg gaagggaact
     5461 cggtcattac gttctcggca aacgcctgca tattcgatac tggtgaagat atattcgata
     5521 cgaacgggaa cgcgactata gacgcggacg ggtgcggcga gatcatcgaa ggcccggtgg
     5581 ttactcccgc gccgacgctc gagccgactc caataccgtc acccgagccc tcaccggagc
     5641 cgtcccctga gccctcgccg gagccatgaa acgcatgaat tccgacaatc ggtcataaat
     5701 tggggcgggt cagatagctc tgatccgccc ccgaatagta agtcccggca gactcatgac
     5761 tcgcataata atatcccgaa ggccggtgag ctcacagggg gccgcgtaga ccgcttccca
     5821 aacgaaattt tcattttaat tcgggaatat ataggataga attgtaatcg ctttttattt
     5881 tcaagcatcg gaggataaat acagatgaac tgtctgacat gcagatgccg cttgccgatt
     5941 ctcatcatcg ttctggctac gttcacgccg gtatcgctca ttgcctcgag cgtcaacgcg
     6001 gccaaggtcg aatcgcagat gatatcgccc gacaagaatc agccgacgcc gagagagata
     6061 gacgagctaa aggtcaggag agcgctcgag aacaagatcg tggccgggag gctcatgggt
     6121 cacggactta cgaccgacga ggtgtcagcg aagctgaacc ggatgaacga cgagcaggtg
     6181 catcagatgg catcgctcgc ggacaagatc cccgctggcg gcgacgccgg gctcgggata
     6241 gtgatcgcga tactcgtggt agtggctctc gtgctcctaa ttgtatttct tttcaaaagg
     6301 gtttagacag ccggagtatg gaatccgtgg attccgaatg cttaataact gcacactgat
     6361 tctcactata ttcatcgtgt cgttaactct tccgggatgc gccggaaaca gcacgggcgg
     6421 caagggagat ccggctgtaa tatccggaaa ggttataagc ggcgtgccat tcgtaaagca
     6481 gaaagaccaa ttctgcggcc ccgccgccat ggcatccgtg ctcgcatatt acggagacaa
     6541 tatcacgcag gaagaaatag ccgaaaaggt ctatacgccg aagctcgacg gagcgctcat
     6601 atcggacatg gagaactacg cccgcgatag cggctacaag gcggagacgg caagcgggga
     6661 cataaccgcg atcgagaaag agatcgacga gggcgtgccc gtgattttac tcgtggataa
     6721 ggggaggtgg atggtcagcg tcccccatta ttacgtcgtt tacggctacg attcgcggaa
     6781 aggaatcttc atcgtcaata caggggatga gggcggagtg aagataccgt ttgagcgtct
     6841 cgacggcgaa tgggagaaaa tgaaccgcct catgctgatt gtaaagaagt gaaagctccg
     6901 aacccgggat ttctcgcgct gttatttatc ctgtccctga tctcatcggg ctgcgcgtcg
     6961 atctacgcct cttacaccgc gaaagacccg ctcacagcgg aggagcacaa taacctgggc
     7021 gtgatctacg agagagaggg caaatacgac ctcgccataa gggagtacaa gagggccgca
     7081 tccgcggacg gagagctcgt cgtgccgctc gtcaatctgg gcaacgtcta tcagaagaaa
     7141 ggggaaacca aagaagcgga gaagtattat aaaaaggcgc tccgaaagga tgagcataat
     7201 attgaggccg cgaacaacct cgcgtcgctt tttatcgaaa cgggcgggga ttgcggggag
     7261 gcgctcgaaa ttctcctcgc tgcgacggcg aaagagaatc cggcgccggc ttacgccctc
     7321 gatactcttg gggtcttgta cttcagaacc ggaaacaacg ggaaagcgaa ggaatcgctt
     7381 atcgaagcct gcacccgtgc ctcgggcgat gaagagctga tgaaagagat cgacgcgcac
     7441 ctggctgagc tcggagacgg cgcgggatgt aaataaagcc cgctcctagg actcagggtt
     7501 tcccggattt ctaaccgagc acggttccgg gctcgatttt ccttcccgaa aggaaatccc
     7561 tcgaaggaag cctcttcccc ccctctatct ggagctcgag gatatcgagc gctccctttc
     7621 cggtcatgac gcgaagtacg ccctgagggg ccgttatcac ctcgccctct ttccccgctc
     7681 cctccgcaat cgcagcctta taaatcttga gcagtttccc gccaaggaat gtaaaagccc
     7741 cgggccaggg cagcatgccc ctgataaggt ttcttatttc accagccggc ttactccaat
     7801 ctatcctgcc gtcctctttt ttcagcatag gcgcgtaggt ggcgagagag ccgttctgtt
     7861 tgagtgggtg caattctccg cgctttaaat tttcgagcga cgtgaggagg agctccgcgc
     7921 ccgcgtgaga gagtttccgg gacagggtct ccccggtgtc gtcgtaatca atcgggactt
     7981 cgcgcgtaag aagtatgtcc cccgtatcca taccctcgtc catccgcata atcgtaaccc
     8041 ccgttacctt ttccccgcga gcgatggccc agttgacggg cgcagcgccc ctgtatttag
     8101 ggagtatgga cgcgtggacg tttatacatc cgaatcccgg gagctcgagc acggacttgg
     8161 gaagaatctt tccgtaggca gcgacgataa tgatatcggg attcaactct gcaagatccc
     8221 tcaaaaaagc ctcggttctt atacccggag gctggagtac cgtgatgccg cgctctcccg
     8281 cgaatacttt ggtcggcggg ggggaaagtt tgagacccct cccctgcggc ttatccggct
     8341 gcgctacgac ggctgccact tcctctcccg aatcggcaag cgcttttaac gagggcacgg
     8401 cgaactccgg cgtacccatg aatacgatcc tcatgcatct ggattatatt cacaaggctg
     8461 tgaggattca aaccggataa gcgatacaca gcgttcggga gattatttac agccaatagg
     8521 tgaaaaaata ttcaggggtc aagctgacac ggatgtattt tgggagtata gccggtaatt
     8581 aatggccgtt ttcttcagct ttctcggaaa cggccctcga gaactcctcg gtaagccatt
     8641 tctttatagc ttccagggaa cccttgccga tacgcttcct gactgccttc aaagatatga
     8701 ccgccttcgc cgcggacctg tgtcctccgg cgctgccgac ttccttgaac agctccctca
     8761 ctagccttcc ggcgctcttt acatagccta cgttccttac cgatattatg agatgagagt
     8821 tggcgagtat cccgaacgcg accgaccact ctatgccttt cacctgtatg ccgaagtcgg
     8881 ccattttcgg aatgaggtac tccctgtcca ccctgcctac attgacgaag aaaacattgt
     8941 ccactatcca gtggtcggcc agcgccttcc cgaatgactt gatctcggag ggcggaactt
     9001 cggcacgctc gatacgcctc agcaatccca ggttcgccag cggatagagg aacgtgaagg
     9061 attccacgtc gtccggatcg gcgtccctgt tcagcatcac cgtatccgtt tttattccgt
     9121 agaggagcgc ggtcgcgagt ctttccgata tgtcaatctg cgacgccctc aggtacttgg
     9181 tgaggattgt ggaagtggct ccctcctcgg tcctaatctc cttgaatttc gcttcatacg
     9241 agcccacgag cgggtggtgg tctatgaccg tatccacagg cggcagctcc gagccgaaga
     9301 acggcggctg cacgtcgagg agggctatcg agtcgtagtc cttgaggtct tccggctgaa
     9361 gctcctgcag atcgatatcg agaagctcta tcatcgccac gttctccgga cgcgagatct
     9421 tgtcccccag atgacctatt gttgcagtct gcttgttcct cttgaggagc gccctgagag
     9481 caagcgcgct cgctatggaa tcggggtccg ggtggtcgtg gaggagaatg agcaatttct
     9541 ttgcgttggc gtggactttc cgcagctcgt cgaccctgag atcggtcctg gcgagttttt
     9601 cctcgtatgc tatctcatcc gaaagcagct tatggaacgc cacgaaccga acggacggcg
     9661 ggggctcctc tttctgctcg ccgttactga ctatcatcgg cgtctgggga taatgggtgg
     9721 aaagctcctt tgttagctcg ggggagaagg acctgctctc gatcatgatt acggaaaagt
     9781 tgcctgtctg gttgagtgct tcctcgagcg agctcaccca gatgacctcg ccccttttag
     9841 agaaaagccc aaggaggaag gaatcgaaat cccccagaaa aagatatcgt cttagttcct
     9901 tctgcattat ttgtgagccc tgttcaggga tacctcgctc ttaccgccgc atgccctgct
     9961 caagccgctt acgaacccgg atagctcgtc gaacaattct tctttctttt caccgtgctt
    10021 ttctattatt ctgacaatcg cgcttccgac tatcaccgca tcggcgtatt cagcgatcct
    10081 ggatacctgt tcgggtgaag atatgccgaa ccctacgccg acggggagct tggtatgatg
    10141 cttaatctcc gaagtcaggt cctcaaggga ataatccatg tccggtctga tgccggtaat
    10201 gccggtcacg gagacgagat agacgaaacc gctcgcatgt ttcgctacga gaccgatcct
    10261 gtcccctgtg ctcgtcggag caaggaggaa gatgaggtcg agccccgcct tgtcggtgtg
    10321 gactttcaat tcgcccgctt cctcgggcgg caggtcgacg acgagcactc cgtccgcgcc
    10381 cgcccgggcg gcgtcaatcg agaacctttc gagcccgaat ttaaaaatcg gattgtagta
    10441 gccgaagagg attatcggga catctgattt tttcctgatg cgctttacca tatcgaggac
    10501 catcctgagg gtggtccctt ttttgagcgc ccgttcggag gcgagctgta tggcgggccc
    10561 gtcagccatc gggtcggaga agggtacgcc cagctcgatg atgtcggccc cgctctcgac
    10621 gagcttcagc accaggtccg ccgtcatttc gggcgacggg tcgcccgccg tcacgtaatt
    10681 gacgagcgca atcctgttct tctttttcag ctcattgaac ttatcggcta ttctgctcat
    10741 atgttttgga gttggaattt aagtagggtc agacgacagt aatttgtttc caaactacgg
    10801 ctcttggtga acgttattta acgacgtaaa aaagttatat gccaggaacc ccgcccgcat
    10861 ctgatactta agggacaatc taacacttcc gggactaaag tcaag
//
LOCUS       DASSJH010000450         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1289803, whole genome shotgun sequence.
ACCESSION   DASSJH010000450 DASSJH010000000
VERSION     DASSJH010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289803"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..706
                     /gene="tsf"
                     /locus_tag="VFJ67_09905"
     CDS             101..706
                     /gene="tsf"
                     /locus_tag="VFJ67_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET7290739.1"
                     /translation="MADITAQMVKEIRDRTGAPFIDCKKALEEVSGDFDKAIEILKIK
                     GVAKAAKKVGRETPEGIISSYIHAGGKIGVLVEINCETDFVARNDEFQAFAKEVAMQI
                     AAGNPRYISREYIPEDELAKEKEIMKAQVIESGKPANIADKIAEGKIEKFYEEVCLVD
                     QVYIRDTKLKINDLLQALIAKIGENIKIRRFVRFQLGEPLD"
     gene            718..1464
                     /gene="pyrH"
                     /locus_tag="VFJ67_09910"
     CDS             718..1464
                     /gene="pyrH"
                     /locus_tag="VFJ67_09910"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7290740.1"
                     /translation="MNSPKTKTGPRYRRILLKLSGEALQEPEGFGISSKALDAISDEI
                     VEVHGLGVEIAIVIGGGNIFRGVGGAETGIDRSTGDYMGMLATVINALALQDQLEKRQ
                     LNTRVQSALEIRQVAEPYIRRRAIRHLEKGRVVIFAAGTGNPFFTTDTAATLRALEIG
                     ADLILKATKVDGVYDKDPLKYKDAVKFKELTYMDVIQKELKIMDATAMSLCMQGNIPI
                     LVFNLFERDNIKKAVMGMDIGTKVRSDKDE"
     gene            1475..2029
                     /gene="frr"
                     /locus_tag="VFJ67_09915"
     CDS             1475..2029
                     /gene="frr"
                     /locus_tag="VFJ67_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET7290741.1"
                     /translation="MDEIIRETEDRMNKSLHVFDSELSKIRTGRASTALVEHISVDYY
                     GTMTPINQLATISTPEARTILIQPWDINAISAIEKAIMKSELGINPANDGKVIRIAIP
                     TLTEERRKDLVKHLHKLAEEYRVAVRQIRKDMNSKLKEAEKEKHVPEDAAKKNLNKIQ
                     EITDKFIQKINERLEKKEKDVLEV"
BASE COUNT          787 a          568 c          700 g          487 t
ORIGIN      
        1 gacacttatt cctacacaca tacggagaaa tcggaataag ccccgtatcg accgcggaga
       61 cagtctccag ggaagaaatc aaggaggttt ctaacaggaa atggcagata ttacggctca
      121 gatggtaaag gaaatcaggg acaggactgg cgccccgttt atcgactgca agaaggcgct
      181 tgaagaggtc tcgggtgatt tcgacaaggc tatagagata ctcaaaataa aaggcgtggc
      241 caaagcggca aagaaagtcg gaagggagac ccccgaagga atcatctcct cgtatataca
      301 cgccgggggg aagatagggg ttctcgttga aataaactgc gagacggatt tcgtcgcgcg
      361 caacgacgaa tttcaggctt tcgcgaaaga ggtcgcgatg cagatagcgg ccggcaaccc
      421 caggtacata agccgggagt acattccgga agacgagctc gcaaaagaaa aagagatcat
      481 gaaagcgcag gtaatcgaat ccggtaagcc cgccaatatc gcggacaaga tagcggaagg
      541 caagatcgaa aaattctacg aggaagtctg cctcgtagac caggtttaca tcagggacac
      601 gaaattaaag atcaacgacc tgctccaggc gcttatcgcg aagatcgggg aaaatataaa
      661 gataagaagg ttcgtaaggt tccagctggg cgaaccgttg gattaacgat cgaaaaaatg
      721 aattctccga aaaccaagac cggacccagg taccgaagga tcctgcttaa gctaagcgga
      781 gaggcccttc aggaacccga gggtttcggg ataagctcga aggcgctcga cgcgatctcg
      841 gacgagatcg tcgaagtaca cggtttaggc gtcgagatag ccatagtcat cggaggagga
      901 aatattttca ggggcgtggg cggggctgag acgggcatcg acaggtcgac cggggattat
      961 atgggcatgc tggcgaccgt cataaacgct ctcgcgctac aggaccagct ggaaaagaga
     1021 cagctcaaca cgagggttca gtccgcgctc gagataagac aggtagcgga accctatata
     1081 agaagacggg cgatcaggca cctcgagaaa ggaagggtcg tcattttcgc ggcaggcaca
     1141 gggaacccgt tcttcacgac cgacaccgcc gcgacgctga gggcgctcga gatcggagcg
     1201 gacctgatac tcaaggcgac gaaggtggac ggggtttacg acaaagaccc gctgaagtac
     1261 aaggacgccg tcaaattcaa ggagctcacc tacatggacg tcattcagaa agagctcaag
     1321 atcatggatg cgaccgccat gtcgctctgc atgcagggca acataccaat actcgttttc
     1381 aacctgttcg aaagggataa tatcaaaaag gccgtaatgg gaatggatat cggcacgaaa
     1441 gtcaggagtg ataaagatga gtgaagtgac aggtatcgat gaaattatcc gtgaaacaga
     1501 agacagaatg aacaagtcgc tacacgtgtt cgattcggag ctgtctaaaa taaggaccgg
     1561 acgggcatcg acggcgctcg tcgagcacat atcagtcgat tattacggaa ctatgacacc
     1621 tataaatcaa ctggctacca tttcgacccc ggaagcaagg acgatattga ttcagccctg
     1681 ggacataaac gcgatatccg cgattgaaaa agcgataatg aagtcggagc tgggaataaa
     1741 cccggcgaat gacgggaaag tgatcaggat agccataccc accctcacag aagagcggag
     1801 gaaggacctc gtaaagcatc tccacaagct cgccgaggag tacagggtcg cggtgagaca
     1861 gataagaaag gacatgaaca gcaaattgaa ggaagccgaa aaggaaaaac acgttcccga
     1921 agacgccgca aaaaaaaatc tcaataaaat ccaggaaatt acagacaaat tcatccagaa
     1981 aatcaacgag cggcttgaga agaaggaaaa agacgtcctg gaagtctaga atttggaccc
     2041 gggccctcca aacctcaccg cagtctttaa cactaggctt gaaccgcgcc aaaccagtaa
     2101 taattgtagg ttttgaggaa ttactctata attaaagcaa acaatccagt gagacggtaa
     2161 ggcgatgagg gattctctgt ccagcgtttt ctcctatcta ttcatggctg cggttattat
     2221 ctgtattatt tctttattcg gagcgcttat tatatttatg aggttttaca cgatggatat
     2281 agggggattg gaaaaacaga cggggtttgc gttcttgtac gtttttatat cttgcgtaat
     2341 cgccgccccg atttttcatt atatatccac caaacttgaa aaacatacga ggggtacgga
     2401 cggatattga ggagaaggat atcctctatc ggatggatgg ggtaacccgt aaaagcatac
     2461 gcctggcagc gggggattgc gatgtcacgg acctgtgccg cccgcttcag ccggaaagag
     2521 tcaacctctc aagcatcgga tg
//
LOCUS       DASSJH010000451         4779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_640666, whole genome shotgun sequence.
ACCESSION   DASSJH010000451 DASSJH010000000
VERSION     DASSJH010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640666"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..785)
                     /locus_tag="VFJ67_09920"
     CDS             complement(237..785)
                     /locus_tag="VFJ67_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007409512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7290742.1"
                     /translation="MKIFLLFLSLLIIQRVSELLLARRNERIVRDMGAFEHDGKGYRT
                     IVLMHAAFFVSLGAEYFLLHRTLNAYWLPLITLFLIAQALRYWAIASLGKFWNTKILV
                     VPGAQAVMKGPYRYLKHPNYIAVTIEIAAIPLLFSCYITCVLFSVLNLIALRRRIRIE
                     ESALYGARPGGGHGAPPSSAAG"
     gene            complement(782..1846)
                     /locus_tag="VFJ67_09925"
     CDS             complement(782..1846)
                     /locus_tag="VFJ67_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET7290743.1"
                     /translation="MSRIISVGTAVPQYQFRQSELKVFAKALLSENGYDPGRMLEVFD
                     TTEIDSRGFIHPREWFESGKSFRELSECYLSGALTLSRSAVVDCLNRYGGETGDFDHI
                     IYVTSTGVSAPTIDAVLLNEMKLDVHLKRTPIWGLGCAGGAAGLSRALEYTRANPKSA
                     VLLIATEICSLAFHGDDYSKSNMVSLALFSDGAAAVIVAGKENRLYGRSSLSLLDSLS
                     TTYYDSLDIMGWEVLDDGFKAIFSKDIPSIVRERVRGNIEELVSGVGISLDDLKHFIV
                     HPGGPKVLSEYERALGLDDGTFEYSRKVLREHGNMSSPTVLYVLREFMESGRPNGGEY
                     GIVSALGPGFSSELLLFRTE"
     gene            complement(2074..2355)
                     /locus_tag="VFJ67_09930"
     CDS             complement(2074..2355)
                     /locus_tag="VFJ67_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7290744.1"
                     /translation="MGCPYKAIADDTRRKILEFLSQGESAAGDIASKFDISKPAISNH
                     LKILKEAEIVNERKVRQNRLYSLNSNKIENMKFFLNSLTGKDGQSMRTQ"
     gene            2633..4108
                     /locus_tag="VFJ67_09935"
     CDS             2633..4108
                     /locus_tag="VFJ67_09935"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET7290745.1"
                     /translation="MEFRHSGESLLKVKADVLVLGIFKGEKFSGSSKSVNDALGDVLK
                     DLSRDEKFEGDMGKSVVLQSTFGKIGAKRVLLIGLGEKSRFSQDTVRKIGILAAAKYK
                     SACANIAFAPEFDGGKAYIAALAEGVMLGAYEFIKYKTKNGAESEIDRVIIVSKEEKE
                     RRFEGEIATANALADSITLARDMVNEPPLYMTPSKIADTASAVAQEGGMKCEIFDLEE
                     IKRRGMGGLAAVTSGSTEPPRFIHLTYEPRGKYKTTLAVVGKGITFDSGGLCIKPADS
                     MRTMKMDMAGAAAVLGIMKAVARLKPSVKVHGLISSCENMTGPAAYKPDDVIRAYNGK
                     TIEVINTDAEGRIVLSDALSYAVHLKVDEIVDLATLTGACMVALGAYTAGLMGNDQKL
                     IDRILSAAGSAGEKVWQLPMDDELRPEIRSDVADMKNAAGNRWGGAITAAMFLENFVG
                     DTAWAHIDIAGPAYNEKESGWNPKGGTGFGVRTLVSYIMDR"
     gene            4233..>4779
                     /locus_tag="VFJ67_09940"
     CDS             4233..>4779
                     /locus_tag="VFJ67_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET7290746.1"
                     /translation="MRGEIPEEEDKGFIPFFGGERRLTFGEIERILASIERSAGIAGV
                     IVSIGELRIGLSRANSLRRRLLTLRKNGKRVIVYIESGGNVEYLIASAGESVYMPPWS
                     MLNLVGLGAEVTFLKDLLDKLGVSARLKGYGEYKSAAETFTRSSISGAHREMVDSILG
                     SLRTQLEACISEGRGAKVADVR"
BASE COUNT         1169 a         1183 c         1326 g         1101 t
ORIGIN      
        1 ctgaatcatg gtaaaagacc ctcctcatgt gaattgtcag tttttaagga aaacttaaag
       61 aaaaaaacag gcggaacgag ctttctccgc cgcatattta tatagaaggc atataaaagc
      121 aatcagagca tatgaactaa gctatttatc ctttctatat agcatatcta atatatccct
      181 aaaattttat ataccgcagc tatccgggta atacgtccgc gataaaaacc ggggggttat
      241 ccggcggcgg acgagggcgg cgctccgtgt cccccgccag gccgggctcc gtacagggcc
      301 gactcctcga ttcttattct ccgcctgagc gctataaggt tcaatacgct gaacaggacg
      361 cacgtgatgt agcatgagaa taggagcggg attgccgcta tctctatcgt gactgcaatg
      421 tagttgggat gtttcaggta cctgtaaggc cctttcatta cggcctgcgc tccgggcacg
      481 acgagtattt tcgtgttcca gaacttcccg aggctcgcta tggcccagta cctgagcgct
      541 tgagcaatga gaaatagggt gatgagcggc agccagtagg cgttcaacgt cctgtgcaac
      601 aggaagtatt cggcacccag ggaaacgaag aacgcggcgt gcatgagaac tatcgtcctg
      661 taccccttcc cgtcgtgctc aaaggccccc atatccctga cgatgcgttc gttgcgccta
      721 gccagaagga gttctgaaac gcgctggatt atcaataaag ataagaagag caggaatatt
      781 ttcattctgt gcggaacagc agaagctccg agctgaatcc gggaccgagc gcggagacaa
      841 tgccgtattc ccctccgttc ggtcgcccgc tctccatgaa ttccctcagc acgtaaagca
      901 ctgtggggga ggacatgttc ccgtgctccc tcagcacctt cctcgaatac tcaaacgtcc
      961 cgtcatcaag gccgagagcc ctctcgtact cgctaagaac cttcggccct cccggatgga
     1021 cgatgaagtg tttcaggtcg tcgagcgata tgcctactcc ggaaacgagc tcctcgatat
     1081 tacctctgac tctttccctc acgatcgacg ggatgtcctt actgaagatg gctttgaagc
     1141 cgtcatccaa gacctcccac cccataatat cgagcgaatc gtaataggtc gtggacaggg
     1201 aatctagcag actcaggctc gagcgtccgt agagcctgtt ctcttttccc gccacgatta
     1261 cagccgccgc gccgtcggag aaaagagcca gtgaaaccat attgcttttg gaataatcgt
     1321 ctccgtggaa agcgaggctg catatttccg tcgctatgag gagcacagcg cttttcggat
     1381 tggccctggt gtattcgagg gcgcggctga gaccggcggc ccctcccgcg cacccgagcc
     1441 cccagatcgg ggtgcgtttc aaatgaacgt ccagcttcat ctcgttcagc agcacggcgt
     1501 cgatcgttgg ggcagagacc ccggtgctcg ttacgtagat aatgtggtcg aaatcccccg
     1561 tctccccgcc gtatctattc agacagtcta ccaccgccga gcggctgagg gtcagcgccc
     1621 ccgagagata acactcggaa agttcccgga agctcttccc gctttcgaac cactccctcg
     1681 ggtggatgaa tccgcggcta tcgatctctg tggtgtcgaa cacctcgagc atcctgccgg
     1741 gatcgtatcc gttttcggaa agaagggcct tggcgaatac cttcagctca ctctgtcgga
     1801 attgatattg cggcactgca gtgccaaccg aaattattct cgacattcga tcctgccttt
     1861 cacccgactt ggattcggta attcgctatt tgggatacat tcctgccggg tgtaccggga
     1921 tgggatacat taaacgtcct gttataagag tatataggta aaaagccgtt cccggtaatt
     1981 aaagtacggg gagcggcttt ttagtgaaga gtaacagatc gaggcagtta cattcttctc
     2041 gtcgtaaagc tgacaaatac tgaacagttc tgattattga gttctcatcg attgaccgtc
     2101 ttttcccgtc agggagttca ggaaaaattt catattctct atcttgttgc tgttgagaga
     2161 atatagacgg ttctgtctga ctttacgttc gttcacgatc tccgcttcct tgagaatctt
     2221 gaggtggttt gagatcgcgg gtttggatat atcgaacttg ctcgctatat ctccggccgc
     2281 gctttcccct tggctcagaa actccaggat cttccttctt gtatcgtctg caatagcttt
     2341 atacgggcaa cccatttcga cttcctcctt aactattatt ataggctttg ttagtgtatt
     2401 aatttacctt atctttgtcg atacttctac taaaaagtgc gcgcatctgt tttaaataaa
     2461 ttcttttaaa tttagagatt ttgaattccg tctcatgaaa attataccat aggatagaaa
     2521 gaaaattaat tctgatagtc aattatccaa aaaaatacca cggtttcaat ttcatgtctg
     2581 tatgataatc taattttaat ataccatatc gaactcgagg aggagcagcg aattggaatt
     2641 caggcacagc ggcgaaagtt tactgaaagt gaaggcggat gttctggtat tgggaatatt
     2701 taagggagaa aaattttccg gttcttcgaa gagcgtcaac gacgcgcttg gtgacgttct
     2761 taaagacttg tcccgggacg aaaagttcga gggggatatg ggaaagagcg tcgtgctcca
     2821 gagcacgttc gggaagatcg gagcgaaacg ggtgctgctg atagggcttg gtgaaaagtc
     2881 ccggttctcg caggatacgg tcagaaaaat aggcatcctg gctgcggcga agtataaatc
     2941 tgcatgtgca aacattgctt tcgccccgga gttcgacgga gggaaagctt atattgcggc
     3001 cctcgccgag ggggtcatgc tgggcgcata cgaattcata aaatacaaga caaagaacgg
     3061 ggctgaaagc gagattgaca gagtcataat agtatcgaaa gaagagaagg agaggagatt
     3121 cgagggagaa atcgccactg cgaacgcgct cgccgattcg atcactctcg cgagggacat
     3181 ggtgaacgag ccgcctctct atatgacccc ctcgaagata gccgatacgg cgtcggccgt
     3241 cgcacaggaa ggggggatga agtgcgagat tttcgacctc gaagagataa agaggagagg
     3301 catgggcggg ctcgcggcgg tgacgagcgg cagcacggag ccgcccaggt tcatacatct
     3361 cacgtatgag ccgcgcggga aatataaaac gacgctcgcg gtcgtaggga agggcataac
     3421 gttcgattcg ggtgggctct gtatcaagcc cgccgacagc atgcgcacga tgaaaatgga
     3481 catggcgggt gctgcggccg tgctgggaat catgaaggcg gtcgcacgtc tcaaaccctc
     3541 ggtcaaagtc cacgggctca tttcatcgtg tgaaaacatg acgggaccgg cggcatacaa
     3601 accggacgat gtaataaggg cttataacgg aaagacgata gaggtgataa acactgatgc
     3661 cgaggggagg atcgtgcttt cggacgccct ctcttacgcg gtgcacctga aggtggacga
     3721 gatcgtcgat ctcgcgacgc tcacgggggc ctgcatggtc gcgctcgggg cctatacggc
     3781 ggggcttatg ggcaatgatc agaagctgat cgacaggatc ttgagcgccg caggcagcgc
     3841 cggggagaag gtctggcagc tcccgatgga cgacgagctc cgcccggaaa taaggagcga
     3901 cgtcgccgac atgaagaacg ccgccggcaa caggtgggga ggcgcgataa cggcggcgat
     3961 gttcctcgaa aactttgtcg gggacacggc ctgggcccac atagacatag caggcccggc
     4021 atacaacgag aaggagagcg gctggaaccc caaagggggc accggattcg gcgtgaggac
     4081 cctagtctct tacatcatgg acagataacc aggtctgtgt gttccggatt atgcgccata
     4141 taaattaatc ctgttaaata tgcacttgcg ggttggagcg gatgcgcggc cttcttgaaa
     4201 cctttcgtaa atataatttt gtaaaactcg agatgagggg ggagatcccc gaagaagagg
     4261 ataagggctt tatccctttt ttcggtggcg aaaggaggct cacgttcggt gagatcgagc
     4321 gaattctcgc ctctatcgag cgttcggccg gtatcgccgg cgtgatagtc tcgataggcg
     4381 agctcaggat aggcctttcc cgcgccaact ccctcaggag acgccttctt actctaagaa
     4441 agaacggaaa gagggtcatc gtttacatag agagcggggg taacgtcgaa taccttatag
     4501 cttccgccgg ggagagcgta tatatgccgc cctggtctat gctcaatctg gtcggacttg
     4561 gagcggaggt cacctttctc aaggatctgc tggataaact cggcgtatcc gccaggctca
     4621 agggttacgg cgagtataaa agcgctgccg agacgtttac gaggagttcc atatccgggg
     4681 cgcacaggga gatggtcgat tccatcctgg gcagcctccg tacgcagctc gaggcctgca
     4741 tatcggaggg cagaggtgcg aaggtcgcgg atgtgaggg
//
LOCUS       DASSJH010000452         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_643876, whole genome shotgun sequence.
ACCESSION   DASSJH010000452 DASSJH010000000
VERSION     DASSJH010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643876"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="VFJ67_09945"
     CDS             complement(<1..1562)
                     /locus_tag="VFJ67_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7290747.1"
                     /translation="MFGRRLRHWAESVPDKTALIFRNKEGAYSRLSFRDFYRSCLQLK
                     STLAGMGFNQGDRIALYGENSINWVISYLSIHFLGATVVPLDSLFGAAEIINFLNFSE
                     SRAVLTDLPRAEKLKSEPAIMKSGATVLTIESLIDPPADHASAEPHTPGPDDLMVILF
                     TSGTTGIPKGVQLTINNVISTVSAILKSVDVTEDDNVLNILPLHHGYSSIVALLSPLF
                     AGATVTFSESLKSGDLLSAVRETGVTIFPGVPRLFEILYNEIEKRTARLPLVQKLIFS
                     GMRKISEFGWKRLGLRMGNIFFRQIHEPFGRRLRFFTSGGARLDPGILGGFLTLGFRM
                     AEGYGLTETTAVSTLTSPYKPVPGSVGKPLPGVEIKIDSPDADGVGEVCIKGPNITPG
                     YYKNEHATRELIRDGWLRSGDLGKIDPDGSLYITGRAKDVIVLPSGKNIYPEDVENLY
                     KDSPLIRELCVIPLKSPSGGLTGLRVIVVPEMKEMHERGIFGVGDRVRSIISMKGSHL
                     PSYMRISDVVLF"
     gene            complement(1649..>2068)
                     /locus_tag="VFJ67_09950"
     CDS             complement(1649..>2068)
                     /locus_tag="VFJ67_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HET7290748.1"
                     /translation="AAYAEGLGVLKSANIGKQKGEIDAETTPLRDPEHYQYDLNLTDV
                     TEVWRRGSVIASWLLDLTATALLQDPALSNFAGRVSDSGEGRWTIKAAIDEAVPVPVL
                     STALYARFSSRGDAEFQNRLLSAMRYEFGGHLEKPGK"
BASE COUNT          403 a          590 c          615 g          460 t
ORIGIN      
        1 ttgaataaga ccacgtccga tatcctcatg taagacggca ggtgtgagcc cttcatggag
       61 attatcgagc ggaccctgtc accgacaccg aaaattcccc tttcgtgcat ctctttcatc
      121 tccgggacca caatcacgcg gagcccggtg agtccgcccg agggggattt gagcggtatt
      181 acgcaaagct ccctgatgag ggggctgtct ttatacagat tctccacgtc ctcgggatag
      241 atgttcttcc cggacgggag cactatgacg tctttggccc tgccggtgat gtaaaggctg
      301 ccgtcggggt caatcttccc caggtctccc gagcggagcc acccgtccct gatgagctcg
      361 cgcgtggcgt gctcgttctt gtaatagccg ggcgtgatat tgggtccctt tatgcagacc
      421 tctcccacgc cgtcagcgtc cgggctgtcg attttgattt cgacacccgg gagcggtttc
      481 ccgacgctcc ccggcacggg tttatacggg gaagtgagcg tcgataccgc cgtagtctcg
      541 gtaagtccgt aaccttccgc catgcggaag cccagggtga ggaagccgcc caatatcccg
      601 gggtcgagcc ttgcgccgcc cgatgtaaaa aatctgagcc gcctgccgaa cggctcgtgg
      661 atttgcctga aaaatatatt ccccatcctc agaccgaggc gcttccagcc gaattcggaa
      721 attttacgca tcccactgaa gattagcttt tgcacgaggg gcaatcgggc ggttcttttc
      781 tctatttcgt tatacaggat ctcgaagagc ctgggcacgc cggggaatat cgtgacgcct
      841 gtctccctca cggcagacag caggtccccg cttttgagcg attccgagaa cgttacggtg
      901 gcccccgcga agagagggga caggagcgct acgatcgacg aataaccgtg gtggagcggt
      961 agtatgttga gaacgttgtc gtcctcagtc acgtcgacgg acttcaggat cgcgctcact
     1021 gtgctgatga cattgttgat cgtgagctgg acgcctttcg ggattccggt cgttccggag
     1081 gtgaacagta tcaccatcaa gtcgtcgggc ccgggtgtgt gaggttcggc ggacgcatgg
     1141 tcagcgggcg ggtcgatcag ggattcgatt gtcaagactg ttgcgccgga cttcatgatg
     1201 gccggctctg acttgagttt ctcagcgcgc gggaggtccg tgagtaccgc ccgcgattcg
     1261 gagaaattga gaaagttgat gatttccgcc gccccgaaga gagagtcaag aggcaccacg
     1321 gtcgctccca ggaagtgtat ggataggtag gagatgaccc agtttatcga gttctctccg
     1381 taaagggcta tgcggtctcc ctgattaaag cccatgccgg cgagagtcga tttgagctgg
     1441 agacagctcc ggtagaagtc ccggaaactc agccgggaat acgccccttc cttatttctg
     1501 aaaatcaggg cggtcttatc gggaacactt tccgcccaat gcctgagccg tcgtccaaac
     1561 ataattaccc ccgaagacgc atgtgataat ccggtttaga cgacgccgcg agctcggcgg
     1621 atcgcttatt caaacatgtg ccggcgcgtc atttacccgg tttctcaagg tggccgccga
     1681 actcataacg catggccgag agaagcctgt tctggaattc cgcgtcccct cgggagctga
     1741 accgcgcgta gagcgccgtc gacagaacgg gtacgggaac tgcctcatct atagcggcct
     1801 tgattgtcca gcgtccctcg cccgagtcgg aaaccctgcc ggcgaagttc gagagggccg
     1861 ggtcctggag cagcgccgtc gcggtcaggt cgaggagcca ggaggctatg acgcttcccc
     1921 gcctccacac ttcggtcacg tccgtcaggt tgaggtcgta ctgatagtgc tcggggtcgc
     1981 ggagcggtgt cgtctctgcg tctatctctc ccttttgctt gcctatgttg gccgatttca
     2041 gcacaccgag accttcggcg taagcggc
//
LOCUS       DASSJH010000453         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1077698, whole genome shotgun sequence.
ACCESSION   DASSJH010000453 DASSJH010000000
VERSION     DASSJH010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077698"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="carA"
                     /locus_tag="VFJ67_09955"
     CDS             <1..889
                     /gene="carA"
                     /locus_tag="VFJ67_09955"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET7290749.1"
                     /translation="VVKEYWREPSNWRSRGSLGSYLSENGIVGIEGVDTRSLTKLIRS
                     KGAQKAVISTTESDPGKLLKKVRSSQGIVGIDLVTEVSCDNPYKWSDGSGLWRPAAEG
                     TPGKGKRFRVVAYDYGLKQNILRKLVDHGCDVSIVPSRTPPHEILSMEPDGIFLSNGP
                     GDPAAVAYAVESVRTLIGKKPIFGICLGHQILGLALGGTTYKLKFGHRGANQPVKNLK
                     TGKVEITSQNHGFAVDAESLGGDVEITHINLNDNTVEGLRHREHPVFSVQYHPEASPG
                     PHDSSYLFGEFIDLMKEGS"
     gene            complement(1276..>2464)
                     /locus_tag="VFJ67_09960"
     CDS             complement(1276..>2464)
                     /locus_tag="VFJ67_09960"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HET7290750.1"
                     /translation="SFSSRFIELDAMNLAKEHGMEDLAVNAILLGAVSASGVLPIRRE
                     SFVQSVEMVGVAVKASLRAFEVGYEHVFSRKSPERKKPPVVWESFVQERADKLEEYDR
                     EEYLGRVSKLEAEFPPNLREILAESIFRLLEYQDSDYADEYIEDVRAVYEIDESAKGG
                     GFKLTENYAKNLALLMSYEDGIRVADLKIKSDRFKRIKEEMRLRDDQVFKVVDYLKPD
                     AEELYGLLPYFLVAPVVSLTETRLFKKIWRRKKPLTFAQTPVTTSFSGYARLWFLTKM
                     KFMRRHSFRYKKEHALIKKYTESVKYYASLDYRVGCLVAKSASMVKGYGKVRRRTMNA
                     FARFIDNVIFPIAEFEKNNRKNYDITLEIGEEALKLISGESVNGIEKAEKMAREVLER
                     RAA"
BASE COUNT          551 a          672 c          655 g          586 t
ORIGIN      
        1 cgtcgttaaa gaatattgga gagagccgtc gaactggcgc tccaggggaa gcctgggctc
       61 ctacctctcg gaaaacggca tcgtggggat agaaggggtc gatacgagat cactcactaa
      121 gctcatacgc tcgaaaggcg cccagaaggc cgtgatatcg actaccgaat ccgatccggg
      181 gaaactgctg aaaaaggtgc ggtcttccca ggggatagtg gggatcgacc tcgtgactga
      241 agtgagctgc gataatccct ataaatggag tgacgggagc gggttatgga gacccgccgc
      301 cgagggtacg cccgggaaag gaaaacgctt cagggtcgtc gcttacgact acggcctcaa
      361 gcagaacatt ctgagaaagc tcgtcgacca cggatgcgac gtatcgatag tgccgtcccg
      421 gacgcctcct cacgagatac tttcaatgga gcctgacggc atattcctct cgaacggccc
      481 cggggacccg gcggcagtgg cctacgcagt cgagagcgta aggacgctta tagggaagaa
      541 acctatattc ggcatctgcc tcgggcacca gatactcggc ctcgctctcg gcgggactac
      601 gtacaagctc aaattcggac accgcggggc gaaccagccg gtcaagaatc tgaaaacggg
      661 caaggtggag ataacttcac agaaccacgg gttcgcggtc gacgcggaga gcctcggcgg
      721 ggacgtggag attacgcaca taaacctgaa cgacaatacg gtcgaaggtc tcaggcacag
      781 ggagcatccc gtgttttcag ttcagtatca tcccgaggcc tcgcccggac ctcacgattc
      841 gtcttatttg ttcggtgaat ttatcgacct catgaaagaa ggcagctgag agacaccgcc
      901 ctttcactca ttcgcggcag ttcccctttt taaggagaag gaattagagg taatggggaa
      961 cgggtttgct atcgcggctg gaagccgctc ctacaaggtc gaggcgtata tactttttta
     1021 tgagcgcaag ttcctcggac ggccgcctgt acaattgatt caaattaaaa ggaccggatt
     1081 cccgatcaag tcgggaatga caggaagatt aaggaagcac gacccgttca acccttcgat
     1141 ccttcgactt cgttcaggac aggcgcgcag ttcaggattg tctaggctcc atatttagtt
     1201 aatgttggga gcgacagaag aacgtatacg agcgcaggtt ctacgcctca gggcgaacgg
     1261 ggttttgctg attattcagg ctgctcttct ttccaacact tcacgggcca ttttctctgc
     1321 tttttctatt ccgttcacag actcgccgga tatgagcttg agcgcctcct ctcctatctc
     1381 gagcgttata tcgtagttct ttctgttatt tttctcgaat tcggcgatcg ggaatattac
     1441 gttgtcgatg aaacgggcga aggcgttcat cgtcctcctc ctgaccttgc cgtagccttt
     1501 taccatggag gcgctcttcg cgacgagaca cccgaccctg taatcaaggg acgcgtaata
     1561 tttcacggac tccgtgtatt tctttatgag cgcgtgctct ttcttgtagc ggaaagaatg
     1621 ccgtctcatg aatttcattt tcgtgaggaa ccagagtctc gcgtatcccg agaacgacgt
     1681 ggtaacggga gtctgggcga atgtgagagg ttttttcctg cgccaaatct ttttgaacag
     1741 gcgagtttcg gtgaggctca caacaggggc tacgaggaag tatggaagaa gcccgtataa
     1801 ctcctccgcg tccggcttta aataatcgac gaccttgaat acctgatcgt ccctgagcct
     1861 catctcctcc tttatcctct tgaacctgtc cgattttatc ttgaggtctg ctaccctgat
     1921 gccgtcttcg tagctcatga gcagagcaag gttcttcgcg taattctccg tcagcttgaa
     1981 cccgcctccc ttcgcgctct cgtctatttc atagaccgct ctcacgtctt cgatatattc
     2041 gtcggcgtaa tcggagtcct ggtactcgag cagcctgaat atcgattcgg cgagaatctc
     2101 tctgagattg ggagggaact cggcctcgag ctttgagacc ctgcccaggt attcctccct
     2161 atcgtactcc tcgagcttgt cggccctctc ctgaacgaag ctctcccaga ctacgggagg
     2221 ctttttccgc tccgggctct tccgggagaa cacgtgctcg tatccgactt cgaacgccct
     2281 taaactcgcc ttcaccgcaa cccccaccat ctcgaccgat tgtacgaaag attctctcct
     2341 gatcggcagt acgccggaag cggagaccgc gcccaggagt atcgcgttga cagcgagatc
     2401 ttccatgccg tgctccttgg ccaggttcat ggcgtctaac tctatgaatc tcgatgaaaa
     2461 cgag
//
LOCUS       DASSJH010000454         7677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_968076, whole genome shotgun sequence.
ACCESSION   DASSJH010000454 DASSJH010000000
VERSION     DASSJH010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968076"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1909)
                     /gene="mrdA"
                     /locus_tag="VFJ67_09965"
     CDS             complement(101..1909)
                     /gene="mrdA"
                     /locus_tag="VFJ67_09965"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7290751.1"
                     /translation="MQDFRNRLVIATVILGIAFLALVSRLWFLQVLKGDEFEAFSRDN
                     RVRIERVPASRGRILDRYGRELVVNRPSFDVYVLPKDVDDVSALSVNLSKALGVEPEK
                     IERIITQASKKSRFKPVLLAQDIDRDQLAYIEARRSSLPGLTIEVNNLRQYPLGILGA
                     SFLGYIGKVSEAELLANPDLSNNGLVGKTGIEKTWEHSLRGKDGFTQKVTDALGREVN
                     SNLFQKDLVNRQSIPGNDIVLSVDIDLQKAAEDALGEEFGAIIVVNVKNGEVLALASK
                     PLFDPADFVKGIDAATWTRLINDKNFPLVSRATQGLYAPGSVFKMVPSAAALKEGVID
                     PDALVHCPGYYRLGSHVYRCWKKGGHGWMNMREAIVQSCDVYFYKLAEKLGIDRMSAY
                     MKYFGFGSPTGIGIEERAGVAPGREWKLEKFKKPWYKGETIVSGIGQGYVSVTPLQVS
                     MMTAAIANGGTLLTPKLVLKEVAFSGETKLNNVPPKGEVPVGKEMLALIRDAMTGVVN
                     GPGGTGHGARLENVTVAGKTGTAQVISLDSQSADKAHKDHAWFTSFAPAEAPEIAVTV
                     LVEHGGKGGAVAAPLAKKIIETYFQLKKERKANDNV"
     gene            complement(1890..2381)
                     /gene="mreD"
                     /locus_tag="VFJ67_09970"
     CDS             complement(1890..2381)
                     /gene="mreD"
                     /locus_tag="VFJ67_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET7290752.1"
                     /translation="MNKTLTFITLILVSILLITVQSTLLSPGRGGYLFPDLNLIFIVF
                     LAVYSETKGSFLIAAGNGYMMDVLSGNLPGTFTISRLSAYLLVRTSSSHVYLRKVIVQ
                     GLVIFLTTIFTWTFIMTIIRIKSNAGFHLTFGEIMLQGLVNSVVGVPLFWAAGRMHAR
                     LQK"
     gene            complement(2374..3201)
                     /gene="mreC"
                     /locus_tag="VFJ67_09975"
     CDS             complement(2374..3201)
                     /gene="mreC"
                     /locus_tag="VFJ67_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET7290753.1"
                     /translation="MGFFKRRAILVVLVLFFIAIQLFSLDLRERKSQNPASRFVLTLT
                     YYPQKLISTVTDKVAGTWRDYIDLVHVNEENKRLREELNALRQQKFELAEIEAQNTRL
                     KKLLDFKDVSPYPVVSANVIGASPSILRSQVVIVDKGRSDGITEGMPVASYDGIVGRI
                     LLAGDKSSEVLLITDPVSAVDAYIHRTRARGIVKGVGNGCVMEYIENKSDVSVGDKVI
                     SSGKDGFFPKGVIIGTVTDISPSGSFISAGISPHVDLNSLEEVVVIQKSVNNVVMNE"
     gene            complement(3255..4298)
                     /locus_tag="VFJ67_09980"
     CDS             complement(3255..4298)
                     /locus_tag="VFJ67_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET7290754.1"
                     /translation="MIFDTVLGWFSNDLAVDLGTANTLVYVRGKGIVANEPSVVAVQE
                     DSRGIKKILAVGKEAKDMVGRTPGSIKAIRPLKDGVIADFDVAQKMLEYFIRKTHNNR
                     KSFVRPRIIVSVPIGITEVEKRAVRESAEAAGAREVYLIEETMSAAIGAGMPVTEPTG
                     NMVVDIGGGTTGVAVISLAGIVVSKSIKVGGDKLDEAILQYVKRNYNMLIGERTAEDV
                     KKKLGKVLGNGDIGSMNVKGRDLRAGIPRTIEITSEEVKEALTEPVSLIIEAIKQTLE
                     RTPPELAADIVDSGIMLTGGGALLGNLDQLIKEETGLPVTRAEDPLTCVVYGSGKALE
                     ELDLLREVSIGYS"
     gene            complement(4483..5595)
                     /locus_tag="VFJ67_09985"
     CDS             complement(4483..5595)
                     /locus_tag="VFJ67_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET7290755.1"
                     /translation="MSIGRLPLHGVHESLGAGFEELGGSLVPANYGDTAGEYEAARSR
                     VGISDLSDRGKLRLSGKDHLKFLQGMLTNDVMKLGEGEGMYAAILTVKGRMLSDMRVY
                     REKESVLLDLEPGLNMKVGEILKGYRLSYKANIDDLTESIGLLYVHGPLARRLLVEAT
                     GVENLPQSEFGHLRAAIDGRDVTVVKVSRTGGEGYDVYATADVLSDIWNVLTENGKAH
                     GIVPVGRAAIETLRIEAGIPVYGTDMDEETIPIEAGIWNALSFEKGCYVGQEVVARIK
                     WRGHVNRHLSGLVLSGEMIPRKGSGIFSGEKKIGFVTSAVLSPAMAEPIALGYVRREY
                     IEPGTSVLIKLPDDSLGEARVTPLPFAGAKGVYRPE"
     gene            5810..6766
                     /gene="trxB"
                     /locus_tag="VFJ67_09990"
     CDS             5810..6766
                     /gene="trxB"
                     /locus_tag="VFJ67_09990"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7290756.1"
                     /translation="MADKKDIEGRHKVVIMGSGPAGLTAALYTARANLEPLVFEGMEA
                     GGQLTLTTEVENFPGFPDGILGPELMNSMRKQAERFGAKCVLSSITSVDLSARPFKIT
                     VDGKVIEAETFIIASGASAKMLGLESEKILLGHGVSTCATCDGFFFRDKELIVVGGGD
                     SAVEEAGFLTKYASRVTIVHRRDQLRASKIMQDRALKNPKVGFIWNAEVEEILGDKEG
                     GVRGVRLRDTKTGEVYEKAAYGVFVAIGHNPNTKLFKGMLEMDPVGYLVTKDGSTETS
                     VPGVFAAGDVKDSKYRQAITAAGSGCMAAIDAERFLEEHSGH"
     gene            6887..>7677
                     /locus_tag="VFJ67_09995"
     CDS             6887..>7677
                     /locus_tag="VFJ67_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290757.1"
                     /translation="MTLHFGEDREGKREHPLSAKSLVILCGLALAGLFVILSFLSDHF
                     SPETGLVKRPVLALVLIMTLSGAIYLFAIVKALNSINARKMLVFVLFTGAVLRLTMFF
                     STPILEDDYNRYLWDGAVTANGMNPYKYSPKEVIQNKDVPGELTGLAGDSGDIIRRIN
                     HPHIRSIYPPVTQALFALSYMIKPWSLLPWRAILLIFDIASLALILYSLRLTGLPLIY
                     SIIYWWNPLLVKEIYNSAHLDVLVLPFALGGVILAANTRYAGSML"
BASE COUNT         1650 a         2197 c         2036 g         1794 t
ORIGIN      
        1 tgtctgatag gacgcgctgt aaaggttcat cagagagagc gccgcgaacg cgagagtcat
       61 gaggaaaaga gaccacccga agctctggag gagcctcctg tcaaacatta tcatttgctt
      121 ttctctcttt cttcagctgg aagtaggttt ctataatctt ttttgccagc ggggccgcga
      181 ctgctccgcc tttaccgccg tgctccacga gcacggttac ggcgatctcg ggggcctcag
      241 cgggggcgaa tgaagtgaac caggcatggt ccttgtgagc cttgtcggca ctctgggaat
      301 caagcgatat gacctgcgca gtccccgtct tcccggcgac cgtgacattc tccaacctcg
      361 ccccgtgacc cgtacctccg ggaccgttaa cgaccccggt catggcgtcc cgtatgaggg
      421 cgagcatttc ctttccgacg ggaacctcac ccttgggggg cacattattg agcttcgttt
      481 caccgctgaa agccacttct ttcaggacga gcttcggtgt aagcagcgtg ccgccgttcg
      541 ctatggccgc cgtcatcatc gagacctgga gcggggtcac gcttacgtag ccctggccga
      601 ttcccgaaac tatcgtctcg cccttatacc agggcttctt gaatttctct aatttccact
      661 ccctgcccgg ggccactccc gcccgctctt cgatccctat gcctgtgggc gatccgaagc
      721 cgaagtattt catgtacgcc gacatgcggt cgatcccgag tttttccgcg agcttgtaga
      781 agtagacgtc acacgactgc actatcgcct cccgcatatt catccagccg tgccctccct
      841 tcttccagca cctgtaaacg tgagagccca gtctgtagta gccggggcaa tgcacaaggg
      901 catccgggtc gatgacccct tctttgagcg cagccgcgct gggcaccatc ttgaagacgg
      961 agcccggggc gtaaagaccc tgggtcgcgc ggctcacgag cgggaaattc ttgtcgttta
     1021 tcagtctcgt ccaggtagca gcgtctatgc ctttcacgaa atcggccgga tcgaacaatg
     1081 gcttgctcgc gagggcgagc acctcgccgt tctttacgtt taccacgata atggcgccga
     1141 attcttcacc aagggcgtct tccgcagctt tctggagatc tatgtctacc gataatacga
     1201 tatcgttacc cgggatgctc tgcctgttta cgaggtcctt ctggaagagg ttcgaattca
     1261 cttccctccc gagcgcgtcc gtaactttct gtgtgaaccc gtccttcccg cggagtgagt
     1321 gctcccacgt cttctcgatt cccgttttcc cgacgagacc gttgttgctg agatcggggt
     1381 tcgcgagaag ctccgcctcg ctcacctttc ctatatagcc cagaaacgaa gcgccgagaa
     1441 tgccgagcgg gtactgcctc agattattca cctctatagt gagaccggga agagagcttc
     1501 tacgcgcctc tatgtaggca agctggtcgc gatcgatgtc ctgggccagg agcacgggct
     1561 tgaaacggct ctttttcgac gcctgggtta ttatccgctc tatcttttcg ggttctacgc
     1621 cgagcgcttt cgagagattc acgctcaggg cggagacgtc gtccacgtcc ttgggcagga
     1681 cgtacacgtc gaaggacggc ctgtttacga cgagctcgcg gccgtacctg tcgagtatcc
     1741 tccccctcga agccggcacc ctctcgatcc ttacacggtt gtcgcgggaa aaagcctcga
     1801 attcgtcccc cttaagcacc tgaagaaacc agagcctcga gacgagcgca aggaacgcga
     1861 tgccgaggat cacggtcgcg attacgagtc tatttctgaa gtcttgcatg catccttcca
     1921 gccgcccaga atagcggaac gccgacaacc gaattcacaa gcccctgaag cataatttcg
     1981 ccgaaagtga ggtgaaagcc cgcgttactc tttattctaa ttatagtcat gataaaagtc
     2041 cacgtaaaaa tagtcgtcag gaatatgacg agcccctgga ctatgacttt cctgagatat
     2101 acgtggctgg agctcgtccg cacaagcagg taagcgctca ggcgggagat agtgaaggtc
     2161 cccggcagat tcccggagag cacgtccatc atataaccgt tccccgcggc aatgaggaag
     2221 cttcccttcg tctcagaata cacggcgaga aaaacgatga atatcagatt gaggtccggg
     2281 aaaaggtatc cccctcttcc gggggagagc agagtggact gtaccgttat caggagtatc
     2341 gagacgagta taagggttat gaaagtaagt gtcttattca ttcataacca cgttattgac
     2401 ggatttttgt attaccacta cttcctcgag cgagttcaga tctacgtgtg gagagatacc
     2461 ggcgcttata aagctcccgc tcggacttat atcggttaca gtgccgatta tgacaccctt
     2521 ggggaaaaac ccgtccttac cggaggagat gaccttgtcg cccacgctga catcggattt
     2581 gttctcgata tattccatga cgcatccgtt ccccacgccc tttacaatcc cccgcgcccg
     2641 cgtcctgtgt atgtaggcgt cgaccgcgct cacggggtcc gtgatgagga gtacctccga
     2701 gcttttatcg ccggcgagca agatcctgcc gactatcccg tcgtatgaag cgacaggcat
     2761 gccttcagtg atgccgtcgg atctgccctt gtcgactatc acgacctggg agcggagtat
     2821 cgaggggcta gcgcctatga cgtttgcgga cacaacaggg taaggggaca cgtccttgaa
     2881 atcgaggagc tttttgagtc tcgtgttctg ggcttcgatc tcggccagct cgaacttttg
     2941 ctgcctcagc gcgtttaact cctcgcggag cctcttgttc tcctcgttca cgtgcacgag
     3001 atcgatgtaa tcccgccagg tgccggcgac tttgtcggta accgtggata taagcttttg
     3061 gggataataa gtgagcgtga ggacgaatct cgatgccggg ttctggctct tcctttccct
     3121 gagatcgagc gaaaaaagct gtatcgcgat gaagaagaga acgaggacta ccagtatggc
     3181 gcggcgtttg aaaaaaccca tgtctatatc atatcagcaa atgaattata gtgcacagga
     3241 ttcgcggcct gttttcagga atatcctatg ctgacctctc tcaaaaggtc gagctcctcg
     3301 agcgccttgc ccgagccgta aacgacacag gtgagagggt cttccgcgcg cgttacggga
     3361 aggccggtct cctccttgat cagctggtcg aggttgccga ggagagcgcc ccctccagtc
     3421 agcatgatac cgctgtccac tatgtccgcc gcaagctcgg ggggtgttct ttcgagtgtc
     3481 tgcttgatcg cctctatgat gagacttacg ggctctgtga gggcctcttt gacctcttcg
     3541 gaggtaattt cgattgtgcg cggtatcccg gccctgaggt cccggccctt cacgttcatg
     3601 gacccgatgt cgccgttccc caataccttg ccgagcttct tcttgacgtc ctcagccgtc
     3661 ctctcgccga tgagcatgtt gtagttcctt ttcacgtact ggagtatcgc ttcatccagc
     3721 ttatcgcccc cgacttttat ggacttactg accactatcc cggcgagcga tattacggca
     3781 acccctgtgg ttccgccgcc tatgtccacg accatgttcc ctgtaggctc tgtaacgggc
     3841 ataccggccc ctatcgcagc cgacatggtc tcttctatga gatagacctc cctcgcgccc
     3901 gccgcctcgg ccgactccct gaccgccctt ttttccactt cggtgatgcc tatcggaacg
     3961 gacacgatga tcctcggcct gacgaaactt ttcctgttgt tgtgggtctt tctgatgaaa
     4021 tattcgagca ttttctgagc gacgtcgaag tccgctatta cgccgtcctt aagcggtctt
     4081 atagccttta tcgagcccgg cgttctgccg accatgtctt tggcctcttt gccgaccgcc
     4141 aggatttttt taatacctct cgaatcctcc tgcaccgcga cgaccgaagg ctcgttcgcg
     4201 acgatgccct tccccctcac gtacaccagt gtgttagccg tcccgagatc gacagcgagg
     4261 tcgttcgaga accagcccag aactgtatcg aatatcattt ccgagaccct cctacccaaa
     4321 ttatagaaca gaaattaata aaatgttaat ggtgatttat aaaagatacc caaccctatt
     4381 cattaactag ctttttgacc gagtcaaaac acccgactga tactataata gagagggaga
     4441 taaaatcaag gattacgggc ggtaattttt aatcctgggc cgctattccg gtcgataaac
     4501 gcccttagca cccgcgaaag ggagtggtgt aaccctggcc tccccgagag aatcgtcagg
     4561 gagcttaatc aggacggacg tgccgggctc tatatattcc cttctcacgt atccgagagc
     4621 tatcggttcc gccatggccg gggacagcac cgcgctcgtc acgaatccga tctttttctc
     4681 gcccgaaaat atcccggagc ctttccgggg tatcatctca ccagataata caagccctga
     4741 gagatgcctg ttcacgtggc cccgccactt tatcctcgca actacctcct ggccgacgta
     4801 acaccctttc tcgaagctga gcgcattcca tatgcccgcc tcaataggga tcgtctcttc
     4861 gtccatatcc gttccgtaaa cgggtatgcc ggcttcgatc ctgagcgttt cgatagctgc
     4921 cctgcctaca ggcacaattc catgcgcctt gccattctct gttaagacat tccagatatc
     4981 ggagaggacg tcggcagtag cgtatacatc gtagccctcc ccgcccgtcc tgctcacctt
     5041 aacaacggtc acatcgcggc cgtctatcgc cgctctcagg tggccgaatt cgctctgagg
     5101 caggttctct acgccggttg cctcgacaag gagcctacgc gcgagcggac cgtgaacgta
     5161 tagaagccca atgctttcgg tcaggtcgtc tatatttgct ttgtatgaga gtctgtatcc
     5221 cttgagtatc tctccgactt tcatattcaa cccgggctcg agatcgagca gtacggattc
     5281 cttttccctg tagacacgca tatcggaaag catcctgccc tttacagtga gtatagcggc
     5341 gtacatacct tcaccctcgc cgagcttcat cacgtcgttc gtgagcatgc cctgaaggaa
     5401 cttgagatga tcctttcccg agagacggag cttcccgcgg tcagacagat ccgagatgcc
     5461 gacacggctc ctcgcggcct cgtactcgcc ggcagtgtca ccgtaattcg ccgggacgag
     5521 cgagcctccc agctcttcga atcccgcccc cagggattcg tgtacgccat gtaatggcag
     5581 cctgccaatg ctcatatgaa tttttgagtt ctccccgaat attgaaattt atagttattc
     5641 taccagaagc gcagggctga gccgcgcgat atgccggtga atctttcttt tacgcccgtg
     5701 tatctaaata cagatcgata atatgtgaga cggggatatc ttgaaaatcg tctccccgta
     5761 tgtagtataa agcggggttt aggtaagacg catagattca ggagcggaga tggcagacaa
     5821 aaaggatata gaaggaaggc acaaggtcgt aattatgggt tcggggccgg ccggattaac
     5881 agcggctcta tatacggcga gggccaacct cgagccattg gtgttcgagg gtatggaggc
     5941 aggcggccag ctcacactta cgacagaggt ggaaaacttc ccgggtttcc cggacgggat
     6001 acttggtccg gagctcatga attcaatgag gaaacaggcg gagaggttcg gggcgaagtg
     6061 cgtgctgagc agcataacgt ctgtcgatct atccgcgagg cctttcaaga taacagttga
     6121 cggtaaagtg atcgaggccg aaacgttcat catagcctca ggtgcgtcgg ccaagatgct
     6181 gggactcgaa tcggaaaaga tactgctcgg tcacggcgtc tcgacctgcg ccacgtgcga
     6241 cggcttcttc ttcagggata aggagctaat cgtggtgggg ggcggcgaca gcgcggtcga
     6301 ggaagccggt ttcctcacta agtacgcttc gagggtgacg atagtccaca ggcgggacca
     6361 gctgagggcg tccaagatca tgcaggacag ggcattaaag aacccgaagg tcggtttcat
     6421 ctggaacgcg gaggtggagg agatactcgg ggataaggaa ggcggtgtga gaggcgtgag
     6481 gctcagggac acgaagaccg gcgaggttta tgagaaggcc gcctacggcg ttttcgtcgc
     6541 cataggccac aacccgaaca cgaagctctt caagggcatg ctggagatgg acccggtcgg
     6601 atacctcgtt acgaaagacg ggagcaccga gacgagcgtc cccggagtgt tcgccgcggg
     6661 cgacgtgaag gacagcaaat acaggcaggc tatcacggct gcggggtcgg ggtgtatggc
     6721 ggctatcgac gccgagaggt tcctcgaaga gcactccggg cactaatgag aaatctccgc
     6781 tccgaatccg tccgattacg attaatccga ataaaaacag tccgctccgg ccgccccgta
     6841 aagcgcgtat attaataccg ggtagagagt acccgggagc gtaacgatga ccctccactt
     6901 cggagaggac cgggaaggga agcgcgaaca cccgctaagc gccaagagcc tcgtcatact
     6961 gtgcggcctc gcgcttgccg ggctcttcgt tattttgtca ttcctctcgg atcatttttc
     7021 acccgagacg gggctcgtaa agaggcccgt actcgcgctc gtccttataa tgaccctttc
     7081 gggagctatt tatctattcg caattgtaaa agcgctcaac tccataaacg cccgaaagat
     7141 gctcgtcttc gttcttttca caggcgccgt gctgaggctc acgatgtttt tctccacccc
     7201 tatactcgaa gacgattaca acagatacct gtgggacggg gcggtgacgg cgaacgggat
     7261 gaacccgtat aaatattcgc ccaaagaagt tattcaaaat aaagacgtcc ccggcgagct
     7321 gaccgggctc gcgggggatt cgggcgacat aatacgccgc atcaaccatc cgcacatacg
     7381 gagcatatac cctccggtca cacaggcgct attcgcgctc tcttacatga tcaagccgtg
     7441 gagcctcttg ccgtggcggg cgatactcct gatattcgac atcgcctcac tcgccctaat
     7501 tttgtattca ctcaggctca cggggcttcc ccttatatac tccatcatct actggtggaa
     7561 tcccctgctc gtgaaggaga tatataactc ggctcacctg gacgtgctcg tcctcccgtt
     7621 cgcgctcggg ggcgtgatcc tcgctgctaa tacgagatat gcgggttcca tgttgag
//
LOCUS       DASSJH010000455         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_968888, whole genome shotgun sequence.
ACCESSION   DASSJH010000455 DASSJH010000000
VERSION     DASSJH010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968888"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..660)
                     /locus_tag="VFJ67_10000"
     CDS             complement(85..660)
                     /locus_tag="VFJ67_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290758.1"
                     /translation="MRISLLLFSLLFIAFAFYGCAKPKGNTPVSQRAYILQMRDNTLA
                     EIYQKSPYMQDLVQHAAGYGVFSNINTQLLILGSGNGYGVITDNSNGKTTFMRMAQGG
                     VGLGVALTDFRELIIFNNPAVMRQFISSGWDFGVQGDAVLKSKTKGSAASGQVPFDSD
                     IIIYQLTKQGIALRTNVGAEKYWIDSDLNYY"
     gene            886..2247
                     /locus_tag="VFJ67_10005"
     CDS             886..2247
                     /locus_tag="VFJ67_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HET7290759.1"
                     /translation="MNPGKNSGIVIFTSVIAALPGLMLGYDTGSVSGALGFISNTFRL
                     DTIGKGGIVSMVLIGGITGAFVNGFLADRLGRKITVQAAALLFIAGAAGSAFSPTAGA
                     LLASRFVLGLALGAVSASAPLYIAEISPAAGRGRLVTYFQLAITIGILLGYIVDRALT
                     GSGDWRLMLGTGAIPAFVLLIGMFVLPPSPRWLIKMGREDEARETLIKITGGDAVLAD
                     SELNDIKKVLSMESDGGFADMLRPPVSRAFVTAIGLFLTQQFVGINSIMYYAPSLFRG
                     AGMESQHSAILATVSVGVVNVLATFIAVWLIDRIGRKPLLYAGLAGMLISLSAIGLIF
                     ALGERGGYEIGRAAIIAVWFYIACFAFSLGPVPWIVMTEIFPLNVRGRAVSMATMSSW
                     GANLVVSFSFLPLLEAIGASYTYWLYAFISLIGIAFVWRFVPETKGMTLEEIEERLLS
                     GKK"
     gene            complement(2282..2665)
                     /locus_tag="VFJ67_10010"
     CDS             complement(2282..2665)
                     /locus_tag="VFJ67_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7290760.1"
                     /translation="MSDDKYKRGLELLEKVSPGASKRTAESLNDIAPDMVRYMMEFVF
                     GEVSSRPGLDMKSREITAMAALAALGTAPNQLKIHIKGALNCGCTRDEVVEVLIQVLV
                     YAGFPAALNGIRLAKEVFQEIDGKS"
     gene            2796..3098
                     /locus_tag="VFJ67_10015"
     CDS             2796..3098
                     /locus_tag="VFJ67_10015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HET7290761.1"
                     /translation="MLILIAKFRTKPGCRERMMEMSRGMLGPSRSEEGCIVYEFLQDP
                     FDPDSFTFYEKWKSRHDLDLHFEKPHFKEFEKTFPDLIDGPESVVAYEVSGESVIA"
     gene            3145..>4210
                     /locus_tag="VFJ67_10020"
     CDS             3145..>4210
                     /locus_tag="VFJ67_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7290762.1"
                     /translation="MRGIYVLTILVLLFIAACGNSSKDSGAPRDTLNVNLGNEPPTLD
                     WSLATDSTSINVINNIMEGLTQFSKDFTPEPDLAETWEVSEDGKTYTFKIREGVKWTD
                     GKPLTAGDFEYSWKRILNPETGGDYAYSLYDVENAEEYNTGKITDADKVGVRALDDRT
                     LEVRLKRPASYFPSMLAFITTFPQRKDVIEKHGQEWTNPANIVTLGPYAVTSWRHHDN
                     IVLTENPGYWGEKPRIKKVKMIMNENPSSALAQYEAGELDYADSRSIPPLEVPRLKDL
                     PDFTTTLQFRSNYIAFNVEKPPFDNPLVRKAFAASIDRQSIVGLIQGAGITSKSWIPK
                     NMLGYNQDIGIDFNPEQARKW"
BASE COUNT          977 a         1119 c         1194 g          920 t
ORIGIN      
        1 tcatatatat gcggatgtac ggacgaacgg ggtaaccgcg gaaccttgtg ccttaagacc
       61 cctcgacaca gctaatcccg gctgtcagta gtaattgagg tcgctgtcga tccagtattt
      121 ctcggcgccg acgttcgtgc ggagcgcgat gccttgcttc gtaagctgat aaataatgat
      181 gtcggaatcg aaaggcactt ggccgcttgc tgcggaaccc ttggtctttg acttcagcac
      241 cgcatcgccc tggacgccga aatcccaccc gctgcttata aactgcctca tcaccgcagg
      301 gttattgaag attatcagct ccctgaaatc cgtaagggcc acgccgaggc cgactcctcc
      361 ctgggccatc ctcatgaatg tagttttccc gtttgaatta tcggttatca cgccgtatcc
      421 gttccccgag ccgaggatca ggagctgggt attgatattg ctgaatacgc cgtacccggc
      481 agcatgttgc accaggtcct gcatatacgg gcttttttga taaatctccg cgagagtatt
      541 gtccctcatc tgaagtatgt aagccctttg ggacacgggg gtgttgccct tgggcttggc
      601 gcatccgtaa aacgcaaaag cgataaacag cagggaaaac agaagcaggc ttattctcat
      661 acatatctcc tcatccggaa tttttattaa tcacagacta aaacggattt cctttgttat
      721 ttatattaac ctattttaat cggctcttta acaatctgat gaagtcgccg ttaagttcgg
      781 gctctattat atcacggagt ctggcaaagg cggaaaaaga cacatataat gtgtccgttt
      841 cttgatgcgc atcggacaat aacaatcctg atgcgcgcta ctgccatgaa tcccggaaag
      901 aatagcggaa tcgttatatt cacctcggtg atagccgcgc tgccaggcct tatgctgggc
      961 tacgacacgg gctccgtgag cggcgcgctc ggttttatat cgaacacttt caggctcgac
     1021 accatcggga aagggggcat cgtatcgatg gtgctcatag gcggaataac cggggcgttc
     1081 gtaaacggat tcctggccga caggctcgga agaaagataa ccgtgcaggc agccgctcta
     1141 ctgtttatcg cgggagcggc cggctctgcg ttttcgccga ctgcgggtgc tctccttgcc
     1201 tcccggttcg ttctcggact cgctctgggc gcggtctcgg cgtcggcccc gctgtacata
     1261 gccgagatat cccccgccgc aggcaggggg aggctggtca cttacttcca gctcgcgata
     1321 acgataggca tcctgctcgg ctacatagtg gacagggcgc tcacggggtc gggcgactgg
     1381 aggctgatgc tcgggacagg cgcgataccg gcatttgtac tgctcatcgg aatgttcgtg
     1441 ctcccgccga gcccgaggtg gctcataaag atgggtcgcg aggacgaggc gagggagacg
     1501 ctcatcaaaa tcacaggcgg ggacgcggtt cttgcagatt cggagctgaa cgatataaaa
     1561 aaggttttat ccatggaatc ggacgggggg ttcgcggata tgctgaggcc ccctgtaagc
     1621 cgggcgttcg tcactgccat agggctcttt ctgacacagc aattcgtcgg cataaattcg
     1681 atcatgtact acgcgccttc gctcttcagg ggggccggga tggaatccca gcactctgct
     1741 atactcgcga cggtgagcgt gggcgtagtg aacgtgctcg ccactttcat cgcggtgtgg
     1801 ctcatagaca ggatcgggag gaagcccctc ctctacgccg gtctcgcggg gatgctgata
     1861 tcgctttccg ccataggcct gattttcgcg ctcggggaga gagggggata cgagatcggg
     1921 agggcagcga taatcgccgt ctggttctat atagcgtgct tcgccttttc gctaggtccc
     1981 gtgccctgga tcgtgatgac ggaaatattc ccgctcaacg tgagggggag ggctgtgagc
     2041 atggcgacaa tgtcgagctg gggagcgaac ctcgtggtct cgttttcgtt cctgcctctc
     2101 ctggaagcaa tcggcgcgtc atacacctac tggctctacg ctttcatcag ccttatcggg
     2161 atcgcgttcg tatggcgttt cgttccggag acgaagggga tgacactgga agagatagag
     2221 gaaaggctgc tttccgggaa gaaatgactc tgattaacag gttcggggta agcgggcggg
     2281 ttcaggattt cccgtcaatt tcctgaaaga cctctttcgc gagcctgatg ccgttgagcg
     2341 ccgcggggaa gccggcgtag acgaggacct gaatcagcac ctccacgacc tcatccctgg
     2401 tgcagccgca gttgagcgcg cccttgatat gaatcttcag ctgatttggc gcagtaccga
     2461 gtgccgcgag cgcggccatg gcggttatct cgcgggactt catgtcgagt cccggcctcg
     2521 acgatacctc gccgaagacg aactccatca tgtaccgcac catgtcgggc gcaatgtcgt
     2581 ttagactctc ggcagtcctc ttcgaagcac cgggcgaaac tttctccagt aattccagac
     2641 cgcgtttata tttatcgtcg gacactttat ttctccatta cccttaaata ccgcagcttg
     2701 taactaacat tccgccaccc tctcgcttta cgcttgaaaa tggccccggc caagcctata
     2761 ctacggtttt tcattgtgtt tgtaaagggg gataaatgct catactcatc gcgaaattca
     2821 ggaccaaacc cgggtgccgg gagcggatga tggagatgtc gagagggatg ctcgggcctt
     2881 cgagaagcga ggaggggtgc atagtgtacg agttcctcca ggacccgttc gatcccgatt
     2941 cgttcacgtt ctatgagaag tggaagagca ggcatgacct cgaccttcat ttcgaaaagc
     3001 cccacttcaa ggaattcgaa aaaactttcc ccgacctcat agacgggccc gagagcgtcg
     3061 tcgcgtacga ggtatccggc gagagcgtta tcgcctgact ccgctaagtg gacagccgga
     3121 gacccatact ttaagataag acccatgcgc ggaatatacg tcctgacgat tctcgttctc
     3181 ctgttcatag cagcgtgcgg taacagcagt aaagacagtg gagctccgag ggatacgctc
     3241 aacgtaaacc tcgggaacga acctccgact ctcgactggt cgcttgcgac agacagcacg
     3301 tccataaacg tcataaacaa catcatggag ggactcacgc agttcagcaa agacttcacg
     3361 cctgaaccgg acctcgcaga gacatgggaa gttagcgagg acgggaagac gtacacgttc
     3421 aagatcaggg aaggcgtgaa atggactgac gggaagccgc ttaccgccgg cgatttcgaa
     3481 tattcatgga agaggatact gaacccggaa accgggggag actacgcgta ttccctctac
     3541 gacgtggaga acgccgagga atataacacg gggaagataa cggacgcgga caaggtcggg
     3601 gtcagggcgc tcgacgacag gactctcgaa gtgaggctaa aaagaccggc gtcgtatttc
     3661 ccgagcatgc tcgccttcat cacgacgttt ccccagagaa aggacgtcat tgagaaacac
     3721 gggcaggagt ggaccaatcc ggcgaatata gtcacgctcg gtccttacgc ggtcacttcg
     3781 tggaggcacc acgacaacat agtccttacg gagaaccccg gatattgggg ggagaagccg
     3841 cggatcaaaa aggtgaagat gataatgaac gagaacccgt cctcggctct tgcacagtac
     3901 gaggccggag agctcgatta cgcggacagc aggagcatac ctcctctaga agtgccgagg
     3961 ttaaaagacc tgcccgattt cacgaccact ctgcagttca ggagtaatta catcgcgttt
     4021 aacgtcgaga agccgccgtt cgacaacccg ctggtcagaa aggcgttcgc cgcgtccata
     4081 gacaggcaga gcatagtggg cctgattcag ggggcgggga taacatccaa atcctggatt
     4141 cccaagaaca tgctcgggta taaccaagat ataggaatag acttcaaccc ggagcaggca
     4201 agaaaatggc
//
LOCUS       DASSJH010000456         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1295719, whole genome shotgun sequence.
ACCESSION   DASSJH010000456 DASSJH010000000
VERSION     DASSJH010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295719"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..1625
                     /locus_tag="VFJ67_10025"
     CDS             474..1625
                     /locus_tag="VFJ67_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7290763.1"
                     /translation="MKRIHNFIVLLVFVLATYTQSVAADTTYEGLSNFTRVLDLVERN
                     YVEEVDPEKLTNSAIDGMLKTLDPYSTYLSPERYRELEIGTSGEFGGVGMEVSEENGV
                     LVVITPIEGSPAEKAGIKPRDQIIEIEGKSTQGMVVQEAVRLLRGPSGTPVKITIKRE
                     GEKEPRVVTLIRDKIIVKSVKSRLLDNGIGYVRLVQFQDRTSQELRDAISGLEAQNGR
                     QLNGLILDLRNNPGGLLSEAIDVVDEFIDTGLIVSVRGRTQDQTREYYATKNGTFQTF
                     PVVVMVNDGSASASEVVAEALQDSKRAVILGTKTFGKGSVQTIIKLEDGSGLKLTTAK
                     FYAPSGRSINEVGVTPDIKVENSKDQDKQLESAINILNSSEAGKSVPKG"
     gene            1640..>2014
                     /locus_tag="VFJ67_10030"
     CDS             1640..>2014
                     /locus_tag="VFJ67_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290764.1"
                     /translation="MPKSTRNNGRKRPRRSGKRNGTAYKAFVYGISFVAVLALALYGL
                     YHLKKNIPPQDRSGTVTTERMSALAKEADGNISGAFFDLGVSVKDIKSKKTYKRSSGG
                     INWEFREIIVNTPERVSDKDIRA"
BASE COUNT          564 a          460 c          574 g          416 t
ORIGIN      
        1 ctgtgactat aaccaggaag gtgaggcagt atatgtataa cgatacgggt atgaaaacta
       61 tcttgaaaag gatgaaccaa tccttcgata gctggagcac catggagctg tagctctgta
      121 taaacctgct tagaactgta accaccattt caaacatgca tgtgcctcca gtggataaat
      181 ttatattaat ctcagggact cttcaaatcg ggaaccgata gtcaatactt cgcgcgggag
      241 cttttatgca gacggtataa atacggaacc tgcagcatcc ggcttatttc agccccggaa
      301 gggttaatcg agcaatttcg ttaaatatcg tttaataccg gcatcatgga ataaatcgtg
      361 tttccgcgga ggcggaaccg cgataaggac gtttcctgtc cgagggtcaa atccgggttg
      421 ccgatcaata tatgtttggt taatctctat aaagaggtaa gattatcgtg aaaatgaaga
      481 gaattcataa tttcatcgtt cttttagtat ttgttcttgc tacttacacc caatccgtcg
      541 cagctgatac cacgtacgaa ggtctttcca acttcacgag ggtccttgac ctggtcgaga
      601 ggaactatgt ggaagaggtc gatcccgaaa agctgacgaa cagcgcaatc gacgggatgc
      661 tgaaaacgct cgatccctat tcgacttatc tcagccccga gcggtacagg gagctcgaga
      721 taggcacgtc cggggaattc gggggagtgg ggatggaggt gtcggaggag aacggcgtgc
      781 tggtcgtgat tacgccgata gaggggagcc ccgcggaaaa ggcggggata aaacccagag
      841 accagattat cgagatcgag ggaaaatcca cacaggggat ggttgtgcag gaagctgtca
      901 ggctcttgag ggggccgagc ggcacgcccg tgaagatcac cataaagagg gaaggggaga
      961 aggagcccag ggtcgtaacc cttataaggg acaagataat tgtaaaaagc gtcaaatcca
     1021 ggctccttga taacggaatt ggttacgtca ggctcgtcca gtttcaggac cgtacctccc
     1081 aggagcttag ggacgcgatc tccgggctcg aagcccagaa cggaaggcag ctgaacggat
     1141 tgatactcga cctgaggaac aatccgggcg ggcttctgag cgaggctata gatgtcgtgg
     1201 atgaatttat cgacaccggt ctcatcgtca gcgtccgggg aaggactcag gaccagacca
     1261 gggaatacta cgctacaaag aacgggacct tccagacgtt cccggtagtt gtgatggtga
     1321 acgacggcag cgcgagcgcg tccgaagtcg tggcggaggc tcttcaggac agtaagcgcg
     1381 cggttatact cgggacgaag acattcggga agggctctgt tcagacaatt atcaagctcg
     1441 aagacggatc gggcttgaaa ctcactacgg ctaagttcta cgctccgagc ggcaggtcca
     1501 tcaacgaagt cggcgtgaca ccggatataa aggtcgagaa ttccaaagac caggacaagc
     1561 agctcgaaag cgcgatcaat attctaaact cctcggaagc cggtaaatct gtacccaagg
     1621 gctagggaga gcccgttttt tgccgaaatc gacacggaat aacggaagga agagaccccg
     1681 gagaagcggt aagaggaacg ggactgctta caaggcgttc gtctatggca tttcgttcgt
     1741 agccgtcctt gcgctggccc tttacggtct ctatcatctg aaaaagaata tccccccgca
     1801 ggaccgctcc ggcacggtca cgaccgagag aatgagcgcg ctcgcgaaag aggcggacgg
     1861 aaatatatca ggcgcgtttt tcgacctcgg cgtatcggtc aaggacataa aatccaaaaa
     1921 gacgtataaa agatcgagcg ggggtataaa ctgggaattc agggaaatta tcgtcaacac
     1981 cccggaaaga gtcagcgata aagatataag ggca
//
LOCUS       DASSJH010000457         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1189174, whole genome shotgun sequence.
ACCESSION   DASSJH010000457 DASSJH010000000
VERSION     DASSJH010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189174"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFJ67_10035"
     CDS             <1..336
                     /locus_tag="VFJ67_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290765.1"
                     /translation="ITFGKGEEQIFLGKELSRPKDYSEETAVEIDNEIRRIVSENYTK
                     AKAVLEENIDLLHKLTEVLLDKEVIDGKELDELVKEFKSKDFPTPWERPIGSEGSVAL
                     RNSSSAKDT"
     gene            469..1401
                     /gene="folP"
                     /locus_tag="VFJ67_10040"
     CDS             469..1401
                     /gene="folP"
                     /locus_tag="VFJ67_10040"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7290766.1"
                     /translation="MQQKEADAYVEPEPLHFNGSTPVIKKHTFKCRDKTLRLHEKTHI
                     MGILNLTPDSFHDGGRHADTDSALRHADAMIEGGADIIDIGGESTRPGSGGVTEEEEL
                     LRVMPVLRELVKRFDTVLSIDTTKERVAREALAEGVSIVNDISGLTYSRGIAEAASEY
                     GAGLVVMHTPSRPPDMQEKTQYVSLMDDIAGALRASVDKALDAGVSGESIMIDPGFGF
                     GKTAEQNVIILRDLMTLSELGMPVMIGTSNKSFIGKIAGGETEDRLLGTAATVAIGIM
                     NGASVVRVHDVARMRSVVRMADAVAGKNYTGTER"
     gene            1405..2235
                     /gene="cdaA"
                     /locus_tag="VFJ67_10045"
     CDS             1405..2235
                     /gene="cdaA"
                     /locus_tag="VFJ67_10045"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET7290767.1"
                     /translation="MFDAILNFRYVWDTLDIILVAFIIYYVLRLLEGTRALQVLLGFV
                     LLIVLYYVSQRGLFTLNWILGQFLGSIIIIVVILFQNDIRRGLAAIGRRPFLLKMSPG
                     KSKELFTEEIVKAASYMANRHIGALMAIERVNSLSDFVEIGMRIDALVSRELIISIFN
                     PSSPLHDGGIIIVGNRILSAGSFFPLATDPDLERDLGTRHRAAIGLSLETDAIVIVVS
                     EETGIVSLAVGGEIKRGLDATSLQNQLLDLLGIKRSARNKADTAGGIIEKEEGEKVNT
                     "
     gene            2245..>3087
                     /locus_tag="VFJ67_10050"
     CDS             2245..>3087
                     /locus_tag="VFJ67_10050"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HET7290768.1"
                     /translation="MSDWSKGILGSKGNRGRDQEPLIRDAGKKILAILIAITLWFVAN
                     LQHDVERNIMIDVNYTNLPKGLIIVNNPPEKLNIRVRGPRSQLSSLSSKDMVFTIDLS
                     NLSTGTSMFEIRTDQLIPPRDVQVTGISPSEINLDVDKVAQKRVSVEPVLEPPDNGYE
                     IAAKPQVTPETVVIRGPEKLLRTIKSVGTDPVSLKGEKSRFSIEVPIRPPYSLVEIEG
                     GNTVKVTIDIKEKIMEKEFNNLDINFINFDGMKFETDGSISAEIAFEGPFSILNNLNS
                     KDIEL"
BASE COUNT          835 a          769 c          898 g          585 t
ORIGIN      
        1 attacgttcg gcaaggggga ggagcagata ttcctcggca aggagctatc caggccgaag
       61 gactacagcg aagagacagc cgtcgagatc gacaacgaaa taagaaggat cgtgagcgag
      121 aattacacaa aggcaaaagc ggtcctcgaa gagaacatcg acctgctcca taaactgacc
      181 gaggtcttgc tcgataaaga ggtcatagac gggaaggagc tcgacgagct tgtaaaggaa
      241 ttcaaatcga aagattttcc gaccccctgg gaaagaccta taggctccga aggctcggta
      301 gccttaagga acagcagcag cgccaaagac acatgaattc ggacagccgc cttccgtcgg
      361 tttttgcagc atagatagaa agtataatta taaaaataca ctttccgcgc gggggctttc
      421 gatcggagcc tcatgctatg aaaaatagac agcggaaaaa ggattgctat gcagcagaaa
      481 gaagctgacg cttatgtcga acccgaaccc ctacacttta acggaagtac acccgtaata
      541 aagaaacata catttaaatg cagagacaaa acactcaggc tccatgaaaa gacccacatc
      601 atgggcattc tgaacctcac cccggattcg ttccatgacg gggggaggca tgcggatacg
      661 gattcggccc tcaggcatgc agatgcaatg atcgagggag gagccgacat aatagacata
      721 gggggggagt ccacgaggcc cggctcaggg ggcgttaccg aggaagagga gctcctgagg
      781 gtgatgcccg tgctcaggga attggtgaag aggttcgaca ccgtcctctc gatcgacacg
      841 acgaaggaaa gggtcgcgcg cgaagccctg gcagagggag tttcaatcgt gaatgacata
      901 agcggtctta cttactcgcg cgggatcgcc gaggcagcgt ccgaatacgg ggcgggcctc
      961 gtcgtgatgc acacgccgtc ccgtcccccg gacatgcagg agaagaccca atacgtatct
     1021 ctcatggacg acatagccgg cgcgctgaga gcatcagtag ataaggccct cgatgcgggc
     1081 gtgagcgggg agagcatcat gatcgacccg gggttcggct tcggaaagac cgccgagcag
     1141 aacgtcatca ttctgagaga cctcatgact cttagcgagc ttggtatgcc cgtaatgatc
     1201 gggacgtcca ataaatcgtt catagggaaa atagcgggcg gggagacgga ggacaggctc
     1261 ctcggcaccg ccgccacagt ggctatcggg ataatgaacg gggcctccgt cgtaagggtg
     1321 cacgatgtcg cccggatgag gagcgtggtc aggatggctg acgcggtggc cggtaaaaat
     1381 tatacgggga cggaaaggta ggaaatgttc gacgcaatac tgaacttcag atacgtgtgg
     1441 gacacgctcg acataatact cgtcgcgttc atcatatact atgtcttgag actcctcgaa
     1501 ggcacgcgcg cgctccaagt gctgctgggc ttcgtactgc tcatcgtgct ctactacgta
     1561 tcgcagaggg gtctcttcac actcaactgg atactggggc agttcctggg ctcgataatc
     1621 attatcgtag taatactatt ccagaacgat atcaggagag ggctcgctgc cataggcagg
     1681 aggccttttc ttctgaaaat gtcgcccggg aaatcgaagg agctatttac ggaggagatc
     1741 gtaaaggccg caagctacat ggccaaccgt cacatagggg cactgatggc gatagaaagg
     1801 gttaacagcc tttccgactt cgtcgagata ggcatgagga tagacgcgct cgtgtcgagg
     1861 gagctgatta taagcatatt caacccctcc tcccctcttc acgacggcgg gataatcatc
     1921 gtcgggaaca ggatactgtc tgcggggtcg ttcttcccgc tcgcgaccga ccccgacctc
     1981 gagcgcgatc tcggcacgcg gcacagggcg gcgatcgggt tgtctctcga aacggacgcc
     2041 atcgtgatag tcgtctccga agagacgggt attgtttcac tcgcggtggg gggagagata
     2101 aaaaggggcc tcgacgccac gtcgctccag aaccagctgc tcgatctgct gggtattaag
     2161 agatccgcca ggaataaggc ggatactgcc ggcggaataa tcgaaaagga ggaaggtgag
     2221 aaggtcaaca cctgagaggg cccgatgtca gactggtcaa aaggaatact cggctcgaaa
     2281 gggaacaggg ggagggatca ggaaccgctc atcagggacg cgggtaagaa gatcctcgct
     2341 atactcatcg ccataacgct gtggttcgtt gctaacctcc agcacgacgt cgagaggaac
     2401 atcatgatcg acgtgaacta tacgaacctg ccgaaggggc tcatcatagt taacaacccg
     2461 cccgaaaagc tcaacatacg cgttaggggt ccgcggagcc agctctcttc cttatcttcc
     2521 aaggatatgg tgtttacgat cgacctgtct aacctctcga cgggcacctc gatgttcgag
     2581 ataaggaccg accagctgat accgcccagg gacgttcagg ttactggtat cagcccttcg
     2641 gagatcaacc tcgacgtcga caaggttgcc cagaaaaggg tgagcgtgga gccggtactg
     2701 gagccgccgg acaatgggta tgaaatcgct gcaaagccgc aggtgacgcc cgaaaccgtc
     2761 gtcataaggg ggcccgagaa gctgctgaga acgataaaga gtgtggggac ggaccccgtc
     2821 tctctaaaag gggagaaatc cagattcagt atcgaggtcc ccatacgccc gccctactct
     2881 ctcgtggaga tagagggcgg caacacggtt aaagtaacta tcgatattaa agaaaagata
     2941 atggaaaagg aattcaacaa cctcgacata aatttcatca acttcgacgg aatgaagttc
     3001 gagacagacg gcagcatatc ggccgagatc gctttcgagg gacctttcag tatccttaac
     3061 aatcttaaca gtaaggatat agaatta
//
LOCUS       DASSJH010000458         5011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_1189686, whole genome shotgun sequence.
ACCESSION   DASSJH010000458 DASSJH010000000
VERSION     DASSJH010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189686"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFJ67_10055"
     CDS             complement(<1..887)
                     /locus_tag="VFJ67_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7290769.1"
                     /translation="MKKPTIFAIDDDPAVIRAIERDLRKYYRKDYRVMSANSGEDALD
                     SLRKLKTRNESVALFVTDQRMPGMSGVDFLALAKDIYPDAKRALLTAYSDTDSAIRAI
                     NEAQLDYYLMKPWDPPEERLYPVLNDLLEEWLASYKPDYDGLKLVGYQWSPHSHAIKE
                     FLAGNLVPYLWLDVELNEKGRELISLSGIDHKDLPAAFFEDGTHLTNPDFRALARKLG
                     LKSEASRDVYDVVIIGAGPAGLAAAVYGASEGLKTLLVEKRAPGGQAGTSAKIENYLG
                     FPSGLSGSELTRRAITQAT"
     gene            complement(925..2325)
                     /locus_tag="VFJ67_10060"
     CDS             complement(925..2325)
                     /locus_tag="VFJ67_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7290770.1"
                     /translation="MIIKELREIDLFSDTSDEELEEDVRGVLIELKKGDHLFHEGDNA
                     VNFYIFLEGTLEIYRIIRGEKLSIGFFTKGMSGGEVPLLSGTRHLANGIAVTDMKVFK
                     VDQDDFWYMLGNCSTARGKILSNMSTRISALTHLSFQREKLASLGTMAAGLAHELNNP
                     ASAAKRTAESLASTIHEFDIFSTEILKHYMFRDEVDKAGFPFKTIEDVIDVNGVEMDA
                     LDRNDLEDELAEWMEEEGVEDPSELASILVSAGFTKRILSDFSVNLKPDQVVNFLNWL
                     PRDVEMRKLAGELVESTSRISELVSAMKSYTYMDKTHEKRKTDLHEGIENTVIILNHK
                     LKKKSITVKKEFDYNIPKISAYGVELNQVWTNLLDNAIDAVPDRGVITIRTGFNDNGS
                     NTVNVEIVDNGGGIPPEVQDKIFDPFFTTKAPGEGSGLGLEISYRIIVNQHKGSIGFE
                     SRPGFTKFSVCLPVDL"
     gene            complement(2530..4641)
                     /locus_tag="VFJ67_10065"
     CDS             complement(2530..4641)
                     /locus_tag="VFJ67_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290771.1"
                     /translation="MMSDIRSRTVNYRYLVTILLSVVFVNAFTVYYMKQERYIYNWDM
                     LAYWDPYIEIGRSLLISPLGTIKNILLTVRYSDYNYLPVVPLLPFYYIFGETRLGYIL
                     AISNIYMLPSMLILLYLSDRVISAYRSNVDPLVFWLVFITILISMPLWIPTLRGYPDI
                     LGTILIGLILLIYLGTPLEKQNFILLLLTAGFLAIMSISRRYFNFWVEGFFVAAFLER
                     SLNILLSGTRNYRAYLNLLINLFIIAIIFLVLDVLITGPMIKRQLTTHWSEIYGAYQE
                     QSSWLGRCLEFPEHFGPLYSALVVIGMVLSVWTRRIRQFAVFLIIQTIVTVAAFTKVQ
                     SFDYHQYLMLCPGFLLFMAVFIDRSASSFQSLIPRIIFTVLYLIFIVTMFAYSLVPGF
                     TMPKSVLFLYPKEQEEYKPQVRNDLEEINRLLEYIEDLAAEKPGSTFYLLSSSPLWNS
                     SILQTAVKGKYKDPDTIPAINTTSDVDKRDGFPMKFFTSNYVIVAEPFQYHLDKNNQR
                     IITIPAEKILKREGIGLNYRELPERFSLDKNIKLRIYERVKPPVYAAIRSTLNDFLAY
                     YPEMAERYDVLPLELFVSGGDGAGGNVNVVFTPDNRVALKLDGDRPTEAVFSFGGDLK
                     RMKITPSLGENCDSPAMVEIEMTGDGREIFRHAVGRDNAESYDVDLTGVDLLKLSAVS
                     TGQQPCDSLNIEVVSQDWKTK"
BASE COUNT         1230 a         1304 c         1180 g         1297 t
ORIGIN      
        1 ctcgtcgcct gagtgatcgc acggcgcgtg agctcggagc cgctcaggcc gctcgggaag
       61 ccgagataat tctcgatctt cgcgctcgtg cccgcctgac ctcccggggc tctcttctcg
      121 acgaggaggg tcttgagacc ctcggacgcc ccgtacacgg cggcggcaag ccccgccggc
      181 cccgctccga ttatcacgac atcgtaaaca tcccgcgagg cctccgactt aagacccagc
      241 ttacgcgcaa gcgccctgaa gtcgggattc gtcaaatgag ttccgtcctc gaaaaaagcc
      301 gcaggcaggt ccttatgatc aatccccgac aagcttataa gctccctccc cttctcattc
      361 agctccacgt cgagccagag atacggcacc aggttcccgg ccaggaattc ctttatcgcg
      421 tgcgaatggg gcgaccattg gtaaccgacc agtttcaaac cgtcgtagtc cggcttgtac
      481 gacgccagcc attcttcgag caggtcgttc aggactggat agagtctttc ctcgggaggg
      541 tcccacggct tcatcaggta ataatccagc tgcgcctcgt tgattgccct tattgccgaa
      601 tccgtgtcgg aataagccgt aagcagcgcc ctcttcgcgt ccgggtaaat gtccttcgca
      661 agggcgagga aatccacccc cgacatcccg ggcattctct gatccgtaac gaaaagcgcg
      721 accgattcgt tcctggtctt tagtttacgg agactatcga gggcgtcttc tcccgaattt
      781 gcggacatga ctcggtaatc ctttctgtag tacttccgga ggtccctctc gatggccctg
      841 attacggcgg ggtcatcgtc tattgcgaat atcgtaggtt ttttcatgat ccaagattcc
      901 ctgtatggtc ccaatgtcac gcggtcacaa atccaccggc aggcagacgg agaattttgt
      961 aaatcccggc ctcgattcga accctatact ccccttatgc tgattgacta ttatgcggta
     1021 actgatctcg agtccgagtc ccgaaccctc acctggcgcc ttggtcgtaa agaacgggtc
     1081 aaaaatctta tcctgcacct cgggcgggat tcccccgccg ttatcgacaa tctccacatt
     1141 caccgtattc gagccgttat cattaaaccc ggtccttata gtaatcacgc ccctgtcggg
     1201 cacggcgtcg atcgcgttgt cgagcaggtt agtccacacc tggtttagct caaccccgta
     1261 ggcggatatc ttcggaatgt tataatcgaa ctcttttttc actgtaatgc tctttttctt
     1321 aagcttatga ttgaggatga tgacggtatt ctctatgcct tcatgtaaat cggtcttcct
     1381 cttctcgtgt gttttatcca tgtaggtata agatttcatt gccgacacaa gctccgatat
     1441 cctcgacgtg ctttctacga gctccccggc gagcttccgc atctcgacgt ccctcgggag
     1501 ccagttcagg aaattcacga cctggtccgg tttcaaattc acggaaaaat cgctcagtat
     1561 ccgcttggtg aaaccggcgg ataccaggat ggacgcaagc tccgagggat cttccacacc
     1621 ctcctcctcc atccactccg cgagctcgtc ctcgagatcg ttcctgtcga gcgcgtccat
     1681 ctcgacgccg tttacgtcaa taacgtcttc tatcgtttta aaggggaagc cggccttatc
     1741 gacctcgtcc ctgaacatgt aatgtttcag tatctccgtc gagaagatat cgaactcgtg
     1801 gatagtgctg gccaggctct ccgccgtcct cttggccgcc gaagccggat tattgagctc
     1861 atgggcgagg cccgccgcca tcgtaccgag agaagccagc ttctcccttt gaaatgagag
     1921 gtgcgtaaga gccgagattc tcgtcgacat attggataat atttttcccc ttgcagtcga
     1981 gcagttgccc agcatgtacc agaaatcatc ctgatcgact ttgaagactt tcatgtcggt
     2041 aacggctatt ccgttcgcaa gatgcctggt gccggaaaga agcggcacct ctccgccgga
     2101 catgcctttg gtgaagaagc ctatgctgag cttttctccc ctgattatcc tgtagatttc
     2161 gagcgtgccc tcgaggaata tgtagaaatt aacggcgtta tcaccttcgt gaaaaagatg
     2221 gtcgcctttc ttcaattcga tcagtacgcc cctgacgtcc tcttccagct cttcgtccga
     2281 agtgtcggaa aaaaggtcta tttccctcag ctcttttatg atcatcggcc ttagctcatt
     2341 gaaactcgaa tgacatttat tgtaaacggt ggtttggatt ccggcaaaat aaagctgggt
     2401 gtaattaata aactcggcgg aatttaaatt aaaagaaagc taagagttag cgcgacatcc
     2461 gatgaattat agtaaataat tctctttgtt tccgtagaga catggaatcg agattctcag
     2521 gcatctacgt cattttgttt tccagtcctg agatactact tctatattta aagaatcgca
     2581 gggttgctgg cctgtactga ccgccgagag tttcagaaga tcgaccccag tcaaatcgac
     2641 atcgtagctt tccgcattat cccgcccgac agcgtgcctg aaaatttccc tgccgtctcc
     2701 cgtcatctca atctcgacca tggccgggct atcgcagttt tcaccgagcg acggcgtaat
     2761 cttcattctc ttcaaatccc cgccgaagct gaagacggct tccgtcggcc ggtcgccgtc
     2821 caatttaagt gccaccctat tatccggtgt aaacaccacg tttacatttc cgcccgcgcc
     2881 gtctcctccg gagacaaaga gctcgagggg tagaacgtcg tatctctccg ccatctccgg
     2941 atagtaggcc aggaaatcat tcagggtgct ccttatggcg gcgtaaacag gcggcttcac
     3001 tctctcgtat atgcggagct taatgttctt gtcgaggctg aaacgctcag ggagctccct
     3061 gtagtttaat ccgatccctt ctctcttgag aattttttct gcgggtatcg ttataatccg
     3121 ctggttattt ttatcgagat ggtattgaaa cggctcggcc actattacat agtttgaagt
     3181 gaagaacttc atcgggaacc cgtccctctt gtccacgtcc gaggtagtat tgatcgcggg
     3241 tatcgtatcc gggtccttgt attttccctt tacggctgtt tgcaggatgc tcgaattcca
     3301 gagcggagag ctggataata agtagaaggt tgaacccggc ttctcagcgg ccaggtcttc
     3361 gatgtattca agaagcctgt taatttcttc aagatcgttc ctgacctgcg gtttatactc
     3421 ttcctgttcc ttggggtaga ggaacaatac cgacttgggc atggtaaaac cagggaccag
     3481 cgaataagcg aacatcgtaa cgatgaatat cagatagagg acggtgaaga ttatcctcgg
     3541 gattaaactt tgaaacgacg acgccgacct gtctataaat accgccatga acaacagaaa
     3601 gccgggacat aacatcagat actgatggta atcgaaagac tggacctttg tgaaggccgc
     3661 tacggttacg attgtttgaa tgatcagaaa aacagcaaat tgccttatgc gccttgtcca
     3721 tacggataaa accatcccga ttacaacaag ggccgaatag agcgggccga aatgctccgg
     3781 gaattccaga cacctgccga gccatgaaga ttgctcctgg tatgctccgt atatttccga
     3841 ccagtgcgtt gtgagctgcc ttttaatcat cggtcctgta atcaggacat cgaggaccaa
     3901 aaagattatt gcgattataa agaggtttat taacagattg agatacgctc tgtaattcct
     3961 cgtgccgctc aagagaatgt tcaatgatcg ctcgagaaaa gccgcgacaa agaacccttc
     4021 cacccagaaa ttgaaatagc gtctgcttat gctcatgatt gcaagaaatc cggcagtgag
     4081 cagcagcagt atgaagtttt gcttctctaa cggtgtccca agatagatta gcaatataag
     4141 tccgatgaga atagttccca ggatgtccgg gtaaccgcgg agagtaggta tccacaatgg
     4201 catggatatg aggatggtta tgaacacgag ccaaaaaact aacggatcga cattactacg
     4261 gtatgcgctg attaccctgt ccgaaaggta taatagaatg agcatggacg gcagcatata
     4321 aatattggat atagccagta tatatccgag tctcgtttcg ccgaatatgt aataaaacgg
     4381 aaggagcggt actacgggaa ggtagttata atcgctatac ctcaccgtta ataatatatt
     4441 tttgatggtc cccaaaggcg agataagcag gctgcggccg atctcgatat aagggtccca
     4501 atacgcgagc atatcccagt tgtagatata tctttcctgt ttcatgtaat agaccgtaaa
     4561 agcgttcacg aatacgaccg aaagaagtat cgtgaccagg taccgataat tgacggttct
     4621 tgaccgtatg tcactcatca taggtcatac ccttactata tattcattcc cggcctgata
     4681 ataccgccat gatggtgaac aattaccgac gtttcggaat gtttttttca catacccctc
     4741 cggcaacggg gcgcccttct ctaaaagatc cggaatccag gggccgttaa atgtacagga
     4801 tatattatac tcctggccat aataaacagg gaatatgaga ctgtcataaa cggaatcaaa
     4861 tctcggaagg accgtgacct gatacagatg atgatttcta ttagtttata gaggaagatt
     4921 aactttcccc tttgttgaac tgccgcgagc ggacaggtac attaatgact ccttagtttc
     4981 cgaaaacaac tggaaatagg gaatataaag a
//
LOCUS       DASSJH010000459         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_971459, whole genome shotgun sequence.
ACCESSION   DASSJH010000459 DASSJH010000000
VERSION     DASSJH010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971459"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFJ67_10070"
     CDS             <1..260
                     /locus_tag="VFJ67_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7290772.1"
                     /translation="LSAFYKRVRPWGWWNPVSELNPGIDKPPFSPVLINWVLGVSCIL
                     FGMIGVGKILLGSFIPGVLMIAVSLVSGITVYSRLKKELAD"
     gene            342..938
                     /locus_tag="VFJ67_10075"
     CDS             342..938
                     /locus_tag="VFJ67_10075"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET7290773.1"
                     /translation="MAINVKVLKHPELTWYEKLYLPQILGGLLITLKHLILFKPITIQ
                     YPEEVKELPPRYRGIHVMPADDDGEIRCVACKLCEVACPTQAISIVAEPADDYGIERR
                     PKVYNIDFMRCVFCGFCVEACPCDALRMGMKYELASYNRAGLVHTKEVFFDPNVKSHA
                     PRDNANFLYEMGRGEILDKPEEVDNVKKLTGTYTSARS"
     gene            complement(962..1981)
                     /locus_tag="VFJ67_10080"
     CDS             complement(962..1981)
                     /locus_tag="VFJ67_10080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7290774.1"
                     /translation="MNNTDPYISVIVPVHNGSGYIEECLGALQRSLCGSFETILVDDA
                     STDDSREICEGFGVKVVALERQSGPGGARNEGAKHAAGEILLFIDSDVVVTENTLGQF
                     ADIFKNDPDISAVFGSYDDSPRADDFISQYRNLLHHFVHQRSGTEANTFWAGCGAVRR
                     SVFTELGGFDAERFPLPSIEDIELGYRMRQKGYGIMLAKDIQVKHLKHWEWLSVLRTD
                     IFNRAVPWSKLILETKTMPRDMNLRMTDRVSTALTGLLFISVLILVLELIGVLRMVPF
                     ARIAFLALIISAALLILNRDVYGFFIRKRGLGFTLLAIPMHLFYYLYSGVSFAVCWIK
                     KKIWN"
     gene            complement(2039..>2922)
                     /locus_tag="VFJ67_10085"
     CDS             complement(2039..>2922)
                     /locus_tag="VFJ67_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7290775.1"
                     /translation="ILARILPVIKQINQSRGIIVSRIPALDAVYDLVRLDNKPVLKDG
                     DKPYAGLKSGIRFESVTFSYDDNELAALTEADFFIPKGKTTAIVGKSGAGKSTIINLL
                     LRFYDPQAGRILIDEEPLGSFRVETYRRSIGIVSQDTFIFNDTVRSNIAFGITGERSD
                     EIITDAAKRAGADEFISRLSQGYDTILGERGVRLSGGQRQRISIARAILKNPEILVLD
                     EATSSLDTRTEELIHNAITDLSRNRTVVIIAHRLSTIKNADQLIVLKDGRVSETGSEP
                     ELMELKGEYYKLATSGK"
BASE COUNT          625 a          821 c          758 g          718 t
ORIGIN      
        1 ccctctcggc gttttacaaa agagtgcgtc cctgggggtg gtggaatccc gtgagcgagc
       61 tcaatcccgg catcgacaag cccccgtttt ccccggtcct gataaattgg gtcctcggcg
      121 taagctgtat cctgttcggg atgataggtg tcggaaaaat ccttctcggt tcattcattc
      181 ccggcgtact gatgatagcc gtctcactcg tctccggtat caccgtttat tcgaggctta
      241 agaaagagct tgcggactga tcgtgcccgt atcgttgcgc ggctttaaaa atcccccgtt
      301 tatgttataa gttatatgca gatacggacg ggaatagaaa tatggcaatt aatgtaaaag
      361 tgcttaaaca tccggaactt acgtggtatg agaagctcta cctgccgcaa attctcgggg
      421 ggcttttaat cacgctcaag catttgatcc tttttaagcc catcaccata cagtatccgg
      481 aagaggtaaa agagctcccg cccaggtaca ggggtatcca cgtgatgccc gccgatgacg
      541 acggcgagat aaggtgtgtc gcgtgcaagc tctgcgaagt ggcgtgcccc acgcaggcga
      601 tttcgatagt agccgagccc gccgacgatt acggcataga gcggagaccg aaggtctaca
      661 acattgactt catgaggtgc gtattctgcg gtttctgcgt cgaggcgtgt ccttgcgacg
      721 ccctcaggat gggcatgaag tacgagctcg cctcttacaa cagggcgggg cttgtccaca
      781 cgaaggaagt atttttcgat cccaacgtga agagccatgc tcccagagac aacgccaatt
      841 tcctttacga gatgggcagg ggagagatcc tcgacaagcc cgaagaagtg gacaatgtaa
      901 agaaactcac cgggacatat acctccgccc ggtcctgagt tttatctatc ccagcacaag
      961 cttaattcca gatcttcttt tttatccagc atacggcgaa cgaaacgccc gagtatagat
     1021 aatagaacag gtgcatcggt atcgcgagca gggtgaatcc gagccctctt ttccgtataa
     1081 aaaatccgta aacgtccctg ttgaggatta acagcgcggc cgagattatc agagcgagga
     1141 aggctatcct cgcaaacggg accatgcgca aaacgccgat gagctctaac accagtatga
     1201 gcacgcttat gaaaaggagc cctgtaaggg ccgtgctcac cctgtccgtc attcggaggt
     1261 tcatatccct cggcatcgtt ttcgtctcga gtataagctt cgaccacggc acggcccggt
     1321 tgaaaatatc ggtcctgagc accgacagcc actcccagtg tttcaggtgt ttcacctgaa
     1381 tatccttggc gagcattatg ccgtagccct tctgccgcat cctgtaaccg agctctatat
     1441 cctctatcga cgggagcggg aacctctcgg catcgaagcc gccgagctcc gtgaatacgc
     1501 tgcgccttac tgccccgcac cccgcccaga aggtgttagc ctccgtaccc gacctctggt
     1561 gcacgaagtg gtggagcagg ttcctgtact gagatataaa atcatcggcc cttgggctgt
     1621 cgtcgtacga tccgaagacg gccgatatat ccgggtcgtt tttgaaaatg tccgcgaact
     1681 gtccgagcgt gttctccgtc acgacgacgt ctgagtcaat gaaaaggagt atttccccgg
     1741 cagcgtgctt tgccccctcg ttccgcgccc ctccgggccc tgattgtctt tcgagcgcga
     1801 cgactttgac cccgaaacct tcgcatatct cacgcgagtc gtctgtggat gcgtcgtcaa
     1861 ccaatatcgt ttcaaaagac ccgcacagcg acctttggag cgctccgaga cattcctcta
     1921 tataaccgct cccgttgtgt acggggacga ttacagatat ataagggtcc gtgttattca
     1981 tatagttctg attcacggtg actacggatt ccggacaggg cggcgtatta ttcccgaatt
     2041 acttgcccga tgttgccagt ttatagtatt cccctttgag ctccataagc tcaggctctg
     2101 agcctgtttc cgagaccctt ccgtccttga gcacgataag ctggtccgcg ttcttgatag
     2161 tcgaaagcct gtgggcgatt atcacgacgg tcctgttcct gctgaggtcg gtaatggcgt
     2221 tgtgaatgag ctcctccgtc ctggtatcga gggagctcgt cgcctcgtcg aggaccagta
     2281 tctccgggtt tttcagtatc gcccgcgcga tggatatcct ctgcctctga ccgcccgaga
     2341 gcctgacgcc cctttctccg agaatcgtat cgtagccctg cgacagccgg gatatgaatt
     2401 cgtcggctcc cgccctcttg gccgcgtcgg tgattatctc gtccgaccgc tcgcccgtga
     2461 tgccgaaagc tatgttgctc ctgaccgtat cgttgaatat gaacgtatcc tggctgacta
     2521 tcccgatgct cctgcggtaa gtttctactc tgaagctccc gagcggctct tcgtcgatta
     2581 aaattcggcc tgcctgcggg tcataaaacc tcaaaagaag gtttattatc gtggatttgc
     2641 ccgctcccga tttgcctacg atagcagtgg tcttgccctt cggtataaaa aaatccgctt
     2701 cggttagagc cgccagctcg ttatcgtcgt aggagaaggt tacggactcg aaccttatcc
     2761 cgctcttgag ccccgcatac ggcttgtccc cgtccttcag cacgggctta ttgtcgagcc
     2821 tgacgaggtc gtacaccgcg tcgagcgcgg ggatcctgct tactatgatc cccctcgact
     2881 ggtttatctg ctttatcacc gggaggatgc gggcgagtat gt
//
LOCUS       DASSJH010000460         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U1110
            k141_648644, whole genome shotgun sequence.
ACCESSION   DASSJH010000460 DASSJH010000000
VERSION     DASSJH010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963271
            Sequence Read Archive: SRR25158195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:40:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,020
            CDSs (total)                      :: 1,996
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648644"
                     /isolate="SMAG_U1110"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.32277778 N 120.3511111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFJ67_10090"
     CDS             <1..651
                     /locus_tag="VFJ67_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7290776.1"
                     /translation="VDAGSILLVELDGPGAEVEAQMPVVEDVLRENHAHEIRIARDAR
                     ERELFWKGRKNAFPAMGRVSADYYVQDGVIPRSKLAEVLGRIGELSREYGLSVGNVFH
                     AGDGNLHPLVLYNSKIDGQHEKALELAGKILSVCVEAGGSITGEHGVGNDKKRFLPLM
                     FDENDLDTMNLVRCAFDEKGLSNPGKVFPTPRTCVEQGMKPYREHPLQKAGLGEMY"
     gene            711..2042
                     /locus_tag="VFJ67_10095"
     CDS             711..2042
                     /locus_tag="VFJ67_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7290777.1"
                     /translation="MPESSLKNELTGIVGGENAVDDPRLLEPFTVEGIKPGIALFPKN
                     IEQISEIMKAASRAKACVIPMGSGTKRALGNRPSGVDIVLSTRKLNRVVEHESADLVA
                     TTECGIALSELNAGLGVKNQFLPVDPPHIAAGATVGGIIATNDSGPLRLRYGTARELL
                     IGMKVVRPDGSVFKGGSKVVKNVAGYDLPKLFVGSLGTLGIIAEATFRLYPVPESSRT
                     YFAAFESLDEAHDTVRSLIDSNLVINALEHMNPALAGGLAGSYGPGQDISRYSIAVRI
                     MNVSRAVDDQMKTVVGITQGNGGNGFMIEGEKEEAFWKDVREFPWMPLDSPYAVLKAG
                     LLISEVPRIFLALERLADKFGIRARASARAANGVVIISVAGANETVLTAVKELREFSE
                     SAGGSLVVSEASPDIKPGIDVWGTIGSSLNMMKRIKSGFDPGNILNPGRLF"
     gene            2137..>2356
                     /locus_tag="VFJ67_10100"
     CDS             2137..>2356
                     /locus_tag="VFJ67_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HET7290778.1"
                     /translation="MIRRDDTDGWILITQYDHAVLSGRIMDRWGNETFSSPDPGEETL
                     FAVREHDCGWEDWDSSPKIYPENGYPANF"
BASE COUNT          539 a          650 c          718 g          449 t
ORIGIN      
        1 gttgacgcgg gctccattct cctcgtagag ctcgacgggc ccggagccga ggtcgaggcc
       61 cagatgccgg tcgtcgaaga tgtgctcagg gaaaatcacg cgcacgaaat aagaatagcg
      121 agagatgccc gggagaggga gctcttctgg aaggggagga agaacgcttt tcccgctatg
      181 ggcagggtga gcgccgatta ttacgtacag gacggcgtaa taccgaggag caagctcgca
      241 gaggtgctgg ggaggatagg ggagctgagc agggaatacg gcctgagcgt cggaaacgtt
      301 tttcacgcgg gcgacggaaa cctgcacccg ctcgtccttt ataactccaa aatagatggc
      361 cagcacgaaa aggctctcga gctcgcggga aagatactct cggtgtgcgt ggaggcgggc
      421 gggagcatca cgggggagca cggggtcggg aacgacaaga aaaggttcct cccgctcatg
      481 ttcgatgaga acgatctcga cacgatgaac ctcgtcaggt gcgcgtttga cgagaagggg
      541 ttatccaatc cgggcaaggt gttcccgact ccgaggacgt gcgtcgagca gggaatgaag
      601 ccttatcgcg agcacccgct tcagaaggcc ggcctgggcg agatgtactg atccccgctg
      661 cggcttccgg attcgcacgt ataaaatttt cggacggtaa aaaattataa atgcctgaat
      721 cttccttaaa aaatgaattg accggcatcg tcggcggaga gaacgcggtc gatgaccccc
      781 ggctcctcga accgttcacc gtcgaaggca tcaaacccgg gatcgcactc ttccccaaaa
      841 acatagagca aatatcggaa ataatgaagg cggcgtcccg cgccaaggca tgcgtgatac
      901 ccatgggctc gggaacgaag cgggcgctcg gaaacaggcc ctcgggtgtc gatatagtgc
      961 tctcgacgag gaagctcaac cgtgttgtcg agcacgaatc cgcagacctc gtggccacga
     1021 ctgaatgcgg cattgcgcta agcgagctca acgccggtct cggcgtaaag aaccagttcc
     1081 ttccggtcga tcctccgcac atcgccgcgg gcgcgacagt cggcggcatt atcgcaacta
     1141 acgacagcgg gcctctcagg ctaaggtacg ggaccgcgag ggaactcctc atagggatga
     1201 aggtcgtgcg cccggacggg agcgtcttta aaggcgggtc gaaggtcgtg aagaacgtcg
     1261 ccggatacga tctgccgaag ctcttcgtcg gctcgctcgg gacccttgga ataattgcag
     1321 aagcgacttt caggctctac cccgttcccg agtcttcccg cacttacttc gcggctttcg
     1381 aatcactcga cgaggctcat gacacggtaa ggtctctcat cgactctaac ctcgtaataa
     1441 acgcgctcga gcacatgaac cccgcgcttg ccggaggatt ggccgggagc tacgggcccg
     1501 gtcaggacat cagccgctac tcaatcgccg tcaggatcat gaacgtgtcc cgtgccgtgg
     1561 acgaccagat gaaaacggtt gtcggcatta cgcaggggaa cggaggcaac ggctttatga
     1621 tagagggtga aaaggaagag gccttctgga aagacgtgag ggaattcccg tggatgcccc
     1681 tcgattcgcc ctatgcggtg ctcaaagccg ggctcctcat atcggaagtg ccgcgcatat
     1741 tcctcgcgct cgaaagactg gcggataaat tcgggatacg cgcccgcgcg tcggcaaggg
     1801 cggcaaacgg cgtcgtgatc atttcggtcg cgggcgctaa tgagaccgtg ctcaccgccg
     1861 tcaaggagct gagggaattt tccgaatccg cggggggcag tctggtcgtg agcgaagctt
     1921 ccccggatat taagcccggt atcgacgtct ggggcacgat cggctcctcc ctcaatatga
     1981 tgaagcgaat aaaatcgggt ttcgatcccg gcaacatcct caatccgggc aggcttttct
     2041 gacataaaat cggtctttaa atcccttgca cggcactccc cgcttggtta ggctattccc
     2101 tttccatgac tgaaaggcct ctgtccggag aattttatga tccgtagaga cgataccgac
     2161 gggtggattc ttatcacaca gtacgaccac gctgtattgt ccggcaggat catggatcgg
     2221 tggggcaacg agaccttctc ttcccccgac cccggcgagg aaacgctctt cgccgtcagg
     2281 gagcacgact gcggctggga ggattgggac tcctccccga aaatatatcc cgagaacggt
     2341 tatccggcga atttca
//
